title: Effects of duodenal-jejunal bypass on structure of diaphragm in western diet obese rats.
abstract: To evaluate the effects of duodenal-jejunal bypass (DJB) on the diaphragm muscle of obese rats fed on a western diet (WD) .
  Eighteen male Wistar rats were fed a standard rodent chow diet (CTL group) or WD ad libitum. After 10 weeks, WD rats were submitted to sham (WD SHAM) or duodenal-jejunal bypass (WD DJB). The structure, ultrastructure, collagen content and the morphometry of the neuromuscular junctions (NMJs) were analyzed two months after surgery.
  WD SHAM rats displayed an increase in body weight, the Lee index and retroperitoneal and peri-epididymal fat pads compared to the CTL group. DJB did not alter these parameters. The muscle fiber structure and NMJs were similar in the WD SHAM and CTL groups. However, the WD SHAM group showed alterations in the fiber ultrastructure, such as loosely arranged myofibrils and Z line disorganization. In addition, WD SHAM animals presented a considerable amount of lipid droplets and a reduction in the percentage of collagen compared to the CTL group. DJB did not affect the structure or ultrastructure of the muscle fibers or the NMJs in the diaphragm of the WD DJB animals.
  Duodenal-jejunal bypass did not improve the alterations observed in the diaphragm of western diet obese-rats.
authors.affiliation: Fellow Master degree, Postgraduate Program in Biosciences and Health, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel-PR, Brazil. Scholarship research from CAPES and Araucaria Foundation. Acquisition and interpretation of data, statistical analysis.
authors.forename: Bruna Hart
authors.initials: BH
authors.lastname: Ulsenheimer
authors.affiliation: Fellow Master degree, Postgraduate Program in Biosciences and Health, UNIOESTE, Cascavel-PR, Brazil. Technical procedures.
authors.forename: Heloisa Deola
authors.initials: HD
authors.lastname: Confortim
authors.affiliation: Fellow Master degree, Postgraduate Program in Biosciences and Health, UNIOESTE, Cascavel-PR, Brazil. Technical procedures.
authors.forename: Léslie Cazetta
authors.initials: LC
authors.lastname: Jeronimo
authors.affiliation: PhD, Assistant Professor, Center of Medical and Pharmaceutical Sciences, UNIOESTE, Cascavel-PR, Brazil. Conception, design, intellectual and scientific content of the study; manuscript writing.
authors.forename: Lígia Aline
authors.initials: LA
authors.lastname: Centenaro
authors.affiliation: PhD, Assistant Professor, Center for Biological Sciences and Health, UNIOESTE, Cascavel-PR, Brazil. Statistical analysis.
authors.forename: Ana Tereza Bittencourt
authors.initials: AT
authors.lastname: Guimarães
authors.affiliation: PhD, Assistant Professor, Human Physiology Division, Center for Biological Sciences and Health, UNIOESTE, Cascavel-PR, Brazil. Conception, intellectual and scientific content of the study; manuscript writing.
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Bonfleur
authors.affiliation: PhD, Assistant Professor, Human Physiology Division, Center for Biological Sciences and Health, UNIOESTE, Cascavel-PR Brazil. Intellectual and scientific content of the study.
authors.forename: Sandra Lucinei
authors.initials: SL
authors.lastname: Balbo
authors.affiliation: PhD, Assistant Professor, Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Transmission electron microscope analysis.
authors.forename: Selma Maria Michelin
authors.initials: SM
authors.lastname: Matheus
authors.affiliation: PhD, Assistant Professor, Center of Medical and Pharmaceutical Sciences, UNIOESTE, Cascavel-PR, Brazil. Conception, design, intellectual and scientific content of the study; technical procedures.
authors.forename: Marcia Miranda
authors.initials: MM
authors.lastname: Torrejais
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003964:Diaphragm / Q000648:ultrastructure*; D066273:Diet, Western; D004386:Duodenum / Q000601:surgery*; D007583:Jejunum / Q000601:surgery*; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000648:ultrastructure*; D009765:Obesity / Q000378:metabolism / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar