title: Inhibitory effects of different substituted transition metal-based krebs-type sandwich structures on human hepatocellular carcinoma cells.
abstract: Two new transition metal-substituted Krebs-type sandwich structures of (H2imi)2[(BiW9O33)2{W0.5Ni0.5(H2O)}2{Na4(H2O)14}{Ni(H2O)3}2]·17.5H2O (3) and (H2imi)2[(BiW9O33)2(WO(OH))2Na4(H2O)13(Zn(H2O)3)2]·12H2O (4) (imi = iminazole) were synthesized using the aqueous solution method, and their structures were identified using elemental analysis, infrared, and ultraviolet spectoscopy, thermogravimetry, and single crystal X-ray diffraction. Two polyoxometalates (POMs) were isomorphic with manganese (Mn2+) and cobalt (Co2+) compounds, (Himi)2[(BiW9O33)2(W(OH)2)2(Mn(H2O)3)2(Na3(H2O)14)]·16H2O (1) and (H2imi)2[(BiW9O33)2(W(OH)2)2(Co(H2O)3)2Na4(H2O)14]·17H2O (2), which were reported in a previous study. This study continued with the determination of the inhibitory effects of four POMs on human hepatic carcinoma Hep G2 cells. Four compounds were used to compare their effects on the measurement of the sub-G1 (sub-G1 hypodiploid cell population) DNA content, the analysis of nuclear morphology, and the protein expression. The results showed that compound 3 (IC50= 25.6 ± 1.07 μmol L-1) had a much higher anti-tumor activity than compounds 1 and 4 in Hep G2 cells. Compound 2 had the lowest anti-tumor activities in Hep G2 cells. However, compound 3 showed much higher cytotoxicity than compounds 1, 2 and 4 in QSG-7701 cells. Compound 4 had the lowest cytotoxicity of all of these compounds. These findings indicate the effective relationship between the metal components and structures, and their antitumor activities in Hep G2 cells.
authors.affiliation: Department of Biochemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150090, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Synthesis of Functional Materials and Green Catalysis, Colleges of Heilongjiang Province, School of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, Heilongjiang 150025, China. zhou_bai_bin@163.com.
authors.forename: K
authors.initials: K
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Synthesis of Functional Materials and Green Catalysis, Colleges of Heilongjiang Province, School of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, Heilongjiang 150025, China. zhou_bai_bin@163.com.
authors.forename: B B
authors.identifier: http://orcid.org/0000-0001-8304-5092
authors.initials: BB
authors.lastname: Zhou
authors.affiliation: Department of Clinical Diagnosis, the 4th Affiliated Hospital, 37 YiYuan Street, Harbin 15001, China. JiaRL@ems.hrbmu.edu.cn.
authors.forename: J R
authors.initials: JR
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Cluster Science, School of Chemistry, Beijing Institute of Technology, Beijing 100081, China. ygy@bit.edu.cn.
authors.forename: G Y
authors.initials: GY
authors.lastname: Yang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D004249:DNA Damage; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000473:pathology*; D008958:Models, Molecular; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D028561:Transition Elements / Q000737:chemistry*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D009942:Organometallic Compounds; D028561:Transition Elements; D016159:Tumor Suppressor Protein p53; D014867:Water