title: Determination of glucose exchange rates and permeability of erythrocyte membrane in preeclampsia and subsequent oxidative stress-related protein damage using dynamic-19F-NMR.
abstract: The cause of the pregnancy condition preeclampsia (PE) is thought to be endothelial dysfunction caused by oxidative stress. As abnormal glucose tolerance has also been associated with PE, we use a fluorinated-mimic of this metabolite to establish whether any oxidative damage to lipids and proteins in the erythrocyte membrane has increased cell membrane permeability. Data were acquired using19F Dynamic-NMR (DNMR) to measure exchange of 3-fluoro-3-deoxyglucose (3-FDG) across the membrane of erythrocytes from 10 pregnant women (5 healthy control women, and 5 from women suffering from PE). Magnetisation transfer was measured using the 1D selective inversion and 2D EXSY pulse sequences, over a range of time delays. Integrated intensities from these experiments were used in matrix diagonalisation to estimate the values of the rate constants of exchange and membrane permeability. No significant differences were observed for the rate of exchange of 3-FDG and membrane permeability between healthy pregnant women and those suffering from PE, leading us to conclude that no oxidative damage had occurred at this carrier-protein site in the membrane.
authors.affiliation: Department of Chemistry, University of York, Heslington, York, UK. elizabeth.dickinson@york.ac.uk.
authors.forename: Elizabeth
authors.identifier: 0000-0001-8961-3230
authors.initials: E
authors.lastname: Dickinson
authors.affiliation: Selby College, Abbot's Road, Selby, North Yorkshire, YO8 8AT, UK.
authors.forename: John R P
authors.initials: JR
authors.lastname: Arnold
authors.affiliation: School of Chemistry, University of Leeds, Leeds, UK.
authors.forename: Julie
authors.initials: J
authors.lastname: Fisher
mesh_terms: D000328:Adult; D015415:Biomarkers; D002463:Cell Membrane Permeability; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D005461:Fluorine; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018384:Oxidative Stress; D011247:Pregnancy; D020543:Proteome; D040901:Proteomics / Q000379:methods; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism
chemical_list: D015415:Biomarkers; D020543:Proteome; D017382:Reactive Oxygen Species; D005461:Fluorine; D005947:Glucose
keywords: Dynamic; Exchange; Membrane; NMR; Oxidative stress; Preeclampsia