title: Production of Whole-Cell Lipase from Streptomyces clavuligerus in a Bench-Scale Bioreactor and Its First Evaluation as Biocatalyst for Synthesis in Organic Medium.
abstract: This work evaluated a wild-type Streptomyces clavuligerus strain as a whole-cell lipase (Sc-WCL) producer by submerged fermentation. In an orbital shaker, lipase hydrolytic activity of 3000 U L-1, measured at pH 9.0 and 37 °C by using p-nitrophenyl palmitate as substrate, was achieved after 36 h fermentation using glycerol-free production medium in a baffled Erlenmeyer flask at 28 °C and pH 6.8. Maximum productivity of 52.5 U L-1h-1was achieved after 24 h in bioreactor using glycerol-free production medium at pH 6.8 and 28 °C, with agitation at 400 rpm and aeration at 1 vvm. Sc-WCL was shown to be more active at 60 °C and pH 10.7, while higher activity retention was observed at 30-40 °C after 1 h incubation at pH 10. Sc-WCL showed to have potential to be used as biocatalyst in hydrolysis and esterification reactions. In the hydrolysis of p-nitrophenyl palmitate, lyophilized Sc-WCL expressed a hydrolytic activity (330 units g-1solid, measured at 37 °C and pH 9.0) around 100-fold higher than the ones declared by a supplier of lyophilized powders of mixtures of intracellular lipases from Thermus thermophiles and Thermus flavus (≥3.0 units g-1solid, measured at 65 °C and pH 8.0). In the synthesis of butyl butyrate in anhydrous medium, 85% ester conversion was achieved at 37 °C after 8 h reaction. Thus, Sc-WCL showed to be a promising biocatalyst because it is cheaper than the isolated and purified lipases.
authors.affiliation: Programa de Pós-Graduação em Engenharia Química, Universidade Federal de São Carlos, Rod. Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil.
authors.forename: Jéssica Bravin Carmello
authors.initials: JBC
authors.lastname: Dos Santos
authors.affiliation: Departamento de Engenharia Química, Universidade Federal de São Carlos, Rod. Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil.
authors.forename: Rosineide Gomes
authors.initials: RG
authors.lastname: da Silva Cruz
authors.affiliation: Programa de Pós-Graduação em Engenharia Química, Universidade Federal de São Carlos, Rod. Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil. pwtardioli@ufscar.br.|Departamento de Engenharia Química, Universidade Federal de São Carlos, Rod. Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil. pwtardioli@ufscar.br.
authors.forename: Paulo Waldir
authors.initials: PW
authors.lastname: Tardioli
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry*; D019149:Bioreactors; D002384:Catalysis; D003470:Culture Media / Q000737:chemistry*; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D008049:Lipase
keywords: Biochemical properties; Butyl butyrate; Esterification activity; Hydrolytic activity; Streptomyces clavuligerus; Submerged cultivation; Whole-cell lipase