title: Investigation of the fungal community structures of imported wheat using high-throughput sequencing technology.
abstract: This study introduced the application of high-throughput sequencing techniques to the investigation of microbial diversity in the field of plant quarantine. It examined the microbial diversity of wheat imported into China, and established a bioinformatics database of wheat pathogens based on high-throughput sequencing results. This study analyzed the nuclear ribosomal internal transcribed spacer (ITS) region of fungi through Illumina Miseq sequencing to investigate the fungal communities of both seeds and sieve-through. A total of 758,129 fungal ITS sequences were obtained from ten samples collected from five batches of wheat imported from the USA. These sequences were classified into 2 different phyla, 15 classes, 33 orders, 41 families, or 78 genera, suggesting a high fungal diversity across samples. Apairwise analysis revealed that the diversity of the fungal community in the sieve-through is significantly higher than those in the seeds. Taxonomic analysis showed that at the class level, Dothideomycetes dominated in the seeds and Sordariomycetes dominated in the sieve-through. In all, this study revealed the fungal community composition in the seeds and sieve-through of the wheat, and identified key differences in the fungal community between the seeds and sieve-through.
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.
authors.forename: Yaqian
authors.identifier: http://orcid.org/0000-0002-1522-3800
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.|Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine, Shenzhen, China.|Kunming University of Science and Technology, Kunming, China.
authors.forename: Yinghui
authors.identifier: http://orcid.org/0000-0003-1246-1800
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.|Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine, Shenzhen, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.|Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine, Shenzhen, China.
authors.forename: Guiming
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.|Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine, Shenzhen, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Gao
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.|Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine, Shenzhen, China.
authors.forename: Caiyu
authors.initials: C
authors.lastname: Xiang
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.|Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine, Shenzhen, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Feng
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.|Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests, Shenzhen Scientific Academy of Inspection and Quarantine, Shenzhen, China.
authors.forename: Dingfeng
authors.initials: D
authors.lastname: Lou
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
mesh_terms: D044822:Biodiversity; D002681:China; D003132:Commerce; D019295:Computational Biology; D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D005658:Fungi / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D007391:International Cooperation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D014908:Triticum / Q000382:microbiology*; D014481:United States
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic