title: Methods for Long-Term Storage of Murine Bone Marrow-Derived Mesenchymal Stem Cells.
abstract: This chapter is based on a simplified method to validate the current preservation procedure of mesenchymal stem cells (MSCs). Currently, there are various media available for freezing and thus preserving the MSCs, making it hard to decide which agent will be apt for cellular requirements. The study describes the effect of two different compositions of freezing media used in regular cell culture experiments, on the morphology, proliferation, and doubling rate of MSCs. Commonly used agents for the cryopreservation of MSCs include DMSO (Dimethyl Sulfoxide) and FBS (Fetal Bovine Serum) and DMEM (Dulbecco's Modified Eagle Medium). To ascertain that the currently used agents do not lead to major changes in the MSC morphology and proliferation, the cells are frozen using the above-mentioned agents in different groups and then their effects analyzed. Thus, the chapter helps to decide what reagents can suit the MSCs, hence minimizing the laboratory to laboratory variability of their characteristics.
authors.affiliation: Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Niketa
authors.initials: N
authors.lastname: Sareen
authors.affiliation: Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Ejlal
authors.initials: E
authors.lastname: Abu-El-Rub
authors.affiliation: Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Glen Lester
authors.initials: GL
authors.lastname: Sequiera
authors.affiliation: Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Meenal
authors.initials: M
authors.lastname: Moudgil
authors.affiliation: Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, R 3028-2, 351 Tache Ave., Winnipeg, MB, R2H2A6, Canada. sdhingra@sbrc.ca.
authors.forename: Sanjiv
authors.initials: S
authors.lastname: Dhingra
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D051381:Rats
chemical_list: D003451:Cryoprotective Agents
keywords: Cryopreservation; DMSO; FBS; Freezing media; Mesenchymal stem cells