title: Antibacterial evaluation of sliver nanoparticles synthesized by polysaccharides from Astragalus membranaceus roots.
abstract: This work introduced an eco-friendly approach for the synthesis of sliver nanoparticles (AgNPs) with a water-soluble fraction of polysaccharides extracted from the roots of Astragalus membranaceus (AMWP). AMWP mediated AgNPs (AMWP-AgNPs) were successfully synthesized using 1.0mM AgNO3and 1.25mg/mL AMWP, reacting in aqueous environment at 25°C for 6h. The synthesized AMWP-AgNPs exhibited a broad surface plasmon resonance peak (SPR) at 445nm in Ultraviolet-visible (UV-vis) spectrum, which was characteristic of a poly-dispersed AMWP-AgNPs. Transmission electron microscopy (TEM) and Dynamic light scattering (DLS) analysis revealed that the AMWP-AgNPs were almost spherical shaped with an average diameter of 65.08nm. The antibacterial activities of AMWP-AgNPs were assessed on four strains of clinical isolated multidrug resistant (MDR) bacteria and four relative reference strains using modified agar disk diffusion and tetrazolium methods. All strains, especially the resistant strains, were significantly inhibited by AMWP-AgNPs at comparatively low concentration (minimum inhibition concentrations (MICs) ranging from 0.032 to 0.063mg/mL). Therefore, we propose that the AMWP-AgNPs will have a great potential in anti-MDR bacterial applications.
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China; Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China; Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China.
authors.forename: Congyan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China; Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China.
authors.forename: Ding
authors.initials: D
authors.lastname: Qu
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China; Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China. Electronic address: ychen202@hotmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China; Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Huang
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China; Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D027885:Astragalus propinquus / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D011134:Polysaccharides; D012834:Silver
keywords: AgNPs; Astragalus membranaceus root; Multidrug resistant bacteria; Polysaccharides