title: An in vitro investigation of the marked impact of dendritic cell interactions with bone grafts.
abstract: The immune response to bone biomaterials plays a critical role during early inflammation and biomaterial-induced osteogenesis. Dendritic cells (DCs) possess a significant impact during immune responses to biomaterials. While conventional strategies to evaluate the osteogenic potential of bone substitute materials focus mainly on osteoblast differentiation. Therefore the present study was to investigate the interactions DCs to porous β-tricalcium phosphate (β-TCP) to investigate whether DCs participate in the material-coordinated osteogenic process. DC maturation and function were investigated by detecting the expression of cell surface markers and inflammatory cytokines by RT-PCR. DCs seeded on β-TCP showed a decrease in co-stimulatory molecules (CD86 and CD40) and pro-inflammatory gene IFN-γ but an increase in anti-inflammatory genes including IL-1ra, IL-4, and IL-10, as well as TGF-β1. Thereafter, conditioned media was harvested from DCs seeded on standard tissue culture plastic and β-TCP to be utilized with osteoblast cell cultures. It was found that conditioned media from DCs seeded on β-TCP markedly promoted osteoblast differentiation by increasing the expression of osteogenic genes RUNX2, ALP, OCN as well as increased ALP activity and alizarin red staining in vitro. In conclusion, the findings from the present study point to the potential crucial role of DCs in biomaterial-induced osteogenesis. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1703-1711, 2017.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.|Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.|Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ke
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.|Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.|Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Cell Therapy Institute, Centre for Collaborative Research, Nova Southeastern University, Fort Lauderdale, FL, 33328, USA.|Department of Periodontology, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL, 33328, USA.|Department of Periodontics and Oral Surgery, University of Ann Arbor, Ann Arbor, MI, 48109, USA.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Miron
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.|Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002450:Cell Communication / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000032:analysis; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D017077:Culture Media, Conditioned; D016207:Cytokines; C485817:beta-tricalcium phosphate
keywords: bone substitute materials; dendritic cell; immune response; osteogenesis; β-TCP