title: Towards the Full Realization of 2DE Power.
abstract: Here, approaches that allow disclosure of the information hidden inside and outside of two-dimensional gel electrophoresis (2DE) are described. Experimental identification methods, such as mass spectrometry of high resolution and sensitivity (MALDI-TOF MS and ESI LC-MS/MS) and immunodetection (Western and Far-Western) in combination with bioinformatics (collection of all information about proteoforms), move 2DE to the next level of power. The integration of these technologies will promote 2DE as a powerful methodology of proteomics technology.
authors.affiliation: Institute of Biomedical Chemistry, Pogodinskaya 10, Moscow 119121, Russia. snaryzhny@mail.ru.|B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad region, Gatchina 188300, Russia. snaryzhny@mail.ru.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Naryzhny
keywords: bioinformatics; mass-spectrometry; proteoforms; two-dimensional gel electrophoresis