title: Protective effect of hydroxytyrosol in arsenic-induced mitochondrial dysfunction in rat brain.
abstract: The present study was planned to investigate the protective effect of hydroxytyrosol (HT) against arsenic (As)-induced mitochondrial dysfunction in rat brain. Rats exposed to sodium arsenite (25 ppm for 8 weeks) showed decreased mitochondrial complexes (I, II, IV) activities, mitochondrial superoxide dismutase (MnSOD), and catalase activities in brain mitochondria. As-treated rats showed reduced mRNA expression of complex I (ND-1, ND-2), IV (COX-1, COX-4) subunits, and uncoupling protein-2 (UCP-2). In addition to this, As exposure downregulated the protein expression of MnSOD. Administration of HT with As restored the enzymatic activities of mitochondrial complexes, MnSOD and catalase, increased the mRNA levels of complexes subunits and UCP-2 as well as proteins level of MnSOD. These results suggest that HT efficiently restores mitochondrial dysfunction in As neurotoxicity and might be used as potential mitoprotective agent in future.
authors.affiliation: Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Soni
authors.affiliation: Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
authors.forename: Chandra
authors.initials: C
authors.lastname: Prakash
authors.affiliation: Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
authors.forename: Sfurti
authors.initials: S
authors.lastname: Sehwag
authors.affiliation: Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001928:Brain Diseases, Metabolic / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D024101:Mitochondrial Proteins; D009419:Nerve Tissue Proteins; C005975:3,4-dihydroxyphenylethanol; D010626:Phenylethyl Alcohol; D001151:Arsenic
keywords: arsenic; hydroxytyrosol; mitochondrial dysfunction; oxidative stress