title: The Area Between Exchange Curves as a Measure of Conformational Differences in Hydrogen-Deuterium Exchange Mass Spectrometry Studies.
abstract: Hydrogen-deuterium exchange mass spectrometry (HDX-MS) provides information about protein conformational mobility under native conditions. The area between exchange curves, Abec, a functional data analysis concept, was adapted to the interpretation of HDX-MS data and provides a useful measure of exchange curve dissimilarity for tests of significance. Importantly, for most globular proteins under native conditions, Abecvalues provide an estimate of the log ratio of exchange-competent fractions in the two states, and thus are related to differences in the free energy of microdomain unfolding. Graphical Abstract ᅟ.
authors.affiliation: Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA. mazurs@mail.nih.gov.
authors.forename: Sharlyn J
authors.initials: SJ
authors.lastname: Mazur
authors.affiliation: Interclypse, Hanover, MD, 21076, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Weber
keywords: Conformational mobility; Functional data analysis; HDX-MS