title: Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain.
abstract: The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) continues to pose one of the greatest threats to the swine industry. M protein is the most conserved and important structural protein of PRRSV. However, information about the host cellular proteins that interact with M protein remains limited.
  
  
  
  Host cellular proteins that interact with the M protein of HP-PRRSV were immunoprecipitated from MARC-145 cells infected with PRRSV HuN4-F112 using the M monoclonal antibody (mAb). The differentially expressed proteins were identified by LC-MS/MS. The screened proteins were used for bioinformatics analysis including Gene Ontology, the interaction network, and the enriched KEGG pathways. Some interested cellular proteins were validated to interact with M protein by CO-IP.
  
  
  
  The PRRSV HuN4-F112 infection group had 10 bands compared with the control group. The bands included 219 non-redundant cellular proteins that interact with M protein, which were identified by LC-MS/MS with high confidence. The gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway bioinformatic analyses indicated that the identified proteins could be assigned to several different subcellular locations and functional classes. Functional analysis of the interactome profile highlighted cellular pathways associated with protein translation, infectious disease, and signal transduction. Two interested cellular proteins-nuclear factor of activated T cells 45 kDa (NF45) and proliferating cell nuclear antigen (PCNA)-that could interact with M protein were validated by Co-IP and confocal analyses.
  
  
  
  The interactome data between PRRSV M protein and cellular proteins were identified and contribute to the understanding of the roles of M protein in the replication and pathogenesis of PRRSV. The interactome of M protein will aid studies of virus/host interactions and provide means to decrease the threat of PRRSV to the swine industry in the future.
authors.affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.678, Haping street, Xiangfang District, Harbin, 150069, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: National Engineering Research Center of Veterinary Biologics, Harbin, 150001, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agribiotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Dong
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agribiotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.678, Haping street, Xiangfang District, Harbin, 150069, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Peng
authors.affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.678, Haping street, Xiangfang District, Harbin, 150069, China.
authors.forename: Tongqing
authors.initials: T
authors.lastname: An
authors.affiliation: North Guangdong Collaborative Innovation and Development Center of Pig Farming and Disease Control, Shaoguan University, Shaoguan, 512005, China.
authors.forename: Xufu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.678, Haping street, Xiangfang District, Harbin, 150069, China. tzj@hvri.ac.cn.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.678, Haping street, Xiangfang District, Harbin, 150069, China. aci139@sina.com.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Cai
mesh_terms: D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid; D000882:Haplorhini; D054884:Host-Pathogen Interactions; D047468:Immunoprecipitation; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D025941:Protein Interaction Mapping; D013552:Swine; D053719:Tandem Mass Spectrometry; D014763:Viral Matrix Proteins / Q000378:metabolism*
chemical_list: C525999:M protein, porcine reproductive and respiratory syndrome virus; D014763:Viral Matrix Proteins
keywords: Bioinformatics; HP-PRRSV; Host cellular proteins; Interaction; M protein