title: Comparison of nanoparticular hydroxyapatite pastes of different particle content and size in a novel scapula defect model.
abstract: Nanocrystalline hydroxyapatite (HA) has good biocompatibility and the potential to support bone formation. It represents a promising alternative to autologous bone grafting, which is considered the current gold standard for the treatment of low weight bearing bone defects. The purpose of this study was to compare three bone substitute pastes of different HA content and particle size with autologous bone and empty defects, at two time points (6 and 12 months) in an ovine scapula drillhole model using micro-CT, histology and histomorphometry evaluation. The nHA-LC (38% HA content) paste supported bone formation with a high defect bridging-rate. Compared to nHA-LC, Ostim®(35% HA content) showed less and smaller particle agglomerates but also a reduced defect bridging-rate due to its fast degradation The highly concentrated nHA-HC paste (48% HA content) formed oversized particle agglomerates which supported the defect bridging but left little space for bone formation in the defect site. Interestingly, the gold standard treatment of the defect site with autologous bone tissue did not improve bone formation or defect bridging compared to the empty control. We concluded that the material resorption and bone formation was highly impacted by the particle-specific agglomeration behaviour in this study.
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Veronika
authors.initials: V
authors.lastname: Hruschka
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria.|Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Department of Oral Surgery, Medical University of Vienna, Vienna, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Tangl
authors.affiliation: Department of Materials Science and Engineering, The University of Sheffield, Sheffield, United Kingdom.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Ryabenkova
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.|Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Department of Oral Surgery, Medical University of Vienna, Vienna, Austria.
authors.forename: Patrick
authors.initials: P
authors.lastname: Heimel
authors.affiliation: Research Center for Medical Technology and Biotechnology, Bad Langensalza, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Barnewitz
authors.affiliation: Department of Materials Science and Engineering, The University of Sheffield, Sheffield, United Kingdom.
authors.forename: Günter
authors.initials: G
authors.lastname: Möbus
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Keibl
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: James
authors.initials: J
authors.lastname: Ferguson
authors.affiliation: Fluidinova S.A., Moreira da Maia, Portugal.
authors.forename: Paulo
authors.initials: P
authors.lastname: Quadros
authors.affiliation: The School of Clinical Dentistry, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Cheryl
authors.initials: C
authors.lastname: Miller
authors.affiliation: Ceramisys Ltd., Sheffield, United Kingdom.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Goodchild
authors.affiliation: Ceramisys Ltd., Sheffield, United Kingdom.
authors.forename: Wayne
authors.initials: W
authors.lastname: Austin
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Nau
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D016025:Bone Transplantation / Q000379:methods; D004195:Disease Models, Animal; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D053758:Nanoparticles / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects; D010316:Particle Size; D012540:Scapula / Q000000981:diagnostic imaging / Q000187:drug effects / Q000293:injuries; D012756:Sheep; D000072836:Surgical Wound / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D014182:Transplantation, Autologous; D055114:X-Ray Microtomography
chemical_list: D001843:Bone Cements; D018786:Bone Substitutes; D017886:Durapatite