title: Storage of passive motion pattern in hippocampal CA1 region depends on CaMKII/CREB signaling pathway in a motion sickness rodent model.
abstract: Sensory mismatch between actual motion information and anticipated sensory patterns (internal model) is the etiology of motion sickness (MS). Some evidence supports that hippocampus might involve the neural storage of the "internal model". This study established an "internal model" acquisition-retention behavioral model using a repeated habituation rotation training protocol. We tried to identify the hippocampal subregion involved in "internal model" retention using chemical lesion methods. Hippocampal kinases (CaMK, CaMKIV, CREB and ERK1/2) phosphorylation in the target subregion was assayed and the effects of kinase inhibitors (KN93 or U0126) on "internal model" retention were investigated. The activities of potential kinases (CaMKII and CREB) were also examined in otoliths deficit het/het mice. In habituated rats, CA1 lesion reproduced MS-related behavioral responses on "internal model" retention day. Habituation training increased CaMKII and CREB activity but had no effect on CaMKIV and ERK1/2 activity in the CA1, while inhibition of CaMKII but not ERK1/2 impaired "internal model" retention. In het/het mice, CaMKII and CREB were not activated in the CA1 on the retention day. These results suggested that CaMKII/CREB pathway might potentially contribute to the storage of the "internal model" in the hippocampal CA1 after motion sickness induced by vestibular stimulation.
authors.affiliation: Department of Nautical Injury Prevention, Faculty of Navy Medicine, Shanghai, China.
authors.forename: Junqin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Nautical Injury Prevention, Faculty of Navy Medicine, Shanghai, China.
authors.forename: Jiluo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Nautical Injury Prevention, Faculty of Navy Medicine, Shanghai, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Nautical Injury Prevention, Faculty of Navy Medicine, Shanghai, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Qi
authors.affiliation: Department of Nautical Injury Prevention, Faculty of Navy Medicine, Shanghai, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Nautical Injury Prevention, Faculty of Navy Medicine, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Nautical Injury Prevention, Faculty of Navy Medicine, Shanghai, China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Cai
mesh_terms: D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D002070:Butadienes / Q000494:pharmacology; D056547:CA1 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D050882:CREB-Binding Protein / Q000235:genetics* / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009041:Motion Sickness / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009570:Nitriles / Q000494:pharmacology; D010037:Otolithic Membrane / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012399:Rotation; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D000096926:Benzenesulfonamides
chemical_list: D001596:Benzylamines; D002070:Butadienes; D050882:CREB-Binding Protein; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D009570:Nitriles; D013449:Sulfonamides; C113580:U 0126; C072105:KN 93; D000096926:Benzenesulfonamides; C494364:Crebbp protein, mouse; C517685:Camk4 protein, mouse; C535148:Mapk1 protein, mouse