title: Development of Strain-Specific Primers for Identification ofBifidobacterium bifidumBGN4.
abstract: Bifidobacterium bifidumBGN4 (BGN4) has many proven beneficial effects, including antiallergy and anticancer properties. It has been commercialized and used in several probiotic products, and thus strain-specific identification of this strain is very valuable for further strain-dependent physiological study. For this purpose, we developed novel multiplex polymerase chain reaction (PCR) primer sets for strain-specific detection of BGN4 in commercial products and fecal samples of animal models. The primer set was tested on seven strains ofB. bifidumand 75 strains of the otherBifidobacteriumspecies. The BGN4-specific regions were derived using megaBLAST against genome sequences of variousB. bifidumdatabases and four sets of primers were designed. As a result, only BGN4 produced four PCR products simultaneously whereas the other strains did not. The PCR detection limit using BGN4-specific primer sets was 2.8 × 101CFU/ml of BGN4. Those primer sets also detected and identified BGN4 in the probiotic products containing BNG4 and fecal samples from a BGN4-fed animal model with high specificity. Our results indicate that the PCR assay from this study is an efficient tool for the simple, rapid, and reliable identification of BGN4, for which probiotic strains are known.
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.|Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: So Youn
authors.initials: SY
authors.lastname: Youn
authors.affiliation: Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea.|Research Institute, Bifido Co., Ltd, Hongcheon 25117, Republic of Korea.
authors.forename: Geun Eog
authors.initials: GE
authors.lastname: Ji
authors.affiliation: Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Yoo Ri
authors.initials: YR
authors.lastname: Han
authors.affiliation: Research Institute, Bifido Co., Ltd, Hongcheon 25117, Republic of Korea.|Department of Hotel Culinary Arts, Yeonsung University, Anyang 14010, Republic of Korea.
authors.forename: Myeong Soo
authors.initials: MS
authors.lastname: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D000069985:Bifidobacterium bifidum / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial
keywords: Bifidobacterium bifidum BGN4; Multiplex PCR assay; fecal; probiotics