title: Synthesis and characterization of an injectable hyaluronic acid-polyaspartylhydrazide hydrogel.
abstract: The hydrogel produced by the reaction between a hyaluronic acid derivative (HAALD) and α,β-polyaspartylhydrazide (PAHy) hydrogel was used for lacrimal duct studies. In order to improve the mechanical properties of HAALD-PAHy hydrogel, glutaraldehyde (GA) was used as a candidate to increase the mechanical properties of the hydrogel. The optimum mass ratio of the GA and PAHy was 1:50. HAALD-PAHy and HAALD-PAHy-GA50 were both synthesized in PBSA solution and characterized by different methods including gel content and swelling, rheological analysis, in vitro degradation and in vivo degradation via rheological analysis. The storage modulus (G') of the HAALD-PAHy-GA50 hydrogel reached 3800 Pa, i.e. (2.9±0.3 times higher than for HAALD-PAHy). The in vitro cytotoxicity test revealed that HAALD-PAHy-GA50 have a good biocompatibility and in vivo animal testing concluded that HAALD-PAHy-GA50 remains in the rabbit's lacrimal duct for 28 days.
authors.affiliation: Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, P.R. China.
authors.forename: Qiuchi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, P.R. China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.affiliation: Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015352:Dry Eye Syndromes / Q000188:drug therapy*; D005612:Freeze Drying; D005976:Glutaral / Q000138:chemical synthesis / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000494:pharmacology*; D020100:Hydrogels / Q000138:chemical synthesis / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D011108:Polymers; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides; D011108:Polymers; C442699:poly(aspartylhydrazide); D006820:Hyaluronic Acid; D005976:Glutaral
keywords: Hyaluronic acid; glutaraldehyde; hydrogel; plugs; polyaspartic acid