title: Intrinsic Peroxidase-like Activity of Ficin.
abstract: Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for the intrinsic peroxidase-like activity of ficin and designed experiments to examine its effectiveness in a variety of scenarios. Ficin can transform peroxidase substrates to colored products in the existence of H2O2. Our results also indicate that the active sites of peroxidase-like activity of ficin are different from that of protease, which reveals that one enzyme may catalyze more than one kind of substrate to perform different types of reactions. On the basis of these findings, H2O2releasing from MCF-7 cells was detected successfully. Our findings support a wider application of ficin in biochemistry and open up the possibility of utilizing ficin as enzymatic mimics in biotechnology and environmental monitoring.
authors.affiliation: The key Laboratory on Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: The key Laboratory on Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Dongjun
authors.initials: D
authors.lastname: Shen
authors.affiliation: The key Laboratory on Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Long
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, P. R. China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: The key Laboratory on Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Huzhi
authors.initials: H
authors.lastname: Zheng
mesh_terms: D020134:Catalytic Domain; D005361:Ficain / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D061986:MCF-7 Cells; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide; D009195:Peroxidase; D005361:Ficain