title: Development and characterization of mouse monoclonal antibodies reactive with chicken CXCLi2.
abstract: Interleukin-8(IL-8)/CXCL8 is a CXC-family chemokine that attracts lymphocytes to sites of tissue damage and plays a role in the inflammatory response and wound healing. Chicken chemotactic and angiogenic factor was referred to as chCXCLi2 and has been studied as one of human CXCL8 homologue for more than 20 years. However, no monoclonal antibodies (mAbs) that specifically detect chCXCLi2 have been developed. Here, we developed and characterized mouse mAbs against chCXCLi2 to define its immunological properties. Two mouse mAbs against chCXCLi2 were generated and confirmed to display specific binding with not only recombinants, but endogenous chCXCLi2 by Western blot analysis, ELISA, and immunocytochemistry. Inhibition of chCXCLi2-induced chemotactic activity on peripheral blood lymphocytes, proliferation of chicken macrophage cells and expression of alpha smooth-muscle actin in chicken embryonic fibroblast cells by antibodies indicate that these antibodies are capable of blocking chCXCLi2 bioactivity. These chCXCLi2 mAbs will be useful reagents for future investigations of inflammation in poultry.
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, ARS, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: Woo H
authors.initials: WH
authors.lastname: Kim
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, ARS, U.S. Department of Agriculture, Beltsville, MD 20705, USA. Electronic address: Hyun.Lillehoj@ars.usda.gov.
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, ARS, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: Yeaseul
authors.initials: Y
authors.lastname: Lim
authors.affiliation: College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University, Jinju 52828, South Korea.
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
authors.affiliation: Kingfisher Biotech Inc, St. Paul, MN 55144, USA.
authors.forename: Yvonne B
authors.initials: YB
authors.lastname: Sullivan
authors.affiliation: Kingfisher Biotech Inc, St. Paul, MN 55144, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Kakach
authors.affiliation: Kingfisher Biotech Inc, St. Paul, MN 55144, USA.
authors.forename: Joanna W
authors.initials: JW
authors.lastname: LaBresh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity / Q000276:immunology; D030161:Avian Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D002645:Chickens / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D017386:Sequence Homology, Amino Acid
chemical_list: D000911:Antibodies, Monoclonal; D030161:Avian Proteins; D016209:Interleukin-8; D011994:Recombinant Proteins
keywords: CXCL8; CXCLi2; Chicken; Interleukin-8; Monoclonal antibody