title: Rationale and Design of a Genetic Study on Cardiometabolic Risk Factors: Protocol for the Tehran Cardiometabolic Genetic Study (TCGS).
abstract: BACKGROUND
  Cardiometabolic risk factors comprise cardiovascular diseases and/or diabetes, and need to be evaluated in different fields.
  
  
  OBJECTIVE
  The primary aim of the Tehran Cardiometabolic Genetic Study (TCGS) is to create a comprehensive genome-wide database of at least 16,000 Tehranians, who are participants of the ongoing Tehran Lipid and Glucose Study (TLGS) cohort.
  
  
  METHODS
  TCGS was designed in collaboration with the Research Institute for Endocrine Sciences and the genetic company deCODE. Participants had already been followed for over a 20-year period for major cardiometabolic-related health events including myocardial infarction, stroke, diabetes mellitus, hypertension, obesity, hyperlipidemia, and familial hypercholesterolemia.
  
  
  RESULTS
  The TCGS cohort described here comprises 17,186 (86.3%) of the 19,905 TLGS participants who provided a baseline blood sample that was adequate for plasma and deoxyribonucleic acid analysis. This study is comprised of 849 individuals and 3109 families with at least one member having genotype information. Finally, 5977 males and 7422 females with the total genotyping rate of 0.9854 were genotyped with HumanOmniExpress-24-v1-0 bead chips (containing 649,932 single-nucleotide polymorphism loci with an average mean distance of 4 kilobases).
  
  
  CONCLUSIONS
  Investigations conducted within the TCGS will seek to identify relevant patterns of genetic polymorphisms that could be related to cardiometabolic risk factors in participants from Tehran. By linking genome-wide data to the existing databank of TLGS participants, which includes comprehensive behavioral, biochemical, and clinical data on each participant since cohort inception in 1999, the TCGS will also allow exploration of gene-gene and gene-environment interactions as they relate to disease status.
authors.affiliation: Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Maryam S
authors.identifier: http://orcid.org/0000-0002-2208-6568
authors.initials: MS
authors.lastname: Daneshpour
authors.affiliation: Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Mohammad-Sadegh
authors.identifier: http://orcid.org/0000-0002-0391-6538
authors.initials: MS
authors.lastname: Fallah
authors.affiliation: Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Bahareh
authors.identifier: http://orcid.org/0000-0002-7665-8648
authors.initials: B
authors.lastname: Sedaghati-Khayat
authors.affiliation: Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Kamran
authors.identifier: http://orcid.org/0000-0002-8379-9668
authors.initials: K
authors.lastname: Guity
authors.affiliation: Prevention Of Metabolic Disorders Research Center, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Davood
authors.identifier: http://orcid.org/0000-0003-4956-1039
authors.initials: D
authors.lastname: Khalili
authors.affiliation: Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Mehdi
authors.identifier: http://orcid.org/0000-0001-6775-8148
authors.initials: M
authors.lastname: Hedayati
authors.affiliation: Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Ahmad
authors.identifier: http://orcid.org/0000-0002-5160-8978
authors.initials: A
authors.lastname: Ebrahimi
authors.affiliation: Prevention Of Metabolic Disorders Research Center, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Farhad
authors.identifier: http://orcid.org/0000-0001-6016-1867
authors.initials: F
authors.lastname: Hajsheikholeslami
authors.affiliation: Nutrition And Endocrine Research Center, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Parvin
authors.identifier: http://orcid.org/0000-0003-2391-4924
authors.initials: P
authors.lastname: Mirmiran
authors.affiliation: Reproductive Endocrinology Research Center, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Fahimeh
authors.identifier: http://orcid.org/0000-0002-4609-065X
authors.initials: F
authors.lastname: Ramezani Tehrani
authors.affiliation: Prevention Of Metabolic Disorders Research Center, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Amir-Abbas
authors.identifier: http://orcid.org/0000-0001-7339-0693
authors.initials: AA
authors.lastname: Momenan
authors.affiliation: Prevention Of Metabolic Disorders Research Center, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Arash
authors.identifier: http://orcid.org/0000-0001-9662-2944
authors.initials: A
authors.lastname: Ghanbarian
authors.affiliation: Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Atieh
authors.identifier: http://orcid.org/0000-0003-1046-0003
authors.initials: A
authors.lastname: Amouzegar
authors.affiliation: Research Center For Social Determinants Of Endocrine Health, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Parisa
authors.identifier: http://orcid.org/0000-0002-3198-4941
authors.initials: P
authors.lastname: Amiri
authors.affiliation: Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
authors.forename: Fereidoun
authors.identifier: http://orcid.org/0000-0002-7335-3873
authors.initials: F
authors.lastname: Azizi
keywords: cardiometabolic disorders; cardiovascular; genetic; genome-wide association study