title: A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples.
abstract: BACKGROUND
  Different methods available for extraction of human genomic DNA suffer from one or more drawbacks including low yield, compromised quality, cost, time consumption, use of toxic organic solvents, and many more. Herein, we aimed to develop a method to extract DNA from 500 μL of fresh or frozen human blood.
  
  
  METHODS
  Five hundred microliters of fresh and frozen human blood samples were used for standardization of the extraction procedure. Absorbance at 260 and 280 nm, respectively, (A260/A280) were estimated to check the quality and quantity of the extracted DNA sample. Qualitative assessment of the extracted DNA was checked by Polymerase Chain reaction and double digestion of the DNA sample.
  
  
  RESULTS
  Our protocol resulted in average yield of 22±2.97 μg and 20.5±3.97 μg from 500 μL of fresh and frozen blood, respectively, which were comparable to many reference protocols and kits.
  
  
  CONCLUSIONS
  Besides yielding bulk amount of DNA, our protocol is rapid, economical, and avoids toxic organic solvents such as Phenol. Due to unaffected quality, the DNA is suitable for downstream applications. The protocol may also be useful for pursuing basic molecular researches in laboratories having limited funds.
authors.affiliation: Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri, India.
authors.forename: Pokhraj
authors.identifier: http://orcid.org/0000-0003-0952-8111
authors.initials: P
authors.lastname: Guha
authors.affiliation: Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri, India.
authors.forename: Avishek
authors.initials: A
authors.lastname: Das
authors.affiliation: Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri, India.
authors.forename: Somit
authors.initials: S
authors.lastname: Dutta
authors.affiliation: Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri, India.
authors.forename: Tapas Kumar
authors.identifier: http://orcid.org/0000-0002-2667-6952
authors.initials: TK
authors.lastname: Chaudhuri
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D004247:DNA / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007194:India; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: DNA extraction; cost effective; high yield; human blood