title: High throughput selection of antibiotic-resistant transgenic Arabidopsis plants.
abstract: Kanamycin resistance is the most frequently used antibiotic-resistance marker for Arabidopsis transformations, however, this method frequently causes escape of untransformed plants, particularly at the high seedling density during the selection. Here we developed a robust high-density selection method using top agar for Arabidopsis thaliana. Top agar effectively suppressed growth of untransformed wild-type plants on selection media at high density. Survival of the transformed plants during the selection were confirmed by production of green true leaves and expression of a firefly luciferase reporter gene. Top agar method allowed selection using a large amount of seeds in Arabidopsis transformation.
authors.affiliation: Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station 77843-2133, TX, USA. Electronic address: nyukihiro@tamu.edu.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nagashima
authors.affiliation: Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station 77843-2133, TX, USA; Molecular and Environmental Plant Science Program, Texas A&M University, College Station 77843-2133, TX, USA. Electronic address: koiwa@tamu.edu.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koiwa
mesh_terms: D000362:Agar / Q000737:chemistry* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D057166:High-Throughput Screening Assays; D007613:Kanamycin Resistance / Q000235:genetics*; D008156:Luciferases / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D000362:Agar; D008156:Luciferases
keywords: Antibiotic selection; Arabidopsis; Transformation