title: Knockdown of DDX5 Inhibits the Proliferation and Tumorigenesis in Esophageal Cancer.
abstract: DEAD (Asp-Glu-Ala-Asp) box protein 5 (DDX5), a prototypical member of the DEAD/H-box protein family, has been involved in several human malignancies. However, the expression and biological role of DDX5 in esophageal cancer (EC) remain largely unknown. In this study, we examined the role of DDX5 in regulating EC cell proliferation and tumorigenesis and explored its possible molecular mechanism. We found that DDX5 was overexpressed in human EC cell lines. In addition, knockdown of DDX5 significantly inhibited the proliferation of EC cells in vitro and the growth of EC xenografts in vivo. Knockdown of DDX5 also suppressed the migration/invasion and epithelial-to-mesenchymal transition (EMT) phenotype in EC cells. Furthermore, we observed that knockdown of DDX5 inhibited the expression of β-catenin, c-Myc, and cyclin D1 in EC cells. In conclusion, our findings provide the first evidence that siRNA-DDX5 inhibited the proliferation and invasion of EC cells through suppressing the Wnt/β-catenin signaling pathway. Therefore, DDX5 may be a novel potential therapeutic target for the prevention and treatment of EC.
authors.forename: Zhenchuan
authors.initials: Z
authors.lastname: Ma
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.forename: Yurui
authors.initials: Y
authors.lastname: Guo
authors.forename: Ranran
authors.initials: R
authors.lastname: Kong
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Ma
authors.forename: Liangzhang
authors.initials: L
authors.lastname: Sun
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Shaomin
authors.initials: S
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jiantao
authors.initials: J
authors.lastname: Jiang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Qiao
authors.forename: Yao
authors.initials: Y
authors.lastname: Cheng
authors.forename: Danjie
authors.initials: D
authors.lastname: Zha
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000235:genetics; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008807:Mice, Inbred BALB C; D060449:Wnt Signaling Pathway / Q000235:genetics; D023041:Xenograft Model Antitumor Assays; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D051176:beta Catenin; C506749:Ddx5 protein, human; D053487:DEAD-box RNA Helicases