title: Binding of Platelets to Lymphocytes: A Potential Anti-Inflammatory Therapy in Rheumatoid Arthritis.
abstract: Soluble factors released from platelets can modulate the immune response of leukocytes. We and others have recently found that T lymphocytes with bound platelets have reduced proliferation and IFN-γ and IL-17 production. Thus, we speculate that if we induce the binding of platelets to lymphocytes, we will be able to regulate the inflammatory response. When we cocultured platelets with lymphocytes at different ratios, we were able to increase the percentage of lymphocytes with bound platelets. The coculture of platelets with lymphocytes in the presence of stimulation decreased the production of IFN-γ and TNF-α, T cell proliferation, and the expression of CD25, PD-L1, and SLAM. However, this coculture increased CD39 expression. All of these effects were dependent on the dose of platelets and operated indistinctly with platelets from different healthy donors. When platelets were cocultured in the same compartment with lymphocytes, we observed less IFN-γ and TNF-α production and T lymphocyte proliferation than in cultures with platelets separated from lymphocytes by a 0.4-μm pore size filter. The binding of platelets to lymphocytes was blocked with anti-P-selectin Abs, and when this occurred we observed higher IFN-γ and TNF-α production than in nonblocked conditions. The cocultures of platelets with synovial fluid cells from rheumatoid arthritis patients reduced inflammatory cytokine production and increased IL-10 production. These results suggest that platelet binding to lymphocytes effectively regulates T lymphocyte function. This mechanism could be easily applied to reduce inflammatory responses.
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain; czamora@santpau.cat svidal@santpau.cat.
authors.forename: Carlos
authors.initials: C
authors.lastname: Zamora
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Cantó
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Nieto
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Bardina
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.|Unitat Reumatologia, Hospital de la Santa Creu i Sant Pau, 08026 Barcelona, Spain; and.
authors.forename: Cesar
authors.initials: C
authors.lastname: Diaz-Torné
authors.affiliation: Unitat Reumatologia, Hospital de la Santa Creu i Sant Pau, 08026 Barcelona, Spain; and.
authors.forename: Patricia
authors.initials: P
authors.lastname: Moya
authors.affiliation: Unitat Reumatologia, Hospital de la Santa Creu i Sant Pau, 08026 Barcelona, Spain; and.
authors.forename: Berta
authors.initials: B
authors.lastname: Magallares
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.
authors.forename: M Angels
authors.initials: MA
authors.lastname: Ortiz
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.
authors.forename: Germà
authors.initials: G
authors.lastname: Julià
authors.affiliation: Departament Immunologia, Hospital de la Santa Creu i Sant Pau, 08026 Barcelona, Spain.
authors.forename: Candido
authors.identifier: 0000-0003-2235-9893
authors.initials: C
authors.lastname: Juarez
authors.affiliation: Unitat Reumatologia, Hospital de la Santa Creu i Sant Pau, 08026 Barcelona, Spain; and.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Llobet
authors.affiliation: Departament Immunologia, Institut Recerca Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain; czamora@santpau.cat svidal@santpau.cat.
authors.forename: Silvia
authors.initials: S
authors.lastname: Vidal
mesh_terms: D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016207:Cytokines