title: Antimicrobial activity of biogenically produced spherical Se-nanomaterials embedded in organic material against Pseudomonas aeruginosa and Staphylococcus aureus strains on hydroxyapatite-coated surfaces.
abstract: In an effort to prevent the formation of pathogenic biofilms on hydroxyapatite (HA)-based clinical devices and surfaces, we present a study evaluating the antimicrobial efficacy of Spherical biogenic Se-Nanostructures Embedded in Organic material (Bio Se-NEMO-S) produced by Bacillus mycoides SelTE01 in comparison with two different chemical selenium nanoparticle (SeNP) classes. These nanomaterials have been studied as potential antimicrobials for eradication of established HA-grown biofilms, for preventing biofilm formation on HA-coated surfaces and for inhibition of planktonic cell growth of Pseudomonas aeruginosa NCTC 12934 and Staphylococcus aureus ATCC 25923. Bio Se-NEMO resulted more efficacious than those chemically produced in all tested scenarios. Bio Se-NEMO produced by B. mycoides SelTE01 after 6 or 24 h of Na2SeO3exposure show the same effective antibiofilm activity towards both P. aeruginosa and S. aureus strains at 0.078 mg ml-1(Bio Se-NEMO6) and 0.3125 mg ml-1(Bio Se-NEMO24). Meanwhile, chemically synthesized SeNPs at the highest tested concentration (2.5 mg ml-1) have moderate antimicrobial activity. The confocal laser scanning micrographs demonstrate that the majority of the P. aeruginosa and S. aureus cells exposed to biogenic SeNPs within the biofilm are killed or eradicated. Bio Se-NEMO therefore displayed good antimicrobial activity towards HA-grown biofilms and planktonic cells, becoming possible candidates as new antimicrobials.
authors.affiliation: Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Dr NW, Calgary, AB, T2N 1N4, Canada.
authors.forename: Elena
authors.initials: E
authors.lastname: Piacenza
authors.affiliation: Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Dr NW, Calgary, AB, T2N 1N4, Canada.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Presentato
authors.affiliation: Environmental Microbiology Laboratory, Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Zonaro
authors.affiliation: Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Dr NW, Calgary, AB, T2N 1N4, Canada.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Lemire
authors.affiliation: Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Dr NW, Calgary, AB, T2N 1N4, Canada.
authors.forename: Marc
authors.initials: M
authors.lastname: Demeter
authors.affiliation: Environmental Microbiology Laboratory, Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vallini
authors.affiliation: Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Dr NW, Calgary, AB, T2N 1N4, Canada.
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Turner
authors.affiliation: Environmental Microbiology Laboratory, Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lampis
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism*; D001407:Bacillus / Q000378:metabolism*; D001672:Biocompatible Materials; D018441:Biofilms / Q000187:drug effects; D017886:Durapatite; D004783:Environmental Microbiology; D053768:Metal Nanoparticles; D009942:Organometallic Compounds / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D012643:Selenium / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D009942:Organometallic Compounds; D017886:Durapatite; D012643:Selenium