title: Elementary Protein Analysis inSchizosaccharomyces pombe.
abstract: Biochemical monitoring and interrogation of protein function is a critical component of most fission yeast studies. In particular, its small proteome size, high conservation of core molecular cell biology, and genetic malleability makeSchizosaccharomyces pombean excellent model organism in which to use mass spectrometry to conduct proteome-wide approaches. Here we discuss issues encountered during the analysis of fission yeast protein preparations.
authors.affiliation: CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.uk.
authors.forename: Agnes
authors.initials: A
authors.lastname: Grallert
authors.affiliation: CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.uk.
authors.forename: Iain M
authors.initials: IM
authors.lastname: Hagan
mesh_terms: D005656:Fungal Proteins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D012568:Schizosaccharomyces / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D020543:Proteome