title: Gingival crevicular fluid levels of monocyte chemoattractant protein-1 in patients with aggressive periodontitis.
abstract: OBJECTIVE
  The purpose of this study was to investigate the gingival crevicular fluid (GCF) levels of monocyte chemoattractant protein (MCP)-1 in aggressive periodontitis (AgP) and whether GCF MCP-1 levels differ among localized (L) AgP and generalized (G) AgP.
  
  
  METHODS
  A total of 160 subjects including 80 AgP and 80 age- and gender-matched periodontally healthy (H) controls were recruited in this cross-sectional study (NCT02927704). GCF samples were collected from 160 patients including 50 LAgP, 30 GAgP, and 80 H. Volume of GCF was measured by Periotron 8000®, and enzyme-linked immunosorbent assay was used to assess MCP-1 levels.
  
  
  RESULTS
  Compared to H controls, all clinical parameters and total amounts (pg 30 s-1) of MCP-1 were significantly higher in subjects with LAgP and GAgP (P < 0.05). Although concentrations of GCF MCP-1 did not differ between LAgP and GAgP (P > 0.05), total amounts of MCP-1 were higher in GAgP than LAgP (P < 0.05).
  
  
  CONCLUSIONS
  It can be concluded that the total amount of MCP-1 level in GCF may be a potential determinant in AgP subjects. Increased MCP-1 levels in line with the degree of periodontal destruction in GAgP patients reveal that MCP-1 can be used to understand the disease pathogenesis of LAgP and GAgP.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Abant Izzet Baysal University, Bolu, Turkey.
authors.forename: S
authors.identifier: http://orcid.org/0000-0001-6100-322X
authors.initials: S
authors.lastname: Gunpinar
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Baskent University, Ankara, Turkey.
authors.forename: N O
authors.initials: NO
authors.lastname: Alptekin
authors.affiliation: Research Center of Dental Faculty, Selcuk University, Konya, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Dundar
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000378:metabolism*; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005260:Female; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D008297:Male; D055815:Young Adult
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2
keywords: aggressive periodontitis; enzyme-linked immunosorbent assay; gingival crevicular fluid; monocyte chemoattractant protein-1