title: Partial-Body Irradiation in Patients with Prostate Cancer Treated with IMRT Has Little Effect on the Composition of Serum Proteome.
abstract: Partial body irradiation during cancer radiotherapy (RT) induces a response of irradiated tissues that could be observed at the level of serum proteome. Here we aimed to characterize the response to RT in group of patients treated because of prostate cancer. Five consecutive blood samples were collected before, during, and after the end of RT in a group of 126 patients who received definitive treatment with a maximum dose of 76 Gy. Serum peptidome, which was profiled in the 2000-16,000 Da range using MALDI-MS. Serum proteins were identified and quantified using the shotgun LC-MS/MS approach. The majority of changes in serum peptidome were detected between pre-treatment samples and samples collected after 3-4 weeks of RT (~25% of registered peptides changed their abundances significantly), yet the intensity of observed changes was not correlated significantly with the degree of acute radiation toxicity or the volume of irradiated tissues. Furthermore, there were a few serum proteins identified, the abundances of which were different in pre-RT and post-RT samples, including immunity and inflammation-related factors. Observed effects were apparently weaker than in comparable groups of head and neck cancer patients in spite of similar radiation doses and volumes of irradiated tissues in both groups. We concluded that changes observed at the level of serum proteome were low for this cohort of prostate cancer patients, although the specific components involved are associated with immunity and inflammation, and reflect the characteristic acute response of the human body to radiation.
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. m_pietrowska@io.gliwice.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Pietrowska
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. kjelonek@io.gliwice.pl.
authors.forename: Karol
authors.initials: K
authors.lastname: Jelonek
authors.affiliation: Institute of Automatics Control, Silesian University of Technology, Gliwice 44-100, Poland. joanna.polanska@polsl.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Polanska
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. awojakowska@io.gliwice.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Wojakowska
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań 61-704, Poland. lukasmar@ibch.poznan.pl.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Marczak
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. ewa.chawinska@io.gliwice.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Chawinska
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. aleksandra.chmura@io.gliwice.pl.
authors.forename: Aleksanda
authors.initials: A
authors.lastname: Chmura
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. wmajewski1@poczta.onet.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Majewski
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. leszek@io.gliwice.pl.
authors.forename: Leszek
authors.initials: L
authors.lastname: Miszczyk
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice 44-101, Poland. widlak@io.gliwice.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Widlak
keywords: IMRT; mass spectrometry; prostate cancer; radiation response; serum proteomics