title: Primary Mouse Myoblast Purification using Magnetic Cell Separation.
abstract: Primary myoblasts can be isolated from mouse muscle cell extracts and cultured in vitro. Muscle cells are usually dissociated manually by mincing with razor blades or scissors in a collagenase/dispase solution. Primary myoblasts are then gradually enriched by pre-plating on collagen-coated plates, based on the observation that mouse fibroblasts attach quickly to collagen-coated plates, and are less adherent. Here, we describe an automated muscle dissociation protocol. We also propose an alternative to pre-plating using magnetic bead separation of primary myoblasts, which improve myoblast purity by minimizing fibroblast contamination.
authors.affiliation: Sprott Center For Stem Cell Research, Ottawa Hospital Research Institute, Regenerative Medicine Program, 501 Smyth, Box 511, Ottawa, ON, Canada, K1H 8L6.|Faculty of Medicine, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Marie Claude
authors.initials: MC
authors.lastname: Sincennes
authors.affiliation: Sprott Center For Stem Cell Research, Ottawa Hospital Research Institute, Regenerative Medicine Program, 501 Smyth, Box 511, Ottawa, ON, Canada, K1H 8L6.|Faculty of Medicine, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Yu Xin
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Sprott Center For Stem Cell Research, Ottawa Hospital Research Institute, Regenerative Medicine Program, 501 Smyth, Box 511, Ottawa, ON, Canada, K1H 8L6. mrudnicki@ohri.ca.|Faculty of Medicine, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada. mrudnicki@ohri.ca.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Rudnicki
mesh_terms: D000818:Animals; D015415:Biomarkers; D005434:Flow Cytometry / Q000379:methods; D018189:Immunomagnetic Separation / Q000379:methods*; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Fibroblast contamination; Magnetic separation; Magnetic-activated cell sorting; Muscle cells; Pre-plating; Primary myoblasts; Satellite cells