title: Screening of filamentous fungi to produce xylanase and xylooligosaccharides in submerged and solid-state cultivations on rice husk, soybean hull, and spent malt as substrates.
abstract: We investigated the enzymatic complex produced by selected fungi strains isolated from the environment using the agro-industrial residues rice husk, soybean hull, and spent malt as substrates. Microbial growth was carried out in solid-state cultivation (SSC) and in submerged cultivations (SC) and the enzymatic activities of xylanase, cellulase, β-xylosidase, and β-glucosidase were determined. All substrates were effective in inducing enzymatic activities, with one strain of Aspergillus brasiliensis BLf1 showing maximum activities for all enzymes, except for cellulases. Using this fungus, the enzymatic activities of xylanase, cellulase, and β-glucosidase were generally higher in SSC compared to SC, producing maxima activities of 120.5, 25.3 and 47.4 U g-1of dry substrate, respectively. β-xylosidase activity of 28.1 U g-1of dry substrate was highest in SC. Experimental design was carried out to optimize xylanase activity by A. brasiliensis BLf1 in SSC using rice husk as substrate, producing maximum xylanase activity 183.5 U g-1dry substrate, and xylooligosaccharides were produced and characterized. These results suggest A. brasiliensis BLf1 can be used to produce important lytic enzymes to be applied in the preparation of xylooligosaccharides.
authors.affiliation: Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: da Silva Menezes
authors.affiliation: Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Daniele Misturini
authors.initials: DM
authors.lastname: Rossi
authors.affiliation: Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. mazayub@ufrgs.br.
authors.forename: Marco Antônio Záchia
authors.initials: MA
authors.lastname: Ayub
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D061249:Batch Cell Culture Techniques; D002480:Cellulase / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology / Q000254:growth & development* / Q000302:isolation & purification; D005965:Glucuronates / Q000096:biosynthesis*; D009844:Oligosaccharides / Q000096:biosynthesis*; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D013025:Glycine max / Q000382:microbiology*; D013379:Substrate Specificity; D014995:Xylosidases / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D005965:Glucuronates; D009844:Oligosaccharides; C570991:xylooligosaccharide; D014995:Xylosidases; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Agro-residues; Aspergillus brasiliensis BLf1; Solid-state cultivation; Submerged cultivations; Xylanase; Xylooligosaccharides