title: pncA Gene Mutations Associated with Pyrazinamide Resistance in Drug-Resistant Tuberculosis, South Africa and Georgia.
abstract: Although pyrazinamide is commonly used for tuberculosis treatment, drug-susceptibility testing is not routinely available. We found polymorphisms in the pncA gene for 70% of multidrug-resistant and 96% of extensively drug-resistant Mycobacterium tuberculosis isolates from South Africa and Georgia. Assessment of pyrazinamide susceptibility may be prudent before using it in regimens for drug-resistant tuberculosis.
authors.forename: Salim
authors.initials: S
authors.lastname: Allana
authors.forename: Elena
authors.initials: E
authors.lastname: Shashkina
authors.forename: Barun
authors.initials: B
authors.lastname: Mathema
authors.forename: Nino
authors.initials: N
authors.lastname: Bablishvili
authors.forename: Nestani
authors.initials: N
authors.lastname: Tukvadze
authors.forename: N Sarita
authors.initials: NS
authors.lastname: Shah
authors.forename: Russell R
authors.initials: RR
authors.lastname: Kempker
authors.forename: Henry M
authors.initials: HM
authors.lastname: Blumberg
authors.forename: Pravi
authors.initials: P
authors.lastname: Moodley
authors.forename: Koleka
authors.initials: K
authors.lastname: Mlisana
authors.forename: James C M
authors.initials: JC
authors.lastname: Brust
authors.forename: Neel R
authors.initials: NR
authors.lastname: Gandhi
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D000995:Antitubercular Agents / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D054908:Extensively Drug-Resistant Tuberculosis / Q000453:epidemiology* / Q000382:microbiology; D005846:Georgia (Republic) / Q000453:epidemiology; D006801:Humans; D009154:Mutation; D011718:Pyrazinamide / Q000494:pharmacology*; D013019:South Africa / Q000453:epidemiology
chemical_list: D000995:Antitubercular Agents; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis
keywords: Georgia; South Africa; bacteria; drug resistance; pyrazinamide; sequencing; tuberculosis; tuberculosis and other mycobacteria