title: Chitin and Stress Induced Protein Kinase Activation.
abstract: The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark.
authors.forename: Chandra
authors.initials: C
authors.lastname: Kenchappa
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark.
authors.forename: Raquel Azevedo
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark.
authors.forename: Simon
authors.initials: S
authors.lastname: Bressendorff
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Stanimirovic
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark.
authors.forename: Jakob
authors.initials: J
authors.lastname: Olsen
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Petersen
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, 2200, Copenhagen, Denmark. mundy@bio.ku.dk.
authors.forename: John
authors.initials: J
authors.lastname: Mundy
mesh_terms: D002686:Chitin / Q000494:pharmacology*; D004789:Enzyme Activation; D015151:Immunoblotting; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000378:metabolism*; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000187:drug effects* / Q000201:enzymology; D013312:Stress, Physiological; D013379:Substrate Specificity
chemical_list: D004676:Myelin Basic Protein; D010940:Plant Proteins; D002686:Chitin; D020928:Mitogen-Activated Protein Kinases
keywords: Acryloylated peptide substrates; In-gel assays; Myelin basic protein