title: Expression of Surface Molecules in Human Mesenchymal Stromal Cells Co-Cultured with Nucleated Umbilical Cord Blood Cells.
abstract: We studied the expression of different classes of surface molecules (CD13, CD29, CD40, CD44, CD54, CD71, CD73, CD80, CD86, CD90, CD105, CD106, CD146, HLA-I, and HLA-DR) in mesenchymal stromal cells from human umbilical cord and bone marrow during co-culturing with nucleated umbilical cord blood cells. Expression of the majority of surface markers in both types of mesenchymal stromal cells was stable and did not depend on the presence of the blood cells. Significant differences were found only for cell adhesion molecules CD54 (ICAM-1) and CD106 (VCAM-1) responsible for direct cell-cell contacts with leukocytes and only for bone marrow derived cells.
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia. romanov@cardio.ru.|CryoCenter Cord Blood Bank, Moscow, Russia. romanov@cardio.ru.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Romanov
authors.affiliation: CryoCenter Cord Blood Bank, Moscow, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Balashova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N E
authors.initials: NE
authors.lastname: Volgina
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Kabaeva
authors.affiliation: CryoCenter Cord Blood Bank, Moscow, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Dugina
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D061251:Primary Cell Culture; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1
keywords: co-culture; differentiation clusters; flow cytometry; mesenchymal stromal cells; umbilical cord blood