title: Expression of theaac(6')-Ib-crGene in Class 1 Integrons.
abstract: aac(6')-Ib-cris a plasmid-mediated quinolone resistance gene embedded within a gene cassette, most often within an integron. It confers resistance to quinolones and aminoglycosides. We investigated the role of a 101-bp fragment frequently present upstream of theaac(6')-Ib-crgene cassette and found that it contributes to the expression ofaac(6')-Ib-crand provides an alternative start codon, confirming the length of the AAC(6')-Ib-cr protein to 199 amino acids.
authors.affiliation: University of Limoges, UMR_S1092, Limoges, France sophie.raherison@unilim.fr.|INSERM, U1092, Limoges, France.|CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Raherison
authors.affiliation: University of Limoges, UMR_S1092, Limoges, France.|INSERM, U1092, Limoges, France.|CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Jove
authors.affiliation: University of Limoges, UMR_S1092, Limoges, France.|INSERM, U1092, Limoges, France.|CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Margaux
authors.initials: M
authors.lastname: Gaschet
authors.affiliation: University of Limoges SCRABL, Limoges, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Pinault
authors.affiliation: University of Limoges, UMR_S1092, Limoges, France.|INSERM, U1092, Limoges, France.|CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Tabesse
authors.affiliation: Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
authors.affiliation: University of Limoges, UMR_S1092, Limoges, France.|INSERM, U1092, Limoges, France.|CHU Limoges, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France.
authors.forename: Marie-Cécile
authors.initials: MC
authors.lastname: Ploy
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000235:genetics; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D015363:Quinolones / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase; D001616:beta-Galactosidase
keywords: AAC(6′)-Ib-cr protein; antibiotic resistance; gene cassette expression