title: Synthesis, Characterization and DNA-Binding Studies of Hydroxyl Functionalized Platinum(II) Salphen Complexes.
abstract: The platinum(II) salphen complex N,N'-Bis-4-(hydroxysalicylidene)-phenylenediamine-platinum(II); (1) and its two derivatives containing hydroxyl functionalized side chains N,N'-bis-[4-[[1-(2-hydroxyethoxy)] salicylidene] phenylenediamine-platinum(II); (2) and N,N'-bis-[4-[[1-(3-hydroxypropoxy)] salicylidene] phenylenediamine-platinum(II); (3) were synthesized and characterized. The structures of the complexes were confirmed by1H and13C NMR spectroscopy, FTIR, ESI-MS and CHN elemental analyses. The effects of the hydroxyl substituent on the spectral properties and the DNA binding behaviors of the Pt(II) complexes were explored. The binding mode and interactions of these complexes with duplex DNA (calf thymus DNA and porcine DNA) and also single-stranded DNA were studied by UV-Vis and emission DNA titration. The complexes interact with DNA by intercalation binding mode with the binding constants in the order of magnitude (Kb= 104M-1, CT-DNA) and (Kb= 105M-1, porcine DNA). The intercalation of the complex in the DNA structure was proposed to happen by π-π stacking due to its square-planar geometry and aromatic rings structure. The phosphorescence emission spectral characteristics of Pt(II) complexes when interacted with DNA have been studied. Also, the application of the chosen hydroxypropoxy side chains complex (3) as an optical DNA biosensor, specifically for porcine DNA was investigated. These findings will be valuable for the potential use of the platinum(II) salphen complex as an optical DNA biosensor for the detection of porcine DNA in food products.
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
authors.forename: Shahratul Ain
authors.initials: SA
authors.lastname: Mohd Sukri
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
authors.forename: Lee Yook
authors.initials: LY
authors.lastname: Heng
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia. nurulhuda@ukm.edu.my.
authors.forename: Nurul Huda
authors.initials: NH
authors.lastname: Abd Karim
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D056831:Coordination Complexes / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000378:metabolism*; D010655:Phenylenediamines / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D013552:Swine
chemical_list: D056831:Coordination Complexes; D010655:Phenylenediamines; C545695:salphen; D010984:Platinum; D004247:DNA
keywords: Calf thymus DNA; DNA binding; Optical DNA biosensor; Phosphorescence complex; Platinum(II) salphen; Porcine DNA