title: Regulation of the Balance Between Proliferation and Differentiation in Germ Line Stem Cells.
abstract: In many animals, reproductive fitness is dependent upon the production of large numbers of gametes over an extended period of time. This level of gamete production is possible due to the continued presence of germ line stem cells. These cells can produce two types of daughter cells, self-renewing daughter cells that will maintain the stem cell population and differentiating daughter cells that will become gametes. A balance must be maintained between the proliferating self-renewing cells and those that differentiate for long-term gamete production to be maintained. Too little proliferation can result in depletion of the stem cell population, while too little differentiation can lead to a lack of gamete formation and possible tumor formation. In this chapter, we discuss our current understanding of how the balance between proliferation and differentiation is achieved in three well-studied germ line model systems: the Drosophila female, the mouse male, and the C. elegans hermaphrodite. While these three systems have significant differences in how this balance is regulated, including differences in stem cell population size, signaling pathways utilized, and the use of symmetric and/or asymmetric cell divisions, there are also similarities found between them. These similarities include the reliance on a predominant signaling pathway to promote proliferation, negative feedback loops to rapidly shutoff proliferation-promoting cues, close association of the germ line stem cells with a somatic niche, cytoplasmic connections between cells, projections emanating from the niche cell, and multiple mechanisms to limit the spatial influence of the niche. A comparison between different systems may help to identify elements that are essential for a proper balance between proliferation and differentiation to be achieved and elements that may be achieved through various mechanisms.
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, AB, Canada, T2N 1N4.
authors.forename: Ramya
authors.initials: R
authors.lastname: Singh
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, AB, Canada, T2N 1N4. dhansen@ucalgary.ca.
authors.forename: Dave
authors.initials: D
authors.lastname: Hansen
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D004330:Drosophila; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D008297:Male; D051379:Mice