title: Public health significance of major genotypes of Salmonella enterica serovar Enteritidis present in both human and chicken isolates in Korea.
abstract: Salmonella enterica serovar Enteritidis is one of the most common serotypes implicated in Salmonella infections in both humans and poultry worldwide. It has been reported that human salmonellosis is mainly associated with the consumption of poultry products contaminated with serovar Enteritidis. The present study was to extensively analyze the public health risk of serovar Enteritidis isolates from chickens in Korea. A total of 127 chicken isolates were collected from clinical cases, on-farm feces, and chicken meat between 1998 and 2012 and 20 human clinical isolates were obtained from patients with diarrhea between 2000 and 2006 in Korea. To characterize the isolates from chickens and humans, we compared the pulsed-field gel electrophoresis (PFGE) patterns and multilocus variable-number tandem-repeat analysis (MLVA) profiles of the isolates. We further characterized representative isolates of different genotypes using a DNA microarray. PFGE revealed 28 patterns and MLVA identified 16 allelic profiles. The DNA microarray showed high genetic variability in plasmid regions and other fimbrial subunits of the isolates although the virulence gene contents of isolates from the same source and/or of the same genotype were unrelated. PFGE and MLVA showed that major genotypes were present in both human and chicken isolates. This result suggests that chickens in Korea pose a significant risk to public health as a source of serovar Enteritidis as has been noted in other countries.
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea. Electronic address: kangmskr@korea.kr.
authors.forename: Min-Su
authors.initials: MS
authors.lastname: Kang
authors.affiliation: Department of Infectious Diseases & Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University, Iksan, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Oh
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea.
authors.forename: Yong-Kuk
authors.initials: YK
authors.lastname: Kwon
authors.affiliation: Division of Enteric Diseases, Center for Infectious Diseases, National Research Institute of Health, Cheongju, Republic of Korea.
authors.forename: Deog-Yong
authors.initials: DY
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea.
authors.forename: Ok-Mi
authors.initials: OM
authors.lastname: Jeong
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea.
authors.forename: Byung-Kook
authors.initials: BK
authors.lastname: Choi
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea.
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Youn
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea.
authors.forename: Byung-Woo
authors.initials: BW
authors.lastname: Jeon
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, Gimcheon-si, Gyeongsangbuk-do, Republic of Korea.
authors.forename: Hee-Soo
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces; D006801:Humans; D018598:Minisatellite Repeats; D011201:Poultry Diseases / Q000382:microbiology*; D011634:Public Health; D056910:Republic of Korea / Q000453:epidemiology; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics*
keywords: Chicken; Genotype; Human; MLVA; PFGE; Salmonella Enteritidis