title: New production process of the antifungal chaetoglobosin A using cornstalks.
abstract: Chaetoglobosin A is an antibacterial compound produced by Chaetomium globosum, with potential application as a biopesticide and cancer treatment drug. The aim of this study was to evaluate the feasibility of utilizing cornstalks to produce chaetoglobosin A by C. globosum W7 in solid-batch fermentation and to determine an optimal method for purification of the products. The output of chaetoglobosin A from the cornstalks was 0.34mg/g, and its content in the crude extract was 4.80%. Purification conditions were optimized to increase the content of chaetoglobosin A in the crude extract, including the extract solvent, temperature, and pH value. The optimum process conditions were found to be acetone as the extractant, under room temperature, and at a pH value of 13. Under these conditions, a production process of the antifungal chaetoglobosin A was established, and the content reached 19.17%. Through further verification, cornstalks could replace crops for the production of chaetoglobosin A using this new production process. Moreover, the purified products showed great inhibition against Rhizoctonia solani, with chaetoglobosin A confirmed as the main effective constituent (IC50=3.88μg/mL). Collectively, these results demonstrate the feasibility of using cornstalks to synthesize chaetoglobosin A and that the production process established in this study was effective.
authors.affiliation: Harbin Institute of Technology, School of Life Sciences and Technology, Harbin, Heilongjiang, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Harbin Institute of Technology, School of Life Sciences and Technology, Harbin, Heilongjiang, People's Republic of China.
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Song
authors.affiliation: Harbin Institute of Technology, School of Chemical Engineering and Technology, Harbin, Heilongjiang, People's Republic of China.
authors.forename: Junzheng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Harbin Institute of Technology, School of Life Sciences and Technology, Harbin, Heilongjiang, People's Republic of China. Electronic address: microbio207@gmail.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002145:Callosities / Q000378:metabolism / Q000382:microbiology*; D002597:Chaetomium / Q000378:metabolism*; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology; D014866:Waste Products / Q000032:analysis
chemical_list: D000935:Antifungal Agents; D026121:Indole Alkaloids; D014866:Waste Products; C019290:chaetoglobosins
keywords: Chaetoglobosin A; Chaetomium globosum; Cornstalk; Purification; Stability