title: Development of a real-time PCR assay for the direct detection of Candida species causing Vulvovaginal candidiasis.
abstract: Identification of Candida species by traditional methods can be time-consuming and have limited analytical sensitivity. We developed a multiplex real-time PCR assay for detection and differentiation of Candida species causing vulvovaginal candidiasis (VVC). Overall, this PCR assay is a powerful diagnostic tool offering superior accuracy, sensitivity, and specificity.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT. Electronic address: keith.d.tardif@aruplab.com.
authors.forename: Keith D
authors.initials: KD
authors.lastname: Tardif
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT; University of Utah School of Medicine, Department of Pathology, Salt Lake City, UT.
authors.forename: Robert
authors.initials: R
authors.lastname: Schlaberg
mesh_terms: D002175:Candida / Q000235:genetics / Q000302:isolation & purification*; D002181:Candidiasis, Vulvovaginal / Q000175:diagnosis* / Q000382:microbiology*; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
keywords: Assay development; Candidiasis; Diagnostic; PCR; Vulvovaginal