title: Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by EngineeredStreptomyces avermitilis.
abstract: Heavy-ion irradiation technology has advantages over traditional methods of mutagenesis. Heavy-ion irradiation improves the mutation rate, broadens the mutation spectrum, and shortens the breeding cycle. However, few data are currently available regarding its effect onStreptomyces avermitilismorphology and productivity. In this study, the influence of heavy-ion irradiation onS. avermitiliswhen cultivated in approximately 10 L stirred-tank bioreactors was investigated. The specific productivity of the avermectin (AVM) B1a-producing mutantS. avermitilis147-G58 increased notably, from 3885 to 5446μg/mL, approximately 1.6-fold, compared to the original strain. The mycelial morphology of the mutant fermentation processes was microscopically examined. Additionally, protein and metabolite identification was performed by using SDS-PAGE, 2- and 3-dimensional electrophoresis (2DE and 3DE). The results showed that negative regulation gene deletion of mutants led to metabolic process upregulating expression of protein and improving the productivity of an avermectin B1a. The results showed that the heavy-ion beam irradiation dose that corresponded to optimal production was well over the standard dose, at approximately 80 Gy at 220 AMeV (depending on the strain). This study provides reliable data and a feasible method for increasing AVM productivity in industrial processes.
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China; Lanzhou University, 222 South Tianshui Road, Lanzhou, Gansu 730000, China.
authors.forename: Shu-Yang
authors.identifier: 0000-0001-9184-7394
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Institute of Veterinary Drug Quality Inspection of Shandong Province, Jinan 250022, China.
authors.forename: Yong-Heng
authors.initials: YH
authors.lastname: Bo
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Xiang
authors.identifier: 0000-0002-7696-4290
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Liang
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Xiao
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China.
authors.forename: Bo-Ling
authors.initials: BL
authors.lastname: Jiang
mesh_terms: D019149:Bioreactors; D002244:Carbon / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005818:Genetic Engineering / Q000379:methods*; D020450:Heavy Ions; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D059645:Mutation Rate; D025282:Mycelium / Q000166:cytology / Q000528:radiation effects; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D013997:Time Factors
chemical_list: C022120:avermectin B(1)a; D007559:Ivermectin; D002244:Carbon