title: Scalable, Lightweight, Integrated and Quick-to-Assemble (SLIQ) Hyperdrives for Functional Circuit Dissection.
abstract: Independently adjustable multielectrode arrays are routinely used to interrogate neuronal circuit function, enabling chronicin vivomonitoring of neuronal ensembles in freely behaving animals at a single-cell, single spike resolution. Despite the importance of this approach, its widespread use is limited by highly specialized design and fabrication methods. To address this, we have developed a Scalable, Lightweight, Integrated and Quick-to-assemble multielectrode array platform. This platform additionally integrates optical fibers with independently adjustable electrodes to allow simultaneous single unit recordings and circuit-specific optogenetic targeting and/or manipulation. In current designs, the fully assembled platforms are scalable from 2 to 32 microdrives, and yet range 1-3 g, light enough for small animals. Here, we describe the design process starting from intent in computer-aided design, parameter testing through finite element analysis and experimental means, and implementation of various applications across mice and rats. Combined, our methods may expand the utility of multielectrode recordings and their continued integration with other tools enabling functional dissection of intact neural circuits.
authors.affiliation: Department of Neuroscience and Physiology, New York University Neuroscience Institute, New York University Langone Medical Center, New York NY, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Neuroscience and Physiology, New York University Neuroscience Institute, New York University Langone Medical Center, New York NY, USA.
authors.forename: Stefan N
authors.initials: SN
authors.lastname: Oline
authors.affiliation: Department of Neuroscience and Physiology, New York University Neuroscience Institute, New York University Langone Medical Center, New York NY, USA.
authors.forename: Justin C
authors.initials: JC
authors.lastname: Kirk
authors.affiliation: Department of Neuroscience and Physiology, New York University Neuroscience Institute, New York University Langone Medical Center, New York NY, USA.
authors.forename: Lukas Ian
authors.initials: LI
authors.lastname: Schmitt
authors.affiliation: Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge MA, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Komorowski
authors.affiliation: Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa Lisbon, Portugal.
authors.forename: Miguel
authors.initials: M
authors.lastname: Remondes
authors.affiliation: Department of Neuroscience and Physiology, New York University Neuroscience Institute, New York University Langone Medical Center, New YorkNY, USA; Center for Neural Science, New York University, New YorkNY, USA.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Halassa
mesh_terms: D000818:Animals; D017076:Computer-Aided Design; D055724:Electrophysiological Phenomena / Q000502:physiology*; D004594:Electrophysiology / Q000295:instrumentation*; D004867:Equipment Design; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000295:instrumentation*; D012377:Rodentia
keywords: adjustable electrode; electrophysiology; microdrives; multi-electrode; single unit recording