title: Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant.
abstract: Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and biomedicine. Overexpression of soluble and active mTG in E. coli has been limited due to improper protein folding and requirement for proteolytic cleavage of the pro-domain. Furthermore, to integrate mTG more fully industrially and academically, thermostable and solvent-stable variants may be imperative.
  
  
  
  A novel expression system constitutively producing active mTG was designed. Wild-type (WT) mTG and a S2P variant had similar expression levels, comparable to previous studies. Kinetic constants were determined by a glutamate dehydrogenase-coupled assay, and the S2P variant showed an increased affinity and a doubled enzyme efficiency towards Z-Gln-Gly. The melting temperature (Tm) of the WT was determined by intrinsic fluorescence measurements to be 55.8 ± 0.1 °C and of the S2P variant to be 56.3 ± 0.4 °C and 45.5 ± 0.1 °C, showing a moderately different thermostability profile. Stability in water miscible organic solvents was determined for both the WT and S2P variant. Of the solvents tested, incubation of mTG in isopropanol for 24 h at 4 °C showed the strongest stabilizing effect with mTG retaining 61 and 72% activity for WT and S2P respectively in 70% isopropanol. Both enzymes also showed an increased initial activity in the presence of organic solvents with the highest activity increase in 40% DMSO. Nevertheless, both enzymes were inactivated in 70% of all organic solvents tested.
  
  
  
  A constitutive expression system of active mTG in E. coli without downstream proteolytic cleavage processing was used for overexpression and characterization. High throughput techniques for testing thermostability and kinetics were useful in streamlining analysis and could be used in the future for quickly identifying beneficial mutants. Hitherto untested thermostability and stability of mTG in organic solvents was evaluated, which can pave the way for use of the enzyme in novel applications and processes.
authors.affiliation: Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.
authors.forename: Gabe
authors.initials: G
authors.lastname: Javitt
authors.affiliation: Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.
authors.forename: Zohar
authors.initials: Z
authors.lastname: Ben-Barak-Zelas
authors.affiliation: NanoTemper Technologies GmbH Flößergasse 4, 81369, Munich, Germany.
authors.forename: Moran
authors.initials: M
authors.lastname: Jerabek-Willemsen
authors.affiliation: Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel. afishman@tx.technion.ac.il.
authors.forename: Ayelet
authors.initials: A
authors.lastname: Fishman
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D014644:Genetic Variation; D015202:Protein Engineering / Q000379:methods*; D013045:Species Specificity; D013379:Substrate Specificity; D011503:Transglutaminases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D011503:Transglutaminases
keywords: Differential scanning fluorimeter; Microbial transglutaminase; Organic solvents; Thermostable