title: Simple on-line pretreatment of column-switching coupled with ion chromatography for the determination of lactic acid in lobaplatin.
abstract: In this study, a simple and sensitive on-line pretreatment of column-switching technique coupled with ion chromatography method was achieved for the determination of key impurity of lactic acid in lobaplatin. The first dimension of reverse phase liquid chromatography was used to reduce organic matrices, whereas the second dimension of ion chromatography was applied for the separation and detection of lactic acid. The results exhibited good linearity (R=0.9994) ranging from 0.01mgL-1to 50mgL-1. The limit of detection was below 0.0015mgL-1. Also, a spiking study was performed with satisfactory recoveries between 96% and 106%.
authors.affiliation: College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China; Institute for the Development of Hainan International Tourism Island, Haikou 570228, China. Electronic address: zhaozhendong@hainu.edu.cn.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Food Science and Technology, Hainan University, Haikou 570228, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Xie
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods; D003503:Cyclobutanes / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D057230:Limit of Detection; D009944:Organoplatinum Compounds / Q000737:chemistry*
chemical_list: D003503:Cyclobutanes; D009944:Organoplatinum Compounds; D019344:Lactic Acid; C066228:lobaplatin
keywords: Column-switching; Determination; Impurity; Ion chromatography; Lactic acid; Lobaplatin