title: Characterization of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pigs and Farm Workers.
abstract: Food-producing animals can serve as reservoirs for extended-spectrum β-lactamase (ESBL)-producing Escherichia coli . The present study aimed to characterize and compare ESBL-carrying E. coli isolates from both pigs and farm workers. Rectal swabs were obtained from 60 pigs on four pig-fattening farms (15 samples per farm), and rectal swabs were taken from 40 farm workers on these farms (10 samples per farm). ESBL-carrying E. coli isolates from the workers and pigs were characterized by ESBL genotype, antibiotic susceptibility, enterobacterial repetitive intergenic consensus type, and multilocus sequence type. ESBL-producing E. coli was detected in 34 (56.7%) of 60 pigs, and 20.0% (8 of 40) of the farm workers were positive for ESBL-producing E. coli . More importantly, ESBL-producing E. coli isolates with the same β-lactamase genes, antibiotic resistance profiles, enterobacterial repetitive intergenic consensus types, and multilocus sequence types were detected in both pigs and workers on the same pig farm. These findings were suggestive for transfer of ESBL-producing E. coli between animals and humans.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Hongna
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Digestive Disease Institute, Central Hospital of Tai'an City, Tai'an 271000, People's Republic of China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Zhai
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Linghong
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Qimeng
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Ye
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Weishan
authors.initials: W
authors.lastname: Chang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhai
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D000072480:Farms; D005243:Feces / Q000382:microbiology; D006801:Humans; D013552:Swine; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases
keywords: Escherichia coli; Extended-spectrum β-lactamase; Farm workers; Pigs; Transfer