title: Dataset of proteomics analysis of agingC. elegansexposed toPseudomonas aeruginosastrain PA01.
abstract: Here, we present the proteomics dataset of young and middle-agedCaenorhabditis elegans(C. elegans) exposed toPseudomonas aeruginosa(P. aeruginosastrain PA01), which is related to the article "Proteomic Identification of Virulence-Related Factors in Young and AgingC. elegansinfected withPseudomonas aeruginosa" (C. D. King et. al, in-revisions). This dataset was generated to better understand the effects of aging on molecular mechanisms involved in host response to pathogen exposure. Protein fromC. elegansof different age and exposure toP. aeruginosaPA01 or controlE. coliOP50 were extracted and tryptically digested. Peptides were labeled with the reagents tandem mass tag (TMT6-plex), separated, and detected by using offline strong-cation exchange and online liquid chromatography - mass spectrometry (SCX - LC - MS/MS & MS3). A separate mixture of peptides were labeled on N-terminal amines and lysines with dimethylation. Dimethylated peptides were analyzed using LC - MS/MS and a portion of the results were used to verify fold-change direction for TMT6-plex experiments. Raw data can be found online at www.CHORUSproject.org, a cloud-based data repository (see specifications table for details).
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Christina D
authors.initials: CD
authors.lastname: King
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Daljeet
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department of Pediatrics, Rangos Research Center, Children׳s Hospital of Pittsburgh, Pittsburgh, PA 15224, USA.
authors.forename: Kyle
authors.initials: K
authors.lastname: Holden
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Annie B
authors.initials: AB
authors.lastname: Govan
authors.affiliation: Department of Pediatrics, Rangos Research Center, Children׳s Hospital of Pittsburgh, Pittsburgh, PA 15224, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Keith
authors.affiliation: Department of Pediatrics, Rangos Research Center, Children׳s Hospital of Pittsburgh, Pittsburgh, PA 15224, USA.
authors.forename: Arjumand
authors.initials: A
authors.lastname: Ghazi
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Renã A S
authors.initials: RA
authors.lastname: Robinson
keywords: Aging; C.elegans; Immunity; Proteomics; Pseudomonas aeruginosa