title: Prognostic significance of long noncoding RNA Z38 as a candidate biomarker in breast cancer.
abstract: BACKGROUND
  Long noncoding RNA (lncRNA) Z38 has been shown to promote cell proliferation and tumorigenesis in breast cancer. However, expression pattern and prognostic value of lncRNA Z38 in breast cancer patients remain elusive.
  
  
  METHODS
  The expression levels of SPRY4-IT1 in 110 self-paired specimens of breast cancer and adjacent normal breast tissues were measured by quantitative real-time PCR (qRT-PCR), and its correlation with overall survival of patients with breast cancer was further statistically analyzed.
  
  
  RESULTS
  Compared with normal breast tissues, Z38 was upregulated in breast cancer tissues. Furthermore, of 110 breast cancer patients, high Z38 expression was significantly associated with tumor-node-metastasis stage and lymph node metastasis. Further analysis using the Cox regression model revealed that Z38 expression was an independent prognostic factor of overall survival in patients with breast cancer (hazard ratio=4.74, 95% confidence interval 2.41-9.32). The nomogram presents a good prediction of the probability of overall survival of breast cancer patients (c-index: 0.792), and its predictive efficiency was further confirmed by the calibration curve.
  
  
  CONCLUSIONS
  Our data highlighted the potential of lncRNA Z38 as novel candidate biomarker to identify patients with breast cancer at high risk of tumor death.
authors.affiliation: Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Nie
authors.affiliation: Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yi-Shan
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Mei
authors.affiliation: Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Zhang
authors.affiliation: General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Sun
authors.affiliation: Department of Cardiology, The Second People's Hospital of Lianyungang, Lianyungang, China.
authors.forename: Yi-Lian
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Xia
authors.affiliation: General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Shu-Kui
authors.identifier: http://orcid.org/0000-0001-6972-2587
authors.initials: SK
authors.lastname: Wang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding
keywords: Z38; breast cancer; long noncoding RNA; prognosis