title: Bioenergetic Analyses in Adipose Tissue.
abstract: Adipose tissue plays an important role in whole body metabolism, and its dysfunction results in poor metabolic health and is associated with a diverse set of disorders, such as obesity, cardiovascular disease, hypertension, cancer, and type 2 diabetes. The search for cellular processes that can be targeted to activate adipose oxidative metabolism is thought to hold promise for combating these diseases, and so the analysis of adipose bioenergetics is crucial toward this end. A description is provided here for the preparation of mitochondria and primary adipocytes from adipose tissue to be used for bioenergetics profiling.
authors.affiliation: Dana-Faber Cancer Institute, Harvard Medical School, Cancer Biology CLS 111146, 3 Blackfan Circle, Boston, MA, 02115, USA. lawrence_kazak@dfci.harvard.edu.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Kazak
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002458:Cell Fractionation / Q000379:methods; D002469:Cell Separation / Q000379:methods; D004734:Energy Metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
keywords: Beige fat; Bioenergetics; Brown fat; Mitochondria; Primary adipocytes; Stromal vascular fraction