title: Characterization and Antimicrobial Resistance of Salmonella Typhimurium Isolates from Clinically Diseased Pigs in Korea.
abstract: This study investigated the prevalence of Salmonella enterica serovar and antimicrobial resistance in Salmonella Typhimurium isolates from clinically diseased pigs collected from 2008 to 2014 in Korea. Isolates were also characterized according to the presence of antimicrobial resistance genes and pulsed-field gel electrophoresis patterns. Among 94 Salmonella isolates, 81 (86.2%) were identified as being of the Salmonella Typhimurium serotype, followed by Salmonella Derby (6 of 94, 6.4%), Salmonella 4,[5],12:i:- (4 of 94, 4.3%), Salmonella Enteritidis (2 of 94, 2.1%), and Salmonella Brandenburg (1 of 94, 1.1%). The majority of Salmonella Typhimurium isolates were resistant to tetracycline (92.6%), followed by streptomycin (88.9%) and ampicillin (80.2%). Overall, 96.3% of Salmonella Typhimurium isolates showed multidrug-resistant phenotypes and commonly harbored the resistance genes blaTEM(64.9%), flo (32.8%), aadA (55.3%), strA (58.5%), strB (58.5%), sulII (53.2%), and tetA (61.7%). The pulsed-field gel electrophoresis analysis of 45 Salmonella Typhimurium isolates from individual farms revealed 27 distinct patterns that formed one major and two minor clusters in the dendrogram analysis, suggesting that most of the isolates (91.1%) from diseased pigs were genetically related. These findings can assist veterinarians in the selection of appropriate antimicrobial agents to combat Salmonella Typhimurium infections in pigs. Furthermore, they highlight the importance of continuous surveillance of antimicrobial resistance and genetic status in Salmonella Typhimurium for the detection of emerging resistance trends.
authors.affiliation: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of Korea.|College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan 54596, Republic of Korea.
authors.forename: Sang-Ik
authors.initials: SI
authors.lastname: Oh
authors.affiliation: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of Korea.
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of Korea.
authors.forename: Myeongju
authors.initials: M
authors.lastname: Chae
authors.affiliation: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of Korea.
authors.forename: Ji-A
authors.initials: JA
authors.lastname: Jung
authors.affiliation: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of Korea.
authors.forename: Byungjae
authors.initials: B
authors.lastname: So
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan 54596, Republic of Korea.
authors.forename: Bumseok
authors.initials: B
authors.lastname: Kim
authors.affiliation: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of Korea.
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D008826:Microbial Sensitivity Tests; D056910:Republic of Korea; D012481:Salmonella Infections, Animal; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents
keywords: Antimicrobial resistance; Diseased pigs; Salmonella Typhimurium