title: Progranulin Protects Against Osteonecrosis of the Femoral Head by Activating ERK1/2 Pathway.
abstract: The aim of this study was to investigate progranulin (PGRN) expression and its effect in cartilage degradation and in the pathogenesis of osteonecrosis of the femoral head (ONFH). Cartilage specimens were obtained from ONFH and FNF patients and PGRN expression was analyzed by immunohistochemistry, western blot analysis, and RT-PCR. Peripheral blood PGRN level was detected by ELISA. Additionally, primary chondrocytes were cultured and treated with PGRN. Next, the expression of aggrecan and collagen II and the activation of ERK1/2 were detected. We observed that the expression of PGRN was significantly upregulated in ONFH patients' articular cartilage, and recombinant PGRN could promote expression of aggrecan and collagen II and the activation of ERK1/2. Collectively, PGRN can improve chondrocyte anabolism and perform a therapeutic role in the pathogenesis of ONFH. This study helps to elucidate the pathogenesis of ONFH and presents PGRN as a potential target for the treatment of ONFH.
authors.affiliation: Department of Orthopaedic Surgery, Shandong University Qilu Hospital, No. 107, Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Yingguang
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Orthopaedic Surgery, Shandong University Qilu Hospital, No. 107, Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Si
authors.affiliation: Department of Orthopaedic Surgery, Shandong University Qilu Hospital, No. 107, Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedic Surgery, Shandong University Qilu Hospital, No. 107, Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, Shandong University Qilu Hospital, No. 107, Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Kaiyuan
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Orthopaedic Surgery, Taian Central Hospital, Taian, Shandong, 271000, China.
authors.forename: Yuedong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic Surgery, Shandong University Qilu Hospital, No. 107, Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Orthopaedic Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, No. 16766, Jingshi Road, Jinan, Shandong, 250014, People's Republic of China. 44751061@qq.com.
authors.forename: Jingkun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, Shandong University Qilu Hospital, No. 107, Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China. 2640884218@qq.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Nie
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D002358:Cartilage, Articular; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005270:Femur Head / Q000473:pathology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D010020:Osteonecrosis / Q000517:prevention & control*; D000077153:Progranulins; D020011:Protective Agents / Q000494:pharmacology
chemical_list: D053674:Aggrecans; C492314:GRN protein, human; D036341:Intercellular Signaling Peptides and Proteins; D000077153:Progranulins; D020011:Protective Agents; D003094:Collagen
keywords: ERK; TNF-α; osteonecrosis; progranulin