title: Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine.
abstract: Heterotrophic bacterium, Enterobacter cloacae CF-S27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. With the initial nitrogen concentration of 100mgL-1h-1, ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2mgL-1h-1respectively. Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene (hao) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27. PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA. The strain Enterobacter cloacae CF-S27 significantly maintained the undetectable amount of dissolved nitrogen throughout 60days of zero water exchange fish culture experiment in domestic wastewater.
authors.affiliation: Environmental Microbiology, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Soumesh Kumar
authors.initials: SK
authors.lastname: Padhi
authors.affiliation: Environmental Microbiology, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Swetaleena
authors.initials: S
authors.lastname: Tripathy
authors.affiliation: Environmental Microbiology, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Sriprakash
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Environmental Microbiology, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Odisha, India. Electronic address: maitink@yahoo.co.in.
authors.forename: Nikhil Kumar
authors.initials: NK
authors.lastname: Maiti
mesh_terms: D000332:Aerobiosis; D064751:Ammonium Compounds / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D058440:Denitrification; D016972:Enterobacter cloacae / Q000378:metabolism*; D052836:Heterotrophic Processes; D019811:Hydroxylamine / Q000378:metabolism*; D009584:Nitrogen / Q000302:isolation & purification* / Q000493:pharmacokinetics*; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification / Q000379:methods
chemical_list: D064751:Ammonium Compounds; D019811:Hydroxylamine; D009584:Nitrogen; D062065:Wastewater
keywords: Aerobic denitrification; Enterobacter cloacae; Heterotrophic nitrification; Hydroxylamine reductase; qRT-PCR