title: Trehalose improves rabbit sperm quality during cryopreservation.
abstract: High levels of reactive oxygen species are associated with spermatozoa cryopreservation, which bring damage to functional spermatozoa. The aim of the present study was to investigate whether and how the freezing extenders supplemented with trehalose was beneficial for the survival of rabbit spermatozoa. semen was diluted with Tris-citrate-glucose extender addition of different concentrations of trehalose. Addition of 100 mM trehaose significantly improved post-thaw rabbit sperm parameters, such as motility, acrosome integriy, membrane integrity and mitochondrial membrane potential. Moreover, when freezing extenders supplemented with trehalose, activities of catalase (CAT), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) of post-thaw spermatozoa were enhanced, meanwhile, reactive oxygen species (ROS) level and Malondialdehyde (MDA) content were decreased. The results suggest that freezing extenders supplemented with 100 mM trehalose resulted in less ROS level and MDA content, higher motility and mitochondrial membrane potential as well as the integrity of acrosome and plasma membrane. Supplementation of trehalose with freezing extenders is beneficial to the rabbit breeding industry.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China.
authors.forename: Xiaoteng
authors.initials: X
authors.lastname: Fan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China. Electronic address: zengwenxian2013@126.com.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Zeng
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D002374:Catalase / Q000378:metabolism; D019343:Citric Acid / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D005947:Glucose / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014199:Trehalose / Q000494:pharmacology*; D014325:Tromethamine / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D017382:Reactive Oxygen Species; D014325:Tromethamine; D019343:Citric Acid; D008315:Malondialdehyde; D014199:Trehalose; D002374:Catalase; D013482:Superoxide Dismutase; D005947:Glucose
keywords: Antioxidants; Cryopreservation; Rabbit; Semen; Trehalose