title: Morus alba leaf extract mediates neuroprotection against glyphosate-induced toxicity and biochemical alterations in the brain.
abstract: Recent studies demonstrate that glyphosate exposure is associated with oxidative stress and some neurological disorders such as Parkinson's pathology. Therefore, phytochemicals, in particular phenolic compounds, have attracted increasing attention as potential agents for neuroprotection. In the present study, we investigate the impact of glyphosate on the rat brain following i.p. injection and the possible molecular target of neuroprotective activity of the phenolic fraction from Morus alba leaf extract (MALE) and its ability to reduce oxidative damage in the brain. Wistar rats from 180 to 240 g were i.p. treated with a single dose of glyphosate (100 mg kg-1b.w.) or MALE (100 μg mL-1kg-1b.w.) for 2 weeks. Brain homogenates were used to evaluate neurotoxicity induced by the pesticide. For this, biochemical parameters were measured. Data shows that MALE regulated oxidative stress and counteracted glyphosate-induced deleterious effects and oxidative damage in the brain, as it abrogated LDH, protein carbonyls, and malonyldialdehyde. MALE also appears to be able to scavenge H2O2levels, maintain iron and Ca2+homeostasis, and increase SOD activity. Thus, in vivo results showed that mulberry leaf extract is a potent protector against glyphosate-induced toxicity, and its protective effect could result from synergism or antagonism between the various bioactive phenolic compounds in the acetonic fraction from M. alba leaf extract.
authors.affiliation: Research Unit of Functional Neurophysiology and Pathology, 00/UR/08-01, Faculty of Science of Tunis, University El Manar, 2092, Tunis, Tunisia. olfa.rebai@yahoo.fr.
authors.forename: Olfa
authors.initials: O
authors.lastname: Rebai
authors.affiliation: Research Unit of Functional Neurophysiology and Pathology, 00/UR/08-01, Faculty of Science of Tunis, University El Manar, 2092, Tunis, Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Belkhir
authors.affiliation: Laboratory LIP-MB, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Tunis, Tunisia.
authors.forename: Adnen
authors.initials: A
authors.lastname: Boujelben
authors.affiliation: Laboratory LIP-MB, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Tunis, Tunisia.
authors.forename: Sami
authors.initials: S
authors.lastname: Fattouch
authors.affiliation: Research Unit of Functional Neurophysiology and Pathology, 00/UR/08-01, Faculty of Science of Tunis, University El Manar, 2092, Tunis, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Amri
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects; D005998:Glycine / Q000031:analogs & derivatives; D006861:Hydrogen Peroxide; D031625:Morus / Q000737:chemistry*; D000066829:Neuroprotection; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006861:Hydrogen Peroxide; D010936:Plant Extracts; D000097797:Glyphosate
keywords: Biochemical parameters; Glyphosate; Morus alba; Neurotoxicity