title: Involvement of Cellular Prion Protein in α-Synuclein Transport in Neurons.
abstract: The cellular prion protein, encoded by the gene Prnp, has been reported to be a receptor of β-amyloid. Their interaction is mandatory for neurotoxic effects of β-amyloid oligomers. In this study, we aimed to explore whether the cellular prion protein participates in the spreading of α-synuclein. Results demonstrate that Prnp expression is not mandatory for α-synuclein spreading. However, although the pathological spreading of α-synuclein can take place in the absence of Prnp, α-synuclein expanded faster in PrPC-overexpressing mice. In addition, α-synuclein binds strongly on PrPC-expressing cells, suggesting a role in modulating the effect of α-synuclein fibrils.
authors.affiliation: Molecular and Cellular Neurobiotechnology, Institute of Bioengineering of Catalonia (IBEC), Parc Científic de Barcelona, Baldiri Reixac 15-21, E-08028, Barcelona, Spain.|Department of Cell Biology, Physiology and Immunology, Universitat de Barcelona, Barcelona, Spain.|Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain.|Institute of Neuroscience, University of Barcelona, Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Urrea
authors.affiliation: Molecular and Cellular Neurobiotechnology, Institute of Bioengineering of Catalonia (IBEC), Parc Científic de Barcelona, Baldiri Reixac 15-21, E-08028, Barcelona, Spain.|Department of Cell Biology, Physiology and Immunology, Universitat de Barcelona, Barcelona, Spain.|Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain.|Institute of Neuroscience, University of Barcelona, Barcelona, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Segura-Feliu
authors.affiliation: Department of Dementia and Higher Brain Function, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, 156-8506, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Masuda-Suzukake
authors.affiliation: Molecular and Cellular Neurobiotechnology, Institute of Bioengineering of Catalonia (IBEC), Parc Científic de Barcelona, Baldiri Reixac 15-21, E-08028, Barcelona, Spain.|Department of Cell Biology, Physiology and Immunology, Universitat de Barcelona, Barcelona, Spain.|Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain.|Institute of Neuroscience, University of Barcelona, Barcelona, Spain.
authors.forename: Arnau
authors.initials: A
authors.lastname: Hervera
authors.affiliation: Bacterial infections: antimicrobial therapies. Institute of Bioengineering of Catalonia (IBEC), Parc Científic de Barcelona, Barcelona, Spain.
authors.forename: Lucas
authors.initials: L
authors.lastname: Pedraz
authors.affiliation: Multiscale in Mechanical and Biological Engineering (M2BE), Aragon Institute of Engineering Research, Department of Mechanical Engineering, University of Zaragoza, Zaragoza, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: García Aznar
authors.affiliation: Neurodegenerative Diseases Research Group, Vall d'Hebron Research Institute-Center for Networked Biomedical Research on Neurodegenerative Diseases, Autonomous University of Barcelona, Barcelona, Spain.|Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, Spain.
authors.forename: Miquel
authors.initials: M
authors.lastname: Vila
authors.affiliation: Nanobioengineering Group, Institute for Bioengineering of Catalonia, (IBEC), Parc Científic de Barcelona, Barcelona, Spain.|Department of Electronics, University of Barcelona, Martí i Franquès 1, E-08028, Barcelona, Spain.|Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029, Madrid, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier
authors.affiliation: Bacterial infections: antimicrobial therapies. Institute of Bioengineering of Catalonia (IBEC), Parc Científic de Barcelona, Barcelona, Spain.
authors.forename: Eduard
authors.initials: E
authors.lastname: Torrents
authors.affiliation: Institute of Neuroscience, University of Barcelona, Barcelona, Spain.|Institut de Neuropatologia, IDIBELL-Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Spain.|Departamento de Patologia y Terapeutica Experimental, Facultad de Medicina, Universidad de Barcelona, Barcelona, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain.
authors.forename: Isidro
authors.initials: I
authors.lastname: Ferrer
authors.affiliation: Molecular and Cellular Neurobiotechnology, Institute of Bioengineering of Catalonia (IBEC), Parc Científic de Barcelona, Baldiri Reixac 15-21, E-08028, Barcelona, Spain.|Department of Cell Biology, Physiology and Immunology, Universitat de Barcelona, Barcelona, Spain.|Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain.|Institute of Neuroscience, University of Barcelona, Barcelona, Spain.
authors.forename: Rosalina
authors.initials: R
authors.lastname: Gavín
authors.affiliation: Department of Dementia and Higher Brain Function, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, 156-8506, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Hagesawa
authors.affiliation: Molecular and Cellular Neurobiotechnology, Institute of Bioengineering of Catalonia (IBEC), Parc Científic de Barcelona, Baldiri Reixac 15-21, E-08028, Barcelona, Spain. jadelrio@ibecbarcelona.eu.|Department of Cell Biology, Physiology and Immunology, Universitat de Barcelona, Barcelona, Spain. jadelrio@ibecbarcelona.eu.|Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain. jadelrio@ibecbarcelona.eu.|Institute of Neuroscience, University of Barcelona, Barcelona, Spain. jadelrio@ibecbarcelona.eu.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Del Río
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D018621:PrPC Proteins / Q000235:genetics* / Q000378:metabolism*; D000072002:Prion Proteins / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology; D051844:alpha-Synuclein / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018621:PrPC Proteins; D000072002:Prion Proteins; D051844:alpha-Synuclein
keywords: Amyloid spreading; Microfluidic devices; Prnp; Synuclein