title: Development of novel murine mammary imaging windows to examine wound healing effects on leukocyte trafficking in mammary tumors with intravital imaging.
abstract: We developed mammary imaging windows (MIWs) to evaluate leukocyte infiltration and cancer cell dissemination in mouse mammary tumors imaged by confocal microscopy. Previous techniques relied on surgical resection of a skin flap to image the tumor microenvironment restricting imaging time to a few hours. Utilization of mammary imaging windows offers extension ofintravitalimaging of the tumor microenvironment. We have characterized strengths and identified some previously undescribed potential weaknesses of MIW techniques. Through iterative enhancements of a transdermal portal we defined conditions for improved quality and extended confocal imaging time for imaging key cell-cell interactions in the tumor microenvironment.
authors.affiliation: Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN, USA; Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Tammy
authors.initials: T
authors.lastname: Sobolik
authors.affiliation: Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN, USA; Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Su
authors.affiliation: Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN, USA; Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Will
authors.initials: W
authors.lastname: Ashby
authors.affiliation: Department of Physics and Astronomy and the Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University , Nashville, TN, USA.
authors.forename: David K
authors.initials: DK
authors.lastname: Schaffer
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine , Nashville, TN, USA.
authors.forename: Sam
authors.initials: S
authors.lastname: Wells
authors.affiliation: Department of Physics and Astronomy and the Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, TN, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Wikswo
authors.affiliation: Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN, USA; Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Andries
authors.initials: A
authors.lastname: Zijlstra
authors.affiliation: Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN, USA; Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Richmond
keywords: breast cancer; cell migration; intravital imaging; mammary imaging windows