title: Prefrontal cortex output circuits guide reward seeking through divergent cue encoding.
abstract: The prefrontal cortex is a critical neuroanatomical hub for controlling motivated behaviours across mammalian species. In addition to intra-cortical connectivity, prefrontal projection neurons innervate subcortical structures that contribute to reward-seeking behaviours, such as the ventral striatum and midline thalamus. While connectivity among these structures contributes to appetitive behaviours, how projection-specific prefrontal neurons encode reward-relevant information to guide reward seeking is unknown. Here we use in vivo two-photon calcium imaging to monitor the activity of dorsomedial prefrontal neurons in mice during an appetitive Pavlovian conditioning task. At the population level, these neurons display diverse activity patterns during the presentation of reward-predictive cues. However, recordings from prefrontal neurons with resolved projection targets reveal that individual corticostriatal neurons show response tuning to reward-predictive cues, such that excitatory cue responses are amplified across learning. By contrast, corticothalamic neurons gradually develop new, primarily inhibitory responses to reward-predictive cues across learning. Furthermore, bidirectional optogenetic manipulation of these neurons reveals that stimulation of corticostriatal neurons promotes conditioned reward-seeking behaviour after learning, while activity in corticothalamic neurons suppresses both the acquisition and expression of conditioned reward seeking. These data show how prefrontal circuitry can dynamically control reward-seeking behaviour through the opposing activities of projection-specific cell populations.
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: James M
authors.initials: JM
authors.lastname: Otis
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.|Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Vijay M K
authors.initials: VM
authors.lastname: Namboodiri
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Matan
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Elisa S
authors.initials: ES
authors.lastname: Voets
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Emily P
authors.initials: EP
authors.lastname: Mohorn
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Oksana
authors.initials: O
authors.lastname: Kosyk
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Jenna A
authors.initials: JA
authors.lastname: McHenry
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.|Neuroscience Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: J Elliott
authors.initials: JE
authors.lastname: Robinson
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Shanna L
authors.initials: SL
authors.lastname: Resendez
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Rossi
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.|Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.|Neuroscience Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.|Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Garret D
authors.initials: GD
authors.lastname: Stuber
mesh_terms: D000818:Animals; D001070:Appetitive Behavior / Q000502:physiology*; D002118:Calcium / Q000032:analysis; D003214:Conditioning, Classical / Q000502:physiology; D003463:Cues; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton; D057054:Molecular Imaging; D009434:Neural Pathways; D009473:Neuronal Plasticity; D009474:Neurons / Q000502:physiology*; D009714:Nucleus Accumbens / Q000166:cytology / Q000502:physiology; D017397:Prefrontal Cortex / Q000166:cytology* / Q000502:physiology*; D012201:Reward; D013788:Thalamus / Q000166:cytology / Q000502:physiology
chemical_list: D002118:Calcium