title: Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells.
abstract: Recently, molecular imaging has become a conditio sine qua non for cell-based regenerative medicine. Developments in molecular imaging techniques, such as reporter gene technology, have increasingly enabled the noninvasive assessment of the fate and biology of cells after cardiovascular applications. In this context, bioluminescence imaging is the most commonly used imaging modality in small animal models of preclinical studies. Here, we present a detailed protocol of a reporter gene imaging approach for monitoring the viability and biology of Mesenchymal Stem Cells transplanted in a mouse model of myocardial ischemia reperfusion injury.
authors.affiliation: Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St. SW, Rochester, 55905, MN, USA.
authors.forename: Federico
authors.initials: F
authors.lastname: Franchi
authors.affiliation: Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St. SW, Rochester, 55905, MN, USA. rodriguez.m@mayo.edu.
authors.forename: Martin
authors.initials: M
authors.lastname: Rodriguez-Porcel
mesh_terms: D000818:Animals; D058948:Cell Tracking; D004195:Disease Models, Animal; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D012190:Retroviridae / Q000235:genetics; D014161:Transduction, Genetic
keywords: Bioluminescence; Heart; Luciferase; Molecular imaging; Monitoring; Reporter gene; Stem cells; Tracking