title: Comparison of different extenders on the recovery and longevity of epididymal sperm from Spix's yellow-toothed cavies (Galea spixii Wagler, 1831).
abstract: The aim of this study was to evaluate the performance of cavy (Galea spixii) epididymal sperm following addition to TES or TRIS extenders and using a thermal resistance test (TRT), as well as fluorescence analysis as a complementary method to predict the viability of these gametes. Nine testicle-epididymis complexes were used for sperm collection using a flotation method. Epididymis tails were sliced and one was immersed in 3 ml of TRIS buffer, and the other in 3 ml of TES, for 5 min. After sperm recovery, the samples were subjected to a TRT which involved incubation in a water bath at 37°C for 3 h. During incubation, sample parameters were assessed at 0, 15, 30, 60, 90, 120, 150 or 180 min intervals. Results indicated that the TRIS diluent was more efficient than TES (P < 0.05) for the maintenance of sperm parameters in Spix's yellow-toothed cavies over the whole TRT, maintaining sperm longevity for an extended time. In conclusion, we indicate the use of TRIS diluent for recovery and maintenance of longevity of epididymal sperm from cavies (G. spixii).
authors.affiliation: Laboratório de Conservação e Germoplasma Animal,Universidade Federal Rural do Semi-Árido (UFERSA),Mossoró,RN,Brazil.
authors.forename: Andréia Maria
authors.initials: AM
authors.lastname: da Silva
authors.affiliation: Laboratório de Conservação e Germoplasma Animal,Universidade Federal Rural do Semi-Árido (UFERSA),Mossoró,RN,Brazil.
authors.forename: Patrícia Cunha
authors.initials: PC
authors.lastname: Sousa
authors.affiliation: Laboratório de Conservação e Germoplasma Animal,Universidade Federal Rural do Semi-Árido (UFERSA),Mossoró,RN,Brazil.
authors.forename: Lívia Batista
authors.initials: LB
authors.lastname: Campos
authors.affiliation: Laboratório de Conservação e Germoplasma Animal,Universidade Federal Rural do Semi-Árido (UFERSA),Mossoró,RN,Brazil.
authors.forename: José Artur Brilhante
authors.initials: JAB
authors.lastname: Bezerra
authors.affiliation: Laboratório de Conservação e Germoplasma Animal,Universidade Federal Rural do Semi-Árido (UFERSA),Mossoró,RN,Brazil.
authors.forename: Arthur Emannuel
authors.initials: AE
authors.lastname: de Araújo Lago
authors.affiliation: Laboratório de Conservação e Germoplasma Animal,Universidade Federal Rural do Semi-Árido (UFERSA),Mossoró,RN,Brazil.
authors.forename: Moacir Franco
authors.initials: MF
authors.lastname: de Oliveira
authors.affiliation: Departamento de Ciências Animais,Universidade Federal Rural do Semi-Árido (UFERSA),BR 110,Km 47,Bairro Pres. Costa e Silva. CEP: 59.625-900 Mossoró,RN,Brazil.
authors.forename: Alexandre Rodrigues
authors.initials: AR
authors.lastname: Silva
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004822:Epididymis / Q000166:cytology*; D006168:Guinea Pigs; D008136:Longevity / Q000187:drug effects*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*
chemical_list: D003451:Cryoprotective Agents
keywords: Fluorescent probe; Rodents; Sperm analysis; Thermal resistance; Wildlife