title: Ratiometric Imaging of Tissue by Two-Photon Microscopy: Observation of a High Level of Formaldehyde around Mouse Intestinal Crypts.
abstract: Ratiometric imaging by two-photon microscopy can offer a viable tool for the relative quantification of biological analytes inside tissue with minimal influence from environmental factors that affect fluorescence signal. We demonstrate the ratiometric imaging of formaldehyde at the suborgan level using a two-photon fluorescent probe, which involves pixel-to-pixel ratiometric data transformation. This study reveals for the first time a high level of formaldehyde around the crypts of mouse small intestine, implicating its possible protective role along with the released antimicrobials from the Paneth cells.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-Ro, Nam-Gu, Pohang 37673, Republic of Korea.
authors.forename: Subhankar
authors.initials: S
authors.lastname: Singha
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-Ro, Nam-Gu, Pohang 37673, Republic of Korea.
authors.forename: Yong Woong
authors.initials: YW
authors.lastname: Jun
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-Ro, Nam-Gu, Pohang 37673, Republic of Korea.
authors.forename: Juryang
authors.initials: J
authors.lastname: Bae
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-Ro, Nam-Gu, Pohang 37673, Republic of Korea.
authors.forename: Kyo Han
authors.identifier: 0000-0001-7192-7215
authors.initials: KH
authors.lastname: Ahn
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D005557:Formaldehyde / Q000032:analysis*; D007421:Intestine, Small / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D061848:Optical Imaging; D017785:Photons
chemical_list: D005456:Fluorescent Dyes; D005557:Formaldehyde