title: Label-free detection of hepatitis B virus using solution immersed silicon sensors.
abstract: Highly sensitive solution immersed silicon (SIS) biosensors were developed for detection of hepatitis B virus (HBV) infection in the early stage. The ultrasensitivity for overlayer thickness at the nonreflecting condition for the p-polarized wave is the basis of SIS sensing technology. The change in thickness due to biomolecular interactions and change in refractive index of the surrounding buffer medium were assessed simultaneously using two separate ellipsometric parameters (Ψ and Δ), respectively, from a single sensing spot. A direct antigen-antibody affinity assay was used to detect and quantify hepatitis B surface antigen (HBsAg), which is the early stage biomarker for HBV infection. The detection limit of 10 pg/ml was achieved for HBsAg in the human blood serum, which is comparable with the results of enzyme-linked immunosorbent assay and other hybrid assays. The SIS sensor's response time was less than 10 min. The SIS sensors exhibit excellent stability and high signal-to-noise ratio, and are cost-effective, which makes them a suitable candidate for point-of-care applications.
authors.affiliation: Center for Nanometrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea.
authors.forename: Mangesh S
authors.initials: MS
authors.lastname: Diware
authors.affiliation: Center for Nanometrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea.
authors.forename: Hyun Mo
authors.initials: HM
authors.lastname: Cho
authors.affiliation: Center for Nanometrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea.
authors.forename: Won
authors.initials: W
authors.lastname: Chegal
authors.affiliation: Center for Nanometrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea.
authors.forename: Yong Jai
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Department of Bio-Microsystem Technology, Korea University, Seoul 02841, South Korea.
authors.forename: Sang Won
authors.initials: SW
authors.lastname: O
authors.affiliation: Department of Bio-Microsystem Technology, Korea University, Seoul 02841, South Korea.
authors.forename: Se-Hwan
authors.initials: SH
authors.lastname: Paek
authors.affiliation: Department of Mechanical System Engineering, Chosun University, Gwangju 61452, South Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Department of Mechanical System Engineering, Chosun University, Gwangju 61452, South Korea.
authors.forename: Kyeong-Suk
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Photonics and Sensors, Hannam University, Daejeon 306791, South Korea.
authors.forename: Yoon Gi
authors.initials: YG
authors.lastname: Min
authors.affiliation: Department of Photonics and Sensors, Hannam University, Daejeon 306791, South Korea.
authors.forename: Jae Heung
authors.initials: JH
authors.lastname: Jo
authors.affiliation: Center for Nanometrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea.
authors.forename: ChaeHo
authors.initials: C
authors.lastname: Shin
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003362:Cost-Benefit Analysis; D006509:Hepatitis B / Q000175:diagnosis*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D019095:Point-of-Care Systems; D012825:Silicon / Q000378:metabolism; D013997:Time Factors
chemical_list: D006514:Hepatitis B Surface Antigens; D012825:Silicon