title: RABiT-II: Implementation of a High-Throughput Micronucleus Biodosimetry Assay on Commercial Biotech Robotic Systems.
abstract: We demonstrate the use of high-throughput biodosimetry platforms based on commercial high-throughput/high-content screening robotic systems. The cytokinesis-block micronucleus (CBMN) assay, using only 20 μl whole blood from a fingerstick, was implemented on a PerkinElmer cell::explorer and General Electric IN Cell Analyzer 2000. On average 500 binucleated cells per sample were detected by our FluorQuantMN software. A calibration curve was generated in the radiation dose range up to 5.0 Gy using the data from 8 donors and 48,083 binucleated cells in total. The study described here demonstrates that high-throughput radiation biodosimetry is practical using current commercial high-throughput/high-content screening robotic systems, which can be readily programmed to perform and analyze robotics-optimized cytogenetic assays. Application to other commercial high-throughput/high-content screening systems beyond the ones used in this study is clearly practical. This approach will allow much wider access to high-throughput biodosimetric screening for large-scale radiological incidents than is currently available.
authors.affiliation: a   Center for Radiological Research and.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Repin
authors.affiliation: b   Columbia Genome Center High-Throughput Screening facility, Columbia University Medical Center, New York, New York 10032.
authors.forename: Sergey
authors.initials: S
authors.lastname: Pampou
authors.affiliation: b   Columbia Genome Center High-Throughput Screening facility, Columbia University Medical Center, New York, New York 10032.
authors.forename: Charles
authors.initials: C
authors.lastname: Karan
authors.affiliation: a   Center for Radiological Research and.
authors.forename: David J
authors.initials: DJ
authors.lastname: Brenner
authors.affiliation: a   Center for Radiological Research and.
authors.forename: Guy
authors.initials: G
authors.lastname: Garty
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000528:radiation effects / Q000648:ultrastructure; D002138:Calibration; D005260:Female; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008297:Male; D015162:Micronucleus Tests / Q000295:instrumentation* / Q000379:methods; D008875:Middle Aged; D011874:Radiometry / Q000295:instrumentation / Q000379:methods; D012371:Robotics / Q000295:instrumentation* / Q000379:methods; D055815:Young Adult