title: PDPN gene promotes the proliferation of immature Bovine Sertoli cells in vitro.
abstract: Podoplanin (PDPN) is a transmembrane receptor which is involved in various physiological and pathological processes, such as cell motility, invasion, tumor metastasis and blood vessels formation. Although there are reports on the involvement of PDPN in Sertoli cells in human and mice, the role of PDPN on the development of bovine Sertoli cells has not been reported. In the present study, Sertoli cells were isolated from 1-day-old bovine testes by two steps enzyme digestion method. Feulgen staining of satellite karyosomes and inhibin immunofluorescence staining suggested that the isolated immature Sertoli cells were very pure. Transfection with overexpression plasmid pBI-CMV3-PDPN and interference shRNA plasmid indicated that PDPN could significantly promote Sertoli cells cycle progression, cells proliferation and androgen-binding protein (ABP) production. Our results indicated that PDPN gene plays a significant role in the proliferation and maturation of bovine Sertoli cells.
authors.affiliation: Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100, China; College of Animal Science and Technology, Jilin Agriculture University, Xin Cheng Street 2888, Changchun, Jilin 130118, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Qin
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin 130062, China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Agriculture University, Xin Cheng Street 2888, Changchun, Jilin 130118, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Dong
authors.affiliation: College of Animal Science and Technology, Jilin Agriculture University, Xin Cheng Street 2888, Changchun, Jilin 130118, China.
authors.forename: Zijiao
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100, China. Electronic address: nongkeyuanzhang@163.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin 130062, China. Electronic address: zhzhao@jlu.edu.cn.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000727:Androgen-Binding Protein / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012708:Sertoli Cells / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D000727:Androgen-Binding Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger
keywords: PDPN; Proliferation; Sertoli cells