title: Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events.
abstract: We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (102-1010CFU/mL) and the detection limit (102CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.
authors.affiliation: Department of Chemical Engineering, Kwangwoon University, 20 Gwangun-Ro, Nowon-Gu, Seoul 01897, Republic of Korea.
authors.forename: Min-Sang
authors.initials: MS
authors.lastname: Yoo
authors.affiliation: Department of Chemical Engineering, Kwangwoon University, 20 Gwangun-Ro, Nowon-Gu, Seoul 01897, Republic of Korea.
authors.forename: Minguk
authors.initials: M
authors.lastname: Shin
authors.affiliation: Department of Chemical Engineering, Kwangwoon University, 20 Gwangun-Ro, Nowon-Gu, Seoul 01897, Republic of Korea.
authors.forename: Younghun
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Environmental Engineering, Kwangwoon University, 20 Gwangun-Ro, Nowon-Gu, Seoul 01897, Republic of Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Jang
authors.affiliation: Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul 02841, Republic of Korea.
authors.forename: Yoon-E
authors.initials: YE
authors.lastname: Choi
authors.affiliation: Division of Chemical Engineering and Materials Science, Ewha Womans University, 52 Ewhayeodae-Gil, Seodaemun-Gu, Seoul 03760, Republic of Korea.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.affiliation: School of Integrative Engineering, Chung-Ang University, 84 Heukseok-Ro, Dongjak-Gu, Seoul 06974, Republic of Korea.
authors.forename: Jonghoon
authors.initials: J
authors.lastname: Choi
authors.affiliation: Department of Plant and Food Sciences, Sangmyung University, 31 Sangmyungdae-Gil, Dongnam-Gu, Cheonan-Si, Chungcheongnam-Do 31066, Republic of Korea. Electronic address: dorgly@smu.ac.kr.
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Kwangwoon University, 20 Gwangun-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. Electronic address: chpark@kw.ac.kr.
authors.forename: Chulhwan
authors.initials: C
authors.lastname: Park
mesh_terms: D000391:Air Microbiology; D000393:Air Pollutants / Q000032:analysis*; D001208:Asia; D001412:Bacillus subtilis / Q000302:isolation & purification* / Q000648:ultrastructure; D020562:Betaproteobacteria / Q000302:isolation & purification; D015374:Biosensing Techniques; D004391:Dust / Q000032:analysis*; D055664:Electrochemical Techniques; D004784:Environmental Monitoring; D005417:Flavobacterium / Q000302:isolation & purification; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification
chemical_list: D000393:Air Pollutants; D004391:Dust; D037742:Nanotubes, Carbon
keywords: Asian dust events; Bacillus subtilis; Biosensor; Carbon nanotubes; Pathogen