title: [Effect of Icaritin on Proliferation and Apoptosis of THP-1 Cell and Its Mechanism].
abstract: OBJECTIVE
  To investigate the effect of icaritin on the proliferation and apoptosis of THP-1 cells and its mechanism.
  
  
  METHODS
  After treated with various concentrations of icaritin, cell proliferation was detected by MTS method, and apoptosis was measured with flow cytometry and Hoechst 33258 staining. Expression of BCL-2, BAX and Caspase-3 protein in THP-1 cell was detected by Western blot.
  
  
  RESULTS
  After treatment with various concentrations (4-32 µmol/L) of icaritin for 24, 48, 72 h, the inhibition rate of cell growth significantly increased (P<0.05) in time-dose dependent manner(r=0.946); and the apoptotic rate of cells significantly increased (P<0.05) in time-dose dependent manner(r= 0.924). The expression of BCL-2 protein at 48 h decreased significantly in icaritin-treated group, compared with that in control group (P<0.05), while the expression of BAX and Caspase3 protein at 48 h increased significantly in icaritin-treated group, compared with that in control group (P<0.05).
  
  
  CONCLUSIONS
  Icaritin can inhibit proliferation and induce apoptosis of THP-1 in vitro, Icaritin may induce apoptosis in THP-1 cells through the mitochondrial pathway.
authors.affiliation: Department of Hematology,Hunan Provincial Children's Hospital,Changsha 410007,Hunan Province,China.
authors.forename: Wen-Yong
authors.initials: WY
authors.lastname: Kuang
authors.affiliation: Department of Hematology,Hunan Provincial Children's Hospital,Changsha 410007,Hunan Province,China.
authors.forename: Min-Cui
authors.initials: MC
authors.lastname: Zheng
authors.affiliation: Department of Hematology,Second Xiangya Hospital,Central South University, Changsha 410011,Hunan Province,China.
authors.forename: Guang-Sen
authors.initials: GS
authors.lastname: Zhang
authors.affiliation: Department of Hematology,Second Xiangya Hospital,Central South University, Changsha 410011,Hunan Province,China. E-mail:26572652@qq.com.
authors.forename: Rui-Juan
authors.initials: RJ
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D000074084:THP-1 Cells
chemical_list: D005419:Flavonoids; D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3; C499403:icaritin