title: Development of an UPLC-MS/MS micromethod for quantitation of cinitapride in plasma and its application in a pharmacokinetic interaction trial.
abstract: OBJECTIVE
  Cinitapride (CIN) is a benzamide-derived molecule used for the treatment of gastroesophageal reflux and dyspepsia. Its pharmacokinetics are controversial due to the use of supratherapeutic doses and the lack of sensitive methodology. Therefore, a sensitive and accurate micromethod was developed for its quantitation in human plasma.
  
  
  RESULTS
  CIN was extracted from 300 µl of heparinized plasma by liquid-liquid extraction using cisapride as internal standard, and analyzed with an ultra performance liquid chromatograph employing positive multiple-reaction monitoring-MS.
  
  
  CONCLUSIONS
  The method proved to be rapid, accurate and stable within a range between 50 and 2000 pg/ml and was successfully validated and applied in a pharmacokinetic interaction trial, where it was demonstrated that oral co-administration of simethicone does not modify the bioavailability of CIN.
authors.affiliation: Global Bioanalytical Consulting, S.C., Heriberto Frías No. 1439, Int. 402, Col. Del Valle, Del. Benito Juárez, Mexico City 03100, Mexico.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Marcelín-Jiménez
authors.affiliation: Analytical & Statistics Unit, Pharmometrica (Pharmet, S.A de C.V.), Av. Eje 5 Norte 990, Edificio 'C', Planta Baja, Mexico City 02230, Mexico.
authors.forename: Leticia
authors.initials: L
authors.lastname: Contreras
authors.affiliation: Analytical & Statistics Unit, Pharmometrica (Pharmet, S.A de C.V.), Av. Eje 5 Norte 990, Edificio 'C', Planta Baja, Mexico City 02230, Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Esquivel
authors.affiliation: Pharmometrica Clinical Facility, Av. de las Granjas 972, LC 08A, Mexico City 02230, Mexico.
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Ávila
authors.affiliation: Analytical & Statistics Unit, Pharmometrica (Pharmet, S.A de C.V.), Av. Eje 5 Norte 990, Edificio 'C', Planta Baja, Mexico City 02230, Mexico.
authors.forename: Dany
authors.initials: D
authors.lastname: Batista
authors.affiliation: Global Bioanalytical Consulting, S.C., Heriberto Frías No. 1439, Int. 402, Col. Del Valle, Del. Benito Juárez, Mexico City 03100, Mexico.
authors.forename: Alionka P
authors.initials: AP
authors.lastname: Ángeles
authors.affiliation: Global Bioanalytical Consulting, S.C., Heriberto Frías No. 1439, Int. 402, Col. Del Valle, Del. Benito Juárez, Mexico City 03100, Mexico.
authors.forename: Alberto
authors.initials: A
authors.lastname: García-González
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D019540:Area Under Curve; D001549:Benzamides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D018592:Cross-Over Studies; D064368:Healthy Volunteers; D006801:Humans; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D008875:Middle Aged; D012015:Reference Standards; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D055815:Young Adult
chemical_list: D001549:Benzamides; C045316:cinitapride
keywords: HPLC–MS; cinitapride; gastroesophageal reflux; simethicone