title: Conditions for Collection of Placental Tissue Samples for Culturing of Multipotent Mesenchymal Stromal Cells.
abstract: Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients' histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory.
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Nizyaeva
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Nagovitsyna
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Kulikova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: U N
authors.initials: UN
authors.lastname: Tumanova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: R A
authors.initials: RA
authors.lastname: Poltavtseva
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Fedorova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Yushina
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Pavlovich
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia. ashegolev@oparina4.ru.
authors.forename: A I
authors.initials: AI
authors.lastname: Shchyogolev
mesh_terms: D000328:Adult; D000650:Amnion / Q000166:cytology* / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D016203:CDC2 Protein Kinase; D002454:Cell Differentiation; D002824:Chorionic Villi / Q000378:metabolism / Q000648:ultrastructure; D056744:Cyclin B1 / Q000235:genetics / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D007231:Infant, Newborn; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D061251:Primary Cell Culture; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D013048:Specimen Handling / Q000592:standards*; D014470:Umbilical Cord / Q000166:cytology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C000595707:BCL2 protein, human; D015415:Biomarkers; C534699:CCNB1 protein, human; D056744:Cyclin B1; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases
keywords: amnion; cell culturing; multipotent mesenchymal stromal cells; placenta; umbilical cord