title: Multiple Pesticides Detoxification Function of Maize (Zea mays) GST34.
abstract: ZmGST34 is a maize Tau class GST gene and was found to be differently expressed between two maize cultivars differing in tolerance to herbicide metolachlor. To explore the possible role of ZmGST34 in maize development, the expression pattern and substrate specificity of ZmGST34 were characterized by quantitative RT-PCR and heterologous expression system, respectively. The results indicated that the expression level of ZmGST34 was increased ∼2-5-fold per day during the second-leaf stage of maize seedling. Chloroacetanilide herbicides or phytohormone treatments had no influence on the expression level of ZmGST34, suggesting that ZmGST34 is a constitutively expressed gene in maize seedling. Heterologous expression in Escherichia coli and in Arabidopsis thaliana proved that ZmGST34 can metabolize most chloroacetanilide herbicides and increase tolerance to these herbicides in transgenic Arabidopsis thaliana. The constitutive expression pattern and broad substrate activity of ZmGST34 suggested that this gene may play an important role in maize development in addition to the detoxification of pesticides.
authors.affiliation: College of Science, China Agricultural University , No. 2 of Yuan Ming Yuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Dongzhi
authors.identifier: http://orcid.org/0000-0002-1245-9244
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Science, China Agricultural University , No. 2 of Yuan Ming Yuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Science, China Agricultural University , No. 2 of Yuan Ming Yuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Pang
authors.affiliation: Department of Economic Development, Jobs, Transport and Resources, AgriBio, Centre for AgriBioscience, La Trobe University , 5 Ring Road, Bundoora, Victoria 3083, Australia.
authors.forename: Zhiqian
authors.identifier: http://orcid.org/0000-0001-6324-3390
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Science, China Agricultural University , No. 2 of Yuan Ming Yuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Weisong
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Science, China Agricultural University , No. 2 of Yuan Ming Yuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Chengju
authors.initials: C
authors.lastname: Wang
mesh_terms: D018506:Gene Expression Regulation, Plant; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006540:Herbicides / Q000378:metabolism*; D008658:Inactivation, Metabolic; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D006540:Herbicides; D010940:Plant Proteins; D005982:Glutathione Transferase
keywords: ZmGST34; chloroacetanilide herbicides; constitutive expression; expression pattern; heterologous expression; substrate specificity