title: Catch and Release: rare cell analysis from a functionalised medical wire.
abstract: Enumeration and especially molecular characterization of circulating tumour cells (CTCs) holds great promise for cancer management. We tested a modified type of an in vivo enrichment device (Catch&Release) for its ability to bind and detach cancer cells for the purpose of single-cell molecular downstream analysis in vitro. The evaluation showed that single-cell analysis using array comparative genome hybridization (array-CGH) and next generation sequencing (NGS) is feasible. We found array-CGH to be less noisy when whole genome amplification (WGA) was performed with Ampli1 as compared to GenomePlex (DLRS values 0.65 vs. 1.39). Moreover, Ampli1-processed cells allowed detection of smaller aberrations (median 14.0 vs. 49.9 Mb). Single-cell NGS data obtained from Ampli1-processed samples showed the expected non-synonymous mutations (deletion/SNP) according to bulk DNA. We conclude that clinical application of this refined in vivo enrichment device allows CTC enumeration and characterization, thus, representing a promising tool for personalized medicine.
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
authors.forename: Shukun
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
authors.forename: Amin
authors.initials: A
authors.lastname: El-Heliebi
authors.affiliation: Department of Therapeutic Radiology and Oncology, Medical University of Graz, Graz, Austria.
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Tauber
authors.affiliation: Department of Therapeutic Radiology and Oncology, Medical University of Graz, Graz, Austria.
authors.forename: Tanja
authors.initials: T
authors.lastname: Langsenlehner
authors.affiliation: Department of Therapeutic Radiology and Oncology, Medical University of Graz, Graz, Austria.
authors.forename: Michaela
authors.initials: M
authors.lastname: Pötscher
authors.affiliation: Institute of Pathology, Medical University of Graz, Graz, Austria.
authors.forename: Karl
authors.initials: K
authors.lastname: Kashofer
authors.affiliation: Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, Regensburg, Germany.
authors.forename: Zbigniew T
authors.initials: ZT
authors.lastname: Czyż
authors.affiliation: Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, Regensburg, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Polzer
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Riethdorf
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Andra
authors.initials: A
authors.lastname: Kuske
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
authors.forename: Gerd
authors.initials: G
authors.lastname: Leitinger
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Pantel
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.|Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kroneis
authors.affiliation: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Sedlmayr
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D002452:Cell Count; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D055028:Comparative Genomic Hybridization; D000071858:Epithelial Cell Adhesion Molecule / Q000235:genetics / Q000378:metabolism; D004867:Equipment Design; D015870:Gene Expression; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D057285:Precision Medicine; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D000906:Antibodies; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule