title: Design of elution strategy for simultaneous detection of chloramphenicol and gentamicin in complex samples using surface plasmon resonance.
abstract: For the analysis of massive samples containing multiple analytes, the enhancement of detection efficiency is crucial. In this study, a facile method was developed for sequential detection of chloramphenicol (CAP) and gentamicin (GEN) in complex samples, e.g. milk, using a surface plasmon resonance (SPR)-based biosensor. Based on the immune inhibition format, two conjugates of antigen and bovine serum albumin (BSA)-denoted as CAP-BSA and GEN-BSA-were grafted on the same channel of the SPR sensor chip. Two standard curves for CAP and GEN were separately obtained by first mixing a single antibody with different concentrations of the relevant antigen. Moreover, different regeneration solutions were screened for sequential analysis. An alkaline solution was found to completely remove the antibody against GEN (AbGEN) from the chip, but it exhibited limited ability to dissociate the antibody against CAP (AbCAP). Therefore, alkaline solution and Gly-HCl solutions are successively applied to elute AbGEN and AbCAP, respectively. By gradual elutions, CAP and GEN concentrations were simultaneously calculated with limit of detection values of 5.28ng/mL and 2.26ng/mL, respectively. Furthermore, the spiking milk samples with CAP and GEN validated the assay with recoveries of 77.6-101.1%. Therefore, this method is expected to improve the detection efficiency of SPR biosensors.
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Yinqiang
authors.initials: Y
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China. Electronic address: surx@tju.edu.cn.
authors.forename: Rongxin
authors.initials: R
authors.lastname: Su
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR China.
authors.forename: Renliang
authors.initials: R
authors.lastname: Huang
authors.affiliation: Research Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: Research Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410001, China. Electronic address: wanglb1@126.com.
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000906:Antibodies / Q000737:chemistry; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005839:Gentamicins / Q000032:analysis*; D055767:Immobilized Proteins / Q000737:chemistry; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D005839:Gentamicins; D055767:Immobilized Proteins; D012710:Serum Albumin, Bovine; D002701:Chloramphenicol
keywords: Immunoassays; Regeneration; SPR biosensor; Simultaneous detection