title: Phosphorylation of histone H3 on Ser-10 by Aurora B is essential for chromosome condensation in porcine embryos during the first mitotic division.
abstract: Phosphorylation of histone H3 on Ser-10 (H3S10ph) is involved in regulating mitotic chromosome condensation and decondensation, which plays an important regulatory role during mitotic cell cycle progression in mammalian cells. However, whether H3S10ph plays a similar role in early porcine embryos during the first mitotic division remains uncertain. In this study, the subcellular localization and possible roles of H3S10ph were evaluated in the first mitotic cell cycle progression of porcine embryos using western blot, indirect immunofluorescence and barasertib (H3S10ph upstream regulator Aurora-B inhibitor) treatments. H3S10ph exhibited a dynamic localization pattern and was localized to chromosomes from prometaphase to anaphase stages. Treatment of porcine embryos with barasertib inhibited mitotic division at the prophase stage and was associated with a defect in chromosome condensation accompanied by the reduction of H3S10ph. These results indicated that H3S10ph is involved in the first mitotic division in porcine embryos through its regulatory function in chromosome condensation, which further affects porcine embryo cell cycle progression during mitotic division.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China.
authors.forename: Changchao
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China.
authors.forename: Zixiao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China.
authors.forename: Yaya
authors.initials: Y
authors.lastname: Liao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Yao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Rui
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China. jusq@njau.edu.cn.
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Ju
mesh_terms: D000818:Animals; D064107:Aurora Kinase B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020090:Chromosome Segregation / Q000187:drug effects; D033481:Chromosomes, Mammalian / Q000378:metabolism*; D006657:Histones / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D008938:Mitosis / Q000187:drug effects; D010755:Organophosphates / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D010768:Phosphoserine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011799:Quinazolines / Q000494:pharmacology; D013552:Swine / Q000196:embryology* / Q000235:genetics*
chemical_list: C520647:2-((3-((4-((5-(2-((3-fluorophenyl)amino)-2-oxoethyl)-1H-pyrazol-3-yl)amino)quinazolin-7-yl)oxy)propyl)(ethyl)amino)ethyl dihydrogen phosphate; D006657:Histones; D010755:Organophosphates; D011799:Quinazolines; D010768:Phosphoserine; D064107:Aurora Kinase B
keywords: Chromosome condensation; Embryos; H3S10ph; Mitotic division; Pig