title: Safety evaluation of soybean protein isolate oxidized by a hydroxyl radical-generating system.
abstract: The oxidative modification of soybean protein isolate (SPI) induced by a hydroxyl radical-generating system (HRGS) has a broad range of applications. However, few toxicology studies exist on this material. The safety of HRGS-oxidized SPI was assessed using subchronic and genotoxicity studies. A 30-day subchronic study (250, 500 and 1000 mg/kg∙BW) in rats showed no significant adverse effects on food consumption, body weight (BW), mortality, hematology, biochemistry, necropsy, organ weight or histopathology. The result of an Ames test showed that HRGS-oxidized SPI was not mutagenic to the test strains. The results of a bone marrow micronucleus test and mouse sperm abnormality test showed HRGS-oxidized SPI (417.5, 835.0 and 1670.0 mg/kg⋅BW) did not produce any aberrant effects on bone marrow cells or mouse sperm. Therefore, HRGS-oxidized SPI showed no genotoxicity in vivo or in vitro. In conclusion, these results support the safe use of HRGS-oxidized SPI as a food and dietary supplement.
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Hao
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Sun
authors.affiliation: Tongjiang Entry-Exit Inspection and Quarantine Bureau, Tongjiang, Heilongjiang 156400, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address: shml0915@163.com.
authors.forename: Meili
authors.initials: M
authors.lastname: Shao
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005260:Female; D017665:Hydroxyl Radical / Q000737:chemistry*; D008297:Male; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests / Q000379:methods; D009152:Mutagenicity Tests / Q000379:methods*; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins / Q000737:chemistry / Q000633:toxicity*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D059550:Toxicity Tests, Subchronic
chemical_list: D030262:Soybean Proteins; D017665:Hydroxyl Radical
keywords: Genotoxicity; Oxidative modification; Safety evaluation; Soybean protein isolate; Subchronic study