title: Identification and molecular epidemiology of dermatophyte isolates by repetitive-sequence-PCR-based DNA fingerprinting using the DiversiLab system in Turkey.
abstract: Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep-PCR. In the identification of dermatophyte isolates, agreement between rep-PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A -D) which were determined by the use of rep-PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep-PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep-PCR.
authors.affiliation: Department of Microbiology, Erciyes University Medical Faculty, Kayseri, Turkey.
authors.forename: A Nedret
authors.identifier: http://orcid.org/0000-0002-1736-9707
authors.initials: AN
authors.lastname: Koc
authors.affiliation: Department of Microbiology, Erciyes University Medical Faculty, Kayseri, Turkey.
authors.forename: Mustafa A
authors.initials: MA
authors.lastname: Atalay
authors.affiliation: Department of Medical Microbiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Melek
authors.initials: M
authors.lastname: Inci
authors.affiliation: Department of Microbiology, Kayseri Research and Training Hospital, Kayseri, Turkey.
authors.forename: Fatma M
authors.initials: FM
authors.lastname: Sariguzel
authors.affiliation: Department of Microbiology, Cerrahpaşa Medical Faculty, İstanbul, Turkey.
authors.forename: Hafize
authors.initials: H
authors.lastname: Sav
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003883:Arthrodermataceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D003881:Dermatomycoses / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D014249:Trichophyton / Q000145:classification / Q000235:genetics*; D014421:Turkey / Q000453:epidemiology; D055815:Young Adult
chemical_list: D004271:DNA, Fungal
keywords: DNA sequencing analysis; Trichophyton mentagrophytes; Trichophyton rubrum; dermatophyte isolates; genotyping; repetitive sequence-based PCR by DiversiLab system