title: Antitumorigenic effect of atmospheric-pressure dielectric barrier discharge on human colorectal cancer cells via regulation of Sp1 transcription factor.
abstract: Human colorectal cancer cell lines (HT29 and HCT116) were exposed to dielectric barrier discharge (DBD) plasma at atmospheric pressure to investigate the anticancer capacity of the plasma. The dose- and time-dependent effects of DBDP on cell viability, regulation of transcription factor Sp1, cell-cycle analysis, and colony formation were investigated by means of MTS assay, DAPI staining, propidium iodide staining, annexin V-FITC staining, Western blot analysis, RT-PCR analysis, fluorescence microscopy, and anchorage-independent cell transformation assay. By increasing the duration of plasma dose times, significant reductions in the levels of both Sp1 protein and Sp1 mRNA were observed in both cell lines. Also, expression of negative regulators related to the cell cycle (such as p53, p21, and p27) was increased and of the positive regulator cyclin D1 was decreased, indicating that the plasma treatment led to apoptosis and cell-cycle arrest. In addition, the sizes and quantities of colony formation were significantly suppressed even though two cancer promoters, such as TPA and epidermal growth factor, accompanied the plasma treatment. Thus, plasma treatment inhibited cell viability and colony formation by suppressing Sp1, which induced apoptosis and cell-cycle arrest in these two human colorectal cancer cell lines.
authors.affiliation: Department of Applied Plasma Engineering, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.
authors.forename: Duksun
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK 21 Plus, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.
authors.forename: Jin Hyoung
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK 21 Plus, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.
authors.forename: Ra Ham
authors.initials: RH
authors.lastname: Lee
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK 21 Plus, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.
authors.forename: Woong
authors.initials: W
authors.lastname: Bang
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK 21 Plus, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.
authors.forename: Kyungho
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of New Biology, DGIST, Daegu 42988, Republic of Korea.
authors.forename: Minseok S
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, 1666 Yeongsan-ro, Muan-gun, Jeonnam, Republic of Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Shim
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK 21 Plus, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Chae
authors.affiliation: Department of Applied Plasma Engineering, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.|Department of Quantum System Engineering, Chonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do, Republic of Korea.
authors.forename: Se Youn
authors.initials: SY
authors.lastname: Moon
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D001274:Atmospheric Pressure; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D058626:Plasma Gases / Q000494:pharmacology*; D016329:Sp1 Transcription Factor / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D058626:Plasma Gases; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human