title: High-throughput quantification of the levels and labeling abundance of free amino acids by liquid chromatography tandem mass spectrometry.
abstract: Accurate assessment of mass isotopomer distributions (MIDs) of intracellular metabolites, such as free amino acids (AAs), is crucial for quantifying in vivo fluxes. To date, the majority of studies that measured AA MIDs have relied on the analysis of proteinogenic rather than free AAs by: i) GC-MS, which involved cumbersome process of derivatization, or ii) NMR, which requires large quantities of biological sample. In this work, the development and validation of a high-throughput LC-MS/MS method allowing the quantification of the levels and labeling of free AAs is described. Sensitivity in the order of the femtomol was achieved using multiple reaction monitoring mode (MRM). The MIDs of all free AAs were assessed without the need of derivatization, and were validated (except for Trp) on a mixture of unlabeled AA standards. Finally, this method was applied to the determination of the13C-labeling abundance in free AAs extracted from maize embryos cultured with13C-glutamine or13C-glucose. Although Cys was below the limit of detection in these biological samples, the MIDs of a total of 18 free AAs were successfully determined. Due to the increased application of tandem mass spectrometry for13C-Metabolic Flux Analysis, this novel method will enable the assessment of more complete and accurate labeling information of intracellular AAs, and therefore a better definition of the fluxes.
authors.affiliation: Department of Molecular Genetics, The Ohio State University, 1060 Carmack Road, Columbus, OH, 43210, USA; Center for Applied Plant Sciences, The Ohio State University, 1060 Carmack Road, Columbus, OH, 43210, USA.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Cocuron
authors.affiliation: Department of Molecular Genetics, The Ohio State University, 1060 Carmack Road, Columbus, OH, 43210, USA.
authors.forename: Enkhtuul
authors.initials: E
authors.lastname: Tsogtbaatar
authors.affiliation: Department of Molecular Genetics, The Ohio State University, 1060 Carmack Road, Columbus, OH, 43210, USA; Center for Applied Plant Sciences, The Ohio State University, 1060 Carmack Road, Columbus, OH, 43210, USA. Electronic address: alonso.19@osu.edu.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Alonso
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002247:Carbon Isotopes / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes
keywords: (13)C-labeling; Amino acids; LC–MS/MS; Mass isotopomer distribution; Metabolic flux analysis; Multiple reaction monitoring