title: Lack of germline mutation at codon 211 of the prion protein gene (PRNP) in Korean native cattle - Short communication.
abstract: Bovine prion diseases are composed of two types of bovine spongiform encephalopathy (BSE), classical BSE and atypical BSE. Recent studies have identified one case of atypical BSE with an E211K mutation. E211K is homologous to the human E200K mutation, which is related to familial Creutzfeldt-Jakob disease (CJD), one of the familial forms of human prion diseases. To date, familial forms of prion diseases have not been reported in non-human animals. Because the familial forms of human prion diseases account for more than 10% of all human prion disease cases, the detection of the E211K mutation in healthy cattle is very important for verifying the role of this mutation as a familial form of BSE. To detect putative mutations related to familial BSE, specifically E211K in Korean native cattle (Hanwoo) and Korean dairy cattle (Holstein), we performed direct sequencing targeting codon 211 and the adjacent regions of the bovine prion protein (PRNP) gene in 384 Hanwoo and 152 Holstein cattle. We did not find the E211K mutation in any of the Korean cattle. Although we did not find the E211K mutation in Korean native cattle, E211K is a postulated mutation; therefore, further screening in other countries and larger samples is highly desirable.
authors.affiliation: Korea Zoonosis Research Institute, Chonbuk National University , Iksan, Jeonbuk 570-390 , Republic of Korea.|Department of Bioactive Material Sciences, Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Yong-Chan
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Korea Zoonosis Research Institute, Chonbuk National University , Iksan, Jeonbuk 570-390 , Republic of Korea.|Department of Bioactive Material Sciences, Chonbuk National University , Jeonju , Republic of Korea.
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Jeong
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D018095:Germ-Line Mutation; D000072002:Prion Proteins / Q000235:genetics*; D056910:Republic of Korea
chemical_list: D000072002:Prion Proteins
keywords: Bovine; codon 211; mutation; prion protein gene