title: 10-Gingerol, a Phytochemical Derivative from "Tongling White Ginger", Inhibits Cervical Cancer: Insights into the Molecular Mechanism and Inhibitory Targets.
abstract: With the aim of evaluating anticancerous activities of 10-gingerol (10-G) against HeLa cells, it was purified and identified from "Tongling white ginger" by HSCCC, UPLC-TOF-MS/MS, and NMR analysis, respectively. 10-G inhibited the proliferation of HeLa cells at IC50(29.19 μM) and IC80(50.87 μM) with altered cell morphology, increased cytotoxicity, and arrested cell cycle in the G0/G1 phase. Most cell cycle related genes and protein expression significantly decreased, followed by a slight decrease in a few without affecting cyclin B1 and cyclin E1 (protein). Both death receptors significantly up-regulated and activated apoptosis indicators (caspase family). Furthermore, significant changes in mitochondria-dependent pathway markers were observed and led to cell death. 10-G led to PI3K/AKT inhibition and AMPK activation to induce mTOR-mediated cell apoptosis in HeLa cells. These results can be an asset to exploit 10-G with other medicinal plant derivatives for future applications.
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology , Hefei 230009, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology , Hefei 230009, People's Republic of China.
authors.forename: Kiran
authors.initials: K
authors.lastname: Thakur
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology , Hefei 230009, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology , Hefei 230009, People's Republic of China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhang
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology , Hefei 230009, People's Republic of China.|Agricultural and Forestry Specialty Food Processing Industry Technological Innovation Strategic Alliance of Anhui Province , Hefei 230009, People's Republic of China.
authors.forename: Zhao-Jun
authors.identifier: http://orcid.org/0000-0003-1729-209X
authors.initials: ZJ
authors.lastname: Wei
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002396:Catechols / Q000737:chemistry / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D005233:Fatty Alcohols / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D020939:Zingiber officinale / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology
chemical_list: D000970:Antineoplastic Agents; D002396:Catechols; D019927:Cyclin E; D005233:Fatty Alcohols; D010936:Plant Extracts; C007845:gingerol; D019869:Phosphatidylinositol 3-Kinases
keywords: 10-gingerol; HeLa cells; PI3K/AKT/AMPK/mTOR pathways; Tongling white ginger; apoptosis