title: A Novel Monoclonal Antibody Against Alpha-Methylacyl-CoA Racemase Applicable for Paraffin-Embedded Tissues and Diagnostics of Prostate Cancer.
abstract: AMACR (alpha-methylacyl-CoA racemase) has been recently described as a prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma, but not in benign prostatic tissue. Thus, monoclonal antibodies (mAbs) for AMACR detection are an important tool for the diagnosis of AMACR-positive cancers. However, only a few mAbs, especially those applicable for immunohistochemistry (IHC), have been established to date. In this study, we describe the generation of a new hybridoma clone G8 producing anti-AMACR antibodies. G8 mAb specifically binds human AMACR and was successfully used in immunoblotting and immunofluorescence on paraformaldehyde-fixed cells and in IHC of paraffin-embedded tumor specimens. These results indicate that this new anti-AMACR mAb G8 would be useful in the diagnosis of AMACR-related cancers and would be a strong tool in both basic and clinical research on AMACR.
authors.affiliation: 1 Institute of Carcinogenesis , NN Blokhin Russian Cancer Research Center, Moscow, Russia .
authors.forename: Olga V
authors.initials: OV
authors.lastname: Kovaleva
authors.affiliation: 1 Institute of Carcinogenesis , NN Blokhin Russian Cancer Research Center, Moscow, Russia .
authors.forename: Daria V
authors.initials: DV
authors.lastname: Samoilova
authors.affiliation: 1 Institute of Carcinogenesis , NN Blokhin Russian Cancer Research Center, Moscow, Russia .
authors.forename: Maria S
authors.initials: MS
authors.lastname: Shitova
authors.affiliation: 2 Lomonosov Moscow State University , Moscow, Russia .
authors.forename: Nina A
authors.initials: NA
authors.lastname: Oleinikova
authors.affiliation: 2 Lomonosov Moscow State University , Moscow, Russia .
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Danilova
authors.affiliation: 2 Lomonosov Moscow State University , Moscow, Russia .
authors.forename: Pavel G
authors.initials: PG
authors.lastname: Malkov
authors.affiliation: 1 Institute of Carcinogenesis , NN Blokhin Russian Cancer Research Center, Moscow, Russia .|3 Medical Faculty Mannheim, Ruprecht-Karls University of Heidelberg , Mannheim, Germany .
authors.forename: Alexei
authors.initials: A
authors.lastname: Gratchev
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology*; D019998:Racemases and Epimerases / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase
keywords: AMACR; carcinoma; prostate