title: A Gradient of Glycolytic Activity Coordinates FGF and Wnt Signaling during Elongation of the Body Axis in Amniote Embryos.
abstract: Mammalian embryos transiently exhibit aerobic glycolysis (Warburg effect), a metabolic adaptation also observed in cancer cells. The role of this particular type of metabolism during vertebrate organogenesis is currently unknown. Here, we provide evidence for spatiotemporal regulation of glycolysis in the posterior region of mouse and chicken embryos. We show that a posterior glycolytic gradient is established in response to graded transcription of glycolytic enzymes downstream of fibroblast growth factor (FGF) signaling. We demonstrate that glycolysis controls posterior elongation of the embryonic axis by regulating cell motility in the presomitic mesoderm and by controlling specification of the paraxial mesoderm fate in the tail bud. Our results suggest that glycolysis in the tail bud coordinates Wnt and FGF signaling to promote elongation of the embryonic axis.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Université de Strasbourg, Illkirch 67400, France; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Department of Pathology, Brigham and Women's Hospital, 60 Fenwood Road, Boston, MA 02115, USA.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Oginuma
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Université de Strasbourg, Illkirch 67400, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Moncuquet
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Department of Pathology, Brigham and Women's Hospital, 60 Fenwood Road, Boston, MA 02115, USA.
authors.forename: Fengzhu
authors.initials: F
authors.lastname: Xiong
authors.affiliation: Metabolon, 617 Davis Drive, Suite 400, Morrisville, NC 27560, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Karoly
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Université de Strasbourg, Illkirch 67400, France; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Department of Pathology, Brigham and Women's Hospital, 60 Fenwood Road, Boston, MA 02115, USA.
authors.forename: Jérome
authors.initials: J
authors.lastname: Chal
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Université de Strasbourg, Illkirch 67400, France; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Department of Pathology, Brigham and Women's Hospital, 60 Fenwood Road, Boston, MA 02115, USA.
authors.forename: Karine
authors.initials: K
authors.lastname: Guevorkian
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Université de Strasbourg, Illkirch 67400, France; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Department of Pathology, Brigham and Women's Hospital, 60 Fenwood Road, Boston, MA 02115, USA. Electronic address: pourquie@genetics.med.harvard.edu.
authors.forename: Olivier
authors.initials: O
authors.lastname: Pourquié
mesh_terms: D000650:Amnion / Q000196:embryology* / Q000378:metabolism*; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D002465:Cell Movement; D002642:Chick Embryo; D005110:Extracellular Space / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D006019:Glycolysis / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism; D051379:Mice; D010641:Phenotype; D013623:Tail / Q000196:embryology; D014158:Transcription, Genetic; D014714:Vertebrates / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000235:genetics
chemical_list: D005346:Fibroblast Growth Factors
keywords: FGF; axial elongation; embryo; glycolysis; lactate; metabolism; paraxial mesoderm; somitogenesis