title: Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis.
abstract: β-carotenoid hydroxylase (CHYB) is an important rate-limiting enzyme in the biosynthesis of plant carotenoid. In this study, chyb1 and chyb2, two gene families in Dunaliella viridis were obtained by RNA-seq. The fragment of promoters of CHYB family genes, 1 080 bp for chyb1 (GenBank No. KY012338) and 1 155 bp for chyb2 (GenBank No. KY012339) were cloned by the Genome Walking Technology, respectively. Cis-acting elements of two promoters were analyzed by Plantcare soft. The results show that the chyb1 gene promoter contains more cis-acting elements in responses to abiotic stresses, such as methyl jasmonate, arachidonic acid, acetylsalicylic acid, and so on. On the other hand, the chyb2 promoter contains more cis-acting elements in response to light stress. qRT-PCR results show that the mRNA expression levels of CHYBs are modulated by their promoters, and different CHYB gene families response to distinct stresses.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Shuai-qi
authors.initials: SQ
authors.lastname: Zhu
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Yi-fu
authors.initials: YF
authors.lastname: Gong
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yu
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: He-yu
authors.initials: HY
authors.lastname: Wang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Yan
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis*; D000460:Chlorophyta / Q000201:enzymology* / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological
chemical_list: D002338:Carotenoids; D006899:Mixed Function Oxygenases; C079232:beta-carotene hydroxylase