title: Akirin2 promotes slow myosin heavy chain expression by CaN/NFATc1 signaling in porcine skeletal muscle satellite cells.
abstract: The objective of this study was to evaluate the effect of Akirin2 on slow myosin heavy chain (slow MyHC, MyHC I) gene expression and its molecular mechanisms. In this study, we showed that the protein expression of Akirin2 in pig slow oxidative Psoas major muscle is higher than that in fast glycolytic tibialis anterior muscle, suggesting that Akirin2 may play a role in myofiber typing. Knockdown of Akirin2 decreased the MyHC I expression and the calcineurin (CaN) activity, and also decreased the expressions of NFATc1 and MCIP1.4. Conversely, overexpression of Akirin2 got the opposite results. Furthermore, inhibition of CaN or knockdown of NFATc1 attenuated Akirin2 overexpression-induced upregulation of MyHC I. Together, these results demonstrate that Akirin2 promotes MyHC I expression via CaN/NFATc1 signaling pathway in porcine skeletal muscle satellite cells.
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
authors.forename: Yanliu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D018995:Myosin Heavy Chains / Q000235:genetics*; D050778:NFATC Transcription Factors / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*; D015398:Signal Transduction; D013552:Swine
chemical_list: D050778:NFATC Transcription Factors; D028861:Nuclear Pore Complex Proteins; D012097:Repressor Proteins; D018995:Myosin Heavy Chains
keywords: Akirin2; CaN/NFATc1; porcine skeletal muscle satellite cells; slow myosin heavy chain