title: Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation.
abstract: Bagging is an efficient method to improve fruit colour development. This work reported a transcriptome analysis using bagging-treated red Chinese sand pear peels. In total, 8,870 differentially expressed genes were further analysed by a weighted gene co-expression network analysis and early-, middle- and late light-responsive genes were identified. An annotation analysis revealed several pathways involved in the different responsive stages. The presence of LONG HYPOCOTLY 5, CRY-DASH and a CONSTANS-like transcription factors among the early light-responsive genes indicated the pivotal role of light, especially blue light, in the biological changes that occurred after bag removal. Other light-responsive transcription factors were also identified from the three light-responsive stages. In addition, the light-responsive pattern of anthocyanin biosynthetic genes differed among the biosynthetic steps. Although yeast-one hybrid assay showed that most of the structural genes were regulated by PpMYB10, their different temporal expressive pattern suggested that besides PpMYB10, other light-responsive transcriptional factors were also involved in the regulation of anthocyanin biosynthesis. In summary, our transcriptome analysis provides knowledge of the transcriptional regulatory network operating during light responses, which results in anthocyanin accumulation and other significant physiological changes in red Chinese sand pear peels after bag removal.
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, the Ministry of Agriculture of China, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
authors.forename: Songling
authors.initials: S
authors.lastname: Bai
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, the Ministry of Agriculture of China, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
authors.forename: Yongwang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, the Ministry of Agriculture of China, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Qian
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, the Ministry of Agriculture of China, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, the Ministry of Agriculture of China, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
authors.forename: Junbei
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, the Ministry of Agriculture of China, Hangzhou, 310058, Zhejiang Province, People's Republic of China.|Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Tao
authors.affiliation: Institute of Horticulture, Yunnan Academy of Agricultural Sciences, Kunming, 650205, Yunnan Province, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Horticulture, Yunnan Academy of Agricultural Sciences, Kunming, 650205, Yunnan Province, People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Shu
authors.affiliation: College of Horticulture, Northwest A & F University, Yangling, 712100, Shaanxi Province, People's Republic of China. afant@nwsuaf.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China. ywteng@zju.edu.cn.|The Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, the Ministry of Agriculture of China, Hangzhou, 310058, Zhejiang Province, People's Republic of China. ywteng@zju.edu.cn.|Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou, 310058, Zhejiang Province, People's Republic of China. ywteng@zju.edu.cn.
authors.forename: Yuanwen
authors.initials: Y
authors.lastname: Teng
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis* / Q000528:radiation effects; D003116:Color; D000069599:Crop Production; D005638:Fruit / Q000378:metabolism*; D020869:Gene Expression Profiling; D008027:Light; D031989:Pyrus / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D000872:Anthocyanins