title: An inhibitor peptide of toll-like receptor 2 shows therapeutic potential for allergic conjunctivitis.
abstract: Allergic conjunctivitis (AC) is an inflammatory disease of the conjunctiva, which is characterized by antigen challenge and toll-like receptor 2 (TLR2) activation. Here, a designed small peptide ZY12 was found to contain therapeutic potential in staphylococcal enterotoxin B (SEB)-induced AC model. ZY12 treatment showed the remission of clinical signs, plasma total IgE levels, number of mast cells and the proportion of degranulated mast cell in AC mice. Levels of Th2 cytokines (IL-4, IL-5, IL-13) in the lymph nodes or spleen were significantly decreased while those of Th1 cytokine (IFN-γ) were increased in ZY12 treated group, suggesting a protective role of ZY12 in AC by mediating the balance of Th1/Th2 cytokines. Importantly, ZY12 significantly inhibited TLR2 expression in conjunctival tissue. Combined its therapeutic effects with TLR2 inhibitory function, ZY12 might be an ideal candidate for the development of new therapeutic agent for allergic disease.
authors.affiliation: Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China; University of Chinese Academy of Sciences, Beijing 100009, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China.
authors.forename: Weihui
authors.initials: W
authors.lastname: Liu
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China; University of Chinese Academy of Sciences, Beijing 100009, China.
authors.forename: Zilei
authors.initials: Z
authors.lastname: Duan
authors.affiliation: Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China; Sino-African Joint Research Center, Chinese Academy of Science, Wuhan, Hubei 430074, China.
authors.forename: Qiumin
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China. Electronic address: yanxw@njau.edu.cn.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Yan
authors.affiliation: Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China; Sino-African Joint Research Center, Chinese Academy of Science, Wuhan, Hubei 430074, China. Electronic address: zhangzhiye5225@163.com.
authors.forename: Zhiye
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Intensive Care Unit, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, China. Electronic address: 13952721411@163.com.
authors.forename: Ruiqiang
authors.initials: R
authors.lastname: Zheng
mesh_terms: D000818:Animals; D054804:Cathelicidins / Q000737:chemistry / Q000627:therapeutic use*; D015550:Cell Degranulation / Q000187:drug effects; D003228:Conjunctiva / Q000187:drug effects* / Q000276:immunology; D003233:Conjunctivitis, Allergic / Q000188:drug therapy* / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007167:Immunotherapy / Q000379:methods*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000737:chemistry / Q000627:therapeutic use*; D012074:Remission Induction; D018417:Th1 Cells / Q000276:immunology; D058408:Th1-Th2 Balance / Q000187:drug effects*; D018418:Th2 Cells / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D054804:Cathelicidins; D016207:Cytokines; D004768:Enterotoxins; D010446:Peptide Fragments; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D007073:Immunoglobulin E; C031818:enterotoxin B, staphylococcal
keywords: Allergy conjunctivitis; Immunoregulatory peptide; Staphylococcal enterotoxin B; Toll like receptor 2