title: Immunogenic potential of Rhipicephalus (Boophilus) microplus aquaporin 1 against Rhipicephalus sanguineus in domestic dogs.
abstract: This study evaluated a recombinant aquaporin 1 protein of Rhipicephalus (Boophilus) microplus (RmAQP1) as antigen in a vaccine against R. sanguineus. Five dogs were immunized with RmAQP1 (10 µg) + adjuvant (Montanide) (G1), and five were inoculated with adjuvant only (G2), three times. Twenty-one days after the last immunization, animals of both groups were challenged with R. sanguineus larvae, nymphs and adults, and their biotic potential was compared. Blood samples were collected before each immunization and every 28 days after the last immunization for 10 weeks. Serum antibody titers (IgG) were assessed by ELISA. We observed that: engorgement period of adult females from G1 was 12% shorter than G2; larvae from G1 had 8.7% longer engorgement period than G2 and weighed 7.2% less; nymphs from G1 had 4.5% shorter engorgement period than G2 and weighed 3.6% less; although the antibody titers increased following the second immunization, they rapidly decreased after the third immunization. Results indicated low immunoprotection of RmAQP1 against adult R. sanguineus ticks, and possible efficacy on larvae and nymphs fed on immunized dogs. Further studies should be performed for a full evaluation of the immunoprotection of RmAQP1 against R. sanguineus infestations in dogs.
authors.affiliation: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil.
authors.forename: Patricia Martinez
authors.initials: PM
authors.lastname: Évora
authors.affiliation: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil.
authors.forename: Gustavo Seron
authors.initials: GS
authors.lastname: Sanches
authors.affiliation: Knipling-Bushland U.S. Livestock Insects Research Laboratory, Veterinary Pest Genomics Center, Agricultural Research Service - ARS, United States Department of Agriculture - USDA, Kerrville, Texas, USA.
authors.forename: Felix David
authors.initials: FD
authors.lastname: Guerrero
authors.affiliation: Knipling-Bushland U.S. Livestock Insects Research Laboratory, Veterinary Pest Genomics Center, Agricultural Research Service - ARS, United States Department of Agriculture - USDA, Kerrville, Texas, USA.
authors.forename: Adalberto Pérez de
authors.initials: AP
authors.lastname: León
authors.affiliation: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil.|Programa de Pós-graduação em Ciência Animal, Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná - PUCPR, Curitiba, PR, Brasil.
authors.forename: Gervásio Henrique
authors.initials: GH
authors.lastname: Bechara
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control*; D004285:Dogs; D005260:Female; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D048494:Rhipicephalus / Q000276:immunology*; D048508:Rhipicephalus sanguineus / Q000276:immunology*; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D051398:Aquaporin 1