title: Effect of α-linolenic acid-rich diacylglycerol oil on protein kinase C activation in the rat digestive tract and lingual mucosa.
abstract: 1,2-Diacylglycerol with short chain fatty acids is an endogenous activator of protein kinase C (PKC), which involved in multiple cellular processes implicated in cancer. The aim of the present study was to assess the effects of dietary α-linolenic acid-rich diacylglycerol (ALA-DAG) oil on PKC activation in the rat digestive tract and lingual mucosa in comparison with the effects of α-linolenic acid-rich triacylglycerol (ALA-TAG) oil, and common dietary oil. Membranous PKC activity in the lingual mucosa of male Wistar rats was significantly activated by treatment of the tongue with 1,2-tetradecarnoylphorbol-13-acetate (100 μM) twice in 1 day. In contrast, animals consuming a diet containing either ALA-DAG oil (7.5% or 30%), ALA-TAG oil (7.5% or 30%), or rapeseed oil (30%) for 4 weeks exhibited no significant differences in the cytosolic and membrane PKC activity in the lingual, esophageal, gastric, small intestinal, and colonic mucosa. Dose-related increases in PKC activity were not observed in the ALA-DAG oil-fed groups. Thus, the effects of dietary ALA-DAG oil on PKC activation in the digestive tract and lingual mucosa was similar to those of the ALA-TAG and rapeseed oils. These findings suggest that replacement of common dietary oil with ALA-DAG oil would not increase the risk of carcinogenesis.
authors.affiliation: Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan. Electronic address: mori.takuya@kao.co.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Mori
authors.affiliation: Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Honda
authors.affiliation: Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Ishii
authors.affiliation: Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Koga
authors.affiliation: Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Okahara
authors.affiliation: Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Onishi
authors.affiliation: Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Kawamoto
authors.affiliation: Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shimotoyodome
mesh_terms: D000818:Animals; D004032:Diet; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D041981:Gastrointestinal Tract / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects* / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D017208:Rats, Wistar; D014280:Triglycerides / Q000494:pharmacology; D017962:alpha-Linolenic Acid / Q000494:pharmacology*
chemical_list: C011439:1,2-diacylglycerol; D004042:Dietary Fats, Unsaturated; D004075:Diglycerides; D010938:Plant Oils; D014280:Triglycerides; D017962:alpha-Linolenic Acid; D011493:Protein Kinase C
keywords: Alpha-linolenic acid; Diacylglycerol; Digestive tract mucosa; Protein kinase C; Triacylglycerol