title: Polymorphism of DNA repair gene XPD Lys751Gln and chromosome aberrations in lymphocytes of thyroid cancer patients exposed to ionizing radiation due to the Chornobyl accident.
abstract: The aim of this work was to analyze the relationship between polymorphisms of DNA repair gene XPD Lys751Gln and frequency and spectrum of chromosome aberrations in the culture of peripheral blood lymphocytes of thyroid cancer (TC) patients having been exposed to ionizing radiation due to the Chornobyl accident.
  
  
  METHODS
  XPD Lys751Gln polymorphisms were detected by polymerase chain reaction in 102 TC patients including 38 patients exposed to ionizing radiation due to Chornobyl disaster (Chornobyl recovery workers, evacuees, and the residents of contaminated areas), 64 patients without history of ionizing radiation exposure and 45 healthy residents of Ukraine as control group.
  
  
  RESULTS
  In homozygous carriers of the minor allele XPD Gln751Gln, exposed to ionizing radiation, the significantly increased risk of TC (odds ratio = 3.66; p = 0.03; 95% confidence interval 1.04-12.84) was found. Among evacuees and residents of contaminated areas, homozygous carriers of the minor allele variants of XPD gene were characterized by the high level of spontaneous chromosome aberrations. TC patients without history of ionizing radiation exposure, being homozygous carriers of the allele XPD Lys751Lys, had significantly reduced frequency of chromosome-type aberrations.
  
  
  CONCLUSIONS
  The carriage of homozygous minor allele of DNA repair gene XPD Gln751Gln is a risk factor for TC in persons from Ukrainian population exposed to ionizing radiation and is associated with the increased levels of chromosomal instability. This article is a part of a Special Issue entitled "The Chornobyl Nuclear Accident: Thirty Years After".
authors.affiliation: State Institution «National Research Centre for Radiation Medicine of National Academy of Medical Sciences of Ukraine», Kyiv 04050, Ukraine.
authors.forename: V M
authors.initials: VM
authors.lastname: Shkarupa
authors.affiliation: State Institution «National Research Centre for Radiation Medicine of National Academy of Medical Sciences of Ukraine», Kyiv 04050, Ukraine.
authors.forename: O Y
authors.initials: OY
authors.lastname: Mishcheniuk
authors.affiliation: State Institution «Institute of Hereditary Pathology of National Academy of Medical Sciences of Ukraine, Lviv 79000, Ukraine.
authors.forename: S O
authors.initials: SO
authors.lastname: Henyk-Berezovska
authors.affiliation: Ukrainian Scientific and Practical Center of Endocrine Surgery, Transplantation of Endocrine Organs and Tissues of the Ministry of Health of Ukraine, Kyiv 01021, Ukraine.
authors.forename: V O
authors.initials: VO
authors.lastname: Palamarchuk
authors.affiliation: State Institution «National Research Centre for Radiation Medicine of National Academy of Medical Sciences of Ukraine», Kyiv 04050, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Klymenko
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D016022:Case-Control Studies; D047852:Chernobyl Nuclear Accident; D002869:Chromosome Aberrations / Q000528:radiation effects*; D003062:Codon; D004260:DNA Repair / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects*; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D011839:Radiation, Ionizing; D013964:Thyroid Neoplasms / Q000209:etiology*; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D003062:Codon; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human