title: PAX3 inhibits β-Tubulin-III expression and neuronal differentiation of neural stem cell.
abstract: PAX3 functions at the nodal point in neural stem cell maintenance and differentiation. Using bioinformatics methods, we identified PAX3 as a potential regulator of β-Tubulin-III (TUBB3) gene transcription, and the results indicated that PAX3 might be involved in neural stem cell (NSC) differentiation by orchestrating the expression of cytoskeletal proteins. In the present study, we reported that PAX3 could inhibit the differentiation of NSCs and the expression of TUBB3. Further, using luciferase and electrophoretic mobility shift assays, we demonstrated that PAX3 could bind to the promoter region of TUBB3 and inhibit TUBB3 transcription. Finally, we confirmed that PAX3 could bind to the promoter region of endogenous TUBB3 in the native chromatin of NSCs. These findings indicated that PAX3 is a pivotal factor targeting various molecules during differentiation of NSCs in vitro.
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Sixian
authors.initials: S
authors.lastname: Cao
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Du
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Hengrong
authors.initials: H
authors.lastname: Lin
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Zuming
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong, Jiangsu Province 226001, PR China. Electronic address: liuyan@ntu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D000072041:PAX3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D017207:Rats, Sprague-Dawley; D015533:Transcriptional Activation; D014404:Tubulin / Q000235:genetics*
chemical_list: D000072041:PAX3 Transcription Factor; C530820:PAX3 protein, rat; D034741:RNA, Small Interfering; C488416:Tubb3 protein, rat; D014404:Tubulin
keywords: Neural stem cells; Neuronal differentiation; Paired box 3 protein; β-Tubulin-III