title: [Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation].
abstract: OBJECTIVE
  To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation.
  
  
  METHODS
  Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA.
  
  
  RESULTS
  The expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor (LIFR) expression increased progressively during adipogenic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p.
  
  
  CONCLUSIONS
  miRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.
authors.affiliation: 1Key Laboratory of Jiangxi Province for Systems Biomedicine, Jiujiang 332000, China; 2Department of Pathogenic Biology, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, China. E-mail: comwangtaocom@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Rui-Qiao
authors.initials: RQ
authors.lastname: Yan
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Cao
authors.forename: Xuan-Pu
authors.initials: XP
authors.lastname: Zhang
authors.forename: Xing-Nuan
authors.initials: XN
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Xiao-Ou
authors.initials: XO
authors.lastname: Zhou
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Wu
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Xu
mesh_terms: D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology; D012333:RNA, Messenger; D059467:Transcriptome
chemical_list: C507840:LIFR protein, human; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D012333:RNA, Messenger