title: Novel serotype of bluetongue virus in South America and first report of epizootic haemorrhagic disease virus in Ecuador.
abstract: Bluetongue virus (BTV) and Epizootic haemorrhagic disease virus (EHDV) are closely related Orbiviruses that affect domestic and wild ruminants. In Ecuador previous serological studies reported the presence of BTV; however, no data are available about the presence of EHDV. In this study, 295 cattle without symptoms of infection were sampled from two farms located in Andean and Amazonian regions and from a slaughterhouse in the coastal region. ELISA analyses showed high prevalence of BTV (98.9%) and EHDV (81.3%) antibodies, and RT-qPCRs revealed the presence of EHDV (24.1%) and BTV (10.2%) genomes in cattle blood samples. Viral isolation allowed to identify EHDV serotype 1 (EHDV1) and BTV serotypes 9 (BTV9), 13 and 18. These findings suggest that BTV and EHDV are enzootic diseases in Ecuador.
authors.affiliation: Instituto de Microbiología, Universidad San Francisco de Quito, Quito, Ecuador.
authors.forename: J
authors.initials: J
authors.lastname: Verdezoto
authors.affiliation: UMR 1161 ANSES/INRA/ENVA, Université Paris-Est ANSES Alfort, Maisons-Alfort, France.
authors.forename: E
authors.initials: E
authors.lastname: Breard
authors.affiliation: UMR 1161 ANSES/INRA/ENVA, Université Paris-Est ANSES Alfort, Maisons-Alfort, France.
authors.forename: C
authors.initials: C
authors.lastname: Viarouge
authors.affiliation: Unit of Viral Genetics and Biosafety, Anses, Laboratory of Ploufragan, Ploufragan, France.
authors.forename: H
authors.initials: H
authors.lastname: Quenault
authors.affiliation: Unit of Viral Genetics and Biosafety, Anses, Laboratory of Ploufragan, Ploufragan, France.
authors.forename: P
authors.initials: P
authors.lastname: Lucas
authors.affiliation: UMR 1161 ANSES/INRA/ENVA, Université Paris-Est ANSES Alfort, Maisons-Alfort, France.
authors.forename: C
authors.initials: C
authors.lastname: Sailleau
authors.affiliation: UMR 1161 ANSES/INRA/ENVA, Université Paris-Est ANSES Alfort, Maisons-Alfort, France.
authors.forename: S
authors.initials: S
authors.lastname: Zientara
authors.affiliation: USC ANSES "VECPAR" EA4688, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France.
authors.forename: D
authors.initials: D
authors.lastname: Augot
authors.affiliation: Instituto de Microbiología, Universidad San Francisco de Quito, Quito, Ecuador.
authors.forename: S
authors.identifier: http://orcid.org/0000-0003-0499-1262
authors.initials: S
authors.lastname: Zapata
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001819:Bluetongue / Q000453:epidemiology / Q000821:virology*; D001820:Bluetongue virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004484:Ecuador / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016036:Seroepidemiologic Studies; D065288:Serogroup; D012703:Serotyping; D013020:South America / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral
keywords: Bluetongue; Ecuador; Orbivirus; cattle; epizootic haemorrhagic disease