title: Silencing of Two Insulin Receptor Genes Disrupts Nymph-Adult Transition of Alate Brown Citrus Aphid.
abstract: Insulin receptors play key roles in growth, development, and polymorphism in insects. Here, we report two insulin receptor genes (AcInR1andAcInR2) from the brown citrus aphid,Aphis(Toxoptera)citricidus. Transcriptional analyses showed thatAcInR1increased during the nymph-adult transition in alate aphids, whileAcInR2had the highest expression level in second instar nymphs.AcInR1is important in aphid development from fourth instar nymphs to adults as verified by dsRNA feeding mediated RNAi. The silencing ofAcInR1or/andAcInR2produced a variety of phenotypes including adults with normal wings, malformed wings, under-developed wings, and aphids failing to develop beyond the nymphal stages. Silencing ofAcInR1orAcInR2alone, and co-silencing of both genes, resulted in 73% or 60%, and 87% of aphids with problems in the transition from nymph to normal adult. The co-silencing ofAcInR1andAcInR2resulted in 62% dead nymphs, but no mortality occurred by silencing ofAcInR1orAcInR2alone. Phenotypes of adults in the dsInR1and dsInR2were similar. The results demonstrate thatAcInR1andAcInR2are essential for successful nymph-adult transition in alate aphids and show that RNAi methods may be useful for the management of this pest.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. biyueding@163.com.
authors.forename: Bi-Yue
authors.initials: BY
authors.lastname: Ding
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. fengshang1994@yahoo.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Shang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. qiangzhang1996@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. yingxiong333@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. qunyang1023@163.com.
authors.forename: Qun
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. jinzhiniu@swu.edu.cn.
authors.forename: Jin-Zhi
authors.initials: JZ
authors.lastname: Niu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. guy.smagghe@ugent.be.|Department of Crop Protection, Ghent University, 9000 Ghent, Belgium. guy.smagghe@ugent.be.
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. wangjinjun@swu.edu.cn.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001042:Aphids / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D020868:Gene Silencing; D017344:Genes, Insect; D008018:Life Cycle Stages; D009758:Nymph / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D034622:RNA Interference; D011972:Receptor, Insulin / Q000235:genetics*
chemical_list: D011972:Receptor, Insulin
keywords: Aphis (Toxoptera) citricidus; RNAi; development; insulin receptor genes