title: Image-based adaptive optics for in vivo imaging in the hippocampus.
abstract: Adaptive optics is a promising technique for the improvement of microscopy in tissues. A large palette of indirect and direct wavefront sensing methods has been proposed for in vivo imaging in experimental animal models. Application of most of these methods to complex samples suffers from either intrinsic and/or practical difficulties. Here we show a theoretically optimized wavefront correction method for inhomogeneously labeled biological samples. We demonstrate its performance at a depth of 200 μm in brain tissue within a sparsely labeled region such as the pyramidal cell layer of the hippocampus, with cells expressing GCamP6. This method is designed to be sample-independent thanks to an automatic axial locking on objects of interest through the use of an image-based metric that we designed. Using this method, we show an increase of in vivo imaging quality in the hippocampus.
authors.affiliation: INSERM UMR901 INMED, Institut de Neurobiologie de la Méditerranée, Aix-Marseille Université, 13273 Marseille, France.|Aix-Marseille Université, CNRS, Centrale Marseille, Institut Fresnel UMR 7249, 13013 Marseille, France.
authors.forename: D
authors.initials: D
authors.lastname: Champelovier
authors.affiliation: Onera - the French Aerospace Lab, F-92322 Châtillon, France.
authors.forename: J
authors.initials: J
authors.lastname: Teixeira
authors.affiliation: Onera - the French Aerospace Lab, F-92322 Châtillon, France.
authors.forename: J-M
authors.initials: JM
authors.lastname: Conan
authors.affiliation: Aix-Marseille Université, CNRS, Centrale Marseille, Institut Fresnel UMR 7249, 13013 Marseille, France.
authors.forename: N
authors.initials: N
authors.lastname: Balla
authors.affiliation: Onera - the French Aerospace Lab, F-92322 Châtillon, France.
authors.forename: L M
authors.initials: LM
authors.lastname: Mugnier
authors.affiliation: INSERM UMR901 INMED, Institut de Neurobiologie de la Méditerranée, Aix-Marseille Université, 13273 Marseille, France.
authors.forename: T
authors.initials: T
authors.lastname: Tressard
authors.affiliation: INSERM UMR901 INMED, Institut de Neurobiologie de la Méditerranée, Aix-Marseille Université, 13273 Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Reichinnek
authors.affiliation: Onera - the French Aerospace Lab, F-92322 Châtillon, France.
authors.forename: S
authors.initials: S
authors.lastname: Meimon
authors.affiliation: INSERM UMR901 INMED, Institut de Neurobiologie de la Méditerranée, Aix-Marseille Université, 13273 Marseille, France.
authors.forename: R
authors.initials: R
authors.lastname: Cossart
authors.affiliation: Aix-Marseille Université, CNRS, Centrale Marseille, Institut Fresnel UMR 7249, 13013 Marseille, France.
authors.forename: H
authors.initials: H
authors.lastname: Rigneault
authors.affiliation: Aix-Marseille Université, CNRS, Centrale Marseille, Institut Fresnel UMR 7249, 13013 Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Monneret
authors.affiliation: INSERM UMR901 INMED, Institut de Neurobiologie de la Méditerranée, Aix-Marseille Université, 13273 Marseille, France.|Aix-Marseille Université, CNRS, Centrale Marseille, Institut Fresnel UMR 7249, 13013 Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Malvache
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000000981:diagnostic imaging*; D021621:Imaging, Three-Dimensional; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton
chemical_list: D049452:Green Fluorescent Proteins; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1