title: HDAC8 functions in spindle assembly during mouse oocyte meiosis.
abstract: HDAC8 is a class I histone deacetylase that functions in a variety of biological processes through its non-histone substrates. However, its roles during oocyte meiosis remain elusive. Here, we document that HDAC8 localizes at spindle poles and positively participates in the regulation of microtubule organization and spindle assembly in mouse oocytes. Depletion of HDAC8 by siRNA-based gene silencing results in various spindle defects and chromosome misalignment during oocyte meiotic maturation, accompanied by impaired kinetochore-microtubule attachments. Consequently, a higher incidence of aneuploidy is generated in HDAC8-depleted MII eggs. In addition, inhibition of HDAC8 activity with its selective inhibitor PCI-34051 phenocopies the spindle/chromosome defects resulting from HDAC8 depletion by siRNA injection. Finally, we find that HDAC8 is required for the correct localization of Ï-tubulin to spindle poles. Collectively, these data reveal that HDAC8 plays a significant role in regulating spindle assembly and thus ensuring the euploidy in mouse eggs.
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Kemei
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chaohua
authors.initials: C
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shu
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Yingjiao
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Ensheng
authors.initials: E
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Qinbo
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Reproductive Medicine Center, Ningbo First Hospital, Zhejiang 315010, China.
authors.forename: Yibo
authors.initials: Y
authors.lastname: Dai
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002478:Cells, Cultured; D020090:Chromosome Segregation / Q000187:drug effects; D005260:Female; D006655:Histone Deacetylases / Q000737:chemistry / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008941:Spindle Apparatus / Q000187:drug effects / Q000502:physiology*; D014404:Tubulin / Q000378:metabolism
chemical_list: D006877:Hydroxamic Acids; D007211:Indoles; C529435:PCI 34051; D014404:Tubulin; C000603105:HDAC8 protein, mouse; D006655:Histone Deacetylases
keywords: HDAC8; aneuploid eggs; chromosome alignment; kinetochore-microtubule attachment; spindle assembly