title: Selection of Lactic Acid Bacteria with Probiotic Potential Isolated from the Fermentation Process of "Cupuaçu" (Theobroma grandiflorum).
abstract: In the present study, nine lactic acid bacteria isolated from the fermentation process of "cupuaçu" (Theobroma grandiflorum) were selected for probiotic use. In vitro (resistance to gastrointestinal environment, in vitro antagonism and co-aggregation with pathogens) and in vivo (intestinal colonization and ex vivo antagonism in germ-free mice, cumulative mortality, translocation to liver and spleen, histopathological examination of liver and ileum and mRNA cytokine gene expression during an experimental infection with S. Typhimurium) assays were used. Among the nine Lactobacillus strains isolated from the "cupuaçu" fermentation, L. plantarum 81 and L. plantarum 90 were selected as potential probiotics based on better results obtained in in vitro evaluations (production of diffusible inhibitory compounds and co-aggregation) as well as in vivo experiments (resistance to gastrointestinal environment, ex vivo antagonism, higher survival after enteropathogen challenge, lower hepatic translocation of enteropathogen, lower histopathological lesions in ileum and liver and anti-inflammatory pattern of immunological response). Concluding, L. plantarum 81 and L. plantarum 90 showed in vitro and in vivo capacities for probiotic use through different mechanisms of protection and its origin would allow an easier adaptation in an alimentary matrix for its administration.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brazil.
authors.forename: Roberta Maria Santos
authors.initials: RMS
authors.lastname: Ornellas
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brazil.
authors.forename: Tiza Teles
authors.initials: TT
authors.lastname: Santos
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Leonardo Borges
authors.initials: LB
authors.lastname: Arcucio
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Sávio Henrique Cicco
authors.initials: SHC
authors.lastname: Sandes
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brazil.
authors.forename: Mayara Messias
authors.initials: MM
authors.lastname: Oliveira
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brazil.
authors.forename: Cristiano Villela
authors.initials: CV
authors.lastname: Dias
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brazil.
authors.forename: Samuel
authors.initials: S
authors.lastname: de Carvalho Silva
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brazil.
authors.forename: Ana Paula Trovatti
authors.initials: APT
authors.lastname: Uetanabaro
authors.affiliation: Instituto de Lactología Industrial, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Vinderola
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, Brazil. jnicoli@icb.ufmg.br.
authors.forename: Jacques Robert
authors.initials: JR
authors.lastname: Nicoli
mesh_terms: D000818:Animals; D000898:Antibiosis; D005260:Female; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000382:microbiology; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D019660:Malvaceae / Q000382:microbiology*; D051379:Mice; D019936:Probiotics / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000502:physiology
chemical_list: D019344:Lactic Acid
keywords: Infectious challenge; Lactobacillus plantarum; Probiotic selection; Salmonella Typhimurium; “Cupuaçu” fermentation