title: SFRP2 enhanced the adipogenic and neuronal differentiation potentials of stem cells from apical papilla.
abstract: Dental tissue-derived mesenchymal stem cells (MSCs) are easily obtained and considered as a favorable cell source for tissue engineering, but the regulation of direct differentiation is unknown, which restricts their application. The present study investigated the effect of SFRP2, a Wnt signaling modulator, on MSC differentiation using stem cells from apical papilla (SCAPs). The cells were cultured in specific inducing medium for adipogenic, neurogenic, or chondrogenic differentiation. Over-expression of SFRP2 via retroviral infection enhanced the adipogenic and neurogenic differentiation of SCAPs. While inhibit of Wnt pathway by IWR1-endo could enhance the neurogenic differentiation potentials of SCAPs, similar with the function of SFRP2. In addition, over-expression of SFRP2 up-regulated the expression of stemness-related genes SOX2 and OCT4. Furthermore, SOX2 and OCT4 expression was significantly inhibited after lentiviral silencing of SFRP2 in SCAPs. Therefore, our results suggest that SFRP2 enhances the adipogenic and neurogenic differentiation potentials of SCAPs by up-regulating SOX2 and OCT4. Moreover, the effect of SFRP2 in neurogenic differentiation of SCAPs maybe also associated with Wnt inhibition. Our results provided useful information about the molecular mechanism underlying directed differentiation in dental tissue-derived MSCs.
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy, Capital Medical University School of Stomatology, Beijing, China.|Department of Implant Dentistry, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Lin
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Dong
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy, Capital Medical University School of Stomatology, Beijing, China.|Department of Pediatric Dentistry, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Diao
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy, Capital Medical University School of Stomatology, Beijing, China.|Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.|Department of Stomatology, Beijing Children's Hospital, Capital Medical University, Beijing, China.
authors.forename: Guoxia
authors.initials: G
authors.lastname: Yu
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Implant Dentistry, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Fan
mesh_terms: D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000293:Adolescent; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D020219:Chondrogenesis / Q000187:drug effects; D003771:Dental Papilla / Q000166:cytology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D055815:Young Adult; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D008565:Membrane Proteins; D050814:Octamer Transcription Factor-3; D012333:RNA, Messenger; D055748:SOXB1 Transcription Factors; C494173:POU5F1 protein, human; C109618:SFRP2 protein, human; D000094742:Secreted Frizzled-Related Proteins
keywords: SFRP2; adipogenic differentiation; neurogenic differentiation; stem cells from apical papilla (SCAPs); stemness