title: Tn6350, a Novel Transposon Carrying Pyocin S8 Genes Encoding a Bacteriocin with Activity against Carbapenemase-Producing Pseudomonas aeruginosa.
abstract: A novel transposon belonging to the Tn3-like family was identified on the chromosome of a commensal strain ofPseudomonas aeruginosasequence type 2343 (ET02). Tn6350is 7,367 bp long and harbors eight open reading frames (ORFs), an ATPase (IS481family), a transposase (DDE catalytic type), a Tn3resolvase, three hypothetical proteins, and genes encoding the new pyocin S8 with its immunity protein. We show that pyocin S8 displays activity against carbapenemase-producingP. aeruginosa, including IMP-1, SPM-1, VIM-1, GES-5, and KPC-2 producers.
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil helenagabrielaturano@hotmail.com lincopan@usp.br.
authors.forename: Helena
authors.initials: H
authors.lastname: Turano
authors.affiliation: Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gomes
authors.affiliation: Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Gesiele A
authors.initials: GA
authors.lastname: Barros-Carvalho
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Ralf
authors.initials: R
authors.lastname: Lopes
authors.affiliation: Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Louise
authors.initials: L
authors.lastname: Cerdeira
authors.affiliation: Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Luis E S
authors.initials: LES
authors.lastname: Netto
authors.affiliation: Laboratory Alerta, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Gales
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil helenagabrielaturano@hotmail.com lincopan@usp.br.|Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Nilton
authors.identifier: 0000-0003-0161-5800
authors.initials: N
authors.lastname: Lincopan
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D011709:Pyocins / Q000378:metabolism*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011709:Pyocins; C409201:beta-lactamase IMP-1; C491478:beta-lactamase KPC-2; C476155:beta-lactamase SPM-1, Pseudomonas aeruginosa; D001618:beta-Lactamases; C540661:beta-lactamase VIM-11, Pseudomonas aeruginosa; C063912:carbapenemase
keywords: Pseudomonas aeruginosa; S-type pyocins; bacteriocin; metallo-β-lactamases; transposon