title: Characterization of Plant Cell Wall Damage-Associated Molecular Patterns Regulating Immune Responses.
abstract: The plant cell wall is one of the first defensive barriers that pathogens need to overcome to successfully colonize plant tissues. Plant cell wall is considered a dynamic structure that regulates both constitutive and inducible defense mechanisms. The wall is a potential source of a diverse set of Damage-Associated Molecular Patterns (DAMPs), which are signalling molecules that trigger immune responses. However, just a few active wall ligands, such as oligogalacturonic acids (OGs), have been characterized so far. To identify additional wall-derived DAMPs, we obtained different plant wall fractions and tested their capacity to trigger immune responses using a calcium read-out system. To characterize the active DAMPs structures present in these fractions, we applied Glycome Profiling, a technology that uses a large and diverse set of specific monoclonal antibodies against wall carbohydrate ligands. The methods describe here can be used in combination with other biochemical approaches to identify and purify new plant cell wall DAMPs.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, 28223, Pozuelo de Alarcón (Madrid), Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Bacete
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, 28223, Pozuelo de Alarcón (Madrid), Spain.
authors.forename: Hugo
authors.initials: H
authors.lastname: Mélida
authors.affiliation: Complex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA, 30602, USA.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Pattathil
authors.affiliation: Complex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA, 30602, USA.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Hahn
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, 28223, Pozuelo de Alarcón (Madrid), Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molina
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo UPM, 28223, Pozuelo de Alarcón (Madrid), Spain. eva.miedes@upm.es.
authors.forename: Eva
authors.initials: E
authors.lastname: Miedes
mesh_terms: D017360:Arabidopsis / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000302:isolation & purification; D002241:Carbohydrates / Q000302:isolation & purification; D002473:Cell Wall / Q000276:immunology*; D018506:Gene Expression Regulation, Plant; D054794:Glycomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D002241:Carbohydrates
keywords: Arabidopsis; Cell wall; Hemicellulose; Immunity; Pectin