title: High Performance Reduction of H2O2with an Electron Transport Decaheme Cytochrome on a Porous ITO Electrode.
abstract: The decaheme cytochrome MtrC from Shewanella oneidensis MR-1 immobilized on an ITO electrode displays unprecedented H2O2reduction activity. Although MtrC showed lower peroxidase activity in solution compared to horseradish peroxidase, the ten heme cofactors enable excellent electronic communication and a superior activity on the electrode surface. A hierarchical ITO electrode enabled optimal immobilization of MtrC and a high current density of 1 mA cm-2at 0.4 V vs SHE could be obtained at pH 6.5 (Eonset= 0.72 V). UV-visible and Resonance Raman spectroelectrochemical studies suggest the formation of a high valent iron-oxo species as the catalytic intermediate. Our findings demonstrate the potential of multiheme cytochromes to catalyze technologically relevant reactions and establish MtrC as a new benchmark in biotechnological H2O2reduction with scope for applications in fuel cells and biosensors.
authors.affiliation: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Reuillard
authors.affiliation: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
authors.forename: Khoa H
authors.initials: KH
authors.lastname: Ly
authors.affiliation: Institut für Chemie, Technische Universität Berlin , Sekr. PC14, Straße des 17. Juni 135, D-10623 Berlin, Germany.
authors.forename: Peter
authors.identifier: 0000-0003-1030-5900
authors.initials: P
authors.lastname: Hildebrandt
authors.affiliation: School of Biomedical Sciences and the Astbury Centre, University of Leeds , Leeds LS2 9JT, United Kingdom.
authors.forename: Lars J C
authors.identifier: 0000-0001-7810-3964
authors.initials: LJ
authors.lastname: Jeuken
authors.affiliation: School of Chemistry and School of Biological Sciences, University of East Anglia , Norwich Research Park, Norwich NR4 7TJ, United Kingdom.
authors.forename: Julea N
authors.identifier: 0000-0002-9624-5226
authors.initials: JN
authors.lastname: Butt
authors.affiliation: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
authors.forename: Erwin
authors.identifier: 0000-0002-7781-1616
authors.initials: E
authors.lastname: Reisner