title: Bartonella species pathogenic for humans infect pets, free-ranging wild mammals and their ectoparasites in the Caatinga biome, Northeastern Brazil: a serological and molecular study.
abstract: This study verified the occurrence of Bartonella spp. in dogs, cats, wild mammals and their ectoparasites in Petrolina and Lagoa Grande Counties, Pernambuco, located in a semi-arid region in Northeastern Brazil. Anti-Bartonella spp. antibodies were detected by indirect immunofluorescence assay (IFA) in 24.8% of dogs (27/109) and in 15% of cats (6/40). Bartonella sp. DNA was identified by PCR performed on DNA extracted from blood and ectoparasites using primers targeting Bartonella sp. gltA and ribC genes in 100% (9/9) of Pulex irritans from Cerdocyon thous, 57.4% (35/61) of P. irritans from dogs, 2.3% (1/43) of Ctenocephalides felis felis from dogs, 53.3% (24/45) of C. felis felis from cats, and 10% (1/10) of Polyplax spp. from Thrichomys apereoides. DNA sequencing identified Bartonella clarridgeiae and Bartonella henselae in C. felis felis from cats, Bartonella rochalimae in P. irritans from dog and C. thous, and Bartonella vinsoni berkhofii in P. irritans from dog.
authors.affiliation: Universidade Federal do Vale do São Francisco (Univasf), Laboratório de Doenças Parasitárias, Petrolina, PE, Brazil.
authors.forename: Mariana Campos
authors.initials: MC
authors.lastname: Fontalvo
authors.affiliation: Instituto Oswaldo Cruz (FIOCRUZ), Laboratório de Hantaviroses e Rickettsioses, Rio de Janeiro, RJ, Brazil.
authors.forename: Alexsandra Rodrigues de Mendonça
authors.initials: ARM
authors.lastname: Favacho
authors.affiliation: Universidade Federal do Vale do São Francisco (Univasf), Laboratório de Doenças Parasitárias, Petrolina, PE, Brazil.
authors.forename: Andreina de Carvalho
authors.initials: AC
authors.lastname: Araujo
authors.affiliation: Universidade Federal do Vale do São Francisco (Univasf), Laboratório de Doenças Parasitárias, Petrolina, PE, Brazil.
authors.forename: Naylla Mayana Dos
authors.initials: NMD
authors.lastname: Santos
authors.affiliation: Universidade Federal do Vale do São Francisco (Univasf), Laboratório de Doenças Parasitárias, Petrolina, PE, Brazil.
authors.forename: Glauber Meneses Barboza de
authors.initials: GMB
authors.lastname: Oliveira
authors.affiliation: Universidade Federal de Mato Grosso, Laboratório de Virologia e Rickettsioses, Cuiabá, MT, Brazil.
authors.forename: Daniel Moura
authors.initials: DM
authors.lastname: Aguiar
authors.affiliation: Instituto Oswaldo Cruz (FIOCRUZ), Laboratório de Hantaviroses e Rickettsioses, Rio de Janeiro, RJ, Brazil.
authors.forename: Elba Regina Sampaio de
authors.initials: ERS
authors.lastname: Lemos
authors.affiliation: Universidade Federal do Vale do São Francisco (Univasf), Laboratório de Doenças Parasitárias, Petrolina, PE, Brazil. Electronic address: horta.mc@hotmail.com.
authors.forename: Mauricio Claudio
authors.initials: MC
authors.lastname: Horta
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology / Q000469:parasitology; D001473:Bartonella / Q000235:genetics / Q000302:isolation & purification*; D001474:Bartonella Infections / Q000453:epidemiology / Q000662:veterinary*; D001938:Brazil; D046028:Canidae / Q000382:microbiology / Q000469:parasitology; D002415:Cats; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs; D058267:Flea Infestations / Q000662:veterinary*; D019084:Fluorescent Antibody Technique, Indirect; D010373:Lice Infestations / Q000662:veterinary*; D008394:Marsupialia / Q000382:microbiology / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012377:Rodentia / Q000382:microbiology / Q000469:parasitology; D013984:Tick Infestations / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial
keywords: Bartonella; Brazil; Flea; Lice; Tick