title: Anaerobic Dehalogenation of Chloroanilines by Dehalococcoides mccartyi Strain CBDB1 and Dehalobacter Strain 14DCB1 via Different Pathways as Related to Molecular Electronic Structure.
abstract: Dehalococcoides mccartyi strain CBDB1 and Dehalobacter strain 14DCB1 are organohalide-respiring microbes of the phyla Chloroflexi and Firmicutes, respectively. Here, we report the transformation of chloroanilines by these two bacterial strains via dissimilar dehalogenation pathways and discuss the underlying mechanism with quantum chemically calculated net atomic charges of the substrate Cl, H, and C atoms. Strain CBDB1 preferentially removed Cl doubly flanked by two Cl or by one Cl and NH2, whereas strain 14DCB1 preferentially dechlorinated Cl that has an ortho H. For the CBDB1-mediated dechlorination, comparative analysis with Hirshfeld charges shows that the least-negative Cl discriminates active from nonactive substrates in 14 out of 15 cases and may represent the preferred site of primary attack through cob(I)alamin. For the latter trend, three of seven active substrates provide strong evidence, with partial support from three of the remaining four substrates. Regarding strain 14DCB1, the most positive carbon-attached H atom discriminates active from nonactive chloroanilines in again 14 out of 15 cases. Here, regioselectivity is governed for 10 of the 11 active substrates by the most positive H attached to the highest-charge (most positive or least negative) aromatic C carrying the Cl to be removed. These findings suggest the aromatic ring H as primary site of attack through the supernucleophile Co(I), converting an initial H bond to a full electron transfer as start of the reductive dehalogenation. For both mechanisms, one- and two-electron transfer to Cl (strain CBDB1) or H (strain 14DCB1) are compatible with the presently available data. Computational chemistry research into reaction intermediates and pathways may further aid in understanding the bacterial reductive dehalogenation at the molecular level.
authors.affiliation: Institute for Organic Chemistry, Technical University Bergakademie Freiberg , Leipziger Straße 29, 09596 Freiberg, Germany.
authors.forename: Shangwei
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute for Organic Chemistry, Technical University Bergakademie Freiberg , Leipziger Straße 29, 09596 Freiberg, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Wondrousch
authors.forename: Myriel
authors.initials: M
authors.lastname: Cooper
authors.affiliation: Department of Microbiology, Cornell University , Ithaca, New York 14853, United States.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Zinder
authors.affiliation: Institute for Organic Chemistry, Technical University Bergakademie Freiberg , Leipziger Straße 29, 09596 Freiberg, Germany.
authors.forename: Gerrit
authors.identifier: 0000-0002-3789-1703
authors.initials: G
authors.lastname: Schüürmann
authors.affiliation: Fachgebiet Applied Biochemistry, Technische Universität Berlin , Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
authors.forename: Lorenz
authors.identifier: 0000-0001-8205-0842
authors.initials: L
authors.lastname: Adrian
mesh_terms: D041862:Chloroflexi / Q000378:metabolism*; D054879:Halogenation; D015394:Molecular Structure; D010459:Peptococcaceae