title: Hair growth promoting effect of white wax and policosanol from white wax on the mouse model of testosterone-induced hair loss.
abstract: White wax (WW) has been traditionally used to treat hair loss in China. However there has been no reporter WW and its extract responsible for hair growth-promoting effect on androgenetic alopecia. In this paper, we examined the hair growth-promoting effects of WW and policosanol of white wax (WWP) on model animal of androgenetic alopecia and the potential target cell of WW and WWP. WW (1, 10 and 20%) and WWP (0.5, 1 and 2%) were applied topically to the backs of mice. Finasteride (2%) was applied topically as a positive control. MTS assays were performed to evaluate cell proliferation in culture human follicle dermal papilla cells (HFDPCs). The inhibition of WW and WWP for 5α- reductase were tested in Vitro. Results showed more lost hairs were clearly seen in mice treated with TP only and TP plus vehicle. Mice which received TP plus WW and WWP showed less hair loss. WW and WWP showed an outstanding hair growth-promoting activity as reflected by the follicular length, follicular density, A/T ratio, and hair bulb diameter. The optimal treatment effect was observed at 10% WW and 1% WWP, which were better than 2% finasteride treatment. MTS assay results suggested that WW and WWP remarkably increased the proliferation of HFDPCs. Inhibitor assay of 5α- reductase showed that WW and WWP inhibited significantly the conversion of testosterone to dihydrotesterone, and the IC50values of WW and WWP were higher than that of finasteride. In Conclusion, WW and WWP could act against testosterone-induced alopecia in mice, and they promoted hair growth by inhibiting 5α-reductase activity and HFDPCs proliferation. DPCs is the target cell of WW and WWP.
authors.affiliation: Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China.
authors.forename: Zhan-di
authors.initials: ZD
authors.lastname: Wang
authors.affiliation: Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China.
authors.forename: Li-Yi
authors.initials: LY
authors.lastname: Ma
authors.affiliation: Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Ding
authors.affiliation: Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China. Electronic address: cafcxm@139.com.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Chen
mesh_terms: D058891:5-alpha Reductase Inhibitors / Q000494:pharmacology; D000505:Alopecia / Q000517:prevention & control*; D000818:Animals; D005233:Fatty Alcohols / Q000737:chemistry / Q000494:pharmacology*; D018120:Finasteride / Q000494:pharmacology; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D011897:Random Allocation; D043343:Testosterone Propionate / Q000008:administration & dosage / Q000009:adverse effects*; D014885:Waxes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D058891:5-alpha Reductase Inhibitors; D005233:Fatty Alcohols; D010936:Plant Extracts; D014885:Waxes; C080710:policosanol; D018120:Finasteride; D043343:Testosterone Propionate
keywords: 5α- reductase; Androgenetic alopecia; Dermal papilla cells; Hair follicles; Policosanol from white wax; White wax