title: DNA-SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells.
abstract: A novel SMART module, dubbed "DNA-SMART" (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single-stranded DNA capture strands, microthermoformed into 3D structures, and postmodified with complementary DNA-protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, D-76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Ann-Kathrin
authors.initials: AK
authors.lastname: Schneider
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, D-76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Pavel M
authors.initials: PM
authors.lastname: Nikolov
authors.affiliation: MERLN Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40 6229, ER Maastricht, PO Box 616, 6200, MD, Maastricht, The Netherlands.
authors.forename: Stefan
authors.initials: S
authors.lastname: Giselbrecht
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, D-76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D056656:Lab-On-A-Chip Devices; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011108:Polymers; D011506:Proteins; D004247:DNA
keywords: DNA; cells; fluidics; microstructures; proteins