title: [Effects of Shock Wave on the Proliferation and Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells].
abstract: OBJECTIVE
  To explore the effects of the shock wave on the capacity of mesenchymal stem cells(MSCs) to proliferate and differentiate into osteoblasts.
  
  
  METHODS
  MSCs were isolated from the bone marrow of healthy donors. The human bone marrow MSCs(BM-MSCs) were divided into 3 groups including blank control group,osteoinduced group and shock wave group. The MSCs in blank control group were cultured with common mediam; the MSCs in osteoinduced group were treated with osteogenic agents and cultured; the MSCs in shock wave group were cultured with common medium and stimulated by shock wave. The morphology of MSCs in each groups were observed by micoscopy; the CCK-8 was used to detect the proliferation ability of MSCs; the alkaline phosphatase staining and von Kossa staining were used to evaluale the differentiation potential of MSCs in each groups.
  
  
  RESULTS
  The results of CCK-8 revealed the shock wave could promote cell proliferation as compared with blank control group. The results of alkaline phosphatase and Von Kossa staining showed that the shock wave displayed a stronger ability to promote the human BMMSC differentiation into osteoblasts cells in comparison with the osteoinduced group. The blank control group was weakly positively stainined.
  
  
  CONCLUSIONS
  The shock wave treatment can promote proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.
authors.affiliation: Department of Orthopedics, Center of Sport Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yi-Meng
authors.initials: YM
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Center of Sport Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Center of Sport Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Wei-Xiong
authors.initials: WX
authors.lastname: Liao
authors.affiliation: Department of Orthopedics, Center of Sport Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiang-Zheng
authors.initials: XZ
authors.lastname: Su
authors.affiliation: Department of Orthopedics, Center of Sport Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Center of Sport Medicine, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ke-Tao
authors.initials: KT
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sicences, Beijing 100850, China.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sicences, Beijing 100850, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Mao
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sicences, Beijing 100850, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Chinese PLA Rocket Force General Hospital, Beijing 100088,China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Qiangdao Century Jiechuang Medi-Tech Corporation, Qingdao 266000, Shandong Province,China.
authors.forename: Gong-Qian
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sicences, Beijing 100850, China. E-mail:zhudingdingabc@163.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, Center of Sport Medicine, Chinese PLA General Hospital, Beijing 100853, China. E-mail:lizhongli@263.net.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Li
mesh_terms: D001854:Bone Marrow Cells; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D010012:Osteogenesis