title: Effects of Resistance Training Volume on MMPs in Circulation, Muscle and Adipose Tissue.
abstract: The aim of this study was to investigate the effects of different resistance training (RT) volumes on MMP activity in skeletal muscle, visceral adipose tissue and circulation. 21 Wistar rats were randomly divided into 3 groups (n=7 per group): sedentary control (SC); RT with 4 ladder climbs (RT-4; 50, 75, 90 and 100% of their maximal carrying capacity) and RT with 8 ladder climbs (RT-8 with 2 sets for each load). The 8-week RT consisted of climbing a 1.1-m vertical ladder with weights secured to the animals' tails. MMP-2 and -9 activity were analyzed by zymography. RT-8 displayed higher active MMP-2 activity as compared with SC and RT-4 in skeletal muscle (p<0.05). There was no significant difference between groups for pro and intermediate-MMP-2 activity in visceral adipose tissue, while RT-8 presented lower active MMP-2 activity as compared with SC (p<0.05). Plasma pro and active MMP-2 and MMP-9 activity was lower in RT-8 as compared with RT-4 (p<0.05). These results suggest that higher volume RT up-regulates MMP-2 activity in skeletal muscle, while down-regulating MMP-2 in visceral adipose tissue. Moreover, it induces a decrease of MMP-2, 9 activity in circulation. Different tissue and circulatory MMP responses to RT may result in specific remodeling.
authors.affiliation: Graduate program of Physical Education, Catholic University of Brasilia, Distrito Federal, Brazil.
authors.forename: Ivo Vieira
authors.initials: IV
authors.lastname: de Sousa Neto
authors.affiliation: Graduate program of Physical Education, Catholic University of Brasilia, Distrito Federal, Brazil.
authors.forename: Ramires Alsamir
authors.initials: RA
authors.lastname: Tibana
authors.affiliation: Graduate program of Physical Education, Catholic University of Brasilia, Distrito Federal, Brazil.
authors.forename: Dahan
authors.initials: D
authors.lastname: da Cunha Nascimento
authors.affiliation: Graduate program of Physical Education, Catholic University of Brasilia, Distrito Federal, Brazil.
authors.forename: Denis César Leite
authors.initials: DC
authors.lastname: Vieira
authors.affiliation: Graduate program of Rehabilitation Sciences and Technology of Health, University of Brasilia, Distrito Federal, Brazil.
authors.forename: Joao Luiz Quagliotti
authors.initials: JL
authors.lastname: Durigan
authors.affiliation: Graduate program of Physical Education, Catholic University of Brasilia, Distrito Federal, Brazil.
authors.forename: Guilherme Borges
authors.initials: GB
authors.lastname: Pereira
authors.affiliation: Department of Kinesiology and Nutrition Sciences, University of Nevada, Las Vegas, NV, United States of America.
authors.forename: James Wilfred
authors.initials: JW
authors.lastname: Navalta
authors.affiliation: Graduate program of Rehabilitation Sciences and Technology of Health, University of Brasilia, Distrito Federal, Brazil.
authors.forename: Rita
authors.initials: R
authors.lastname: de Cássia Marqueti
authors.affiliation: Graduate program of Physical Education, Catholic University of Brasilia, Distrito Federal, Brazil.
authors.forename: Jonato
authors.initials: J
authors.lastname: Prestes
mesh_terms: D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood* / Q000502:physiology; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology*; D010805:Physical Conditioning, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D055070:Resistance Training
chemical_list: D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat