title: Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.
abstract: Incorporating molecular libraries in chemical biology screenings in cultured cells has been successfully used for gene discovery in many cellular processes. It has the unique potential to uncover novel mechanisms of complex cellular biology through the screening of small molecules and protein biologics in relevant cell-based assays. Recent development in the understanding and generation of thermogenic adipocytes provides opportunities for potential anti-obesity therapeutics discovery. In this chapter, we describe screening methods using thermogenic beige cells to identify novel compounds and peptides that activate adipocyte thermogenesis.
authors.affiliation: Department of Biochemistry, Center for Drug Discovery, and Translational Obesity Research Center, Virginia Tech, 340 West Campus Dr., 105 Engel Hall,, Blacksburg, VA, 24061, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Biochemistry, Center for Drug Discovery, and Translational Obesity Research Center, Virginia Tech, 340 West Campus Dr., 105 Engel Hall,, Blacksburg, VA, 24061, USA. binxu@vt.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
mesh_terms: D000069797:Adipocytes, Beige / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D055808:Drug Discovery / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D015203:Reproducibility of Results; D054852:Small Molecule Libraries
chemical_list: D010455:Peptides; D054852:Small Molecule Libraries
keywords: Beige cell; Quantitative PCR; Small molecule and peptide screening; Thermogenic adipocyte