title: Mandevilla longiflora (Desf.) Pichon improves airway inflammation in a murine model of allergic asthma.
abstract: BACKGROUND
  Mandevilla longiflora, popularly known as "velame" in central Brazil, is a subshrub widely distributed in South America. Its xylopodium is used in the form of a decoction or infusion to treat inflammation and other ailments.
  
  
  OBJECTIVE
  This study aimed to evaluate the anti-inflammatory potential of M. longiflora in an in vivo model of ovalbumin-induced immediate hypersensitivity, identifying its effects on leukocyte infiltration, IgE and LTB4levels, and Th2 cytokine production. In addition, HPLC fingerprint of the extract was performed.
  
  
  METHODS
  The hydroethanolic extract 70% of M. longiflora (HEMI) was obtained by maceration of the plant xylopodium. Swiss mice were sensitized by i.p. injection OVA-aluminium hydroxide on days 1 and 10. Nine days after the last sensitisation animals were challenged for 6 consecutive days with OVA solution for 20min daily in a closed chamber under continuous flow of aerosol. The animals were treated with HEMl (20, 50 and 200mg/kg p.o.), 2 times per day, and euthanized 24h later. Animals treated with vehicle (2% Tween-20) or dexamethasone were used as negative and positive controls, respectively. The recruitment of inflammatory cells into the pulmonary cavity was evaluated by counting cells present in broncho-alveolar lavage fluid (BALF). Lung tissue was also collected for histopathology and infiltration analysis. Quantification of IL-4, IL-5 and IL-13 from the BALF, and IgE, and LTB4from plasma, were conducted by immunoassay.
  
  
  CONCLUSIONS
  The HEMl attenuated leukocyte migration into the airways, which was evidenced by a decrease in eosinophils, neutrophils and mononuclear cells, both in BALF quantification and by histopathological analysis, as well as decreasing the concentrations of IL-4, IL-5, IL-13, IgE and LTB4. All of these events are typical of air-mucosa inflammatory disease. These findings scientifically evidence for the first time the ethnopharmacological use of M. longiflora to treat chronic inflammatory events, such as asthma, and suggest a potential therapeutic use or complementary therapy for this plant extract.
authors.affiliation: Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil.
authors.forename: Danielle Ayr Tavares
authors.initials: DA
authors.lastname: de Almeida
authors.affiliation: Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil.
authors.forename: Suellen Iara Guirra
authors.initials: SI
authors.lastname: Rosa
authors.affiliation: Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil.
authors.forename: Thais Campos Dias
authors.initials: TC
authors.lastname: da Cruz
authors.affiliation: Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Pavan
authors.affiliation: Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil.
authors.forename: Amílcar
authors.initials: A
authors.lastname: Sabino Damazo
authors.affiliation: Research Laboratory of Natural Products (LPPN), Federal University of Tocantins, Av. NS15, Palmas, Tocantins 77020-210, Brazil.
authors.forename: Ilsamar Mendes
authors.initials: IM
authors.lastname: Soares
authors.affiliation: Research Laboratory of Natural Products (LPPN), Federal University of Tocantins, Av. NS15, Palmas, Tocantins 77020-210, Brazil.
authors.forename: Sérgio Donizeti
authors.initials: SD
authors.lastname: Ascêncio
authors.affiliation: Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Macho
authors.affiliation: Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil. Electronic address: taba@terra.com.br.
authors.forename: Domingos Tabajara de Oliveira
authors.initials: DT
authors.lastname: Martins
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D029062:Apocynaceae; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000633:toxicity; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D010047:Ovalbumin
keywords: Asthma; Inflammation; Mandevilla longiflora