title: Isolation, Characterization, and Expansion of Cancer Stem Cells.
abstract: The ability to isolate, characterize, and expand distinct tumor cell populations from primary tissue or xenografts is vital to identifying molecular mechanisms specific to cancer stem cells. Once cells have been extracted from tissue, there are multiple methods by which they can be sorted and cultured. We will describe the approaches that can be taken from cancer stem cell isolation through expansion, including Magnetic-activated Cell Sorting (MACS), Fluorescence-activated Cell Sorting (FACS), the use of reporter systems, and various cell culture methods.
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Ave., NC10, Cleveland, OH, 44915, USA.
authors.forename: Luke A
authors.initials: LA
authors.lastname: Torre-Healy
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Ave., NC10, Cleveland, OH, 44915, USA.
authors.forename: Artem
authors.initials: A
authors.lastname: Berezovsky
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Ave., NC10, Cleveland, OH, 44915, USA. lathiaj@ccf.org.|Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, 44195, USA. lathiaj@ccf.org.|Case Comprehensive Cancer Center, Cleveland, OH, 44106, USA. lathiaj@ccf.org.
authors.forename: Justin D
authors.initials: JD
authors.lastname: Lathia
mesh_terms: D015415:Biomarkers; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D058948:Cell Tracking / Q000379:methods; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype; D014410:Tumor Stem Cell Assay
chemical_list: D015415:Biomarkers
keywords: Cancer stem cell; Cell culture; FACS; MACS; Reporter system; Sorting; Xenograft