title: Cardiac Progenitor Cell Extraction from Human Auricles.
abstract: For many years, myocardial tissue has been considered terminally differentiated and, thus, incapable of regenerating. Recent studies have shown, instead, that cardiomyocytes, at least in part, are slowly substituted by new cells originating by precursor cells mostly embedded into the heart apex and in the atria. We have shown that an elective region of progenitor cell embedding is represented by the auricles, non-contractile atria appendages that can be easily sampled without harming the patient. The protocol here reported describes how from auricles a population of multipotent, cardiogenic cells can be isolated, cultured, and differentiated. Further studies are needed to fully exploit this cell population, but, sampling auricles, it could be possible to treat cardiac patients using their own cells circumventing rejection or organ shortage limitations.
authors.affiliation: Center for Regenerative Medicine, Università di Roma Tor Vergata, Via Orazio Raimondo, 00173, Rome, Italy. dinardo@uniroma2.it.|Department of Clinical Sciences and Translational Medicine, Università di Roma Tor Vergata, Via Orazio Raimondo, 00173, Rome, Italy. dinardo@uniroma2.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Nardo
authors.affiliation: Center for Regenerative Medicine, Università di Roma Tor Vergata, Via Orazio Raimondo, 00173, Rome, Italy.|Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Francesca
authors.initials: F
authors.lastname: Pagliari
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D006325:Heart Atria / Q000166:cytology*; D018189:Immunomagnetic Separation / Q000379:methods; D016130:Immunophenotyping; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D000950:Antigens, Ly; D015415:Biomarkers; C074650:Ly6a protein, mouse; D008565:Membrane Proteins
keywords: Cardiac progenitor cells; Differentiation; Heart; Immunomagnetic separation; SCA-1