title: Construction of a library of structurally diverse ribonucleopeptides with catalytic groups.
abstract: Functional screening of structurally diverse libraries consisting of proteins or nucleic acids is an effective method to obtain receptors or aptamers with unique molecular recognition characteristics. However, further modification of these selected receptors to exert a newly desired function is still a challenging task. We have constructed a library of structurally diverse ribonucleopeptides (RNPs) that are modified with a catalytic group, in which the catalytic group aligns with various orientations against the ATP binding pocket of RNA subunit. As a proof-of-principle, the screening of the constructed RNP library for the catalytic reaction of ester hydrolysis was successfully carried out. The size of both the substrate-binding RNA library and the catalytic group modified peptide library are independently expandable, and thus, the size of RNPs library could be enlarged by a combination of these two subunits. We anticipate that the library of functionalized and structurally diverse RNPs would be expanded for various other catalytic reactions.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Nakano
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Nakata
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: t-morii@iae.kyoto-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D000595:Amino Acid Sequence; D001483:Base Sequence; D020134:Catalytic Domain; D010455:Peptides / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D010455:Peptides; D012261:Ribonucleoproteins; D000255:Adenosine Triphosphate
keywords: Catalytic activity; Receptor; Ribonucleopeptide; Structurally diverse library