title: Association of HLA alleles (A, B, DRB1) and HIV-1 infection in the Han population of Hubei, China.
abstract: The HIV susceptibility and resistance alleles in the HLA genes were determined by investigating the distribution characteristics of the HLA alleles (A, B, and DRB1) in HIV-infected individuals of the Han population in Hubei, and by comparing these alleles with HIV-negative individuals from the same area. A cohort of 424 HIV-1 infected individuals were chosen as study subjects, and 836 HIV-negative healthy subjects from the same area served as the control population. HLA-A, B, and DRB1 allele typing was performed using polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) and polymerase chain reaction-sequencing based typing (PCR-SBT) techniques. Arlequin ver3.0 was used to analyze the allele and haplotype frequencies of HLA-A, B, and DRB 1, whereas Epi Info 7 and SPSS18.0 was used to analyze the differences in the HLA alleles between the HIV-1 positive and HIV-1 negative groups. A*02:03, DRB1*01:01, and DRB1*15:01 alleles and their haplotypes as well as the HLA_Bw4-Bw6 hybrid showed a protective effect on HIV-1 infection. After adjusting for confounding factors such as age and sex, multivariate logistic regression analysis revealed that B*15:02G, DRB1*01:01, and DRB1*15:01 subtypes were the resistance genes of HIV-1 infection, while B*13:01 might increase susceptibility to HIV-1 infection. The correlation between A*02:06 and B*15:01G subtypes and HIV-1 susceptibility was independent of the age and sex of the host. This study demonstrated the influence of genetic factors in humans such as HLA polymorphism on individuals to resist HIV-1 infection. Association studies of HLA polymorphism, susceptibility/resistance to HIV-1 infection, and hosts' genetic background are of significant importance for research on HIV-1 pathogenesis and vaccine design.
authors.affiliation: HLA Typing Laboratory, Wuhan Blood Center, Wuhan, 430030, China. xja33@163.com.
authors.forename: Wang-Xia
authors.initials: WX
authors.lastname: Li
authors.affiliation: Department of Neurology, Medical Treatment Center of Wuhan, Wuhan, 430024, China.
authors.forename: Jia-An
authors.initials: JA
authors.lastname: Xia
authors.affiliation: Department of Neurology, Medical Treatment Center of Wuhan, Wuhan, 430024, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhou
authors.affiliation: HLA Typing Laboratory, Wuhan Blood Center, Wuhan, 430030, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: HLA Typing Laboratory, Wuhan Blood Center, Wuhan, 430030, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.affiliation: HLA Typing Laboratory, Wuhan Blood Center, Wuhan, 430030, China.
authors.forename: Feng-Wu
authors.initials: FW
authors.lastname: Qiu
mesh_terms: D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D015658:HIV Infections / Q000208:ethnology / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D059811:HLA-DRB1 Chains
keywords: China; allele; human immunodeficiency virus; human leukocyte antigen; resistance genes; susceptibility gene