title: Characterisation of the Bordetella pertussis secretome under different media.
abstract: Our understanding of the Bordetella pertussis secretome remains limited including the role of different growth conditions in the secretome. In this study the secretome of L1423, a clinical isolate from the 2008-2012 Australian epidemic, cultured on Stainer-Scholte (SS) and Thalen-IJssel (THIJS) media for 12h was characterised using liquid chromatography-mass spectrometry (LC-MS/MS). In the supernatant, LC-MS/MS identified 260 proteins with 143 bioinformatically predicted to be secreted. Eighty percent of proteins were identified in both media. Proteins secreted were functionally associated with cell surface (41%), pathogenicity (16%) and transport (17%). The most abundant proteins identified were pathogenic proteins including toxins (PtxA and CyaA), adhesins (TcfA) and type III secretion (T3SS) proteins. There were 46 proteins found uniquely in THIJS including 8 virulence associated proteins. These included T3SS proteins, adhesins (FhaL and FhaS) and a putative toxin (BP1251). Nine proteins were found uniquely in SS and these were metabolic and transport-related proteins. None of the unique proteins detected in SS were known to be virulence associated. This study found that THIJS promotes secretion of virulence factors based on the number of unique virulence proteins found and may be a growth media of choice for the study of B. pertussis virulence and vaccine development.
  
  
  
  Over the past two decades, the number of B. pertussis notifications has risen despite vaccination. There is a greater need to understand the biology behind B. pertussis infections. The secretome of B. pertussis in two different media was characterised using LC-MS/MS. The results showed that THIJS promotes secretion of importance virulence factors which may be important for the development of vaccines.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Laurence Don Wai
authors.initials: LDW
authors.lastname: Luu
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Sophie
authors.initials: S
authors.lastname: Octavia
authors.affiliation: Mark Wainwright Analytical Centre, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Mark Wainwright Analytical Centre, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Raftery
authors.affiliation: Centre for Infectious Diseases and Microbiology-Public Health, Institute of Clinical Pathology and Medical Research - Pathology West, Westmead Hospital, New South Wales, Australia; Marie Bashir Institute for Infectious Diseases and Biosecurity, Sydney Medical School, University of Sydney, New South Wales, Australia.
authors.forename: Vitali
authors.initials: V
authors.lastname: Sintchenko
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia. Electronic address: r.lan@unsw.edu.au.
authors.forename: Ruiting
authors.initials: R
authors.lastname: Lan
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001886:Bordetella pertussis / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome
keywords: Bordetella pertussis; Proteomics; Secretome