title: Origanum MajoranumExtract Modulates Gene Expression, Hepatic and Renal Changes in a Rat Model of Type 2 Diabetes.
abstract: The present study was conducted to test the effect ofOriganum MajoranumExtract(OME) of leaves on alterations induced in a model of type 2 diabetic rats. Adult male Wistar rats were fed high fat diet for 3 weeks and injected a single dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetic rats. Diabetic rats were given aqueous extract of OME in a dose of 20 mg/kg orally for 3 weeks. Changes in lipid profiles, glucose, insulin, expression of some genes related to glucose metabolism and histopathological changes in liver and kidney were examined. Administration of OME improved and normalized dyslipidemia recorded in type 2 diabetic rats together with reduction in glucose and insulin levels. OME induced up-regulation in gene expression of glucose [adiponectin and glucose transporter-2 (GLUT-2)] and lipid metabolism [lipoprotein lipase (LPL)]. Moreover, OME normalized histopathological changes occurred in liver and kidney of diabetic rats. OME decreased lipids accumulation in liver and kidney and increased regeneration of hepatic parenchyma and restored normal renal architecture with disappearance of fat droplets. In conclusion, OME improved dyslipidemia associated with type 2 diabetes through regulation of genes related to glucose and lipid metabolism.
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt. ; Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia.
authors.forename: Mohamed Mohamed
authors.initials: MM
authors.lastname: Soliman
authors.affiliation: Departments of Pathology and Physiology.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdo Nassan
authors.affiliation: Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia. ; Faculty of Veterinary Medicine, Zagazig University, Egypt.
authors.forename: Tamer Ahmed
authors.initials: TA
authors.lastname: Ismail
keywords: Dyslipidemia; Gene expression; Hepatic and renal histopathology; Origanum extract; Type 2 diabetes