title: Important role of collective cell migration and nerve fiber density in the development of deep nodular endometriosis.
abstract: To evaluate deep nodular endometriotic lesions induced in baboons over 12 months and analyze collective cell migration and nerve fiber density.
  
  
  
  Morphologic and immunohistochemical analysis of endometriotic lesions induced in baboons over the course of 1 year.
  
  
  
  Academic research unit.
  
  
  
  Three female baboons (Papio anubis).
  
  
  
  Recovery of induced deep nodular endometriotic nodules from baboons.
  
  
  
  Evaluation of the morphology of glands by analysis of the center of lesions and the invasion front; immunohistochemical staining with Ki67, E-cadherin, and β-catenin for investigation of mitotic activity and cell-cell junctions, and with protein gene product 9.5 and nerve growth factor (NGF) for study of nerve fiber density (NFD).
  
  
  
  All (100%) of the lesions were invasive 1 year after induction, compared with 42.29% after 6 months. Glands from the invasion front showed significantly reduced thickness but significantly higher mitotic activity. E-Cadherin and β-catenin expression were similar between the center and front. NFD was significantly higher in lesions induced after 1 year than after 6 months, and NGF expression was significantly lower in 1-year lesions than in 6-month lesions.
  
  
  
  Nodular endometriotic lesions induced in the baboon model were found to be significantly more invasive and innervated after 12 months than after 6 months. The invasive phenotype was highly expressed in glands at the invasion front, and our study suggests that nerve fibers play a role in the development of lesions as observed in women.
authors.affiliation: Pôle de Gynécologie, Institut de Recherche Expérimentale, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Renan
authors.initials: R
authors.lastname: Orellana
authors.affiliation: Pôle de Gynécologie, Institut de Recherche Expérimentale, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Javier
authors.initials: J
authors.lastname: García-Solares
authors.affiliation: Société de Recherche pour l'Infertilité, Brussels, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Donnez
authors.affiliation: Pôle de Gynécologie, Institut de Recherche Expérimentale, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Olivier
authors.initials: O
authors.lastname: van Kerk
authors.affiliation: Pôle de Gynécologie, Institut de Recherche Expérimentale, Université Catholique de Louvain, Brussels, Belgium; Gynecology Department, Cliniques Universitaires Saint Luc, Brussels, Belgium. Electronic address: marie-madeleine.dolmans@uclouvain.be.
authors.forename: Marie-Madeleine
authors.initials: MM
authors.lastname: Dolmans
authors.affiliation: Institut du Sein et de Chirurgie Gynécologique d'Avignon, Polyclinique Urbain V (ELSAN Group), Avignon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Donnez
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement; D004195:Disease Models, Animal; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000294:innervation / Q000378:metabolism / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008938:Mitosis; D009412:Nerve Fibers / Q000378:metabolism / Q000473:pathology*; D020932:Nerve Growth Factor / Q000378:metabolism; D048530:Papio anubis; D010641:Phenotype; D013997:Time Factors; D043222:Ubiquitin Thiolesterase / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D015820:Cadherins; D019394:Ki-67 Antigen; D051176:beta Catenin; D020932:Nerve Growth Factor; D043222:Ubiquitin Thiolesterase
keywords: Deep nodular endometriosis; baboon model; collective cell migration; invasion; nerve fiber density; nerve fibers