title: Long-Lived Protein Degradation During Autophagy.
abstract: Macroautophagy, a major lysosomal degradative pathway for cytoplasmic components, is a process that can be stimulated in response to many stressful situations including cancer treatment. The central autophagic organelle is the autophagosome, a double-membrane-bound vacuole that sequesters cytoplasmic material. The ultimate destiny of the autophagosome is fusion with the lysosomal compartment, where cargo, including proteins, is degraded. Here, we report a method to measure the lysosomal degradation of long-lived proteins along the autophagic pathway.
authors.affiliation: Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Université Paris Descartes-Sorbonne Paris Cité, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Dupont
authors.affiliation: Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Université Paris Descartes-Sorbonne Paris Cité, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Leroy
authors.affiliation: Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Université Paris Descartes-Sorbonne Paris Cité, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Hamaï
authors.affiliation: Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Université Paris Descartes-Sorbonne Paris Cité, Paris, France. Electronic address: patrice.codogno@inserm.fr.
authors.forename: P
authors.initials: P
authors.lastname: Codogno
mesh_terms: D000818:Animals; D001343:Autophagy; D001671:Biochemistry / Q000379:methods; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D059748:Proteolysis
chemical_list: D011506:Proteins
keywords: Autophagy related; Lysosome; Macroautophagy; Proteolysis