title: Induced circularly polarized luminescence for revealing DNA binding with fluorescent dyes.
abstract: To the best of our knowledge this is the first report on the application of induced circularly polarized luminescence (CPL) for sensing the binding of fluorescent dyes to double stranded DNA. Using Thiazole Orange (TO) and 4',6-diamidino-2-phenylindole (DAPI) as models, we show utility and limitations of CPL in DNA binding studies. The results obtained indicate that CPL can be used as a new chiroptical tool for this purpose, however, special attention while recording CPL data must be used, in order to exclude measurement artefacts caused by linear polarization components.
authors.affiliation: Dipartimento di Chimica e Chimica Industriale, Università di Pisa, via G. Moruzzi 13, 56124 Pisa, Italy.
authors.forename: Marcin
authors.initials: M
authors.lastname: Górecki
authors.affiliation: Dipartimento di Chimica e Chimica Industriale, Università di Pisa, via G. Moruzzi 13, 56124 Pisa, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Zinna
authors.affiliation: Dipartimento di Chimica e Chimica Industriale, Università di Pisa, via G. Moruzzi 13, 56124 Pisa, Italy.
authors.forename: Tarita
authors.initials: T
authors.lastname: Biver
authors.affiliation: Dipartimento di Chimica e Chimica Industriale, Università di Pisa, via G. Moruzzi 13, 56124 Pisa, Italy. Electronic address: lorenzo.dibari@unipi.it.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Di Bari
mesh_terms: D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D049449:Luminescence
chemical_list: D005456:Fluorescent Dyes; D004247:DNA