title: [Proteome analysis of undiluted vitreous humor in patients with branch retinal vein occlusion].
abstract: The pathophysiological mechanisms of macular edema secondary to branch retinal vein occlusion (BRVO) remain unclear.
  
  
  
  To analyze the protein profile of human vitreous of patients with BRVO and to identify specific dysregulated proteins.
  
  
  
  Undiluted vitreous humor samples from patients with treatment naïve BRVO and 15 controls with idiopathic floaters were analyzed in this clinical-experimental study using capillary electrophoresis coupled to a mass spectrometer (CE-MS) and tandem mass spectrometry (MS/MS). Quantitative analysis of the dysregulated proteins was performed with enzyme-linked immunosorbent assay (ELISA). Protein-protein interactions were depicted with the STRING database.
  
  
  
  A total of 84 proteins were found in the human vitreous samples of 15 patients with BRVO and 15 controls. In all, 14 proteins were significant when comparing the signal intensities of BRVO and control samples. Six significant dysregulated proteins with p < 0.001 were further verified with ELISA. Clusterin, complement factor C3, prostaglandin-H2 D‑isomerase and vitronectin were significantly upregulated in the BRVO group and opticin was downregulated. The protein interactions analysis showed associations with inflammatory cascades, matrix changes, mechanisms of cell survival und death.
  
  
  
  The results of the study reveal that the proteomic composition of vitreous humor differed significantly between the patients with BRVO and the controls. Whether the identified proteins may serve as potential biomarkers for pathophysiology, diagnostics or therapy should be examine in further studies.
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.
authors.forename: I
authors.initials: I
authors.lastname: Dacheva
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.|Augenklinik, Albert-Ludwigs-University Freiburg, Freiburg, Deutschland.
authors.forename: M
authors.initials: M
authors.lastname: Reich
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.
authors.forename: M
authors.initials: M
authors.lastname: Nobl
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.|Medical Research Centre, Polish Academy of Science, Warsaw, Polen.
authors.forename: K
authors.initials: K
authors.lastname: Ceglowska
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.
authors.forename: J
authors.initials: J
authors.lastname: Wasiak
authors.affiliation: Mosaiques Diagnostics GmbH, Hannover, Deutschland.
authors.forename: J
authors.initials: J
authors.lastname: Siwy
authors.affiliation: Mosaiques Diagnostics GmbH, Hannover, Deutschland.
authors.forename: P
authors.initials: P
authors.lastname: Zürbig
authors.affiliation: Mosaiques Diagnostics GmbH, Hannover, Deutschland.|BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK.
authors.forename: H
authors.initials: H
authors.lastname: Mischak
authors.affiliation: Augenklinik, Goethe-Universität, Frankfurt am Main, Deutschland.
authors.forename: F H J
authors.initials: FHJ
authors.lastname: Koch
authors.affiliation: Pathologisches Institut, Universität Heidelberg, Heidelberg, Deutschland.
authors.forename: J
authors.initials: J
authors.lastname: Kopitz
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.
authors.forename: F T A
authors.initials: FTA
authors.lastname: Kretz
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.
authors.forename: T
authors.initials: T
authors.lastname: Tandogan
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland.
authors.forename: G U
authors.initials: GU
authors.lastname: Auffarth
authors.affiliation: Universitätsaugenklinik Heidelberg, Im Neuenheimer Feld 400, 69120, Heidelberg, Deutschland. michael.koss@me.com.|Department of Ophthalmology, University of Southern California, Los Angeles, CA, USA. michael.koss@me.com.
authors.forename: M J
authors.initials: MJ
authors.lastname: Koss
mesh_terms: D006801:Humans; D008269:Macular Edema; D020543:Proteome; D040901:Proteomics; D012170:Retinal Vein Occlusion; D053719:Tandem Mass Spectrometry; D042461:Vascular Endothelial Growth Factor A; D014822:Vitreous Body
chemical_list: D020543:Proteome; D042461:Vascular Endothelial Growth Factor A
keywords: Branch retinal vein occlusion; Mass spectrometry; Proteomics; VEGF; Vitreous humor