title: Identification of interacting proteins of the TaFVE protein involved in spike development in bread wheat.
abstract: WD-40 repeat-containing protein MSI4 (FVE)/MSI4 plays important roles in determining flowering time in Arabidopsis. However, its function is unexplored in wheat. In the present study, coimmunoprecipitation and nanoscale liquid chromatography coupled to MS/MS were used to identify FVE in wheat (TaFVE)-interacting or associated proteins. Altogether 89 differentially expressed proteins showed the same downregulated expression trends as TaFVE in wheat line 5660M. Among them, 62 proteins were further predicted to be involved in the interaction network of TaFVE and 11 proteins have been shown to be potential TaFVE interactors based on curated databases and experimentally determined in other species by the STRING. Both yeast two-hybrid assay and bimolecular fluorescence complementation assay showed that histone deacetylase 6 and histone deacetylase 15 directly interacted with TaFVE. Multiple chromatin-remodelling proteins and polycomb group proteins were also identified and predicted to interact with TaFVE. These results showed that TaFVE directly interacted with multiple proteins to form multiple complexes to regulate spike developmental process, e.g. histone deacetylate, chromatin-remodelling and polycomb repressive complex 2 complexes. In addition, multiple flower development regulation factors (e.g. flowering locus K homology domain, flowering time control protein FPA, FY, flowering time control protein FCA, APETALA 1) involved in floral transition were also identified in the present study. Taken together, these results further elucidate the regulatory functions of TaFVE and help reveal the genetic mechanisms underlying wheat spike differentiation.
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Zheng
authors.affiliation: Institute of Crop Sciences, National Key Facilities for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Lu
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Meng
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
authors.forename: Rong-Zhi
authors.initials: RZ
authors.lastname: Zhang
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
authors.forename: Jia-Mei
authors.initials: JM
authors.lastname: Sun
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
authors.forename: Mu-Mu
authors.initials: MM
authors.lastname: Wang
authors.affiliation: Institute of Crop Sciences, National Key Facilities for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Li
authors.affiliation: Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
authors.forename: Ru-Yu
authors.initials: RY
authors.lastname: Li
mesh_terms: D001681:Biological Assay; D002853:Chromatography, Liquid; D030562:Databases, Protein; D035264:Flowers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000378:metabolism*; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D010940:Plant Proteins
keywords: Co-IP; LC-MS/MS analysis; Spike development; TaFVE; Wheat