title: Application of a Stir Bar Sorptive Extraction sample preparation method with HPLC for soil fungal biomass determination in soils from a detrital manipulation study.
abstract: Ergosterol is a sterol found ubiquitously in cell membranes of filamentous fungi. Although concentrations in different fungal species span the range of 2.6 to 42μg/mL of dry mass, many studies have shown a strong correlation between soil ergosterol content and fungal biomass. The analysis of ergosterol in soil therefore could be an effective tool for monitoring changes in fungal biomass under different environmental conditions. Stir Bar Sorptive Extraction (SBSE) is a new sample preparation method to extract and concentrate organic analytes from liquid samples. SBSE was here demonstrated to be a simple, fast, and cost effective method for the quantitative analysis of ergosterol from field-collected soils. Using this method we observed that soil ergosterol as a measure of fungal biomass proved to be a sensitive indicator of soil microbial dynamics that were altered by changes in plant detrital inputs to soils in a long-term field experiment.
authors.affiliation: Institute of Agricultural Chemistry and Soil Science, University of Debrecen, Böszörményi út 138, Debrecen, Hungary. Electronic address: beniaron@agr.unideb.hu.
authors.forename: Áron
authors.initials: Á
authors.lastname: Beni
authors.affiliation: Department of Crop and Soil Science, Oregon State University, 3017 Agricultural and Life Sciences Building, Corvallis, OR 97331, USA.
authors.forename: Kate
authors.initials: K
authors.lastname: Lajtha
authors.affiliation: Institute of Environmental Sciences, University of Nyiregyhaza, Sóstói út 31/B, Nyiregyhaza, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Kozma
authors.affiliation: Institute of Environmental Sciences, University of Nyiregyhaza, Sóstói út 31/B, Nyiregyhaza, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Fekete
mesh_terms: D018533:Biomass; D005591:Chemical Fractionation / Q000295:instrumentation* / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D004875:Ergosterol / Q000032:analysis* / Q000737:chemistry; D005658:Fungi / Q000737:chemistry* / Q000302:isolation & purification; D006814:Hungary; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D012987:Soil; D012965:Sodium Chloride; D006851:Hydrochloric Acid; D004875:Ergosterol
keywords: DIRT; Ergosterol; Fungal biomass; HPLC; SBSE