title: Rapid Determination of Trace Multiresidues of 18 Sulfonamides in Chicken Eggs Using a Modified QuEChERS Method Coupled with Ultrahigh Performance Liquid Chromatography.
abstract: A modified QuEChERS method was used and an ultrahigh performance liquid chromatography (UHPLC) method was developed for the rapid determination of 18 kinds of sulfonamide residues in chicken eggs. Sample preparation and cleanup conditions were carefully evaluated, and factors such as the adsorbent type and adsorption condition were key parameters in improving the cleanup. The modified QuEChERS method removed matrix interferences, and the sensitivity of the method increased about 5% for recovery and efficiency of the method. Under the optimized UHPLC method with UV detection, all 18 sulfonamide residues were simultaneously separated and rapidly identified within 15 min. The qualitative and quantitative method limits of the 18 sulfonamide residues were 2.06 to 4.12 and 6.86 to 13.7 μg·kg-1, respectively. A close linear relationship (R2= 0.990 to 0.999) was observed within the concentration range of 0.10 to 2.25 μg·ml-1. Recovery was satisfactory (71 to 102%) for all the sulfonamides in three standard spiked levels, with relative standard deviations of <9.7%. After the modified sample pretreatment, the speed of sample pretreatment, purification, and analysis efficiency were all significantly increased. This method is suitable for the rapid detection of multisulfonamide residues in chicken eggs and other animal-derived foods.
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, People's Republic of China.|University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: X U
authors.initials: XU
authors.lastname: Xu
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, People's Republic of China.
authors.forename: Yuan-Can
authors.initials: YC
authors.lastname: Xiao
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, People's Republic of China.
authors.forename: Feng-Zu
authors.initials: FZ
authors.lastname: Hu
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, People's Republic of China.|University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Geng
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis*; D013449:Sulfonamides; D053719:Tandem Mass Spectrometry
chemical_list: D013449:Sulfonamides
keywords: Chicken eggs; QuEChERS; Sulfonamides; Ultrahigh performance liquid chromatography