title: Downregulated XPA promotes carcinogenesis of bladder cancer via impairment of DNA repair.
abstract: Bladder cancer is the most common malignant tumor of urinary system, largely resulting from failure of repair of DNA damage to the environmental insults. The function of XPA in nucleotide excision repair pathway has been well documented. However, participation of XPA in the repair of DNA double-strand break remains unknown. Here, we reported that bladder cancer expressed low XPA levels compared to adjacent non-tumor bladder tissue, and this phenotype was closely associated with chromosomal aberrations. Moreover, downregulated XPA appeared to increase incidence of chromosome aberration. XPA reduction increased cell viability of a bladder cancer cell line RT4, while XPA re-expression decreased the cell viability of RT4 cells. Since high mutation frequency is the basis of mutations of oncogenes and anti-oncogenes, and may be the essence of bladder cancer susceptibility, our study suggests that downregulated XPA may promote carcinogenesis of bladder cancer via impairment of DNA repair.
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhi
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Huixiang
authors.initials: H
authors.lastname: Ji
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Pan
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Zhansong
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Urology Institute of People Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000368:Aged; D016203:CDC2 Protein Kinase; D063646:Carcinogenesis / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D020552:Comet Assay; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D015536:Down-Regulation; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism; D051760:Xeroderma Pigmentosum Group A Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D019926:Cyclin B; D019927:Cyclin E; C468783:H2AX protein, human; D006657:Histones; C497314:XPA protein, human; D051760:Xeroderma Pigmentosum Group A Protein; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases
keywords: DNA repair; XPA; bladder cancer; chromosome aberration