title: Heterogeneity of Retinal Pigment Epithelial Cells from Adult Human Eye in Different Culturing Systems.
abstract: We studied the behavior of retinal pigment epithelial cells from adult human eye derived from different donors in different culturing systems: on plastic, in collagen gel, and on decellularized neural retina substrate. The cells diverge into two subpopulations similar by their morphology and behavior: one subpopulation migrated to the surface of the dense substrate and the other formed spheroid structures consisting of aggregated cells. This fact confirms the data on genetically-predetermined phenotypic heterogeneity of retinal pigment epithelium cells that should be taken into account when using these cells in tissue engineering.
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. avkuzn@list.ru.
authors.forename: A V
authors.initials: AV
authors.lastname: Kuznetsova
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Aleksandrova
mesh_terms: D000328:Adult; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D005109:Extracellular Matrix / Q000737:chemistry / Q000187:drug effects; D005782:Gels; D006801:Humans; D008875:Middle Aged; D010641:Phenotype; D010969:Plastics / Q000737:chemistry / Q000494:pharmacology; D061251:Primary Cell Culture; D051381:Rats; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000187:drug effects; D018874:Spheroids, Cellular / Q000166:cytology* / Q000187:drug effects; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D005782:Gels; D010969:Plastics; D003094:Collagen
keywords: 3D matrix; cell culture; collagen gel; devitalization of the retina; retinal pigment epithelium from adult human eye