title: Reversible DNA micro-patterning using the fluorous effect.
abstract: We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.
authors.affiliation: Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK. Alasdair.clark@glasgow.ac.uk.
authors.forename: Gabriella E
authors.identifier: http://orcid.org/0000-0002-1898-8112
authors.initials: GE
authors.lastname: Flynn
authors.affiliation: WestCHEM & Department of Pure & Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK. glenn.burley@strath.ac.uk.
authors.forename: Jamie M
authors.initials: JM
authors.lastname: Withers
authors.affiliation: Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK. Alasdair.clark@glasgow.ac.uk.
authors.forename: Gerard
authors.initials: G
authors.lastname: Macias
authors.affiliation: Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK. Alasdair.clark@glasgow.ac.uk.
authors.forename: Justin R
authors.initials: JR
authors.lastname: Sperling
authors.affiliation: Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK. Alasdair.clark@glasgow.ac.uk.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Henry
authors.affiliation: Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK. Alasdair.clark@glasgow.ac.uk.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Cooper
authors.affiliation: WestCHEM & Department of Pure & Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK. glenn.burley@strath.ac.uk.
authors.forename: Glenn A
authors.identifier: http://orcid.org/0000-0002-4896-113X
authors.initials: GA
authors.lastname: Burley
authors.affiliation: Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK. Alasdair.clark@glasgow.ac.uk.
authors.forename: Alasdair W
authors.initials: AW
authors.lastname: Clark
mesh_terms: D004247:DNA / Q000737:chemistry*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D061848:Optical Imaging; D013499:Surface Properties
chemical_list: D006845:Hydrocarbons, Fluorinated; D004247:DNA