title: Effect of dexmedetomidine on acute lung injury in experimental ischemia-reperfusion model.
abstract: OBJECTIVE
  Ischemia-reperfusion injury is one of the consequences of tourniquet application for extremity surgery. The aim of the study was to establish the effect of dexmedetomidine on the acute lung injury following lower extremity experimental ischemia-reperfusion model in rats.
  
  
  METHODS
  Twenty-eight Wistar-Albino breed Rats were recruited after Ethics Committee approval and allocated into 4 groups, each with 7 subjects. Group 1 (SHAM) received only anesthesia. Group 2 (IR) had experienced 3h of ischemia and 3h of reperfusion using left lower extremity tourniquet after anesthesia application. Groups 3 (D-50) and 4 (D-100) had undergone the same procedures as in the Group 2, except for receiving 50 and 100mg·kg-1, respectively, dexmedetomidine intraperitoneally 1h before the tourniquet release. Blood samples were obtained for the analysis of tumor necrosing factor-α and interleukin-6. Pulmonary tissue samples were obtained for histological analysis.
  
  
  RESULTS
  No significant difference regarding blood tumor necrosing factor-α and interleukin-6 values was found among the groups, whereas pulmonary tissue injury scores revealed significant difference. Histological scores obtained from the Group 2 were significantly higher from those in the Groups 1, 3 and 4 with p-values 0.001 for each comparison. Moreover, Group 1 scores were found to be significantly lower than those in the Groups 3 and 4 with p-values 0.001 and 0.011, respectively. No significant difference was observed between the Groups 3 and 4.
  
  
  CONCLUSIONS
  Dexmedetomidine is effective in reduction of the experimental ischemia-reperfusion induced pulmonary tissue injury in rats, formed by extremity tourniquet application.
authors.affiliation: Adıyaman University Research and Educational Hospital, Department of Anesthesiology and Reanimation, Adıyaman, Turkey.
authors.forename: Ömer Burak
authors.initials: ÖB
authors.lastname: Küçükebe
authors.affiliation: Dokuz Eylül University Medical Faculty, Department of Anesthesiology and Reanimation, İzmir, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Özzeybek
authors.affiliation: Adıyaman University Research and Educational Hospital, Department of Anesthesiology and Reanimation, Adıyaman, Turkey. Electronic address: ruslan_jnr@hotmail.com.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Abdullayev
authors.affiliation: Zonguldak Atatürk Hospital, Department of Anesthesiology and Reanimation, Zonguldak, Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Ustaoğlu
authors.affiliation: Dokuz Eylül University Medical Faculty, Department of Histology and Embryology, İzmir, Turkey.
authors.forename: Işıl
authors.initials: I
authors.lastname: Tekmen
authors.affiliation: Dokuz Eylül University Medical Faculty, Department of Medical Biochemistry, İzmir, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Küme
mesh_terms: D055371:Acute Lung Injury / Q000517:prevention & control*; D058647:Adrenergic alpha-2 Receptor Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D020927:Dexmedetomidine / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D015850:Interleukin-6 / Q000097:blood; D035002:Lower Extremity / Q000098:blood supply; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy*; D014111:Tourniquets / Q000009:adverse effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D020927:Dexmedetomidine
keywords: Acute lung injury; Dexmedetomidina; Dexmedetomidine; Experimental; Lesão de reperfusão; Lesão pulmonar aguda; Reperfusion injury