title: Exploring the transcriptome of non-model oleaginous microalga Dunaliella tertiolecta through high-throughput sequencing and high performance computing.
abstract: BACKGROUND
  RNA-Seq technology has received a lot of attention in recent years for microalgal global transcriptomic profiling. It is widely used in transcriptome-wide analysis of gene expression., particularly for microalgal strains with potential as biofuel sources. However, insufficient genomic or transcriptomic information of non-model microalgae has limited the understanding of their regulatory mechanisms and hampered genetic manipulation to enhance biofuel production. As such, an optimal microalgal transcriptomic database construction is a subject of urgent investigation.
  
  
  RESULTS
  Dunaliella tertiolecta, a non-model oleaginous microalgal species, was sequenced via Illumina MISEQ and HISEQ 4000 in RNA-Seq studies. The high quality high-throughout sequencing data were explored using high performance computing (HPC) in a petascale data center and subjected to de novo assembly and parallelized mpiBLASTX search with multiple species. As a result, a transcriptome database of 17,845 was constructed (~95% completeness). This enlarged database constructed fueled the RNA-Seq data analysis, which was validated by a nitrogen deprivation (ND) study that induces triacylglycerol (TAG) production.
  
  
  CONCLUSIONS
  The new paralleled assembly and annotation method under HPC presented here allows the solution of large-scale data processing problems in acceptable computation time. There is significant increase in the number of transcriptomic data achieved and observable heterogeneity in the performance to identify differentially expressed genes in the ND treatment paradigm. The results provide new insights as to how response to ND treatment in microalgae is regulated. ND analyses highlight the advantages of this database generated in this study that could also serve as a useful resource for future gene manipulation and transcriptome-wide analysis. We thus demonstrate the usefulness of exploring the transcriptome as an informative platform for functional studies and genetic manipulations in similar species.
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117545, Singapore.
authors.forename: Lina
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117545, Singapore.
authors.forename: Kenneth Wei Min
authors.initials: KW
authors.lastname: Tan
authors.affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117596, Singapore.|National Supercomputing Centre (NSCC), Singapore, 138632, Singapore.
authors.forename: Tin Wee
authors.initials: TW
authors.lastname: Tan
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117545, Singapore. yuan_kun_lee@nuhs.edu.sg.
authors.forename: Yuan Kun
authors.initials: YK
authors.lastname: Lee
mesh_terms: D000460:Chlorophyta / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D030541:Databases, Genetic; D015536:Down-Regulation; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D009584:Nitrogen / Q000378:metabolism; D017423:Sequence Analysis, RNA; D012984:Software; D059467:Transcriptome; D014280:Triglycerides / Q000096:biosynthesis; D015854:Up-Regulation
chemical_list: D014280:Triglycerides; D009584:Nitrogen
keywords: Dunaliella tertiolecta; HPC; Microalgae; RNA-Seq; Transcriptome