title: Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake.
abstract: The mixed-valent oxo-bridged ruthenium complex [(HCO2)(NH3)4Ru(μ-O)Ru(NH3)4(O2CH)]3+, known as Ru360, is a selective inhibitor of mitochondrial calcium uptake. Although this compound is useful for studying the role of mitochondrial calcium in biological processes, its widespread availability is limited because of challenges in purification and characterization. Here, we describe our investigations of three different synthetic methods for the preparation of a functional analogue of this valuable compound. We demonstrate that this analogue, isolated from our procedures, exhibits potent mitochondrial calcium uptake inhibitory properties in permeabilized HeLa cells and in isolated mitochondria.
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University , Ithaca, New York 14853, United States.
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Nathan
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University , Ithaca, New York 14853, United States.
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Pino
authors.affiliation: Gene Center, Department of Biochemistry, Ludwig-Maximilans Universität München , Munich, Germany.|Institute of Human Genetics, Helmholtz Zentrum München , Neuherberg, Germany.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Arduino
authors.affiliation: Gene Center, Department of Biochemistry, Ludwig-Maximilans Universität München , Munich, Germany.|Institute of Human Genetics, Helmholtz Zentrum München , Neuherberg, Germany.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Perocchi
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University , Ithaca, New York 14853, United States.
authors.forename: Samantha N
authors.initials: SN
authors.lastname: MacMillan
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University , Ithaca, New York 14853, United States.
authors.forename: Justin J
authors.identifier: http://orcid.org/0000-0002-4086-7982
authors.initials: JJ
authors.lastname: Wilson
mesh_terms: D002118:Calcium / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects*; D017975:Ruthenium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C112020:Ru 360; D017975:Ruthenium Compounds; D002118:Calcium