title: Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID.
abstract: Mesenchymal stem cells (MSCs) are blossoming as a credible source for regenerative medical applications. The use of fetal MSCs is gaining momentum for therapeutic use. The ease of isolation, enhanced characteristics, and immunomodulation properties renders the utilization of fetal MSCs for numerous clinical applications. In this article, we will demonstrate a step-by-step protocol for isolation of Wharton's jelly MSCs (WJMSCs) from the human umbilical cord matrix, preparation of human platelet lysate, fabricating amniotic membrane scaffold and mice model to study skin regeneration using a combination of MSCs and decellularized amniotic membrane scaffold.
authors.affiliation: Centre for Stem Cell Research, A Unit of inStem Bengaluru, Christian Medical College, Bagayam, Vellore, 632002, Tamil Nadu, India.
authors.forename: Vikram
authors.initials: V
authors.lastname: Sabapathy
authors.affiliation: Centre for Stem Cell Research, A Unit of inStem Bengaluru, Christian Medical College, Bagayam, Vellore, 632002, Tamil Nadu, India.
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Sundaram
authors.affiliation: Centre for Stem Cell Research, A Unit of inStem Bengaluru, Christian Medical College, Bagayam, Vellore, 632002, Tamil Nadu, India. skumar@cmcvellore.ac.in.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers; D002453:Cell Cycle; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D058948:Cell Tracking; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D016513:Mice, SCID; D010641:Phenotype; D012867:Skin / Q000293:injuries*; D059631:Wharton Jelly / Q000166:cytology*; D014945:Wound Healing
chemical_list: D015415:Biomarkers
keywords: Amniotic membrane; Indocyanine green; Mesenchymal stem cells; Platelet lysate; Scaffold; Skin injury