title: Inhibition of bone formation in rats by aluminum exposure via Wnt/β-catenin pathway.
abstract: The previous research found that aluminum trichloride (AlCl3) inhibited rat osteoblastic differentiation through inactivation of Wnt/β-catenin signaling pathway in vitro. On that basis, the experiment in vivo was conducted in this study. Rats were orally exposed to 0 (control group) and 0.4 g/L AlCl3(AlCl3-treated group) for 30, 60, 90 or 120 days, respectively. We found that mRNA expressions of type I collagen and insulin-like growth factor-1, mRNA and protein expressions of Runx2 and survivin, ratio of p-GSK3β/GSK3β and protein expression of β-catenin were all decreased, whereas the mRNA and protein expressions Dkk1 and sFRP1 and the mRNA expressions and activity of Caspase-3 were increased in the AlCl3-treated group compared with the control group with time prolonged. These results suggest that AlCl3inhibits bone formation and induces bone impairment by inhibiting the Wnt/β-catenin signaling pathway in young growing rats.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Wanyue
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Shen
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: liyanfei@neau.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China. Electronic address: zhuyanzhu@caas.cn.
authors.forename: Yanzhu
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002712:Chlorides / Q000633:toxicity*; D024042:Collagen Type I / Q000378:metabolism; D005269:Femur / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D024042:Collagen Type I; C548904:insulin-like growth factor-1, rat; D000077410:Aluminum Chloride; D007334:Insulin-Like Growth Factor I; D053148:Caspase 3
keywords: Aluminum trichloride; Bone formation; Rat; Wnt/β-catenin signaling pathway