title: Genomic variability ofHelicobacter pyloriisolates of gastric regions from two Colombian populations.
abstract: OBJECTIVE
  To compare the genomic variability and the multiple colonization ofHelicobacter pylori(H. pylori) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer (GC): Túquerres-Nariño (High risk) and Tumaco-Nariño (Low risk).
  
  
  METHODS
  Four hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whomH. pyloriwas isolated from three anatomic regions of the gastric mucosa, (31/206) of the high risk population of GC (Túquerres) and (41/203) of the low risk population of GC (Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence analyses.
  
  
  RESULTS
  The proportion of virulent genotypes ofH. pyloriwas 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversity among isolates of Túquerres (0.13) than those of Tumaco (0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco (P= 0.05).
  
  
  CONCLUSIONS
  In Túquerres, high risk of GC there was a greater probability of multiple colonization byH. pylori. From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates ofH. pyloriin the population of high risk for the development of GC.
authors.affiliation: Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia.
authors.forename: Andrés Jenuer
authors.initials: AJ
authors.lastname: Matta
authors.affiliation: Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia.
authors.forename: Alvaro Jairo
authors.initials: AJ
authors.lastname: Pazos
authors.affiliation: Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia.
authors.forename: Javier Andrés
authors.initials: JA
authors.lastname: Bustamante-Rengifo
authors.affiliation: Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia.
authors.forename: Luis Eduardo
authors.initials: LE
authors.lastname: Bravo
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D003105:Colombia / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005756:Gastritis / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D005798:Genes, Bacterial; D014644:Genetic Variation; D016481:Helicobacter Infections / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D012307:Risk Factors; D013270:Stomach / Q000382:microbiology; D013274:Stomach Neoplasms / Q000209:etiology; D014774:Virulence / Q000235:genetics; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori
keywords: Helicobacter pylori; Multiple colonization; PCR-RAPD; Pathogenicity islet cag; cagA; vacA