title: Maternal exposure to perfluorooctanoic acid inhibits luteal function via oxidative stress and apoptosis in pregnant mice.
abstract: Perfluorooctanoic acid (PFOA) is a synthetic perfluorinated compound, which has been reported to exert adverse effect on the pregnancy. However, whether it is associated with alteration of luteal function remains unknown. Mice were administered PFOA by gavage from gestational days (GD) 1-7 or 13. PFOA treatment did not significantly affect numbers of embryo implantation. Nevertheless, on GD 13, 10mg/kg PFOA treatment significantly increased numbers of resorbed embryo. Furthermore, PFOA exposure markedly reduced serum progesterone levels but did not affect estradiol levels. Treatment also showed concomitant decreases in transcript levels for key steroidogenic enzymes, and reduced numbers and sizes of corpora lutea. In addition, PFOA administration inhibited activities of superoxide dismutase and catalase, and increased generation of hydrogen peroxide and malondialdehyde, and down-regulated level of Bcl-2 and up-regulated p53 and BAX proteins. In conclusion, PFOA exposure significantly inhibits luteal function via oxidative stress and apoptosis in pregnant mice.
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: yilua_chen@163.com.
authors.forename: Yilu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: noradrenalin@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: xjj0605@163.com.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 13393733537@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 544182657@qq.com.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 1120434253@qq.com.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Xie
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 1454503826@qq.com.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: thinker_20080502@126.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: zhangdalei@ncu.edu.cn.
authors.forename: Dalei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: physiology9909@163.com.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: yangbei@ncu.edu.cn.
authors.forename: Bei
authors.initials: B
authors.lastname: Yang
authors.affiliation: Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: kuanghaibin@ncu.edu.cn.
authors.forename: Haibin
authors.initials: H
authors.lastname: Kuang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002210:Caprylates / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D010064:Embryo Implantation / Q000187:drug effects; D004958:Estradiol / Q000097:blood; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009097:Multienzyme Complexes / Q000235:genetics; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010750:Phosphoproteins / Q000235:genetics; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011375:Progesterone Reductase / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013251:Steroid Isomerases / Q000235:genetics; D013482:Superoxide Dismutase / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: C021425:3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase; D002210:Caprylates; D005466:Fluorocarbons; D009097:Multienzyme Complexes; D010750:Phosphoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D000098682:Steroidogenic Acute Regulatory Protein; D011374:Progesterone; D004958:Estradiol; D008315:Malondialdehyde; C023036:perfluorooctanoic acid; D006861:Hydrogen Peroxide; D011375:Progesterone Reductase; D002374:Catalase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D013482:Superoxide Dismutase; D013251:Steroid Isomerases
keywords: Apoptosis; Luteal function; Oxidative stress; Perfluorooctanoic acid; Reproductive toxicity