title: [Effect of Gas6 in silica-induced inflammation on differentiated human acute monocytic leukemia (THP-1) macrophages].
abstract: Objective:To investigate the modulation role of Gas6 in silica-induced inflammatory effect on human macrophages.Methods:Differentiated THP-1 macrophages were exposed to different concentrations of silica for 6 h and 24 h. Additionally, silica-activated macrophages were treated with different concentrations of recombine human Gas6 and Gas6 antibody respectively. Cell viabilities were determined by CCK-8 kit. Expression levels of Gas6 and inflammatory cytokines (TNF-α, IL-1β and IL-6) were measured by ELISA assay kits.Results:Silica particles induced clear dose-dependent decreases of cell viability and Gas6 expression at both 6 h and 24 h. The cell viability of 24 h is lower than 6 h at the same concentration of silica (P<0.05). Furthermore, silica activated macrophages treated with Gas6 antibody induced significant decreases of Gas6 both at 6 h and 24 h (P<0.05). After pretreated with various concentrations of Gas6 antibody, silica induced higher expressions of inflammatory cytokines (TNF-α, IL-1β, IL-6) in dose-dependent manners at two time points. Addition of exoge-nous Gas6 significantly suppressed silica-induced inflammatory cytokines concentrations mentioned above in the cell culture supernatants in clear dose-dependent manners.Conclusion:Exogenous Gas6 could inhibit the secre-tion of inflammatory cytokines in macrophages, while the block of Gas6 might enhance this inflammation.
authors.affiliation: Department of Occupational an Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.forename: X Q
authors.initials: XQ
authors.lastname: Cui
authors.forename: Y
authors.initials: Y
authors.lastname: Rong
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.forename: L L
authors.initials: LL
authors.lastname: Xiao
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhang
authors.forename: W H
authors.initials: WH
authors.lastname: Chen
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007948:Leukemia, Monocytic, Acute; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000276:immunology; D012822:Silicon Dioxide / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D000099255:Growth Arrest-Specific Protein 6
chemical_list: D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D012822:Silicon Dioxide; D014409:Tumor Necrosis Factor-alpha; D000099255:Growth Arrest-Specific Protein 6
keywords: Gas6; Macrophage; Quartz