title: TRPA1 mediated aggravation of allergic contact dermatitis induced by DINP and regulated by NF-κB activation.
abstract: The possible pathogenic role and mechanism of Di-iso-nonyl phthalate (DINP) in allergic dermatitis is still controversial. This work has shown that oral exposure to DINP exacerbated allergic dermatitis tissue lesions in FITC-sensitized mice. The lesions was accompanied by an enhancement of TRPA1 expression and an increase in IgG1, IL-6 and IL-13 levels. This work also found that blocking TRPA1 by HC030031 effectively prevented the development of allergic dermatitis resulting from oral exposure to DINP and/or FITC-sensitized mice. This result is marked by the down regulation of IgG1 levels, a reduction in mast cell degranulation and a decrease in IL-6 and IL-13 levels. We also showed that blocking NF-κB inhibited TRPA1 expression, and that blocking TRPA1 had no significant effect on the activation of NF-κB or TSLP expression. This study helps in understanding the role DINP exposure plays in the development of allergic dermatitis and provides new insight into the mechanisms behind the DINP-induced adjuvant effect.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors.forename: Baizhan
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Chen
mesh_terms: D000083:Acetanilides / Q000494:pharmacology; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D010795:Phthalic Acids / Q000009:adverse effects*; D011687:Purines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D000074025:TRPA1 Cation Channel / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: C552888:2-(1,3-dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-7H-purin-7-yl)-N-(4-isopropylphenyl)acetamide; D000083:Acetanilides; D016328:NF-kappa B; D010795:Phthalic Acids; D011687:Purines; D000074025:TRPA1 Cation Channel; C012125:diisononyl phthalate