title: Effects of in ovo feeding of creatine pyruvate on the hatchability, growth performance and energy status in embryos and broiler chickens.
abstract: The effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the hatchability, growth performance and energy status of embryos and broilers (Arbor Acres) were investigated. Five treatments were arranged as non-injected treatment (Control), 0.6 ml physiological saline (0.75%) injected treatment (Saline), and IOF treatments injected with 0.6 ml physiological saline (0.75%) containing 3, 6 or 12 mg CrPyr (CrPyr3, CrPyr6 or CrPyr12) into the amnion per fertile egg on day 17.5 of incubation. After hatching, 80 male chicks from each treatment with similar weight close to the average BW of their pooled group were selected and randomly assigned into eight replicates of 10 chicks each. The results showed that the hatchability was not affected among groups, whereas the hatching weight of broilers in CrPyr12 was significantly higher than the control and saline groups (P0.05). Irrespective of dosage, the concentrations of creatine and phosphocreatine, and activities of creatine kinase in embryos were enhanced in CrPyr treatments at 19 E when compared with the control and saline groups (P<0.05). The activities of glucose-6-phosphatase in liver in CrPyr6 and CrPyr12 treatments were higher than the control and saline groups at 19 E (P<0.05). In conclusion, these results indicated that IOF of CrPyr, especially at the level of 12 mg/egg, could improve energy status of embryos and hatchlings, which was useful for enhancing hatching weight, BW and pectoral muscle weight until the end of the experiments at 21 days post-hatch in broilers.
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: M M
authors.initials: MM
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: T
authors.initials: T
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: J L
authors.initials: JL
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: P A
authors.initials: PA
authors.lastname: Lv
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: L L
authors.initials: LL
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: F
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
authors.forename: G H
authors.initials: GH
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000254:growth & development / Q000502:physiology*; D002645:Chickens / Q000254:growth & development / Q000502:physiology*; D003401:Creatine / Q000378:metabolism*; D003402:Creatine Kinase / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*
chemical_list: D010725:Phosphocreatine; D019289:Pyruvic Acid; D003402:Creatine Kinase; D003401:Creatine
keywords: broilers; creatine pyruvate; energy status; growth performance; in ovo feeding