title: What can mathematical modelling say about CHO metabolism and protein glycosylation?
abstract: Chinese hamster ovary cells have been in the spotlight for process optimization in recent years, due to being the major, long established cell factory for the production of recombinant proteins. A deep, quantitative understanding of CHO metabolism and mechanisms involved in protein glycosylation has proven to be attainable through the development of high throughput technologies. Here we review the most notable accomplishments in the field of modelling CHO metabolism and protein glycosylation.
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
authors.forename: Sarah N
authors.initials: SN
authors.lastname: Galleguillos
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
authors.forename: David
authors.initials: D
authors.lastname: Ruckerbauer
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
authors.forename: Matthias P
authors.initials: MP
authors.lastname: Gerstl
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
authors.forename: Nicole
authors.initials: N
authors.lastname: Borth
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Hanscho
authors.affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Zanghellini
keywords: 00-01; 13C-labelling; 99-00; CHO cells; CHO, Chinese hamster ovary; FBA, Flux Balance Analysis; GSMR, genome-scale metabolic reconstruction; Glycosylation; Kinetic model; MFA; MFA, metabolic flux analysis; Metabolic modelling; PPP, pentose phosphate pathway; TCA, tricarboxylic acid; mAb, monoclonal antibody