title: Efficient automated synthesis of 2-(5-[18F]fluoropentyl)-2-methylmalonic acid ([18F]ML-10) on a commercial available [18F]FDG synthesis module.
abstract: [18F]ML-10 (2-(5-[18F]fluoro-pentyl)-2-methylmalonic acid) is a small molecule positron emission tomography (PET) probe for apoptosis imaging. Automated synthesis of [18F]ML-10 was developed by using two different purification methods through a direct saponification procedure on a modified commercial [18F]Fluoro-2-Deoxyglucose ([18F]FDG) synthesizer. C18 purification method 1: The final [18F]ML-10 solution containing ethanol was obtained with radiochemical yields of 60±5% (n=5) at the end of bombardment (EOB) and radiochemical purity of 98% in 35min. Al2O3and SCX purification method 2: To avoid possible side effects of a conventional ethanol-containing formulation, an new ethanol-free solution of [18F]ML-10 was also developed, the radiochemical yields was 50±5% (n=5, EOB) within 45min and the radiochemical purity was 98%.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China; Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals, Department of Nuclear Medicine, The First Affiliated Hospital, SunYat-sen University, Guangzhou 510080, China.
authors.forename: Shaoyu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals, Department of Nuclear Medicine, The First Affiliated Hospital, SunYat-sen University, Guangzhou 510080, China.
authors.forename: Dahong
authors.initials: D
authors.lastname: Nie
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. Electronic address: sjiang@tju.edu.cn.
authors.forename: Shende
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals, Department of Nuclear Medicine, The First Affiliated Hospital, SunYat-sen University, Guangzhou 510080, China. Electronic address: gtang0224@126.com.
authors.forename: Ganghua
authors.initials: G
authors.lastname: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis; D060326:Chemistry Techniques, Synthetic / Q000295:instrumentation; D004867:Equipment Design; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D019788:Fluorodeoxyglucose F18 / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D008764:Methylmalonic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000737:chemistry; D013679:Technology, Radiologic / Q000295:instrumentation
chemical_list: C532430:5-fluoropentyl-2-methylmalonic acid; D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D008764:Methylmalonic Acid
keywords: Automated synthesis; Ethanol-free formulation; [(18)F]FDG synthesis module; [(18)F]ML-10