title: The role of hydrogen sulfide in homocysteine-induced cardiodynamic effects and oxidative stress markers in the isolated rat heart.
abstract: This study aimed to assess the role of H2S in homocysteine-induced cardiodynamic effects in the isolated rat heart. The hearts were retrogradely perfused according to the Langendorff technique. The maximum and minimum rates of pressure in the left ventricle (dp/dt max, dp/dt min), systolic and diastolic left ventricular pressures (SLVP, DLVP), heart rate (HR), and coronary flow (CF) were measured. A spectrophotometrical method was used to measure the following oxidative stress markers: index of lipid peroxidation (thiobarbituric acid reactive substances, TBARS), nitrite level (NO2-), superoxide anion radicals (O2•-), and hydrogen peroxide (H2O2) concentrations. The administration of 10 µmol/l DL-homocysteine (DL-Hcy) alone decreased dp/dt max, SLVP, and CF but did not change any oxidative stress parameters. The administration of 10 µmol/l DL-propargylglycine (DL-PAG) decreased all cardiodynamic parameters and increased the concentration of O2•-. The co-administration of DL-Hcy and DL-PAG induced a significant decrease in all estimated cardiodynamic parameters and decreased the concentration of NO2-and O2•-but increased the levels of TBARS and H2O2. Homocysteine shows a lower pro-oxidative effect in the presence of hydrogen sulfide (H2S), which indicates a potential anti-oxidative capacity of H2S.
authors.affiliation: 1 Department of Physiology, Faculty of Medical Sciences, University of Kragujevac , Kragujevac, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Stojanovic
authors.affiliation: 1 Department of Physiology, Faculty of Medical Sciences, University of Kragujevac , Kragujevac, Serbia.
authors.forename: V
authors.initials: V
authors.lastname: Zivkovic
authors.affiliation: 1 Department of Physiology, Faculty of Medical Sciences, University of Kragujevac , Kragujevac, Serbia.
authors.forename: I
authors.initials: I
authors.lastname: Srejovic
authors.affiliation: 1 Department of Physiology, Faculty of Medical Sciences, University of Kragujevac , Kragujevac, Serbia.
authors.forename: V
authors.initials: V
authors.lastname: Jakovljevic
authors.affiliation: 2 Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac , Kragujevac, Serbia.
authors.forename: N
authors.initials: N
authors.lastname: Jeremic
authors.affiliation: 3 Institute of Medical Physiology "Richard Burian", School of Medicine, University of Belgrade , Belgrade, Serbia.
authors.forename: D
authors.initials: D
authors.lastname: Djuric
mesh_terms: D000480:Alkynes / Q000494:pharmacology; D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D003542:Cystathionine gamma-Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D064426:Gasotransmitters; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006321:Heart / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D006711:Homocystine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D000067268:Isolated Heart Preparation; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010477:Perfusion; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D016277:Ventricular Function, Left / Q000187:drug effects*; D017725:Ventricular Pressure / Q000187:drug effects*
chemical_list: D000480:Alkynes; D004791:Enzyme Inhibitors; D064426:Gasotransmitters; D009573:Nitrites; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D006711:Homocystine; C009055:propargylglycine; D006861:Hydrogen Peroxide; D003542:Cystathionine gamma-Lyase; D005998:Glycine; D006862:Hydrogen Sulfide
keywords: H2S; Langendorff technique; cardiodynamics; homocysteine; oxidative stress