title: Could mucin 16 and colony-stimulating factor 2-receptor beta possible graft versus host disease biomarkers? Medical hypotheses.
abstract: Graft versus host disease (GVHD) occurs after bone marrow transplantation and is one of the most important causes of death worldwide. Reviews demonstrated GVHD patients with involvement of various tissues and organs, such as salivary glands. The diagnosis of acute GVHD has been the biopsies and the histopathologic evaluation of tissue from an involved organ. These procedures are invasive. Saliva proteins as possible biomarker for GVHD could facilitate the management and diagnosis accuracy. For support the proposed hypotheses, in this pilot study we collected whole saliva samples from patients with undergoing allogeneic hematopoietic cell transplantation (HCT) and from healthy subjects. Samples were collected prospectively between pre-transplant, thirty days, one hundred and, two hundred days after transplant. The proteomic profile was analyzed using SDS-PAGE and LCMS-ESI-IT-TOF mass spectrometry. The relevant personal data, past medical history were also recorded. The most relevant proteins found exclusively in GVHD patients were: CSF2RB, Protocadherin (Pcdh) Fat 2 precursor, protein capicua homolog isoform CIC-S, MUC16 and RGPD8_HUMAN RANBP2. This study aims to conduct an initial evaluation of the possible presence of such biomarkers in saliva from GVHD patients, and suggested a potential application of proteomics analysis as a alternative method to diagnose GVHD.
authors.affiliation: Department of Dermatology, School of Medicine, University of São Paulo, 255, Dr. Enéas de Carvalho Aguiar Avenue, 3° Floor, 05403-900, Cerqueira Cesar, São Paulo, Brazil. Electronic address: milenamsouza@usp.br.
authors.forename: Milena Monteiro de
authors.initials: MM
authors.lastname: Souza
authors.affiliation: Departament of Immunology of Schistosomiasis, Hospital das Clínicas, School of Medicine, University of São Paulo, Brazil. Electronic address: fabiana.paula@hc.fm.usp.br.
authors.forename: Fabiana Martins
authors.initials: FM
authors.lastname: de Paula
authors.affiliation: Departament of General Pathology, Dental School, University of São Paulo, 2227, Prof. Lineu Prestes Avenue, 05508-000, Cidade Universitária, São Paulo, Brazil. Electronic address: r.hsieh@usp.br.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Hsieh
authors.affiliation: Department of Blone Marrow Transplantation, Brazilian Institute of Cancer Control, 2576, Alcântara Machado Avenue, 03102-000, Brás, São Paulo, Brazil. Electronic address: mariacristina@biosanas.com.br.
authors.forename: Maria Cristina Martins Almeida
authors.initials: MC
authors.lastname: Macedo
authors.affiliation: Departament of Infectious and Parasitic Diseases, School of Medicine, University of São Paulo, 255, Dr. Enéas de Carvalho Aguiar Avenue, 05403-000, Cerqueira Cesar, São Paulo, Brazil. Electronic address: marcelo.corral@usp.br.
authors.forename: Marcelo Andreetta
authors.initials: MA
authors.lastname: Corral
authors.affiliation: Departament of Diagnostic Oral, Dental School, University of São Paulo, 2227, Prof. Lineu Prestes Avenue, 05508-000, Cidade Universitária, São Paulo, Brazil. Electronic address: thaborg@gmail.com.
authors.forename: Thaís Borguezan
authors.initials: TB
authors.lastname: Nunes
authors.affiliation: Departament of Diagnostic Oral, Dental School, University of São Paulo, 2227, Prof. Lineu Prestes Avenue, 05508-000, Cidade Universitária, São Paulo, Brazil. Electronic address: f.paula@usp.br.
authors.forename: Fernanda
authors.initials: F
authors.lastname: De Paula
authors.affiliation: Departament of General Pathology, Dental School, University of São Paulo, 2227, Prof. Lineu Prestes Avenue, 05508-000, Cidade Universitária, São Paulo, Brazil. Electronic address: silvialourenco@usp.br.
authors.forename: Silvia Vanessa
authors.initials: SV
authors.lastname: Lourenço
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D018394:CA-125 Antigen / Q000032:analysis*; D053648:Cytokine Receptor Common beta Subunit / Q000032:analysis*; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis*; D064368:Healthy Volunteers; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000628:therapy; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008962:Models, Theoretical; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy; D040901:Proteomics; D012463:Saliva / Q000737:chemistry
chemical_list: D015415:Biomarkers; D018394:CA-125 Antigen; C115876:CSF2RB protein, human; D053648:Cytokine Receptor Common beta Subunit; C451759:MUC16 protein, human; D008565:Membrane Proteins