title: Effect of acetate as a co-feedstock on the production of poly(lactate-co-3-hydroxyalkanoate) by pflA-deficient Escherichia coli RSC10.
abstract: Developing Escherichia coli strains that are tolerant to acetate toxicity is important in light of an increased interest in the efficient utilization of lignocellulosic biomass feedstocks for the biosynthesis of value-added products. In this study, four strains known to produce polyhydroxyalkanoates (PHAs) from the typical hemicellulosic sugar xylose were tested for their tolerance to acetate. E. coli RSC10 was found to be tolerant of acetate, both in growth and fermentation studies. In the presence of acetate the strain showed a >2-fold increase in overall yields compared to using xylose alone as the feedstock. More importantly, the strain was found to be able to utilize acetate as a feedstock for biosynthesis of PHAs, with complete depletion of acetate (25 mM) at 9 h when acetate was the sole feedstock. Higher concentrations of acetate showed greater inhibition of fermentation than growth with a reduction of 90% in PHA yields at 100 mM. Additionally, the present work provides data to support the potential of acetate as a modulator for the control of composition of PHAs that incorporate lactate (LA) monomers into the copolymer from hemicellulose derived sugars.
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Lucia
authors.initials: L
authors.lastname: Salamanca-Cardona
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Scheel
authors.affiliation: Department of Creative Engineering, National Institute of Technology, Kitakyushu College, 5-20-1 Shii, Kokuraminami-ku, Kitakyushu 802-0985, Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Mizuno
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: N Scott
authors.initials: NS
authors.lastname: Bergey
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Stipanovic
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-28, Kita-ku, Sapporo 060-8638, Japan.
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-28, Kita-ku, Sapporo 060-8638, Japan; CREST, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
authors.affiliation: Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA; Center for Applied Microbiology, State University of New York, College of Environmental Science and Forestry, Syracuse, NY 13210, USA; Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: ctnomura@esf.edu.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Nomura
mesh_terms: D000085:Acetates / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005285:Fermentation / Q000187:drug effects; D019344:Lactic Acid / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis; D011134:Polysaccharides / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
chemical_list: D000085:Acetates; D029968:Escherichia coli Proteins; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D011134:Polysaccharides; D019344:Lactic Acid; C007916:hemicellulose; D014994:Xylose
keywords: Acetate; Biomass; Hemicellulose; Polyhydroxyalkanoate; Recombinant Escherichia coli; Xylose