title: Mitochondrial Cysteine Desulfurase and ISD11 Coexpressed in Escherichia coli Yield Complex Containing Acyl Carrier Protein.
abstract: Mitochondrial cysteine desulfurase is an essential component of the machinery for iron-sulfur cluster biosynthesis. It has been known that human cysteine desulfurase that is catalytically active in vitro can be prepared by overexpressing in Escherichia coli cells two protein components of this system, the cysteine desulfurase protein NFS1 and the auxiliary protein ISD11. We report here that this active preparation contains, in addition, the holo-form of E. coli acyl carrier protein (Acp). We have determined the stoichiometry of the complex to be [Acp]2:[ISD11]2:[NFS1]2. Acyl carrier protein recently has been found to be an essential component of the iron-sulfur protein biosynthesis machinery in mitochondria; thus, because of the activity of [Acp]2:[ISD11]2:[NFS1]2in supporting iron-sulfur cluster assembly in vitro, it appears that E. coli Acp can substitute for its human homologue.
authors.affiliation: Biochemistry Department, University of Wisconsin-Madison , 433 Babcock Drive, Madison, Wisconsin 53706, United States.
authors.forename: Kai
authors.initials: K
authors.lastname: Cai
authors.affiliation: Biochemistry Department, University of Wisconsin-Madison , 433 Babcock Drive, Madison, Wisconsin 53706, United States.
authors.forename: Ronnie O
authors.initials: RO
authors.lastname: Frederick
authors.affiliation: Biochemistry Department, University of Wisconsin-Madison , 433 Babcock Drive, Madison, Wisconsin 53706, United States.
authors.forename: Marco
authors.initials: M
authors.lastname: Tonelli
authors.affiliation: Biochemistry Department, University of Wisconsin-Madison , 433 Babcock Drive, Madison, Wisconsin 53706, United States.
authors.forename: John L
authors.identifier: 0000-0003-1799-6134
authors.initials: JL
authors.lastname: Markley
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism*; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D035925:Iron-Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D053838:Scattering, Small Angle; D053719:Tandem Mass Spectrometry; D014961:X-Ray Diffraction
chemical_list: D000213:Acyl Carrier Protein; D035925:Iron-Regulatory Proteins; C544445:LYRM4 protein, human; D011994:Recombinant Proteins; D013437:Carbon-Sulfur Lyases; C080546:cysteine desulfurase