title: Catechol Groups Enable Reactive Oxygen Species Scavenging-Mediated Suppression of PKD-NFkappaB-IL-8 Signaling Pathway by Chlorogenic and Caffeic Acids in Human Intestinal Cells.
abstract: Chlorogenic acid (CHA) and caffeic acid (CA) are phenolic compounds found in coffee, which inhibit oxidative stress-induced interleukin (IL)-8 production in intestinal epithelial cells, thereby suppressing serious cellular injury and inflammatory intestinal diseases. Therefore, we investigated the anti-inflammatory mechanism of CHA and CA, both of which inhibited hydrogen peroxide (H₂O₂)-inducedIL-8transcriptional activity. They also significantly suppressed nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) transcriptional activity, nuclear translocation of the p65 subunit, and phosphorylation of IκB kinase (IKK). Additionally, upstream of IKK, protein kinase D (PKD) was also suppressed. Finally, we found that they scavenged H₂O₂-induced reactive oxygen species (ROS) and the functional moiety responsible for the anti-inflammatory effects of CHA and CA was the catechol group. Therefore, we conclude that the presence of catechol groups in CHA and CA allows scavenging of intracellular ROS, thereby inhibiting H₂O₂-induced IL-8 production via suppression of PKD-NF-κB signaling in human intestinal epithelial cells.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. hsshin@kfri.re.kr.|Division of Nutrition and Metabolism Research, Korea Food Research Institute, Seongnam-si 13539, Korea. hsshin@kfri.re.kr.|Department of Food Biotechnology, University of Science and Technology (UST), Daejeon 34113, Korea. hsshin@kfri.re.kr.
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. satsu@maebashi-it.ac.jp.|Department of Biotechnology, Faculty of Engineering, Maebashi Institute of Technology, Gunma 371-0816, Japan. satsu@maebashi-it.ac.jp.
authors.forename: Hideo
authors.initials: H
authors.lastname: Satsu
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. japanpaper04@gmail.com.|Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 08826, Korea. japanpaper04@gmail.com.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Bae
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. atotuka@mail.ecc.u-tokyo.ac.jp.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Totsuka
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. ms205346@nodai.ac.jp.|Department of Nutritional Science, Tokyo University of Agriculture, Tokyo 156-8502, Japan. ms205346@nodai.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D018938:Caco-2 Cells; D002109:Caffeic Acids / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D002726:Chlorogenic Acid / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002109:Caffeic Acids; D002396:Catechols; D016209:Interleukin-8; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; D006861:Hydrogen Peroxide; C089212:protein kinase D; D011493:Protein Kinase C; C034221:catechol; C040048:caffeic acid
keywords: caffeic acid; catechol group; chlorogenic acid; interleukine-8; nuclear factor κB; protein kinase D; reactive oxygen species