title: Lipidomics in research on yeast membrane lipid homeostasis.
abstract: Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors addressing the dynamics of lipid metabolism. Here my experience with, and view on mass spectrometry-based lipid analysis is presented, with emphasis on aspects of quantification of membrane lipid composition of the yeast Saccharomyces cerevisiae. This article is part of a Special Issue entitled: BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein.
authors.affiliation: Membrane Biochemistry & Biophysics, Bijvoet Center for Biomolecular Research and Institute of Biomembranes, Utrecht University, The Netherlands. Electronic address: a.i.p.m.dekroon@uu.nl.
authors.forename: Anton I P M
authors.initials: AIPM
authors.lastname: de Kroon
mesh_terms: D006706:Homeostasis / Q000502:physiology*; D007553:Isotope Labeling / Q000379:methods; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008563:Membrane Lipids / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods; D015003:Yeasts / Q000378:metabolism*
chemical_list: D008055:Lipids; D008563:Membrane Lipids
keywords: Lipidomics; Membrane lipid composition; Stable isotope labeling; Yeast lipids