title: NMR resonance assignments of the FKBP domain of human aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) in complex with a farnesyl ligand.
abstract: Aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) is a specialized chaperone of phosphodiesterase 6, a key effector enzyme in the phototransduction cascade. The FKBP domain of AIPL1 is known to bind the farnesyl moiety of PDE6. Mutations in AIPL1, including many missense mutations in the FKBP domain, have been associated with Leber congenital amaurosis, a severe blinding disease. Here, we report the backbone and sidechain assignments of the N-terminal FKBPΔloop(with a loop deletion) of AIPL1 in complex with a farnesyl ligand. We also compare the predicted secondary structures of FKBPΔloopwith those of a highly homologous AIP FKBP. These results show that the FKBP domains of AIP and AIPL1 have similar folds, but display subtle differences in structure and dynamics. Therefore, these assignments provide a framework for further elucidation of the mechanism of farnesyl binding and the function of AIPL1 FKBP.
authors.affiliation: Departments of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA. liping-yu@uiowa.edu.|NMR Core Facility, Carver College of Medicine, University of Iowa, 285 Newton Road, Iowa City, IA, 52242, USA. liping-yu@uiowa.edu.
authors.forename: Liping
authors.initials: L
authors.lastname: Yu
authors.affiliation: Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, 51 Newton Road, Iowa City, IA, 52242, USA.
authors.forename: Ravi P
authors.initials: RP
authors.lastname: Yadav
authors.affiliation: Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, 51 Newton Road, Iowa City, IA, 52242, USA. nikolai-artemyev@uiowa.edu.|Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA. nikolai-artemyev@uiowa.edu.
authors.forename: Nikolai O
authors.initials: NO
authors.lastname: Artemyev
mesh_terms: D054707:Cyclic Nucleotide Phosphodiesterases, Type 6 / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000378:metabolism*; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D012717:Sesquiterpenes / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D012717:Sesquiterpenes; C103265:aryl hydrocarbon receptor-interacting protein; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6
keywords: AIP; AIPL1; Chaperone; FKBP; Phosphodiesterase 6