title: DNApod: DNA polymorphism annotation database from next-generation sequence read archives.
abstract: With the rapid advances in next-generation sequencing (NGS), datasets for DNA polymorphisms among various species and strains have been produced, stored, and distributed. However, reliability varies among these datasets because the experimental and analytical conditions used differ among assays. Furthermore, such datasets have been frequently distributed from the websites of individual sequencing projects. It is desirable to integrate DNA polymorphism data into one database featuring uniform quality control that is distributed from a single platform at a single place. DNA polymorphism annotation database (DNApod; http://tga.nig.ac.jp/dnapod/) is an integrated database that stores genome-wide DNA polymorphism datasets acquired under uniform analytical conditions, and this includes uniformity in the quality of the raw data, the reference genome version, and evaluation algorithms. DNApod genotypic data are re-analyzed whole-genome shotgun datasets extracted from sequence read archives, and DNApod distributes genome-wide DNA polymorphism datasets and known-gene annotations for each DNA polymorphism. This new database was developed for storing genome-wide DNA polymorphism datasets of plants, with crops being the first priority. Here, we describe our analyzed data for 679, 404, and 66 strains of rice, maize, and sorghum, respectively. The analytical methods are available as a DNApod workflow in an NGS annotation system of the DNA Data Bank of Japan and a virtual machine image. Furthermore, DNApod provides tables of links of identifiers between DNApod genotypic data and public phenotypic data. To advance the sharing of organism knowledge, DNApod offers basic and ubiquitous functions for multiple alignment and phylogenetic tree construction by using orthologous gene information.
authors.affiliation: Genome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Mochizuki
authors.affiliation: Genome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tanizawa
authors.affiliation: Genome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Takatomo
authors.initials: T
authors.lastname: Fujisawa
authors.affiliation: Database Center for Life Science, Joint Support-Center for Data Science Research, Research Organization of Information and Systems, Mishima, Shizuoka, Japan.
authors.forename: Tazro
authors.initials: T
authors.lastname: Ohta
authors.affiliation: Department of Liberal Arts, The Open University of Japan, Chiba, Chiba, Japan.
authors.forename: Naruo
authors.initials: N
authors.lastname: Nikoh
authors.affiliation: Division of Citrus Research, Institute of Fruit Tree and Tea Science, NARO, Shimizu, Shizuoka, Japan.
authors.forename: Tokurou
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Comparative Genomics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.|Advanced Genomics Center, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.affiliation: Advanced Genomics Center, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Asao
authors.initials: A
authors.lastname: Fujiyama
authors.affiliation: Plant Genetics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Nori
authors.initials: N
authors.lastname: Kurata
authors.affiliation: Genome Informatics Group, Department of Technology Development, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nagasaki
authors.affiliation: Genome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Eli
authors.initials: E
authors.lastname: Kaminuma
authors.affiliation: Genome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D018556:Crops, Agricultural / Q000235:genetics; D004247:DNA / Q000235:genetics*; D018744:DNA, Plant; D030561:Databases, Nucleic Acid; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006720:Homozygote; D058977:Molecular Sequence Annotation; D012275:Oryza / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012016:Reference Values; D015203:Reproducibility of Results; D012984:Software; D045868:Sorghum / Q000235:genetics; D003313:Zea mays / Q000235:genetics
chemical_list: D018744:DNA, Plant; D004247:DNA