title: Construction of a Chassis for a Tripartite Protein-Based Molecular Motor.
abstract: Improving our understanding of biological motors, both to fully comprehend their activities in vital processes, and to exploit their impressive abilities for use in bionanotechnology, is highly desirable. One means of understanding these systems is through the production of synthetic molecular motors. We demonstrate the use of orthogonal coiled-coil dimers (including both parallel and antiparallel coiled coils) as a hub for linking other components of a previously described synthetic molecular motor, the Tumbleweed. We use circular dichroism, analytical ultracentrifugation, dynamic light scattering, and disulfide rearrangement studies to demonstrate the ability of this six-peptide set to form the structure designed for the Tumbleweed motor. The successful formation of a suitable hub structure is both a test of the transferability of design rules for protein folding as well as an important step in the production of a synthetic protein-based molecular motor.
authors.affiliation: Department of Physics, Durham University , Durham, DH1 3LE, United Kingdom.
authors.forename: Lara S R
authors.initials: LSR
authors.lastname: Small
authors.affiliation: School of Chemistry, University of Bristol , BS8 1TS, Bristol, United Kingdom.
authors.forename: Marc
authors.initials: M
authors.lastname: Bruning
authors.affiliation: School of Chemistry, University of Glasgow , Glasgow, G12 8QQ, United Kingdom.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Thomson
authors.affiliation: Faculty of Science, Leiden Institute of Chemistry , Leiden, 2333 CC, Netherlands.
authors.forename: Aimee L
authors.initials: AL
authors.lastname: Boyle
authors.affiliation: Structural Biology Laboratory, Victor Chang Cardiac Research Institute , Darlinghurst, New South Wales 2010, Australia.
authors.forename: Roberta B
authors.initials: RB
authors.lastname: Davies
authors.affiliation: School of Physics, University of New South Wales , Sydney, New South Wales 2052, Australia.
authors.forename: Paul M G
authors.initials: PMG
authors.lastname: Curmi
authors.affiliation: Department of Physics, Simon Fraser University , Burnaby, British Columbia V5A 1S6, Canada.
authors.forename: Nancy R
authors.initials: NR
authors.lastname: Forde
authors.affiliation: NanoLund and Solid State Physics, Lund University , Box 118, 22100 Lund, Sweden.
authors.forename: Heiner
authors.initials: H
authors.lastname: Linke
authors.affiliation: School of Chemistry, University of Bristol , BS8 1TS, Bristol, United Kingdom.|School of Biochemistry, University of Bristol , BS8 1TD, Bristol, United Kingdom.|BrisSynBio, Life Sciences Building, University of Bristol , BS8 1TQ, Bristol, United Kingdom.
authors.forename: Derek N
authors.identifier: 0000-0002-0394-3202
authors.initials: DN
authors.lastname: Woolfson
authors.affiliation: Department of Physics, Durham University , Durham, DH1 3LE, United Kingdom.
authors.forename: Elizabeth H C
authors.identifier: 0000-0002-7678-1187
authors.initials: EHC
authors.lastname: Bromley
mesh_terms: D002942:Circular Dichroism; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D020409:Molecular Motor Proteins; D010455:Peptides; D021122:Protein Subunits