title: PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance.
abstract: Leucine-rich repeats receptor-like kinases (LRR-RLKs) play important roles in plant growth and development as well as stress responses. Here, 56 LRR-RLK genes were identified in the Antarctic moss Pohlia nutans transcriptome, which were further classified into 11 subgroups based on their extracellular domain. Of them, PnLRR-RLK27 belongs to the LRR II subgroup and its expression was significantly induced by abiotic stresses. Subcellular localization analysis showed that PnLRR-RLK27 was a plasma membrane protein. The overexpression of PnLRR-RLK27 in Physcomitrella significantly enhanced the salinity and ABA tolerance in their gametophyte growth. Similarly, PnLRR-RLK27 heterologous expression in Arabidopsis increased the salinity and ABA tolerance in their seed germination and early root growth as well as the tolerance to oxidative stress. PnLRR-RLK27 overproduction in these transgenic plants increased the expression of salt stress/ABA-related genes. Furthermore, PnLRR-RLK27 increased the activities of reactive oxygen species (ROS) scavengers and reduced the levels of malondialdehyde (MDA) and ROS. Taken together, these results suggested that PnLRR-RLK27 as a signaling regulator confer abiotic stress response associated with the regulation of the stress- and ABA-mediated signaling network.
authors.affiliation: School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China.
authors.forename: Shenghao
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan, China.
authors.forename: Tailin
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan, China.|Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Jinan, China.
authors.forename: Pengying
authors.identifier: http://orcid.org/0000-0002-9515-084X
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan, China.
authors.forename: Kaoshan
authors.initials: K
authors.lastname: Chen
mesh_terms: D000864:Antarctic Regions; D017360:Arabidopsis / Q000378:metabolism; D019068:Bryopsida / Q000201:enzymology*; D002462:Cell Membrane / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000031:analogs & derivatives; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D018525:Germination; D006861:Hydrogen Peroxide / Q000737:chemistry; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010446:Peptide Fragments; D010940:Plant Proteins / Q000502:physiology*; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000201:enzymology; D011392:Proline / Q000737:chemistry; D011494:Protein Kinases / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012492:Salts / Q000737:chemistry; D012639:Seeds / Q000502:physiology; D015398:Signal Transduction; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D059467:Transcriptome
chemical_list: C083591:H-R corticotropin-releasing factor (12-41), Phe(12)-Nle(21,38), C(alpha-MeLeu(37))-; D010446:Peptide Fragments; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D012492:Salts; D008315:Malondialdehyde; D003346:Corticotropin-Releasing Hormone; D011392:Proline; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D011494:Protein Kinases