title: Cordycepin diminishes thymic stromal lymphopoietin-induced interleukin-13 production.
abstract: Atopic dermatitis (AD) is known to aggravate by thymic stromal lymphopoietin (TSLP) and TSLP is also known to up-regulate mast cell proliferation via production of interleukin (IL)-13. Thus, we investigated whether cordycepin could regulate mast cell proliferation induced by TSLP in human mast cell line, HMC-1 cell. Cordycepin significantly diminished the production and mRNA of IL-13 through the down-regulation of phosphorylated-signal transducer and activation of transcription 6 in the TSLP-stimulated HMC-1 cells. Cordycepin also significantly diminished the cell proliferation via down-regulating MDM2 and Bcl2 levels and up-regulating p53, caspase-3, and cleaved poly ADP-ribose polymerase levels in the TSLP-stimulated HMC-1 cells. Moreover, cordycepin significantly diminished the production of IL-6, tumor necrosis factor-α, and IL-1β in the TSLP-stimulated HMC-1 cells. In conclusion, our study shows that cordycepin has potential effect for the treatment of allergic inflammatory diseases through the blockade of IL-13 and MDM2 exacerbated by TSLP.
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
authors.forename: Myoung-Schook
authors.initials: MS
authors.lastname: Yoou
authors.affiliation: Department of Biotechnology, Hoseo University, 20, Hoseo-ro 79beon-gil, Baebang-eup, Asan, Chungcheongnam-do 31499, Republic of Korea.
authors.forename: Kyoung Wan
authors.initials: KW
authors.lastname: Yoon
authors.affiliation: Department of Food Science & Technology, Hoseo University, 20, Hoseo-ro 79beon-gil, Baebang-eup, Asan, Chungcheongnam-do 31499, Republic of Korea.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea. Electronic address: hmkim@khu.ac.kr.
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Department of Food Science & Technology, Hoseo University, 20, Hoseo-ro 79beon-gil, Baebang-eup, Asan, Chungcheongnam-do 31499, Republic of Korea. Electronic address: hjjeong@hoseo.edu.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D018926:Anti-Allergic Agents / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D003839:Deoxyadenosines / Q000494:pharmacology*; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism; D050800:STAT6 Transcription Factor / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D018926:Anti-Allergic Agents; C000595707:BCL2 protein, human; D016207:Cytokines; D003839:Deoxyadenosines; D018793:Interleukin-13; D010750:Phosphoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D050800:STAT6 Transcription Factor; D016159:Tumor Suppressor Protein p53; D051736:Proto-Oncogene Proteins c-mdm2; D053148:Caspase 3; C058120:cordycepin; D000094632:Thymic Stromal Lymphopoietin
keywords: Cordycepin; Interleukin-13; Murine double minute 2; Signal transducer and activator of transcription 6; Thymic stromal lymphopoietin