title: Protein Profiling Reveals Novel Proteins in Pollen and Pistil of W22 (ga1; Ga1) in Maize.
abstract: Gametophytic factors mediate pollen-pistil interactions in maize (Zea maysL.) and play active roles in limiting gene flow among maize populations and between maize and teosinte. This study was carried out to identify proteins and investigate the mechanism of gametophytic factors using protein analysis. W22 (ga1); which did not carry a gametophytic factor and W22 (Ga1), a near iso-genic line, were used for the proteome investigation. SDS-PAGE was executed to investigate proteins in the pollen and pistil of W22 (ga1) and W22 (Ga1). A total of 44 differentially expressed proteins were identified in the pollen and pistil on SDS-PAGE using LTQ-FTICR MS. Among the 44 proteins, a total of 24 proteins were identified in the pollen of W22 (ga1) and W22 (Ga1) whereas 20 differentially expressed proteins were identified from the pistil of W22 (ga1) and W22 (Ga1). However, in pollen, 2 proteins were identified only in the W22 (ga1) and 12 proteins only in the W22 (Ga1) whereas 10 proteins were confirmed from the both of W22 (ga1) and W22 (Ga1). In contrary, 10 proteins were appeared only in the pistil of W22 (ga1) and 7 proteins from W22 (Ga1) while 3 proteins confirmed in the both of W22 (ga1) and W22 (Ga1). Moreover, the identified proteins were generally involved in hydrolase activity, nucleic acid binding and nucleotide binding. These results help to reveal the mechanism of gametophytic factors and provide a valuable clue for the pollen and pistil research in maize.
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea. edjlsa8603@naver.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea. swapankhulna@gmail.com.
authors.forename: Swapan Kumar
authors.initials: SK
authors.lastname: Roy
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea. abuhena7@gmail.com.
authors.forename: Abu Hena Mostafa
authors.initials: AH
authors.lastname: Kamal
authors.affiliation: Division of Mass Spectrometry Research, Korea Basic Science Institute, Chungbuk 863-883, Korea. chokun@kbsi.re.kr.
authors.forename: Kun
authors.initials: K
authors.lastname: Cho
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea. kwonsj1220@naver.com.
authors.forename: Soo-Jeong
authors.initials: SJ
authors.lastname: Kwon
authors.affiliation: Lab of Molecular Breeding, Arid land Research Center, Tottori University, Tottori 680-8550, Japan. whtjddn0435@chungbuk.ac.kr.
authors.forename: Seong-Woo
authors.initials: SW
authors.lastname: Cho
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea. yoonsupso@cbnu.ac.kr.
authors.forename: Yoon-Sup
authors.initials: YS
authors.lastname: So
authors.affiliation: USDA-ARS Plant Science Research Unit, Department of Crop Science, Box 7620, North Carolina State University, Raleigh, NC 27695, USA. Jim.Holland@ars.usda.gov.
authors.forename: James B
authors.initials: JB
authors.lastname: Holland
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea. shwoo@chungbuk.ac.kr.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Woo
keywords: gametophytic factors; maize; pistil; pollen; proteomics