title: Determining the Effects of Geraniol on Liver Regeneration Via the Nuclear Factor kB Pathway After Partial Hepatectomy.
abstract: Context • Nuclear factor kB (NF-κB) is a dimeric transcription factor that is involved in the regulation of regenerative and apoptosic genes and plays a key role in liver regeneration after a partial hepatectomy (PH). Complementary medicine is used to treat various diseases and can be obtained from a large number of plants that are found in nature. One such plant is geraniol, and no studies have yet occurred assessing its in vivo effects on liver regeneration. Objective • The current study intended to assess the effects of geraniol on liver regeneration after a 70% PH in rats. Design • The research team studied geraniol in a rat model in vivo. Setting • The study took place in the medical and surgical experimental research center at Eskisehir Osmangazi University (Eskisehir, Turkey). Animals • The animals were Wistar albino male rats. Intervention • The rats were divided into 8 groups with 6 rats in each group. Two groups were the sham control groups. The other 6 groups received an injection of a single dose of saline, the negative control; silymarin, the negative control; or geraniol, the intervention. The injections were given intraperitoneally immediately after PH. A laparotomy was performed on the rats all of those groups at either 24 h or 48 h after the PH. Outcome Measures • Using the reverse transcription (RT)- polymerase chain reaction (PCR) method (RT-PCR) and Western blot analysis, the NF-κB, tumor necrosis factor α, and interleukin 6 gene expression and protein levels were measured. Moreover, the levels of the heat shock proteins (HSPs) HSP27 and HSP60 were examined by Western blot. Results • The data showed that geraniol had a significant role (P < .05) in increasing the process of liver regeneration when given intraperitoneally, and it protected the liver as assessed by histology and the HSP levels. In rats receiving 100 mg/kg geraniol intraperitoneally, the agent induced hepatic regeneration 24 h and 48 h after PH (70%).
authors.forename: Emre
authors.initials: E
authors.lastname: Ceyhan
authors.forename: Mediha
authors.initials: M
authors.lastname: Canbek
mesh_terms: D000080462:Acyclic Monoterpenes; D000818:Animals; D006498:Hepatectomy; D015850:Interleukin-6 / Q000032:analysis / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D000080462:Acyclic Monoterpenes; D015850:Interleukin-6; D016328:NF-kappa B; D013729:Terpenes; C007836:geraniol