title: Genetic Diversity and Antibiotic Resistance of Enterococcus faecalis Isolates from Traditional Korean Fermented Soybean Foods.
abstract: Eighty-fiveEnterococcus faecalisisolates collected from animals (40 isolates), meju (a Korean fermented soybean product; 27 isolates), humans (10 isolates), and various environmental samples (8 isolates) were subjected to multilocus sequence typing (MLST) to identify genetic differences between samples of different origins. MLST analysis resulted in 44 sequence types (STs), and the eBURST algorithm clustered the STs into 21 clonal complexes (CCs) and 17 singletons. The predominant STs, ST695 (21.1%, 18/85) and ST694 (9.4%, 8/85), were singletons, and only contained isolates originating from meju. None of the STs in the current study belonged to CC2 or CC9, which comprise clinical isolates with high levels of antibiotic resistance. TheE. faecalisisolates showed the highest rates of resistance to tetracycline (32.9%), followed by erythromycin (9.4%) and vancomycin (2.4%). All isolates from meju were sensitive to these three antibiotics. Hence, MLST uncovered genetic diversity withinE. faecalis, and clustering of the STs using eBURST revealed a correlation between the genotypes and origins of the isolates.
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea.
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea.
authors.forename: Donghun
authors.initials: D
authors.lastname: Shin
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea.
authors.forename: Bitnara
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea.
authors.forename: Hyundong
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea.
authors.forename: Inhyung
authors.initials: I
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Dongduk Women's University, Seoul 02748, Republic of Korea.
authors.forename: Do-Won
authors.initials: DW
authors.lastname: Jeong
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004783:Environmental Microbiology; D004917:Erythromycin / Q000494:pharmacology; D005285:Fermentation; D005516:Food Microbiology; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D058885:Multilocus Sequence Typing / Q000379:methods / Q000662:veterinary; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D013025:Glycine max / Q000382:microbiology*; D013552:Swine; D013752:Tetracycline / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004917:Erythromycin; D014640:Vancomycin; D013752:Tetracycline
keywords: Enterococcus faecalis; antibiotic resistance; diversity; meju; multilocus sequence typing