title: Unique Lipid Chemistry of Synaptic Vesicle and Synaptosome Membrane Revealed Using Mass Spectrometry.
abstract: Synaptic vesicles measuring 30-50 nm in diameter containing neurotransmitters either completely collapse at the presynaptic membrane or dock and transiently fuse at the base of specialized 15 nm cup-shaped lipoprotein structures called porosomes at the presynaptic membrane of synaptosomes to release neurotransmitters. Recent study reports the unique composition of major lipids associated with neuronal porosomes. Given that lipids greatly influence the association and functions of membrane proteins, differences in lipid composition of synaptic vesicle and the synaptosome membrane was hypothesized. To test this hypothesis, the lipidome of isolated synaptosome, synaptosome membrane, and synaptic vesicle preparation were determined by using mass spectrometry in the current study. Results from the study demonstrate the enriched presence of triacyl glycerols and sphingomyelins in synaptic vesicles, as opposed to the enriched presence of phospholipids in the synaptosome membrane fraction, reflecting on the tight regulation of nerve cells in compartmentalization of membrane lipids at the nerve terminal.
authors.affiliation: Department of Physiology and ‡Department of Pathology, Lipidomics Core Facility, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Kenneth T
authors.initials: KT
authors.lastname: Lewis
authors.affiliation: Department of Physiology and ‡Department of Pathology, Lipidomics Core Facility, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Krishna R
authors.initials: KR
authors.lastname: Maddipati
authors.affiliation: Department of Physiology and ‡Department of Pathology, Lipidomics Core Facility, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Akshata R
authors.initials: AR
authors.lastname: Naik
authors.affiliation: Department of Physiology and ‡Department of Pathology, Lipidomics Core Facility, Wayne State University School of Medicine , Detroit, Michigan 48201, United States.
authors.forename: Bhanu P
authors.identifier: 0000-0002-6030-8766
authors.initials: BP
authors.lastname: Jena
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002462:Cell Membrane / Q000737:chemistry; D013058:Mass Spectrometry; D008563:Membrane Lipids / Q000737:chemistry*; D051381:Rats; D013572:Synaptic Vesicles / Q000737:chemistry*; D013574:Synaptosomes / Q000737:chemistry*
chemical_list: D008563:Membrane Lipids
keywords: Synaptosome; mass spectrometry; synaptic vesicle lipids