title: Effects of an alternative oxidase gene on conidia viability under external stresses in Monascus ruber M7.
abstract: Monascus species can produce natural edible pigments and many other bioactive metabolites. In this study, mraox gene (Monascus ruber alternative oxidase) was isolated, sequenced, and replaced in order to investigate the function in resistance of conidia to stressful conditions. The derived protein of the mraox gene consisted of 350 amino acids with a conserved ferritin-like diiron-binding domain at the C-terminus, sharing a high homolog with alternative oxidase proteins in other filamentous fungi. Deletion of mraox gene repressed the conidia germination rate (CGR) when conidia were exposed to H2O2, high temperature (40 and 50 °C) and alkerline buffer (pH8.0), but CGR of mraox-deleted strain was not decreased when the conidia were treated with NaCl, acid buffer (citric acid-dibasic sodium phosphate buffer, pH3) compared to that of the wild-type strain, suggesting that mraox gene is partially responsible for the resistance of conidia to stressful conditions in M. ruber.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, PR China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Shao
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Institute of Quality Standard and Testing Technology for Agro-Products, Hubei Academy of Agricultural Sciences, Hongshan District, Wuhany, Hubei Province, PR China.
authors.forename: Youxiang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, PR China.|Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province, PR China.
authors.forename: Fusheng
authors.initials: F
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal; D050296:Microbial Viability / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D032741:Monascus / Q000201:enzymology / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010860:Pigments, Biological / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D013172:Spores, Fungal / Q000201:enzymology / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D000099188:Alternative Oxidase
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010860:Pigments, Biological; D010940:Plant Proteins; D000099188:Alternative Oxidase
keywords: Monascus ruber; alternative oxidase; conidia germination rate