title: Biochemical Methods to Monitor Autophagic Responses in Plants.
abstract: The study of autophagy in plants is rapidly increasing, due to its pivotal and fundamental roles in responding to stressful stimuli, recycling nutrients during senescence, and maintaining growth under normal conditions. Assays for detecting autophagy in plants have generally been based on microscopic observations, providing qualitative information on autophagy activity. Here, we discuss biochemical assays for detecting autophagy, which have the potential for providing more quantitative information, with a focus on immunoblotting with antibodies against ATG8, NBR1, or epitope tags fused to ATG proteins.
authors.affiliation: Iowa State University, Ames, IA, United States.
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Iowa State University, Ames, IA, United States.
authors.forename: Y
authors.initials: Y
authors.lastname: Mugume
authors.affiliation: Iowa State University, Ames, IA, United States. Electronic address: bassham@iastate.edu.
authors.forename: D C
authors.initials: DC
authors.lastname: Bassham
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000378:metabolism; D001343:Autophagy; D000071190:Autophagy-Related Protein 8 Family / Q000032:analysis* / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D015151:Immunoblotting / Q000379:methods*; D059748:Proteolysis
chemical_list: C000597073:ATG8 protein, Arabidopsis; D029681:Arabidopsis Proteins; D000071190:Autophagy-Related Protein 8 Family; D002352:Carrier Proteins; C568852:NBR1 protein, Arabidopsis
keywords: ATG8; Antibodies; Arabidopsis; Autophagy; Biochemical assays; Immunoblotting