title: Immunohistochemical Expression of the Tumor Suppressor Protein p16INK4a in Cervical Adenocarcinoma.
abstract: ObjectiveTo evaluate the diagnostic utility of the p16ink4aprotein expression as a marker for adenocarcinoma of the cervix.MethodsIn a cross-sectional study, p16ink4aexpression was evaluated in 30 cervical biopsies from patients diagnosed with invasive adenocarcinoma from 2 reference clinics in Brazil, and compared with 18 biopsies of endocervical polyps (control cases). The performance of the tests for p16ink4awas evaluated using a conventional contingency table, and the Kappa (κ) index was used to evaluate the agreement of the marker with the tissue diagnosis.ResultsIn total, 66% of the invasive adenocarcinoma cases were positive for p16ink4a. All of the adenomatous polyps cases used as negative controls were shown to be negative for p16ink4a. The marker showed a high sensitivity and a high negative predictive value. The Kappa index was good for p16ink4a(κ = 0.6).ConclusionConsidering the strong association between the p16ink4amarker and the cervical adenocarcinoma, its use represents an important tool for reducing incorrect diagnoses of adenocarcinoma and thereby avoiding overtreatment.
authors.affiliation: Department of Pathology, Faculty of Medicine, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil.
authors.forename: José
authors.initials: J
authors.lastname: Eleutério
authors.affiliation: Department of Pathology, Faculty of Medicine, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil.
authors.forename: Thiago Silva
authors.initials: TS
authors.lastname: Lima
authors.affiliation: Department of Surgical Pathology, Instituto do Câncer do Ceará, Fortaleza, CE, Brazil.
authors.forename: Maria do Perpétuo Socorro
authors.initials: MD
authors.lastname: Cunha
authors.affiliation: Department of Pathology, Faculty of Medicine, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil.
authors.forename: Diane Isabelle Magno
authors.initials: DI
authors.lastname: Cavalcante
authors.affiliation: Department of Pathology, Faculty of Medicine, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil.
authors.forename: Angélica Maria Holanda
authors.initials: AM
authors.lastname: Silva
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D003430:Cross-Sectional Studies; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16