title: Characterization of miR061 and its target genes in grapevine responding to exogenous gibberellic acid.
abstract: MicroRNAs (miRNAs), as an important growth regulator, are also involved in gibberellic acid (GA) signaling, revealing much relationship between miRNAs and GA in various plant responses. Grape is highly sensitive to GA3, which plays a significant regulatory role in regulation of flower development, berry expansion, berry set, berry ripening, and seedlessness induction; further, it was found that grapevine miR061 (VvmiR061) is a GA3responsive miRNA. In this study, grapevine REV (VvREV) and HOX32 (VvHOX32), two target genes of VvmiR061, were predicted, verified, and cloned; homologous conservation was analyzed in various plants. The expression profiles of both VvmiR061 and its target genes (VvREV and VvHOX32) under GA3treatment were detected by qRT-PCR during grapevine flower and berry development. Results revealed that GA3treatment has upregulated the transcription of VvREV and VvHOX32, while it downregulated the expression of VvmiR061. The function of VvmiR061 in cleaving target genes VvREV and VvHOX32 was diminished by GA3treatment during flower developmental process. The results of this study exhibited the importance of VvmiR061 in regulating flower development and GA3signaling pathway and also contributed some to the knowledge of small RNA-mediated regulation in grape.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.|College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China. Wangchen@njau.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Cui
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Lide
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Chaobo
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China. shangguanlf@njau.edu.cn.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Shangguan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors.forename: Jinggui
authors.initials: J
authors.lastname: Fang
mesh_terms: D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D027843:Vitis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
chemical_list: D005875:Gibberellins; D035683:MicroRNAs; D010937:Plant Growth Regulators; D010940:Plant Proteins; C007842:gibberellic acid
keywords: Gibberellic acid; Target genes; VvREV and VvHOX32; miR061