title: Isolation and Characterisation of a Molybdenum-reducing and Metanil Yellow Dye-decolourisingBacillussp. strain Neni-10 in Soils from West Sumatera, Indonesia.
abstract: A molybdenum reducing bacterium with the novel ability to decolorise the azo dye Metanil Yellow is reported. Optimal conditions for molybdenum reduction were pH 6.3 and at 34°C. Glucose was the best electron donor. Another requirement includes a narrow phosphate concentration between 2.5 and 7.5 mM. A time profile of Mo-blue production shows a lag period of approximately 12 hours, a maximum amount of Mo-blue produced at a molybdate concentration of 20 mM, and a peak production at 52 h of incubation. The heavy metals mercury, silver, copper and chromium inhibited reduction by 91.9, 82.7, 45.5 and 17.4%, respectively. A complete decolourisation of the dye Metanil Yellow at 100 and 150 mg/L occurred at day three and day six of incubations, respectively. Higher concentrations show partial degradation, with an approximately 20% decolourisation observed at 400 mg/L. The bacterium is partially identified based on biochemical analysis asBacillussp. strain Neni-10. The absorption spectrum of the Mo-blue suggested the compound is a reduced phosphomolybdate. The isolation of this bacterium, which shows heavy metal reduction and dye-decolorising ability, is sought after, particularly for bioremediation.
authors.affiliation: Department of Agricultural Engineering, Faculty of Agricultural Technology, Andalas University, Padang 25163, Indonesia.
authors.forename: Rusnam
authors.initials: R
authors.lastname: Mansur
authors.affiliation: Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Padang 25163, Indonesia; Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Neni
authors.initials: N
authors.lastname: Gusmanizar
authors.affiliation: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Muhamad Akhmal Hakim
authors.initials: MA
authors.lastname: Roslan
authors.affiliation: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Siti Aqlima
authors.initials: SA
authors.lastname: Ahmad
authors.affiliation: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Mohd Yunus
authors.initials: MY
authors.lastname: Shukor
keywords: Azo Dye; Bacillus sp.; Metanil Yellow; Molybdenum Blue; Molybdenum-reducing