title: In Vitro Approaches to Model and Study Communication Between Adipose Tissue and the Liver.
abstract: Inter-organ communication is central to mammalian metabolism, allowing for the coordination of crucial homeostatic processes such as food intake, body weight, and blood glucose. Dissecting this process at a cellular and molecular level requires the establishment of a reductionist system in which cell types of interest can be investigated as the source and target of biologically relevant signals. Here, we describe a system to study the interaction between adipocytes and hepatocytes using either conditioned media from cultured adipocytes or co-culture of hepatocytes with adipose explants. These methods have the potential to identify novel polypeptides and metabolites involved in the adipose-liver metabolic axis.
authors.affiliation: Laboratory of Molecular Metabolism, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: O'Connor
authors.affiliation: Laboratory of Molecular Metabolism, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA. pcohen@rockefeller.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Cohen
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002450:Cell Communication; D002469:Cell Separation / Q000379:methods; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction
chemical_list: D017077:Culture Media, Conditioned
keywords: Adipocytes; Adipokines; Co-culture; Conditioned media; Hepatocytes; Signaling factors; Tissue explants