title: Evaluation of the interaction between hydroxyapatite and bisphosphonate by nonlinear capillary electrochromatography.
abstract: Bisphosphonates are a class of chemical compounds used to treat diseases caused by increased bone resorption. Zoledronate is a third-generation bisphosphonate drug. Hydroxyapatite is main mineral constituent of bones, which can be bound by bisphosphonates in vivo. In this work, we report a method of nonlinear capillary electrochromatography for study on the interaction between hydroxyapatite and bisphosphonate. Hydroxyapatite was modified on the inner wall of capillary by a biomimetic-mineralization method. Then nonlinear chromatography was used to fit and analyze the interaction between zoledronate and hydroxyapatite. The association rate constants of zoledronate in hydroxyapatite-modified capillary and bare capillary are 642.3 and 195/M/min, respectively. This indicates that there is strong binding interactions and affinity between zoledronate and hydroxyapatite. Besides, the interaction between zoledronate and hydroxyapatite was confirmed further by ultraviolet spectroscopy. The method of nonlinear capillary electrochromatography provides a fast and effect approach for studying of bone metabolism disease by evaluation of interaction between hydroxyapatite and bisphosphonates.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Deying
authors.initials: D
authors.lastname: Kong
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D001842:Bone and Bones; D053801:Capillary Electrochromatography; D004164:Diphosphonates / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D000077211:Zoledronic Acid
chemical_list: D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid; D017886:Durapatite
keywords: bisphosphonate; capillary electrochromatography; hydroxyapatite; nonlinear chromatography