title: Amelioratory effect of coenzyme Q10 on potential human carcinogen Microcystin-LR induced toxicity in mice.
abstract: Microcystins are a group of cyclic heptapeptide toxins produced by cyanobacteria. More than 100 microcystin analogues have been detected, among which microcystin-LR is the most abundant and toxic variant. Present study was designed to reveal whether potential human carcinogen microcystin-LR could imbalance the glycolytic-oxidative-nitrosative status of heart, kidney and spleen of mice and also to explore the amelioratory effect of coenzyme Q10 on microcystin-LR induced toxicity. Microcystin-LR was administered at a dose of 10 μg/kg bw/day, ip for 14 days in male mice. In microcystin-LR treated mice as compared to control, significant increase in the level of lipid peroxidation, hydrogen peroxide, lactate dehydrogenase, nitric oxide with a concomitant decrease in the level of glutathione was observed, suggesting microcystin-LR induced toxicity via induction of oxidative-nitrosative-glycolytic pathway. Although several studies have evaluated numerous antioxidants but still there is no effective chemoprotectant against microcystin-LR induced toxicity. When microcystin-LR treated mice were co-administered coenzyme Q10 (10 mg/kg bw/day, im) for 14 days, it was observed that coenzyme Q10 ameliorates microcystin-LR induced toxicity via modulation of glycolytic-oxidative-nitrosative stress pathway. Thus, the results suggest that coenzyme Q10 has a potential to be developed as preventive agent against microcystin-LR induced toxicity.
authors.affiliation: Department of Zoology, Dr. Harisingh Gour Central University, Sagar, Madhya Pradesh, 470003, India.
authors.forename: Yaqoob
authors.initials: Y
authors.lastname: Lone
authors.affiliation: Department of Zoology, Dr. Harisingh Gour Central University, Sagar, Madhya Pradesh, 470003, India.
authors.forename: Mangla
authors.initials: M
authors.lastname: Bhide
authors.affiliation: Department of Zoology, Dr. Harisingh Gour Central University, Sagar, Madhya Pradesh, 470003, India. Electronic address: rkkoiri@gmail.com.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Koiri
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008387:Marine Toxins; D008815:Mice, Inbred Strains; D052998:Microcystins / Q000633:toxicity*; D015538:Nitrosation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D008387:Marine Toxins; D052998:Microcystins; D020011:Protective Agents; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D007770:L-Lactate Dehydrogenase; C024989:coenzyme Q10; C057862:cyanoginosin LR
keywords: Coenzyme Q10; Glutathione; Lactate dehydrogenase; Lipid peroxidation; Microcystin-LR (MC-LR); Microcystins; Nitric oxide