title: Ascorbic acid reduces redox potential in human spermatozoa subjected to heat-induced oxidative stress.
abstract: Oxidation-reduction potential (ORP) is a new measure of oxidative stress. It is a balance between the total available oxidants and reductants. This study measures the efficiency of ascorbic acid (AA) against oxidative stress induced by either heat alone or heat and hydrogen peroxide in sperm suspensions using the MiOXSYS System. Two concentrations of ascorbic acid (400 and 600 μmol/L) were tested against heat- and heat plus hydrogen peroxide-induced oxidative stress in sperm suspensions after 2 and 4 hr of incubation. Sperm motility and static oxidation reduction potential (sORP) were measured at 2 and 4 hr of incubation at three different temperatures. A significant decrease in sORP was observed as a function of AA concentration. The 600 μmol/L AA had more pronounced reduction in sORP compared to 400 μmol/L AA (p = .001). Significant decreases in sperm motility ranging from 4.89% to 14.02% were observed both as a function of incubation time and addition of H2O2(p < .001). Ascorbic acid is efficacious to reduce heat-induced oxidative stress in sperm preparations in vitro. The supplementation of ascorbic acid may be advantageous for semen preparations in IUI, IVF and ICSI.
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.|Department of Physiology, University of Health Sciences, Lahore, Pakistan.|College of Medicine, Prince Sattam Bin Abdulaziz University, Al Kharj, KSA, Saudi Arabia.
authors.forename: G
authors.initials: G
authors.lastname: Ahmad
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: A
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: ANDROFERT, Andrology and Human Reproduction Clinic, Campinas, SP, Brazil.
authors.forename: S C
authors.initials: SC
authors.lastname: Esteves
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sharma
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: M
authors.initials: M
authors.lastname: Almasry
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: A
authors.initials: A
authors.lastname: Al-Gonaim
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: G
authors.initials: G
authors.lastname: AlHayaza
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: N
authors.initials: N
authors.lastname: Singh
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: L
authors.initials: L
authors.lastname: Al Kattan
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: W M
authors.initials: WM
authors.lastname: Sannaa
authors.affiliation: Department of Urology, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: E
authors.initials: E
authors.lastname: Sabanegh
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: ascorbic acid; heat; hydrogen peroxide; oxidation-reduction potential; spermatozoa