title: Facile fabrication of polyurethane microcapsules carriers for tracing cellular internalization and intracellular pH-triggered drug release.
abstract: A tailor-made traceable pH-sensitive drug delivery system based on polyurethane (PU) microcapsules was fabricated using a facile double-emulsion method containing 3,3'-dioctadecyloxacarbocyanine perchlorate, doxorubicin (DOX) and sodium bicarbonate (NaHCO3). When PU microcapsules were immersed in acidic media, NaHCO3could react with the H+to quickly produce CO2bubbles to puncture the PU shell, resulting in rapid release of DOX to promptly reach the intracellular drug therapeutic threshold to kill cancer cells in a short period. Confocal laser scanning microscopic analysis showed that these traceable pH-sensitive drug carriers can be easily internalized by BGC 823 and Hela cells, and the loaded DOX can quickly release from PU microcapsules in the endo-/lysosomes to be mainly resided in cell nuclei. This traceable pH-sensitive drug carrier can achieve on-demand controlled release profiles for visualization of cancer therapy. Thus, it is a potential candidate for anticancer drug delivery system in advanced cancer therapy.
authors.affiliation: Nanshan District Key Laboratory for Biopolymers and Safety Evaluation, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Polymer Science and Technology, Shenzhen 518060, PR China; Guangdong Research Center for Interfacial Engineering of Functional Materials, Shenzhen 518060, PR China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen University Health Science Center, Shenzhen 518060, PR China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Nanshan District Key Laboratory for Biopolymers and Safety Evaluation, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Polymer Science and Technology, Shenzhen 518060, PR China; Guangdong Research Center for Interfacial Engineering of Functional Materials, Shenzhen 518060, PR China.
authors.forename: Florian J
authors.initials: FJ
authors.lastname: Stadler
authors.affiliation: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.affiliation: Shenzhen University Health Science Center, Shenzhen 518060, PR China.
authors.forename: Siping
authors.initials: S
authors.lastname: Chen
authors.affiliation: Nanshan District Key Laboratory for Biopolymers and Safety Evaluation, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Polymer Science and Technology, Shenzhen 518060, PR China; Guangdong Research Center for Interfacial Engineering of Functional Materials, Shenzhen 518060, PR China. Electronic address: csg@szu.edu.cn.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D002214:Capsules / Q000138:chemical synthesis* / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D065546:Drug Liberation; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010316:Particle Size; D011140:Polyurethanes / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D002214:Capsules; D004337:Drug Carriers; D011140:Polyurethanes; D004317:Doxorubicin
keywords: Endo/lysosomes; Intracellular drug release; Microcapsules; Polyurethane; pH-sensitive