title: Systematic identification of flavonols, flavonol glycosides, triterpene and siraitic acid glycosides from Siraitia grosvenorii using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combined with a screening strategy.
abstract: The fruits of Siraitia grosvenorii are considered to be health-promoting because of the diversity of their bioactive ingredients. In the present study, a screening method, using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening strategy, has been established. The technology was used to systematically screening the targeted metabolites, primarily from the complex matrix of S. grosvenorii. The compounds were then identified by their exact masses and characteristic fragment ions, in comparison with the fragmentation behaviors of 19 references. Finally, 122 compounds, including 53 flavonols and flavonol glycosides, 59 triterpene glycosides and 10 siraitic acid glycosides, were screened and identified in 10-, 50- and 80-day fruits, roots, stems and leaves of S. grosvenorii. 98 of them were reported for the first time. Additionally, the distribution of all identified components in different parts of the plant was determined and metabolic networks for flavonol and triterpene glycosides were proposed.
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: 719764893@qq.com.
authors.forename: Zhi-Xing
authors.initials: ZX
authors.lastname: Qing
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. Electronic address: 53522722@163.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: tangqi423@sina.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China. Electronic address: mochming@163.com.
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Mo
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: phuang1985@126.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: 18027474@qq.com.
authors.forename: Pi
authors.initials: P
authors.lastname: Cheng
authors.affiliation: College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: 137292113@qq.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: 21811025@qq.com.
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Yang
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: 87135168@qq.com.
authors.forename: Xiu-Bin
authors.initials: XB
authors.lastname: Liu
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: zhengyj4@126.com.
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Zheng
authors.affiliation: National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: zengjianguo@hunau.edu.cn.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zeng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044948:Flavonols / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000737:chemistry*
chemical_list: D044948:Flavonols; D006027:Glycosides; D014315:Triterpenes
keywords: Flavonol glycosides; HPLC-Q-TOF-MS; Siraitia grosvenorii; Siraitic acid glycosides; Triterpene glycosides