title: Helicobacter pyloriCagA induced interleukin-8 secretion in gastric epithelial cells.
abstract: OBJECTIVE
  Since, contradictory data have been reported about the effect of diverse variants ofH. pylorivirulence factors on IL-8 induction, we aimed to analyze the effect of this diversity on levels of IL-8 secretion in AGS cell line.
  
  
  BACKGROUND
  Helicobacter pyloricolonizes the human stomach and induces the activation of inflammatory cytokines, including interleukin (IL)-8, in the gastric mucosa. This induction promotes neutrophil and monocyte recruitment that causes gastric tissue damage.
  
  
  METHODS
  To determine whether different strains ofH. pyloriand their CagA variants have possible roles on IL-8 induction, polarized AGS cell line was infected with CagA+H. pyloristrains carrying different EPIYA motifs (ABCCC and ABC) and CagA- strain for 24 hours. Difference in stimulation of IL-8 was measured by ELISA.
  
  
  RESULTS
  IL-8 secretion was elevated in the treated cells with CagA encoding strains compared with the negative one. Furthermore, a noticeably increased level of IL-8 induction was measured bythe CagA-EPIYA type ABCCC encoding strain in compare to that carried EPIYA type ABC.
  
  
  CONCLUSIONS
  Results of this study provide new evidence about different effects ofH. pyloristrains and possible roles of their CagA variants on IL-8 induction. It seems that not only carriage ofcagAand its expression, but also diversity in EPIYA motif be involved in IL-8 induction in the gastric epithelial cells.
authors.affiliation: Faculty of Paramedical Sciences, Department of Basic Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Fazeli
authors.affiliation: Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Alebouyeh
authors.affiliation: Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaei Tavirani
authors.affiliation: Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Azimirad
authors.affiliation: Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Yadegar
keywords: CagA variants; Cytotoxin-associated gene A; Helicobacter pylori; Interleukin-8