title: Aberrant expressions of endometrial Id3 and CTLA-4 are associated with unexplained repeated implantation failure and recurrent miscarriage.
abstract: Inhibitor of DNA-binding protein 3 (Id3) is required for tumor angiogenesis and regulatory T-cell generation. However, the involvement of Id3 in unexplained repeated implantation failure (RIF) and recurrent miscarriage (RM) remains poorly understood. Immunohistochemistry was used to identify Id3, CD34, CTLA-4, and FOXP3 in the endometrium taken from the women with RIF (n=16), RM (n=16) and matched controls (n=8). The images were acquired and analyzed by the Vectra®automated quantitative pathology imaging system. Percentage of Id3+cells was significantly higher in the endometrium of women with RIF and RM compared with controls. The numbers of Id3+and CD34+vessels in the endometrium were positively correlated in control but not in RIF or RM. Percentages of CTLA-4+cells, but not FOXP3+cells, were significantly increased in the endometrium of RIF and RM women than those in controls. We found aberrant expressions of endometrial Id3 and CTLA-4 in peri-implantation endometrium of women with RIF and RM, suggesting the negative roles of these angiogenesis and immune tolerance markers involving in regulating endometrium receptivity.
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Ding
authors.affiliation: Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
authors.forename: Liang-Hui
authors.initials: LH
authors.lastname: Diao
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Tai-Lang
authors.initials: TL
authors.lastname: Yin
authors.affiliation: Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Yin
authors.affiliation: Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Yan-Xiang
authors.initials: YX
authors.lastname: Cheng
authors.affiliation: Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
authors.forename: Yong
authors.identifier: 0000-0002-6264-283X
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000026:Abortion, Habitual / Q000378:metabolism*; D000328:Adult; D060908:CTLA-4 Antigen / Q000378:metabolism*; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D051796:Inhibitor of Differentiation Proteins; D009363:Neoplasm Proteins; C075640:ID3 protein, human
keywords: CD34; cytotoxic T-lymphocyte-associated protein 4 (CTLA-4); forkhead box P3 (FOXP3); inhibitor of DNA-binding protein 3 (Id3); recurrent miscarriage (RM); repeated implantation failure (RIF)