title: Downregulation of a novel long noncoding RNA TRPM2-AS promotes apoptosis in non-small cell lung cancer.
abstract: Non-small cell lung cancer is one of the most common types of cancer, and the prognosis of non-small cell lung cancer is still poor. Recent evidence has proved that long noncoding RNA is involved in tumorigenesis. For non-small cell lung cancer, the expression profile of long noncoding RNA has been studied. Here, we identified a novel long noncoding RNA TRPM2-AS from published dataset and found TRPM2-AS is widely upregulated in non-small cell lung cancer tissues compared with adjacent non-tumor tissues. Higher expression level of TRPM2-AS was correlated with higher TNM stages and larger tumor size. Patients with high TRPM2-AS expression level had poor survival than those with low TRPM2-AS level. We silenced TRPM2-AS by small interfering RNA and found that cell proliferation was significantly inhibited after knockdown of TRPM2-AS. Annexin V/propidium iodide staining and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay confirmed that cell apoptosis increased after TRPM2-AS knockdown. Further experiments showed that silence of TRPM2-AS upregulated SHC1 and silence of SHC1 partially reversed cell apoptosis after TRPM2-AS knockdown. In summary, the novel long noncoding RNA TRPM2-AS upregulated in non-small cell lung cancer, and downregulation of TRPM2-AS promotes apoptosis in vitro.
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Yingzhi
authors.initials: Y
authors.lastname: Qin
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Liu
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Naixin
authors.initials: N
authors.lastname: Liang
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Yeye
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Ma
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Han
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.affiliation: 1 Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.|2 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Shanqing
authors.initials: S
authors.lastname: Li
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis / Q000502:physiology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1 / Q000096:biosynthesis / Q000235:genetics; D050053:TRPM Cation Channels / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation
chemical_list: C528024:SHC1 protein, human; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D050053:TRPM Cation Channels; C121934:TRPM2 protein, human
keywords: Long noncoding RNA; TRPM2-AS; apoptosis; non–small cell lung cancer