title: Integrated analysis of recombinant BPV-1 L1 protein for the production of a bovine papillomavirus VLP vaccine.
abstract: Bovine papillomatosis is an infectious disease that is caused by bovine papillomavirus (BPV), which results in important economic losses. However, no BPV vaccines or effective treatment methods are commercially available to date. Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity. The results indicated an absence of genotoxicity of the purified recombinant L1 protein, β-sheet prevalence of secondary structure folding, protein stability under high temperatures as well as the presence of capsomeres and VLPs. In addition, preliminary experimental vaccination of calves showed the production of specific antibodies against BPV-1 L1.
authors.affiliation: Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Diego Grando
authors.initials: DG
authors.lastname: Módolo
authors.affiliation: Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil; Programa de Pós-graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), São Paulo, SP, Brazil.
authors.forename: Rodrigo Pinheiro
authors.initials: RP
authors.lastname: Araldi
authors.affiliation: Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil; Programa de Pós-graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), São Paulo, SP, Brazil.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Mazzuchelli-de-Souza
authors.affiliation: Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Pereira
authors.affiliation: Laboratório de Bioquímica e Biofísica, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Daniel Carvalho
authors.initials: DC
authors.lastname: Pimenta
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil.
authors.forename: Letícia Maria
authors.initials: LM
authors.lastname: Zanphorlin
authors.affiliation: Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Willy
authors.initials: W
authors.lastname: Beçak
authors.affiliation: Instituto de Biologia, Departamento de Genética, Evolução e Bioagentes, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Menossi
authors.affiliation: Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Rita
authors.initials: R
authors.lastname: de Cassia Stocco
authors.affiliation: Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil. Electronic address: rodsfc@uol.com.br.
authors.forename: Rodrigo Franco
authors.initials: RF
authors.lastname: de Carvalho
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D030361:Papillomavirus Infections / Q000517:prevention & control / Q000662:veterinary*; D053918:Papillomavirus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D011487:Protein Conformation; D017510:Protein Folding; D055503:Protein Multimerization; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D058425:Vaccines, Virus-Like Particle / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; C085217:L1 protein, Bovine papillomavirus; D053918:Papillomavirus Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D058425:Vaccines, Virus-Like Particle
keywords: Biosafety; Bovine papillomavirus; Capsomeres; L1 protein; VLP; Vaccine