title: Enzyme-mediated ligation technologies for peptides and proteins.
abstract: With the steadily increasing complexity and quantity requirements for peptides in industry and academia, the efficient and site-selective ligation of peptides and proteins represents a highly desirable goal. Within this context, enzyme-mediated ligation technologies for peptides and proteins have attracted great interest in recent years as they represent an extremely powerful extension to the scope of chemical methodologies (e.g. native chemical ligation) in basic and applied research. Compared to chemical ligation methods, enzymatic strategies using ligases such as sortase, butelase, peptiligase or omniligase generally feature excellent chemoselectivity, therefore making them valuable tools for protein and peptide chemists.
authors.affiliation: EnzyPep B.V., Urmonderbaan 22, 6167RD Geleen, The Netherlands; Van't Hoff Institute of Molecular Sciences, University of Amsterdam, Science Park 904, 1098XH Amsterdam, The Netherlands.
authors.forename: Marcel
authors.initials: M
authors.lastname: Schmidt
authors.affiliation: EnzyPep B.V., Urmonderbaan 22, 6167RD Geleen, The Netherlands.
authors.forename: Ana
authors.initials: A
authors.lastname: Toplak
authors.affiliation: EnzyPep B.V., Urmonderbaan 22, 6167RD Geleen, The Netherlands.
authors.forename: Peter Jlm
authors.initials: PJ
authors.lastname: Quaedflieg
authors.affiliation: EnzyPep B.V., Urmonderbaan 22, 6167RD Geleen, The Netherlands. Electronic address: timo@enzypep.com.
authors.forename: Timo
authors.initials: T
authors.lastname: Nuijens
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004798:Enzymes; D010455:Peptides; D011506:Proteins