title: Reconstruction of Ligament and Tendon Defects Using Cell Technologies.
abstract: We studied the possibility of restoring the integrity of the Achilles tendon in rabbits using autologous multipotent stromal cells. Collagen or gelatin sponges populated with cells were placed in a resorbable Vicryl mesh tube and this tissue-engineered construct was introduced into a defect of the middle part of the Achilles tendon. In 4 months, histological analysis showed complete regeneration of the tendon with the formation of parallel collagen fibers, spindle-shaped tenocytes, and newly formed vessels.
authors.affiliation: N. F. Gamaleya Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. rubenchail@yandex.ru.
authors.forename: R K
authors.initials: RK
authors.lastname: Chailakhyan
authors.affiliation: I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Shekhter
authors.affiliation: I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Ivannikov
authors.affiliation: I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Tel'pukhov
authors.affiliation: I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Suslin
authors.affiliation: N. F. Gamaleya Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Gerasimov
authors.affiliation: I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Tonenkov
authors.affiliation: N. F. Gamaleya Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Grosheva
authors.affiliation: I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: P V
authors.initials: PV
authors.lastname: Panyushkin
authors.affiliation: N. F. Gamaleya Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: I L
authors.initials: IL
authors.lastname: Moskvina
authors.affiliation: Institute of Photonics Technologies, Russian Academy of Sciences, Federal Research Center "Crystallography and Photonics", Russian Academy of Sciences, Moscow, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Vorob'eva
authors.affiliation: Institute of Photonics Technologies, Russian Academy of Sciences, Federal Research Center "Crystallography and Photonics", Russian Academy of Sciences, Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Bagratashvili
mesh_terms: D000125:Achilles Tendon / Q000293:injuries / Q000601:surgery* / Q000648:ultrastructure; D000818:Animals; D001696:Biomechanical Phenomena; D003094:Collagen / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D008022:Ligaments / Q000293:injuries / Q000601:surgery* / Q000648:ultrastructure; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D011098:Polyglactin 910 / Q000737:chemistry; D011817:Rabbits; D019651:Plastic Surgery Procedures / Q000295:instrumentation / Q000379:methods*; D000070916:Tenocytes / Q000166:cytology / Q000502:physiology; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000502:physiology*
chemical_list: D011098:Polyglactin 910; D005780:Gelatin; D003094:Collagen
keywords: ligaments and tendons; multipotent stromal cells; regeneration; tissue-engineered construct