title: Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells.
abstract: Cellular metabolism has recently been identified as an important regulator of cell identity, with several adult stem cell populations having been observed to undergo a shift in metabolism during important changes in cell state, such as during the shift from quiescence to proliferation. In this chapter, a method to characterize the metabolism of quiescent skeletal muscle stem cells is presented. This technique will allow for the comparison of quiescent muscle stem cells isolated from two or more different mouse models.
authors.affiliation: Stem Cell Metabolism & Regenerative Medicine Group, Basic & Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, Parkville, VIC, 3010, Australia. jgryall@gmail.com.
authors.forename: James G
authors.initials: JG
authors.lastname: Ryall
mesh_terms: D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques; D018929:Cell Culture Techniques; D002469:Cell Separation; D004734:Energy Metabolism; D006019:Glycolysis; D008928:Mitochondria / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology*; D010085:Oxidative Phosphorylation; D016192:Resting Phase, Cell Cycle; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
keywords: Glycolysis; Metabolism; Mitochondria; Oxidative phosphorylation; Satellite cells