title: Evaluation of Cholesterol-lowering Activity of Standardized Extract ofMangifera indicain Albino Wistar Rats.
abstract: BACKGROUND
  Cholesterol lowering activity ofMangifera indicaL. has been determined by earlier researchers and kernel, leaf and bark have shown significant activity. However, the specific cholesterol lowering activity of leaf methanol extract has not been determined.
  
  
  METHODS
  The present study involved evaluation of cholesterol lowering potential of methanol extract ofM. indicaleaves using high cholesterol diet model in albino Wistar rats. The acute oral toxicity at a dose of 5000 mg/ kg body weight was also determined in female albino Wistar rats. Phytoconstituents Iriflophenone 3-C-β-D-glucoside and mangiferin were quantified in methanol extracts of different varieties of mango leaves using high performance liquid chromatography.
  
  
  CONCLUSIONS
  Significant cholesterol lowering activity was observed with methanol extract ofM. indicaleaves, at dose of 90 mg/kg body weight in rats and it was also found to be safe at dose of 5000 mg/kg rat body. Iriflophenone 3-C-β-D-glucoside and mangiferin were found to be in the range of 1.2 to 2.8% w/w and 3.9 to 4.6% w/w, respectively which along with 3 β taraxerol and other sterols could be contributing to the cholesterol lowering activity of mango leaves extract.
  
  
  CONCLUSIONS
  The phytosterols rich extract of Mangifera indica leaves is a good source of nutraceutical ingredient that have the potential to lower serum cholesterol levels.
  
  
  CONCLUSIONS
  TheMangifera indicaleaves methanolic extract showed significant cholesterol lowering activity in high cholesterol diet induced hypercholesterolaemia model in rats when evaluated at a dose of 90 mg/kg rat body weight. The extract was found to contain Iriflophenone 3-C-β-D-glucoside and mangiferin which along with 3 β taraxerol and other sterols could be contributing to the cholesterol lowering activity.
authors.affiliation: Research Scholar, Manipal University, Manipal, Bengaluru, Karnataka, India; Natural Remedies R and D Centre, Bengaluru, Karnataka, India.
authors.forename: G M
authors.initials: GM
authors.lastname: Gururaja
authors.affiliation: Natural Remedies R and D Centre, Bengaluru, Karnataka, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Mundkinajeddu
authors.affiliation: Natural Remedies R and D Centre, Bengaluru, Karnataka, India.
authors.forename: A Senthil
authors.initials: AS
authors.lastname: Kumar
authors.affiliation: Natural Remedies R and D Centre, Bengaluru, Karnataka, India.
authors.forename: Shekhar Michael
authors.initials: SM
authors.lastname: Dethe
authors.affiliation: Natural Remedies R and D Centre, Bengaluru, Karnataka, India.
authors.forename: J Joshua
authors.initials: JJ
authors.lastname: Allan
authors.affiliation: Natural Remedies R and D Centre, Bengaluru, Karnataka, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Agarwal
keywords: Hypercholesterolemia; Mangifera indica; mangiferin; mango leaves