title: Schisandra lignans production regulated by different bioreactor type.
abstract: Schisandra chinensis (Chinese magnolia vine) is a rich source of therapeutically relevant dibenzocyclooctadiene lignans with anticancer, immunostimulant and hepatoprotective activities. In this work, shoot cultures of S. chinensis were grown in different types of bioreactors with the aim to select a system suitable for the large scale in vitro production of schisandra lignans. The cultures were maintained in Murashige-Skoog (MS) medium supplemented with 3mg/l 6-benzylaminopurine (BA) and 1mg/l 1-naphthaleneacetic acid (NAA). Five bioreactors differing with respect to cultivation mode were tested: two liquid-phase systems (baloon-type bioreactor and bubble-column bioreactor with biomass immobilization), the gas-phase spray bioreactor and two commercially available temporary immersion systems: RITA®and Plantform. The experiments were run for 30 and 60 days in batch mode. The harvested shoots were evaluated for growth and lignan content determined by LC-DAD and LC-DAD-ESI-MS. Of the tested bioreactors, temporary immersion systems provided the best results with respect to biomass production and lignan accumulation: RITA®bioreactor yielded 17.86g/l (dry weight) during 60 day growth period whereas shoots grown for 30 days in Plantform bioreactor contained the highest amount of lignans (546.98mg/100g dry weight), with schisandrin, deoxyschisandrin and gomisin A as the major constituents (118.59, 77.66 and 67.86mg/100g dry weight, respectively).
authors.affiliation: Chair and Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, ul. Medyczna 9, 30-688 Kraków, Poland. Electronic address: a.szopa@uj.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szopa
authors.affiliation: Chair and Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, al. gen. J. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Kokotkiewicz
authors.affiliation: Chair and Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, al. gen. J. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Luczkiewicz
authors.affiliation: Chair and Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, ul. Medyczna 9, 30-688 Kraków, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Ekiert
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D001593:Benzyl Compounds / Q000494:pharmacology; D018533:Biomass; D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D017705:Lignans / Q000032:analysis* / Q000737:chemistry; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry / Q000254:growth & development; D011687:Purines / Q000494:pharmacology; D032141:Schisandra / Q000737:chemistry / Q000254:growth & development*
chemical_list: D001593:Benzyl Compounds; D003470:Culture Media; D017705:Lignans; D009280:Naphthaleneacetic Acids; D010936:Plant Extracts; D011687:Purines; C480551:benzylaminopurine
keywords: Deoxyschisandrin; Gomisin A; Plant bioreactors; Plantform; RITA(®); Schisandrin