title: Genome-wide siRNA screen of genes regulating the LPS-induced NF-κB and TNF-α responses in mouse macrophages.
abstract: The mammalian innate immune system senses many bacterial stimuli through the toll-like receptor (TLR) family. Activation of the TLR4 receptor by bacterial lipopolysaccharide (LPS) is the most widely studied TLR pathway due to its central role in host responses to gram-negative bacterial infection and its contribution to endotoxemia and sepsis. Here we describe a genome-wide siRNA screen to identify genes regulating the mouse macrophage TNF-α and NF-κB responses to LPS. We include a secondary validation screen conducted with six independent siRNAs per gene to facilitate removal of off-target screen hits. We also provide microarray data from the same LPS-treated macrophage cells to facilitate downstream data analysis. These data provide a resource for analyzing gene function in the predominant pathway driving inflammatory signaling and cytokine expression in mouse macrophages.
authors.affiliation: Signaling Systems Unit, Laboratory of Systems Biology, Bethesda, Maryland 20892, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Signaling Systems Unit, Laboratory of Systems Biology, Bethesda, Maryland 20892, USA.|Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK.
authors.forename: Samuel
authors.initials: S
authors.lastname: Katz
authors.affiliation: Bioinformatics Team, Laboratory of Systems Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Dutta
authors.affiliation: Signaling Systems Unit, Laboratory of Systems Biology, Bethesda, Maryland 20892, USA.
authors.forename: Zachary L
authors.initials: ZL
authors.lastname: Benet
authors.affiliation: Signaling Systems Unit, Laboratory of Systems Biology, Bethesda, Maryland 20892, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Signaling Systems Unit, Laboratory of Systems Biology, Bethesda, Maryland 20892, USA.
authors.forename: Iain D C
authors.initials: ID
authors.lastname: Fraser
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha