title: Constituent analysis of the ethanol extracts of Chimonanthus nitens Oliv. leaves and their inhibitory effect on α-glucosidase activity.
abstract: The ethanol extracts of Chimonanthus nitens Oliv. leaves were prepared sequentially by ethanol gradient elution and tested for their α-glucosidase inhibitory. The fraction of 50% ethanol eluate (EE) exhibited the notable inhibition with IC50of 0.376mg/mL. Also, 50% EE was chemically characterized by liquid chromatography-mass spectrometry (LC-MS) analysis. Eight compounds including rutin (1), hyperin (2), isoquercitrin (3), luteoloside (4), astragalin (6), quercetin (13), naringenin (14), kaempferol (15) were identified by compared with standard substances as well as proper luteolin-5-O-glucoside (5), kaempferol-7-O-rhamnoside (9), 5,7,8-trihydroxy-2-methoxyl-flavone-7-O-glucoside (10), kaempferol-7-O-acetyl-galactoside (11). The experiments of ultra-filtration combined with liquid chromatography-mass spectrometry (UF-LC-MS) guided quercetin and kaempferol as the key factors for 50% EE showing highly inhibitory activity on α-glucosidase. Quercetin and kaempferol inhibited yeast α-glucosidase in a mixed-type manner with IC50of 66.8 and 109μg/mL, respectively. These results would provide theoretical underpinning for the C. nitens Oliv. leaves ethanol extracts used as nutraceutical health supplement in the management of type 2 diabetes.
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Kehui
authors.initials: K
authors.lastname: Ouyang
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Hu
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address: wwjun9999@sina.com.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
mesh_terms: D031237:Calycanthaceae / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000178:diet therapy; D000431:Ethanol / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000096:biosynthesis*
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D044949:Kaempferols; D010936:Plant Extracts; D000431:Ethanol; C006552:kaempferol; D011794:Quercetin; D000520:alpha-Glucosidases
keywords: Chimonanthus nitens Oliv. leaves; Flavonoids; UF-LC–MS; α-Glucosidase inhibitor