title: The effects of venous ensheathment on facial nerve repair in the rat.
abstract: To investigate the protective effect of autologous venous ensheathment on sutured rat facial nerve and to test whether the ensheathment could improve the functional recovery of repaired nerve and accuracy of axonal growth.
  
  
  
  In vivo study.
  
  
  
  Forty-six rats were examined, with six rats serving as normal controls and 40 receiving facial nerve transection and suture repair (SR) or transection and suture repair with an additional venous ensheathment (VE). The rats were then subjected to functional testing, histological assessment of nerve specimens, or retrograde tracing, respectively.
  
  
  
  At the postoperative day (POD) 60, the venous ensheathment showed no adhesion at the surrounding tissues. No significant difference in neuroma formation was found between the two surgical manipulations (SR and VE groups) (P < 0.05). Retrogradely labeled motoneurons in facial nuclei were extremely disorganized after the facial nerve undertook surgical manipulation. In all manipulated groups, double retrogradely labeled neurons, indicative of aberrant axonal branching during regeneration, could be observed after peripheral manipulation across all time points. With the two facial surgical manipulations, the average count of double-labeled neurons at POD 60 was significantly less than at POD 21 (P < 0.05).
  
  
  
  Autologous venous ensheathment could not help with the functional recovery of facial nerve or improve the accuracy of axonal regeneration. Further studies are warranted to elucidate the effects of venous ensheathment in other motor and sensory nerve models.
  
  
  
  NA. Laryngoscope, 127:1558-1564, 2017.
authors.affiliation: Department of Otolaryngology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, Massachusetts, U.S.A.|Department of Otolaryngology, Wuhan Integrated TCM and Western Medicine Hospital (Wuhan No.1 Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, Massachusetts, U.S.A.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Knox
authors.affiliation: Department of Otolaryngology, Wuhan Integrated TCM and Western Medicine Hospital (Wuhan No.1 Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Linli
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Otolaryngology, Wuhan Integrated TCM and Western Medicine Hospital (Wuhan No.1 Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, Massachusetts, U.S.A.
authors.forename: Tessa A
authors.initials: TA
authors.lastname: Hadlock
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D001369:Axons / Q000502:physiology*; D004195:Disease Models, Animal; D005154:Facial Nerve / Q000503:physiopathology* / Q000601:surgery*; D020220:Facial Nerve Injuries / Q000503:physiopathology* / Q000601:surgery*; D005260:Female; D008866:Microsurgery / Q000379:methods*; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000473:pathology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014680:Veins / Q000637:transplantation*; D014738:Vibrissae / Q000294:innervation
keywords: Facial nerve; ensheathment; suture techniques; veins