title: Hymenobacter knuensis sp. nov., Isolated From River Water.
abstract: A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterial strain, designated 16F7C-2T, was isolated from the Han River, South Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 16F7C-2Tbelonged to the family Cytophagaceae in the phylum Bacteroidetes and was most closely related to Hymenobacter algoricola VUG-A23aT(98.3%) and Hymenobacter fastidiosus VUG-A124T(97.7%). The G + C content of the genomic DNA of strain 16F7C-2Twas 63.4 mol%. The detection of menaquinone MK-7 as the predominant respiratory quinone; a fatty acid profile with summed feature 3 (C16:1ω7c/C16:1ω6c; 19.5%), summed feature 4 (C17:1iso I/C17:1anteiso B; 15.9%), C15:0iso (12.6%), C16:1ω5c (10.5%), and C15:0anteiso (10.4%) as the major components; and a polar lipid profile with phosphatidylethanolamine as the major component also supported the affiliation of strain 16F7C-2Tto the genus Hymenobacter. The DNA-DNA relatedness between strain 16F7C-2Tand H. algoricola JCM 17214Tand H. fastidiosus JCM17224Twere 45.2 ± 5.8 and 40.3 ± 2.9%, respectively, clearly showing that the isolate constitutes a new genospecies. Strain 16F7C-2Tcould be clearly differentiated from its closest neighbors on the basis of its phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 16F7C-2Trepresents a novel species of the genus Hymenobacter, for which the name Hymenobacter knuensis sp. nov. is proposed. The type strain is 16F7C-2T(=KCTC 52538T= JCM 31814T).
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Horticultural Science, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Kang
authors.affiliation: Department of Bio and Environmental Technology, Seoul Women's University, Seoul, 01797, Republic of Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.|Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
mesh_terms: D041963:Bacteroidetes / Q000145:classification / Q000235:genetics* / Q000502:physiology; D001482:Base Composition / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine