title: Selenium supplementation modulates apoptotic processes in thyroid follicular cells.
abstract: Selenium (Se) is an essential micronutrient modulating several physiopathological processes in the human body. The aim of the study is to characterize the molecular effects determined by Se-supplementation in thyroid follicular cells, using as model the well-differentiated rat thyroid follicular cell line FRTL5. Experiments have been performed to evaluate the effects of Se on cell growth, mortality and proliferation and on modulation of pro- and antiapoptotic pathways. The results indicate that Se-supplementation improves FRTL5 growth rate. Furthermore, Se reduces the proportion of cell death and modulates both proapoptotic (p53 and Bim) and antiapoptotic (NF-kB and Bcl2) mRNA levels. In addition, incubation with high doses of Na-Se might prevent the ER-stress apoptosis induced by tunicamycin, as assessed by membrane integrity maintenance, reduction in caspase 3/7 activities, and reduction in Casp-3 and PARP cleavage. Taken together, these results provide molecular evidences indicating the role of Se supplementation on cell death and apoptosis modulation in thyroid follicular cells. These observations may be useful to understand the effects of this micronutrient on the physiopathology of the thyroid gland. © 2016 BioFactors, 43(3):415-423, 2017.
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Immacolata C
authors.initials: IC
authors.lastname: Nettore
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Emma
authors.initials: E
authors.lastname: De Nisco
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Silvio
authors.initials: S
authors.lastname: Desiderio
authors.affiliation: Dipartimento di Scienze Mediche Traslazionali, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: Passaro
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Maione
authors.affiliation: I.O.S. and COLEMAN S.r.l, Naples, 80100, Italy.
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Negri
authors.affiliation: Dipartimento di Scienze Mediche Traslazionali, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Albano
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Pivonello
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Pivonello
authors.affiliation: Dipartimento di Scienze Mediche Traslazionali, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Portella
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale del CNR (IEOS-CNR) "G. Salvatore", Napoli, 80131, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Ungaro
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Colao
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli "Federico II", Napoli, 80131, Italy.
authors.forename: Paolo E
authors.initials: PE
authors.lastname: Macchia
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D000072224:Bcl-2-Like Protein 11 / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000494:pharmacology*; D015398:Signal Transduction; D000072637:Thyroid Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D000072224:Bcl-2-Like Protein 11; C000617196:Bcl2 protein, rat; C000606778:Bcl2l11 protein, rat; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D014415:Tunicamycin; D011065:Poly(ADP-ribose) Polymerases; C505410:Casp3 protein, rat; D053148:Caspase 3; D053179:Caspase 7; D012643:Selenium
keywords: Hashimoto's thyroiditis; apoptosis; autoimmunity; selenium