title: IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis.
abstract: Repair of DNA double-strand breaks (DSBs) is critical for the maintenance of genome integrity. We previously showed that DSB-induced small RNAs (diRNAs) facilitate homologous recombination-mediated DSB repair inArabidopsis thalianaHere, we show that INVOLVED IN DE NOVO2 (IDN2), a double-stranded RNA binding protein involved in small RNA-directed DNA methylation, is required for DSB repair in Arabidopsis. We find that IDN2 interacts with the heterotrimeric replication protein A (RPA) complex. Depletion of IDN2 or the diRNA binding ARGONAUTE2 leads to increased accumulation of RPA at DSB sites and mislocalization of the recombination factor RAD51. These findings support a model in which IDN2 interacts with RPA and facilitates the release of RPA from single-stranded DNA tails and subsequent recruitment of RAD51 at DSB sites to promote DSB repair.
authors.affiliation: Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing 100871, China.|National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Mingming
authors.identifier: http://orcid.org/0000-0002-9348-8994
authors.initials: M
authors.lastname: Liu
authors.affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Ba
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Pedro
authors.identifier: http://orcid.org/0000-0002-2033-6519
authors.initials: P
authors.lastname: Costa-Nunes
authors.affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Lanxia
authors.initials: L
authors.lastname: Li
authors.affiliation: Bionova (Beijing) Biotech Co., Beijing 102206, China.
authors.forename: Fansi
authors.initials: F
authors.lastname: Kong
authors.affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.|Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
authors.forename: Jijie
authors.initials: J
authors.lastname: Chai
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Olga
authors.initials: O
authors.lastname: Pontes
authors.affiliation: Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China qiyijun@tsinghua.edu.cn.|Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
authors.forename: Yijun
authors.identifier: http://orcid.org/0000-0002-7100-1700
authors.initials: Y
authors.lastname: Qi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics / Q000502:physiology; D059765:Homologous Recombination / Q000235:genetics / Q000502:physiology; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051135:Rad51 Recombinase / Q000235:genetics / Q000378:metabolism; D051716:Replication Protein A / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C545931:IDN2 protein, Arabidopsis; D016601:RNA-Binding Proteins; D051716:Replication Protein A; C495146:ATRAD51 protein, Arabidopsis; D051135:Rad51 Recombinase