title: Isolation of Mouse Stromal Vascular Cells for Monolayer Culture.
abstract: Positive energy balance contributes to adipose tissue expansion and dysfunction, which accounts largely for obesity and related metabolic disorders. Thermogenic fat can dissipate energy, activation or induction of which may promote energy balance and address the pressing health issues. Recent studies have shown that stromal vascular fraction (SVF) from white adipose tissue (WAT) can develop both white and brown-like adipocyte phenotypes, thus serving as a unique model to study adipogenesis and thermogenesis. Here, we describe a protocol for effective isolation of mouse SVF from WAT, induction of differentiation, and detection of adipogenesis. Success tips for isolation and culture of SVF are also discussed.
authors.affiliation: Department of Human Nutrition, Foods and Exercise, Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA.
authors.forename: Longhua
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Human Nutrition, Foods and Exercise, Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA.
authors.forename: Louise D
authors.initials: LD
authors.lastname: Zheng
authors.affiliation: Department of Human Nutrition, Foods and Exercise, Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA.
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Donnelly
authors.affiliation: Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Margo P
authors.initials: MP
authors.lastname: Emont
authors.affiliation: Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Human Nutrition, Foods and Exercise, Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA. zcheng@vt.edu.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D050156:Adipogenesis; D052436:Adipose Tissue, White / Q000166:cytology*; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D004734:Energy Metabolism; D050356:Lipid Metabolism; D051379:Mice; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism
keywords: Adipogenesis; Adipose tissue; Energy balance; Lipid accumulation; Obesity; Stromal vascular fraction