title: Palmatine inhibits TRIF-dependent NF-κB pathway against inflammation induced by LPS in goat endometrial epithelial cells.
abstract: Palmatine, a natural pharmaceutical drug, possesses many biological activities. But its clinical application is rarely reported in the veterinary medicine. The aim of this study was to investigate the anti-inflammatory effects of palmatine on lipopolysaccharide (LPS)-induced inflammation in goat endometrial epithelial cells (gEECs), and the possible molecular mechanisms. Palmatine cell toxicity was determined by MTT assay, and the production of inflammatory cytokine in the cultured medium was measured with ELISA, qRT-PCR and Western blotting. Our results showed that palmatine treatment inhibited the release of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, nitric oxide (NO), matrix metalloproteinase (MMP)-9 and MMP-2. Furthermore, palmatine enhanced the secretion of prostaglandins E2(PGE2) and IL-10. Palmatine significantly down-regulated the expression of Toll-like receptor 4 (TLR4), cluster of differentiation 14 (CD14), Toll/interleukin 1 receptor (TIR)-domain-containing adaptor protein inducing interferon-β (TICAM, TRIF) and nuclear factor-κB (NF-κB) in LPS stimulated gEECs, but did not alter the production of MyD88. In conclusion, palmatine inhibits TRIF-dependent NF-κB pathway to reduce LPS-induced inflammatory responses in goat endometrial epithelial cells.
authors.affiliation: Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. Electronic address: m15624955865@163.com.
authors.forename: Baoqi
authors.initials: B
authors.lastname: Yan
authors.affiliation: Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wang
authors.affiliation: Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
authors.forename: Shuwei
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Production and Population Health, Royal Veterinary College, North Mymms, Hertfordshire, United Kingdom.
authors.forename: Zhangrui
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Na
authors.affiliation: Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Sang
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Hongzao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. Electronic address: zhangshidong@caas.cn.
authors.forename: Shidong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. Electronic address: yanzuoting@caas.cn.
authors.forename: Zuoting
authors.initials: Z
authors.lastname: Yan
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D004717:Endometrium / Q000473:pathology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006041:Goats; D007249:Inflammation / Q000188:drug therapy*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000893:Anti-Inflammatory Agents; D001600:Berberine Alkaloids; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; C005413:palmatine
keywords: LPS; NF-κB pathway; Palmatine; TLR(4); anti-inflammatory mechanism