title: The Biomineralization of a Bioactive Glass-Incorporated Light-Curable Pulp Capping Material Using Human Dental Pulp Stem Cells.
abstract: The aim of this study was to investigate the biomineralization of a newly introduced bioactive glass-incorporated light-curable pulp capping material using human dental pulp stem cells (hDPSCs). The product (Bioactive® [BA]) was compared with a conventional calcium hydroxide-incorporated (Dycal [DC]) and a light-curable (Theracal® [TC]) counterpart. Eluates from set specimens were used for investigating the cytotoxicity and biomineralization ability, determined by alkaline phosphatase (ALP) activity and alizarin red staining (ARS). Cations and hydroxide ions in the extracts were measured. An hDPSC viability of less than 70% was observed with 50% diluted extract in all groups and with 25% diluted extract in the DC. Culturing with 12.5% diluted BA extract statistically lowered ALP activity and biomineralization compared to DC (p< 0.05), but TC did not (p> 0.05). Ca (~110 ppm) and hydroxide ions (pH 11) were only detected in DC and TC. Ionic supplement-added BA, which contained similar ion concentrations as TC, showed similar ARS mineralization compared to TC. In conclusion, the BA was similar to, yet more cytotoxic to hDPSCs than, its DC and TC. The BA was considered to stimulate biomineralization similar to DC and TC only when it released a similar amount of Ca and hydroxide ions.
authors.affiliation: Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, Republic of Korea.
authors.forename: Soo-Kyung
authors.initials: SK
authors.lastname: Jun
authors.affiliation: Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, Republic of Korea.
authors.forename: Jung-Hwan
authors.identifier: 0000-0001-8678-5459
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, Republic of Korea.
authors.forename: Hae-Hyoung
authors.initials: HH
authors.lastname: Lee
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D055117:Curing Lights, Dental; D003764:Dental Materials / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology*; D003785:Dental Pulp Capping; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007477:Ions; D008903:Minerals / Q000737:chemistry*; D013054:Spectrophotometry, Atomic; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D003764:Dental Materials; D007477:Ions; D008903:Minerals