title: A novelGossypium barbadenseERF transcription factor,GbERFb, regulation host response and resistance toVerticillium dahliaein tobacco.
abstract: Ethylene-responsive factors (ERFs) are commonly considered to play an important role in pathogen defense responses. However, only few of ERF members have been characterized in Sea island cotton (Gossypium barbadense). Here, we reported a novel AP2/ERF transcription factors gene, namedGbERFbwhich was cloned and identified from Sea island cotton by RACE. The expression ofGbERFbwas significantly induced by treatments with ethylene, Methyl jasmonate, salicylic acid, wounding, H2O2andVerticillium dahliae(V. dahliae) infection. Bioinformatics analysis showed that GbERFb protein containing a conserved ERF DNA binding domain and a nuclear localization signal sequence, belonged to IXb subgroup of the ERF family. Further experiments demonstrated that GbERFb could bind the GCC box cis-acting element and interact with GbMAPKb (MAP kinase) directly in yeast. Over-expression ofGbERFbin tobacco could increase the disease resistance toV. dahliae. The results suggest that theGbERFb, a new AP2/ERF transcription factor, could enhance the resistance toV. dahliaeand be useful in improvement of crop resistance to pathogenes.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, No. 598 HePing West Road, Shi Jiazhuang, 050000 Hebei Province China.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, No. 598 HePing West Road, Shi Jiazhuang, 050000 Hebei Province China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, No. 598 HePing West Road, Shi Jiazhuang, 050000 Hebei Province China.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, No. 598 HePing West Road, Shi Jiazhuang, 050000 Hebei Province China.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, No. 598 HePing West Road, Shi Jiazhuang, 050000 Hebei Province China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Du
authors.affiliation: School of Bioscience and Bioengineering, Hebei University of Science and Technology, Shi Jiazhuang, 050000 Hebei Province China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, No. 598 HePing West Road, Shi Jiazhuang, 050000 Hebei Province China.
authors.forename: Hanshuang
authors.initials: H
authors.lastname: Zhang
keywords: Disease resistance; ERF transcription factors; Gossypium barbadense; Verticillium dahliae