title: Seed and peel essential oils obtained from Campomanesia adamantium fruit inhibit inflammatory and pain parameters in rodents.
abstract: Campomanesia adamantium (Myrtaceae) is popularly known as "gabiroba" and has been used in folk medicine as antirheumatic, antidiarrheal, hypocholesterolemic and anti-inflammatory. This study evaluated the anti-inflammatory and antinociceptive activities and toxicology of essential oils from peel (EOP) and seed (EOS) of C. adamantium fruits in animal models. Different groups were treated with doses of 100 and 300 mg/kg and the inflammatory parameters were evaluated in carrageenan induced paw oedema and leukocyte migration in pleurisy model, while antinociceptive activity was evaluated using formalin method in rodents. The major constituent of EOP and EOS was limonene with 13.07% and 20.89%, respectively. No clinical signs of toxicity have been observed in animals. It was observed a significant decreased (P<0.01) in leukocyte migration at the dose of 300 mg/kg of EOP and EOS, with maximal inhibition of 89±3% for EOP and 80±6% for EOS. Paw oedema was inhibited at all times, and maximal inhibition was at the dose of 100 mg/kg at 2 h after carrageenan injection with 72±2% for EOP and 74±2% for EOS. EOS and EOP also reduced the first and second phases of formalin-induced nociception test. In the first formalin-phase, maximal inhibitions were at 48±5% for EOP and 66±4% for EOS (300 mg/kg). At the inflammatory phase induced by formalin, maximal inhibitions were 72±2% for EOP and 80±2% for EOS at the dose of 100 mg/kg. Seed and peel essential oils from C. adamantium fruit inhibited leukocyte migration, inflammatory and neurogenic pain and oedema suggesting their use as nutraceutical or pharmacological agent.
authors.affiliation: College of Exact and Technological Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
authors.forename: Danieli
authors.initials: D
authors.lastname: Zuntini Viscardi
authors.affiliation: College of Exact and Technological Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
authors.forename: Jucicléia da Silva
authors.initials: JD
authors.lastname: Arrigo
authors.affiliation: College of Exact and Technological Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
authors.forename: Camila de Azevedo Chaves
authors.initials: CA
authors.lastname: Correia
authors.affiliation: College of Health Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
authors.forename: Cândida Aparecida Leite
authors.initials: CA
authors.lastname: Kassuya
authors.affiliation: Course of Chemistry, State University of Mato Grosso do Sul, Dourados, Mato Grosso do Sul, Brazil.
authors.forename: Claudia Andrea Lima
authors.initials: CA
authors.lastname: Cardoso
authors.affiliation: Brazilian Agricultural Research Agency (Embrapa), Brasília, Federal District, Brazil.
authors.forename: Iriani Rodrigues
authors.initials: IR
authors.lastname: Maldonade
authors.affiliation: College of Food Engineering, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
authors.forename: Eliana Janet Sanjinez
authors.initials: EJ
authors.lastname: Argandoña
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002351:Carrageenan / Q000633:toxicity; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003080:Cold Temperature / Q000009:adverse effects; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000188:drug therapy; D005260:Female; D005557:Formaldehyde / Q000633:toxicity; D005638:Fruit / Q000737:chemistry; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy / Q000209:etiology; D027841:Pimenta / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D010998:Pleurisy / Q000188:drug therapy; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D005557:Formaldehyde; D002351:Carrageenan