title: Reconstruction of molecular phylogeny of closely relatedAmorphophallusspecies of India using plastid DNA marker and fingerprinting approaches.
abstract: Plastid DNA markers sequencing and DNA fingerprinting approaches were used and compared for resolving molecular phylogeny of closely related, previously unexploredAmorphophallusspecies of India. The utility of individual plastid markers namelyrbcL,matK,trnH-psbA,trnLC-trnLD, their combined dataset and two fingerprinting techniques viz. RAPD and ISSR were tested for their efficacy to resolvesAmorphophallusspecies into three sections specific clades namelyRhaphiophallus,ConophallusandAmorphophallus. In the present study, sequences of these four plastid DNA regions as well as RAPD and ISSR profiles of 16Amorphophallusspecies together with six varieties of two species were generated and analyzed. Maximum likelihood and Bayesian Inference based construction of phylogenetic trees indicated that among the four plastid DNA regions tested individually and their combined dataset,rbcLwas found best suited for resolving closely relatedAmorphophallusspecies into section specific clades. When analyzed individually,rbcLexhibited better discrimination ability thanmatK,trnH-psbA,trnLC-trnLDand combination of all four tested plastid markers. Among two fingerprinting techniques used, the resolution ofAmorphophallusspecies using RAPD was better than ISSR and combination of RAPD +ISSR and in congruence with resolution based onrbcL.
authors.affiliation: Department of Botany, Shivaji University, Vidyanagar, Kolhapur, Maharashtra India.
authors.forename: Avinash R
authors.initials: AR
authors.lastname: Gholave
authors.affiliation: Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra India.|School of Nanoscience and Biotechnology, Shivaji University, Kolhapur, Maharashtra India.
authors.forename: Kiran D
authors.initials: KD
authors.lastname: Pawar
authors.affiliation: Department of Botany, Shivaji University, Vidyanagar, Kolhapur, Maharashtra India.
authors.forename: Shrirang R
authors.initials: SR
authors.lastname: Yadav
authors.affiliation: Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra India.
authors.forename: Vishwas A
authors.initials: VA
authors.lastname: Bapat
authors.affiliation: Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra India.
authors.forename: Jyoti P
authors.initials: JP
authors.lastname: Jadhav
keywords: Amorphophallus; Bayesian inference; DNA finger printing; Maximum likelihood; Molecular phylogeny; matK; rbcL