title: Adherence of staphylococcus aureus to catheter tubing inhibition by quaternary ammonium compounds.
abstract: BACKGROUND
  S. aureusis a Gram positive bacterium which is responsible for a wide range of infections. This pathogen has also the ability to adhere to biotic or abiotic surface such as central venous catheter (CVC) and to produce a biofilm. The aim of this study was to evaluate the effect of hexadecyltrimethyl ammonium bromide (HTAB) and Hexadecylbetainate chloride (HBC) onStaphylococcus aureusadherence to the catheter tubing and on bacteria growth.
  
  
  METHODS
  Broth microdilution method was used to determine the Minimal Inhibitory Concentration (MIC). The detection of slime production was done by Congo Red Agar method, and the adherence of bacteria to the catheter tubing was evaluated by the enumeration of bacteria on plate counts.
  
  
  RESULTS
  The results of this study showed that the MICs of HTAB were ranged from 0.125 to 0.5 µg/mL, and those of HBC fluctuated between 2 to 8 µg/mL. HTAB and HBC inhibited bacteria adhesion on the surface of the catheter tubing.
  
  
  CONCLUSIONS
  This study showed that HTAB and HBC can prevent the adherence ofS. aureusstrains to the surface of catheter tubing, suggesting that they could be used to prevent the risk of catheter related bloodstream infections.
authors.affiliation: Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo.
authors.forename: Jean-Marie Liesse
authors.initials: JL
authors.lastname: Iyamba
authors.affiliation: Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo.
authors.forename: Daniel Tassa
authors.initials: DT
authors.lastname: Okombe
authors.affiliation: Laboratory of Biochemistry, Faculty of Medicine, University of Kinshasa, Democratic Republic of Congo.
authors.forename: Francis Nsimba
authors.initials: FN
authors.lastname: Zakanda
authors.affiliation: Laboratory of Instrumental and Bioelectrochemical analysis, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo.
authors.forename: Trésor Kimbeni
authors.initials: TK
authors.lastname: Malongo
authors.affiliation: Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo.
authors.forename: Joseph Welo
authors.initials: JW
authors.lastname: Unya
authors.affiliation: Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo.
authors.forename: Cyprien Mbundu
authors.initials: CM
authors.lastname: Lukukula
authors.affiliation: Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo.
authors.forename: Ntondo Za Balega Takaisi
authors.initials: NZ
authors.lastname: Kikuni
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology; D001622:Betaine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D055499:Catheter-Related Infections / Q000382:microbiology / Q000517:prevention & control*; D057785:Catheters / Q000382:microbiology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000891:Anti-Infective Agents, Local; D002593:Cetrimonium Compounds; C573900:hexadecylbetainate chloride; D001622:Betaine; D000077286:Cetrimonium
keywords: Staphylococcus aureus; betaine ester; biofilm; catheter; quaternary ammonium