title: A Proteomic Study ofClavibacter MichiganensisSubsp.MichiganensisCulture Supernatants.
abstract: Clavibacter michiganensis, subsp.michiganensisis a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors ofC. michiganensissubsp.michiganensisand host-pathogen interactions on a molecular level are rather limited. In the study presented here, the proteome of culture supernatants fromC. michiganensissubsp.michiganensisNCPPB382 was analyzed. In total, 1872 proteins were identified in M9 and 1766 proteins in xylem mimicking medium. Filtration of supernatants before protein precipitation reduced these to 1276 proteins in M9 and 976 proteins in the xylem mimicking medium culture filtrate. The results obtained indicate thatC. michiganensissubsp.michiganensisreacts to a sucrose- and glucose-depleted medium similar to the xylem sap by utilizing amino acids and host cell polymers as well as their degradation products, mainly peptides, amino acids and various C5 and C6 sugars. Interestingly, the bacterium expresses the previously described virulence factors Pat-1 and CelA not exclusively after host cell contactin plantabut already in M9 minimal and xylem mimicking medium.
authors.affiliation: Department Biologie Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. ehiery@biologie.uni-erlangen.de.
authors.forename: Eva
authors.initials: E
authors.lastname: Hiery
authors.affiliation: Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität Bochum, Universitätsstr. 150, 44801 Bochum, Germany. ansgar.poetsch@ruhr-uni.bochum.de.
authors.forename: Ansgar
authors.initials: A
authors.lastname: Poetsch
authors.affiliation: Department Biologie Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. tanja.moosbauer@fau.de.
authors.forename: Tanja
authors.initials: T
authors.lastname: Moosbauer
authors.affiliation: Department Biologie Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. bushra.amin@fau.de.
authors.forename: Bushra
authors.initials: B
authors.lastname: Amin
authors.affiliation: Department Biologie Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. joerg.hofmann@fau.de.
authors.forename: Jörg
authors.initials: J
authors.lastname: Hofmann
authors.affiliation: Department Biologie Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. andreas.burkovski@fau.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Burkovski
keywords: Clavibacter; canker; plant pathogen; secretome; wilt; xylem mimicking medium; xylem sap