title: Inhibition of microRNA-153 protects neurons against ischemia/reperfusion injury in an oxygen-glucose deprivation and reoxygenation cellular model by regulating Nrf2/HO-1 signaling.
abstract: MicroRNAs are emerging as critical regulators in cerebral ischemia/reperfusion injury; however, their exact roles remain poorly understood. miR-153 is reported to be a neuron-related miRNA involved in neuroprotection. In this study, we aimed to investigate the precise role of miR-153 in regulating neuron survival during cerebral ischemia/reperfusion injury using an oxygen-glucose deprivation and reoxygenation (OGD/R) cellular model. We found that miR-153 was significantly upregulated in neurons subjected to OGD/R treatment. Inhibition of miR-153 significantly attenuated OGD/R-induced injury and oxidative stress in neurons. Nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a target gene of miR-153. Inhibition of miR-153 significantly promoted the expression of Nrf2 and heme oxygenase-1 (HO-1). However, silencing of Nrf2 significantly blocked the protective effects of miR-153 inhibition. Our study indicates that the inhibition of miR-153 protects neurons against OGD/R-induced injury by regulating Nrf2/HO-1 signaling and suggests a potential therapeutic target for cerebral ischemia/reperfusion injury.
authors.affiliation: Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.|Department of Neonatology, Frist Hospital of Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Department of Pediatrics Neurology, Frist Hospital of Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Gao
authors.affiliation: Cadre's Ward of Frist Hospital of Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Canxia
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yao
authors.affiliation: Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
mesh_terms: D005947:Glucose / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008959:Models, Neurological; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000235:genetics; D010100:Oxygen / Q000378:metabolism; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D015398:Signal Transduction
chemical_list: C556500:MIRN153 microRNA, human; D035683:MicroRNAs; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D005947:Glucose; D010100:Oxygen
keywords: Cerebral ischemia/reperfusion injury; HO-1; Nrf2; miR-153