title: Effect of Platelet-rich Fibrin on Odontoblastic Differentiation in Human Dental Pulp Cells Exposed to Lipopolysaccharide.
abstract: BACKGROUND
  Platelet-rich fibrin (PRF), as an autologous fibrin matrix, is known to contain platelets, leukocytes, and growth factors to control inflammation and to facilitate the healing process. The purpose of this study was to investigate the effects of PRF on odontoblastic differentiation in human dental pulp cells (HDPCs) treated with lipopolysaccharide (LPS).
  
  
  METHODS
  Gene expression of inflammatory cytokines and adhesion molecules on the HDPCs cultured with or without LPS and PRF extract (PRFe) were evaluated by reverse-transcription polymerase chain reaction and Western blot analysis. In addition, odontoblastic differentiation was determined by measuring alkaline phosphatase (ALP) activity using ALP staining, the expression of odontogenesis-related genes, and the extent of mineralization using alizarin red S staining.
  
  
  RESULTS
  Treatment with PRFe significantly attenuated the LPS-stimulated expression of interleukin (IL)-1β, IL-6, and IL-8 in HDPCs. In addition, PRFe inhibited the up-regulation of vascular cell adhesion molecule 1 and the production of intracellular adhesion molecule 1 in HDPCs exposed to LPS. Expression of dentin sialophosphoprotein and dentin matrix acidic phosphoprotein 1, ALP activity, and mineralization were enhanced by PRFe in LPS-treated HDPCs.
  
  
  CONCLUSIONS
  These results suggest that PRF has effects associated not only with inhibition of inflammation in HDPCs exposed to LPS but also stimulation of odontoblastic differentiation.
authors.affiliation: Department of Pediatric Dentistry, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea.
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Oral Physiology, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea; Research Center for Biomineralization Disorder, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea.
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Woo
authors.affiliation: Department of Pediatric Dentistry, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea.
authors.forename: Nam-Ki
authors.initials: NK
authors.lastname: Choi
authors.affiliation: Department of Oral Physiology, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea; Research Center for Biomineralization Disorder, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea.
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Department of Pediatric Dentistry, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea. Electronic address: gracekim@jnu.ac.kr.
authors.forename: Seon-Mi
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Oral Physiology, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea; Research Center for Biomineralization Disorder, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea. Electronic address: jjy@jnu.ac.kr.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Jung
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D008070:Lipopolysaccharides / Q000494:pharmacology; D009804:Odontoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology; D010750:Phosphoproteins / Q000378:metabolism; D000073183:Platelet-Rich Fibrin; D015854:Up-Regulation
chemical_list: D016207:Cytokines; C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; D008070:Lipopolysaccharides; D010750:Phosphoproteins; D000469:Alkaline Phosphatase
keywords: Human dental pulp cells; lipopolysaccharide; odontoblastic differentiation; platelet-rich fibrin