title: Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita.
abstract: The full cDNA of Mi-ace-3 encoding an acetylcholinesterase (AChE) in Meloidogyne incognita was cloned and characterized. Mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. Key residues essential to AChE structure and function were conserved. The deduced Mi-ACE-3 protein sequence had 72% amino acid similarity with that of Ditylenchus destructor Dd-AChE-3. Phylogenetic analyses using 41 AChEs from 24 species showed that Mi-ACE-3 formed a cluster with 4 other nematode AChEs. Our results revealed that the Mi-ace-3 cloned in this study, which is orthologous to Caenorhabditis elegans AChE, belongs to the nematode ACE-3/4 subgroup. There was a significant reduction in the number of galls in transgenic tobacco roots when Mi-ace-1, Mi-ace-2, and Mi-ace-3 were knocked down simultaneously, whereas little or no effect were observed when only one or two of these genes were knocked down. This is an indication that the functions of these three genes are redundant.
authors.affiliation: College of Agronomy/ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, Jiangxi, China. Electronic address: cuiruqiang@jxau.edu.cn.
authors.forename: Ruqiang
authors.initials: R
authors.lastname: Cui
authors.affiliation: College of Agronomy/ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Agronomy/ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Wenkun
authors.initials: W
authors.lastname: Huang
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Chengming
authors.initials: C
authors.lastname: Fan
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Peng
authors.affiliation: School of Integrative Plant Science, Plant Pathology and Plant-Microbe Biology Section, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Washington
authors.initials: W
authors.lastname: da Silva
authors.affiliation: College of Agronomy/ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, Jiangxi, China. Electronic address: xtsun80@163.com.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Sun
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D015971:Gene Expression Regulation, Enzymologic; D055785:Gene Knockdown Techniques; D018551:Solanum lycopersicum / Q000469:parasitology; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames; D010802:Phylogeny; D018517:Plant Roots / Q000469:parasitology; D010941:Plant Tumors / Q000235:genetics / Q000469:parasitology; D034622:RNA Interference / Q000502:physiology; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000469:parasitology; D014431:Tylenchoidea / Q000145:classification / Q000201:enzymology / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Expression; Meloidogyne incognita; Phylogenetic analysis; RNAi