title: Antibacterial Performance of a PCL-PDMAEMA Blend Nanofiber-Based Scaffold Enhanced with Immobilized Silver Nanoparticles.
abstract: In the present study, nanofiber meshes (NFs), composed of polycaprolactone and poly[(2-dimethylamino)ethyl methacrylate] at 80/20 and 50/50 PCL/PDMAEMA blend ratios, were obtained through electrospinning. Silver nanoparticles (AgNPs) formed in situ were then immobilized on NF surfaces through adsorption processes at different pHs. It was possible to observe that the amount of NF-AgNPs can be tuned by changing the pH of AgNPs immobilization and the PCL/PDMAEMA ratio in the blend. The neat NF and NF-AgNPs were characterized with respect to their morphology and mechanical properties. The effects of AgNPs on the antibacterial activities and cytotoxicity of meshes were also evaluated. The antibacterial performance of such NF was improved by the presence of AgNPs. The NF-AgNPs presented good antibacterial effect against S. aureus and partial toxicity against E. coli and P. aeruginosa. Also, compared with neat PCL/PDMAEMA the NF-AgNPs presented lower cytotoxicity against VERO cells, showing their potential for applications in tissue engineering for different types of cell growth.
authors.forename: Fernanda G
authors.initials: FG
authors.lastname: Santos
authors.forename: Letícia C
authors.initials: LC
authors.lastname: Bonkovoski
authors.forename: Francielle P
authors.initials: FP
authors.lastname: Garcia
authors.forename: Thelma S P
authors.initials: TS
authors.lastname: Cellet
authors.affiliation: Pontifícia Universidade Católica do Paraná (PUCPR), 80215-901 Curitiba, PR, Brazil.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Witt
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.affiliation: Universidade Tecnológica Federal do Paraná (UTFPR-LD), PPGCEM, 86036-370 Londrina, PR, Brazil.|Programa de Pós-graduação em Biotecnologia Aplicada a Agricultura, Universidade Paranaense (UNIPAR) , 87502-210, Umuarama, PR, Brazil.
authors.forename: Edvani C
authors.identifier: http://orcid.org/0000-0001-6685-1519
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D002522:Chlorocebus aethiops; D004926:Escherichia coli; D053768:Metal Nanoparticles / Q000737:chemistry*; D008689:Methacrylates; D057139:Nanofibers; D009757:Nylons; D012834:Silver; D013211:Staphylococcus aureus; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D008689:Methacrylates; D009757:Nylons; C407037:poly(2-(dimethylamino)ethyl methacrylate); D012834:Silver
keywords: PCL; PDMAEMA; electrospinning; nanofibers; silver nanoparticles