title: A Comprehensive Analysis of Metabolomics and Transcriptomics in Cervical Cancer.
abstract: Cervical cancer (CC) still remains a common and deadly malignancy among females in developing countries. More accurate and reliable diagnostic methods/biomarkers should be discovered. In this study, we performed a comprehensive analysis of metabolomics (285 samples) and transcriptomics (52 samples) on the potential diagnostic implication and metabolic characteristic description in cervical cancer. Sixty-two metabolites were different between CC and normal controls (NOR), in which 5 metabolites (bilirubin, LysoPC(17:0), n-oleoyl threonine, 12-hydroxydodecanoic acid and tetracosahexaenoic acid) were selected as candidate biomarkers for CC. The AUC value, sensitivity (SE), and specificity (SP) of these 5 biomarkers were 0.99, 0.98 and 0.99, respectively. We further analysed the genes in 7 significantly enriched pathways, of which 117 genes, that were expressed differentially, were mainly involved in catalytic activity. Finally, a fully connected network of metabolites and genes in these pathways was built, which can increase the credibility of our selected metabolites. In conclusion, our biomarkers from metabolomics could set a path for CC diagnosis and screening. Our results also showed that variables of both transcriptomics and metabolomics were associated with CC.
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Gynecology Oncology, the Tumor Hospital, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Bairong
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Gynecology Oncology, the Tumor Hospital, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Natural Products, Jiangsu Key Laboratory of TCM Evaluation; Translational Research Department of Complex Prescription of TCM, Pharmaceutical University, 639 Longmian Road, Nanjing 211198, P.R. China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Yin
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Junnan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Libing
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fan
authors.affiliation: Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Harman S
authors.initials: HS
authors.lastname: Dhillon
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.|Key Laboratory of Cardiovascular Medicine Research, Harbin Medical University, Ministry of Education, Harbin, 150086, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Gynecology Oncology, the Tumor Hospital, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Ge
authors.initials: G
authors.lastname: Lou
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, 150086, P.R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D055442:Metabolome; D008875:Middle Aged; D012372:ROC Curve; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor