title: Assessment of MiRNA Regulation of Endothelial Progenitor Cell Mediated Angiogenesis.
abstract: Organ outgrowth, embryonic development, wound healing, and many such processes require the process of angiogenesis, whereby new blood vessels are developed from the preexisting vessels. microRNAs (miRs) are 18-24 nucleotide-containing endogenous RNAs that, via a posttranscriptional mechanism, exert substantial gene regulatory effects. It was discovered by recent advances that, through direct targeting of certain critical secretory factors and transcription factors, miRs exert potent angiogenic control in a cell autonomous and non-cell autonomous manner. This chapter comprehensively summarizes step-by-step protocols for the (1) transfection of miRNA in EPCs (2) advantages and limitations of the principal tubule formation assays in use.
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, 1675 University Blvd., Volker Hall G094, Birmingham, AL, 35294, USA.
authors.forename: Darukeshwara
authors.initials: D
authors.lastname: Joladarashi
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, 1675 University Blvd., Volker Hall G094, Birmingham, AL, 35294, USA. prasanak@uab.edu.
authors.forename: Prasanna
authors.initials: P
authors.lastname: Krishnamurthy
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D057054:Molecular Imaging / Q000379:methods; D018919:Neovascularization, Physiologic / Q000235:genetics*; D059467:Transcriptome; D014162:Transfection; D057188:Workflow
chemical_list: D017077:Culture Media, Conditioned; D035683:MicroRNAs
keywords: Angiogenesis; Endothelial progenitor cells; Matrigel; MiRNA; Tubule formation