title: Optimization of Herbicidin A Production in Submerged Culture ofStreptomyces scopuliridisM40.
abstract: Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture ofStreptomyces scopuliridisM40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to 180 μm, high volumetric production of herbicidin A was obtained with OUR values >137 mg O2/l·h. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of hyphae of 100-200 μm length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Ha
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Keon Jin
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Sang Il
authors.initials: SI
authors.lastname: Lee
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Gwak
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Tae-Woon
authors.initials: TW
authors.lastname: Kim
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Hak-Jong
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Ja-Young
authors.initials: JY
authors.lastname: Jang
authors.affiliation: Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
authors.forename: Jung-Sub
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.affiliation: Division of Applied Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Jin-Cheol
authors.initials: JC
authors.lastname: Kim
authors.affiliation: National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Republic of Korea.
authors.forename: Hyeong Hwan
authors.initials: HH
authors.lastname: Kim
authors.affiliation: World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Hae Woong
authors.initials: HW
authors.lastname: Park
mesh_terms: D001431:Bacteriological Techniques; D061249:Batch Cell Culture Techniques / Q000379:methods; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D025301:Hyphae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen; D010865:Pilot Projects; D011684:Purine Nucleosides / Q000096:biosynthesis*; D012399:Rotation; D013025:Glycine max / Q000737:chemistry; D013302:Streptomyces / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D003313:Zea mays / Q000737:chemistry
chemical_list: D003470:Culture Media; D011684:Purine Nucleosides; D002244:Carbon; C013551:herbicidins; D005947:Glucose; D009584:Nitrogen; D010100:Oxygen
keywords: Streptomyces scopuliridis M40; herbicidin A; hyphal length; oxygen uptake rate; scale-up