title: [Effect of Homoharringtonine Combined with Imatinib on the K562/G01 Cells and Its Mechanism].
abstract: OBJECTIVE
  To explore the effect of homoharringtonine(HHT) combined with imatinib(IM) on proliferation and apoptosis of K562/G01 cells and its potential mechanism.
  
  
  METHODS
  K562/G01 cells were cultured with HHT and/or IM. CCK-8 assay was used to detect cell proliferation. Cell apoptosis and phosphorylated tyrosine levels were analyzed by flow cytometry. The expression levels of p210, PI3K, p-Akt and Akt protein were determined by Western blot.
  
  
  RESULTS
  Compared with HHT or IM alone, drug combination significantly inhibited cell proliferation and induced apoptosis of K562/G01 cells (both P< 0.05). HHT combined with IM could inhibit the levels of phosphorylated tyrosine and phosphorylated Crkl and downregulate the expressions of p210, PI3K and p-Akt in K562/G01 cells.
  
  
  CONCLUSIONS
  HHT combined with IM can synergistically inhibit proliferation and induce apoptosis of K562/G01 cells by suppressing the p210 expression and its kinase activity.
authors.affiliation: Department of Hematology, Nanjing Medical University Affiliated Huai'an First Hospital, Huai'an 223300, Jiangsu Province, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Wu
authors.affiliation: Department of Hematology, Nanjing Medical University Affiliated Huai'an First Hospital, Huai'an 223300, Jiangsu Province, China.
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Ding
authors.affiliation: Department of Hematology, Nanjing Medical University Affiliated Huai'an First Hospital, Huai'an 223300, Jiangsu Province, China.
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Deng
authors.affiliation: Department of Hematology, Nanjing Medical University Affiliated Huai'an First Hospital, Huai'an 223300, Jiangsu Province, China.
authors.forename: Yu-Ye
authors.initials: YY
authors.lastname: Shi
authors.affiliation: Department of Hematology, Nanjing Medical University Affiliated Huai'an First Hospital, Huai'an 223300, Jiangsu Province, China.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Lu
authors.affiliation: Department of Hematology, Nanjing Medical University Affiliated Huai'an First Hospital, Huai'an 223300, Jiangsu Province, China. E-mail: liyufeng99@netease.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D000068877:Imatinib Mesylate / Q000494:pharmacology*; D020014:K562 Cells; D019869:Phosphatidylinositol 3-Kinases
chemical_list: D000970:Antineoplastic Agents; D006248:Harringtonines; D000077863:Homoharringtonine; D000068877:Imatinib Mesylate; D019869:Phosphatidylinositol 3-Kinases