title: Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells.
abstract: Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-E1 cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yasukawa
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Tanabe
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Tsuda
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Oral Health Sciences, Nihon University School of Dentistry.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kawato
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry.
authors.forename: Naoto
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Department of Oral Health Sciences, Nihon University School of Dentistry.
authors.forename: Masao
authors.initials: M
authors.lastname: Maeno
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry.
authors.forename: Bunnai
authors.initials: B
authors.lastname: Ogiso
mesh_terms: D017607:Aluminum Compounds; D000818:Animals; D017610:Calcium Compounds; D002454:Cell Differentiation; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D051379:Mice; D010012:Osteogenesis; D010087:Oxides; D060888:Real-Time Polymerase Chain Reaction; D044169:Receptors, Calcium-Sensing; D017640:Silicates
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D044169:Receptors, Calcium-Sensing; D017640:Silicates; C086631:mineral trioxide aggregate
keywords: Calcium-sensing receptor; Mineral trioxide aggregate; Osteogenesis