title: Patient-Derived and Intraoperatively Formed Biomaterial for Tissue Engineering.
abstract: In this chapter, we introduce a completely intraoperative procedure for obtaining a patient-derived biomaterial in cell therapy and tissue engineering applications. An automated device for processing human peripheral-blood ensures a reproducible method for retrieving the patient's cellular-rich as well as cellular-poor plasma. By substituting calcium for animal-derived thrombin, we engineer a completely autologous hydrogel that eliminates the risk of disease transmission and lowers FDA regulation hurdles. Through this chapter, we will discuss a bedside protocol developed to prepare a patient-derived hydrogel. This method can be effectively used to develop a completely intraoperative tissue engineering strategy (CITES) that can be easily translated into the clinic for surgical use.
authors.affiliation: Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Shalmli U
authors.initials: SU
authors.lastname: Joshi
authors.affiliation: Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Rares O
authors.initials: RO
authors.lastname: Barbu
authors.affiliation: Institute for Regenerative Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Carr-Reynolds
authors.affiliation: Arteriocyte, Cellular Therapies Medical Systems, Hopkinton, MA, 01748, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Barnes
authors.affiliation: Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA. syam@uchc.edu.|Institute for Regenerative Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA. syam@uchc.edu.|Materials Science & Engineering, University of Connecticut, Storrs, CT, 06269, USA. syam@uchc.edu.|Department of Orthopedic Surgery, University of Connecticut Health Center, Farmington, CT, 06030, USA. syam@uchc.edu.
authors.forename: Syam P
authors.initials: SP
authors.lastname: Nukavarapu
mesh_terms: D001672:Biocompatible Materials; D005337:Fibrin; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010949:Plasma; D023822:Tissue Engineering / Q000379:methods; D057188:Workflow
chemical_list: D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005337:Fibrin
keywords: Automated device; CITES; Cell therapy; Intraoperative; Peripheral blood; Tissue Engineering