title: Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface.
abstract: Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to determine suitable concentrations of RB in these bonding agents for imaging in the confocal laser scanning microscope. This study provides systematical strategies for adding RB to viscous dental adhesive resins, focusing on the determination of the lowest range of dye concentrations necessary to achieve an acceptable image of the dentin/adhesive interface. It was demonstrated that optimized images of the resin distribution in dentin can be produced with 0.1-0.02 mg/mL of RB in the (tested) adhesives. Our approaches took into account aspects related to the dye concentration, photophysical parameters in different host media, specimen composition and morphology to develop a rational use of the fluorescent agent with the resin-based materials. Information gained from this work can help optimize labeling methods using dispersions of low-molecular-weight dyes in different monomer blend systems.
authors.affiliation: 1Department of Operative Dentistry,Endodontics and Dental Materials,Bauru School of Dentistry,University of São Paulo,Bauru,17012-901 SP,Brazil.
authors.forename: Odair
authors.initials: O
authors.lastname: Bim Júnior
authors.affiliation: 2Department of Inorganic Chemistry,Institute of Chemistry,Universidade Estadual Paulista,Araraquara,14800-060 SP,Brazil.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Cebim
authors.affiliation: 1Department of Operative Dentistry,Endodontics and Dental Materials,Bauru School of Dentistry,University of São Paulo,Bauru,17012-901 SP,Brazil.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Atta
authors.affiliation: 3Department of Prosthodontics,Bauru School of Dentistry, University of São Paulo, Bauru,17012-901 SP,Brazil.
authors.forename: Camila M
authors.initials: CM
authors.lastname: Machado
authors.affiliation: 4Department of Operative Dentistry,School of Dentistry, University of São Paulo,São Paulo, 05508-000 SP,Brazil.
authors.forename: Luciana F
authors.initials: LF
authors.lastname: Francisconi-Dos-Rios
authors.affiliation: 1Department of Operative Dentistry,Endodontics and Dental Materials,Bauru School of Dentistry,University of São Paulo,Bauru,17012-901 SP,Brazil.
authors.forename: Linda
authors.initials: L
authors.lastname: Wang
mesh_terms: D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003764:Dental Materials / Q000737:chemistry; D003793:Dental Restoration, Permanent; D003804:Dentin / Q000000981:diagnostic imaging; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D018613:Microscopy, Confocal; D019279:Resin Cements / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D012235:Rhodamines; D013194:Staining and Labeling / Q000379:methods*; D013499:Surface Properties
chemical_list: C438313:Clearfil SE Bond; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; D019279:Resin Cements; D012117:Resins, Synthetic; D012235:Rhodamines; C041330:Scotchbond; C029773:rhodamine B
keywords: confocal laser microscopy; dental adhesives; dentin bonding; fluorescent dyes; interfacial morphology; photoluminescence spectroscopy