title: Cytotoxicity of arsenic trioxide in single leukemia cells by time-resolved ICP-MS together with lanthanide tags.
abstract: A novel strategy using cisplatin as a viability dye together with conjugating lanthanide tags to marker proteins was developed to examine As2O3cytotoxicity in single NB4/HL60 cells via ICP-MS. A positive correlation among intracellular arsenic contents, the ratios of apoptosis and death cells provides a basis for the design of more effective arsenic drugs.
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
authors.forename: Hongzhe
authors.identifier: http://orcid.org/0000-0001-6697-6899
authors.initials: H
authors.lastname: Sun
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry / Q000494:pharmacology*; D007938:Leukemia / Q000473:pathology*; D013058:Mass Spectrometry; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D059010:Single-Cell Analysis; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D028581:Lanthanoid Series Elements; D010087:Oxides; D002945:Cisplatin; D000077237:Arsenic Trioxide