title: First report of Tasmanian sheep strain (G2) genotype isolated from Iranian goat using the high resolution melting (HRM) analysis.
abstract: OBJECTIVE
  The present study was aimed to evaluateE. granulosusgenotypes isolated from goats using HRM analysis in Isfahan province.
  
  
  BACKGROUND
  Cystic echincoccosis, so-called hydatidosis, is widespread infection caused by the larval stage ofEchinococcus granulosus. This is an important zoonotic disease worldwide, especially in the developing countries such as Iran. To date, molecular studies mainly based on the mitochondrial DNA sequences have identified distinct genotypes termed G1-G10 which can differ in some characteristics such as the growth and infectivity to different intermediate hosts or the survival rate in the definitive hosts that are important for the development of control strategies.
  
  
  METHODS
  From August to December 2014, 1341 goats were investigated and hydatid cysts were collected from the liver and lungs of 43 infected goats in Isfahan province abattoirs, Isfahan, Iran. Total genomic DNA was extracted from each sample, amplified for the presence of polymorphism of mitochondrial gene coding for cytochrome c oxidase subunit 1 (CO1), using high resolution melting curve (HRM) method.
  
  
  RESULTS
  the results of HRM analysis using the sequence of CO1 gene for 43Echinococcus granulosusisolates from goats showed 31, 2 and 10 isolates were identified as G1, G2, and G3 genotypes, respectively.
  
  
  CONCLUSIONS
  G1 is the predominant genotype in the isolated goat samples in Isfahan province, and the presence of G2 strain was reported for the first time in goat in Iran.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran university of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Hosseini-Safa
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Mohag Hegh
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Pestechian
authors.affiliation: Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ganji
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
authors.forename: Rasoul
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mahmoudi Lamouki
authors.affiliation: Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rostami-Nejad
keywords: Echinococcus granulosus; G2 genotype; HRM; Iran; goat