title: Analysis of PAMP-Triggered ROS Burst in Plant Immunity.
abstract: The plant perception of pathogen-associated molecular patterns triggers a plethora of cellular immune responses. One of these responses is a rapid and transient burst of reactive oxygen species (ROS) mediated by plasma membrane-localized NADPH oxidases. The ROS burst requires a functional receptor complex and the contribution of several additional regulatory components. In laboratory conditions, the ROS burst can be detected a few minutes after the treatment with an immunogenic microbial elicitor. For these reasons, the elicitor-triggered ROS burst has been often exploited as readout to probe the contribution of plant components to early immune responses. Here, we describe a detailed protocol for the measurement of elicitor-triggered ROS burst in a simple, fast, and easy manner.
authors.affiliation: CAS Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 201602, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Sang
authors.affiliation: CAS Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 201602, China. alberto.macho@sibs.ac.cn.
authors.forename: Alberto P
authors.initials: AP
authors.lastname: Macho
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005408:Flagellin / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D019255:NADPH Oxidases / Q000378:metabolism; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000378:metabolism; D057865:Plant Immunity / Q000187:drug effects; D010944:Plants / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism
chemical_list: D000069452:Pathogen-Associated Molecular Pattern Molecules; D017382:Reactive Oxygen Species; D005408:Flagellin; D019255:NADPH Oxidases
keywords: Immunity; PAMP; ROS; Reactive oxygen species; flg22