title: Neodymium Oxide Induces Cytotoxicity and Activates NF-κB and Caspase-3 in NR8383 Cells.
abstract: We investigated whether Nd2O3 treatment results in cytotoxicity and other underlying effects in rat NR8383 alveolar macrophages. Cell viability assessed by the MTT assay revealed that Nd2O3 was toxic in a dose-dependent manner, but not in a time-dependent manner. An ELISA analysis indicated that exposure to Nd2O3 caused cell damage and enhanced synthesis and release of inflammatory chemokines. A Western blot analysis showed that protein expression levels of caspase-3, nuclear factor-κB (NF-κB) and its inhibitor IκB increased significantly in response to Nd2O3 treatment. Both NF-κB and caspase-3 signaling were activated, suggesting that both pathways are involved in Nd2O3 cytotoxicity.
authors.affiliation: School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, China.
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Huang
authors.affiliation: School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.affiliation: School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, China.
authors.forename: Yan Rong
authors.initials: YR
authors.lastname: Gao
authors.affiliation: School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, China.
authors.forename: Hai Nan
authors.initials: HN
authors.lastname: Xue
authors.affiliation: School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, China.
authors.forename: Su Hua
authors.initials: SH
authors.lastname: Wang
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000201:enzymology; D016328:NF-kappa B / Q000378:metabolism; D009354:Neodymium / Q000633:toxicity*; D010087:Oxides / Q000633:toxicity*; D051381:Rats; D018675:Toxicity Tests
chemical_list: D016328:NF-kappa B; D010087:Oxides; D009354:Neodymium; C505244:neodymium oxide; C505410:Casp3 protein, rat; D053148:Caspase 3