title: Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in China.
abstract: In recent years, novel hepadnaviruses, hepeviruses, hepatoviruses, and hepaciviruses have been discovered in various species of bat around the world, indicating that bats may act as natural reservoirs for these hepatitis viruses. In order to further assess the distribution of hepatitis viruses in bat populations in China, we tested the presence of these hepatitis viruses in our archived bat liver samples that originated from several bat species and various geographical regions in China.
  
  
  
  A total of 78 bat liver samples (involving two families, five genera, and 17 species of bat) were examined using nested or heminested reverse transcription PCR (RT-PCR) with degenerate primers. Full-length genomic sequences of two virus strains were sequenced followed by phylogenetic analyses.
  
  
  
  Four samples were positive for hepadnavirus, only one was positive for hepevirus, and none of the samples were positive for hepatovirus or hepacivirus. The hepadnaviruses were discovered in the horseshoe bats, Rhinolophus sinicus and Rhinolophus affinis, and the hepevirus was found in the whiskered bat Myotis davidii. The full-length genomic sequences were determined for one of the two hepadnaviruses identified in R. sinicus (designated BtHBVRs3364) and the hepevirus (designated BtHEVMd2350). A sequence identity analysis indicated that BtHBVRs3364 had the highest degree of identity with a previously reported hepadnavirus from the roundleaf bat, Hipposideros pomona, from China, and BtHEVMd2350 had the highest degree of identity with a hepevirus found in the serotine bat, Eptesicus serotinus, from Germany, but it exhibited high levels of divergence at both the nucleotide and the amino acid levels.
  
  
  
  This is the first study to report that the Chinese horseshoe bat and the Chinese whiskered bat have been found to carry novel hepadnaviruses and a novel hepevirus, respectively. The discovery of BtHBVRs3364 further supports the significance of host switches evolution while opposing the co-evolutionary theory associated with hepadnaviruses. According to the latest criterion of the International Committee on Taxonomy of Viruses (ICTV), we hypothesize that BtHEVMd2350 represents an independent genotype within the species Orthohepevirus D of the family Hepeviridae.
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.|Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xing-Lou
authors.initials: XL
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Ge
authors.affiliation: Guangdong Institute of Applied Biological Resource, Guangzhou, 510260, China.
authors.forename: Li-Biao
authors.initials: LB
authors.lastname: Zhang
authors.affiliation: Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
authors.forename: Yun-Zhi
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany.
authors.forename: Claus-Thomas
authors.initials: CT
authors.lastname: Bock
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. zlshi@wh.iov.cn.
authors.forename: Zheng-Li
authors.initials: ZL
authors.lastname: Shi
mesh_terms: D000818:Animals; D002681:China; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D016679:Genome, Viral; D015613:Hepadnaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D052678:Hepevirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008099:Liver / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
keywords: Bat; Genome characterization; Hepadnavirus; Hepevirus; Natural reservoir