title: Adhesion of ligand-conjugated biosynthesized magnetite nanoparticles to triple negative breast cancer cells.
abstract: This paper presents the results of an experimental study of the adhesion forces between components of model conjugated magnetite nanoparticle systems for improved selectivity in the specific targeting of triple negative breast cancer. Adhesion forces between chemically synthesized magnetite nanoparticles (CMNPs), biosynthesized magnetite nanoparticles (BMNPs), as well as their conjugated systems and triple negative breast cancer cells (MDA-MB-231) or normal breast cells (MCF 10A) are elucidated at a nanoscale. In all cases, the BMNPs had higher adhesion forces (to breast cancer cells and normal breast cells) than CMNPs. The adhesion of LHRH-conjugated BMNPs or BSA-conjugated BMNPs to cancer cells is shown to be about 6 times to that of normal breast cells. The increase in adhesion forces between luteinizing hormone-releasing hormone, LHRH- or EphA2, a breast specific antibody(BSA)-conjugated BMNPs to breast cancer cells is attributed to van der Waals interactions between the peptides/antibodies from the conjugated nanoparticles and the over-expressed receptors (revealed using immunofluorescence staining) on the surfaces of the breast cancer. The implications of the results are discussed for the selectivity and specificity of breast cancer targeting by ligand-conjugated BMNPs.
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, NJ 08544, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Obayemi
authors.affiliation: Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, NJ 08544, USA.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, NJ 08544, USA.
authors.forename: Vanessa O
authors.initials: VO
authors.lastname: Uzonwanne
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Sheda Science and Technology Complex (SHESTCO) Abuja, Federal Capital Territory, Nigeria.
authors.forename: Olushola S
authors.initials: OS
authors.lastname: Odusanya
authors.affiliation: Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, NJ 08544, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Malatesta
authors.affiliation: Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Department of Chemistry, Bronx Community College, New York, NY, USA.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Anuku
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, NJ 08544, USA; Materials Program, Department of Mechanical Engineering, Worcester Polytechnic Institute (WPI), 100 Institute Road, Worcester, MA 01609, USA; Department of Biomedical Engineering, Worcester Polytechnic Institute (WPI), Gateway Park, Life Sciences and Bioengineering Center, 60 Prescott Street, Worcester, MA 01605, USA. Electronic address: soboyejo@princeton.edu.
authors.forename: Winston O
authors.initials: WO
authors.lastname: Soboyejo
mesh_terms: D000268:Adhesiveness; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D058726:Nanoconjugates / Q000737:chemistry*; D036104:Receptor, EphA2 / Q000737:chemistry; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism*
chemical_list: D058185:Magnetite Nanoparticles; D058726:Nanoconjugates; D007987:Gonadotropin-Releasing Hormone; D036104:Receptor, EphA2
keywords: Biosynthesized magnetite nanoparticles (BMNPs); Breast specific antibody (BSA); Chemically synthesized magnetite nanoparticles (CMNPs); Ephrin type-A receptor 2 (EphA2); Luteinizing hormone-releasing hormone (LHRH)