title: Antimicrobial Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Child Patients of High-Risk Wards in Shenzhen, China.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) strains are responsible for high rates of mortality and thus pose a substantial burden to public health worldwide. Here, we investigated the antimicrobial susceptibility and molecular characteristics of MRSA isolated from child patients at Shenzhen Children's Hospital. We characterized 140 MRSA strains through antimicrobial susceptibility testing. We further performed spa typing, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) analysis, pvl gene analysis, and pulsed-field gel electrophoresis (PFGE). The analyzed MRSA strains were found to be sensitive to most non-β-lactam antimicrobial agents. Sequence type (ST) 59 was found to be the most common MLST lineage (54.3%). Most MRSA isolates belonged to the SCCmec IV (64.3%) and V (22.8%) types. The MRSA-ST59-SCCmec IV-t437 clone was the most predominant strain that infected 28.6% of all patients studied. Moreover, 50.7% of MRSA isolates were found to be pvl-positive. We report preliminary data on the prevalence and distribution of MRSA genotypes in Shenzhen Children's Hospital. We characterized MRSA colonization dynamics in child patients in China, and our findings can serve as the basis for the development of strategies to prevent MRSA infection and transmission.
authors.affiliation: First Affiliated Hospital of Jinan University.|Shenzhen Children's Hospital.
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Shenzhen Children's Hospital.
authors.forename: Feiqiu
authors.initials: F
authors.lastname: Wen
authors.affiliation: Shenzhen Children's Hospital.
authors.forename: Yuejie
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Shenzhen Children's Hospital.
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Center for Disease Control and Prevention (CDC).
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Center for Disease Control and Prevention (CDC).
authors.forename: Renli
authors.initials: R
authors.lastname: Zhang
mesh_terms: D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D006776:Hospitals, Pediatric; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058889:Molecular Typing; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*
keywords: antimicrobial resistance; child patient; methicillin-resistant Staphylococcus aureus (MRSA); molecular characteristics