title: Role of Periostin in Early Brain Injury After Subarachnoid Hemorrhage in Mice.
abstract: A matricellular protein tenascin-C is implicated in early brain injury after experimental subarachnoid hemorrhage (SAH). This study first evaluated the role of another matricellular protein periostin and the relationships with tenascin-C in post-SAH early brain injury.
  
  
  
  Wild-type (n=226) and tenascin-C knockout (n=9) C57BL/6 male adult mice underwent sham or filament perforation SAH modeling. Vehicle, anti-periostin antibody, or recombinant periostin was randomly administrated by an intracerebroventricular injection at 30 minutes post-modeling. Neuroscores, SAH grading, brain water content, immunostaining, and Western blotting were blindly evaluated at 24 to 48 hours post-SAH.
  
  
  
  Periostin was induced in brain capillary endothelial cells and neurons at 24 hours post-SAH. Anti-periostin antibody improved post-SAH neurobehavior, brain edema, and blood-brain barrier disruption associated with downregulation of tenascin-C, inactivation of p38, extracellular signal-related kinase 1/2 and matrix metalloproteinase-9, and subsequent preservation of zona occludens-1. Recombinant periostin aggravated post-SAH brain edema and tenascin-C induction. Tenascin-C knockout prevented post-SAH neurobehavioral impairments and periostin induction.
  
  
  
  Periostin may cause post-SAH early brain injury through activating downstream signaling pathways and interacting with tenascin-C, providing a novel approach for the treatment of early brain injury.
authors.affiliation: From the Department of Neurosurgery (L.L., F.K., M.F., F.N., H.S.), Department of Pathology and Matrix Biology (K.I.-Y., T.Y.), and Research Center for Matrix Biology (K.I.-Y., T.Y., H.S.), Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: From the Department of Neurosurgery (L.L., F.K., M.F., F.N., H.S.), Department of Pathology and Matrix Biology (K.I.-Y., T.Y.), and Research Center for Matrix Biology (K.I.-Y., T.Y., H.S.), Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Kawakita
authors.affiliation: From the Department of Neurosurgery (L.L., F.K., M.F., F.N., H.S.), Department of Pathology and Matrix Biology (K.I.-Y., T.Y.), and Research Center for Matrix Biology (K.I.-Y., T.Y., H.S.), Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Fujimoto
authors.affiliation: From the Department of Neurosurgery (L.L., F.K., M.F., F.N., H.S.), Department of Pathology and Matrix Biology (K.I.-Y., T.Y.), and Research Center for Matrix Biology (K.I.-Y., T.Y., H.S.), Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Nakano
authors.affiliation: From the Department of Neurosurgery (L.L., F.K., M.F., F.N., H.S.), Department of Pathology and Matrix Biology (K.I.-Y., T.Y.), and Research Center for Matrix Biology (K.I.-Y., T.Y., H.S.), Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Imanaka-Yoshida
authors.affiliation: From the Department of Neurosurgery (L.L., F.K., M.F., F.N., H.S.), Department of Pathology and Matrix Biology (K.I.-Y., T.Y.), and Research Center for Matrix Biology (K.I.-Y., T.Y., H.S.), Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: From the Department of Neurosurgery (L.L., F.K., M.F., F.N., H.S.), Department of Pathology and Matrix Biology (K.I.-Y., T.Y.), and Research Center for Matrix Biology (K.I.-Y., T.Y., H.S.), Mie University Graduate School of Medicine, Tsu, Japan. suzuki02@clin.medic.mie-u.ac.jp.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D000906:Antibodies; D001812:Blood-Brain Barrier / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011994:Recombinant Proteins; D013345:Subarachnoid Hemorrhage / Q000150:complications / Q000378:metabolism*; D019063:Tenascin / Q000378:metabolism*
chemical_list: D000906:Antibodies; D015815:Cell Adhesion Molecules; C082838:Postn protein, mouse; D011994:Recombinant Proteins; D019063:Tenascin
keywords: blood–brain barrier; early brain injury; periostin; subarachnoid hemorrhage; tenascin-C