title: Ultrasmall Pt Nanoclusters as Robust Peroxidase Mimics for Colorimetric Detection of Glucose in Human Serum.
abstract: In this work, a new type of ultrasmall Pt nanoclusters (Pt NCs) was prepared via a facile one-pot approach by using yeast extract as the reductant and stabilizer. Besides their excellent water solubility, these yeast extract-stabilized Pt NCs also possess attractive peroxidase mimicking property. They can efficiently catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the coexistence of hydrogen peroxide (H2O2). Catalytic mechanism analysis suggested that the peroxidase mimicking activity of these Pt NCs might originate from their characteristic of accelerating electron transfer between TMB and H2O2, and their enzymatic kinetics followed typical Michaelis-Menten theory. On the basis of these findings, we developed a new highly sensitive colorimetric method for glucose detection, and the limit of detection was calculated as low as 0.28 μM (S/N = 3). Further application of the present system for glucose detection in human serum has been successfully demonstrated, suggesting its promising utilization as robust peroxidase mimics in the clinical diagnosis, pharmaceutical, and environmental chemistry fields.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry & Materials Science, Northwest University , Xi'an 710069, China.
authors.forename: Lihua
authors.identifier: http://orcid.org/0000-0002-7553-6649
authors.initials: L
authors.lastname: Jin
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry & Materials Science, Northwest University , Xi'an 710069, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Respiratory Hospital, Shaanxi Province People's Hospital , Xi'an 710068, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry & Materials Science, Northwest University , Xi'an 710069, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Cai
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry & Materials Science, Northwest University , Xi'an 710069, China.
authors.forename: Zaihua
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry & Materials Science, Northwest University , Xi'an 710069, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.affiliation: Center for Nano Energy Materials, School of Materials Science and Engineering, Northwestern Polytechnical University , Xi'an 710072, China.
authors.forename: Li
authors.identifier: http://orcid.org/0000-0003-1575-1934
authors.initials: L
authors.lastname: Shang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry & Materials Science, Northwest University , Xi'an 710069, China.
authors.forename: Yehua
authors.initials: Y
authors.lastname: Shen
mesh_terms: D040761:Biomimetic Materials; D003124:Colorimetry; D005947:Glucose; D006801:Humans; D006861:Hydrogen Peroxide; D010544:Peroxidases / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide; D010544:Peroxidases; D005947:Glucose
keywords: H2O2; Pt nanoclusters; biosensor; glucose; peroxidase mimics