title: [Establishment and Identification of SCL-tTA/BCR-ABL Transgenic Mouse Model with Chronic Myeloid Leukemia].
abstract: OBJECTIVE
  To establish transgenic mouse model with chronic myeloid leukemia(CML) by tetracycline withdrawal, so as to provide the experimental tool for studying pathogenesis of CML and searching new therapeutic method for this disease.
  
  
  METHODS
  The BCR-ABL expression in stem and progenitor cells in SCL-tTA/BCR-ABL transgenic mice was induced by tetracycline withdrawal in drinking water; and the peripheral hemogram and spleen index were examined after identifying genotypes in mice; the distribution of hematopoietic lineage in bone marrow, spleen and peripheral blood was detected by flow cytometry.
  
  
  RESULTS
  As compared with the control mice, the peripheral hemogram showed the obvious increase of neutrophile granulocyte count and spleen index in BCR-ABL transgenic mice. All induced double transgenic mice showed a severe myeloid cell expansion (Gr-1+Mac-1+) and reduction in T (CD3+) and B(B220+) cell lineages in bone marrow, spleen and peripheral blood.
  
  
  CONCLUSIONS
  A mouse model to induce BCR-ABL expression is successfully set up by tetracycline withdrawal, which resembles chronic phase CML.
authors.affiliation: Department of Pediatrics, Tangdu Hospital of The Fourth Military Medical University, Xi'an 710038, Shaanxi Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Hematology, The Forty-four Hospital of Chinese PLA, Guiyang 550009, Guizhou Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.affiliation: Department of Hematology, Tangdu Hospital of The Fourth Military Medical University, Xi'an 710038, Shaanxi Province, China. E-mail: liangym@fmmu.edu.cn.
authors.forename: Ying-Min
authors.initials: YM
authors.lastname: Liang
mesh_terms: D000818:Animals; D001853:Bone Marrow; D004195:Disease Models, Animal; D016044:Fusion Proteins, bcr-abl; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D016044:Fusion Proteins, bcr-abl