title: In vitro kinetic investigations on the bioactivity and cytocompatibility of bioactive glasses prepared via melting and sol-gel techniques for bone regeneration applications.
abstract: This work investigates and compares the influence of the synthesis process on the in vitro bioactivity of two quaternary bioactive glasses prepared via melting and sol-gel (SG) techniques. The two glasses are named MG and SG, respectively. Powder samples were soaked in simulated body fluid for different time intervals to study the kinetics of Ca and P uptake onto their surface as well as Si release. The uptake kinetics followed the pseudo-second order model, and the kinetic parameters in addition to the initial rates were estimated. MG manifested higher Ca uptake capacity than SG which could be attributed to the presence of a residual organic layer capping the surface of SG, as was confirmed by Fourier transform infrared and nuclear magnetic resonance analyses. However, higher rate of Ca uptake was exhibited by SG probably due to its higher reactivity that resulted from its smaller nano-size and higher negative charge as was evident from transmission electron microscopy and dynamic light scattering measurements, respectively. Furthermore, MG showed slightly higher P uptake capacity and lower amount of Si release. Initial rates of Ca and P uptakes onto SG as well as Si release from SG exceeded those of MG. Human bone osteosarcoma cells (Saos-2) were co-cultured with both MG and SG glasses and the latter showed higher alkaline phosphatase activity and higher cell growth induction. The results showed the promising potential of using both bioactive glasses in bone regeneration. However, the choice of the appropriate bioactive glass depends on the targeted applications.
authors.affiliation: Chemical Engineering Department, National Research Centre (NRC), El Bohouth St., Dokki, 12622 Cairo, Egypt. Chemistry Department, American University in Cairo, New Cairo, 11835, Egypt. Nanomedicine & Tissue Engineering Lab., Medical Research Center of Excellence (MRCE), NRC, Cairo, Egypt.
authors.forename: Mayyada M H
authors.initials: MM
authors.lastname: El-Sayed
authors.forename: Amany A
authors.initials: AA
authors.lastname: Mostafa
authors.forename: Alaa M
authors.initials: AM
authors.lastname: Gaafar
authors.forename: Walid
authors.initials: W
authors.lastname: El Hotaby
authors.forename: Esmat Ma
authors.initials: EM
authors.lastname: Hamzawy
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: El-Okaily
authors.forename: Amira M
authors.initials: AM
authors.lastname: Gamal-Eldeen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry* / Q000302:isolation & purification; D001861:Bone Regeneration / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005782:Gels; D005898:Glass / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D005782:Gels; C570103:bioactive glass 63S; D000469:Alkaline Phosphatase