title: Metabolic engineering of Clostridium tyrobutyricum for n-butanol production from sugarcane juice.
abstract: Clostridium tyrobutyricum is a promising organism for butyrate and n-butanol production, but cannot grow on sucrose. Three genes (scrA, scrB, and scrK) involved in the sucrose catabolic pathway, along with an aldehyde/alcohol dehydrogenase gene, were cloned from Clostridium acetobutylicum and introduced into C. tyrobutyricum (Δack) with acetate kinase knockout. In batch fermentation, the engineered strain Ct(Δack)-pscrBAK produced 14.8-18.8 g/L butanol, with a high butanol/total solvent ratio of ∼0.94 (w/w), from sucrose and sugarcane juice. Moreover, stable high butanol production with a high butanol yield of 0.25 g/g and productivity of 0.28 g/L∙h was obtained in batch fermentation without using antibiotics for selection pressure, suggesting that Ct(Δack)-pscrBAK is genetically stable. Furthermore, sucrose utilization by Ct(Δack)-pscrBAK was not inhibited by glucose, which would usually cause carbon catabolite repression on solventogenic clostridia. Ct(Δack)-pscrBAK is thus advantageous for use in biobutanol production from sugarcane juice and other sucrose-rich feedstocks.
authors.affiliation: Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.|William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH, 43210, USA.
authors.forename: Jianzhi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH, 43210, USA.
authors.forename: Le
authors.initials: L
authors.lastname: Yu
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH, 43210, USA.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Xu
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH, 43210, USA. yang.15@osu.edu.
authors.forename: Shang-Tian
authors.identifier: http://orcid.org/0000-0002-5990-8529
authors.initials: ST
authors.lastname: Yang
authors.affiliation: Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
authors.forename: Qiaojuan
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Bioprocessing Innovative Company, 4734 Bridle Path Ct, Dublin, OH, 43017, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Lin
authors.affiliation: Bioprocessing Innovative Company, 4734 Bridle Path Ct, Dublin, OH, 43017, USA.
authors.forename: I-Ching
authors.initials: IC
authors.lastname: Tang
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000084:Acetate Kinase / Q000235:genetics / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000440:Butanols / Q000378:metabolism; D020148:Butyric Acid / Q000378:metabolism; D057465:Catabolite Repression; D003013:Clostridium / Q000235:genetics; D048014:Clostridium tyrobutyricum / Q000235:genetics* / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D000067030:Fruit and Vegetable Juices / Q000382:microbiology; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering; D031786:Saccharum / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
chemical_list: D000440:Butanols; D020148:Butyric Acid; D000431:Ethanol; D013395:Sucrose; D020001:1-Butanol; D000426:Alcohol Dehydrogenase; D000084:Acetate Kinase; D005947:Glucose
keywords: Butanol; Carbon catabolite repression; Clostridium tyrobutyricum; Metabolic engineering; Sucrose; Sugarcane juice