title: Circulating mircoRNA-21 as a predictor for vascular restenosis after interventional therapy in patients with lower extremity arterial occlusive disease.
abstract: The present study was designed to investigate the role of circulating miRNA-21 (miR-21) in vascular restenosis of lower extremity arterial occlusive disease (LEAOD) patients after interventional therapy. A total of 412 LEAOD patients were enrolled randomly in the present study. According to computed tomography angiography (CTA) and ankle-brachial index (ABI), patients were assigned into the restenosis group and the non-restenosis group.miR-21expression was detected with quantitative real-time PCR (qRT-PCR) before and after patients underwent interventional therapy. A follow-up period of 6 months was achieved. A receiver operating characteristic (ROC) curve was drawn and the area under the curve (AUC) was calculated to assess the predictive value ofmiR-21in vascular restenosis. Patients were older in the restenosis group than in the non-restenosis group. The percentages of patients with diabetes and hypertension were higher in the restenosis group than in the non-restenosis group, and the Fontaine stage exhibited a significant difference between the two groups.miR-21expression was higher in the restenosis group than in the non-restenosis group.miR-21expression level was related to age, diabetes and hypertension in the restenosis group. UsingmiR-21to predict vascular restenosis yielded an AUC of 0.938 (95% confidence interval (CI): 0.898-0.977), with Youden index of 0.817, sensitivity of 83.5% and specificity of 98.2%. Logistic regression analysis revealed that diabetes andmiR-21expression were the major risk factors for vascular restenosis of LEAOD.miR-21can be used as a predictive indicator for vascular restenosis of LEAOD after interventional therapy.
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China haiyang_wa@163.com qingfeng_hlj@126.com.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Sun
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Si-Qi
authors.initials: SQ
authors.lastname: Liu
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Bao
authors.initials: B
authors.lastname: Jing
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Jiao
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
authors.forename: Hao-Cheng
authors.initials: HC
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China haiyang_wa@163.com qingfeng_hlj@126.com.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D001157:Arterial Occlusive Diseases / Q000097:blood* / Q000473:pathology* / Q000628:therapy; D005260:Female; D005500:Follow-Up Studies; D006083:Graft Occlusion, Vascular / Q000097:blood* / Q000473:pathology*; D006801:Humans; D035002:Lower Extremity / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D012372:ROC Curve; D012307:Risk Factors
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: Interventional therapy; Lower extremity arterial occlusive disease; Sensitivity; Specificity; Vascular restenosis; the First Affiliated Hospital of Harbin Medical University