title: Conformational and thermal characterization of left ventricle remodeling post-myocardial infarction.
abstract: Adverse cardiac remodeling after myocardial infarction (MI) causes impaired ventricular function and heart failure. Histopathological characterization is commonly used to detect the location, size and shape of MI sites. However, the information about chemical composition, physical structure and molecular mobility of peri- and infarct zones post-MI is rather limited. The main objective of this work was to explore the spatiotemporal biochemical and biophysical alterations of key cardiac components post-MI. The FTIR spectra of healthy and remote myocardial tissue shows amides A, I, II and III associated with proteins in freeze-died tissue as major absorptions bands. In infarcted myocardium, the spectrum of these main absorptions was deeply altered. FITR evidenced an increase of the amide A band and the distinct feature of the collagen specific absorption band at 1338cm-1in the infarct area at 21days post-MI. At 21days post-MI, it also appears an important shift of amide I from 1646cm-1to 1637cm-1that suggests the predominance of the triple helical conformation in the proteins. The new spectra bands also indicate an increase in proteoglycans, residues of carbohydrates in proteins and polysaccharides in ischemic areas. Thermal analysis indicates a deep increase of unfreezable water/freezable water in peri- and infarcted tissues. In infarcted tissue is evidenced the impairment of myofibrillar proteins thermal profile and the emergence of a new structure. In conclusion, our results indicate a profound evolution of protein secondary structures in association with collagen deposition and reorganization of water involved in the scar maturation of peri- and infarct zones post-MI.
authors.affiliation: Physique des Polymères, Institut Carnot, CIRIMAT UMR 5085, Université Paul Sabatier, Bat 3R1B2, 118 route de Narbonne, 31062 Toulouse Cedex 04, France. Electronic address: valerie.samouillan@univ-tlse3.fr.
authors.forename: V
authors.initials: V
authors.lastname: Samouillan
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain; ICREC (Heart Failure and Cardiac Regeneration) Research Program, Health Sciences Research Institute Germans Trias i Pujol, 08916 Badalona, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Revuelta-López
authors.affiliation: ICREC (Heart Failure and Cardiac Regeneration) Research Program, Health Sciences Research Institute Germans Trias i Pujol, 08916 Badalona, Barcelona, Spain; CIBERCV, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Soler-Botija
authors.affiliation: Physique des Polymères, Institut Carnot, CIRIMAT UMR 5085, Université Paul Sabatier, Bat 3R1B2, 118 route de Narbonne, 31062 Toulouse Cedex 04, France.
authors.forename: J
authors.initials: J
authors.lastname: Dandurand
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.
authors.forename: Aleyda
authors.initials: A
authors.lastname: Benitez-Amaro
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Nasarre
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain; CIBERCV, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: D
authors.initials: D
authors.lastname: de Gonzalo-Calvo
authors.affiliation: ICREC (Heart Failure and Cardiac Regeneration) Research Program, Health Sciences Research Institute Germans Trias i Pujol, 08916 Badalona, Barcelona, Spain; CIBERCV, Instituto de Salud Carlos III, Madrid, Spain; Cardiology Service, Germans Trias i Pujol University Hospital, Badalona, Spain; Department of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Bayes-Genis
authors.affiliation: Physique des Polymères, Institut Carnot, CIRIMAT UMR 5085, Université Paul Sabatier, Bat 3R1B2, 118 route de Narbonne, 31062 Toulouse Cedex 04, France.
authors.forename: C
authors.initials: C
authors.lastname: Lacabanne
authors.affiliation: Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain; CIBERCV, Instituto de Salud Carlos III, Madrid, Spain; Institute of Biomedical Research of Barcelona (IIBB) - Spanish National Research Council (CSIC), Barcelona, Spain. Electronic address: cllorente@csic-iccc.org.
authors.forename: V
authors.initials: V
authors.lastname: Llorente-Cortés
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D020257:Ventricular Remodeling
chemical_list: D009124:Muscle Proteins
keywords: DSC; FITR; Myocardial infarction; Physical characterization