title: Synthesis of Reusable Silica Nanosphere-Supported Pt(IV) Complex for Formation of Disulfide Bonds in Peptides.
abstract: Some peptide-based drugs, including oxytocin, vasopressin, ziconotide, pramlintide, nesiritide, and octreotide, contain one intramolecular disulfide bond. A novel and reusable monodispersed silica nanosphere-supported Pt(IV) complex (SiO₂@TPEA@Pt(IV)); TPEA:N-[3-(trimethoxysilyl)propyl]ethylenediamine) was synthesized via a four-step procedure and was used for the formation of intramolecular disulfide bonds in peptides. Transmission electron microscopy (TEM) and chemical mapping results for the Pt(II) intermediates and for SiO₂@TPEA@Pt(IV) show that the silica nanospheres possess a monodisperse spherical structure and contain uniformly-distributed Si, O, C, N, Cl, and Pt. The valence state of Pt on the silica nanospheres was characterized by X-ray photoelectron spectroscopy (XPS). The Pt(IV) loaded on SiO₂@TPEA@Pt(IV) was 0.15 mmol/g, as determined by UV-VIS spectrometry. The formation of intramolecular disulfides in six dithiol-containing peptides of variable lengths by the use of SiO₂@TPEA@Pt(IV) was investigated, and the relative oxidation yields were determined by high-performance liquid chromatography (HPLC). In addition, peptide1(Ac-CPFC-NH₂) was utilized to study the reusability of SiO₂@TPEA@Pt(IV). No significant decrease in the relative oxidation yield was observed after ten reaction cycles. Moreover, the structure of SiO₂@TPEA@Pt(IV) after being used for ten cycles was determined to be similar to its initial one, demonstrating the cycling stability of the complex.
authors.affiliation: College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, and the MOE Key Laboratory of Medicinal Chemistry and Molecular Diagnostics, Hebei University, No. 180, Wusi Dong Road, Baoding 071002, China. 18233272151@163.com.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Hou
authors.affiliation: College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, and the MOE Key Laboratory of Medicinal Chemistry and Molecular Diagnostics, Hebei University, No. 180, Wusi Dong Road, Baoding 071002, China. wjswbys@126.com.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, and the MOE Key Laboratory of Medicinal Chemistry and Molecular Diagnostics, Hebei University, No. 180, Wusi Dong Road, Baoding 071002, China. 15231212708@163.com.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, and the MOE Key Laboratory of Medicinal Chemistry and Molecular Diagnostics, Hebei University, No. 180, Wusi Dong Road, Baoding 071002, China. scyhbu@126.com.
authors.forename: Aiying
authors.initials: A
authors.lastname: Han
authors.affiliation: College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, and the MOE Key Laboratory of Medicinal Chemistry and Molecular Diagnostics, Hebei University, No. 180, Wusi Dong Road, Baoding 071002, China. shuyinghuo@126.com.
authors.forename: Shuying
authors.initials: S
authors.lastname: Huo
authors.affiliation: College of Chemistry and Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, and the MOE Key Laboratory of Medicinal Chemistry and Molecular Diagnostics, Hebei University, No. 180, Wusi Dong Road, Baoding 071002, China. shensg@hbu.edu.cn.
authors.forename: Shigang
authors.initials: S
authors.lastname: Shen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056831:Coordination Complexes / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D010984:Platinum / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D004220:Disulfides; D016877:Oxidants; D010455:Peptides; D013438:Sulfhydryl Compounds; D010984:Platinum; D012822:Silicon Dioxide
keywords: intramolecular disulfide; monodisperse silica nanospheres; peptide; reusability; supported platinum(IV) complex