title: The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II.
abstract: Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants in legume are stay-green mutants, in which chlorophyll degradation is impaired during leaf senescence and seed maturation. Among them, the soybean (Glycine max) green cotyledon genecytGis unique because it is maternally inherited. To isolatecytG, we extensively sequenced the soybean chloroplast genome, and detected a 5-bp insertion causing a frame-shift inpsbM, which encodes one of the small subunits of photosystem II. Mutant tobacco plants (Nicotiana tabacum) with a disruptedpsbMgenerated using a chloroplast transformation technique had green senescent leaves, confirming thatcytGencodes PsbM. The phenotype ofcytGwas very similar to that of mutant of chlorophyllbreductase catalyzing the first step of chlorophyllbdegradation. In fact, chlorophyllb-degrading activity in dark-growncytGandpsbM-knockout seedlings was significantly lower than that of wild-type plants. Our results suggest that PsbM is a unique protein linking photosynthesis in presenescent leaves with chlorophyll degradation during leaf senescence and seed maturation. Additionally, we discuss the origin ofcytG, which may have been selected during domestication of soybean.
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Kaori
authors.initials: K
authors.lastname: Kohzuma
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ito
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Ayako
authors.initials: A
authors.lastname: Okuzaki
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Mai
authors.identifier: 0000-0002-1785-3494
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Hideki
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Michiharu
authors.initials: M
authors.lastname: Nakano
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamatani
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Yu
authors.identifier: 0000-0002-8772-3767
authors.initials: Y
authors.lastname: Masuda
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Yumi
authors.initials: Y
authors.lastname: Nagashima
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukuoka
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Akira
authors.identifier: 0000-0003-3899-543X
authors.initials: A
authors.lastname: Kanazawa
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kitamura
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tabei
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Wataru
authors.initials: W
authors.lastname: Sakamoto
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.).|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.).|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.).|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.).|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.).|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.).
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.); akusaba@hiroshima-u.ac.jp.|Institute of Crop Science, NARO, Tsukuba, Ibaraki 305-8518, Japan (Y.S.); akusaba@hiroshima-u.ac.jp.|Institute of Agrobiological Sciences, NARO Tsukuba, Ibaraki 305-8602, Japan (A.O., Y.T.); akusaba@hiroshima-u.ac.jp.|Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan (H.I., A.T.); akusaba@hiroshima-u.ac.jp.|Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan (M.W., M.I.); akusaba@hiroshima-u.ac.jp.|NARO Institute of Vegetable and Tea Science, Tsu, Mie 514-2392, Japan (H.F.); akusaba@hiroshima-u.ac.jp.|Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan (H.K., T.Y., A.K., K.K.); and akusaba@hiroshima-u.ac.jp.|Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan (W.S.) akusaba@hiroshima-u.ac.jp.
authors.forename: Makoto
authors.identifier: 0000-0003-1200-7640
authors.initials: M
authors.lastname: Kusaba
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D055162:Biocatalysis; D015153:Blotting, Western; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D018548:Cotyledon / Q000235:genetics* / Q000378:metabolism; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D010641:Phenotype; D045332:Photosystem II Protein Complex / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits; D002734:Chlorophyll; C037184:chlorophyll b; D000429:Alcohol Oxidoreductases; C102575:chlorophyll b reductase