title: SlMAPK3 enhances tolerance to tomato yellow leaf curl virus (TYLCV) by regulating salicylic acid and jasmonic acid signaling in tomato (Solanum lycopersicum).
abstract: Several recent studies have reported on the role of mitogen-activated protein kinase (MAPK3) in plant immune responses. However, little is known about how MAPK3 functions in tomato (Solanum lycopersicum L.) infected with tomato yellow leaf curl virus (TYLCV). There is also uncertainty about the connection between plant MAPK3 and the salicylic acid (SA) and jasmonic acid (JA) defense-signaling pathways. The results of this study indicated that SlMAPK3 participates in the antiviral response against TYLCV. Tomato seedlings were inoculated with TYLCV to investigate the possible roles of SlMAPK1, SlMAPK2, and SlMAPK3 against this virus. Inoculation with TYLCV strongly induced the expression and the activity of all three genes. Silencing of SlMAPK1, SlMAPK2, and SlMAPK3 reduced tolerance to TYLCV, increased leaf H2O2 concentrations, and attenuated expression of defense-related genes after TYLCV infection, especially in SlMAPK3-silenced plants. Exogenous SA and methyl jasmonic acid (MeJA) both significantly induced SlMAPK3 expression in tomato leaves. Over-expression of SlMAPK3 increased the transcript levels of SA/JA-mediated defense-related genes (PR1, PR1b/SlLapA, SlPI-I, and SlPI-II) and enhanced tolerance to TYLCV. After TYLCV inoculation, the leaves of SlMAPK3 over-expressed plants compared with wild type plants showed less H2O2 accumulation and greater superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activity. Overall, the results suggested that SlMAPK3 participates in the antiviral response of tomato to TYLCV, and that this process may be through either the SA or JA defense-signaling pathways.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Tayeb
authors.initials: T
authors.lastname: Muhammad
authors.affiliation: College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Hehe
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
mesh_terms: D053839:Begomovirus / Q000502:physiology*; D003517:Cyclopentanes / Q000494:pharmacology*; D060467:Disease Resistance; D004790:Enzyme Induction; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000821:virology; D020935:MAP Kinase Signaling System; D048052:Mitogen-Activated Protein Kinase 3 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D010935:Plant Diseases / Q000235:genetics / Q000517:prevention & control* / Q000821:virology; D018515:Plant Leaves / Q000201:enzymology / Q000821:virology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D020156:Salicylic Acid / Q000494:pharmacology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D010088:Oxidoreductases; D048052:Mitogen-Activated Protein Kinase 3; D020156:Salicylic Acid