title: Anti-inflammatory Role of Pilose Antler Peptide in LPS-Induced Lung Injury.
abstract: The present study was designed to investigate the effects of pilose antler peptide (PAP) on lipopolysaccharide (LPS)-induced lung injury. BalB/c mice intraperitoneally received PAP (10 and 20 mg/kg) or dexamethasone (2 mg/kg) 1 h prior to intratracheal instillation of LPS. PAP significantly decreased lung wet-to-dry weight (W/D) ratio and lung myeloperoxidase (MPO) activity and restored LPS-induced lung histopathological changes. PAP also increased super oxide dismutase (SOD) level and inhibited malondialdehyde (MDA) content and levels of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) in LPS-stimulated mice. Furthermore, we demonstrated that PAP inhibited Rho/NF-κB pathway in LPS-induced mice. Our experimental results indicated that the protective mechanism of PAP might be attributed partly to the inhibition of Rho/NF-κB pathway.
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing, 210001, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Ma
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing, 210001, China. longhongyantcm@126.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Long
authors.affiliation: The First Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, 130117, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Yang
authors.affiliation: The First Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, 130117, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Cai
authors.affiliation: Changchun University of Chinese Medicine, Changchun, 130117, China. zhangtz452@126.com.
authors.forename: Tianzhu
authors.initials: T
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, 130117, China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides; D055370:Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D010088:Oxidoreductases / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology* / Q000627:therapeutic use; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010455:Peptides; C000626469:pilose antler peptide; D010088:Oxidoreductases; D020741:rho GTP-Binding Proteins
keywords: LPS; Rho/NF-κB; lung injury; pilose antler peptide