title: Measuring Oxygen Consumption Rate inCaenorhabditis elegans.
abstract: The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaraset al., 2015; Ryuet al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chenet al., 2015; Vander Heidenet al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematodeCaenorhabditis elegans.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Palikaras
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece.
authors.forename: Nektarios
authors.initials: N
authors.lastname: Tavernarakis
keywords: Ageing; Caenorhabditis elegans; Metabolism; Mitochondria; Oxygen sensor; ROS