title: Essential Oil of Aristolochia trilobata: Synthesis, Routes of Exposure, Acute Toxicity, Binary Mixtures and Behavioral Effects on Leaf-Cutting Ants.
abstract: Plants of the genusAristolochiahave been frequently reported as important medicinal plants. Despite their high bioactive potential, to date, there are no reports of their effects on leaf-cutting ants. Therefore, the present study aimed to evaluate the insecticidal activity of the essential oil ofAristolochia trilobataand its major components onAtta sexdensandAcromyrmex balzani, two species of leaf-cutting ants. The bioassays were performed regarding routes of exposure, acute toxicity, binary mixtures of the major components and behavioral effects. Twenty-five components were identified in the essential oil ofA. trilobatausing a gas chromatographic system equipped with a mass spectrometer and a flame ionization detector. The components found in higher proportions were sulcatyl acetate, limonene,p-cymene and linalool. The essential oil ofA. trilobataand its individual major components were efficient againstA. balzaniandA. sexdensworkers when applied by fumigation. These components showed fast and efficient insecticidal activity on ants. The components acted synergistically and additively onA. balzaniandA. sexdens, respectively, and caused a strong repellency/irritability in the ants. Thus, our results demonstrate the great potential of the essential oil ofA. trilobataand its major components for the development of new insecticides.
authors.affiliation: Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. bruna_barreiros02@hotmail.com.
authors.forename: Bruna Maria S
authors.initials: BM
authors.lastname: de Oliveira
authors.affiliation: Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. carlisson_melo@hotmail.com.
authors.forename: Carlisson R
authors.initials: CR
authors.lastname: Melo
authors.affiliation: Departamento de Química, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. periclesbalves@gmail.com.
authors.forename: Péricles B
authors.initials: PB
authors.lastname: Alves
authors.affiliation: Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. abraaoufs@gmail.com.
authors.forename: Abraão A
authors.initials: AA
authors.lastname: Santos
authors.affiliation: Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. aneccelestinos.18@gmail.com.
authors.forename: Ane Caroline C
authors.initials: AC
authors.lastname: Santos
authors.affiliation: Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. alisson.0910.silva@gmail.com.
authors.forename: Alisson da S
authors.initials: AD
authors.lastname: Santana
authors.affiliation: Departamento de Ecologia, Universidade Federal de Sergipe, São Cristóvão 491000-000, Brazil. anatermes@gmail.com.
authors.forename: Ana Paula A
authors.initials: AP
authors.lastname: Araújo
authors.affiliation: Departamento de Química, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. pedro_ellison@hotmail.com.
authors.forename: Pedro E S
authors.initials: PE
authors.lastname: Nascimento
authors.affiliation: Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. arie.blank@gmail.com.
authors.forename: Arie F
authors.initials: AF
authors.lastname: Blank
authors.affiliation: Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão 49100-000, Brazil. bacci.ufs@gmail.com.
authors.forename: Leandro
authors.initials: L
authors.lastname: Bacci
mesh_terms: D000818:Animals; D001000:Ants / Q000187:drug effects* / Q000502:physiology; D030022:Aristolochia / Q000737:chemistry*; D001522:Behavior, Animal / Q000187:drug effects; D002849:Chromatography, Gas; D005651:Fumigation; D007306:Insecticides / Q000737:chemistry* / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology
chemical_list: D007306:Insecticides; D009822:Oils, Volatile; D010938:Plant Oils
keywords: Aristolochiaceae; Formicidae; additive effect; fumigation; insecticidal plant; monoterpenes; repellency; synergism