title: The complete genome sequence of Bacillus velezensis 9912D reveals its biocontrol mechanism as a novel commercial biological fungicide agent.
abstract: A Bacillus sp. 9912 mutant, 9912D, was approved as a new biological fungicide agent by the Ministry of Agriculture of the People's Republic of China in 2016 owing to its excellent inhibitory effect on various plant pathogens and being environment-friendly. Here, we present the genome of 9912D with a circular chromosome having 4436 coding DNA sequences (CDSs), and a circular plasmid encoding 59 CDSs. This strain was finally designated as Bacillus velezensis based on phylogenomic analyses. Genome analysis revealed a total of 19 candidate gene clusters involved in secondary metabolite biosynthesis, including potential new type II lantibiotics. The absence of fengycin biosynthetic gene cluster is noteworthy. Our data offer insights into the genetic, biological and physiological characteristics of this strain and aid in deeper understanding of its biocontrol mechanism.
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, PR China.
authors.forename: Hua-Qi
authors.initials: HQ
authors.lastname: Pan
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
authors.forename: Qing-Lian
authors.initials: QL
authors.lastname: Li
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, PR China. Electronic address: hujc@iae.ac.cn.
authors.forename: Jiang-Chun
authors.initials: JC
authors.lastname: Hu
mesh_terms: D001407:Bacillus / Q000235:genetics*; D002874:Chromosome Mapping; D059646:Genome Size; D016680:Genome, Bacterial; D005810:Multigene Family; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D064210:Secondary Metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: Bacillus methylotrophicus; Bacillus velezensis; Biocontrol; Genome; Secondary metabolite