title: Identification of potential therapeutic target genes, key miRNAs and mechanisms in oral lichen planus by bioinformatics analysis.
abstract: The study aimed to identify the potential target genes and key miRNAs as well as to explore the underlying mechanisms in the pathogenesis of oral lichen planus (OLP) by bioinformatics analysis. The microarray data of GSE38617 were downloaded from Gene Expression Omnibus (GEO) database. A total of 7 OLP and 7 normal samples were used to identify the differentially expressed genes (DEGs) and miRNAs. The DEGs were then performed functional enrichment analyses. Furthermore, DEG-miRNA network and miRNA-function network were constructed by Cytoscape software. Total 1758 DEGs (598 up- and 1160 down-regulated genes) and 40 miRNAs (17 up- and 23 down-regulated miRNAs) were selected. The up-regulated genes were related to nuclear factor-Kappa B (NF-κB) signaling pathway, while down-regulated genes were mainly enriched in the function of ribosome. Tumor necrosis factor (TNF), caspase recruitment domain family, member 11 (CARD11) and mitochondrial ribosomal protein (MRP) genes were identified in these functions. In addition, miR-302 was a hub node in DEG-miRNA network and regulated cyclin D1 (CCND1). MiR-548a-2 was the key miRNA in miRNA-function network by regulating multiple functions including ribosomal function. The NF-κB signaling pathway and ribosome function may be the pathogenic mechanisms of OLP. The genes such as TNF, CARD11, MRP genes and CCND1 may be potential therapeutic target genes in OLP. MiR-548a-2 and miR-302 may play important roles in OLP development.
authors.affiliation: Department of General Emergency, Dalian Hospital of Stomatology, Dalian, Liaoning, 116021, China.
authors.forename: Cuihua
authors.initials: C
authors.lastname: Gong
authors.affiliation: Department of Periodontal Mucosa, Dalian Hospital of Stomatology, Dalian, Liaoning, 116021, China.
authors.forename: Shangtong
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Orthopedic Trauma, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Cosmetic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, China. Electronic address: 1036174201@qq.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Reconstruction Surgery, Dalian Hospital of Stomatology, No. 935 Changjiang Rd, Shahekou District, Dalian, Liaoning, 116021, China. Electronic address: bingliu597@hotmail.com.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Chen
mesh_terms: D019295:Computational Biology / Q000379:methods*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D005819:Genetic Markers; D006801:Humans; D017676:Lichen Planus, Oral / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D015854:Up-Regulation
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha
keywords: Bioinformatics analysis; Differentially expressed genes; Differentially expressed miRNA; Oral lichen planus