title: RepSox improves viability and regulates gene expression in rhesus monkey-pig interspecies cloned embryos.
abstract: OBJECTIVE
  To investigate the effect of the small molecule, RepSox, on the expression of developmentally important genes and the pre-implantation development of rhesus monkey-pig interspecies somatic cell nuclear transfer (iSCNT) embryos.
  
  
  RESULTS
  Rhesus monkey cells expressing the monomeric red fluorescent protein 1 which have a normal (42) chromosome complement, were used as donor cells to generate iSCNT embryos. RepSox increased the expression levels of the pluripotency-related genes, Oct4 and Nanog (p < 0.05), but not of Sox2 compared with untreated embryos at the 2-4-cell stage. Expression of the anti-apoptotic gene, Bcl2, and the pro-apoptotic gene Bax was also affected at the 2-4-cell stage. RepSox treatment also increased the immunostaining intensity of Oct4 at the blastocyst stage (p < 0.05). Although the blastocyst developmental rate was higher in the group treated with 25 µM RepSox for 24 h than in the untreated control group (2.4 vs. 1.2%, p > 0.05), this was not significant.
  
  
  CONCLUSIONS
  RepSox can improve the developmental potential of rhesus monkey-pig iSCNT embryos by regulating the expression of pluripotency-related genes.
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhu
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Zhao-Bo
authors.initials: ZB
authors.lastname: Luo
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Li
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Zhang
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Xing
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Mei-Fu
authors.initials: MF
authors.lastname: Xuan
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Guang-Lei
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Qi-Rong
authors.initials: QR
authors.lastname: Luo
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Cheng-Du
authors.initials: CD
authors.lastname: Cui
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Wen-Xue
authors.initials: WX
authors.lastname: Li
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Zheng-Yun
authors.initials: ZY
authors.lastname: Cui
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China. yinxj33@msn.com.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China. yinxj33@msn.com.
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Yin
authors.affiliation: Department of Animal Science, Agricultural College, Yanbian University, Yanji, Jilin, 133002, China.|Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
authors.forename: Jin-Dan
authors.initials: JD
authors.lastname: Kang
mesh_terms: D000818:Animals; D019976:Cloning, Organism / Q000379:methods*; D004622:Embryo, Mammalian / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism; D008253:Macaca mulatta; D000071317:Nanog Homeobox Protein / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D050814:Octamer Transcription Factor-3; D009865:Oocytes / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology*; D013552:Swine
chemical_list: D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D011720:Pyrazoles; D011725:Pyridines; C550621:RepSox
keywords: Embryos (monkey–pig); Interspecies (monkey–pig) somatic cell nuclear transfer; Pluripotency; RepSox; Rhesus monkey; mRFP1