title: The renal consequences of maternal obesity in offspring are overwhelmed by postnatal high fat diet.
abstract: OBJECTIVE
  Developmental programming induced by maternal obesity influences the development of chronic disease in offspring. In the present study, we aimed to determine whether maternal obesity exaggerates obesity-related kidney disease.
  
  
  METHODS
  Female C57BL/6 mice were fed high-fat diet (HFD) for six weeks prior to mating, during gestation and lactation. Male offspring were weaned to normal chow or HFD. At postnatal Week 8, HFD-fed offspring were administered one dose streptozotocin (STZ, 100 mg/kg i.p.) or vehicle control. Metabolic parameters and renal functional and structural changes were observed at postnatal Week 32.
  
  
  RESULTS
  HFD-fed offspring had increased adiposity, glucose intolerance and hyperlipidaemia, associated with increased albuminuria and serum creatinine levels. Their kidneys displayed structural changes with increased levels of fibrotic, inflammatory and oxidative stress markers. STZ administration did not potentiate the renal effects of HFD. Though maternal obesity had a sustained effect on serum creatinine and oxidative stress markers in lean offspring, the renal consequences of maternal obesity were overwhelmed by the powerful effect of diet-induced obesity.
  
  
  CONCLUSIONS
  Maternal obesity portends significant risks for metabolic and renal health in adult offspring. However, diet-induced obesity is an overwhelming and potent stimulus for the development of CKD that is not potentiated by maternal obesity.
authors.affiliation: Department of Medicine, Kolling Institute, University of Sydney, Sydney, Australia.|Department of Diabetes, Endocrinology and Metabolism, Royal North Shore Hospital, St Leonards, NSW, Australia.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Glastras
authors.affiliation: School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, Australia.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Tsang
authors.affiliation: Department of Medicine, Kolling Institute, University of Sydney, Sydney, Australia.
authors.forename: Rachel
authors.initials: R
authors.lastname: Teh
authors.affiliation: Department of Medicine, Kolling Institute, University of Sydney, Sydney, Australia.|Department of Diabetes, Endocrinology and Metabolism, Royal North Shore Hospital, St Leonards, NSW, Australia.
authors.forename: Rachel T
authors.initials: RT
authors.lastname: McGrath
authors.affiliation: Department of Medicine, Kolling Institute, University of Sydney, Sydney, Australia.
authors.forename: Amgad
authors.initials: A
authors.lastname: Zaky
authors.affiliation: Department of Anatomical Pathology, Royal North Shore Hospital, St Leonards, NSW, Australia.
authors.forename: Jason
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Medicine, Kolling Institute, University of Sydney, Sydney, Australia.
authors.forename: Muh Geot
authors.initials: MG
authors.lastname: Wong
authors.affiliation: Department of Medicine, Kolling Institute, University of Sydney, Sydney, Australia.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Pollock
authors.affiliation: Department of Medicine, Kolling Institute, University of Sydney, Sydney, Australia.
authors.forename: Sonia
authors.initials: S
authors.lastname: Saad
mesh_terms: D050154:Adiposity / Q000502:physiology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D059305:Diet, High-Fat; D005260:Female; D005355:Fibrosis / Q000378:metabolism / Q000473:pathology; D018149:Glucose Intolerance / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers; D003404:Creatinine