title: MicroRNA-3570 Modulates the NF-κB Pathway in Teleost Fish by Targeting MyD88.
abstract: The inflammatory response, a protective process to clear detrimental stimuli, constitutes the defense against infectious pathogens. However, excessive inflammation disrupts immune homeostasis, which may induce autoimmune and inflammatory diseases. In this study, we report that microRNA (miR)-3570 plays a negative role in the bacteria-induced inflammatory response of miiuy croaker. Upregulation of miR-3570 byVibrio anguillarumand LPS inhibits LPS-induced inflammatory cytokine production, thus avoiding an excessive inflammation response. Evidence showed that miR-3570 targets MyD88 and posttranscriptionally downregulates its expression. Overexpression of miR-3570 in macrophages suppresses the expression of MyD88, as well as its downstream signaling of IL-1R-associated kinases 1 and 4 and TNFR-associated factor 6. These results suggest that miR-3570 plays a regulatory in the bacteria-induced inflammatory response through the MyD88-mediated NF-κB signaling pathway by targeting MyD88.
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316022, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316022, China.
authors.forename: Yuena
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316022, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Cui
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316022, China tianjunxu@163.com.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D035683:MicroRNAs / Q000276:immunology* / Q000378:metabolism; D053594:Myeloid Differentiation Factor 88 / Q000276:immunology* / Q000378:metabolism; D016328:NF-kappa B / Q000276:immunology* / Q000378:metabolism; D010473:Perciformes; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology; D059467:Transcriptome; D014162:Transfection; D014733:Vibrio; D014735:Vibrio Infections / Q000276:immunology*
chemical_list: D035683:MicroRNAs; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B