title: Exercise partially reverses the inhibitory effect of caffeine on liver gluconeogenesis in type 1 diabetic rats with hypoglycemia.
abstract: The purpose was to determine the possible effects of exercise and/or caffeine on hypoglycemia and liver gluconeogenesis in diabetic rats. These were divided into four subgroups: (a) intraperitoneal insulin only, (b) exercise bout before insulin, (c) caffeine after insulin, and (d) exercise bout before and caffeine after insulin. The marked glycemic drop 45 min after insulin (0 min = 229.00, 45 min = 75.75) was considerably reduced (p < 0.05) by caffeine or exercise (45 min: exercise = 127.00, caffeine = 104.78). However, this systemic effect was lost (p > 0.05) when they were combined (45 min: exercise + caffeine = 65.44) (Mean, in mg·dL-1). Caffeine alone strongly inhibited liver glucose production from 2 mM lactate 45 min after insulin (without caffeine = 3.05, with caffeine = 0.27; p < 0.05), while exercise + caffeine partially re-established the liver gluconeogenic capacity (exercise + caffeine = 1.61; p < 0.05 relative to the other groups) (Mean, in μmol·g-1). The improved hypoglycemia with caffeine or exercise cannot be explained by their actions on liver gluconeogenesis. As their beneficial effect disappeared when they were combined, such association in diabetic patients should be avoided during the period of hyperinsulinemia due to the risk of severe hypoglycemia.
authors.affiliation: 1 Department of Biochemistry, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: E H
authors.initials: EH
authors.lastname: Gilglioni
authors.affiliation: 2 Department of Physiological Sciences, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Ghuidotti
authors.affiliation: 1 Department of Biochemistry, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: V R
authors.initials: VR
authors.lastname: Vilela
authors.affiliation: 2 Department of Physiological Sciences, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Bataglini
authors.affiliation: 3 Department of Physical Education, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: J P
authors.initials: JP
authors.lastname: Furlan
authors.affiliation: 2 Department of Physiological Sciences, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: J A
authors.initials: JA
authors.lastname: Berti
authors.affiliation: 2 Department of Physiological Sciences, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: Mmd
authors.initials: M
authors.lastname: Pedrosa
authors.affiliation: 2 Department of Physiological Sciences, State University of Maringá , Maringá, Paraná, Brazil.
authors.forename: Vaf
authors.initials: V
authors.lastname: Godoi
mesh_terms: D000818:Animals; D002110:Caffeine / Q000009:adverse effects*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation / Q000187:drug effects; D005943:Gluconeogenesis / Q000187:drug effects*; D007003:Hypoglycemia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002110:Caffeine
keywords: caffeine; exercise; insulin; liver; rats; type 1 diabetes