title: Colistin Resistance Genemcr-1and Its Variant in Escherichia coli Isolates from Chickens in China.
abstract: Themcr-1gene was detected in 5.11% (58/1136) ofEscherichia coliisolates of chicken origin from 13 provinces in China. A novelmcr-1variant, namedmcr-1.3, encoding an Ile-to-Val functional variant of MCR-1 was identified in a sequence type 155 (ST155) strain. Anmcr-1.3-containing IncI2 plasmid, pHeN867 (60,757 bp), was identified. The transfer of pHeN867 led to a 32-fold increase in the MIC of colistin in the recipient, exhibiting an effect on colistin resistance that was similar to that ofmcr-1.
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Yang
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Li
authors.affiliation: Microbiology and Metabolic Engineering of Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Yan-Xian
authors.initials: YX
authors.lastname: Yang
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: An-Yun
authors.initials: AY
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Bio-Resources and Eco-Environment, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Guo
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Bi-Hui
authors.initials: BH
authors.lastname: Liu
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Chang-Wei
authors.initials: CW
authors.lastname: Lei
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.affiliation: Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, China whongning@163.com.
authors.forename: Hong-Ning
authors.initials: HN
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology*; D002681:China; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000662:veterinary; D029968:Escherichia coli Proteins / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011201:Poultry Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C000614037:MCR-1 protein, E coli; D001618:beta-Lactamases; C000602253:beta-lactamase CTX-M, E coli; D003091:Colistin
keywords: E. coli; colistin resistance; mcr-1.3; plasmid