title: In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5' termini of ccmB transcripts.
abstract: In plant mitochondria, the 5' ends of many transcripts are generated post-transcriptionally. We show that the pentatricopeptide repeat (PPR) protein RNA PROCESSING FACTOR 4 (RPF4) supports the generation of extra 5' ends of ccmB transcripts in Landsberg erecta (Ler) and a number of other Arabidopsis thaliana ecotypes. RPF4 was identified in Ler applying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which generates the Ler-type extra ccmB 5' termini only after the introduction of the RPF4 allele from Ler. Studies with chimeric RPF4 proteins composed of various parts of the RPF4 proteins from Ler and Col identified differences in the N-terminal and central PPR motifs that explain ecotype-specific variations in ccmB processing. These results fit well with binding site predictions in ccmB transcripts based on the known determinants of nucleotide base recognition by PPR motifs.
authors.affiliation: Institut Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Stoll
authors.affiliation: Institut Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Jonietz
authors.affiliation: Institut Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Schleicher
authors.affiliation: Australian Research Council 40 Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, 6009, Australia.
authors.forename: Catherine Colas
authors.initials: CC
authors.lastname: des Francs-Small
authors.affiliation: Australian Research Council 40 Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, 6009, Australia.
authors.forename: Ian
authors.initials: I
authors.lastname: Small
authors.affiliation: Institut Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany. stefan.binder@uni-ulm.de.
authors.forename: Stefan
authors.identifier: http://orcid.org/0000-0003-0037-2640
authors.initials: S
authors.lastname: Binder
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D060146:Ecotype; D018506:Gene Expression Regulation, Plant; D024101:Mitochondrial Proteins / Q000235:genetics; D012313:RNA; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D018749:RNA, Plant / Q000235:genetics; D020449:Repetitive Sequences, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D024101:Mitochondrial Proteins; C000593376:RFL9 protein, Arabidopsis; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D018749:RNA, Plant; D012313:RNA
keywords: 5′ end polymorphisms; Arabidopsis thaliana; Mitochondria; Pentatricopeptide repeat proteins; RNA processing