title: Analysis of Soluble Proteins in NaturalCordyceps sinensisfrom Different Producing Areas by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Two-dimensional Electrophoresis.
abstract: BACKGROUND
  As one of the bioactive components inCordyceps sinensis(CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS.
  
  
  OBJECTIVE
  Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas.
  
  
  METHODS
  Proteins from 26 different producing areas were extracted by Tris-HCl buffer with Triton X-100, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE).
  
  
  RESULTS
  The SDS-PAGE results indicated that the number of protein bands and optical density curves of proteins in 26 CS samples was a bit different. However, the 2-DE results showed that the numbers and abundance of protein spots in protein profiles of 26 samples were obviously different and showed certain association with producing areas.
  
  
  CONCLUSIONS
  Based on the expression values of matched protein spots, 26 batches of CS samples can be divided into two main categories (Tibet and Qinghai) by hierarchical cluster analysis.
  
  
  CONCLUSIONS
  The number of protein bands and optical density curves of proteins in 26Cordyceps sinensissamples were a bit different on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profilesNumbers and abundance of protein spots in protein profiles of 26 samples were obvious different on two-dimensional electrophoresis mapsTwenty-six different producing areas of naturalCordyceps sinensissamples were divided into two main categories (Tibet and Qinghai) by Hierarchical cluster analysis based on the values of matched protein spots.Abbreviations Used: SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis, 2-DE: Two-dimensional electrophoresis,Cordyceps sinensis: CS, TCMs: Traditional Chinese medicines.
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Zuo
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
authors.forename: Feng-Qin
authors.initials: FQ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
authors.forename: Yuan-Jia
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Sunshine lake Pharma Co., Ltd., Guangdong 523850, China.
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Qian
authors.affiliation: Sunshine lake Pharma Co., Ltd., Guangdong 523850, China.
authors.forename: Wen-Jia
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
authors.forename: Zhi-Ning
authors.initials: ZN
authors.lastname: Xia
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
authors.forename: Feng-Qing
authors.initials: FQ
authors.lastname: Yang
keywords:  sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Hierarchical cluster analysis; natural Cordyceps sinensis; soluble protein; two-dimensional electrophoresis