title: Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function.
abstract: Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.
authors.affiliation: Metabolic Health Center, Division of Cardiology, Department of Medicine, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10021, USA.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Gómez-Banoy
authors.affiliation: Metabolic Health Center, Division of Cardiology, Department of Medicine, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10021, USA. jal2063@med.cornell.edu.
authors.forename: James C
authors.initials: JC
authors.lastname: Lo
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D004734:Energy Metabolism / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D051379:Mice; D014161:Transduction, Genetic; D019076:Transgenes; D014780:Viruses / Q000145:classification / Q000235:genetics*
keywords: AAV; Adeno-associated virus; Adenovirus; Adipocyte; Adipogenesis; Adipokine; Adipose tissue; Beige fat; Brown fat; Diabetes; Lentivirus; Metabolism; Obesity; Retrovirus; Transduction; Viral vectors; White fat