title: Effects of Microcystis on Hypothalamic-Pituitary-Gonadal-Liver Axis in Nile Tilapia (Oreochromis niloticus).
abstract: In the present study, Nile tilapia (Oreochromis niloticus) were used to assess the endocrine disruption potential of Microcytis aeruginosa. Male Nile tilapia were exposed to lyophilized M. aeruginosa or purified microcystin-LR (8.3 μg/L) for 28 days. The levels of serum hormones (17β-estradiol and testosterone) and transcripts of selected genes in the hypothalamus-pituitary-gonadal-liver axis were analyzed. The results showed that serum hormones were significantly up-regulated, and transcripts of 13 genes (GHRH, PACAP, GH, GHR1, GHR2, IGF1, IGF2, CYP19a, CYP19b, 3β-HSD1, 20β-HSD, 17β-HSD1 and 17β-HSD8) were significantly altered after Microcytis exposure. These results indicate that fish reproduction can be altered in a Microcystis bloom-contaminated aquatic environment.
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.|Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, CAFS, Wuxi, 214081, China.
authors.forename: Jiazhang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.|Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, CAFS, Wuxi, 214081, China.
authors.forename: Shunlong
authors.initials: S
authors.lastname: Meng
authors.affiliation: School of Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China. xuhai@ujs.edu.cn.
authors.forename: Hai
authors.identifier: http://orcid.org/0000-0001-6549-1486
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases; D000818:Animals; D023681:Cichlids / Q000235:genetics; D052244:Endocrine Disruptors / Q000633:toxicity; D004958:Estradiol / Q000097:blood; D005612:Freeze Drying; D015870:Gene Expression / Q000187:drug effects*; D006066:Gonads / Q000187:drug effects*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008297:Male; D008387:Marine Toxins; D052998:Microcystins / Q000633:toxicity*; D046931:Microcystis / Q000737:chemistry*; D013739:Testosterone / Q000097:blood; D017210:Tilapia / Q000097:blood / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D052244:Endocrine Disruptors; D008387:Marine Toxins; D052998:Microcystins; D013739:Testosterone; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; C057862:cyanoginosin LR
keywords: Brain; Hypothalamus-pituitary-gonadal-liver axis; MC-LR; Microcystis; Nile tilapia