title: Plasma biomarkers for the identification of women at risk for early-onset preeclampsia.
abstract: To identify potential biomarkers in the 1st trimester of pregnancy for the identification of women destined to develop early onset preeclampsia (EOPE).
  
  
  
  Blood samples were obtained from pregnant women at 11-13 weeks of gestation. Women were followed up until delivery. Five samples from EOPE complicated pregnancies and 5 from unaffected ones were analysed using 2-DE and MALDI-TOF-TOF MS/MS. The altered expression of selected proteins was verified by ELISA in an extended sample cohort.
  
  
  
  Twelve proteins were differentially expressed in the plasma of women who subsequently developed EOPE as compared to controls. Alpha-1-antitrypsin (A1AT), CD5 antigen-like molecule (CD5L) Keratin, type I cytoskeletal 9 (K1C9), Myeloid cell nuclear differentiation antigen (MNDA), Transferrin (TRFE) and Vitamin D-binding protein (VTDB) were up-regulated with fold changes 3.14, 2.18, 1.53, 1.53, 4.26 3.38 respectively, whereas Alpha-2-HS-glycoprotein (FETUA), Beta-2-glycoprotein 1 (APOH), Complement factor B (CFAB), Haptoglobin (HPT), Vitronectin (VTNC) and Zinc-alpha-2-glycoprotein (ZA2G) were down-regulated with fold changes -0.38, -0.76, -0.24, -0.47, -0.23, and -0.50 respectively. The down-regulation of APOH, VTNC and HPT was verified using ELISA.
  
  
  
  The differentially expressed proteins represent potential biomarkers for the early screening for EOPE. Follow-up experiments however are necessary for evaluation.
authors.affiliation: a 3rd Department of Obstetrics Gynecology , Athens University school of Medicine , Athens , Greece.|b Department of Medical Genetics , Athens University school of Medicine , Athens , Greece.
authors.forename: Aggeliki
authors.initials: A
authors.lastname: Kolialexi
authors.affiliation: c Proteomics Research Unit , Biomedical Research Foundation of the Academy of Athens , Athens , Greece.
authors.forename: George Th
authors.initials: GT
authors.lastname: Tsangaris
authors.affiliation: d Department of Obstetrics & Gynecology , University of Crete , Heraklion , Greece.
authors.forename: Stavros
authors.initials: S
authors.lastname: Sifakis
authors.affiliation: e 2nd Department of Paediatrics , Athens University school of Medicine , Athens , Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Gourgiotis
authors.affiliation: c Proteomics Research Unit , Biomedical Research Foundation of the Academy of Athens , Athens , Greece.
authors.forename: Aggeliki
authors.initials: A
authors.lastname: Katsafadou
authors.affiliation: a 3rd Department of Obstetrics Gynecology , Athens University school of Medicine , Athens , Greece.|b Department of Medical Genetics , Athens University school of Medicine , Athens , Greece.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lykoudi
authors.affiliation: e 2nd Department of Paediatrics , Athens University school of Medicine , Athens , Greece.
authors.forename: Antonios
authors.initials: A
authors.lastname: Marmarinos
authors.affiliation: a 3rd Department of Obstetrics Gynecology , Athens University school of Medicine , Athens , Greece.
authors.forename: Danai
authors.initials: D
authors.lastname: Mavreli
authors.affiliation: a 3rd Department of Obstetrics Gynecology , Athens University school of Medicine , Athens , Greece.
authors.forename: Vassilis
authors.initials: V
authors.lastname: Pergialiotis
authors.affiliation: a 3rd Department of Obstetrics Gynecology , Athens University school of Medicine , Athens , Greece.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Fexi
authors.affiliation: b Department of Medical Genetics , Athens University school of Medicine , Athens , Greece.
authors.forename: Ariadni
authors.initials: A
authors.lastname: Mavrou
authors.affiliation: a 3rd Department of Obstetrics Gynecology , Athens University school of Medicine , Athens , Greece.
authors.forename: George K
authors.initials: GK
authors.lastname: Papaioanou
authors.affiliation: a 3rd Department of Obstetrics Gynecology , Athens University school of Medicine , Athens , Greece.
authors.forename: Nikolas
authors.initials: N
authors.lastname: Papantoniou
mesh_terms: D000328:Adult; D017668:Age of Onset; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics / Q000473:pathology; D011247:Pregnancy; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: 2-DE; Preeclampsia; biomarkers; early onset preeclampsia; mass spectrometry; proteomics