title: Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli.
abstract: Monoclonal antibodies (mAbs) against black rockfish Sebastes schlegeli serum immunoglobulin M (IgM) were developed, which showed a specific reaction with the heavy chain of S. schlegeli IgM in Western blotting and with surface IgM positive (sIgM+) lymphocytes in indirect immunofluorescence. mAb 2A6 was employed to investigate the antibody and sIgM+lymphocyte responses of S. schlegeli injected with inactivated Edwardsiella tarda, by ELISA and flow cytometry. Compared with controls, the level of specific antibodies and the percentage of sIgM+lymphocytes both increased in the immunized fish and simultaneously reached their peaks at day 35 after immunization.
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, P. R. China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, P. R. China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Tang
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, P. R. China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Sheng
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, P. R. China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao, China.
authors.forename: J
authors.initials: J
authors.lastname: Xing
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, P. R. China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao, China.
authors.forename: W B
authors.initials: WB
authors.lastname: Zhan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015415:Biomarkers; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007075:Immunoglobulin M / Q000276:immunology*; D010473:Perciformes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007075:Immunoglobulin M
keywords: Edwardsiella tarda; flow cytometry; immune response; sIgM+ lymphocytes