title: Regulation of Scaffold Cell Adhesion Using Artificial Membrane Binding Proteins.
abstract: The rapid pace of development in biotechnology has placed great importance on controlling cell-material interactions. In practice, this involves attempting to decouple the contributions from adhesion molecules, cell membrane receptors, and scaffold surface chemistry and morphology, which is extremely challenging. Accordingly, a strategy is presented in which different chemical, biochemical, and morphological properties of 3D biomaterials are systematically varied to produce novel scaffolds with tuneable cell affinities. Specifically, cationized and surfactant-conjugated proteins, recently shown to have non-native membrane affinity, are covalently attached to 3D scaffolds of collagen or carboxymethyl-dextran, yielding surface-functionalized 3D architectures with predictable cell immobilization profiles. The artificial membrane-binding proteins enhance cellular adhesion of human mesenchymal stem cells (hMSCs) via electrostatic and hydrophobic binding mechanisms. Furthermore, functionalizing the 3D scaffolds with cationized or surfactant-conjugated myoglobin prevents a slowdown in proliferation of seeded hMSCs cultured for seven days under hypoxic conditions.
authors.affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.|Centre for Organized Matter Chemistry and Centre for Protolife Research, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.|Bristol Centre for Functional Nanomaterials, University of Bristol, Bristol, BS8 1FD, UK.
authors.forename: Madeline
authors.initials: M
authors.lastname: Burke
authors.affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.
authors.forename: James P K
authors.initials: JPK
authors.lastname: Armstrong
authors.affiliation: Centre for Organized Matter Chemistry and Centre for Protolife Research, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Goodwin
authors.affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Deller
authors.affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Carter
authors.affiliation: Chemical Imaging Facility, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Harniman
authors.affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.
authors.forename: Aasiya
authors.initials: A
authors.lastname: Ginwalla
authors.affiliation: Department of Mechanical Engineering, University of Bristol, Bristol, BS8 1TS, UK.
authors.forename: Valeska P
authors.initials: VP
authors.lastname: Ting
authors.affiliation: Centre for Organized Matter Chemistry and Centre for Protolife Research, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.|Chemical Imaging Facility, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
authors.forename: Sean A
authors.initials: SA
authors.lastname: Davis
authors.affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.|Centre for Organized Matter Chemistry and Centre for Protolife Research, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
authors.forename: Adam W
authors.initials: AW
authors.lastname: Perriman
mesh_terms: D002448:Cell Adhesion; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003911:Dextrans; D003094:Collagen; C014392:carboxymethyl dextran
keywords: cellular adhesion; hypoxia; myoglobin; scaffolds; stem cells