title: Synergy of Raddeanin A and cisplatin induced therapeutic effect enhancement in human hepatocellular carcinoma.
abstract: Cisplatin is a main compound for human hepatocellular carcinoma (HCC) chemotherapies, but it has certain cytotoxicity during applications. To release that, combining with other drugs are being as a regular plan in clinic. In our present study, we are focusing on one of active monomers extracted from Anemone Raddeana Regel, Raddeanin A (RA), which is on behalf of the same character like cisplatin in the tumor remedies. In order to investigate whether combination usage of RA and cisplatin can be priority to the later drug's effect development and its toxicity reduction in HCC, both of two drugs were treated 24 h or 48 h in QGY-7703 cells for estimating their abilities in tumor cell proliferation inhibition. Results show RA makes synergistic functions with cisplatin after measuring and analyzing their combination index (CI) values. Meanwhile it can strengthen cisplatin's effect through arresting the tumor cells in G0/G1 cycle and further promoting their apoptosis. Interestingly, the molecule signals correlated to tumor cell apoptosis containing both of p53 and bax are simultaneously activated, but bcl-2 and survivin are all depressed in mRNA level. Meanwhile, combining usage with RA can even raise the intracellular productions of reactive oxygen species (ROS). All these consequences reflect RA plays an important role in enhancing the therapeutic effect of cisplatin in HCC. This finding may guide for the drug usage of cisplatin in clinic practice.
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Analysis, School of Pharmaceutical Sciences, Changchun University of Traditional Chinese Medicine, Changchun 130117, China.
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Li
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Analysis, School of Pharmaceutical Sciences, Changchun University of Traditional Chinese Medicine, Changchun 130117, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Analysis, School of Pharmaceutical Sciences, Changchun University of Traditional Chinese Medicine, Changchun 130117, China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Analysis, School of Pharmaceutical Sciences, Changchun University of Traditional Chinese Medicine, Changchun 130117, China.
authors.forename: Zhi-Meng
authors.initials: ZM
authors.lastname: Li
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Analysis, School of Pharmaceutical Sciences, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. Electronic address: caiguangzhi@126.com.
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Cai
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Analysis, School of Pharmaceutical Sciences, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. Electronic address: gjy0431@126.com.
authors.forename: Ji-Yu
authors.initials: JY
authors.lastname: Gong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D012503:Saponins; C584473:raddeanin A; D002945:Cisplatin
keywords: Apoptosis; Cisplatin; Drug combination; Human hepatocellular carcinoma; Raddeanin A