title: [Mechanism of Mitochondria-mediated Pathway in the Platelet Apoptosis Resulted from Immune Bone Marrow Failure].
abstract: OBJECTIVE
  To explore the mechamisms of mitochondria-mediated pathway in apoptosis of platelets resulted from in immune induced bone marrow failure.
  
  
  METHODS
  Thirty C57BL/6 mice were randomly divided into 3 groups (10 mice in each group): normal group, model group, cyclosporine A(CsA) group. Mouse model of immune bone marrow failure were established. After mouse model was successfully established, the mice in normal group and model group were given saline orally, the mice in CsA group was treated with CsA orally. Blood routine examination of mice in each group was performed by automatic blood cell analyzer; the mitochondrial membrane potential(ΔΨm), cytochrome C(Cyt C), phosphatidylserine (PS), Ca2+were measured by flow cytometry; expression of BAX, BAK, caspase-3, caspase-8, caspase-9 was detected by using Western blot method, the changes of bone marrow platelet ultrastructure were observed under transmission electron microscope.
  
  
  RESULTS
  Compared with normal group, the platelet count of model group decreased significantly, while the level of ΔΨm, caspase-3, caspase-8, caspase-9 significantly decreased, the level of Cyt C, PS, Ca2+, BAX, BAK increased significantly (P<0.05). Compared with the model group, the platelet count of CsA group increased obviously, while the level of ΔΨm, caspase-3, caspase-8, caspase-9 of CsA group increased significantly, the level of Cyt C, PS, Ca2+, BAX, BAK of CsA group decreased significantly (P<0.05). Electron microscopy showed that compared with the model group, platelet damage in CsA group were alleviated.
  
  
  CONCLUSIONS
  mitochondrial pathway plays an important role in the reduction of platelet resulted from immune bone marrow failure.
authors.affiliation: Department of Hematology, Baoshan Hospital of Integrated Tradtional Chinese and Western Medicine, Shanghai 201900, China.
authors.forename: Le-Min
authors.initials: LM
authors.lastname: Xia
authors.affiliation: Department of Hematology, Baoshan Hospital of Integrated Tradtional Chinese and Western Medicine, Shanghai 201900, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhen
authors.affiliation: Department of Hematology, Baoshan Hospital of Integrated Tradtional Chinese and Western Medicine, Shanghai 201900, China.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Baoshan Hospital of Integrated Tradtional Chinese and Western Medicine, Shanghai 201900, China.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Jiang
authors.affiliation: Department of Hematology, Baoshan Hospital of Integrated Tradtional Chinese and Western Medicine, Shanghai 201900, China.
authors.forename: Le-Le
authors.initials: LL
authors.lastname: Cui
authors.affiliation: Department of Hematology, Baoshan Hospital of Integrated Tradtional Chinese and Western Medicine, Shanghai 201900, China. E-mail: lmh021009@163.com.
authors.forename: Mei-Hong
authors.initials: MH
authors.lastname: Luo
mesh_terms: D000818:Animals; D017209:Apoptosis; D001792:Blood Platelets; D001853:Bone Marrow; D053148:Caspase 3; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000502:physiology*; D011897:Random Allocation
chemical_list: D053148:Caspase 3