title: Effect of selenium supplementation on semen characteristics of Brazil's ram.
abstract: The aim of this study was to investigate the effects of different concentrations of oral supplementation with selenium (Se) upon ram sperm parameters. Thirty rams managed in stall under intensive system were used and divided into five groups (six animals per group) as follows: control group (G1) mineral mixture supplementation without Se, group 2 (G2) mineral mixture supplemented with 5 mg/kg Se, group 3 (G3) supplemented with 10 mg/kg Se, group 4 (G4) supplemented with 15 mg/kg Se and group 5 (G5) supplemented with 20 mg/kg Se. For each group, there was an adjustment period of 14 days. The experimental period was 350 days. Every 56 days, the animals were weighed and semen samples were collected by electroejaculation. Semen analysis included volume, mass moviment, total motility, vigour, concentration and morphology. For plasmatic and acrosomal membrane integrity evaluation and mitochondrial membrane potential were used a combination of fluorescent probes. Differences between means values obtained by analysis of variance were verified by Tukey test with 5% probability. There was no statistical difference between treatment groups in relation to volume, mass moviment, total motility, vigour, concentration, plasma and acrosomal membrane integrity (p > .05). Sperm morphology was different between treatment groups, the G1 (0 mg of selenium) had the highest percentage of major defects (11.11 ± 1.11a; p < .05). It was concluded that selenium decrease the percentage of sperm defects and did not directly influence on ram sperm volume, mass moviment, total motility, vigour, concentration and membrane integrity.
authors.affiliation: School of Veterinary Medicine and Animal Science, Sao Paulo State University - UNESP, Botucatu, SP, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Piagentini
authors.affiliation: School of Veterinary Medicine and Animal Science, Sao Paulo State University - UNESP, Botucatu, SP, Brazil.
authors.forename: D C
authors.initials: DC
authors.lastname: Silva
authors.affiliation: School of Veterinary Medicine and Animal Science, Sao Paulo State University - UNESP, Botucatu, SP, Brazil.
authors.forename: Cpf
authors.initials: C
authors.lastname: Dell'Aqua
authors.affiliation: School of Veterinary Medicine, State University Midwest - UNICENTRO, Guarapuava, PR, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: Moya-Araujo
authors.affiliation: School of Veterinary Medicine and Animal Science, Sao Paulo State University - UNESP, Botucatu, SP, Brazil.
authors.forename: V M
authors.initials: VM
authors.lastname: Codognoto
authors.affiliation: School of Veterinary Medicine and Animal Science, Sao Paulo State University - UNESP, Botucatu, SP, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Ramos
authors.affiliation: School of Veterinary Medicine and Animal Science, Sao Paulo State University - UNESP, Botucatu, SP, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Oba
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D001938:Brazil; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D012643:Selenium / Q000494:pharmacology*; D012661:Semen / Q000187:drug effects* / Q000502:physiology; D055101:Semen Analysis / Q000662:veterinary; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D012643:Selenium
keywords: ram; semen; trace minerals