title: Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-like Disease in NOD Mice.
abstract: Compact bone (cortical or dense bone) is among the organs that contain multipotent mesenchymal stromal cells (MSCs). Unlike bone marrow plugs where MSCs were initially isolated, compact bone has minimal (amount of) hematopoietic cells and thus facilitates the MSCs isolation process. In vitro, MSCs from compact bone show multipotency and differentiation into mesenchymal tissues such as bone, adipose, and cartilage, under certain conditions. MSCs therapy has been promising in preclinical and clinical studies against autoimmune diseases. Not only can MSCs replace the lost tissue through their regenerative properties, but they can also control the autoimmune attacks by immunoregulatory cytokines. This protocol describes the use of compact bone-derived MSCs to preserve salivary function (saliva flow/output) in the NOD (nonobese diabetic) mouse model affected with Sjogren's-like disease.
authors.affiliation: Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of Dentistry, McGill University, 3640 University Street, M43, Montreal, QC, Canada.|University of Jordan, Amman, Jordan.
authors.forename: Ghada Abu
authors.initials: GA
authors.lastname: Elghanam
authors.affiliation: Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of Dentistry, McGill University, 3640 University Street, M43, Montreal, QC, Canada.
authors.forename: Younan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: University of Toronto, Toronto, ON, Canada.
authors.forename: Saeed
authors.initials: S
authors.lastname: Khalili
authors.affiliation: Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of Dentistry, McGill University, 3640 University Street, M43, Montreal, QC, Canada.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Fang
authors.affiliation: Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of Dentistry, McGill University, 3640 University Street, M43, Montreal, QC, Canada. simon.tran@mcgill.ca.
authors.forename: Simon D
authors.initials: SD
authors.lastname: Tran
mesh_terms: D050156:Adipogenesis; D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D020219:Chondrogenesis; D003114:Colony-Forming Units Assay; D000071538:Cortical Bone / Q000166:cytology*; D004195:Disease Models, Animal; D016130:Immunophenotyping; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D010012:Osteogenesis; D012859:Sjogren's Syndrome / Q000209:etiology / Q000628:therapy*
chemical_list: D015415:Biomarkers
keywords: Autoimmune diseases; Compact bone; Hyposalivation; MSCs; Mesenchymal stromal cells; Nonobese Diabetic (NOD) mouse; Saliva; Sjogren’s syndrome; Sjogren’s-like disease; Xerostomia