title: Efficient utilization of hemicellulose and cellulose in alkali liquor-pretreated corncob for bioethanol production at high solid loading by Spathaspora passalidarum U1-58.
abstract: The bioethanol fermentation of pretreated corncob was investigated using Spathaspora passalidarum U1-58, which simultaneously utilizes glucose and xylose for high-efficiency ethanol production. Two approaches, namely, separate hydrolysis and co-fermentation (SHCF) and simultaneous saccharification and co-fermentation (SSCF), were optimized to test the ethanol fermentation potential of U1-58. The highest ethanol titer of 42.46g/L and yield of 72.12% were acquired in SHCF, whereas 53.24g/L ethanol and yield of 75.35% were obtained in SSCF at solid-to-liquid ratio of 1:5 (w/v). Approximately 86.20% of cellulose and 82.99% of hemicellulose were consumed in SSCF after 96h, and at least 10.49g/L ethanol was produced from hemicellulose, which corresponded to 37.59% of the theoretical yield. Compared with the published cellulosic ethanol fermentation cases, the present work presented high ethanol titer and yield, and cellulose and hemicellulose could be efficiently utilized for ethanol production.
authors.affiliation: School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Guo
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address: yfchen@tust.edu.cn.
authors.forename: Yefu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Gengxin
authors.initials: G
authors.lastname: Fu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Dongguang
authors.initials: D
authors.lastname: Xiao
mesh_terms: D000434:Alcoholic Beverages; D000468:Alkalies / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D004718:Saccharomycetales / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D000468:Alkalies; D011134:Polysaccharides; D000431:Ethanol; C007916:hemicellulose; D002482:Cellulose; D014994:Xylose; D005947:Glucose
keywords: Bioethanol; Co-fermentation of xylose and glucose; Corncob; High solid loading; Spathaspora passalidarum