title: Synthesis and application of in-situ molecularly imprinted silica monolithic in pipette-tip solid-phase microextraction for the separation and determination of gallic acid in orange juice samples.
abstract: A novel strategy was presented for the synthesis and application of functionalized silica monolithic as artificial receptor of gallic acid at micro-pipette tip. A sol-gel process was used to prepare the sorbent. In this in-situ polymerization reaction, tetraethyl orthosilicate (TEOS), 3-aminopropyl trimethoxysilane (APTMS), gallic acid and thiourea were used, respectively, as cross-linker, functionalized monomer, template and precursor to make crack-free and non-fragile structure. Such durable and inexpensive in-situ monolithic was successfully employed as useful tool for highly efficient extraction of gallic acid from orange juice samples. The effective parameters in extraction recovery were investigated and optimum conditions were obtained using experimental design methodology. Applying HPLC-UV for separation quantification at optimal conditions, the gallic acid was efficiently extracted without significant matrix interference. Good linearity for gallic acid in the range of 0.02-5.0mgL-1with correlation coefficients of R2>0.999 revealed well applicability of the method for trace analysis.
authors.affiliation: Chemistry Department, Yasouj University, Yasouj, 75914-35, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Arabi
authors.affiliation: Chemistry Department, Yasouj University, Yasouj, 75914-35, Iran. Electronic address: m_ghaedi@mail.yu.ac.ir.
authors.forename: Mehrorang
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Ostovan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry; D005504:Food Analysis / Q000379:methods; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D005707:Gallic Acid / Q000032:analysis / Q000302:isolation & purification*; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D058105:Polymerization; D011437:Propylamines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D011437:Propylamines; D012821:Silanes; C088294:3-aminopropyltrimethoxysilane; C040733:tetraethoxysilane; D005707:Gallic Acid; D012822:Silicon Dioxide
keywords: Experimental design; Gallic acid; HPLC-UV; Molecularly imprinted; Monolithic; Pipette-tip solid phase microextraction