title: Chemical profiling of Fufang-Xialian-Capsule by UHPLC-Q-TOF-MS and its antioxidant activity evaluated by in vitro method.
abstract: Fufang Xialian (FXL) capsule, a traditional Chinese medicine (TCM) formula, has been used for a long time to treat Chronic Atrophic Gastritis, but the pharmacodynamic material basis and mechanism of action are unclear. In this research, ferric-reducing antioxidant power (FRAP) method and DPPH scavenging method were utilized to evaluate the antioxidant activity of FXL in vitro. An ultra high-performance liquid chromatography coupled with Linear Ion Trap Mass Spectrometer (UHPLC-IT-MS) method and an ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method were utilized to acquire the constituents information of FXL. Mass spectra were acquired in both positive and negative ion modes. As the results, FXL showed a moderate DPPH scavenging activity and ferric-reducing antioxidant power. A total of 73 components were identified or tentatively characterized in FXL, which included alkaloids, flavones, flavone glycosides, ginsenoside, lignins, triterpene saponins, flavanones, chalcones and coumarin. The results in this research could provide a basis for further study in bioactivity and metabolites.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Shizhe
authors.initials: S
authors.lastname: Li
authors.affiliation: National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: mslab20@ciac.ac.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.affiliation: National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Pi
authors.affiliation: National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Fengrui
authors.initials: F
authors.lastname: Song
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: jinyr@jlu.edu.cn.
authors.forename: Yongri
authors.initials: Y
authors.lastname: Jin
authors.affiliation: National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000470:Alkaloids / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D036145:Ginsenosides / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D008516:Medicine, Chinese Traditional / Q000379:methods; D012503:Saponins / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods; D014315:Triterpenes / Q000737:chemistry
chemical_list: D000470:Alkaloids; D000975:Antioxidants; D002214:Capsules; D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D036145:Ginsenosides; D006027:Glycosides; D012503:Saponins; D014315:Triterpenes; C043562:flavone
keywords: Antioxidant activity; Chemical profiling; Fufang-Xialian-Capsule; UHPLC–MS