title: Rapid Analysis of Corni fructus Using Paper Spray-Mass Spectrometry.
abstract: BACKGROUND
  Paper spray-mass spectrometry (PS-MS) is a kind of ambient MS technique for the rapid analysis of samples. Corni fructus has been widely used in traditional Chinese compound preparations and healthy food. However, a number of counterfeits of Corni fructus, such as Crataegi fructus, Lycii fructus, and grape skin are illegally sold in crude herb markets. Therefore, the development of a rapid and high-throughput quality evaluation method is important for ensuring the effectiveness and safety of the crude materials of Corni fructus.
  
  
  OBJECTIVE
  To develop PS-MS chemical profiles and a semi-quantitative method of Corni fructus for quality assessment and control, and species distinction of Corni fructus.
  
  
  METHODS
  Both positive and negative ion PS-MS chemical profiles were constructed for species distinction. The statistical analysis of the chemical profiles was accomplished by principal component analysis (PCA). Rapid semi-quantitative analysis of loganin and morroniside in the extracts of Corni fructus were accomplished by PS-MS.
  
  
  RESULTS
  The profiles of the Corni fructus and Crataegi fructus samples were clearly clustered into two categories. The limit of quantification (LOQ) in the semi-quantitative analysis was 6 μg/mL and 5.6 μg/mL for loganin and morroniside, respectively.
  
  
  CONCLUSIONS
  PS-MS is a simple, rapid, and high-throughput method for the quality control and species distinction of Corni fructus. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha, 410081, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha, 410081, P. R. China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha, 410081, P. R. China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha, 410081, P. R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha, 410081, P. R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.affiliation: Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha, 410081, P. R. China.
authors.forename: Bo
authors.identifier: http://orcid.org/0000-0002-9926-4377
authors.initials: B
authors.lastname: Chen
authors.affiliation: Hunan Analysis and Testing Centre, Changsha, 410004, P. R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
mesh_terms: D029768:Cornus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Corni fructus; chemical profile; paper spray-mass spectrometry; rapid analysis; species distinction