title: Bioinformatic prediction of G protein-coupled receptor encoding sequences from the transcriptome of the foreleg, including the Haller's organ, of the cattle tick, Rhipicephalus australis.
abstract: The cattle tick of Australia, Rhipicephalus australis, is a vector for microbial parasites that cause serious bovine diseases. The Haller's organ, located in the tick's forelegs, is crucial for host detection and mating. To facilitate the development of new technologies for better control of this agricultural pest, we aimed to sequence and annotate the transcriptome of the R. australis forelegs and associated tissues, including the Haller's organ. As G protein-coupled receptors (GPCRs) are an important family of eukaryotic proteins studied as pharmaceutical targets in humans, we prioritized the identification and classification of the GPCRs expressed in the foreleg tissues. The two forelegs from adult R. australis were excised, RNA extracted, and pyrosequenced with 454 technology. Reads were assembled into unigenes and annotated by sequence similarity. Python scripts were written to find open reading frames (ORFs) from each unigene. These ORFs were analyzed by different GPCR prediction approaches based on sequence alignments, support vector machines, hidden Markov models, and principal component analysis. GPCRs consistently predicted by multiple methods were further studied by phylogenetic analysis and 3D homology modeling. From 4,782 assembled unigenes, 40,907 possible ORFs were predicted. Using Blastp, Pfam, GPCRpred, TMHMM, and PCA-GPCR, a basic set of 46 GPCR candidates were compiled and a phylogenetic tree was constructed. With further screening of tertiary structures predicted by RaptorX, 6 likely GPCRs emerged and the strongest candidate was classified by PCA-GPCR to be a GABAB receptor.
authors.affiliation: The University of Texas at El Paso, Bioinformatics Program, El Paso, Texas, United States of America.
authors.forename: Sergio
authors.initials: S
authors.lastname: Munoz
authors.affiliation: USDA-ARS, Knipling Bushland US Livestock Insect Research Laboratory, Kerrville, Texas, United States of America.
authors.forename: Felix D
authors.initials: FD
authors.lastname: Guerrero
authors.affiliation: The University of Texas at El Paso, Bioinformatics Program, El Paso, Texas, United States of America.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Kellogg
authors.affiliation: USDA-ARS, Knipling Bushland US Livestock Insect Research Laboratory, Kerrville, Texas, United States of America.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Heekin
authors.affiliation: The University of Texas at El Paso, Bioinformatics Program, El Paso, Texas, United States of America.
authors.forename: Ming-Ying
authors.identifier: http://orcid.org/0000-0003-2035-1214
authors.initials: MY
authors.lastname: Leung
mesh_terms: D000818:Animals; D002417:Cattle / Q000469:parasitology*; D005260:Female; D023281:Genomics / Q000379:methods; D008297:Male; D016366:Open Reading Frames; D010802:Phylogeny; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D048494:Rhipicephalus / Q000235:genetics*; D059467:Transcriptome
chemical_list: D043562:Receptors, G-Protein-Coupled; D012313:RNA