title: The effect of ovine peripheral blood mononuclear cells on Haemonchus contortus larval morbidity in vitro.
abstract: The objective of this study was to determine the effects of peripheral blood mononuclear cells (PBMC), derived from parasite-resistant St. Croix (STC) hair sheep and parasite-susceptible Suffolk (SUF) sheep, on Haemonchus contortus L3stage larval death in vitro, with or without autologous serum. Larval morbidity was quantified by measuring larval ATP concentration following incubation with PBMC. Larvae exposed to either STC- or SUF-derived PBMC had lower ATP than live larvae (0.12 μmol/L ATP and 0.16 μmol/L ATP vs 0.27 μmol/L ATP, respectively) (P<.001) and greater ATP of dead larvae (0.03 μmol/L ATP) (P<.001). Breed differences were observed with addition of autologous serum. Larvae exposed to SUF-derived PBMC with autologous serum were not significantly different from live larval ATP. STC-derived serum did not significantly reduce larval ATP compared to PBMC alone (0.11 μmol/L ATP), but was significantly reduced compared to live larvae (0.22 μmol/L ATP) and SUF-derived PBMC with autologous serum (0.23 μmol/L ATP) (P<.001). These data indicate that a cellular response alone is capable of significantly reducing larval ATP in a breed-independent manner. However, addition of serum to SUF-PBMC failed to reduce larval ATP, indicating breed-dependent humoral response to H. contortus.
authors.affiliation: Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Shepherd
authors.affiliation: Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, USA.
authors.forename: J J
authors.identifier: 0000-0003-4747-0950
authors.initials: JJ
authors.lastname: Garza
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Greiner
authors.affiliation: Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, USA.
authors.forename: S A
authors.identifier: 0000-0002-2624-8835
authors.initials: SA
authors.lastname: Bowdridge
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D060467:Disease Resistance; D006188:Haemonchiasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000254:growth & development / Q000502:physiology*; D007814:Larva / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000469:parasitology; D034561:Sheep, Domestic; D013045:Species Specificity
chemical_list: D000255:Adenosine Triphosphate
keywords: 
  ATP
  ; 
  Haemonchus contortus
  ; St. Croix; cellular Immunity; larval motility