title: Changes in apolipoprotein B and oxidized low-density lipoprotein levels in gingival crevicular fluids as a result of periodontal tissue conditions.
abstract: OBJECTIVE
  Periodontal disease is a chronic inflammatory disease caused by bacterial infection that can lead to tooth loss. Gingival crevicular fluid can be collected easily and noninvasively. We previously discovered the presence of apolipoprotein B (apoB), the main constituent of low-density lipoprotein, and oxidized low-density lipoprotein (oxLDL) in the gingival crevicular fluid of healthy subjects. In this study, we investigated whether periodontal conditions affect the levels of apoB and oxLDL in gingival crevicular fluid.
  
  
  METHODS
  The study population comprised 11 patients with chronic periodontitis. A pair of gingival crevicular fluid samples was collected from each patient at a healthy site and at a site with periodontitis (baseline samples). Thereafter, gingival crevicular fluid samples were collected from the same patients again at 4 and 8 wk after scaling and root planing (SRP). The levels of apoB, oxLDL, protein and cytokines in gingival crevicular fluid, in addition to gingival crevicular fluid volume, were measured.
  
  
  RESULTS
  At baseline, the levels of apoB and oxLDL in gingival crevicular fluid were higher at the sites with periodontitis than at the healthy sites. The levels of apoB and oxLDL at periodontal sites decreased after SRP. The level of oxLDL in gingival crevicular fluid correlated well with the probing pocket depth. The oxLDL : apoB ratio in gingival crevicular fluid was significantly higher than that in plasma.
  
  
  CONCLUSIONS
  The levels of apoB and oxLDL in gingival crevicular fluid change according to the periodontal tissue conditions.
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Tokyo, Japan.|Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishizuka
authors.affiliation: Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kato
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Tokyo, Japan.|Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Moriya
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Noguchi
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Koide
authors.affiliation: Division of General Medicine, Department of Perioperative Medicine, Showa University School of Dentistry, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Inoue
authors.affiliation: Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Itabe
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000368:Aged; D001055:Apolipoproteins B / Q000032:analysis*; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000628:therapy; D016207:Cytokines / Q000032:analysis; D003774:Dental Plaque Index; D012534:Dental Scaling; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008077:Lipoproteins, LDL / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D016745:Root Planing
chemical_list: D001055:Apolipoproteins B; D016207:Cytokines; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein
keywords: apolipoprotein B; gingival crevicular fluid; initial preparation; oxidized low-density lipoprotein