title: Spirosoma luteolum sp. nov. isolated from water.
abstract: A novel Gram-negative and rod-shaped bacterial strain, designated as 16F6ET, was isolated from a water sample. Cells were yellowish in color and catalase- and oxidase-positive. The strain grew at 10-37°C (optimum at 25°C) but not at 4 and 42°C, and pH 5-7 (optimum at pH 7). It showed moderate resistance to gamma-ray irradiation. Comparative phylogenetic analysis showed that strain 16F6ETbelonged to the family Cytophagaceae of the class Cytophagia. Furthermore, this isolate showed relatively low 16S rRNA gene sequence similarities (90.7-93.1%) to the members of the genus Spirosoma. The major fatty acids were summed feature 3 (C16:1ω7c/C16:1ω6c), C16:1ω5c, C16:0N alcohol, and C16:0. The polar lipid profile indicated presence of phosphatidylethanolamine, unknown aminophospholipids, an unknown amino lipid, unknown phospholipids, and unknown polar lipids. The predominant isoprenoid quinone was MK-7. The genomic DNA G+C content of strain 16F6ETwas 56.5 mol%. Phenotypic, phylogenetic, and chemotaxonomic properties indicated that isolate 16F6ETrepresents a novel species within the genus Spirosoma, for which the name Spirosoma luteolum sp. nov. is proposed. The type strain is 16F6ET(=KCTC 52199T=JCM 31411T).
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Park
authors.affiliation: School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Young-Je
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Department of Bio and Environmental Technology, Seoul Women's University, Seoul, 01797, Republic of Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.|Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
mesh_terms: D001482:Base Composition; D002374:Catalase / Q000032:analysis; D016000:Cluster Analysis; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D010088:Oxidoreductases / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase
keywords: Spirosoma; polyphasic taxonomy; water