title: Comparative pharmacokinetics of bergenin, a main active constituent of Saxifraga stolonifera Curt., in normal and hepatic injury rats after oral administration.
abstract: Bergenin, isolated from the herb of Saxifrage stolonifera Curt. (Hu-Er-Cao) has hepatoprotective, anti-inflammatory, antitussive, and neuroprotective activities. The aim of the present study was to establish a simple, rapid, and sensitive RP-HPLC method for determination of bergenin in rat plasma and compare its oral pharmacokinetic behaviors in normal and CCl4-induced hepatic injury rats. With norisoboldine as an internal standard, chromatographic separation was performed on a C18analytical column with acetonitrile and water (11 : 89, V/V) containing 0.1% formic acid as the mobile phase. A good linearity was obtained over the range of 100-10 000 ng·mL-1. The lower limit of quantification was 50 ng·mL-1. The developed method was successfully applied to a study of the pharmacokinetic difference of bergenin (100 mg·kg-1) between normal and hepatic injury rats after oral administration. Marked alterations of pharmacokinetic parameters in hepatic injury rats were observed. Compared to normal rats, the AUC(0-∞)of bergenin in hepatic injury rats was elevated to 2.11-fold and Cmaxwas increased by 130%, whereas CL value was only 55% of the normal rats, suggesting that the systemic exposure of bergenin was significantly increased under hepatic injury status.
authors.affiliation: The Chinese Traditional Medical Hospital of Liyang City, Liyang 213300, China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Pan
authors.affiliation: Department of Chinese Materia Medica Analysis, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: He
authors.affiliation: Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Chinese Materia Medica Analysis, China Pharmaceutical University, Nanjing 210009, China. Electronic address: yfxiacpu@126.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Xia
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D031995:Saxifragaceae / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001578:Benzopyrans; D004365:Drugs, Chinese Herbal; D002251:Carbon Tetrachloride; C006741:bergenin
keywords: Bergenin; HPLC; Hepatic injury; Pharmacokinetic; Saxifrage stolonifera Curt