title: Identification of host factors potentially involved in RTM-mediated resistance during potyvirus long distance movement.
abstract: The long distance movement of potyviruses is a poorly understood step of the viral cycle. Only factors inhibiting this process, referred to as "Restricted TEV Movement" (RTM), have been identified in Arabidopsis thaliana. On the virus side, the potyvirus coat protein (CP) displays determinants required for long-distance movement and for RTM-based resistance breaking. However, the potyvirus CP was previously shown not to interact with the RTM proteins. We undertook the identification of Arabidopsis factors which directly interact with either the RTM proteins or the CP of lettuce mosaic virus (LMV). An Arabidopsis cDNA library generated from companion cells was screened with LMV CP and RTM proteins using the yeast two-hybrid system. Fourteen interacting proteins were identified. Two of them were shown to interact with CP and the RTM proteins suggesting that a multiprotein complex could be formed between the RTM proteins and virions or viral ribonucleoprotein complexes. Co-localization experiments in Nicotiana benthamiana showed that most of the viral and cellular protein pairs co-localized at the periphery of chloroplasts which suggests a putative role for plastids in this process.
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Sofer
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France.|Institut National de la Recherche Scientifique-Institut Armand-Frappier, Laval, QC, H7V 1B7, Canada.
authors.forename: Daniel Garcia
authors.initials: DG
authors.lastname: Cabanillas
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gayral
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France.
authors.forename: Rachèle
authors.initials: R
authors.lastname: Téplier
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France.|Department of Botany and Plant Sciences, University of California, Riverside, CA, 92521, USA.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pouzoulet
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France.|UMR 0385 BGPI, Virus Insecte Plante, INRA, Campus international de Bailllarguet, Montpellier, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Ducousso
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France.
authors.forename: Laurène
authors.initials: L
authors.lastname: Dufin
authors.affiliation: UMR 5200, Laboratory of Membrane Biogenesis, CNRS, University of Bordeaux, 33140, Villenave d'Ornon, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Bréhélin
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Laboratoire propre du CNRS conventionné avec l'Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Ziegler-Graff
authors.affiliation: SVQV, INRA, 28 rue de Herrlisheim, 68021, Colmar, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Brault
authors.affiliation: BFP, INRA, University of Bordeaux, 33140, Villenave d'Ornon, France. frederic.revers@inra.fr.|BIOGECO, INRA, University of Bordeaux, 33615, Pessac, France. frederic.revers@inra.fr.
authors.forename: Frédéric
authors.identifier: http://orcid.org/0000-0002-6167-192X
authors.initials: F
authors.lastname: Revers
mesh_terms: D017360:Arabidopsis / Q000821:virology*; D036022:Capsid Proteins / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D018613:Microscopy, Confocal; D052585:Phloem / Q000378:metabolism / Q000821:virology; D010935:Plant Diseases / Q000821:virology; D019441:Plant Epidermis / Q000166:cytology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017800:Potyvirus / Q000502:physiology*; D021381:Protein Transport; D014026:Nicotiana / Q000502:physiology / Q000821:virology; D020798:Two-Hybrid System Techniques
chemical_list: D036022:Capsid Proteins; D010940:Plant Proteins