title: Association between SLC11A1 (NRAMP1) polymorphisms and susceptibility to tuberculosis in Chinese Holstein cattle.
abstract: We investigated the associations between SLC11A1 polymorphisms and susceptibility to tuberculosis (TB) in Chinese Holstein cattle, using a case-control study of 136 animals that had positive reactions to TB tests and showed symptoms and 96 animals that had negative reactions to tests and showed no symptoms. Polymerase chain reaction (PCR) sequencing and the restriction fragment length polymorphism (RFLP) technique were used to detect and determine SLC11A1 polymorphisms. Association analysis identified significant correlations between SLC11A1 polymorphisms and susceptibility/resistance to TB, and two genetic markers for SLC11A1 were established using PCR-RFLP. Sequence alignment of SLC11A1 revealed seven single-nucleotide polymorphisms (SNPs). This is the first report of MaeII PCR-RFLP markers for the SLC11A1-SNP3 site and PstI PCR-RFLP markers for the SLC11A1-SNP5 and SLC11A1-SNP6 sites in Chinese Holstein cattle. Logistic regression analysis indicated that SLC11A1-SNP1, SLC11A1-SNP3, and SLC11A1-SNP5 were significantly associated with susceptibility/resistance to TB. Two genotypes of SLC11A1-SNP3 were susceptible to TB, whereas one genotype of SLC11A1-SNP1 and two genotypes of SLC11A1-SNP5 were resistant. Haplotype analysis showed that nine haplotypes were potentially resistant to TB. After Bonferroni correction, three of the haplotypes remained significantly associated with TB resistance. SLC11A1 is a useful candidate gene related to TB in Chinese Holstein cattle.
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Zhaochun
authors.initials: Z
authors.lastname: Teng
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Center for Animal Disease Control and Prevention of Yunnan Province, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Dong
authors.affiliation: Center for Animal Disease Control and Prevention of Yuxi City, Yunnan Province, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Chunlian
authors.initials: C
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chai
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China. Electronic address: 1464406866@qq.com.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Shu
authors.affiliation: College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, China. Electronic address: zyfkm@qq.com.
authors.forename: Yifang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000662:veterinary*; D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics*; D002417:Cattle; D002681:China; D003612:Dairying; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D015810:Linkage Disequilibrium; D016015:Logistic Models; D009163:Mycobacterium bovis / Q000472:pathogenicity*; D016017:Odds Ratio; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014380:Tuberculosis, Bovine / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1
keywords: PCR-RFLP; SLC11A1; SNP; Susceptibility; Tuberculosis