title: Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity.
abstract: A new synthetic biology engineering strategy integrating chemical reactivity sensing and small molecule induced protein dimerization has been developed to generate artificial Fe2+signaling circuitry to control tailored cellular events in mammalian cells. The dual function probe ABA-FE18 (Fe2+-sensing and protein dimerization) derived from ABA was developed and used to control gene activation, signal transduction, and cytoskeletal remodeling in response to Fe2+. This technology was utilized to design signal circuitry incorporating "AND" and "OR" biologic gates that enables mammalian cells to translate different combinations of Fe2+and H2O2signals into predefined biological outputs.
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.
authors.forename: Guihua
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.|Department of Medicinal Chemistry, College of Pharmaceutical Sciences, Soochow University , Suzhou 215123, P. R. China.
authors.forename: Huanqiu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.
authors.forename: Weimin
authors.initials: W
authors.lastname: Xuan
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.
authors.forename: Roushu
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.
authors.forename: Larisa E
authors.initials: LE
authors.lastname: Breden
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , Albuquerque, NM87131, United States.
authors.forename: Fu-Sen
authors.identifier: 0000-0002-1793-0824
authors.initials: FS
authors.lastname: Liang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002460:Cell Line; D006801:Humans; D007501:Iron / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D055503:Protein Multimerization / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D011993:Recombinant Fusion Proteins; D007501:Iron
keywords: abscisic acid; biologic gate; biosensor; cell signaling; chemically induced proximity; labile iron