title: Enzymatic in situ formed hydrogel from gelatin-tyramine and chitosan-4-hydroxylphenyl acetamide for the co-delivery of human adipose-derived stem cells and platelet-derived growth factor towards vascularization.
abstract: An injectable, in situ forming hydrogel system capable of co-delivering human adipose-derived stem cells (hADSC) and platelet-derived growth factor (PDGF) was investigated as a new system for tissue engineering, envisaged to support vascularization. The system consists of tyramine-conjugated gelatin and hydroxyphenyl acetamide chitosan derivative. Both are soluble and stable at physiologic conditions, which is a key factor for retaining viable cells and active growth factor. In situ gelation involved enzymatic crosslinking using horseradish peroxidase as a catalyst and hydrogen peroxide as an oxidant. Gel formation occurred within 30-90 s by controlling the concentration of polymers. PDGF release showed adequate release kinetics within the intended period of time and hADSC showed good compatibility with the hydrogel formulation based on the in vitro assay and subcutaneous implantation into BALB/c-nu/nu nude female mice. Immunohistochemical analysis confirmed viability of delivered hADSC. Histological analysis showed no immune reaction and confirmed blood vessel formation. The results implicate the hydrogel as a promising delivery vehicle or carrier of both cell and growth factor, which support vascularization for tissue engineering applications.
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, 330-090, Republic of Korea. Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, 330-090, Republic of Korea.
authors.forename: Nguyen Thuy Ba
authors.initials: NT
authors.lastname: Linh
authors.forename: Celine D G
authors.initials: CD
authors.lastname: Abueva
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D000081:Acetamides / Q000737:chemistry; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D000077214:Becaplermin; D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D016503:Drug Delivery Systems; D005260:Female; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage*; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014439:Tyramine / Q000737:chemistry*
chemical_list: D000081:Acetamides; D001672:Biocompatible Materials; D020100:Hydrogels; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D005780:Gelatin; D048271:Chitosan; D014439:Tyramine