title: [MicroRNA-20a Promotes Osteogenic Differentiation of C3H/10T1/2 Cells through Regulating CKIP-1 Expression].
abstract: OBJECTIVE
  To investigate the effect of microRNA-20a(MiR-20a) on osteogenic differentiation of mouse C3H/10T1/2 cells and its regulatory mechanism.
  
  
  METHODS
  Osteogenic differentiation of C3H/10T1/2 cells were identified by ALP staining and qRT-PCR. MiR-20a mimics and CKIP-1 siRNA were transfected into C3H/10T1/2 cells respectively with lipo3000. The expression of osteoblast marker genes, miR-20a and CKIP-1 were quantitatively assessed by qRT-PCR.
  
  
  RESULTS
  miR-20a expression was up-regulated during osteoblast differentiation of C3H/10T1/2 cells. Overexpression of miR-20a promoted osteogenic differentiation. Furthermore, miR-20a inhibited the expression of bone formation negative regulator CKIP-1. Additionally, CKIP-1 knockdown promoted osteogenic differentiation.
  
  
  CONCLUSIONS
  MiR-20a promotes osteogenic differentiation of C3H/10T1/2 cells possibly through inhibiting the expression of CKIP-1.
authors.affiliation: Department of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Jun-Fen
authors.initials: JF
authors.lastname: Chen
authors.affiliation: Department of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Yang
authors.affiliation: Department of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Xi-Xi
authors.initials: XX
authors.lastname: Dong
authors.affiliation: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Dong
authors.affiliation: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Huang
authors.affiliation: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: smilovjiang@163.corn.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Jiang
authors.affiliation: Department of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China. E-mail: caojk@hotmail.com.
authors.forename: Jun-Kai
authors.initials: JK
authors.lastname: Cao
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002454:Cell Differentiation; D055785:Gene Knockdown Techniques; D051379:Mice; D008809:Mice, Inbred C3H; D035683:MicroRNAs / Q000502:physiology*; D010006:Osteoblasts; D010012:Osteogenesis; D047908:Intracellular Signaling Peptides and Proteins
chemical_list: D002352:Carrier Proteins; D035683:MicroRNAs; C000620556:PLEKHO1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins