title: IRF9 as a negative regulator involved in TRIF-mediated NF-κB pathway in a teleost fish, Miichthys miiuy.
abstract: Proinflammatory cytokines and type I IFNs were produced by TLR signaling and these responses are crucial for host defensive responses against pathogens. In order to avoid harmful and inappropriate inflammatory responses, there are multiple mechanisms to negatively regulate TLR signaling. In this paper, we have firstly studied IRF9 functions as a negative regulator involved in TRIF-mediated NF-κB pathway. Moreover, we found inhibitory effect of IRF9 primary depends on DBD domain. Interestingly, we also demonstrated that else mutants of IRF9, except for IRF9-ΔDBD, have different inhibitory effects upon TRIF-mediated NF-κB pathway. This study provides a novel evidence about the negatively regulation of innate immune signaling pathway in teleost fish. In addition, this finding provides new insights into the regulatory mechanism in mammals.
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Cui
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Ruixuan
authors.initials: R
authors.lastname: Huo
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: tianjunxu@163.com.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D007113:Immunity, Innate / Q000276:immunology*; D007150:Immunohistochemistry; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit / Q000276:immunology* / Q000378:metabolism; D016328:NF-kappa B / Q000276:immunology* / Q000378:metabolism; D010473:Perciformes / Q000276:immunology* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit; D016328:NF-kappa B
keywords: IRF9; NF-κB; Negative regulation; TRIF; Teleost