title: Roselle supplementation prevents nicotine-induced vascular endothelial dysfunction and remodelling in rats.
abstract: Vascular endothelial dysfunction (VED) plays an important role in the initiation of cardiovascular diseases. Roselle, enriched with antioxidants, demonstrates high potential in alleviating hypertension. This study was undertaken to investigate the effects of roselle supplementation of VED and remodelling in a rodent model with prolonged nicotine administration. Male Sprague-Dawley rats (n = 6 per group) were administered with 0.6 mg/kg nicotine for 28 days to induce VED. The rats were given either aqueous roselle (100 mg/kg) or normal saline orally 30 min prior to nicotine injection daily. One additional group of rats served as control. Thoracic aorta was isolated from rats to measure vascular reactivity, vascular remodelling and oxidative stress. Roselle significantly lowered aortic sensitivity to phenylephrine-induced vasoconstriction (Endo-(+) Cmax= 234.5 ± 3.9%, Endo-(-) Cmax= 247.6 ± 5.2%) compared with untreated nicotine group (Endo-(+) Cmax= 264.5 ± 6.9%, Endo-(-) Cmax= 276.5 ± 6.8%). Roselle also improved aortic response to endothelium-dependent vasodilator, acetylcholine (Endo-(+) Rmax= 73.2 ± 2.1%, Endo-(-) Rmax= 26.2 ± 0.8%) compared to nicotine group (Endo-(+) Rmax= 57.8 ± 1.7%, Endo-(-) Rmax= 20.9 ± 0.8%). In addition, roselle prevented an increase in intimal media thickness and elastic lamellae proliferation to preserve vascular architecture. Moreover, we also observed a significantly lowered degree of oxidative stress in parallel with increased antioxidant enzymes in aortic tissues of the roselle-treated group. This study demonstrated that roselle prevents VED and remodelling, and as such it has high nutraceutical value as supplement to prevent cardiovascular diseases.
authors.affiliation: a Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Lislivia Yiang-Nee
authors.initials: LY
authors.lastname: Si
authors.affiliation: b Department of Pharmacology, Faculty of Medicine, Jalan Yaacob Latif, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.
authors.forename: Yusof
authors.initials: Y
authors.lastname: Kamisah
authors.affiliation: a Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Anand
authors.initials: A
authors.lastname: Ramalingam
authors.affiliation: a Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Yi Cheng
authors.initials: YC
authors.lastname: Lim
authors.affiliation: a Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Siti Balkis
authors.initials: SB
authors.lastname: Budin
authors.affiliation: a Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Satirah
authors.initials: S
authors.lastname: Zainalabidin
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001013:Aorta, Thoracic; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D031584:Hibiscus / Q000737:chemistry*; D008297:Male; D009538:Nicotine / Q000008:administration & dosage / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects; D010656:Phenylephrine / Q000494:pharmacology; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014652:Vascular Diseases / Q000139:chemically induced / Q000517:prevention & control*; D014661:Vasoconstriction / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D028321:Plant Preparations; D014665:Vasodilator Agents; D010656:Phenylephrine; D009538:Nicotine; D000109:Acetylcholine
keywords: dysfonction endothéliale; endothelial dysfunction; hypertension; nicotine; oxidative stress; remodelage vasculaire; roselle; stress oxydatif; vascular remodelling