title: Enhancing Effects of Curcumin on Cytotoxicity of Paclitaxel, Methotrexate and Vincristine in Gastric Cancer Cells
abstract: Curcumin (Diferuloylmethane), a polyphenolic compound with antioxidant, anti-inflammatory and anticancer
  properties, has been found to increase chemotherapeutic agents-induced cytotoxicity in some resistant cancer cell
  lines. This investigation aimed to study the effects of curcumin on efficacy of some common anticancer agents in
  gastric cancer cells. AGS cells were cultured in RPMI-1640 medium under standard culture conditions (5% CO2 and
  95% humidified air at 37°C). Curcumin was used at concentrations of 5, 15, 30 and 50 μM. Cells were treated with a
  combination of curcumin and paclitaxel (300 nm) or methotrexate (100 μm) or vincristine (5 nm). Cell viability, the
  percentage of live cells in the whole population, was evaluated by MTT assay after 48 hours. The results showed that
  cell viability was significantly decreased after incubation of AGS cells with curcumin. Combination with curcumin
  (15-50 μm) significantly increased cytotoxicity of all three agents (P<0.001). Regarding high anticancer potential and
  enhancement of chemotherapeutic agent-induced cytotoxicity, the combined use of curcumin with standard chemotherapy
  of gastric cancer is suggested as a strategy for better management of this fatal cancer.
authors.affiliation: Department of Toxicology, 5Department of Chemical Engineering, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza. Isfahan. Email: Ebrahimifar67@gmail.com
authors.forename: Meysam
authors.initials: M
authors.lastname: Ebrahimifar
authors.forename: Majid
authors.initials: M
authors.lastname: Hasanzadegan Roudsari
authors.forename: S Maryam
authors.initials: SM
authors.lastname: Kazemi
authors.forename: Hasan
authors.initials: H
authors.lastname: Ebrahimi Shahmabadi
authors.forename: Leila
authors.initials: L
authors.lastname: Kanaani
authors.forename: S A
authors.initials: SA
authors.lastname: Alavi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Izadi Vasfi
keywords: Curcumin; gastric cancer; chemotherapy