title: AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp.
abstract: Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This study is the first report on QQ activity of bacteria isolated from the polar regions.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science University of Malaya, 50603 Kuala Lumpur, Malaysia.|National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wah Seng
authors.initials: WS
authors.lastname: See-Too
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Robson
authors.initials: R
authors.lastname: Ee
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yan-Lue
authors.initials: YL
authors.lastname: Lim
authors.affiliation: National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.|British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Convey
authors.affiliation: National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.|British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, UK.|Faculty of Health and Life Sciences, University of Northumbria, Newcastle Upon Tyne NE1 8ST, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Pearce
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science University of Malaya, 50603 Kuala Lumpur, Malaysia.|UM Omics Centre, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics*; D002265:Carboxylic Ester Hydrolases / Q000145:classification / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D010802:Phylogeny; D058642:Planococcus Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D053038:Quorum Sensing; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; C088386:homoserine lactone; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; D015107:4-Butyrolactone