title: Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model.
abstract: The previously developed gelatin/silk fibroin microspheres were loaded with curcumin and applied for anti-inflammatory treatment in monosodium iodoacetate (MIA)-induced osteoarthritis (OA) in a rat model. The MIA-induced OA rats received a single intra-articular injection with gelatin or gelatin/silk fibroin (30/70) microspheres encapsulating curcumin. The therapeutic effects of treatment groups [concentration of interleukin-6 (IL-6) in blood serum, radiographic and the histological grading on articular joint] were compared with those of normal saline treated OA and normal rats. The result showed that both microsphere groups reduced the level of IL-6 in serum after 1 week of treatment. The gelatin/silk fibroin (30/70) microspheres encapsulating curcumin delayed the cellular destruction in articular joint and synovial tissue after 8 weeks. The radiographic and histological gradings on articular cartilage lesion and synovial tissue change of rats treated with gelatin/silk fibroin (30/70) microspheres encapsulating curcumin were close to those of the normal rats. It was explained that the slow-degrading gelatin/silk fibroin (30/70) microspheres released curcumin for extended period and showed a prolonged anti-inflammatory effect, compared to the fast-degrading gelatin microspheres. This delivery system of curcumin was suggested to be applied for localized treatment of anti-inflammatory in OA with minimal invasion.
authors.affiliation: Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, PhayaThai Road, Bangkok, 10330, Thailand.
authors.forename: Juthamas
authors.initials: J
authors.lastname: Ratanavaraporn
authors.affiliation: Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, PhayaThai Road, Bangkok, 10330, Thailand.
authors.forename: Kumpanart
authors.initials: K
authors.lastname: Soontornvipart
authors.affiliation: Department of Medical Services, Institute of Pathology, Ministry of Public Health, Yothi Road, Bangkok, 10400, Thailand.
authors.forename: Somruetai
authors.initials: S
authors.lastname: Shuangshoti
authors.affiliation: Department of Pathology and Chulalongkorn GenePRO Center, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Bangkok, 10330, Thailand.
authors.forename: Shanop
authors.initials: S
authors.lastname: Shuangshoti
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, PhayaThai Road, Bangkok, 10330, Thailand. siriporn.d@chula.ac.th.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Damrongsakkul
keywords: Controlled release; Curcumin; Gelatin; Microsphere; Osteoarthritis; Silk fibroin