title: Synthetic Biomarker Design by Using Analyte-Responsive Acetaminophen.
abstract: The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte-responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof-of-concept, we designed hydrogen peroxide (H2O2)-responsive APAP (HR-APAP) and succeeded in H2O2detection with cellular and animal experiments. In fact, for blood samples following HR-APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR-APAP] accurately reflects in vivo differences in H2O2levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nishihara
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Joe
authors.initials: J
authors.lastname: Inoue
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Tabata
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Shinnosuke
authors.initials: S
authors.lastname: Murakami
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: National Institute of Technology, Tsuruoka College, 104 Sawada, Inooka, Tsuruoka, Yamagata, 997-8511, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Saito
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.affiliation: Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
mesh_terms: D000082:Acetaminophen / Q000378:metabolism*; D018712:Analgesics, Non-Narcotic / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D016877:Oxidants / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D018712:Analgesics, Non-Narcotic; D015415:Biomarkers; D008070:Lipopolysaccharides; D016877:Oxidants; D000082:Acetaminophen; D006861:Hydrogen Peroxide
keywords: acetaminophen; hydrogen peroxide; liquid chromatography; mass spectrometry; synthetic biomarker