title: Isoquinoline alkaloids from Coptis japonica stimulate the myoblast differentiation via p38 MAP-kinase and Akt signaling pathway.
abstract: To overcome the muscle atrophy, such as cachexia and sarcopenia, we tried to find myogenic agents from medicinal plants. From myogenic extract of Coptis japonica, we purified six isoquinoline alkaloids and evaluated their effects on transactivation of myoD and MHC expression in C2C12 cells during differentiation process. Among obtained compounds, magnoflorine most efficiently enhanced the myoblast differentiation by activating the p38 MAP kinase and Akt pathway, and also increased the number of multinucleated and cylinder-shaped myotubes. These results propose that magnoflorine from Coptis japonica might be a promising lead compound for the development of anti-muscle atrophy drug.
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea.
authors.forename: Heyjin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea.
authors.forename: Le Thi
authors.initials: LT
authors.lastname: Tuong
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea.
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea.
authors.forename: Gyu-Un
authors.initials: GU
authors.lastname: Bae
authors.affiliation: Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 100 Chungparo 47-Gil, Yongsan-Gu, Seoul 04310, Republic of Korea. Electronic address: ryuha@sookmyung.ac.kr.
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D039323:Coptis / Q000737:chemistry*; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects*
chemical_list: D000470:Alkaloids
keywords: Akt; Coptis japonica; Isoquinoline alkaloid; MyoD; Myoblast differentiation; p38 MAPK