title: Long non-coding RNA XIST promotes cell growth by regulating miR-139-5p/PDK1/AKT axis in hepatocellular carcinoma.
abstract: Abnormal expression of long non-coding RNA often contributes to unrestricted growth of cancer cells. Long non-coding RNA XIST expression is upregulated in several cancers; however, its modulatory mechanisms have not been reported in hepatocellular carcinoma. In this study, we found that XIST expression was significantly increased in hepatocellular carcinoma tissues and cell lines. XIST promoted cell cycle progression from the G1 phase to the S phase and protected cells from apoptosis, which contributed to hepatocellular carcinoma cell growth. In addition, we revealed that there was reciprocal repression between XIST and miR-139-5p. PDK1 was identified as a direct target of miR-139-5p. We proposed that XIST was responsible for hepatocellular carcinoma cell proliferation, and XIST exerted its function through the miR-139-5p/PDK1 axis.
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Mo
authors.affiliation: 2 Department of Radiation Oncology, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Yaoyong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: 3 Macau University of Science and Technology, Taipa, Macau.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: 4 Graduate School of Guangdong Medical College, Zhanjiang, China.
authors.forename: Simin
authors.initials: S
authors.lastname: Huang
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Longguang
authors.initials: L
authors.lastname: He
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Dasheng
authors.initials: D
authors.lastname: Li
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Fuliang
authors.initials: F
authors.lastname: Li
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Huang
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Lin
authors.affiliation: 5 Department of Obstetrics and Gynecology, Shenzhen Nanshan Hospital, Shenzhen, China.
authors.forename: Xueru
authors.initials: X
authors.lastname: Li
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Siyao
authors.initials: S
authors.lastname: Che
authors.affiliation: 1 Department of Hepatobiliary Surgery, Gaozhou People's Hospital, Gaozhou, China.
authors.forename: Qinshou
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: C555098:MIRN139 microRNA, human; D035683:MicroRNAs; C000631429:PDK1 protein, human; C000631433:Pdk1 protein, mouse; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: PDK1; XIST; hepatocellular carcinoma; miR-139-5p