title: Synergistic Antilisterial Effects of Mixtures of Lysozyme and Organic Acids.
abstract: We investigated the synergistic effects of lysozyme combined with organic acids to inhibit the growth of Listeria monocytogenes . The antilisterial effects of the combination of lysozyme and acetic acid, citric acid, lactic acid, malic acid, or succinic acid were evaluated using the checkerboard method and time-kill assay. The MIC was 25,000 mg/liter for lysozyme, 625 mg/liter for acetic acid, and 1,250 mg/liter for the other acids. The MBC was 10,000 mg/liter for all of the tested organic acids. The combination of lysozyme and each organic acid showed synergistic effects via the checkerboard method; however, the time-kill assay showed synergistic effects for only three combinations of 1,250 mg/liter lysozyme with succinic acid (312 and 625 mg/liter) or malic acid (625 mg/liter). The results of this study indicate that the combination of lysozyme and malic acid or succinic acid can be effectively used as a food preservative to control L. monocytogenes .
authors.affiliation: Department of Food Science and Nutrition, Dankook University, Gyeonggi 448-701, Korea.
authors.forename: Myeonggeun
authors.initials: M
authors.lastname: Oh
authors.affiliation: Department of Food Science and Nutrition, Dankook University, Gyeonggi 448-701, Korea.
authors.forename: Joongjae
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, Dankook University, Gyeonggi 448-701, Korea.
authors.forename: Yoonhwa
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: Department of Food Science and Nutrition, Dankook University, Gyeonggi 448-701, Korea.
authors.forename: Misook
authors.initials: M
authors.lastname: Kim
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005520:Food Preservatives / Q000494:pharmacology; D008089:Listeria monocytogenes; D009113:Muramidase / Q000494:pharmacology*
chemical_list: D005520:Food Preservatives; D009113:Muramidase; D019342:Acetic Acid
keywords: Listeria monocytogenes; Lysozyme; Organic acid; Synergistic inhibition