title: CD8+effector memory T cells induce acute rejection of allogeneic heart retransplants in mice possibly through activating expression of inflammatory cytokines.
abstract: To investigate the effects of CD8+memory T (Tm) cells and CD8+effector memory T (Tem) cells on the results of allogeneic heart retransplantations performed in mice.
  
  
  
  A skin transplantation model was used to generate sensitized splenic CD8+Tem cells for infusion into BALB/c mice. One week after infusion, the BALB/c mice underwent allogeneic heart transplantation in the abdominal cavity. Cyclosporin A was administered via intraperitoneal injection starting one day prior to transplantation to arrest immunological rejection of the transplanted heart. The effects of sensitized CD8+Temcells on allogeneic heart graft rejection were examined by monitoring survival of the transplanted hearts, the infiltration of effector memory CD8+T cells into myocardium, and expressions of inflammatory cytokines in blood serum.
  
  
  
  Adoptive transfer of sensitized CD8+Tem cells prior to transplantation induced an acute rejection response which decreased the survival of transplanted hearts. The rejection response was accompanied by an infiltration of CD8+Tem cells into the transplanted myocardial tissue. Additionally, infusion of sensitized CD8+Tem cells induced markedly increased expressions of IL-2 and IFN-γ, and decreased expression of TGF-β in the transplanted hearts, as well as higher levels of IFN-γ and CXCL-9 in blood serum.
  
  
  
  The infusion of sensitized CD8+Tem cells induced an acute graft rejection response and decreased the survival of grafted hearts by regulating the expressions of inflammatory cytokines including CXCL-9, IL-2, and INF-γ. Cyclosporin A had no therapeutic effect on the graft rejection response induced by sensitized CD8+Tem cells.
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China; The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China; The Department of Immunology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Du
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China; The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China; The Department of Immunology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Yang
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Gong
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China; The Department of Immunology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China; The Department of Immunology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Zhou
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China; The Department of Immunology, Guangxi Medical University, Nanning 530021, China. Electronic address: luwuliu@163.com.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lu
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China; Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Guangxi Medical University, Nanning 530021, China; The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China; The Department of Immunology, Guangxi Medical University, Nanning 530021, China. Electronic address: yongxiang_zhao@126.com.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000208:Acute Disease; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D016027:Heart Transplantation; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines
keywords: Allograft rejection; CD8(+) effector memory T cells; CD8(+) memory T cells