title: Early Effect of Amyloidβ-Peptide on Hippocampal and Serum Metabolism in Rats Studied by an Integrated Method of NMR-Based Metabolomics and ANOVA-Simultaneous Component Analysis.
abstract: Amyloidβ(Aβ) deposition has been implicated in the pathogenesis of Alzheimer's disease. However, the early effect of Aβdeposition on metabolism remains unclear. In the present study, thus, we explored the metabolic changes in the hippocampus and serum during first 2 weeks of Aβ25-35injection in rats by using an integrated method of NMR-based metabolomics and ANOVA-simultaneous component analysis (ASCA). Our results show that Aβ25-35injection, time, and their interaction had statistically significant effects on the hippocampus and serum metabolome. Furthermore, we identified key metabolites that mainly contributed to these effects. After Aβ25-35injection from 1 to 2 weeks, the levels of lactate, N-acetylaspartate, creatine, and taurine were decreased in rat hippocampus, while an increase in lactate and decreases in LDL/VLDL and glucose were observed in rat serum. Therefore, we suggest that the reduction in energy and lipid metabolism as well as an increase in anaerobic glycolysis may occur at the early stage of Aβ25-35deposition.
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Du
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Hong
authors.identifier: 0000-0002-3401-721X
authors.initials: H
authors.lastname: Zheng
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Xia
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Hu
authors.affiliation: Radiology Department, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Bai
authors.affiliation: Radiology Department, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Zhihan
authors.identifier: 0000-0001-6826-7439
authors.initials: Z
authors.lastname: Yan
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Hongchang
authors.identifier: 0000-0003-3462-1879
authors.initials: H
authors.lastname: Gao
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008568:Memory / Q000187:drug effects; D055432:Metabolomics; D010446:Peptide Fragments / Q000633:toxicity*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D017207:Rats, Sprague-Dawley
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35)