title: Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice.
abstract: Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous type 2 diabetic db/db mice by using the cDNA microarray. Compared with control db/m mice, the db/db mice exhibited marked increases in body weight, kidney weight, and urinary albumin excretion. Renal histological analysis revealed mesangial expansion and thickness of the basement membrane in the kidney of the db/db mice. A total of 355 differentially expressed genes (DEGs) were identified by microarray analysis. Pathway enrichment analysis suggested that biological oxidation, bile acid metabolism, and steroid hormone synthesis were the 3 major significant pathways. The top 10 hub genes were selected from the constructed PPI network of DEGs, includingCcnb2andNr1i2, which remained largely unclear in DN. We believe that our study can help elucidate the molecular mechanisms underlying DN.
authors.affiliation: Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Haojun
authors.identifier: 0000-0002-5993-5260
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Medical Research Center, International Science and Technology Cooperation Base of Geriatric Medicine, North China University of Science and Technology, Tangshan, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yan
authors.affiliation: Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Ping
authors.identifier: 0000-0002-3315-2276
authors.initials: P
authors.lastname: Li
mesh_terms: D000419:Albuminuria / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000502:physiology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D059467:Transcriptome