title: TiNxOycoatings facilitate the initial adhesion of osteoblasts to create a suitable environment for their proliferation and the recruitment of endothelial cells.
abstract: Titanium-nitride-oxide coatings (TiNxOy) improve osseointegration of endosseous implants. The exact mechanisms by which these effects are mediated are poorly understood except for an increase of osteoblast proliferation while a high degree of differentiation is maintained. One hypothesis holds that TiNxOyfacilitates the initial spreading and adhesion of the osteoblasts. The aim of this work was to investigate the molecular mechanisms of osteoblast adhesion on TiNxOyas compared to microrough titanium SLA. A global view of the osseointegrative process, that is, taking into account other cell groups, especially endothelial cells, is also presented. To this aim, gene expression and focal adhesion analysis, cocultures and wound assays were performed early after seeding, from 6 h to 3 days. We demonstrated that TiNxOycoatings enhance osteoblast adhesion and spreading when compared to the standard microrough titanium. The integrin β1, either in association with α1 or with α2 plays a central role in these mechanisms. TiNxOycoatings optimize the process of osseointegration by acting at several levels, especially by upregulating osteoblast adhesion and proliferation, but also by supporting neovascularization and the development of a suitable inflammatory environment.
authors.affiliation: Division of fixed prosthodontics and biomaterials, University clinics of dental medicine, University of Geneva, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Moussa
authors.forename: O
authors.initials: O
authors.lastname: Banakh
authors.forename: B
authors.initials: B
authors.lastname: Wehrle-Haller
authors.forename: P
authors.initials: P
authors.lastname: Fontana
authors.forename: S
authors.initials: S
authors.lastname: Scherrer
authors.forename: M
authors.initials: M
authors.lastname: Cattani
authors.forename: A
authors.initials: A
authors.lastname: Wiskott
authors.forename: S
authors.initials: S
authors.lastname: Durual
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D018920:Coculture Techniques; D016207:Cytokines / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D018836:Inflammation Mediators / Q000502:physiology; D016023:Integrins / Q000502:physiology; D008422:Materials Testing; D018919:Neovascularization, Physiologic; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D016207:Cytokines; D018836:Inflammation Mediators; D016023:Integrins; C434243:titanium-nitride-oxide; D014025:Titanium