title: Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion.
abstract: Zebrafish gastrulation and particularly epiboly that involves coordinated movements of several cell layers is a dynamic process for which regulators remain to be identified. We show here that Flotillin 1 and 2, ubiquitous and highly conserved proteins, are required for epiboly. Flotillins knockdown compromised embryo survival, strongly delayed epiboly and impaired deep cell radial intercalation and directed collective migration without affecting enveloping layer cell movement. At the molecular level, we identified that Flotillins are required for the formation of E-cadherin-mediated cell-cell junctions. These results provide the first in vivo evidence that Flotillins regulate E-cadherin-mediated cell-cell junctions to allow epiboly progression.
authors.affiliation: CRBM, CNRS, UMR 5237, Université de Montpellier, Equipe Labellisée Ligue contre le Cancer, Montpellier, 34293, France.|MRI-CRBM Imaging facility, CNRS, UMR 5237, Université de Montpellier, Montpellier, 34293, France.|Laboratório de Diferenciação Muscular e Citoesqueleto, Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21949-590, Brazil.
authors.forename: Eduardo A Rios
authors.initials: EAR
authors.lastname: Morris
authors.affiliation: CRBM, CNRS, UMR 5237, Université de Montpellier, Equipe Labellisée Ligue contre le Cancer, Montpellier, 34293, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bodin
authors.affiliation: CRBM, CNRS, UMR 5237, Université de Montpellier, Equipe Labellisée Ligue contre le Cancer, Montpellier, 34293, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Delaval
authors.affiliation: CRBM, CNRS, UMR 5237, Université de Montpellier, Equipe Labellisée Ligue contre le Cancer, Montpellier, 34293, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Comunale
authors.affiliation: CRBM, CNRS, UMR 5237, Université de Montpellier, Equipe Labellisée Ligue contre le Cancer, Montpellier, 34293, France.|MRI-CRBM Imaging facility, CNRS, UMR 5237, Université de Montpellier, Montpellier, 34293, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Georget
authors.affiliation: Laboratório de Diferenciação Muscular e Citoesqueleto, Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21949-590, Brazil.
authors.forename: Manoel L
authors.initials: ML
authors.lastname: Costa
authors.affiliation: Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Université de Montpellier II et I, CNRS, UMR 5235, Montpellier, 34095, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Lutfalla
authors.affiliation: CRBM, CNRS, UMR 5237, Université de Montpellier, Equipe Labellisée Ligue contre le Cancer, Montpellier, 34293, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Gauthier-Rouvière
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D002450:Cell Communication; D002465:Cell Movement; D055785:Gene Knockdown Techniques; D008565:Membrane Proteins / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D008565:Membrane Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin; C106354:flotillins
keywords: E-cadherin; Flotillins; Gastrulation; Zebrafish