title: NFATc1 phosphorylation by DYRK1A increases its protein stability.
abstract: NFATs are transcription factors involved in immune activation and tumor progression. Previous reports showed that DYRK1A suppressed NFATc2 transcriptional activity through phosphorylation. Nonetheless, our results showed that DYRK1A increased NFATc1/αA protein level and subsequent transcriptional activity. DYRK1A phosphorylation of NFATc1/αA at S261, S278, S403 and S409 interfered with NFATc1 ubiquitination and ubiquitin-proteasome degradation. Our results imply that DYRK1A is a positive kinase in regulation of NFATc1.
authors.affiliation: Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong Province, China.|Otolaryngology Key Lab of Ministry of Health, Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong Province, China.
authors.forename: Ketao
authors.initials: K
authors.lastname: Wang
authors.affiliation: Otolaryngology Key Lab of Ministry of Health, Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong Province, China.|Otolaryngology Key Lab of Ministry of Health, Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong Province, China.
authors.forename: Yuankai
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Otolaryngology Key Lab of Ministry of Health, Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong Province, China.|Brain Research Institute, Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
authors.forename: Xiulian
authors.initials: X
authors.lastname: Sun
mesh_terms: D020816:Amino Acid Motifs; D057809:HEK293 Cells; D006801:Humans; D050778:NFATC Transcription Factors / Q000378:metabolism*; D010766:Phosphorylation; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D055550:Protein Stability; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D059748:Proteolysis; D054875:Ubiquitination; D000097603:Dyrk Kinases
chemical_list: D050778:NFATC Transcription Factors; D046988:Proteasome Endopeptidase Complex; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D000097603:Dyrk Kinases; C494003:NFATC1 protein, human