title: Methods to Quantify PAMP-Triggered Oxidative Burst, MAP Kinase Phosphorylation, Gene Expression, and Lignification in Brassicas.
abstract: Quantitative disease resistance (QDR) based on PAMP-triggered immunity (PTI) could be durable and effective against many pathogens (broad spectrum). Development of methods to evaluate PTI responses in crops could therefore accelerate breeding for durable QDR. Most PTI-studies involved model plants such as Arabidopsis and Nicotiana benthamiana or cell cultures, and cannot be directly applied to diverse germplasm of crop plants.We developed methods to measure PTI in Brassica crop species (Lloyd et al., Mol Plant Microbe Interact 27:286-295, 2014) which we have elaborated and expanded here to enable their use for screening and evaluating germplasm for potential QDR in breeding programs.
authors.affiliation: Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Lloyd
authors.affiliation: Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Ridout
authors.affiliation: Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. henk-jan.schoonbeek@jic.ac.uk.
authors.forename: Henk-Jan
authors.initials: HJ
authors.lastname: Schoonbeek
mesh_terms: D001937:Brassica / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology*; D010766:Phosphorylation; D010935:Plant Diseases / Q000276:immunology; D057865:Plant Immunity; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000069452:Pathogen-Associated Molecular Pattern Molecules; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D020928:Mitogen-Activated Protein Kinases
keywords: Brassica napus; Brassica oleracea; Crop plants; Lignin; PAMP-triggered immunity; Quantitative disease resistance; Reactive oxygen species