title: Next generation sequencing and molecular analysis of artichoke Italian latent virus.
abstract: Next-generation sequencing (NGS) allowed the assembly of the complete RNA-1 and RNA-2 sequences of a grapevine isolate of artichoke Italian latent virus (AILV). RNA-1 and RNA-2 are 7,338 and 4,630 nucleotides in length excluding the 3' terminal poly(A) tail, and encode two putative polyproteins of 255.8 kDa (p1) and 149.6 kDa (p2), respectively. All conserved motifs and predicted cleavage sites, typical for nepovirus polyproteins, were found in p1 and p2. AILV p1 and p2 share high amino acid identity with their homologues in beet ringspot virus (p1, 81% and p2, 71%), tomato black ring virus (p1, 79% and p2, 63%), grapevine Anatolian ringspot virus (p1, 65% and p2, 63%), and grapevine chrome mosaic virus (p1, 60% and p2, 54%), and to a lesser extent with other grapevine nepoviruses of subgroup A and C. Phylogenetic and sequence analyses, all confirmed the strict relationship of AILV with members classified in subgroup B of genus Nepovirus.
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, Valenzano, 70010, Bari, Italy. elbeaino@iamb.it.
authors.forename: Toufic
authors.identifier: http://orcid.org/0000-0003-2211-7907
authors.initials: T
authors.lastname: Elbeaino
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, Valenzano, 70010, Bari, Italy.
authors.forename: Imen
authors.initials: I
authors.lastname: Belghacem
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari, Via G. Amendola, 165/A, 70126, Bari, Italy.|Unità Operativa di Supporto di Bari, Istituto del CNR per la Protezione Sostenibile della Piante, Via G. Amendola 165/A, 70126, Bari, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Mascia
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari, Via G. Amendola, 165/A, 70126, Bari, Italy.|Unità Operativa di Supporto di Bari, Istituto del CNR per la Protezione Sostenibile della Piante, Via G. Amendola 165/A, 70126, Bari, Italy.
authors.forename: Donato
authors.initials: D
authors.lastname: Gallitelli
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, Valenzano, 70010, Bari, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Digiaro
mesh_terms: D000595:Amino Acid Sequence; D031943:Cynara scolymus / Q000821:virology; D059014:High-Throughput Nucleotide Sequencing; D007558:Italy; D017860:Nepovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D020815:Polyproteins / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D020815:Polyproteins