title: Expression of nestin and chromogranin in regeneration zones of rat pancreas.
abstract: OBJECTIVE
  The particular signals that start and orchestrate the regeneration process in pancreas are not well understood yet. We aimed to investigate the expression of nestin and chromogranin A in pancreatic regeneration zones and a secondary objective, we assessed the efficiency of pancreatic duct ligation method in creation of a pancreatic regeneration model in rats.
  
  
  METHODS
  Partial (90%) pancreatectomy and pancreatic duct ligation were performed in Wistar rats, in order to create pancreatic regeneration models. Pancreatic tissues were examined histologically. Expression profiles were investigated by immunohistochemistry for nestin and chromogranin A.
  
  
  RESULTS
  Nestin and chromogranin A expressions were observed in regeneration zones. Pancreatic regenerations zones were seen in pancreatic duct ligation group samples as well as partial pancreatectomy group. Nestin was expressed prominently in acinoductular metaplasia cells in regeneration zones. This was best demonstrated in the samples of pancreatic duct ligation group. In the subsequent sections of nestin positive sites, cytoplasmic positivity with chromogranin A was observed.
  
  
  CONCLUSIONS
  This study confirms that nestin and chromogranin A can be detected in neogenesis-evoked pancreatic tissue, particularly in the acinoductular epithelium. Nestin and chromogranin A may be important markers to identify pancreatic stem cells. Pancreatic duct ligation can be used for creating pancreatic regeneration model in rats.
  
  
  BACKGROUND
  Chromogranin A, Nestin, Pancreas, Regeneration, Stem cells.
authors.forename: Bahadir
authors.initials: B
authors.lastname: Ege
authors.forename: Tolga
authors.initials: T
authors.lastname: Dinc
authors.forename: Selami Ilgaz
authors.initials: SI
authors.lastname: Kayilioglu
authors.forename: Ekmel
authors.initials: E
authors.lastname: Tezel
authors.forename: Emin
authors.initials: E
authors.lastname: Ersoy
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002864:Chromogranins / Q000378:metabolism*; D004195:Disease Models, Animal; D008026:Ligation; D008297:Male; D064231:Nestin / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism; D010180:Pancreatectomy; D010183:Pancreatic Ducts / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D002864:Chromogranins; D064231:Nestin