title: Intravital characterization of tumor cell migration in pancreatic cancer.
abstract: Curing pancreatic cancer is difficult as metastases often determine the poor clinical outcome. To gain more insight into the metastatic behavior of pancreatic cancer cells, we characterized migratory cells in primary pancreatic tumors using intravital microscopy. We visualized the migratory behavior of primary tumor cells of a genetically engineered pancreatic cancer mouse model and found that pancreatic tumor cells migrate with a mesenchymal morphology as single individual cells or collectively as a stream of non-cohesive single motile cells. These findings may improve our ability to conceive treatments that block metastatic behavior.
authors.affiliation: Cancer Genomics Netherlands, Hubrecht Institute-KNAW & University Medical Center Utrecht , Utrecht, the Netherlands.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Beerling
authors.affiliation: University Medical Center Utrecht , Utrecht, the Netherlands.
authors.forename: Ilse
authors.initials: I
authors.lastname: Oosterom
authors.affiliation: Cancer Genomics Netherlands, The Netherlands Cancer Institute , Amsterdam, the Netherlands.
authors.forename: Emile
authors.initials: E
authors.lastname: Voest
authors.affiliation: University Medical Center Utrecht , Utrecht, the Netherlands.
authors.forename: Martijn
authors.initials: M
authors.lastname: Lolkema
authors.affiliation: Cancer Genomics Netherlands, Hubrecht Institute-KNAW & University Medical Center Utrecht , Utrecht, the Netherlands.
authors.forename: Jacco
authors.initials: J
authors.lastname: van Rheenen
keywords: collective migration; intravital microscopy; migration; pancreatic tumors