title: Platelet rich plasma promotes skeletal muscle cell migration in association with up-regulation of FAK, paxillin, and F-Actin formation.
abstract: Platelet rich plasma (PRP) contains various cytokines and growth factors which may be beneficial to the healing process of injured muscle. The aim of this study was to investigate the effect and molecular mechanism of PRP on migration of skeletal muscle cells. Skeletal muscle cells intrinsic to Sprague-Dawley rats were treated with PRP. The cell migration was evaluated by transwell filter migration assay and electric cell-substrate impedance sensing. The spreading of cells was evaluated microscopically. The formation of filamentous actin (F-actin) cytoskeleton was assessed by immunofluorescence staining. The protein expressions of paxillin and focal adhesion kinase (FAK) were assessed by Western blot analysis. Transfection of paxillin small-interfering RNA (siRNAs) to muscle cells was performed to validate the role of paxillin in PRP-mediated promotion of cell migration. Dose-dependently PRP promotes migration of and spreading and muscle cells. Protein expressions of paxillin and FAK were up-regulated dose-dependently. F-actin formation was also enhanced by PRP treatment. Furthermore, the knockdown of paxillin expression impaired the effect of PRP to promote cell migration. It was concluded that PRP promoting migration of muscle cells is associated with up-regulation of proteins expression of paxillin and FAK as well as increasing F-actin formation. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2506-2512, 2017.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taoyuan City, Taiwan.|College of Medicine, Chang Gung University, Taoyuan City, Taiwan.
authors.forename: Wen-Chung
authors.initials: WC
authors.lastname: Tsai
authors.affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taoyuan City, Taiwan.
authors.forename: Tung-Yang
authors.initials: TY
authors.lastname: Yu
authors.affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taoyuan City, Taiwan.|Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan City, Taiwan.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Lin
authors.affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taoyuan City, Taiwan.
authors.forename: Mioa-Sui
authors.initials: MS
authors.lastname: Lin
authors.affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taoyuan City, Taiwan.
authors.forename: Ting-Ta
authors.initials: TT
authors.lastname: Tsai
authors.affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taoyuan City, Taiwan.|Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan City, Taiwan.
authors.forename: Jong-Hwei S
authors.initials: JS
authors.lastname: Pang
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D051419:Paxillin / Q000378:metabolism*; D053657:Platelet-Rich Plasma; D061251:Primary Cell Culture; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: D000199:Actins; D051419:Paxillin; D051417:Focal Adhesion Kinase 1; C496052:Ptk2 protein, rat
keywords: PRP; cell migration; mechanism; platelet-rich plasma; skeletal muscle