title: MiR-144 affects fatty acid composition by regulating ELOVL6 expression in duck hepatocytes.
abstract: Lipid metabolism in duck is very important for both raisers and people's health. In our previous studies, we have detected that miR-144 is related to duck lipid metabolism and validated one of its target genes, elongation of very long chain fatty acids protein 6 (ELOVL6). In the present study, we isolated, cultured, and identified duck hepatocytes, and transfected with miR-144 mimics/inhibitor to mediate the miR-144 level. The qRT-PCR results showed that the ELOVL6 expression in duck hepatocytes was down/upregulated, respectively. The fat contents and each fatty-acid percent content of the hepatocytes and medium were also determined. When ELOVL6 expression suppressed (miR-144 mimics transfected), the palmitic acid (C16:0) content was significantly increased (P < 0.05); the oleic acid (C18:1, n-9), eicosenoic acid (C20:1, n-9), and eicosatrienoic acid (C20:3) contents were significantly reduced (P < 0.05). The myristic acid (C14:0) and palmitic acid (C16:0) contents were significantly reduced (P < 0.05), and the oleic acid (C18:1, n-9) content was significantly increased (P < 0.05) when ELOVL6 expression upregulated (miR-144 inhibitor transfected). It indicated that miR-144 could regulate some saturated fatty acids elongated to longer unsaturated fatty acids through controlling ELOVL6 expression. Whereas, miR-144/ELOVL6 appeared not associated with fat deposition in duck hepatocytes (P > 0.05). Our findings suggest that miR-144 might regulate the percentages of fatty acids in duck hepatocytes through affecting ELOVL6 expression.
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou, 310021, China.|Department of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, 325006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, 325006, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Tao
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Guoqin
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Animal Science, Zhejiang University, Hangzhou, 310021, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Niu
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Yingru
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D004372:Ducks / Q000235:genetics*; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D022781:Hepatocytes / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D061251:Primary Cell Culture / Q000379:methods
chemical_list: D005227:Fatty Acids; D035683:MicroRNAs; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases
keywords: ELOVL6; duck; hepatocyte; lipid metabolism; miR-144; microRNA