title: Induced systemic resistance and symbiotic performance of peanut plants challenged with fungal pathogens and co-inoculated with the biocontrol agent Bacillus sp. CHEP5 and Bradyrhizobium sp. SEMIA6144.
abstract: Synergism between beneficial rhizobacteria and fungal pathogens is poorly understood. Therefore, evaluation of co-inoculation of bacteria that promote plant growth by different mechanisms in pathogen challenged plants would contribute to increase the knowledge about how plants manage interactions with different microorganisms. The goals of this work were a) to elucidate, in greenhouse experiments, the effect of co-inoculation of peanut with Bradyrhizobium sp. SEMIA6144 and the biocontrol agent Bacillus sp. CHEP5 on growth and symbiotic performance of Sclerotium rolfsii challenged plants, and b) to evaluate field performance of these bacteria in co-inoculated peanut plants. The capacity of Bacillus sp. CHEP5 to induce systemic resistance against S. rolfsii was not affected by the inoculation of Bradyrhizobium sp. SEMIA6144. This microsymbiont, protected peanut plants from the S. rolfsii detrimental effect, reducing the stem wilt incidence. However, disease incidence in plants inoculated with the isogenic mutant Bradyrhizobium sp. SEMIA6144 V2 (unable to produce Nod factors) was as high as in pathogen challenged plants. Therefore, Bradyrhizobium sp. SEMIA6144 Nod factors play a role in the systemic resistance against S. rolfsii. Bacillus sp. CHEP5 enhanced Bradyrhizobium sp. SEMIA6144 root surface colonization and improved its symbiotic behavior, even in S. rolfsii challenged plants. Results of field trials confirmed the Bacillus sp. CHEP5 ability to protect against fungal pathogens and to improve the yield of extra-large peanut seeds from 2.15% (in Río Cuarto) to 16.69% (in Las Vertientes), indicating that co-inoculation of beneficial rhizobacteria could be a useful strategy for the peanut production under sustainable agriculture system.
authors.affiliation: Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-quimicas y Naturales, Argentina.
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Figueredo
authors.affiliation: Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-quimicas y Naturales, Argentina.
authors.forename: María Laura
authors.initials: ML
authors.lastname: Tonelli
authors.affiliation: Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-quimicas y Naturales, Argentina.
authors.forename: Fernando
authors.initials: F
authors.lastname: Ibáñez
authors.affiliation: Departamento de Producción Vegetal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Agencia Postal 3, 5800 Río Cuarto, Córdoba, Argentina.
authors.forename: Federico
authors.initials: F
authors.lastname: Morla
authors.affiliation: Departamento de Producción Vegetal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Agencia Postal 3, 5800 Río Cuarto, Córdoba, Argentina.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Cerioni
authors.affiliation: Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-quimicas y Naturales, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: Del Carmen Tordable
authors.affiliation: Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-quimicas y Naturales, Argentina. Electronic address: afabra@exa.unrc.edu.ar.
authors.forename: Adriana
authors.initials: A
authors.lastname: Fabra
mesh_terms: D010367:Arachis / Q000254:growth & development / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D001203:Ascomycota / Q000472:pathogenicity / Q000502:physiology; D001407:Bacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development; D061046:Biological Control Agents; D020369:Bradyrhizobium / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D060467:Disease Resistance; D005658:Fungi / Q000472:pathogenicity* / Q000502:physiology; D006790:Host-Parasite Interactions; D007113:Immunity, Innate; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D012988:Soil Microbiology; D013559:Symbiosis
chemical_list: D061046:Biological Control Agents
keywords: Co-inoculation; Induced systemic resistance; Nod factors; Nodulation; Peanut