title: Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers ofPueraria tuberosa.
abstract: Inflammation is the major causative factor of different diseases such as cardiovascular disease, diabetes, obesity, osteoporosis, rheumatoid arthritis, inflammatory bowel disease, and cancer. Anti-inflammatory drugs are often the first step of treatment in many of these diseases. The present study is aimed at evaluating the anti-inflammatory properties of isoorientin, a selective cyclooxygenase-2 (COX-2) inhibitor isolated from the tubers ofPueraria tuberosa, in vitro on mouse macrophage cell line (RAW 264.7) and in vivo on mouse paw edema and air pouch models of inflammation. Isoorientin reduced inflammation in RAW 264.7 cell line in vitro and carrageenan induced inflammatory animal model systems in vivo. Cellular infiltration into pouch tissue was reduced in isoorientin treated mice compared to carrageenan treated mice. Isoorientin treated RAW 264.7 cells and animals showed reduced expression of inflammatory proteins like COX-2, tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), 5-lipoxygenase (5-LOX), and interleukin 1-β(IL-1-β) both in vitro and in vivo. The antioxidant enzyme levels of catalase and GST were markedly increased in isoorientin treated mice compared to carrageenan treated mice. These results suggest that isoorientin, a selective inhibitor of COX-2, not only exerts anti-inflammatory effects in LPS induced RAW cells and carrageenan induced inflammatory model systems but also exhibits potent antioxidant properties.
authors.affiliation: School of Life Sciences, University of Hyderabad, Hyderabad 500046, India; National Institute of Animal Biotechnology, Hyderabad 500049, India.
authors.forename: Kotha
authors.initials: K
authors.lastname: Anilkumar
authors.affiliation: School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: Gorla V
authors.initials: GV
authors.lastname: Reddy
authors.affiliation: School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: Rajaram
authors.initials: R
authors.lastname: Azad
authors.affiliation: Department of Biochemistry and Bioinformatics, School of Life Sciences, Institute of Science, GITAM University, Visakhapatnam, Andhra Pradesh 530 045, India.
authors.forename: Nagendra Sastry
authors.initials: NS
authors.lastname: Yarla
authors.affiliation: School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: Gangappa
authors.initials: G
authors.lastname: Dharmapuri
authors.affiliation: National Institute of Animal Biotechnology, Hyderabad 500049, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia; Enzymoics, 7 Peterlee Place, Hebersham, NSW 2770, Australia; Novel Global Community Educational Foundation, Hebersham, NSW, Australia.
authors.forename: Mohammad A
authors.identifier: 0000-0003-0088-0565
authors.initials: MA
authors.lastname: Kamal
authors.affiliation: School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: Reddanna
authors.identifier: 0000-0001-6343-6758
authors.initials: R
authors.lastname: Pallu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002351:Carrageenan / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology*; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D047311:Luteolin / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D035281:Plant Tubers / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D002351:Carrageenan; C057912:homoorientin; D047311:Luteolin