title: Whole-brain metallomic analysis of the common marmoset (Callithrix jacchus).
abstract: Despite the importance of transition metals for normal brain function, relatively little is known about the distribution of these elemental species across the different tissue compartments of the primate brain. In this study, we employed laser ablation-inductively coupled plasma-mass spectrometry on PFA-fixed brain sections obtained from two adult common marmosets. Concurrent cytoarchitectonic, myeloarchitectonic, and chemoarchitectonic measurements allowed for identification of the major neocortical, archaecortical, and subcortical divisions of the brain, and precise localisation of iron, manganese, and zinc concentrations within each division. Major findings across tissue compartments included: (1) differentiation of white matter tracts from grey matter based on manganese and zinc distribution; (2) high iron concentrations in the basal ganglia, cortex, and substantia nigra; (3) co-localization of high concentrations of iron and manganese in the primary sensory areas of the cerebral cortex; and (4) high manganese in the hippocampus. The marmoset has become a model species of choice for connectomic, aging, and transgenic studies in primates, and the application of metallomics to these disciplines has the potential to yield high translational and basic science value.
authors.affiliation: Department of Physiology, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Clayton, Victoria 3800, Australia and Research School, Ruhr University Bochum, Bochum, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Knauer
authors.affiliation: Laboratory of Neuroinformatics, Department of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Majka
authors.affiliation: Department of Physiology, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: K J
authors.initials: KJ
authors.lastname: Watkins
authors.affiliation: Department of Physiology, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: A W R
authors.initials: AWR
authors.lastname: Taylor
authors.affiliation: Department of Physiology, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Malamanova
authors.affiliation: School of Earth Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia and The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, 3010, Victoria, Australia.
authors.forename: B
authors.initials: B
authors.lastname: Paul
authors.affiliation: Department of Physiology, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Hsin-Hao
authors.initials: HH
authors.lastname: Yu
authors.affiliation: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, 3010, Victoria, Australia.
authors.forename: A I
authors.identifier: 0000-0001-8259-9069
authors.initials: AI
authors.lastname: Bush
authors.affiliation: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, 3010, Victoria, Australia and Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, NSW, Australia.
authors.forename: D J
authors.identifier: 0000-0002-5922-7643
authors.initials: DJ
authors.lastname: Hare
authors.affiliation: Department of Physiology, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Clayton, Victoria 3800, Australia and Graduate Entry Medical Program, School of Rural Health, Monash University, Churchill, Victoria 3842, Australia.
authors.forename: D H
authors.identifier: 0000-0002-5130-1255
authors.initials: DH
authors.lastname: Reser
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry; D002144:Callithrix / Q000033:anatomy & histology / Q000378:metabolism; D005260:Female; D006801:Humans; D007501:Iron / Q000032:analysis*; D008297:Male; D008345:Manganese / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D013045:Species Specificity; D015032:Zinc / Q000032:analysis*
chemical_list: D008345:Manganese; D007501:Iron; D015032:Zinc