title: Parasitism drives host genome evolution: Insights from the Pasteuria ramosa-Daphnia magna system.
abstract: Because parasitism is thought to play a major role in shaping host genomes, it has been predicted that genomic regions associated with resistance to parasites should stand out in genome scans, revealing signals of selection above the genomic background. To test whether parasitism is indeed such a major factor in host evolution and to better understand host-parasite interaction at the molecular level, we studied genome-wide polymorphisms in 97 genotypes of the planktonic crustacean Daphnia magna originating from three localities across Europe. Daphnia magna is known to coevolve with the bacterial pathogen Pasteuria ramosa for which host genotypes (clonal lines) are either resistant or susceptible. Using association mapping, we identified two genomic regions involved in resistance to P. ramosa, one of which was already known from a previous QTL analysis. We then performed a naïve genome scan to test for signatures of positive selection and found that the two regions identified with the association mapping further stood out as outliers. Several other regions with evidence for selection were also found, but no link between these regions and phenotypic variation could be established. Our results are consistent with the hypothesis that parasitism is driving host genome evolution.
authors.affiliation: Zoological Institute, Basel University, Vesalgasse 1, 4051, Basel, Switzerland.
authors.forename: Yann
authors.initials: Y
authors.lastname: Bourgeois
authors.affiliation: Zoological Institute, Basel University, Vesalgasse 1, 4051, Basel, Switzerland.|Institute of Plant and Microbial Biology, Zollikerstrasse 107, 8008, Zürich, Switzerland.
authors.forename: Anne C
authors.initials: AC
authors.lastname: Roulin
authors.affiliation: Zoological Institute, Basel University, Vesalgasse 1, 4051, Basel, Switzerland.
authors.forename: Kristina
authors.initials: K
authors.lastname: Müller
authors.affiliation: Zoological Institute, Basel University, Vesalgasse 1, 4051, Basel, Switzerland.
authors.forename: Dieter
authors.initials: D
authors.lastname: Ebert
mesh_terms: D000818:Animals; D003621:Daphnia / Q000235:genetics* / Q000382:microbiology*; D019143:Evolution, Molecular; D016678:Genome; D054884:Host-Pathogen Interactions; D058676:Pasteuria / Q000502:physiology*
keywords: Association mapping; Daphnia magna; Pasteuria ramosa; host-parasite interactions; local adaptation; selection analysis