title: Synthesis, hybridization and fluorescence properties of a 2'-C-pyrene-triazole modified arabino-uridine nucleotide.
abstract: A new pyrene-modified nucleotide monomer is introduced, wherein pyrene is attached to the 2'-position of arabino-uridine through a triazolemethyl linker. When the monomer is introduced into oligonucleotides, very stable DNA duplexes and three way junctions are obtained. An oligonucleotide featuring two modifications in the center shows four-fold increase in the intensity of the pyrene excimer signal on hybridization with an RNA target but not with a DNA target. The new nucleotide monomer has potential in DNA invader probes as well as in RNA targeting and detection.
authors.affiliation: Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, 5230 Odense M, Denmark.
authors.forename: Pawan
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, Kurukshetra University, Kurukshetra 136 119, India.
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Sharma
authors.affiliation: Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, 5230 Odense M, Denmark. Electronic address: pouln@sdu.dk.
authors.forename: Poul
authors.initials: P
authors.lastname: Nielsen
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D005453:Fluorescence; D009711:Nucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes; D066244:Proton Magnetic Resonance Spectroscopy; D011721:Pyrenes / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014230:Triazoles / Q000737:chemistry*
chemical_list: D009711:Nucleotides; D015345:Oligonucleotide Probes; D011721:Pyrenes; D014230:Triazoles; C030984:pyrene
keywords: Click chemistry; Fluorescent probes; Pyrene; RNA targeting; Three way junctions