title: MicroRNA biomarkers of Parkinson's disease in serum exosome-like microvesicles.
abstract: Parkinson's disease (PD) is a progressive age-related debilitating motor disorder and the second most common neurodegenerative disease after Alzheimer's disease. In this study, we aimed to investigate the expression of 24 candidate miRNAs in PD and to assess their diagnostic value in patients with PD. We collected serum samples from 109 patients with PD and 40 age- and sex-matched healthy volunteers (control group). RNAs encapsulated in exosome-like microvesicles in serum were extracted and reverse transcribed. Serum miRNAs were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and the ability of the miRNAs to accurately discriminate PD was analyzed by receiver operating characteristic curves. Based on our analysis, we further validated the downregulation of miR-19b and the upregulation of miR-195 and miR-24 in patients with PD. When compared with the control group, the area under the curve (AUC) values for miR-19b, miR-24, and miR-195 were 0.753, 0.908, and 0.697, respectively. Therefore, analysis of the expression levels of miR-19b, miR-24, and miR-195 in serum may be useful for the diagnosis of PD.
authors.affiliation: Department of Neurology, Huai'an Hospital Affiliated to Xuzhou Medical University, 62 Huaihan South Road, Huai'an, Jiangsu, 223002, PR China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Cao
authors.affiliation: Department of Neurology, Huai'an Hospital Affiliated to Xuzhou Medical University, 62 Huaihan South Road, Huai'an, Jiangsu, 223002, PR China.
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Lu
authors.affiliation: Department of Neurology, Huai'an Hospital Affiliated to Xuzhou Medical University, 62 Huaihan South Road, Huai'an, Jiangsu, 223002, PR China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, PR China.
authors.forename: Ming-Chao
authors.initials: MC
authors.lastname: Li
authors.affiliation: Department of Neurology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Neurology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, PR China.
authors.forename: Xu-Sheng
authors.initials: XS
authors.lastname: An
authors.affiliation: Department of Neurology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, PR China. Electronic address: liujunxue008@126.com.
authors.forename: Liu-Jun
authors.initials: LJ
authors.lastname: Xue
mesh_terms: D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood*; D055252:Cell-Derived Microparticles; D055354:Exosomes; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D010300:Parkinson Disease / Q000097:blood*; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; C549214:MIRN19A microRNA, human; C552722:MIRN195 microRNA, human; C543845:MIRN24 microRNA, human; D035683:MicroRNAs
keywords: Parkinson’s disease; Serum-based biomarker; microRNA-195; microRNA-19b; microRNA-24