title: Capillary electrophoresis in the context of drug discovery.
abstract: Capillary Electrophoresis is a very efficient and resolutive separation technique used for many years in the analytical field. Despite all its assets, CE remains poorly used in drug discovery. This can be explained by the relatively low number of experienced CE practitioners, the maturity of HPLC in the pharmaceutical industry and some intrinsic limitations of the technique. The objective of this review is to focus our attention on recent developments of this technique in three different drug discovery areas: bioassays, drug-plasma interactions and drug metabolism studies. These developments were based on two important abilities of CE: the capacity to measure non-covalent interactions in solution and the ability to use a portion of the capillary as a reactor while the rest of the capillary is used for the separation of the product of the reaction.
authors.affiliation: Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium.
authors.forename: Elena
authors.initials: E
authors.lastname: Farcaş
authors.affiliation: Namur Medicine & Drug Innovation Center (NaMEDIC - Narilis), University of Namur, Belgium.
authors.forename: Lionel
authors.initials: L
authors.lastname: Pochet
authors.affiliation: Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.affiliation: Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium.
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Servais
authors.affiliation: Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium. Electronic address: marianne.fillet@ulg.ac.be.
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
mesh_terms: D002851:Chromatography, High Pressure Liquid; D055808:Drug Discovery; D004345:Drug Industry; D019075:Electrophoresis, Capillary
keywords: Activity assays; Affinity assays; Bioassays; Capillary electrophoresis; Drug metabolism