title: Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle.
abstract: Skeletal muscle stem cell (MuSC) isolation and transplantation are invaluable tools to assess their capacity for self-renewal and tissue repair. Significant technical advances in recent years have led to the optimization of these approaches, improving our ability to assess MuSC regenerative potential. Here, we describe the procedures for Fluorescent Activated Cell Sorting (FACS)-based isolation of MuSC, their intramuscular transplantation, and analysis of their engraftment into host tissues.
authors.affiliation: Graduate School of Biomedical Sciences, Sanford-Burnham-Prebys Medical Discovery Institute, 10901 N Torrey Pines Road, La Jolla, CA, 92037, USA.|Development, Aging and Regeneration Program, Sanford Children's Health Research Center, Sanford-Burnham-Prebys Medical Discovery Institute, 10901 N Torrey Pines Rd, La Jolla, CA, 92037, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Tierney
authors.affiliation: Development, Aging and Regeneration Program, Sanford Children's Health Research Center, Sanford-Burnham-Prebys Medical Discovery Institute, 10901 N Torrey Pines Rd, La Jolla, CA, 92037, USA. asacco@sbpdiscovery.org.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sacco
mesh_terms: D000818:Animals; D015415:Biomarkers; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D016130:Immunophenotyping / Q000379:methods; D051379:Mice; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000166:cytology*; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014945:Wound Healing
chemical_list: D015415:Biomarkers
keywords: Engraftment; FACS-based cell isolation; Muscle regeneration; Skeletal muscle stem cells; Transplantation