title: Fission yeast APC/C activators Slp1 and Fzr1 sequentially trigger two consecutive nuclear divisions during meiosis.
abstract: In meiosis, two rounds of nuclear division occur consecutively without DNA replication between the divisions. We isolated a fission yeast mutant in which the nucleus divides only once to generate two spores, as opposed to four, in meiosis. In this mutant, we found that the initiation codon of the slp1+gene is converted to ATA, producing a reduced amount of Slp1. As a member of the Fizzy family of anaphase-promoting complex/cyclosome (APC/C) activators, Slp1 is essential for vegetative growth; however, the mutant allele shows a phenotype only in meiosis. Slp1 insufficiency delays degradation of maturation-promoting factor at the first meiotic division, and another APC/C activator, Fzr1, which acts late in meiosis, terminates meiosis immediately after the delayed first division to produce two viable spores.
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Chikashige
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Yamane
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Kasumi
authors.initials: K
authors.lastname: Okamasa
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Osakada
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tsutsumi
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nagahama
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Fukuta
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.|Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
authors.forename: Tokuko
authors.initials: T
authors.lastname: Haraguchi
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.|Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hiraoka
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism; D015153:Blotting, Western; D064199:Cdc20 Proteins / Q000235:genetics / Q000378:metabolism*; D064200:Cdh1 Proteins / Q000235:genetics / Q000378:metabolism*; D048750:Cell Nucleus Division / Q000235:genetics; D008540:Meiosis; D008856:Microscopy, Fluorescence; D009154:Mutation; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D064199:Cdc20 Proteins; D064200:Cdh1 Proteins; D029702:Schizosaccharomyces pombe Proteins; C577504:slp1 protein, S pombe; D064173:Anaphase-Promoting Complex-Cyclosome
keywords: 
  Schizosaccharomyces pombe
  ; APC/C; Fzr1; Mes1; Slp1; meiosis