title: Acellular Mouse Kidney ECM can be Used as a Three-Dimensional Substrate to Test the Differentiation Potential of Embryonic Stem Cell Derived Renal Progenitors.
abstract: The development of strategies for tissue regeneration and bio-artificial organ development is based on our understanding of embryogenesis. Differentiation protocols attempt to recapitulate the signaling modalities of gastrulation and organogenesis, coupled with cell selection regimens to isolate the cells of choice. This strategy is impeded by the lack of optimal in vitro culture systems since traditional culture systems do not allow for the three-dimensional interaction between cells and the extracellular matrix. While artificial three-dimensional scaffolds are available, using the natural extracellular matrix scaffold is advantageous because it has a distinct architecture that is difficult to replicate. The adult extracellular matrix is predicted to mediate signaling related to tissue repair not embryogenesis but existing similarities between the two argues that the extracellular matrix will influence the differentiation of stem and progenitor cells. Previous studies using undifferentiated embryonic stem cells grown directly on acellular kidney ECM demonstrated that the acellular kidney supported cell growth but limited differentiation occurred. Using mouse kidney extracellular matrix and mouse embryonic stem cells we report that the extracellular matrix can support the development of kidney structures if the stem cells are first differentiated to kidney progenitor cells before being applied to the acellular organ.
authors.affiliation: Women's and Infant's Health, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, 60 Murray St, Box 40, Toronto, ON, M5T 3L9, Canada.|Department of Physiology, University of Toronto, Toronto, Canada.
authors.forename: Manpreet
authors.initials: M
authors.lastname: Sambi
authors.affiliation: Women's and Infant's Health, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, 60 Murray St, Box 40, Toronto, ON, M5T 3L9, Canada.|Department of Physiology, University of Toronto, Toronto, Canada.
authors.forename: Theresa
authors.initials: T
authors.lastname: Chow
authors.affiliation: Women's and Infant's Health, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, 60 Murray St, Box 40, Toronto, ON, M5T 3L9, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Whiteley
authors.affiliation: Women's and Infant's Health, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, 60 Murray St, Box 40, Toronto, ON, M5T 3L9, Canada.
authors.forename: Mira
authors.initials: M
authors.lastname: Li
authors.affiliation: Women's and Infant's Health, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, 60 Murray St, Box 40, Toronto, ON, M5T 3L9, Canada.
authors.forename: Shawn
authors.initials: S
authors.lastname: Chua
authors.affiliation: Women's and Infant's Health, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, 60 Murray St, Box 40, Toronto, ON, M5T 3L9, Canada.|Department of Physiology, University of Toronto, Toronto, Canada.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Raileanu
authors.affiliation: Women's and Infant's Health, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, 60 Murray St, Box 40, Toronto, ON, M5T 3L9, Canada. Irogers@lunenfeld.ca.|Department of Physiology, University of Toronto, Toronto, Canada. Irogers@lunenfeld.ca.|Department of Obstetrics and Gynecology, Mount Sinai Hospital and the University of Toronto, Toronto, Canada. Irogers@lunenfeld.ca.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Rogers
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005109:Extracellular Matrix / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry* / Q000166:cytology*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism
keywords: Acellular; Decellularized kidney; Extracellular matrix; Kidney; Organ culture; Pluripotent cells; Six2 + ; Stem cells