title: MiR-26a performs converse roles in proliferation and metastasis of different gastric cancer cells via regulating of PTEN expression.
abstract: Gastric cancer is the second leading cause of cancer-related death in the world. The exact molecular pathways in gastric cancer need for further study. We herein indicated miR-26a performed converse roles on oncogenicity in different gastric cancer cells. In gastric cancer cells MKN-28, miR-26a promoted cell proliferation, migration and invasion. However, in gastric cancer cells AGS, miR-26a reduced cell proliferation and metastasis. PTEN was identified as a direct target of miR-26a. In MKN-28 cells, PTEN was suppressed by miR-26a through 3'-UTR, and PTEN mediated miR-26a promoting oncogenicity including cell proliferation and metastasis. On the other hand, in AGS cells, the expression of PTEN was enhanced by miR-26a, and PTEN mediated miR-26a reducing oncogenicity. The mechanism in AGS cells may be the indirect regulation of PTEN by miR-26a overcame the direct targeting regulation. The model like MKN-28 cells was concordant with patients with a high level of miR-26a and a low level of PTEN and patients with a low level of miR-26a and a high level of PTEN which showed lower overall survival (OS); the model like AGS cells was concordant with patients with both high level of miR-26a and PTEN and both low level of miR-26a and PTEN which showed higher OS. These findings will facilitate a better understanding of the functions and mechanisms about miR-26a, miR-26a and PTEN are potential combined biomarkers in patients with gastric cancer.
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Keshuo
authors.initials: K
authors.lastname: Ding
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei, Anhui, People's Republic of China; Department of Pathology, The Fourth Affiliated Hospital of Anhui Medical University, People's Republic of China.
authors.forename: Nana
authors.initials: N
authors.lastname: Wang
authors.affiliation: Laboratory of Pathogenic Microbiology and Immunology, Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei, Anhui, People's Republic of China; Department of Pathology, The Fourth Affiliated Hospital of Anhui Medical University, People's Republic of China.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular Tumor Pathology, School of Life Science, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
authors.forename: Pengxu
authors.initials: P
authors.lastname: Qian
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei, Anhui, People's Republic of China. Electronic address: menggangbl@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Meng
authors.affiliation: Laboratory of Molecular Tumor Pathology, School of Life Science, University of Science and Technology of China, Hefei, Anhui, People's Republic of China. Electronic address: tansheng@mail.ustc.edu.cn.
authors.forename: Sheng
authors.initials: S
authors.lastname: Tan
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000601:surgery
chemical_list: D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C528163:MIRN26 microRNA, human; C494929:PTEN protein, human
keywords: Gastric cancer; Metastasis; MiR-26a; PTEN; Proliferation