title: Macromolecular and Elemental Composition Analyses ofLeuconostoc mesenteroidesATCC 8293 Cultured in a Chemostat.
abstract: The cellular composition and metabolic compounds ofLeuconostoc mesenteroidesATCC 8293 were analyzed after cultivation in an anaerobic chemostat. The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA. Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine. Elements were in the order of C > O > N > H > S. The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM). These data provide a first view of the cellular composition ofL. mesenteroidesfor use in metabolic flux analysis.
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Jeongsu
authors.initials: J
authors.lastname: Bang
authors.affiliation: Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Hyunbin
authors.initials: H
authors.lastname: Seong
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Ye Won
authors.initials: YW
authors.lastname: Kwon
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Dong-Yup
authors.initials: DY
authors.lastname: Lee
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D018533:Biomass; D004269:DNA, Bacterial / Q000032:analysis; D004602:Elements; D005285:Fermentation; D005516:Food Microbiology; D005632:Fructose / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis; D000070007:Leuconostoc mesenteroides / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D008353:Mannitol / Q000032:analysis; D064688:Metabolic Flux Analysis; D011134:Polysaccharides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012329:RNA, Bacterial / Q000032:analysis
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; D004602:Elements; D008055:Lipids; D011134:Polysaccharides; D011506:Proteins; D012329:RNA, Bacterial; D005632:Fructose; D019344:Lactic Acid; D008353:Mannitol; D019342:Acetic Acid
keywords: Leuconostoc mesenteroides; composition; elements; macromolecules; metabolites