title: The scaffolding protein NHERF1 regulates the stability and activity of the tyrosine kinase HER2.
abstract: We examined whether the scaffolding protein sodium-hydrogen exchanger regulatory factor 1 (NHERF1) interacts with the calcium pump PMCA2 and the tyrosine kinase receptor ErbB2/HER2 in normal mammary epithelial cells and breast cancer cells. NHERF1 interacts with the PDZ-binding motif in PMCA2 in both normal and malignant breast cells. NHERF1 expression is increased in HER2-positive breast cancers and correlates with HER2-positive status in human ductal carcinomain situ(DCIS) lesions and invasive breast cancers as well as with increased mortality in patients. NHERF1 is part of a multiprotein complex that includes PMCA2, HSP90, and HER2 within specific actin-rich and lipid raft-rich membrane signaling domains. Knocking down NHERF1 reduces PMCA2 and HER2 expression, inhibits HER2 signaling, dissociates HER2 from HSP90, and causes the internalization, ubiquitination, and degradation of HER2. These results demonstrate that NHERF1 acts with PMCA2 to regulate HER2 signaling and membrane retention in breast cancers.
authors.affiliation: From the Section of Endocrinology and Metabolism, Department of Internal Medicine.
authors.forename: Jaekwang
authors.initials: J
authors.lastname: Jeong
authors.affiliation: From the Section of Endocrinology and Metabolism, Department of Internal Medicine.
authors.forename: Joshua N
authors.initials: JN
authors.lastname: VanHouten
authors.affiliation: From the Section of Endocrinology and Metabolism, Department of Internal Medicine.
authors.forename: Wonnam
authors.initials: W
authors.lastname: Kim
authors.affiliation: From the Section of Endocrinology and Metabolism, Department of Internal Medicine.
authors.forename: Pamela
authors.initials: P
authors.lastname: Dann
authors.affiliation: Department of Pediatrics, and.
authors.forename: Catherine
authors.initials: C
authors.lastname: Sullivan
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520.
authors.forename: Jungmin
authors.initials: J
authors.lastname: Choi
authors.affiliation: the Laboratory for GPCR Biology, Department of Pharmacology and Chemical Biology, and.
authors.forename: W Bruce
authors.initials: WB
authors.lastname: Sneddon
authors.affiliation: the Laboratory for GPCR Biology, Department of Pharmacology and Chemical Biology, and.|Department of Structural Biology,University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Friedman
authors.affiliation: From the Section of Endocrinology and Metabolism, Department of Internal Medicine, john.wysolmerski@yale.edu.
authors.forename: John J
authors.initials: JJ
authors.lastname: Wysolmerski
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D010750:Phosphoproteins / Q000378:metabolism*; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*
chemical_list: D002118:Calcium; D018841:HSP90 Heat-Shock Proteins; D010750:Phosphoproteins; D053499:Plasma Membrane Calcium-Transporting ATPases; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor; C508053:ERBB2 protein, human; C506958:ATP2B2 protein, human
keywords: PDZ domain; breast cancer; calcium transport; heat shock protein 90 (Hsp90); receptor internalization