title: A ThDREB gene from Tamarix hispida improved the salt and drought tolerance of transgenic tobacco and T. hispida.
abstract: Dehydration-responsive element-binding (DREB) transcription factors are important abiotic stress tolerance related genes, and some reports on the roles of DREB have primarily addressed herbal plants. To explore the abiotic stress tolerance role of DREB (ThDREB) from Tamarix hispida, a ThDREB gene with a complete ORF of 783 bp that encodes a 28.74 kDa protein with 260 amino acids, was isolated and functionally annotated. ThDREB expression was highly induced by NaCl, PEG, NaHCO3and CdCl2treatments, and the highest expression level (369.2-fold of control) was found for the roots that were under NaCl stress for 6 h. The tobacco plants that were transformed by ThDREB were conferred with higher germination rates, fresh weights and root lengths than the wild type (WT) tobacco plants under NaCl and mannitol treatments. The total chlorophyll content (tcc), superoxide dismutase (SOD) and peroxidase (POD) activities were also higher in the transgenic lines in comparison with the WT, and the malondialdehyde (MDA) and H2O2content, electrolyte leakage (EL) rate and ROS as tracked by staining were generated to a lesser degree in ThDREB transgenic plants than in the WT under NaCl and mannitol stress. Furthermore, the transient overexpression analysis of ThDREB in T. hispida also improved plant salt and drought tolerance in comparison with the empty vector-transformed lines. Our results indicated that ThDREB expression could effectively improve tolerance to salt and drought stress by enhancing the antioxidase activity that keeps the ROS at a low accumulation level and makes them easy to scavenge.
authors.affiliation: Laboratory of Walnut Research Center, College of Forestry, Northwest A & F University, Yangling 712100, Shaanxi, China; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Guiyan
authors.initials: G
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Kaimin
authors.initials: K
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Yucong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China. Electronic address: gaocaiqiu@yahoo.com.
authors.forename: Caiqiu
authors.initials: C
authors.lastname: Gao
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D019256:Cadmium Chloride / Q000494:pharmacology; D002734:Chlorophyll / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011092:Polyethylene Glycols / Q000494:pharmacology; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism; D032363:Tamaricaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; C000595215:Polyethylene Glycol 6000; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D008315:Malondialdehyde; D017693:Sodium Bicarbonate; D006861:Hydrogen Peroxide; D009195:Peroxidase; D013482:Superoxide Dismutase; D019256:Cadmium Chloride
keywords: Abiotic stress; DREB; ROS metabolism; Salt and drought tolerance; Tamarix hispida