title: CSFV proliferation is associated with GBF1 and Rab2.
abstract: The Golgi apparatus and its resident proteins are utilized and regulated by viruses to facilitate their proliferation. In this study, we investigated Classical swine fever virus (CSFV) proliferation when the function of the Golgi was disturbed. Golgi function was disturbed using chemical inhibitors, namely, brefeldin A (BFA) and golgicide A (GCA), and RNA interfering targets, such as the Golgi-specific BFA-resistance guanine nucleotide exchange factor 1 (GBF1) and Rab2 GTPases. CSFV proliferation was significantly inhibited during RNA replication and viral particle generation after BFA and GCA treatment. CSFV multiplication dynamics were retarded in cells transfected with GBF1 and Rab2 shRNA. Furthermore, CSFV proliferation was promoted by GBF1 and Rab2 overexpression using a lentiviral system. Hence, Golgi function is important for CSFV multiplication, and GBF1 and Rab2 participate in CSFV proliferation. Further studies must investigate Golgi-resident proteins to elucidate the mechanism underlying CSFV replication.
authors.affiliation: College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Wulong
authors.initials: W
authors.lastname: Liang
authors.forename: Minping
authors.initials: M
authors.lastname: Zheng
authors.forename: Changlei
authors.initials: C
authors.lastname: Bao
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020126:Brefeldin A / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D006692:Classical Swine Fever Virus / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D006056:Golgi Apparatus / Q000187:drug effects / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics*; D006801:Humans; D011725:Pyridines / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D012313:RNA / Q000235:genetics*; D034622:RNA Interference; D013552:Swine / Q000235:genetics / Q000821:virology; D020693:rab2 GTP-Binding Protein / Q000235:genetics*
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D011725:Pyridines; D011804:Quinolines; C539165:golgicide A; D020126:Brefeldin A; D012313:RNA; D020693:rab2 GTP-Binding Protein