title: A Novel Dendrimeric "Glue" for Adhesion of Phosphatidyl Choline-Based Liposomes.
abstract: The interaction of phosphatidyl choline-cholesterol liposomes incorporating dihexadecyl phosphate as recognizable lipid with complementary guanidinylated diaminobutane poly(propylene imine) dendrimers of the fourth and fifth generation afforded liposome-dendrimer aggregates which were redispersed by the addition of an excess of a phosphate buffer. The higher generation dendrimeric derivative proved more effective when interacted with liposomes. This behavior was attributed to multivalent effects, which, as generally established, enhance the reactivity of multifunctional particles. Turbidimetry, atomic force microscopy (AFM) and optical microscopy were used for investigating the interaction of the complementary particles while the redispersion of the aggregates was studied by transmission electron microscopy (TEM). Liposomal membrane stability in the collapse and redispersion processes was assessed by the calcein fluorescence method, TEM, and AFM.
authors.affiliation: Institute of Physical Chemistry, NCSR "Demokritos", 15310 Aghia Paraskevi, Attiki, Greece, and Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
authors.forename: Zili
authors.initials: Z
authors.lastname: Sideratou
authors.affiliation: Institute of Physical Chemistry, NCSR "Demokritos", 15310 Aghia Paraskevi, Attiki, Greece, and Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
authors.forename: John
authors.initials: J
authors.lastname: Foundis
authors.affiliation: Institute of Physical Chemistry, NCSR "Demokritos", 15310 Aghia Paraskevi, Attiki, Greece, and Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Tsiourvas
authors.affiliation: Institute of Physical Chemistry, NCSR "Demokritos", 15310 Aghia Paraskevi, Attiki, Greece, and Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
authors.forename: Ioannis P
authors.initials: IP
authors.lastname: Nezis
authors.affiliation: Institute of Physical Chemistry, NCSR "Demokritos", 15310 Aghia Paraskevi, Attiki, Greece, and Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Papadimas
authors.affiliation: Institute of Physical Chemistry, NCSR "Demokritos", 15310 Aghia Paraskevi, Attiki, Greece, and Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
authors.forename: Constantinos M
authors.initials: CM
authors.lastname: Paleos