title: Sodium Selenite Prevents Paraquat-Induced Neurotoxicity in Zebrafish.
abstract: Considering the antioxidant properties of sodium selenite (Na2SeO3) and the involvement of oxidative stress events in paraquat-induced neurotoxicity, this study investigated the protective effect of dietary Na2SeO3on biochemical and behavioral parameters of zebrafish exposed to paraquat (PQ). Fish were pretreated with a Na2SeO3diet for 21 days and then PQ (20 mg/kg) was administered intraperitoneally with six injections for 16 days. In the novel tank test, the Na2SeO3diet prevented the locomotor impairments, as well as the increase in the time spent in the top area of the tank, and the exacerbation of freezing episodes. In the preference for conspecifics and in the mirror-induced aggression (MIA) tasks, Na2SeO3prevented the increase in the latency to enter the area closer to conspecifics and the agonistic behavior of PQ-treated animals, respectively. Na2SeO3prevented the increase of carbonylated protein (CP), reactive oxygen species (ROS), and nitrite/nitrate (NOx) levels, as well as the decrease in non-protein thiols (NPSH) levels. Regarding the antioxidant enzymatic defenses, Na2SeO3prevented the increase in catalase (CAT) and glutathione peroxidase (GPx) activities caused by PQ. Altogether, dietary Na2SeO3improves behavioral and biochemical function impaired by PQ treatment in zebrafish, by modulating not only redox parameters, but also anxiety- and aggressive-like phenotypes in zebrafish.
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Talise E
authors.initials: TE
authors.lastname: Müller
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Mauro E
authors.initials: ME
authors.lastname: Nunes
authors.affiliation: Graduate Program in Animal Biodiversity, Department of Molecular Biology and Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Charlene C
authors.initials: CC
authors.lastname: Menezes
authors.affiliation: Graduate Program in Animal Biodiversity, Department of Molecular Biology and Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Aline T
authors.initials: AT
authors.lastname: Marins
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Jossiele
authors.initials: J
authors.lastname: Leitemperger
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Ana Carolina Lopes
authors.initials: ACL
authors.lastname: Gressler
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Fabiano B
authors.initials: FB
authors.lastname: Carvalho
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Catiuscia Molz
authors.initials: CM
authors.lastname: de Freitas
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Vanessa A
authors.initials: VA
authors.lastname: Quadros
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Roselei
authors.initials: R
authors.lastname: Fachinetto
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.|The International Zebrafish Neuroscience Research Consortium (ZNRC), 309 Palmer Court, Slidell, LA, 70458, USA.
authors.forename: Denis B
authors.initials: DB
authors.lastname: Rosemberg
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil. vania.loro@gmail.com.|Graduate Program in Animal Biodiversity, Department of Molecular Biology and Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS, 97105-900, Brazil. vania.loro@gmail.com.
authors.forename: Vania L
authors.initials: VL
authors.lastname: Loro
mesh_terms: D000818:Animals; D006540:Herbicides / Q000633:toxicity*; D008124:Locomotion / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010269:Paraquat / Q000633:toxicity*; D018038:Sodium Selenite / Q000008:administration & dosage*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015027:Zebrafish
chemical_list: D006540:Herbicides; D018696:Neuroprotective Agents; D017392:Thiobarbituric Acid Reactive Substances; D018038:Sodium Selenite; D010269:Paraquat
keywords: Behavior; Oxidative stress; Parkinson’s disease; Selenium; Zebrafish