title: Quantitative Proteomics of the Root of Transgenic Wheat ExpressingTaBWPR-1.2Genes in Response to Waterlogging.
abstract: Once candidate genes are available, the application of genetic transformation plays a major part to study their function in plants for adaptation to respective environmental stresses, including waterlogging (WL). The introduction of stress-inducible genes into wheat remains difficult because of low transformation and plant regeneration efficiencies and expression variability and instability. Earlier, we found two cDNAs encoding WL stress-responsive wheat pathogenesis-related proteins 1.2 (TaBWPR-1.2),TaBWPR-1.2#2andTaBWPR-1.2#13. Using microprojectile bombardment, both cDNAs were introduced into "Bobwhite". Despite low transformation efficiency, four independent T₂ homozygous lines for each gene were isolated, where transgenes were ubiquitously and variously expressed. The highest transgene expression was obtained inUbi:TaBWPR-1.2#2L#11a andUbi:TaBWPR-1.2#13L#4a. Using quantitative proteomics, the root proteins of L#11a were analyzed to explore possible physiological pathways regulated by TaBWPR-1.2 under normal and waterlogged conditions. In L#11a, the abundance of proteasome subunit alpha type-3 decreased under normal conditions, whereas that of ferredoxin precursor and elongation factor-2 increased under waterlogged conditions in comparison with normal plants. Proteomic results suggest that L#11a is one of the engineered wheat plants where TaBWPR-1.2#2 is most probably involved in proteolysis, protein synthesis and alteration in the energy pathway in root tissues via the above proteins in order to gain metabolic adjustment to WL.
authors.affiliation: NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. haque@yokohama-cu.ac.jp.
authors.forename: Emdadul
authors.initials: E
authors.lastname: Haque
authors.affiliation: NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. fabe@affrc.go.jp.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Abe
authors.affiliation: NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. mmmori@obihiro.ac.jp.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Mori
authors.affiliation: NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. ynanjo@affrc.go.jp.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Nanjo
authors.affiliation: NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. skomatsu@affrc.go.jp.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
authors.affiliation: NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. oyanagi@affrc.go.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Oyanagi
authors.affiliation: NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. kentaro@affrc.go.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kawaguchi
keywords: pathogenesis-related protein-1.2; proteomics; seminal roots; transgenic wheat; waterlogging