title: Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells.
abstract: Suspension-cultured cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK activation and ROS generation in rice suspension-cultured cells.
authors.affiliation: Graduate School of Agriculture, Kindai University, 3327-204 Nakamachi, Nara, 631-8505, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yamaguchi
authors.affiliation: Graduate School of Agriculture, Kindai University, 3327-204 Nakamachi, Nara, 631-8505, Japan. t-kawasaki@nara.kindai.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kawasaki
mesh_terms: D002478:Cells, Cultured; D002686:Chitin / Q000494:pharmacology*; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D012275:Oryza / Q000166:cytology* / Q000378:metabolism; D057865:Plant Immunity; D010940:Plant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D002686:Chitin; D020928:Mitogen-Activated Protein Kinases
keywords: Chitin; Immunity; MAPK; ROS; Rice