title: DNA methylation pattern of apoptosis-related genes in ameloblastoma.
abstract: OBJECTIVE
  DNA methylation is an important mechanism of gene control expression, and it has been poorly addressed in odontogenic tumours. On this basis, we aimed to assess the methylation pattern of 22 apoptosis-related genes in solid ameloblastomas.
  
  
  METHODS
  Ameloblastoma fresh samples (n = 10) and dental follicles (n = 8) were included in the study. The percentage fraction of methylated and unmethylated DNA promoter of 22 apoptosis-related genes was determined using enzymatic restriction digestion and quantitative real-time PCR (qPCR) array. The relative expressions of the genes that showed the most discrepant methylation profile between tumours and controls were analysed by reverse-transcription quantitative PCR (RT-qPCR).
  
  
  RESULTS
  Lower methylation percentages of TNFRSF25 (47.2%) and BCL2L11 (33.2%) were observed in ameloblastomas compared with dental follicles (79.3% and 59.5%, respectively). The RT-qPCR analysis showed increased expression of BCL2L11 in ameloblastomas compared with dental follicles, in agreement with the methylation analysis results, while there was no difference between the expression levels of TNFRSF25 between both groups.
  
  
  CONCLUSIONS
  On the basis of our results, the transcription of the apoptosis-related gene BCL2L11 is possibly regulated by promoter DNA methylation in ameloblastoma. The biological significance of this finding in ameloblastoma pathobiology remains to be clarified.
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Sfs
authors.initials: S
authors.lastname: Costa
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: N B
authors.initials: NB
authors.lastname: Pereira
authors.affiliation: School of Dentistry, Universidade Federal do Ceará, Sobral, Brazil.
authors.forename: Kma
authors.identifier: http://orcid.org/0000-0002-2880-6466
authors.initials: K
authors.lastname: Pereira
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: K
authors.initials: K
authors.lastname: Campos
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: W H
authors.initials: WH
authors.lastname: de Castro
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: Diniz
authors.affiliation: Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Gomes
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: R S
authors.identifier: http://orcid.org/0000-0001-8770-8009
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000328:Adult; D000564:Ameloblastoma / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics; D000072224:Bcl-2-Like Protein 11 / Q000235:genetics*; D016022:Case-Control Studies; D019175:DNA Methylation; D005260:Female; D015870:Gene Expression; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D053219:Receptors, Tumor Necrosis Factor, Member 25 / Q000235:genetics*; D055815:Young Adult
chemical_list: C000606775:BCL2L11 protein, human; D000072224:Bcl-2-Like Protein 11; D053219:Receptors, Tumor Necrosis Factor, Member 25; C506033:TNFRSF25 protein, human
keywords: Bcl-2-like protein 11; DNA methylation; TNFRSF25; ameloblastoma; apoptosis