title: Miniature Dielectric Barrier Discharge Nonthermal Plasma Induces Apoptosis in Lung Cancer Cells and Inhibits Cell Migration.
abstract: Traditional cancer treatments like radiotherapy and chemotherapy have drawbacks and are not selective for killing only cancer cells. Nonthermal atmospheric pressure plasmas with dielectric barrier discharge (DBD) can be applied to living cells and tissues and have emerged as novel tools for localized cancer therapy. The purpose of this study was to investigate the different effects caused by miniature DBD (mDBD) plasma to A549 lung cancer cells. In this study, A549 lung cancer cells cultured in 12 well plates were treated with mDBD plasma for specified treatment times to assess the changes in the size of the area of cell detachment, the viability of attached or detached cells, and cell migration. Furthermore, we investigated an innovative mDBD plasma-based therapy for localized treatment of lung cancer cells through apoptotic induction. Our results indicate that plasma treatment for 120 sec causes apoptotic cell death in 35.8% of cells, while mDBD plasma treatment for 60 sec, 30 sec, or 15 sec causes apoptotic cell death in 20.5%, 14.1%, and 6.3% of the cell population, respectively. Additionally, we observed reduced A549 cell migration in response to mDBD plasma treatment. Thus, mDBD plasma system can be a viable platform for localized lung cancer therapy.
authors.affiliation: Department of Bioengineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA.
authors.forename: Surya B
authors.identifier: 0000-0002-8595-3219
authors.initials: SB
authors.lastname: Karki
authors.affiliation: Department of Bioengineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA; Department of Orthopaedic Surgery, University of Toledo Medical Center, Toledo, OH 43614, USA.
authors.forename: Eda
authors.identifier: 0000-0003-3275-167X
authors.initials: E
authors.lastname: Yildirim-Ayan
authors.affiliation: Department of Biochemistry and Cancer Biology, University of Toledo Health Science Campus, Toledo, OH 43614, USA.
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Eisenmann
authors.affiliation: Department of Bioengineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA; Department of Mechanical, Industrial, and Manufacturing Engineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA.
authors.forename: Halim
authors.identifier: 0000-0003-4103-9772
authors.initials: H
authors.lastname: Ayan
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis / Q000187:drug effects*; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004560:Electricity; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008856:Microscopy, Fluorescence; D058626:Plasma Gases / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D058626:Plasma Gases