title: Research survey on iridoid and phenylethanoid glycosides among seven populations of Euphrasia rostkoviana Hayne from the Alps.
abstract: The traditional use of extracts of Euphrasia rostkoviana to relieve ocular inflammation or infections is well documented and supported by clinical studies. Various classes of chemical compounds such as iridoids, phenylethanoids, flavonoids and hydroxycinnamic acids have been reported. The present work aims to assess the chemical diversity among seven populations of Euphrasia rostkoviana found in northern Italy. A meticulous separation of components led to the isolation and structural characterization of two previously unrecorded phenylethanoids methoxycrassifolioside and deoxycrassifolioside and one previously undescribed terpene glucoside (1E,6E)-8-hydroxy-3,7-dimethyl-octa-1,6-dienyl 1-O-β-D-glucopyranoside. We have also identified known phenylethanoids and iridoids that are reported in this genus for the first time. Finally, a targeted quantitative analysis for the standardization of herbal preparations revealed that iridoids occur in all populations whereas the presence and the levels of rutin and phenylethanoids are highly variable.
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, 84084 Fisciano (SA), Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Mari
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Physics, Università degli Studi di Trento, 38123 Trento, Italy.
authors.forename: Alexandru
authors.initials: A
authors.lastname: Ciocarlan
authors.affiliation: Council for Agricultural Research and Economics, Forest Monitoring and Management Research Unit, 38123 Trento, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Aiello
authors.affiliation: Council for Agricultural Research and Economics, Forest Monitoring and Management Research Unit, 38123 Trento, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Scartezzini
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, 84084 Fisciano (SA), Italy.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Physics, Università degli Studi di Trento, 38123 Trento, Italy. Electronic address: michele.dambrosio@unitn.it.
authors.forename: Michele
authors.initials: M
authors.lastname: D'Ambrosio
mesh_terms: D032201:Euphrasia / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D039823:Iridoids / Q000737:chemistry* / Q000302:isolation & purification; D007558:Italy; D015394:Molecular Structure; D064209:Phytochemicals / Q000737:chemistry / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; D039823:Iridoids; D064209:Phytochemicals; D010936:Plant Extracts
keywords: Aucubin (PubChem CID: 91458); Catalpol (PubChem CID: 91520); Citronellenyl glucoside; Crassifolioside; Crassifolioside (PubChem CID: 102193658); Ehrenoside; Ehrenoside (PubChem CID: 10259536); Euphrasia rostkoviana; Geniposidic acid (PubChem CID: 443354); Iridoids; Mussaenosidic acid (PubChem CID: 21633105); Orobanchaceae; Phenylethanoid glycosides; Rutin (PubChem CID: 5280805); Salidroside (PubChem CID: 159278); Verbascoside; Verbascoside (PubChem CID: 5281800)