title: [Preparation of Platelet-Rich Plasma from the White Slurry and Its Effect on MSC Proliferation].
abstract: OBJECTIVE
  To isolate platelet-rich plasma(PRP) from the white slurry(WS), a depleted fraction of the clinical blood supply, so as to provide an easier method to harvest PRP for related studies and clinical use.
  
  
  METHODS
  The protocols preparing PRP from whole blood and WS were compared. The morphological characteristics of the different PRPs were observed under transmission electron microscope; the expression of the platelet markers CD41a and CD42b were detected by the flow cytometry. Moreover, the ingredients of the PRPs were measured by using cytoanalyzer. for detecting the physiological function of the PRP, the harvested PRP were added to MSC culture and the cell proliferation was detected by using CCK-8 method.
  
  
  RESULTS
  a large amount of PRP from WS was easier harvested. the WS-derived PRP shared similar morphological characteristics and ingredients as compared with whole blood-derived PRP. Importantly, the WS-derived PRP exhibited a higher expression of CD41a and CD42b than that of traditional PRP, which indicate that the WS is a promising reservoir for PRP.
  
  
  CONCLUSIONS
  The WS can be used to prepare PRP, and the novel PRP share similar biological characteristics as traditional PRP prepared from whole blood. The present study provides an easier and economical method to harvest PRP and this findings may be helpful for PRP related studies.
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ke-Tao
authors.initials: KT
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China. E-mail: lizhongli@263.net.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Li
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. E-mail:zhudingdingabc@163.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Qin
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yi-Meng
authors.initials: YM
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ru-Ju
authors.initials: RJ
authors.lastname: Lv
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Mao
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001792:Blood Platelets; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D053657:Platelet-Rich Plasma; D013234:Stem Cells