title: Oscillatory Reaction Induced Periodic C-Quadruplex DNA Gating of Artificial Ion Channels.
abstract: Many biological ion channels controlled by biochemical reactions have autonomous and periodic gating functions, which play important roles in continuous mass transport and signal transmission in living systems. Inspired by these functional biological ion channel systems, here we report an artificial self-oscillating nanochannel system that can autonomously and periodically control its gating process under constant conditions. The system is constructed by integrating a chemical oscillator, consisting of BrO3-, Fe(CN)64-, H+, and SO32-, into a synthetic proton-sensitive nanochannel modified with C-quadruplex (C4) DNA motors. The chemical oscillator, containing H+-producing and H+-consuming reactions, can cyclically drive conformational changes of the C4-DNA motors on the channel wall between random coil and folded i-motif structures, thus leading to autonomous gating of the nanochannel between open and closed states. The autonomous gating processes are confirmed by periodic high-low ionic current oscillations of the channel monitored under constant reaction conditions. The utilization of a chemical oscillator integrated with DNA molecules represents a method to directly convert chemical energy of oscillating reactions to kinetic energy of conformational changes of the artificial nanochannels and even to achieve diverse autonomous gating functions in artificial nanofluidic devices.
authors.affiliation: Key Laboratory of Bio-inspired Materials and Interface Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University , Beijing 100084, People's Republic of China.
authors.forename: Ruochen
authors.initials: R
authors.lastname: Fang
authors.affiliation: Department of Chemical Engineering, Monash University , Clayton, Victoria 3800, Australia.
authors.forename: Jue
authors.identifier: 0000-0003-0325-6979
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Chemical Engineering, Monash University , Clayton, Victoria 3800, Australia.
authors.forename: Huacheng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Chemical Engineering, Monash University , Clayton, Victoria 3800, Australia.
authors.forename: Huanting
authors.identifier: 0000-0002-9887-5555
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-inspired Materials and Interface Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.|Department of Chemical Engineering, Monash University , Clayton, Victoria 3800, Australia.
authors.forename: Lei
authors.identifier: 0000-0003-4579-728X
authors.initials: L
authors.lastname: Jiang
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism; D001959:Bromates / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D009991:Oscillometry; D011522:Protons; D013447:Sulfites / Q000737:chemistry / Q000378:metabolism
chemical_list: D001959:Bromates; D005295:Ferrocyanides; D007473:Ion Channels; D011522:Protons; D013447:Sulfites; D004247:DNA
keywords: C-quadruplex DNA; artificial nanochannel; conformational changes; gating; self-oscillating