title: Zinc oxide nanoparticles hepatotoxicity: Histological and histochemical study.
abstract: Zinc oxide nanoparticles (ZnO NPs) are widely used in industry and cosmetic products with promising investment in medical diagnosis and treatment. However, these particles may reveal a high potential risk for human health with no information about hepatotoxicity that might be associated with their exposure. The present work was carried out to investigate the histological and histochemical alterations induced in the hepatic tissues by naked 35nm ZnO NPs. Male Wistar albino rats were exposed to ZnO NPs at a daily dose of 2mg/kg for 21days. Liver biopsies from all rats under study were subjected to histopathological examinations. In comparison with the control rats, the following histological and histochemical alterations were demonstrated in the hepatic tissues of rats exposed to ZnO NPs: sinusoidal dilatation, Kupffer cells hyperplasia, lobular and portal triads inflammatory cells infiltration, necrosis, hydropic degeneration, hepatocytes apoptosis, anisokaryosis, karyolysis, nuclear membrane irregularity, glycogen content depletion and hemosidrosis. The findings of the present work might indicate that ZnO NPs have potential oxidative stress in the hepatic tissues that may affect the function of the liver. More work is needed to elucidate the toxicity and pathogenesis of zinc oxide nanoparticles on the vital organs.
authors.affiliation: Zoology Department, College of Science, King Saud University, Saudi Arabia. Electronic address: mmansour@ksu.edu.sa.
authors.forename: Mansour I
authors.initials: MI
authors.lastname: Almansour
authors.affiliation: Biology Department, College of Science-Onizah, Qassim University, Saudi Arabia. Electronic address: mosaidaf@yahoo.com.
authors.forename: Mosaid A
authors.initials: MA
authors.lastname: Alferah
authors.affiliation: Department of Biological Sciences, College of Science, The University of Jordan, Jordan. Electronic address: zaid-shr@ju.edu.jo.
authors.forename: Ziad A
authors.initials: ZA
authors.lastname: Shraideh
authors.affiliation: Department of Biological Sciences, College of Science, Jerash University, Jordan. Electronic address: bashirjarrar@yahoo.com.
authors.forename: Bashir M
authors.initials: BM
authors.lastname: Jarrar
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D006003:Glycogen / Q000378:metabolism*; D006486:Hemosiderosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D009336:Necrosis; D010316:Particle Size; D017208:Rats, Wistar; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry / Q000633:toxicity*
chemical_list: D006003:Glycogen; D015034:Zinc Oxide
keywords: Glycogen depletion; Hemosidrosis; Hepatotoxicity; Hydropic degeneration; Nanotoxicity; ZnO NPs