title: [Over-expression of C3AR1 Promotes HL-60 Cell Migration and Invasion].
abstract: OBJECTIVE
  To investigate the effect of C3AR1 on migration and invasion of HL-60 cells and its mechanism.
  
  
  METHODS
  The HL-60 cells overexpresing C3AR1 was constructed, and the HL-60-C3AR1 cell line was validated by real-time PCR and Western blot. The migration and invasion assay were performed by using transwell system, the PCR Array and flow cytometry were employed to reveal the potential mechanisms.
  
  
  RESULTS
  C3AR1 gene was significantly expressed in AL cell lines, especially in acute myeloid leukemia(AML cell lines). C3a and SDF-1 together promoted migration and invasion of C3AR1 over-expressed HL-60 cells. The PCR array detection found that 16 genes (including CCL2) were significantly upregulated and 4 genes were significantly down-regulated. However, the expression of CXCR4 did not significantly change after treated by C3a and SDF-1 together.
  
  
  CONCLUSIONS
  Over-expression of C3AR1 shows the effects on the migration and invasion of HL-60 cells under the treatment of C3a and SDF-1 together, which may be mediated by the regulation of CCL2 and other genes related to chemotactic factors.
authors.affiliation: Department of Hematology, The Affiliated Children's Hospital of Soochow University,Suzhou 215000, Jiangsu Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Department of Hematology, The Affiliated Children's Hospital of Soochow University,Suzhou 215000, Jiangsu Province, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China.
authors.forename: Jian-Nong
authors.initials: JN
authors.lastname: Cen
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China.
authors.forename: Su-Ning
authors.initials: SN
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Affiliated Children's Hospital of Soochow University,Suzhou 215000, Jiangsu Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Hematology, The Affiliated Children's Hospital of Soochow University,Suzhou 215000, Jiangsu Province, China. E-mail: hsy139@126.com.
authors.forename: Shao-Yan
authors.initials: SY
authors.lastname: Hu
mesh_terms: D002465:Cell Movement; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D011951:Receptors, Complement / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D011951:Receptors, Complement; C085567:complement C3a receptor