title: Expanding the genetic code of Mus musculus.
abstract: Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Nɛ-acetyl-lysine. Stable integration of transgenes encoding an engineered Nɛ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPylpair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.
authors.affiliation: Department of Physiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
authors.forename: Songmi
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
authors.forename: Aerin
authors.identifier: 0000-0002-8704-0288
authors.initials: A
authors.lastname: Yang
authors.affiliation: Department of Physiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
authors.forename: Soonjang
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, Yonsei Laboratory Animal Research Center, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Han-Woong
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Physiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
authors.forename: Chan Bae
authors.initials: CB
authors.lastname: Park
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Park
mesh_terms: D000596:Amino Acids / Q000235:genetics*; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D000818:Animals; D018388:Codon, Terminator / Q000235:genetics; D005815:Genetic Code / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000235:genetics*; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D012343:RNA, Transfer / Q000235:genetics*
chemical_list: D000596:Amino Acids; D018388:Codon, Terminator; D049452:Green Fluorescent Proteins; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine