title: Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through Combined Field Tests, Quantitative Real-Time PCR, and Proteome Analysis.
abstract: Diseases caused by southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) considerably decrease grain yield. Therefore, determining rice cultivars with high resistance to SRBSDV and RRSV is necessary. In this study, rice cultivars with high resistance to SRBSDV and RRSV were evaluated through field trials in Shidian and Mangshi county, Yunnan province, China. SYBR Green I-based quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to quantitatively detect virus gene expression levels in different rice varieties. The following parameters were applied to evaluate rice resistance: acre yield (A.Y.), incidence of infected plants (I.I.P.), virus load (V.L.), disease index (D.I.), and insect quantity (I.Q.) per 100 clusters. Zhongzheyou1 (Z1) and Liangyou2186 (L2186) were considered the most suitable varieties with integrated higher A.Y., lower I.I.P., V.L., D.I. and I.Q.
  
  
  
  In order to investigate the mechanism of rice resistance, comparative label-free shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomic approaches were applied to comprehensively describe the proteomics of rice varieties' SRBSDV tolerance. Systemic acquired resistance (SAR)-related proteins in Z1 and L2186 may result in the superior resistance of these varieties compared with Fengyouxiangzhan (FYXZ).
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. wzc.4884@163.com.|College of Pharmacy, Guizhou University, Guiyang 550025, China. wzc.4884@163.com.
authors.forename: Zhenchao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science, Guizhou University, Guiyang 550025, China. yuji570@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. linhong_j@126.com.
authors.forename: Linhong
authors.initials: L
authors.lastname: Jin
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. wangwenli0208@163.com.
authors.forename: Wenli
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. fs1989430@gmail.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. ranlonglu@163.com.
authors.forename: Longlu
authors.initials: L
authors.lastname: Ran
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. xiangyangli83@163.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. gychenzhuo@aliyun.com.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: National Agricultural Extension Service Centre, Beijing 100026, China. guorong@agri.gov.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Guo
authors.affiliation: Shidian Plant Protection Station, Shidian 678200, China. yongtianwei2016@163.com.
authors.forename: Yongtian
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Shidian Plant Protection Station, Shidian 678200, China. sdyzc@163.com.
authors.forename: Zhongcheng
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Mangshi Plant Protection & Quarantine Station, Mangshi 678400, China. enlongliu2016@163.com.
authors.forename: Enlong
authors.initials: E
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. dyhu@gzu.edu.cn.
authors.forename: Deyu
authors.initials: D
authors.lastname: Hu
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. basong@gzu.edu.cn.
authors.forename: Baoan
authors.initials: B
authors.lastname: Song
mesh_terms: D002681:China; D060467:Disease Resistance; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification*; D020543:Proteome / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012087:Reoviridae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D020543:Proteome
keywords: field tests; label-free shotgun LC-MS/MS proteomics; quantitative real-time polymerase chain reaction; resistance properties; rice ragged stunt virus; southern rice black-streaked dwarf virus