title: A new putative alphapartitivirus recovered from the powdery mildew fungus Erysiphe palczewskii.
abstract: Two double-stranded RNAs (dsRNA) likely representing the genome of a novel alphapartitivirus which we provisionally named Erysiphe palczewskii alphapartitivirus 1 (EpV1) were recovered from the powdery mildew fungus E. palczewskii infecting Sophora japonica in Jingzhou, Hubei province of China. The two dsRNAs, 1955 (dsRNA1) and 1917 (dsRNA2) bp in size, respectively, each contains a single open reading frame (ORF) encoding a 585- and 528-aa protein, respectively. The 585-aa protein contains a conserved RNA-dependent RNA polymerase (RdRp) domain and shows significant homology to RdRps of approved or putative partitiviruses, particularly those belonging to the genus Alphapartitivirus. However, it shares an aa sequence identity lower than 80% with its closest relative, the RdRp of the putative alphapartitivirus Grapevine partitivirus, and lower than 60% with the RdRps of other partitiviruses. In a phylogenetic tree constructed with RdRp aa sequences of selected partitiviruses, the putative virus EpV1 clustered with Grapevine partitivirus and formed a well-supported monophyletic clade with known or putative alphapartitiviruses.
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crop, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Guihong
authors.initials: G
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crop, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Qiu
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, Guizhou, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crop, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Saif Ul
authors.initials: SU
authors.lastname: Islam
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Shouguo
authors.initials: S
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crop, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crop, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. yangtze2008@126.com.|Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China. yangtze2008@126.com.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crop, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. duzhenguo1228@163.com.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
mesh_terms: D001203:Ascomycota / Q000821:virology*; D002681:China; D016679:Genome, Viral; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics*; D012330:RNA, Double-Stranded; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017423:Sequence Analysis, RNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase
keywords: Erysiphe palczewskii; Partitivirus; Powdery mildew; dsRNA