title: Dendritic Cells Targeting and pH-Responsive Multi-layered Nanocomplexes for Smart Delivery of DNA Vaccines.
abstract: Specific and effective delivery of DNA vaccines into dendritic cells (DCs) to express antigens is a precondition for induction of immune responses. Construction of a new DNA vaccine delivery system with the ability of programmed gene transfection may achieve this objective. In this study, we successfully integrated dendritic lipopeptide, charge-reversible polymer, and APC-targeted material into DNA vaccine delivery system through layer-by-layer (LBL) assembly. By the means of adjusting the weight ratios and concentration ratios of components, stable complexes were formulated with a particle size of 256.8 ± 10.7 nm and zeta potential of 25.1 ± 2.3 mV. Moreover, this DNA vaccine delivery system could achieve specific delivery into DCs, high transfection efficiency and low cytotoxicity, holding great promise for immunotherapy.
authors.affiliation: Institute of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China.
authors.forename: Menglu
authors.initials: M
authors.lastname: Xu
authors.affiliation: Institute of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China.
authors.forename: Parikshit
authors.initials: P
authors.lastname: Banerjee
authors.affiliation: Institute of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China. zongcpu@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zong
authors.affiliation: Institute of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China. jianglei0453@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
mesh_terms: D003713:Dendritic Cells / Q000276:immunology*; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage*
chemical_list: D019444:Vaccines, DNA
keywords: DNA vaccine delivery system; charge-reversible; dendritic cells; layer-by-layer assembly; virus-inspired nanovectors