title: Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1.
abstract: BACKGROUND
  Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils.
  
  
  RESULTS
  To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator.
  
  
  CONCLUSIONS
  This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
authors.affiliation: Institut de Bioenginyeria de Catalunya, Parc Científic de Barcelona, 08028, Barcelona, Spain.|Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Avda Diagonal, 643., 08028, Barcelona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Juarez
authors.affiliation: Departamento de Biología Molecular (Universidad de Cantabria) and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC (CSIC-UC), C/Albert Einstein 22, 39011, Santander, Spain.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Villa
authors.affiliation: Departamento de Biología Molecular (Universidad de Cantabria) and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC (CSIC-UC), C/Albert Einstein 22, 39011, Santander, Spain.
authors.forename: Val F
authors.initials: VF
authors.lastname: Lanza
authors.affiliation: Departamento de Biología Molecular (Universidad de Cantabria) and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC (CSIC-UC), C/Albert Einstein 22, 39011, Santander, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Lázaro
authors.affiliation: Departamento de Biología Molecular (Universidad de Cantabria) and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC (CSIC-UC), C/Albert Einstein 22, 39011, Santander, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cruz
authors.affiliation: INBIOP (Instituto de Biociencias de la Patagonia), Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Ruta Provincial No 1, Km 4-Ciudad Universitaria 9000, Comodoro Rivadavia, Chubut, Argentina.
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Alvarez
authors.affiliation: Departamento de Biología Molecular (Universidad de Cantabria) and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC (CSIC-UC), C/Albert Einstein 22, 39011, Santander, Spain. moncalig@unican.es.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Moncalián
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010427:Pentose Phosphate Pathway / Q000235:genetics; D012240:Rhodococcus / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D014280:Triglycerides; D002244:Carbon; D051048:Diacylglycerol O-Acyltransferase; D005947:Glucose
keywords: CRP; Entner-Doudoroff pathway; Nutrient starvation; RNA-Seq; Rhodococcus; Triacylglycerol