title: Effectoromics-Based Identification of Cell Surface Receptors in Potato.
abstract: In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.
authors.affiliation: Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Domazakis
authors.affiliation: Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Xiao
authors.initials: X
authors.lastname: Lin
authors.affiliation: Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Carolina
authors.initials: C
authors.lastname: Aguilera-Galvez
authors.affiliation: Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Doret
authors.initials: D
authors.lastname: Wouters
authors.affiliation: Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Gerard
authors.initials: G
authors.lastname: Bijsterbosch
authors.affiliation: Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Wolters
authors.affiliation: Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands. vivianne.vleeshouwers@wur.nl.
authors.forename: Vivianne G A A
authors.initials: VG
authors.lastname: Vleeshouwers
mesh_terms: D002874:Chromosome Mapping; D060467:Disease Resistance; D005838:Genotype; D055750:Phytophthora infestans / Q000472:pathogenicity*; D000069600:Plant Breeding; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism / Q000469:parasitology*
chemical_list: D010940:Plant Proteins; D051192:Receptors, Pattern Recognition
keywords: Agroinfiltration; Apoplastic effector; Effectoromics; Genetic mapping; PVX agroinfection; Pattern recognition receptors (PRRs); Protein infiltration; Solanum; Yeast protein production