title: Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications.
abstract: Bone marrow endothelial progenitor cells (EPCs) have shown a great promise to promote ischemic tissue neovascularization and to attenuate ischemic injury in a variety of animal models, which led to EPC-based clinical trials that yielded modest but promising results. Some of the variables in the use of EPCs relate to their differential isolation and characterization protocols since the EPC literature does not identify a unique marker for these vascular progenitors. In this chapter, we present step-by-step protocols for the isolation of EPCs, their characterization and culture conditions, and their potential use in basic and clinical research.
authors.affiliation: Center for Translational Medicine, Lewis Katz School of Medicine, Temple University, MERB-953, 3500 N Broad Street, Philadelphia, PA, 19140, USA.
authors.forename: Venkata N S
authors.initials: VNS
authors.lastname: Garikipati
authors.affiliation: Center for Translational Medicine, Lewis Katz School of Medicine, Temple University, MERB-953, 3500 N Broad Street, Philadelphia, PA, 19140, USA. raj.kishore@temple.edu.|Department of Pharmacology, Temple University, Philadelphia, PA, 19140, USA. raj.kishore@temple.edu.
authors.forename: Raj
authors.initials: R
authors.lastname: Kishore
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D018919:Neovascularization, Physiologic; D012038:Regeneration; D012106:Research; D033581:Stem Cell Transplantation
chemical_list: D015415:Biomarkers
keywords: Angiogenesis; Bone marrow; Endothelial; Neo-vascularization; Progenitor cells