title: Extracellular Matrix Proteome and Phosphoproteome of Potato Reveals Functionally Distinct and Diverse Canonical and Non-Canonical Proteoforms.
abstract: The extracellular matrix (ECM) has a molecular machinery composed of diverse proteins and proteoforms that combine properties of tensile strength with extensibility exhibiting growth-regulatory functions and self- and non-self-recognition. The identification of ECM proteoforms is the prerequisite towards a comprehensive understanding of biological functions accomplished by the outermost layer of the cell. Regulatory mechanisms of protein functions rely on post-translational modifications, phosphorylation in particular, affecting enzymatic activity, interaction, localization and stability. To investigate the ECM proteoforms, we have isolated the cell wall proteome and phosphoproteome of a tuberous crop, potato (Solanum tuberosum). LC-MS/MS analysis led to the identification of 38 proteins and 35 phosphoproteins of known and unknown functions. The findings may provide a better understanding of biochemical machinery and the integrated protein and phosphoprotein network of ECM for future functional studies of different developmental pathways and guidance cues in mechanosensing and integrity signaling.
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. schakraborty@nipgr.ac.in.
authors.forename: Eman
authors.initials: E
authors.lastname: Elagamey
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. gayatrinarula@gmail.com.
authors.forename: Kanika
authors.initials: K
authors.lastname: Narula
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. arunima35@gmail.com.
authors.forename: Arunima
authors.initials: A
authors.lastname: Sinha
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. aggarwal.pooja28@yahoo.com.
authors.forename: Pooja Rani
authors.initials: PR
authors.lastname: Aggarwal
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. sgblackpearl@gmail.com.
authors.forename: Sudip
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. nchakraborty@nipgr.ac.in.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. subhrac@hotmail.com.
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
keywords: 2-DE; ECM; phosphoproteome; potato; protein interaction network; proteoforms