title: Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance.
abstract: Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing OsMTI-1b showed more tolerance to Cd2+and accumulated more Cd2+ions when they were grown in the medium containing CdCl2. In addition, the heterologous expression of GST-OsMTI-1b conferred H2O2and ethanol tolerance to S. cerevisiae cells. The results indicate that heterologous expression of plant MT isoforms can enhance the tolerance of S. cerevisiae to multiple stresses.
authors.affiliation: Isfahan University of Technology, College of Agriculture, Department of Biotechnology, Isfahan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ansarypour
authors.affiliation: Isfahan University of Technology, College of Agriculture, Department of Biotechnology, Isfahan, Iran. Electronic address: a.shahpiri@cc.iut.ac.ir.
authors.forename: Azar
authors.initials: A
authors.lastname: Shahpiri
mesh_terms: D002104:Cadmium / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics*; D018384:Oxidative Stress; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020033:Protein Isoforms; D002104:Cadmium; D000431:Ethanol; D008668:Metallothionein; D006861:Hydrogen Peroxide
keywords: Cadmium; Ethanol; Hydrogen peroxide; Rice metallothionein; S. cerevisiae