title: Pseudomonas aeruginosa produces aspirin insensitive eicosanoids and contributes to the eicosanoid profile of polymicrobial biofilms with Candida albicans.
abstract: The interaction of clinically relevant microorganisms is the focus of various studies, e.g. the interaction between the pathogenic yeast, Candida albicans, and the bacterium, Pseudomonas aeruginosa. During infection both release arachidonic acid, which they can transform into eicosanoids. This study evaluated the production of prostaglandin E2, prostaglandin F2αand 15-hydroxyeicosatetraenoic acid by biofilms of P. aeruginosa and C. albicans. The influence of co-incubation, acetylsalicylic acid and nordihydroguaiaretic acid on biofilm formation and eicosanoid production was evaluated. Acetylsalicylic acid decreased colony forming units of P. aeruginosa, but increased metabolic activity and eicosanoid production of the cells. In contrast to prostaglandin E2, prostaglandin F2aproduction by C. albicans was insensitive to acetylsalicylic acid, indicating that different enzymes are responsible for their production in this yeast. Nordihydroguaiaretic acid inhibited biofilm formation by P. aeruginosa, however co-incubation provided protection against this inhibitor. Production of these eicosanoids could affect pathogen-clearance and infection dynamics and this previously uncharacterized facet of interaction could facilitate novel therapeutic intervention against polymicrobial infection.
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
authors.forename: Ruan
authors.initials: R
authors.lastname: Fourie
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa; National Control Laboratory for Biological Products, University of the Free State, Bloemfontein, South Africa.
authors.forename: Ruan
authors.initials: R
authors.lastname: Ells
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
authors.forename: Gabré
authors.initials: G
authors.lastname: Kemp
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
authors.forename: Olihile M
authors.initials: OM
authors.lastname: Sebolai
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
authors.forename: Jacobus
authors.initials: J
authors.lastname: Albertyn
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa. Electronic address: PohlCH@ufs.ac.za.
authors.forename: Carolina H
authors.initials: CH
authors.lastname: Pohl
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism*; D001241:Aspirin / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D002176:Candida albicans / Q000502:physiology*; D002853:Chromatography, Liquid; D015232:Dinoprostone / Q000378:metabolism; D015777:Eicosanoids / Q000032:analysis* / Q000378:metabolism; D009637:Masoprocol / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology*; D053719:Tandem Mass Spectrometry
chemical_list: D015777:Eicosanoids; D016718:Arachidonic Acid; D009637:Masoprocol; D015232:Dinoprostone; D001241:Aspirin
keywords: Acetylsalicylic acid; Biofilm; Candida albicans; Eicosanoid; Nordihydroguaiaretic acid; Pseudomonas aeruginosa