title: Drosophila larvae food intake cessation following exposure to Erwinia contaminated media requires odor perception, Trpa1 channel and evf virulence factor.
abstract: When exposed to microorganisms, animals use several protective strategies. On one hand, as elegantly exemplified in Drosophila melanogaster, the innate immune system recognizes microbial compounds and triggers an antimicrobial response. On the other hand, behaviors preventing an extensive contact with the microbes and thus reducing the risk of infection have been described. However, these reactions ranging from microbes aversion to intestinal transit increase or food intake decrease have been rarely defined at the molecular level. In this study, we set up an experimental system that allowed us to rapidly identify and quantify food intake decreases in Drosophila larvae exposed to media contaminated with bacteria. Specifically, we report a robust dose-dependent food intake decrease following exposure to the bacteria Erwinia carotovora carotovora strain Ecc15. We demonstrate that this response does not require Imd innate immune pathway, but rather the olfactory neuronal circuitry, the Trpa1 receptor and the evf virulence factor. Finally, we show that Ecc15 induce the same behavior in the invasive pest insect Drosophila suzukii.
authors.affiliation: Aix-Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS), UMR 7288, Institut de Biologie du Développement de Marseille (IBDM), 13288 Marseille cedex 9, France.
authors.forename: Seydou
authors.initials: S
authors.lastname: Keita
authors.affiliation: Aix-Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS), UMR 7288, Institut de Biologie du Développement de Marseille (IBDM), 13288 Marseille cedex 9, France.
authors.forename: Ambra
authors.initials: A
authors.lastname: Masuzzo
authors.affiliation: Aix-Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS), UMR 7288, Institut de Biologie du Développement de Marseille (IBDM), 13288 Marseille cedex 9, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Royet
authors.affiliation: Aix-Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS), UMR 7288, Institut de Biologie du Développement de Marseille (IBDM), 13288 Marseille cedex 9, France. Electronic address: leopold.kurz@univ-amu.fr.
authors.forename: C Leopold
authors.initials: CL
authors.lastname: Kurz
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D004330:Drosophila / Q000276:immunology / Q000382:microbiology* / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics; D004435:Eating; D007113:Immunity, Innate; D007473:Ion Channels; D007814:Larva / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D055696:Olfactory Perception; D016973:Pectobacterium carotovorum / Q000235:genetics / Q000472:pathogenicity*; D000074025:TRPA1 Cation Channel / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D029721:Drosophila Proteins; D007473:Ion Channels; D000074025:TRPA1 Cation Channel; C475684:TrpA1 protein, Drosophila; D037521:Virulence Factors
keywords: Bacteria; Behavior; Drosophila; Food-intake; Olfaction