title: TheArabidopsiskinesin-4, FRA1, requires a high level of processive motility to function correctly.
abstract: Processivity is important for kinesins that mediate intracellular transport. Structure-function analyses of N-terminal kinesins (i.e. kinesins comprising their motor domains at the N-terminus) have identified several non-motor regions that affect processivityin vitroHowever, whether these structural elements affect kinesin processivity and functionin vivois not known. Here, we used anArabidopsis thalianakinesin-4, called Fragile Fiber 1 (FRA1, also known as KIN4A), which is thought to mediate vesicle transport, to test whether mutations that alter processivityin vitrolead to similar changes in behaviorin vivoand whether processivity is important for the function of FRA1. We generated several FRA1 mutants that differed in their 'run lengths'in vitroand then transformed them into thefra1-5mutant for complementation andin vivomotility analyses. Our data show that the behavior of processivity mutantsin vivocan differ dramatically fromin vitroproperties, underscoring the need to extend structure-function analyses of kinesinsin vivoIn addition, we found that a high density of processive motility is necessary for the physiological function of FRA1.
authors.affiliation: Biology Department, Washington University in St. Louis, St. Louis, MO 63130, USA.
authors.forename: Anindya
authors.initials: A
authors.lastname: Ganguly
authors.affiliation: Biology Department, Washington University in St. Louis, St. Louis, MO 63130, USA.
authors.forename: Logan
authors.initials: L
authors.lastname: DeMott
authors.affiliation: Biology Department, Washington University in St. Louis, St. Louis, MO 63130, USA ramdixit@wustl.edu.
authors.forename: Ram
authors.identifier: 0000-0001-7881-2859
authors.initials: R
authors.lastname: Dixit
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D020868:Gene Silencing; D016547:Kinesins; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010766:Phosphorylation; D000072417:Protein Domains; D012694:Serine / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C472140:FRA1 protein, Arabidopsis; D008869:Microtubule-Associated Proteins; D050505:Mutant Proteins; D012694:Serine; D016547:Kinesins
keywords: Intracellular transport; Kinesin; Live imaging; Motor protein; Single molecule