title: Extraction and isolation of potential anti-stroke compounds from flowers of Pueraria lobata guided by in vitro PC12 cell model.
abstract: A simple and efficient method based on ultrafiltration liquid chromatography-mass spectrometry (UFLC-MS) was applied to rapidly screen and identify ligands for lactate dehydrogenase (LDH) from the flowers of Pueraria lobata, and the compounds were assessed for anti-stroke activity using a PC12 cell model. Seven major isoflavones, kakkalide, 3'-hydroxy puerarin, puerarin, puerarin xyloside, tectoridin, tectorigenin, and ononin, were identified as potent LDH inhibitors. A continuous online method, which consisted of microwave-assisted extraction and countercurrent chromatography (MAE-CCC), was newly developed for scaled-up production of these compounds with high purity and efficiency. This novel approach, using UFLC-MS coupled with MAE-CCC and a PC12 cell model, provided a powerful tool for screening, extraction, and separation of LDH inhibitors from complex samples, and a useful platform for the large-scale production of functional food and nutraceutical ingredients.
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Chang-ji Road, Changchun 130032, China.
authors.forename: Sainan
authors.initials: S
authors.lastname: Li
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Chang-ji Road, Changchun 130032, China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Li
authors.affiliation: Clinical Department of Rehabilitation, College of Acupuncture and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. Electronic address: 14042234@qq.com.
authors.forename: Chengyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Chang-ji Road, Changchun 130032, China. Electronic address: anal_chem@sina.cn.
authors.forename: Chunming
authors.initials: C
authors.lastname: Liu
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Chang-ji Road, Changchun 130032, China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004867:Equipment Design; D035264:Flowers / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008024:Ligands; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008872:Microwaves; D016716:PC12 Cells; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods
chemical_list: D004791:Enzyme Inhibitors; D007529:Isoflavones; D008024:Ligands; D010936:Plant Extracts; D007770:L-Lactate Dehydrogenase
keywords: Countercurrent chromatography; Flowers of Pueraria lobata; Lactate dehydrogenase inhibitors; Microwave-assisted extraction; Stroke