title: Reconstruction of non-uniformly sampled five-dimensional NMR spectra by signal separation algorithm.
abstract: A method for five-dimensional spectral reconstruction of non-uniformly sampled NMR data sets is proposed. It is derived from the previously published signal separation algorithm, with major alterations to avoid unfeasible processing of an entire five-dimensional spectrum. The proposed method allows credible reconstruction of spectra from as little as a few hundred data points and enables sensitive resonance detection in experiments with a high dynamic range of peak intensities. The efficiency of the method is demonstrated on two high-resolution spectra for rapid sequential assignment of intrinsically disordered proteins, namely 5D HN(CA)CONH and 5D (HACA)CON(CO)CONH.
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kosiński
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Stanek
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.|Faculty of Physics, Division of Biophysics, Institute of Experimental Physics, University of Warsaw, Pasteura 5, 02-093, Warsaw, Poland.
authors.forename: Michał J
authors.initials: MJ
authors.lastname: Górka
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Żerko
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland. kozmin@chem.uw.edu.pl.
authors.forename: Wiktor
authors.initials: W
authors.lastname: Koźmiński
mesh_terms: D000465:Algorithms; D002247:Carbon Isotopes; D006801:Humans; D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D059629:Signal-To-Noise Ratio; D051844:alpha-Synuclein / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D064267:Intrinsically Disordered Proteins; D009587:Nitrogen Isotopes; D051844:alpha-Synuclein
keywords: 5D; High-dimensional NMR; Intrinsically disordered proteins; Non-uniform sampling; Resonance assignment; Signal separation algorithm; Spectral reconstruction