title: Effect of hypoxia on human adipose-derived mesenchymal stem cells and its potential clinical applications.
abstract: Human adipose-derived mesenchymal stem cells (hASCs) are an ideal cell source for regenerative medicine due to their capabilities of multipotency and the readily accessibility of adipose tissue. They have been found residing in a relatively low oxygen tension microenvironment in the body, but the physiological condition has been overlooked in most studies. In light of the escalating need for culturing hASCs under their physiological condition, this review summarizes the most recent advances in the hypoxia effect on hASCs. We first highlight the advantages of using hASCs in regenerative medicine and discuss the influence of hypoxia on the phenotype and functionality of hASCs in terms of viability, stemness, proliferation, differentiation, soluble factor secretion, and biosafety. We provide a glimpse of the possible cellular mechanism that involved under hypoxia and discuss the potential clinical applications. We then highlight the existing challenges and discuss the future perspective on the use of hypoxic-treated hASCs.
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603, Kuala Lumpur, Malaysia. janeruchoi@gmail.com.
authors.forename: Jane Ru
authors.initials: JR
authors.lastname: Choi
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603, Kuala Lumpur, Malaysia.
authors.forename: Kar Wey
authors.initials: KW
authors.lastname: Yong
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603, Kuala Lumpur, Malaysia. wansafwani@um.edu.my.
authors.forename: Wan Kamarul Zaman
authors.initials: WKZ
authors.lastname: Wan Safwani
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D015687:Cell Hypoxia; D002986:Clinical Trials as Topic; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008954:Models, Biological
keywords: Challenges; Clinical applications; Functionality; Hypoxia; Phenotype; hASCs