title: [PLGA mesh-collagen hybrid scaffold and tissue-engineered product in substitution urethroplasty: experimental validation].
abstract: Urethral strictures are a pressing issue in modern medicine. Substitution urethroplasty is considered one of the most effective treatment methods. However, despite the surgery showing good results, many problems remain unresolved, one being substitute material deficiency in extensive or recurrent strictures, as well as in cases requiring multistage surgeries, including those used to treat hypospadias. Graft removal also leaves the donor area prone to diseases and increases the length of surgery leading to a higher risk of intra- and postoperative complications. Tissue engineering (namely tissue-engineered products comprised of scaffolds and cells) may be a useful tool in dealing with these issues. The authors assessed the characteristics of a novel hybrid scaffold created from "reconstructed" collagen and a poly(lactic-co-glycolic acid) mesh. The resulting composite product showed good mechanical properties and functional performance. The hybrid scaffold was non-cytotoxic and provided an adequate base for cell adhesion and proliferation. Biodegradation resulted in the scaffold being replaced by urothelium and urethral mucosa. The newly formed tissues possessed adequate structural and functional properties. Only one rabbit out of 12 developed urethral stricture at the site of scaffold implantation. The above-mentioned facts suggest that the novel hybrid scaffold is a promising tissue-engineered product with potential implication in substitution urethroplasty.
authors.affiliation: Sechenov First Moscow State Medical University.
authors.forename: P V
authors.initials: PV
authors.lastname: Glybochko
authors.affiliation: Department of Urology, Faculty of Medicine.
authors.forename: Yu G
authors.initials: YG
authors.lastname: Alyaev
authors.affiliation: Research Institute for Molecular Medicine.
authors.forename: A B
authors.initials: AB
authors.lastname: Shekhter
authors.affiliation: Research Institute for Uronephrology and Reproductive Health.
authors.forename: A Z
authors.initials: AZ
authors.lastname: Vinarov
authors.affiliation: Research Institute for Molecular Medicine.
authors.forename: L P
authors.initials: LP
authors.lastname: Istranov
authors.affiliation: Research Institute for Molecular Medicine.
authors.forename: E V
authors.initials: EV
authors.lastname: Istranova
authors.affiliation: Research Institute for Molecular Medicine.
authors.forename: R K
authors.initials: RK
authors.lastname: Aboyants
authors.affiliation: Research Institute for Molecular Medicine.
authors.forename: A V
authors.initials: AV
authors.lastname: Lyundup
authors.affiliation: Research Institute for Molecular Medicine.
authors.forename: M E
authors.initials: ME
authors.lastname: Krasheninnikov
authors.affiliation: Research Institute for Molecular Medicine.
authors.forename: A E
authors.initials: AE
authors.lastname: Guller
authors.affiliation: Research Institute for Uronephrology and Reproductive Health.
authors.forename: D V
authors.initials: DV
authors.lastname: Butnaru
authors.affiliation: Research Institute for Uronephrology and Reproductive Health.
authors.forename: L V
authors.initials: LV
authors.lastname: Marisov
authors.affiliation: Sechenov First Moscow State Medical University.
authors.forename: D F
authors.initials: DF
authors.lastname: Kantimerov
authors.affiliation: Research Institute for Uronephrology and Reproductive Health.
authors.forename: A V
authors.initials: AV
authors.lastname: Kurkov
authors.affiliation: Sechenov First Moscow State Medical University.
authors.forename: E I
authors.initials: EI
authors.lastname: Safronova
authors.affiliation: Sechenov First Moscow State Medical University.
authors.forename: E A
authors.initials: EA
authors.lastname: Vorobeva
authors.affiliation: Sechenov First Moscow State Medical University.
authors.forename: M M
authors.initials: MM
authors.lastname: Iritsyan
mesh_terms: D000818:Animals; D003094:Collagen; D008297:Male; D011817:Rabbits; D019651:Plastic Surgery Procedures; D023822:Tissue Engineering; D014521:Urethra; D014525:Urethral Stricture / Q000601:surgery; D019459:Urothelium
chemical_list: D003094:Collagen
keywords: regenerative medicine; scaffolds; substitution urethropasty; tissue engineering; tissue-engineered products; urethral strictures