title: Proteomic Applications in the Study of Human Mesenchymal Stem Cells.
abstract: Mesenchymal stem cells (MSCs) are undifferentiated cells with an unlimited capacity for self-renewal and able to differentiate towards specific lineages under appropriate conditions. MSCs are,a priori, a good target for cell therapy and clinical trials as an alternative to embryonic stem cells, avoiding ethical problems and the chance for malignant transformation in the host. However, regarding MSCs, several biological implications must be solved before their application in cell therapy, such as safeex vivoexpansion and manipulation to obtain an extensive cell quantity amplification number for use in the host without risk accumulation of genetic and epigenetic abnormalities. Cell surface markers for direct characterization of MSCs remain unknown, and the precise molecular mechanisms whereby growth factors stimulate their differentiation are still missing. In the last decade, quantitative proteomics has emerged as a promising set of techniques to address these questions, the answers to which will determine whether MSCs retain their potential for use in cell therapy. Proteomics provides tools to globally analyze cellular activity at the protein level. This proteomic profiling allows the elucidation of connections between broad cellular pathways and molecules that were previously impossible to determine using only traditional biochemical analysis. However; thus far, the results obtained must be orthogonally validated with other approaches. This review will focus on how these techniques have been applied in the evaluation of MSCs for their future applications in safe therapies.
authors.affiliation: Rheumatology Division, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña, A Coruña 15006, Spain. jesus.mateos.martin@sergas.es.
authors.forename: Jesús
authors.initials: J
authors.lastname: Mateos
authors.affiliation: CIBER-BBN, INIBIC-Hospital Universitario A Coruña, A Coruña 15006, Spain. pablofpernas@gmail.com.|Department of Medicine, University of A Coruña, A Coruña 15006, Spain. pablofpernas@gmail.com.
authors.forename: Pablo Fernández
authors.initials: PF
authors.lastname: Pernas
authors.affiliation: CIBER-BBN, INIBIC-Hospital Universitario A Coruña, A Coruña 15006, Spain. juanlaru_15@hotmail.com.|Department of Medicine, University of A Coruña, A Coruña 15006, Spain. juanlaru_15@hotmail.com.
authors.forename: Juan Fafián
authors.initials: JF
authors.lastname: Labora
authors.affiliation: Rheumatology Division, ProteoRed/ISCIII, INIBIC-Hospital Universitario A Coruña, A Coruña 15006, Spain. fblagar@sergas.es.|CIBER-BBN, INIBIC-Hospital Universitario A Coruña, A Coruña 15006, Spain. fblagar@sergas.es.|Department of Medicine, University of Santiago de Compostela, Santiago de Compostela 15782, Spain. fblagar@sergas.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Blanco
authors.affiliation: CIBER-BBN, INIBIC-Hospital Universitario A Coruña, A Coruña 15006, Spain. maria.arufe@udc.es.|Department of Medicine, University of A Coruña, A Coruña 15006, Spain. maria.arufe@udc.es.
authors.forename: María Del Carmen
authors.initials: MD
authors.lastname: Arufe
keywords: characterization; differentiation; mesenchymal stem cell; proteomic analysis