title: HPLC-DAD-QTOF-MS profiling of phenolics from leaf extracts of two Tunisian fig cultivars: Potential as a functional food.
abstract: The phenolic constituents of the aqueous-ethanolic extract of two Tunisian Ficus carica leaves cultivars and their hypoglycaemic, hypolipidaemic and antioxidative activities in alloxan-induced diabetic rats were investigated. Our results demonstrated that the treatment with the leaves extracts of F. carica improved lipid profile and reduced blood glucose level as well as thiobarbituric acid-reactive substances content. The antioxidant enzymes activity in the liver and heart tissues of diabetic rats was increased after the treatment. These antihyperglycaemic, antihyperlipidaemic and antioxidant effects of both leaf extracts could be associated with their in vitro scavenging ability and their phenolic composition. The HPLC-DAD-QTOF-MS analysis of both extracts revealed the presence of dihydroxybenzoic acid, dipentoside, rutin, psoralen, methoxypsoralen and oxypeudacin hydrate as relatively the most abundant compounds. These results showed indicated the capacity of the leaves extracts of F. carica to ameliorate hyperglycaemia, hyperlipidaemia and antioxidant status in diabetic rats.
authors.affiliation: Laboratoire d'Ecophysiologie Animale Faculté des Sciences de Sfax, Université de Sfax, Tunisie.
authors.forename: Olfa
authors.initials: O
authors.lastname: Belguith-Hadriche
authors.affiliation: Laboratoire d'Electrochimie et Environnement, Ecole national d'ingénieur de Sfax, BP 1177, Université de Sfax, Tunisie.
authors.forename: Sonda
authors.initials: S
authors.lastname: Ammar
authors.affiliation: Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avda. del Conocimiento s/n, 18016, Granada, Spain; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.
authors.forename: María Del Mar
authors.initials: MDM
authors.lastname: Contreras
authors.affiliation: Laboratoire de Toxicologie- Microbiologie Environnementale & santé (UR/11 ES70), Université de Sfax, Tunisie.
authors.forename: Hamadi
authors.initials: H
authors.lastname: Fetoui
authors.affiliation: Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avda. del Conocimiento s/n, 18016, Granada, Spain; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura-Carretero
authors.affiliation: Laboratoire d'Ecophysiologie Animale Faculté des Sciences de Sfax, Université de Sfax, Tunisie.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.affiliation: Laboratoire d'Electrochimie et Environnement, Ecole national d'ingénieur de Sfax, BP 1177, Université de Sfax, Tunisie. Electronic address: mohamed.bouaziz@fsg.rnu.tn.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bouaziz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001786:Blood Glucose / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D030681:Ficus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D055951:Functional Food; D006943:Hyperglycemia / Q000188:drug therapy / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000494:pharmacology; D008055:Lipids / Q000097:blood; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D008055:Lipids; D010636:Phenols; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances
keywords: Antioxidant enzymes; Diabetic-rats; Ficus carica; Furanocoumarins; Mass spectrometry; Phenolic compounds