title: The rapid A-Ciresponse: photosynthesis in the phenomic era.
abstract: Phenotyping for photosynthetic gas exchange parameters is limiting our ability to select plants for enhanced photosynthetic carbon gain and to assess plant function in current and future natural environments. This is due, in part, to the time required to generate estimates of the maximum rate of ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) carboxylation (Vc,max) and the maximal rate of electron transport (Jmax) from the response of photosynthesis (A) to the CO2concentration inside leaf air spaces (Ci). To relieve this bottleneck, we developed a method for rapid photosynthetic carbon assimilation CO2responses [rapid A-Ciresponse (RACiR)] utilizing non-steady-state measurements of gas exchange. Using high temporal resolution measurements under rapidly changing CO2concentrations, we show that RACiR techniques can obtain measures of Vc,maxand Jmaxin ~5 min, and possibly even faster. This is a small fraction of the time required for even the most advanced gas exchange instrumentation. The RACiR technique, owing to its increased throughput, will allow for more rapid screening of crops, mutants and populations of plants in natural environments, bringing gas exchange into the phenomic era.
authors.affiliation: Department of Biology, The University of New Mexico, Albuquerque, NM, 87104, USA.|Department of Biology, The University of Western Ontario, London, Ontario, N6A 5B7, Canada.
authors.forename: Joseph R
authors.identifier: http://orcid.org/0000-0002-7628-4201
authors.initials: JR
authors.lastname: Stinziano
authors.affiliation: LI-COR Inc., Lincoln, NE, 68504, USA.|School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
authors.forename: Patrick B
authors.initials: PB
authors.lastname: Morgan
authors.affiliation: LI-COR Inc., Lincoln, NE, 68504, USA.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Lynch
authors.affiliation: LI-COR Inc., Lincoln, NE, 68504, USA.|School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Saathoff
authors.affiliation: LI-COR Inc., Lincoln, NE, 68504, USA.
authors.forename: Dayle K
authors.initials: DK
authors.lastname: McDermitt
authors.affiliation: Department of Biology, The University of New Mexico, Albuquerque, NM, 87104, USA.
authors.forename: David T
authors.identifier: http://orcid.org/0000-0003-0964-9335
authors.initials: DT
authors.lastname: Hanson
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism*; D010641:Phenotype; D010788:Photosynthesis; D032107:Populus / Q000378:metabolism*
chemical_list: D002245:Carbon Dioxide
keywords: A-Ci; C3; CO2; carboxylation; gas exchange; phenotyping; photosynthesis