title: Genome-wide analysis of the HD-ZIP IV transcription factor family in Gossypium arboreum and GaHDG11 involved in osmotic tolerance in transgenic Arabidopsis.
abstract: HD-ZIP IV proteins belong to the homeodomain-leucine zipper (HD-ZIP) transcription factor family and are involved in trichome development and drought stress in plants. Although some functions of the HD-ZIP IV group are well understood in Arabidopsis, little is known about their function in cotton. In this study, HD-ZIP genes were identified from three Gossypium species (G. arboreum, G. raimondii and G. hirsutum) and clustered into four families (HD-ZIP I, II, III and IV) to separate HD-ZIP IV from the other three families. Systematic analyses of phylogeny, gene structure, conserved domains, and expression profiles in different plant tissues and the expression patterns under osmotic stress in leaves were further conducted in G. arboreum. More importantly, ectopic overexpression of GaHDG11, a representative of the HD-ZIP IV family, confers enhanced osmotic tolerance in transgenic Arabidopsis plants, possibly due to elongated primary root length, lower water loss rates, high osmoprotectant proline levels, significant levels of antioxidants CAT, and/or SOD enzyme activity with reduced levels of MDA. Taken together, these observations may lay the foundation for future functional analysis of cotton HD-ZIP IV genes to unravel their biological roles in cotton.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.|Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Eryong
authors.initials: E
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.|College of Agronomy, Xinjiang Agricultural University, Urumqi, 830052, China.
authors.forename: Zhaoen
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Zuoren
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Depei
authors.initials: D
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Hamama Islam
authors.initials: HI
authors.lastname: Butt
authors.affiliation: Huazhong Agricultural University, Wuhan, 430070, China. xlzhang@mail.hzau.edu.cn.
authors.forename: Xianlong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China. aylifug@163.com.
authors.forename: Fuguang
authors.initials: F
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D002374:Catalase / Q000378:metabolism; D002874:Chromosome Mapping; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D055106:Genome-Wide Association Study; D003368:Gossypium / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D016350:Leucine Zippers / Q000235:genetics*; D009997:Osmotic Pressure / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics; D013482:Superoxide Dismutase / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; C493946:HD-Zip protein, Arabidopsis; C586313:HDG11 protein, Arabidopsis; D018398:Homeodomain Proteins; D014157:Transcription Factors; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Arabidopsis; GaHDG11; Gossypium arboreum; HD-ZIP IV transcription factor; Osmotic tolerance