title: Mitochondrial Apoptosis Induced byChamaemelum NobileExtract in Breast Cancer Cells.
abstract: Chamaemelum nobile(Asteraceae) commonly known as 'Roman chamomile' is a medicinal plant used for numerous diseases in traditional medicine, although its anticancer activity is unknown. The present study was carried out to investigate the anticancer as well as apoptotic activity of ethyl acetate fraction ofC. nobileon different cancerous cell lines. The cells were treated with varying concentrations (0.001- 0.25 mg/mL) of this fraction for 24, 48 and 72 h. Apoptosis induced in MCF-7 cells following treatment with ethyl acetate fraction was measured using Annexin V/PI, flowcytometry and western blotting analysis. The results showed thatC. nobileethyl acetate fraction revealed relatively high antiproliferative activity on MCF-7 cells; however, it caused minimal growth inhibitory response in normal cells. The involvement of apoptosis as a major cause of the fraction-induced cell death was confirmed by annexin-V/PI assay. In addition, ethyl acetate fraction triggered the mitochondrial apoptotic pathway by decreasing the Bcl-2 as well as increasing of Bax protein expressions and subsequently increasing Bax/Bcl-2 ratio. Furthermore, decreased proliferation of MCF-7 cells in the presence of the fraction was associated with G2/M phase cell cycle arrest. These findings confirm that ethyl acetate fraction ofC.nobilemay contain a diversity of phytochemicals which suppress the proliferation of MCF-7 cells by inducing apoptosis.
authors.affiliation: Physiology and Pharmacology Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Hirsa
authors.initials: H
authors.lastname: Mostafapour Kandelous
authors.affiliation: Physiology and Pharmacology Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Misha
authors.initials: M
authors.lastname: Salimi
authors.affiliation: Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Khori
authors.affiliation: Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Noushin
authors.initials: N
authors.lastname: Rastkari
authors.affiliation: National Cell Bank of Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Amanzadeh
authors.affiliation: Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.; Physiology and Pharmacology Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Salimi
keywords: Apoptosis; Cancer; Chamaemelum nobile; Mitochondria; Proliferation