title: Methods of Isolation and Characterization of Oligogalacturonide Elicitors.
abstract: Oligogalacturonides (OGs) are pectic fragments derived from the partial degradation of homogalacturonan in the plant cell wall and able to elicit plant defence responses. Recent methodological advances in the isolation of OGs from plant tissues and their characterization have confirmed their role as bona fide plant Damage-Associated Molecular Patterns. Here, we describe the methods for the isolation of OGs from Arabidopsis leaf tissues and for the characterization of OG structure and biological activity.
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors.forename: Manuel
authors.initials: M
authors.lastname: Benedetti
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Mattei
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Pontiggia
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Salvi
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185, Rome, Italy.
authors.forename: Daniel Valentin
authors.initials: DV
authors.lastname: Savatin
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185, Rome, Italy. simone.ferrari@uniroma1.it.
authors.forename: Simone
authors.initials: S
authors.lastname: Ferrari
mesh_terms: D017360:Arabidopsis / Q000276:immunology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D010368:Pectins / Q000032:analysis / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D057865:Plant Immunity; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D029681:Arabidopsis Proteins; D010455:Peptides; D010368:Pectins; C003181:polygalacturonic acid
keywords: Damage-associated molecular patterns; Elicitors; Innate immunity; Oligogalacturonides; Pectin