title: Ulipristal acetate administration at mid-cycle changes gene expression profiling of endometrial biopsies taken during the receptive period of the human menstrual cycle.
abstract: The aim of this study was to analyze the effects of mid-cycle administration of Ulipristal acetate (UPA) on gene expression in endometrial biopsies taken during the receptive phase of the cycle. Fourteen healthy menstruating women were studied during 14 control non-treated and 12 treated cycles with a single dose of 30 mg UPA when follicle diameter reached 20 mm. Ovulation in both treated and control cycles was confirmed by serial determinations of serum LH, progesterone and vaginal ultrasound. An endometrial biopsy at day LH+7, in each cycle, was taken for RNA microarray and qPCR analysis or prepared for histological and immunohistochemistry studies. Functional analysis of differentially expressed genes showed the presence of changes compatible with a non-receptive endometrial phenotype, further confirmed by qPCR and immunohistochemistry. This study suggests the effects of UPA on endometrial receptivity, offering a plausible explanation for the higher contraceptive efficacy of this method compared to that of levonorgestrel.
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México 14080, México.
authors.forename: Saúl
authors.initials: S
authors.lastname: Lira-Albarrán
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México 14080, México.
authors.forename: Marta
authors.initials: M
authors.lastname: Durand
authors.affiliation: Departamento de Genómica Computacional, Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Ciudad de México 14610, México.
authors.forename: Marco F
authors.initials: MF
authors.lastname: Larrea-Schiavon
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México 14080, México.
authors.forename: Leticia
authors.initials: L
authors.lastname: González
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México 14080, México.
authors.forename: David
authors.initials: D
authors.lastname: Barrera
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México 14080, México.
authors.forename: Claudia
authors.initials: C
authors.lastname: Vega
authors.affiliation: Departamento de Patología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México 14080, México.
authors.forename: Armando
authors.initials: A
authors.lastname: Gamboa-Domínguez
authors.affiliation: Departamento de Genómica Computacional, Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Ciudad de México 14610, México.
authors.forename: Claudia
authors.initials: C
authors.lastname: Rangel
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México 14080, México. Electronic address: fernando.larreag@incmnsz.mx.
authors.forename: Fernando
authors.initials: F
authors.lastname: Larrea
mesh_terms: D016000:Cluster Analysis; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D063990:Gene Ontology; D006801:Humans; D008597:Menstrual Cycle / Q000187:drug effects / Q000235:genetics*; D009649:Norpregnadienes / Q000008:administration & dosage* / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D009649:Norpregnadienes; C555622:ulipristal acetate
keywords: Contraception; Endometrium; Gene expression; Ulipristal acetate