title: The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies.
abstract: High throughput genomic assays empower us to study the entire human genome in short time with reasonable cost. Formalin fixed-paraffin-embedded (FFPE) tissue processing remains the most economical approach for longitudinal tissue specimen storage. Therefore, the ability to apply high throughput genomic applications to FFPE specimens can expand clinical assays and discovery. Many studies have measured the accuracy and repeatability of data generated from FFPE specimens using high throughput genomic assays. Together, these studies demonstrate feasibility and provide crucial guidance for future studies using FFPE specimens. Here, we summarize the findings of these studies and discuss the limitations of high throughput data generated from FFPE specimens across several platforms that include microarray, high throughput sequencing, and NanoString.
authors.affiliation: Department of Cancer Biology, Vanderbilt University, Nashville, TN, USA.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.
authors.forename: Brian D
authors.identifier: 0000-0002-4269-2274
authors.initials: BD
authors.lastname: Lehmann
authors.affiliation: Department of Biostatistics, Vanderbilt University, Nashville, TN, USA.
authors.forename: Yu
authors.identifier: 0000-0003-2086-9670
authors.initials: Y
authors.lastname: Shyr
authors.affiliation: Department of Cancer Biology, Vanderbilt University, Nashville, TN, USA.
authors.forename: Yan
authors.identifier: 0000-0001-5252-3960
authors.initials: Y
authors.lastname: Guo