title: Development of an ex vivo human-porcine respiratory model for preclinical studies.
abstract: Anatomical models to study aerosol delivery impose huge limitations and extrapolation to humans remains controversial. This study aimed to develop and validate an ex vivo human-like respiratory tract model easy to use and relevant to compare to in vivo human data. A human plastinated head is connected to an ex vivo porcine pulmonary tract ventilated artificially by passive expansion. A physiological study measures "pleural" depressions, tidal volumes, and minute ventilation for the respiratory rates chosen (10, 15, and 20 per minute) with three inspiratory/expiratory ratios (1/1, 1/2, and 1/3). Scintigraphy with81mKrypton assesses the homogeneity of the ventilation. Forty different experiments were set for validation, with 36 (90%) ventilating successfully. At a respiratory rate of 15/minute with inspiratory/expiratory ratio of 1/2, the tidal volume average was 824 mL (standard deviation, 207 mL). The scintigraphy performed on 16 ex vivo models (44.4%), showed homogenous ventilation with great similarity to human physiological studies. Ratio of the peripheral to central count rates were equally correlated with human data published in the literature. This new model, combining research feasibility and human physiology likeness, provides a realistic approach to human inhalation and therefore can be an interesting tool in aerosol regional deposition studies.
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Perinel
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Pourchez
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France.
authors.forename: Lara
authors.initials: L
authors.lastname: Leclerc
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: John
authors.initials: J
authors.lastname: Avet
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|Centre Hospitalier Emile Roux, F-43012, Le Puy en Velay, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Durand
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Prévôt
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Cottier
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Jean M
authors.initials: JM
authors.lastname: Vergnon
mesh_terms: D000818:Animals; D023421:Models, Animal; D011877:Radionuclide Imaging; D012119:Respiration; D012137:Respiratory System; D013552:Swine / Q000502:physiology*; D013990:Tidal Volume