title: Role of Plasticity Region Genes and cagE gene of cagPAI of Helicobacter pylori in Development of Gastrointestinal (GI) Diseases
abstract: Background: Helicobacter pylori is a Gram-negative, micro aerophilic bacterium in the human stomach that is
  associated with the development of gastrointestinal ailments such as peptic ulcer (PU) and gastric cancer (GC). In the
  present study, plasticity region genes (jhp0940, jhp0945 and jhp0947) and and cagE gene of cagPAI were assessed
  independently and in combination for their ability to predict clinical consequences. Materials and Methods: A total of
  211 strains which were isolated from patients with different gastrointestinal diseases (114 with non-atrophic gastritis,
  59 with PU, and 38 with GC) were genotyped by PCR and sequencing. Data were collected and analyzed using SPSS
  software version 19. Logistic regression models were applied to determine relationships between the plasticity region
  genes and cagE of H.pylori and clinical status. Results: The cagE gene (71.1%) had the highest frequency and jhp0945
  (13.7%) was the least abundant among the genes examined. The jhp0940 gene was significantly associated with GC (P
  = 0.0007), but not PU. On multiple logistic regression analysis, adjusted for both age and sex, the jhp0940 genotype
  was significantly associated with GC (odds ratio, OR = 2.8, 95%CI = 1.1–7.0; P = 0.027). The jhp0940+/ jhp0945+/
  jhp0947+genotype was also linked to an increased risk of GC (OR = 50.4, 95%CI = 5.1–500.0; P = 0.0008) while no
  genotype correlation was found with PU in Iran (P > 0.05). Conclusions: Given the high frequency of cagE, this gene
  could be a suitable marker for the presence of cagPAI in Iranian strains. The jhp0940 genotype could also be a strong
  predictor of GC in Iran.
authors.affiliation: Department of Biology, Faculty of Sciences, University of MohagheghArdabili, Ardabil, Iran. Email: golizades@yahoo.com
authors.forename: Shokufe
authors.initials: Sh
authors.lastname: GholizadeTobnagh
authors.forename: Seyedeh Zahra
authors.initials: SZ
authors.lastname: Bakhti
authors.forename: Saeid
authors.initials: S
authors.lastname: Latifi Navid
authors.forename: Saber
authors.initials: S
authors.lastname: Zahri
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Sadat Bakhti
keywords: Helicobacter pylori; plasticity region genes; cagE; gastrointestinal diseases; Iran