title: Evaluation of Laser Speckle Contrast Imaging for the Assessment of Oral Mucosal Blood Flow following Periodontal Plastic Surgery: An Exploratory Study.
abstract: The laser speckle contrast imaging (LSCI) is proved to be a reliable tool in flap monitoring in general surgery; however, it has not been evaluated in oral surgery yet. We applied the LSCI to compare the effect of a xenogeneic collagen matrix (Geistlich Mucograft®) to connective tissue grafts (CTG) on the microcirculation of the modified coronally advanced tunnel technique (MCAT) for gingival recession coverage. Gingival microcirculation and wound fluid were measured before and after surgery for six months at twenty-seven treated teeth. In males, the flap microcirculation was restored within 3 days for both grafts followed by a hyperemic response. During the first 8 days the blood flow was higher at xenogeneic graft comparing to the CTG. In females, the ischemic period lasted for 7-12 days depending on the graft and no hyperemic response was observed. Females had more intense and prolonged wound fluid production. The LSCI method is suitable to capture the microcirculatory effect of the surgical intervention in human oral mucosa. The application of xenogeneic collagen matrices as a CTG substitute does not seem to restrain the recovery of graft bed circulation. Gender may have an effect on postoperative circulation and inflammation.
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, Szentkirályi Utca 47, Budapest 1088, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Molnár
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Semmelweis University, Szentkirályi Utca 47, Budapest 1088, Hungary.
authors.forename: Bálint
authors.initials: B
authors.lastname: Molnár
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, Szentkirályi Utca 47, Budapest 1088, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Lohinai
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, Szentkirályi Utca 47, Budapest 1088, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Tóth
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Faculty of Medicine, Semmelweis University, Tűzoltó Utca 37-47, Budapest 1094, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Benyó
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Faculty of Medicine, Semmelweis University, Tűzoltó Utca 37-47, Budapest 1094, Hungary.
authors.forename: Laszló
authors.initials: L
authors.lastname: Hricisák
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Semmelweis University, Szentkirályi Utca 47, Budapest 1088, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Windisch
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, Szentkirályi Utca 47, Budapest 1088, Hungary.
authors.forename: János
authors.identifier: 0000-0002-6183-7598
authors.initials: J
authors.lastname: Vág
mesh_terms: D000328:Adult; D001794:Blood Pressure; D005260:Female; D005889:Gingival Recession / Q000601:surgery; D006801:Humans; D021621:Imaging, Three-Dimensional; D007834:Lasers; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000098:blood supply* / Q000601:surgery*; D010519:Periodontium / Q000601:surgery*; D012039:Regional Blood Flow; D013518:Surgery, Plastic; D013997:Time Factors; D014945:Wound Healing