title: Outbreak of dengue virus type-3 in Malakand, Pakistan 2015; A laboratory perspective.
abstract: An outbreak of dengue fever was reported in Malakand district, Khyber Pakhtunkhwa (KP) province of Pakistan during 2015. Detection of viral RNA by real-time PCR confirmed dengue virus serotype-3 (DENV-3) to be the causative agent causing the outbreak. Phylogenetic analysis based on partial E-NS1 gene sequences showed that the DENV-3 viruses belonged to genotype III with maximum homology with the dengue-3 strains previously reported from Pakistan and India. Our current report provides updated information on molecular epidemiology and phylogenetic analysis of dengue virus serotypes responsible for 2015 outbreak in KP.
authors.affiliation: Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan; Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Suleman
authors.affiliation: Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Rani
authors.initials: R
authors.lastname: Faryal
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Muhammad Masroor
authors.initials: MM
authors.lastname: Alam
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Adnan
authors.initials: A
authors.lastname: Khurshid
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Salmaan
authors.initials: S
authors.lastname: Sharif
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Shahzad
authors.initials: S
authors.lastname: Shaukat
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Mehar
authors.initials: M
authors.lastname: Angez
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Massab
authors.initials: M
authors.lastname: Umair
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Mian Muhammad
authors.initials: MM
authors.lastname: Sufian
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Yasir
authors.initials: Y
authors.lastname: Arshad
authors.affiliation: District Health office Malakand, Government of KPK, Pakistan.
authors.forename: Tanveer
authors.initials: T
authors.lastname: Inam
authors.affiliation: Department of Virology, National Institute of Health,Park Road, Chak Shahzad, Islamabad, Pakistan. Electronic address: sohailz@live.com.
authors.forename: Syed Sohail Zahoor
authors.initials: SSZ
authors.lastname: Zaidi
mesh_terms: D003715:Dengue / Q000453:epidemiology*; D003716:Dengue Virus / Q000145:classification*; D004196:Disease Outbreaks; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D007753:Laboratories; D017720:Molecular Epidemiology; D010154:Pakistan / Q000453:epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D065288:Serogroup
chemical_list: D012367:RNA, Viral
keywords: Epidemics; Epidemiology; Infection; Public health