title: Impact of tillage practices on soil bacterial diversity and composition under the tobacco-rice rotation in China.
abstract: Tobacco-rice rotation is a common farming system in south China, and many tillage practices such as straw mulching, dolomite dust, and quicklime application have been adopted to improve crop production. These agricultural management practices alter soil physical and chemical properties and affect microbial life environment and community composition. In this research, six tillage practices including no tobacco and rice straw mulching (CK), tobacco and rice straw mulching (TrSr), rice straw returning fire (TrSc), tobacco and rice straw mulching with dolomite dust (TSD), rice straw returning fire and quicklime (TSQ), and rice straw returning fire, quicklime and reduced fertilizer (TSQf) were conducted to detect changes in soil bacterial diversity and composition using Illumina sequencing. The results showed that the total number of operational taxonomic units (OTUs) from the six treatments was 2030, and the number of mutual OTUs among all samples was 550. The TrSc treatment had the highest diversity and richness, while TSQf had the lowest. Soil physio-chemical properties and microbial diversity can influence each other. Proteobacteria and Actinobacteria had the greatest proportion in all treatments. The abundance of Nitrospirae was the highest in the TrSc treatment. The TSQf treatment had the highest abundance of Firmicutes. The abundance of Nitrospira in the TrSc treatment was 2.29-fold over CK. Streptomyces affiliated with Firmicutes improved by 37.33% in TSQf compared to TSQ. TSQf treatment was considered to be the most important factor in determining the relative abundance at the genus level.
authors.affiliation: College of Life Sciences/Key Laboratory of Microbiology Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, P. R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Lei
authors.affiliation: College of Life Sciences/Key Laboratory of Microbiology Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, P. R. China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: College of Life Sciences/Key Laboratory of Microbiology Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, P. R. China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Li
authors.affiliation: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P. R. China.
authors.forename: Chaoqiang
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P. R. China.
authors.forename: Chaolong
authors.initials: C
authors.lastname: Zu
authors.affiliation: Chizhou Tobacco Company of Anhui Province, Chizhou, 247000, P. R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Life Sciences/Key Laboratory of Microbiology Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, P. R. China. hcao@njau.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
mesh_terms: D000383:Agriculture / Q000379:methods*; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D002119:Calcium Carbonate; D002681:China; D018556:Crops, Agricultural; D005308:Fertilizers; D014644:Genetic Variation; D008274:Magnesium; D012275:Oryza; D018547:Plant Stems; D012399:Rotation; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana
chemical_list: D005308:Fertilizers; D012987:Soil; D002119:Calcium Carbonate; D008274:Magnesium; C028042:calcium magnesium carbonate
keywords: illumina sequencing; soil bacterial diversity and composition; straw mulching; tobacco-rice rotation