title: Effect of Ageratina adenophora invasion on the composition and diversity of soil microbiome.
abstract: In the present study, high throughput 16S rRNA gene sequencing was used to investigate soil invaded by the aggressive weed Ageratina adenophora to determine its effect on the species composition, distribution, and biodiversity of the bacterial communities. Soil samples from 12 micro-sites containing a monoculture of A. adenophora plants, mixtures of A. adenophora and different native plant species, and native species alone were studied. We found that the invasion of this weed resulted in a selection of bacteria belonging to phyla Acidobacteria and Verrucomicrobia and the lack of bacteria belonging to phyla Actinobacteria and Planctomycetes, but did not affect significantly the percentage abundances of members of other phyla. A similar bacterial population selection was also observed at genus or subgroup levels. The NO3--N level was an important factor affecting soil bacterial communities and contributed to the dominance of A. adenophora. However, the numbers of total bacterial species, and the diversity and structure of soil bacterial microbiome did not (P > 0.05) change significantly following invasion by this weed.
authors.affiliation: Key Laboratory of Special Biological Resource Development and Utilization of Universities of Yunnan Province, Kunming University.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Department of Computer Science, Waterloo University.
authors.forename: James
authors.initials: J
authors.lastname: Kong
authors.affiliation: Key Laboratory of Special Biological Resource Development and Utilization of Universities of Yunnan Province, Kunming University.
authors.forename: Dingkang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Agricultural School, Kunming University.
authors.forename: Heping
authors.initials: H
authors.lastname: Huang
authors.affiliation: Agricultural School, Kunming University.
authors.forename: Kaiyou
authors.initials: K
authors.lastname: Geng
authors.affiliation: Microbiology Institute of Yunnan University.
authors.forename: Yonxia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Agricultural School, Kunming University.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xia
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D036603:Ageratina / Q000254:growth & development / Q000382:microbiology*; D002681:China; D059014:High-Throughput Nucleotide Sequencing; D064307:Microbiota; D061328:Planctomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D057685:Plant Weeds / Q000254:growth & development / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: Ageratina adenophora; high throughput sequencing; undersurface soil microbiome; weed invasion