title: Evaluation of fibrin-gelatin hydrogel as biopaper for application in skin bioprinting: An in-vitro study.
abstract: BACKGROUND
  Recent advances in tissue engineering have led to the development of the concept of bioprinting as an interesting alternative to traditional tissue engineering approaches. Biopaper, a biomimetic hydrogel, is an essential component of the bioprinting process.
  
  
  OBJECTIVE
  The aim of this work was to synthesize a biopaper made of fibrin-gelatin hybrid hydrogel for application in skin bioprinting.
  
  
  METHODS
  Different composition percentages of the two biopolymer hydrogels, fibrin-gelatin, have been studied for the construction of the biopaper and were examined in terms of water absorption, biodegradability, glucose absorption, mechanical properties and water vapor transmission. Subsequently, tissue fusion study was performed on prepared 3T3 fibroblast cell line pellets embedded into the hydrogel.
  
  
  RESULTS
  Based on the obtained results, fibrin-gelatin blend hydrogel with the same proportion of two components provides a natural scaffold for fibroblast-based bioink embedding and culture.
  
  
  CONCLUSIONS
  The suggested optimized hydrogel was a suitable candidate as a biopaper for skin bioprinting technology.
authors.affiliation: Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Mohammad Sadjad
authors.initials: MS
authors.lastname: Hakam
authors.affiliation: Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Rana
authors.initials: R
authors.lastname: Imani
authors.affiliation: Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Nabiollah
authors.initials: N
authors.lastname: Abolfathi
authors.affiliation: Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Fakhrzadeh
authors.affiliation: Department of Pharmacology, Iran University of Medical Science, Tehran, Iran.|Cellular and Molecular Research Center, Iran University of Medical Science, Tehran, Iran.|Tissue Engineering Group (TEG) & Research, National Orthopedic Centre of Excellence in Research & Learning (NOCERAL), Department of Orthopedics, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Sharifi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D062028:Bioprinting; D002470:Cell Survival; D005337:Fibrin / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D012867:Skin; D013917:Thrombin / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D020100:Hydrogels; D005780:Gelatin; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin
keywords: Bioprinting; bioink; biopaper; fibrin; gelatin; skin; tissue fusion