title: New Mycobacterium tuberculosis Complex Sublineage, Brazzaville, Congo.
abstract: Tuberculosis is a leading cause of illness and death in Congo. No data are available about the population structure and transmission dynamics of the Mycobacterium tuberculosis complex strains prevalent in this central Africa country. On the basis of single-nucleotide polymorphisms detected by whole-genome sequencing, we phylogenetically characterized 74 MTBC isolates from Brazzaville, the capital of Congo. The diversity of the study population was high; most strains belonged to the Euro-American lineage, which split into Latin American Mediterranean, Uganda I, Uganda II, Haarlem, X type, and a new dominant sublineage named Congo type (n = 26). Thirty strains were grouped in 5 clusters (each within 12 single-nucleotide polymorphisms), from which 23 belonged to the Congo type. High cluster rates and low genomic diversity indicate recent emergence and transmission of the Congo type, a new Euro-American sublineage of MTBC.
authors.forename: Sven
authors.initials: S
authors.lastname: Malm
authors.forename: Laure S Ghoma
authors.initials: LS
authors.lastname: Linguissi
authors.forename: Emmanuel M
authors.initials: EM
authors.lastname: Tekwu
authors.forename: Jeannhey C
authors.initials: JC
authors.lastname: Vouvoungui
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Kohl
authors.forename: Patrick
authors.initials: P
authors.lastname: Beckert
authors.forename: Anissa
authors.initials: A
authors.lastname: Sidibe
authors.forename: Sabine
authors.initials: S
authors.lastname: Rüsch-Gerdes
authors.forename: Igor K
authors.initials: IK
authors.lastname: Madzou-Laboum
authors.forename: Sylvie
authors.initials: S
authors.lastname: Kwedi
authors.forename: Véronique
authors.initials: V
authors.lastname: Penlap Beng
authors.forename: Matthias
authors.initials: M
authors.lastname: Frank
authors.forename: Francine
authors.initials: F
authors.lastname: Ntoumi
authors.forename: Stefan
authors.initials: S
authors.lastname: Niemann
mesh_terms: D000328:Adult; D016000:Cluster Analysis; D003223:Congo / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*; D055815:Young Adult
keywords: Brazzaville; Congo; Genetic diversity; Mycobacterium tuberculosis; bacteria; epidemiology; phylogeny; transmission; tuberculosis and other mycobacteria