title: Chemical Genetic Dissection of Membrane Trafficking.
abstract: The plant endomembrane system is an extensively connected functional unit for exchanging material between compartments. Secretory and endocytic pathways allow dynamic trafficking of proteins, lipids, and other molecules, regulating a myriad of biological processes. Chemical genetics-the use of compounds to perturb biological processes in a fast, tunable, and transient manner-provides elegant tools for investigating this system. Here, we review how chemical genetics has helped to elucidate different aspects of membrane trafficking. We discuss different strategies for uncovering the modes of action of such compounds and their use in unraveling membrane trafficking regulators. We also discuss how the bioactive chemicals that are currently used as probes to interrogate endomembrane trafficking were discovered and analyze the results regarding membrane trafficking and pathway crosstalk. The integration of different expertises and the rational implementation of chemical genetic strategies will improve the identification of molecular mechanisms that drive intracellular trafficking and our understanding of how trafficking interfaces with plant physiology and development.
authors.affiliation: Plant Molecular Biology Centre, Department of Biology, Faculty of Sciences, Universidad de Chile, 7800024 Santiago, Chile; email: lnorambuena@uchile.cl.
authors.forename: Lorena
authors.initials: L
authors.lastname: Norambuena
authors.affiliation: Plant Molecular Biology Centre, Department of Biology, Faculty of Sciences, Universidad de Chile, 7800024 Santiago, Chile; email: lnorambuena@uchile.cl.|Facultad de Recursos Naturales Renovables, Universidad Arturo Prat, 111093 Iquique, Chile.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Tejos
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D008954:Models, Biological; D010944:Plants / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport / Q000235:genetics; D013329:Structure-Activity Relationship
keywords: MoA; SAR; chemical library; endocytosis; endomembrane system; high-throughput screening; mode of action; secretory pathway; structure-activity relationship