title: Function of heterochromatin protein 1 during DNA repair.
abstract: This review focuses on the function of heterochromatin protein HP1 in response to DNA damage. We specifically outline the regulatory mechanisms in which HP1 and its interacting partners are involved. HP1 protein subtypes (HP1α, HP1β, and HP1γ) are the main components of constitutive heterochromatin, and HP1α and HP1β in particular are responsible for heterochromatin maintenance. The recruitment of these proteins to DNA lesions is also important from the perspective of proper DNA repair mechanisms. For example, HP1α is necessary for the binding of the main DNA damage-related protein 53BP1 at DNA repair foci, which are positive not only for the HP1α protein but also for the RAD51 protein, a component of DNA repair machinery. The HP1β protein also appears in monomeric form in DNA lesions together with the evolutionarily well-conserved protein called proliferating cell nuclear antigen (PCNA). The role of HP1 in DNA lesions is also mediated via the Kap1 transcription repressor. Taken together, these results indicate that the function of HP1 after DNA injury depends strongly on the kinetics of other DNA repair-related factors and their post-translational modifications, such as the phosphorylation of Kap-1.
authors.affiliation: Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic. bartova@ibp.cz.
authors.forename: Eva
authors.initials: E
authors.lastname: Bártová
authors.affiliation: Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Malyšková
authors.affiliation: Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic.
authors.forename: Denisa
authors.initials: D
authors.lastname: Komůrková
authors.affiliation: Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic.
authors.forename: Soňa
authors.initials: S
authors.lastname: Legartová
authors.affiliation: Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Suchánková
authors.affiliation: Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Krejčí
authors.affiliation: Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kozubek
mesh_terms: D002843:Chromatin / Q000378:metabolism; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins / Q000378:metabolism*; D000076183:Tripartite Motif-Containing Protein 28
chemical_list: C000715593:CBX1 protein, human; C000715594:CBX5 protein, human; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D018809:Proliferating Cell Nuclear Antigen; D012097:Repressor Proteins; D000090266:Chromobox Protein Homolog 5; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28
keywords: DNA damage response; DNA repair; HP1 protein