title: 1H,13C and15N backbone chemical shift assignments of camelid single-domain antibodies against active state µ-opioid receptor.
abstract: Nanobodies are single chain antibodies that have become a highly valuable and versatile tool for biomolecular and therapeutic research. One application field is the stabilization of active states of flexible proteins, among which G-protein coupled receptors represent a very important class of membrane proteins. Here we present the backbone and side-chain assignment of the1H,13C and15N resonances of Nb33 and Nb39, two nanobodies that recognize and stabilize the µ-opioid receptor to opioids in its active agonist-bound conformation. In addition, we present a comparison of their secondary structures as derived from NMR chemical shifts.
authors.affiliation: Institut de Genomique Fonctionnelle (IGF), CNRS, INSERM, Univ. Montpellier, F-34094, Montpellier, France. remy.sounier@igf.cnrs.fr.
authors.forename: Remy
authors.initials: R
authors.lastname: Sounier
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR 5048-INSERM 1054, University of Montpellier, 29 rue de Navacelles, 34090, Montpellier Cedex, France.
authors.forename: Yinshan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institut de Genomique Fonctionnelle (IGF), CNRS, INSERM, Univ. Montpellier, F-34094, Montpellier, France.
authors.forename: Joanna
authors.initials: J
authors.lastname: Hagelberger
authors.affiliation: Institut de Genomique Fonctionnelle (IGF), CNRS, INSERM, Univ. Montpellier, F-34094, Montpellier, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Granier
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR 5048-INSERM 1054, University of Montpellier, 29 rue de Navacelles, 34090, Montpellier Cedex, France. helene.demene@cbs.cnrs.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Déméné
mesh_terms: D000818:Animals; D000071194:Camelidae; D019906:Nuclear Magnetic Resonance, Biomolecular; D017450:Receptors, Opioid, mu / Q000276:immunology*; D061905:Single-Domain Antibodies / Q000737:chemistry* / Q000276:immunology*
chemical_list: D017450:Receptors, Opioid, mu; D061905:Single-Domain Antibodies
keywords: Camelid antibody; G protein coupled receptor; Nanobody