title: Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients.
abstract: OBJECTIVE
  Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes.
  
  
  METHODS
  Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm).
  
  
  RESULTS
  No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations.
  
  
  CONCLUSIONS
  It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations.
authors.affiliation: Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Lobo
authors.affiliation: Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
authors.forename: Catiane Pompilio
authors.initials: CP
authors.lastname: Brescansin
authors.affiliation: Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
authors.forename: Izabella C R
authors.initials: ICR
authors.lastname: Santos-Weiss
authors.affiliation: Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
authors.forename: Marciane
authors.initials: M
authors.lastname: Welter
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, UFPR, Curitiba, PR, Brasil.
authors.forename: Emanuel Maltempi de
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
authors.forename: Fabiane Gomes de Moraes
authors.initials: FGM
authors.lastname: Rego
authors.affiliation: Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
authors.forename: Geraldo
authors.initials: G
authors.lastname: Picheth
authors.affiliation: Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
authors.forename: Dayane
authors.initials: D
authors.lastname: Alberton
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000886:Anthropometry; D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D003404:Creatinine / Q000097:blood; D016640:Diabetes, Gestational / Q000097:blood* / Q000175:diagnosis; D005260:Female; D005470:Fluorometry / Q000379:methods; D005865:Gestational Age; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D008403:Mass Screening / Q000379:methods; D011247:Pregnancy; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
chemical_list: D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D003404:Creatinine