title: ARHGAP1 overexpression inhibits proliferation, migration and invasion of C-33A and SiHa cell lines.
abstract: ARHGAP1, also known asRhoGAP,RhoGAP1,CDC42GAPandp50rhoGAP, is officially named Ras homology (Rho) GTPase-activating protein 1, which is one of the key members of RhoGAPs. Growing evidences demonstrate that several RhoGAPs are suppressed or downregulated in cancers. Thus, the aim of this study was to explore the effects ofARHGAP1on cervical carcinoma cells. The human cervical carcinoma cells C-33A and SiHa were transduced with lentivirus targetingARHGAP1(lenti-ARHGAP1). Cellular proliferation, migration and invasion assays, as well as quantitative real-time polymerase chain reaction and Western blot assays, were performed in the control, negative control (infected with lentivirus) and ARHGAP1+-infected groups. Results showed that overexpression ofARHGAP1markedly inhibited the proliferation of both C-33A and SiHa cells at 24 h, 48 h and 72 h in a time-dependent manner (n=3,P<0.01). Migration and invasion of C-33A and SiHa cells were suppressed after the transduction with lenti-ARHGAP1 compared with the controls (n=3,P<0.01). In addition, several tumor cellular process-related proteins, such as matrix metallopeptidase 2, zinc finger E-box binding homeobox 1, Cyclin B1, twist family bHLH transcription factor 1 and proliferating cell nuclear antigen, were all downregulated inARHGAP1-overexpressed C-33A and SiHa cells and proved to be targets ofARHGAP1. This study indicated that ARHGAP1 may have a positive function on antitumor activity in the treatment of cervical cancer.
authors.affiliation: Department of Gynecology and Obstetrics, Huashan Hospital North.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Li
authors.affiliation: Institute of Antibiotics, Huashan Hospital, Fudan University.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Yin
keywords: ARHGAP1; cervical carcinoma; tumor cellular process-related protein