title: Regulation of visual Wulst cell responsiveness by imprinting causes stimulus-specific activation of rostral cells.
abstract: Imprinting behaviour in chicks can be induced exclusively during a short period after hatching. During this period, visual information on the imprinting stimulus is conveyed to the visual Wulst (VW) in the telencephalon, which corresponds to the visual cortex of mammals, and then to the memory-storing region known as the intermediate medial mesopallium. These two regions are indispensable for imprinting. We previously showed that imprinting training altered the response pattern of the VW to the imprinting stimulus; however, the precise distribution of cells and the mechanism involved with this altered response remains unclear. Here we showed that a specific population of rostral VW cells responded to the imprinting stimulus by analysing the subcellular localization of Arc/arg3.1 transcripts in VW cells. GABAergic parvalbumin (PV) cells are abundant in the dorsal region of this area, and imprinting training doubled the number of activated PV-positive neurons. An injection of bicuculline, a GABA(A) receptor antagonist, in the dorsal VW disturbed the rostral distribution of responsive cells and thus resulted in a lack of imprinting. These results suggest that activated PV cells restrict VW cells response to dorsal area to form a specific imprinting pathway.
authors.affiliation: College of Liberal Arts and Sciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan.|Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Nakamori
authors.affiliation: College of Liberal Arts and Sciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kato
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.affiliation: Laboratory of Molecular Neuroscience, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan.|The Center for Brain Integration Research, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan.
authors.forename: Kohichi
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: College of Liberal Arts and Sciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ohki-Hamazaki
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D001921:Brain / Q000473:pathology; D002642:Chick Embryo; D002645:Chickens; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D059330:GABAergic Neurons / Q000166:cytology / Q000378:metabolism*; D007173:Imprinting, Psychological / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D010364:Pattern Recognition, Visual; D010775:Photic Stimulation; D021381:Protein Transport; D009435:Synaptic Transmission / Q000187:drug effects; D013687:Telencephalon / Q000378:metabolism; D014795:Visual Pathways / Q000502:physiology
chemical_list: D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; C092069:activity regulated cytoskeletal-associated protein; D001640:Bicuculline