title: Assays to Investigate the N-Glycosylation State and Function of Plant Pattern Recognition Receptors.
abstract: The biogenesis and functionality of pattern recognition receptors (PRRs) are critical for robust plant immune responses. Here, we present methods to determine the N-glycosylation state and ligand-induced activity of these receptors for comparative quantitative analysis. These techniques can be used to identify mutants and chemical inhibitors affecting PRR biogenesis and functionality. When combined, these techniques can provide useful insights on biological processes necessary to synthesize a properly membrane-localized and ligand-responsive PRR.
authors.affiliation: Department of Molecular, Cellular & Developmental Biology, Yale University, 266 Whitney Ave, New Haven, CT, 06510, USA.
authors.forename: Stacey A
authors.initials: SA
authors.lastname: Lawrence
authors.affiliation: Department of Molecular, Cellular & Developmental Biology, Yale University, 266 Whitney Ave, New Haven, CT, 06510, USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ceserani
authors.affiliation: Department of Molecular, Cellular & Developmental Biology, Yale University, 266 Whitney Ave, New Haven, CT, 06510, USA. nicole.clay@yale.edu.
authors.forename: Nicole K
authors.initials: NK
authors.lastname: Clay
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D006031:Glycosylation; D008024:Ligands; D009154:Mutation; D051192:Receptors, Pattern Recognition / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008024:Ligands; D051192:Receptors, Pattern Recognition
keywords: Anthocyanin inhibition; Asparagine-linked glycosylation; Endoglycosidase H; MAPK activation; Protein quality control; Seedling growth arrest; Tunicamycin