title: Exposure to sodium molybdate results in mild oxidative stress in Drosophila melanogaster.
abstract: OBJECTIVE
  The study was conducted to assess the redox status of Drosophila flies upon oral intake of insulin-mimetic salt, sodium molybdate (Na2MoO4).
  
  
  METHODS
  Oxidative stress parameters and activities of antioxidant and associated enzymes were analyzed in two-day-old D. melanogaster insects after exposure of larvae and newly eclosed adults to three molybdate levels (0.025, 0.5, or 10 mM) in the food.
  
  
  RESULTS
  Molybdate increased content of low molecular mass thiols and activities of catalase, superoxide dismutase, glutathione-S-transferase, and glucose-6-phosphate dehydrogenase in males. The activities of these enzymes were not affected in females. Males exposed to molybdate demonstrated lower carbonyl protein levels than the control cohort, whereas females at the same conditions had higher carbonyl protein content and catalase activity than ones in the control cohort. The exposure to 10 mM sodium molybdate decreased the content of protein thiols in adult flies of both sexes. Sodium molybdate did not affect the activities of NADP-dependent malate dehydrogenase and thioredoxin reductase in males or NADP-dependent isocitrate dehydrogenase in either sex at any concentration.
  
  
  CONCLUSIONS
  Enhanced antioxidant capacity in upon Drosophila flies low molybdate levels in the food suggests that molybdate can be potentially useful for the treatment of certain pathologies associated with oxidative stress.
authors.affiliation: a Department of Biochemistry and Biotechnology , Vasyl Stefanyk Precarpathian National University , Ivano-Frankivsk , Ukraine.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Perkhulyn
authors.affiliation: a Department of Biochemistry and Biotechnology , Vasyl Stefanyk Precarpathian National University , Ivano-Frankivsk , Ukraine.
authors.forename: Bohdana M
authors.initials: BM
authors.lastname: Rovenko
authors.affiliation: a Department of Biochemistry and Biotechnology , Vasyl Stefanyk Precarpathian National University , Ivano-Frankivsk , Ukraine.
authors.forename: Oleh V
authors.initials: OV
authors.lastname: Lushchak
authors.affiliation: b Institute of Biochemistry , Carleton University , Ottawa , Canada.
authors.forename: Janet M
authors.initials: JM
authors.lastname: Storey
authors.affiliation: b Institute of Biochemistry , Carleton University , Ottawa , Canada.
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Storey
authors.affiliation: a Department of Biochemistry and Biotechnology , Vasyl Stefanyk Precarpathian National University , Ivano-Frankivsk , Ukraine.
authors.forename: Volodymyr I
authors.initials: VI
authors.lastname: Lushchak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000254:growth & development; D005260:Female; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D008982:Molybdenum / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D008982:Molybdenum; C024687:sodium molybdate(VI)
keywords: Fruit fly; antioxidant enzymes; antioxidants; oxidative stress