title: Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants.
abstract: Recognition of molecules typical of microbes or aberrant cellular states, termed microbe- or danger-associated molecular patterns (MAMPs/DAMPs), respectively, provides an important step in plant and animal innate immunity. In plants, pattern recognition receptors (PRRs) identified to date are limited to membrane-associated proteins, of which the majority has an extracellular leucine-rich repeat (LRR) or lysine-motif (LysM) domain. These PRRs undergo quality control (QC) in the Endoplasmic Reticulum (ER) that is dependent on Asn (N)-linked glycosylation (Glc3Man9GlcNAc2conjugation) of their extracellular domain. In Arabidopsis, genetic studies have revealed that a subset of these PRRs require an intact N-glycosylation pathway in the ER for their biogenesis and function. Here, we describe methods for immunoblot-based detection of protein glycosylation states in plants.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Takaakira
authors.initials: T
authors.lastname: Inokuchi
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan. saijo@bs.naist.jp.|Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO), Kawaguchi, Japan. saijo@bs.naist.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Saijo
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006031:Glycosylation; D015151:Immunoblotting; D000072417:Protein Domains; D051192:Receptors, Pattern Recognition / Q000032:analysis* / Q000737:chemistry*
chemical_list: D029681:Arabidopsis Proteins; D051192:Receptors, Pattern Recognition
keywords: Endoplasmic Reticulum quality control (ERQC); Glycosidase treatment; Immunoblot analysis; N-linked glycosylation; Pattern recognition receptors (PRRs); Plant immunity