title: Rapid immunoassay exploiting nanoparticles and micromagnets: proof-of-concept using ovalbumin model.
abstract: OBJECTIVE
  We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40-2500 ng/ml) similar to that of ELISAs developed using same biochemical tools.
  
  
  CONCLUSIONS
  Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.
authors.affiliation: INSERM U1209, F-38000 Grenoble, France.|University Grenoble Alpes, IAB, F-38000 Grenoble, France.|Univ. Grenoble Alpes, CNRS, Grenoble INP†, G2Elab, F-38000 Grenoble, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Delshadi
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, G2Elab, F-38000 Grenoble, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Blaire
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, G2Elab, F-38000 Grenoble, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Kauffmann
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, G2Elab, F-38000 Grenoble, France.|Univ. Grenoble Alpes, CNRS, Grenoble INP†, Néel Institute, F-38000 Grenoble, France.
authors.forename: Mario
authors.initials: M
authors.lastname: Fratzl
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, Néel Institute, F-38000 Grenoble, France.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Devillers
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, LMGP, F-38000 Grenoble, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Delabouglise
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, LMGP, F-38000 Grenoble, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Weidenhaupt
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, Néel Institute, F-38000 Grenoble, France.
authors.forename: Nora M
authors.initials: NM
authors.lastname: Dempsey
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, G2Elab, F-38000 Grenoble, France.
authors.forename: Orphée
authors.initials: O
authors.lastname: Cugat
authors.affiliation: Univ. Grenoble Alpes, CNRS, Grenoble INP†, LMGP, F-38000 Grenoble, France.
authors.forename: Franz
authors.initials: F
authors.lastname: Bruckert
authors.affiliation: INSERM U1209, F-38000 Grenoble, France.|University Grenoble Alpes, IAB, F-38000 Grenoble, France.
authors.forename: Patrice N
authors.initials: PN
authors.lastname: Marche
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D060526:Magnetic Fields; D059346:Magnets / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010047:Ovalbumin / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D010047:Ovalbumin
keywords: ELISA; colorimetry; diagnostic; immunoassay; magnetism; method development; nanoparticles; ovalbumin