title: Hepatoprotective activity of Butea monosperma bark against thioacetamide-induced liver injury in rats.
abstract: For thousands of years, the plant-based natural products have been a source of curative agents for various ailments. Butea monosperma (Fabaceae) has an important place in Indian traditional system of medicine for curing number of disorders. The present study deals with evaluation of hepatoprotective properties of ethyl acetate fraction (Beac) from B. monosperma bark in rat model. In preliminary antioxidant studies, Beac demonstrated pronounced superoxide scavenging (IC5088.85μg/ml) and anti-lipid peroxidation (IC50131.66μg/ml) potential. In animal studies, Beac showed protective effect against thioacetamide-induced pathophysiology in liver of male Wistar rats. The levels of different parameters related to hepatic functions were altered by thioacetamide treatment (300mg/g bw) in rats. The pre-treatment of rats with Beac (50, 100 and 200mg/kg bw) was able to normalize the biochemical markers viz. serum bilirubin, SGOT, SGPT, albumin and ALP along with liver antioxidative molecules viz. SOD, CAT, GSH and GR. Results of histopathological and colorimetric studies revealed that Beac treatment also restored the markers of fibrosis i.e. collagen and hydroxyproline towards normal level. Beac considerably inhibited thioacetamide-induced expression of p-PI3K, p-Akt and p-mTOR in hepatocytes as revealed from immunohistochemical studies. This finding is the first evidence of inhibitory action of B. monosperma bark on these pro-carcinogenic proteins. HRMS analysis revealed the presence of quercetin, buteaspermin B and ononin in Beac fraction of Butea monosperma. From the results, it can be concluded that B. monosperma bark is a rich source of phytochemicals with in vitro and in vivo protective activities which deserves further mechanistic studies for its use as a hepatoprotective agent in the prevention of hepatic inflammation and its related malignancies.
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India.
authors.forename: Varinder
authors.initials: V
authors.lastname: Kaur
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India; Akal College of Basic Sciences (Botany), Eternal University, Baru Sahib, Sirmour 173101, Himachal Pradesh, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India.
authors.forename: Paramjeet
authors.initials: P
authors.lastname: Kaur
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kaur
authors.affiliation: Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India.
authors.forename: Amrit Pal
authors.initials: AP
authors.lastname: Singh
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: sjkaur2011@gmail.com.
authors.forename: Satwinderjeet
authors.initials: S
authors.lastname: Kaur
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D031290:Butea / Q000737:chemistry*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013853:Thioacetamide / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D013853:Thioacetamide; D019869:Phosphatidylinositol 3-Kinases
keywords: Antioxidant; Butea monosperma; Hepatoprotection; PI3K/Akt/mTOR; Thioacetamide