title: Upregulated expression of La ribonucleoprotein domain family member 6 and collagen type I gene following water-filtered broad-spectrum near-infrared irradiation in a 3-dimensional human epidermal tissue culture model as revealed by microarray analysis.
abstract: OBJECTIVE
  Water-filtered broad-spectrum near-infrared irradiation can induce various biological effects, as our previous clinical, histological, and biochemical investigations have shown. However, few studies that examined the changes thus induced in gene expression. The aim was to investigate the changes in gene expression in a 3-dimensional reconstructed epidermal tissue culture exposed to water-filtered broad-spectrum near-infrared irradiation.
  
  
  METHODS
  DNA microarray and quantitative real-time polymerase chain reaction (PCR) analysis was used to assess gene expression levels in a 3-dimensional reconstructed epidermal model composed of normal human epidermal cells exposed to water-filtered broad-spectrum near-infrared irradiation. The water filter allowed 1000-1800 nm wavelengths and excluded 1400-1500 nm wavelengths, and cells were exposed to 5 or 10 rounds of near-infrared irradiation at 10 J/cm2.
  
  
  RESULTS
  A DNA microarray with over 50 000 different probes showed 18 genes that were upregulated or downregulated by at least twofold after irradiation. Quantitative real-time PCR revealed that, relative to control cells, the gene encoding La ribonucleoprotein domain family member 6 (LARP6), which regulates collagen expression, was significantly and dose-dependently upregulated (P < 0.05) by water-filtered broad-spectrum near-infrared exposure. Gene encoding transcripts of collagen type I were significantly upregulated compared with controls (P < 0.05).
  
  
  CONCLUSIONS
  This study demonstrates the ability of water-filtered broad-spectrum near-infrared irradiation to stimulate the production of type I collagen.
authors.affiliation: Clinica Tanaka Plastic, Reconstructive Surgery and Anti-aging Center, Matsumoto, Nagano, Japan.|Department of Molecular Pathology, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Molecular Pathology, Shinshu University Graduate School of Medicine, Matsumoto, Nagano, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Nakayama
mesh_terms: D001324:Autoantigens / Q000235:genetics*; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D004307:Dose-Response Relationship, Radiation; D004817:Epidermis / Q000528:radiation effects*; D006801:Humans; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis; D012261:Ribonucleoproteins / Q000235:genetics*; D046509:Tissue Culture Techniques; D015854:Up-Regulation / Q000528:radiation effects*; D014867:Water; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D012261:Ribonucleoproteins; D014867:Water; D000097585:SS-B Antigen
keywords: 
  COL1A1
  ; 
  LARP6
  ; 3-dimensional human epidermal tissue culture model; collagen synthesis; water-filtered broad-spectrum near-infrared