title: Association of A1538G and C2437T single nucleotide polymorphisms in heat shock protein-70 genes with diabetic nephropathy among South Indian population.
abstract: Diabetic Nephropathy (DN) is the leading cause of end-stage renal disease, characterized by progressive albuminuria and conferring additional risk of cardiovascular disease (CVD) and mortality. The crucial role of heat-shock proteins (HSPs) on renal function in patients with DN has been well documented. The present study was aimed to understand the association ofHSP-70gene variants on the susceptibility of Type 2 Diabetes Mellitus (T2DM) and DN. A total of 946 subjects (549 Males; 397 Females) were recruited and divided into four groups according to the levels of urinary albumin excretion (UAE): those with normoalbuminuria (UAE <30 mg/24 h;n=230), those with microalbuminuria (30≤ UAE ≤300 mg/24 h;n=230), and those with macroalbuminuria (UAE> 300 mg/24 h;n=230). The control group randomly enrolled a consecutive population of 256 healthy subjects who had a routine medical check-up in our hospital. Those subjects had no history or clinical symptoms of diabetes. Subjects were genotyped forHSP70-2(+1538 A/G; rs2763979) andHSP70-hom(+2437 C/T; rs2227956) by PCR-restriction fragment length polymorphism (RFLP). The 'G' allele ofHSP70-2(+1538 A/G) single nucleotide polymorphism (SNP) showed relative risk for normoalbuminuria, microalbuminuria and macroalbuminuria subjects whereas the 'T' allele ofHSP70-hom(+2437 C/T) SNP showed significant protection against macroalbuminuria subjects. In conclusion, our results indicate that theHSP70-2(+1538 A/G) andHSP70-hom(+2437 C/T) SNPs are highly associated with renal complications in T2DM among the South Indian population.
authors.affiliation: Life Science Division, SRM Research Institute, SRM University, Kattankulathur, Chennai-603203, India.
authors.forename: Umapathy
authors.initials: U
authors.lastname: Dhamodharan
authors.affiliation: Department of Biochemistry and Molecular Genetics, Prof. M. Viswanathan Diabetes Research Centre and M.V. Hospital for Diabetes (A WHO Collaborating Centre for Research, Education & Training in Diabetes), Royapuram, Chennai-600013, India.
authors.forename: Krishnamoorthy
authors.initials: K
authors.lastname: Ezhilarasi
authors.affiliation: Life Science Division, SRM Research Institute, SRM University, Kattankulathur, Chennai-603203, India.
authors.forename: Balashanmugam
authors.initials: B
authors.lastname: Ponjayanthi
authors.affiliation: Life Science Division, SRM Research Institute, SRM University, Kattankulathur, Chennai-603203, India.
authors.forename: Dornadula
authors.initials: D
authors.lastname: Sireesh
authors.affiliation: Life Science Division, SRM Research Institute, SRM University, Kattankulathur, Chennai-603203, India drvijay@mvdiabetes.com ramkumar.km@res.srmuniv.ac.in.
authors.forename: Kunka Mohanram
authors.initials: KM
authors.lastname: Ramkumar
authors.affiliation: Department of Biochemistry and Molecular Genetics, Prof. M. Viswanathan Diabetes Research Centre and M.V. Hospital for Diabetes (A WHO Collaborating Centre for Research, Education & Training in Diabetes), Royapuram, Chennai-600013, India drvijay@mvdiabetes.com ramkumar.km@res.srmuniv.ac.in.
authors.forename: Vijay
authors.initials: V
authors.lastname: Viswanathan
mesh_terms: D000328:Adult; D000368:Aged; D000419:Albuminuria / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000453:epidemiology; D003928:Diabetic Nephropathies / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000097:blood / Q000235:genetics*; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D024101:Mitochondrial Proteins
keywords: Diabetic Nephropathy; Heat-shock proteins; Macroalbuminuria; Microalbuminuria; Single Nucleotide Polymorphism; Type 2 Diabetes Mellitus