title: Identification and characterization of a novel diacylglycerol acyltransferase gene from Mortierella alpina.
abstract: OBJECTIVE
  To clone and express a diacylglycerol acyltransferase (DGAT) gene from Mortierella alpina in Saccharomyces cerevisiae and characterize oil production and fatty acid composition of the resulting recombinant RESULTS: A new, full-length cDNA, putatively encoding a DGAT, was cloned from M. alpina. We subsequently cloned the gene, except the transmembrane-encoding region, termed MaDGAT, its molecular mass was 31.3 kDa. MaDGAT shares 75% identity with a DGAT from Mortierella verticillata NRRL 6337. A recombinant vector expressing MaDGAT, pYES2-DGAT, was constructed and transformed into S. cerevisiae H1246, a neutral, lipid-deficient quadruple mutant. TLC analysis showed that the recombinant vector restored triacylglycerol biosynthesis and its content in the recombinant strain was 3.9%.
  
  
  CONCLUSIONS
  MaDGAT is a novel DGAT gene and could increase TAG biosynthesis in M. alpina or other filamentous fungi, thereby promoting the synthesis of polyunsaturated fatty acids.
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.
authors.forename: Xin-An
authors.initials: XA
authors.lastname: Luo
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.
authors.forename: Yuan-Min
authors.initials: YM
authors.lastname: Zhu
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.
authors.forename: Peng-Peng
authors.initials: PP
authors.lastname: Zhou
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Bao
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China. yulongjiang@hust.edu.cn.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China. yulongjiang@hust.edu.cn.
authors.forename: Long-Jiang
authors.initials: LJ
authors.lastname: Yu
mesh_terms: D003001:Cloning, Molecular; D051048:Diacylglycerol O-Acyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D066292:Lipid Droplets / Q000737:chemistry / Q000378:metabolism; D020094:Mortierella / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D014280:Triglycerides; D051048:Diacylglycerol O-Acyltransferase
keywords: Diacylglycerol acyltransferase; Heterologous expression; Mortierella alpina; Saccharomyces cerevisiae; Triacylglycerols