title: Overexpression of MusaMYB31, a R2R3 type MYB transcription factor gene indicate its role as a negative regulator of lignin biosynthesis in banana.
abstract: Lignin and polyphenols are important cellular components biosynthesized through phenylpropanoid pathway. Phenylpropanoid pathway in plants is regulated by some important transcription factors including R2R3 MYB transcription factors. In this study, we report the cloning and functional characterization of a banana R2R3-MYB transcription factor (MusaMYB31) by overexpression in transgenic banana plants and evaluated its potential role in regulating biosynthesis of lignin and polyphenols. Sequence analysis of MusaMYB31 indicated its clustering with members of subgroup 4 (Sg4) of R2R3MYB family which are well known for their role as repressors of lignin biosynthesis. Expression analysis indicated higher expression of MusaMYB31 in corm and root tissue, known for presence of highly lignified tissue than other organs of banana. Overexpression of MusaMYB31 in banana cultivar Rasthali was carried out and four transgenic lines were confirmed by GUS histochemical staining, PCR analysis and Southern blot. Histological and biochemical analysis suggested reduction of cell wall lignin in vascular elements of banana. Transgenic lines showed alteration in transcript levels of general phenylpropanoid pathway genes including lignin biosynthesis pathway genes. Reduction of total polyphenols content in transgenic lines was in line with the observation related to repression of general phenylpropanoid pathway genes. This study suggested the potential role of MusaMYB31 as repressor of lignin and polyphenols biosynthesis in banana.
authors.affiliation: Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.|Homi Bhabha National Institute, Anushakti Nagar, Mumbai, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Tak
authors.affiliation: Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.|Homi Bhabha National Institute, Anushakti Nagar, Mumbai, India.
authors.forename: Sanjana
authors.initials: S
authors.lastname: Negi
authors.affiliation: Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
authors.forename: T R
authors.initials: TR
authors.lastname: Ganapathi
mesh_terms: D000595:Amino Acid Sequence; D002473:Cell Wall / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D020570:Genes, myb; D008031:Lignin / Q000096:biosynthesis; D028521:Musa / Q000235:genetics* / Q000378:metabolism; D010666:Phenylpropionates / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D059808:Polyphenols / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010666:Phenylpropionates; D010940:Plant Proteins; D059808:Polyphenols; D012333:RNA, Messenger; D014157:Transcription Factors; D008031:Lignin