title: [Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation].
abstract: Objective:To study the genic and non-genic regulation of 50 Hz 0.6 mT pulsed electromagnenic fields (PEMF) on rat calvarial osteoblasts (ROB) differentiation.Methods:ROBs were achieved by enzyme digestion, and treated with 50 Hz 0.6 mT PEMFs for 1.5 hours after subculture. The alkaline phosphatase (ALP) activity, mRNA transcription of ALP, Runx2 and OSX and protein expression of Runx2 and OSX were detected at 0, 3, 6, 9 and 12 hours after PEMF treatment.Results:The ALP activity at 3 hours after treatment was significantly higher than that in the control(P<0.01), while the mRNA transcription of ALP began to increase at 6 hours after treatment. The mRNA transcription of Runx2 increased immediately after treatment and regressed at 6 hours, then increased again. The protein expression of it corresponded but with a little lag. The mRNA transcription of OSX also raised instantly after treatment, then returned to the level of control at 6 hours, and lower than control at 12 hours significantly. The protein expression of it also corresponded but with a bit delay.Conclusions:There are genic regulation for the protein expression of Runx2 and OSX, and non-genic regulation for the ALP activity on the process of 50 Hz 0.6 mT PEMFs prompts ROBs differentiation.
authors.affiliation: Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Fang
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Wengui
authors.initials: W
authors.lastname: Shi
authors.affiliation: Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Juanli
authors.initials: J
authors.lastname: Yan
authors.affiliation: Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China. chenkm@lut.cn.
authors.forename: Keming
authors.initials: K
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000528:radiation effects*; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism / Q000528:radiation effects; D004574:Electromagnetic Fields; D010006:Osteoblasts / Q000737:chemistry / Q000528:radiation effects*; D010012:Osteogenesis / Q000235:genetics* / Q000528:radiation effects*; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism / Q000528:radiation effects
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493717:Runx2 protein, rat; C495181:Sp7 protein, rat; D014157:Transcription Factors; D000469:Alkaline Phosphatase