title: Enhancement of Bone-Marrow-Derived Mesenchymal Stem Cell Angiogenic Capacity by NPWT for a Combinatorial Therapy to Promote Wound Healing with Large Defect.
abstract: Poor viability of engrafted bone marrow mesenchymal stem cells (BMSCs) often hinders their application for wound healing, and the strategy of how to take full advantage of their angiogenic capacity within wounds still remains unclear. Negative pressure wound therapy (NPWT) has been demonstrated to be effective for enhancing wound healing, especially for the promotion of angiogenesis within wounds. Here we utilized combinatory strategy using the transplantation of BMSCs and NPWT to investigate whether this combinatory therapy could accelerate angiogenesis in wounds. In vitro, after 9-day culture, BMSCs proliferation significantly increased in NPWT group. Furthermore, NPWT induced their differentiation into the angiogenic related cells, which are indispensable for wound angiogenesis. In vivo, rat full-thickness cutaneous wounds treated with BMSCs combined with NPWT exhibited better viability of the cells and enhanced angiogenesis and maturation of functional blood vessels than did local BMSC injection or NPWT alone. Expression of angiogenesis markers (NG2, VEGF, CD31, andα-SMA) was upregulated in wounds treated with combined BMSCs with NPWT. Our data suggest that NPWT may act as an inductive role to enhance BMSCs angiogenic capacity and this combinatorial therapy may serve as a simple but efficient clinical solution for complex wounds with large defects.
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Kangquan
authors.identifier: 0000-0001-7759-9902
authors.initials: K
authors.lastname: Shou
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Yahui
authors.identifier: 0000-0002-8360-9959
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Xun
authors.identifier: 0000-0003-0202-5837
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Chao
authors.identifier: 0000-0002-3624-5558
authors.initials: C
authors.lastname: Jian
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Baiwen
authors.identifier: 0000-0002-7671-2587
authors.initials: B
authors.lastname: Qi
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Xiang
authors.identifier: 0000-0002-1529-4561
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Aixi
authors.identifier: 0000-0002-3751-0894
authors.initials: A
authors.lastname: Yu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D003131:Combined Modality Therapy; D016207:Cytokines / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D054843:Negative-Pressure Wound Therapy; D018919:Neovascularization, Physiologic; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D016207:Cytokines