title: Identification of Biomarkers for Resistance toFusarium oxysporumf. sp.cubenseInfection andin SilicoStudies inMusa paradisiacaCultivar Puttabale through Proteomic Approach.
abstract: Panama wilt caused byFusarium oxysporumf. sp.cubense(Foc) is one of the major disease constraints of banana production. Previously, we reported the disease resistanceMusa paradisiaca cv.puttabale clones developed from Ethylmethanesulfonate and Foc culture filtrate against Foc inoculation. Here, the same resistant clones and susceptible clones were used for the study of protein accumulation against Foc inoculation by two-dimensional gel electrophoresis (2-DE), their expression pattern and anin silicoapproach. The present investigation revealed mass-spectrometry identified 16 proteins that were over accumulated and 5 proteins that were under accumulated as compared to the control. The polyphosphoinositide binding protein ssh2p (PBPssh2p) and Indoleacetic acid-induced-like (IAA) protein showed significant up-regulation and down-regulation. The docking of the pathogenesis-related protein (PR) with the fungal protein endopolygalacturonase (PG) exemplify the three ionic interactions and seven hydrophobic residues that tends to good interaction at the active site of PG with free energy of assembly dissociation (1.5 kcal/mol). The protein-ligand docking of the Peptide methionine sulfoxide reductase chloroplastic-like protein (PMSRc) with the ligand β-1,3 glucan showed minimum binding energy (-6.48 kcal/mol) and docking energy (-8.2 kcal/mol) with an interaction of nine amino-acid residues. These explorations accelerate the research in designing the host pathogen interaction studies for the better management of diseases.
authors.affiliation: Department of Post-Graduate Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577 451, India. venka.biotech@gmail.com.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Ramu
authors.affiliation: Department of Post-Graduate Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577 451, India. krishnabiotech2003@gmail.com.
authors.forename: Krishna
authors.initials: K
authors.lastname: Venkatarangaiah
authors.affiliation: Department of Biotechnology Engineering, M S Ramaiah Institute of Technology, Bangalore 560 054, Karnataka, India. pradie.k@gmail.com.
authors.forename: Pradeepa
authors.initials: P
authors.lastname: Krishnappa
authors.affiliation: Department of Post-Graduate Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577 451, India. santosh09.kumarsr@gmail.com.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Shimoga Rajanna
authors.affiliation: Department of Post-Graduate Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577 451, India. nagraja.dk@gmail.com.
authors.forename: Nagaraja
authors.initials: N
authors.lastname: Deeplanaik
authors.affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, India. chandrapalanup@gmail.com.
authors.forename: Anup
authors.initials: A
authors.lastname: Chandra Pal
authors.affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, India. krk@appbot.uni-mysore.ac.in.
authors.forename: Kukkundoor Ramachandra
authors.initials: KR
authors.lastname: Kini
keywords: Musa paradisiaca cv. puttabale; PR proteins; panama wilt; protein-protein docking; two dimensional gel electrophoresis