title: The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma.
abstract: Pseudogenes were once considered to be genomic fossils without biological function. Interestingly, recent evidence showed that a lot of pseudogenes are transcribed in human cancers, and their alterations contribute to multiple cancer development and progression. It is apparent that many pseudogenes transcribe noncoding RNAs and contribute to the role noncoding genome plays in human cancers. On this basis, some pseudogene transcripts are currently ranked among the classes of long noncoding RNAs. In this study, we identified a new pseudogene-derived long noncoding RNA termed SFTA1P by analyzing the microarray data of non-small cell lung cancer from Gene Expression Omnibus datasets. We found that SFTA1P expression was significantly decreased in non-small cell lung cancer tissues compared with normal tissues in non-small cell lung cancer microarray data. Moreover, decreased SFTA1P expression is only correlated with lung adenocarcinoma patients' poor survival time but not with lung squamous cell carcinoma patients' survival. In addition, gain-of-function studies including growth curves, migration, invasion assays, and in vivo studies were performed to verify the tumor suppressor role of SFTA1P in non-small cell lung cancer. Finally, the potential underlying pathways involved in SFTA1P were investigated by analyzing the SFTA1P-correlated genes in The Cancer Genome Atlas lung adenocarcinoma and normal tissues RNA sequencing data. Taken together, these findings demonstrate that pseudogene-derived long noncoding RNA SFTA1P exerts the tumor suppressor functions in human lung adenocarcinoma. Our investigation reveals the novel roles of pseudogene in lung adenocarcinoma, which may serve as a new target for lung adenocarcinoma diagnosis and therapy.
authors.affiliation: 1 Department of Joint Trauma, Junan County People's Hospital, Linyi, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 2 Department of Respiratory Medicine, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, People's Republic of China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: 3 Department of Clinical Laboratory, Nanjing Chest Hospital, Nanjing, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xia
authors.affiliation: 4 Department of Oncology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Wei
authors.affiliation: 5 Department of Respiratory Medicine, Huzhou Central Hospital, Huzhou, People's Republic of China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Shi
authors.affiliation: 4 Department of Oncology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Fengqi
authors.initials: F
authors.lastname: Nie
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D064593:Heterografts; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011544:Pseudogenes; D062085:RNA, Long Noncoding / Q000235:genetics*; D022661:RNA, Untranslated / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; C000616263:long non-coding RNA SFTA1P, human
keywords: Pseudogene; SFTA1P; long noncoding RNA; lung adenocarcinoma; tumor suppressor