title: Proteome Characterization of Leaves in Common Bean.
abstract: Dry edible bean (Phaseolus vulgarisL.) is a globally relevant food crop. The bean genome was recently sequenced and annotated allowing for proteomics investigations aimed at characterization of leaf phenotypes important to agriculture. The objective of this study was to utilize a shotgun proteomics approach to characterize the leaf proteome and to identify protein abundance differences between two bean lines with known variation in their physiological resistance to biotic stresses. Overall, 640 proteins were confidently identified. Among these are proteins known to be involved in a variety of molecular functions including oxidoreductase activity, binding peroxidase activity, and hydrolase activity. Twenty nine proteins were found to significantly vary in abundance (p-value < 0.05) between the two bean lines, including proteins associated with biotic stress. To our knowledge, this work represents the first large scale shotgun proteomic analysis of beans and our results lay the groundwork for future studies designed to investigate the molecular mechanisms involved in pathogen resistance.
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, CO 80523, USA. fmr33@cornell.edu.|Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA. fmr33@cornell.edu.
authors.forename: Faith M
authors.initials: FM
authors.lastname: Robison
authors.affiliation: Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USA. Adam.Heuberger@colostate.edu.
authors.forename: Adam L
authors.initials: AL
authors.lastname: Heuberger
authors.affiliation: Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA. Mark.Brick@ColoState.EDU.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Brick
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, CO 80523, USA. jprenni@colostate.edu.|Department of Biochemistry, Colorado State University, Fort Collins, CO 80523, USA. jprenni@colostate.edu.
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Prenni
keywords: LC-MS; Phaseolus vulgaris; beans; leaves; pathogen resistance; plant defense; shotgun proteomics