title: Anti-inflammatory evaluation and acute toxicity of three food supplements that contain Moussonia deppeana.
abstract: OBJECTIVE
  To identify the anti-inflammatory activity through two murine models and in the median Lethal Dose (LD50) of three dietary supplements that contain Moussonia deppeana.
  
  
  METHODS
  The anti-inflammatory activity of three dietary supplements (Cicatrisan/Gastricus®, Gastinol®, and Gastrovita®) EtOH extracts was evaluated by TPA and by carrageenan murine models; also, median Lethal Dose (LD50) was determined. Verbascoside was quantified by High-Performance Liquid Chromatography. β-sitosterol, stigmasterol and the mixture of ursolic and oleanolic acids were identified in all supplements by TLC; however, none of these dietary supplements contain verbascoside.
  
  
  RESULTS
  For the TPA model, Cicatrisan/Gastricus®generated a notable effect with 38.24% inhibition. While in the carrageenan model, it also exhibited noteworthy anti-inflammatory activity of ear edema with 66.39% of paw edema inhibition at 150 mg/kg, followed by Gastinol®and Gastrovita®with ≈50% at 300 mg/kg. Finally, LD50was >2 g/kg for all supplements, when was administered intragastrically and Body Weight (BW) gain in mice was not altered after 14 days.
  
  
  CONCLUSIONS
  Of the three food supplements containing M. deppeana, only the EtOH extract from Cicatrisan/Gastricus®formulation (tablets) showed significant anti-inflammatory activity in both experimental models and the LD50was >2 g/kg.
authors.affiliation: Unidad de Investigación Médica en Farmacología, Hospital de Especialidades, Centro Médico Nacional Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS), Av. Cuauhtémoc 330, Col. Doctores, Deleg. Cuauhtémoc, 06720, Ciudad de México (CDMX), Mexico.
authors.forename: Gabriel Alfonso
authors.initials: GA
authors.lastname: Gutiérrez-Rebolledo
authors.affiliation: Unidad de Investigación Médica en Farmacología, Hospital de Especialidades, Centro Médico Nacional Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS), Av. Cuauhtémoc 330, Col. Doctores, Deleg. Cuauhtémoc, 06720, Ciudad de México (CDMX), Mexico.
authors.forename: Mariana Zuleima
authors.initials: MZ
authors.lastname: Pérez-González
authors.affiliation: Centro de Investigación Biomédica del Sur (CIBIS), IMSS, Argentina No. 1, Col. Centro, 62790, Xochitepec, Morelos, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zamilpa
authors.affiliation: Unidad de Investigación Médica en Farmacología, Hospital de Especialidades, Centro Médico Nacional Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS), Av. Cuauhtémoc 330, Col. Doctores, Deleg. Cuauhtémoc, 06720, Ciudad de México (CDMX), Mexico. Electronic address: adelinajim08@prodigy.net.mx.
authors.forename: María Adelina
authors.initials: MA
authors.lastname: Jiménez-Arellanes
keywords: Acute inflammation; Food supplements; Herbal remedies; Mexican medicinal plants; Moussonia deppeana; Traditional medicine