title: Phenotypic and Functional Characterization of Peripheral Blood Lymphocytes from Various Age- and Sex-Specific Groups of Owl Monkeys (Aotus nancymaae).
abstract: Owl monkeys (Aotus nancymaae) are New World NHP that serve an important role in vaccine development and as a model for human disease conditions such as malaria. Despite the past contributions of this animal model, limited information is available about the phenotype and functional properties of peripheral blood lymphocytes in reference to sex and age. Using a panel of human antibodies and a set of standardized human immune assays, we identified and characterized various peripheral blood lymphocyte subsets, evaluated the immune functions of T cells, and analyzed cytokines relative to sex and age in healthy owl monkeys. We noted age- and sex-dependent changes in CD28+ (an essential T cell costimulatory molecule) and CD95+ (an apoptotic surface marker) T cells and various levels of cytokines in the plasma. In immune assays of freshly isolated peripheral blood mononuclear cells, IFNγ and perforin responses were significantly higher in female than in male monkeys and in young adults than in juvenile and geriatric groups, despite similar lymphocyte (particularly T cell) populations in these groups. Our current findings may be useful in exploring Aotus monkeys as a model system for the study of aging, susceptibility to infectious diseases, and age-associated differences in vaccine efficacy, and other challenges particular to pediatric and geriatric patients.
authors.affiliation: Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas;, Email: pnehete@mdanderson.org.
authors.forename: Pramod N
authors.initials: PN
authors.lastname: Nehete
authors.affiliation: Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Texas.
authors.forename: Bharti P
authors.initials: BP
authors.lastname: Nehete
authors.affiliation: Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Texas.
authors.forename: Sriram
authors.initials: S
authors.lastname: Chitta
authors.affiliation: Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas.
authors.forename: Lawrence E
authors.initials: LE
authors.lastname: Williams
authors.affiliation: Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Texas.
authors.forename: Christian R
authors.initials: CR
authors.lastname: Abee
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016645:Aotidae / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D008297:Male; D054353:Perforin / Q000276:immunology; D010641:Phenotype; D011897:Random Allocation; D012737:Sex Factors; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D054353:Perforin; D007371:Interferon-gamma