title: miR-200bc/429 Inhibits Osteosarcoma Cell Proliferation and Invasion by Targeting PMP22.
abstract: BACKGROUND MicroRNAs (miRNAs) are small non-coding RNAs which play a crucial role in diverse biological processes and could contribute to cancer development and progression. MiR-200bc/429 have been found to be aberrantly expressed in osteosarcoma (OS). However, the features of miR-200bc/429 in the tumorigenesis and progress of OS remain poorly understood. MATERIAL AND METHODS The miR-200bc/429 expression was firstly identified in human OS clinical samples and cell lines by quantitative real-time PCR (qRT-PCR). After transfection with miR-200bc/429 mimics or negative control in U2OS or MG63 cells, cell proliferation was measured by CCK-8 assay. Following that, wound-healing assay and Transwell invasion assay were performed to evaluate cell migration and invasion, respectively. Finally, luciferase reporter assay and Western blot analysis were performed to determine if peripheral myelin protein-22 (PMP22) is a direct target of miR-200bc/429. RESULTS Results revealed that miR-200bc/429 were significantly depressed in human OS tissues and cell lines by qRT-PCR. Then, restoration of miR-200bc/429 significantly inhibited cell proliferation (P<0.05) and invasion (P<0.05) in vitro. Luciferase reporter assay and Western blot analysis revealed that miR-200bc/429 could directly target PMP22 3' untranslated region (UTR) and inhibit its expression in U2OS and MG63 cells. CONCLUSIONS These findings suggest that miR-200bc/429 inhibit OS cells proliferation and invasion by targeting PMP22, and function as a tumor suppressor and may be a patent molecular marker as well as a potential target for OS therapy.
authors.affiliation: Department of Orthopedics, Tianjin third central hospital, Tianjin, China (mainland).
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Tianjin third central hospital, Tianjin, China (mainland).
authors.forename: Han
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Tianjin third central hospital, Tianjin, China (mainland).
authors.forename: Lianping
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Orthopedics, Tianjin third central hospital, Tianjin, China (mainland).
authors.forename: Shusen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Tianjin third central hospital, Tianjin, China (mainland).
authors.forename: Jinxin
authors.initials: J
authors.lastname: Zheng
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000502:physiology; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D020413:3' Untranslated Regions; C533896:MIRN200 microRNA, human; C546363:MIRN429 microRNA, human; D035683:MicroRNAs; D009185:Myelin Proteins; C073687:PMP22 protein, human