title: [Microbiota of urine and vagina of healthy postmenopausal women (a pilot study)].
abstract: Studying microbiota of different urogenital tract habitats in healthy postmenopausal women is of practical importance in deciding on the appropriateness of correction of dysbiotic disorders. The aim of this study was to examine the vaginal and urine microbiota of healthy postmenopausal women. The study included 20 healthy postmenopausal women (mean age 59,0+/-2,1 years). Duration of menopause in all subjects was more than 8 years. Bacteriological testing of urine and vaginal specimen was carried out on the extended media (15) for cultivating facultative anaerobic bacteria (FAB) and nonclostridial anaerobic bacteria (NAB) and included PCR of midstream morning urine. Among FAB in the urine and vagina dominated coagulase-negative staphylococci and NAB. Bacterial patterns of studied habitats turned out to be similar in many respects. In the urine Megasphaera spp., Veillonella spp., Prevotella spp., Mobiluncus spp., Fusobacterium spp. were found, whereas in the vagina these microorganisms were not present. Cluster analysis revealed no significant differences in the concentration of the same microorganisms isolated from the urine and vagina. When comparing the frequency of microorganism detection in urine by bacteriological method and by PCR, bacterial patterns were identical in 56% of cases.
authors.affiliation: Department of Microbiology and Virology 1, Rostov State Medical University.
authors.forename: Yu L
authors.initials: YL
authors.lastname: Naboka
authors.affiliation: Department of Obstetrics and Gynecology 1, Rostov State Medical University.
authors.forename: A N
authors.initials: AN
authors.lastname: Rymashevsky
authors.affiliation: Department of Urology and Human Reproductive Health with Course of Pediatric Urology-Andrology, School for Advanced Training and Professional Retraining of Specialists of Rostov State Medical University.
authors.forename: M I
authors.initials: MI
authors.lastname: Kogan
authors.affiliation: Department of Microbiology and Virology 1, Rostov State Medical University.
authors.forename: I A
authors.initials: IA
authors.lastname: Gudima
authors.affiliation: Department of Obstetrics and Gynecology 1, Rostov State Medical University.
authors.forename: O A
authors.initials: OA
authors.lastname: Borovleva
authors.affiliation: Department of Microbiology and Virology 1, Rostov State Medical University.
authors.forename: K T
authors.initials: KT
authors.lastname: Jalagonia
authors.affiliation: Laboratory of Bioinformatics, Medical Statistics and Computer Modeling of CRL, Rostov-on-Don.
authors.forename: S A
authors.initials: SA
authors.lastname: Zarutskiy
mesh_terms: D001419:Bacteria / Q000145:classification / Q000254:growth & development*; D005260:Female; D006801:Humans; D064307:Microbiota; D008875:Middle Aged; D010865:Pilot Projects; D017698:Postmenopause / Q000652:urine*; D014621:Vagina / Q000382:microbiology*
keywords: microbiota; urine; vagina