title: Macrophage infiltration of electrospun polyester fibers.
abstract: Electrospun fibrous polylactide (PLA) membranes have been widely used for peritendinous anti-adhesion, but their degradation may lead to granuloma formation triggered by macrophages as the result of a foreign body reaction. To understand and solve this problem in peritendinous anti-adhesion, the effects of electrospun fibrous PLA membranes (PLA-M) and the mechanism by which macrophages elicit a foreign body reaction should be elaborated. Thus, the purpose of this study was to evaluate the ability of PLA-M and ibuprofen (IBU)-loaded fibrous PLA membranes (IBU/PLA-M) to prevent adhesion/granuloma formation around the tendon, and to understand the mechanism of macrophage infiltration. The results showed that PLA-M and IBU/PLA-M were porous and that IBU could be sustainably released from IBU/PLA-M. The adhesion and proliferation of RAW264.7 macrophages were worse on the surface of IBU/PLA-M than on PLA-M. After implantation around the tendon, IBU/PLA-M had higher anti-adhesion and tendon healing scores than PLA-M. Furthermore, PLA-M was able to promote macrophage infiltration, neovascularization, TNF-α expression and collagen III deposition to a greater extent than IBU/PLA-M. Consequently, these results show that macrophages can be triggered by PLA-M which subsequently leads to inflammation and granuloma formation, while IBU/PLA-M results in enhanced anti-inflammation and anti-adhesion effects when compared to PLA-M, by reducing macrophage infiltration.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China. wgcui80@hotmail.com xuyaozeng@163.com and Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, P.R. China.
authors.forename: Shen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China. wgcui80@hotmail.com xuyaozeng@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, P.R. China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China. wgcui80@hotmail.com xuyaozeng@163.com.
authors.forename: Guoqing
authors.identifier: http://orcid.org/0000-0001-5187-796X
authors.initials: G
authors.lastname: Pan
authors.affiliation: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, P.R. China.
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China. wgcui80@hotmail.com xuyaozeng@163.com.
authors.forename: Yaozeng
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China. wgcui80@hotmail.com xuyaozeng@163.com.
authors.forename: Wenguo
authors.identifier: http://orcid.org/0000-0002-6938-9582
authors.initials: W
authors.lastname: Cui
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D004560:Electricity; D006099:Granuloma / Q000276:immunology / Q000378:metabolism; D007052:Ibuprofen / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D008567:Membranes, Artificial; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D013710:Tendons / Q000276:immunology
chemical_list: D004337:Drug Carriers; D008567:Membranes, Artificial; D011091:Polyesters; C033616:poly(lactide); D007052:Ibuprofen