title: Temperature Gradient Elution and Superheated Eluents in Chip-HPLC.
abstract: Utilizing temperature as an active parameter for optimization in chip-based liquid chromatography is an important step toward high-speed and high-efficiency separations on the microscale. A device including a low thermal mass micro thermostat and a microfluidic glass chip as central elements were designed and evaluated for maximal heating performance of up to 4.7 °C s-1at up to 200 °C. With this enabling technology, high-speed separations in temperature gradient mode were performed both in common reversed-phase eluents and environmental friendly ethanol-based alternatives.
authors.affiliation: Institute of Analytical Chemistry, Department of Chemistry and Mineralogy, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors.forename: Josef J
authors.initials: JJ
authors.lastname: Heiland
authors.affiliation: Institute of Analytical Chemistry, Department of Chemistry and Mineralogy, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Lotter
authors.affiliation: Fraunhofer ICT-IMM , Carl-Zeiss-Straße 18-20, 55129 Mainz, Germany.
authors.forename: Volkmar
authors.initials: V
authors.lastname: Stein
authors.affiliation: Institute of Analytical Chemistry, Department of Chemistry and Mineralogy, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Mauritz
authors.affiliation: Institute of Analytical Chemistry, Department of Chemistry and Mineralogy, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors.forename: Detlev
authors.identifier: 0000-0001-6295-8706
authors.initials: D
authors.lastname: Belder