title: Protective effects of fentanyl preconditioning on cardiomyocyte apoptosis induced by ischemia-reperfusion in rats.
abstract: We aimed to study the effect of fentanyl (Fen) preconditioning on cardiomyocyte apoptosis induced by ischemia-reperfusion (I/R) in rats. A total of 120 Sprague Dawley male rats (age: 3 months) were randomly divided into: sham operation group (S group), I/R group, normal saline I/R group (NS group), and fentanyl low, middle, and high dose groups (Fen1: 2 μg/kg; Fen2: 4 μg/kg; Fen3: 6 μg/kg). Heart rate (HR), mean arterial pressure (MAP), left ventricular developed pressure (LVDP), ±dp/dtmax, malondialdehyde (MDA), superoxide dismutase (SOD) activity, creatine phosphokinase-MB (CK-MB), and cardiac troponin-I (cTnI) were measured. Myocardial ischemic (MI) area, total apoptotic myocardial cells, and protein and mRNA expressions of B-cell lymphoma 2 (Bcl-2) and Bax were detected. HR and MAP were higher, while LVDP and ±dp/dtmax were close to the base value in the Fen groups compared to those in the I/R group. Decreased MDA concentration and CK-MB value and increased SOD activity were found in the Fen groups compared to the I/R group, while cTnI concentration was significantly lower in the Fen1 and Fen2 groups (all P<0.05). Myocardial damage was less in the Fen groups compared to the I/R group and the MI areas and apoptotic indexes were significantly lower in the Fen1 and Fen2 groups (all P<0.05). Furthermore, significantly increased protein and mRNA expressions of Bcl-2, and decreased protein and mRNA expressions of Bax were found in the Fen groups compared to the I/R group (all P<0.05). Fentanyl preconditioning may suppress cardiomyocyte apoptosis induced by I/R in rats by regulating Bcl-2 and Bax.
authors.affiliation: Department of Anesthesiology, Linyi People's Hospital, Linyi, Shandong, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Anesthesiology, Linyi Cancer Hospital, Linyi, Shandong, China.
authors.forename: Q-G
authors.initials: QG
authors.lastname: Li
authors.affiliation: Operation Room, Linyi People's Hospital, Linyi, Shandong, China.
authors.forename: G-R
authors.initials: GR
authors.lastname: Fan
authors.affiliation: Department of Anesthesiology, Linyi People's Hospital, Linyi, Shandong, China.
authors.forename: Q-H
authors.initials: QH
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Linyi People's Hospital, Linyi, Shandong, China.
authors.forename: F-L
authors.initials: FL
authors.lastname: Mi
authors.affiliation: Department of Anesthesiology, Linyi People's Hospital, Linyi, Shandong, China.
authors.forename: B
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D005283:Fentanyl / Q000627:therapeutic use*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020011:Protective Agents; D005283:Fentanyl