title: Purification of Lactobacillus acidophilus surface-layer protein and its immunomodulatory effects on RAW264.7 cells.
abstract: BACKGROUND
  Surface-layer proteins (SLP) have been found in the outermost layer of the cell wall in many types of lactobacillus are considered to be an important factor with respect to intestinal immunity.
  
  
  RESULTS
  The present study compared the effects of SLP extracted by different concentrations of LiCl and carbamide, and subsequently identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, circular dichroism and differential scanning calorimetry. Furthermore, RAW 264.7 cells were used to evaluate the immunomodulatory effects of SLP. SLP were derived from Lactobacillus acidophilus CICC6074 with a molecular weight of 46 kDa, and consisted of 16.9% α-helix, 42.3% β-sheet, 20.8% β-turns and 22.5% random coils. SLP promoted NO secretion and higher quantities of NO were produced as the SLP concentrations increased. SLP concentrations over 50 µg mL-1significantly decreased the amount of tumor necrosis factor-α secreted by RAW264.7 cells.
  
  
  CONCLUSIONS
  SLP can trigger immunomodulatory effects in RAW 264.7 cells. This provides crucial information that will enable the further use of L. acidophilus in food, medicine and other products. © 2017 Society of Chemical Industry.
authors.affiliation: Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, PR China.
authors.forename: Mengting
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, PR China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, PR China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Xun
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo, Zhejiang, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo, Zhejiang, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, PR China.|Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo, Zhejiang, PR China.
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology*; D007155:Immunologic Factors / Q000302:isolation & purification* / Q000494:pharmacology*; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D000067996:RAW 264.7 Cells
chemical_list: D001426:Bacterial Proteins; D007155:Immunologic Factors; D008562:Membrane Glycoproteins; C055764:S-layer proteins
keywords: Lactobacillus acidophilus; immunomodulatory capacity; purification; surface-layer protein