title: Antioxidant treatment ameliorates diabetes-induced dysfunction of the vas deferens in a rat model.
abstract: Diabetes mellitus (DM) affects the male ejaculatory function. This study was designed to evaluate the role of oxidative stress in the development of diabetes-induced dysfunction of vas deferens (VD) in the rat. DM was induced by streptozotocin in 40 male Wistar rats. Subsequently, the diabetic animals were divided into three groups: DM group, DM + Eda group and DM + Tau group. These groups were administered saline, edaravone and taurine, respectively, daily for 4 weeks. Another group of ten rats served as a control group. DM was diagnosed in the 40 streptozotocin-injected rats. DM significantly reduced the VD weight. Additionally, DM induced in vitro VD hypercontractility, VD histological abnormalities and increased the serum and VD tissue concentration of malondialdehyde. VD immunohistochemistry revealed overexpression of three markers of oxidative stress. DM significantly reduced serum testosterone levels. No live birth was documented in all DM rats in mating experiments. Antioxidants significantly improved all the aforementioned parameters, except the testosterone levels. This study indicates a deleterious impact of DM-induced oxidative stress on VD histological and functional features. Antioxidant treatment may provide an adjunct tool to alleviate ejaculatory disorders for male patients with type 1 diabetes.
authors.affiliation: Division of Urology, Department of Surgery, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: P
authors.identifier: http://orcid.org/0000-0002-6202-8653
authors.initials: P
authors.lastname: Tsounapi
authors.affiliation: Division of Urology, Department of Surgery, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Honda
authors.affiliation: Department of Urology, School of Medicine, University of Ioannina, Ioannina, Greece.
authors.forename: F
authors.initials: F
authors.lastname: Dimitriadis
authors.affiliation: Department of Pharmacology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.affiliation: Division of Organ Pathology, Department of Pathology, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shiomi
authors.affiliation: Division of Urology, Department of Surgery, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hikita
authors.affiliation: Department of Pharmacology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.affiliation: Department of Pharmacology, Graduate School of Medicine, Osaka City University, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tomita
authors.affiliation: Department of Urology, School of Medicine, University of Ioannina, Ioannina, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Sofikitis
authors.affiliation: Division of Urology, Department of Surgery, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Takenaka
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D000983:Antipyrine / Q000031:analogs & derivatives / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology*; D000077553:Edaravone; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology; D014649:Vas Deferens / Q000187:drug effects* / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D013654:Taurine; D008315:Malondialdehyde; D000077553:Edaravone; D000983:Antipyrine
keywords: contractions; diabetes mellitus; oxidative stress; testosterone; vas deferens