title: Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans.
abstract: Angular leaf spot (ALS) and powdery mildew (PWM) are two important fungi diseases causing significant yield losses in common beans. In this study, a new genetic linkage map was constructed using single sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), in a segregating population derived from the AND 277 x SEA 5 cross, with 105 recombinant inbred lines. Phenotypic evaluations were performed in the greenhouse to identify quantitative trait loci (QTLs) associated with resistance by means of the composite interval mapping analysis. Four QTLs were identified for ALS resistance. The QTL ALS11AS, linked on the SNP BAR 5054, mapped on chromosome Pv11, showed the greatest effect (R2 = 26.5%) on ALS phenotypic variance. For PWM resistance, two QTLs were detected, PWM2AS and PWM11AS, on Pv2 and Pv11, explaining 7% and 66% of the phenotypic variation, respectively. Both QTLs on Pv11 were mapped on the same genomic region, suggesting that it is a pleiotropic region. The present study resulted in the identification of new markers closely linked to ALS and PWM QTLs, which can be used for marker-assisted selection, fine mapping and positional cloning.
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Denis
authors.initials: D
authors.lastname: Bassi
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Boris
authors.initials: B
authors.lastname: Briñez
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Juliana Santa
authors.initials: JS
authors.lastname: Rosa
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Paula Rodrigues
authors.initials: PR
authors.lastname: Oblessuc
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Caléo Panhoca de
authors.initials: CP
authors.lastname: Almeida
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Stella Maris
authors.initials: SM
authors.lastname: Nucci
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Larissa Chariel Domingos da
authors.initials: LCDD
authors.lastname: Silva
authors.affiliation: Centro de Grãos e Fibras, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Alisson Fernando
authors.initials: AF
authors.lastname: Chiorato
authors.affiliation: Centro Nacional de Pesquisa de Arroz e Feijão, EMBRAPA, Santo Antônio de Goiás, GO, Brazil.
authors.forename: Rosana Pereira
authors.initials: RP
authors.lastname: Vianello
authors.affiliation: Departamento de Fitopatologia, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Universidade de São Paulo (USP), Piracicaba, SP, Brazil.
authors.forename: Luis Eduardo Aranha
authors.initials: LEA
authors.lastname: Camargo
authors.affiliation: Department of Agriculture and Environmental Sciences, Tennessee State University, Nashville, TN, USA.
authors.forename: Matthew Wohlgemuth
authors.initials: MW
authors.lastname: Blair
authors.affiliation: Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), Campinas, SP, Brazil.
authors.forename: Luciana Lasry
authors.initials: LL
authors.lastname: Benchimol-Reis