title: Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing.
abstract: Using a combination of phenotypic screening, genetic and statistical analyses, and high-throughput genome-wide sequencing, we have finely mapped a dominant Phytophthora resistance gene in soybean cultivar Wayao. Phytophthora root rot (PRR) caused by Phytophthora sojae is one of the most important soil-borne diseases in many soybean-production regions in the world. Identification of resistant gene(s) and incorporating them into elite varieties are an effective way for breeding to prevent soybean from being harmed by this disease. Two soybean populations of 191 F2individuals and 196 F7:8recombinant inbred lines (RILs) were developed to map Rps gene by crossing a susceptible cultivar Huachun 2 with the resistant cultivar Wayao. Genetic analysis of the F2population indicated that PRR resistance in Wayao was controlled by a single dominant gene, temporarily named RpsWY, which was mapped on chromosome 3. A high-density genetic linkage bin map was constructed using 3469 recombination bins of the RILs to explore the candidate genes by the high-throughput genome-wide sequencing. The results of genotypic analysis showed that the RpsWY gene was located in bin 401 between 4466230 and 4502773 bp on chromosome 3 through line 71 and 100 of the RILs. Four predicted genes (Glyma03g04350, Glyma03g04360, Glyma03g04370, and Glyma03g04380) were found at the narrowed region of 36.5 kb in bin 401. These results suggest that the high-throughput genome-wide resequencing is an effective method to fine map PRR candidate genes.
authors.affiliation: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.|The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.|The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.
authors.forename: Qibin
authors.initials: Q
authors.lastname: Ma
authors.affiliation: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.|The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Ren
authors.affiliation: Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen, 518086, People's Republic of China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Xia
authors.affiliation: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.|The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.
authors.forename: Enliang
authors.initials: E
authors.lastname: Song
authors.affiliation: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.|The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Agricultural Research Service, Crop Genetics Research Unit, United States Department of Agriculture, Stoneville, MS, 38776, USA.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Li
authors.affiliation: Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen, 518086, People's Republic of China.
authors.forename: Gengyun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China. hnian@scau.edu.cn.|The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China. hnian@scau.edu.cn.
authors.forename: Hai
authors.initials: H
authors.lastname: Nian
mesh_terms: D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D005799:Genes, Dominant; D017343:Genes, Plant; D008040:Genetic Linkage; D059014:High-Throughput Nucleotide Sequencing; D018895:Microsatellite Repeats; D010641:Phenotype; D010838:Phytophthora; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000235:genetics* / Q000382:microbiology