title: Integrative hepatoprotective efficacy comparison of raw and vinegar-baked Radix Bupleuri using nuclear magnetic resonance-based metabolomics.
abstract: Radix Bupleuri (RB), with a Chinese name Chaihu, is one of the most popular Traditional Chinese herbal drug. It can be baked with vinegar to afford vinegar-baked Radix Bupleuri (VBRB), which is used in Traditional Chinese Medicine (TCM) for liver diseases treatment. In the present study, nuclear magnetic resonance-based metabolomic approach was used to compare the liver protective effect of RB and two types of VBRBs, which were prepared by two kinds of vinegar. The contents of 14 metabolites in the liver of carbon tetrachloride (CCl4) treated mice were significantly altered in comparison with control group, and VBRB prepared by Shanxi vinegar showed best effect as revealed by the amount and regulatory degree of the perturbed metabolites. The metabolism pathways analysis showed that the liver protective effect was related with the energy metabolism, lipid metabolism, ketone body metabolism, glutathione metabolism, amino acids metabolism and nucleotide synthesis. The results presented here showed that metabolomic approach made it possible to disclose the subtle biological difference between two types of VBRB, which highlight the potential of metabolomic approach to quantitatively compare the pharmacological effect of the herbal drugs.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People's Republic of China; College of Chemistry and Chemical Engineering of Shanxi University, No. 92, Wucheng Road 92, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xing
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Sun
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Jia
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People's Republic of China. Electronic address: qinxm@sxu.edu.cn.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Qin
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People's Republic of China. Electronic address: lizhenyu@sxu.edu.cn.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Li
mesh_terms: D019342:Acetic Acid / Q000737:chemistry*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D029972:Bupleurum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods; D055432:Metabolomics / Q000379:methods; D051379:Mice; D009711:Nucleotides / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D004365:Drugs, Chinese Herbal; D007657:Ketone Bodies; D009711:Nucleotides; D010936:Plant Extracts; D020011:Protective Agents; D005978:Glutathione; D019342:Acetic Acid
keywords: Drug efficacy index; Metabolomics; Nuclear magnetic resonance; Radix Bupleuri; Vinegar-baked Radix Bupleuri