title: Beneficial effects of fermented sardinelle protein hydrolysates on hypercaloric diet induced hyperglycemia, oxidative stress and deterioration of kidney function in wistar rats.
abstract: This study investigated the potential effects of fermented sardinelle protein hydrolysates (FSPHs) obtained by two proteolytic bacteria,Bacillus subtilisA26 (FSPH-A26) andBacillus amyloliquefaciensAn6 (FSPH-An6), on hypercaloric diet (HCD) induced hyperglycemia and oxidative stress in rats. Effects of FSPHs on blood glucose level, glucose tolerance, α-amylase activity and hepatic glycogen content were investigated, as well as their effect on the oxidative stress state. Biochemical findings revealed that, while undigested sardinelle proteins did not exhibit hypoglycemic activity, oral administration of FSPHs to HCD-fed rats reduced significantly α-amylase activity as well as glycemia and hepatic glycogen levels. Further, the treatment with FSPHs improved the redox status by decreasing the levels of lipid peroxidation products and increasing the activities of the antioxidant enzymes (superoxide dismutase, glutathione peroxidase and catalase) and the level of glutathione in the liver and kidneys, as compared to those of HCD-fed rats. FSPHs were also found to exert significant protective effects on liver and kidney functions, evidenced by a marked decrease in alkaline phosphatase activity and a modulation of creatinine and uric acid contents. These results indicated the beneficial effect of FSPHs on the prevention from hyperglycemia and oxidative stress.
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P. O. Box 1173, 3038 Sfax, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Jemil
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P. O. Box 1173, 3038 Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Nasri
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P. O. Box 1173, 3038 Sfax, Tunisia.
authors.forename: Ola
authors.initials: O
authors.lastname: Abdelhedi
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, Paterna, 46980 Valencia, Spain.
authors.forename: Maria-Concepción
authors.initials: MC
authors.lastname: Aristoy
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P. O. Box 1173, 3038 Sfax, Tunisia.
authors.forename: Rabeb Ben Slama-Ben
authors.initials: RBS
authors.lastname: Salem
authors.affiliation: Laboratory of Hematology, Habib Bourguiba Hospital, 3029 Sfax, Tunisia.
authors.forename: Choumous
authors.initials: C
authors.lastname: Kallel
authors.affiliation: Laboratory of Biochemistry, CHU Hedi Chaker, 3029 Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Marrekchi
authors.affiliation: Laboratory of Biochemistry, CHU Hedi Chaker, 3029 Sfax, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Jamoussi
authors.affiliation: Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax (FSS), University of Sfax, P. O. Box 95, 3052 Sfax, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: ElFeki
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P. O. Box 1173, 3038 Sfax, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hajji
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, Paterna, 46980 Valencia, Spain.
authors.forename: Fidel
authors.initials: F
authors.lastname: Toldrá
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P. O. Box 1173, 3038 Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
keywords: Antioxidant; Fermented sardinelle protein hydrolysates; Hepato-protective; Hypoglycemic; Reno-protective