title: SNHG16 contributes to breast cancer cell migration by competitively binding miR-98 with E2F5.
abstract: Long noncoding RNAs (lncRNAs) have been proved to play important roles in cellular processes of cancer, including the development, proliferation, and migration of cancer cells. In the present study, we demonstrated small nucleolar RNA host gene 16 (SNHG16) as an oncogene on cell migration in breast cancer. Expression levels of SNHG16 were found to be frequently higher in breast cancer tissues than in the paired noncancerous tissues. Gain- and loss-of-function studies proved that SNHG16 significantly promoted breast cancer cell migration. We predicted SNHG16 as a competitive endogenous RNA (ceRNA) of E2F transcription factor 5 protein (E2F5) via competition for the shared miR-98 through bioinformatics analysis, and proved this regulation using relative quantitative real-time PCR (qRT-PCR), western blot, RNA immunoprecipitation (RIP) assay and luciferase reporter assay. In addition, we identified a positive correlation between SNHG16 and E2F5 in breast cancer tissues. Furthermore, we demonstrated that forced expression of miR-98 could partially abrogate SNHG16-mediated increase of breast cancer cells migration, suggesting that SNHG16 promoted cell migration in a miR-98 dependent manner. Taken together, our findings indicated that SNHG16 induces breast cancer cell migration by competitively binding miR-98 with E2F5, and SNHG16 can serve as a potential therapeutic target for breast cancer treatment.
authors.affiliation: Department of Breast Surgery, Qilu Hospital of Shandong University, Ji'nan, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Cai
authors.affiliation: Department of Orthopaedic Surgery, Zibo Central Hospital Affiliated to Shandong University, Zibo, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huo
authors.affiliation: Department of Breast Surgery, Qilu Hospital of Shandong University, Ji'nan, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Pathology Tissue Bank, Qilu Hospital of Shandong University, Ji'nan, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Breast Surgery, Qilu Hospital of Shandong University, Ji'nan, China; Pathology Tissue Bank, Qilu Hospital of Shandong University, Ji'nan, China. Electronic address: qifengy_sdu@163.com.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Yang
mesh_terms: D001940:Breast / Q000378:metabolism / Q000473:pathology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D050700:E2F5 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: D050700:E2F5 Transcription Factor; C092558:E2F5 protein, human; C561567:GAS5 long non-coding RNA, human; C546084:MIRN98 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: Breast cancer; E2F5; Migration; SNHG16; ceRNA; miR-98