title: Versatile transduction scheme based on electrolyte-gated organic field-effect transistor used as immunoassay readout system.
abstract: We report on an innovative heterogeneous bisphenol A (BPA) immunoassay based on an electrolyte-gated organic field-effect transistor whose organic semiconductor is poly(2,5-bis(3-tetradecylthiophen-2-yl)thieno[3,2-b]thiophene) co-crystallized with an alkyl derivative of bisphenol A. A decrease of the transistor output current is first observed upon antibody specific binding onto the organic semiconductor. Upon bisphenol A addition, the competitive dissociation of the antibody from the semiconductor surface leads to an opposite increase of the output current. We present here a proof-of-concept for bisphenol A detection; the device could be readily adapted to other small organic molecules of interest and is a promising tool for simple, low-cost, portable and easy-to-use biosensors.
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: piro@univ-paris-diderot.fr.
authors.forename: Benoît
authors.initials: B
authors.lastname: Piro
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: wangdengjun2005@yahoo.com.
authors.forename: Dengjun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: dihia.benaoudia@gmail.com.
authors.forename: Dihia
authors.initials: D
authors.lastname: Benaoudia
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: alexandra.tibaldi@gmail.com.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Tibaldi
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: guillaume.anquetin@univ-paris-diderot.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Anquetin
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: vincent.noel@univ-paris-diderot.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Noël
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: steeve.reisberg@univ-paris-diderot.fr.
authors.forename: Steeve
authors.initials: S
authors.lastname: Reisberg
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: giorgio.mattana@univ-paris-diderot.fr.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mattana
authors.affiliation: Smith College, Northampton, MA 01063, USA. Electronic address: bejackson@smith.edu.
authors.forename: Brianna
authors.initials: B
authors.lastname: Jackson
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D001559:Benzhydryl Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D004573:Electrolytes / Q000737:chemistry*; D004867:Equipment Design; D007118:Immunoassay; D010636:Phenols / Q000032:analysis*; D013876:Thiophenes / Q000737:chemistry; D014173:Transistors, Electronic / Q000191:economics; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D055910:Antibodies, Immobilized; D001559:Benzhydryl Compounds; D004573:Electrolytes; D010636:Phenols; D013876:Thiophenes; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Electrolyte-Gated OFET; Immunosensor; Label-free