title: Depletion of abundant human serum proteins by per se imprinted cryogels based on sample heterogeneity.
abstract: Macroporous cryogels were prepared and used to deplete abundant proteins. It was accomplished based on the sample heterogeneity rather than any exogenous assistance. Human serum was added in monomer solutions to synthesize molecularly imprinted polymers; therein some abundant proteins were imprinted in the polyacrylamide cryogels. Meanwhile the rare components remained aqueous. Chromatography and electrophoresis showed that albumin, serotransferrin, and most globulins were depleted by columns packed with the molecularly imprinted polymers. After the depletion, lower abundance proteins were revealed by SDS-PAGE, peptide fingerprint analysis, and identified by MALDI-TOF-MS. This is an example that a "per se imprint" protocol enables to gradually dimidiate proteomes, simplify sample complexities, and facilitate further proteome profiling or biomarker discovery.
authors.affiliation: School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China.
authors.forename: Ya-Ru
authors.initials: YR
authors.lastname: Liu
authors.affiliation: School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Tian
authors.affiliation: School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Yan
authors.affiliation: School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China.
authors.forename: Wei-Qin
authors.initials: WQ
authors.lastname: Cao
authors.affiliation: Testing Centre, Yangzhou University, P. R., China.
authors.forename: Yu-Yang
authors.initials: YY
authors.lastname: Wang
mesh_terms: D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D059985:Cryogels / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D059985:Cryogels; D011108:Polymers
keywords: Depletion; Human serum; Molecularly imprinted polymer; Protein