title: Isolation ofClostridium difficileand molecular detection of binary and A/B toxins in faeces of dogs.
abstract: The aim of this study was to isolateClostridium difficilefrom dogs' faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs' faeces were collected. The isolation ofC. difficilewas performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification oftcdA,tcdB,cdtAandcdtBgenes and single method was carried out for detection oftcdC. Twelve samples (7.9%) were positive in bacteriological assay and based on molecular assay, 66.7% of the isolates (8 of 12C. difficileisolated) had shown tcdA+, tcdB+profile. This is the first investigation on molecular assay ofC. difficilein Iran's dog population.
authors.affiliation: Ph.D. Student in Bacteriology, Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ghavidel
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Salari Sedigh
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: J
authors.initials: J
authors.lastname: Razmyar
keywords: Clostridium difficile; Dog; Molecular detection