title: Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model.
abstract: Our previous studies revealed that many proteins in addition to the known allergens of D. farinae have not been fully characterized. We observed that Pplase did not respond to serum collected from patients sensitized to D. farinae. In a mouse model, Pplase significantly enhanced airway hyperresponsiveness (AHR) and Th2 responses induced by ovalbumin (OVA) compared with mice treated with OVA alone. Moreover, exposure to Pplase significantly increased the expression of IRF4, CD80, CD83, MHCII and TNF-α in DC2.4 cells, which was abolished in the presence of a TLR4 inhibitor. In vitro T cell polarization experiments revealed that Pplase alone could not induce T cell polarization but enhanced T cell polarization together with OVA. In addition, transfer of Pplase-primed bone marrow-derived DCs (BMDCs) to naïve mice enhanced AHR and Th2 immune responses in mice sensitized to OVA. In conclusion, Pplase is not an allergen of D. farinae but can activate DC cells to facilitate OVA-induced allergic responses.
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Shenzhen ENT Institute, Longgang ENT Hospital, Shenzhen, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Mo
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Luohu district people's hospital, Shenzhen, China.
authors.forename: Shu
authors.initials: S
authors.lastname: An
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Jinge
authors.initials: J
authors.lastname: Ba
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Weifang
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510006, China.
authors.forename: Pixin
authors.initials: P
authors.lastname: Ran
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Shenzhen ENT Institute, Longgang ENT Hospital, Shenzhen, China.
authors.forename: Pingchang
authors.initials: P
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Shenzhen ENT Institute, Longgang ENT Hospital, Shenzhen, China.|Luohu district people's hospital, Shenzhen, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000328:Adult; D000485:Allergens / Q000008:administration & dosage; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D060829:Arthropod Proteins / Q000494:pharmacology*; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000473:pathology / Q000637:transplantation; D040001:Dermatophagoides farinae / Q000737:chemistry / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology; D050835:Interferon Regulatory Factors / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008875:Middle Aged; D010047:Ovalbumin / Q000008:administration & dosage; D019696:Peptidylprolyl Isomerase / Q000494:pharmacology*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D000097802:CD83 Antigen; D000099218:Interferon Regulatory Factor-4
chemical_list: D000485:Allergens; D015703:Antigens, CD; D060829:Arthropod Proteins; D018122:B7-1 Antigen; D051792:Basic Helix-Loop-Helix Proteins; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins; D050835:Interferon Regulatory Factors; D008562:Membrane Glycoproteins; D010047:Ovalbumin; D019696:Peptidylprolyl Isomerase; D018336:Receptors, Aryl Hydrocarbon; D013449:Sulfonamides; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D000097802:CD83 Antigen; D000099218:Interferon Regulatory Factor-4; C484722:Ahr protein, mouse; C493487:Tlr4 protein, mouse; C507035:ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-ene-1-carboxylate