title: Quisinostat treatment improves histone acetylation and developmental competence of porcine somatic cell nuclear transfer embryos.
abstract: Abnormal epigenetic modifications are considered a main contributing factor to low cloning efficiency. In the present study, we explored the effects of quisinostat, a novel histone deacetylase inhibitor, on blastocyst formation rate in porcine somatic-cell nuclear transfer (SCNT) embryos, on acetylation of histone H3 lysine 9 (AcH3K9), and on expression of POU5F1 protein and apoptosis-related genes BAX and BCL2. Our results showed that treatment with 10 nM quisinostat for 24 hr significantly improved the development of reconstructed embryos compared to the untreated group (19.0 ± 1.6% vs. 10.2 ± 0.9%; p < 0.05). Quisinostat-treated SCNT embryos also possessed significantly increased AcH3K9 at the pseudo-pronuclear stage (p < 0.05), as well as improved immunostaining intensity for POU5F1 at the blastocyst stage (p < 0.05). While no statistical difference in BAX expression was observed, BCL2 transcript abundance was significantly different in the quisinostat-treated compared to the untreated control group. Of the 457 quisinostat-treated cloned embryos transferred into three surrogates, six fetuses developed from the one sow that became pregnant. These findings suggested that quisinostat can regulate gene expression and epigenetic modification, facilitating nuclear reprogramming, and subsequently improving the developmental competence of pig SCNT embryos and blastocyst quality.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhu
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Xing
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Guang-Lei
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Mei-Fu
authors.initials: MF
authors.lastname: Xuan
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Qi-Rong
authors.initials: QR
authors.lastname: Luo
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Zhao-Bo
authors.initials: ZB
authors.lastname: Luo
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Xi-Jun
authors.identifier: 0000-0001-8544-6236
authors.initials: XJ
authors.lastname: Yin
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Jin-Dan
authors.initials: JD
authors.lastname: Kang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D019976:Cloning, Organism; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D044127:Epigenesis, Genetic / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D013552:Swine; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D006877:Hydroxamic Acids; D050814:Octamer Transcription Factor-3; D051028:bcl-2-Associated X Protein; C541788:quisinostat
keywords: histone acetylation; histone deacetylase inhibitors; porcine; quisinostat; somatic cell nuclear transfer