title: Differential Gene Expression Network in Terpenoid Synthesis of Antrodia cinnamomea in Mycelia and Fruiting Bodies.
abstract: Antodia cinnamomea, a precious brown-rot fungus endemic to Taiwan, has pharmaceutical applications due to its diverse array of metabolites. The terpenoids found in A. cinnamomea contribute to its most important bioactivities. We identified several terpenoid compounds in A. cinnamomea and revealed that their content in mycelium and fruiting body were significantly different. Using next-generation sequencing and an in-house transcriptome database, we identified several terpene synthase (TPS) candidates. After sequence analysis and functional characterization, 10 out of 12 candidates were found to have single or multiple terpene synthesis functions. Most of the terpenoid compounds were found to confer important bioactivities. RT-PCR results showed a positive correlation between terpene synthase expression pattern and terpenoid content. In addition, we identified several modification enzyme candidates that may be involved in the postmodification of terpenoid compounds with a genomic DNA scaffold, and a putative genetic network.
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University , Taipei, Taiwan.
authors.forename: Yan-Liang
authors.initials: YL
authors.lastname: Lin
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University , Taipei, Taiwan.
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Ma
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University , Taipei, Taiwan.
authors.forename: Yi-Ru
authors.initials: YR
authors.lastname: Lee
authors.affiliation: I-Shou University , Kaohsiung, Taiwan.
authors.forename: Jei-Fu
authors.initials: JF
authors.lastname: Shaw
authors.affiliation: Department of Forestry, National Chung-Hsing University , Taichun, Taiwan.|Agricultural, Biotechnology Research Center, Academia Sinica , Taipei, Taiwan.
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Wang
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University , Taipei, Taiwan.
authors.forename: Fang-Hua
authors.identifier: http://orcid.org/0000-0003-2299-5013
authors.initials: FH
authors.lastname: Chu
mesh_terms: D055491:Antrodia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D053263:Gene Regulatory Networks; D025282:Mycelium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D005656:Fungal Proteins; D013729:Terpenes
keywords: Antrodia cinnamomea; genetic network; next-generation sequencing; terpenoid synthase; transcriptome