title: Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells.
abstract: BACKGROUND
  Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer.
  
  
  METHODS
  We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30μg/mL NPe6 for 24h. The cells were then irradiated with 0, 5, 15, or 30mW/cm2of laser power, and 0, 1, 5, 10, or 20J/cm2of laser energy. Cell viability was evaluated after 24h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10Hz, 50% duty) on Meth A cells.
  
  
  RESULTS
  The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15mW/cm2and a light dose of 20J/cm2were 18.6 and 5.0μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability.
  
  
  CONCLUSIONS
  NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan. Electronic address: tosaki@muses.tottori-u.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Institute for Medical Regulatory Science, Organization for University Research Initiatives, Waseda University, 513 Tsurumaki-cho, Waseda, Shinjuku-ku, Tokyo 162-0041, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kawase
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Institute for Medical Regulatory Science, Organization for University Research Initiatives, Waseda University, 513 Tsurumaki-cho, Waseda, Shinjuku-ku, Tokyo 162-0041, Japan; Faculty of Science and Engineering, Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iseki
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kishimoto
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Soko
authors.initials: S
authors.lastname: Ikuta
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Institute for Medical Regulatory Science, Organization for University Research Initiatives, Waseda University, 513 Tsurumaki-cho, Waseda, Shinjuku-ku, Tokyo 162-0041, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muragaki
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Yamashita
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Murahata
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Itoh
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: Joint Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D051379:Mice; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D011166:Porphyrins / Q000008:administration & dosage*; D011829:Radiation Dosage; D012509:Sarcoma / Q000188:drug therapy* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; C053434:Talaporfin
keywords: Continuous wave laser; Photodynamic therapy; Sarcoma; Squamous cell carcinoma; Square-wave modulated laser; Talaporfin sodium