title: An Induced Chromosomal Translocation in Soybean Disrupts aKASIOrtholog and Is Associated with a High-Sucrose and Low-Oil Seed Phenotype.
abstract: Mutagenesis is a useful tool in many crop species to induce heritable genetic variability for trait improvement and gene discovery. In this study, forward screening of a soybean fast neutron (FN) mutant population identified an individual that produced seed with nearly twice the amount of sucrose (8.1% on dry matter basis) and less than half the amount of oil (8.5% on dry matter basis) as compared to wild type. Bulked segregant analysis (BSA), comparative genomic hybridization, and genome resequencing were used to associate the seed composition phenotype with a reciprocal translocation between chromosomes 8 and 13. In a backcross population, the translocation perfectly cosegregated with the seed composition phenotype and exhibited non-Mendelian segregation patterns. We hypothesize that the translocation is responsible for the altered seed composition by disrupting a β-ketoacyl-[acyl carrier protein] synthase 1 (KASI) ortholog.KASIis a core fatty acid synthesis enzyme that is involved in the conversion of sucrose into oil in developing seeds. This finding may lead to new research directions for developing soybean cultivars with modified carbohydrate and oil seed composition.
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.
authors.forename: Austin A
authors.initials: AA
authors.lastname: Dobbels
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.
authors.forename: Jean-Michel
authors.identifier: 0000-0003-3723-2246
authors.initials: JM
authors.lastname: Michno
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.
authors.forename: Benjamin W
authors.identifier: 0000-0002-5510-8583
authors.initials: BW
authors.lastname: Campbell
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.
authors.forename: Kamaldeep S
authors.initials: KS
authors.lastname: Virdi
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.
authors.forename: Adrian O
authors.initials: AO
authors.lastname: Stec
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.|Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108.
authors.forename: Gary J
authors.identifier: 0000-0001-9320-2629
authors.initials: GJ
authors.lastname: Muehlbauer
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108.
authors.forename: Seth L
authors.initials: SL
authors.lastname: Naeve
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108 stup0004@umn.edu.
authors.forename: Robert M
authors.identifier: 0000-0002-8836-2924
authors.initials: RM
authors.lastname: Stupar
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017343:Genes, Plant; D006579:Heterozygote; D006720:Homozygote; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D015203:Reproducibility of Results; D012639:Seeds / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013024:Soybean Oil / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism*; D014178:Translocation, Genetic
chemical_list: D010940:Plant Proteins; D013395:Sucrose; D013024:Soybean Oil
keywords: fast neutron; oil; soybean; sucrose; translocation