title: Bifidobacterium adolescentis Exerts Strain-Specific Effects on Constipation Induced by Loperamide in BALB/c Mice.
abstract: Constipation is one of the most common gastrointestinal complaints worldwide. This study was performed to determine whetherBifidobacterium adolescentisexerts inter-strain differences in alleviating constipation induced by loperamide in BALB/c mice and to analyze the main reasons for these differences. BALB/c mice underwent gavage withB. adolescentis(CCFM 626, 667, and 669) once per day for 17 days. The primary outcome measures included related constipation indicators, and the secondary outcome measures were the basic biological characteristics of the strains, the concentration changes of short-chain fatty acids in feces, and the changes in the fecal flora.B. adolescentisCCFM 669 and 667 relieved constipation symptoms by adhering to intestinal epithelial cells, growing quickly in vitro and increasing the concentrations of propionic and butyric acids. The effect ofB. adolescentison the gut microbiota in mice with constipation was investigated via 16S rRNA metagenomic analysis. The results revealed that the relative abundance ofLactobacillusincreased and the amount ofClostridiumdecreased in theB. adolescentisCCFM 669 and 667 treatment groups. In conclusion,B. adolescentisexhibits strain-specific effects in the alleviation of constipation, mostly due to the strains' growth rates, adhesive capacity and effects on the gut microbiome and microenvironment.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. wanglllynn09@163.com.|International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, China. wanglllynn09@163.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. 7130112038@vip.jiangnan.edu.cn.|International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, China. 7130112038@vip.jiangnan.edu.cn.
authors.forename: Lujun
authors.initials: L
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. 6140111107@vip.jiangnan.edu.cn.|International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, China. 6140111107@vip.jiangnan.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Kangyuan Dairy Co., Ltd., Yangzhou University, Yangzhou 225004, China. bxyin@yzu.edu.cn.
authors.forename: Boxing
authors.initials: B
authors.lastname: Yin
authors.affiliation: Kangyuan Dairy Co., Ltd., Yangzhou University, Yangzhou 225004, China. fdsheng@yzu.edu.cn.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. wanggang@jiangnan.edu.cn.|International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, China. wanggang@jiangnan.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. jxzhao@jiangnan.edu.cn.|International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, China. jxzhao@jiangnan.edu.cn.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. zhanghao@jiangnan.edu.cn.|International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, China. zhanghao@jiangnan.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. chenwei66@jiangnan.edu.cn.|International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi 214122, China. chenwei66@jiangnan.edu.cn.|Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China. chenwei66@jiangnan.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D000930:Antidiarrheals / Q000009:adverse effects*; D001422:Bacterial Adhesion; D000070005:Bifidobacterium adolescentis / Q000187:drug effects / Q000502:physiology*; D015415:Biomarkers; D003248:Constipation / Q000175:diagnosis / Q000209:etiology* / Q000628:therapy; D003672:Defecation; D004195:Disease Models, Animal; D005232:Fatty Acids, Volatile / Q000737:chemistry / Q000378:metabolism; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005750:Gastric Juice; D019073:HT29 Cells; D006801:Humans; D008139:Loperamide / Q000009:adverse effects*; D008551:Melena; D051379:Mice; D064307:Microbiota; D019936:Probiotics / Q000008:administration & dosage
chemical_list: D000930:Antidiarrheals; D015415:Biomarkers; D005232:Fatty Acids, Volatile; D008139:Loperamide
keywords: Bifidobacterium adolescentis; adhesive; constipation; intestinal community structure; strain-specific