title: MicroRNA-520e suppresses non-small-cell lung cancer cell growth by targeting Zbtb7a-mediated Wnt signaling pathway.
abstract: MicroRNAs (miRNAs) that negatively regulated gene expression have emerged as novel therapeutic target for non-small-cell lung cancer (NSCLC) treatment. In this study, we investigated the potential role of miR-520e and examined its functional role in NSCLCs. Loss-and-gain of function assays show that miR-520e significantly modulated NSCLC cell growth, cell invasion and cell migration via directly targeting the 3'-untranslated region (UTR) of Zbtb7a and therefore reduced Zbtb7a protein level. These effects of miR-520e on NSCLC progression could be rescued by Zbtb7a overexpression in A549 cells. Furthermore, both of miR-520e level and Zbtb7a level were correlated with Wnt signaling in NSCLC cells. Taken together, these results indicate that overexpressing miR-520e is involved in regulating the NSCLC cell growth, invasion and migration by targeting Zbtb7a partly depending on Wnt signaling.
authors.affiliation: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zhijun
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China. Electronic address: btgyia@163.com.
authors.forename: He
authors.initials: H
authors.lastname: Jingkang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000072283:A549 Cells; D001483:Base Sequence; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; C575048:MIRN520 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors; C485514:ZBTB7A protein, human
keywords: MicroRNAs; Non-small-cell lung cancer; Wnt signaling; Zbtb7a; miR-520e