title: Assembled liposomes of dual podophyllotoxin phospholipid: preparation, characterization and in vivo anticancer activity.
abstract: OBJECTIVE
  A novel amphiphilic prodrug dual podophyllotoxin (PPT) succinate glycerophosphorylcholine (Di-PPT-GPC) assembled liposomes was developed to improve efficiency of PPT.
  
  
  METHODS
  Di-PPT-GPC liposomes were prepared by thin film technique and characterized by dynamic light scattering and cryo-electron microscopy.
  
  
  RESULTS
  In vitro release studies showed that Di-PPT-GPC liposomes could significantly release PPT in weakly acidic environment but had good stability under biological conditions. Methyl tetrazolium assay data revealed that the liposomes have comparable cytotoxicities to free PPT against MCF-7, HeLa and U87 cells. More importantly, in vivo antitumor evaluation indicated that Di-PPT-GPC liposomes exhibited favorable tumor growth inhibition without side effects.
  
  
  CONCLUSIONS
  Di-PPT-GPC liposomes might have potential to promote the therapeutic effect of PPT for cancer therapy.
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Longbing
authors.initials: L
authors.lastname: Ling
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yao
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Du
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ismail
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Ruiyu
authors.initials: R
authors.lastname: He
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Yongpeng
authors.initials: Y
authors.lastname: Hou
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Xinsong
authors.initials: X
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry; D061986:MCF-7 Cells; D009369:Neoplasms / Q000188:drug therapy*; D010743:Phospholipids / Q000008:administration & dosage* / Q000737:chemistry; D011034:Podophyllotoxin / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D010743:Phospholipids; D011034:Podophyllotoxin
keywords: anticancer activity; liposomes; podophyllotoxin; prodrug; self-assembly