title: Coupling genetic code expansion and metabolic engineering for synthetic cells.
abstract: Orthogonal protein translation with noncanonical amino acids (ncAAs) has become a standard method in biosciences. Whereas much effort is made to broaden the chemical space of ncAAs, only few attempts on their systematic low-cost in situ production are reported until now. The main aim is to engineer cells with newly designed biosynthetic pathways coupled with orthogonal aminoacyl-tRNA synthetase/tRNA pairs (o-pairs). These should provide cost-effective solutions to industrially relevant bio-production problems, such as peptide/protein production beyond the canonical set of natural molecules and to expand the arsenal of chemistries available for living cells. Therefore, coupling genetic code expansion (GCE) with metabolic engineering is the basic prerequisite to transform orthogonal translation from a standard technique in academic research to industrial biotechnology.
authors.affiliation: Department of Chemistry, Technische Universität Berlin, Müller-Breslau-Straße 10, 10623 Berlin, Germany.
authors.forename: Jan-Stefan
authors.initials: JS
authors.lastname: Völler
authors.affiliation: Department of Chemistry, Technische Universität Berlin, Müller-Breslau-Straße 10, 10623 Berlin, Germany. Electronic address: nediljko.budisa@tu-berlin.de.
authors.forename: Nediljko
authors.initials: N
authors.lastname: Budisa
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics; D000818:Animals; D058045:Artificial Cells / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods / Q000639:trends; D005815:Genetic Code / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D060847:Metabolic Engineering / Q000379:methods*; D010455:Peptides / Q000235:genetics; D011506:Proteins / Q000235:genetics
chemical_list: D000596:Amino Acids; D010455:Peptides; D011506:Proteins; D000604:Amino Acyl-tRNA Synthetases