title: Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate.
abstract: Ammonia-oxidizing bacteria were immobilized by polyvinyl alcohol (PVA) and sodium alginate. The immobilization conditions and ammonia oxidation ability of the immobilized bacteria were investigated. The following immobilization conditions were observed to be optimal: PVA, 12%; sodium alginate, 1.1%; calcium chloride, 1.0%; inoculum concentration, 1.3 immobilized balls/mL of immobilized medium; pH, 10; and temperature, 30°C. The immobilized ammonia-oxidizing bacteria exhibited strong ammonia oxidation ability even after being recycled four times. The ammonia nitrogen removal rate of the immobilized ammonia-oxidizing bacteria reached 90.30% under the optimal immobilization conditions. When compared with ammonia-oxidizing bacteria immobilized by sodium alginate alone, the bacteria immobilized by PVA and sodium alginate were superior with respect to pH resistance, the number of reuses, material cost, heat resistance, and ammonia oxidation ability.
authors.affiliation: Xuzhou University of Technology, Xuzhou, PR China; China University of Mining and Technology, Xuzhou, PR China. Electronic address: 258210902@qq.com.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Dong
authors.affiliation: China University of Mining and Technology, Xuzhou, PR China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Xuzhou University of Technology, Xuzhou, PR China; China University of Mining and Technology, Xuzhou, PR China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Tu
mesh_terms: D000464:Alginates / Q000737:chemistry; D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D010084:Oxidation-Reduction; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013696:Temperature
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D000641:Ammonia; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol
keywords: Ammonia oxidation; Ammonia-oxidizing bacteria; Immobilization; Polyvinyl alcohol; Sodium alginate