title: Arabidopsis Regenerating Protoplast: A Powerful Model System for Combining the Proteomics of Cell Wall Proteins and the Visualization of Cell Wall Dynamics.
abstract: The development of a range of sub-proteomic approaches to the plant cell wall has identified many of the cell wall proteins. However, it remains difficult to elucidate the precise biological role of each protein and the cell wall dynamics driven by their actions. The plant protoplast provides an excellent means not only for characterizing cell wall proteins, but also for visualizing the dynamics of cell wall regeneration, during which cell wall proteins are secreted. It therefore offers a unique opportunity to investigate the de novo construction process of the cell wall. This review deals with sub-proteomic approaches to the plant cell wall through the use of protoplasts, a methodology that will provide the basis for further exploration of cell wall proteins and cell wall dynamics.
authors.affiliation: Laboratory of Plant Cell Wall Biology, Graduate School of Life Sciences, Tohoku University, Sendai 890-8578, Japan. ryokoyama@m.tohoku.ac.jp.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Yokoyama
authors.affiliation: Laboratory of Plant Cell Wall Biology, Graduate School of Life Sciences, Tohoku University, Sendai 890-8578, Japan. kuki@biology.tohoku.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kuki
authors.affiliation: Laboratory of Plant Cell Wall Biology, Graduate School of Life Sciences, Tohoku University, Sendai 890-8578, Japan. tkuroha@m.tohoku.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kuroha
authors.affiliation: Laboratory of Plant Cell Wall Biology, Graduate School of Life Sciences, Tohoku University, Sendai 890-8578, Japan. nishitan@m.tohoku.ac.jp.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nishitani
keywords: cell wall; imaging technique; protoplast; sub-proteomics