title: Lycium barbarum L. Polysaccharide (LBP) Reduces Glucose Uptake via Down-Regulation of SGLT-1 in Caco2 Cell.
abstract: Lycium barbarumL. polysaccharide (LBP) is prepared fromLycium barbarumL. (L. barbarum), which is a traditional Chinese medicine. LPB has been shown to have hypoglycemic effects. In order to gain some mechanistic insights on the hypoglycemic effects of LBP, we investigated the uptake of LBP and its effect on glucose absorption in the human intestinal epithelial cell line Caco2 cell. The uptake of LBP through Caco2 cell monolayer was time-dependent and was inhibited by phloridzin, a competitive inhibitor of SGLT-1. LPB decreased the absorption of glucose in Caco2 cell, and down-regulated the expression of SGLT-1. These results suggest that LBP might be transported across the human intestinal epithelium through SGLT-1 and it inhibits glucose uptake via down-regulating SGLT-1.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. 18169010183@163.com.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Sanitary Chemistry, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. yangxh@nxmu.edu.cn.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Experimental Center, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. caiqian1212@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Experimental Center, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. renbb@nxmu.edu.cn.
authors.forename: Binbin
authors.initials: B
authors.lastname: Ren
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. yanzide80@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Qiu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. CHZ.Liu08@gmail.com.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Yao
mesh_terms: D018938:Caco-2 Cells; D050260:Carbohydrate Metabolism; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D051273:Glucose Transporter Type 2 / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007408:Intestinal Absorption; D032305:Lycium / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D051273:Glucose Transporter Type 2; D007004:Hypoglycemic Agents; D012333:RNA, Messenger; C495680:SLC2A2 protein, human; C495674:SLC5A1 protein, human; D051269:Sodium-Glucose Transporter 1; C500223:lycium barbarum polysaccharide; D005947:Glucose
keywords: Caco2 cell monolayer; GLUT-2; Lycium barbarum L. polysaccharide; SGLT-1