title: The role of alternative polyadenylation in the antiviral innate immune response.
abstract: Alternative polyadenylation (APA) is an important regulatory mechanism of gene functions in many biological processes. However, the extent of 3' UTR variation and the function of APA during the innate antiviral immune response are unclear. Here, we show genome-wide poly(A) sites switch and average 3' UTR length shortens gradually in response to vesicular stomatitis virus (VSV) infection in macrophages. Genes with APA and mRNA abundance change are enriched in immune-related categories such as the Toll-like receptor, RIG-I-like receptor, JAK-STAT and apoptosis-related signalling pathways. The expression of 3' processing factors is down-regulated upon VSV infection. When the core 3' processing factors are knocked down, viral replication is affected. Thus, our study reports the annotation of genes with APA in antiviral immunity and highlights the roles of 3' processing factors on 3' UTR variation upon viral infection.
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Shaochun
authors.initials: S
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yonggui
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yutong
authors.initials: Y
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Lan
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Peiyi
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.|School of Life Science, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China.
authors.forename: Anlong
authors.initials: A
authors.lastname: Xu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D007963:Leukocytes, Mononuclear; D008264:Macrophages; D051379:Mice; D008810:Mice, Inbred C57BL; D026723:Polyadenylation / Q000276:immunology*; D061251:Primary Cell Culture; D051192:Receptors, Pattern Recognition / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D020413:3' Untranslated Regions; D051192:Receptors, Pattern Recognition