title: Differentiation and Metabolic Interrogation of Human Adipocytes.
abstract: Adipocytes differentiated from preadipocytes provide a valuable model for the study of human adipocyte metabolism. We describe methods for isolation of human stromal vascular cells, expansion of preadipocytes, differentiation into mature adipocytes, and in vitro metabolic interrogation of adipocytes.
authors.affiliation: Section of General Surgery, Department of Surgery, University of Michigan Medical School, 2210 Taubman Center-5343, 1500 E. Medical Center Drive, Ann Arbor, MI, USA.
authors.forename: Nicki A
authors.initials: NA
authors.lastname: Baker
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Lindsey A
authors.initials: LA
authors.lastname: Muir
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Carey N
authors.initials: CN
authors.lastname: Lumeng
authors.affiliation: Section of General Surgery, Department of Surgery, University of Michigan Medical School, 2210 Taubman Center-5343, 1500 E. Medical Center Drive, Ann Arbor, MI, USA. rorourke@umich.edu.|Department of Surgery, Ann Arbor Veteran's Administration Hospital, Ann Arbor, MI, USA. rorourke@umich.edu.
authors.forename: Robert W
authors.initials: RW
authors.lastname: O'Rourke
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000943:Antigens, Differentiation; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D004734:Energy Metabolism; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D050356:Lipid Metabolism
chemical_list: D000943:Antigens, Differentiation; D005947:Glucose
keywords: Adipocyte; Adipose tissue; Differentiation; Glucose uptake; Lipogenesis; Lipolysis; Preadipocyte; Stromal vascular cell fraction