title: Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip.
abstract: This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS). The nanoparticles were fabricated as beta-cyclodextrin-coated silver nanoparticles (Ag@CD NPs) modified with para-aminothiophenol (p-ATP) and folic acid (FA) on the surface. The p-ATP molecules act as the Raman reporter while the FA tags have high affinity for folate receptors (FR) that are over-expressed on the surface cancerous cells, so that the nanoparticles can enter the cells and be monitored by the Raman reporter. Therefore, the nanoparticles could be utilized not only as cell invaders due to endocytosis but also as a SERS sensitive probe to monitor the effect of FR-targeted drugs such as dihydroartemisinin (DHA) that induce the population change of FR on the membrane of living cells. As a result, we have successfully demonstrated that we are able to employ the Ag@CD@p-ATP@FA NPs to evaluate the number of NPs entering living cells quantitatively and correspondingly the drug effect on cancer cells in a well-controlled way.
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China. huangq@ipp.ac.cn and University of Science and Technology of China, Hefei 230026, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Zhai
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China. huangq@ipp.ac.cn and University of Science and Technology of China, Hefei 230026, China and School of Physical Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Fengqiu
authors.initials: F
authors.lastname: Zhang
authors.affiliation: University of Science and Technology of China, Hefei 230026, China.
authors.forename: Xiangyu
authors.identifier: 0000-0002-3618-5954
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China. huangq@ipp.ac.cn and University of Science and Technology of China, Hefei 230026, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhong
authors.affiliation: University of Science and Technology of China, Hefei 230026, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: University of Science and Technology of China, Hefei 230026, China.
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China. huangq@ipp.ac.cn and University of Science and Technology of China, Hefei 230026, China.
authors.forename: Qing
authors.identifier: 0000-0002-8884-2063
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D037621:Artemisinins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005492:Folic Acid / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D056656:Lab-On-A-Chip Devices; D053768:Metal Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012834:Silver / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D037621:Artemisinins; D012834:Silver; C039060:artenimol; D005492:Folic Acid