title: Redox regulation of SurR by protein disulfide oxidoreductase in Thermococcus onnurineus NA1.
abstract: Protein disulfide oxidoreductases are redox enzymes that catalyze thiol-disulfide exchange reactions. These enzymes include thioredoxins, glutaredoxins, protein disulfide isomerases, disulfide bond formation A (DsbA) proteins, and Pyrococcus furiosus protein disulfide oxidoreductase (PfPDO) homologues. In the genome of a hyperthermophilic archaeon, Thermococcus onnurineus NA1, the genes encoding one PfPDO homologue (TON_0319, Pdo) and three more thioredoxin- or glutaredoxin-like proteins (TON_0470, TON_0472, TON_0834) were identified. All except TON_0470 were recombinantly expressed and purified. Three purified proteins were reduced by a thioredoxin reductase (TrxR), indicating that each protein can form redox complex with TrxR. SurR, a transcription factor involved in the sulfur response, was tested for a protein target of a TrxR-redoxin system and only Pdo was identified to be capable of catalyzing the reduction of SurR. Electromobility shift assay demonstrated that SurR reduced by the TrxR-Pdo system could bind to the DNA probe with the SurR-binding motif, GTTttgAAC. In this study, we present the TrxR-Pdo couple as a redox-regulator for SurR in T. onnurineus NA1.
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 15627, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea.
authors.forename: Jae Kyu
authors.initials: JK
authors.lastname: Lim
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 15627, Republic of Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea.
authors.forename: Hae-Chang
authors.initials: HC
authors.lastname: Jung
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 15627, Republic of Korea. sgkang@kiost.ac.kr.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea. sgkang@kiost.ac.kr.
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 15627, Republic of Korea. leeh522@kiost.ac.kr.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea. leeh522@kiost.ac.kr.
authors.forename: Hyun Sook
authors.identifier: http://orcid.org/0000-0002-5092-5285
authors.initials: HS
authors.lastname: Lee
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding; D017385:Sequence Homology; D013455:Sulfur / Q000378:metabolism; D019712:Thermococcus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D013455:Sulfur; D013880:Thioredoxin-Disulfide Reductase
keywords: Protein disulfide oxidoreductase; Redox system; SurR; Thermococcus onnurineus NA1; Thioredoxin reductase