title: Tissue-specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species.
abstract: The mitochondrial phosphate carrier gene (PiC) encodes a membrane protein that mediates the supply of inorganic phosphate from the cytosol into the mitochondrial matrix. This substrate-specific transport system plays an important role in efficient ATP synthesis. Mammals appear to have only one PiC with two alternative splicing variants whose functional differences remain unclear. The present study is the first to characterize the multiple genes that encode PiC in insects. Bombyx mori was found to have two PiC paralogues, one ubiquitous and one testis-specific, the latter seeming to be present only in Lepidoptera. Drosophila melanogaster was found to harbour two PiC paralogues, whereas Liriomyza chinensis, another dipteran, has three PiC paralogues. Two PiCs were found to be present in Plautia stali, and silencing either of these genes affected the normal development of P. stali nymphs, although their expression patterns differed amongst tissues. Schistocerca gregaria and Locusta migratoria have two PiC each, with different expression patterns. Tribolium castaneum was found to have only one PiC, which appears to play an essential role in larval development. Thus, although the inorganic phosphate transport system appears to be conserved across eukaryotes, PiC has become specialized in the different tissues of different insect species.
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Sugahara
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Jouraku
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakakura
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Minaba
authors.affiliation: Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Miyoshi
authors.affiliation: National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shiotsuki
mesh_terms: D000818:Animals; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007313:Insecta / Q000235:genetics / Q000378:metabolism*; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D019476:Insect Proteins; D024101:Mitochondrial Proteins; D028061:Phosphate Transport Proteins
keywords: RNA interference (RNAi); cloning; gene expression; insect; mitochondrial transport; phosphate carrier