title: Comparative transcriptomic analysis reveals the roles of overlapping heat-/drought-responsive genes in poplars exposed to high temperature and drought.
abstract: High temperature (HT) and drought are both critical factors that constrain tree growth and survival under global climate change, but it is surprising that the transcriptomic reprogramming and physiological relays involved in the response to HT and/or drought remain unknown in woody plants. Thus, Populus simonii saplings were exposed to either ambient temperature or HT combined with sufficient watering or drought. RNA-sequencing analysis showed that a large number of genes were differentially expressed in poplar roots and leaves in response to HT and/or desiccation, but only a small number of these genes were identified as overlapping heat-/drought-responsive genes that are mainly involved in RNA regulation, transport, hormone metabolism, and stress. Furthermore, the overlapping heat-/drought-responsive genes were co-expressed and formed hierarchical genetic regulatory networks under each condition compared. HT-/drought-induced transcriptomic reprogramming is linked to physiological relays in poplar roots and leaves. For instance, HT- and/or drought-induced abscisic acid accumulation and decreases in auxin and other phytohormones corresponded well with the differential expression of a few genes involved in hormone metabolism. These results suggest that overlapping heat-/drought-responsive genes will play key roles in the transcriptional and physiological reconfiguration of poplars to HT and/or drought under future climatic scenarios.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.|College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Cao
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Büsgen-Institute, Department of Forest Botany and Tree Physiology, Georg-August University, Büsgenweg 2, 37077 Göttingen, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Polle
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Luo
mesh_terms: D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D006358:Hot Temperature; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D032107:Populus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological