title: Simultaneous Profiling of Lysoglycerophospholipids in Rice (Oryza sativa L.) Using Direct Infusion-Tandem Mass Spectrometry with Multiple Reaction Monitoring.
abstract: White rice is the final product after the hull and bran layers have been removed during the milling process. Although lysoglycerophospholipids (lysoGPLs) only occupy a small proportion in white rice, they are essential for evaluating rice authenticity and quality. In this study, we developed a high-throughput and targeted lipidomics approach that involved direct infusion-tandem mass spectrometry with multiple reaction monitoring to simultaneously profile lysoGPLs in white rice. The method is capable of characterizing 17 lysoGPLs within 1 min. In addition, unsupervised and supervised analyses exhibited a considerably large diversity of lysoGPL concentrations in white rice from different origins. In particular, a classification model was built using identified lysoGPLs that can differentiate white rice from Korea, China, and Japan. Among the discriminatory lysoGPLs, for the lysoPE(16:0) and lysoPE(18:2) compositions, there were relatively small within-group variations, and they were considerably different among the three countries. In conclusion, our proposed method provides a rapid, high-throughput, and comprehensive format for profiling lysoGPLs in rice samples.
authors.affiliation: Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University , Seoul 08826, Republic of Korea.
authors.forename: Dong Kyu
authors.initials: DK
authors.lastname: Lim
authors.affiliation: National Institute of Agricultural Sciences, Rural Development Administration , Jeonju 54875, Republic of Korea.
authors.forename: Changyeun
authors.initials: C
authors.lastname: Mo
authors.affiliation: Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University , Seoul 08826, Republic of Korea.
authors.forename: Nguyen Phuoc
authors.initials: NP
authors.lastname: Long
authors.affiliation: National Institute of Agricultural Sciences, Rural Development Administration , Jeonju 54875, Republic of Korea.
authors.forename: Giyoung
authors.initials: G
authors.lastname: Kim
authors.affiliation: Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University , Seoul 08826, Republic of Korea.|Plant Genomics and Breeding Institute, Seoul National University , Seoul 08826, Republic of Korea.
authors.forename: Sung Won
authors.identifier: http://orcid.org/0000-0001-7161-4737
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D020404:Glycerophospholipids / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020404:Glycerophospholipids; D010936:Plant Extracts
keywords: Oryza sativa L.; direct infusion-mass spectrometry; discriminatory marker; lysoglycerophospholipid; partial least-squares discriminant analysis