title: A deletion hampering appropriate typing of Mycobacterium africanum.
abstract: Molecular epidemiology analysis of tuberculosis transmission is based mostly on the application of MIRU-VNTR. In certain isolates a complete 24-loci genotype is not obtained and these incompletely genotyped isolates can not be used in the definition of clusters. In a population-based molecular epidemiology study performed in Almería, Southeast Spain, a context with a high proportion of immigrants, we found that an 88-bp deletion in isolates of Mycobacterium africanum Lineage 5 hampers MIRU-VNTR analysis. A more extensive analysis revealed that this deletion was shared by all the Lineage 5 isolates in certain countries of origin of immigrants, such as Equatorial Guinea, and is likely present in several other African countries and also in the USA. A procedure is proposed to enable epidemiological analysis of these isolates.
authors.affiliation: Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Abascal
authors.affiliation: Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Grupo de Investigación en Sanidad Animal y Humana, Departamento de Ciencias de la Vida y la Agricultura Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador.
authors.forename: Diana Maricela
authors.initials: DM
authors.lastname: Herrera
authors.affiliation: Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Herranz
authors.affiliation: Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Sheila
authors.initials: S
authors.lastname: Santantón
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Torrecárdenas, Almería, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Martínez-Lirola
authors.affiliation: Servei de Microbiologia, Hospital Clinic-CDB, Barcelona, Spain; IS Global, Barcelona, Spain.
authors.forename: Griselda
authors.initials: G
authors.lastname: Tudó
authors.affiliation: Servei de Microbiologia, Hospital Clinic-CDB, Barcelona, Spain; IS Global, Barcelona, Spain.
authors.forename: Juliá
authors.initials: J
authors.lastname: Gonzalez
authors.affiliation: Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.affiliation: Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain. Electronic address: lperezg00@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez-Lago
authors.affiliation: Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain. Electronic address: dgviedma2@gmail.com.
authors.forename: Darío
authors.initials: D
authors.lastname: García-de-Viedma
mesh_terms: D000349:Africa / Q000208:ethnology; D015373:Bacterial Typing Techniques; D044383:Black People; D004269:DNA, Bacterial / Q000235:genetics*; D054242:Emigrants and Immigrants; D004641:Emigration and Immigration; D017353:Gene Deletion; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D020071:Interspersed Repetitive Sequences; D018598:Minisatellite Repeats; D025202:Molecular Diagnostic Techniques; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013030:Spain / Q000453:epidemiology; D014376:Tuberculosis / Q000175:diagnosis / Q000208:ethnology / Q000382:microbiology* / Q000635:transmission
chemical_list: D004269:DNA, Bacterial
keywords: DNA deletion; Genotyping; MIRU-VNTR; Mycobacterium africanum