title: Food deprivation and prior anoxic coma have opposite effects on the activity of a visual interneuron in the locust.
abstract: We compared how different metabolic stressors, anoxic coma and food deprivation, affected signaling in neural tissue. We used the locust's Descending Contralateral Movement Detector (DCMD) interneuron because its large axon, high firing frequencies, and rapid conduction velocity make it energetically expensive. We exposed locusts to a 30min anoxic coma or 1day of food deprivation and found contrasting effects on signaling within the axon. After a prior anoxic coma, the DCMD fired fewer high-frequency (>200Hz) action potentials (APs) (Control: 12.4±1.6; Coma: 6.3±0.9) with a reduction in axonal conduction velocity (CV) at all frequencies (∼4-8%) when presented with a standard looming visual stimulus. Prior anoxic coma was also associated with a loss of supernormal conduction by reducing both the number of supernormal APs and the firing frequency with the highest CV. Initially, food deprivation caused a significant increase in the number of low- and high-frequency APs with no differences observed in CV. After controlling for isolation, food deprivation resulted in an increase in high-frequency APs (>200Hz: Control: 17.1±1.7; Food-deprived: 19.9±1.3) and an increase in relative conduction velocity for frequencies >150Hz (∼2%). Action potentials of food-deprived animals had a smaller half-width (Control: 0.45±0.02ms; Food-deprived: 0.40±0.01ms) and decay time (Control: 0.62±0.03ms; Food-deprived: 0.54±0.02ms). Our data indicate that the effects of metabolic stress on neural signaling can be stressor-dependent.
authors.affiliation: Centre for Neuroscience Studies, Queen's University, Kingston, Ontario K7L 3N6, Canada. Electronic address: 13kc18@queensu.ca.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Cross
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Samantha
authors.initials: S
authors.lastname: Britton
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Mangulins
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Tomas G A
authors.initials: TGA
authors.lastname: Money
authors.affiliation: Centre for Neuroscience Studies, Queen's University, Kingston, Ontario K7L 3N6, Canada; Department of Biology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: R Meldrum
authors.initials: RM
authors.lastname: Robertson
mesh_terms: D000200:Action Potentials; D000693:Anaerobiosis; D000818:Animals; D005508:Food Deprivation; D007395:Interneurons / Q000502:physiology*; D048188:Locusta migratoria / Q000502:physiology*; D008297:Male; D014796:Visual Perception
keywords: Axons; DCMD; High-frequency firing; Metabolic stress; Supernormal conduction