title: Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.
abstract: The pathological condition of multiple sclerosis (MS) relies on innate and adaptive immunity. New types of agents that beneficially modify the course of MS, stopping the progression and repairing the damage appear promising. Here, we studied TnP, a small stable synthetic peptide derived from fish venom in the control of inflammation and demyelination in experimental autoimmune encephalomyelitis as prophylactic treatment. TnP decreased the number of the perivascular infiltrates in spinal cord, and the activity of MMP-9 by F4/80+ macrophages were decreased after different regimen treatments. TnP reduces in the central nervous system the infiltration of IFN-γ-producing Th1 and IL-17A-producing Th17 cells. Also, treatment with therapeutic TnP promotes the emergence of functional Treg in the central nervous system entirely dependent on IL-10. Therapeutic TnP treatment accelerates the remyelination process in a cuprizone model of demyelination. These findings support the beneficial effects of TnP and provides a new therapeutic opportunity for the treatment of MS.
authors.affiliation: Immunoregulation Unit, Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Brazil.
authors.forename: Evilin Naname
authors.initials: EN
authors.lastname: Komegae
authors.affiliation: Immunoregulation Unit, Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Brazil.
authors.forename: Tais Aparecida Matozo
authors.initials: TA
authors.lastname: Souza
authors.affiliation: Immunoregulation Unit, Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Brazil.
authors.forename: Lidiane Zito
authors.initials: LZ
authors.lastname: Grund
authors.affiliation: Immunoregulation Unit, Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: Lima
authors.affiliation: Immunoregulation Unit, Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Brazil.
authors.forename: Monica
authors.identifier: http://orcid.org/0000-0003-0244-7482
authors.initials: M
authors.lastname: Lopes-Ferreira
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology; D001938:Brazil; D003471:Cuprizone; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D005260:Female; D005397:Fish Venoms / Q000737:chemistry*; D005786:Gene Expression Regulation; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology; D051379:Mice; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010473:Perciformes / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology
chemical_list: D000943:Antigens, Differentiation; D005397:Fish Venoms; D007155:Immunologic Factors; D020381:Interleukin-17; D010455:Peptides; C036763:monocyte-macrophage differentiation antigen; D016753:Interleukin-10; D003471:Cuprizone; D007371:Interferon-gamma; D020780:Matrix Metalloproteinase 9