title: Identification and characterization of microsatellite markers inPinus kesiyavar.langbianensis(Pinaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed inPinus kesiyavar.langbianensis(Pinaceae), a species native to southwestern China, to investigate its genetic diversity and population structure in order to provide information for the conservation and management of this species.
  
  
  RESULTS
  Using next-generation sequencing, a total of 2349 putative simple sequence repeat primer pairs were designed. Eighteen polymorphic markers in 60 individuals belonging to four populations ofP. kesiyavar.langbianensiswere identified and characterized with two to 11 alleles per locus. The observed and expected heterozygosity ranged from 0.000 to 0.800 and 0.000 to 0.840, respectively. Each of these loci cross-amplified in the closely related speciesP. massoniana,P. densata,P. tabuliformis, andP. yunnanensis, with one to seven alleles per locus.
  
  
  CONCLUSIONS
  The new markers are promising tools to study the population genetics ofP. kesiyavar.langbianensisand related species.
authors.affiliation: Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China; Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, College of Forestry, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.
authors.forename: Nian-Hui
authors.initials: NH
authors.lastname: Cai
authors.affiliation: Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China; Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, College of Forestry, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Xu
authors.affiliation: Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Wang
authors.affiliation: Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, College of Forestry, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, College of Forestry, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.
authors.forename: Gen-Qian
authors.initials: GQ
authors.lastname: Li
keywords: Pinaceae; Pinus kesiya var. langbianensis; microsatellite; next-generation sequencing; population genetics