title: Evaluation of chemerin and its receptors, ChemR23 and CCRL2, in gingival tissues with healthy and periodontitis.
abstract: Chemerin is a chemoattractant protein that directs inflammatory cells that express its receptor chemokine receptor-like 1 (ChemR23) towards sites of inflammation. C-C chemokine receptor-like 2 (CCRL2), is the other receptor of chemerin, improves the interaction between chemerin and ChemR23. The aim of this study was to evaluate the expression of chemerin and its receptors in gingival tissues with healthy and periodontitis. Tissue biopsy samples were obtained from 20 patients with chronic periodontitis and from the gingiva of 20 healthy individuals undergoing a crown lengthening process. Quantitative real-time PCR (qPCR) was used to examine the mRNA expression of chemerin, ChemR23 and CCRL2. Additionally, protein expression was measured by immunohistochemistry. Both qPCR and immunohistochemistry results revealed that the expression of chemerin and ChemR23 was significantly higher in tissues with periodontitis than in healthy tissues (P = 0.001 and, P = 0.015, respectively). There were no significant differences between healthy tissues and those with periodontitis in terms of mRNA expression of CCRL2, whereas a more intense staining was observed in tissues with periodontitis. The mRNA expression levels of chemerin showed a positive correlation with plaque index, gingival index, probing pocket depth and clinical attachment level (r = 0.448, r = 0.460, r = 0.439 and, r = 0.459, respectively, P < 0.01). To the best of our knowledge, this study is the first to examine the expression of chemerin, ChemR23 and CCRL2 in gingival tissues. Our study suggests that chemerin may play a role in the pathogenesis of periodontitis by causing chemoattraction of immune cells that direct ChemR23 receptors to the site of inflammation.
authors.affiliation: Department of Periodontology, Center of Oral Health, Gülhane Medical Academy, Ankara, 06018, Turkey. drdterkan@mynet.com.
authors.forename: Erkan
authors.initials: E
authors.lastname: Özcan
authors.affiliation: Department of Periodontology, Center of Oral Health, Gülhane Medical Academy, Ankara, 06018, Turkey.
authors.forename: N Işıl
authors.initials: NI
authors.lastname: Saygun
authors.affiliation: Department of Immunology, Gülhane Medical Academy, Ankara, Turkey.
authors.forename: Rahşan
authors.initials: R
authors.lastname: Ilıkçı
authors.affiliation: Department of Pathology, Gülhane Medical Academy, Ankara, Turkey.
authors.forename: Yıldırım
authors.initials: Y
authors.lastname: Karslıoğlu
authors.affiliation: Department of Immunology, Gülhane Medical Academy, Ankara, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Muşabak
authors.affiliation: Department of Immunology, Gülhane Medical Academy, Ankara, Turkey.
authors.forename: Sait
authors.initials: S
authors.lastname: Yeşillik
mesh_terms: D000328:Adult; D018925:Chemokines / Q000378:metabolism*; D055113:Chronic Periodontitis / Q000378:metabolism*; D003774:Dental Plaque Index; D005260:Female; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D010512:Periodontal Index; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D054388:Receptors, CCR / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*
chemical_list: C487531:CCRL2 protein, human; D018925:Chemokines; D036341:Intercellular Signaling Peptides and Proteins; C478630:RARRES2 protein, human; D012333:RNA, Messenger; D054388:Receptors, CCR; D019707:Receptors, Chemokine
keywords: CCRL2; ChemR23; Chemerin; Inflammation; Periodontitis