title: Diverse Central Projection Patterns of Retinal Ganglion Cells.
abstract: Understanding how >30 types of retinal ganglion cells (RGCs) in the mouse retina each contribute to visual processing in the brain will require more tools that label and manipulate specific RGCs. We screened and analyzed retinal expression of Cre recombinase using 88 transgenic driver lines. In many lines, Cre was expressed in multiple RGC types and retinal cell classes, but several exhibited more selective expression. We comprehensively mapped central projections from RGCs labeled in 26 Cre lines using viral tracers, high-throughput imaging, and a data processing pipeline. We identified over 50 retinorecipient regions and present a quantitative retina-to-brain connectivity map, enabling comparisons of target-specificity across lines. Projections to two major central targets were notably correlated: RGCs projecting to the outer shell or core regions of the lateral geniculate projected to superficial or deep layers within the superior colliculus, respectively. Retinal images and projection data are available online at http://connectivity.brain-map.org.
authors.affiliation: Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Emily M
authors.initials: EM
authors.lastname: Martersteck
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Karla E
authors.initials: KE
authors.lastname: Hirokawa
authors.affiliation: Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Mariah
authors.initials: M
authors.lastname: Evarts
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Bernard
authors.affiliation: Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Duan
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Lydia
authors.initials: L
authors.lastname: Ng
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Seung W
authors.initials: SW
authors.lastname: Oh
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Ouellette
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Royall
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Stoecklin
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Quanxin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: sanesj@mcb.harvard.edu.
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Sanes
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98109, USA. Electronic address: julieha@alleninstitute.org.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Harris
mesh_terms: D000818:Animals; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D012160:Retina / Q000378:metabolism / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000502:physiology*; D013477:Superior Colliculi / Q000378:metabolism / Q000502:physiology; D014795:Visual Pathways / Q000502:physiology*
chemical_list: C045073:Cre recombinase; D019426:Integrases
keywords: Cre driver; axon projections; connectivity mapping; retinal ganglion cell types