title: A Simple Protocol to Isolate, Characterize, and Expand Dental Pulp Stem Cells.
abstract: Adult stem cells reside in body tissues to preserve organs and whole organism homeostasis. They are acquiring a prominent role in the contemporary medicine. Many protocols to isolate and cultivate adult stem cells have been so far described, though they are often lengthy, laborious, and require very expansive instruments, materials, and reagents. On this basis, we describe a simple, cheap but at the same time functional method to: (1) isolate dental pulp stem cells (DPSC), (2) expand and cultivate DPSC, (3) cryopreserve DPSC, (4) characterize DPSC, and (5) differentiate DPSC into both mesenchymal and non-mesenchymal lineages.
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043, Orbassano (Turin), Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Di Scipio
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043, Orbassano (Turin), Italy.
authors.forename: Andrea Elio
authors.initials: AE
authors.lastname: Sprio
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043, Orbassano (Turin), Italy.
authors.forename: Maria Elisabetta
authors.initials: ME
authors.lastname: Carere
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043, Orbassano (Turin), Italy.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043, Orbassano (Turin), Italy. giovanni.berta@unito.it.
authors.forename: Giovanni Nicolao
authors.initials: GN
authors.lastname: Berta
mesh_terms: D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation; D003782:Dental Pulp / Q000166:cytology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051381:Rats
chemical_list: D015415:Biomarkers
keywords: Cell extraction; Characterization; Dental pulp stem cells; Differentiation; Expansion; Neural crest