title: Boswellia serrata resin extract alleviates azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon tumorigenesis.
abstract: Boswellia serrata (BS) resin is a popular dietary supplement for joint nourishment. In this study, we investigated the chemopreventive effects of dietary BS extract and its impact of gut microbiota on azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colitis-associated colon cancer in mice.
  
  
  
  Male ICR mice were injected with AOM and 2% DSS via drinking water. The mice were fed with 0.25 or 0.5% BS extract, and colonic tissue were collected at 15 weeks. The main effective components of BS supercritical CO2extraction were analyzed by LC-MS/MS are boswellic acids. We found that treatment with BS extract significantly reduce the colonic tumor formation. Western blot and histological analysis revealed that dietary BS extract could markedly reduce the inflammation associated protein levels expression. Furthermore, BS extract reduced cell proliferation via inhibiting phosphorylation level of protein kinase B (Akt), glycogen synthase kinase 3β (GSK3β), and downregulation of cyclin D1. In addition, BS extract also altered the composition of gut microbiota by enhancing the proportion of Clostridiales and reducing the percentage of Bacteroidales.
  
  
  
  In summary, BS extract decreased the protein levels of inflammative enzymes such as inducible nitric oxide synthase and cyclooxygenase-2 in colonic mucosa. It also mediated Akt/GSK3β/cyclin D1 signaling pathway and altered the composition of gut microbiota to alleviate tumor growth. Taken together, this study suggests that BS extract has great potential to suppress colon tumorigenesis.
authors.affiliation: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Ya-Chun
authors.initials: YC
authors.lastname: Chou
authors.affiliation: Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
authors.forename: Joon Hyuk
authors.initials: JH
authors.lastname: Suh
authors.affiliation: Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: India Glycols Ltd., Noida, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Pahwa
authors.affiliation: American Medical Holdings Incorporated, New York, NY, USA.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Badmaev
authors.affiliation: Department of Food Science, Rutgers University, New Brunswick, NJ, USA.
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.affiliation: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Min-Hsiung
authors.initials: MH
authors.lastname: Pan
mesh_terms: D000818:Animals; D001397:Azoxymethane; D029743:Boswellia; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D019938:Cyclin D1 / Q000037:antagonists & inhibitors; D016264:Dextran Sulfate; D000071679:Glycogen Synthase Kinase 3 beta / Q000037:antagonists & inhibitors; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors; D012116:Resins, Plant / Q000494:pharmacology*
chemical_list: C530105:Ccnd1 protein, mouse; D010936:Plant Extracts; D012116:Resins, Plant; D019938:Cyclin D1; D016264:Dextran Sulfate; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D001397:Azoxymethane
keywords: Azoxymethane; Boswellia Serrata; Colorectal cancer; Dextran sulfate sodium; Inflammation