title: Stellera chamaejasme and its constituents induce cutaneous wound healing and anti-inflammatory activities.
abstract: Stellera chamaejasme L. (Thymelaeaceae) is a perennial herb that is widely used in traditional Chinese medicine to treat tumours, tuberculosis and psoriasis. S. chamaejasme extract (SCE) possesses anti-inflammatory, analgesic and wound healing activities; however, the effect of S. chamaejasme and its active compounds on cutaneous wound healing has not been investigated. We assessed full-thickness wounds of Sprague-Dawley (SD) rats and topically applied SCE for 2 weeks. In vitro studies were performed using HaCaT keratinocytes, Hs68 dermal fibroblasts and RAW 264.7 macrophages to determine cell viability (MTT assay), cell migration, collagen expression, nitric oxide (NO) production, prostaglandin E2(PGE2) production, inflammatory cytokine expression and β-catenin activation. In vivo, wound size was reduced and epithelisation was improved in SCE-treated SD rats. In vitro, SCE and its active compounds induced keratinocyte migration by regulating the β-catenin, extracellular signal-regulated kinase and Akt signalling pathways. Furthermore, SCE and its active compounds increased mRNA expression of type I and III collagen in Hs68 fibroblasts. SCE and chamechromone inhibited NO and PGE2release and mRNA expression of inflammatory mediators in RAW 264.7 macrophages. SCE enhances the motility of HaCaT keratinocytes and improves cutaneous wound healing in SD rats.
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.|Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
authors.forename: Myungsuk
authors.initials: M
authors.lastname: Kim
authors.affiliation: Systems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
authors.forename: Hee Ju
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.|Department of Biological Chemistry, Korea, University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Randy
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.|Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
authors.forename: Ji Ho
authors.initials: JH
authors.lastname: Yun
authors.affiliation: Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
authors.forename: Sang-Rok
authors.initials: SR
authors.lastname: Oh
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.|Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
authors.forename: Chu Won
authors.initials: CW
authors.lastname: Nho
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D002853:Chromatography, Liquid; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D015394:Molecular Structure; D051058:Oncogene Protein v-akt / Q000378:metabolism; D064209:Phytochemicals / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D012867:Skin / Q000378:metabolism / Q000473:pathology; D029645:Thymelaeaceae / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D064209:Phytochemicals; D010936:Plant Extracts; D003094:Collagen; D051058:Oncogene Protein v-akt; D048049:Extracellular Signal-Regulated MAP Kinases