title: MicroRNA-20b suppresses the expression of ZFP-148 in viral myocarditis.
abstract: Viral myocarditis is a common cardiovascular disease, which seriously endangers the health of people and even leads to sudden unexpected death. MicroRNAs play very important roles in various physical and pathological processes including cardiogenesis and heart diseases. In recent years, miR-20b has been implicated in various diseases such as breast cancer, gastric cancer, hepatocellular carcinoma, cardiovascular diseases. However, the function of miR-20b in the pathological progress of viral myocarditis has not been reported. In this study, we found that miR-20b was up-regulated in mouse heart tissues post Coxsackievirus B3 (CVB3) infection. Bioinformatics analysis identified ZFP-148, a transcription factor that plays essential roles in the regulation of virus replication, is one of the predicted targets of miR-20b. MiR-20b expression was found to be up-regulated and ZFP-148 protein level was markedly repressed during viral myocarditis. Further studies demonstrated that miR-20b directly binds to the 3'-UTR of ZFP-148 and suppresses its translation. Moreover, aberrant expression of miR-20b promoted the expression of anti-apoptosis proteins Bcl-2 and Bcl-xL, suggesting that altered gene expression might promote cardiomyocytes survival in viral myocarditis. Our findings indicated that miR-20b might be a potential therapeutic target for CVB3-induced viral myocarditis and a useful marker for the diagnosis of viral myocarditis.
authors.affiliation: Department of Forensic Medicine, Soochow University, Suzhou Dushuhu High Educational Town, Suzhou, Jiangsu, 215123, People's Republic of China. xuhongfei@suda.edu.cn.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xu
authors.affiliation: Department of Forensic Medicine, Soochow University, Suzhou Dushuhu High Educational Town, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Xiang-Ting
authors.initials: XT
authors.lastname: Gao
authors.affiliation: Department of Forensic Medicine, Shanghai Medical College, Fudan University, Xuhui, Shanghai, 200032, People's Republic of China.
authors.forename: Jun-Yi
authors.initials: JY
authors.lastname: Lin
authors.affiliation: Department of Forensic Medicine, Soochow University, Suzhou Dushuhu High Educational Town, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Xue-Hua
authors.initials: XH
authors.lastname: Xu
authors.affiliation: Department of Forensic Medicine, Soochow University, Suzhou Dushuhu High Educational Town, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of dermatological, The second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China. 45757669@qq.com.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Ding
authors.affiliation: Department of Forensic Medicine, Soochow University, Suzhou Dushuhu High Educational Town, Suzhou, Jiangsu, 215123, People's Republic of China. whhongfei@sina.com.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Zhu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D017209:Apoptosis; D003384:Coxsackievirus Infections / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009205:Myocarditis / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; D035683:MicroRNAs; C532375:Mirn20 microRNA, mouse; D014157:Transcription Factors; C491741:Zfp148 protein, mouse
keywords: Viral myocarditis; ZFP-148; miR-20b