title: The role of miR-17-92 cluster in the expression of tumor suppressor genes in unrestricted somatic stem cells.
abstract: The miR-17-92 cluster consisted of seven miRNAs (mir-17-5p, -17-3p, -18a, -19a, -20a, -19b-1, and -92a-1). Previous studies have shown this cluster has been over-expressed in several cancers. The aim of this study was to evaluate the over-expression impacts of miR-17-92 on stem cells. In the current work, the effect of miR-17-92 cluster which was cloned in Lentiviral vector has been investigated on unrestricted somatic stem cells (USSCs). Tumor suppressor genes (p53, p15, RBL1, SMAD2, SMAD4, and MAPK-1) expression, especially p53, was considerably reduced. These data show the potential of miR-17-92 for oncogenesis regulation in stem cells. In conclusion, the role of miR-17-92 in USSCs may provide a better understanding of its function in tumorigenesis and for the possible use in cell therapy of the anti-mir-17-92 cluster.
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Rezvan
authors.initials: R
authors.lastname: Tavakoli
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Vakilian
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Lida
authors.initials: L
authors.lastname: Langroudi
authors.affiliation: Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Arefian
authors.affiliation: Department of Clinical Biochemistry, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sahmani
authors.affiliation: Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Science, Qazvin, Iran. Electronic address: fjamshidiadegani@gmail.com.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Jamshidi-Adegani
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D002478:Cells, Cultured; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics; D005810:Multigene Family; D062085:RNA, Long Noncoding; D050720:Retinoblastoma-Like Protein p107 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051899:Smad2 Protein / Q000235:genetics; D051901:Smad4 Protein / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D050762:Cyclin-Dependent Kinase Inhibitor p15; C568997:MIR17HG, human; D035683:MicroRNAs; C493844:RBL1 protein, human; D062085:RNA, Long Noncoding; D050720:Retinoblastoma-Like Protein p107; C497782:SMAD2 protein, human; C497796:SMAD4 protein, human; D051899:Smad2 Protein; D051901:Smad4 Protein; D016159:Tumor Suppressor Protein p53; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1
keywords: Tumor suppressor gene; Tumorigenesis; miR-17-92 cluster; microRNAs