title: Air-Liquid Interface Cell Exposures to Nanoparticle Aerosols.
abstract: The field of nanomedicine is steadily growing and several nanomedicines are currently approved for clinical use with even more in the pipeline. Yet, while the use of nanotechnology to improve targeted drug delivery to the lungs has received some attention, the use of nanoparticles for inhalation drug delivery has not yet resulted in successful translation to market as compared to intravenous drug delivery. The reasons behind the lack of inhaled nanomedicines approved for clinical use or under preclinical development are unclear, but challenges related to safety are likely to contribute. Although inhalation toxicology studies often begin using animal models, there has been an increase in the development and use of in vitro air-liquid interface (ALI) exposure systems for toxicity testing of engineered nanoparticle aerosols, which will be useful for rapid testing of candidate substances and formulations. This chapter describes an ALI cell exposure assay for measuring toxicological effects, specifically cell viability and oxidative stress, resulting from exposure to aerosols containing nanoparticles.
authors.affiliation: Institute for Work and Health (IST), University of Lausanne, Lausanne, Switzerland. nalewinski@vcu.edu.|Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA, USA. nalewinski@vcu.edu.
authors.forename: Nastassja A
authors.initials: NA
authors.lastname: Lewinski
authors.affiliation: Institute for Work and Health (IST), University of Lausanne, Lausanne, Switzerland.|Department of Medicine, Weill Cornell Medical College, Cornell University, New York, NY, USA.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Liu
authors.affiliation: Aerosol and Particle Technology Laboratory, CPERI/CERTH, Thessaloniki, Greece.
authors.forename: Akrivi
authors.initials: A
authors.lastname: Asimakopoulou
authors.affiliation: Aerosol and Particle Technology Laboratory, CPERI/CERTH, Thessaloniki, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Papaioannou
authors.affiliation: Aerosol and Particle Technology Laboratory, CPERI/CERTH, Thessaloniki, Greece.|Department of Chemical Engineering, Aristotle University, Thessaloniki, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Konstandopoulos
authors.affiliation: Institute for Work and Health (IST), University of Lausanne, Lausanne, Switzerland.|SAFENANO, IOM Singapore, Singapore, Singapore.
authors.forename: Michael
authors.initials: M
authors.lastname: Riediker
mesh_terms: D000336:Aerosols; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival; D002478:Cells, Cultured; D004339:Drug Compounding; D016503:Drug Delivery Systems; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050997:Nanomedicine; D053758:Nanoparticles; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
chemical_list: D000336:Aerosols; D005290:Ferric Compounds; D017382:Reactive Oxygen Species; C000499:ferric oxide; D007770:L-Lactate Dehydrogenase
keywords: Aerosol; Air-interfaced culture; Iron oxide; Nanoparticles; SPIONs