title: Genotyping Leishmania promastigotes isolated from patients with cutaneous leishmaniasis in south-eastern Turkey.
abstract: Objective Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods. Methods Novy-MacNeal-Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal transcribed spacer 1 (ITS1) of the Leishmania genome in cultures containing Leishmania promastigotes from 100 patients, which were genotyped with a restriction fragment length polymorphism (RFLP) analysis. A phylogenetic tree was constructed from ITS1 sequences of 95 culture fluid samples from these patients. Results Leishmania amastigotes were detected in 92% of cultures with growth. Leishmania promastigotes were typed as Leishmania tropica with both PCR-RFLP and sequencing. Conclusions Identification of L. tropica as the causative agent of CL in our region allows the clinical course to be predicted, and guides treatment decisions and preventive measures.
authors.affiliation: 1 Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey.
authors.forename: Fahriye
authors.initials: F
authors.lastname: Ekşi
authors.affiliation: 2 Department of Skin and Venereal Diseases, Gaziantep University, Gaziantep, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Özgöztaşı
authors.affiliation: 1 Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey.
authors.forename: Tekin
authors.initials: T
authors.lastname: Karslıgil
authors.affiliation: 1 Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sağlam
mesh_terms: D000293:Adolescent; D000328:Adult; D001399:Azure Stains; D021901:DNA, Intergenic / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007895:Leishmania tropica / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology; D008018:Life Cycle Stages / Q000235:genetics*; D008297:Male; D058889:Molecular Typing; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012036:Refugees; D017422:Sequence Analysis, DNA; D012867:Skin / Q000469:parasitology / Q000473:pathology; D014421:Turkey / Q000453:epidemiology
chemical_list: D001399:Azure Stains; D021901:DNA, Intergenic; D016054:DNA, Protozoan
keywords: Cutaneous leishmaniasis; PCR–RFLP; culture; phylogenetic tree; sequence analysis