title: [Establishment and Application of a Method for Determination of Glycosyltransferase Activity].
abstract: OBJECTIVE
  To establish a method for determination of glycosyltransferase and to explore the enzyme A, B glycosyltransferase activity in human serum so as to lay the foundation for the determination of enzyme level and enzyme activity.
  
  
  METHODS
  The glycosyltransferase activity kit was used to draw phosphate standard curves in our laboratory. The A and B glycosyltransferase activity were determined by the standard curves.
  
  
  RESULTS
  The standard curves (y=2671.3x-0.596 R2=0.9998) for determing glycosyltransferase activity suitable for use in our laboratory were drawn. At the same time the method was set up for determination of A, B glycosyltransferase in human serum.
  
  
  CONCLUSIONS
  The establised method of the determination of glycosyltransferase is suitable for common type of enzyme activity and suitable for the A, B glycosyltransferase in human serum.
authors.affiliation: Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China. E-mail:lianviky@126.com.
authors.forename: Yan-Lian
authors.initials: YL
authors.lastname: Liang
authors.affiliation: Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Su
authors.affiliation: Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
authors.forename: Yin-Ze
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
authors.forename: Da-Cheng
authors.initials: DC
authors.lastname: Li
mesh_terms: D016695:Glycosyltransferases / Q000032:analysis / Q000378:metabolism*; D006801:Humans
chemical_list: D016695:Glycosyltransferases