title: Isolation of a new resorcinolic lipid from Mangifera zeylanica Hook.f. bark and its cytotoxic and apoptotic potential.
abstract: Mangifera zeylanica is a plant endemic to Sri Lanka and its bark has been used in traditional medicine to treat some cancers. This study was aimed to isolate potentially cytotoxic compound/s from the hexane extract of the bark of M. zeylanica by bio-activity guided fractionation. The structure of the isolated compound (1) was elucidated using1H,13C NMR and mass spectrometric techniques. Compound 1 was identified as a new resorcinolic lipid (5-((8Z, 11Z, 14Z)-hexatriaconta-8, 11, 14-trienyl) benzene-1,3-diol). Apoptotic potential of the isolated compound was determined only in MCF-7 (estrogen receptor positive) breast cancer cells to which it was more cytotoxic than to normal mammary epithelial cells. Oxidative stress markers [reactive oxygen species (ROS), glutathione levels (GSH) and glutathione-S-transferase (GSH)] were also determined in MCF-7 cells treated with compound 1. Treatment with compound 1 led to an increase in caspase 7 activity, morphological features of apoptosis and DNA fragmentation in MCF-7 cells. Furthermore, it also led to an increase in ROS and GST levels while depleting GSH levels. Results of this study suggest that isolated new resorcinolic lipid can induce apoptosis in MCF-7 cells, possibly via oxidative stress mechanism.
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, 90, Cumaratunga Munidasa Mawatha, Colombo 03, Sri Lanka. Electronic address: mk.ediriweera@gmail.com.
authors.forename: Meran Keshawa
authors.initials: MK
authors.lastname: Ediriweera
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, 90, Cumaratunga Munidasa Mawatha, Colombo 03, Sri Lanka. Electronic address: kamani@ibmbb.cmb.ac.lk.
authors.forename: Kamani Hemamala
authors.initials: KH
authors.lastname: Tennekoon
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, 90, Cumaratunga Munidasa Mawatha, Colombo 03, Sri Lanka. Electronic address: sam@ibmbb.cmb.ac.lk.
authors.forename: Sameera Ranganath
authors.initials: SR
authors.lastname: Samarakoon
authors.affiliation: HEJ Research Institute of Chemistry, University of Karachi, Pakistan. Electronic address: adhikarimine@yahoo.com.
authors.forename: Achyut
authors.initials: A
authors.lastname: Adhikari
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, 90, Cumaratunga Munidasa Mawatha, Colombo 03, Sri Lanka. Electronic address: irathab@gmail.com.
authors.forename: Ira
authors.initials: I
authors.lastname: Thabrew
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, 90, Cumaratunga Munidasa Mawatha, Colombo 03, Sri Lanka. Electronic address: edilip.desilva@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Dilip de Silva
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism; D053179:Caspase 7; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000494:pharmacology; D061986:MCF-7 Cells; D031022:Mangifera / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012118:Resorcinols / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008055:Lipids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012118:Resorcinols; D005982:Glutathione Transferase; D053179:Caspase 7; D005978:Glutathione
keywords: Anti cancer activity; Apoptosis; Cytotoxicity; Mangifera zeylanica; Oxidative stress; Resorcinolic lipid