title: [Recombinant human bone morphogenetic protein 7 inhibits the proliferation and extracellular matrix secretion of fibroblast of urethral scars].
abstract: Objective:To study the effect and its mechanism of recombinant human bone morphogenetic protein 7 (rhBMP-7) on urethral keloid fibroblast.Methods:Urethral fibroblasts were extracted and cultured, and the fourth-generation fibroblasts were used for experiment. Urethral fibroblasts were treated with rhBMP-7. Proliferation rate was detected by CCK-8 method. RT-PCR was applied to test changes of mRNA in α smooth muscle actin (α-SMA) and type Ⅰ collagen protein (COL-1) of each group. Western blot was used to measure α-SMA protein expression and the effect of rhBMP-7 on signaling pathway of urethral keloid fibroblasts.Results:With the increase of rhBMP-7 concentrations, the proliferation rate of urethral keloid fibroblast was generally decreased. Cells kept proliferating with time at the same concentration of rhBMP-7. mRNA level of COL-1 and α-SMA in rhBMP-7-treated (>20 ng/ml) urethral keloid fibroblasts was greatly reduced with statistical significance (P<0.05) and dose dependency. It was found rhBMP-7 inhibited the overexpression of p-smad2/3 and α-SMA mediated by transforming growth factor beta 1 (TGF-β(1)) signaling pathway.Conclusion:rhBMP-7 could adjust TGF-β(1) signaling pathway to inhibit gene expression of COL-1 and a-SMA in urethral keloid fibroblast, which provided evidences for further animal experiments or clinical trials.
authors.affiliation: Department of Urology, Affiliated Hospital of Nantong University, Nantong 226001, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: L M
authors.initials: LM
authors.lastname: Ma
authors.forename: B
authors.initials: B
authors.lastname: Cai
authors.forename: H L
authors.initials: HL
authors.lastname: Li
authors.forename: Q W
authors.initials: QW
authors.lastname: Xing
mesh_terms: D000199:Actins; D055419:Bone Morphogenetic Protein 7; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I; D005109:Extracellular Matrix; D005347:Fibroblasts; D006801:Humans; D007627:Keloid; D012333:RNA, Messenger; D015398:Signal Transduction; D051899:Smad2 Protein; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014521:Urethra / Q000293:injuries*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D024042:Collagen Type I; D012333:RNA, Messenger; C497782:SMAD2 protein, human; D051899:Smad2 Protein; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1
keywords: Bone Morphogenetic Protein 7; Fibroblasts; Keloid; Transforming Growth Factor beta1