title: A comparative secretome analysis of industrial Aspergillus oryzae and its spontaneous mutant ZJGS-LZ-21.
abstract: Aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. In the present study, we performed a comparative secretome analysis of the industrial strain of Aspergillus oryzae 3.042 and its spontaneous mutantZJGS-LZ-21. One hundred and fifty two (152) differential protein spots were excised (p<0.05), and 25 proteins were identified. Of the identified proteins, 91.3% belonged to hydrolytic enzymes acting on carbohydrates or proteins. Consistent with their enzyme activities, the expression of 14 proteins involved in the degradation of cellulose, hemicellulose, starch and proteins, increased in the ZJGS-LZ-21isolate. In particular, increased levels of acid protease (Pep) may favor the degradation of soy proteins in acidic environments and promote the cleavage of allergenic soybean proteins in fermentation, resulting in improvements of product safety and quality. The ZJGS-LZ-21 isolate showed higher protein secretion and increased hydrolytic activities than did strain 3.042, indicating its promising application in soybean paste fermentation.
authors.affiliation: Department of Biochemical Engineering, Zhejiang Gongshang University, Hangzhou 310025, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Biochemical Engineering, Zhejiang Gongshang University, Hangzhou 310025, China. Electronic address: dbiot@mail.zjgsu.edu.cn.
authors.forename: Xinle
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Biochemical Engineering, Zhejiang Gongshang University, Hangzhou 310025, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Zhejiang Wuweihe Food Co. Ltd, Huzhou 313213, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.affiliation: Zhejiang Wuweihe Food Co. Ltd, Huzhou 313213, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D020869:Gene Expression Profiling; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D013213:Starch
keywords: Aspergillus oryzae; Koji; Secretome; Soybean paste