title: Long non-coding RNA MIAT acts as a biomarker in diabetic retinopathy by absorbingmiR-29band regulating cell apoptosis.
abstract: Diabetic retinopathy (DR) is a complication of diabetes mellitus (DM) and is the leading cause of vision loss globally. However, the pathogenic mechanism and clinical therapy still needs further improvement. The biologic significance of myocardial infarction associated transcript (MIAT) in DR remains unknown. Here, we aim to explore the mechanism between MIAT and DR, which is essential for RD. Streptozotocin (STZ) was used to induce DM mice and high glucose was used to stimulate cells. ChIP was used to detect the binding activity between nuclear factor κB (NF-κB) and the promoter of the MIAT gene, luciferase activity assay was used to detect the target-specific selectivity betweenmiR-29band MIAT. The expressions of MIAT and p-p65 were increased in STZ-induced DM mice and high glucose stimulated rat retinal Müller cells (rMC-1) cells. ChIP results revealed that high glucose promoted the binding activity between NF-κB and MIAT, while Bay11-7082 acted as an inhibitor for NF-κB that suppressed the binding activity.miR-29bcontroled MIAT to regulate its expression and MIAT overexpression suppressedmiR-29b, but promoted Sp1. High glucose stimulation increased the cell apoptosis and decreased the cell activity, while MIAT suppression reversed the effect induced by high glucose, however,miR-29bknockdown reversed the effects induced by MIAT suppression. Our results provided evidence that the mechanism of cell apoptosis in DR might be associated with the regulation of MIAT, however,miR-29bacted as a biomarker that was regulated by MIAT and further regulated cell apoptosis in DR.
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Wenzhou Medical University, Zhejiang Ruian 325200, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Wenzhou Medical University, Zhejiang Ruian 325200, China.
authors.forename: Maochong
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Wenzhou Medical University, Zhejiang Ruian 325200, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Wenzhou Medical University, Zhejiang Ruian 325200, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Wenzhou Medical University, Zhejiang Ruian 325200, China.
authors.forename: Daqiu
authors.initials: D
authors.lastname: Cai
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Wenzhou Medical University, Zhejiang Ruian 325200, China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, The Third Affiliated Hospital of Wenzhou Medical University, Zhejiang Ruian 325200, China chenzhenguo016@163.com.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000473:pathology; D003930:Diabetic Retinopathy / Q000235:genetics* / Q000473:pathology; D005786:Gene Expression Regulation; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D051381:Rats; D015854:Up-Regulation
chemical_list: D005819:Genetic Markers; C547014:MIRN29 microRNA, mouse; C517383:Miat long non-coding RNA; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: MIAT; cell apoptosis; diabetic retinopathy; high glucose; miR-29b