title: MicroRNA screening identifies miR-134 as a regulator of poliovirus and enterovirus 71 infection.
abstract: MicroRNAs (miRNAs) regulate virus replication through multiple mechanisms. Poliovirus causes a highly debilitating disease and though global efforts to eradicate polio have sharply decreased polio incidence, unfortunately three countries (Afghanistan, Nigeria and Pakistan) remain polio-endemic. We hypothesize that understanding the host factors involved in polio replication will identify novel prophylactic and therapeutic targets against polio and related viruses. In this data set, employing genome wide screens of miRNA mimics and inhibitors, we identified miRNAs which significantly suppressed polio replication. Specifically, miR-134 regulates poliovirus replication via modulation of ras-related nuclear protein (RAN), an important component of the nuclear transport system. MiR-134 also inhibited other Picornaviridae viruses including EV71, a growing concern and a high priority for vaccination in Asian countries like China. These findings demonstrate a novel mechanism for miRNA regulation of poliovirus and other Picornaviridae viruses in host cells, and thereby may provide a novel approach in combating infection and a potential approach for the development of anti-Picornaviridae strategies.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Nichole Lynn
authors.initials: NL
authors.lastname: Orr-Burks
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Byoung-Shik
authors.initials: BS
authors.lastname: Shim
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Weilin
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Abhijeet A
authors.initials: AA
authors.lastname: Bakre
authors.affiliation: Proventus Bio, 220 Riverbend Rd, Athens, Georgia 30602, USA.
authors.forename: Jon
authors.initials: J
authors.lastname: Karpilow
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Tripp
mesh_terms: D002681:China; D029821:Enterovirus A, Human; D004769:Enterovirus Infections / Q000453:epidemiology / Q000235:genetics*; D015994:Incidence; D035683:MicroRNAs; D011051:Poliomyelitis / Q000453:epidemiology / Q000235:genetics*; D017955:Poliovirus; D014779:Virus Replication
chemical_list: C569273:MIRN134 microRNA, human; D035683:MicroRNAs