title: Characterization of membrane progestin receptor α (mPRα) of the medaka and role in the induction of oocyte maturation.
abstract: Oocyte maturation in medaka is induced by the maturation-inducing hormone (MIH) via its membrane receptor. The most likely candidates for the membrane receptor are membrane progestin receptors (mPRs). In order to characterize the mPRα subtype of medaka, a human cell line expressing the mPRα gene of medaka was established and its steroid binding property was assessed. The α subtype exhibited high binding affinity for 17,20β-DHP, the MIH in medaka. Treatment with a morpholino antisense oligonucleotide to mPRα blocked oocyte maturation in vivo. These results suggest that the medaka mPRα protein acts as an intermediary during MIH-induced oocyte maturation in medaka in a manner similar to that described previously for fish species.
authors.affiliation: Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation Shizuoka University.
authors.forename: Shimi Rani
authors.initials: SR
authors.lastname: Roy
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Md Rubel
authors.initials: MR
authors.lastname: Rana
authors.forename: Mikiko
authors.initials: M
authors.lastname: Nakashima
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Tokumoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D009990:Oryzias / Q000235:genetics / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D043562:Receptors, G-Protein-Coupled; D011980:Receptors, Progesterone; C513556:PAQR7 protein, human