title: [RNA interference of HERC4 inhibits proliferation, apoptosis and migration of cervical cancer Hela cells].
abstract: OBJECTIVE
  To explore the effects of silencing HERC4 on the proliferation, apoptosis, and migration of cervical cancer cell line Hela and the possible molecular mechanisms.
  
  
  METHODS
  Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells, and HERC4 expression in the cells was examined with Western blotting. CCK-8 assay, annexin V-FITC/PI assay, and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation, apoptosis and migration ability of Hela cells. The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.
  
  
  RESULTS
  Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P<0.01). HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells, increased the apoptosis rate (P<0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P<0.01).
  
  
  CONCLUSIONS
  Silencing of HERC4 efficiently inhibits the proliferation, migration, and invasion of Hela cells in vitro, and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.
authors.affiliation: Clinical Laboratory, Nanshan Maternity Child Healthcare Hospital, Shenzhen 518067, China. E-mail: weimin78@126.com.
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Cui-Xia
authors.initials: CX
authors.lastname: Cai
authors.forename: Han-Duo
authors.initials: HD
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006367:HeLa Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000473:pathology
chemical_list: C000595707:BCL2 protein, human; C530104:CCND1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; D019938:Cyclin D1; C502268:HERC4 protein, human; D044767:Ubiquitin-Protein Ligases