title: Derivation of Mesenchymal Stem Cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells.
abstract: Mesenchymal stem cells (MSCs) provide an opportunity to bring the field of regenerative medicine to realization. A lot of clinical trials are presently trying to establish their applicability in real-world scenarios. Some of the biggest challenges encountered in bringing MSCs from bench to bedside are the number of MSCs required, their procurement from various sources, and the batch-to-batch variability. This often leads to inconclusive results within and between different studies. Therefore, we have hereby proposed a simple protocol to source mesenchymal stem cells through differentiation of embryonic stem cells.
authors.affiliation: Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, R 3028-2, 351 Tache Avenue, Winnipeg, MB, Canada, R2H2A6.
authors.forename: Glen Lester
authors.initials: GL
authors.lastname: Sequiera
authors.affiliation: Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, R 3028-2, 351 Tache Avenue, Winnipeg, MB, Canada, R2H2A6.
authors.forename: Niketa
authors.initials: N
authors.lastname: Sareen
authors.affiliation: Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, R 3028-2, 351 Tache Avenue, Winnipeg, MB, Canada, R2H2A6.
authors.forename: Ejlal Abu
authors.initials: EA
authors.lastname: El-Rub
authors.affiliation: Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, R 3028-2, 351 Tache Avenue, Winnipeg, MB, Canada, R2H2A6. sdhingra@sbrc.ca.
authors.forename: Sanjiv
authors.initials: S
authors.lastname: Dhingra
mesh_terms: D053687:Adult Stem Cells; D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005347:Fibroblasts; D016130:Immunophenotyping / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D044968:Regenerative Medicine
chemical_list: D015415:Biomarkers
keywords: Batch variation; Differentiation; Embryonic stem cells; Mesenchymal stem cells; Non-Variable MSC source; Regenerative medicine