title: The susceptibility of Staphylococcus aureus CIP 65.8 and Pseudomonas aeruginosa ATCC 9721 cells to the bactericidal action of nanostructured Calopteryx haemorrhoidalis damselfly wing surfaces.
abstract: Nanostructured insect wing surfaces have been reported to possess the ability to resist bacterial colonization through the mechanical rupture of bacterial cells coming into contact with the surface. In this work, the susceptibility of physiologically young, mature and old Staphylococcus aureus CIP 65.8 and Pseudomonas aeruginosa ATCC 9721 bacterial cells, to the action of the bactericidal nano-pattern of damselfly Calopteryx haemorrhoidalis wing surfaces, was investigated. The results were obtained using several surface characterization techniques including optical profilometry, scanning electron microscopy, synchrotron-sourced Fourier transform infrared microspectroscopy, water contact angle measurements and antibacterial assays. The data indicated that the attachment propensity of physiologically young S. aureus CIP 65.8Tand mature P. aeruginosa ATCC 9721 bacterial cells was greater than that of the cells at other stages of growth. Both the S. aureus CIP 65.8Tand P. aeruginosa ATCC 9721 cells, grown at the early (1 h) and late stationary phase (24 h), were found to be most susceptible to the action of the wings, with up to 89.7 and 61.3% as well as 97.9 and 97.1% dead cells resulting from contact with the wing surface, respectively.
authors.affiliation: School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria, 3122, Australia.
authors.forename: Vi Khanh
authors.initials: VK
authors.lastname: Truong
authors.affiliation: School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria, 3122, Australia.
authors.forename: Nipuni Mahanamanam
authors.initials: NM
authors.lastname: Geeganagamage
authors.affiliation: Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, 26 Av. dels Paisos Catalans, 43007, Tarragona, Spain.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Baulin
authors.affiliation: Infrared Microspectroscopy Beamline, Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria, 3168, Australia.
authors.forename: Jitraporn
authors.initials: J
authors.lastname: Vongsvivut
authors.affiliation: Infrared Microspectroscopy Beamline, Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria, 3168, Australia.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Tobin
authors.affiliation: Museu de les Terres de l'Ebre, Gran Capità, 34, 43870, Amposta, Spain.
authors.forename: Pere
authors.initials: P
authors.lastname: Luque
authors.affiliation: School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, Victoria, 3001, Australia.
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Crawford
authors.affiliation: School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria, 3122, Australia. eivanova@swin.edu.au.
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
mesh_terms: D000818:Animals; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D063191:Odonata / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000502:physiology*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000502:physiology*; D013499:Surface Properties; D014921:Wings, Animal / Q000382:microbiology* / Q000648:ultrastructure
keywords: Bactericidal; Black damselfly; Calopteryx haemorrhoidalis; Nanopillar arrays; Physiological growth