title: [Analysis of the Detection Results of the Syphilis Specific Antibody in Blood Donors by Chemiluminescence Method and Enzyme Linked Immunosorbent Assay].
abstract: OBJECTIVE
  To investigate the application value of chemiluminescence method (CMIA) detection of Treponema pallidum (TP) specific antibodies in the blood test.
  
  
  METHODS
  Over the same period the de novo enzyme linked immunosorbent assay (ELISA) and Abbott chemical luminescence method were used to detect the specific antibody of syphilis in a total of 66298 samples; TP-ELISA negative and TP-CMIA positive unpaid blood donation blood samples for syphilis specific antibody were detected and confirmed by Western blot.
  
  
  RESULTS
  Blood samples from 66298 blood donors were detected by TP-ELISA, the positive samples was 250 and the positive rate was 0.38%. The positive samples of TP-CMIA was 297, the positive rate was 0.45%, the difference was statistically significant (P<0.05). The blood samples of 47 unpaid blood donors were confirmed by TP-Western blot method, as a result, 32 samples were positive, 15 were negative, and result detected by TP-ELISA method was negative.
  
  
  CONCLUSIONS
  TP-CMIA sensitivity is higher than that of TP-ELISA, and possesses higher sensitivity and specificity, and quick detection, simple operation, easy automation, suggesting greater application value in blood detection of Treponema pallidum.
authors.affiliation: Dongying Blood Center, Dongying 257091, Shandong Province, China. E-mail:men.shou.shan@163.com.
authors.forename: Shou-Shan
authors.initials: SS
authors.lastname: Men
authors.affiliation: Dongying Blood Center, Dongying 257091, Shandong Province, China.
authors.forename: Fa-Kui
authors.initials: FK
authors.lastname: Shang
authors.affiliation: Dongying Blood Center, Dongying 257091, Shandong Province, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Han
authors.affiliation: Dongying Hospital of Shangdong Province-owned Hospital, Dongying 257091, Shandong Province, China.
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Song
authors.affiliation: Dongying Hospital of Shangdong Province-owned Hospital, Dongying 257091, Shandong Province, China.
authors.forename: Jing-Yin
authors.initials: JY
authors.lastname: Han
mesh_terms: D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D049449:Luminescence; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000175:diagnosis*; D013588:Syphilis Serodiagnosis; D014210:Treponema pallidum