title: Roles of monocyte chemotactic protein 1 and nuclear factor-κB in immune response to spinal tuberculosis in a New Zealand white rabbit model.
abstract: This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) and nuclear factor kappa B (NF-κB) in immune response to spinal tuberculosis in a New Zealand white rabbit model. Forty-eight New Zealand white rabbits were collected and divided into four groups: experimental group (n=30, spinal tuberculosis model was established), the sham group (n=15, sham operation was performed) and the blank group (n=3). The qRT-PCR assay and western blotting were applied to detect the mRNA and protein expressions of MCP-1 and NF-κB in peripheral blood. ELISA was used to measure serum levels of MCP-1, NF-κB, IFN-γ, IL-2, IL-4, and IL-10. Flow cytometry was adopted to assess the distributions of CD4+, CD8+ lymphocytes and CD4+ CD25+ Foxp3 lymphocyte subsets. Compared with the sham and blank groups, the mRNA and protein expressions of MCP-1 and NF-κB in the experimental group were significantly increased. The experimental group had lower serum levels of IL-2 and IFN-γ and higher serum level of IL-10 than the sham and blank groups. In comparison to the sham and blank groups, CD4+ T lymphocyte subsets percentage, CD4+/CD8+ ratio and CD4+ CD25+ Foxp3+ Tregs subsets accounting for CD4+ lymphocyte in the experimental group were lower, while percentage of CD8+ T lymphocyte subsets was higher. Our study provided evidence that higher expression of MCP-1 and NF-κB may be associated with decreased immune function of spinal tuberculosis, which can provide a new treatment direction for spinal tuberculosis.
authors.affiliation: The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Guo
authors.affiliation: The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Bai
authors.affiliation: The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
authors.forename: B
authors.initials: B
authors.lastname: Qiang
authors.affiliation: Operating Room, the Fifth Hospital of Harbin, Harbin, China.
authors.forename: F H
authors.initials: FH
authors.lastname: Bu
authors.affiliation: The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
authors.forename: N
authors.initials: N
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018932:Chemokine CCL2 / Q000097:blood / Q000378:metabolism*; D016207:Cytokines / Q000097:blood / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007111:Immunity, Cellular / Q000276:immunology*; D008297:Male; D016328:NF-kappa B / Q000097:blood / Q000378:metabolism*; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D014399:Tuberculosis, Spinal / Q000276:immunology*
chemical_list: D018932:Chemokine CCL2; D016207:Cytokines; D016328:NF-kappa B