title: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion.
abstract: Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zong
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Kunling
authors.initials: K
authors.lastname: Chen
authors.affiliation: Genovo Biotechnology Co., Ltd, Tianjin, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Ran
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Gao
mesh_terms: D020029:Base Pairing / Q000235:genetics; D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D003564:Cytidine Deaminase / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D017343:Genes, Plant / Q000235:genetics; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D012275:Oryza / Q000235:genetics; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D017354:Point Mutation / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014908:Triticum / Q000235:genetics; D003313:Zea mays / Q000235:genetics
chemical_list: D064130:CRISPR-Associated Proteins; D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D003564:Cytidine Deaminase