title: Respiratory Proteomics Today: Are Technological Advances for the Identification of Biomarker Signatures Catching up with Their Promise? A Critical Review of the Literature in the Decade 2004-2013.
abstract: To improve the knowledge on a variety of severe disorders, research has moved from the analysis of individual proteins to the investigation of all proteins expressed by a tissue/organism. This global proteomic approach could prove very useful: (i) for investigating the biochemical pathways involved in disease; (ii) for generating hypotheses; or (iii) as a tool for the identification of proteins differentially expressed in response to the disease state. Proteomics has not been used yet in the field of respiratory research as extensively as in other fields, only a few reproducible and clinically applicable molecular markers, which can assist in diagnosis, having been currently identified. The continuous advances in both instrumentation and methodology, which enable sensitive and quantitative proteomic analyses in much smaller amounts of biological material than before, will hopefully promote the identification of new candidate biomarkers in this area. The aim of this report is to critically review the application over the decade 2004-2013 of very sophisticated technologies to the study of respiratory disorders. The observed changes in protein expression profiles from tissues/fluids of patients affected by pulmonary disorders opens the route for the identification of novel pathological mediators of these disorders.
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/B, Pavia 27100, Italy. simona.viglio@unipv.it.
authors.forename: Simona
authors.initials: S
authors.lastname: Viglio
authors.affiliation: Department of Pulmonology, Leiden University Medical Center, Leiden 2333, The Netherlands. J.Stolk@lumc.nl.
authors.forename: Jan
authors.initials: J
authors.lastname: Stolk
authors.affiliation: Department of Biology and Biotechnologies, Biochemistry Unit, University of Pavia, Via Taramelli 3/B, Pavia 27100, Italy. piadarol@unipv.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Iadarola
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/B, Pavia 27100, Italy. serena.giuliano@unipv.it.|Faculty of Science "Parc Valrose", University of Nice "Sophia Antipolis", FRE 3472 CNRS, LP2M Nice, France. serena.giuliano@unipv.it.
authors.forename: Serena
authors.initials: S
authors.lastname: Giuliano
authors.affiliation: Department of Molecular Medicine, Division of Pneumology, University of Pavia & IRCCS Policlinico San Matteo, Via Taramelli 5, Pavia 27100, Italy. m.luisetti@smatteo.pv.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Luisetti
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/B, Pavia 27100, Italy. roberta.salvini@unipv.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: Salvini
authors.affiliation: Department of Biology and Biotechnologies, Biochemistry Unit, University of Pavia, Via Taramelli 3/B, Pavia 27100, Italy. fummar07@unipv.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Fumagalli
authors.affiliation: Department of Molecular Medicine, Biochemistry Unit, University of Pavia, Via Taramelli 3/B, Pavia 27100, Italy. abardoni@unipv.it.
authors.forename: Anna
authors.initials: A
authors.lastname: Bardoni
keywords: nano-LC-MS/MS; proteomics; respiratory diseases