title: Preparation of Photoirradiation Molecular Imprinting Polymer for Selective Separation of Branched Cyclodextrins.
abstract: In the present study, photoirradiation molecularly imprinted polymer (MIP) with azobenzene was used as a functional monomer for the selective separation of the branched cyclodextrins. The functional monomer 4-methacryloyloxy azobenzene (MAA) and the molecular template 6-O-α-d-maltosyl-β-cyclodextrin (G2-β-CD) were implemented for the molecular imprinting. The core-shell structure of photoirradiation MIP was visualized by the transmission electron microscopy (TEM). With Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), we identified that G2-β-CD was imprinted into the polymer and removed from the MIP. The binding association constant (Ka) and the maximum number of the binding site (Nmax) were 1.72 × 10⁴ M-1and 7.93 μmol·g-1MIP, respectively. With alternate irradiation at 365 and 440 nm light, the prepared MIP reversibly released and rebound to the G2-β-CD, which resulted in the nearly zero amount of G2-β-CD in the solution. The HPLC results indicated that the purity of G2-β-CD could reach 90.8% after going through MIP. The main finding of our study was that the photoirradiation of MIP was an easy and effective method for the selective separation of the branched cyclodextrins.
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. fanhaoran0622@163.com.|School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. fanhaoran0622@163.com.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. fanhaoran0622@163.com.
authors.forename: Haoran
authors.initials: H
authors.lastname: Fan
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. jpwang1984@jiangnan.edu.cn.|School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. jpwang1984@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. jpwang1984@jiangnan.edu.cn.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. mengqingranxzjscn@163.com.|School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. mengqingranxzjscn@163.com.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. mengqingranxzjscn@163.com.
authors.forename: Qingran
authors.initials: Q
authors.lastname: Meng
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. xmxubest@126.com.|School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. xmxubest@126.com.
authors.forename: Xueming
authors.initials: X
authors.lastname: Xu
authors.affiliation: Muyang Group Co., Ltd., Muyang Road, Yangzhou 225127, China. wangjinpeng1984@126.com.
authors.forename: Tianming
authors.initials: T
authors.lastname: Fan
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. fpcenter@jiangnan.edu.cn.|School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. fpcenter@jiangnan.edu.cn.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jin
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry*; D007536:Isomerism; D008027:Light; D008854:Microscopy, Electron; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D011827:Radiation; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D003505:Cyclodextrins; D011108:Polymers
keywords: azobenzene derivatives; branched cyclodextrins; molecular imprinting polymer; photoirradiation