title: Short-Chain Polysaccharide Analysis in Ethanol-Water Solutions.
abstract: This study demonstrates that short-chain polysaccharides, or oligosaccharides, could be sufficiently separated with hydrophilic interaction LC (HILIC) conditions and quantified by evaporative light-scattering detection (ELSD). The multianalyte calibration approach improved the efficiency of calibrating the nonlinear detector response. The method allowed easy quantification of short-chain carbohydrates. Using the HILIC method, the oligosaccharide solubility and its profile in water/alcohol solutions at room temperature were able to be quantified. The results showed that the polysaccharide solubility in ethanol-water solutions decreased as ethanol content increased. The results also showed oligosaccharides to have minimal solubility in pure ethanol. In a saturated maltodextrin ethanol (80%) solution, oligosaccharide components with a degree of polymerization >12 were practically insoluble and contributed less than 0.2% to the total solute dry weight. The HILIC-ELSD method allows for the identification and quantification of low-MW carbohydrates individually and served as an alternative method to current gel permeation chromatography procedures.
authors.affiliation: Amway Corp., Research and Development, 7575 Fulton St, Ada, MI 49301.
authors.forename: Xun
authors.initials: X
authors.lastname: Yan
mesh_terms: D000431:Ethanol; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D011134:Polysaccharides / Q000032:analysis*; D012542:Scattering, Radiation; D014867:Water
chemical_list: D011134:Polysaccharides; D014867:Water; D000431:Ethanol