title: Diversity synthesis of tetrahydroprotoberberines glycosides by combined chemical and microbial catalysis.
abstract: The present study was designed to construct the structurally diverse library of tetrahydroprotoberberines (THPBs) by combining the methods of chemical nonselective demethylation and microbial glycosylation. HPLC-MS/MS analyses tentatively identified 12 de-methylated and 9 glycosylated derivates of THPBs and 5 rarely oxidized glycosides of THPBs in the library. Through this effort, we achieved not only a variety of the THPBs and their glycosides but also tested the catalytic characteristics and capabilities of G. deliquescens NRRL 1086.
authors.affiliation: Department of Pharmacy, Huzhou University, Huzhou 313000, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Huzhou University, Huzhou 313000, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China. Electronic address: boyangyu59@163.com.
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Yu
authors.affiliation: Huzhou Food and Drug Inspection Institute, Huzhou 313000, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Chen
mesh_terms: D001600:Berberine Alkaloids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D002384:Catalysis; D020462:Gliocladium / Q000378:metabolism*; D006027:Glycosides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D015394:Molecular Structure
chemical_list: D001600:Berberine Alkaloids; D006027:Glycosides; C009090:protoberberine
keywords: Chemical demethylation; Diversity synthesis; Microbial glycosylation; THPBs library