title: Osteogenesis and mineralization of mesenchymal stem cells in collagen type I-based recombinant peptide scaffolds.
abstract: Recombinant peptides have the power to harness the inherent biocompatibility of natural macromolecules, while maintaining a defined chemistry for use in tissue engineering. Creating scaffolds from peptides requires stabilization via crosslinking, a process known to alter both mechanics and density of adhesion ligands. The chemistry and mechanics of linear scaffolds from a recombinant peptide based on human collagen type I (RCP) was investigated after crosslinking. Three treatments were compared: dehydrothermal treatment (DHT), hexamethylene diisocyanate (HMDIC), and genipin. With crosslinking, mechanical properties were not significantly altered, ranging from 1.9 to 2.7 kPa. However, the chemistry of the scaffolds was changed, affecting properties such as water uptake, and initial adhesion of human mesenchymal stem cells (hMSCs). Genipin crosslinking supported the lowest adhesion, especially during osteoblastic differentiation. While significantly altered, RCP scaffold chemistry did not affect osteoblastic differentiation of hMSCs. After four weeks in vitro, all scaffolds showed excellent cellular infiltration, with up-regulated osteogenic markers (RUNX2, Osteocalcin, Collagen type I) and mineralization, regardless of the crosslinker. Thus, it appears that, without significant changes to mechanical properties, crosslinking chemistry did not regulate hMSC differentiation on scaffolds from recombinant peptides, a growing class of materials with the ability to expand the horizons of regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1856-1866, 2017.
authors.affiliation: Department of Life Science, FUJIFILM Manufacturing Europe B.V, Oudenstaart 1, Tilburg, Netherlands.
authors.forename: Kendell M
authors.identifier: http://orcid.org/0000-0003-2606-4136
authors.initials: KM
authors.lastname: Pawelec
authors.affiliation: Translational Center Würzburg 'Regenerative Therapies in Oncology and Musculoskeletal Disease', Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB, Wüerzburg, Germany.
authors.forename: Davide
authors.initials: D
authors.lastname: Confalonieri
authors.affiliation: Department Tissue Engineering and Regenerative Medicine (TERM), University Hospital Wuerzburg, Wuerzburg, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Ehlicke
authors.affiliation: Department of Life Science, FUJIFILM Manufacturing Europe B.V, Oudenstaart 1, Tilburg, Netherlands.
authors.forename: Huibert A
authors.initials: HA
authors.lastname: van Boxtel
authors.affiliation: Translational Center Würzburg 'Regenerative Therapies in Oncology and Musculoskeletal Disease', Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB, Wüerzburg, Germany.|Department Tissue Engineering and Regenerative Medicine (TERM), University Hospital Wuerzburg, Wuerzburg, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Walles
authors.affiliation: Department of Life Science, FUJIFILM Manufacturing Europe B.V, Oudenstaart 1, Tilburg, Netherlands.
authors.forename: Sebastiaan G J M
authors.initials: SGJM
authors.lastname: Kluijtmans
mesh_terms: D000368:Aged; D002113:Calcification, Physiologic; D024042:Collagen Type I / Q000737:chemistry*; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D010012:Osteogenesis; D010455:Peptides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D010455:Peptides; D011994:Recombinant Proteins
keywords: crosslinking; differentiation; osteogenesis; recombinant collagen; stem cells