title: 20-Hydroxyecdysone from Dacrycarpus imbricatus bark inhibits the proliferation of acute myeloid leukemia cells.
abstract: OBJECTIVE
  To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus (Blume) de Laub.
  
  
  METHODS
  Column chromatography was used for isolation of compounds from plant material. The structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques, including HSQC, HMBC, NOE-difference experiments. The isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia (AML) and OCI-AML cells.
  
  
  RESULTS
  Compound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column chromatography. Its chemical structure was identified as 20-hydroxyecdysone (20HE), a cholestane-type ecdysteroid, by a combination of mass spectrometry and nuclear magnetic resonance spectrometric analyses. Our goal was to test the anti-proliferative activity of 20HE using the OCI-AML cell line. 20HE significantly decreased OCI cell number at a concentration of 1 mg/mL, whereas lower concentrations were ineffective. Moreover, this decrease was due to partial blockage of the G1/S phase of the cell cycle, with a reduction of cells in the G2M phase, not due to increased apoptosis.
  
  
  CONCLUSIONS
  This indicates that 20HE significantly decreases the number of cells in the G1/S phase of the cell cycle in human AML cells. This is the first time that the anti-proliferative activity of 20HE against a human tumor cell line has been reported.
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Ha Noi, Viet Nam.
authors.forename: Trinh Thi
authors.initials: TT
authors.lastname: Thuy
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Ha Noi, Viet Nam.
authors.forename: Nguyen Thanh
authors.initials: NT
authors.lastname: Tam
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Ha Noi, Viet Nam.
authors.forename: Nguyen Thi Hoang
authors.initials: NT
authors.lastname: Anh
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Ha Noi, Viet Nam.
authors.forename: Dang Viet
authors.initials: DV
authors.lastname: Hau
authors.affiliation: Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Ha Noi, Viet Nam.
authors.forename: Dinh Thi
authors.initials: DT
authors.lastname: Phong
authors.affiliation: Hue University's College of Educations, 34 Le Loi, Hue City, Viet Nam.
authors.forename: Le Quoc
authors.initials: LQ
authors.lastname: Thang
authors.affiliation: Section of Pharmacology, Department of Medicine, University of Perugia, Piazzale Severi, S. Andrea delle Fratte, 06132 Perugia, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Adorisio
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Ha Noi, Viet Nam.
authors.forename: Tran
authors.initials: T
authors.lastname: Van Sung
authors.affiliation: Section of Pharmacology, Department of Medicine, University of Perugia, Piazzale Severi, S. Andrea delle Fratte, 06132 Perugia, Italy. Electronic address: domenico.delfino@unipg.it.
authors.forename: Domenico V
authors.initials: DV
authors.lastname: Delfino
keywords: 20-Hydroxyecdysone; Acute myeloid leukemia; Dacrycarpus imbricatus; Ecdysteroid; Proliferation