title: The origin and evolution of Basigin(BSG) gene: A comparative genomic and phylogenetic analysis.
abstract: Basigin (BSG), also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or cluster of differentiation 147 (CD147), plays various fundamental roles in the intercellular recognition involved in immunologic phenomena, differentiation, and development. In this study, we aimed to compare the similarities and differences of BSG among organisms and explore possible evolutionary relationships based on the comparison result. We used the extensive BLAST tool to search the metazoan genomes, N-glycosylation sites, the transmembrane region and other functional sites. We then identified BSG homologs from genomic sequences and analyzed their phylogenetic relationships. We identified that BSG genes exist not only in the vertebrate metazoans but also in the invertebrate metazoans such as Amphioxus B. floridae, D. melanogaster, A. mellifera, S. japonicum, C. gigas, and T. patagoniensis. After sequence analysis, we confirmed that only vertebrate metazoans and Cephalochordate (amphioxus B. floridae) have the classic structure (a signal peptide, two Ig-like domains (IgC2 and IgI), a transmembrane region, and an intracellular domain). The invertebrate metazoans (excluding amphioxus B. floridae) lack the N-terminal signal peptides and IgC2 domain. We then generated a phylogenetic tree, genome organization comparison, and chromosomal disposition analysis based on the biological information obtained from the NCBI and Ensembl databases. Finally, we established the possible evolutionary scenario of the BSG gene, which showed the restricted exon rearrangement that has occurred during evolution, forming the present-day BSG gene.
authors.affiliation: Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200062, China.
authors.forename: Shenglan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Organ Transplant Center, Xiangya 3rd Hospital of Central South University, Changsha, Hunan 410013, China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Shao
authors.affiliation: Marine Biotechnology Research Center, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, Institute of Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China. Electronic address: liufeiguo2010@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D005075:Biological Evolution; D019295:Computational Biology; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D016678:Genome; D007136:Immunoglobulins / Q000235:genetics; D007448:Invertebrates / Q000276:immunology*; D064175:Lancelets / Q000276:immunology*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D007136:Immunoglobulins; D051926:Basigin
keywords: Basigin (BSG); Comparative genomics; Evolution; Ig superfamily