title: Comparison of HbA1c detection in whole blood and dried blood spots using an automated ion-exchange HPLC system.
abstract: OBJECTIVE
  Hemoglobin A1c (HbA1c) is a widely recognized analyte for diagnosing and monitoring diabetes. Dried blood spot (DBS) constitutes a useful alternative to blood collection by venipuncture. Analytical and clinical validation of DBS use is, however, necessary before implementation. Results/methodology: HbA1c levels from whole blood or DBS from a cohort patients with diabetes were compared. DBS specimens were stable at ambient temperature. HbA1c detection on DBS was accurate, robust, and the correlation and agreement with whole blood values was excellent.
  
  
  CONCLUSIONS
  This study provides for the first time a complete method comparison and validation under the ISO15189 guideline using an automated HPLC system. This approach constitutes, therefore, a useful tool for diagnosing diabetes.
authors.affiliation: Université de Montpellier, CHU de Montpellier, Laboratoire de Biochimie Protéomique Clinique, INSERM U1183, F-34000 France.|CHU Montpellier, Laboratoire de Virologie, Hôpital Lapeyronie, Montpellier, F-34000 France; INSERM U1058, Montpellier, F-34000 France.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Maleska
authors.affiliation: Université de Montpellier, CHU de Montpellier, Laboratoire de Biochimie Protéomique Clinique, INSERM U1183, F-34000 France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
authors.affiliation: Université de Montpellier, CHU de Montpellier, Laboratoire de Biochimie Protéomique Clinique, INSERM U1183, F-34000 France.
authors.forename: Elise
authors.initials: E
authors.lastname: Casteleyn
authors.affiliation: Endocrinology-Diabetology-Nutrition Department, University Hospital, PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214, Montpellier, France.
authors.forename: Orianne
authors.initials: O
authors.lastname: Villard
authors.affiliation: CHU Montpellier, Laboratoire de Virologie, Hôpital Lapeyronie, Montpellier, F-34000 France; INSERM U1058, Montpellier, F-34000 France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Ducos
authors.affiliation: Endocrinology-Diabetology-Nutrition Department, University Hospital, PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214, Montpellier, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Avignon
authors.affiliation: Endocrinology-Diabetology-Nutrition Department, University Hospital, PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214, Montpellier, France.
authors.forename: Ariane
authors.initials: A
authors.lastname: Sultan
authors.affiliation: Université de Montpellier, CHU de Montpellier, Laboratoire de Biochimie Protéomique Clinique, INSERM U1183, F-34000 France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
mesh_terms: D000328:Adult; D000368:Aged; D001331:Automation; D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000473:pathology; D059788:Dried Blood Spot Testing; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D055550:Protein Stability
chemical_list: D015415:Biomarkers; D006442:Glycated Hemoglobin
keywords: DBS; HPLC; HbA1c; ISO15189; clinical biology; dried blood spot; glycated hemoglobin; ion exchange; method validation