title: Osteogenic induction of bone marrow mesenchymal cells on electrospun polycaprolactone/chitosan nanofibrous membrane.
abstract: A novel chitosan/polycaprolactone (CS/PCL) nanofibrous membrane by electrospinning was developed for guided tissue regeneration (GTR) to improve mechanical properties and to promote osteogenic differentiation. Firstly, chitosan and PCL solutions of different weight ratios (0/100, 30/70, 50/50) were mixed and then electrospun. Our data demonstrated that the CS/PCL (30/70) nanofibrous membrane promoted an increased rBMSCs proliferation when compared to the CS/PCL (50/50) membrane and pure PCL (0/100) membrane. The highest ALP activity and extracellular calcium deposit were observed on the CS/PCL (30/70) nanofibrous membrane, followed by the CS/PCL (50/50) and pure PCL nanofibrous membrane. Furthermore, the expression of osteocalcin (OCN) and Runx2 were also significantly higher on the CS/PCL (30/70, 50/50) nanofibrous membrane as compared to the pure PCL nanofibrous membrane. In conclusion, the electrospun CS/PCL nanofibrous membrane was found to be a biocompatible material that could stimulate osteogenic differentiation, suggesting that the novel CS/PCL membrane has an interesting potential as use for GTR.
authors.affiliation: Key Lab of Oral Diseases Research of Anhui Province, Stomatologic Hospital & College, Anhui Medical University.|Department of Stomatology, The Second People's Hospital of Wuhu.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: Surgical Department one, Wuhu Traditional Chinese.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Lab of Oral Diseases Research of Anhui Province, Stomatologic Hospital & College, Anhui Medical University.
authors.forename: Xuyan
authors.initials: X
authors.lastname: Tang
authors.affiliation: Shanghai Biomaterials Research & Testing Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
mesh_terms: D001853:Bone Marrow; D049109:Cell Proliferation; D048271:Chitosan; D057139:Nanofibers; D010012:Osteogenesis; D011091:Polyesters; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; D048271:Chitosan
keywords: Bone marrow mesenchymal stem cell; Chitosan; Electrospun; Osteogenic differentiation; Polycaprolactone