title: Effect of ammonia on the gene expression levels of the freshwater cyclopoid Eucyclops serrulatus.
abstract: Ammonia pollution is a critical issue in Europe, since more than half of the European freshwater bodies actually fail to meet EU quality standards for this chemical. In this study, the response of stress-related genes to a sublethal ammonia concentration has been investigated in the adults of the freshwater cyclopoid Eucyclops serrulatus. Two short-term exposures (12h and 24h) at 12mg/L NH4+have been tested. Results indicate that 12mg/L NH4+causes a significant increase in the expression of some proteins, namely CAT, HSP90 and HSP40, suggesting an activation of the protecting antioxidant system after both 12h and 24h.
authors.affiliation: Institute of Ecosystem Study (ISE), CNR, Sesto Fiorentino, Firenze, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Di Lorenzo
authors.affiliation: Institute of Ecosystem Study (ISE), CNR, Sesto Fiorentino, Firenze, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Melita
authors.affiliation: Institute of Ecosystem Study (ISE), CNR, Sesto Fiorentino, Firenze, Italy; Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Cifoni
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: Diana M P
authors.initials: DMP
authors.lastname: Galassi
authors.affiliation: Institute of Ecosystem Study (ISE), CNR, Sesto Fiorentino, Firenze, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Iannucci
authors.affiliation: Department Agrifood Production and Environmental Sciences (DISPAA), Sesto Fiorentino, Firenze, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Biricolti
authors.affiliation: Department Agrifood Production and Environmental Sciences (DISPAA), Sesto Fiorentino, Firenze, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Gori
authors.affiliation: Institute of Ecosystem Study (ISE), CNR, Sesto Fiorentino, Firenze, Italy. Electronic address: baratti@ise.cnr.it.
authors.forename: Mariella
authors.initials: M
authors.lastname: Baratti
mesh_terms: D000641:Ammonia / Q000633:toxicity*; D000818:Animals; D002374:Catalase / Q000235:genetics; D033342:Copepoda / Q000187:drug effects* / Q000235:genetics; D004784:Environmental Monitoring; D005618:Fresh Water / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects*; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D007558:Italy; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D050956:HSP40 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D000641:Ammonia; D002374:Catalase
keywords: Ammonia; Copepoda; Freshwater; qRT-PCR