title: Proteome scale identification, classification and structural analysis of iron-binding proteins in bread wheat.
abstract: Bread wheat is one of the major staple foods of worldwide population and iron plays a significant role in growth and development of the plant. In this report, we are presenting the genome wide identification of iron-binding proteins in bread wheat. The wheat genome derived putative proteome was screened for identification of iron-binding sequence motifs. Out of 602 putative iron-binding proteins, 130 were able to produce reliable structural models by homology techniques and further analyzed for the presence of iron-binding structural motifs. The computationally identified proteins appear to bind to ferrous and ferric ions and showed diverse coordination geometries. Glu, His, Asp and Cys amino acid residues were found to be mostly involved in iron binding. We have classified these proteins on the basis of their localization in the different cellular compartments. The identified proteins were further classified into their protein folds, families and functional classes ranging from structure maintenance of cellular components, regulation of gene expression, post translational modification, membrane proteins, enzymes, signaling and storage proteins. This comprehensive report regarding structural iron binding proteome provides useful insights into the diversity of iron binding proteins of wheat plants and further utilized to study their roles in plant growth, development and physiology.
authors.affiliation: School of Life Sciences, Central University of Himachal Pradesh Kangra, Himachal Pradesh 176 206, India. Electronic address: skverma.cuhl@nic.in.
authors.forename: Shailender Kumar
authors.initials: SK
authors.lastname: Verma
authors.affiliation: School of Life Sciences, Central University of Himachal Pradesh Kangra, Himachal Pradesh 176 206, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Sharma
authors.affiliation: School of Life Sciences, Central University of Himachal Pradesh Kangra, Himachal Pradesh 176 206, India.
authors.forename: Padmani
authors.initials: P
authors.lastname: Sandhu
authors.affiliation: School of Life Sciences, Central University of Himachal Pradesh Kangra, Himachal Pradesh 176 206, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Choudhary
authors.affiliation: School of Life Sciences, Central University of Himachal Pradesh Kangra, Himachal Pradesh 176 206, India.
authors.forename: Shailaja
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, Himachal Pradesh 176 061, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Acharya
authors.affiliation: School of Life Sciences, Central University of Himachal Pradesh Kangra, Himachal Pradesh 176 206, India. Electronic address: yusuf@daad-alumni.de.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Akhter
mesh_terms: D033862:Iron-Binding Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics / Q000378:metabolism
chemical_list: D033862:Iron-Binding Proteins; D010940:Plant Proteins; D020543:Proteome
keywords: Coordination geometries; Ferric and ferrous ions; Iron-binding proteins; Structural bioinformatics; Triticum aestivum