title: Nanoparticle-Based Discrimination of Single-Nucleotide Polymorphism in Long DNA Sequences.
abstract: Circulating DNA (ctDNA) and specifically the detection cancer-associated mutations in liquid biopsies promises to revolutionize cancer detection. The main difficulty however is that the length of typical ctDNA fragments (∼150 bases) can form secondary structures potentially obscuring the mutated fragment from detection. We show that an assay based on gold nanoparticles (65 nm) stabilized with DNA (Au@DNA) can discriminate single nucleotide polymorphism in clinically relevant ssDNA sequences (70-140 bases). The preincubation step was crucial to this process, allowing sequential bridging of Au@DNA, so that single base mutation can be discriminated, down to 100 pM concentration.
authors.affiliation: CIC biomaGUNE , Paseo de Miramón 182, 20014 Donostia-San Sebastián, Spain.|Oncology Area, Biodonostia Research Institute , 20014 Donostia-San Sebastián, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Sanromán-Iglesias
authors.affiliation: Oncology Area, Biodonostia Research Institute , 20014 Donostia-San Sebastián, Spain.|Ikerbasque, Basque Foundation for Science , 48013 Bilbao, Spain.
authors.forename: Charles H
authors.initials: CH
authors.lastname: Lawrie
authors.affiliation: CIC biomaGUNE , Paseo de Miramón 182, 20014 Donostia-San Sebastián, Spain.|Ikerbasque, Basque Foundation for Science , 48013 Bilbao, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN , 20014 Donostia-San Sebastián, Spain.
authors.forename: Luis M
authors.identifier: 0000-0002-6647-1353
authors.initials: LM
authors.lastname: Liz-Marzán
authors.affiliation: CIC biomaGUNE , Paseo de Miramón 182, 20014 Donostia-San Sebastián, Spain.|Ikerbasque, Basque Foundation for Science , 48013 Bilbao, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN , 20014 Donostia-San Sebastián, Spain.
authors.forename: Marek
authors.identifier: 0000-0002-3458-8450
authors.initials: M
authors.lastname: Grzelczak
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D006046:Gold / Q000737:chemistry*; D061925:Immobilized Nucleic Acids / Q000737:chemistry / Q000235:genetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide
chemical_list: D061925:Immobilized Nucleic Acids; D006046:Gold; D004247:DNA