title: Enrichment and Viability Inhibition of Circulating Tumor Cells on a Dual Acid-Responsive Composite Nanofiber Film.
abstract: The formation and metastatic colonization of circulating tumor cells (CTCs) are responsible for the vast majority of cancer-related deaths. Over the last decade, drug-delivery systems (DDSs) have rapidly developed with the emergence of nanotechnology; however, most reported tumor-targeting DDSs are able to deliver drugs only to solid tumor cells and not CTCs. Herein, a novel DDS comprising a composite nanofiber film was constructed to inhibit the viability of CTCs. In this system, gold nanoparticles (Au NPs) were functionalized with doxorubicin (DOX) through an acid-responsive cleavable linker to obtain Au-DOX NPs. Then, the Au-DOX NPs were mixed in a solution of an acid-responsive polymer {i.e., poly[2-(dimethylamino)ethyl methacrylate]} to synthesize the nanofiber film through electrospinning technology. After that, the nanofiber film was modified with a specific antibody (i.e., anti-EpCAM) to enrich the concentration of CTCs on the film. Finally, the Au-DOX NPs were released from the nanofiber film, and they consequently inhibited the viability of CTCs by delivering DOX to the enriched CTCs. This composite nanofiber film was able to decrease the viability of CTCs significantly in the suspended and fluid states, and it is expected to limit the migration and proliferation of tumor cells.
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Wang
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Yaya
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Xu
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Research Center for Bioengineering & Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000633:toxicity*; D004337:Drug Carriers / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D006046:Gold / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D009360:Neoplastic Cells, Circulating / Q000187:drug effects*; D009757:Nylons / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D008689:Methacrylates; D009757:Nylons; C407037:poly(2-(dimethylamino)ethyl methacrylate); D006046:Gold; D004317:Doxorubicin
keywords: antitumor agents; cancer; circulating tumor cells; drug delivery; nanofibers