title: Differential Maturation of miR-17 ~ 92 Cluster Members in Human Cancer Cell Lines.
abstract: While some microRNAs are transcribed from a specific promoter, at least one third of human miRNA genes are clustered, wherein multiple miRNA genes are generated from a single primary transcript such as miR-17 ~ 92 cluster. Although six members of the cluster are generated from a single transcript, the mature level of each member may be diverse in various cell types. Here, we attempt to monitor the mature level of miR-17, miR-92a, and miR-20a from miR-17 ~ 92 cluster in blood (HL60 (human promyelocytic leukemia cells) and Jurkat) and breast (MDA-MB-231 and MCF-7) cancer cell lines. Interestingly, different mature levels of the miRNAs were observed in each cell line. While miR-20 was highly matured in HL60 and MDA-MB-231 cell lines, higher mature level of miR-92a was observed in Jurkat cell line compared to that of miR-20 and miR-17. Further, the mature level of miRNAs was also measured in normal and cancer cell lines. Although the mature level of miR-17 and miR-92a increased in HL60 and Jurkat cell lines, miR-20 expression showed an almost identical level in blood cancer cell lines compared to controls. Conversely, miR-20 mature level significantly increased in breast cancer cell lines whereas the expression level of miR-92a was comparable in MDA-MB-231, MCF-7, and MCF-10A cell lines.
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Science, Tabriz, Iran.|Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Mozhgan
authors.initials: M
authors.lastname: Abasi
authors.affiliation: Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran.|Department of Cell, Molecular, and Structural Biology, Miami University, Oxford, OH, USA.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Kohram
authors.affiliation: Medical Laboratory Sciences Department, Faculty of Para-Medicine, Alborz University of Medical Sciences, Karaj, Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Fallah
authors.affiliation: Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Arashkia
authors.affiliation: Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Science, Tabriz, Iran. zarghamin@tbzmed.ac.ir.|Drug Applied Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. zarghamin@tbzmed.ac.ir.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
authors.affiliation: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hghanbarian@sbmu.ac.ir.|Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hghanbarian@sbmu.ac.ir.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ghanbarian
mesh_terms: D000293:Adolescent; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*
chemical_list: C524941:MIRN17 microRNA, human; C534451:MIRN20a microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs
keywords: Cancer cell lines; Stem cell; miR-17 ~ 92 cluster; microRNA maturation