title: Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium.
abstract: Interferon tau (IFN-τ) is a ruminant-specific type I IFN secreted by a conceptus before its attachment to the uterus. IFN-τ induces the expression of IFN-stimulated genes (ISGs) via the type I IFN receptor (IFNAR), which is composed of IFNAR1 and IFNAR2 subunits in the endometrium. However, expression patterns of IFNARs during the estrous cycle have not been reported. We hypothesized that the response to a type I IFN changes along with IFNARs and the IFN-regulatory factors (IRFs) driving transcription of IFN signal-related genes and modulating a type I IFN signal during the estrous cycle. We investigated the estrous cycle stage-dependent type I IFN induction of ISGs and expression patterns of IFN signal-related genes in bovine endometrial tissues. Endometrial tissue pieces collected from bovine uteri at each estrous stage (early, mid, and late) were cultured with or without recombinant bovine IFN-α or concentrated pregnant uterine flushing (PUF) on day 18 after confirming the presence of a conceptus. IFN-α and PUF each significantly increased the expression of ISGs in endometrial tissues. The induction levels of the typical ISGs (MX1-a and ISG15) were significantly higher at the mid stage and correlated with high expression of IRFs at the mid stage. The immunostaining of IFNARs showed strong fluorescence intensities in luminal and glandular epithelia at the early and mid stages. Collectively, these results suggest that the endometrium exhibits estrous cycle stage-dependent responsiveness to type I IFN that may be associated with the expression of IFNARs and IRFs for pregnancy recognition.
authors.affiliation: Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shirozu
authors.affiliation: Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Iwano
authors.affiliation: Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Ogiso
authors.affiliation: Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Ahmed Z
authors.initials: AZ
authors.lastname: Balboula
authors.affiliation: Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Hanako
authors.initials: H
authors.lastname: Bai
authors.affiliation: Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawahara
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Ibaraki 305-8517, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Laboratory of Animal Breeding, Veterinary Medical Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Bai
authors.initials: B
authors.lastname: Rulan
authors.affiliation: National Institute of Animal Science, Animal Genetic Resources Research Center, Namwon 590-832, Republic of Korea.
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Yanagawa
authors.affiliation: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Nagano
authors.affiliation: Laboratory of Animal Breeding, Veterinary Medical Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Imakawa
authors.affiliation: Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D002417:Cattle; D004717:Endometrium / Q000378:metabolism*; D030762:Estrous Cycle / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D007370:Interferon Type I / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D007370:Interferon Type I
keywords: Cow; Interferon-stimulated gene (ISG); Type I Interferon (IFN); Type I interferon receptor (IFNAR); Uterus