title: Generation of LIF-independent induced pluripotent stem cells from canine fetal fibroblasts.
abstract: Takahashi and Yamanaka established the first technique in which transcription factors related to pluripotency are incorporated into the genome of somatic cells to enable reprogramming of these cells. The expression of these transcription factors enables a differentiated somatic cell to reverse its phenotype to an embryonic state, generating induced pluripotent stem cells (iPSCs). iPSCs from canine fetal fibroblasts were produced through lentiviral polycistronic human and mouse vectors (hOSKM/mOSKM), aiming to obtain pluripotent stem cells with similar features to embryonic stem cells (ESC) in this animal model. The cell lines obtained in this study were independent of LIF or any other supplemental inhibitors, resistant to enzymatic procedure (TrypLE Express Enzyme), and dependent on bFGF. Clonal lines were obtained from slightly different protocols with maximum reprogramming efficiency of 0.001%. All colonies were positive for alkaline phosphatase, embryoid body formation, and spontaneous differentiation and expressed high levels of endogenous OCT4 and SOX2. Canine iPSCs developed tumors at 120 days post-injection in vivo. Preliminary chromosomal evaluations were performed by FISH hybridization, revealing no chromosomal abnormality. To the best of our knowledge, this report is the first to describe the ability to reprogram canine somatic cells via lentiviral vectors without supplementation and with resistance to enzymatic action, thereby demonstrating the pluripotency of these cell lines.
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, FZEA/USP, São Paulo, Brazil.
authors.forename: N J N
authors.initials: NJN
authors.lastname: Gonçalves
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, FZEA/USP, São Paulo, Brazil; Department of Veterinary Surgery, Sector Anatomy, Faculty of Veterinary Medicine and Animal Science, FMVZ/USP, São Paulo, Brazil.
authors.forename: F F
authors.initials: FF
authors.lastname: Bressan
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, FZEA/USP, São Paulo, Brazil.
authors.forename: K C S
authors.initials: KCS
authors.lastname: Roballo
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, FZEA/USP, São Paulo, Brazil; Department of Veterinary Surgery, Sector Anatomy, Faculty of Veterinary Medicine and Animal Science, FMVZ/USP, São Paulo, Brazil.
authors.forename: F V
authors.initials: FV
authors.lastname: Meirelles
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, FZEA/USP, São Paulo, Brazil.
authors.forename: P L P
authors.initials: PLP
authors.lastname: Xavier
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, FZEA/USP, São Paulo, Brazil.
authors.forename: H
authors.initials: H
authors.lastname: Fukumasu
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
authors.forename: C
authors.initials: C
authors.lastname: Williams
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
authors.forename: M
authors.initials: M
authors.lastname: Breen
authors.affiliation: Department of Cell Biology, Duke University, Durham, NC, USA.
authors.forename: S
authors.initials: S
authors.lastname: Koh
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sper
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA; Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA.
authors.forename: J
authors.initials: J
authors.lastname: Piedrahita
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, FZEA/USP, São Paulo, Brazil; Department of Veterinary Surgery, Sector Anatomy, Faculty of Veterinary Medicine and Animal Science, FMVZ/USP, São Paulo, Brazil. Electronic address: ceambrosio@usp.br.
authors.forename: C E
authors.initials: CE
authors.lastname: Ambrósio
mesh_terms: D000818:Animals; D004285:Dogs; D005333:Fetus / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D039904:Pluripotent Stem Cells / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D053605:Leukemia Inhibitory Factor
keywords: Canine; Cellular reprogramming; Pluripotency; Stem cells; iPSC