title: The clinical significance and biological function of large tumour suppressor 2 in hepatocellular carcinoma.
abstract: OBJECTIVE
  Present evidence has suggested that large tumour suppressor 2 (LATS2) is abnormally expressed in most human cancer. However, the clinical and prognostic value in hepatocellular carcinoma (HCC) is still unknown.
  
  
  METHODS
  Large tumour suppressor 2 mRNA and protein expression levels in HCC tissues and cell lines were detected by qRT-PCR, immunohistochemistry or Western blot. The correlation between LATS2 expression and clinicopathological factors was analysed through immunohistochemistry. The function of LATS2 on HCC cell growth and mobility was explored through MTT, colony formation, Transwell migration and invasion assays. The molecular mechanism of LATS2 was screened and confirmed by qRT-PCR and Western blot.
  
  
  CONCLUSIONS
  In this study, LATS2 mRNA and protein expressions were decreased in HCC tissues and cell lines compared with normal hepatic tissues and hepatic cell line. Low LATS2 expression was oppositely corrected with tumour stage, vascular invasion and metastasis. The univariate and multivariate analyses suggested that low LATS2 expression was an independent poor prognostic factor for HCC patients. The in vitro experiments showed that LATS2 regulated HCC cells migration and invasion, but had no effect on HCC cells proliferation. Meanwhile, LATS2 modulated metastasis-associated genes expression including E-cadherin, vimentin, snail, slug, MMP2 and MMP9. In conclusion, LATS2 is a prognostic biomarker and a tumour metastasis suppressor in HCC.
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Yuan
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Li
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Luo
authors.affiliation: Department of Hepatobilliary Surgery, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Jia-Zhou
authors.initials: JZ
authors.lastname: Ye
authors.affiliation: Department of Hepatobilliary Surgery, Affiliated Minzu Hospital of Guangxi Medical University, Naning, China.
authors.forename: Hai-Hong
authors.identifier: http://orcid.org/0000-0002-2764-6173
authors.initials: HH
authors.lastname: Ye
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: 
  KPM
  ; LATS2; biomarker; hepatocellular carcinoma