title: A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of ferritin and β2-microglobulin.
abstract: BACKGROUND
  Lymphocytic leukemia is a kind of primary malignant tumor of hematopoietic tissue. The aim was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of ferritin (FER) and β2-microglobulin (β2-MG) for the early screening and follow-up surveillance of lymphocytic leukemia.
  
  
  METHODS
  The sandwich immunoassay was used to detect the concentration of FER, and the competitive immunoassay was used to detect the concentration of β2-MG in serum. FER in serum was captured by anti- FER antibody immobilized on microtiter wells, and then banded together with another anti- FER labeled with europium(III) Eu3+chelate, followed by fluorescence measurement using time-resolved fluorometry (TRF). Sm3+labeled β2-MG and β2-MG samples were added to compete with a certain amount of anti-β2-MG antibody, followed by fluorescence measurement using TRF. The performance of this dual-label TRFIA was evaluated using the clinical blood and compared with the commercial assays.
  
  
  RESULTS
  The linear correlation coefficient (R2) of the FER and β2-MG standard curves were 0.9914 and 0.9927, respectively. The sensitivity for FER detection was 8 ng/mL (dynamic range 0-1000 ng/mL), the average recovery was 100.51%; The sensitivity for β2-MG detection was 1 ng/mL (dynamic range 0-1000 ng/mL), the average recovery was 101.02%. High correlation coefficients (R2) were obtained between the commercial assays (R2=.9966 for FER, and R2=.9897 for β2-MG).
  
  
  CONCLUSIONS
  The present dual-label TRFIA has high sensitivity, specificity, and accuracy in clinical sample analysis. It is an effective detection method for the early screening and follow-up surveillance of the acute and chronic lymphocytic leukemia.
authors.affiliation: Department of Hematology, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Hematology, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Hematology, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Rui-Ming
authors.initials: RM
authors.lastname: Ou
authors.affiliation: Department of Hematology, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Meng-Dong
authors.initials: MD
authors.lastname: Yao
authors.affiliation: Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: She
authors.affiliation: Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Hematology, The first hospital of Kashgar district of Xin Jiang, Xinjiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Hematology, The first hospital of Kashgar district of Xin Jiang, Xinjiang, China.
authors.forename: Aikebaier
authors.initials: A
authors.lastname: Abudureyimu
authors.affiliation: Department of Hematology, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Guangdong NO. 2 Provincial People's Hospital, Guangdong, Guangzhou, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
mesh_terms: D005293:Ferritins / Q000097:blood*; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D001613:beta 2-Microglobulin / Q000097:blood*
chemical_list: D001613:beta 2-Microglobulin; D005293:Ferritins
keywords: dual-label time-resolved fluorescence immunoassay; ferritin; lymphocytic leukemia; β2-microglobulin