title: Clinical features and microbiological in bacterial keratitis in a tertiary referral hospital.
abstract: OBJECTIVE
  To describe the clinical features, bacterial agents, and antibiotic sensitivity of bacterial keratitis in the Ophthalmology Department at the University Hospital Fundación Jiménez Díaz (HUFJD) in Madrid.
  
  
  METHODS
  A retrospective observational descriptive study using clinical records and reports of corneal scrapings in patients with bacterial keratitis at the HUFJD conducted between 2009 and 2014.
  
  
  RESULTS
  In a sample of 160 patients, gram-positive bacteria were the most prevalent with 64.3% (n=103). Coagulase negative staphylococcus (20.6%), Staphylococcus aureus (19.4%), and Pseudomonas aeruginosa (12.5%) were the most frequent bacteria. The most common risk factor was the use of contact lenses, followed by disease of the ocular surface, and previous ocular surgeries. The antibiotics to which the bacteria were most commonly susceptible were gentamicin (n=114), cotrimoxazole (n=107), vancomycin (n=106), and ciprofloxacin (n=97). The antibiotics to which the bacteria were most commonly resistant were ampicillin (n=59) and erythromycin (n=45).
  
  
  CONCLUSIONS
  In the initial management of bacterial keratitis, the sensitivity and resistance of bacteria to antibiotics should be taken into account. Based on our findings, the use of aminoglycosides, vancomycin and fluoroquinolones is recommended, and, although widely used today, the discontinuation of erythromycin.
authors.affiliation: Departamento de Oftalmología, Hospital Universitario Fundación Jiménez Díaz, Madrid, España. Electronic address: drivertad@hotmail.com.
authors.forename: J M
authors.initials: JM
authors.lastname: Ruiz Caro
authors.affiliation: Departamento de Oftalmología, Hospital Universitario Fundación Jiménez Díaz, Madrid, España.
authors.forename: L
authors.initials: L
authors.lastname: Cabrejas
authors.affiliation: Instituto de Investigación Ramón Castroviejo, Madrid, España.
authors.forename: M R
authors.initials: MR
authors.lastname: de Hoz
authors.affiliation: Departamento de Oftalmología, Hospital Universitario Fundación Jiménez Díaz, Madrid, España.
authors.forename: D
authors.initials: D
authors.lastname: Mingo
authors.affiliation: Departamento de Oftalmología, Hospital Universitario Fundación Jiménez Díaz, Madrid, España.
authors.forename: S P
authors.initials: SP
authors.lastname: Duran
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects; D015818:Eye Infections, Bacterial / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology; D005260:Female; D006801:Humans; D007634:Keratitis / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D012189:Retrospective Studies; D062606:Tertiary Care Centers
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic resistance; Bacterial keratitis; Características clínicas; Clinical features; Queratitis bacteriana; Resistencia antibiótica