title: Systemic Identification ofHevea brasiliensisEST-SSR Markers and Primer Screening.
abstract: This research aimed to systematically identify and preliminarily validate theHevea brasiliensisexpressed sequence tag (EST) information using Simple Sequence Repeat (SSR) and provide evidence for further development of SSR molecular marker. The definition of general SSR features ofHeveaEST splicing sequences and development of SSR primers founded the basis of diversity analysis and variety identification forHeveatree resource. 1134 SSR loci were identified in the EST splicing sequence and distributed in 840 Unigene. The occurrence rate of SSR loci was 23.9%, and the average distribution distance of EST-SSR was 2.59 kb. The major repeat type was mononucleotide repeat motif, which accounted for 38.89%, while the corresponding value was 36.95% for dinucleotide repeat motif and 18.17% for trinucleotide repeat motif; the proportion of other motifs was only 5.99%. The superior repeat motifs for mononucleotide, dinucleotide, and trinucleotide were A/T, AG/CT, and AAG/CTT, respectively. 739 pair of primers were designed for 1134 SSR loci. PCR amplification was performed onHeveaReyan5-11, Reyan87-6-47, and PR107, and 180 pairs of primers were selected which were able to amplify polymorphism bands.
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China; Institute of Cereal Research, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Benjun
authors.initials: B
authors.lastname: Hou
authors.affiliation: Hainan Tropical Ocean University, Sanya 572202, China; Key Laboratory of Tropical Crop Molecular Breeding of Sanya, Sanya 572202, China.
authors.forename: Suping
authors.identifier: 0000-0002-4482-7486
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, China; Key Laboratory of Tropical Crop Molecular Breeding of Sanya, Sanya 572202, China.
authors.forename: Yaoting
authors.identifier: 0000-0001-5181-3982
authors.initials: Y
authors.lastname: Wu