title: Hydrogen peroxide as a new defensive compound in "benzoyl cyanide" producing polydesmid millipedes.
abstract: Hydrogen peroxide was newly and simultaneously demonstrated with well-known hydrogen cyanide as a component of defensive secretions of "benzoyl cyanide" producing polydesmid millipedes. Presence of hydrogen peroxide was successively evidenced by Trinder reagent's spray with colorless as well as oily smears of defensive secretions containing benzoyl cyanide and hydrogen cyanide by alkaline picrate paper treatment. Linear correlation was demonstrated between quantities of hydrogen peroxide and benzoyl cyanide. By qualitative assay, seven benzoyl cyanide containing polydesmidans (six species of adults and one species of a nymph at stadium I) tested positive to Trinder reagent, indicative of the presence of hydrogen peroxide (together with hydrogen cyanide), while two cyanogenic species without benzoyl cyanide exhibited negative responses to the reagent. Two types of millipedes were elucidated as species of cyanogenic Polydesmida.
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.|Asano Active Enzyme Molecule Project, JST, ERATO, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kuwahara
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.|Asano Active Enzyme Molecule Project, JST, ERATO, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.|Asano Active Enzyme Molecule Project, JST, ERATO, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Ichiki
authors.affiliation: Faculty of Education, Kumamoto University, Kumamoto, 860-8555, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanabe
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan. asano@pu-toyama.ac.jp.|Asano Active Enzyme Molecule Project, JST, ERATO, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan. asano@pu-toyama.ac.jp.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Asano
mesh_terms: D000818:Animals; D001181:Arthropods / Q000737:chemistry* / Q000502:physiology; D003486:Cyanides / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis*; D009758:Nymph; D013045:Species Specificity
chemical_list: D003486:Cyanides; D006861:Hydrogen Peroxide
keywords: Benzoyl cyanide; Defensive secretion; Hydrogen peroxide; Mandelonitrile oxidase; Millipede; Polydesmida; Trinder reagent