title: Genome Transfer Prevents Fragmentation and Restores Developmental Potential of Developmentally Compromised Postovulatory Aged Mouse Oocytes.
abstract: Changes in oocyte quality can have great impact on the developmental potential of early embryos. Here we test whether nuclear genome transfer from a developmentally incompetent to a developmentally competent oocyte can restore developmental potential. Using in vitro oocyte aging as a model system we performed nuclear transfer in mouse oocytes at metaphase II or at the first interphase, and observed that development to the blastocyst stage and to term was as efficient as in control embryos. The increased developmental potential is explained primarily by correction of abnormal cytokinesis at anaphase of meiosis and mitosis, by a reduction in chromosome segregation errors, and by normalization of the localization of chromosome passenger complex components survivin and cyclin B1. These observations demonstrate that developmental decline is primarily due to abnormal function of cytoplasmic factors involved in cytokinesis, while the genome remains developmentally fully competent.
authors.affiliation: The New York Stem Cell Foundation Research Institute, New York, NY 10032, USA.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yamada
authors.affiliation: The New York Stem Cell Foundation Research Institute, New York, NY 10032, USA; Department of Pediatrics, Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA. Electronic address: de2220@cumc.columbia.edu.
authors.forename: Dieter
authors.initials: D
authors.lastname: Egli
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D016922:Cellular Senescence / Q000235:genetics; D056744:Cyclin B1 / Q000378:metabolism; D053938:DNA Fragmentation; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro; D016678:Genome; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D008540:Meiosis / Q000235:genetics; D051379:Mice; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D000077022:Survivin
chemical_list: C117749:Birc5 protein, mouse; D056744:Cyclin B1; D051034:Inhibitor of Apoptosis Proteins; D012097:Repressor Proteins; D000077022:Survivin
keywords: fragmentation; genome exchange; mouse; oocyte aging