title: Backbone1H,13C, and15N NMR resonance assignments of the Krüppel-like factor 4 activation domain.
abstract: Krüppel-like factor 4 (KLF4) is a transcription factor involved in diverse biological processes, including development, cellular differentiation and proliferation, and maintenance of tissue homeostasis. KLF4 has also been associated with disease states, such as cardiovascular disease and several cancers. KLF4 contains an activation domain, repression domain, and a structurally characterized C-terminal zinc finger domain that mediates its binding to DNA. The structurally uncharacterized KLF4 activation domain is critical for transactivation by KLF4 and mediates its binding to the transcriptional coactivator CBP/p300. Here, we report the1H,15N,13CO,13Cα and13Cβ NMR chemical shift assignments of KLF41-130, which contains the KLF4 activation domain. Narrow chemical shift dispersion in the1H dimension of the1H-15N HSQC spectrum suggests that the KLF41-130fragment is intrinsically disordered.
authors.affiliation: Department of Biomedical & Molecular Sciences, Queen's University, Kingston, ON, Canada, K7L 3N6.
authors.forename: Brigid S
authors.initials: BS
authors.lastname: Conroy
authors.affiliation: Department of Biomedical & Molecular Sciences, Queen's University, Kingston, ON, Canada, K7L 3N6.
authors.forename: Emma R
authors.initials: ER
authors.lastname: Weiss
authors.affiliation: Department of Biomedical & Molecular Sciences, Queen's University, Kingston, ON, Canada, K7L 3N6. steven.smith@queensu.ca.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Smith
authors.affiliation: Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R2. david.langelaan@dal.ca.
authors.forename: David N
authors.identifier: 0000-0001-9592-3075
authors.initials: DN
authors.lastname: Langelaan
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors
keywords: Activation domain; Intrinsically disordered protein; Krüppel-like factor 4; NMR resonance assignment; Transcription factor