title: Gradual decrease in spermatogenesis caused by chronic stress.
abstract: Chronic stress induces decreased sperm motility, viability and concentration in stressed males. Also, stress modifies oxidative status and causes apoptosis in testes, as well as a decrease in the epithelial area of seminiferous tubules. However, there are no studies that analyze the alterations caused by stress in testicular cells. Thus, in this study, alterations in the morphology of testicular germ cells caused by different days of chronic stress were assessed. Adult male rats were exposed to stress by immersion in cold water (ICW) daily for 3, 8, 20 or 50 consecutive days. Plasma testosterone and corticosterone were also assessed. Results showed that chronic stress causes loss of germ cells, and alteration of spermatogenesis. Seminiferous tubules from stressed males showed several degenerative signs, such as vacuoles in the basal epithelium, with picnotic indicia; moderate to severe exfoliation of degenerative germinal cells in the tubule lumen was also observed. These alterations were observed in all days of stress in a gradual way, from day 3-50. Testosterone levels were decreased at all those times, and corticosterone concentrations were increased on the same days. These results show that chronic stress causes severe damage to germ cells, which can account for infertility problems in males. These alterations are related to a decrease in testosterone as well as an increase in corticosterone caused by stress.
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana Iztapalapa, San Rafael Atlixco 186, C.P. 09340, Mexico City, Mexico.
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Juárez-Rojas
authors.affiliation: Instituto Nacional de Pediatría, Calzada México Xochimilco No. 101, Colonia San Lorenzo Huipulco, Tlalpan, C.P. 14370, Mexico City, Mexico.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Vigueras-Villaseñor
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana Iztapalapa, San Rafael Atlixco 186, C.P. 09340, Mexico City, Mexico.
authors.forename: Fahiel
authors.initials: F
authors.lastname: Casillas
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana Iztapalapa, San Rafael Atlixco 186, C.P. 09340, Mexico City, Mexico. Electronic address: rems@xanum.uam.mx.
authors.forename: Socorro
authors.initials: S
authors.lastname: Retana-Márquez
mesh_terms: D000818:Animals; D001835:Body Weight; D003345:Corticosterone / Q000097:blood; D008297:Male; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000503:physiopathology; D013091:Spermatogenesis / Q000502:physiology*; D013312:Stress, Physiological; D013737:Testis / Q000166:cytology / Q000503:physiopathology*; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D003345:Corticosterone
keywords: Corticosterone; Germ cells; Seminiferous epithelium; Spermatogenesis; Stress; Testis; Testosterone