title: Linear Multiepitope (Glyco)peptides for Type-Specific Serology of Herpes Simplex Virus (HSV) Infections.
abstract: Detection of type-specific antibodies is an important and essential part of accurate diagnosis, even in silent carriers of herpes simplex virus (HSV)-1 (oral) and HSV-2 (genital) infections. Serologic assays that identify HSV-1 and HSV-2 type-specific antibodies have been commercially available for more than a decade but often face problems related to cross-reactivity and similar issues. Attempts to identify type-specific peptide epitopes for use in serology for both HSV-1 and HSV-2 have been limited. We recently demonstrated epitope mapping of envelope glycoprotein G2 and identified a type-specific glycopeptide epitope that broadly recognized HSV-2 infected individuals. In the present work we have performed a comprehensive glycopeptide synthesis and microarray epitope mapping of 14 envelope proteins from HSV-1 and HSV-2, namely, gB, gC, gD, gE, gG, gH, and gI, using sera from HSV-1- and HSV-2-infected individuals and control sera. Several unique type-specific peptide epitopes with high sensitivity were identified and synthesized as one large linear multiepitope sequence using microwave-assisted solid-phase (glyco)peptide synthesis. Microarray validation with clinically defined HSV and Varicella Zoster (VZV) sera confirmed excellent cumulative specificities and sensitivities.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Copenhagen , Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Christian
authors.initials: C
authors.lastname: Risinger
authors.affiliation: Department of Chemistry, Faculty of Science, University of Copenhagen , Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Kasper K
authors.initials: KK
authors.lastname: Sørensen
authors.affiliation: Department of Chemistry, Faculty of Science, University of Copenhagen , Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Knud J
authors.identifier: 0000-0003-3525-5452
authors.initials: KJ
authors.lastname: Jensen
authors.affiliation: Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg , 41345 Gothenburg, Sweden.
authors.forename: Sigvard
authors.initials: S
authors.lastname: Olofsson
authors.affiliation: Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg , 41345 Gothenburg, Sweden.
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergström
authors.affiliation: Department of Chemistry, Faculty of Science, University of Copenhagen , Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Ola
authors.identifier: 0000-0003-4143-6276
authors.initials: O
authors.lastname: Blixt
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D000936:Antigen-Antibody Complex / Q000097:blood; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D003937:Diagnosis, Differential; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006023:Glycoproteins / Q000138:chemical synthesis / Q000276:immunology*; D006558:Herpes Genitalis / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D006561:Herpes Simplex / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D007074:Immunoglobulin G; D008872:Microwaves; D040081:Protein Array Analysis; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D007074:Immunoglobulin G
keywords: SPPS; glycoprotein; glycosylation; microarray; microwave synthesis; virology