title: β-Catenin overexpression causes an increase in inflammatory cytokines and NF-κB activation in cardiomyocytes.
abstract: β-Catenin has been implicated in various developmental and physiological processes. Defective Wnt signaling can result in different cardiac and vascular abnormalities and is activated under pathological conditions such as inflammation and obesity. In this study, roles of β-catenin in inflammation in cardiomyocytes were investigated. 10 samples from hearts of patients with acute infarction and 10 from normal ones were collected in order to access roles of β-catenin in cardiomyocytes. H9c2 cardiomyoblasts and primary neonatal rat cardiomyocytes were transfected with porcine cytomegalovirus (pCMV)-β-catenin plasmid in order to overexpress β-catenin. Protein level of β-catenin protein was increased in human acute infarction tissues compared to ones from normal patients. The transcription factor had increased nuclear localization in cardiomyocytes of the Wistar rats with cardiac hypertension. Furthermore, expression of fibrosis protein markers increased. Protein expression of β-catenin was increased in human acute infarction inflammatory heart tissues and in hearts of inflammatory obesity rats. After pCMV-β-catenin plasmid was transfected in a dose-dependent manner, inflammation protein markers, TNF-α and IL-8, were upregulated in hypertensive neonatal rat cardiomyocytes and H9c2 cardiomyoblasts. In addition, overexpression of β-catenin induced activation and nuclear localization of NF-κB. Therefore, β-catenin is a potential molecular target for treatment of inflammation and fibrosis in cardiomyocytes.
authors.affiliation: Department of Occupational Therapy, Asia University, Taichung 41354, Taiwan.
authors.forename: J C
authors.initials: JC
authors.lastname: Lin
authors.affiliation: College of Chinese Medicine, School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung City, Taiwan.
authors.forename: R-L
authors.initials: RL
authors.lastname: Chang
authors.affiliation: Department of Obstetrics and Gynecology, Taichung Veteran's General Hospital, Taichung 40705, Taiwan.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Chen
authors.affiliation: Institute of Oral Sciences, College of Oral Medicine, Chung Shan Medical University, Taichung 40201, Taiwan.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan.
authors.forename: R
authors.initials: R
authors.lastname: Baskaran
authors.affiliation: Department of Hospital and Health Care Administration, Chia Nan University of Pharmacy & Science, Tainan County 71710 , Taiwan.
authors.forename: L-C
authors.initials: LC
authors.lastname: Chung
authors.affiliation: Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031 ,Taiwan.
authors.forename: R-J
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: Department of Nursing, MeiHo University, Pingtung County 91202, Taiwan.
authors.forename: C H
authors.initials: CH
authors.lastname: Day
authors.affiliation: Department of Biotechnology, Bharathiar University, Coimbatore-641 046, India.
authors.forename: V
authors.initials: V
authors.lastname: Vijaya Padma
authors.affiliation: Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Huang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D016209:Interleukin-8; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D051176:beta Catenin