title: Alcoholic Extract ofEclipta albaShowsIn VitroAntioxidant and Anticancer Activity without Exhibiting Toxicological Effects.
abstract: As per WHO estimates, 80% of people around the world use medicinal plants for the cure and prevention of various diseases including cancer owing to their easy availability and cost effectiveness.Eclipta albahas long been used in Ayurveda to treat liver diseases, eye ailments, and hair related disorders. The promising medicinal value ofE. albaprompted us to study the antioxidant, nontoxic, and anticancer potential of its alcoholic extract. In the current study, we evaluated thein vitrocytotoxic and antioxidant effect of the alcoholic extract ofEclipta alba(AEEA) in multiple cancer cell lines along with control. We have also evaluated its effect on differentin vivotoxicity parameters. Here, we found that AEEA was found to be most active in most of the cancer cell lines but it significantly induced apoptosis in human breast cancer cell lines by disrupting mitochondrial membrane potential and DNA damage. Moreover, AEEA treatment inhibited migration in both MCF 7 and MDA-MB-231 cells in a dose dependent manner. Further, AEEA possesses robustin vitroantioxidant activity along with high total phenolic and flavonoid contents. In summary, our results indicate thatEclipta albahas enormous potential in complementary and alternative medicine for the treatment of cancer.
authors.affiliation: Toxicology Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Navneet Kumar
authors.identifier: 0000-0003-3858-6978
authors.initials: NK
authors.lastname: Yadav
authors.affiliation: Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Arya
authors.affiliation: Botany Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Kapil
authors.initials: K
authors.lastname: Dev
authors.affiliation: Botany Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Chetan
authors.initials: C
authors.lastname: Sharma
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Zakir
authors.initials: Z
authors.lastname: Hossain
authors.affiliation: Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Meena
authors.affiliation: Botany Division, CSIR-Central Drug Research Institute, Lucknow 226031, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: K R
authors.initials: KR
authors.lastname: Arya
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow 226031, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: J R
authors.initials: JR
authors.lastname: Gayen
authors.affiliation: Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: Dipak
authors.identifier: 0000-0001-7540-8543
authors.initials: D
authors.lastname: Datta
authors.affiliation: Toxicology Division, CSIR-Central Drug Research Institute, Lucknow 226031, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: R K
authors.identifier: 0000-0003-1768-7139
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000009:adverse effects / Q000494:pharmacology*; D000975:Antioxidants / Q000009:adverse effects / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D036601:Eclipta / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005260:Female; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D061986:MCF-7 Cells; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009369:Neoplasms / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014709:Vero Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000431:Ethanol