title: Correlation between receptor-interacting protein 140 expression and directed differentiation of human embryonic stem cells into neural stem cells.
abstract: Overexpression of receptor-interacting protein 140 (RIP140) promotes neuronal differentiation of N2a cellsviaextracellular regulated kinase 1/2 (ERK1/2) signaling. However, involvement of RIP140 in human neural differentiation remains unclear. We found both RIP140 and ERK1/2 expression increased during neural differentiation of H1 human embryonic stem cells. Moreover, RIP140 negatively correlated with stem cell markers Oct4 and Sox2 during early stages of neural differentiation, and positively correlated with the neural stem cell marker Nestin during later stages. Thus, ERK1/2 signaling may provide the molecular mechanism by which RIP140 takes part in neural differentiation to eventually affect the number of neurons produced.
authors.affiliation: Department of Pediatrics, Peking University People's Hospital, Beijing, China.
authors.forename: Zhu-Ran
authors.initials: ZR
authors.lastname: Zhao
authors.affiliation: Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yu
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Peking University International Hospital, Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Pediatrics, Peking University People's Hospital, Beijing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, Peking University People's Hospital, Beijing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Mu
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Pediatrics, Peking University People's Hospital, Beijing, China.
authors.forename: Jing-Zhu
authors.identifier: 0000-0003-1720-8927
authors.initials: JZ
authors.lastname: Guo
keywords: Nestin; Oct4; Sox2; directed differentiation; extracellular regulated kinase 1/2 signaling pathway; human embryonic stem cells; nerve regeneration; neural regeneration; neural stem cells; receptor-interacting protein 140