title: Prefabrication of Vascularized Allogenic Bone Graft in a Rat by Implanting a Flow-Through Vascular Pedicle and Basic Fibroblast Growth Factor Containing Hydroxyapatite/Collagen Composite.
abstract: BackgroundBasic fibroblast growth factor (bFGF) is known to stimulate bone formation and angiogenesis. Hydroxyapatite/collagen composite (HAp/Col) is also known to have very strong bone conductive activity. In this study, prefabrication of vascularized allogenic bone (allo-bone) graft was attempted in recipients by implanting vascular bundles from recipients into the transplanted allo-bone graft. Furthermore, the effect of bFGF-containing HAp/Col on the prefabricated vascularized allo-bone graft was investigated.MethodsIn this study, 32 Sprague-Dawley rats were used as donors, and bone grafts were collected from their femora. Thirty-two Wistar rats (recipients) were divided into four groups, and the allo-bone grafts were transplanted into the thigh region. In the experimental groups, one or both of the flow-through saphenous vascular bundles and 100-μg bFGF-containing HAp/Col were implanted into the medullary cavity of the allo-bone grafts. In the control group, neither was implanted. These rats were sacrificed at 4 weeks after transplantation, and bone formation, angiogenesis, and bone resorption in the transplanted allo-bone grafts were evaluated histologically and genetically.ResultsBone formation and angiogenesis in the transplanted allo-bone graft were effectively stimulated by implanting vascular bundles or bFGF-containing HAp/Col on both histological and genetic evaluations compared with the control group. The most significant stimulation was observed in the group in which both were implanted. Bone resorption was not stimulated in any group.ConclusionBy implanting a flow-through vascular bundle and bFGF-containing HAp/Col, an ideal vascularized allo-bone graft that had high bone formative and angiogenetic activities and did not stimulate bone resorptive activity was prefabricated.
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Konosuke
authors.initials: K
authors.lastname: Yamaguchi
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kaji
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakamura
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tobiume
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen; D004195:Disease Models, Animal; D017886:Durapatite; D005269:Femur / Q000187:drug effects / Q000637:transplantation*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism* / Q000494:pharmacology; D006085:Graft Survival / Q000187:drug effects; D020007:Implants, Experimental; D018919:Neovascularization, Physiologic; D016348:Osseointegration / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014184:Transplantation, Homologous
chemical_list: D055396:Bone Morphogenetic Protein 2; D016222:Fibroblast Growth Factor 2; D003094:Collagen; D017886:Durapatite