title: Triterpene Constituents ofEuphorbia ErythradeniaBioss. and their Anti-HIV Activity.
abstract: Phytochemical investigation of the aerial parts ofEuphorbia erythradeniaBioss. (Euphorbiaceae), one of Iranian endemicEuphorbias, with particular attention to triterpene constituents, using methanol solvent extraction was carried out. Five known triterpenes, including four cycloartanes and oleanolic acid, were isolated for the first time and identified using NMR and Mass techniques. Anti HIV activity of the isolated triterpenes and ingenoid diterpenes was evaluated using single cycle replicable HIV-1 (SCR HIV-1) virions. Molecular features of the most active compound (IC50= 0.008 μM, CC50= 3.264 μM, TI = 380.64), which showed higher therapeutic index than nevirapine, was assessed using molecular docking. Docking studies demonstrated three hydrogen bonds between HIV-1 virion protease active site and this compound with a distance less than 3 A° which can be responsible for the observed anti HIV-1 activity.
authors.affiliation: Phytochemistry Research Center and School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abdul Majid
authors.initials: AM
authors.lastname: Ayatollahi
authors.affiliation: Phytochemistry Research Center and School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Zarei
authors.affiliation: Department of hepatitis and AIDS, Institute Pasture of Iran, Tehran, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Memarnejadian
authors.affiliation: Department of Pharmacognosy, Isfahan Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ghanadian
authors.affiliation: Department of Audiology, school of Rehabilitation, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Heydarian Moghadam
authors.affiliation: Department of Medicinal chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Kobarfard
keywords: Euphorbia erythradenia; NMR; anti HIV; docking; triterpene