title: Evaluation of a thermostable Newcastle disease virus strain TS09-C as an in-ovo vaccine for chickens.
abstract: In-ovo vaccination is an attractive immunization approach for poultry industry. However, most of the Newcastle disease virus (NDV) vaccine strains used after hatch are unsafe, as in-ovo vaccines, due to their high pathogenicity for chicken embryos. In this study, we evaluated the safety and immunogenicity of a thermostable NDV strain TS09-C, derived from V4 strain, as in-ovo vaccine. Chickens in-ovo vaccinated with the parental V4 strain displayed greatly reduced hatchability and severe histopathological lesions in both trachea and intestine tissues, while the hatchability was not affected by in-ovo vaccination withTS09-C strain. The safe dose that infected all chicken embryos without obviously histopathological lesions was 103.0 EID50 per bird. In-ovo vaccination of chickens with TS09-C virus conferred complete protection against virulent NDV challenge. Results suggest that the thermostable NDV strain TS09-C is a safe and immunogenic in-ovo vaccine candidate that can be delivered quickly and uniformly, and induce earlier immune response.
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.|Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Wuhan, China.|Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China.
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Wen
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Lintao
authors.initials: L
authors.lastname: Li
authors.affiliation: US National Poultry Research Center, Agricultural Research Services, United States Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia, United States of America.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.|Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.|Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.|Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.|Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Wanpo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.|Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China.
authors.forename: Huabin
authors.initials: H
authors.lastname: Shao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens / Q000276:immunology*; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines