title: Variation in Campylobacter Multilocus Sequence Typing Subtypes from Chickens as Detected on Three Plating Media.
abstract: The objective of this study was to compare subtypes of Campylobacter jejuni and Campylobacter coli detected on three selective Campylobacter plating media to determine whether each medium selected for different subtypes. Fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. Campylobacter was cultured and isolated from cecal contents and carcass rinses on Campy-Cefex, Campy Line, and RF Campylobacter jejuni/coli agars. When a positive result was obtained with all three media, one colony of the most prevalent morphology on each medium was selected for further analysis by full genome sequencing and multilocus sequence typing. Sequence types were assigned according to PubMLST. A total of 49 samples were positive for Campylobacter on all three media. Forty samples contained only C. jejuni , three had only C. coli , and both species were detected in six samples. From 71% of samples, Campylobacter isolates of the same sequence type were recovered on all three media. From 81.6% of samples, isolates were all from the same clonal complex. From significantly fewer samples (26%, P < 0.01), one medium recovered an isolate with a sequence type different from the type recovered on the other two media. When multiple sequence types were detected, six times the medium with the odd sequence type was Campy-Cefex, four times it was Campy-Line, and six times it was RF Campylobacter jejuni/coli . From one sample, three sequence types were detected. In most cases, all three plating media allowed detection of the same type of Campylobacter from complex naturally contaminated chicken samples.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Athens, Georgia 30505.
authors.forename: M E
authors.initials: ME
authors.lastname: Berrang
authors.affiliation: U.S. Department of Agriculture, Food Safety Inspection Service, Athens, Georgia 30605.
authors.forename: S R
authors.initials: SR
authors.lastname: Ladely
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Athens, Georgia 30505.
authors.forename: R J
authors.initials: RJ
authors.lastname: Meinersmann
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Athens, Georgia 30505.
authors.forename: J E
authors.initials: JE
authors.lastname: Line
authors.affiliation: College of Veterinary Medicine, Western University of Health Sciences, Pomona, California 91766, USA.
authors.forename: B B
authors.initials: BB
authors.lastname: Oakley
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Athens, Georgia 30505.
authors.forename: N A
authors.initials: NA
authors.lastname: Cox
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000235:genetics*; D017000:Campylobacter coli / Q000235:genetics; D016123:Campylobacter jejuni / Q000235:genetics; D002645:Chickens; D058885:Multilocus Sequence Typing
keywords: Broiler; Campylobacter; Chicken; Multilocus sequence typing; Plating media