title: Accelerated Combinatorial High Throughput Star Polymer Synthesis via a Rapid One-Pot Sequential Aqueous RAFT (rosa-RAFT) Polymerization Scheme.
abstract: Advanced polymerization methodologies, such as reversible addition-fragmentation transfer (RAFT), allow unprecedented control over star polymer composition, topology, and functionality. However, using RAFT to produce high throughput (HTP) combinatorial star polymer libraries remains, to date, impracticable due to several technical limitations. Herein, the methodology "rapid one-pot sequential aqueous RAFT" or "rosa-RAFT," in which well-defined homo-, copolymer, and mikto-arm star polymers can be prepared in very low to medium reaction volumes (50 µL to 2 mL) via an "arm-first" approach in air within minutes, is reported. Due to the high conversion of a variety of acrylamide/acrylate monomers achieved during each successive short reaction step (each taking 3 min), the requirement for intermediary purification is avoided, drastically facilitating and accelerating the star synthesis process. The presented methodology enables RAFT to be applied to HTP polymeric bio/nanomaterials discovery pipelines, in which hundreds of complex polymeric formulations can be rapidly produced, screened, and scaled up for assessment in a wide range of applications.
authors.affiliation: Australian Institute of Bioengineering and Nanotechnology (AIBN), University of Queensland, 4072, St Lucia, QLD, Australia.|Manufacturing, Commonwealth Scientific and Industrial Research Organization (CSIRO), 3168, Clayton, VIC, Australia.
authors.forename: Steffen
authors.initials: S
authors.lastname: Cosson
authors.affiliation: Manufacturing, Commonwealth Scientific and Industrial Research Organization (CSIRO), 3168, Clayton, VIC, Australia.
authors.forename: Maarten
authors.initials: M
authors.lastname: Danial
authors.affiliation: Manufacturing, Commonwealth Scientific and Industrial Research Organization (CSIRO), 3168, Clayton, VIC, Australia.
authors.forename: Julien Rosselgong
authors.initials: JR
authors.lastname: Saint-Amans
authors.affiliation: Australian Institute of Bioengineering and Nanotechnology (AIBN), University of Queensland, 4072, St Lucia, QLD, Australia.|Manufacturing, Commonwealth Scientific and Industrial Research Organization (CSIRO), 3168, Clayton, VIC, Australia.|School of Chemical Engineering, University of Queensland, 4072, St Lucia, QLD, Australia.
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Cooper-White
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D008956:Models, Chemical; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D011108:Polymers; D020106:Acrylamide; C036658:acrylic acid
keywords: high throughput screening; mikto-arm star polymer; reversible addition-fragmentation chain transfer (RAFT)