title: Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting.
abstract: Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.
authors.affiliation: Institute of Eye Diseases, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Novikov
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Vakhrushev
authors.affiliation: Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Antonov
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
authors.affiliation: Institute of Eye Diseases, Moscow, Russia. kletkagb@gmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Subbot
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects; D005898:Glass / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009354:Neodymium / Q000737:chemistry*; D010012:Osteogenesis; D010969:Plastics / Q000737:chemistry / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology; D061251:Primary Cell Culture; D013194:Staining and Labeling / Q000379:methods; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014094:Tooth, Deciduous / Q000166:cytology / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D003287:Contrast Media; D010969:Plastics; D011091:Polyesters; D011137:Polystyrenes; C053739:neodymium chloride; D009354:Neodymium; C033616:poly(lactide)
keywords: lanthanide; mesenchymal stromal cells; scanning electron microscopy; tissue engineering