title: Proteomic Analysis of Responsive Proteins Induced in Japanese Birch Plantlet Treated with Salicylic Acid.
abstract: The present study was performed to unravel the mechanisms of systemic acquired resistance (SAR) establishment and resistance signaling pathways against the canker-rot fungus (Inonotus obliquusstrain IO-U1) infection in Japanese birch plantlet No.8. Modulation of protein-profile induced by salicylic acid (SA)-administration was analyzed, and SA-responsive proteins were identified. In total, 5 specifically expressed, 3 significantly increased, and 3 significantly decreased protein spots were identified using liquid chromatography/tandem mass spectrometry (LC/MS/MS) and the sequence tag method. These proteins were malate dehydrogenase, succinate dehydrogenase, phosphoglycerate kinase, diaminopimalate decarboxylase, arginase, chorismate mutase, cyclophilin, aminopeptidase, and unknown function proteins. These proteins are considered to be involved in SAR-establishment mechanisms in the Japanese birch plantlet No 8.
authors.affiliation: Department of Forest Science, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan. skaku@ba2.so-net.ne.jp.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Department of Forest Science, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan. ytakashima@ffpri.affrc.go.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Takashima
authors.affiliation: Department of Forest Science, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan. ishiguri@cc.utsunomiya-u.ac.jp.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Ishiguri
authors.affiliation: Department of Forest Science, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yoshizawa
authors.affiliation: Department of Forest Science, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan. yokotas@cc.utsunomiya-u.ac.jp.
authors.forename: Shinso
authors.initials: S
authors.lastname: Yokota
keywords: Inonotus obliquus; Japanese birch; salicylic acid