title: Morphological study of tooth development in podoplanin-deficient mice.
abstract: Podoplanin is a mucin-type highly O-glycosylated glycoprotein identified in several somatyic cells: podocytes, alveolar epithelial cells, lymphatic endothelial cells, lymph node stromal fibroblastic reticular cells, osteocytes, odontoblasts, mesothelial cells, glia cells, and others. It has been reported that podoplanin-RhoA interaction induces cytoskeleton relaxation and cell process stretching in fibroblastic cells and osteocytes, and that podoplanin plays a critical role in type I alveolar cell differentiation. It appears that podoplanin plays a number of different roles in contributing to cell functioning and growth by signaling. However, little is known about the functions of podoplanin in the somatic cells of the adult organism because an absence of podoplanin is lethal at birth by the respiratory failure. In this report, we investigated the tooth germ development in podoplanin-knockout mice, and the dentin formation in podoplanin-conditional knockout mice having neural crest-derived cells with deficiency in podoplanin by the Wnt1 promoter and enhancer-driven Cre recombinase: Wnt1-Cre;PdpnΔ/Δmice. In the Wnt1-Cre;PdpnΔ/Δmice, the tooth and alveolar bone showed no morphological abnormalities and grow normally, indicating that podoplanin is not critical in the development of the tooth and bone.
authors.affiliation: Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Kenyo
authors.initials: K
authors.lastname: Takara
authors.affiliation: Department of Odontology, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Maruo
authors.affiliation: Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Oka
authors.affiliation: Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kaji
authors.affiliation: Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hatakeyama
authors.affiliation: Department of Oral and Maxillofacial Surgery, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Naruhiko
authors.initials: N
authors.lastname: Sawa
authors.affiliation: Department of Regional Innovation, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Department of Oral and Maxillofacial Surgery, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Junro
authors.initials: J
authors.lastname: Yamashita
authors.affiliation: Department of Oral Growth & Development, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kojima
authors.affiliation: Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism; D002478:Cells, Cultured; D003804:Dentin / Q000196:embryology / Q000378:metabolism; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D007668:Kidney / Q000196:embryology / Q000473:pathology; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009432:Neural Crest / Q000378:metabolism; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology*; D009928:Organ Specificity; D050199:Podocytes / Q000378:metabolism / Q000648:ultrastructure; D012137:Respiratory System / Q000196:embryology / Q000378:metabolism; D013314:Stress, Mechanical; D014083:Tooth Germ / Q000196:embryology / Q000378:metabolism; D019076:Transgenes; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; C490441:Pdpn protein, mouse; D000098853:Podoplanin