title: Proteomics in the Study of Bacterial Keratitis.
abstract: Bacterial keratitis is a serious ocular infection that can cause severe visual loss if treatment is not initiated at an early stage. It is most commonly caused byStaphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae,orSerratiaspecies. Depending on the invading organism, bacterial keratitis can progress rapidly, leading to corneal destruction and potential blindness. Common risk factors for bacterial keratitis include contact lens wear, ocular trauma, ocular surface disease, ocular surgery, lid deformity, chronic use of topical steroids, contaminated ocular medications or solutions, and systemic immunosuppression. The pathogenesis of bacterial keratitis, which depends on the bacterium-host interaction and the virulence of the invading bacterium, is complicated and not completely understood. This review highlights some of the proteomic technologies that have been used to identify virulence factors and the host response to infections of bacterial keratitis in order to understand the disease process and develop improved methods of diagnosis and treatment. Although work in this field is not abundant, proteomic technologies have provided valuable information toward our current knowledge of bacterial keratitis. More studies using global proteomic approaches are warranted because it is an important tool to identify novel targets for intervention and prevention of corneal damage caused by these virulent microorganisms.
authors.affiliation: Ophthalmology Department, Summa Health System, 525 East Market St, 274 Old Medical Building, Akron, OH 44304, USA. bouhennir@summahealth.org.
authors.forename: Rachida
authors.initials: R
authors.lastname: Bouhenni
authors.affiliation: Ophthalmology Department, Summa Health System, 525 East Market St, 274 Old Medical Building, Akron, OH 44304, USA. dunmirej@summahealth.org.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Dunmire
authors.affiliation: Ophthalmology Department, Summa Health System, 525 East Market St, 274 Old Medical Building, Akron, OH 44304, USA. batesj@summahealth.org.
authors.forename: Theresa
authors.initials: T
authors.lastname: Rowe
authors.affiliation: Ophthalmology Department, Summa Health System, 525 East Market St, 274 Old Medical Building, Akron, OH 44304, USA. batesj@summahealth.org.
authors.forename: James
authors.initials: J
authors.lastname: Bates
keywords: ELISA; antibody arrays; bacteria; keratitis; proteomics; western blotting