title: [Clinical significance of expressions of EVI1 and BRE genes in 47 acute leukemia patients with MLL rearrangement].
abstract: Objective:To investigate the overexpression frequencies of BRE and EVI1, the correlation between BRE and EVI1 expressions and their possible clinical implications in 11q23/MLL rearrangement acute leukemia.Methods:Cytogenetic examination of bone marrow cells was performed by short-term culture method. R-banding technique was used for karyotype analysis. 47 patients were detected by interphase fluorescence in situ hybridization (FISH) with dual-color break apart MLL probe. The expressions of EVI1 and BRE genes were detected by real time quantitative reverse transcription polymerase chain reaction (RQ-PCR) . The correlation and prognostic significance were statistically tested.Results:11q23/MLL rearrangements were confirmed by karyotyping and FISH, respectively in 47 patients. According to immunophenotypic analyses of 37 patients, 5 patients showed positive for CD19, CD79a or CD10, 1 for CD7; the others for CD33, CD13, CD14 and CD15, and 16 of them for CD34. Of the 47 patients, 18 patients showed EVI1 overexpression and most of them presented with t (6;11) and M(4)/M(5). The EVI1 expression was high in t (6;11) or t (9;11) subgroup comparable with levels observed in normal subgroup (P=0.038, 0.022, respectively) . 15 patients showed high BRE expression, and most of them presented with t (9;11) and M(4)/M(5). High BRE expression was found in t (4;11) , t (6;11) , t (9;11) and t (11;19) subgroups, respectively by comparing with normal subgroup. The BRE expression was higher in t (4;11) (P=0.004) or t (9;11) (P=0.012) subgroup than in t (6;11) subgroup. Patients with EVI1 overexpression had a short survival compared with those with low EVI1 expression (P=0.049) and it also did in t (9;11) subgroup (P=0.024) . Patients with t (9;11) and high BRE expression had a long survival compared with those with t (9;11) and low BRE expression (P=0.024) .Conclusion:The EVI1 overexpression was significantly frequent in acute leukemia patients with 11q23/MLL rearranged, especially within t (6;11) subgroup and M(4)/M(5), which was associated with an inferior outcome. High BRE expression was observed frequently in 11q23/MLL-rearranged acute leukemia especially within t (9;11) subgroup and M(5).
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Homeostasis, Suzhou 215006, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Gong
authors.forename: J R
authors.initials: JR
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: S X
authors.initials: SX
authors.lastname: Bai
authors.forename: J N
authors.initials: JN
authors.lastname: Cen
authors.forename: H J
authors.initials: HJ
authors.lastname: Shen
authors.forename: H Y
authors.initials: HY
authors.lastname: Chou
authors.forename: S N
authors.initials: SN
authors.lastname: Chen
authors.forename: J L
authors.initials: JL
authors.lastname: Pan
mesh_terms: D000208:Acute Disease; D001854:Bone Marrow Cells; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11; D015321:Gene Rearrangement; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute; D051788:Myeloid-Lymphoid Leukemia Protein; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C497422:KMT2A protein, human; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase
keywords: Gene rearrangement, MLL; Gene, BRE; Gene, EVI1; In situ hybridization, fluorescence; Leukemia, acute