title: Analyses of pea necrotic yellow dwarf virus-encoded proteins.
abstract: Pea necrotic yellow dwarf virus (PNYDV) is a multipartite, circular, single-stranded DNA plant virus. PNYDV encodes eight proteins and the function of three of which remains unknown-U1, U2, and U4. PNYDV proteins cellular localization was analyzed by GFP tagging and bimolecular fluorescence complementation (BiFC) studies. The interactions of all eight PNYDV proteins were tested pairwise in planta (36 combinations in total). Seven interactions were identified and two (M-Rep with CP and MP with U4) were characterized further. MP and U4 complexes appeared as vesicle-like spots and were localized at the nuclear envelope and cell periphery. These vesicle-like spots were associated with the endoplasmatic reticulum. In addition, a nuclear localization signal (NLS) was mapped for U1, and a mutated U1 with NLS disrupted localized at plasmodesmata and therefore might also have a role in movement. Taken together, this study provides evidence for previously undescribed nanovirus protein-protein interactions and their cellular localization with novel findings not only for those proteins with unknown function, but also for characterized proteins such as the CP.
authors.affiliation: Lehrstuhl für Biochemie, Department Biologie, Universität Erlangen-Nürnberg, Staudtstr. 5, 91058, Erlangen, Germany. bjoern.krenz@fau.de.
authors.forename: Björn
authors.identifier: http://orcid.org/0000-0003-0645-9923
authors.initials: B
authors.lastname: Krenz
authors.affiliation: Lehrstuhl für Biochemie, Department Biologie, Universität Erlangen-Nürnberg, Staudtstr. 5, 91058, Erlangen, Germany.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Schießl
authors.affiliation: Lehrstuhl für Biochemie, Department Biologie, Universität Erlangen-Nürnberg, Staudtstr. 5, 91058, Erlangen, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Greiner
authors.affiliation: Lehrstuhl für Biochemie, Department Biologie, Universität Erlangen-Nürnberg, Staudtstr. 5, 91058, Erlangen, Germany.
authors.forename: Susanna
authors.initials: S
authors.lastname: Krapp
mesh_terms: D015967:Gene Expression Regulation, Viral; D025682:Nanovirus / Q000235:genetics / Q000378:metabolism*; D019913:Nuclear Localization Signals; D018532:Pisum sativum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D060066:Protein Interaction Maps; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D019913:Nuclear Localization Signals; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins
keywords: Bimolecular fluorescence complementation (BiFC); Nanoviridae; Nanovirus; Pea necrotic yellow dwarf virus