title: Alterations in Soluble Class III Peroxidases of Maize Shoots by Flooding Stress.
abstract: Due to changing climate, flooding (waterlogged soils and submergence) becomes a major problem in agriculture and crop production. In the present study, the effect of waterlogging was investigated on peroxidases of maize (Zea maysL.) leaves. The plants showed typical adaptations to flooding stress,i.e., alterations in chlorophylla/bratios and increased basal shoot diameter. Seven peroxidase bands could be detected by first dimension modified SDS-PAGE and 10 bands by first dimension high resolution Clear Native Electrophoresis that altered in dependence on plant development and time of waterlogging. Native isoelectric focusing revealed three acidic to neutral and four alkaline guaiacol peroxidases that could be further separated by high resolution Clear Native Electrophorese in the second dimension. One neutral peroxidase (pI 7.0) appeared to be down-regulated within four hours after flooding, whereas alkaline peroxidases (pI 9.2, 8.0 and 7.8) were up-regulated after 28 or 52 h. Second dimensions revealed molecular masses of 133 kDa and 85 kDa for peroxidases at pI 8.0 and 7.8, respectively. Size exclusion chromatography revealed native molecular masses of 30-58 kDa for peroxidases identified as class III peroxidases and ascorbate peroxidases by mass spectrometry. Possible functions of these peroxidases in flooding stress will be discussed.
authors.affiliation: University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Oxidative Stress and Plant Proteomics Group, Ohnhorststraße 18, D-22609 Hamburg, Germany. c_m_2406@yahoo.de.
authors.forename: Claudia-Nicole
authors.initials: CN
authors.lastname: Meisrimler
authors.affiliation: University Hospital Eppendorf, Institute of Clinical Chemistry, Campus Science, Martinistraße 52, D-20246 Hamburg, Germany. buck@uke.de.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Buck
authors.affiliation: University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Oxidative Stress and Plant Proteomics Group, Ohnhorststraße 18, D-22609 Hamburg, Germany. s.luthje@botanik.uni-hamburg.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Lüthje
keywords: Zea mays L.; flooding; guaiacol peroxidases; native PAGE; shoot; soluble proteins; water logging