title: Interferon-α-induced CD100 on naïve CD8+T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction.
abstract: Objectives We investigated the effects of CD100 on naïve CD8+T cells during hepatitis C virus (HCV) infection after interferon-α (IFN-α) therapy to clarify the mechanism underlying the effect of IFN-α in enhancing the antiviral response. Methods The CD100 molecules on subsets of CD8+T cells were analysed with flow cytometry. The effects of CD100-overexpressing naïve CD8+T cells were determined with ELISAs and an MTT cytotoxicity assay. The role of CD100-CD72 signal transduction was analysed with a neutralization and transwell assays. Results HCV infection reduced CD100 expression on CD8+T cells, whereas IFN-α treatment significantly increased CD100 expression on naïve CD8+T cells. The increased CD100 interacted with the CD72 receptor and enhanced PBMC cytokine secretion (IFN-γ and tumour necrosis factor-α) and cytotoxicity. Conclusions IFN-α-induced CD100 on naïve CD8+T cells promotes PBMC cytokine secretion and cytotoxicity through CD100-CD72 signalling during HCV infection.
authors.affiliation: 2 First Affiliated Hospital, School of Medicine, Shihezi University, Xinjiang, China.
authors.forename: Bing Jie
authors.initials: BJ
authors.lastname: Li
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Yong Hong
authors.initials: YH
authors.lastname: Guo
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Pei Xin
authors.initials: PX
authors.lastname: Zhang
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Jie Ru
authors.initials: JR
authors.lastname: Zhao
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Jin Ge
authors.initials: JG
authors.lastname: Li
authors.affiliation: 2 First Affiliated Hospital, School of Medicine, Shihezi University, Xinjiang, China.
authors.forename: Wei Ze
authors.initials: WZ
authors.lastname: Zuo
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Fan
authors.affiliation: 1 Department of Infectious Diseases and Center of Liver Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Zhan Sheng
authors.initials: ZS
authors.lastname: Jia
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics* / Q000276:immunology; D000998:Antiviral Agents / Q000627:therapeutic use*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D016022:Case-Control Studies; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D005260:Female; D005786:Gene Expression Regulation; D016174:Hepacivirus / Q000187:drug effects / Q000254:growth & development / Q000276:immunology; D006526:Hepatitis C / Q000188:drug therapy* / Q000276:immunology / Q000821:virology; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use*; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000821:virology; D008297:Male; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000627:therapeutic use*; D061251:Primary Cell Culture; D012367:RNA, Viral / Q000187:drug effects; D011994:Recombinant Proteins / Q000627:therapeutic use; D039961:Semaphorins / Q000235:genetics* / Q000276:immunology; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000998:Antiviral Agents; C093224:CD100 antigen; C064228:CD72 protein, human; D016898:Interferon-alpha; D012367:RNA, Viral; D011994:Recombinant Proteins; D039961:Semaphorins; D014409:Tumor Necrosis Factor-alpha; D011092:Polyethylene Glycols; D007371:Interferon-gamma; C100416:peginterferon alfa-2a
keywords: CD100; IFN-α therapy; hepatitis C; naïve CD8+ T cell