title: Surface induced dissociation as a tool to study membrane protein complexes.
abstract: Native ion mobility mass spectrometry (MS) and surface induced dissociation (SID) are applied to study the integral membrane protein complexes AmtB and AqpZ. Fragments produced from SID are consistent with the solved structures of these complexes. SID is, therefore, a promising tool for characterization of membrane protein complexes.
authors.affiliation: The Department of Chemistry and Biochemistry, The Ohio State University, 460 W 12th Avenue, Columbus, Ohio 43210, USA. wysocki.11@osu.edu and School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK.
authors.forename: Sophie R
authors.identifier: http://orcid.org/0000-0003-0763-8173
authors.initials: SR
authors.lastname: Harvey
authors.affiliation: Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, Texas 77030, USA. alaganowsky@ibt.tamhsc.edu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, Texas 77030, USA. alaganowsky@ibt.tamhsc.edu.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: The Department of Chemistry and Biochemistry, The Ohio State University, 460 W 12th Avenue, Columbus, Ohio 43210, USA. wysocki.11@osu.edu.
authors.forename: Vicki H
authors.initials: VH
authors.lastname: Wysocki
authors.affiliation: Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, Texas 77030, USA. alaganowsky@ibt.tamhsc.edu and Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Arthur
authors.initials: A
authors.lastname: Laganowsky
mesh_terms: D020346:Aquaporins / Q000737:chemistry*; D027682:Cation Transport Proteins / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: C111096:AmtB protein, E coli; D020346:Aquaporins; D027682:Cation Transport Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C106425:aqpZ protein, E coli