title: Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen.
abstract: We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.
authors.affiliation: School of Chemistry, The University of Melbourne, Parkville VIC 3010, Australia. trevoras@unimelb.edu.au.
authors.forename: Hamid
authors.initials: H
authors.lastname: Soleimaninejad
authors.affiliation: School of Chemistry, The University of Melbourne, Parkville VIC 3010, Australia. trevoras@unimelb.edu.au.
authors.forename: Moore Z
authors.initials: MZ
authors.lastname: Chen
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, P. R. China.
authors.forename: Xiaoding
authors.initials: X
authors.lastname: Lou
authors.affiliation: School of Chemistry, The University of Melbourne, Parkville VIC 3010, Australia. trevoras@unimelb.edu.au.
authors.forename: Trevor A
authors.initials: TA
authors.lastname: Smith
authors.affiliation: School of Chemistry, The University of Melbourne, Parkville VIC 3010, Australia. trevoras@unimelb.edu.au and Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia. Y.Hong@latrobe.edu.au.
authors.forename: Yuning
authors.identifier: http://orcid.org/0000-0002-8085-1651
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure
chemical_list: D000588:Amines; D005456:Fluorescent Dyes; D046911:Macromolecular Substances