title: Functional Characterization of a New Tea (Camellia sinensis) Flavonoid Glycosyltransferase.
abstract: Tea (Camellia sinensis) is an important commercial crop, in which the high content of flavonoids provides health benefits. A flavonoid glycosyltransferase (CsUGT73A20), belonging to cluster IIIa, was isolated from tea plant. The recombinant CsUGT73A20 in Escherichia coli exhibited a broad substrate tolerance toward multiple flavonoids. Among them, kaempferol was the optimal substrate compared to quercetin, myricetin, naringenin, apigenin, and kaempferide. However, no product was detected when UDP-galactose was used as the sugar donor. The reaction assay indicated that rCsUGT73A20 performed multisite glycosidation toward flavonol compounds, mainly forming 3-O-glucoside and 7-O-glucoside in vitro. The biochemical characterization analysis of CsUGT73A20 showed more K7G product accumulated at pH 8.0, but K3G was the main product at pH 9.0. Kinetic analysis demonstrated that high pH repressed the glycosylation reaction at the 7-OH site in vitro. Besides, the content of five flavonol-glucosides was increased in CsUGT73A20-overexpressing tobaccos (Nicotiana tabacum).
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.|School of Life Science, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Xianqian
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Peiqiang
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Mingzhuo
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Life Science, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Yeru
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Lilan
authors.initials: L
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Juhua
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: School of Life Science, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, China.
authors.forename: Tao
authors.identifier: http://orcid.org/0000-0003-0814-2567
authors.initials: T
authors.lastname: Xia
mesh_terms: D028241:Camellia sinensis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D044950:Flavanones / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism*; D016695:Glycosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D044949:Kaempferols / Q000378:metabolism; D007700:Kinetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D044950:Flavanones; D005419:Flavonoids; D044949:Kaempferols; D010940:Plant Proteins; C449720:kaempferide; C006552:kaempferol; D016695:Glycosyltransferases; C005273:naringenin
keywords: Camellia sinensis; flavonoids glycosyltransferase; multisite glycosidation