title: STABILIZED1 Modulates Pre-mRNA Splicing for Thermotolerance.
abstract: High-temperature stress often leads to differential RNA splicing, thus accumulating different types and/or amounts of mature mRNAs in eukaryotic cells. However, regulatory mechanisms underlying plant precursor mRNA (pre-mRNA) splicing in the environmental stress conditions remain elusive. Herein, we describe that a U5-snRNP-interacting protein homolog STABILIZED1 (STA1) has pre-mRNA splicing activity for heat-inducible transcripts includingHEAT STRESS TRANSCRIPTION FACTORs and variousHEAT SHOCK PROTEINs for the establishment of heat stress tolerance in Arabidopsis (Arabidopsis thaliana). Our cell-based splicing reporter assay demonstrated STA1 acts on pre-mRNA splicing for specific subsets of stress-related genes. Cellular reconstitution of heat-inducible transcription cascades supported the view that STA1-dependent pre-mRNA splicing plays a role in DREB2A-dependentHSFA3expression for heat-responsive gene expression. Further genetic analysis with a loss-of-function mutantsta1-1,STA1-expressing transgenic plants in Col background, andSTA1-expressing transgenic plants in thesta1-1background verified that STA1 is essential in expression of necessary genes includingHSFA3for two-step heat stress tolerance in plants. However, constitutive overexpression of the cDNA version ofHSFA3in thesta1-1background is unable to execute plant heat stress tolerance insta1-1Consistently our global target analysis of STA1 showed that its splicing activity modulates a rather broad range of gene expression in response to heat treatment. The findings of this study reveal that heat-inducible STA1 activity for pre-mRNA splicing serves as a molecular regulatory mechanism underlying the plant stress tolerance to high-temperature stress.
authors.affiliation: Division of Life Science, College of Life Science and Biotechnology, Korea University, Seoul, Korea (G.-D.K.; Y.-H.C.; S.-D.Y.); and.|Department of Life Science, Sogang University, Seoul, Korea (B.-H.L.).
authors.forename: Geun-Don
authors.identifier: 0000-0002-3580-2471
authors.initials: GD
authors.lastname: Kim
authors.affiliation: Division of Life Science, College of Life Science and Biotechnology, Korea University, Seoul, Korea (G.-D.K.; Y.-H.C.; S.-D.Y.); and.|Department of Life Science, Sogang University, Seoul, Korea (B.-H.L.).
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Division of Life Science, College of Life Science and Biotechnology, Korea University, Seoul, Korea (G.-D.K.; Y.-H.C.; S.-D.Y.); and.|Department of Life Science, Sogang University, Seoul, Korea (B.-H.L.).
authors.forename: Byeong-Ha
authors.identifier: 0000-0001-7633-3264
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Division of Life Science, College of Life Science and Biotechnology, Korea University, Seoul, Korea (G.-D.K.; Y.-H.C.; S.-D.Y.); and sangdong@koea.ac.kr.|Department of Life Science, Sogang University, Seoul, Korea (B.-H.L.) sangdong@koea.ac.kr.
authors.forename: Sang-Dong
authors.identifier: 0000-0002-3733-2354
authors.initials: SD
authors.lastname: Yoo
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D008957:Models, Genetic; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological; D000071436:Thermotolerance / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; D012322:RNA Precursors; C519660:STA1 protein, Arabidopsis