title: Calcium Homeostasis and Muscle Energy Metabolism Are Modified in HspB1-Null Mice.
abstract: Hsp27-encoded byHspB1-is a member of the small heat shock proteins (sHsp, 12-43 kDa (kilodalton)) family. This protein is constitutively present in a wide variety of tissues and in many cell lines. The abundance of Hsp27 is highest in skeletal muscle, indicating a crucial role for muscle physiology. The protein identified as a beef tenderness biomarker was found at a crucial hub in a functional network involved in beef tenderness. The aim of this study was to analyze the proteins impacted by the targeted invalidation ofHspB1in theTibialis anteriormuscle of the mouse.Comparative proteomics using two-dimensional gel electrophoresis revealed 22 spots that were differentially abundant betweenHspB1-null mice and their controls that could be identified by mass spectrometry. Eighteen spots were more abundant in the muscle of the mutant mice, and four were less abundant. The proteins impacted by the absence of Hsp27 belonged mainly to calcium homeostasis (Srl and Calsq1), contraction (TnnT3), energy metabolism (Tpi1, Mdh1, PdhB, Ckm, Pygm, ApoA1) and the Hsp proteins family (HspA9). These data suggest a crucial role for these proteins in meat tenderization. The information gained by this study could also be helpful to predict the side effects of Hsp27 depletion in muscle development and pathologies linked to small Hsps.
authors.affiliation: International Institute of Agronomical Research (INRA)-Vetagro Sup, UMR1213, Saint-Genès-Champanelle F-63122, France. brigitte.picard@clermont.inra.fr.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Picard
authors.affiliation: International Institute of Agronomical Research (INRA)-Vetagro Sup, UMR1213, Saint-Genès-Champanelle F-63122, France. malek.kammoun@utc.fr.
authors.forename: Malek
authors.initials: M
authors.lastname: Kammoun
authors.affiliation: International Institute of Agronomical Research (INRA)-Vetagro Sup, UMR1213, Saint-Genès-Champanelle F-63122, France. gmber2001@yahoo.fr.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Gagaoua
authors.affiliation: International Institute of Agronomical Research (INRA)-Vetagro Sup, UMR1213, Saint-Genès-Champanelle F-63122, France. christiane.barboiron@clermont.inra.fr.
authors.forename: Christiane
authors.initials: C
authors.lastname: Barboiron
authors.affiliation: International Institute of Agronomical Research (INRA)-Vetagro Sup, UMR1213, Saint-Genès-Champanelle F-63122, France. bruno.meunier@clermont.inra.fr.
authors.forename: Bruno
authors.initials: B
authors.lastname: Meunier
authors.affiliation: INRA-Metabolism Exploration Plateform (PFEM), Saint-Genès-Champanelle F-63122, France. Christophe.chambon@clermont.inra.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Chambon
authors.affiliation: International Institute of Agronomical Research (INRA)-Vetagro Sup, UMR1213, Saint-Genès-Champanelle F-63122, France. isabelle.cassar-malek@clermont.inra.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cassar-Malek
keywords: 2D-electrophoresis; HspB1-null mouse; MS-MS; skeletal muscle