title: Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem Cell Injury by Reducing Hydroxyl Radical.
abstract: We examined whether consumption of hydrogen-rich water (HW) could ameliorate hematopoietic stem cell (HSC) injury in mice with total body irradiation (TBI). The results indicated that HW alleviated TBI-induced HSC injury with respect to cell number alteration and to the self-renewal and differentiation of HSCs. HW specifically decreased hydroxyl radical (∙OH) levels in the c-kit+cells of 4 Gy irradiated mice. Proliferative bone marrow cells (BMCs) increased and apoptotic c-kit+cells decreased in irradiated mice uptaken with HW. In addition, the mean fluorescence intensity (MFI) ofγ-H2AX and percentage of 8-oxoguanine positive cells significantly decreased in HW-treated c-kit+cells, indicating that HW can alleviate TBI-induced DNA damage and oxidative DNA damage in c-kit+cells. Finally, the cell cycle (P21), cell apoptosis (BCL-XL and BAK), and oxidative stress (NRF2, HO-1, NQO1, SOD, and GPX1) proteins were significantly altered by HW in irradiated mouse c-kit+cells. Collectively, the present results suggest that HW protects against TBI-induced HSC injury.
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Junling
authors.identifier: 0000-0003-4321-3156
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Xue
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Han
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Deguan
authors.initials: D
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Saijun
authors.identifier: 0000-0003-0691-6864
authors.initials: S
authors.lastname: Fan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D006859:Hydrogen / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D020011:Protective Agents / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control; D014867:Water / Q000737:chemistry*; D014916:Whole-Body Irradiation
chemical_list: D020011:Protective Agents; D014867:Water; D017665:Hydroxyl Radical; D006859:Hydrogen