title: Wound Myiasis Caused bySarcophaga (Liopygia) Argyrostoma(Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation.
abstract: In Mediterranean countries,Sarcophaga (Liopygia) crassipalpis,Sarcophaga (L.) argyrostoma,andSarcophaga (L.) cultellatashare the same ecological niche and can be responsible of myiasis. In this study, the main morphological characters of a larva found in a hospitalized woman were described and illustrated by light and SEM microscopy and the features discussed. Then, a fragment within the mitochondrial encoded cytochrome c oxidase subunit I(coxI)gene of ~735 bp was amplified and sequenced. The molecular investigation was necessary to confirm the speciesSarcophaga (Liopygia) argyrostoma(99% of identity). Our findings showed that morphological descriptions of larvae of three Mediterranean species ofLiopygiaavailable in several papers might not be clear enough to allow for comparison and correct identification. Until results of reliable comparative studies of larvae of all three species will be available, the use of molecular tools is crucial, to avoid misleading or incomplete identification, and in particular when a myiasis becomes a legal issue.
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli 25, Foggia, Italy.
authors.forename: Annunziata
authors.identifier: 0000-0002-3357-8672
authors.initials: A
authors.lastname: Giangaspero
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli 25, Foggia, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Marangi
authors.affiliation: Unità Operativa di Geriatria, Ospedale M. Bufalini, AUSL della Romagna, Ravenna, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Balotta
authors.affiliation: Dipartimento di Sanità Pubblica, AUSL della Romagna, Ravenna, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Venturelli
authors.affiliation: Chair of Ecology and Biogeography, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szpila
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli 25, Foggia, Italy.
authors.forename: Antonella
authors.identifier: 0000-0002-9502-9820
authors.initials: A
authors.lastname: Di Palma
mesh_terms: D000818:Animals; D051545:Cyclooxygenase 1 / Q000235:genetics; D005260:Female; D017344:Genes, Insect; D006801:Humans; D007814:Larva / Q000033:anatomy & histology / Q000145:classification; D009198:Myiasis / Q000473:pathology*; D058493:Sarcophagidae / Q000033:anatomy & histology / Q000145:classification*
chemical_list: D051545:Cyclooxygenase 1