title: Purification, characterization and procoagulant activity of polysaccharides fromAngelica dahuriceroots.
abstract: Five polysaccharides, namely ADPs-1a, ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b, were extracted fromAngelicae dahuricae Radix, purified, and identified by high performance gel permeation chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance spectra (NMR), including the determination of procoagulant activity in vitro. The average molecular weight (Mw) of the polysaccharides was 153,800, 8312, 111,700, 3766 and 96,680 g/mol, respectively. Coagulation assays indicated that ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b had procoagulant activities. ADPs-1b exerted the procoagulant activities through intrinsic pathway, extrinsic pathway and increased the content of FIB in vitro. ADPs-2 exerted the procoagulant activities through intrinsic pathway and extrinsic pathway. ADPs-3a had procoagulant activities and the activity was associated with the intrinsic pathway and increased the content of FIB. ADPs-3b exerted the activities through extrinsic pathway and increased the content of FIB.
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.|Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, 475004 China.
authors.forename: Pengli
authors.initials: P
authors.lastname: Lian
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.|Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, 475004 China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wei
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.|Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, 475004 China.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Kang
keywords: Angelicae dahuricae Radix; Polysaccharides; Procoagulant