title: A novel turn-on fluorescent strategy for sensing ascorbic acid using graphene quantum dots as fluorescent probe.
abstract: In this paper, a facile and rapid fluorescence turn-on assay for fluorescent detection of ascorbic acid (AA) was developed by using the orange emission graphene quantum dots (GQDs). In the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2), catechol can be oxidized by hydroxyl radicals and converted to o-benzoquinone, which can significantly quench the fluorescence of GQDs. However, when AA present in the system, it can consume part of H2O2and hydroxyl radicals to inhibit the generation of o-benzoquinone, resulting in fluorescence recovery. Under the optimized experimental conditions, the fluorescence intensity was linearly correlated with the concentration of H2O2in the range of 3.33-500µM with a detection limit of 1.2µM. The linear detection for AA was in the range from 1.11 to 300µM with a detection limit of 0.32µM. The proposed method was applied to the determination of AA in human serum samples with satisfactory results.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Weidan
authors.initials: W
authors.lastname: Na
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Ziping
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D001205:Ascorbic Acid / Q000097:blood*; D016227:Benzoquinones / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002396:Catechols / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D016227:Benzoquinones; D002396:Catechols; D005456:Fluorescent Dyes; D006108:Graphite; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C034221:catechol; D001205:Ascorbic Acid; C025225:2-benzoquinone
keywords: Ascorbic acid; Catechol; Graphene quantum dots (GQDs); H(2)O(2); O-benzoquinone