title: Hemorrhagic metalloproteinase, Cc HSM-III, isolated from Cerastes cerastes venom: Purification and biochemical characterization.
abstract: Snake venom metalloproteinases are the most abundant toxins in Viperidae venoms. In this study, a new hemorrhagin, Cc HSM-III (66 kDa), was purified from Cerastes cerastes venom by gel filtration, ion exchange, and reversed-phase high-performance liquid chromatographies. The analysis of Cc HSM-III by liquid chromatography with a tandem mass spectrometry revealed 32 peptides sharing a homology with P-III metalloproteinases from Echis ocellatus snake venom. Cc HSM-III displays hemorrhagic activity with a minimal hemorrhagic dose of 5 μg, which is abolished by ethylene diamine tetracetic acid but not by phenylmethylsulfonyl fluoride. The mechanism underlying Cc HSM-III hemorrhagic activity is probably due to its extensive proteolytic activity against type IV collagen. Cc HSM-III induces local tissue damage and an inflammatory response by upregulating both matrix metalloproteinase 2 and 9 in skin of mice. Thus, Cc HSM-III may play a key role in the pathogenesis of C. cerastes envenomation.
authors.affiliation: USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia Bab Ezzouar, 16111, Algiers, Algeria.
authors.forename: Wafa
authors.initials: W
authors.lastname: Tachoua
authors.affiliation: USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia Bab Ezzouar, 16111, Algiers, Algeria.
authors.forename: Hinda
authors.initials: H
authors.lastname: Boukhalfa-Abib
authors.affiliation: USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia Bab Ezzouar, 16111, Algiers, Algeria.
authors.forename: Fatima
authors.initials: F
authors.lastname: Laraba-Djebari
mesh_terms: D000818:Animals; D006470:Hemorrhage / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D045726:Metalloproteases / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D051379:Mice; D012867:Skin / Q000378:metabolism / Q000473:pathology; D014757:Viper Venoms / Q000737:chemistry*; D017819:Viperidae
chemical_list: D014757:Viper Venoms; D045726:Metalloproteases
keywords: Cerastes cerastes; de novo sequencing; hemorrhage; metalloproteinase