title: In vitro-production of embryos using immature oocytes collected transvaginally from superstimulated wood bison (Bison bison athabascae).
abstract: Two experiments were done to test the hypothesis that morphologic characteristics of wood bison cumulus-oocyte complexes (COC) are reflective of the ability of the oocyte to develop to an advanced embryonic stage after in vitro maturation, fertilization and culture, and to determine the effect of prolonging the interval from the end of superstimulation treatment to oocyte collection (FSH starvation period). Experiments were done during the anovulatory season. In Experiment 1, ovarian superstimulation was induced in 10 bison with two doses of FSH given at 48 h intervals beginning at the time of follicular wave emergence. COC were collected 3 days (72 h) after the last dose of FSH by follicular aspiration and classified as compact, expanded or denuded. The COC were matured in vitro for 24 h before fertilization in vitro (Day 0). Embryo development was assessed on Days 3, 7 and 8. The blastocyst rate was 7/34, 2/10 and 0/3 in COC classified as compact, expanded and denuded, respectively; however, only compact COC resulted in embryos that reached the expanded blastocyst stage. In Experiment 2, COC were collected at either 3 or 4 days (72 or 96 h) after the last dose of FSH (n = 16 bison/group) to determine the effect of the duration of FSH starvation on oocyte competence. The COC were classified as compact good (>3 layers of cumulus cells), compact regular (1-3 layers of cumulus cells), expanded or denuded, and then matured, fertilized and cultured in vitro. Although follicles were larger (P < 0.05) in the 4-day FSH starvation group, there was no effect of starvation period on the distribution of COC morphology; overall, 112/194 (57.7%) were compact, 29/194 (26.3%) were expanded, 39/194 (20.1%) were denuded, and 14/194 (7.2%) were degenerated (P < 0.05). Similarly, there was no effect of starvation period on embryo development. Compact good COC had the highest cleavage (88%) and blastocyst rates (54%; P < 0.05), followed by compact regular COC at 73% and 25%, respectively. Expanded and denuded COC had low cleavage (40% vs. 59%, respectively) and blastocyst rates (5% vs. 8%, respectively). We conclude that morphologic characteristics of wood bison COC are reflective of the ability of the oocyte to develop into an embryo in vitro. Importantly, oocytes collected from superstimulated bison during the anovulatory season were competent to develop to the blastocyst stage following in vitro maturation, fertilization and culture.
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Miriam P
authors.initials: MP
authors.lastname: Cervantes
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.
authors.forename: J Manuel
authors.initials: JM
authors.lastname: Palomino
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research and Development Center, Saskatoon, Canada.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Anzar
authors.affiliation: Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Reuben J
authors.initials: RJ
authors.lastname: Mapletoft
authors.affiliation: Reproductive Physiology, Toronto Zoo, Ontario, Canada.
authors.forename: Gabriela F
authors.initials: GF
authors.lastname: Mastromonaco
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada. Electronic address: gregg.adams@usask.ca.
authors.forename: Gregg P
authors.initials: GP
authors.lastname: Adams
mesh_terms: D000818:Animals; D016164:Bison / Q000502:physiology*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005640:Follicle Stimulating Hormone; D054315:Oocyte Retrieval / Q000662:veterinary*; D009865:Oocytes / Q000502:physiology*; D010865:Pilot Projects; D013480:Superovulation
chemical_list: D005640:Follicle Stimulating Hormone
keywords: Blastocyst; COC morphology; FSH starvation; In vitro fertilization; In vitro maturation