title: Optical Interference-Free Surface-Enhanced Raman Scattering CO-Nanotags for Logical Multiplex Detection of Vascular Disease-Related Biomarkers.
abstract: Matrix metalloproteinases (MMPs), specifically MMP-2, MMP-7, and MMP-9, have been discovered to be linked to many forms of vascular diseases such as stroke, and their detection is crucial to facilitate clinical diagnosis. In this work, we prepared a class of optical interference-free SERS nanotags (CO-nanotags) that can be used for the purpose of multiplex sensing of different MMPs. Multiplex detection with the absence of cross-talk was achieved by using CO-nanotags with individual tunable intrinsic Raman shifts of CO in the 1800-2200 cm-1region determined by the metal core and ligands of the metal carbonyl complex. Boolean logic was used as well to simultaneously probe for two proteolytic inputs. Such nanotags offer the advantages of convenient detection of target nanotags and high sensitivity as validated in the ischemia rat model.
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China , Chengdu, 610054, P. R. China.
authors.forename: Tianxun
authors.identifier: 0000-0001-9383-3666
authors.initials: T
authors.lastname: Gong
authors.affiliation: Department of Chemistry, National Taiwan University , Taipei, 10617, Taiwan.
authors.forename: Zi-Yao
authors.initials: ZY
authors.lastname: Hong
authors.affiliation: Sustainable Energy Development Center, National Taiwan University of Science and Technology , Taipei, 10607, Taiwan.
authors.forename: Ching-Hsiang
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Chemistry, National Taiwan University , Taipei, 10617, Taiwan.
authors.forename: Cheng-Yen
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes , 35 Keyen Road, Zhunan, Miaoli Country, 35053, Taiwan.
authors.forename: Lun-De
authors.initials: LD
authors.lastname: Liao
authors.affiliation: Department of Chemistry, National Taiwan University , Taipei, 10617, Taiwan.
authors.forename: Kien Voon
authors.identifier: 0000-0002-5910-6497
authors.initials: KV
authors.lastname: Kong
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002248:Carbon Monoxide / Q000737:chemistry*; D008024:Ligands; D008297:Male; D020782:Matrix Metalloproteinases / Q000097:blood* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D055620:Optical Phenomena; D009942:Organometallic Compounds / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D014652:Vascular Diseases / Q000097:blood / Q000000981:diagnostic imaging* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008024:Ligands; D009942:Organometallic Compounds; D002248:Carbon Monoxide; D020782:Matrix Metalloproteinases
keywords: biosensors; metalloproteinases; nanopillars; nanotags; stroke; surface-enhanced Raman spectroscopy; vascular diseases