title: A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique.
abstract: Koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. In the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. This approach aimed at detecting the low-abundant bacteria in the koumiss. Briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. Then, three diluted bacterial suspensions were randomly picked for further study. By analyzing 30 diluted koumiss suspensions, a total of 24 bacterial species were identified. In addition to the previously reported koumiss-associated species, such asLactobacillus(L.)helveticus. Lactococcus lactis. L. buchneri, L. kefiranofaciens, andAcetobacter pasteurianus, we successfully detected three low-abundant taxa in the samples, namelyL. otakiensis. Streptococcus macedonicus, andRuminococcus torques. The functional koumiss metagenomes carried putative genes that relate to lactose metabolism and synthesis of typical flavor compounds. Our study would encourage the use of modern metagenomics to discover novel species of bacteria that could be useful in food industries.
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Yao
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Qiangchuan
authors.initials: Q
authors.lastname: Hou
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Hui
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Lai-Yu
authors.initials: LY
authors.lastname: Kwok
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Bilige
authors.initials: B
authors.lastname: Menghe
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Tiansong
authors.initials: T
authors.lastname: Sun
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Heping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University Hohhot, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhang
keywords: bacterial diversity; koumiss; low-abundant taxa; metagenomics; single-cell amplification