title: Cardiolipin Supports Respiratory Enzymes in Plants in Different Ways.
abstract: In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown to affect abundances of the plant NADH-dehydrogenase complex and its association with dimeric cyctochrome c reductase. Using anArabidopsis thalianaknock-out mutant for the final enzyme of CL biosynthesis we here extend current knowledge on the dependence of plant respiration on CL. By correlating respiratory enzyme abundances with enzymatic capacities in mitochondria isolated from wild type, CL deficient and CL complemented heterotrophic cell culture lines a new picture of the participation of CL in plant respiration is emerging. Data indicate a loss of a general reduction of respiratory capacity in CL deficient mitochondria which cannot solely be attributed to decreased abundances or capacities of mitochondrial electron transfer protein complexes and supercomplexes. Instead, it most likely is the result of a loss of the mobile electron carrier cytochrome c. Furthermore, enzymes of the tricarboxylic acid cycle are found to have lower maximum activities in the mutant, including the succinate dehydrogenase complex. Interestingly, abundance of the latter is not altered, indicative of a direct impact of CL deficiency on the enzymatic capacity of this electron transfer chain protein complex.
authors.affiliation: Institute of Plant Genetics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Jakob
authors.initials: J
authors.lastname: Petereit
authors.affiliation: Graduate School of Arts and Sciences, The University of TokyoTokyo, Japan; Graduate School of Agricultural and Life Sciences, The University of TokyoTokyo, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Katayama
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Dortmund, Germany.
authors.forename: Christin
authors.initials: C
authors.lastname: Lorenz
authors.affiliation: Institute of Plant Genetics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Ewert
authors.affiliation: Institute of Plant Genetics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schertl
authors.affiliation: Institute of Biostatistics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kitsche
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo Tokyo, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Wada
authors.affiliation: Institute of Biology I, RWTH Aachen University Aachen, Germany.
authors.forename: Margrit
authors.initials: M
authors.lastname: Frentzen
authors.affiliation: Institute of Plant Genetics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Braun
authors.affiliation: Institute of Plant Genetics, Leibniz Universität Hannover Hannover, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Eubel
keywords: cardiolipin; cytochrome c; plant mitochondria; protein complexes; protein supercomplexes; respiration