title: Draft genome sequence of Bradyrhizobium manausense strain BR 3351T, an effective symbiont isolated from Amazon rainforest.
abstract: The strain BR 3351T(Bradyrhizobium manausense) was obtained from nodules of cowpea (Vigna unguiculata L. Walp) growing in soil collected from Amazon rainforest. Furthermore, it was observed that the strain has high capacity to fix nitrogen symbiotically in symbioses with cowpea. We report here the draft genome sequence of strain BR 3351T. The information presented will be important for comparative analysis of nodulation and nitrogen fixation for diazotrophic bacteria. A draft genome with 9,145,311bp and 62.9% of GC content was assembled in 127 scaffolds using 100bp pair-end Illumina MiSeq system. The RAST annotation identified 8603 coding sequences, 51 RNAs genes, classified in 504 subsystems.
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Brazil.
authors.forename: Jean Luiz
authors.initials: JL
authors.lastname: Simões-Araújo
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Brazil.
authors.forename: Norma Gouvêa
authors.initials: NG
authors.lastname: Rumjanek
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Brazil.
authors.forename: Gustavo Ribeiro
authors.initials: GR
authors.lastname: Xavier
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Brazil. Electronic address: jerri.zilli@embrapa.br.
authors.forename: Jerri Édson
authors.initials: JÉ
authors.lastname: Zilli
mesh_terms: D001482:Base Composition; D020369:Bradyrhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001938:Brazil; D016680:Genome, Bacterial; D065947:Rainforest; D053204:Root Nodules, Plant / Q000382:microbiology; D013559:Symbiosis; D000070658:Vigna / Q000382:microbiology*
keywords: Amazon forest; Biological nitrogen fixation; Cowpea; Next generation sequencing; Nodulation