title: Exploration of inhibitory mechanisms of curcumin in lung cancer metastasis using a miRNA- transcription factor-target gene network.
abstract: The present study was aimed to unravel the inhibitory mechanisms of curcumin for lung cancer metastasis via constructing a miRNA-transcription factor (TF)-target gene network. Differentially expressed miRNAs between human high-metastatic non-small cell lung cancer 95D cells treated with and without curcumin were identified using a TaqMan human miRNA array followed by real-time PCR, out of which, the top 6 miRNAs (miR-302b-3p, miR-335-5p, miR-338-3p, miR-34c-5p, miR-29c-3p and miR-34a-35p) with more verified target genes and TFs than other miRNAs as confirmed by a literature review were selected for further analysis. The miRecords database was utilized to predict the target genes of these 6 miRNAs, TFs of which were identified based on the TRANSFAC database. The findings of the above procedure were used to construct a miRNA-TF-target gene network, among which miR-34a-5p, miR-34c-5p and miR-302b-3p seemed to regulate CCND1, WNT1 and MYC to be involved in Wnt signaling pathway through the LEF1 transcription factor. Therefore, we suggest miR-34a-5p/miR-34c-5p/miR-302b-3p -LEF1-CCND1/WNT1/MYC axis may be a crucial mechanism in inhibition of lung cancer metastasis by curcumin.
authors.affiliation: Department of Respiratory Disease, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: De-Min
authors.initials: DM
authors.lastname: Jiao
authors.affiliation: Department of Oncology, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, P.R. China.
authors.forename: Li-Shan
authors.initials: LS
authors.lastname: Wang
authors.affiliation: Department of Respiratory Disease, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: Hui-Zhen
authors.initials: HZ
authors.lastname: Hu
authors.affiliation: Department of Respiratory Disease, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: Xia-Li
authors.initials: XL
authors.lastname: Tang
authors.affiliation: Department of Respiratory Disease, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Respiratory Disease, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Respiratory Disease, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: You
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Respiratory Disease, The 117th Hospital of PLA, Hangzhou, Zhejiang, P.R. China.
authors.forename: Qing-Yong
authors.initials: QY
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000970:Antineoplastic Agents; D035683:MicroRNAs; D003474:Curcumin