title: Isoelectric Point Separations of Peptides and Proteins.
abstract: The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies.
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA. mperga2@uic.edu.
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Pergande
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA. cologna@uic.edu.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Cologna
keywords: capillary isoelectric focusing; electrophoresis; isoelectric focusing; isoelectric trapping; proteomics; two-dimensional gel electrophoresis