title: Simultaneous cell disruption and lipid extraction in a microalgal biomass using a nonpolar tertiary amine.
abstract: A simultaneous cell disruption and lipid extraction method is developed for microalgal biodiesel production using a triethylamine/methanol solvent system. Individually, the pure solvents, i.e. triethylamine and methanol, do not exhibit significant enhancement in lipid extraction, but a 3:7 (v/v) triethylamine/methanol mixture exhibits the highest lipid extraction, corresponding to 150% of the conventional chloroform/methanol (2:1, v/v) solvent extraction. This extraction is equivalent to 92.5% of the total lipids, even when extracted from a wet microalgal biomass with a water content of 80%. The cell surfaces of the microalgae are significantly disrupted without using additional cell disruption reagents and without requiring energy-intensive equipment. The lipid mass transfer coefficient is 1.6 times greater than that of the chloroform/methanol solvent system. It is clearly demonstrated that triethylamine and methanol cooperate well for the cell disruption and lipid extraction.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-Gu, Guseong-Duong, Daejeon 305-701, Republic of Korea.
authors.forename: Wen-Can
authors.initials: WC
authors.lastname: Huang
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-Gu, Guseong-Duong, Daejeon 305-701, Republic of Korea. Electronic address: kjd@kaist.ac.kr.
authors.forename: Jong-Duk
authors.initials: JD
authors.lastname: Kim
mesh_terms: D000588:Amines / Q000737:chemistry*; D056804:Biofuels; D018533:Biomass; D007700:Kinetics; D008055:Lipids / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry; D058086:Microalgae / Q000166:cytology* / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry
chemical_list: D000588:Amines; D056804:Biofuels; D008055:Lipids; D012997:Solvents; D000432:Methanol
keywords: Biodiesel; Cell disruption; Lipid extraction; Microalgae; Tertiary amine