title: Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells.
abstract: Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types.
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences; Second Faculty of Medicine, Charles University, Prague.
authors.forename: Marketa
authors.initials: M
authors.lastname: Bacakova
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences; Second Faculty of Medicine, Charles University, Prague.
authors.forename: Julia
authors.initials: J
authors.lastname: Pajorova
authors.affiliation: InStar Technologies, Liberec.
authors.forename: Denisa
authors.initials: D
authors.lastname: Stranska
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences; Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hadraba
authors.affiliation: Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Lopot
authors.affiliation: Department of Chemistry and Physics of Surfaces and Biointerfaces, Institute of Macromolecular Chemistry.
authors.forename: Tomas
authors.initials: T
authors.lastname: Riedel
authors.affiliation: Department of Chemistry and Physics of Surfaces and Biointerfaces, Institute of Macromolecular Chemistry.
authors.forename: Eduard
authors.initials: E
authors.lastname: Brynda
authors.affiliation: Department of Composites and Carbon Materials, Institute of Rock Structure and Mechanics, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Margit
authors.initials: M
authors.lastname: Zaloudkova
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences.
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
mesh_terms: D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005353:Fibronectins; D011108:Polymers; D005337:Fibrin; D003094:Collagen
keywords: collagen; fibrin; nanocoating; nanofibers; skin cells; skin-tissue engineering