title: [Morphometric characterization of the subbasal nerve plexus : Detection and analysis of networks of nerve fibers].
abstract: Confocal laser scanning microscopy is a versatile tool in medical research and enables noninvasive in vivo imaging of the corneal subbasal nerve plexus. The aim of this work is to provide a structured overview about the detection and quantification of nerve fibers of the subbasal nerve plexus from images acquired by confocal laser scanning microscopy. Relevant steps are explained and potential factors influencing the quality of the results are pointed out. Information obtained from the quantification of subbasal nerve fiber structure can be potentially used as clinical parameters in the context of diagnostics and therapy control of diabetic neuropathy.
authors.affiliation: Institut für Anatomie, Medizinische Fakultät, Universität Leipzig, Liebigstr. 13, 04103, Leipzig, Deutschland. kwinter@rz.uni-leipzig.de.
authors.forename: K
authors.initials: K
authors.lastname: Winter
authors.affiliation: Sächsischer Inkubator für Klinische Translation (SIKT) Leipzig, Universität Leipzig, Philipp-Rosenthal-Str. 55, Leipzig, 04103, Deutschland.
authors.forename: P
authors.initials: P
authors.lastname: Scheibe
authors.affiliation: Universitätsaugenklinik, Universitätsmedizin Rostock, Doberaner Str. 140, Rostock, 18057, Deutschland.
authors.forename: R F
authors.initials: RF
authors.lastname: Guthoff
authors.affiliation: Institut für Angewandte Informatik, Karlsruher Institut für Technologie, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen, 76344, Deutschland.
authors.forename: S
authors.initials: S
authors.lastname: Allgeier
authors.affiliation: Universitätsaugenklinik, Universitätsmedizin Rostock, Doberaner Str. 140, Rostock, 18057, Deutschland.
authors.forename: O
authors.initials: O
authors.lastname: Stachs
mesh_terms: D003315:Cornea / Q000294:innervation*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D000069416:Intravital Microscopy / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D009412:Nerve Fibers / Q000473:pathology*; D009415:Nerve Net / Q000473:pathology*; D012680:Sensitivity and Specificity
keywords: Confocal microscopy; Cornea; Laser scanning microscopy; Morphology; Quantification