title: Prdx1-encoded peroxiredoxin is important for vascular development in zebrafish.
abstract: Genetic signaling and redox homeostasis are required for proper growth of blood vessels. Here, we report a novel function of peroxiredoxin1 (Prdx1) in vascular development in zebrafish. Knockdown of prdx1 impairs the growth of intersegmental vessel and caudal vein plexus (CVP), and reduces the expression of vascular markers, thus suggesting a role for prdx1 in vasculature and indicating that the antioxidant function of prdx1 is important. We found that H2O2-treated embryos also have CVP defects and observed synergistic effects when prdx1 knockdown was combined with H2O2treatment. Moreover, N-acetyl-cysteine treatment rescues the vascular defects in prdx1 morphants. These results suggest that oxidative stress disturbs vascularization. Furthermore, we show that the regulation of prdx1 is mediated by Notch and BMP signals.
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors.forename: Po-Chun
authors.initials: PC
authors.lastname: Huang
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.|Department of Biotechnology, Kaohsiung Medical University, Taiwan.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.affiliation: Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan.|Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Taiwan.
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors.forename: Yi-Shan
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Syue
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Song
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Weng
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.|Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Tai
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.|Department of Biotechnology, Kaohsiung Medical University, Taiwan.|Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung, Taiwan.
authors.forename: Chang-Yi
authors.initials: CY
authors.lastname: Wu
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D001808:Blood Vessels / Q000196:embryology / Q000378:metabolism*; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D016877:Oxidants / Q000494:pharmacology; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D019485:Bone Morphogenetic Proteins; D016166:Free Radical Scavengers; D016877:Oxidants; D051880:Receptors, Notch; D029961:Zebrafish Proteins; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D000111:Acetylcysteine
keywords: peroxiredoxin1; vascular development; zebrafish