title: Screening of Six Medicinal Plant Extracts Obtained by Two Conventional Methods and Supercritical CO₂ Extraction Targeted on Coumarin Content, 2,2-Diphenyl-1-picrylhydrazyl Radical Scavenging Capacity and Total Phenols Content.
abstract: Six medicinal plantsHelichrysum italicum(Roth) G. Don,Angelica archangelicaL.,Lavandula officinalisL.,Salvia officinalisL.,Melilotus officinalisL., andRuta graveolensL. were used. The aim of the study was to compare their extracts obtained by Soxhlet (hexane) extraction, maceration with ethanol (EtOH), and supercritical CO₂ extraction (SC-CO₂) targeted on coumarin content (by high performance liquid chromatography with ultraviolet detection, HPLC-UV), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging capacity, and total phenols (TPs) content (by Folin-Ciocalteu assay). The highest extraction yields were obtained by EtOH, followed by hexane and SC-CO₂. The highest coumarin content (316.37 mg/100 g) was found inM. officinalisEtOH extracts, but its SC-CO₂ extraction yield was very low for further investigation. Coumarin was also found in SC-CO₂ extracts ofS. officinalis,R. graveolens,A. archangelica, andL. officinalis. EtOH extracts of all plants exhibited the highest DPPH scavenging capacity. SC-CO₂ extracts exhibited antiradical capacity similar to hexane extracts, whileS. officinalisSC-CO₂ extracts were the most potent (95.7%). EtOH extracts contained the most TPs (up to 132.1 mg gallic acid equivalents (GAE)/g fromH. italicum) in comparison to hexane or SC-CO₂ extracts. TPs content was highly correlated to the DPPH scavenging capacity of the extracts. The results indicate that for comprehensive screening of different medicinal plants, various extraction techniques should be used in order to get a better insight into their components content or antiradical capacity.
authors.affiliation: Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, 31000 Osijek, Croatia. mmolnar@ptfos.hr.
authors.forename: Maja
authors.initials: M
authors.lastname: Molnar
authors.affiliation: Faculty of Chemistry and Technology, University of Split, R. Boškovića 35, 21000 Split, Croatia. igor@ktf-split.hr.
authors.forename: Igor
authors.initials: I
authors.lastname: Jerković
authors.affiliation: Department of Clinical Laboratory Diagnostics, University Hospital Centre Osijek, Huttlerova 4, 31000 Osijek, Croatia. dragica.suknovic@gmail.com.
authors.forename: Dragica
authors.initials: D
authors.lastname: Suknović
authors.affiliation: Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, 31000 Osijek, Croatia. bbilic@ptfos.hr.
authors.forename: Blanka
authors.initials: B
authors.lastname: Bilić Rajs
authors.affiliation: Croatian Veterinary Institute, Branch, Veterinary Institute Vinkovci, Josipa Kozarca 24, 32100 Vinkovci, Croatia. k2aladic@gmail.com.
authors.forename: Krunoslav
authors.initials: K
authors.lastname: Aladić
authors.affiliation: Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, 31000 Osijek, Croatia. drago.subaric@ptfos.hr.
authors.forename: Drago
authors.initials: D
authors.lastname: Šubarić
authors.affiliation: Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, 31000 Osijek, Croatia. stela.jokic@ptfos.hr.
authors.forename: Stela
authors.initials: S
authors.lastname: Jokić
mesh_terms: D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D003374:Coumarins / Q000737:chemistry* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D003374:Coumarins; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts
keywords: antiradical capacity; coumarin; extraction; medicinal plants; supercritical CO2 extraction