title: Development and application of a T7 RNA polymerase-dependent expression system for antibiotic production improvement in Streptomyces.
abstract: OBJECTIVE
  To develop a reliable and easy to use expression system for antibiotic production improvement of Streptomyces.
  
  
  RESULTS
  A two-compound T7 RNA polymerase-dependent gene expression system was developed to fulfill this demand. In this system, the T7 RNA polymerase coding sequence was optimized based on the codon usage of Streptomyces coelicolor. To evaluate the functionality of this system, we constructed an activator gene overexpression strain for enhancement of actinorhodin production. By overexpression of the positive regulator actII-ORF4 with this system, the maximum actinorhodin yield of engineered strain was 15-fold higher and the fermentation time was decreased by 48 h.
  
  
  CONCLUSIONS
  The modified two-compound T7 expression system improves both antibiotic production and accelerates the fermentation process in Streptomyces. This provides a general and useful strategy for strain improvement of important antibiotic producing Streptomyces strains.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.|School of Life Science, Southwest University, Chongqing, 400715, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.|School of Life Science, Southwest University, Chongqing, 400715, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.|School of Life Science, Southwest University, Chongqing, 400715, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.|School of Life Science, Southwest University, Chongqing, 400715, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.|School of Life Science, Southwest University, Chongqing, 400715, China.
authors.forename: Jialing
authors.initials: J
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China. zyzhou@swu.edu.cn.|School of Life Science, Southwest University, Chongqing, 400715, China. zyzhou@swu.edu.cn.|Colleges of Life Science, Chongqing Normal University, Chongqing, 400047, China. zyzhou@swu.edu.cn.
authors.forename: Zeyang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000880:Anthraquinones / Q000032:analysis / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism*; D001709:Biotechnology; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D012321:DNA-Directed RNA Polymerases; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; C013390:actinorhodin; D000095622:Benzoisochromanequinones
keywords: Antibiotics; Production improvement; Streptomyces; T7 RNA polymerase