title: Mitochondrial ABC Transporter ATM3 Is Essential for Cytosolic Iron-Sulfur Cluster Assembly.
abstract: The mitochondrial ATP-binding cassette transporter ATM3 has been studied in Arabidopsis. Its function, however, is poorly understood in other model plant species. This study reports that the ATM3 is required for cytosolic iron-sulfur cluster assembly and is essential for meristem maintenance in rice (Oryza sativa). The loss of function ofOsATM3is lethal in rice at the four-leaf stage. In theosatm3T-DNA insertion mutant, the fourth leaf fails to develop and the lateral roots are short. Cytosolic iron-sulfur protein activities were significantly reduced in bothosatm3and RNA interference transgenic lines. The expression profiles of many iron metabolism genes were altered in theosatm3and RNA interference lines. Glutathione metabolism was impaired and reactive oxygen species, particularly superoxide, accumulated inosatm3Promoter-β-glucuronidase staining of the transgenic line indicated thatOsATM3is highly expressed in lateral root primordia, root tip meristem zones, and shoot apical meristem regions. The average cell size was significantly greater inosatm3than in the wild type. Massive cell death occurred in theosatm3root tip meristem zone. Quantitative RT-PCR revealed transcriptional reprogramming of the genes in theosatm3and RNAi lines involved in DNA repair and cell cycle arrest. Our results suggest that the mitochondrial ATM3 is essential for iron homeostasis in rice.
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (J.Z., Z.W., Y.L., Z.S., X.F., M.Z., H.Y.); and.|University of the Chinese Academy of Sciences, Beijing 100049, China (J.Z., Z.W., Y.L.).
authors.forename: Jia
authors.identifier: 0000-0002-5350-2456
authors.initials: J
authors.lastname: Zuo
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (J.Z., Z.W., Y.L., Z.S., X.F., M.Z., H.Y.); and.|University of the Chinese Academy of Sciences, Beijing 100049, China (J.Z., Z.W., Y.L.).
authors.forename: Zhigeng
authors.identifier: 0000-0001-7026-6240
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (J.Z., Z.W., Y.L., Z.S., X.F., M.Z., H.Y.); and.|University of the Chinese Academy of Sciences, Beijing 100049, China (J.Z., Z.W., Y.L.).
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (J.Z., Z.W., Y.L., Z.S., X.F., M.Z., H.Y.); and.|University of the Chinese Academy of Sciences, Beijing 100049, China (J.Z., Z.W., Y.L.).
authors.forename: Zedan
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (J.Z., Z.W., Y.L., Z.S., X.F., M.Z., H.Y.); and.|University of the Chinese Academy of Sciences, Beijing 100049, China (J.Z., Z.W., Y.L.).
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Feng
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (J.Z., Z.W., Y.L., Z.S., X.F., M.Z., H.Y.); and.|University of the Chinese Academy of Sciences, Beijing 100049, China (J.Z., Z.W., Y.L.).
authors.forename: Mingyong
authors.identifier: 0000-0001-5613-6458
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (J.Z., Z.W., Y.L., Z.S., X.F., M.Z., H.Y.); and yejhong@gmail.com.|University of the Chinese Academy of Sciences, Beijing 100049, China (J.Z., Z.W., Y.L.) yejhong@gmail.com.
authors.forename: Hong
authors.identifier: 0000-0003-3360-994X
authors.initials: H
authors.lastname: Ye
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D059447:Cell Cycle Checkpoints / Q000235:genetics; D003600:Cytosol / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D020043:Genes, Essential / Q000235:genetics; D005804:Genes, Lethal; D005978:Glutathione / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000378:metabolism; D018519:Meristem / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013481:Superoxides / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides; D005978:Glutathione