title: Enzyme-Controlled Nanodevice for Acetylcholine-Triggered Cargo Delivery Based on Janus Au-Mesoporous Silica Nanoparticles.
abstract: This work reports a new gated nanodevice for acetylcholine-triggered cargo delivery. We prepared and characterized Janus Au-mesoporous silica nanoparticles functionalized with acetylcholinesterase on the Au face and with supramolecular β-cyclodextrin:benzimidazole inclusion complexes as caps on the mesoporous silica face. The nanodevice is able to selectively deliver the cargo in the presence of acetylcholine via enzyme-mediated acetylcholine hydrolysis, locally lowering the pH and opening the supramolecular gate. Given the key role played by ACh and its relation with Parkinson's disease and other nervous system diseases, we believe that these findings could help design new therapeutic strategies.
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universidat Politécnica de Valencia, Universitat de Valencia, Spain.|Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Llopis-Lorente
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universidat Politécnica de Valencia, Universitat de Valencia, Spain.|Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: de la Torre
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.|IMDEA Nanoscience Cantoblanco University City, 28049, Madrid, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sánchez
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universidat Politécnica de Valencia, Universitat de Valencia, Spain.|Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: Sancenón
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universidat Politécnica de Valencia, Universitat de Valencia, Spain.|Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Aznar
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universidat Politécnica de Valencia, Universitat de Valencia, Spain.|Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: María D
authors.initials: MD
authors.lastname: Marcos
authors.affiliation: Department of Organic Chemistry I, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Martínez-Ruíz
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universidat Politécnica de Valencia, Universitat de Valencia, Spain.|Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Martínez-Máñez
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.|IMDEA Nanoscience Cantoblanco University City, 28049, Madrid, Spain.
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D001562:Benzimidazoles / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D053758:Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D001562:Benzimidazoles; D004337:Drug Carriers; D004800:Enzymes, Immobilized; C509968:MCM-41; D009942:Organometallic Compounds; D047392:beta-Cyclodextrins; C415768:tris(2,2'-bipyridyl)ruthenium(II); D006046:Gold; D012822:Silicon Dioxide; D004317:Doxorubicin; C031000:benzimidazole; D000110:Acetylcholinesterase; C031215:betadex; D000109:Acetylcholine
keywords: acetylcholine; controlled release; mesoporous materials; nanoparticles; nanotechnology