title: Characterization of microsatellite markers in two exploited African trees,Entandrophragma candolleiandE. utile(Meliaceae).
abstract: OBJECTIVE
  Multiplexes of nuclear microsatellite primers were developed to investigate population genetic structure and diversity in two exploited African rainforest trees:Entandrophragma candolleiandE. utile(Meliaceae).
  
  
  RESULTS
  Microsatellite isolation was performed simultaneously on two nonenriched genomic libraries after next-generation sequencing. We developed 16 and 22 polymorphic markers forE. candolleiandE. utilein three and four multiplexes, respectively. The number of alleles ranged from two to 17 forE. candolleiand from three to 19 forE. utile. Mean expected and observed heterozygosity ranged between 0.75 ± 0.13 and 0.55 ± 0.23 forE. candolleiand between 0.73 ± 0.10 and 0.49 ± 0.2 forE. utile.
  
  
  CONCLUSIONS
  These sets of nuclear microsatellite markers constitute useful tools for exploring gene flow patterns in these twoEntandrophragmaspecies.
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
authors.forename: Franck S
authors.initials: FS
authors.lastname: Monthe
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium; Institut de Recherche pour le Développement, UMR-DIADE, BP 64501, 34394 Montpellier, France; Bioversity International, Forest Genetic Resources Programme, Sub-Regional Office for Central Africa, P.O. Box 2008 Messa, Yaoundé, Cameroon.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Duminil
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium; TERRA Research Centre, Central African Forests, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium.
authors.forename: Félicien
authors.initials: F
authors.lastname: Tosso
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Migliore
authors.affiliation: Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
authors.forename: Olivier J
authors.initials: OJ
authors.lastname: Hardy
keywords: Entandrophragma; Meliaceae; gene flow; microsatellites; next-generation sequencing