title: Physico-chemical characterization and antibacterial activity of inclusion complexes of Hyptis martiusii Benth essential oil in β-cyclodextrin.
abstract: Cyclodextrins (CDs) have been used as important pharmaceutical excipients for improve the physicochemical properties of the drugs of low solubility as the essential oil of Hyptis martiusii. This oil is important therapeutically, but the low solubility and bioavailability compromises your use. Therein, the aim of this study was to obtain and to characterize physico-chemically the samples obtained by physical mixture (PM), paste complexation (PC) and slurry complexation (SC) of the essential oil Hyptis martiusii (EOHM) in β-CD, and to compare the antibacterial and modulatory-antibiotic activity of products obtained and oil free. The physicochemical characterization was performed by differential scanning calorimetry (DSC), thermogravimetry/derivative thermogravimetry (TG/DTG), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Karl Fischer titration. Additionally, the antibacterial tests were performed by microdilution technique. Thus, it was observed that the PM method showed low complexing capacity, unlike PC and SC in which it was observed the formation of inclusion complexes. In addition, the second stage of the TG/DTG curves showed that SC was the best method inclusion with mass loss of 6.9% over the PC that was 6.0%. The XRD results corroborate with the results above suggesting the formation of new solid phase and the SEM photomicrographs showed the porous surface of the samples PC and SC. The essential oil alone demonstrated an antibacterial and modulatory effect against the S. aureus and the Gram negative strain, respectively. However, the β-CD and the inclusion complex did not demonstrate any biological activity in the performed antibacterial assays.
authors.affiliation: Pharmacy Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Tatianny A
authors.initials: TA
authors.lastname: Andrade
authors.affiliation: Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
authors.forename: Thiago S
authors.initials: TS
authors.lastname: Freitas
authors.affiliation: Pharmacy Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Francielly O
authors.initials: FO
authors.lastname: Araújo
authors.affiliation: Pharmacy Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Paula P
authors.initials: PP
authors.lastname: Menezes
authors.affiliation: Pharmacy Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Grace Anne A
authors.initials: GAA
authors.lastname: Dória
authors.affiliation: Pharmacy Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Alessandra S
authors.initials: AS
authors.lastname: Rabelo
authors.affiliation: Physiology Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Lucindo J
authors.initials: LJ
authors.lastname: Quintans-Júnior
authors.affiliation: Physiology Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Márcio R V
authors.initials: MRV
authors.lastname: Santos
authors.affiliation: Gonçalo Muniz Research Center, Oswaldo Cruz Foundation, Bahia, Brazil.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Bezerra
authors.affiliation: Pharmacy Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Mairim R
authors.initials: MR
authors.lastname: Serafini
authors.affiliation: Department of Mineralogy and Geotectonics, Institute of Geosciences, University of SãoPaulo-SP, Brazil.
authors.forename: Irwin Rose A
authors.initials: IRA
authors.lastname: Menezes
authors.affiliation: Physiology Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Paula Santos
authors.initials: PS
authors.lastname: Nunes
authors.affiliation: Pharmacy Department, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Adriano A S
authors.initials: AAS
authors.lastname: Araújo
authors.affiliation: Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
authors.forename: Maria S
authors.initials: MS
authors.lastname: Costa
authors.affiliation: Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
authors.forename: Fábia F
authors.initials: FF
authors.lastname: Campina
authors.affiliation: Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
authors.forename: Antonia T L
authors.initials: ATL
authors.lastname: Santos
authors.affiliation: Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
authors.forename: Ana R P
authors.initials: ARP
authors.lastname: Silva
authors.affiliation: Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil. Electronic address: hdmcoutinho@gmail.com.
authors.forename: Henrique D M
authors.initials: HDM
authors.lastname: Coutinho
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology; D004339:Drug Compounding / Q000379:methods; D006090:Gram-Negative Bacteria / Q000187:drug effects; D031331:Hyptis / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction / Q000379:methods; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003505:Cyclodextrins; D009822:Oils, Volatile; D010936:Plant Extracts; D047392:beta-Cyclodextrins
keywords: Antibacterial activity; Essential oil; Hyptis martiusii; Inclusion complex; Modulatory-antibiotic activity; β-CD