title: Influence of 3 different forms of a commercially available denture adhesive material on the growth of Candida species: An in vitro study.
abstract: BACKGROUND
  Although different forms of denture adhesives are widely used by the elderly, insufficient information is available for the effect of adhesives on the growth of Candida species.
  
  
  OBJECTIVE
  The purpose of this in vitro study was to compare the influence of 3 different forms of a commercially available denture adhesive material on the growth of Candida species by monitoring the pH and number of colonies in the growth medium at different incubation periods.
  
  
  METHODS
  A total of 160 specimens of acrylic resin sheets of standard dimensions were fabricated and divided into 4 groups of 40 specimens each. The first group of 40 specimens was used as a control to which no adhesives were added. To the remaining groups, powder, cream, and strip forms of secure denture adhesives were added. After the addition of standard strains of Candida, 10 specimens from each group were then incubated for a specific period of 6 hours, 24 hours, 48 hours, and 120 hours. The pH and the number of colonies in the medium were recorded for each specimen at its specific incubation period. The data obtained were statistically analyzed using ANOVA and the Tukey honest significant differences post hoc test (α=.05).
  
  
  RESULTS
  The specimens with different forms of adhesive showed a significant (P<.001) increase in the pH of the medium (pH 4.70 to 5.37) compared with the specimens without adhesives (pH 3.83 to 4.65). The specimens with adhesives also showed a reduction in the number of Candida colonies (216.50 to 388.10×103cells/μL) compared with the specimens without adhesives (325.90 to 400.50×103cells/μL).
  
  
  CONCLUSIONS
  All forms of denture adhesives tested exhibited an antifungal effect. Prolonged antifungal effect was shown by the strip form of adhesives.
authors.affiliation: Senior lecturer, Department of Prosthodontics, Azeezia College of Dental Sciences and Research, Kerala, India.
authors.forename: Anuram
authors.initials: A
authors.lastname: Rajaram
authors.affiliation: Professor, Department of Prosthodontics, Azeezia College of Dental Sciences and Research, Kerala, India. Electronic address: drsmitamanoj@yahoo.co.in.
authors.forename: Smita Sara
authors.initials: SS
authors.lastname: Manoj
mesh_terms: D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D003738:Dental Cements / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques
chemical_list: D003738:Dental Cements