title: Fluorescence Sensing of Inorganic Phosphate and Pyrophosphate Using Small Molecular Sensors and Their Applications.
abstract: The aim of this contribution is to provide an introduction and a brief summary of the principle of fluorescence molecular sensors specific to inorganic phosphate (Pi) and inorganic pyrophosphate (PPi) as well as their applications. In our introduction we describe the impact of both Pi and PPi in the living organism and in the environment, followed by a description of the principle of fluorescence molecular sensors and the sensing mechanism in solution. We then focus on exciting research which has emerged in recent years on the development of fluorescent sensors specific to Pi and PPi, categorized by chemical interactions between the sensor and the target molecule, such as hydrogen bonding, coordination chemistry, displacement assay, aggregation induced emission or quenching, and chemical reactions.
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok, 10400, Thailand.
authors.forename: Jirarut
authors.initials: J
authors.lastname: Wongkongkatep
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Ojida
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan. ihamachi@sbchem.kyoto-u.ac.jp.
authors.forename: Itaru
authors.initials: I
authors.lastname: Hamachi
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D010710:Phosphates / Q000032:analysis*; D054852:Small Molecule Libraries / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D010710:Phosphates; D054852:Small Molecule Libraries
keywords: Fluorescence detection; Imaging; Molecular sensor; Phosphate; Pyrophosphate