title: Determination of Mycotoxin Production of Fusarium Species in Genetically Modified Maize Varieties by Quantitative Flow Immunocytometry.
abstract: Levels of mycotoxins produced byFusariumspecies in genetically modified (GM) and near-isogenic maize, were determined using multi-analyte, microbead-based flow immunocytometry with fluorescence detection, for the parallel quantitative determination of fumonisin B1, deoxynivalenol, zearalenone, T-2, ochratoxin A, and aflatoxin B1. Maize varieties included the genetic eventsMON 810andDAS-59122-7, and their isogenic counterparts. Cobs were artificially infested byF. verticillioidesandF. proliferatumconidia, and containedF. graminearumandF. sporotrichoidesnatural infestation. The production of fumonisin B1 and deoxynivalenol was substantially affected in GM maize lines:F. verticillioides, with the addition ofF. graminearumandF. sporotrichoides, produced significantly lower levels of fumonisin B1 (~300 mg·kg-1) inDAS-59122-7than in its isogenic line (~580 mg·kg-1), whileF. proliferatum, in addition toF. graminearumandF. sporotrichoides, produced significantly higher levels of deoxynivalenol (~18 mg·kg-1) inMON 810than in its isogenic line (~5 mg·kg-1).Fusarium verticillioides, withF. graminearumandF. sporotrichoides, produced lower amounts of deoxynivalenol and zearalenone thanF. proliferatum, withF. graminearumandF. sporotrichoides. T-2 toxin production remained unchanged when considering the maize variety. The results demonstrate the utility of the Fungi-Plex™ quantitative flow immunocytometry method, applied for the high throughput parallel determination of the target mycotoxins.
authors.affiliation: Agro-Environmental Research Institute, National Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. banati.hajnalka@naik.hu.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Bánáti
authors.affiliation: Agro-Environmental Research Institute, National Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. b.darvas@cfri.hu.
authors.forename: Béla
authors.initials: B
authors.lastname: Darvas
authors.affiliation: Soft Flow Hungary R&D Ltd., Ürögi fasor 2/A, H-7634 Pécs, Hungary. szilvia.toth@softflow.com.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Fehér-Tóth
authors.affiliation: Soft Flow Hungary R&D Ltd., Ürögi fasor 2/A, H-7634 Pécs, Hungary. arpad.czeh@softflow.com.
authors.forename: Árpád
authors.initials: Á
authors.lastname: Czéh
authors.affiliation: Agro-Environmental Research Institute, National Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. a.szekacs@cfri.hu.
authors.forename: András
authors.initials: A
authors.lastname: Székács
mesh_terms: D005434:Flow Cytometry; D005516:Food Microbiology; D005670:Fusarium / Q000145:classification / Q000378:metabolism*; D057166:High-Throughput Screening Assays; D009183:Mycotoxins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D003313:Zea mays / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*
chemical_list: D009183:Mycotoxins
keywords: DAS-59122-7; MON 810; T-2; aflatoxin B1; deoxynivalenol; flow cytometry; fumonisin B1; immunoanalysis; maize; ochratoxin A; zearalenone