title: The function and transcriptome analysis of a bZIP transcription factor CgAP1 in Colletotrichum gloeosporioides.
abstract: Colletotrichum gloeosporioides is an important pathogen of anthracnose, which is able to infect numerous crops in tropical and subtropical regions, causing great economic losses. To investigate the fungal response to host-generated reactive oxygen species (ROS), we cloned and characterized the CgAP1 gene of C. gloeosporioides. CgAP1 encoded a bZIP transcription factor which had a bZIP DNA-binding domain and two cysteine-rich domains structurally and functionally related to Saccharomyces cerevisiae YAP1. Deletion of CgAP1 in C. gloeosporioides resulted in increasing sensitivity to H2O2, changes in cell wall integrity and loss of pathogenicity. To understand the regulatory network of CgAP1, RNA sequencing was used to identify differentially expressed genes in the CgAP1 mutant. It was shown that several genes involved in ROS detoxification and cell wall integrity were affected by CgAP1. Moreover, CgAP1 was also involved in many biological processes especially ribosome, cellular transport and amino acid metabolism. In conclusion, CgAP1 is an important transcription factor involved in oxidative stress, cell wall integrity and pathogenicity in C. gloeosporioides.
authors.affiliation: Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
authors.forename: Yateng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China. Electronic address: liuzhiqiang80@126.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
authors.forename: Chenghui
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002473:Cell Wall / Q000737:chemistry; D020231:Colletotrichum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D005656:Fungal Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism; D014774:Virulence
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D005656:Fungal Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide
keywords: Colletotrichum gloeosporioides; Pathogenicity; RNA sequencing; Reactive oxygen species; Transcription factor