title: Identification and Characterization of Enzymes Catalyzing Pyrazolopyrimidine Formation in the Biosynthesis of Formycin A.
abstract: Genome scanning of Streptomyces kaniharaensis, the producer of formycin A, reveals two sets of purA, purB, purC, and purH genes. The Pur enzymes catalyze pyrimidine assembly of purine nucleobases. To test whether enzymes encoded by the second set of pur genes catalyze analogous transformations in formycin biosynthesis, formycin B 5'-phosphate was synthesized and shown to be converted by ForA and ForB to formycin A 5'-phosphate. These results support that For enzymes are responsible for formycin formation.
authors.affiliation: Department of Chemistry, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Yeonjin
authors.initials: Y
authors.lastname: Ko
authors.affiliation: Department of Chemistry, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Shao-An
authors.initials: SA
authors.lastname: Wang
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ogasawara
authors.affiliation: Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Ruszczycky
authors.affiliation: Department of Chemistry, University of Texas at Austin , Austin, Texas 78712, United States.|Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas at Austin , Austin, Texas 78712, United States.
authors.forename: Hung-Wen
authors.identifier: 0000-0001-8953-4794
authors.initials: HW
authors.lastname: Liu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D053898:Biosynthetic Pathways; D005573:Formycins / Q000096:biosynthesis*; D016680:Genome, Bacterial; D006801:Humans; D015394:Molecular Structure; D005810:Multigene Family; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D011720:Pyrazoles / Q000378:metabolism*; D011743:Pyrimidines / Q000096:biosynthesis*; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D005573:Formycins; D011720:Pyrazoles; D011743:Pyrimidines; C034550:formycin; D009699:N-Glycosyl Hydrolases; C030681:purine nucleosidase