title: Response of AtNPR1-expressing cotton plants toFusarium oxysporumf. sp.vasinfectumisolates.
abstract: In our earlier investigation, we had demonstrated that transgenic cotton plants expressing AtNPR1showed significant tolerance toFusarium oxysporumf. sp.vasinfectum,isolate 11 (Fov11) and several other pathogens. The current study was designed to further characterize the nature of the protection provided byAtNPR1expression and its limitations. Green Fluorescent Protein-expressingFov11was generated and used to study the progression of the disease within the plant. The results show that the spread of the pathogen was slower in the AtNPR1-transformants compared to the wild type plants. Transcript analysis in the seedling root and hypocotyl showed that the transgenic lines are capable of launching a stronger defense response when infected withFov11. We further confirmed that AtNPR1transformants showed greater degree of tolerance toFov11. However, little or no protection was observed against a related, but more virulent isolate,Fov43, and a highly virulent isolate, CA9.
authors.affiliation: Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843-2123 USA.
authors.forename: Sameer G
authors.initials: SG
authors.lastname: Joshi
authors.affiliation: Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843-2123 USA.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843-2123 USA.
authors.forename: Madhusudhana R
authors.initials: MR
authors.lastname: Janga
authors.affiliation: USDA-ARS, Southern Plains Agricultural Research Center, College Station, TX 77845 USA.
authors.forename: Alois A
authors.initials: AA
authors.lastname: Bell
authors.affiliation: Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843-2123 USA.|Deptartment of Soil & Crop Sciences, Texas A&M University, College Station, TX 77843-2474 USA.
authors.forename: Keerti S
authors.initials: KS
authors.lastname: Rathore
keywords: Cotton; Disease resistance; Fusarium wilt; NPR1; Transgenic cotton