title: Ultrasensitive Detection of Aminopeptidase N Activity in Urine and Cells with a Ratiometric Fluorescence Probe.
abstract: An ultrasensitive ratiometric fluorescent probe (CVN) has been designed and synthesized by incorporating alanine into the cresyl violet fluorophore. The probe shows ratiometric fluorescence response toward aminopeptidase N (APN) through the increase of fluorescent intensity ratio of 626/575 nm. The sensitivity of the probe is ultrahigh with a detection limit of 33 pg/mL, which can quantify the contents of APN in 500-fold diluted human urine samples. Furthermore, by using ratiometric fluorescence imaging, the probe reveals significantly higher contents of APN in HepG2 cells than those in LO2 cells, which has been further used to distinguish these two types of cells in mixed cocultures. The probe could be of great importance for the APN-related disease diagnosis and pathophysiology elucidation.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: He
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Wen
authors.identifier: 0000-0002-2368-3997
authors.initials: W
authors.lastname: Shi
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Ma
mesh_terms: D048588:Benzoxazines / Q000138:chemical synthesis / Q000737:chemistry; D018826:CD13 Antigens / Q000032:analysis* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008856:Microscopy, Fluorescence
chemical_list: D048588:Benzoxazines; D005456:Fluorescent Dyes; C028911:cresyl violet; D018826:CD13 Antigens