title: Early osseointegration of implants with cortex-like TiO2coatings formed by micro-arc oxidation: A histomorphometric study in rabbits.
abstract: In our previous studies, a novel cortex-like TiO2coating was prepared on Ti surface through micro-arc oxidation (MAO) by using sodium tetraborate as electrolyte, and the effects of the coating on cell attachment were testified. This study aimed to investigate the effects of this cortex-like MAO coating on osseointegration. A sand-blasting and acid-etching (SLA) coating that has been widely used in clinical practice served as control. Topographical and chemical characterizations were conducted by scanning electron microscopy, energy dispersive X-ray spectrometer, X-ray diffraction, contact angle meter, and step profiler. Results showed that the cortex-like coating had microslots and nanopores and it was superhydrophilic, whereas the SLA surface was hydrophobic. The roughness of MAO was similar to that of SLA. The MAO and SLA implants were implanted into the femoral condyles of New Zealand rabbits to evaluate their in-vivo performance through micro-CT, histological analysis, and fluorescent labeling at the bone-implant interface four weeks after surgery. The micro-CT showed that the bone volume ratio and mean trabecular thickness were similar between MAO and SLA groups four weeks after implantation. Histological analysis and fluorescent labeling showed no significant differences in the bone-implant contact between the MAO and SLA surfaces. It was suggested that with micro/nanostructure and superhydrophilicity, the cortex-like MAO coating causes excellent osseointegration, holding a promise of an application to implant modification.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.|Stomatological Hospital of Dalian, Dalian, 116021, China.
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Zhou
authors.affiliation: School of Materials Science and Engineering, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Ya-da
authors.initials: YD
authors.lastname: Li
authors.affiliation: Stomatological Hospital of Dalian, Dalian, 116021, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Stomatological Hospital of Dalian, Dalian, 116021, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Wang
authors.affiliation: School and Hospital of Stomatology, Dalian Medical University, Dalian, 116044, China.
authors.forename: Guo-Wu
authors.initials: GW
authors.lastname: Ma
authors.affiliation: Implantology Center, Peking Union Medical College Hospital, Beijing, 100032, China.
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Su
authors.affiliation: School of Materials Science and Engineering, Dalian University of Technology, Dalian, 116024, China. minqi@dlut.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Qi
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China. shibin_dentist@126.com.|Department of Dental Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China. shibin_dentist@126.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D005269:Femur / Q000000981:diagnostic imaging* / Q000601:surgery; D020007:Implants, Experimental; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects*; D010084:Oxidation-Reduction; D011817:Rabbits; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
chemical_list: D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium
keywords: cortex-like structure; histomorphometry; micro-arc oxidation; osseointegration; titanium implant