title: Irradiation pretreatment facilitates the achievement of high total sugars concentration from lignocellulose biomass.
abstract: This study evaluated the two hydrolysis strategies, involving one thermal and one dilute acid/enzymatic hydrolysis, to produce high xylose and glucose concentrations from lignocellulose assisted with irradiation pretreatment. Prior to hydrolysis, lignocellulose was pretreated by γ-irradiation at 800KGy. The merits of irradiation pretreatment on lignocellulose were contributed to size-reduced particle distributions and low shear rate of material, which allowed high biomass loadings up to 30-40%(w/v, equals to 23-29wt.%) for the consequent hydrolysis process. Results showed that hemicellulose fraction could achieve ∼84g/L of total sugars containing ∼55g/L xylose and ∼21g/L glucose through this two steps hydrolysis. Cellulose fraction would release ∼251g/L of total sugars consisting of ∼235g/L glucose and ∼16g/L xylose in the ultimate enzymatic hydrolysate. To the best of our knowledge, it was the first report of achieving 235g/L glucose in cellulose enzymatic hydrolysate derived from lignocellulose.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: liuyun@mail.buct.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Guo
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Liuyang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zhan
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
mesh_terms: D018533:Biomass; D002482:Cellulose / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000528:radiation effects; D008031:Lignin / Q000378:metabolism* / Q000528:radiation effects*; D009844:Oligosaccharides / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D014994:Xylose / Q000378:metabolism / Q000528:radiation effects
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; C036909:lignocellulose; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D014994:Xylose; D005947:Glucose
keywords: Enzymatic hydrolysis; High sugars concentration; Irradiation pretreatment; Lignocellulose; Two-step hydrolysis