title: Deriving cells expressing markers of female germ cells from premature ovarian failure patient-specific induced pluripotent stem cells.
abstract: We proposed a two-step protocol for deriving cells expressing markers of female germ cells (FGCs) from premature ovarian failure patient-specific induced pluripotent stem cells (POF-iPSCs).
  
  
  
  We cultured POF-iPSCs in suspension and pretreated them with TGFβ-1 (1 ng/ml) for 2 days and continued with both TGFβ-1 and BMP4 (50 ng/ml) for 5 more days. Then changed to media containing retinoic acid (1 μM) and 5% follicular fluid for another 7 days. Expression of markers of different stages of FGCs were detected.
  
  
  
  c-KIT, STELLA/DPPA3, VASA/DDX4, SCP3, GDF9 and ZP3 were positively detected and statistically significant different when compared with control groups.
  
  
  
  Our in vitro system was beneficial for POF-iPSCs differentiated cells to express STELLA, VASA and SCP3, which were the markers of meiosis initiation of FGCs.
authors.affiliation: Center for Reproductive Medicine, The Third Affiliated Hospital, Sun Yat-sen University, 6 East Longkou Road, Guangzhou, China.|Center for Reproductive Medicine, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Center for Reproductive Medicine, The Third Affiliated Hospital, Sun Yat-sen University, 6 East Longkou Road, Guangzhou, China.
authors.forename: Wen
authors.initials: W
authors.lastname: He
authors.affiliation: Center for Reproductive Medicine, The Third Affiliated Hospital, Sun Yat-sen University, 6 East Longkou Road, Guangzhou, China.|Department of Reproductive Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China.
authors.forename: Manbo
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Center for Reproductive Medicine, The Third Affiliated Hospital, Sun Yat-sen University, 6 East Longkou Road, Guangzhou, China.|Memorial hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Minhui
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Center for Reproductive Medicine, The Third Affiliated Hospital, Sun Yat-sen University, 6 East Longkou Road, Guangzhou, China.
authors.forename: Liuhong
authors.initials: L
authors.lastname: Cai
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D059247:Fertility Preservation; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D016649:Primary Ovarian Insufficiency / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers
keywords: embryoid body; female germ cells; follicular fluid; induced pluripotent stem cells; meiosis; peripheral blood mononuclear cells; premature ovarian failure