title: De Novo Transcriptome Analysis of Plant Pathogenic Fungus Myrothecium roridum and Identification of Genes Associated with Trichothecene Mycotoxin Biosynthesis.
abstract: Myrothecium roridumis a plant pathogenic fungus that infects different crops and decreases the yield of economical crops, including soybean, cotton, corn, pepper, and tomato. Until now, the pathogenic mechanism ofM. roridumhas remained unclear. Different types of trichothecene mycotoxins were isolated fromM. roridum, and trichothecene was considered as a plant pathogenic factor ofM. roridum. In this study, the transcriptome ofM. roridumin different incubation durations was sequenced using an Illumina Hiseq 2000. A total of 35,485 transcripts and 25,996 unigenes forM. roridumwere obtained from 8.0 Gb clean reads. The protein-protein network of theM. roridumtranscriptome indicated that the mitogen-activated protein kinases signal pathway also played an important role in the pathogenicity ofM. roridum.The genes related to trichothecene biosynthesis were annotated. The expression levels of these genes were also predicted and validated through quantitative real-time polymerase chain reaction.Tri5gene encoding trichodiene synthase was cloned and expressed, and the purified trichodiene synthase was able to catalyze farnesyl pyrophosphate into different kinds of sesquiterpenoids.Tri4andTri11genes were expressed inEscherichia coli, and their corresponding enzymatic properties were characterized. The phylogenetic tree of trichodiene synthase showed a great discrepancy between the trichodiene synthase fromM. roridumand other species. Our study on the genes related to trichothecene biosynthesis establishes a foundation for theM. roridumhazard prevention, thus improving the yields of economical crops.
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, China. yewei@gdim.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, China. ltm840801@163.com.
authors.forename: Taomei
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, China. zhumuzi@foxmail.com.
authors.forename: Muzi
authors.initials: M
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, China. wmzhang@gdim.cn.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, China. Lihh@gdim.cn.
authors.forename: Haohua
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, China. yaoyz@gdim.cn.
authors.forename: Zilei
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou 510070, China. lisn@gdim.cn.
authors.forename: Saini
authors.initials: S
authors.lastname: Li
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways; D002384:Catalysis; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D018895:Microsatellite Repeats; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D058977:Molecular Sequence Annotation; D009183:Mycotoxins / Q000096:biosynthesis*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D015398:Signal Transduction; D059467:Transcriptome; D014255:Trichothecenes / Q000096:biosynthesis*
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; D020928:Mitogen-Activated Protein Kinases
keywords: MAPK signal pathway; Myrothecium roridum; plant pathogenic fungus; transcriptome; trichothecene biosynthesis