title: [Effects of Danshensu on bone formation in ovariectomized rats].
abstract: Objective:To investigate the effects of Danshensu on bone formation in ovariectomized rats.Methods:Thirty female SD rats were randomly divided into three groups with 10 rats in each:blank control group, model control group and Danshensu group. The osteoporosis model was induced by bilateral ovariectomy and rats in Danshensu group were fed with Danshensu 12.5 mg·kg-1·d-1by gavage after ostroporosis model induced. All animals were sacrificed after 90 days. The bone mineral density (BMD) of the whole body, femur and lumbar vertebra was measured by dual energy X-ray absorptiometry. The biomechanical properties of femur were measured by AG-IS mechanical universal testing machine. Serum osteocalcin and bone alkaline phosphates (BALP) levels were measured by ELISA. The number of osteoblasts of proximal femoral metaphysis was counted with light microscopy after HE staining.Results:Compared with blank control group, BMD, biomechanical properties of femur, serum osteocalcin and BALP levels and the number of osteoblasts were decreased in model control group (P<0.05 orP<0.01). While compared with model control group, BMDs of the whole body, femur and lumbar vertebra, the elastic modulus, maximum load, yield strength, breaking point load of femur, the serum levels of osteocalcin and BALP, and the number of osteoblasts were significantly improved in Danshensu group (P<0.05 orP<0.01).Conclusion:Danshensu can improve bone quality by increasing bone density, improving biomechanical properties, promoting the expression of osteogenesis-related factors, and increasing the number of osteoblasts.
authors.affiliation: Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qu
authors.affiliation: Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Yang
authors.affiliation: Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lan
authors.affiliation: Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China. wsyonggansu@126.com.
authors.forename: Shiyong
authors.initials: S
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood / Q000187:drug effects; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects*; D015519:Bone Density / Q000187:drug effects*; D002452:Cell Count; D005260:Female; D005269:Femur / Q000033:anatomy & histology* / Q000166:cytology* / Q000187:drug effects*; D007773:Lactates / Q000494:pharmacology*; D008159:Lumbar Vertebrae / Q000033:anatomy & histology / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects; D015675:Osteocalcin / Q000097:blood / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D010024:Osteoporosis / Q000188:drug therapy; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007773:Lactates; D015675:Osteocalcin; C035055:3,4-dihydroxyphenyllactic acid; D000469:Alkaline Phosphatase