title: Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology.
abstract: Many cell types undergo migration during embryogenesis and disease. Endothelial cells line blood vessels and lymphatics, which migrate during development as part of angiogenesis, lymphangiogenesis and other types of vessel remodelling. These processes are also important in wound healing, cancer metastasis and cardiovascular conditions. However, the molecular control of endothelial cell migration is poorly understood. Here, we present a dataset containing siRNA screens that identify known and novel components of signalling pathways regulating migration of lymphatic endothelial cells. These components are compared to signalling in blood vascular endothelial cells. Further, using high-content microscopy, we captured a dataset of images of migrating cells following transfection with a genome-wide siRNA library. These datasets are suitable for the identification and analysis of genes involved in endothelial cell migration and morphology, and for computational approaches to identify signalling networks controlling the migratory response and integration of cell morphology, gene function and cell signaling. This may facilitate identification of protein targets for therapeutically modulating angiogenesis and lymphangiogenesis in the context of human disease.
authors.affiliation: Tumour Angiogenesis Program, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia.|Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia.
authors.forename: Steven P
authors.identifier: 0000-0002-9382-4337
authors.initials: SP
authors.lastname: Williams
authors.affiliation: Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia.
authors.forename: Cathryn M
authors.initials: CM
authors.lastname: Gould
authors.affiliation: Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia.|The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
authors.forename: Cameron J
authors.initials: CJ
authors.lastname: Nowell
authors.affiliation: Tumour Angiogenesis Program, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia.|Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Tara
authors.initials: T
authors.lastname: Karnezis
authors.affiliation: Tumour Angiogenesis Program, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia.|Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Marc G
authors.initials: MG
authors.lastname: Achen
authors.affiliation: Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia.|Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Kaylene J
authors.initials: KJ
authors.lastname: Simpson
authors.affiliation: Tumour Angiogenesis Program, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia.|Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Stacker
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D042783:Endothelial Cells; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D034741:RNA, Small Interfering