title: PRC2 is required for extensive reorganization of H3K27me3 during epigenetic reprogramming in mouse fetal germ cells.
abstract: Defining how epigenetic information is established in the germline during fetal development is key to understanding how epigenetic information is inherited and impacts on evolution and human health and disease.
  
  
  
  Here, we show that Polycomb Repressive Complex 2 is transiently localized in the nucleus of mouse fetal germ cells, while DNA methylation is removed from the germline. This coincides with significant enrichment of trimethylated lysine 27 on histone 3 near the nuclear lamina that is dependent on activity of the essential PRC2 catalytic proteins, Enhancer of Zeste 1 and/or 2.
  
  
  
  Combined, these data reveal a role for Polycomb Repressive Complex 2 and trimethylated lysine 27 on histone 3 during germline epigenetic programming that we speculate is required to repress target sequences while DNA methylation is removed.
authors.affiliation: Department of Molecular and Translational Science, Centre for Genetic Diseases, Hudson Institute of Medical Research, Monash University, Clayton, VIC 3168 Australia.
authors.forename: Lexie
authors.initials: L
authors.lastname: Prokopuk
authors.affiliation: Department of Molecular and Translational Science, Centre for Genetic Diseases, Hudson Institute of Medical Research, Monash University, Clayton, VIC 3168 Australia.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Stringer
authors.affiliation: Department of Molecular and Translational Science, Centre for Genetic Diseases, Hudson Institute of Medical Research, Monash University, Clayton, VIC 3168 Australia.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hogg
authors.affiliation: Monash Micro Imaging, Monash University, Clayton, VIC 3800 Australia.
authors.forename: Kirstin D
authors.initials: KD
authors.lastname: Elgass
authors.affiliation: Department of Molecular and Translational Science, Centre for Genetic Diseases, Hudson Institute of Medical Research, Monash University, Clayton, VIC 3168 Australia.
authors.forename: Patrick S
authors.identifier: 0000-0002-7587-8227
authors.initials: PS
authors.lastname: Western
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000378:metabolism; D057890:Epigenomics; D005260:Female; D005333:Fetus / Q000166:cytology; D005854:Germ Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006066:Gonads / Q000378:metabolism / Q000473:pathology; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology; D008297:Male; D008745:Methylation; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D063151:Polycomb Repressive Complex 2 / Q000378:metabolism*; D011728:Pyridones / Q000494:pharmacology
chemical_list: C577920:GSK-2816126; D006657:Histones; D007211:Indoles; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D011728:Pyridones; D000071221:Enhancer of Zeste Homolog 2 Protein; C498408:Ezh1 protein, mouse; C498410:Ezh2 protein, mouse; D063151:Polycomb Repressive Complex 2
keywords: Epigenetic reprogramming; Epigenetics; Germ cells; H3K27me3; Histone modifications