title: Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering.
abstract: Neural tissue engineering and three-dimensional in vitro tissue modeling require the development of biomaterials that take into account the specified requirements of human neural cells and tissue. In this study, an alternative method of producing biomimetic hydrogels based on gellan gum (GG) was developed by replacing traditional crosslinking methods with the bioamines spermidine and spermine. These bioamines were proven to function as crosslinkers for GG hydrogel at +37 °C, allowing for the encapsulation of human neurons. We studied the mechanical and rheological properties of the formed hydrogels, which showed biomimicking properties comparable to naïve rabbit brain tissue under physiologically relevant stress and strain. Human pluripotent stem cell-derived neuronal cells demonstrated good cytocompatibility in the GG-based hydrogels. Moreover, functionalization of GG hydrogels with laminin resulted in cell type-specific behavior: neuronal cell maturation and neurite migration.
authors.affiliation: BioMediTech Institute and Faculty of Biomedical Sciences and Engineering, Tampere University of Technology, Korkeakoulunkatu 3, FI-33720 Tampere, Finland. BioMediTech Institute and Faculty of Medicine and Life Sciences, University of Tampere, Lääkärinkatu 1, FI-33520 Tampere, Finland.
authors.forename: Janne T
authors.initials: JT
authors.lastname: Koivisto
authors.forename: Tiina
authors.initials: T
authors.lastname: Joki
authors.forename: Jenny E
authors.initials: JE
authors.lastname: Parraga
authors.forename: Rami
authors.initials: R
authors.lastname: Pääkkönen
authors.forename: Laura
authors.initials: L
authors.lastname: Ylä-Outinen
authors.forename: Laura
authors.initials: L
authors.lastname: Salonen
authors.forename: Ilari
authors.initials: I
authors.lastname: Jönkkäri
authors.forename: Marja
authors.initials: M
authors.lastname: Peltola
authors.forename: Teemu O
authors.initials: TO
authors.lastname: Ihalainen
authors.forename: Susanna
authors.initials: S
authors.lastname: Narkilahti
authors.forename: Minna
authors.initials: M
authors.lastname: Kellomäki
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D040761:Biomimetic Materials / Q000737:chemistry; D002454:Cell Differentiation; D003432:Cross-Linking Reagents; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology; D007797:Laminin / Q000502:physiology; D008297:Male; D008422:Materials Testing; D009417:Nerve Tissue / Q000166:cytology / Q000502:physiology*; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011817:Rabbits; D012212:Rheology; D013095:Spermidine; D013096:Spermine; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D007797:Laminin; D011135:Polysaccharides, Bacterial; D013096:Spermine; C048288:gellan gum; D013095:Spermidine