title: Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney.
abstract: BACKGROUND
  A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A. oxyphylla extract (AOE) in a type II diabetic animal model (C57BIKsj db-/db-).
  
  
  RESULTS
  Treatment with AOE for 8 weeks led to lower concentrations of blood glucose, urine albumin, and urine creatinine. 17 and 13 miRNAs were statistically identified as differentially regulated in the DB/DB and db-/db- AOE mice, respectively, compared to the untreated db-/db- mice. Of these, 7 miRNAs were identified in both comparison groups, and these 7 miRNAs were verified by quantitative real-time PCR. Functional bioinformatics showed that the putative target genes of 7 miRNAs were associated with several diabetes effects and signaling pathways.
  
  
  CONCLUSIONS
  These founding suggest that the potential of AOE as a medicinal anti-diabetes treatment through changes in the expressions of specific miRNAs. The results provide a useful resource for future investigation of the role of AOE-regulated miRNAs in diabetes mellitus.
authors.affiliation: Department of Biochemistry and Molecular Biology, Hainan Medical College, Haikou, 571101, China.
authors.forename: Guankui
authors.initials: G
authors.lastname: Du
authors.affiliation: Department of Biochemistry and Molecular Biology, Hainan Medical College, Haikou, 571101, China.
authors.forename: Man
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Department of Biochemistry and Molecular Biology, Hainan Medical College, Haikou, 571101, China.
authors.forename: Xuezi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Hainan Medical College, Haikou, 571101, China.
authors.forename: Maoyu
authors.initials: M
authors.lastname: Wen
authors.affiliation: Department of Biochemistry and Molecular Biology, Hainan Medical College, Haikou, 571101, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Pang
authors.affiliation: Department of Biochemistry and Molecular Biology, Hainan Medical College, Haikou, 571101, China.
authors.forename: Shangfei
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Affiliated Hospital of Hainan Medical College, Haikou, 571199, China. lytssg@126.com.|Clinical Laboratory, Hainan Medical College Affiliated Hospital, Haikou, 571199, China. lytssg@126.com.
authors.forename: Shenggang
authors.initials: S
authors.lastname: Sang
authors.affiliation: Affiliated Hospital of Hainan Medical College, Haikou, 571199, China. xieyiqiang_hy@163.com.|College of Chinese Traditional Medicine, Hainan Medical College, Haikou, 571199, China. xieyiqiang_hy@163.com.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000419:Albuminuria; D032429:Alpinia; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003404:Creatinine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000188:drug therapy / Q000378:metabolism; D005786:Gene Expression Regulation; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: C442779:Alpinia oxyphylla fruit extract; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D035683:MicroRNAs; D010936:Plant Extracts; D003404:Creatinine
keywords: Alpinia oxyphylla Miq; Diabetic nephropathy; Kidney; db-/db- mice; miRNA