title: Dietary restriction reduces blood lipids and ameliorates liver function of mice with hyperlipidemia.
abstract: Dietary restriction (DR) can delay senescence, prolong lifespan of mammals and improve their learning-memory activity. The purpose of the study was to explore the effects of DR on hypolipidemic action and liver function of mice with hyperlipidemia. To investigate these effects, hyperlipidemia mouse models were established with high-fat diet (HFD) (34% of energy), then randomly divided into HFD group, DR30% group and DR50% group. Mice in DR30% and DR50% group were respectively supplied with HFD as much as about 70% and 50% of the consumption of HFD in the mice of HFD group. Rats in control group were fed routinely. After DR for 5 weeks, the average body weight, liver weight, liver index, serum lipids and glucose levels in both DR groups decreased significantly as compared with the HFD group (P<0.05 or P<0.01), so did alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) levels and the ratio of LDL-C/HDL-C in the DR50% group (P<0.05 or P<0.01). Histopathology examination of liver tissues further proved ameliorative effect of DR on liver function. Western blotting showed that DR significantly increased the expression of silent mating type information regulation 2 homolog 1 (SIRT1) in liver and adipose, while notably decreased the expression of peroxisome proliferators-activated receptors-gamma (PPARγ) in adipose (P<0.05 or P<0.01). The increase of SIRT1 and decrease of PPARγ may be a mechanism by which DR reduces blood lipids and ameliorates liver function.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Southeast University, Nanjing, 210009, China.|College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Gao
authors.affiliation: College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.|Institute of Molecular Medicine, Huaqiao University, Quanzhou, 362021, China.
authors.forename: Wen-Zhao
authors.initials: WZ
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Southeast University, Nanjing, 210009, China. q_xu68@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China. shaolinxiang@zjnu.cn.
authors.forename: Lin-Xiang
authors.initials: LX
authors.lastname: Shao
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001835:Body Weight; D031204:Caloric Restriction / Q000379:methods*; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D006949:Hyperlipidemias / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size; D047495:PPAR gamma / Q000378:metabolism; D011897:Random Allocation; D056564:Sirtuin 1 / Q000378:metabolism
chemical_list: D008055:Lipids; D047495:PPAR gamma; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: PPARγ; SIRT1; dietary restriction; high-fat diet; hyperlipidemia; hypolipidemic action; liver function