title: A reaction-based near-infrared fluorescent sensor for Cu2+detection in aqueous buffer and its application in living cells and tissues imaging.
abstract: Copper (II) is one of the most of important cofactors for numerous enzymes and has captured broad attention due to its role as a neurotransmitters for physiological and pathological functions. In this article, we present a reaction-based fluorescent sensor for Cu2+detection (NIR-Cu) with near-infrared excitation and emission, including probe design, structure characterization, optical property test and biological imaging application. NIR-Cu is equipped with a functional group, 2-picolinic ester, which hydrolyzes in the presence of Cu2+with high selectivity over completed cations. With the experimental conditions optimized, NIR-Cu (5μM) exhibits linear response for Cu2+range from 0.1 to 5μM, with a detection limit of 29nM. NIR-Cu also shows excellent water solubility and are highly responsive, both desirable properties for Cu2+detection in water samples. In addition, due to its near-infrared excitation and emission properties, NIR-Cu demonstrates outstanding fluorescent imaging in living cells and tissues.
authors.affiliation: School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China; Department of Physics and Materials Science, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
authors.forename: Huatang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Feng
authors.affiliation: School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
authors.forename: Yin-Ting
authors.initials: YT
authors.lastname: Wong
authors.affiliation: School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Yonghe
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Guansheng
authors.initials: G
authors.lastname: Zheng
authors.affiliation: School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China; Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China. Electronic address: hongysun@cityu.edu.hk.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China. Electronic address: derekho@cityu.edu.hk.
authors.forename: Derek
authors.initials: D
authors.lastname: Ho
mesh_terms: D015374:Biosensing Techniques; D002021:Buffers; D003300:Copper / Q000737:chemistry / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000737:chemistry; D007259:Infrared Rays; D057230:Limit of Detection; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D002021:Buffers; D005456:Fluorescent Dyes; D014867:Water; D003300:Copper
keywords: Cu(2+) detection; Fluorescent probe; Near-infrared; Tissues imaging