title: Volumetric Bar-Chart Chips for Biosensing.
abstract: The volumetric bar-chart chip (V-Chip) is a microfluidics-based, point-of-care (POC) device for the multiplexed and quantitative measurement of biomarkers. Volumetric readouts, based on the measurement of oxygen generated by a reaction between catalase and hydrogen peroxide, allow instant visual quantitation of target biomarkers and provide visualized bar charts without any assistance from instruments and without the need for data processing or graphics plotting. V-Chip shows potential capabilities in POC and personalized diagnostics; for instance, it can be utilized for making high-throughput, multiplexed, and quantitative measurements. Further, this system is highly portable and can be performed at low cost. The development of the V-Chip thus marks a POC milestone and opens up the possibility of instrument-free personalized diagnostics. Here, we describe the protocols for the fabrication of V-Chip and the use of silica nanoparticles as the probe carrier for the V-Chip-based enzyme-linked immunosorbent assay (ELISA) for the detection of biomarkers.
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA.|Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY, 10065, USA.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA.|Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY, 10065, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA. lqin@HoustonMethodist.org.|Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY, 10065, USA. lqin@HoustonMethodist.org.
authors.forename: Lidong
authors.initials: L
authors.lastname: Qin
mesh_terms: D015415:Biomarkers; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000737:chemistry; D002374:Catalase / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D018796:Immunoconjugates; D056656:Lab-On-A-Chip Devices; D019095:Point-of-Care Systems
chemical_list: D015415:Biomarkers; D002272:Carcinoembryonic Antigen; D018796:Immunoconjugates; D002374:Catalase
keywords: Biomarkers; ELISA; Multiplex; Point-of-care; Quantitation