title: In-vitroStudies onCalotropis proceraLeaf Extracts as Inhibitors of Key Enzymes Linked to Diabetes Mellitus.
abstract: The side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. Therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential ofCalotropis proceraleaf, as well as its possible mode of inhibiting these enzymes. Acetone, aqueous and ethanolic extracts ofC. proceraleaf was subjected to standard enzymes' inhibitory assayin-vitrousing porcine pancreatic α-amylase and rat intestinal α-glucosidase. Results obtained showed that out of all the extracts tested, ethanolic and aqueous extracts possessed the best inhibition of α-amylase (IC507.80 mg/mL) and α-glucosidase (3.25 mg/mL) respectively. The kinetic analysis of the mode of inhibition of these enzymes by the leaf extracts ofC. procera, revealed that these extracts inhibited both enzymes in a non-competitive manner. It is speculated that the α-amylase and α-glucosidase inhibitory properties of leaf extracts ofC. proceramay be due to the presence of some phytochemicals such as flavonoids, tannins and saponins in the plant. It can be concluded from this study that theCalotropis proceraextracts could serve as source of antidiabetic agents which may act through the inhibition of carbohydrate hydrolyzing enzymes, α-amylase and α-glucosidase.
authors.affiliation: Department of Biochemistry, Faculty of Science, Lagos State University, PMB 0001, Ojo, Lagos, Nigeria.
authors.forename: Mutiu Idowu
authors.initials: MI
authors.lastname: Kazeem
authors.affiliation: Department of Biochemistry, Faculty of Science, Lagos State University, PMB 0001, Ojo, Lagos, Nigeria.
authors.forename: Ayuva Mercy
authors.initials: AM
authors.lastname: Mayaki
authors.affiliation: Department of Biochemistry, Faculty of Science, Lagos State University, PMB 0001, Ojo, Lagos, Nigeria.
authors.forename: Bimpe Folashade
authors.initials: BF
authors.lastname: Ogungbe
authors.affiliation: Department of Biochemistry, Faculty of Science, Lagos State University, PMB 0001, Ojo, Lagos, Nigeria.
authors.forename: Anthony Babajide
authors.initials: AB
authors.lastname: Ojekale
keywords: A-amylase; A-glucosidase; Calotropis procera; Diabetes mellitus; Tannins