title: Investigation of osteogenic activity of primary rabbit periosteal cells stimulated by multi-axial tensile strain.
abstract: Periosteum-derived cells was indicated to respond to mechanical force and have stem cell potential capable of differentiating into multiple tissue. Investigation of osteogenic activity under mechanical stimulation is important to understand the therapeutic conditions of fracture healing. In this work, a cell culture platform was developed for respectively providing isotropic and anisotropic axial strain. Primary rabbit periosteal cells were isolated and cultured in the chamber. Multi-axial tensile strain was received and osteogenic activity was investigated by mRNA expressions of CBFA1 and OPN. The highest mRNA expression was found in moderate strain (5-8%) under anisotropic axial strain. These results provided important foundation for further in vivo studies and development of tailor-made stretching rehabilitation equipment.
authors.affiliation: Bone and Joint Research Center, Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Tooyuan Branch, Taoyuan, Taiwan.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Chiu
authors.affiliation: Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Jun-Liang
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Chih-Hsuan
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan. kflei@mail.cgu.edu.tw.|Department of Mechanical Engineering, Chang Gung University, Taoyuan, Taiwan. kflei@mail.cgu.edu.tw.|Department of Radiation Oncology, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan, Taiwan. kflei@mail.cgu.edu.tw.
authors.forename: Kin Fong
authors.identifier: 0000-0002-5356-2221
authors.initials: KF
authors.lastname: Lei
authors.affiliation: Bone and Joint Research Center, Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Tooyuan Branch, Taoyuan, Taiwan. alvinchen@cgmh.org.tw.
authors.forename: Alvin Chao-Yu
authors.initials: AC
authors.lastname: Chen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D010012:Osteogenesis; D010521:Periosteum / Q000166:cytology* / Q000378:metabolism; D011312:Pressure; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013314:Stress, Mechanical; D013718:Tensile Strength
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger
keywords: Orthopedics; Osteogenic activity; Periosteal cells; Rehabilitation