title: Isolation of High-Molecular-Weight DNA from Mammalian Tissues Using Proteinase K and Phenol.
abstract: This procedure is the method of choice for purification of genomic DNA from mammalian tissues when large amounts of DNA are required, for example, for Southern blotting.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Green
authors.forename: Joseph
authors.initials: J
authors.lastname: Sambrook
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D019286:Endopeptidase K; D008322:Mammals / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D008970:Molecular Weight; D019800:Phenol
chemical_list: D019800:Phenol; D004247:DNA; D019286:Endopeptidase K