title: The microRNA miR-124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium-binding protein A4 (S100A4).
abstract: S100 calcium-binding protein A4 (S100A4) induces proliferation and migration of vascular smooth muscle cells (VSMCs). We aimed to find the microRNA regulating S100A4 expression. S100A4 transcripts are abruptly increased in the acute phase of carotid arterial injury 1 day later (at day 1) but gradually decreases at days 7 and 14. Bioinformatics analysis reveals that miR-124 targets S100A4. VSMC survival is attenuated by miR-124 mimic but increased by miR-124 inhibitor. miR-124 decreases immediately after carotid arterial injury but dramatically increases at days 7 and 14. miR-124 inhibitor-induced cell proliferation is blocked by S100A4 siRNA, whereas miR-124-induced cell death is recovered by S100A4. Our findings suggest that miR-124 is a novel regulator of VSMC proliferation and may play a role in the development of neointimal proliferation.
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea.|Basic Research Laboratory for Cardiac Remodeling, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Nakwon
authors.initials: N
authors.lastname: Choe
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea.|Basic Research Laboratory for Cardiac Remodeling, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Duk-Hwa
authors.initials: DH
authors.lastname: Kwon
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea.|Basic Research Laboratory for Cardiac Remodeling, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Sera
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Cardiology, Chonnam National University Hospital, Gwangju, Korea.
authors.forename: Yong Sook
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Basic Research Laboratory for Cardiac Remodeling, Chonnam National University Medical School, Gwangju, Korea.|Department of Biochemistry, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Young-Kook
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Basic Research Laboratory for Cardiac Remodeling, Chonnam National University Medical School, Gwangju, Korea.|Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, Korea.
authors.forename: Jaetaek
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Cardiology, Chonnam National University Hospital, Gwangju, Korea.
authors.forename: Youngkeun
authors.initials: Y
authors.lastname: Ahn
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea.|Medical Research Center for Gene Regulation, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Gwang H
authors.initials: GH
authors.lastname: Eom
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea.|Basic Research Laboratory for Cardiac Remodeling, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Kook
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D020212:Carotid Artery Injuries / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D058426:Neointima / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071999:S100 Calcium-Binding Protein A4 / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
chemical_list: D020413:3' Untranslated Regions; C548144:MIRN124 microRNA, rat; D035683:MicroRNAs; D000071999:S100 Calcium-Binding Protein A4; C499412:S100a4 protein, rat
keywords: 
  miR-124
  ; S100A4; carotid artery injury; neointima; vascular smooth muscle cell proliferation