title: Hollow fiber membrane bioreactor affects microbial community and morphology of the DAMO and Anammox co-culture system.
abstract: Denitrifying anaerobic methane oxidation (DAMO) and Anammox co-culture system was investigated in hollow fiber membrane bioreactor (HfMBR) for the change of microbial community morphology and proportion. NO3--N and NH4+-N removal rates reached 85.33 and 37.95mg/L/d on 193d. The inoculum microorganisms were flocs and the proportion of DAMO archaea, DAMO bacteria and Anammox bacteria was 11.0, 24.2 and 0.4%, respectively, but it changed to 74.3, 11.8, 5.6% in HfMBR, respectively. Interestingly, microorganisms formed biofilms on fibers surface and the biofilms included two layers: inner layer was thin and dominated by DAMO bacteria and Anammox bacteria; while the outer layer was thick made up of granules with 100-200μm diameter and dominated by DAMO archaea. The spatial distribution of microorganisms in HfMBR was different from simulation results in the literature. Likely, HfMBR changed the interaction between DAMO and Anammox microorganisms, and the reactor configuration was beneficial for DAMO archaea growth.
authors.affiliation: CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China; School of Environment, Northeast Normal University, Changchun 130117, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Fu
authors.affiliation: CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China; Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou 215213, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ding
authors.affiliation: CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
authors.forename: Yong-Ze
authors.initials: YZ
authors.lastname: Lu
authors.affiliation: CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
authors.forename: Zhao-Wei
authors.initials: ZW
authors.lastname: Ding
authors.affiliation: CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China; Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou 215213, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Bai
authors.affiliation: CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China; Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou 215213, China. Electronic address: rzeng@ustc.edu.cn.
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Zeng
mesh_terms: D064751:Ammonium Compounds / Q000737:chemistry*; D000693:Anaerobiosis; D001105:Archaea / Q000254:growth & development* / Q000378:metabolism; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D018920:Coculture Techniques; D058440:Denitrification; D008567:Membranes, Artificial; D008697:Methane / Q000378:metabolism*; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods; D010084:Oxidation-Reduction
chemical_list: D064751:Ammonium Compounds; D008567:Membranes, Artificial; D008697:Methane
keywords: Anammox; Denitrifying anaerobic methane oxidation; Granule; Hollow fiber membrane bioreactor; Microbial community