title: Quantum-Mechanical Study on the Catalytic Mechanism of Alkaline Phosphatases.
abstract: Alkaline phosphatases (APs) catalyze the hydrolysis and transphosphorylation of phosphate monoesters. The catalytic mechanism was examined by quantum-mechanical calculations using an active-site model based on the X-ray crystal structure of the human placental AP. Free energies of activation and of reaction for the catalytic steps were evaluated for a series of aryl and alkyl phosphate esters, and the computational results were compared with experimental values available in the literature. Mechanistic observations previously reported in experimental works were rationalized by the present theoretical study, particularly regarding the difference in the rate-determining step between aryl and alkyl phosphates. The formation rate of the covalent phosphoserine intermediate followed a linear free energy relationship (LFER) with the pKaof the leaving group. This LFER, which could be experimentally determined only for less reactive alkyl phosphates, was verified by the present calculations to apply for the entire set of aryl and alkyl phosphate substrates.
authors.affiliation: INFIQC, CONICET and Departamento de Química Teórica y Computacional, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba , Ciudad Universitaria, Córdoba 5000, Argentina.
authors.forename: Gabriela L
authors.identifier: 0000-0001-7660-962X
authors.initials: GL
authors.lastname: Borosky
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D020134:Catalytic Domain; D006868:Hydrolysis; D008958:Models, Molecular; D011789:Quantum Theory; D013816:Thermodynamics
chemical_list: D000469:Alkaline Phosphatase