title: Development of minimal enzyme cocktails for hydrolysis of sulfite-pulped lignocellulosic biomass.
abstract: Despite recent progress, saccharification of lignocellulosic biomass is still a major cost driver in biorefining. In this study, we present the development of minimal enzyme cocktails for hydrolysis of Norway spruce and sugarcane bagasse, which were pretreated using the so-called BALI™ process, which is based on sulfite pulping technology. Minimal enzyme cocktails were composed using several glycoside hydrolases purified from the industrially relevant filamentous fungus Trichoderma reesei and a purified commercial β-glucosidase from Aspergillus niger. The contribution of in-house expressed lytic polysaccharide monooxygenases (LPMOs) was also tested, since oxidative cleavage of cellulose by such LPMOs is known to be beneficial for conversion efficiency. We show that the optimized cocktails permit efficient saccharification at reasonable enzyme loadings and that the effect of the LPMOs is substrate-dependent. Using a cocktail comprising only four enzymes, glucan conversion for Norway spruce reached >80% at enzyme loadings of 8mg/g glucan, whereas almost 100% conversion was achieved at 16mg/g.
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
authors.forename: Piotr
authors.initials: P
authors.lastname: Chylenski
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
authors.forename: Zarah
authors.initials: Z
authors.lastname: Forsberg
authors.affiliation: Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Jerry
authors.initials: J
authors.lastname: Ståhlberg
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
authors.forename: Anikó
authors.initials: A
authors.lastname: Várnai
authors.affiliation: Borregaard AS, Sarpsborg, Norway.
authors.forename: Martin
authors.initials: M
authors.lastname: Lersch
authors.affiliation: Borregaard AS, Sarpsborg, Norway.
authors.forename: Oskar
authors.initials: O
authors.lastname: Bengtsson
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
authors.forename: Solve
authors.initials: S
authors.lastname: Sæbø
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.
authors.forename: Svein Jarle
authors.initials: SJ
authors.lastname: Horn
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway. Electronic address: vincent.eijsink@nmbu.no.
authors.forename: Vincent G H
authors.initials: VGH
authors.lastname: Eijsink
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D018533:Biomass; D002482:Cellulose / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D028222:Picea / Q000737:chemistry; D013379:Substrate Specificity; D013447:Sulfites / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D013447:Sulfites; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D006899:Mixed Function Oxygenases; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase
keywords: Cellulase; LPMO; Lignocellulose; Saccharification; Trichoderma reesei