title: Intraspecific variation among Tetranychid mites for ability to detoxify and to induce plant defenses.
abstract: Two genotypes coexist among Kanzawa spider mites, one of which causes red scars and the other of which causes white scars on leaves, and they elicit different defense responses in host plants. Based on RNA-Seq analysis, we revealed here that the expression levels of genes involved in the detoxification system were higher in Red strains than White strains. The corresponding enzyme activities as well as performances for acaricide resistance and host adaptation toward Laminaceae were also higher in Red strains than White strains, indicating that Red strains were superior in trait(s) of the detox system. In subsequent generations of strains that had survived exposure to fenpyroximate, both strains showed similar resistance to this acaricide, as well as similar detoxification activities. The endogenous levels of salicylic acid and jasmonic acid were increased similarly in bean leaves damaged by original Red strains and their subsequent generations that inherited high detox activity. Jasmonic acid levels were increased in leaves damaged by original White strains, but not by their subsequent generations that inherited high detox activity. Together, these data suggest the existence of intraspecific variation - at least within White strains - with respect to their capacity to withstand acaricides and host plant defenses.
authors.affiliation: Center for Ecological Research, Kyoto University, Otsu 520-2113, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Ozawa
authors.affiliation: Department of Biological Science &Technology, Faculty of Industrial Science &Technology, Tokyo University of Science, Tokyo 125-8585, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Endo
authors.affiliation: Department of Biological Science &Technology, Faculty of Industrial Science &Technology, Tokyo University of Science, Tokyo 125-8585, Japan.
authors.forename: Mei
authors.initials: M
authors.lastname: Iijima
authors.affiliation: Center for Ecological Research, Kyoto University, Otsu 520-2113, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Center for Ecological Research, Kyoto University, Otsu 520-2113, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Takabayashi
authors.affiliation: Laboratory of Applied Entomology and Zoology, Faculty of Agriculture, Ibaraki University, Ibaraki 300-0393, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Gotoh
authors.affiliation: Department of Biological Science &Technology, Faculty of Industrial Science &Technology, Tokyo University of Science, Tokyo 125-8585, Japan.
authors.forename: Gen-Ichiro
authors.initials: GI
authors.lastname: Arimura
mesh_terms: D056810:Acaricides / Q000378:metabolism*; D000818:Animals; D001565:Benzoates / Q000378:metabolism; D003517:Cyclopentanes / Q000032:analysis; D004351:Drug Resistance; D020869:Gene Expression Profiling; D014644:Genetic Variation; D005838:Genotype; D008658:Inactivation, Metabolic; D054883:Oxylipins / Q000032:analysis; D027805:Phaseolus / Q000737:chemistry / Q000276:immunology* / Q000469:parasitology*; D018515:Plant Leaves / Q000737:chemistry / Q000276:immunology / Q000469:parasitology; D011720:Pyrazoles / Q000378:metabolism; D020156:Salicylic Acid / Q000032:analysis; D017423:Sequence Analysis, RNA; D040102:Tetranychidae / Q000235:genetics* / Q000378:metabolism
chemical_list: D056810:Acaricides; D001565:Benzoates; D003517:Cyclopentanes; D054883:Oxylipins; D011720:Pyrazoles; C011006:jasmonic acid; C415144:fenpyroximate; D020156:Salicylic Acid