title: Stability of plasmid PBR322 in Escherichia coli immobilized on cotton cloth during use as resident inoculum.
abstract: E. coli cells harbouring plasmid pBR322 which confers ampicillin resistance were immobilized on cotton cloth. The resulting film was used as an inoculum in daily repeated batch culture in ampicillin-free medium. During two months, the film was able to produce cultures which, at the late log phase, showed little sensitivity to 10 mg/ml ampicillin. Thus such a bacterial film can effectively be used as an inoculum for the production of recombinant DNA products by means of pBR322 or its derivatives in the absence of ampicillin.
authors.affiliation: Department of Biology and Institute of Biochemistry Carleton University, K1S 5B6, Ottawa, Canada.
authors.forename: Sushama
authors.initials: S
authors.lastname: Joshi
authors.affiliation: Department of Biology and Institute of Biochemistry Carleton University, K1S 5B6, Ottawa, Canada.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamazaki