title: The effects of endophytic bacterium SaMR12 on Sedum alfredii Hance metal ion uptake and the expression of three transporter family genes after cadmium exposure.
abstract: A hydroponic experiment was conducted to investigate the effects of an endophytic bacterium SaMR12 on Sedum alfredii Hance metal ion accumulation, chlorophyll concentration, and the expression of three metal transporter families, zinc-regulated transporters, iron-regulated transporter-like protein (ZIP); natural resistance-associated macrophage protein; and heavy metal ATPase (HMA) at different Cd treatment levels. The results showed that at relatively low Cd conditions (≤25 μM), SaMR12 demonstrated a 19.5-27.5% increase in Fe, a 46.7-90.7% increase in Zn, and a 7.9-43.7% increase in Cu content in the shoot and elevated expression of SaIRT1, SaZIP3, SaHMA2, and SaNramp3 in the shoot and SaZIP1, SaHMA2, SaNramp1, and SaNramp3 in the root. At high Cd conditions (100 and 400 μM), SaMR12 demonstrated a 16.4-18.5% increase in leaf chlorophyll concentration, a 18.9-23.2% increase in Fe, and a 15.4-17.5% increase in Mg content in the shoot and elevated expression of SaZIP3, SaNramp6, SaHMA2, and SaHMA3 in the shoot and SaZIP3, SaNarmp1, SaNarmp3, and SaNarmp6 in the root. These results indicated that SaMR12 can elevate essential metal ion uptake and regulate the expression of transport genes to promote plant growth and enhance Cd tolerance and uptake to improve Cd accumulation up to 118-130%.
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Fengshan
authors.initials: F
authors.lastname: Pan
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Luo
authors.affiliation: Agricultural Technology Extension Center of Shaoxing, Shaoxing, 312099, Zhejiang Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Ye
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Meng
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wu
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Bao
authors.initials: B
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Xuerui
authors.initials: X
authors.lastname: Cao
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China. yfeng@zju.edu.cn.
authors.forename: Ying
authors.identifier: http://orcid.org/0000-0001-7846-3855
authors.initials: Y
authors.lastname: Feng
mesh_terms: D001673:Biodegradation, Environmental; D002104:Cadmium; D027682:Cation Transport Proteins; D018517:Plant Roots / Q000378:metabolism; D031963:Sedum / Q000378:metabolism*; D015032:Zinc; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D002104:Cadmium; D027682:Cation Transport Proteins; D015032:Zinc; D000099173:Natural Resistance-associated Macrophage Protein 1
keywords: Cd; Chlorophyll; Fe; HMA; Hyperaccumulator; NRAMP; Plant growth promoting bacteria; ZIP