title: Assessment of liver fibrosis using T1 mapping on Gd-EOB-DTPA-enhanced magnetic resonance.
abstract: BACKGROUND
  Few studies have investigated the value of Gd-EOB-DTPA-enhanced T1 mapping in exact fibrosis staging, especially its correlation with hepatic molecular transporters.
  
  
  OBJECTIVE
  To investigate the diagnostic value of Gd-EOB-DTPA-enhanced T1 mapping in staging liver fibrosis and its relationship with hepatic molecular transporters.
  
  
  METHODS
  Thirty rats were divided into the carbon tetrachloride-induced fibrosis groups and a control group. T1-mapping was performed before and 20min after administration of Gd-EOB-DTPA. The T1 relaxation time and reduction rate (Δ%) were calculated, and their correlations with the degree of fibrosis, necroinflammatory activity, iron load and hepatic molecular transporters were assessed and compared.
  
  
  RESULTS
  Hepatobiliary phase T1 relaxation time (HBP) and Δ% were different between each adjacent fibrosis subgroups(P=0.000-0.042). Very strong correlations existed between fibrosis and both HBP and Δ% (r=0.960/-0.952), and multivariate analyses revealed that fibrosis was the only factor independently predicted by HBP (P=0.000) and Δ% (P=0.001), comparing to necroinflammatory activity and iron load. The expression of the organic anion transporting polypeptide1a1 (Oatp1a1) was significantly correlated with HBP and Δ% at both mRNA (r=-0.741/0.697) and protein (r=-0.577/0.602) levels. Weaker correlations were found for multidrug resistance associated protein2 (Mrp2). Generally, both transporters showed decreasing levels with increasing degrees of fibrosis.
  
  
  CONCLUSIONS
  Gd-EOB-DTPA-enhanced T1 mapping may provide a reliable diagnostic tool in staging liver fibrosis, and can be regarded as a useful imaging biomarker of hepatocyte transporter function.
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, Xuhui District, Shanghai, China; Shanghai Institute of Medical Imaging, Xuhui District, Shanghai, China.
authors.forename: Ruo Fan
authors.initials: RF
authors.lastname: Sheng
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, Xuhui District, Shanghai, China; Shanghai Institute of Medical Imaging, Xuhui District, Shanghai, China.
authors.forename: He Qing
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, Xuhui District, Shanghai, China; Shanghai Institute of Medical Imaging, Xuhui District, Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, Xuhui District, Shanghai, China; Shanghai Institute of Medical Imaging, Xuhui District, Shanghai, China.
authors.forename: Kai Pu
authors.initials: KP
authors.lastname: Jin
authors.affiliation: Department of Pathology, Zhongshan Hospital, Fudan University, Xuhui District, Shanghai 200032, China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Xie
authors.affiliation: MR Collaboration NEA, Siemens Ltd. China, Shanghai, China.
authors.forename: Cai Xia
authors.initials: CX
authors.lastname: Fu
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, Xuhui District, Shanghai, China; Shanghai Institute of Medical Imaging, Xuhui District, Shanghai, China. Electronic address: mengsuzeng@163.com.
authors.forename: Meng Su
authors.initials: MS
authors.lastname: Zeng
mesh_terms: D000818:Animals; D027321:Anion Transport Proteins; D003287:Contrast Media; D000074062:Environmental Biomarkers; D019786:Gadolinium DTPA; D008099:Liver / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000000981:diagnostic imaging / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D029362:Organic Anion Transporters, Sodium-Independent; D021381:Protein Transport; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D027321:Anion Transport Proteins; D003287:Contrast Media; D000074062:Environmental Biomarkers; C000609070:Oatp1a1 protein, rat; D029362:Organic Anion Transporters, Sodium-Independent; C073590:gadolinium ethoxybenzyl DTPA; D019786:Gadolinium DTPA
keywords: Gadoxetic acid; Hepatic fibrosis; Magnetic resonance imaging; T1 relaxation time