title: Elucidation of the Role of Dorsal Raphe Serotonergic Neurons in Mood Regulation Using Pharmacological and Viral Vector-based Approaches.
abstract: Growing evidence indicates that serotonergic neurons play a crucial role in brain function and dysfunction, such as in major depressive disorder. However, the complexity of serotonergic projections severely hampers the elucidation of their precise mechanisms. Here we summarize our recent studies on the effects of ketamine and olanzapine, which have been reported to be effective in treatment-resistant depression, on dorsal raphe nucleus serotonergic neurons, using microdialysis, electrophysiology experiments, and slice cultures. Furthermore, we discuss the results of our recent approach using viral vectors, in particular, HIV-1 based lentiviral vectors.
authors.affiliation: iPS Cell-based Research Project on Brain Neuropharmacology and Toxicology, Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nagayasu
mesh_terms: D000818:Animals; D001569:Benzodiazepines / Q000494:pharmacology / Q000627:therapeutic use; D001921:Brain / Q000502:physiology / Q000503:physiopathology; D003866:Depressive Disorder / Q000188:drug therapy* / Q000209:etiology* / Q000503:physiopathology; D065847:Dorsal Raphe Nucleus / Q000166:cytology*; D004594:Electrophysiology; D005822:Genetic Vectors; D015497:HIV-1; D006801:Humans; D066298:In Vitro Techniques; D007649:Ketamine / Q000494:pharmacology / Q000627:therapeutic use; D016086:Lentivirus; D051379:Mice; D017551:Microdialysis; D000077152:Olanzapine; D051381:Rats; D059326:Serotonergic Neurons / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism
chemical_list: D001569:Benzodiazepines; D012701:Serotonin; D007649:Ketamine; D000077152:Olanzapine