title: Genome-wide screening for highly discriminative SNPs for personal identification and their assessment in world populations.
abstract: The applications of DNA profiling aim to identify perpetrators, missing family members and disaster victims in forensic investigations. Single nucleotide polymorphisms (SNPs) based forensic applications are emerging rapidly with a potential to replace short tandem repeats (STRs) based panels which are now being used widely, and there is a need for a well-designed SNP panel to meet such challenge for this transition. Here we present a panel of 175 SNP markers (referred to as Fudan ID Panel or FID), selected from ∼3.6 million SNPs, for the application of personal identification. We optimized and validated FID panel using 729 Chinese individuals using a next generation sequencing (NGS) technology. We showed that the SNPs in the panel possess very high heterozygosity as well as low within- and among-continent differentiations, enabling FID panel exhibit discrimination power in both regional and worldwide populations, with the average match probabilities ranging from 4.77×10-71to 1.06×10-64across 54 world populations. With the advent of biomedical research, the SNPs connecting physical anthropological, physiological, behavioral and phenotypic traits will be eventually added to the forensic panels that will revolutionize criminal investigation.
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Mingying
authors.initials: M
authors.lastname: Xia
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China; Fudan-Taizhou Institute of Health Sciences, Jiangsu, 225300, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China; Fudan-Taizhou Institute of Health Sciences, Jiangsu, 225300, China.
authors.forename: Jiucun
authors.initials: J
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China; Fudan-Taizhou Institute of Health Sciences, Jiangsu, 225300, China.
authors.forename: Daru
authors.initials: D
authors.lastname: Lu
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Pan
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Jiang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Forensic Medicine, Shanghai Medicine College, Fudan University, Shanghai, 200000, China.
authors.forename: Ziqin
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Shanghai Public Security Bureau-Fudan University Joint Laboratory of Human Biology and Forensic Techniques for Crime Scenes, Shanghai Research Institute of Criminal Science and Technology, Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Shanghai, 200000, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ping
authors.affiliation: Shanghai Public Security Bureau-Fudan University Joint Laboratory of Human Biology and Forensic Techniques for Crime Scenes, Shanghai Research Institute of Criminal Science and Technology, Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Shanghai, 200000, China.
authors.forename: Huaigu
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Shanghai Public Security Bureau-Fudan University Joint Laboratory of Human Biology and Forensic Techniques for Crime Scenes, Shanghai Research Institute of Criminal Science and Technology, Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Shanghai, 200000, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China; Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Nantong Bureau of Public Safety, Jiangsu, 226000, China.
authors.forename: Dongtao
authors.initials: D
authors.lastname: Jia
authors.affiliation: National Institute of Forensics, Ministry of Justice, Shanghai, 200000, China.
authors.forename: Chengtao
authors.initials: C
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China; Medical College of Inner Mongolia, Ulaanbaatar, Autonomous Region of Inner Mongolia, 010000, China.
authors.forename: Rile
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 200000, China.
authors.forename: Hongzhou
authors.initials: H
authors.lastname: Lu
authors.affiliation: China-Japan Friendship Hospital, Jilin University, Changchun, Jilin, 130000, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Cancer Hospital, Changchun, Jilin, 130000, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Biology and Geography, Qinghai Normal University, Xining, Qinghai, 810000, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Mi
authors.affiliation: School of Public Health, Shandong University, Jinan, Shandong, 250000, China.
authors.forename: Fuzhong
authors.initials: F
authors.lastname: Xue
authors.affiliation: Bureau of Public Safety, Lhasa, Autonomous Region of Tibet, 850000, China.
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Su
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China; Fudan-Taizhou Institute of Health Sciences, Jiangsu, 225300, China. Electronic address: lijin@fudan.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Developmental Biology and School of Life Sciences, Fudan University, Shanghai, 200438, China; Fudan-Taizhou Institute of Health Sciences, Jiangsu, 225300, China. Electronic address: lishilin@fudan.edu.cn.
authors.forename: Shilin
authors.initials: S
authors.lastname: Li
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D005828:Genetics, Population; D060005:Genotyping Techniques; D060148:HapMap Project; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D020641:Polymorphism, Single Nucleotide
keywords: Forensics; Ion Torrent; Next generation sequencing; Personal identification; Single nucleotide polymorphisms