title: Environmental Occurrence and Antibiotic Susceptibility Profile of Listeria monocytogenes at a Slaughterhouse Raw Processing Plant in Romania.
abstract: This survey was conducted to investigate the occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates in the environment of a pig slaughterhouse raw processing plant complex in western Romania. A total of 97 environmental samples from food contact (n = 60) and nonfood contact (n = 37) surfaces were examined with standard methods. The susceptibility of the isolates to 17 antimicrobial agents was determined with the VITEK 2 automated system. Overall, 25 (25.8%) samples were positive for L. monocytogenes , with recovery rates of 23.3% (n = 14) and 29.7% (n = 11) for food contact and nonfood contact surfaces, respectively. All 25 tested isolates were resistant to benzylpenicillin, imipenem, and fusidic acid. Resistance was observed to oxacillin (23 isolates; 92%), fosfomycin (23 isolates; 92%), clindamycin (22 isolates; 88%), rifampin (14 isolates; 56%), trimethoprim-sulfamethoxazole (12 isolates; 48%), tetracycline (11 isolates; 44%), and ciprofloxacin (1 isolate; 4%). No resistance was found to seven tested agents: gentamicin, moxifloxacin, erythromycin, linezolid, teicoplanin, vancomycin, and tigecycline. All tested L. monocytogenes isolates were resistant to four to nine antimicrobial agents. These results indicate a high prevalence of multidrug-resistant L. monocytogenes isolates in this pork-processing environment, providing baseline information for hygienists and public health specialists.
authors.affiliation: Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine Timişoara, Banat University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timişoara, Calea Aradului 119, 300645 Timişoara, Romania.
authors.forename: Claudia
authors.initials: C
authors.lastname: Sala
authors.affiliation: Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine Timişoara, Banat University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timişoara, Calea Aradului 119, 300645 Timişoara, Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Morar
authors.affiliation: Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine Timişoara, Banat University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timişoara, Calea Aradului 119, 300645 Timişoara, Romania.
authors.forename: Emil
authors.initials: E
authors.lastname: Tîrziu
authors.affiliation: Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine Timişoara, Banat University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timişoara, Calea Aradului 119, 300645 Timişoara, Romania.
authors.forename: Ileana
authors.initials: I
authors.lastname: Nichita
authors.affiliation: Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine Timişoara, Banat University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timişoara, Calea Aradului 119, 300645 Timişoara, Romania.
authors.forename: Mirela
authors.initials: M
authors.lastname: Imre
authors.affiliation: Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine Timişoara, Banat University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timişoara, Calea Aradului 119, 300645 Timişoara, Romania.
authors.forename: Kálmán
authors.initials: K
authors.lastname: Imre
mesh_terms: D000003:Abattoirs; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D000069466:Red Meat; D012383:Romania; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antimicrobial resistance; Listeria; Pig meat; Slaughter