title: BRG1 regulation by miR-155 in human leukemia and lymphoma cell lines.
abstract: OBJECTIVE
  BRG1 is a key regulator of leukemia stem cells. Indeed, it has been observed that this type of cells is unable to divide, survive and develop new tumors when BRG1 is down-regulated.
  
  
  METHODS
  We assessed BRG1 and miR-155 expression in 23 leukemia cell lines, and two no pathological lymphocyte samples using qPCR. MiR-155 transfection and western blot were used to analyze the relationship between miR-155 and its validated target, BRG1, by measuring protein expression levels. The effect of miR-155 on cell proliferation and prednisolone sensitivity were studied with resazurin assay.
  
  
  RESULTS
  BRG1 expression levels could correlate negatively with miR-155 expression levels, at least in Burkitt's lymphoma and diffuse large B cell lymphoma (DLBCL) cell lines. To clarify the role of miR-155 in the regulation of BRG1 expression, we administrated miR-155 mimics in different leukemia/lymphoma cell lines. Our results suggest that miR-155 regulate negatively and significantly the BRG1 expression at least in the MOLT4 cell line.
  
  
  CONCLUSIONS
  Our study revealed a previously unknown miR-155 heterogeneity that could result in differences in the treatment with miRNAs in our attempt to inhibit BRG1. However, the expression levels of BRG1 and miR-155, before prednisolone treatment were not statistically significantly associated prednisolone sensitive leukemia cells.
authors.affiliation: Department of Biochemistry and Molecular Biology III and Immunology, University of Granada, Granada, Spain.|GENYO, Centre for Genomics and Oncological Research, Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Cuadros
authors.affiliation: Department of Biochemistry and Molecular Biology III and Immunology, University of Granada, Granada, Spain.|GENYO, Centre for Genomics and Oncological Research, Granada, Spain.
authors.forename: V
authors.identifier: http://orcid.org/0000-0003-1501-6681
authors.initials: V
authors.lastname: Sánchez-Martín
authors.affiliation: GENYO, Centre for Genomics and Oncological Research, Granada, Spain.|Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Herrera
authors.affiliation: GENYO, Centre for Genomics and Oncological Research, Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Baliñas
authors.affiliation: Department of Biochemistry and Molecular Biology III and Immunology, University of Granada, Granada, Spain.|GENYO, Centre for Genomics and Oncological Research, Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Martín-Padrón
authors.affiliation: Department of Biochemistry and Molecular Biology III and Immunology, University of Granada, Granada, Spain.|GENYO, Centre for Genomics and Oncological Research, Granada, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Boyero
authors.affiliation: GENYO, Centre for Genomics and Oncological Research, Granada, Spain.|Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Peinado
authors.affiliation: GENYO, Centre for Genomics and Oncological Research, Granada, Spain. pedromedina@ugr.es.|Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain. pedromedina@ugr.es.
authors.forename: P P
authors.initials: PP
authors.lastname: Medina
mesh_terms: D049109:Cell Proliferation; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008223:Lymphoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D014157:Transcription Factors; C084108:SMARCA4 protein, human; D004265:DNA Helicases
keywords: BRG1; Leukemia; Prednisolone; miR-155