title: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay.
abstract: A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. The dataset includes expression values fordrpt,il11a,il1b1,il1b2,il1b3,il1r-like-1(e3-5),il1r-like-1(e9-11),il1r1-like-a,il1r1-like-b,il1r2,saa,tnfa1,tnfa2,tnfa3,tnfrsf1a,tnfrsf1a-like-a,tnfrsf1a-like-b,tnfrsf5, andtnfrsf9. Gene expression was measured at four time-points post-challenge in both a resistant line (ARS-Fp-R) and a susceptible line (ARS-Fp-S) of rainbow trout. In addition, fish body weight, spleen index and theFlavobacterium psychrophilumload are reported. These data are an extension of information presented and discussed in"Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge withFlavobacterium psychrophilumin resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)" (Kutyrev et al., 2016) [1].
authors.affiliation: National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430, USA; Institute of General and Experimental Biology, Siberian Brunch of Russian Academy of Sciences, Sakhyanovoi St., 6, 670047 Ulan-Ude, Russia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kutyrev
authors.affiliation: National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430, USA.
authors.forename: Beth
authors.initials: B
authors.lastname: Cleveland
authors.affiliation: National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Leeds
authors.affiliation: National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430, USA.
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Wiens
keywords: Bacterial cold water disease; Cytokine; Disease resistance; Flavobacterium psychrophilum; Rainbow trout