title: Lamin A overexpression promotes osteoblast differentiation and calcification in the MC3T3-E1 preosteoblastic cell line.
abstract: Lamin A/C is a component of the nuclear lamina, which is involved in cellular proliferation and differentiation. However, the mechanism by which lamin A regulates osteoblast differentiation is not well understood. In this study, we investigated lamin A/C expression during osteoblast differentiation in a preosteoblastic cell line, MC3T3-E1. Real-time PCR analysis showed that lamin A/C mRNA expression was upregulated during BMP-2 induced osteoblast differentiation. Treatment with the estrogen receptor antagonist, fulvestrant, inhibited osteoblast differentiation and the upregulation of lamin A/C mRNA and protein expressions in the presence of BMP-2. These results clearly demonstrated that lamin A/C expression correlates with osteoblast differentiation. To determine the roles of lamin A expression in osteoblast differentiation, MC3T3-E1 cells were transfected with a vector overexpressing lamin A. Results showed that lamin A overexpression promoted osteoblast differentiation and calcification by inducing the expression of alkaline phosphatase, type 1 collagen, BSP, osteocalcin, and DMP-1 in the presence of BMP-2. Furthermore, lamin A overexpression partially restored osteoblastic capacity in the presence of fulvestrant by increasing the expression of BSP, osteocalcin, and DMP-1. These results suggest that lamin A plays important roles in maintaining the osteoblast differentiation and function.
authors.affiliation: Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Tsukune
authors.affiliation: Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan. Electronic address: naitou.masako@nihon-u.ac.jp.
authors.forename: Masako
authors.initials: M
authors.lastname: Naito
authors.affiliation: Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kubota
authors.affiliation: Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Ozawa
authors.affiliation: Division of Applied Oral Science, Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Nagao
authors.affiliation: Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Ohashi
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan; Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sato
authors.affiliation: Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Tomihisa
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002478:Cells, Cultured; D034904:Lamin Type A / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*
chemical_list: D034904:Lamin Type A; C440308:lamin C
keywords: BMP-2; Fulvestrant; Lamin A/C; Nuclear lamina; Osteoblast differentiation