title: Characterization and functional analysis of serpin-1 like gene from oak silkworm Antheraea pernyi.
abstract: Serpins are a broadly distributed family of proteases found in various organisms that play an important role in regulating the immune response. Here, we identified a serpin-1 gene from Antheraea pernyi that encodes a 279 amino acid protein with a molecular weight of 30.8 kDa. We expressed the recombinant Ap-serpin-1 protein in Escherichia coli and used the purified protein to prepare rabbit anti-Ap-serpin-1 polyclonal antibodies. We calculated the enzyme-linked immunosorbent assay titer of the antibody as 1:128000. Quantitative real-time polymerase chain reaction analysis revealed that Ap-serpin-1 was expressed in all examined tissues, including hemolymph, malpighian tubules, midgut, silk gland, integument and the fat body; the highest Ap-serpin-1 expression levels was detected in the fat body. We next investigated the expression patterns of Ap-serpin-1 in both fat body and hemolymph samples, following treatment with E. coli, Beauveria bassiana, Micrococcus luteus and nuclear polyhedrosis virus (NPV). We reported that NPV and M. luteus significantly enhanced Ap-serpin-1 expression in the fat body. While, in the hemolymph samples, treatment with B. bassiana and M. luteus was shown to upregulate Ap-serpin-1 expression at 24 h induction. Altogether, our results suggest that Ap-serpin-1 is involved in the innate immunity of A. pernyi.
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: H M
authors.initials: HM
authors.lastname: Yu
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: B J
authors.initials: BJ
authors.lastname: Zhu
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Wei
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: C
authors.initials: C
authors.lastname: Qian
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: M
authors.initials: M
authors.lastname: Nadeem Abbas
authors.affiliation: College of Life Sciences,Anhui Agricultural University,Hefei,230036,China.
authors.forename: C L
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000818:Animals; D005216:Fat Body / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008018:Life Cycle Stages; D008297:Male; D009036:Moths / Q000502:physiology*; D011817:Rabbits; D015843:Serpins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D015843:Serpins
keywords: 
                 Antheraea pernyi
              ; expression; innate immunity; serpin-1