title: Green synthesis of gold nanoparticles using brown algae Cystoseira baccata: Its activity in colon cancer cells.
abstract: This study is the first dealt with the use of brown macroalgae Cystoseira baccata (CB) extracts in obtaining gold nanoparticles (Au@CB) through an eco-friendly, fast, one-pot synthetic route. The formation of spherical, stable, polycrystalline nanoparticles with mean diameter of 8.4±2.2nm was demonstrated by UV-vis spectroscopy, TEM, HRTEM, STEM and zeta potential measurements. The extract appears to act as a protective agent where the particles are embedded, keeping them separated, avoiding aggregation and coalescence. The EELS and EDS analyses confirmed the elemental composition of the extract and nanoparticles. Moreover, the functional group of biomolecules present in CB and Au@CB were characterized by FTIR. The effects of CB extract and Au@CB were tested in vitro on the colon cancer cell lines HT-29 and Caco-2, as well as on normal primary neonatal dermal fibroblast cell line PCS-201-010. Results show a stronger cytotoxic effect against HT-29 than that on Caco-2; interestingly, a lack of toxicity on PCS-201-010 was obtained. Finally, the apoptotic activity was determined; Au@CB is able to induce apoptosis activation by the extrinsic and mitochondrial pathway in our CRC in vitro model. These encouraging results suggest that Au@CB has a significant potential for the treatment of colon rectal cancer.
authors.affiliation: Departamento de Química Inorgánica, Universidade de Vigo, 36310 Vigo, Spain.
authors.forename: N
authors.initials: N
authors.lastname: González-Ballesteros
authors.affiliation: Departamento de Genética, Bioquímica e Inmunología, Universidade de Vigo, 36310 Vigo, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Prado-López
authors.affiliation: Scientific and Technological Research Assistance Center (CACTI), Universidade de Vigo, 36310 Vigo, Spain.
authors.forename: J B
authors.initials: JB
authors.lastname: Rodríguez-González
authors.affiliation: Estación de Ciencias Marinas de Toralla (ECIMAT), Universidade de Vigo, 36331 Vigo, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Lastra
authors.affiliation: Departamento de Química Inorgánica, Universidade de Vigo, 36310 Vigo, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Rodríguez-Argüelles
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D002457:Cell Extracts / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D055772:Green Chemistry Technology; D019073:HT29 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D000459:Phaeophyceae / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D002457:Cell Extracts; D006046:Gold
keywords: AuNP; Caco-2; Cystoseira baccata; Green synthesis; HT-29; TEM