title: Acute Adaption to Oral or Intravenous Phosphate Requires Parathyroid Hormone.
abstract: Phosphate (Pi) homeostasis is regulated by renal, intestinal, and endocrine mechanisms through which Pi intake stimulates parathyroid hormone (PTH) and fibroblast growth factor-23 secretion, increasing phosphaturia. Mechanisms underlying the early adaptive phase and the role of the intestine, however, remain ill defined. We investigated mineral, endocrine, and renal responses during the first 4 hours after intravenous and intragastric Pi loading in rats. Intravenous Pi loading (0.5 mmol) caused a transient rise in plasma Pi levels and creatinine clearance and an increase in phosphaturia within 10 minutes. Plasma calcium levels fell and PTH levels increased within 10 minutes and remained low or high, respectively. Fibroblast growth factor-23, 1,25-(OH)2-vitamin D3, and insulin concentrations did not respond, but plasma dopamine levels increased by 4 hours. In comparison, gastric Pi loading elicited similar but delayed phosphaturia and endocrine responses but did not affect plasma mineral levels. Either intravenous or gastric loading led to decreased expression and activity of renal Pi transporters after 4 hours. In parathyroidectomized rats, however, only intravenous Pi loading caused phosphaturia, which was blunted and transient compared with that in intact rats. Intravenous but not gastric Pi loading in parathyroidectomized rats also led to higher creatinine clearance and lower plasma calcium levels but did not reduce the expression or activity of Pi transporters. This evidence suggests that an intravenous or intestinal Pi bolus causes rapid phosphaturia through mechanisms requiring PTH and downregulation of renal Pi transporters but does not support a role of the intestine in stimulating renal clearance of Pi.
authors.affiliation: Institute of Physiology, University of Zurich, Zurich, Switzerland; and National Centre for Competence in Research, Zurich, Switzerland.
authors.forename: Linto
authors.initials: L
authors.lastname: Thomas
authors.affiliation: Institute of Physiology, University of Zurich, Zurich, Switzerland; and National Centre for Competence in Research, Zurich, Switzerland.
authors.forename: Carla
authors.initials: C
authors.lastname: Bettoni
authors.affiliation: Institute of Physiology, University of Zurich, Zurich, Switzerland; and National Centre for Competence in Research, Zurich, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Knöpfel
authors.affiliation: Institute of Physiology, University of Zurich, Zurich, Switzerland; and National Centre for Competence in Research, Zurich, Switzerland.
authors.forename: Nati
authors.initials: N
authors.lastname: Hernando
authors.affiliation: Institute of Physiology, University of Zurich, Zurich, Switzerland; and National Centre for Competence in Research, Zurich, Switzerland.
authors.forename: Jürg
authors.initials: J
authors.lastname: Biber
authors.affiliation: Institute of Physiology, University of Zurich, Zurich, Switzerland; and National Centre for Competence in Research, Zurich, Switzerland Wagnerca@access.uzh.ch.
authors.forename: Carsten A
authors.initials: CA
authors.lastname: Wagner
mesh_terms: D000222:Adaptation, Physiological; D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D007015:Hypophosphatemia, Familial / Q000209:etiology; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D010281:Parathyroid Hormone / Q000502:physiology*; D010710:Phosphates / Q000008:administration & dosage* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010281:Parathyroid Hormone; D010710:Phosphates
keywords: intestine; parathyroid hormone; phosphate uptake; phosphate-sensing