title: Endometrial mRNA expression of prostaglandin synthase enzymes PTGS 2, PTGFS and mPTGES 1 in repeat-breeding cows with cytologically determined endometritis.
abstract: Little is known about the inflammatory response of the endometrium in repeat-breeding cows with subclinical endometritis (SE). The objective of this study was to evaluate the mRNA expression of prostaglandin-endoperoxide synthase 2 (PTGS 2), prostaglandin F2αsynthase (PTGFS) and prostaglandin E2 microsomal synthase 1 (mPTGES 1) in the endometrium of repeat-breeding cows with and without SE. SE was diagnosed cytologically using the cytobrush method, with the threshold being set at 5% polymorphonuclear neutrophils. Biopsy samples were obtained from the endometrium of repeat-breeding cows with SE (n = 10) and without SE (n = 10). The mRNA expression of the synthases was evaluated using qRT-PCR. Significantly higher (P < 0.05) expression of the PTGS 2 gene was detected in the repeat breeders with SE, whereas there was no significant difference in the expression of PTGFS and mPTGES 1 mRNAs between repeatbreeding cows with SE and those without it (P > 0.05). Our study confirms that increased endometrial expression of the PTGS 2 gene is involved in the inflammatory response in repeat breeders.
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury , Oczapowskiego 14, 10-719 Olsztyn , Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Janowski
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury , Oczapowskiego 14, 10-719 Olsztyn , Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Barański
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Science , Olsztyn , Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Łukasik
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Science , Olsztyn , Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Skarżyński
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury , Oczapowskiego 14, 10-719 Olsztyn , Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Zduńczyk
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury , Oczapowskiego 14, 10-719 Olsztyn , Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Malinowska
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D004716:Endometritis / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*; D004717:Endometrium / Q000201:enzymology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000071876:Prostaglandin-E Synthases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D051546:Cyclooxygenase 2; D000071876:Prostaglandin-E Synthases
keywords: Cows; PTGFS; PTGS 2; mPTGES 1; repeat breeding