title: Successive membrane separation processes simplify concentration of lipases produced by Aspergillus niger by solid-state fermentation.
abstract: In this study, we developed a simplified method for producing, separating, and concentrating lipases derived from solid-state fermentation of agro-industrial residues by filamentous fungi. First, we used Aspergillus niger to produce lipases with hydrolytic activity. We analyzed the separation and concentration of enzymes using membrane separation processes. The sequential use of microfiltration and ultrafiltration processes made it possible to obtain concentrates with enzymatic activities much higher than those in the initial extract. The permeate flux was higher than 60 L/m2h during microfiltration using 20- and 0.45-µm membranes and during ultrafiltration using 100- and 50-kDa membranes, where fouling was reversible during the filtration steps, thereby indicating that the fouling may be removed by cleaning processes. These results demonstrate the feasibility of lipase production using A. niger by solid-state fermentation of agro-industrial residues, followed by successive tangential filtration with membranes, which simplify the separation and concentration steps that are typically required in downstream processes.
authors.affiliation: Programa de Pós-Graduação em Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, Caixa Postal 743, CEP 99709-910, Erechim, RS, Brazil. reinehr@upf.br.
authors.forename: Christian Oliveira
authors.initials: CO
authors.lastname: Reinehr
authors.affiliation: Programa de Pós-Graduação em Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, Caixa Postal 743, CEP 99709-910, Erechim, RS, Brazil.
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.affiliation: Programa de Pós-Graduação em Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, Caixa Postal 743, CEP 99709-910, Erechim, RS, Brazil.
authors.forename: Marcus Vinicius
authors.initials: MV
authors.lastname: Tres
authors.affiliation: Programa de Pós-Graduação em Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, Caixa Postal 743, CEP 99709-910, Erechim, RS, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Steffens
authors.affiliation: Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos, Universidade de Passo Fundo, Caixa Postal 611, CEP 99052-900, Passo Fundo, RS, Brazil.
authors.forename: Vandré Barbosa
authors.initials: VB
authors.lastname: Brião
authors.affiliation: Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos, Universidade de Passo Fundo, Caixa Postal 611, CEP 99052-900, Passo Fundo, RS, Brazil.
authors.forename: Luciane Maria
authors.initials: LM
authors.lastname: Colla
mesh_terms: D001234:Aspergillus niger; D005285:Fermentation; D005374:Filtration; D008049:Lipase; D014462:Ultrafiltration
chemical_list: D008049:Lipase
keywords: Agro-industrial residues; Aspergillus niger; Lipase; Microfiltration; Ultrafiltration