title: Regional Specializations of the PAZ Proteomes Derived from Mouse Hippocampus, Olfactory Bulb and Cerebellum.
abstract: Neurotransmitter release as well as structural and functional dynamics at the presynaptic active zone (PAZ) comprising synaptic vesicles attached to the presynaptic plasma membrane are mediated and controlled by its proteinaceous components. Here we describe a novel experimental design to immunopurify the native PAZ-complex from individual mouse brain regions such as olfactory bulb, hippocampus, and cerebellum with high purity that is essential for comparing their proteome composition. Interestingly, quantitative immunodetection demonstrates significant differences in the abundance of prominent calcium-dependent PAZ constituents. Furthermore, we characterized the proteomes of the immunoisolated PAZ derived from the three brain regions by mass spectrometry. The proteomes of the release sites from the respective regions exhibited remarkable differences in the abundance of a large variety of PAZ constituents involved in various functional aspects of the release sites such as calcium homeostasis, synaptic plasticity and neurogenesis. On the one hand, our data support an identical core architecture of the PAZ for all brain regions and, on the other hand, demonstrate that the proteinaceous composition of their presynaptic active zones vary, suggesting that changes in abundance of individual proteins strengthen the ability of the release sites to adapt to specific functional requirements.
authors.affiliation: These authors contributed equally to this work.. Weingarten@bio.uni-frankfurt.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Weingarten
authors.affiliation: These authors contributed equally to this work.. Lassek@bio.uni-frankfurt.de.
authors.forename: Melanie
authors.initials: M
authors.lastname: Laßek
authors.affiliation: Department Molecular and Cellular Neurobiology, Institute for Cell Biology and Neuroscience, Goethe University Frankfurt am Main, Max-von-Laue-Straße 13, 60438 Frankfurt am Main, Germany. Benjamin.mueller@gmx.de.
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Mueller
authors.affiliation: Department Molecular and Cellular Neurobiology, Institute for Cell Biology and Neuroscience, Goethe University Frankfurt am Main, Max-von-Laue-Straße 13, 60438 Frankfurt am Main, Germany. rohmer@pharmchem.uni-frankfurt.de.
authors.forename: Marion
authors.initials: M
authors.lastname: Rohmer
authors.affiliation: Institute of Pharmaceutical Chemistry, Goethe University Frankfurt am Main, Max-von-Laue-Straße 9; 60438 Frankfurt am Main, Germany. dbaeumlisberger@sunchrom.de.
authors.forename: Dominic
authors.initials: D
authors.lastname: Baeumlisberger
authors.affiliation: These authors contributed equally to this work.. bbeckert@stud.uni-frankfurt.de.
authors.forename: Benedikt
authors.initials: B
authors.lastname: Beckert
authors.affiliation: These authors contributed equally to this work.. ade@stud.uni-frankfurt.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Ade
authors.affiliation: SunChrom Wissenschaftliche Geräte GmbH, Industriestraße 27, 61381 Friedrichsdorf, Germany. patricia.gogesch@pei.de.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gogesch
authors.affiliation: These authors contributed equally to this work.. Acker-palmer@bio.uni-frankfurt.de.
authors.forename: Amparo
authors.initials: A
authors.lastname: Acker-Palmer
authors.affiliation: Department Molecular and Cellular Neurobiology, Institute for Cell Biology and Neuroscience, Goethe University Frankfurt am Main, Max-von-Laue-Straße 13, 60438 Frankfurt am Main, Germany. karas@pharmchem.uni-frankfurt.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Karas
authors.affiliation: These authors contributed equally to this work.. Volknandt@bio.uni-frankfurt.de.
authors.forename: Walter
authors.initials: W
authors.lastname: Volknandt
keywords: cerebellum; hippocampus; olfactory bulb; presynaptic active zone; proteome