title: Synthesis and screening of peptide libraries with free C-termini.
abstract: Peptide libraries are useful tools to investigate the relationship between structure and function of proteins. The creation of peptide libraries with free C-termini presents unique synthetic challenges. In this review, methods for creating peptide libraries using either solid-phase peptide synthesis or phage display are described. Methods for screening such libraries and their application in studying several important biological problems are also reported.
authors.affiliation: Departments of Chemistry and Medicinal Chemistry, University of Minnesota, Minneapolis, MN, 55455, USA.
authors.forename: Yen-Chih
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Departments of Chemistry and Medicinal Chemistry, University of Minnesota, Minneapolis, MN, 55455, USA.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Distefano
keywords: C-terminal peptide libraries; combinatorial libraries; peptide libraries; phage display; protein libraries; screening; solid-phase peptide synthesis