title: More significance of TB-IGRA except for the diagnose of tuberculosis.
abstract: OBJECTIVE
  Tuberculosis (TB)-interferon gamma release assay (IGRA) test has the characteristics of short time, high specificity, and high sensitivity, but it lacks the correlation research between TB-IGRA test results and body's immune cells, disease progression and prognosis, which is explored in this study.
  
  
  METHODS
  A retrospective study was carried out on positive TB-IGRA patients who were infected with TB and diagnosed at our hospital from January 2014 to June 2015. The TB-IGRA, routine blood test, T-cell subgroup data were collected for statistical analysis.
  
  
  RESULTS
  TB-IGRA results were in positive proportion to the lymphocytes, CD4+T cells and CD4+CD28+T cells, whereas negative to the Treg cells. Patient with unilateral pulmonary lesion had higher TB-IGRA than those with bilateral pulmonary lesions. After the stimulation of TB-specific antigen, the proportion of CD4+IFN-γ+and CD8+IFN-γ+T Tcells were both increased and the CD4+IFN-γ+T had positive correlation with the value of TB-IGRA.
  
  
  CONCLUSIONS
  IFN-γ was tested with TB-IGRA in patients with TB by the specific TB T cells and correlated with the lymphocytes, while the lymphocytes also closely related to the host's anti-TB immunity and disease outcome. Hence the result of TB-IGRA could reflect the specific anti-TB immunity ability of the host, disease progression and prognosis. This study further expands the application scope of TB-IGRA technology in the diagnosis of TB and lays a foundation for clinical practice to understand the immunity state of the patients with TB and the application of auxiliary clinical immunity regulators.
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Jun-Chi
authors.identifier: http://orcid.org/0000-0002-1447-7593
authors.initials: JC
authors.lastname: Xu
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.
authors.forename: Ze-Yi
authors.initials: ZY
authors.lastname: Li
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Xin-Nian
authors.initials: XN
authors.lastname: Chen
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.
authors.forename: Cui-Lin
authors.initials: CL
authors.lastname: Shi
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Wu
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhu
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Hua-Feng
authors.initials: HF
authors.lastname: Song
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Min-Juan
authors.initials: MJ
authors.lastname: Wu
authors.affiliation: The Fifth People's Hospital of Suzhou, Suzhou, China.|Key Laboratory of TB Prevention and Cure of Suzhou, Suzhou, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D059425:Interferon-gamma Release Tests / Q000706:statistics & numerical data*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D014376:Tuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology*
chemical_list: D007371:Interferon-gamma
keywords: 
  Mycobacterium tuberculosis
  ; TB-IGRA; γ-interferon