title: Conformational dynamics of the rotary subunit F in the A3B3DF complex of Methanosarcina mazei Gö1 A-ATP synthase monitored by single-molecule FRET.
abstract: In archaea the A1AOATP synthase uses a transmembrane electrochemical potential to generate ATP, while the soluble A1domain (subunits A3B3DF) alone can hydrolyse ATP. The three nucleotide-binding AB pairs form a barrel-like structure with a central orifice that hosts the rotating central stalk subunits DF. ATP binding, hydrolysis and product release cause a conformational change inside the A:B-interface, which enforces the rotation of subunits DF. Recently, we reported that subunit F is a stimulator of ATPase activity. Here, we investigated the nucleotide-dependent conformational changes of subunit F relative to subunit D during ATP hydrolysis in the A3B3DF complex of the Methanosarcina mazei Gö1 A-ATP synthase using single-molecule Förster resonance energy transfer. We found two conformations for subunit F during ATP hydrolysis.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Dhirendra
authors.initials: D
authors.lastname: Singh
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.|Single-Molecule Microscopy Group, Jena University Hospital, Germany.
authors.forename: Hendrik
authors.identifier: http://orcid.org/0000-0001-5231-7286
authors.initials: H
authors.lastname: Sielaff
authors.affiliation: Single-Molecule Microscopy Group, Jena University Hospital, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Börsch
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Grüber
mesh_terms: D025181:ATP Synthetase Complexes / Q000737:chemistry / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006868:Hydrolysis; D007700:Kinetics; D017020:Methanosarcina / Q000201:enzymology*; D018613:Microscopy, Confocal; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D011487:Protein Conformation; D000072417:Protein Domains; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D021122:Protein Subunits; D000255:Adenosine Triphosphate; D025181:ATP Synthetase Complexes
keywords: ATP synthase; Förster resonance energy transfer; Methanosarcina mazei Gö1; bioenergetics; single molecule; subunit F