title: Development of Colloidal Gold-Based Lateral Flow Immunoassay for Rapid Qualitative and SemiQuantitative Analysis of Ustiloxins A and B in Rice Samples.
abstract: Rice false smut is a worldwide devastating rice disease infected by the fungal pathogen Villosiclava virens. Ustiloxin A (UA) and ustiloxin B (UB), cyclopeptide mycotoxins, were the major ustiloxins isolated from the rice false smut balls (FSBs) that formed in the pathogen-infected rice spikelets. Based on the specific monoclonal antibodies (mAbs) 2D3G5 and 1B5A10, respectively, against UA and UB, the lateral flow immunoassays (LFIAs) were developed, and the indicator ranges for UA and UB both were 50-100 ng/mL. The cross-reactivities of UB for UA LFIA, and UA for UB LFIA were 5% and 20%, respectively, which were consistent with the icELISA results reported previously. Even at 50,000 ng/mL, none of other commonly existent metabolites in rice samples caused noticeable inhibition. The LFIAs were used for determination of UA and UB contents in rice FSBs and rice grains, and the results were agreeable with those by HPLC and icELISA. There was no change in the sensitivity of either dipstick stored at 4 °C) after at least three months. The developed LFIA has specificity and sensitivity for detecting UA and UB as well as simplicity to use. It will be a potential point-of-care device for rapid evaluation of the rice samples contaminated by UA and UB.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. xioxiaofu@cau.edu.cn.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. erinxrs@163.com.
authors.forename: Rushan
authors.initials: R
authors.lastname: Xie
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. wangjiancau@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Crop Physiology and Cultivation, College of Agronomy and Biotechnology, Beijing 100193, China. chenxj@126.com.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. wangxiaohan99@126.com.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. sunweibo.1001@163.com.
authors.forename: Weibo
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. mengjiajiax@163.com.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Meng
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. dwlai@cau.edu.cn.
authors.forename: Daowan
authors.initials: D
authors.lastname: Lai
authors.affiliation: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. lgzhou@cau.edu.cn.
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Crop Physiology and Cultivation, College of Agronomy and Biotechnology, Beijing 100193, China. wbaomin@263.net.
authors.forename: Baomin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology; D012275:Oryza / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D009183:Mycotoxins; D010456:Peptides, Cyclic; C089077:ustiloxin B; C087237:ustiloxin A
keywords: ustiloxins A and B;   Villosiclava virens;  lateral flow immunoassay;  mycotoxin;  rice false smut balls;  rice false smut disease;  rice grains