title: [Experimental observation on the histopathological and ultrastructural pathology of Great Gerbils (Rhombomys opimus) in the Junggar Basin by subcutaneous injecting ofYersinia pestis].
abstract: Objective:To understand the histopathological and ultrastructural pathology changes of great gerbils in the Junggar Basin toYersinia pestisinfection.Methods:Forty captured great gerbils from the Junggar Basin that tested negative for anti-F1 antibodies were infected. TheY. pestisstrain 2504, isolated from a live great gerbil in the natural plague foci of the Junggar Basin in 2005 with a median lethal dose (LD(50)) of <10 CFU/ml, was used in this study. Forty great gerbils were divided into seven infection groups and were subcutaneously infected with 7.4×10(5), 7.4×10(6), 7.4×10(7), 7.4×10(8), 7.4×10(9), 7.4×10(10), or 3.0×10(11) CFU/ml of 2504. One milliliter of physiological saline was injected in the noninfected group as a control. We collected the liver, spleen, heart, and lung from all animals for histopathologic and ultrastructural pathology examination.Results:Great gerbils in the 7.4×10(8)-3.0×10(11) CFU/ml groups did not survive and exhibited pathological changes and altered ultrastructural pathology. The liver tissue of infected great gerbils showed spotty necrosis and fatty degeneration, intranuclear canaliculi with increased hepatocytes, and uneven distribution of organelles. Additionally, reactive proliferation of lymphoid tissue in the spleen, blood sinusoid lacunae with neutrophil infiltration, and phagocytosed bacteria in phagocyte cells were observed. Myocardial fiber hypertrophy and interstitial indistinction, nuclear matrices decreased in cardiac myocytes, and loose arrangement of myogenic fibers in myocardial cells were also observed. Angiectasia, capillary congestion, and tissue necrosis were found in the lung. No significant difference in histopathological and ultrastructural pathology in the parenchymal organ was observed between the 7.4×10(5)-7.4×10(7) CFU/ml groups and the 7.4×10(8)-3.0×10(11) CFU/ml groups, and no specific death caused byY. pestisinfection was apparent in the 7.4×10(5)-7.4×10(7) CFU/ml groups.Conclusion:Y. pestisinfection altered tissue and ultrastructural pathology in the parenchyma apparatus of great gerbils. In particular, the liver and spleen appeared to be the primary site ofY. pestisinfection in great gerbils.
authors.affiliation: Xinjiang Uygur Autonomous Regional Center for disease Control and Prevention, Urumqi 830002, Xinjiang, China.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Rehemu
authors.initials: R
authors.lastname: Azhati
authors.forename: W W
authors.initials: WW
authors.lastname: Meng
authors.forename: T
authors.initials: T
authors.lastname: Luo
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Maituohuti
authors.initials: M
authors.lastname: Abulimiti
authors.forename: X H
authors.initials: XH
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Dai
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D002681:China / Q000453:epidemiology; D005849:Gerbillinae / Q000276:immunology* / Q000382:microbiology; D008099:Liver / Q000276:immunology / Q000382:microbiology; D051379:Mice; D010930:Plague / Q000097:blood / Q000453:epidemiology* / Q000276:immunology* / Q000382:microbiology; D012376:Rodent Diseases / Q000453:epidemiology* / Q000382:microbiology; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D015010:Yersinia pestis / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology
chemical_list: D000907:Antibodies, Bacterial
keywords: Pathology; Rats; Yersinia pestis