title: TheEscherichia coliSerogroup O1 and O2 Lipopolysaccharides Are Encoded by Multiple O-antigen Gene Clusters.
abstract: Escherichia colistrains belonging to serogroups O1 and O2 are frequently associated with human infections, especially extra-intestinal infections such as bloodstream infections or urinary tract infections. These strains can be associated with a large array of flagellar antigens. Because of their frequency and clinical importance, a reliable detection ofE. coliO1 and O2 strains and also the frequently associated K1 capsule is important for diagnosis and source attribution ofE. coliinfections in humans and animals. By sequencing the O-antigen clusters of various O1 and O2 strains we showed that the serogroups O1 and O2 are encoded by different sets of O-antigen encoding genes and identified potentially new O-groups. We developed qPCR-assays to detect the various O1 and O2 variants and the K1-encoding gene. These qPCR assays proved to be 100% sensitive and 100% specific and could be valuable tools for the investigations of zoonotic and food-borne infection of humans with O1 and O2 extra-intestinal (ExPEC) or Shiga toxin-producingE. coli(STEC) strains.
authors.affiliation: IdentyPath Platform, Food Safety Laboratory, Anses, Université Paris-Est Maisons-Alfort, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Delannoy
authors.affiliation: National Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment (BfR)Berlin, Germany; Department of Biology, Chemistry, Pharmacy, Institute for Biology - Microbiology, Freie Universität BerlinBerlin, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.affiliation: CNR Associé Escherichia coli, Service de Microbiologie, Hôpital Robert-DebréParis, France; IAME, UMR 1137, INSERMParis, France; IAME, UMR 1137, University Paris Diderot, Sorbonne Paris CitéParis, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Mariani-Kurkdjian
authors.affiliation: IdentyPath Platform, Food Safety Laboratory, Anses, Université Paris-Est Maisons-Alfort, France.
authors.forename: Aubin
authors.initials: A
authors.lastname: Fleiss
authors.affiliation: CNR Associé Escherichia coli, Service de Microbiologie, Hôpital Robert-DebréParis, France; IAME, UMR 1137, INSERMParis, France; IAME, UMR 1137, University Paris Diderot, Sorbonne Paris CitéParis, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bonacorsi
authors.affiliation: IdentyPath Platform, Food Safety Laboratory, Anses, Université Paris-Est Maisons-Alfort, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Fach
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D000072282:Extraintestinal Pathogenic Escherichia coli / Q000235:genetics; D005517:Foodborne Diseases / Q000382:microbiology; D016695:Glycosyltransferases / Q000235:genetics; D006801:Humans; D008070:Lipopolysaccharides / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics; D005810:Multigene Family; D019081:O Antigens / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D065288:Serogroup; D012703:Serotyping; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics; D015047:Zoonoses / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; D019081:O Antigens; C543926:Wzx protein, E coli; D016695:Glycosyltransferases; C513753:Wzy polymerase, E coli
keywords: E. coli; ExPEC; O-AGC; O-antigen gene cluster; STEC; Shiga toxin-producing E. coli; extraintestinal E. coli; serotyping