title: Experimental burns: Comparison between silver sulfadiazine and photobiomodulation.
abstract: OBJECTIVE
  To analyze morphological characteristics and organization of the collagen fibers of third degree burns from scalding compared to laser therapy and silver sulfadiazine, the latter considered as the gold standard.
  
  
  METHODS
  Were selected 12 animals (Rattus norvegicus) also divided into three groups (control group [CG] - untreated burns; sulfadiazine group [SG] - burns were treated with silver sulfadiazine at 1%; laser group [LG] - burns were treated with photobiomodulation). The scald burns were carried out by using PVC mold, and the material collected on the 14th day after burn was prepared for morphological and optical retardation analysis for evaluation of inflammatory infiltrates and collagen organization, respectively.
  
  
  RESULTS
  On the 14th day, the laser and sulfadiazine groups had mild inflammatory response, while the control group showed an intense inflammatory process, with statistical significance between laser and control groups, but not between sulfadiazine and control groups. Laser and sulfadiazine groups no longer had granulation tissue, opposite to what was seen in the control group. The presence of hair follicles and ulcer did not significantly differ between groups. The optical retardation of collagen fibers was higher in sulfadiazine group, followed by laser and control groups. As for systemic effect, we were able to identify it by simply analyzing the presence or absence of granulation tissue.
  
  
  CONCLUSIONS
  Morphologically, the laser or silver sulfadiazine treatments were similar and both provided better organization of collagen fibers in relation to the untreated group. However, the sulfadiazine group modulated the deposition of collagen fibers more efficiently than the laser group.
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Mariana Teixeira
authors.initials: MT
authors.lastname: Gomes
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Gabriela Russo Soeiro
authors.initials: GR
authors.lastname: Campos
authors.affiliation: Uninove, São Paulo, SP, Brazil.
authors.forename: Natália
authors.initials: N
authors.lastname: Piccolo
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Cristiane Miranda
authors.initials: CM
authors.lastname: França
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Guelton Hirano
authors.initials: GH
authors.lastname: Guedes
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Fabio
authors.initials: F
authors.lastname: Lopes
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Renata A
authors.initials: RA
authors.lastname: Belotto
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Christiane
authors.initials: C
authors.lastname: Pavani
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Rafael do Nascimento de
authors.initials: RD
authors.lastname: Lima
authors.affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, São Paulo, SP, Brazil.
authors.forename: Daniela de Fátima Teixeira da
authors.initials: DF
authors.lastname: Silva
mesh_terms: D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy*; D003094:Collagen / Q000187:drug effects / Q000528:radiation effects; D004195:Disease Models, Animal; D005260:Female; D028022:Low-Level Light Therapy / Q000379:methods*; D051381:Rats; D012837:Silver Sulfadiazine / Q000008:administration & dosage*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology
chemical_list: D003094:Collagen; D012837:Silver Sulfadiazine