title: Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken.
abstract: Antimicrobials are sometimes given to food animals at low doses in order to promote faster growth. However, the mechanisms by which those drugs improve performance are not fully understood. This study aimed to investigate the impact of zinc bacitracin (55g/ton), enramycin (10g/ton); halquinol® (30g/ton); virginiamycin (16,5g/ton) and avilamycin (10g/ton) on the cecal microbiota of broiler chicken, compared to a control group. Six hundred and twenty four chicks (Cobb 500) arriving to an experimental unit were randomly assigned into each treatment with four repetitions per treatment. The cecal content of 16 animals per treatment (n = 96) was used for DNA extraction and sequencing of the V4 region of the 16S rRNA gene using Illumina technology. The use of antimicrobials induced significant changes in membership but not in structure of the cecal microbiota compared to the control group, suggesting a greater impact on the less abundant species of bacteria present in that environment. Halquinol was the only drug that did not affect microbial membership. Firmicutes comprised the major bacterial phylum present in the cecum of all groups. There was no statistical difference in relative abundances of the main phyla between treated animals and the control group (all P>0.05). Treatment with enramycin was associated with decreased richness and with lower relative abundance of unclassified Firmicutes, Clostridium XI, unclassified Peptostreptococcaceae (all P<0.001) and greater abundance of Clostridium XIVb (P = 0.004) and Anaerosporobacter spp. (P = 0.015), and treatment with bacitracin with greater relative abundance of Bilophila spp. (P = 0.004). Several bacterial genera were identified as representative of usage of each drug. This study used high throughput sequencing to characterize the impact of several antimicrobials in broiler chicken under controlled conditions and add new insights to the current knowledge on how AGPs affect the cecal microbiota of chicken.
authors.affiliation: Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: Marcio C
authors.identifier: http://orcid.org/0000-0001-5067-7501
authors.initials: MC
authors.lastname: Costa
authors.affiliation: Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Bessegatto
authors.affiliation: Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: Amauri A
authors.initials: AA
authors.lastname: Alfieri
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: J Scott
authors.initials: JS
authors.lastname: Weese
authors.affiliation: Department of Animal Science, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: João A B
authors.initials: JA
authors.lastname: Filho
authors.affiliation: Department of Animal Science, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Oba
mesh_terms: D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001414:Bacitracin / Q000494:pharmacology; D001419:Bacteria / Q000145:classification / Q000187:drug effects; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000254:growth & development / Q000382:microbiology*; D002739:Chloroquinolinols / Q000494:pharmacology; D005503:Food Additives / Q000494:pharmacology*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D014644:Genetic Variation; D009844:Oligosaccharides / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D025341:Principal Component Analysis; D011897:Random Allocation; D021521:Ribotyping; D014769:Virginiamycin / Q000494:pharmacology; D010456:Peptides, Cyclic
chemical_list: D000900:Anti-Bacterial Agents; D001414:Bacitracin; D002739:Chloroquinolinols; D005503:Food Additives; D009844:Oligosaccharides; D010455:Peptides; D014769:Virginiamycin; C044447:enramycin; D010456:Peptides, Cyclic; C006653:avilamycin; C011838:halquinol