title: Analysis of Naturally Occurring Phenolic Compounds in Aromatic Plants by RP-HPLC Coupled to Diode Array Detector (DAD) and GC-MS after Silylation.
abstract: The following aromatic plants of Greek origin,Origanum dictamnus(dictamus),Eucalyptus globulus(eucalyptus),Origanum vulgareL. (oregano),Mellisa officinalisL. (balm mint) andSideritis cretica(mountain tea), were examined for the content of phenolic substances. Reversed phase HPLC coupled to diode array detector (DAD) was used for the analysis of the plant extracts. The gas chromatography-mass spectrometry method (GC-MS) was also used for identification of phenolic compounds after silylation. The most abundant phenolic acids were: gallic acid (1.5-2.6 mg/100 g dry sample), ferulic acid (0.34-6.9 mg/100 g dry sample) and caffeic acid (1.0-13.8 mg/100 g dry sample). (+)-Catechin and (-)-epicatechin were the main flavonoids identified in oregano and mountain tea. Quercetin was detected only in eucalyptus and mountain tea.
authors.affiliation: Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771, Athens, Greece. harpro@chem.uoa.gr.
authors.forename: Charalampos
authors.initials: C
authors.lastname: Proestos
authors.affiliation: Laboratory of Food Chemistry and Analysis, Department of Food Science and Technology, Agricultural University of Athens, 11855, Athens, Greece. achem@aua.gr.
authors.forename: Michael
authors.initials: M
authors.lastname: Komaitis
keywords: GC-MS; RP-HPLC; aromatic plants; phenolic compounds; silylation