title: Antiviral activity of phosphorylated Radix Cyathulae officinalis polysaccharide against Canine Parvovirus in vitro.
abstract: Phosphorylated Radix Cyathulae officinalis Kuan polysaccharides (pRCPS) was prepared according to three-factors, ratio of STMP (%) and STPP (%), reaction time and reaction temperature, and three level L9(34) orthogonal design. The antiviral activity of nine pRCPS (pRCPS1-9) was systematically evaluated by three methods pre-adding mode, mixed mode, and post-adding mode. Cellular activity was tested by the CCK-8 assay. The results showed that the optimal modification conditions were the ratio of STMP (%) and STPP (%) 1:4, reaction time 2h and reaction temperature 65°C. Six pRCPS (pRCPS1-4, pRCPS7, pRCPS9) exhibited significant anti-viral activity in pre-adding mode (P<0.05). Eight pRCPS (pRCPS1-4, pRCPS5, pRCPS6, pRCPS7, and pRCPS9)showed dramatic anti-viral activity in the mixed mode (P<0.05). Six pRCPS (pRCPS1-4, pRCPS6, pRCPS9) showed antiviral activity in the post-adding mode (P<0.05). Taken together, four pRCPS (pRCPS1-4) demonstrated significant antiviral activity in all the test modes (P<0.05) and their antiviral efficacy were significantly stronger than unmodified RCPS (P<0.05). Those results indicated that four pRCPS (pRCPS1-4) possessed significant antiviral activity and may have potential as a new CPV therapeutic compound, and phosphorylation could significantly enhance the antiviral effect of RCPS. Moreover, phosphorylation modification technique could be valuable as a method to promote the antiviral activity of polysaccharide.
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China. Electronic address: fenghaiborc@126.com.
authors.forename: Haibo
authors.initials: H
authors.lastname: Feng
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, 610051, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China; Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, 610051, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yi
mesh_terms: D000998:Antiviral Agents / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D019684:Magnoliopsida / Q000737:chemistry*; D017993:Parvovirus, Canine / Q000187:drug effects* / Q000502:physiology; D010766:Phosphorylation; D011134:Polysaccharides / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D012449:Safety
chemical_list: D000998:Antiviral Agents; D011134:Polysaccharides
keywords: Antiviral activity; Phosphorylation modification; Radix Cyathula officinalis Kuan Polysaccharide