title: Structure and Function of a Novel ATPase that Interacts with Holliday Junction Resolvase Hjc and Promotes Branch Migration.
abstract: Holliday junction (HJ) is a hallmark intermediate in DNA recombination and must be processed by dissolution (for double HJ) or resolution to ensure genome stability. Although HJ resolvases have been identified in all domains of life, there is a long-standing effort to search in prokaryotes and eukarya for proteins promoting HJ migration. Here, we report the structural and functional characterization of a novel ATPase, Sulfolobus islandicusPilT N-terminal-domain-containing ATPase (SisPINA), encoded by the gene adjacent to the resolvase Hjc coding gene. PINA is conserved in archaea and vital for S. islandicus viability. Purified SisPINA forms hexameric rings in the crystalline state and in solution, similar to the HJ migration helicase RuvB in Gram-negative bacteria. Structural analysis suggests that ATP binding and hydrolysis cause conformational changes in SisPINA to drive branch migration. Further studies reveal that SisPINA interacts with SisHjc and coordinates HJ migration and cleavage.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan 250100, PR China.
authors.forename: Binyuan
authors.initials: B
authors.lastname: Zhai
authors.affiliation: Department of Biochemistry, University of California, Riverside, CA 92521, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: DuPrez
authors.affiliation: Macromolecular Crystallography Group, Stanford Synchrotron Radiation Light Source, SLAC National Accelerator Laboratory, Stanford University, Stanford, CA 94309, USA.
authors.forename: Tzanko I
authors.initials: TI
authors.lastname: Doukov
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan 250100, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan 250100, PR China.
authors.forename: Mengting
authors.initials: M
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan 250100, PR China.
authors.forename: Guijun
authors.initials: G
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan 250100, PR China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan 250100, PR China.
authors.forename: Lichuan
authors.initials: L
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Road, Jinan 250100, PR China. Electronic address: yulgshen@sdu.edu.cn.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Biochemistry, University of California, Riverside, CA 92521, USA. Electronic address: lifan@ucr.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002470:Cell Survival; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D045565:Holliday Junction Resolvases / Q000378:metabolism*; D006868:Hydrolysis; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D016944:Sulfolobus / Q000201:enzymology* / Q000502:physiology
chemical_list: D000255:Adenosine Triphosphate; D004247:DNA; D045565:Holliday Junction Resolvases; D000251:Adenosine Triphosphatases
keywords: ATPase; DNA recombinational repair; Holliday junction migration; Sulfolobus islandicus; archaea