title: Actinomadura alkaliterrae sp. nov., isolated from an alkaline soil.
abstract: A polyphasic study was undertaken to establish the taxonomic status of an Actinomadura strain isolated from the margin of a saline, alkaline lake in Central Anatolia, Turkey. Strain D310ATTwas shown to have chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Actinomadura such as hooked or irregular spiral spore chains, meso-diaminopimelic acid as the major cell wall diaminopimelic acid, and diphosphatidylglycerol and phosphatidylinositol as major polar lipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain D310ATTis closely, albeit loosely, associated with Actinomadura darangshiensis DLS-70Twith 97.2% sequence similarity, but was readily separated from the latter using diverse phenotypic properties. Consequently, the isolate is considered to represent a new species of Actinomadura for which the name Actinomadura alkaliterrae sp. nov. is proposed, with the type strain D310ATT(=DSM 101185T= KCTC 39657T).
authors.affiliation: Department of Biology, Faculty of Science and Arts Ondokuz Mayis University, Samsun, Turkey. hilal.ay@omu.edu.tr.
authors.forename: Hilal
authors.initials: H
authors.lastname: Ay
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Imen
authors.initials: I
authors.lastname: Nouioui
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Del Carmen Montero-Calasanz
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Lorena
authors.initials: L
authors.lastname: Carro
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
authors.affiliation: Instituto de Recursos Naturales y Agrobiologia de Salamanca, Consejo Superior de Investigaciones Cientificas (IRNASA-CSIC), Salamanca, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Igual
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Çetin
authors.affiliation: Department of Biology, Faculty of Science and Arts Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Şahin
authors.affiliation: Department of Biology, Faculty of Science and Arts Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Kamil
authors.initials: K
authors.lastname: Işık
mesh_terms: D000191:Actinomycetaceae / Q000302:isolation & purification / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial; D003960:Diaminopimelic Acid / Q000378:metabolism; D005227:Fatty Acids; D009693:Nucleic Acid Hybridization; D010743:Phospholipids; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D014421:Turkey
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D003960:Diaminopimelic Acid
keywords: Alkaline soil; Lake Acıgöl; Phenotyping; Phylogeny; Polyphasic taxonomy