title: Collapsed state of polyglutamic acid results in amyloid spherulite formation.
abstract: Self-assembly of proteins and peptides into amyloid fibrils involves multiple distinct intermediates and late-stage fibrillar polymorphs. Understanding the conditions and mechanisms that promote the formation of one type of intermediate and polymorph over the other represents a fundamental challenge. Answers to this question are also of immediate biomedical relevance since different amyloid aggregate species have been shown to have distinct pathogenic potencies. One amyloid polymorph that has received comparatively little attention are amyloid spherulites. Here we report that self-assembly of the intrinsically disordered polymer poly(L-glutamic) acid (PLE) can generate amyloid spherulites. We characterize spherulite growth kinetics, as well as the morphological, optical and tinctorial features of this amyloid polymorph previously unreported for PLE. We find that PLE spherulites share both tinctorial and structural characteristics with their amyloid fibril counterparts. Differences in PLE's molecular weight, polydispersity or chemistry could not explain the selective propensity toward either fibril or spherulite formation. Instead, we provide evidence that PLE polymers can exist in either a collapsed globule or an extended random coil conformation. The collapsed globule consistently produces spherulites while the extended coil assembles into disordered fibril bundles. This results suggests that these 2 PLE conformers directly affect the morphology of the resulting macroscopic amyloid assembly.
authors.affiliation: Department of Physics; University of South Florida ; Tampa, FL USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Stehli
authors.affiliation: Department of Physics; University of South Florida ; Tampa, FL USA.
authors.forename: Mentor
authors.initials: M
authors.lastname: Mulaj
authors.affiliation: Department of Physics; University of South Florida ; Tampa, FL USA.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Miti
authors.affiliation: Department of Physics; University of South Florida ; Tampa, FL USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Traina
authors.affiliation: Department of Physics; University of South Florida ; Tampa, FL USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Foley
authors.affiliation: Department of Physics; University of South Florida ; Tampa, FL USA.
authors.forename: Martin
authors.initials: M
authors.lastname: Muschol
keywords: amyloid; birefringence; polyglutamic acid; polymer conformation; polymorphism; spectroscopy