title: Increased Serum Antibody Titer against HPV-16 Antigen in Patients with Behçet's Disease.
abstract: Quadrivalent human papillomavirus (HPV) vaccine has been reported to be significantly associated with Behçet's disease (BD). However, no reports have described HPV infection as a possible cause for the development of BD. The objective of this study was to evaluate whether anti-HPV immunoglobulin G (IgG) antibody titer is increased in BD. Serum samples from 93 Korean BD patients, who fulfilled the diagnostic criteria of the International Study Group for BD, were used in an enzyme-linked immunosorbent assay (ELISA). The clinical activity of BD was evaluated at the time of blood sampling. HPV-16 L1 virus-like particle (VLP) antigen was used in this study for the ELISA. Patients with BD had significantly higher antibody titers against HPV-16 (optical density [OD], 0.210-3.675; mean 0.992) than that of healthy controls (OD, 0.248-0.762; mean 0.517; P < 0.001). Using a receiver operating characteristic (ROC) analysis, a cut-off value of 0.578 OD for the anti-HPV antibody titer was determined that differentiated BD patients from healthy controls. When we compared the clinical features of BD between the 2 groups, articular involvement of BD was more likely in patients with an anti-HPV-16 antibody titer < 0.578 OD (P = 0.035). In addition, patients with an anti-HPV-16 antibody titer < 0.578 were significantly younger than those with a titer ≥ 0.578 OD. HPV itself may be a possible extrinsic triggering infectious agent causing the development of BD.
authors.affiliation: Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Kyu Yeun
authors.identifier: http://orcid.org/0000-0002-8035-4436
authors.initials: KY
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Do Young
authors.identifier: http://orcid.org/0000-0002-0194-9854
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jimyung
authors.identifier: http://orcid.org/0000-0003-3416-9990
authors.initials: J
authors.lastname: Seo
authors.affiliation: Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Yuri
authors.identifier: http://orcid.org/0000-0002-5298-6102
authors.initials: Y
authors.lastname: Ahn
authors.affiliation: Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea. DSKIM6634@yuhs.ac.
authors.forename: Dong Soo
authors.identifier: http://orcid.org/0000-0002-3270-506X
authors.initials: DS
authors.lastname: Kim
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D019540:Area Under Curve; D001528:Behcet Syndrome / Q000150:complications / Q000175:diagnosis*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D052162:Human papillomavirus 16 / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology; D012372:ROC Curve
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G
keywords: Behçet's Disease; Enzyme-Linked Immunosorbent Assay; Human Papilloma Virus; IgG Antibody