title: A Proof of Concept to Bridge the Gap between Mass Spectrometry Imaging, Protein Identification and Relative Quantitation: MSI~LC-MS/MS-LF.
abstract: Mass spectrometry imaging (MSI) is a powerful tool to visualize the spatial distribution of molecules on a tissue section. The main limitation of MALDI-MSI of proteins is the lack of direct identification. Therefore, this study focuses on a MSI~LC-MS/MS-LF workflow to link the results from MALDI-MSI with potential peak identification and label-free quantitation, using only one tissue section. At first, we studied the impact of matrix deposition and laser ablation on protein extraction from the tissue section. Then, we did a back-correlation of them/zof the proteins detected by MALDI-MSI to those identified by label-free quantitation. This allowed us to compare the label-free quantitation of proteins obtained in LC-MS/MS with the peak intensities observed in MALDI-MSI. We managed to link identification to nine peaks observed by MALDI-MSI. The results showed that the MSI~LC-MS/MS-LF workflow (i) allowed us to study a representative muscle proteome compared to a classical bottom-up workflow; and (ii) was sparsely impacted by matrix deposition and laser ablation. This workflow, performed as a proof-of-concept, suggests that a single tissue section can be used to perform MALDI-MSI and protein extraction, identification, and relative quantitation.
authors.affiliation: Institut National de la Recherche Agronomique (INRA), Plateforme d'Exploration du Métabolisme (PFEM), F-63122 Saint Genès Champanelle, France. laetitiathrn@gmail.com.
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Théron
authors.affiliation: Institut National de la Recherche Agronomique (INRA), Plateforme d'Exploration du Métabolisme (PFEM), F-63122 Saint Genès Champanelle, France. delphine.centeno@inra.fr.
authors.forename: Delphine
authors.initials: D
authors.lastname: Centeno
authors.affiliation: INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, F-63122 Saint Genès Champanelle, France. cecile.coudy-gandilhon@inra.fr.|Clermont Université, Université d'Auvergne, F-63000 Clermont-Ferrand, France. cecile.coudy-gandilhon@inra.fr.
authors.forename: Cécile
authors.initials: C
authors.lastname: Coudy-Gandilhon
authors.affiliation: Institut National de la Recherche Agronomique (INRA), Plateforme d'Exploration du Métabolisme (PFEM), F-63122 Saint Genès Champanelle, France. estelle.pujos-guillot@inra.fr.
authors.forename: Estelle
authors.initials: E
authors.lastname: Pujos-Guillot
authors.affiliation: INRA Clermont-Ferrand Theix, UR370 Qualité des Produits Animaux, F-63122 Saint-Genès-Champanelle, France. thierry.astruc@inra.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Astruc
authors.affiliation: INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, F-63122 Saint Genès Champanelle, France. didier.remond@inra.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Rémond
authors.affiliation: PRES Lyon, SNA-EPIS Research Unit, Exercise and Clinical Physiology Laboratory, University Hospital and Jean Monnet University, F-42023 Saint-Etienne, France. jc.barthelemy@univ-st-etienne.fr.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Barthelemy
authors.affiliation: PRES Lyon, SNA-EPIS Research Unit, Exercise and Clinical Physiology Laboratory, University Hospital and Jean Monnet University, F-42023 Saint-Etienne, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Roche
authors.affiliation: Unité de Myologie, LPE-EA4338, UJM/CHU, F-42055 Saint-Etienne, France. leonard.feasson@univ-st-etienne.fr.
authors.forename: Léonard
authors.initials: L
authors.lastname: Feasson
authors.affiliation: Institut National de la Recherche Agronomique (INRA), Plateforme d'Exploration du Métabolisme (PFEM), F-63122 Saint Genès Champanelle, France. michel.hebraud@inra.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Hébraud
authors.affiliation: INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, F-63122 Saint Genès Champanelle, France. daniel.bechet@inra.fr.|Clermont Université, Université d'Auvergne, F-63000 Clermont-Ferrand, France. daniel.bechet@inra.fr.
authors.forename: Daniel
authors.initials: D
authors.lastname: Béchet
authors.affiliation: INRA, UMR 1019, Unité de Nutrition Humaine, CRNH Auvergne, F-63122 Saint Genès Champanelle, France. christophe.chambon@clermont.inra.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Chambon
keywords: MALDI mass spectrometry imaging; label-free quantitation; protein identification; skeletal muscle