title: Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage.
abstract: OBJECTIVE
  Squamous cell carcinoma or SCC of horn in bovines (bovine horn core carcinoma) frequently observed inBos indicusaffecting almost 1% of cattle population. Freshly isolated primary epithelial cells may be closely related to the malignant epithelial cells of the tumor. Comparison of gene expression in between horn's SCC tissue and its early passage primary culture using next generation sequencing was the aim of this study.
  
  
  METHODS
  Whole transcriptome sequencing of horn's SCC tissue and its early passage cells using Ion Torrent PGM were done. Comparative expression and analysis of different genes and pathways related to cancer and biological processes associated with malignancy, proliferating capacity, differentiation, apoptosis, senescence, adhesion, cohesion, migration, invasion, angiogenesis, and metabolic pathways were identified.
  
  
  RESULTS
  Up-regulated genes in SCC of horn's early passage cells were involved in transporter activity, catalytic activity, nucleic acid binding transcription factor activity, biogenesis, cellular processes, biological regulation and localization and the down-regulated genes mainly were involved in focal adhesion, extracellular matrix receptor interaction and spliceosome activity.
  
  
  CONCLUSIONS
  The experiment revealed similar transcriptomic nature of horn's SCC tissue and its early passage cells.
authors.affiliation: Veterinary Officer (WBAH & VS), West Bengal Animal Resources Development Department, Bankura - 772 152, West Bengal, India; Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: Sharadindu
authors.initials: S
authors.lastname: Shil
authors.affiliation: Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Joshi
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: C G
authors.initials: CG
authors.lastname: Joshi
authors.affiliation: Hester Biosciences Limited, Ahmedabad, Gujarat, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Patel
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Shah
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: Namrata
authors.initials: N
authors.lastname: Patel
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: Subhash J
authors.initials: SJ
authors.lastname: Jakhesara
authors.affiliation: Veterinary Officer, MVC Sarenga, Government of West Bengal, Bankura, West Bengal, India.
authors.forename: Sumana
authors.initials: S
authors.lastname: Kundu
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Reddy
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: P G
authors.initials: PG
authors.lastname: Koringa
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India; Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
authors.forename: D N
authors.initials: DN
authors.lastname: Rank
keywords: RNA-sequencing; cummerbund; gene ontology; primary culture; squamous cell carcinoma of horn; transcriptome profiling