title: Metagenomic analysis of the microbiome in three different bioreactor configurations applied to commercial laundry wastewater treatment.
abstract: The taxonomic and functional diversity of three different biological reactors (fluidized bed reactor, FBR; up-flow anaerobic sludge blanket reactor, UASB; and expanded granular sludge bed reactor, EGSB) used for commercial laundry wastewater treatment was investigated using metagenome shotgun sequencing. Metagenomes were sequenced on the Illumina Hiseq platform and were analyzed using MG-RAST, STAMP and PAST software. The EGSB and UASB reactors were more closely related based on taxonomic and functional profiles, likely due to similar granular sludge and procedures adopted to ensure anaerobic conditions. The EGSB and UASB reactors showed a predominance of methanogens and genes related to methanogenesis, with a prevalence of the acetoclastic pathway, in addition to the peripheral and central O2-independent pathways for aromatic compound degradation. By contrast, FBR showed a dominance of aerobic microbiota and pathways for O2-dependent aromatic compound degradation. Therefore, although the reactors showed similar surfactant removal levels, the microbial composition, functional diversity and aromatic compound degradation pathways were significantly distinct.
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, SP CEP 13081-970, Brazil. Electronic address: palladino@sc.usp.br.
authors.forename: T P
authors.initials: TP
authors.lastname: Delforno
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, SP CEP 13081-970, Brazil. Electronic address: gilenolacerdajr@gmail.com.
authors.forename: G V
authors.initials: GV
authors.lastname: Lacerda
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, SP CEP 13081-970, Brazil. Electronic address: inatalia.sierra@gmail.com.
authors.forename: I N
authors.initials: IN
authors.lastname: Sierra-Garcia
authors.affiliation: School of Technology, Division of Technology in Environment Sanitation, Campinas University - UNICAMP, Limeira, SP CEP 13484-332, Brazil. Electronic address: dagokada@gmail.com.
authors.forename: D Y
authors.initials: DY
authors.lastname: Okada
authors.affiliation: Laboratory of Biological Processes, Department of Hydraulics and Sanitation, Engineering School of São Carlos - University of São Paulo (EESC - USP), Campus II, São Carlos, SP CEP 13563-120, Brazil. Electronic address: thazmacedo@gmail.com.
authors.forename: T Z
authors.initials: TZ
authors.lastname: Macedo
authors.affiliation: Laboratory of Biological Processes, Department of Hydraulics and Sanitation, Engineering School of São Carlos - University of São Paulo (EESC - USP), Campus II, São Carlos, SP CEP 13563-120, Brazil. Electronic address: varesche@sc.usp.br.
authors.forename: M B A
authors.initials: MB
authors.lastname: Varesche
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, SP CEP 13081-970, Brazil. Electronic address: vmaia@cpqba.unicamp.br.
authors.forename: V M
authors.initials: VM
authors.lastname: Oliveira
keywords: Aromatic compound degradation pathways; Illumina sequencing, genetic potential; Linear alkylbenzene sulfonate (LAS)