title: Synthesis of hydroxyethyl-methacrylate-(L)-histidine methyl ester cryogels. Application on the separation of bovine immunoglobulin G.
abstract: In this study cryogels based 2-hydroxyethyl methacrylate (HEMA) functionalized with N-methacryloyl-L-histidine methyl ester (MAH) were synthesized and used for the adsorption and separation of bovine IgG. Two series of cryogels functionalized with 5 and 10 mg of MAH as pseudobioaffinity ligand were prepared and characterized by swelling test, FTIR and SEM analysis. The adsorption efficiency of the bovine immunoglobulin into cryogels is discussed with respect to the following chromatographic parameters: pH, flow rate, initial IgG concentration, adsorption time and ionic strength. Our results show good adsorption of bovine immunoglobulin under mild separation conditions at pH 7.4. The maximum binding capacity was determined (32.4 mg/g of cryogel) and demonstrates the efficiency of the used cryogels. This efficacy is clearly seen upon increasing the maximum binding capacity from 23.2 mg (obtained with cryogels with 5 mg MAH) to 32.4 mg/g (for cryogel with 10 mg MAH ligand concentration). The purity of separated fractions was evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Together our observations highlights poly (HEMA-MAH) as an efficient adsorbent for bovine immunoglobulins G separation.
authors.affiliation: Laboratorire de Valorisation des Ressources Naturelles et Produits de Santé (LVRNPS), Doctoral School of Sciences and Technology, Lebanese University, Rafic Hariri University Campus, P.O. BOX 14/6573, Hadath, Lebanon. Electronic address: aelkak@ul.edu.lb.
authors.forename: Assem
authors.initials: A
authors.lastname: Elkak
authors.affiliation: Laboratorire de Valorisation des Ressources Naturelles et Produits de Santé (LVRNPS), Doctoral School of Sciences and Technology, Lebanese University, Rafic Hariri University Campus, P.O. BOX 14/6573, Hadath, Lebanon.
authors.forename: Amar
authors.initials: A
authors.lastname: Hamade
authors.affiliation: Faculty of Science, Department of Chemistry, Biochemistry Division, Hacettepe University, 06532 Beytepe-Ankara, Turkey.
authors.forename: Nilay
authors.initials: N
authors.lastname: Bereli
authors.affiliation: Faculty of Science, Department of Chemistry, Biochemistry Division, Hacettepe University, 06532 Beytepe-Ankara, Turkey.
authors.forename: Canan
authors.initials: C
authors.lastname: Armutcu
authors.affiliation: Faculty of Science, Department of Chemistry, Biochemistry Division, Hacettepe University, 06532 Beytepe-Ankara, Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D059985:Cryogels / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification*; D009994:Osmolar Concentration; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*
chemical_list: D059985:Cryogels; D007074:Immunoglobulin G; D011102:Polyhydroxyethyl Methacrylate; D006639:Histidine; C053861:histidine methyl ester
keywords: Bovine; Cryogels; IgG; MAH ligand; Pseudobioaffinity; Separation