title: Allograft inflammatory factor 1 is a regulator of transcytosis in M cells.
abstract: M cells in follicle-associated epithelium (FAE) are specialized antigen-sampling cells that take up intestinal luminal antigens. Transcription factor Spi-B regulates M-cell maturation, but the molecules that promote transcytosis within M cells are not fully identified. Here we show that mouse allograft inflammatory factor 1 (Aif1) is expressed by M cells and contributes to M-cell transcytosis. FAE in Aif1-/-mice has suppressed uptake of particles and commensal bacteria, compared with wild-type mice. Translocation of Yersinia enterocolitica, but not of Salmonella enterica serovar Typhimurium, leading to the generation of antigen-specific IgA antibodies, is also diminished in Aif1-deficient mice. Although β1 integrin, which acts as a receptor for Y. enterocolitica via invasin protein, is expressed on the apical surface membranes of M cells, its active form is rarely found in Aif1-/-mice. These findings show that Aif1 is important for bacterial and particle transcytosis in M cells.
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.|Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Sari
authors.initials: S
authors.lastname: Kishikawa
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.|International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.|Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Tokyo 102-0076, Japan.|Mucosal Vaccine Project, BIKEN Innovative Vaccine Research Alliance Laboratories, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.|Mucosal Vaccine Project, BIKEN Center for Innovative Vaccine Research and Development, The Research Foundation for Microbial Diseases of Osaka University, Osaka 565-0871, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Sato
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneto
authors.affiliation: Department of Neurochemistry, National Institute of Neuroscience, Tokyo 187-8502, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Uchino
authors.affiliation: Department of Neurochemistry, National Institute of Neuroscience, Tokyo 187-8502, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kohsaka
authors.affiliation: Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Division of Mucosal Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.|International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.|Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Tokyo 102-0076, Japan.|Department of Immunology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D018988:Bacterial Translocation; D002135:Calcium-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D002452:Cell Count; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape; D020895:Enterocytes / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D007778:Lactobacillus / Q000502:physiology; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000172:deficiency / Q000378:metabolism*; D057900:Transcytosis; D015007:Yersinia / Q000502:physiology
chemical_list: C491049:Aif1 protein, mouse; D000941:Antigens; D002135:Calcium-Binding Proteins; D007070:Immunoglobulin A; D019012:Integrin beta1; D008840:Microfilament Proteins