title: Development of TaqMan RT-qPCR for the detection of type A human respiratory syncytial virus.
abstract: The human respiratory syncytial virus is a common respiratory pathogen in children. Improved diagnosis of the virus is dependent on the development of tools for the rapid detection and estimation of the viral loads. In the current study, RT-qPCR using TaqMan hydrolysis probe based on the F gene detection was developed to identify and quantify hRSV in clinical samples. The assay was validated by comparing the results with a commercially available RT-qPCR kit. The newly developed assay was sensitive in detecting hRSV positive samples (59/126) which were equivalent to those detected by the commercial kit (57/126) with a detection limit of 1 × 102copies/mL. A high correlation was found between the results of the newly developed assay and the commercial one. It was concluded that the newly developed RT-qPCR assay can be used as a sensitive detection tool for hRSV-A.
authors.affiliation: Microbiology Department, Virology Division, College of Medicine, Taif University, Al-Taif, 21944, Saudi Arabia; Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt. Electronic address: asa@tu.edu.sa.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: Abdel-Moneim
authors.affiliation: Biochemistry Department, College of Medicine, Taif University, Al-Taif, 21944, Saudi Arabia; Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
authors.forename: Gaber M
authors.initials: GM
authors.lastname: Shehab
authors.affiliation: Department of Paediatrics, College of Medicine, Taif University, Al-Taif, 21944, Saudi Arabia.
authors.forename: Adnan A
authors.initials: AA
authors.lastname: Alsulaimani
authors.affiliation: Hospital of Paediatrics, Al-Taif, 21944, Saudi Arabia.
authors.forename: Mater I R
authors.initials: MIR
authors.lastname: Al-Malky
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D012529:Saudi Arabia; D019562:Viral Load / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: Assay development; Human respiratory syncytial virus; RT-qPCR; Saudi Arabia; Virus detection; hRSV-A