title: Detection and quantification of 12 anabolic steroids and analogs in human whole blood and 20 in hair using LC-HRMS/MS: application to real cases.
abstract: We developed and validated a method to detect and quantify 12 anabolic steroids in blood (androstenedione, dihydrotestosterone, boldenone, epitestosterone, mesterolone, methandienone, nandrolone, stanozolol, norandrostenedione, tamoxifene, testosterone, trenbolone) and eight more in hair samples (nandrolone phenylpropionate, nandrolone decanoate, testosterone propionate, testosterone benzoate, testosterone cypionate, testosterone decanoate, testosterone phenylpropionate, testosterone undecanoate) using liquid chromatography coupled to high-resolution mass spectrometry. This method used a benchtop Orbitrap mass spectrometer operating with an APCI probe under positive ionization mode. Analysis was realized in full scan experiment with a nominal resolving power of 140,000. After addition of the internal standard (testosterone-D3) and incubation in phosphate buffer pH = 5 for hair, 200 μL of blood and 30 mg of hair samples were extracted with heptane. LOQ and LOD were determined at 5 and 1 ng mL-1in whole blood and 10 to 100 pg mg-1and 2 to 20 pg mg-1in hair according to the compounds, respectively. The method was linear in the 5-1000 ng mL-1range in whole blood and between 10 or 100 pg mg-1and 1000 pg mg-1in hair with correlation coefficients >0.99, and intra- and inter-day accuracy and precision were <14.8% for all compounds except for some esters in hairs (<19.9%) probably due to an important matrix effect for these compounds. This sensitive and specific method to detect anabolic steroids has been successfully applied to two real cases, for which various anabolic steroids in whole blood, urine, and hair were identified and quantified.
authors.affiliation: MassSpecLab, Plateforme de Spectrométrie de Masse, UFR des Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, 2 Avenue de la Source de la Bièvre, 78180, Montigny le Bretonneux, France.|Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, AP-HP, 104 Boulevard R. Poincaré, 92380, Garches, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fabresse
authors.affiliation: MassSpecLab, Plateforme de Spectrométrie de Masse, UFR des Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, 2 Avenue de la Source de la Bièvre, 78180, Montigny le Bretonneux, France.|Laboratoire de Pharmacologie UPRES EA220, Hôpital Foch, 92150, Suresnes, France.
authors.forename: Stanislas
authors.initials: S
authors.lastname: Grassin-Delyle
authors.affiliation: MassSpecLab, Plateforme de Spectrométrie de Masse, UFR des Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, 2 Avenue de la Source de la Bièvre, 78180, Montigny le Bretonneux, France.|Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, AP-HP, 104 Boulevard R. Poincaré, 92380, Garches, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Etting
authors.affiliation: MassSpecLab, Plateforme de Spectrométrie de Masse, UFR des Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, 2 Avenue de la Source de la Bièvre, 78180, Montigny le Bretonneux, France. jean-claude.alvarez@aphp.fr.|Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, AP-HP, 104 Boulevard R. Poincaré, 92380, Garches, France. jean-claude.alvarez@aphp.fr.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Alvarez
mesh_terms: D000328:Adult; D045930:Anabolic Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D053593:Forensic Toxicology; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D015813:Substance Abuse Detection / Q000379:methods*; D055815:Young Adult
chemical_list: D045930:Anabolic Agents
keywords: Anabolic steroids; Forensic toxicology; Hair analysis; High-resolution mass spectrometry; Orbitrap