title: Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants.
abstract: Here we describe a method of gene mapping and cloning of chemical induced mutations using next-generation sequencing on their backcrossed segregants. This method utilizes polymorphisms induced by chemical mutagens for mapping and therefore does not require outcrossing to a different background or a large segregating population. It can be used to clone causal mutations generated in various accessions or mutant backgrounds for dissecting complex processes such as pattern recognition receptor pathways in plant immunity.
authors.affiliation: Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA. jh299@cornell.edu.
authors.forename: Jian
authors.initials: J
authors.lastname: Hua
authors.affiliation: Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Biocomputing Service Unit, Institute of Biotechnology, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods*; D018745:Genome, Plant / Q000187:drug effects; D059014:High-Throughput Nucleotide Sequencing; D007178:Inbreeding; D009153:Mutagens / Q000009:adverse effects; D009154:Mutation; D000073336:Whole Genome Sequencing
chemical_list: D009153:Mutagens
keywords: Cloning; Mapping; Mutagenesis; Next-generation sequencing