title: Multicenter evaluation of the revised RIDA® QUICK test (N1402) for rapid detection of norovirus in a diagnostic laboratory setting.
abstract: The updated RIDA® QUICK (N1402) immunochromatographic assay (R-Biopharm) for detection of norovirus was evaluated during a prospective, multicenter study using 771 stool samples from patients with gastroenteritis. Compared to real-time reverse transcriptase polymerase chain reaction (RT-rtPCR) as gold standard, the RIDA® QUICK had an overall sensitivity of 72.8% (91/125) and a specificity of 99.5% (640/643). Genotype analysis of the polymerase (ORF1) and capsid (ORF2) region of the genome indicated that the RIDA® QUICK assay could detect a broad range of genotypes including new variants (15 of 125 positive samples) which were detected by an in-house SYBR®Green RT-rtPCR, but not by the RIDA® GENE PCR PG1415 (R-Biopharm) and mostly not by the RIDA® GENE PCR PG1405 and the Xpert® Norovirus assay (Cepheid). The RIDA® QUICK can be used to reliably confirm norovirus in stool samples, but a negative result does not definitively exclude the presence of norovirus.
authors.affiliation: Clinical Laboratory of Microbiology, OLV Hospital Aalst, Moorselbaan 164, 9300, Aalst, Belgium; Clinical Laboratory of Microbiology, Jan Yperman Hospital, Briekestraat 12, 8900, Ieper, Belgium. Electronic address: Stijn.Jonckheere@yperman.net.
authors.forename: Stijn
authors.initials: S
authors.lastname: Jonckheere
authors.affiliation: National Reference Center for Norovirus, WIV-ISP, Site Elsene, Juliette Wytsmansstraat 14, 1050, Brussel, Belgium.
authors.forename: Nadine
authors.initials: N
authors.lastname: Botteldoorn
authors.affiliation: Clinical Laboratory of Microbiology, Jan Yperman Hospital, Briekestraat 12, 8900, Ieper, Belgium.
authors.forename: Patricia
authors.initials: P
authors.lastname: Vandecandelaere
authors.affiliation: Clinical Laboratory of Microbiology, Imelda Hospital, Imeldalaan 9, 2820, Bonheiden, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Frans
authors.affiliation: Clinical Laboratory of Microbiology, HH Hospital Lier, Mechelsestraat 24, 2500, Lier, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Laffut
authors.affiliation: Clinical Laboratory of Microbiology, Hospital Oost-, Limburg, Schiepse Bos 6, 3600, Genk, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Coppens
authors.affiliation: Clinical Laboratory of Microbiology, OLV Hospital Aalst, Moorselbaan 164, 9300, Aalst, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Vankeerberghen
authors.affiliation: Clinical Laboratory of Microbiology, OLV Hospital Aalst, Moorselbaan 164, 9300, Aalst, Belgium.
authors.forename: Hans
authors.initials: H
authors.lastname: De Beenhouwer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017250:Caliciviridae Infections / Q000175:diagnosis*; D002648:Child; D002846:Chromatography, Affinity / Q000379:methods*; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D029322:Norovirus / Q000302:isolation & purification*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors; D055815:Young Adult
keywords: Antigen test; Norovirus; RIDA® gene; RIDA® quick; Xpert® norovirus