title: A paper-based multiplexed resonance energy transfer nucleic acid hybridization assay using a single form of upconversion nanoparticle as donor and three quantum dots as acceptors.
abstract: Monodisperse aqueous upconverting nanoparticles (UCNPs) were covalently immobilized on aldehyde modified cellulose paper via reductive amination to evaluate the multiplexing capacity of luminescence resonance energy transfer (LRET) between UCNPs and quantum dots (QDs). This is the first account of a multiplexed bioassay strategy that demonstrates the principle of use of a single form of UCNP as donor and three different color emitting QDs as acceptors to concurrently determine three analytes. Broad absorbance profiles of green, orange and red QDs that spanned from the first exciton absorption peak to the UV region were in overlap with a blue emission band from UCNPs composed of NaYF4that was doped with 30% Yb3+, 0.5% Tm3+, allowing for LRET that was stimulated using 980 nm near-infrared radiation. The characteristic narrow and well-defined emission peaks of UCNPs and QDs allowed for the collection of luminescence from each nanoparticle using a band-pass optical filter and an epi-fluorescence microscope. The LRET system was used for the concurrent detection of uidA, Stx1A and tetA gene fragments with selectivity even in serum samples, and reached limits of detection of 26 fmol, 56 fmol and 76 fmol, respectively.
authors.affiliation: Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada.
authors.forename: Samer
authors.initials: S
authors.lastname: Doughan
authors.affiliation: Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada.
authors.forename: Uvaraj
authors.initials: U
authors.lastname: Uddayasankar
authors.affiliation: Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada.
authors.forename: Aparna
authors.initials: A
authors.lastname: Peri
authors.affiliation: Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada. Electronic address: ulrich.krull@utoronto.ca.
authors.forename: Ulrich J
authors.initials: UJ
authors.lastname: Krull
mesh_terms: D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008024:Ligands; D010209:Paper; D045663:Quantum Dots / Q000737:chemistry*; D022621:Shiga Toxin / Q000032:analysis; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D008024:Ligands; D022621:Shiga Toxin; D013752:Tetracycline
keywords: Bioassay; Luminescence resonance energy transfer; Multiplexed detection; Paper; Quantum dot; Upconversion nanoparticle