title: Fast specific field detection of RHDVb.
abstract: This work describes a simple and rapid test for field detection of the emerging rabbit pathogen RHDVb. The assay is specific for RHDVb, showing no cross-reactivity with other RHDV types giving a specific result in under 10 min using rabbit liquid exudates or liver homogenate samples taken at necropsy.
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Edificio Santiago Gascón, Universidad de Oviedo, Campus El Cristo, Oviedo, Spain.
authors.forename: K P
authors.initials: KP
authors.lastname: Dalton
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Edificio Santiago Gascón, Universidad de Oviedo, Campus El Cristo, Oviedo, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Nicieza
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Edificio Santiago Gascón, Universidad de Oviedo, Campus El Cristo, Oviedo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Podadera
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Edificio Santiago Gascón, Universidad de Oviedo, Campus El Cristo, Oviedo, Spain.
authors.forename: D
authors.initials: D
authors.lastname: de Llano
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Edificio Santiago Gascón, Universidad de Oviedo, Campus El Cristo, Oviedo, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Martin Alonso
authors.affiliation: Area de Inmunología, Facultad de Medicina, Universidad de Oviedo, Oviedo, Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: de Los Toyos
authors.affiliation: Servicios Científicos Técnicos, Campus El Cristo, Universidad de Oviedo, Oviedo, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García Ocaña
authors.affiliation: Departamento de Cirugía, Facultad de Medicina, Universidad de Oviedo, Oviedo, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Vázquez-Villa
authors.affiliation: Certest Biotec S.L., San Mateo de Gállego, Zaragoza, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Velasco
authors.affiliation: Certest Biotec S.L., San Mateo de Gállego, Zaragoza, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Landeta
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Edificio Santiago Gascón, Universidad de Oviedo, Campus El Cristo, Oviedo, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Parra
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology*; D002846:Chromatography, Affinity / Q000662:veterinary; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D017929:Hemorrhagic Disease Virus, Rabbit / Q000302:isolation & purification*; D008807:Mice, Inbred BALB C; D011817:Rabbits / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral
keywords: RHDVb; calicivirus; diagnostics; emerging diseases; field detection; lagovirus; veterinary epidemiology