title: Mesorhizobium delmotii and Mesorhizobium prunaredense are two new species containing rhizobial strains within the symbiovar anthyllidis.
abstract: Eight mesorhizobial symbiotic strains isolated from Anthyllis vulneraria root-nodules were studied and compared taxonomically with defined Mesorhizobium species. All strains presented identical 16S rDNA sequences but can be differentiated by multilocus sequence analysis of housekeeping genes (recA, atpD, glnII and dnaK). Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analyses separate these strains in two groups and a separate strain. Levels of DNA-DNA relatedness were less than 55% between representative strains and their closest Mesorhizobium reference relatives. The two groups containing four and three strains, respectively, originating from border mine and non-mining areas in Cévennes, were further phenotypically characterized. Groupings were further supported by average nucleotide identity values based on genome sequencing, which ranged from 80 to 92% with their close relatives and with each other, confirming these groups represent new Mesorhizobium species. Therefore, two novel species Mesorhizobium delmotii sp. nov. (type strain STM4623T=LMG 29640T=CFBP 8436T) and Mesorhizobium prunaredense sp. nov. (type strain STM4891T=LMG 29641T=CFBP 8437T) are proposed. Type strains of the two proposed species share accessory common nodulation genes within the new symbiovar anthyllidis as found in the Mesorhizobium metallidurans type strain.
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes CIRAD/INRA/IRD/Montpellier SupAgro/Université de Montpellier, TA A-82/J, 34398 Montpellier, France. Electronic address: roba392@gmail.com.
authors.forename: Roba
authors.initials: R
authors.lastname: Mohamad
authors.affiliation: Laboratory of Microbiology, Dept. Biochemistry and Microbiology, Ghent University, Ghent, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Willems
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes CIRAD/INRA/IRD/Montpellier SupAgro/Université de Montpellier, TA A-82/J, 34398 Montpellier, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Le Quéré
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes CIRAD/INRA/IRD/Montpellier SupAgro/Université de Montpellier, TA A-82/J, 34398 Montpellier, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Maynaud
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes CIRAD/INRA/IRD/Montpellier SupAgro/Université de Montpellier, TA A-82/J, 34398 Montpellier, France.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Pervent
authors.affiliation: Plateforme MGX, c/o Institut de Genomique Fonctionnelle, Montpellier, 141 rue de la Cardonille, 34 094 Montpellier Cedex, France.
authors.forename: Maurine
authors.initials: M
authors.lastname: Bonabaud
authors.affiliation: Plateforme MGX, c/o Institut de Genomique Fonctionnelle, Montpellier, 141 rue de la Cardonille, 34 094 Montpellier Cedex, France.
authors.forename: Emeric
authors.initials: E
authors.lastname: Dubois
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes CIRAD/INRA/IRD/Montpellier SupAgro/Université de Montpellier, TA A-82/J, 34398 Montpellier, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Cleyet-Marel
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes CIRAD/INRA/IRD/Montpellier SupAgro/Université de Montpellier, TA A-82/J, 34398 Montpellier, France. Electronic address: brigitte.brunel@supagro.fr.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Brunel
mesh_terms: D001482:Base Composition; D007887:Fabaceae / Q000382:microbiology*; D016680:Genome, Bacterial; D013058:Mass Spectrometry; D059128:Mesorhizobium / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Anthyllis vulneraria; Average nucleotide identity; Mass spectrometry; Mesorhizobium; Multilocus sequence analysis; Symbiosis