title: The immediate early protein WSV187 can influence viral replication via regulation of JAK/STAT pathway in Drosophila.
abstract: The world production of shrimp is seriously affected by the white spot syndrome virus (WSSV). Viral immediate-early (IE) genes encode regulatory proteins critical for the viral lifecycle. In spite of their importance, only five out of the 21 identified WSSV IE genes are functionally characterized. Here, we report the use of Drosophila melanogaster as a model to explore the role of WSSV IE gene wsv187. In vivo expression of WSV187 in transgenic flies show WSV187 localized in the cytoplasm. Overexpression of wsv187 results wing defects consistent with phenotypes observed in JAK/STAT exacerbated flies. After artificial infection of the DCV virus, the flies expressing wsv187 showed a lower viral load, a higher survival rate and an up-regulated STAT92E expression. These data demonstrate wsv187 plays a role in the controlling of virus replication by activating host JAK/STAT pathway.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China; State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
authors.forename: Changkun
authors.initials: C
authors.lastname: Pan
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China. Electronic address: wangw.sz@tio.org.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Yuan
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
authors.forename: Baoru
authors.initials: B
authors.lastname: Chen
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China.
authors.forename: Dengfeng
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China. Electronic address: chenjm@xmu.edu.cn.
authors.forename: Jianming
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D004266:DNA Virus Infections / Q000276:immunology*; D004331:Drosophila melanogaster / Q000276:immunology*; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D007113:Immunity, Innate; D053612:Janus Kinases / Q000378:metabolism; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D019562:Viral Load; D014779:Virus Replication / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D017874:Immediate-Early Proteins; D050791:STAT Transcription Factors; D053612:Janus Kinases
keywords: Immediate-early gene; JAK/STAT signaling; Viral replication; WSSV; wsv187