title: Uranium XAFS analysis of kidney from rats exposed to uranium.
abstract: The kidney is the critical target of uranium exposure because uranium accumulates in the proximal tubules and causes tubular damage, but the chemical nature of uranium in kidney, such as its chemical status in the toxic target site, is poorly understood. Micro-X-ray absorption fine-structure (µXAFS) analysis was used to examine renal thin sections of rats exposed to uranyl acetate. The U LIII-edge X-ray absorption near-edge structure spectra of bulk renal specimens obtained at various toxicological phases were similar to that of uranyl acetate: their edge position did not shift compared with that of uranyl acetate (17.175 keV) although the peak widths for some kidney specimens were slightly narrowed. µXAFS measurements of spots of concentrated uranium in the micro-regions of the proximal tubules showed that the edge jump slightly shifted to lower energy. The results suggest that most uranium accumulated in kidney was uranium (VI) but a portion might have been biotransformed in rats exposed to uranyl acetate.
authors.affiliation: Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kitahara
authors.affiliation: Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
authors.forename: Chiya
authors.initials: C
authors.lastname: Numako
authors.affiliation: Japan Synchrotron Radiation Research Institute, Mikazuki, Hyogo 679-5198, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Terada
authors.affiliation: Japan Synchrotron Radiation Research Institute, Mikazuki, Hyogo 679-5198, Japan.
authors.forename: Kiyohumi
authors.initials: K
authors.lastname: Nitta
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Shimada
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Shino
authors.initials: S
authors.lastname: Homma-Takeda
mesh_terms: D000818:Animals; D007668:Kidney / Q000187:drug effects; D007687:Kidney Tubules, Proximal; D051381:Rats; D014501:Uranium / Q000633:toxicity
chemical_list: D014501:Uranium
keywords: elemental mapping; kidney; uranium; µXAFS