title: -Genomic data mining of the marine actinobacteriaStreptomycessp. H-KF8 unveils insights into multi-stress related genes and metabolic pathways involved in antimicrobial synthesis.
abstract: Streptomycessp. H-KF8 is an actinobacterial strain isolated from marine sediments of a Chilean Patagonian fjord. Morphological characterization together with antibacterial activity was assessed in various culture media, revealing a carbon-source dependent activity mainly against Gram-positive bacteria (S. aureusandL. monocytogenes). Genome mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic gene clusters (BGCs) for secondary metabolites, where among them, 81% have low similarities with known BGCs. In addition, a genomic search inStreptomycessp. H-KF8 unveiled the presence of a wide variety of genetic determinants related to heavy metal resistance (49 genes), oxidative stress (69 genes) and antibiotic resistance (97 genes). This study revealed that the marine-derivedStreptomycessp. H-KF8 bacterium has the capability to tolerate a diverse set of heavy metals such as copper, cobalt, mercury, chromate and nickel; as well as the highly toxic tellurite, a feature first time described forStreptomyces. In addition,Streptomycessp. H-KF8 possesses a major resistance towards oxidative stress, in comparison to the soil reference strainStreptomyces violaceoruberA3(2). Moreover,Streptomycessp. H-KF8 showed resistance to 88% of the antibiotics tested, indicating overall, a strong response to several abiotic stressors. The combination of these biological traits confirms the metabolic versatility ofStreptomycessp. H-KF8, a genetically well-prepared microorganism with the ability to confront the dynamics of the fjord-unique marine environment.
authors.affiliation: Departmento de Química & Centro de Biotecnología, Universidad Técnica Federico Santa María , Valparaiso , Chile.
authors.forename: Agustina
authors.initials: A
authors.lastname: Undabarrena
authors.affiliation: Centro de Genética y Genómica, Facultad de Medicina Clinica Alemana, Universidad del Desarrollo , Santiago , Chile.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Ugalde
authors.affiliation: Departmento de Química & Centro de Biotecnología, Universidad Técnica Federico Santa María , Valparaiso , Chile.
authors.forename: Michael
authors.initials: M
authors.lastname: Seeger
authors.affiliation: Departmento de Química & Centro de Biotecnología, Universidad Técnica Federico Santa María , Valparaiso , Chile.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Cámara
keywords: Abiotic stressors; Antimicrobial activity; Biosynthethic gene clusters; Chilean Patagonian fjord; Genome mining; Heavy metal tolerance; Marine actinomycete; Streptomyces