title: Correlation of Bacterial Isolates from Middle Ear and Nasopharynx in Patients with Chronic Suppurative Otitis Media in Ilorin, Nigeria.
abstract: OBJECTIVE
  To determine the association between isolates in the middle ear (ME) and nasopharynx of patients with chronic otitis media in Ilorin, north-central Nigeria.
  
  
  METHODS
  An ethically approved case control study was carried out in the Ear, Nose, and Throat clinic amongst consenting cases using normal subjects as controls. A microbiology investigation form giving the results for otoscopy, aspirate and swabs was filled out for both the ME and nasopharynx. The experimental procedure was carried out and bacteria were identified according to colony characteristics, morphological appearance, Gram-staining, and standard biochemical testing. Data obtained were analysed with SPSS version 16.0 and Epi Info 3.5.1 using the mean, standard deviation and chi-square results.
  
  
  RESULTS
  A total of 140 cases and 70 controls, were recruited. The Gram stain reaction of the ME aspirates were positive in 28.6% and negative in 71.4% of cases. Nasopharyngeal swabs revealed 64.3% Gram positive and 35.7% negative organisms. Overall, there was no relationship between the ME and nasopharyngeal isolates amongst cases, with aPvalue of 0.000. However, there was a relationship amongst the isolate from the nasopharynx of cases and controls, with the exception ofKlebsiella pneumoniae, atP< 0.009.
  
  
  CONCLUSIONS
  There was no relationship amongst the bacterial isolate from the ME and nasopharyngeal specimen of patients with otitis media.
authors.affiliation: Department of Ear, Nose and Throat Surgery, University of Ilorin/UITH, PMB 1515, Ilorin, Nigeria.
authors.forename: Olushola Abdulrahman
authors.initials: OA
authors.lastname: Afolabi
authors.affiliation: Department of Ear, Nose and Throat Surgery, University of Ilorin/UITH, PMB 1515, Ilorin, Nigeria.
authors.forename: Foluwasayo Emmanuel
authors.initials: FE
authors.lastname: Ologe
authors.affiliation: Department of Microbiology, University of Ilorin/UITH, PMB 1515 Ilorin, Nigeria.
authors.forename: Charles
authors.initials: C
authors.lastname: Nwabuisi
authors.affiliation: Department of Epidemiology and Community Health, University of Ilorin/UITH, PMB 1515, Ilorin, Nigeria.
authors.forename: Adekunle Ganiyu
authors.initials: AG
authors.lastname: Salaudeen
authors.affiliation: Family Medicine Department;UITH, PMB 1459, Ilorin, Nigeria.
authors.forename: Olalekan Tajudeen
authors.initials: OT
authors.lastname: Ajiboye
authors.affiliation: Ear, Nose and Throat Surgery Department, University of Lagos/LUTH, PMB, 56, Akoka, Yaba, Lagos, Nigeria.
authors.forename: Clement Chukwuemeka
authors.initials: CC
authors.lastname: Nwawolo
keywords: aspirate; chronic otitis media; correlation; microbiology; middle ear; nasopharyngeal swab; relationship