title: The Salmonella effector SseJ disrupts microtubule dynamics when ectopically expressed in normal rat kidney cells.
abstract: Salmonella effector protein SseJ is secreted by Salmonella into the host cell cytoplasm where it can then modify host cell processes. Whilst host cell small GTPase RhoA has previously been shown to activate the acyl-transferase activity of SseJ we show here an un-described effect of SseJ protein production upon microtubule dynamism. SseJ prevents microtubule collapse and this is independent of SseJ's acyl-transferase activity. We speculate that the effects of SseJ on microtubules would be mediated via its known interactions with the small GTPases of the Rho family.
authors.affiliation: Department of Biology, Wentworth Way, University of York, York, United Kingdom.
authors.forename: Sally A
authors.initials: SA
authors.lastname: Raines
authors.affiliation: Department of Biology, Wentworth Way, University of York, York, United Kingdom.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hodgkinson
authors.affiliation: Technology Facility, Department of Biology, Wentworth Way, University of York, York, United Kingdom.
authors.forename: Adam A
authors.initials: AA
authors.lastname: Dowle
authors.affiliation: Department of Biology, Wentworth Way, University of York, York, United Kingdom.|Hull York Medical School, University of York, York, United Kingdom.
authors.forename: Paul R
authors.identifier: http://orcid.org/0000-0001-9123-8329
authors.initials: PR
authors.lastname: Pryor
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology* / Q000648:ultrastructure; D005786:Gene Expression Regulation; D044404:Genomic Islands; D015698:Genomic Library; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D008264:Macrophages / Q000382:microbiology / Q000648:ultrastructure; D008870:Microtubules / Q000382:microbiology* / Q000648:ultrastructure; D011485:Protein Binding; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D016415:Sequence Alignment; D015398:Signal Transduction; D020741:rho GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D000217:Acyltransferases; C000621224:RhoA protein, rat; D020741:rho GTP-Binding Proteins