title: Manuka Honey Exerts Antioxidant and Anti-Inflammatory Activities That Promote Healing of Acetic Acid-Induced Gastric Ulcer in Rats.
abstract: Gastric ulcers are a major problem worldwide with no effective treatment. The objective of this study was to evaluate the use of manuka honey in the treatment of acetic acid-induced chronic gastric ulcers in rats. Different groups of rats were treated with three different concentrations of honey. Stomachs were checked macroscopically for ulcerative lesions in the glandular mucosa and microscopically for histopathological alterations. Treatment with manuka honey significantly reduced the ulcer index and maintained the glycoprotein content. It also reduced the mucosal myeloperoxidase activity, lipid peroxidation (MDA), and the inflammatory cytokines (TNF-α, IL-1β, and IL-6) as compared to untreated control group. In addition, honey-treated groups showed significant increase in enzymatic (GPx and SOD) and nonenzymatic (GSH) antioxidants besides levels of the anti-inflammatory cytokine IL-10. Flow cytometry studies showed that treatment of animals with manuka honey has normalized cell cycle distribution and significantly lowered apoptosis in gastric mucosa. In conclusion, the results indicated that manuka honey is effective in the treatment of chronic ulcer and preservation of mucosal glycoproteins. Its effects are due to its antioxidant and anti-inflammatory properties that resulted in a significant reduction of the gastric mucosal MDA, TNF-α, IL-1β, and IL-6 and caused an elevation in IL-10 levels.
authors.affiliation: Biology Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Saad B
authors.initials: SB
authors.lastname: Almasaudi
authors.affiliation: Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Biotechnology Research Laboratories, Gastroenterology Surgery Center, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Aymn T
authors.identifier: 0000-0001-7372-370X
authors.initials: AT
authors.lastname: Abbas
authors.affiliation: Biology Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Rashad R
authors.initials: RR
authors.lastname: Al-Hindi
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta 31111, Egypt.
authors.forename: Nagla A
authors.identifier: 0000-0003-2593-6879
authors.initials: NA
authors.lastname: El-Shitany
authors.affiliation: Animal Facility Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Umama A
authors.initials: UA
authors.lastname: Abdel-Dayem
authors.affiliation: Anatomy Department (Cytology and Histology), Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Soad S
authors.initials: SS
authors.lastname: Ali
authors.affiliation: Department of Animal Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Rasha M
authors.initials: RM
authors.lastname: Saleh
authors.affiliation: Department of Hematology and Yousef Abdulatif Jameel Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Soad K
authors.initials: SK
authors.lastname: Al Jaouni
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Yousef Abdulatif Jameel Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Mohammad Amjad
authors.identifier: 0000-0003-0088-0565
authors.initials: MA
authors.lastname: Kamal
authors.affiliation: Special Infectious Agents Unit, King Fahd Medical Research Center, Yousef Abdullatif Jameel Chair of Prophetic Medicine Application, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Steve M
authors.identifier: 0000-0001-7512-8787
authors.initials: SM
authors.lastname: Harakeh