title: Tamarix gallica phenolics protect IEC-6 cells against H2O2induced stress by restricting oxidative injuries and MAPKs signaling pathways.
abstract: Polyphenolic compounds gained interest in the pharmaceutical research area due to their beneficial properties. Herein, antioxidant and cytoprotective capacities of T. gallica extract on H2O2-challenged rat small intestine epithelial cells were investigated. To set stress conditions, IEC-6 cultures were challenged with numerous H2O2doses and durations. Then, 40μM H2O2during 4h were selected to assess the cytoprotective effect of different T. gallica extract concentrations. Oxidative parameters, measured through CAT and SOD activities as well as MDA quantification were assessed. In addition, the expression of possibly involved MAPKs was also valued. Main results reported that T. gallica was rich in polyphenols and exhibited an important antioxidant activity (DPPH Assay, IC50=6μgmL-1; ABTS+test, IC50=50μgmL-1; Fe-reducing power, EC50=100μgmL-1). The exposure of IEC-6 cultures to 40μM H2O2during 4h caused oxidative stress manifested by (i) over 70% cell mortality, (ii) over-activity of CAT (246%), (iii) excess in MDA content (18.4nmolmg-1) and (iiii) a trigger of JNK phosphorylation. Pretreatment with T. gallica extract, especially when used at 0.25μgmL-1, restored cell viability to 122%, and normal cell morphology in H2O2-chalenged cells. In addition, this extract normalized CAT activity and MDA content (100% and 14.7nmolmg-1, respectively) to their basal levels as compared to control cells. Furthermore, stopping cell death seems to be due to dephosphorylated JNK MAPK exerted by T. gallica bioactive compounds. In all, T. gallica components provided a cross-talk between regulatory pathways leading to an efficient cytoprotection against harmful oxidative stimulus.
authors.affiliation: Université de Tunis El Manar, Faculté des Sciences de Tunis, 2092, Tunis, Tunisie; Centre de Biotechnologie de Borj-Cédria, LR15CBBC06 Laboratoire des Plantes Aromatiques et Médicinales, BP 901, 2050 Hammam-lif, Tunisie; Université de Bretagne Occidentale, Faculté de Médecine et des Sciences de la Santé, EA 1274 Laboratoire de Physiologie, 29200, Brest, France. Electronic address: jbettaib@gmail.com.
authors.forename: Jamila
authors.initials: J
authors.lastname: Bettaib
authors.affiliation: Université de Bretagne Occidentale, Faculté de Médecine et des Sciences de la Santé, EA 1274 Laboratoire de Physiologie, 29200, Brest, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Talarmin
authors.affiliation: Université de Bretagne Occidentale, Faculté de Médecine et des Sciences de la Santé, EA 1274 Laboratoire de Physiologie, 29200, Brest, France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Droguet
authors.affiliation: Université de Bretagne Occidentale, UFR Sciences et Techniques, EA 2219 Géoarchitecture, CS 93837, 29238 Brest Cedex 3, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Magné
authors.affiliation: Centre de Biotechnologie de Borj-Cédria, LR15CBBC06 Laboratoire des Plantes Aromatiques et Médicinales, BP 901, 2050 Hammam-lif, Tunisie.
authors.forename: Mondher
authors.initials: M
authors.lastname: Boulaaba
authors.affiliation: Université de Bretagne Occidentale, Faculté de Médecine et des Sciences de la Santé, EA 1274 Laboratoire de Physiologie, 29200, Brest, France.
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Giroux-Metges
authors.affiliation: Centre de Biotechnologie de Borj-Cédria, LR15CBBC06 Laboratoire des Plantes Aromatiques et Médicinales, BP 901, 2050 Hammam-lif, Tunisie.
authors.forename: Riadh
authors.initials: R
authors.lastname: Ksouri
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000166:cytology; D020929:Mitogen-Activated Protein Kinase Kinases; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects*; D032363:Tamaricaceae / Q000737:chemistry*
chemical_list: D010636:Phenols; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: Antioxidants; Cytoprotection; H(2)O(2)-induced oxidative stress; IEC-6 cells; Tamarix gallica