title: Transcriptome analysis of Clostridium beijerinckii adaptation mechanisms in response to ferulic acid.
abstract: Clostridium beijerinckii 4693:int with high ferulic acid (FA) tolerance was engineered and characterized in our lab. In this study, the minimum inhibition concentrations of FA against C. beijerinckii NCIMB 8052 (wild-type) and 4693:int were 1.0 and 1.5g/l, respectively; cell viability was 18.5% and 106.7%, respectively, in the presence of 0.5g/l FA. A comparative transcriptome analysis was carried out at two different growth stages to evaluate sensitivity to FA. Genes that were differentially expressed included those related to redox and associated cofactors, riboflavin metabolism, two-component system, glycolysis and butanoate metabolism, and DNA replication as well as those encoding ATP-binding cassette transporters. Cbei_2134 and Cbei_2135 encoding alkyl hydroperoxide reductases are thought to be involved in antibacterial and adaptation mechanisms in C. beijerinckii in the presence of FA.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, China; National Engineering Technique Research Center for Biotechnology, Nanjing 211816, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan Province 410004, China. Electronic address: guoting@hotmail.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: College of Food Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan Province 410004, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, China; National Engineering Technique Research Center for Biotechnology, Nanjing 211816, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, China; National Engineering Technique Research Center for Biotechnology, Nanjing 211816, China.
authors.forename: Chenglun
authors.initials: C
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, China; National Engineering Technique Research Center for Biotechnology, Nanjing 211816, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, China; National Engineering Technique Research Center for Biotechnology, Nanjing 211816, China. Electronic address: yinghanjie@njtech.edu.cn.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D046970:Clostridium beijerinckii / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D003373:Coumaric Acids / Q000378:metabolism / Q000633:toxicity*; D004261:DNA Replication / Q000187:drug effects; D020869:Gene Expression Profiling; D018869:Heat-Shock Response / Q000187:drug effects / Q000235:genetics; D009154:Mutation; D010084:Oxidation-Reduction / Q000187:drug effects
chemical_list: D003373:Coumaric Acids; C004999:ferulic acid
keywords: Clostridium beijerinckii; Comparative transcriptome analysis; Ferulic acid tolerance; Metabolism pathway