title: Fluorescence Polarization Immunoassay Based on a New Monoclonal Antibody for the Detection of the Zearalenone Class of Mycotoxins in Maize.
abstract: To develop a sensitive fluorescence polarization immunoassay (FPIA) for screening the zearalenone class of mycotoxins in maize, two new monoclonal antibodies with uniform affinity to the zearalenone class and four fluorescein-labeled tracers were prepared. After careful selection of appropriate tracer-antibody pairs in terms of sensitivity and specificity, a FPIA that could simultaneously detect the zearalenone class with similar sensitivity was developed. Under optimum conditions, the half maximal inhibitory concentrations of the FPIA in buffer were 1.89, 1.97, 2.43, 1.99, 2.27, and 2.44 μg/L for zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol, and zearalanone, respectively. The limit of detection of FPIA for the zearalenone class was around 12 μg/kg in maize, and the recoveries ranged from 84.6 to 113.8%, with coefficients of variation below 15.3% in spiked samples. Finally, the FPIA was applied for screening naturally contaminated maize samples, and the results indicated a good correlation with that of high-performance liquid chromatography-tandem mass spectrometry.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.|Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , Beijing 100193, People's Republic of China.|Beijing Laboratory for Food Quality and Safety , Beijing 100193, People's Republic of China.
authors.forename: Xiya
authors.identifier: http://orcid.org/0000-0002-0996-9710
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University , Moscow 119991, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.|Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , Beijing 100193, People's Republic of China.|Beijing Laboratory for Food Quality and Safety , Beijing 100193, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wen
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.|Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , Beijing 100193, People's Republic of China.|Beijing Laboratory for Food Quality and Safety , Beijing 100193, People's Republic of China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.|Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , Beijing 100193, People's Republic of China.|Beijing Laboratory for Food Quality and Safety , Beijing 100193, People's Republic of China.
authors.forename: Chenglong
authors.identifier: http://orcid.org/0000-0003-3043-7230
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Genetic Toxicology, Shenzhen Center for Disease Control and Prevention , Shenzhen, Guangdong 518020, People's Republic of China.
authors.forename: Yuebin
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.|Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , Beijing 100193, People's Republic of China.|Beijing Laboratory for Food Quality and Safety , Beijing 100193, People's Republic of China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.|Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , Beijing 100193, People's Republic of China.|Beijing Laboratory for Food Quality and Safety , Beijing 100193, People's Republic of China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , Beijing 100193, People's Republic of China.|Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , Beijing 100193, People's Republic of China.|Beijing Laboratory for Food Quality and Safety , Beijing 100193, People's Republic of China.
authors.forename: Zhanhui
authors.identifier: http://orcid.org/0000-0002-0167-9559
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D005260:Female; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*; D015025:Zearalenone / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D009183:Mycotoxins; D015025:Zearalenone
keywords: detection; fluorescence polarization immunoassay; maize; monoclonal antibody; zearalenone