title: Comparison of FFPE histological versus LBP cytological samples for HPV detection and typing in cervical cancer.
abstract: Human papillomavirus (HPV) infection is closely associated with cervical cancer. This study analyzed HPV genotype prevalence in 75 cases of formalin-fixed paraffin embedded (FFPE) tissue samples from patients diagnosed with cervical cancer. Genotype prevalence was assessed using Reverse Blot Assay (REBA) and quantitative polymerase chain reaction (qPCR), which target the HPV L1 and HPV E6/E7 genes, respectively. HPV DNA chip tests were also performed using liquid based preparation (LBP) cytological samples from the same patients who provided the FFPE histological samples. We observed a slight difference in HPV genotype distribution as assessed by DNA chip versus REBA. One possible explanation for this difference is that normal regions could be mixed with lesion regions when cytological samples are extracted from each patient with cancer. For the detection of moderate dysplasia, the main target of diagnosis, this difference is anticipated to be greater. We also made several unexpected observations. For example, HPV multi-infection was not detected. Moreover, the rate of HPV positivity varied radically depending on the cancer origin, e.g. squamous cell carcinoma versus adenocarcinoma. Our results imply that it is important to determine whether cytological specimens are suitable for HPV genotyping analysis and cervical cancer diagnosis. Future research on the mechanisms underlying cervical cancer pathogenesis is also necessary.
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of Korea.
authors.forename: Geehyuk
authors.initials: G
authors.lastname: Kim
authors.affiliation: Wonju Severance Christian Hospital, Wonju, Gangwon Province, Republic of Korea.
authors.forename: Hyemi
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Clinical Laboratory Science, Hyejeon College, Hongseong, Chungnam Province, Republic of Korea.
authors.forename: Dongsup
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of Korea.
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.affiliation: M&D, Inc., Wonju Eco Environmental Technology Center, Wonju, Gangwon Province, Republic of Korea.
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan, Republic of Korea.
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Pathology, Yonsei University, Wonju College of Medicine, Wonju, Gangwon Province, Republic of Korea.
authors.forename: Kwang Hwa
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of Korea. Electronic address: hyelee@yonsei.ac.kr.
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005557:Formaldehyde / Q000737:chemistry*; D005814:Genes, Viral; D060005:Genotyping Techniques; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D013048:Specimen Handling; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000821:virology
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D005557:Formaldehyde
keywords: Cervical cancer; FFPE; HPV; qPCR