title: Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection.
abstract: Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
authors.affiliation: Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Katie A
authors.initials: KA
authors.lastname: Mitzelfelt
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: mcdec@mail.med.upenn.edu.
authors.forename: Chris
authors.initials: C
authors.lastname: McDermott-Roe
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Michael N
authors.initials: MN
authors.lastname: Grzybowski
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Maribel
authors.initials: M
authors.lastname: Marquez
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Chieh-Ti
authors.initials: CT
authors.lastname: Kuo
authors.affiliation: PharmaCell, 6229 Maastricht, the Netherlands.
authors.forename: Michael
authors.initials: M
authors.lastname: Riedel
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Shuping
authors.initials: S
authors.lastname: Lai
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Melinda J
authors.initials: MJ
authors.lastname: Choi
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Kurt D
authors.initials: KD
authors.lastname: Kolander
authors.affiliation: Division of Genetics, Department of Pediatrics, Human Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Helbling
authors.affiliation: Division of Genetics, Department of Pediatrics, Human Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: Dimmock
authors.affiliation: Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Michele A
authors.initials: MA
authors.lastname: Battle
authors.affiliation: Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA.
authors.forename: Chuanchau J
authors.initials: CJ
authors.lastname: Jou
authors.affiliation: Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA.
authors.forename: Martin
authors.initials: M
authors.lastname: Tristani-Firouzi
authors.affiliation: Section of Quantitative Health Sciences, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: James W
authors.initials: JW
authors.lastname: Verbsky
authors.affiliation: Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Ivor J
authors.initials: IJ
authors.lastname: Benjamin
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address: ageurts@mcw.edu.
authors.forename: Aron M
authors.initials: AM
authors.lastname: Geurts
mesh_terms: D064113:CRISPR-Cas Systems; D002460:Cell Line; D059766:DNA End-Joining Repair; D000072669:Gene Editing; D055879:Gene Knock-In Techniques; D018390:Gene Targeting; D005822:Genetic Vectors; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D059767:Recombinational DNA Repair