title: Effects of Cymodocea nodosa extract on metabolic disorders and oxidative stress in alloxan-diabetic rats.
abstract: This new study aimed to evaluate for the first time the effect of Cymodocea nodosa extract (CNE) on α-amylase activity, hyperglycemia and diabetes complications in the alloxan-induced diabetic rats. The in vitro evaluation and oral administration of CNE to surviving diabetic rats inhibited key enzyme related to hyperglycemia as α-amylase, helped to protect the β cells of the rats from death and damage confirmed by oral glucose test tolerance (OGTT), which leads to decrease in blood glucose level by 49% as compared to untreated diabetic rats. The CNE also decreased the triglyceride, low density lipoprotein (LDL) cholesterol and total cholesterol rates in the plasma of diabetic rats by 46%, 35%, and 21%, respectively, and increased the high density lipoprotein (HDL) cholesterol level by 36%, which helped maintain the homeostasis of blood lipid. When compared to those of the untreated diabetic rats, the superoxide dismutase, catalase, and glutathione peroxidase levels in the pancreas, liver and kidney of the rats treated with this supplement were also enhanced significantly. Moreover, a significant decrease was observed in the lipid peroxidation level in the tested organs of diabetic rats after CNE administration. This positive effect of CNE was confirmed by histological study. Overall, the findings presented in this study demonstrate that CNE has both a promising potential with a valuable hypoglycemic and hypolipidemic functions.
authors.affiliation: Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, 3038 Sfax, Tunisia. Electronic address: rihab_b86@hotmail.com.
authors.forename: Rihab
authors.initials: R
authors.lastname: Ben Abdallah Kolsi
authors.affiliation: Laboratory of Chemistry of Natural Substances, Faculty of Sciences of Sfax, PB 802, 3018 Sfax, Tunisia.
authors.forename: Hichem
authors.initials: H
authors.lastname: Ben Salah
authors.affiliation: Histology, Orthopaedic and Traumatology Laboratory Sfax Faculty of Medicine, Sfax, Tunisia.
authors.forename: Neila
authors.initials: N
authors.lastname: Jardak
authors.affiliation: Biochemistry Laboratory, CHU Hedi Chaker, Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Chaaben
authors.affiliation: Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.affiliation: Histology, Orthopaedic and Traumatology Laboratory Sfax Faculty of Medicine, Sfax, Tunisia.
authors.forename: Tarak
authors.initials: T
authors.lastname: Rebai
authors.affiliation: Biochemistry Laboratory, CHU Hedi Chaker, Sfax, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Jamoussi
authors.affiliation: Laboratory of Chemistry of Natural Substances, Faculty of Sciences of Sfax, PB 802, 3018 Sfax, Tunisia.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Allouche
authors.affiliation: Enzyme and Bioconversion Unit, Biotechnology Center of Sfax, Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Belghith
authors.affiliation: Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, 3038 Sfax, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Belghith
mesh_terms: D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D006943:Hyperglycemia / Q000188:drug therapy / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008297:Male; D008659:Metabolic Diseases / Q000188:drug therapy* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D008055:Lipids; D010936:Plant Extracts; D014280:Triglycerides; D000496:Alloxan; D002784:Cholesterol; D000516:alpha-Amylases
keywords: Cymodoces nodosa; Diabetes complication; LC–ESI–MS/MS; α-Amylase