title: Allergenome characterization of the mosquito Aedes aegypti.
abstract: BACKGROUND
  Saliva and muscle-derived mosquito allergens have been purified and characterized. However, the complete set of allergens remains to be elucidated. In this study, we identified and characterized IgE-binding proteins from the mosquito species Aedes aegypti.
  
  
  METHODS
  Serum was obtained from 15 allergic individuals with asthma and/or rhinitis and sensitized to mosquito. IgE binding was determined by ELISA. Total proteins from freeze-dried bodies of A. aegypti were extracted and IgE-reactive proteins were identified by 2D gel electrophoresis, followed by Western blot with pooled or individual sera. IgE-reactive spots were further characterized by mass spectrometry.
  
  
  RESULTS
  Twenty-five IgE-reactive spots were identified, corresponding to 10 different proteins, some of which appeared as different variants or isoforms. Heat-shock cognate 70 (HSC-70) and tropomyosin showed IgE reactivity with 60% of the sera, lysosomal aspartic protease, and "AAEL006070-PA" (Uniprot: Q177P3) with 40% and the other proteins with <33.3% of the sera. Different variants or isoforms of tropomyosin, arginine or creatine kinase, glyceraldehyde-3-phosphate dehydrogenase (GPDH), calcium-binding protein, and phosphoglycerate mutase were also identified. The mixture of three allergens (Aed a 6, Aed a 8, and Aed a 10) seems to identify more than 80% of A. aegypti-sensitized individuals, indicating that these allergens should be considered when designing of improved mosquito allergy diagnostic tools.
  
  
  CONCLUSIONS
  The newly identified allergens may play a role in the pathophysiology of mosquito allergy in the tropics, and some of them might be important arthropod-related proteins involved in cross-reactivity between A. aegypti and other allergenic arthropods.
authors.affiliation: Institute for Immunological Research/University of Cartagena, Cartagena, Colombia.
authors.forename: J F
authors.initials: JF
authors.lastname: Cantillo
authors.affiliation: Institute for Immunological Research/University of Cartagena, Cartagena, Colombia.
authors.forename: L
authors.initials: L
authors.lastname: Puerta
authors.affiliation: Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Puchalska
authors.affiliation: Cabinet de Inmunoallergology, Fort de France, Martinique, France.
authors.forename: S
authors.initials: S
authors.lastname: Lafosse-Marin
authors.affiliation: Inmunotek S.L., Alcalá de Henares, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Subiza
authors.affiliation: Inmunotek S.L., Alcalá de Henares, Spain.|Division of Allergy and Immunology, University of South Florida, Tampa, FL, USA.
authors.forename: E
authors.identifier: http://orcid.org/0000-0001-5873-6932
authors.initials: E
authors.lastname: Fernández-Caldas
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000276:immunology; D000485:Allergens / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D049750:Genome, Insect; D023281:Genomics / Q000379:methods; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D040901:Proteomics / Q000379:methods
chemical_list: D000485:Allergens; D060829:Arthropod Proteins; D007073:Immunoglobulin E
keywords: 
  Aedes aegypti
  ; allergen; allergenome; insect allergy; mosquito