title: HSP70 expression in Biomphalaria glabrata snails exposed to cadmium.
abstract: In this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96h to CdCl2at concentrations ranging from 0.09 to 0.7mgL-1(LC50/96h=0.34 (0.30-0.37). Time and concentration-dependent increases in the expression of HSP70 were observed at sub-lethal levels in the immunoblotting assay. Further, an increased survival to a lethal heat shock was observed in animals pre-exposed to a nonlethal concentration of cadmium, evidencing the induction of acquired tolerance. The present study demonstrated the inducibility of B. glabrata HSP70 by cadmium, a relevant environmental contaminant, at non-lethal levels, providing evidences that the assessment of HSP70 in B. glabrata can be regarded as a suitable biomarker for ecotoxicological studies.
authors.affiliation: Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil; Instituto de Pesquisas Energéticas e Nucleares, Avenida Professor Lineu Prestes, 2242, Cidade Universitária, São Paulo, SP CEP 05508-000, Brazil. Electronic address: rcantinha@gmail.com.
authors.forename: Rebeca
authors.initials: R
authors.lastname: da Silva Cantinha
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares, Avenida Professor Lineu Prestes, 2242, Cidade Universitária, São Paulo, SP CEP 05508-000, Brazil. Electronic address: sborrely@ipen.br.
authors.forename: Sueli Ivone
authors.initials: SI
authors.lastname: Borrely
authors.affiliation: Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil. Electronic address: nancyoguiura@butantan.gov.br.
authors.forename: Nancy
authors.initials: N
authors.lastname: Oguiura
authors.affiliation: Departamento de Estatística, Instituto de Matemática e Estatística, Universidade de São Paulo, Rua do Matão, 1010, Cidade Universitária, São Paulo, SP CEP 05008-090, Brazil. Electronic address: cadebp@gmail.com.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: de Bragança Pereira
authors.affiliation: Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil. Electronic address: marcelarigolon@hotmail.com.
authors.forename: Marcela M
authors.initials: MM
authors.lastname: Rigolon
authors.affiliation: Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil. Electronic address: eliana.nakano@butantan.gov.br.
authors.forename: Eliana
authors.initials: E
authors.lastname: Nakano
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000187:drug effects* / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance; D054750:Ecotoxicology; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D018384:Oxidative Stress / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D002104:Cadmium
keywords: Acquired resistance; Bioindicator; Biomarker; Mollusk; Protein induction