title: Hybrid Nanoparticle Pyramids for Intracellular Dual MicroRNAs Biosensing and Bioimaging.
abstract: This study strategically fabricates multifunctional nanopyramids to allow the ultrasensitive quantification of dual microRNAs (miR-203band miR-21) in living cells and their responsive bioimaging in vivo. The nanopyramids, composed of Au-Cu9S5nanoparticles (NPs), upconversion NPs (UCNPs), and Ag2S NPs, emit two luminescent signals simultaneously with excitation at 808 nm, arising from the UCNPs at 541 nm in the visible region and from the Ag2S NPs at 1227 nm in the second window of near-infrared (NIR-II) region. The upconversion luminescence has a linear relationship with miR-203bfrom 0.13 to 54.54 fmol per 10 µgRNAand a limit of detection (LOD) of 0.09 fmol per 10 µgRNA, whereas the Ag2S NP luminescence has a linear relationship with miR-21 from 0.37 to 43.56 fmol per 10 µgRNA, with a LOD of 0.23 fmol per 10 µgRNA. Significantly, this study demonstrates that the nanopyramids can be successfully used for miRs-responsive bioimaging in a tumor-bearing animal model. Furthermore, taking advantage of the photothermal capabilities of pyramids, the tumors can also be eliminated completely. These nanopyramids not only overcome the obstacles in the simultaneous detection of multiple miRs at the cellular level but also provide a cancer theranostic platform in vivo.
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.|International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.|International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.|International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors.forename: Maozhong
authors.initials: M
authors.lastname: Sun
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.|International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.|International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.|International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.|International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D042541:Intracellular Space; D057230:Limit of Detection; D049449:Luminescence; D035683:MicroRNAs; D053758:Nanoparticles
chemical_list: D035683:MicroRNAs
keywords: bioimaging; biosensing; hybrid nanoparticle pyramids; intracellular dual microRNAs; tumor ablation