title: Cryptomphalus aspersa Mollusc Egg Extract Promotes Regenerative Effects in Human Dermal Papilla Stem Cells.
abstract: The aim of this study was to test, by an in vitro approach, whether a natural extract derived from eggs of the molluscCryptomphalus aspersa(e-CAF) that seems to present regenerative properties, can enhance the mobilization of human hair dermal papilla cells (HHDPCs) and play a role on tissue repair and regeneration. We have tested HHDPCs proliferation by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium-bromide (MTT) assay; cell migration by using a wound healing assay, as well as the modulation of the expression of cytoskeletal (F-actin and vimentin) and cell adhesion to the extracellular matrix (ECM) (vinculin and P-FAK) proteins. We also explored whether e-CAF could lead HHDPCs to keratinocytes and/or fibroblasts by evaluating the expression of specific markers. We have compared these e-CAF effects with those induced by TGFβ₁, implicated in regulation of cell proliferation and migration. e-CAF promotes proliferation and migration of HDDPCs cells in a time- and dose-dependent manner; it also increases the migratory behavior and the expression of adhesion molecules. These results support the fact that e-CAF could play a role on skin regeneration and be used for the prevention or repair of damaged tissue, either due to external causes or as a result of cutaneous aging.
authors.affiliation: Department of Biology, Faculty of Sciences, Universidad Autónoma de Madrid, Darwin, 2, 28049 Madrid, Spain. maria.alameda@uam.es.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Alameda
authors.affiliation: Department of Biology, Faculty of Sciences, Universidad Autónoma de Madrid, Darwin, 2, 28049 Madrid, Spain. morelesther@yahoo.es.
authors.forename: Esther
authors.initials: E
authors.lastname: Morel
authors.affiliation: Department of Pathology, Faculty of Medicine, Universidad de Málaga, Avda. Cervantes, 2, 29071 Málaga, Spain. cparrado@uma.es.
authors.forename: Concepción
authors.initials: C
authors.lastname: Parrado
authors.affiliation: Dermatology Service, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA. gonzals6@mskcc.org.|Department of Medicine and Medical Specialties, Universidad de Alcalá, 28805 Madrid, Spain. gonzals6@mskcc.org.
authors.forename: Salvador
authors.initials: S
authors.lastname: González
authors.affiliation: Department of Biology, Faculty of Sciences, Universidad Autónoma de Madrid, Darwin, 2, 28049 Madrid, Spain. angeles.juarranz@uam.es.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Juarranz
mesh_terms: D000818:Animals; D001688:Biological Products / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003599:Cytoskeleton / Q000378:metabolism; D020405:Dermis / Q000166:cytology* / Q000502:physiology*; D049851:Gastropoda / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects*; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001688:Biological Products
keywords: Cryptomphalus aspersa; cell adhesion; cell differentiation; fibroblasts; human dermal papilla cells; proliferation