title: Anti-inflammatory activity of the essential oil obtained from Ocimum basilicum complexed with β-cyclodextrin (β-CD) in mice.
abstract: Cyclodextrins (CDs) are cyclic oligosaccharides can enhance the bioavailability of drugs. Ocimum basilicum is an aromatic plant found in Brazil used in culinary. The essential oil of this plant presents anti-edematogenic and anti-inflammatory activities in acute and chronic inflammation. The aim of this study was to investigate the anti-inflammatory effects of the essential oil obtained from O. basilicum complexed with β - cyclodextrin (OBEO/β-CD) in mice. The complexation with β-cyclodextrin (β-CD) was performed by different methods and analyzed by differential scanning calorimetry (DSC), thermogravimetry (TG) and scanning electron microscopy (SEM). The anti-inflammatory activity was evaluated using mice models of paw edema induced by carrageenan, dextran, histamine and arachidonic acid (AA); vascular permeability and peritonitis induced by carrageenan and granuloma induced by cotton block introduction. The DSC, TG and SEM analysis indicated that the OBEO was successfully complexed with β-CD. The oral administration of OEOB/β-CD prevented paw edema formation by decreasing vascular permeability in vivo, inhibited leukocyte recruitment to the peritoneal cavity, and inhibited granuloma formation in mice. Our results indicate that conjugation with β-CD improves the anti-inflammatory effects of OBEO in mice models of acute and chronic inflammation, indicating that this complex can be used in anti-inflammatory drug development.
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: lindaianefisio@hotmail.com.
authors.forename: Lindaiane Bezerra
authors.initials: LB
authors.lastname: Rodrigues
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: anitaoliveira24@yahoo.com.br.
authors.forename: Anita Oliveira Brito Pereira Bezerra
authors.initials: AOBPB
authors.lastname: Martins
authors.affiliation: Leão Sampaio University Center, CEP 63040-005, Juazeiro do Norte, Ceará, Brazil. Electronic address: jaimeribeirofilho@gmail.com.
authors.forename: Jaime
authors.initials: J
authors.lastname: Ribeiro-Filho
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: rafa._san@hotmail.com.
authors.forename: Francisco Rafael Alves Santana
authors.initials: FRAS
authors.lastname: Cesário
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: fyama_fc@hotmail.com.
authors.forename: Fyama Ferreira
authors.initials: FF
authors.lastname: E Castro
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: thaysrodrigues_albuquerque@hotmail.com.
authors.forename: Thaís Rodrigues
authors.initials: TR
authors.lastname: de Albuquerque
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: nyzfd_@hotmail.com.
authors.forename: Maria Neyze Martins
authors.initials: MNM
authors.lastname: Fernandes
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: brunoskarllet2@gmail.com.
authors.forename: Bruno Anderson Fernandes
authors.initials: BAF
authors.lastname: da Silva
authors.affiliation: Graduate Program in Health Sciences, Federal University of Sergipe, Aracaju, CEP 49100-000, Sergipe, Brazil. Electronic address: lucindojr@gmail.com.
authors.forename: Lucindo José
authors.initials: LJ
authors.lastname: Quintans Júnior
authors.affiliation: Graduate Program in Health Sciences, Federal University of Sergipe, Aracaju, CEP 49100-000, Sergipe, Brazil. Electronic address: adriasa2001@yahoo.com.br.
authors.forename: Adriano Antunes de Sousa
authors.initials: AAS
authors.lastname: Araújo
authors.affiliation: Graduate Program in Health Sciences, Federal University of Sergipe, Aracaju, CEP 49100-000, Sergipe, Brazil. Electronic address: paulamenezes_16@yahoo.com.br.
authors.forename: Paula Dos Passos
authors.initials: PDP
authors.lastname: Menezes
authors.affiliation: Graduate Program in Health Sciences, Federal University of Sergipe, Aracaju, CEP 49100-000, Sergipe, Brazil. Electronic address: paulanunes_se@yahoo.com.br.
authors.forename: Paula Santos
authors.initials: PS
authors.lastname: Nunes
authors.affiliation: Graduate Program in Health Sciences, Federal University of Sergipe, Aracaju, CEP 49100-000, Sergipe, Brazil. Electronic address: Matos_isabella@hotmail.com.
authors.forename: Isabella Gonçalves
authors.initials: IG
authors.lastname: Matos
authors.affiliation: Microbiology and Biology Molecular Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: hdmcoutinho@gmail.com.
authors.forename: Henrique Douglas Melo
authors.initials: HDM
authors.lastname: Coutinho
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Pernambuco, CEP 50670-901, Recife, PE, Brazil. Electronic address: almirgw.wanderley@gmail.com.
authors.forename: Almir
authors.initials: A
authors.lastname: Goncalves Wanderley
authors.affiliation: Pharmacology and Molecular Chemistry Laboratory, Department of Chemical Biology, Regional University of Cariri, CEP 63105-000, Crato, Ceara, Brazil. Electronic address: irwin.alencar@urca.br.
authors.forename: Irwin Rose Alencar
authors.initials: IRA
authors.lastname: de Menezes
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D001938:Brazil; D004487:Edema / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D018649:Ocimum basilicum / Q000737:chemistry*; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D010936:Plant Extracts; D047392:beta-Cyclodextrins
keywords: Anti-inflammatory activity; Arachidonic acid; Ocimum basilicum; β-cyclodextrin