title: The genomic and biological characterization of Citrullus lanatus cryptic virus infecting watermelon in China.
abstract: A dsRNA virus was detected in the watermelon (Citrullus lanatus) samples collected from Kaifeng, Henan province, China through the use of next generation sequencing of small RNAs. The complete genome of this virus is comprised of dsRNA-1 (1603nt) and dsRNA-2 (1466nt), both of which are single open reading frames and potentially encode a 54.2kDa RNA-dependent RNA polymerase (RdRp) and a 45.9kDa coat protein (CP), respectively. The RdRp and CP share the highest amino acid identities 85.3% and 75.4% with a previously reported Israeli strain Citrullus lanatus cryptic virus (CiLCV), respectively. Genome comparisons indicate that this virus is the same species with CiLCV, whereas the reported sequences of the Israeli strain of CiLCV are partial, and our newly identified sequences can represent the complete genome of CiLCV. Futhermore, phylogenetic tree analyses based on the RdRp sequences suggest that CiLCV is one member in the genus Deltapartitivirus, family Partitiviridae. In addition, field investigation and seed-borne bioassays show that CiLCV commonly occurs in many varieties and is transmitted though seeds at a very high rate.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xin
authors.affiliation: National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400715, China.
authors.forename: Mengji
authors.initials: M
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Yingdang
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Chuantao
authors.initials: C
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: xfwang@ippcaas.cn.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002681:China; D029773:Citrullus / Q000821:virology*; D015870:Gene Expression; D059646:Genome Size; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000821:virology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase
keywords: Citrullus lanatus cryptic virus (CiLCV); Full genome; Next generation sequencing (NGS); Seed-borne; Watermelon (Citrullus lanatus)