title: Relationships between inflammation- and immunity-related transcript abundance in the rumen and jejunum of beef steers with divergent average daily gain.
abstract: The bovine rumen papillae are in contact with a wide array of microorganisms and the metabolites they produce, which may activate an inflammatory and/or immune response. Cytokines, chemokines and their receptor genes were tested for differential expression in the rumen and jejunum of beef steers with greater and lesser average daily body weight gain (ADG) near the average daily dry matter intake (DMI) for the population. Angus-sired steers (n = 16) were used to represent the greater (ADG = 2.2 ± 0.07 kg/day; DMI = 10.1 ± 0.05 kg/day) and lesser (ADG = 1.7 ± 0.05 kg/day; DMI = 10.1 ± 0.05 kg/day) ADG groups with eight steers each. Rumen epithelium and jejunum mucosal samples were collected at slaughter, and gene expression was evaluated using a commercially available qRT-PCR array containing 84 genes representing chemokines, cytokines and their receptors. None of the genes on the array were differentially expressed in the jejunum of the steers with greater vs. lesser ADG. However, in the rumen, two chemokine genes (CCL11, CXCL5) and one receptor gene (IL10RA) were detected as differentially expressed (P < 0.05). The genes IL1A, BMP2, CXCL12 and TNFSF13 also displayed trends for differential expression (P < 0.10). All of the genes identified were lower in transcript abundance in the greater ADG animals. Thus, greater ADG steers have a lesser inflammatory response in the rumen papillae, which may lead to a more efficient use of nutrients.
authors.affiliation: USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, 68933-0166, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Reynolds
authors.affiliation: USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, 68933-0166, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Foote
authors.affiliation: USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, 68933-0166, USA.
authors.forename: H C
authors.initials: HC
authors.lastname: Freetly
authors.affiliation: USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, 68933-0166, USA.
authors.forename: W T
authors.initials: WT
authors.lastname: Oliver
authors.affiliation: USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, 68933-0166, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Lindholm-Perry
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D015870:Gene Expression; D007107:Immune System; D007249:Inflammation / Q000235:genetics*; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D012417:Rumen / Q000378:metabolism*; D015430:Weight Gain
keywords: cattle; feed efficiency; gene expression; immune response; inflammation