title: MicroRNA expression profiling in canine prostate cancer.
abstract: Canine prostate cancer (cPCa) is an untreatable malignant neoplasm resulting in local tissue invasion and distant metastasis. MicroRNAs (miRs) are small non-coding RNAs that function as oncogenes or tumor suppressors. The purpose of this study was to characterize the expression of miRs that are altered in cPCa tissue. The expression levels of 277 mature miRs in prostatic tissue (n=5, respectively) were compared between the non-tumor and tumor groups using real-time PCR. Five miRs (miR-18a, 95, 221, 222 and 330) were up-regulated, but 14 miRs (miR-127, 148a, 205, 299, 329b, 335, 376a, 376c, 379, 380, 381, 411, 487b and 495) were down-regulated specifically in cPCa (P<0.05). These miRs have potential use as early diagnosis markers for cPCa and in miR-based therapy.
authors.affiliation: Laboratory of Reproduction, Division of Therapeutic Science II, Department of Clinical Veterinary Medicine, School of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Akiko
authors.initials: A
authors.lastname: Saito
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Masaki
authors.initials: M
authors.lastname: Michishita
authors.forename: Masato
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Mami
authors.initials: M
authors.lastname: Irimajiri
authors.forename: Takeharu
authors.initials: T
authors.lastname: Kaneda
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ochiai
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
authors.forename: Kimimasa
authors.initials: K
authors.lastname: Takahashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000662:veterinary*; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D035683:MicroRNAs; D012334:RNA, Neoplasm