title: Osteogenic Differentiation of MSCs on Fibronectin-Coated and nHA-Modified Scaffolds.
abstract: The increasing demand for biocompatible bone substitutes has made it a priority to tissue engineering and regenerative medicine scientists. Combination of minerals, growth factors, and extracellular matrix (ECM) proteins with nanofibrous scaffolds is a potential promising strategy for bone reconstruction and clinical applications. In this study, nanohydroxyapatite (nHA) was incorporated in electrospun nanofibrous polycaprolactone (PCL) scaffolds coated with fibronectin (Fn). The potential bone regeneration capacities of these scaffolds were evaluated in vitro and in vivo using mouse mesenchymal stem cells (mMSCs). The interconnected pores and proper mechanical characteristics of the fabricated electrospun PCL mats in combination with nHA and Fn provided suitable environment for cell attachment, proliferation, and enhanced osteogenic differentiation. The synergistic effect of Fn and nHA on the both in vitro and in vivo increase of calcium deposition was assessed by biochemical analysis. In addition, alkaline phosphatase (ALP) activity in nHA-incorporated PCL scaffold (PCL/nHA) and Fn-coated PCL/nHA (PCL/nHA/Fn) were significantly higher in comparison to the control group. Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) analyses of important bone-related genes (ALP, osteocalcin, osteopontin, and Runx2) revealed that Fn has additive effect on promoting the osteogenic differentiation. The aforementioned results indicated that nanofibrous PCL/nHA scaffold coated with Fn is a promising candidate for bone-tissue engineering applications.
authors.affiliation: From the *Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran; †Stem Cell Technology Research Center, Tehran, Iran; ‡Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; and §Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical sciences, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Mohamadyar-Toupkanlou
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Vasheghani-Farahani
authors.forename: Hana
authors.initials: H
authors.lastname: Hanaee-Ahvaz
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Masumeh
authors.initials: M
authors.lastname: Dodel
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Havasi
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.forename: Elham Sadat
authors.initials: ES
authors.lastname: Taherzadeh
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D010012:Osteogenesis; D011091:Polyesters / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D005353:Fibronectins; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite