title: Re-emergence of bovine haemorrhagic septicaemia in Hungary.
abstract: This paper reports an outbreak of haemorrhagic septicaemia caused by Pasteurella multocida B:2 in beef calves, a disease that has not been described in the Hungarian literature since 1943, and has not been reported to the World Organisation For Animal Health (OIE) since 1970. Acute haemorrhagic septicaemia was confirmed in beef calves on one small farm, and was suspected on two further nearby holdings with concomitant unexplained losses. The source of the infection could not be determined. Apart from a short duration of depression and loss of appetite, the affected calves developed characteristic distal limb oedema. Gross findings in two calves submitted for laboratory examinations included subcutaneous oedema and haemorrhages on serous membranes, and in one case severe pharyngeal lymph node enlargement was observed. Histological examinations revealed lesions characteristic of septicaemia. Moderate to large amounts of Pasteurella antigens were detected in all organs tested by immunohistochemistry. Two isolates of P. multocida (Pm240, Pm241) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. Both were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST64) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences , Budapest , Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Magyar
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences , Budapest , Hungary.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ujvári
authors.affiliation: Veterinary Diagnostic Directorate, Central Agriculture Office , Budapest , Hungary.
authors.forename: Levente
authors.initials: L
authors.lastname: Szeredi
authors.affiliation: Private Veterinarian, Szendehely , Hungary.
authors.forename: Norbert
authors.initials: N
authors.lastname: Virsinger
authors.affiliation: Department and Clinic for Production Animals, University of Veterinary Medicine , H-2225 Üllő , Hungary.|MTA-SZIE Large Animal Clinical Research Group , Üllő , Hungary.
authors.forename: Ervin
authors.initials: E
authors.lastname: Albert
authors.affiliation: Department and Clinic for Production Animals, University of Veterinary Medicine , H-2225 Üllő , Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Német
authors.affiliation: Department and Clinic for Production Animals, University of Veterinary Medicine , H-2225 Üllő , Hungary.
authors.forename: Edit
authors.initials: E
authors.lastname: Csuka
authors.affiliation: Department and Clinic for Production Animals, University of Veterinary Medicine , H-2225 Üllő , Hungary.
authors.forename: Imre
authors.initials: I
authors.lastname: Biksi
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D021821:Communicable Diseases, Emerging; D004196:Disease Outbreaks / Q000662:veterinary*; D006483:Hemorrhagic Septicemia / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006814:Hungary / Q000453:epidemiology; D016979:Pasteurella multocida / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny
keywords: Pasteurella multocida; beef cattle; capsular type B; haemorrhagic septicaemia; histopathology; immunohistochemistry; multilocus sequence typing