title: Molecular Subtyping and Source Attribution of Campylobacter Isolated from Food Animals.
abstract: Campylobacter spp. commonly cause gastrointestinal illness in humans. Poultry meats have long been considered the predominant source of these infections, but few in-depth Campylobacter source attribution studies have been completed. We analyzed more than 1,300 Campylobacter isolates recovered from a number of animal and food sources, including dairy and beef cattle, pigs, poultry, and retail poultry meat, and compared them with Campylobacter isolates recovered from human clinical samples. Each isolate was subtyped using pulsed-field gel electrophoresis (PFGE) with SmaI and queried against the Centers for Disease Control and Prevention PulseNet database to identify human isolates with indistinguishable patterns. Half (49.5%) of the PFGE patterns from poultry animal and retail meat isolates were indistinguishable from patterns of at least one human isolate. Among the isolates from beef and dairy cows, 56.6 and 65.0%, respectively, of their PFGE patterns were indistinguishable from those of human isolates. Only a small portion of the PFGE patterns of Campylobacter isolated from pigs (9.5%) were found to have PFGE patterns in common with human isolates. These data imply that cattle may be larger contributors to Campylobacter infections than previously recognized and help further our understanding of potential sources of human campylobacteriosis.
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Gregory H
authors.initials: GH
authors.lastname: Tyson
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Heather P
authors.initials: HP
authors.lastname: Tate
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Jason
authors.initials: J
authors.lastname: Abbott
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Thu-Thuy
authors.initials: TT
authors.lastname: Tran
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Claudine
authors.initials: C
authors.lastname: Kabera
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Emily
authors.initials: E
authors.lastname: Crarey
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Shenia
authors.initials: S
authors.lastname: Young
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Patrick F
authors.initials: PF
authors.lastname: McDermott
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, 950 College Station Road, Athens, Georgia 30601, USA.
authors.forename: Grisselle
authors.initials: G
authors.lastname: Sprague
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, 950 College Station Road, Athens, Georgia 30601, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Campbell
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, 950 College Station Road, Athens, Georgia 30601, USA.
authors.forename: Oyewole
authors.initials: O
authors.lastname: Adeyemo
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, 950 College Station Road, Athens, Georgia 30601, USA.
authors.forename: Johnette
authors.initials: J
authors.lastname: Browne-Silva
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, 950 College Station Road, Athens, Georgia 30601, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Myers
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, 950 College Station Road, Athens, Georgia 30601, USA.
authors.forename: Sutawee
authors.initials: S
authors.lastname: Thitaram
authors.affiliation: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D002169:Campylobacter Infections; D016123:Campylobacter jejuni / Q000302:isolation & purification; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D008460:Meat; D013552:Swine
keywords: Campylobacter; Food animals; Pulsed-field gel electrophoresis; Source attribution