title: Label-Free Method Using a Weighted-Phase Algorithm To Quantitate Nanoscale Interactions between Molecules on DNA Microarrays.
abstract: White light interference is used as a label-free method to detect nanoscale changes on surfaces. However, the signal-to-noise ratio of the white light interference method is very low, thus resulting in inaccurate results. In this paper, we report a corrected label-free method based on hyperspectral interferometry to overcome the shortcoming of the white light interference method. A platform based on hyperspectral interferometry was established, and a DNA hybridization microarray was constructed to quantitate thickness variation of molecules on a solid surface. We used fluorescence resonance energy transfer (FRET) to validate the results of our method. Compared to conventional fluorescence-labeled method like FRET, our method has advantages because it does not require a fluorescent label and has a detection limit of 1.78 nm, a high accuracy, and wide detection range (5-64 bp).
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Qi
authors.identifier: 0000-0002-4308-3411
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Rongxin
authors.initials: R
authors.lastname: Fu
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Junqi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Ruliang
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xue
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Su
authors.affiliation: National Engineering Research Center for Beijing Biochip Technology , Beijing 102206, PR China.
authors.forename: Wupeng
authors.initials: W
authors.lastname: Gan
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.|National Engineering Research Center for Beijing Biochip Technology , Beijing 102206, PR China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Huang
mesh_terms: D000465:Algorithms; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D007368:Interferometry; D008027:Light; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D004247:DNA