title: C-terminal dimerization of apo-cyclic AMP receptor protein validated in solution.
abstract: Although cyclic AMP receptor protein (CRP) has long served as a typical example of effector-mediated protein allostery, mechanistic details into its regulation have been controversial due to discrepancy between the known crystal structure and NMR structure of apo-CRP. Here, we report that the recombinant protein corresponding to its C-terminal DNA-binding domain (CDD) forms a dimer. This result, together with structural information obtained in the present NMR study, is consistent with the previous crystal structure and validates its relevance also in solution. Therefore, our findings suggest that dissociation of the CDD may be critically involved in cAMP-induced allosteric activation of CRP.
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Korea.
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Sim
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Korea.
authors.forename: Jae Wan
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Korea.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Protein Structure Group, Korea Basic Science Institute, Ochang, Chungbuk, Korea.
authors.forename: Kyoung-Seok
authors.initials: KS
authors.lastname: Ryu
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Korea.
authors.forename: Myeongkyu
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Korea.
authors.forename: Hee-Wan
authors.initials: HW
authors.lastname: Yu
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Korea.
authors.forename: Ku-Sung
authors.initials: KS
authors.lastname: Jo
authors.affiliation: Protein Structure Group, Korea Basic Science Institute, Ochang, Chungbuk, Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, Gyeonggi, Korea.|Research Institute of Pharmaceutical Science and Technology, College of Pharmacy, Ajou University, Suwon, Gyeonggi, Korea.
authors.forename: Min-Duk
authors.initials: MD
authors.lastname: Seo
authors.affiliation: College of Pharmacy, Korea University, Sejong, Korea.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Jeon
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.
authors.forename: Bong-Jin
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: Department of Bio-Engineering, Life Science RD Center, Sinil Pharmaceutical Co., Seongnam, Gyeonggi, Korea.
authors.forename: Young Pil
authors.initials: YP
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Research Institute (RIBHS) and College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Korea.
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Won
mesh_terms: D000595:Amino Acid Sequence; D001059:Apoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002942:Circular Dichroism; D000242:Cyclic AMP / Q000737:chemistry / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011485:Protein Binding; D000072417:Protein Domains; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012996:Solutions / Q000737:chemistry*
chemical_list: D001059:Apoproteins; D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D012996:Solutions; D000242:Cyclic AMP
keywords: NMR spectroscopy; cyclic AMP receptor protein; protein allostery