title: The gene expression of the neuronal protein, SLC38A9, changes in mouse brain after in vivo starvation and high-fat diet.
abstract: SLC38A9 is characterized as a lysosomal component of the amino acid sensing Ragulator-RAG GTPase complex, controlling the mechanistic target of rapamycin complex 1 (mTORC1). Here, immunohistochemistry was used to map SLC38A9 in mouse brain and staining was detected throughout the brain, in cortex, hypothalamus, thalamus, hippocampus, brainstem and cerebellum. More specifically, immunostaining was found in areas known to be involved in amino acid sensing and signaling pathways e.g. piriform cortex and hypothalamus. SLC38A9 immunoreactivity co-localized with both GABAergic and glutamatergic neurons, but not with astrocytes. SLC38A9 play a key role in the mTORC1 pathway, and therefore we performed in vivo starvation and high-fat diet studies, to measure gene expression alterations in specific brain tissues and in larger brain regions. Following starvation, Slc38a9 was upregulated in brainstem and cortex, and in anterior parts of the brain (Bregma 3.2 to -2.1mm). After high-fat diet, Slc38a9 was specifically upregulated in hypothalamus, while overall downregulation was noticed throughout the brain (Bregma 3.2 to -8.6mm).
authors.affiliation: Department of Pharmaceutical Bioscience, Molecular Neuropharmacology, Uppsala University, Uppsala SE, Sweden.
authors.forename: Sofie V
authors.identifier: http://orcid.org/0000-0001-7367-0257
authors.initials: SV
authors.lastname: Hellsten
authors.affiliation: Department of Pharmaceutical Bioscience, Molecular Neuropharmacology, Uppsala University, Uppsala SE, Sweden.
authors.forename: Mikaela M
authors.initials: MM
authors.lastname: Eriksson
authors.affiliation: Department of Pharmaceutical Bioscience, Molecular Neuropharmacology, Uppsala University, Uppsala SE, Sweden.
authors.forename: Emilia
authors.initials: E
authors.lastname: Lekholm
authors.affiliation: Department of Pharmaceutical Bioscience, Molecular Neuropharmacology, Uppsala University, Uppsala SE, Sweden.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Arapi
authors.affiliation: Department of Pharmaceutical Bioscience, Molecular Neuropharmacology, Uppsala University, Uppsala SE, Sweden.
authors.forename: Emelie
authors.initials: E
authors.lastname: Perland
authors.affiliation: Department of Pharmaceutical Bioscience, Molecular Neuropharmacology, Uppsala University, Uppsala SE, Sweden.
authors.forename: Robert
authors.initials: R
authors.lastname: Fredriksson
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D059305:Diet, High-Fat; D015870:Gene Expression; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D013217:Starvation / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D026905:Amino Acid Transport Systems; C000621977:SLC38A9 protein, mouse