title: Effect of environmentally-relevant concentrations of nonylphenol on sexual differentiation in zebrafish: a multi-generational study.
abstract: Nonylphenol (NP) is a persistent environmental chemical that can disrupt the organism's endocrine system, and is detected in the surface water and sea. In this study, we investigated whether NP can alter transcriptional expression of sexual differentiation-related genes. Three generations of zebrafish were exposed to 0, 2, 20 and 200 μg·L-1of NP, and transcriptional expression of sexual differentiation genes were assessed in 10, 20 and 40 dpf in the F1 and F2 generations. Growth of zebrafish exposed to 200 μg·L-1of NP was inhibited at 125 dpf in the F1 generation. 20 μg·L-1of NP resulted in 80% females in the F1 generation, but had no effect on the F2 generation. In terms of the sexual differentiation genes, the transcriptional expression of cyp19a1a and esr1 genes were upregulated in 20 μg·L-1of NP in the F1 generation. But expression of the sexual differentiation genes were not affected in the F2 generation. Overall, NP could affect sexual differentiation and gene transcriptional expression in the F1 generation. The tolerance of contaminant in the offsprings was improved at low concentration.
authors.affiliation: Research Center of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.affiliation: Research Center of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Research Center of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Ning
authors.initials: N
authors.lastname: He
authors.affiliation: Research Center of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Diao
authors.affiliation: Research Center of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Jia
authors.affiliation: Research Center of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Shun-Shan
authors.initials: SS
authors.lastname: Duan
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D049628:Body Size / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D004781:Environmental Exposure; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005260:Female; D008297:Male; D010636:Phenols / Q000633:toxicity*; D012733:Sex Differentiation / Q000187:drug effects* / Q000235:genetics; D012744:Sex Ratio; D015854:Up-Regulation / Q000187:drug effects; D015027:Zebrafish / Q000254:growth & development / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D010636:Phenols; D029961:Zebrafish Proteins; C000594789:estrogen receptor 1, zebrafish; C025256:nonylphenol; D001141:Aromatase; C440729:Cyp19a1a protein, zebrafish