title: [Diagnostic Significance of BIOMED-2 Standardized Gene Rearran-gement System in Patients with Non-Hodgkin's Lymphoma].
abstract: OBJECTIVE
  To detect the immunoglobulin(Ig) and T cell receptor(TCR) gene rearrangement in bone marrow of non-Hodgkin's lymphoma(NHL) patients by using BIOMED-2 standardized system, and to explore the potential clinical significance of Ig/TCR gene rearrangement.
  
  
  METHODS
  DNA was extracted in bone marrow and Formalin-fixed and Paraffin-embedded(FFPE) samples of NHL patients, the Ig/TCR gene rearrangements were analyzed by using BIOMED-2 multiple primers system and multiplex PCR assay.
  
  
  RESULTS
  Among 235 T-NHL cases, 71.9% showed TCR gene rearrangement. The positive rate of TCRγ and the TCRβ were 57.9% and 50.2%. Out of 583 B-NHL cases, 81.6% showed Ig gene rearrangement. The positive rate of IgH and the IgK were 70.7% and 69.3%. MCL patients showed 84.8% IgH rearrangement and 75.8% IgK rearrangement, as compared with FL(34.0%, 50.9%) and DLBCL(9.2%, 16.1%) patients, the difference was statistically significant (P<0.05). Out of Ig rearrangement positive B-NHL cases, 65 showed TCR gene rearrangement. None TCR rearrangement positive T-NHL cases showed Ig gene rearrangement, 25 cases(83.3%) showed Ig gene rearrangement in FFPE samples of 30 DLBCL patients, as compared to Ig rearrangement positive rate of bone marrow, the difference was statistically significant (P<0.001).
  
  
  CONCLUSIONS
  BIOMED-2 standardized Ig/TCR gene rearrangement system shows assistance for lymphoma diagnosis. The PCR sequencing analysis is much more sensitive and specific and has significance for clinical diagnosis.
authors.affiliation: Center of Pathology, Institute of Hematology & Blood Disease Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center of Pathology, Institute of Hematology & Blood Disease Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Examination Medicine, Tianjin Hospital, Tianjin 300211, China.
authors.forename: Bao-Jun
authors.initials: BJ
authors.lastname: Dong
authors.affiliation: Center of Pathology, Institute of Hematology & Blood Disease Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Song
authors.affiliation: Center of Pathology, Institute of Hematology & Blood Disease Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Li
authors.affiliation: Center of Pathology, Institute of Hematology & Blood Disease Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Gang
authors.initials: G
authors.lastname: An
authors.affiliation: Center of Pathology, Institute of Hematology & Blood Disease Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail: irvine_422@126.com.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Ai
mesh_terms: D017931:DNA Primers; D015321:Gene Rearrangement; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis* / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000276:immunology; D011948:Receptors, Antigen, T-Cell
chemical_list: D017931:DNA Primers; D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell