title: Programmed Self-Assembly of Hierarchical Nanostructures through Protein-Nanoparticle Coengineering.
abstract: Hierarchical organization of macromolecules through self-assembly is a prominent feature in biological systems. Synthetic fabrication of such structures provides materials with emergent functions. Here, we report the fabrication of self-assembled superstructures through coengineering of recombinant proteins and nanoparticles. These structures feature a highly sophisticated level of multilayered hierarchical organization of the components: individual proteins and nanoparticles coassemble to form discrete assemblies that collapse to form granules, which then further self-organize to generate superstructures with sizes of hundreds of nanometers. The components within these superstructures are dynamic and spatially reorganize in response to environmental influences. The precise control over the molecular organization of building blocks imparted by this protein-nanoparticle coengineering strategy provides a method for creating hierarchical hybrid materials.
authors.affiliation: Department of Chemistry, University of Massachusetts , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Rubul
authors.identifier: 0000-0001-6125-5873
authors.initials: R
authors.lastname: Mout
authors.affiliation: Department of Chemistry, University of Massachusetts , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Gulen
authors.initials: G
authors.lastname: Yesilbag Tonga
authors.affiliation: Department of Chemistry, University of Massachusetts , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Massachusetts , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Moumita
authors.initials: M
authors.lastname: Ray
authors.affiliation: Department of Chemistry, University of Massachusetts , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Trinava
authors.initials: T
authors.lastname: Roy
authors.affiliation: Department of Chemistry, University of Massachusetts , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Vincent M
authors.identifier: 0000-0002-5184-5439
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
keywords: biomimetic self-assembly; gold nanoparticle; hierarchical assembly; molecular organization; protein engineering