title: Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion.
abstract: Skeletal muscle is a highly plastic tissue, which is able to regenerate after an injury. Effective and complete regeneration requires interactions between myogenic precursor cells and several cell types such as macrophages . Bone marrow derived macrophages in mouse and monocyte-derived macrophages in human are useful tools to obtain macrophage populations that may be specifically activated/polarized in vitro (e.g., pro-inflammatory, anti-inflammatory, and alternatively activated macrophages ). In vitro , human or murine primary myogenic cells recapitulate the adult myogenesis program through proliferation, myogenic differentiation, and fusion. Macrophages being highly secreting cells, they act on various biological processes including adult myogenesis . Here, we present protocols to analyze in vitro the effect of macrophage-secreted factors on muscle cell proliferation or differentiation in both mouse and human.
authors.affiliation: Department of Biosciences, University of Milan, 20133, Milan, Italy.
authors.forename: Marielle
authors.initials: M
authors.lastname: Saclier
authors.affiliation: Institut NeuroMyoGène, INMG, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5310, Villeurbanne, France.|Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Theret
authors.affiliation: Institut NeuroMyoGène, INMG, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5310, Villeurbanne, France.|Université Claude Bernard Lyon 1, Villeurbanne, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Mounier
authors.affiliation: Institut NeuroMyoGène, INMG, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5310, Villeurbanne, France. benedicte.chazaud@inserm.fr.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Chazaud
mesh_terms: D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D024510:Muscle Development / Q000187:drug effects / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned
keywords: Conditioned media; Differentiation; Human; Macrophages; Mouse; Muscle precursor cells; Myogenesis; Proliferation