title: [Phosphatidylserine externalization and functional-morphological impairment of sperm in men with long barren marriage].
abstract: OBJECTIVE
  To identify the relationship between phosphatidylserine externalization, as an early marker of apoptosis, and functional and morphological sperm impairment in infertile men to subsequently evaluate the effect of apoptosis on sperm fertility.
  
  
  METHODS
  Ejaculates of 18 fertile and 78 subfertile men were examined. Phosphatidylserine externalization was detected by staining the sperm with fluorochrome conjugated Annexin V (AnV-FITC) and propidium iodide using fluorescence microscopy.
  
  
  RESULTS
  and conclusions: Ejaculates of fertile and subfertile men differed in the percentage of annexin-V-positive sperm. The correlation of (AnV+/PI+) - sperm of subfertile men with sperm concentration, motility and defects of sperm morphology shows the adverse effects of apoptosis on sperm quality and suggests that phosphatidylserine externalization is a factor for reducing sperm fertility.
authors.affiliation: Department of Chemistry, Astrakhan State Medical University of Minzdrav of Russia.|Department of Natural Sciences, Volga State University of Water Transport of the Caspian Institute of Maritime and River Transport, Astrakhan.
authors.forename: M V
authors.initials: MV
authors.lastname: Ploskonos
mesh_terms: D000328:Adult; D017209:Apoptosis; D016022:Case-Control Studies; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008393:Marriage; D010718:Phosphatidylserines / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: D010718:Phosphatidylserines
keywords: apoptosis; fertility; phosphatidylserine; sperm