title: [Effect of Thrombocytopenia on Migration and Homing Pattern of Dendritic Cells].
abstract: OBJECTIVE
  To investigate the effect of thrombocytopenia on the migration patterns of adoptive dendritic cell(DC) in vivo.
  
  
  METHODS
  The mouse model of thrombocytopenia was established by intraperitoneal administration of anti-CD41 mAb MWReg30. Mouse bone marrow(BM)-derived DC were injected into thrombocytopenia mouse by footpad infusion and intravenous infusion. The DC migration and distribution pattern were detected by bioluminescence imaging.
  
  
  RESULTS
  More than 80% platelets were cleared in the experimental group which was infused with anti-CD41 antibody. At 72 h after injection, the percentage of injected DC that migrated from footpad to popliteal lymph nodes(PLNs) and inguinal lymph nodes(ILNs) were (0.32±0.02)% and (0.02±0.01)% in experimental group, and (0.27±0.15)% and (0.02±0.02)% in control group, respectively. Statistic data showed that there was no statistical difference between these 2 groups (P>0.05). The issue distribution pattern of intravenously injected DC between experimental group and control group were not distinctly different, and large amounts of injected DC accumulated in the spleen, liver draining lymph-nodes lungs and liver.
  
  
  CONCLUSIONS
  Thrombocytopenia has not a distinct effect on the migratory capacity and tissue distribution of DC by either footpad or intravenous injection.
authors.affiliation: Guangxi Medical University, Nanning 530021, Guangxi Province,China; Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Zhou
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Chu-Lin
authors.initials: CL
authors.lastname: He
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail:lovechina1980@163.com.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Guangxi Medical University, Nanning 530021, Guangxi Province,China; Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: lszhan91@yahoo.com.
authors.forename: Lin-Sheng
authors.initials: LS
authors.lastname: Zhan
mesh_terms: D000818:Animals; D002465:Cell Movement; D003713:Dendritic Cells / Q000502:physiology*; D008198:Lymph Nodes; D051379:Mice; D008810:Mice, Inbred C57BL; D013921:Thrombocytopenia