title: 3D Time-lapse Imaging and Quantification of Mitochondrial Dynamics.
abstract: We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopolymer coating contains multiple functional groups which impart better cellular uptake and mitochondria targeting efficiency. The high stability of the gold nanoparticles allows continuous imaging over an extended time up to 3000 seconds without significant cell damage. By combining temporal autocorrelation analysis with a classical diffusion model, we quantify mitochondrial dynamics and cast these results into 3D maps showing the heterogeneity of diffusion parameters across the whole cell volume.
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Miguel
authors.initials: M
authors.lastname: Sison
authors.affiliation: Department of Organic Chemistry III/Macromolecular Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.|Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Sabyasachi
authors.initials: S
authors.lastname: Chakrabortty
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland.|Hepia, University of Applied Sciences of Western Switzerland (HES-SO), 4 rue de la Prairie, CH-1202 Genéve, Switzerland.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Extermann
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Amir
authors.initials: A
authors.lastname: Nahas
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Paul
authors.initials: P
authors.lastname: James Marchand
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lopez
authors.affiliation: Department of Organic Chemistry III/Macromolecular Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.|Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Weil
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser
mesh_terms: D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D063154:Mitochondrial Dynamics; D013194:Staining and Labeling / Q000379:methods; D059008:Time-Lapse Imaging / Q000379:methods*