title: Association of single nucleotide polymorphisms of IL23R and IL17 with necrotizing enterocolitis in premature infants.
abstract: Necrotizing enterocolitis (NEC) is a severe gastrointestinal inflammatory disease in neonates, particularly in preterm infants. The interleukin (IL) 23/IL17 axis has been shown to play an important role in the gastrointestinal inflammation. However, the association of gene polymorphisms in the IL23/IL17 axis and the development of NEC remains unknown. In this study, we aimed to explore a possible genetic role of IL23R and IL17 in the development of NEC. We identified single nucleotide polymorphisms (SNPs) in IL23R (rs10889677), IL17A (rs2275913), and IL17F (rs763780) by polymerase chain reaction and Sanger sequencing. A total of 102 NEC patients (stage II, n = 75; and stage III, n = 27) and 120 control subjects were recruited for the study. All of the participants were premature (gestational age < 37 weeks). Our results revealed that the combination of the IL17F rs763780 (TC + CC) genotype and the C allele both significantly increased the risk of NEC [odds ratio (OR) 1.89, 95% confidence interval (CI) 1.04-3.43, P = 0.035; OR 1.82, 95% CI 1.06-3.13, P = 0.028, respectively]. Furthermore, the rs763780 (TC + CC) genotype was associated with increased severity of NEC and the incidence of NEC-related perforation [OR 2.80, 95% CI 1.10-7.12, P = 0.031; OR 3.86, 95% CI 1.10-13.53, P = 0.035, respectively]. However, IL23R rs10889677 and IL17A rs2275913 were not associated with the susceptibility to NEC. In conclusion, our data suggest that a variant of IL17F (rs763780) may contribute to the development of NEC.
authors.affiliation: Department of Neonatology, The First Hospital of Jilin University, Changchun, China.|Department of Central Laboratory, The First Hospital of Jilin University, Changchun, China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Neonatology, The First Hospital of Jilin University, Changchun, China.|Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Sciences, University of California Los Angeles (UCLA) School of Medicine, Los Angeles, CA, USA.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Central Laboratory, The First Hospital of Jilin University, Changchun, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Central Laboratory, The First Hospital of Jilin University, Changchun, China.|Department of Orthopedics Surgery, The Second Hospital of Jilin University, Changchun, China.
authors.forename: Haohan
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Neonatology, The First Hospital of Jilin University, Changchun, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Neonatology, The Second Hospital of Jilin University, Changchun, China.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Central Laboratory, The First Hospital of Jilin University, Changchun, China. lipingpeng0116@sina.com.|Department of Respirology, The First Hospital of Jilin University, Changchun, China. lipingpeng0116@sina.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Neonatology, The First Hospital of Jilin University, Changchun, China. yanchaoying224@sina.com.
authors.forename: Chaoying
authors.initials: C
authors.lastname: Yan
mesh_terms: D020345:Enterocolitis, Necrotizing / Q000235:genetics*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000235:genetics*; D020381:Interleukin-17 / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D018123:Receptors, Interleukin / Q000235:genetics*
chemical_list: C508717:IL17A protein, human; C440021:IL17F protein, human; C515500:IL23R protein, human; D020381:Interleukin-17; D018123:Receptors, Interleukin
keywords: IL17/IL23R; Necrotizing enterocolitis; Neonates; Preterm; Single nucleotide polymorphisms