title: Mineral status in canine medial coronoid process disease: a cohort study using analysis of hair by mass spectrometry.
abstract: In several species, developmental skeletal diseases involving abnormal endochondral ossification have been associated with imbalanced mineral intake. Hair analysis reflects long-term mineral status. To determine the mineral content of hair from dogs with or without medial coronoid process disease (MCPD). Dogs with MCPD have a different profile of minerals known to influence metalloenzymes involved in endochondral ossification. After cleansing, chelation and acid digestion of hair samples (n=79 in total: control dogs, n=70 v MCPD, n=9), mineral profile (7 major and 25 trace elements) was determined by inductively coupled plasma-mass spectrometry. Dogs were of similar age (control, 4.05 [1.85-7.70] v MCPD, 4.30 [3.25-6.53] median (IQR) years; P=0.78) and gender (control, n=43/27 v MCPD, n=4/5 males/females). 28/70 (40 per cent) of control and 8/9 (88 per cent) of MCPD dogs were neutered, respectively. Hair from dogs with MCPD contained significantly lower amounts (µg/g/DM) of copper, sulphur and zinc (all at P<0.001). Age, sex and neutered status had no effect on hair mineral status. Based on hair analysis, a role for mineral imbalance including copper, sulphur and zinc in the aetiopathogenesis of canine MCPD is suggested. Hair mineral analysis may prove useful as a biomarker for susceptible puppies.
authors.affiliation: Provet Limited, The Old School House, 7 Church lane, Ravenfield, South Yorkshire S65 4NG, UK.
authors.forename: M
authors.initials: M
authors.lastname: Davies
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12, UK.
authors.forename: J
authors.initials: J
authors.lastname: West
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12, UK.
authors.forename: C
authors.initials: C
authors.lastname: Williams
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12, UK.
authors.forename: D S
authors.initials: DS
authors.lastname: Gardner
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D015331:Cohort Studies; D003300:Copper / Q000032:analysis; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006197:Hair / Q000737:chemistry*; D007592:Joint Diseases / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D013058:Mass Spectrometry / Q000662:veterinary; D008903:Minerals / Q000032:analysis*; D013455:Sulfur / Q000032:analysis; D015032:Zinc / Q000032:analysis
chemical_list: D008903:Minerals; D013455:Sulfur; D003300:Copper; D015032:Zinc
keywords: copper; medial coronoid process disease; minerals; zinc