title: Development of a highly efficient oil degumming process using a novel phosphatidylinositol-specific phospholipase C enzyme.
abstract: Enzymatic degumming using phospholipase C (PLC) enzymes may be used in environmentally friendly processes with improved oil recovery yields. In this work, phosphatidylinositol-specific phospholipase C (PIPLC) candidates obtained from an in silico analysis were evaluated for oil degumming. A PIPLC from Lysinibacillus sphaericus was shown to efficiently remove phosphatidylinositol from crude oil, and when combined with a second phosphatidylcholine and phosphatidylethanolamine-specific phospholipase C, the three major phospholipids were completely hydrolyzed, providing an extra yield of oil greater than 2.1%, compared to standard methods. A remarkably efficient fed-batch Escherichia coli fermentation process producing ∼14 g/L of the recombinant PIPLC enzyme was developed, which may facilitate the adoption of this cost-effective oil-refining process.
authors.affiliation: CONICET y Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Cerminati
authors.affiliation: CONICET y Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Eberhardt
authors.affiliation: Keclon S.A., Tucumán 7180, 2000, Rosario, Argentina.
authors.forename: Claudia E
authors.initials: CE
authors.lastname: Elena
authors.affiliation: CONICET y Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Suipacha 531, 2000, Rosario, Argentina.|Keclon S.A., Tucumán 7180, 2000, Rosario, Argentina.
authors.forename: Salvador
authors.initials: S
authors.lastname: Peirú
authors.affiliation: CONICET y Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Suipacha 531, 2000, Rosario, Argentina.|Keclon S.A., Tucumán 7180, 2000, Rosario, Argentina.
authors.forename: María E
authors.initials: ME
authors.lastname: Castelli
authors.affiliation: CONICET y Departamento de Tecnología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ), Suipacha 531, 2000, Rosario, Argentina. hmenzella@fbioyf.unr.edu.ar.|Keclon S.A., Tucumán 7180, 2000, Rosario, Argentina. hmenzella@fbioyf.unr.edu.ar.
authors.forename: Hugo G
authors.initials: HG
authors.lastname: Menzella
mesh_terms: D001406:Bacillaceae / Q000201:enzymology* / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D007700:Kinetics; D010578:Petroleum / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism
chemical_list: D010578:Petroleum; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D010938:Plant Oils; D011994:Recombinant Proteins; C483858:phosphatidylethanolamine; D054801:Phosphoinositide Phospholipase C
keywords: Enzymatic degumming; Green chemistry; Phosphatidylinositol phospholipase C; Phospholipid removal