title: Oral Ingestion of Collagen Hydrolysate Leads to the Transportation of Highly Concentrated Gly-Pro-Hyp and Its Hydrolyzed Form of Pro-Hyp into the Bloodstream and Skin.
abstract: Collagen hydrolysate is a well-known dietary supplement for the treatment of skin aging; however, its mode of action remains unknown. Previous studies have shown that the oral ingestion of collagen hydrolysate leads to elevated levels of collagen-derived peptides in the blood, but whether these peptides reach the skin remains unclear. Here, we analyzed the plasma concentration of collagen-derived peptides after ingestion of high tripeptide containing collagen hydrolysate in humans. We identified 17 types of collagen-derived peptides transiently, with a particular enrichment in Gly-Pro-Hyp. This was also observed using an in vivo mouse model in the plasma and skin, albeit with a higher enrichment of Pro-Hyp in the skin. Interestingly, this Pro-Hyp enrichment in the skin was derived from Gly-Pro-Hyp hydrolysis, as the administration of pure Gly-Pro-Hyp peptide led to similar results. Therefore, we propose that functional peptides can be transferred to the skin by dietary supplements of collagen.
authors.affiliation: Research Institute, FANCL Corporation , 12-13 Kamishinano, Totsukaku, Yokohama, Kanagawa 244-0806, Japan.|Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University , 3-9 Fuku-ura, Kanazawaku, Yokohama 236-0004, Japan.
authors.forename: Misato
authors.identifier: http://orcid.org/0000-0002-8876-291X
authors.initials: M
authors.lastname: Yazaki
authors.affiliation: Research Institute, FANCL Corporation , 12-13 Kamishinano, Totsukaku, Yokohama, Kanagawa 244-0806, Japan.
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Research Institute, FANCL Corporation , 12-13 Kamishinano, Totsukaku, Yokohama, Kanagawa 244-0806, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University , 3-9 Fuku-ura, Kanazawaku, Yokohama 236-0004, Japan.
authors.forename: Spyros
authors.initials: S
authors.lastname: Goulas
authors.affiliation: Research Institute, FANCL Corporation , 12-13 Kamishinano, Totsukaku, Yokohama, Kanagawa 244-0806, Japan.
authors.forename: Sachiyuki
authors.initials: S
authors.lastname: Teramoto
authors.affiliation: Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University , 3-9 Fuku-ura, Kanazawaku, Yokohama 236-0004, Japan.
authors.forename: Masa-Aki
authors.initials: MA
authors.lastname: Nakaya
authors.affiliation: Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University , 3-9 Fuku-ura, Kanazawaku, Yokohama 236-0004, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohno
authors.affiliation: Research Institute, FANCL Corporation , 12-13 Kamishinano, Totsukaku, Yokohama, Kanagawa 244-0806, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000328:Adult; D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism*; D004151:Dipeptides / Q000097:blood / Q000378:metabolism*; D004435:Eating; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000097:blood / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D004151:Dipeptides; D009842:Oligopeptides; D011492:Protein Hydrolysates; C088188:glycyl-prolyl-hydroxyproline; C533879:prolyl-4-hydroxyproline; D003094:Collagen
keywords: Gly-Pro-Hyp; Pro-Hyp; collagen; dietary supplement; kinetics; peptide