title: Analysis of distinct long noncoding RNA transcriptional fingerprints in pancreatic ductal adenocarcinoma.
abstract: Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies with the worst prognosis. Recent studies have demonstrated that long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis and cancer progression. However, the expression pattern and roles of lncRNAs in the development of PDAC remain unknown. Herein, we globally analyzed the lncRNA expression profile in human PDAC and non-tumor tissues using four independent public microarray datasets from Gene Expression Omnibus (GEO). The analysis of GEO datasets by repurposing microarray probes confirmed that hundreds of lncRNAs are differentially expressed in PDAC tissues compared with normal tissues. We selected four lncRNAs including LINC00152, CASC9, LINC00226 and F11-AS1 for validation in PDAC cell lines and normal cells. Loss of function assays were performed to investigate the roles of LINC00152 and CASC9 in PDAC cell proliferation and invasion. Taken together, our findings demonstrate lncRNA expression alterations in PDAC and may provide new potential molecular markers for PDAC patient diagnosis and treatment.
authors.affiliation: Department of General Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of General Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of General Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Sui
authors.affiliation: Department of Obstetrics and gynecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of General Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Zhongchuan
authors.identifier: 0000-0002-0071-1756
authors.initials: Z
authors.lastname: Lv
mesh_terms: D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; C000608524:long non-coding RNA Linc00152, human; C000622084:long noncoding RNA CASC9, human
keywords: 
  PDAC
  ; Biomarker; CASC9; LINC00152; lncRNA