title: Titanium Dioxide Modulation of the Contractibility of Visceral Smooth Muscles In Vivo.
abstract: Electronic scanning microscopy was used in the work to obtain the image and to identify the sizes of titanium dioxide (TiO2) nanoparticles 21 ± 5 nm. The qualitative and quantitative elemental analysis of the preparations of the caecum, antrum, myometrium, kidneys, and lungs of the rats, burdened with titanium dioxide, was also performed. It was established using the tenzometric method in the isometric mode that the accumulation of titanium dioxide in smooth muscles of the caecum resulted in the considerable, compared to the control, increase in the frequency of their spontaneous contractions, the decrease in the duration of the contraction-relaxation cycle, and the decrease in the indices of muscle functioning efficiency (the index of contractions in Montevideo units (MU) and the index of contractions in Alexandria units (AU)). In the same experimental conditions, there was not the increase, but the decrease in the frequency of spontaneous contractions, the duration of the contraction-relaxation cycle, and the increase in MU and AU indices in the smooth muscles of myometrium (in the group of rats, burdened with TiO2for 30 days). It was also determined that TiO2modulates both the mechanisms of the input of extracellular Ca2+ions and the mechanisms of decreasing the concentration of these cations in smooth muscle cells of the caecum during the generation of the high potassium contraction. In these conditions, there is a considerable increase in the normalized maximal velocity of the contraction phase and the relaxation phase. It was demonstrated in the work that titanium dioxide also changes the cholinergic excitation in these muscles. The impact of titanium dioxide in the group of rats, burdened with TiO2, was accompanied with a considerable impairment of the kinetics of forming the tonic component of the oxytocin-induced contraction of the smooth muscles of myometrium.
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp. 4g, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine. otsymbal@bigmir.net.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Tsymbalyuk
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp. 4g, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Naumenko
authors.affiliation: NanoMedTech LLC, 68, Antonovycha Str, Kyiv, 03680, Ukraine.
authors.forename: Oleksandr O
authors.initials: OO
authors.lastname: Rohovtsov
authors.affiliation: NanoMedTech LLC, 68, Antonovycha Str, Kyiv, 03680, Ukraine.
authors.forename: Mykola A
authors.initials: MA
authors.lastname: Skoryk
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp. 4g, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine.
authors.forename: Ivan S
authors.initials: IS
authors.lastname: Voiteshenko
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp. 4g, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine.
authors.forename: Valeriy A
authors.initials: VA
authors.lastname: Skryshevsky
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, 2, korp. 4g, Pr. Akademika Hlushkova, Kyiv, 03022, Ukraine.
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Davydovska
keywords: Acetylcholine; Caecum; Contractile activity; Diazoxide; Elemental analysis; Glibenclamide; High potassium contraction; Myometrium; Oxytocin; TiO2