title: Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding.
abstract: The olive fruit fly, Bactrocera oleae, is the most destructive pest of olive orchards worldwide. The monophagous larva has the unique capability of feeding on olive mesocarp, coping with high levels of phenolic compounds and utilizing non-hydrolyzed proteins present, particularly in the unripe, green olives. On the molecular level, the interaction between B. oleae and olives has not been investigated as yet. Nevertheless, it has been associated with the gut obligate symbiotic bacterium Candidatus Erwinia dacicola. Here, we used a B.oleae microarray to analyze the gene expression of larvae during their development in artificial diet, unripe (green) and ripe (black) olives. The expression profiles of Ca. E. dacicola were analyzed in parallel, using the Illumina platform. Several genes were found overexpressed in the olive fly larvae when feeding in green olives. Among these, a number of genes encoding detoxification and digestive enzymes, indicating a potential association with the ability of B. oleae to cope with green olives. In addition, a number of biological processes seem to be activated in Ca. E. dacicola during the development of larvae in olives, with the most notable being the activation of amino-acid metabolism.
authors.affiliation: Department of Biology, University of Crete, 71409 Heraklion, Greece.|Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam (UvA), Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Nena
authors.initials: N
authors.lastname: Pavlidi
authors.affiliation: Department of Biology, University of Crete, 71409 Heraklion, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Gioti
authors.affiliation: Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam (UvA), Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Nicky
authors.initials: N
authors.lastname: Wybouw
authors.affiliation: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors.forename: Wannes
authors.initials: W
authors.lastname: Dermauw
authors.affiliation: Department of Entomology, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Michael
authors.initials: M
authors.lastname: Ben-Yosef
authors.affiliation: Department of Entomology, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Boaz
authors.initials: B
authors.lastname: Yuval
authors.affiliation: Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Edouard
authors.initials: E
authors.lastname: Jurkevich
authors.affiliation: Department of Biology, University of Crete, 71409 Heraklion, Greece.|Institute of Molecular Biology &Biotechnology, Foundation for Research &Technology Hellas, 100 N. Plastira Street, GR-700 13, Heraklion Crete, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Kampouraki
authors.affiliation: Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam (UvA), Science Park 904, 1098 XH Amsterdam, The Netherlands.|Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium.
authors.forename: Thomas
authors.initials: T
authors.lastname: Van Leeuwen
authors.affiliation: Institute of Molecular Biology &Biotechnology, Foundation for Research &Technology Hellas, 100 N. Plastira Street, GR-700 13, Heraklion Crete, Greece.|Laboratory of Pesticide Science, Department of Crop Science, Agricultural University of Athens, 11855-Athens, Greece.
authors.forename: John
authors.initials: J
authors.lastname: Vontas
mesh_terms: D000818:Animals; D004885:Erwinia / Q000235:genetics*; D005638:Fruit / Q000469:parasitology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D060434:Herbivory; D007814:Larva; D031658:Olea / Q000469:parasitology*; D015203:Reproducibility of Results; D013559:Symbiosis; D033621:Tephritidae / Q000235:genetics* / Q000382:microbiology*; D059467:Transcriptome