title: Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by BacteriumBacillus subtilisA1.
abstract: In this work, the biodegradation of the crude oil by the potential biosurfactant producingBacillus subtilisA1 was investigated. The isolate had the ability to synthesize degradative enzymes such as alkane hydroxylase and alcohol dehydrogenase at the time of biodegradation of hydrocarbon. The biosurfactant producing conditions were optimized as pH 7.0, temperature 40°C, 2% sucrose and 3% of yeast extract as best carbon and nitrogen sources for maximum production of biosurfactant (4.85 g l-1). Specifically, the low molecular weight compounds, i.e., C10-C14were completely degraded, while C15-C19were degraded up to 97% from the total hydrocarbon pools. Overall crude oil degradation efficiency of the strain A1 was about 87% within a short period of time (7 days). The accumulated biosurfactant from the biodegradation medium was characterized to be lipopeptide in nature. The strain A1 was found to be more robust than other reported biosurfactant producing bacteria in degradation efficiency of crude oil due to their enzyme production capability and therefore can be used to remove the hydrocarbon pollutants from contaminated environment.
authors.affiliation: Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University Vellore, India.
authors.forename: Punniyakotti
authors.initials: P
authors.lastname: Parthipan
authors.affiliation: School of Aquatic Food Products and Technology, Kerala University of Fisheries and Ocean Studies Kochi, India.
authors.forename: Elumalai
authors.initials: E
authors.lastname: Preetham
authors.affiliation: Curtin Corrosion Engineering Industry Centre, School of Chemical and Petroleum Engineering, Curtin University, Bentley WA, Australia.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Machuca
authors.affiliation: School of Science and Engineering, Technology Futures Institute, Teesside UniversityMiddlesbrough, UK; TeeGene Biotech Ltd., Wilton CentreRedcar, UK.
authors.forename: Pattanathu K S M
authors.initials: PK
authors.lastname: Rahman
authors.affiliation: Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar UniversityCoimbatore, India; Thiruvalluvar UniversityVellore, India.
authors.forename: Kadarkarai
authors.initials: K
authors.lastname: Murugan
authors.affiliation: Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University Vellore, India.
authors.forename: Aruliah
authors.initials: A
authors.lastname: Rajasekar
keywords: Bacillus subtilis; biodegradation; biosurfactant; lipopeptide; petroleum remediation