title: Extended depth-of-field microscopy with a high-speed deformable mirror.
abstract: We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.
authors.forename: William J
authors.initials: WJ
authors.lastname: Shain
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Vickers
authors.forename: Bennett B
authors.initials: BB
authors.lastname: Goldberg
authors.forename: Thomas
authors.initials: T
authors.lastname: Bifano
authors.forename: Jerome
authors.initials: J
authors.lastname: Mertz