title: Nanostructured polysaccharidic microcapsules for intracellular release of cisplatin.
abstract: Carbohydrate polimeric microcapsules were assembled using a LbL approach onto a CaCO3core. The microcapsules were used to delivery the anticancer drug cisplatin into HeLa and MCF-7 cancer cell lines. Drug encapsulation, measured by ICP spectroscopy, was around 50% of the charging solution. Fluorimetric measurements showed an efficient cellular uptake of polysacchardic microcapsules in both cell lines. The drug-loaded capsules demonstrated a better efficiency against cell viability than the free drug. Specifically, the amount of platinum reaching genomic DNA was measured, showing that encapsulation improves the nuclear delivery of the drug for both cell lines.
authors.affiliation: Biological and Environmental Sciences and Technology Department, University of Salento, & UdR INSTM di Lecce-Campus Universitario, via Monteroni, 73100 Lecce, Italy. Electronic address: viviana.vergaro@unisalento.it.
authors.forename: Viviana
authors.initials: V
authors.lastname: Vergaro
authors.affiliation: Biological and Environmental Sciences and Technology Department, University of Salento, & UdR INSTM di Lecce-Campus Universitario, via Monteroni, 73100 Lecce, Italy.
authors.forename: Paride
authors.initials: P
authors.lastname: Papadia
authors.affiliation: Dipartimento di Chimica, Materiali ed Ingegneria Chimica "G.Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Petrini
authors.affiliation: Biological and Environmental Sciences and Technology Department, University of Salento, & UdR INSTM di Lecce-Campus Universitario, via Monteroni, 73100 Lecce, Italy.
authors.forename: Francesco Paolo
authors.initials: FP
authors.lastname: Fanizzi
authors.affiliation: Biological and Environmental Sciences and Technology Department, University of Salento, & UdR INSTM di Lecce-Campus Universitario, via Monteroni, 73100 Lecce, Italy.
authors.forename: Sandra A
authors.initials: SA
authors.lastname: De Pascali
authors.affiliation: Biological and Environmental Sciences and Technology Department, University of Salento, & UdR INSTM di Lecce-Campus Universitario, via Monteroni, 73100 Lecce, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Baldassarre
authors.affiliation: Department of Informatics, Bioengineering, Robotics and Systems Engineering, Università degli Studi di Genova, Genoa, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Pastorino
authors.affiliation: Biological and Environmental Sciences and Technology Department, University of Salento, & UdR INSTM di Lecce-Campus Universitario, via Monteroni, 73100 Lecce, Italy; Istituto di Nanotecnologia (CNR-NANOTEC) University of Salento, Via Monteroni, 73100 Lecce, Italy. Electronic address: giuseppe.ciccarella@unisalento.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ciccarella
mesh_terms: D001692:Biological Transport; D002119:Calcium Carbonate / Q000737:chemistry; D002214:Capsules; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D061986:MCF-7 Cells; D049329:Nanostructures / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002214:Capsules; D004337:Drug Carriers; D011134:Polysaccharides; D002119:Calcium Carbonate; D002945:Cisplatin
keywords: Chitosan; Cisplatin; LbL; Pectin; Polysaccharidic microcapsules