title: Effect of Fibroblast-Like Cells of Mesenchymal Origin of Cytotoxic Activity of Lymphocytes against NK-Sensitive Target Cells.
abstract: We studied immunosuppressive properties of skin fibroblasts and mesenchymal stromal cells against NK cells. In vitro experiments showed that mesenchymal stromal cells isolated from human umbilical cord and human skin fibroblasts can considerably attenuate cytotoxic activity of NK cells against Jurkat cells sensitive to NK-mediated lysis. NK cells cultured in lymphocyte population exhibited higher cytotoxic activity than isolated NK cells. Mesenchymal stromal cells or fibroblasts added 1:1 to lymphocyte culture almost completely suppressed NK cell cytotoxicity. This suggests that fibroblast-like cells can suppress not only isolated NK cells, but also NK cells in natural cell microenvironment.
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia. alupatov@inbox.ru.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. alupatov@inbox.ru.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Lupatov
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: Ya S
authors.initials: YS
authors.lastname: Kim
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Bystrykh
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Vakhrushev
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Pavlovich
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D002450:Cell Communication / Q000276:immunology*; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D019169:Jurkat Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D061251:Primary Cell Culture; D012867:Skin / Q000166:cytology / Q000276:immunology
chemical_list: D007376:Interleukin-2
keywords: NK cells; fibroblasts; immunosuppression; lymphocytes; mesenchymal stromal cells