title: Human cytomegalovirus detection in gastric cancer and its possible association with lymphatic metastasis.
abstract: Increasing evidence suggests that human cytomegalovirus (HCMV) is associated with many human malignancies. However, its prevalence in gastric cancer (GC) and clinical association remain unknown. HCMV IgG and IgM antibodies in the sera of 80 GC patients and 80 healthy controls were detected using a microparticle enzyme immunoassay. The prevalence of HCMV UL47, UL55, UL56, and UL77 genes among 102 GC tumor tissues and adjacent normal specimens was measured by polymerase chain reaction (PCR) or nested PCR. Quantitative real-time PCR (Q-PCR) was used to determine viral load. Virus localization in neoplastic tissues was determined by immunohistochemistry. No significant difference of HCMV IgG and IgM seropositivity was found between GC patients and the healthy group. However, the overall HCMV DNA positivity rate was significantly higher in GC cancerous tissue compared with in paired normal tissue (P<0.01). HCMV infection was mainly localized in the tumorous epithelium. Q-PCR in HCMV-positive specimens indicated that the viral copy number was notably higher in GC tissues than in adjacent normal specimens (P<0.001). Clinical statistical analysis indicated that HCMV load in GC tumor tissue was positively associated with lymphatic metastasis (P=0.043), the area under the receiver operating characteristic (ROC) curve was 0.6638. Our data clearly provide the prevalence of HCMV in GC patients. We conclude that HCMV infection in malignant tissues might be associated with carcinogenesis or progression of GC and possibly relates to lymphatic metastasis.
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of tropical medicine, Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Gangqiang
authors.initials: G
authors.lastname: Guo
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Jinji
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Changyuan
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
authors.forename: Peichen
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China; Department of Gastrointestinal Surgery, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Luchen District, 325027, Wenzhou, Zhe Jiang, P.R.China. Electronic address: 13968888872@163.com.
authors.forename: Xian
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of tropical medicine, Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China. Electronic address: wzxxy001@163.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Xue
mesh_terms: D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D001706:Biopsy; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005814:Genes, Viral; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D057089:Pathology, Molecular; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D013274:Stomach Neoplasms / Q000209:etiology* / Q000473:pathology / Q000821:virology*; D019562:Viral Load
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: Immunohistochemistry; Metastasis; Quantitative real-time PCR; Virus and cancer