title: A molecular case-control study of association of HNF1A gene polymorphisms (rs2259816 and rs7310409) with risk of coronary artery disease in Iranian patients.
abstract: BACKGROUND
  Coronary artery disease (CAD) is the one of the most common heart diseases, being the main factor of mortality and morbidity worldwide. CAD has been known as a multifactorial disease and its progression depends on genetic and environmental factors. Numerous studies have shown evidence for association between 12q24.3 locus and CAD.
  
  
  OBJECTIVE
  In the present study, the association of two HNF1A polymorphisms, rs2259816 and rs7310409, located on 12q24.3 locus with CAD was investigated in Iranian patients.
  
  
  METHODS
  Whole genomic DNA was isolated from peripheral blood obtained from 411 Iranian volunteer. Then, the polymorphisms rs2259816 and rs7310409 located on 12q24.3 locus were genotyped using TaqMan Probe Real Time PCR. Statistical analysis was performed by SPSS software, version 19.
  
  
  RESULTS
  rs2259816 (p-value = 0.006) and rs7310409 (p-value = 0.001) showed statistically significant association with CAD risk. Our results indicated that there was a significant correlation between rs2259816 and blood triglyceride. However, no correlation was found between rs2259816, rs7310409 and other CAD risk factors.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Samaneh Adami
authors.initials: SA
authors.lastname: Barzi
authors.affiliation: Urogenital Stem Cell Research, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Sayyed Mohammad Hossein
authors.initials: SM
authors.lastname: Ghaderian
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Noormohammadi
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D002881:Chromosomes, Human, Pair 12; D003324:Coronary Artery Disease / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000097:blood / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D014280:Triglycerides / Q000097:blood
chemical_list: C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D014280:Triglycerides
keywords: Coronary artery disease; polymorphism; taqMan