title: IL-1β directly suppress ghrelin mRNA expression in ghrelin-producing cells.
abstract: In animal models, ghrelin production is suppressed by LPS administration. To elucidate the detailed molecular mechanisms involved in the phenomenon, we investigated the effects of LPS and LPS-inducible cytokines, including TNF-α, IL-1β, and IL-6, on the expression of ghrelin in the ghrelin-producing cell line MGN3-1. These cells expressed IL-1R, and IL-1β significantly suppressed ghrelin mRNA levels. The suppressive effects of IL-1β were attenuated by knockdown of IKKβ, suggesting the involvement of the NF-κB pathway. These results suggested that IL-1β is a major regulator of ghrelin expression during inflammatory processes.
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Bando
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan. Electronic address: hiwaku@wakayama-med.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwakura
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ueda
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ariyasu
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Inaba
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Furukawa
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Furuta
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishi
authors.affiliation: The First Department of Medicine, Wakayama Medical University, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Akamizu
mesh_terms: D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D054439:Ghrelin / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
chemical_list: D054439:Ghrelin; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
keywords: Ghrelin; IL-1β