title: Noncoding Effects of Circular RNA CCDC66 Promote Colon Cancer Growth and Metastasis.
abstract: Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly unknown. Recent studies indicate circRNA may be involved in disease pathogenesis, but direct evidence is scarce. Here, we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-of-function studies in colorectal cancer cell lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis.Cancer Res; 77(9); 2339-50. ©2017 AACR.
authors.affiliation: Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Kuei-Yang
authors.initials: KY
authors.lastname: Hsiao
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ya-Chi
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas.
authors.forename: Sachin Kumar
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ning
authors.initials: N
authors.lastname: Chang
authors.affiliation: Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas.
authors.forename: Laising
authors.initials: L
authors.lastname: Yen
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. seantsai@mail.ncku.edu.tw hssun@mail.ncku.edu.tw.
authors.forename: H Sunny
authors.initials: HS
authors.lastname: Sun
authors.affiliation: Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. seantsai@mail.ncku.edu.tw hssun@mail.ncku.edu.tw.
authors.forename: Shaw-Jenq
authors.initials: SJ
authors.lastname: Tsai
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D051379:Mice; D009362:Neoplasm Metastasis; D011127:Polyps / Q000235:genetics* / Q000473:pathology; D012313:RNA / Q000096:biosynthesis / Q000235:genetics*; D000079962:RNA, Circular; D022661:RNA, Untranslated / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C000618179:CCDC66 protein, human; D005136:Eye Proteins; D000079962:RNA, Circular; D022661:RNA, Untranslated; D012313:RNA