title: A simple LC-MS/MS method for the simultaneous quantification of resveratrol and its major phase II metabolites: Assessment of their urinary and biliary excretions in rats.
abstract: Trans-resveratrol (Res) is rapidly metabolized, extensively distributed into various tissues and mainly excreted by urine. The present study aimed to establish a simple LC-MS/MS method to simultaneously quantify Res and its major phase II metabolites (Res-3-O-β-d-glucuronide, R3G; Res-4'-O-β-d-glucuronide, R4'G; Res-resveratrol 3-sulfate, R3S; and Res-4'-sulfate, R4'S), and apply this method to assess their urinary and biliary excretions in rats. A simplified salting-out assisted liquid-liquid extraction (SALLE) strategy was developed to prepare samples with acetonitrile-methanol mixture (8:2, v/v) as extractant and ammonium acetate solution (10M) as salting-out reagent. The method validation demonstrated an acceptable recovery (>80%), good accuracy (85-115%), low deviation of detection (<15%) and no obvious matrix effect (<20%). Then the validated method was successfully applied to analyze the excretion of Res and its metabolites after intragastric administration of Res at 50mg/kg in rats. Only a minor proportion of Res (0.51nmol) and its metabolites (R3S, 35.8nmol; R4S, 0.25nmol; R3G, 142.3nmol; R4'G, 0.19nmol) were eliminated via bile, while the majority of Res (1670.2nmol), R3G (14,089.0nmol) and R3S (2975.6nmol) were excreted through urine. The major forms found in feces were Res and R3S, which were accumulated up to 241.8 and 250.8nmol, respectively. In summary, the SALLE technique simplified the samples preparation and could be well popularized, especially for those highly polar compounds in biosamples like urine, bile and feces, where various endogenous substances could significantly affect the extraction recovery and detection response.
authors.affiliation: Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Yu
authors.affiliation: Clinical Pharmacokinetics Research Laboratory, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Clinical Pharmacokinetics Research Laboratory, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.
authors.forename: Xijing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China. Electronic address: chengtianle007@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.affiliation: Clinical Pharmacokinetics Research Laboratory, China Pharmaceutical University, Nanjing, Jiangsu 210009, China; National Experimental Teaching Demonstration Center of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. Electronic address: ningli656@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D065669:Hepatobiliary Elimination; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism* / Q000493:pharmacokinetics* / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D000077185:Resveratrol
keywords: Conjugated metabolites; Excretion; LC–MS/MS; Trans-resveratrol