title: Identification of miR-146b-5p in tissues as a novel biomarker for prognosis of gallbladder carcinoma.
abstract: Increasing evidence has suggested that dysregulation of microRNAs (miRNAs) could contribute to tumor progression. The aim of present study was to evaluate the feasibility of using miR-146b-5p as a prognostic biomarker in gallbladder cancer (GBC).
  
  
  
  We collected 150 pairs of tissue specimens from patients with GBC and adjacent normal specimens. The expression level of miR-146b-5p was measured in 150 GBC tissues and adjacent normal tissues RT-qPCR. Kaplan-Meier method was used to analyze the overall survival. The univariate and multivariable Cox regression analyses were performed to identify whether miR-146b-5p could serve as an independent prognostic factor for GBC patients.
  
  
  
  miR-146b-5p expression was decreased in GBC tissues compared with that in adjacent normal tissues (p < 0.01). In addition, miR-146b-5p expression was correlated with TNM stage (p = 0.009), liver metastasis (p = 0.001) and differentiated degree (p = 0.022). Kaplan-Meier survival curves showed that GBC patients with high miR-146b-5p expression showed better overall survival than those with low miR-146b-5p expression (p = 0.0005). At last, univariate and multivariate analyses confirmed that increased miR-146b-5p expression was an independent predictive factor of good prognosis for GBC patients.
  
  
  
  Our study showed that miR-146b-5p was a potential prognostic biomarker and higher expression of miR-146b-5p is associated with a poor prognosis in GBC.
authors.affiliation: Department of Internal Medicine of Dahua Branch Hospital, Medical Statistics Research Center, Department of Ultrasound, Central Laboratory, Department of Oncological Surgery; Cangzhou Central Hospital, Cangzhou, Hebei, China. vv8617@yeah.net.
authors.forename: Y-P
authors.initials: YP
authors.lastname: Lv
authors.forename: W
authors.initials: W
authors.lastname: Shi
authors.forename: H-X
authors.initials: HX
authors.lastname: Liu
authors.forename: X-J
authors.initials: XJ
authors.lastname: Kong
authors.forename: D-L
authors.initials: DL
authors.lastname: Dai
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D005260:Female; D005706:Gallbladder Neoplasms / Q000175:diagnosis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C542542:MIRN146 microRNA, human; D035683:MicroRNAs