title: [Salidroside protects PC12 cells from H2O2-induced apoptosis via suppressing NOX2-ROS-MAPKs signaling pathway].
abstract: OBJECTIVE
  To investigate the molecular mechanism by which salidroside protects PC12 cells from H2O2-induced apoptosis.
  
  
  METHODS
  PC12 cells cultured in DMEM supplemented with 10% horse serum and 5% fetal bovine serum were pretreated with different doses of salidroside for 2 h and then stimulated with H2O2for different lengths of time. The expression levels of PARP and caspase 3 and the phosphorylation of p38, ERK and JNK were determined with Western blotting. The cell nuclear morphology was observed after DAPI staining. The production of ROS was detected using a ROS detection kit, and the levels of gp91phoxand p47phoxin the membrane and cytoplasm were detected by membrane-cytoplasm separation experiment; the binding between gp91phoxand p47phoxwas assayed by coimmunoprecipitation experiment.
  
  
  RESULTS
  Salidroside dose-dependently suppressed cell apoptosis, lowered phosphorylation levels of p38, ERK and JNK, inhibited the production of ROS, reduced the binding between gp91phoxand p47phox, and inhibited the activity of NOX2 in PC12 cells exposed to H2O2.
  
  
  CONCLUSIONS
  Salidroside protects PC12 cells from H2O2-induced apoptosis at least partly by suppressing NOX2-ROS-MAPKs signaling pathway.
authors.affiliation: Department of Biochemistry1,Wannan Medical College, Wuhu 241002, China; 3Anhui Provincial Key Laboratory of Active Biological Macro-molecules, Wuhu 241002, China. E-mail: 422627721@qq.com.
authors.forename: Zhi-Lin
authors.initials: ZL
authors.lastname: Qi
authors.forename: Yin-Hua
authors.initials: YH
authors.lastname: Liu
authors.forename: Shi-Mei
authors.initials: SM
authors.lastname: Qi
authors.forename: Lie-Feng
authors.initials: LF
authors.lastname: Ling
authors.forename: Zun-Yong
authors.initials: ZY
authors.lastname: Feng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D016716:PC12 Cells; D010636:Phenols / Q000494:pharmacology*; D010766:Phosphorylation; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D005960:Glucosides; D008562:Membrane Glycoproteins; D018696:Neuroprotective Agents; D010636:Phenols; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; C500593:Cybb protein, rat; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D053148:Caspase 3; C009172:rhodioloside