title: Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells.
abstract: Understanding muscle stem cell behaviors can potentially provide insights into how these cells act and respond during normal growth and diseased contexts. The zebrafish is an ideal model organism to examine these behaviors in vivo where it would normally be technically challenging in other mammalian models. This chapter will describe the procedures required to successfully conduct live imaging of zebrafish transgenics that has specifically been adapted for skeletal muscle.
authors.affiliation: Australian Regenerative Medicine Institute, Monash University, 15 Innovation Walk, Clayton, VIC, 3800, Australia.
authors.forename: Phong D
authors.initials: PD
authors.lastname: Nguyen
authors.affiliation: Australian Regenerative Medicine Institute, Monash University, 15 Innovation Walk, Clayton, VIC, 3800, Australia. peter.currie@monash.edu.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Currie
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods; D018482:Muscle, Skeletal / Q000166:cytology; D009132:Muscles / Q000166:cytology*; D010641:Phenotype; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D015415:Biomarkers
keywords: Live imaging; Muscle progenitor; Satellite cell; Skeletal muscle; Stem cell; Timelapse; Transgenics; Zebrafish