title: Highly Efficient Enzymatic Acylation of Dihydromyricetin by the Immobilized Lipase with Deep Eutectic Solvents as Cosolvent.
abstract: A novel deep eutectic solvent (DES)-DMSO cosolvent system has been, for the first time, successfully used as the reaction medium for the enzymatic acylation of dihydromyricetin (DMY) catalyzed by the immobilized lipase from Aspergillus niger (ANL). The cosolvent mixture, ChCl:Glycerol-DMSO (1:3, v/v) proved to be the optimal medium. With the newly developed cosolvent, the initial reaction rate of enzymatic acylation of DMY achieved 11.1 mM/h and the conversion of DMY was 91.6%. ANL@PD-MNPs is stable and recyclable in this cosolvent, offering 90% conversion rate after repeated use of 5 times. The lipid-solubility of DMY-16-acetate was 10 times higher than that of its raw materials DMY. The results showed that the DMY-16-acetate product exhibits good antioxidative activity. The present research illustrated that the use of DES-DMSO cosolvent may become a feasible alternative for the synthesis of DMY ester.
authors.affiliation: Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.|Department of Food Science, Foshan University , No. 18 Jiangwan Yi Road, Foshan 528000, China.
authors.forename: Shi-Lin
authors.identifier: http://orcid.org/0000-0002-7702-5279
authors.initials: SL
authors.lastname: Cao
authors.affiliation: Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Deng
authors.affiliation: Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Xu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
authors.forename: Zi-Xuan
authors.initials: ZX
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
authors.forename: Jian
authors.identifier: http://orcid.org/0000-0002-3033-7785
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
authors.forename: Xue-Hui
authors.initials: XH
authors.lastname: Li
authors.affiliation: Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.|School of Chemistry and Chemical Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
authors.forename: Min-Hua
authors.initials: MH
authors.lastname: Zong
authors.affiliation: Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.|State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , No. 381 Wushan Road, Guangzhou 510640, China.
authors.forename: Wen-Yong
authors.identifier: http://orcid.org/0000-0003-3474-3446
authors.initials: WY
authors.lastname: Lou
mesh_terms: D000215:Acylation; D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D044948:Flavonols / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D044948:Flavonols; D005656:Fungal Proteins; D008049:Lipase; C472036:dihydromyricetin
keywords: Aspergillus niger lipase; acylation; antioxidant ability; deep eutectic solvent; dihydromyricetin