title: Comparison between resection, bipolar coagulation and Plasmajet®: A preliminary animal study.
abstract: OBJECTIVE
  To compare the most used types of surgical techniques, for peritoneal lesions management, to Plasmajet® (PJ), in term of healing and post-operative adhesion.
  
  
  METHODS
  Prospective, experimental animal study. Female pigs (Landrace/Large White-Pietran) weighing 20-25kgs were used for the experiments. Eleven areas of 2cm2were treated on each lateral side of the peritoneal wall. Two areas of control, 2 of surgical resection with scissors, 2 of bipolar coagulation, 2 of Plasmajet® 10 low (PJ10L, adjustment of the Plasmajet®) used in contact with the peritoneum, 2 of PJ10L used at 3-5mm from the peritoneum, 2 of PJ10L used at 10mm, 2 of PJ used at 10 High (PJ10H) close to the peritoneum, 2 of PJ10H used at 3-5mm, 2 of PJ10H used at 10mm, 2 of PJ used at 40 Low (PJ40L) used at 3-5mm, 2 of PJ40L used at 10mm from the peritoneum.
  
  
  RESULTS
  For each 2 areas, one was removed immediately for histological analysis. All animals were reoperated 14days later to evaluate macroscopic healing, adhesion score, histological inflammation and mesothelialization. Immediate histological analysis shows that in every treated area the peritoneum was completely vaporized, coagulated or removed. After resection, the healing was macroscopically perfect and there was no adhesion, as in the control area. After bipolar coagulation in half of cases there was adhesion. There was no adhesion after treatment by Plasmajet® 10 low used at 10mm from the peritoneum.
  
  
  CONCLUSIONS
  Surgical resection leads to perfect healing, and no adhesion formation. The use of Plasmajet® 10 low used at 10mm from the peritoneum could be an alternative to resection, because it allows complete superficial destruction, with a low rate of adhesion. Further study is required to explore and assess fully the potential of this device.
authors.affiliation: Department of Gynecologic Surgery, CHU Estaing, 1 place Lucie Aubrac, 63058 Clermont-Ferrand Cedex 1, France; CICE (Centre International de Chirurgie Endoscopique), Faculty of Medicine, Clermont-Ferrand, France(1). Electronic address: nicolas.bourdel@gmail.com.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bourdel
authors.affiliation: Department of Gynecologic Surgery, CHU Estaing, 1 place Lucie Aubrac, 63058 Clermont-Ferrand Cedex 1, France; CICE (Centre International de Chirurgie Endoscopique), Faculty of Medicine, Clermont-Ferrand, France(1).
authors.forename: Pauline
authors.initials: P
authors.lastname: Chauvet
authors.affiliation: Department of Gynecology and Obstetrics, Rouen University Hospital, France; Research Group 4308, Spermatogenesis and Gamete Quality, IHU Rouen Normandy, IFRMP23, Reproductive Biology Laboratory, Rouen University Hospital, 1 Rue de Germont, 76000 Rouen, France.
authors.forename: Horace
authors.initials: H
authors.lastname: Roman
authors.affiliation: University Hospital Clermont-Ferrand, Biostatistics unit (DRCI), 58, Rue Montalembert, 63003 Clermont-Ferrand cedex, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Pereira
authors.affiliation: Department of Pathology, Rouen University Hospital, 1 Rue de Germont, 76000 Rouen, France.
authors.forename: Oana
authors.initials: O
authors.lastname: Somcutian
authors.affiliation: Department of Pathology, Clermont-Ferrand University Hospital, 1 place Lucie Aubrac, 63058 Clermont-Ferrand Cedex 1, France.
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Dechelotte
authors.affiliation: Department of Gynecologic Surgery, CHU Estaing, 1 place Lucie Aubrac, 63058 Clermont-Ferrand Cedex 1, France; CICE (Centre International de Chirurgie Endoscopique), Faculty of Medicine, Clermont-Ferrand, France(1).
authors.forename: Michel
authors.initials: M
authors.lastname: Canis
mesh_terms: D000818:Animals; D004564:Electrocoagulation / Q000009:adverse effects / Q000379:methods*; D004715:Endometriosis / Q000473:pathology / Q000601:surgery*; D005260:Female; D010532:Peritoneal Diseases / Q000473:pathology / Q000601:surgery*; D010537:Peritoneum / Q000473:pathology / Q000601:surgery*; D011183:Postoperative Complications / Q000209:etiology; D013552:Swine; D000267:Tissue Adhesions / Q000209:etiology
keywords: Adhesion; Bipolar coagulation; Healing; Plasmajet®; Resection