title: Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone.
abstract: Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Kazemzadeh-Narbat
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Department of Biomechanical Engineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7522NB, Enschede, The Netherlands.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Rouwkema
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.|Department of Chemical Engineering, Northeastern University, Boston, MA, 02115-5000, USA.
authors.forename: Nasim
authors.initials: N
authors.lastname: Annabi
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Ghaderi
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Cha
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Mansi
authors.initials: M
authors.lastname: Aparnathi
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Akbar
authors.initials: A
authors.lastname: Khalilpour
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Batzaya
authors.initials: B
authors.lastname: Byambaa
authors.affiliation: Biomimetic Materials and Tissue Engineering Laboratories, Department of Chemical Engineering, University of South Carolina, Columbia, SC, 29208, USA.
authors.forename: Esmaiel
authors.initials: E
authors.lastname: Jabbari
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Tamayol
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.|Department of Physics, King Abdulaziz University, Jeddah, 21569, Saudi Arabia.|Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 05029, Republic of Korea.
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010012:Osteogenesis / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D020100:Hydrogels
keywords: bone tissue engineering; hydrogels; micropatterning; vascularization