title: Accumulation of immunoglobulin G against Dermatophagoides farinae tropomyosin in dorsal root ganglia of NC/Nga mice with atopic dermatitis-like symptoms.
abstract: Atopic dermatitis (AD), a chronic inflammatory skin disease, manifests as intractable itch, but its underlying mechanisms are poorly understood. This study assessed the relationship between immunoglobulin G (IgG) and dorsal root ganglia (DRG) in NC/Nga mice, a model of AD that manifests AD-like symptoms including itch. Immunohistochemical analysis showed large amounts of IgG in DRG extracts of NC/Nga mice with AD-like dermatitis, with a large fraction of the IgG distributed in satellite glial cells of the DRG. Proteomic analysis showed that this IgG was reactive against tropomyosin of Dermatophagoides farinae. These findings indicate that the accumulation of anti-tropomyosin IgG in DRG of atopic NC/Nga mice may be associated with the pathogenesis of AD-like symptoms, including itch.
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan; Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Otsu
authors.affiliation: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Tominaga
authors.affiliation: Institute of Health and Sports Science & Medicine, Juntendo University, 1-1 Hirakagakuendai, Inzai, Chiba 270-1695, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Shigenaga
authors.affiliation: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Matsuda
authors.affiliation: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Takahashi
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: Institute of Health and Sports Science & Medicine, Juntendo University, 1-1 Hirakagakuendai, Inzai, Chiba 270-1695, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Naito
authors.affiliation: Juntendo University, School of Medicine, 1-1 Hirakagakuendai, Inzai, Chiba 270-1695, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Baba
authors.affiliation: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tomooka
authors.affiliation: Juntendo University Faculty of International Liberal Arts, 2-1-1, Bunkyoku, Hongo, Tokyo 113-8421, Japan. Electronic address: hyamaku@juntendo.ac.jp.
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Yamakura
authors.affiliation: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan; Department of Dermatology, Juntendo University, Urayasu Hospital, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan. Electronic address: ktakamor@juntendo.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Takamori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D060829:Arthropod Proteins / Q000276:immunology*; D015153:Blotting, Western; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism; D040001:Dermatophagoides farinae / Q000276:immunology*; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009457:Neuroglia / Q000276:immunology / Q000378:metabolism; D020543:Proteome / Q000276:immunology / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014335:Tropomyosin / Q000276:immunology*
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D007074:Immunoglobulin G; D020543:Proteome; D014335:Tropomyosin
keywords: Atopic dermatitis; Dorsal root ganglia; IgG; Itch; Tropomyosin