title: Variations of Soil Microbial Community Structures Beneath Broadleaved Forest Trees in Temperate and Subtropical Climate Zones.
abstract: Global warming has shifted climate zones poleward or upward. However, understanding the responses and mechanism of microbial community structure and functions relevant to natural climate zone succession is challenged by the high complexity of microbial communities. Here, we examined soil microbial community in three broadleaved forests located in the Wulu Mountain (WLM, temperate climate), Funiu Mountain (FNM, at the border of temperate and subtropical climate zones), or Shennongjia Mountain (SNJ, subtropical climate). Although plant species richness decreased with latitudes, the microbial taxonomic α-diversity increased with latitudes, concomitant with increases in soil total and available nitrogen and phosphorus contents. Phylogenetic NRI (Net Relatedness Index) values increased from -0.718 in temperate zone (WLM) to 1.042 in subtropical zone (SNJ), showing a shift from over dispersion to clustering likely caused by environmental filtering such as low pH and nutrients. Similarly, taxonomy-based association networks of subtropical forest samples were larger and tighter, suggesting clustering. In contrast, functional α-diversity was similar among three forests, but functional gene networks of the FNM forest significantly (P< 0.050) differed from the others. A significant correlation (R= 0.616,P< 0.001) between taxonomic and functional β-diversity was observed only in the FNM forest, suggesting low functional redundancy at the border of climate zones. Using a strategy of space-for-time substitution, we predict that poleward climate range shift will lead to decreased microbial taxonomic α-diversities in broadleaved forest.
authors.affiliation: Institute of Forestry Ecology, Environment and Protection, and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, the Chinese Academy of ForestryBeijing, China; State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityBeijing, China.
authors.forename: Sihang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Forestry Ecology, Environment and Protection, and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, the Chinese Academy of Forestry Beijing, China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Forestry Ecology, Environment and Protection, and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, the Chinese Academy of ForestryBeijing, China; School of Minerals Processing and Bioengineering, Central South UniversityChangsha, China; Department of Oncology, the Affiliated Hospital of Qingdao UniversityQingdao, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cong
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University Beijing, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University Beijing, China.
authors.forename: Mengxin
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institute of Forestry Ecology, Environment and Protection, and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, the Chinese Academy of Forestry Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lu
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University Beijing, China.
authors.forename: Changyi
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma Norman, OK, USA.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma Norman, OK, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Yuan
authors.affiliation: Institute of Forestry Ecology, Environment and Protection, and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, the Chinese Academy of Forestry Beijing, China.
authors.forename: Diqiang
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityBeijing, China; Institute for Environmental Genomics and Department of Botany and Microbiology, University of OklahomaNorman, OK, USA; Earth Sciences Division, Lawrence Berkeley National LaboratoryBerkeley, CA, USA.
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Environmental Sciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.
authors.forename: Baohua
authors.initials: B
authors.lastname: Gu
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University Beijing, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yang
keywords: GeoChip; broadleaved forests; high-throughput seuqencing; microbial community; soil biogeochemical process