title: A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex-Peptide Monomers by Flow Cytometry.
abstract: Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage.
authors.affiliation: Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University and German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ) Partner Site Tuebingen , Tuebingen , Germany.
authors.forename: P Anoop
authors.initials: PA
authors.lastname: Chandran
authors.affiliation: Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University and German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ) Partner Site Tuebingen , Tuebingen , Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Heidu
authors.affiliation: Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University and German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ) Partner Site Tuebingen , Tuebingen , Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Zelba
authors.affiliation: Center for Clinical Transfusion Medicine GmbH, University Hospital , Tuebingen , Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Schmid-Horch
authors.affiliation: Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University and German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ) Partner Site Tuebingen , Tuebingen , Germany.
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Rammensee
authors.affiliation: Department of Dermatology, University Hospital , Zürich , Switzerland.
authors.forename: Steve
authors.initials: S
authors.lastname: Pascolo
authors.affiliation: Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University and German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ) Partner Site Tuebingen , Tuebingen , Germany.
authors.forename: Cécile
authors.initials: C
authors.lastname: Gouttefangeas
keywords: MHC–peptide monomers; UV-peptide exchange; antigen-specific T cells; multimers; quality control