title: Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, During Bacterial Infection in Arabidopsis.
abstract: The plant cell wall responds dynamically during interaction with various pathogens. Upon recognition of "nonself" components, plant cells deploy a variety of immune responses including cell wall fortification. Callose, a β-(1, 3)-D-glucan polymer, is a component of the material deposited at the site of infection between the plasma membrane and the preexisting cell wall that is hypothesized to serve as a physical barrier and platform for directed antimicrobial compound deposition. The defense-associated function of callose deposition is supported by its induction during pathogen-associated molecular patterns (PAMP)-triggered immunity (PTI) and its inhibition by defense suppressing virulence effectors. Thus, callose deposition is a commonly monitored read-out in plant defense. This protocol describes the use of aniline blue staining and fluorescent microscopy to measure callose deposition in bacteria-infected or elicitor-challenged Arabidopsis leaf tissues.
authors.affiliation: Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, 43210, USA. mackey.86@osu.edu.|Department of Molecular Genetics, The Ohio State University, Columbus, OH, 43210, USA. mackey.86@osu.edu.
authors.forename: David M
authors.initials: DM
authors.lastname: Mackey
mesh_terms: D017360:Arabidopsis / Q000276:immunology / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000032:analysis*; D008856:Microscopy, Fluorescence; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D044224:Pseudomonas syringae / Q000276:immunology / Q000472:pathogenicity*
chemical_list: D029681:Arabidopsis Proteins; D005936:Glucans; D000069452:Pathogen-Associated Molecular Pattern Molecules; C048306:callose
keywords: Aniline blue; Arabidopsis; Callose; Flg22; PTI; Pseudomonas syringae