title: Real-time PCR assay for distinguishing Frankliniella occidentalis and Thrips palmi Arnika Przybylska, Żaneta Fiedler, Aleksandra Obrępalska-Stęplowska.
abstract: Thrips palmi and Frankliniella occidentalis (order Thysanoptera) are thrips species that represent major plant pests. They are polyphagous insects capable of adversely affecting crop production. As such, in the European Union, these thrips species should be regulated as quarantine organisms. T. palmi and F. occidentalis can cause considerable damage to susceptible plants by feeding on them and transmitting several viruses responsible for serious plant diseases. Successful pest control strategies are based on an early, fast, and reliable diagnosis, which precedes the selection of appropriate steps to limit the effects of harmful organisms. We herein describe a novel diagnostic approach that enables the sensitive and species-specific detection (and differentiation) of these pests in a duplex polymerase chain reaction assay, which was adapted for both standard and real-time quantitative assays. Our method is based on the amplification of a 5.8S-internal transcribed spacer 2 ribosomal DNA fragment that is conserved between T. palmi and F. occidentalis.
authors.affiliation: Institute of Plant Protection - National Research Institute,20 Władysława Węgorka St, Poznań,Poland.
authors.forename: Arnika
authors.initials: A
authors.lastname: Przybylska
authors.affiliation: Institute of Plant Protection - National Research Institute,20 Władysława Węgorka St, Poznań,Poland.
authors.forename: Żaneta
authors.initials: Ż
authors.lastname: Fiedler
authors.affiliation: Institute of Plant Protection - National Research Institute,20 Władysława Węgorka St, Poznań,Poland.
authors.forename: Patryk
authors.initials: P
authors.lastname: Frąckowiak
authors.affiliation: Institute of Plant Protection - National Research Institute,20 Władysława Węgorka St, Poznań,Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Obrępalska-Stęplowska
mesh_terms: D000818:Animals; D016133:Polymerase Chain Reaction; D061149:Thysanoptera / Q000145:classification* / Q000235:genetics
keywords: diagnostic protocol; insect detection; molecular methods; multiplex PCR; pest control; quarantine pests; rDNA