title: Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach.
abstract: Brachypodiumdistachyonis a suitable plant model for studying temperate cereal crops, such as wheat, barley or rice, and helpful in the study of the grain cell wall. Indeed, the most abundant hemicelluloses that are in theB. distachyoncell wall of grain are (1-3)(1-4)-β-glucans and arabinoxylans, in a ratio similar to those of cereals such as barley or oat. Conversely, these cell walls contain few pectins and xyloglucans. Cell walls play an important role in grain physiology. The modifications of cell wall polysaccharides that occur during grain development and filling are key in the determination of the size and weight of the cereal grains. The mechanisms required for cell wall assembly and remodelling are poorly understood, especially in cereals. To provide a better understanding of these processes, we purified the cell wall at three developmental stages of theB. distachyongrain. The proteins were then extracted, and a quantitative and comparative LC-MS/MS analysis was performed to investigate the protein profile changes during grain development. Over 466 cell wall proteins (CWPs) were identified and classified according to their predicted functions. This work highlights the different proteome profiles that we could relate to the main phases of grain development and to the reorganization of cell wall polysaccharides that occurs during these different developmental stages. These results provide a good springboard to pursue functional validation to better understand the role of CWPs in the assembly and remodelling of the grain cell wall of cereals.
authors.affiliation: UR1268 BIA (Biopolymères Interactions Assemblages), INRA, Nantes 44300, France. mfrancin@nantes.inra.fr.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Francin-Allami
authors.affiliation: UR1268 BIA (Biopolymères Interactions Assemblages), INRA, Nantes 44300, France. virginie.lollier@nantes.inra.fr.
authors.forename: Virginie
authors.initials: V
authors.lastname: Lollier
authors.affiliation: UR1268 BIA (Biopolymères Interactions Assemblages), INRA, Nantes 44300, France. marapavlovic86@gmail.com.
authors.forename: Marija
authors.initials: M
authors.lastname: Pavlovic
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 Chemin de Borderouge-Auzeville, BP42617, Castanet-Tolosan 31326, France. sancle@lrsv.ups-tlse.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: San Clemente
authors.affiliation: UR1268 BIA (Biopolymères Interactions Assemblages), INRA, Nantes 44300, France. helene.rogniaux@nantes.inra.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Rogniaux
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 Chemin de Borderouge-Auzeville, BP42617, Castanet-Tolosan 31326, France. jamet@lrsv.ups-tlse.fr.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Jamet
authors.affiliation: UR1268 BIA (Biopolymères Interactions Assemblages), INRA, Nantes 44300, France. Fabienne.Guillon@nantes.inra.fr.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Guillon
authors.affiliation: UR1268 BIA (Biopolymères Interactions Assemblages), INRA, Nantes 44300, France. colette.larre@nantes.inra.fr.
authors.forename: Colette
authors.initials: C
authors.lastname: Larré
keywords: Brachypodium distachyon; Pooideae; cell wall; cell wall proteins; grain development; grass; polysaccharide remodelling; proteomics; quantitative mass spectrometry