title: Comparative "Golgi" Proteome Study of Lolium multiflorum and Populus trichocarpa.
abstract: The Golgi apparatus (GA) is a crucial organelle in the biosynthesis of non-cellulosic polysaccharides, glycoproteins and proteoglycans that are primarily destined for secretion to the cell surface (plasma membrane, cell wall and apoplast). Only a small proportion of the proteins involved in these processes have been identified in plants, with the majority of their functions still unknown. The availability of a GA proteome would greatly assist plant biochemists, cell and molecular biologists in determining the precise function of the cell wall-related proteins. There has been some progress towards defining the GA proteome in the model plant systemArabidopsis thaliana, yet in commercially important species, such as either the cereals or woody species there has been relatively less progress. In this study, we applied discontinuous sucrose gradient centrifugation to partially enrich GA from suspension cell cultures (SCCs) and combined this with stable isotope labelling (iTRAQ) to determine protein sub-cellular locations. Results from a representative grass species, Italian ryegrass (Lolium multiflorum) and a dicot species, black cottonwood (Populus trichocarpa) are compared. The results confirm that membrane fractionation approaches that provide effective GA-enriched fractions for proteomic analyses inArabidopsisare much less effective in the species examined here and highlight the complexity of the GA, both within and between species.
authors.affiliation: Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia. kferg@unimelb.edu.au.
authors.forename: Kristina L
authors.initials: KL
authors.lastname: Ford
authors.affiliation: Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia. tonyccm@gmail.com.
authors.forename: Tony
authors.initials: T
authors.lastname: Chin
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden. vasri@kth.se.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Srivastava
authors.affiliation: Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia. zengw@unimelb.edu.au.
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia. msdoblin@unimelb.edu.au.
authors.forename: Monika S
authors.initials: MS
authors.lastname: Doblin
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden. bulone@kth.se.|Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia. bulone@kth.se.
authors.forename: Vincent
authors.initials: V
authors.lastname: Bulone
authors.affiliation: Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia. abacic@unimelb.edu.au.
authors.forename: Antony
authors.initials: A
authors.lastname: Bacic
keywords: Golgi apparatus; quantitative proteomics; sub-cellular fractionation; subcellular proteomics