title: Localisation of stem cell factor, stanniocalcin-1, connective tissue growth factor and heparin-binding epidermal growth factor in the bovine uterus at the time of blastocyst formation.
abstract: Early embryonic losses before implantation account for the highest rates of reproductive failure in mammals, in particular when in vitro-produced embryos are transferred. In the present study, we used molecular biology techniques (real-time quantitative polymerase chain reaction), classical immunohistochemical staining coupled with confocal microscopy and proteomic analysis (multiple reaction monitoring and western blot analysis) to investigate the role of four growth factors in embryo-uterine interactions during blastocyst development. Supported by a validated embryo transfer model, the study investigated: (1) the expression of stem cell factor (SCF), stanniocalcin-1 (STC1), connective tissue growth factor (CTGF) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) in bovine uterine fluid; (2) the presence of SCF, STC1, CTGF and HB-EGF mRNA and protein in the bovine endometrium and embryos; and (3) the existence of reciprocal regulation between endometrial and embryonic expression of SCF, STC1, CTGF and HB-EGF. The results suggest that these growth factors most likely play an important role during preimplantation embryo development in cattle. The information obtained from the present study can contribute to improving the performance of in vitro culture technology in cattle and other species.
authors.affiliation: Centro de Biotecnología Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, 33394 Gijón, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Muñoz
authors.affiliation: Centro de Biotecnología Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, 33394 Gijón, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Martin
authors.affiliation: Centro de Biotecnología Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, 33394 Gijón, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Carrocera
authors.affiliation: Unidad de Microscopía Fotónica y Proceso de Imágenes, Servicios Científico Técnicos, Universidad de Oviedo, Instituto Universitario de Oncología de Asturias (IUOPA), 33006, Oviedo, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Alonso-Guervos
authors.affiliation: Unidad de Proteomica, Centro de Investigación Médica Aplicada (CIMA) - Universidad de Navarra, 31008, Pamplona, Navarra, Spain.
authors.forename: M I
authors.initials: MI
authors.lastname: Mora
authors.affiliation: Unidad de Proteomica, Centro de Investigación Médica Aplicada (CIMA) - Universidad de Navarra, 31008, Pamplona, Navarra, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Corrales
authors.affiliation: UMR BDR, INRA, ENVA, Université Paris Saclay, 78350, Jouy en Josas, France.
authors.forename: N
authors.initials: N
authors.lastname: Peynot
authors.affiliation: UMR BDR, INRA, ENVA, Université Paris Saclay, 78350, Jouy en Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Giraud-Delville
authors.affiliation: UMR BDR, INRA, ENVA, Université Paris Saclay, 78350, Jouy en Josas, France.
authors.forename: V
authors.initials: V
authors.lastname: Duranthon
authors.affiliation: UMR BDR, INRA, ENVA, Université Paris Saclay, 78350, Jouy en Josas, France.
authors.forename: O
authors.initials: O
authors.lastname: Sandra
authors.affiliation: Centro de Biotecnología Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, 33394 Gijón, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gómez
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D055513:Connective Tissue Growth Factor / Q000378:metabolism*; D010064:Embryo Implantation / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology*; D004717:Endometrium / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D066257:Heparin-binding EGF-like Growth Factor / Q000378:metabolism*; D011247:Pregnancy; D019089:Stem Cell Factor / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D066257:Heparin-binding EGF-like Growth Factor; D019089:Stem Cell Factor; D055513:Connective Tissue Growth Factor; C051795:teleocalcin