title: Osteogenesis evaluation of duck's feet-derived collagen/hydroxyapatite sponges immersed in dexamethasone.
abstract: BACKGROUND
  The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone.
  
  
  METHODS
  The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(-)DC and Dex(-)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(-)DC, Dex(-)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR).
  
  
  RESULTS
  In this study, we assessed osteogenic differentiation of BMSCs on Duck's feet-derived collagen (DC)/HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days.
  
  
  CONCLUSIONS
  From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
authors.forename: Yeon Ji
authors.initials: YJ
authors.lastname: Kook
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
authors.forename: Dae Hoon
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Song
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
authors.forename: Nirmalya
authors.initials: N
authors.lastname: Tripathy
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
authors.forename: Yoo Shin
authors.initials: YS
authors.lastname: Jeon
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
authors.forename: Ha Yan
authors.initials: HY
authors.lastname: Jeon
authors.affiliation: 3B's Research Group -Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimaraes, Portugal.
authors.forename: Joaquim M
authors.initials: JM
authors.lastname: Oliveira
authors.affiliation: 3B's Research Group -Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimaraes, Portugal.
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
keywords: Bone regeneration; Dexamethasone; Duck’s feet-derived collagen; Hydroxyapatite; Osteogenesis; Sponges