title: Isolation of Stromal Stem Cells from Adipose Tissue.
abstract: Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium.
authors.affiliation: Department of Health Sciences, Università del Piemonte Orientale "A.Avogadro", Via Solaroli, 17, 28100, Novara, Italy. maria.prat@med.uniupo.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Prat
authors.affiliation: Department of Health Sciences, Università del Piemonte Orientale "A.Avogadro", Via Solaroli, 17, 28100, Novara, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Oltolina
authors.affiliation: Department of Health Sciences, Università del Piemonte Orientale "A.Avogadro", Via Solaroli, 17, 28100, Novara, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Antonini
authors.affiliation: Department of Health Sciences, Università del Piemonte Orientale "A.Avogadro", Via Solaroli, 17, 28100, Novara, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Zamperone
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence
chemical_list: D015415:Biomarkers
keywords: Adipose tissue; Cell culturing; Cell differentiation; Cell isolation; Immunophenotyping; Mesenchymal stem cells; Stromal stem cells