title: Therapeutic potential of adipose-derived stem cells and macrophages for ischemic skeletal muscle repair.
abstract: Progressive ischemia due to peripheral artery disease causes muscle damage and reduced strength of the lower extremities. Autologous cell therapy is an attractive treatment to restore perfusion and improve muscle function. Adipose-derived stem cells (ASCs) have therapeutic potential in tissue repair, including polarizing effects on macrophages (MPs).
  
  
  
  Co-culture systems of ASCs and MPs were analyzed for gene and protein expression modifications in ASC-conditioned MPs. Co-transplantation of MPs/ASCs in vivo led to improved skeletal muscle regeneration in a mouse model of peripheral artery disease.
  
  
  
  ASCs/MPs therapy restored muscle function, increased perfusion and reduced inflammatory infiltrate.
  
  
  
  Combined MPs/ASCs cell therapy is a promising approach to restore muscle function and stimulate local angiogenesis in the ischemic limb.
authors.affiliation: Biomedical Engineering, Cockrell School of Engineering, The University of Texas at Austin, 107 W Dean Keeton, Austin, TX 78712, USA.
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Rybalko
authors.affiliation: Department of Kinesiology, The University of Texas at Austin, 1 University Station D3700, Austin, TX 78712, USA.
authors.forename: Pei-Ling
authors.initials: PL
authors.lastname: Hsieh
authors.affiliation: Biomedical Engineering, Cockrell School of Engineering, The University of Texas at Austin, 107 W Dean Keeton, Austin, TX 78712, USA.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Ricles
authors.affiliation: Biomedical Engineering, Cockrell School of Engineering, The University of Texas at Austin, 107 W Dean Keeton, Austin, TX 78712, USA.
authors.forename: Eunna
authors.initials: E
authors.lastname: Chung
authors.affiliation: Department of Kinesiology, The University of Texas at Austin, 1 University Station D3700, Austin, TX 78712, USA.
authors.forename: Roger P
authors.initials: RP
authors.lastname: Farrar
authors.affiliation: Biomedical Engineering, Cockrell School of Engineering, The University of Texas at Austin, 107 W Dean Keeton, Austin, TX 78712, USA.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Suggs
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D018920:Coculture Techniques; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007511:Ischemia / Q000628:therapy*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000166:cytology* / Q000293:injuries; D018919:Neovascularization, Physiologic; D058729:Peripheral Arterial Disease / Q000628:therapy; D012038:Regeneration / Q000502:physiology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
keywords: adipose-derived stem cells; cell-mediated therapy; ischemic injury; macrophages; peripheral artery disease; skeletal muscle regeneration