title: Column switching UHPLC-MS/MS with restricted access material for the determination of CNS drugs in plasma samples.
abstract: BACKGROUND
  Polypharmacy is a common practice in schizophrenia. Consequently, therapeutic drug monitoring is usually adopted to maintain the concentrations of the drugs in the plasma within a targeted therapeutic range, to maximize therapeutic efficiency and to diminish adverse side effects.
  
  
  METHODS
  This study reports on a column switching UHPLC-MS/MS method to determine psychotropic drugs in plasma samples simultaneously.
  
  
  RESULTS
  The method was linear from 0.025 to 1.25 ng ml-1with R2above 0.9950 and the lack of fit test (p > 0.05). The precision values presented coefficients of variation lower than 12%, and the relative standard error of the accuracy were lower than 14%.
  
  
  CONCLUSIONS
  The column switching UHPLC-MS/MS method developed herein successfully determined drugs in schizophrenic patients' plasma samples for therapeutic drug monitoring.
authors.affiliation: Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Vinicius Ricardo
authors.initials: VR
authors.lastname: Acquaro
authors.affiliation: Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Diego Soares
authors.initials: DS
authors.lastname: Domingues
authors.affiliation: Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Maria Eugênia
authors.initials: ME
authors.lastname: Costa Queiroz
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D016903:Drug Monitoring; D064368:Healthy Volunteers; D006801:Humans; D057230:Limit of Detection; D011619:Psychotropic Drugs / Q000097:blood*; D015203:Reproducibility of Results; D012559:Schizophrenia / Q000097:blood / Q000188:drug therapy; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D011619:Psychotropic Drugs
keywords: C8-ADS column; column switching UHPLC–MS/MS; therapeutic drug monitoring