title: Simultaneous determination of wogonin, oroxylin a, schisandrin, paeoniflorin and emodin in rat serum by HPLC-MS/MS and application to pharmacokinetic studies.
abstract: Wogonin and oroxylin A in Scutellariae Radix, schisandrin in Chinensis Fructus, paeoniflorin in Moutan Cortex and emodin in Polygoni Cuspidate Rhizome et Radix are anti-inflammatory active compounds. A method for simultaneous determination of the five compounds in rat was developed and validated using high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The separation was performed on a Symmetry C18column (4.6 × 50 mm, 3.5 μm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phases. The detection was performed using multiple-reaction monitoring with electrospray ionization source in positive-negative ion mode. The calibration curves showed good linearity (r ≥ 0.9955). The lower limit of quantification (LLOQ) was 5 ng/mL for wogonin and schisandrin, 10 ng/mL for oroxylin A and emodin, and 15 ng/mL for paeoniflorin, respectively. The relative standard deviations of intraday and interday precisions were <11.49 and 14.28%, respectively. The extraction recoveries and matrix effects were acceptable. The analytes were stable under the experiment conditions. The validated method has been successfully applied to pharmacokinetic studies of the five compounds in rats after oral administration of Hu-gan-kan-kang-yuan capsule. This paper would be a valuable reference for pharmacokinetic studies of Chinese medicine preparations containing the five compounds.
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Minting
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Chemistry and Biochemistry, Lamar University, Beaumont, Texas, USA.
authors.forename: Suying
authors.initials: S
authors.lastname: Wei
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Chaohua
authors.initials: C
authors.lastname: Luo
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Feilong
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Song
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Shen
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Mo
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Fenghuan
authors.identifier: http://orcid.org/0000-0003-3135-2394
authors.initials: F
authors.lastname: Wei
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034242:Cyclooctanes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal; D004642:Emodin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D044950:Flavanones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D017705:Lignans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D039821:Monoterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D011083:Polycyclic Compounds / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D006027:Glycosides
chemical_list: D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal; D004642:Emodin; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D017705:Lignans; D039821:Monoterpenes; D011083:Polycyclic Compounds; C015423:peoniflorin; D006027:Glycosides; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; C011105:schizandrin; C085514:wogonin
keywords: HPLC-MS/MS; Hu-Gan-kan-Kang-yuan capsule; pharmacokinetics; positive-negative ion mode; simultaneous determination