title: Expression and characterization of the major capsid protein derived from a GII.6 norovirus strain isolated in China.
abstract: The objective of this study was to express and characterize the major capsid protein (VP1) of a GII.6 Norovirus (NoV)strain isolated in China. The newly identified GII.6 NoV strain was isolated from a five-year old boy presenting acute gastroenteritis. The genome of the GII.6 strain was 7550 nucleotides in length, excluding the poly-adenylation tail. Multiple sequence alignment and phylogenetic analysis based on deduced VP1 amino acid sequences from different genotypes indicated close relationship between GII.3 and GII.6 NoVs, as demonstrated by the presence of a short sequence insertion in the P2 domain and clustering in the same subgroup. Expression of GII.6 VP1 led to assembly of virus like particles (VLPs). In vitro VLP-salivary histo-blood group antigens (HBGAs) binding assay demonstrated wide-spectrum binding activities of assembled VLPs to blood type A, B, AB and O salivary HBGAs with highest binding capacity to type A salivary HBGAs and lowest to type AB and O salivary HBGAs. In vitro VLP-salivary HBGAs binding blockade assay indicated absence of cross-blocking effects for hyperimmune sera produced against different genotypes. In conclusion, our results suggest a rational VLPs-based multivalent NoV vaccine should contain capsid proteins of a GII.6 strain.
authors.affiliation: The Sixth People's Hospital of Zhengzhou, Zhengzhou, PR China. Electronic address: 1246105971@qq.com.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Huo
authors.affiliation: The Sixth People's Hospital of Zhengzhou, Zhengzhou, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zheng
authors.affiliation: The Sixth People's Hospital of Zhengzhou, Zhengzhou, PR China.
authors.forename: Xuhui
authors.initials: X
authors.lastname: Chen
authors.affiliation: Children's Hospital of Zhengzhou, Zhengzhou, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ge
authors.affiliation: The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001789:Blood Group Antigens / Q000378:metabolism; D017250:Caliciviridae Infections / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D002675:Child, Preschool; D002681:China; D003001:Cloning, Molecular; D005759:Gastroenteritis / Q000821:virology; D015870:Gene Expression; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008297:Male; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D011485:Protein Binding; D055503:Protein Multimerization; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D022701:Virosomes / Q000378:metabolism
chemical_list: D001789:Blood Group Antigens; D036022:Capsid Proteins; D022701:Virosomes
keywords: Capsid proteins; Cross-blocking antibody; Histo-blood group antigens; Noroviruses; Virus like particles