title: Loss of Snf5 Induces Formation of an Aberrant SWI/SNF Complex.
abstract: The SWI/SNF chromatin remodeling complex is highly conserved from yeast to human, and aberrant SWI/SNF complexes contribute to human disease. The Snf5/SMARCB1/INI1 subunit of SWI/SNF is a tumor suppressor frequently lost in pediatric rhabdoid cancers. We examined the effects of Snf5 loss on the composition, nucleosome binding, recruitment, and remodeling activities of yeast SWI/SNF. The Snf5 subunit is shown by crosslinking-mass spectrometry (CX-MS) and subunit deletion analysis to interact with the ATPase domain of Snf2 and to form a submodule consisting of Snf5, Swp82, and Taf14. Snf5 promotes binding of the Snf2 ATPase domain to nucleosomal DNA and enhances the catalytic and nucleosome remodeling activities of SWI/SNF. Snf5 is also required for SWI/SNF recruitment by acidic transcription factors. RNA-seq analysis suggests that both the recruitment and remodeling functions of Snf5 are required in vivo for SWI/SNF regulation of gene expression. Thus, loss of SNF5 alters the structure and function of SWI/SNF.
authors.affiliation: Department of Biochemistry and Molecular Biology, Southern Illinois University, Carbondale, IL 62901, USA.
authors.forename: Payel
authors.initials: P
authors.lastname: Sen
authors.affiliation: Institute for Systems Biology, Seattle, WA 98109, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: UT MD Anderson Cancer Center, Smithville, TX 78597, USA; UT MD Anderson Center for Cancer Epigenetics, Smithville, TX 78597, USA.
authors.forename: Arjan
authors.initials: A
authors.lastname: Hada
authors.affiliation: UT MD Anderson Cancer Center, Smithville, TX 78597, USA; UT MD Anderson Center for Cancer Epigenetics, Smithville, TX 78597, USA.
authors.forename: Solomon G
authors.initials: SG
authors.lastname: Hailu
authors.affiliation: Department of Biochemistry and Molecular Biology, Southern Illinois University, Carbondale, IL 62901, USA.
authors.forename: Mekonnen Lemma
authors.initials: ML
authors.lastname: Dechassa
authors.affiliation: UT MD Anderson Cancer Center, Smithville, TX 78597, USA; UT MD Anderson Center for Cancer Epigenetics, Smithville, TX 78597, USA.
authors.forename: Jim
authors.initials: J
authors.lastname: Persinger
authors.affiliation: UT MD Anderson Cancer Center, Smithville, TX 78597, USA.
authors.forename: Sandipan
authors.initials: S
authors.lastname: Brahma
authors.affiliation: UT MD Anderson Cancer Center, Smithville, TX 78597, USA.
authors.forename: Somnath
authors.initials: S
authors.lastname: Paul
authors.affiliation: Institute for Systems Biology, Seattle, WA 98109, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Ranish
authors.affiliation: UT MD Anderson Cancer Center, Smithville, TX 78597, USA; UT MD Anderson Center for Cancer Epigenetics, Smithville, TX 78597, USA. Electronic address: bbartholomew@mdanderson.org.
authors.forename: Blaine
authors.initials: B
authors.lastname: Bartholomew
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D009707:Nucleosomes / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D015003:Yeasts / Q000378:metabolism
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009707:Nucleosomes; D021122:Protein Subunits; D014157:Transcription Factors; D000251:Adenosine Triphosphatases
keywords: BAF47; Chromatin remodeling; INI1; SMARCB1; SWI/SNF; Snf5