title: Diosmetin, a Potential p53 Activator, Performs Anticancer Effect by Regulating Cell Cycling and Cell Proliferation in HepG2 Cells.
abstract: Diosmetin (Dios), a flavone aglycone, is a major active ingredient of many herbal medicines. Previous studies implicated that the potential antitumor activities of Dios are mediated through multiple cell signaling pathways, however more investigations are required to reveal the targets and mechanisms of Dios. In this study, our results demonstrated that cell cycle progression and proliferation of HepG2 cells were inhibited by Dios. Meanwhile, Dios-induced liver cancer cell apoptosis was related with the p53 activation. After PFT-α, a p53 inhibitor was added into Dios-treated HepG2 cells, cell growth inhibition was partially reversed. These findings defined and supported that p53 is a novel target of Dios. By activating p53, Dios exerts anticancer effects by inducing cell cycle arrest and apoptosis in HepG2 cells.
authors.affiliation: Infection department of Internal Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, Guangdong Province. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Clinical Research Center. China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Infection Department of Internal Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001. China.
authors.forename: Shuna
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Xuyi People's Hospital, Xuyi 211700. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ying
authors.affiliation: Clinical Research Center.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632. China.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kurihara
authors.affiliation: Infection Department of Internal Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001. China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Zheng
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, Guangdong Province. China.
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: He
authors.affiliation: Laboratory of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical University, Guangdong 524001. China.
authors.forename: Runzhi
authors.initials: R
authors.lastname: Zhu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D005419:Flavonoids; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C039602:diosmetin
keywords: Diosmetin; ERK and JNK phosphorylation; HepG2; P53; apoptosis; flavonoids