title: CDX2 is essential for cell proliferation and polarity in porcine blastocysts.
abstract: The role of CDX2 in trophectoderm (TE) cells has been extensively studied, yet the results are contradictory and species specific. Here, CDX2 expression and function were explored in early porcine embryos. Notably, siRNA-mediated gene knockdown and lentivirus-mediated TE-specific gene regulation demonstrated that CDX2 is essential for the maintenance of blastocyst integrity by regulating the BMP4-mediated blastocyst niche and classic protein kinase C (PKC)-mediated TE polarity in mammalian embryos. Mechanistically,CDX2-depleted porcine embryos stalled at the blastocyst stage and exhibited apoptosis and inactive cell proliferation, possibly resulting from BMP4 downregulation. Moreover, TE cells inCDX2-depleted blastocysts displayed defective F-actin apical organization associated with downregulation of PKCα (PRKCA). Collectively, these results provide further insight into the functional diversity of CDX2 in early mammalian embryos.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.|College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China.
authors.forename: Gerelchimeg
authors.initials: G
authors.lastname: Bou
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Shichao
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Mingju
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Xue
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Life Science and Biotechnique Research Center, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qu
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Weng
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yanchang
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin 150030, China liuzhonghua@neau.edu.cn liu086@yahoo.com.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China.
authors.forename: Zhonghua
authors.identifier: 0000-0002-9199-6493
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism*; D001757:Blastomeres / Q000166:cytology / Q000378:metabolism; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D000071616:CDX2 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D016764:Cell Polarity; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D004475:Ectoderm / Q000196:embryology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D051571:Protein Kinase C-alpha / Q000378:metabolism; D034421:Sus scrofa; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics
chemical_list: D055415:Bone Morphogenetic Protein 4; D000071616:CDX2 Transcription Factor; D051571:Protein Kinase C-alpha
keywords: BMP4; CDX2; Cell proliferation; PKCα; TE polarity