title: Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development.
abstract: To elucidate metabolism of ascorbic acid (AsA) in sweet cherry fruit (Prunus avium 'Hongdeng'), we quantified AsA concentration, cloned sequences involved in AsA metabolism and investigated their mRNA expression levels, and determined the activity levels of selected enzymes during fruit development and maturation. We found that AsA concentration was highest at the petal-fall period (0 days after anthesis) and decreased progressively during ripening, but with a slight increase at maturity. AsA did nevertheless continue to accumulate over time because of the increase in fruit fresh weight. Full-length cDNAs of 10 genes involved in the L-galactose pathway of AsA biosynthesis and 10 involved in recycling were obtained. Gene expression patterns of GDP-L-galactose phosphorylase (GGP2), L-galactono-1, 4-lactone dehydrogenase (GalLDH), ascorbate peroxidase (APX3), ascorbate oxidase (AO2), glutathione reductase (GR1), and dehydroascorbate reductase (DHAR1) were in accordance with the AsA concentration pattern during fruit development, indicating that genes involved in ascorbic acid biosynthesis, degradation, and recycling worked in concert to regulate ascorbic acid accumulation in sweet cherry fruit.
authors.affiliation: Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.affiliation: College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhu
authors.affiliation: College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Ni
authors.affiliation: Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Lijin
authors.initials: L
authors.lastname: Lin
authors.affiliation: Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Lv
authors.affiliation: Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
mesh_terms: D001204:Ascorbate Oxidase / Q000235:genetics / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000235:genetics / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005980:Glutathione Reductase / Q000235:genetics / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D000068220:Prunus avium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D001204:Ascorbate Oxidase; D060387:Ascorbate Peroxidases; D005980:Glutathione Reductase; D010744:Phosphoric Monoester Hydrolases; D001205:Ascorbic Acid