title: Safety of Using Matrix Metalloproteinase Inhibitor in Experimental Glaucoma Filtration Surgery.
abstract: We evaluated the safety of matrix metalloproteinase (MMP) inhibitor in experimental glaucoma filtration surgery in an animal model. Fifteen New Zealand white rabbits underwent an experimental trabeculectomy and were randomly allocated into 3 groups according to the adjuvant agent: no treatment group (n = 5), 0.02% mitomycin C (MMC) soaking group (n = 5), and MMP inhibitor (ilomastat) subconjunctival injection group (n = 5). Slit lamp examination with Seidel testing, pachymetry, and specular microscopy was performed preoperatively and postoperatively. The conjunctiva and ciliary body toxicity were evaluated with scores according to the pathologic grading systems. Electron microscopy was used to examine the structural changes in cornea, conjunctiva, and ciliary body. In the ilomastat-treated group, there was no statistically significant change in central corneal thickness preoperatively and at 28 days postoperatively (P = 0.655). There were also no significant changes in specular microscopy findings over the duration of the study in the ilomastat-treated group. The conjunctival toxicity score was 1 in the control group, 1.5 in the ilomastat-treated group, and 2 in the MMC-treated group. When assessing ciliary body toxicity scores, the ilomastat-treated group score was 0.5 and the MMC-treated group score was 1.5. Transmission electron microscopy did not show structural changes in the cornea and ciliary body whereas the structural changes were noticed in MMC group. A single subconjunctival injection of MMP inhibitor during the experimental trabeculectomy showed a less toxic affect in the rabbit cornea, conjunctiva, and ciliary body compared to MMC.
authors.affiliation: Department of Ophthalmology, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea.
authors.forename: Wool
authors.identifier: http://orcid.org/0000-0002-1953-5684
authors.initials: W
authors.lastname: Suh
authors.affiliation: Department of Ophthalmology, Institute of Ophthalmology and Optometry, Ewha Womans University Mok-Dong Hospital, Ewha Womans University School of Medicine, Seoul, Korea.
authors.forename: Kyung Eun
authors.identifier: http://orcid.org/0000-0001-8484-6086
authors.initials: KE
authors.lastname: Han
authors.affiliation: Department of Ophthalmology, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea. scarpel@hally.or.kr.
authors.forename: Jae Ryong
authors.identifier: http://orcid.org/0000-0003-1663-1440
authors.initials: JR
authors.lastname: Han
mesh_terms: D000818:Animals; D002924:Ciliary Body / Q000187:drug effects / Q000502:physiology; D003228:Conjunctiva / Q000187:drug effects* / Q000502:physiology; D003315:Cornea / Q000187:drug effects / Q000502:physiology; D005901:Glaucoma / Q000601:surgery*; D006877:Hydroxamic Acids; D007211:Indoles / Q000633:toxicity*; D061965:Matrix Metalloproteinase Inhibitors / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission; D016685:Mitomycin / Q000633:toxicity; D011817:Rabbits; D014130:Trabeculectomy
chemical_list: D006877:Hydroxamic Acids; D007211:Indoles; D061965:Matrix Metalloproteinase Inhibitors; D016685:Mitomycin; C119203:ilomastat
keywords: Filtration; Glaucoma; Matrix Metalloproteinase Inhibitor