title: Pharmacological Basis for Use ofSelaginella moellendorffiiin Gouty Arthritis: Antihyperuricemic, Anti-Inflammatory, and Xanthine Oxidase Inhibition.
abstract: This study was aimed at evaluating the effects ofSelaginella moellendorffiiHieron. (SM) on gouty arthritis and getting an insight of the possible mechanisms. HPLC method was developed for chemical analysis. The paw oedema, the neutrophil accumulation, inflammatory mediators, lipid peroxidation, and histopathological changes of the joints were analyzed in gouty arthritis rat model, and the kidney injury and serum urate were detected in hyperuricemic mice. Pharmacokinetic result demonstrated that the main apigenin glycosides might be quantitatively transformed into apigenin in the mammalian body. Among these compounds, the apigenin exhibited the strongest effect on xanthine oxidase (XOD). SM aqueous extract has proved to be active in reducing hyperuricemia in dose-dependent manner, and the levels of blood urea nitrogen (BUN) and creatinine (Cr) in high dose group were decreased significantly as compared with hyperuricemic control group (P< 0.01). The high dose of SM extract could significantly prevent the paw swelling, reduce gouty joint inflammatory features, reduce the release of IL-1βand TNF-α, lower malondialdehyde (MDA) and myeloperoxidase (MPO) levels, and increase superoxide dismutase (SOD) level (P< 0.01). For the first time, this study provides a rational basis for the traditional use of SM aqueous extract against gout in folk medicine.
authors.affiliation: Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription and College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription and College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China.
authors.forename: Ke-Li
authors.initials: KL
authors.lastname: Chen
authors.affiliation: Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription and College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China; Department of Pharmacy, Huanggang Hospital of Traditional Chinese Medicine, Huanggang 438000, China.
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription and College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China; Department of Pharmacy, Huanggang Hospital of Traditional Chinese Medicine, Huanggang 438000, China.
authors.forename: Guang-Rui
authors.identifier: 0000-0002-9625-9740
authors.initials: GR
authors.lastname: Deng
authors.affiliation: Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription and College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China; State Key Laboratory of Dao-Di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Juan
authors.identifier: 0000-0002-9838-0990
authors.initials: J
authors.lastname: Li