title: Inhibition of calcineurin by FK506 stimulates germinal vesicle breakdown of mouse oocytes in hypoxanthine-supplemented medium.
abstract: Calcineurin (CN) is a serine/threonine phosphatase which plays important roles in meiosis maturation in invertebrate oocytes; however, the role of CN in mouse oocytes is relatively unexplored. In this study, we examined the expression, localization and functional roles of CN in mouse oocytes and granulosa cells. The RT-PCR results showed that theβisoform of calcineurin A subunit (Cn A) expressed significantly higher thanαandγisoforms, and the expression of Cn AβmRNA obviously decreased in oocytes in which germinal vesicle breakdown (GVBD) occurred, while only B1 of calcineurin B subunit (Cn B) was detected in oocytes and stably expressed during oocytes maturation. The following fluorescence experiment showed that Cn A was mainly located in the nucleus of germinal vesicle (GV) stage oocytes and gruanlosa cells, and subsequently dispersed into the entire cytoplasm after GVBD. The decline of Cn A in oocytes suggested that it may play an important role in GVBD. To further clarify the role of calcineurin during meiotic maturation, FK506 (a calcineurin inhibitor) was used in the culture medium contained hypoxanthine (HX) which could keep mouse oocytes staying at GV stage. As expected, FK506 could induce a significant elevation of GVBD rate and increase the MPF level of denuded oocytes (DOs). Furthermore, FK506 could also play an induction role of GVBD of oocytes in COCs and follicles, and the process could be counteracted by MAPK kinase inhibitor (U0126). Above all, the results implied that calcineurin might play a crucial role in development of mouse oocytes and MPF and MAPK pathways are involved in this process.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, HuaZhong Agriculture University , Wu Han , Hu Bei Province , People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, HuaZhong Agriculture University, Wu Han, Hu Bei Province, People's Republic of China; Department of Animal Husbandry and Veterinary, Wuhan Agricultural School, Wuhan, Hu Bei Province, People's Republic of China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zhen
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, HuaZhong Agriculture University, Wu Han, Hu Bei Province, People's Republic of China; Reproductive Medicine Center, Second Affiliated Hospital of Wenzhou Medical College, Wen Zhou, People's Republic of China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, HuaZhong Agriculture University , Wu Han , Hu Bei Province , People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, HuaZhong Agriculture University , Wu Han , Hu Bei Province , People's Republic of China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, HuaZhong Agriculture University , Wu Han , Hu Bei Province , People's Republic of China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Huo
keywords: Calcineurin; FK506; GVBD; MAPK; MPF