title: Age-Related Loss in Bone Mineral Density of Rats Fed Lifelong on a Fish Oil-Based Diet Is Avoided by Coenzyme Q10Addition.
abstract: During aging, bone mass declines increasing osteoporosis and fracture risks. Oxidative stress has been related to this bone loss, making dietary compounds with antioxidant properties a promising weapon. Male Wistar rats were maintained for 6 or 24 months on diets with fish oil as unique fat source, supplemented or not with coenzyme Q10(CoQ10), to evaluate the potential of adding this molecule to then-3 polyunsaturated fatty acid (n-3 PUFA)-based diet for bone mineral density (BMD) preservation. BMD was evaluated in the femur. Serum osteocalcin, osteopontin, receptor activator of nuclear factor-κB ligand, ostroprotegerin, parathyroid hormone, urinary F₂-isoprostanes, and lymphocytes DNA strand breaks were also measured. BMD was lower in aged rats fed a diet without CoQ10respect than their younger counterparts, whereas older animals receiving CoQ10showed the highest BMD. F₂-isoprostanes and DNA strand breaks showed that oxidative stress was higher during aging. Supplementation with CoQ10prevented oxidative damage to lipid and DNA, in young and old animals, respectively. Reduced oxidative stress associated to CoQ10supplementation of thisn-3 PUFA-rich diet might explain the higher BMD found in aged rats in this group of animals.
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. alvarela@ugr.es.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Varela-López
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. jjochoa@ugr.es.
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Ochoa
authors.affiliation: Nuclear Medicine Service, Hospital Virgen de las Nieves, 18014 Granada, Spain. josem.llamas.sspa@juntadeandalucia.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Llamas-Elvira
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. maglopez@ugr.es.
authors.forename: Magdalena
authors.initials: M
authors.lastname: López-Frías
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. elenamp@ugr.es.
authors.forename: Elena
authors.initials: E
authors.lastname: Planells
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Biochemistry and Molecular Biology II, Biomedical Research Center, University of Granada, 18100 Granada, Spain. mramirez@ugr.es.
authors.forename: MCarmen
authors.initials: M
authors.lastname: Ramirez-Tortosa
authors.affiliation: Department of Pathology, Complejo Hospitalario de Jaén, 23007 Jaén, Spain. cesarl.ramirez.sspa@juntadeandalucia.es.
authors.forename: Cesar L
authors.initials: CL
authors.lastname: Ramirez-Tortosa
authors.affiliation: Department of Scienze Cliniche Specialistiche ed Odontostomatologiche, Università Politecnica delle Marche, 60131 Ancona, Italy. fgiampie@mta01.univpm.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
authors.affiliation: Department of Scienze Cliniche Specialistiche ed Odontostomatologiche, Università Politecnica delle Marche, 60131 Ancona, Italy. m.a.battino@univpm.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. jlquiles@ugr.es.
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D004032:Diet; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005269:Femur; D005395:Fish Oils / Q000008:administration & dosage*; D015227:Lipid Peroxidation; D008297:Male; D010024:Osteoporosis / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives*
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D014451:Ubiquinone; C024989:coenzyme Q10
keywords: antioxidants; dietary fat; n-3 PUFA; oxidative stress; ubiquinone