title: Flow cytometric analysis as an additional predictive tool of treatment response in children with chronic-phase chronic myeloid leukemia treated with imatinib.
abstract: Bone marrow samples of newly diagnosed children with chronic-phase chronic myeloid leukemia (CML) were obtained at diagnosis and after imatinib initiation and stained with anti-human CD34, CD38, CD123, CD45RA, cMpl, and lineage antibodies. Flow cytometric analysis revealed that granulocyte macrophage progenitor predominance in CML progenitors at diagnosis and elevated cMpl expression in bone marrow progenitors at 3 months may predict poor outcome in children with chronic-phase CML treated with imatinib. We recommend flow cytometric analysis of bone marrow in the early phase of treatment, as it is a convenient tool that may predict treatment response and guide CML management.
authors.affiliation: Department of Pediatrics, Keio University School of Medicine, Tokyo, Japan.|Clinical Research Center, National Hospital Organization Nagoya Medical Center, Aichi, Japan.
authors.forename: Haruko
authors.initials: H
authors.lastname: Shima
authors.affiliation: Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kiyokawa
authors.affiliation: Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Miharu
authors.affiliation: Department of Pediatrics, University of Fukui Faculty of Medical Sciences, Fukui, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tanizawa
authors.affiliation: Department of Pediatrics, Dokkyo Medical University, Tochigi, Japan.
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Kurosawa
authors.affiliation: Department of Pediatrics, Niigata Cancer Center Hospital, Niigata, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Watanabe
authors.affiliation: Department of Pediatrics, Soma General Hospital, Fukushima, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ito
authors.affiliation: Department of Pediatrics, Iwate Prefectural Chubu Hospital, Aomori, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Tono
authors.affiliation: Department of Hematology/Oncology, Tokyo Metropolitan Children's Medical Center, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yuza
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Aichi, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Muramatsu
authors.affiliation: Department of Pediatrics, Japan Community Healthcare Organization Tokuyama Central Hospital, Yamaguchi, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Hotta
authors.affiliation: Department of Pediatrics, Nagasaki University School of Medicine, Nagasaki, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Okada
authors.affiliation: Department of Pediatrics, Hiroshima Red Cross Hospital & Atomic-Bomb Survivors Hospital, Hiroshima, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Hamamoto
authors.affiliation: Department of Pediatrics, Yokohama City University School of Medicine, Kanagawa, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kajiwara
authors.affiliation: Clinical Research Center, National Hospital Organization Nagoya Medical Center, Aichi, Japan.
authors.forename: Akiko M
authors.initials: AM
authors.lastname: Saito
authors.affiliation: Clinical Research Center, National Hospital Organization Nagoya Medical Center, Aichi, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Horibe
authors.affiliation: Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Shuki
authors.initials: S
authors.lastname: Mizutani
authors.affiliation: Human Health Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Souichi
authors.initials: S
authors.lastname: Adachi
authors.affiliation: Department of Pediatrics, Ehime University Graduate School of Medicine, Ehime, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Ishii
authors.affiliation: Department of Pediatrics, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimada
mesh_terms: D000293:Adolescent; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001854:Bone Marrow Cells / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D000068877:Imatinib Mesylate / Q000627:therapeutic use*; D015466:Leukemia, Myeloid, Chronic-Phase / Q000188:drug therapy*; D008297:Male; D014411:Neoplastic Stem Cells / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D000068877:Imatinib Mesylate
keywords: cMpl; chronic myeloid leukemia; flow cytometry