title: Hyperthermia influences fate determination of neural stem cells with lncRNAs alterations in the early differentiation.
abstract: BACKGROUND
  Temperature is an important parameter in the microenvironment of neural stem cells (NSCs); however, little is known about the precise effects of hyperthermia on fate determination in NSCs or the role of long non-coding (lnc)RNAs in this process. This was addressed in the present study using NSCs cultured at two different temperatures.
  
  
  METHODS
  NSCs were divided into 37NSC and 40NSC groups that were cultured at 37°C or 40°C, respectively, for 72 h. Neuronal or glial cell differentiation was evaluated by flow cytometry and western blotting. LncRNA expression was detected by quantitative real-time PCR.
  
  
  RESULTS
  The numbers of cells positive for the neuronal marker Tuj-1 and the glial cell marker glial fibrillary acidic protein were higher in the 40NSC than in the 37NSC group. The two groups also showed distinct lncRNA expression profiles.
  
  
  CONCLUSIONS
  Hyperthermia promotes neuronal and glial differentiation in NSCs, which involves specific lncRNAs.
authors.affiliation: Department of Neurosurgery, Affiliated Haikou Hospital, Xiangya School of Central South University, Haikou, Hsinan, China.|Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
authors.forename: Da
authors.initials: D
authors.lastname: Duan
authors.affiliation: Department of Neurosurgery, Affiliated Haikou Hospital, Xiangya School of Central South University, Haikou, Hsinan, China.
authors.forename: Shuaiqi
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.|Key laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.
authors.forename: Lite
authors.initials: L
authors.lastname: Ge
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.|Key laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Affiliated Haikou Hospital, Xiangya School of Central South University, Haikou, Hsinan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D060833:Cellular Microenvironment / Q000235:genetics; D005334:Fever / Q000235:genetics*; D058953:Neural Stem Cells; D055495:Neurogenesis / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D051381:Rats; D013696:Temperature
chemical_list: D062085:RNA, Long Noncoding