title: Intravital multiphoton imaging of mouse tibialis anterior muscle.
abstract: Intravital imaging by multiphoton microscopy is a powerful tool to gain invaluable insight into tissue biology and function. Here, we provide a step-by-step tissue preparation protocol for imaging the mouse tibialis anterior skeletal muscle. Additionally, we include steps for jugular vein catheterization that allow for well-controlled intravenous reagent delivery. Preparation of the tibialis anterior muscle is minimally invasive, reducing the chances of inducing damage and inflammation prior to imaging. The tibialis anterior muscle is useful for imaging leukocyte interaction with vascular endothelium, and to understand muscle contraction biology. Importantly, this model can be easily adapted to study neuromuscular diseases and myopathies.
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Lau
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
authors.forename: Chi Ching
authors.initials: CC
authors.lastname: Goh
authors.affiliation: Department of Microbiology and Immunology, The Peter Doherty Institute, The University of Melbourne , Parkville, Victoria, Australia.
authors.forename: Sapna
authors.initials: S
authors.lastname: Devi
authors.affiliation: Department of Medicine, University of Melbourne, Department of Rheumatology, Royal Melbourne Hospital, Inflammation Division, Walter and Eliza Hall Institute of Medical Research , Melbourne, Australia.
authors.forename: Jo
authors.initials: J
authors.lastname: Keeble
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
authors.forename: Peter
authors.initials: P
authors.lastname: See
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
authors.forename: Florent
authors.initials: F
authors.lastname: Ginhoux
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
authors.forename: Lai Guan
authors.initials: LG
authors.lastname: Ng
keywords: fluorescent; inflammation; multiphoton imaging; skeletal muscle; vascular