title: Impact of plant matrix polysaccharides on cellulose produced by surface-tethered cellulose synthases.
abstract: Surface immobilized BcsA-B cellulose synthases synthesize crystalline cellulose II under in vitro conditions and were used to explore the interaction between cellulose and hemicelluloses and pectin. The morphology of the cellulose microfibrils changed in the presence of xyloglucan and glucomannan, while pectin did not significantly impact morphology. X-ray diffractometry and FT-IR spectroscopy indicated that crystal size and crystallinity were significantly affected by xyloglucan and glucomannan but not altered by pectin. Glucomannan had the most significant impact on the structure of cellulose and inhibits crystallization. The presence of xyloglucan and glucomannan prevents the proper assembly of cellulose microfibrils and changes the crystalline properties of cellulose II in in vitro conditions, but did not have any impact on cellulose allomorph.
authors.affiliation: Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, United States.
authors.forename: Snehasish
authors.initials: S
authors.lastname: Basu
authors.affiliation: Center for Membrane Biology, Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, United States.
authors.forename: Okako
authors.initials: O
authors.lastname: Omadjela
authors.affiliation: Center for Membrane Biology, Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, United States.
authors.forename: Jochen
authors.initials: J
authors.lastname: Zimmer
authors.affiliation: Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, United States. Electronic address: jcatchmark@engr.psu.edu.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Catchmark
mesh_terms: D002473:Cell Wall; D002482:Cellulose / Q000737:chemistry*; D005964:Glucosyltransferases / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011134:Polysaccharides; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase
keywords: Cellulose synthase; Crystal size; Glucomannan; Plant matrix polysaccharides; Surface immobilization; Xyloglucan