title: [Effects of 13-cis-retinoic acid combined with interferon-α2b in mantle cell lymphoma cell lines (Jeko-1)in vitro].
abstract: Objectives:To explore the effects of 13-cis-retinoic acid (13cRA) alone or combined with interferonα-2b (IFNα-2b) for the inhibition of cell growth and apoptosis induction of mantle cell lymphoma cell lines Jeko-1 cells.Methods:Jeko-1 cells were treated by different concentrations of 13cRA alone or combined with IFN-α2b. CCK-8 was used to measure the inhibition effects by different treatments. Cell cycles were analyzed by flow cytometry. Effects on apoptosis were assessed by staining of Annexin Ⅴ/PI. And the levels of Cyclin D1, caspase-9 and Rb proteins were measured by Western blot method.Results:13cRA alone at different doses and its combination with IFNα-2b inhibited Jeko-1 cells growth and induced apoptosis, but the combination had higher inhibition potential and significant apoptosis rate (P<0.05) . The growth inhibition and apoptosis induction in Jeko-1 cells increased significantly with the elevation of drugs concentration and treating duration (P<0.05) . As well as the percentage of Jeko-1 cells at G(1)/G(2) phases increased (P<0.05) and cells at S phase decreased (P<0.05) , the levels of Cyclin D1 and Rb decreased with elimination caspase-9.Conclusion:13cRA, IFN-α2b and their combined administration inhibited cells growth and induced apoptosis, decreased the cell populations at S phase and blocked the cells at G(1)/G(2) phase. Combination of the drugs may have a cooperated action. The therapeutic synergistic effects of 13cRA and IFN-α2b were assumed to lower the expression of Cyclin D1 and Rb proteins, and induce apoptosis by activating caspase-9 pathway.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Liu
authors.forename: J J
authors.initials: JJ
authors.lastname: Wen
authors.affiliation: Department of Hematology and Research Laboratory of Hematology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: C G
authors.initials: CG
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D053453:Caspase 9; D045744:Cell Line, Tumor; D019070:Cell Lineage; D049109:Cell Proliferation; D019938:Cyclin D1; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D015474:Isotretinoin; D020522:Lymphoma, Mantle-Cell; D011994:Recombinant Proteins
chemical_list: C530104:CCND1 protein, human; D000077190:Interferon alpha-2; D016898:Interferon-alpha; C000614170:Interferon-alpha2b; D011994:Recombinant Proteins; D019938:Cyclin D1; C506433:CASP9 protein, human; D053453:Caspase 9; D015474:Isotretinoin
keywords: Interferon-alpha; Jeko-1 cell; Lymphoma, B-cell; Tretinoin