title: The Integrins Involved in Soybean Agglutinin-Induced Cell Cycle Alterations in IPEC-J2.
abstract: Soybean agglutinin (SBA) is an anti-nutritional factor of soybean, affecting cell proliferation and inducing cytotoxicity. Integrins are transmembrane receptors, mediating a variety of cell biological processes. This research aims to study the effects of SBA on cell proliferation and cell cycle progression of the intestinal epithelial cell line from piglets (IPEC-J2), to identify the integrin subunits especially expressed in IPEC-J2s, and to analyze the functions of these integrins on IPEC-J2 cell cycle progression and SBA-induced IPEC-J2 cell cycle alteration. The results showed that SBA lowered cell proliferation rate as the cell cycle progression from G0/G1 to S phase (P< 0.05) was inhibited. Moreover, SBA lowered mRNA expression of cell cycle-related gene CDK4, Cyclin E and Cyclin D1 (P< 0.05). We successfully identified integrins α2, α3, α6, β1, and β4 in IPEC-J2s. These five subunits were crucial to maintain normal cell proliferation and cell cycle progression in IPEC-J2s. Restrain of either these five subunits by their inhibitors, lowered cell proliferation rate, and arrested the cells at G0/G1 phase of cell cycle (P< 0.05). Further analysis indicated that integrin α2, α6, and β1 were involved in the blocking of G0/G1 phase induced by SBA. In conclusion, these results suggested that SBA lowered the IPEC-J2 cell proliferation rate through the perturbation of cell cycle progression. Furthermore, integrins were important for IPEC-J2 cell cycle progression, and they were involved in the process of SBA-induced cell cycle progression alteration, which provide a basis for further revealing SBA anti-proliferation and anti-nutritional mechanism.
authors.affiliation: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.affiliation: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China.|Animal production Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt.
authors.forename: Mohammed Hamdy
authors.initials: MH
authors.lastname: Farouk
authors.affiliation: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China.
authors.forename: Shiyao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Bao
authors.affiliation: Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China.
authors.forename: Guixin
authors.initials: G
authors.lastname: Qin
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D016023:Integrins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D037121:Plant Lectins / Q000494:pharmacology*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D012844:Sincalide / Q000032:analysis / Q000378:metabolism; D030262:Soybean Proteins / Q000494:pharmacology*; D013552:Swine; D010835:Phytohemagglutinins
chemical_list: D016023:Integrins; D037121:Plant Lectins; D012844:Sincalide; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins
keywords: cell cycle; cell proliferation; functional mechanism; integrin subunits; soybean agglutinin