title: Tolerance to 1,25 dihydroxyvitamin D3glycosides from Solanum glaucophyllum by the growing pig.
abstract: Solanum glaucophyllum leaves contain high levels of glycosidically bound 1,25 dihydroxyvitamin D3, the most important vitamin D metabolite. The tolerance to this source was evaluated during six weeks with fifty weaned pigs fed increasing levels (0, 2.5, 5, 10 and 20μg 1,25(OH)2D3/kg diet). The diet contained, per kg, 9.7g Ca, 3.5g digestible P and 2000IU cholecalciferol. Ten additional pigs were fed a diet containing 1000IU cholecalciferol/kg, without 1,25(OH)2D3. Weekly plasma and final kidney, bone and urinary mineral contents, bone density and breaking strength served as indicators for possible adverse effects of the supplement. All animals grew well and remained clinically healthy. The measured parameters remained unchanged when 1000 replaced 2000IU cholecalciferol/kg and when 1,25(OH)2D3was fed up to 10μg/kg. Twenty μg 1,25(OH)2D3increased plasma Ca and decreased plasma P from the 2ndand the 4thexperimental week onwards, respectively. Twenty μg 1,25(OH)2D3increased final plasma Ca and 1,25(OH)2D3and reduced final plasma P by respectively 19, 56 and 13%. Twenty μg 1,25(OH)2D3also increased kidney Ca and urinary Ca by 43 and 69%, respectively, reduced bone breaking strength by 12% and tended to decrease bone ash by 3%. To conclude, 2000IU D3was not beneficial compared to 1000IU cholecalciferol; up to 10μg 1,25(OH)2D3per kg diet did not lead to observed adverse effects; 20μg 1,25(OH)2D3altered the homeostatic regulation of Ca and P thus, may lead to first signs of possible adverse effects, such as soft tissue calcification.
authors.affiliation: Agroscope, 1725 Posieux, Switzerland. Electronic address: patrick.schlegel@agroscope.admin.ch.
authors.forename: P
authors.initials: P
authors.lastname: Schlegel
authors.affiliation: Agroscope, 3003 Bern, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Guggisberg
authors.affiliation: Agroscope, 1725 Posieux, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Gutzwiller
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D036061:Solanum glaucophyllum / Q000737:chemistry*; D013552:Swine; D014807:Vitamin D / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D006027:Glycosides; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D002117:Calcitriol; D002118:Calcium
keywords: 1,25 dihydroxyvitamin D(3); Calcium; Phosphorus; Pig; Solanum glaucophyllum; Tolerance