title: Effects of Boron Supplementation on Peripartum Dairy Cows' Health.
abstract: Although many different dietary studies on the prevention of negative energy balance related diseases are often encountered, this is the first study investigating the effects of boron supplementation on peripartum dairy cows' health in the light of an omics approach. Twenty-eight healthy cows (1 control and 3 experimental groups) were enrolled from 2 months before predicted calving until 2 months after calving. Experimental groups were assigned to receive boron at increasing doses as an oral bolus. Production parameters, biochemical profile, Nuclear Magnetic Resonance based metabolomics profile, and mRNA abundance of gluconeogenic enzymes and lipid oxidation genes were determined. Pivotal knowledge was obtained on boron distribution in the body. Production parameters and mRNA abundance of the genes were not affected by the treatments. Postpartum nonesterified fatty acids, β-hydroxybutyrate, and triglyceride concentrations were significantly decreased in experimentals. The primary differences among groups were in lipid-soluble metabolites. There were significant differences in metabolites including postpartum valine, β-hydroxybutyrate, polyunsaturated fatty acid and citrate, propionate, isobutyrate, choline metabolites (betaine, phosphatidylcholine, and sphingomyelin), and some types of fatty acids and cholesterol in experimentals. Boron appears to be effective in minimizing negative energy balance and improving health of postpartum dairy cows.
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Aleaddin Keykubat Campus, 42250, Selcuklu, Konya, Turkey. abbasoglu@selcuk.edu.tr.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Basoglu
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Selcuk University, Aleaddin Keykubat Campus, 42250, Selcuklu, Konya, Turkey.
authors.forename: Nuri
authors.initials: N
authors.lastname: Baspinar
authors.affiliation: Consorzio Department of Experimental and Clinical Medicine, University of Florence, Largo Brambilla 3, 50134, Florence, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Tenori
authors.affiliation: Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino (FI), Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Vignoli
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Aleaddin Keykubat Campus, 42250, Selcuklu, Konya, Turkey.
authors.forename: Erdem
authors.initials: E
authors.lastname: Gulersoy
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001895:Boron / Q000493:pharmacokinetics / Q000494:pharmacology*; D002417:Cattle; D003612:Dairying; D019587:Dietary Supplements; D004734:Energy Metabolism / Q000187:drug effects; D004798:Enzymes / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007774:Lactation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome / Q000187:drug effects*; D058725:Peripartum Period / Q000187:drug effects*; D011247:Pregnancy; D014018:Tissue Distribution
chemical_list: D004798:Enzymes; D001895:Boron
keywords: Boron; Metabolomics; NMR; Transition cow; mRNA