title: Mitochondrial DNA sequencing demonstrates clonality of peritoneal implants of borderline ovarian tumors.
abstract: Borderline ovarian tumors are rare low malignant potential neoplasms characterized by the absence of stromal invasion, whose main prognostic factors are stage and type of peritoneal implants. The latter are defined as invasive when cell proliferation invades the underlying tissue (peritoneal surface, omentum and intestinal wall), or noninvasive. It is still unknown if these implants are metastatic spread from the primary ovarian mass or a neoplastic transformation de novo of the peritoneal surface. Mitochondrial DNA sequencing was performed to assess clonality in eight patients presenting both borderline ovarian tumors and implants. In 37.5% of the cases, the same mitochondrial DNA mutation was present in both borderline ovarian tumors and the peritoneal implant, being this evidence that implants may arise as a consequence of a spread from a single ovarian site.
authors.affiliation: Department of Medical and Surgical Sciences (DIMEC) - Unit of Medical Genetics, University of Bologna Medical School, Via G. Massarenti 9, 40138, Bologna, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Girolimetti
authors.affiliation: Department of Obstetrics and Gynecology, Oncologic Gynecology Unit, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Pierandrea
authors.initials: P
authors.lastname: De Iaco
authors.affiliation: Department of Obstetrics and Gynecology, Oncologic Gynecology Unit, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Procaccini
authors.affiliation: Unit of Pathology, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Panzacchi
authors.affiliation: Department of Medical and Surgical Sciences (DIMEC) - Unit of Medical Genetics, University of Bologna Medical School, Via G. Massarenti 9, 40138, Bologna, Italy.
authors.forename: Ivana
authors.initials: I
authors.lastname: Kurelac
authors.affiliation: Department of Medical and Surgical Sciences (DIMEC) - Unit of Medical Genetics, University of Bologna Medical School, Via G. Massarenti 9, 40138, Bologna, Italy.
authors.forename: Laura Benedetta
authors.initials: LB
authors.lastname: Amato
authors.affiliation: Department of Obstetrics and Gynecology, Oncologic Gynecology Unit, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Dondi
authors.affiliation: Unit of Oncology and Transplant Pathology, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Caprara
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Ceccarelli
authors.affiliation: Unit of Pathology, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Santini
authors.affiliation: Department of Pharmacy and Biotechnology, University of Bologna, 40138, Bologna, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Porcelli
authors.affiliation: Department of Obstetrics and Gynecology, Oncologic Gynecology Unit, University Hospital S.Orsola-Malpighi, 40138, Bologna, Italy.
authors.forename: Anna Myriam
authors.initials: AM
authors.lastname: Perrone
authors.affiliation: Department of Medical and Surgical Sciences (DIMEC) - Unit of Medical Genetics, University of Bologna Medical School, Via G. Massarenti 9, 40138, Bologna, Italy. giuseppe.gasparre@gmail.com.
authors.forename: Giuseppe
authors.identifier: 0000-0003-1229-9006
authors.initials: G
authors.lastname: Gasparre
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D060965:Clonal Evolution; D004272:DNA, Mitochondrial; D005260:Female; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D010534:Peritoneal Neoplasms / Q000556:secondary*; D011379:Prognosis; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial
keywords: Borderline ovarian tumors; Gynecological cancer; Mitochondrial DNA mutations; Peritoneal implants