title: Structural Basis of the Substrate Specificity and Enzyme Catalysis of aPapaver somniferumTyrosine Decarboxylase.
abstract: Tyrosine decarboxylase (TyDC), a type II pyridoxal 5'-phosphate decarboxylase, catalyzes the decarboxylation of tyrosine. Due to a generally high sequence identity to other aromatic amino acid decarboxylases (AAADs), primary sequence information is not enough to understand substrate specificities with structural information. In this study, we selected a typical TyDC fromPapaver somniferumas a model to study the structural basis of AAAD substrate specificities. Analysis of the nativeP. somniferumTyDC crystal structure and subsequent molecular docking and dynamics simulation provide some structural bases that explain substrate specificity for tyrosine. The result confirmed the previous proposed mechanism for the enzyme selectivity of indolic and phenolic substrates. Additionally, this study yields the first crystal structure for a plant type II pyridoxal-5'-phosphate decarboxylase.
authors.affiliation: Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan UniversityHainan, China; Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityHainan, China; Laboratory of Tropical Veterinary Medicine and Vector Biology, Hainan UniversityHaikou, Hainan, China.
authors.forename: Huai
authors.initials: H
authors.lastname: Guan
authors.affiliation: Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan UniversityHainan, China; Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityHainan, China; Laboratory of Tropical Veterinary Medicine and Vector Biology, Hainan UniversityHaikou, Hainan, China.
authors.forename: Shuaibao
authors.initials: S
authors.lastname: Song
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton New York, NY, USA.
authors.forename: Howard
authors.initials: H
authors.lastname: Robinson
authors.affiliation: Department of Biochemistry, Virginia Tech Blacksburg, VA, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Biochemistry, Virginia Tech Blacksburg, VA, USA.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Biochemistry, Virginia Tech Blacksburg, VA, USA.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan UniversityHainan, China; Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityHainan, China; Laboratory of Tropical Veterinary Medicine and Vector Biology, Hainan UniversityHaikou, Hainan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Han
keywords: Papaver somniferum; aromatic amino acid decarboxylase; crystal structure; decarboxylase; substrate specificity; tyrosine decarboxylases