title: Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities.
abstract: Interlukin-10 (IL-10) is an immunomodulatory cytokine which predominantly induces immune-tolerance. It has been also identified as a major cytokine in the tumor microenvironment that markedly mediates tumor immune escape. Previous studies on the roles of IL-10 in tumor immunosuppression mainly focus on its biochemical effects. But the effects of IL-10 on the biophysical characteristics of immune cells are ill-defined. Dendritic cells (DCs) are the most potent antigen-presenting cells and play a key role in the anti-tumor immune response. IL-10 can affect the immune regulatory functions of DCs in various ways. In this study, we aim to explore the effects of IL-10 on the biophysical functions of mature DCs (mDCs). mDCs were treated with different concentrations of IL-10 and their biophysical characteristics were identified. The results showed that the biophysical properties of mDCs, including electrophoresis mobility, osmotic fragility and deformability, as well as their motilities, were impaired by IL-10. Meanwhile, the cytoskeleton (F-actin) of mDCs was reorganized by IL-10. IL-10 caused the alternations in the expressions of fasin1 and profilin1 as well as the phosphorylation of cofilin1 in a concentration-dependent fashion. Moreover, Fourier transformed infrared resonance data showed that IL-10 made the status of gene transcription and metabolic turnover of mDCs more active. These results demonstrate a new aspect of IL-10's actions on the immune system and represent one of the mechanisms for immune escape of tumors. It may provide a valuable clue to optimize and improve the efficiency of DC-based immunotherapy against cancer.
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.|Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, P.R.China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.|Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, P.R.China.
authors.forename: Xianmei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Head and Neck, Affiliated Cancer Hospital, Guizhou Medical University, Guiyang, P.R.China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Long
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Zuquan
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Qinni
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Nephropathy & Rheumatism, Third Affiliated Hospital, Guizhou Medical University, Duyun, P.R.China.
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, P.R.China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Yao
authors.affiliation: Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, P.R. China.|Engineering Center of Medical Biotechnology Application, Guizhou Medical University, Guiyang, P.R. China.|School of Biology and Engineering, Guizhou Medical University, Guiyang, P.R. China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D000199:Actins / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects*; D051338:Cofilin 1 / Q000235:genetics / Q000276:immunology; D003599:Cytoskeleton / Q000737:chemistry / Q000187:drug effects*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005786:Gene Expression Regulation; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D061251:Primary Cell Culture; D051304:Profilins / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D014158:Transcription, Genetic; D019014:fas Receptor / Q000235:genetics / Q000276:immunology
chemical_list: D000199:Actins; D051338:Cofilin 1; C491901:FAS protein, human; C508609:IL10 protein, human; C495738:PFN1 protein, human; D051304:Profilins; D011994:Recombinant Proteins; D019014:fas Receptor; D016753:Interleukin-10