title: Enhancement of the antiviral activity against caprine herpesvirus type 1 of Acyclovir in association with Mizoribine.
abstract: Caprine herpesvirus 1 (CpHV-1) infection in goats is responsible for genital lesions resembling the lesions induced by herpesvirus 2 in humans (HHV-2). The immunosuppressive drug Mizoribine (MIZ) is able to increase the antiviral activity of Acyclovir (ACV) against herpesvirus infections, raising interesting perspectives on new combined therapeutic strategies. In this study the anti-CpHV-1 activity in vitro of ACV alone or in combination with MIZ was evaluated. ACV (100μg/ml) displayed an antiviral effect on CpHV-1 replication. This inhibitory effect was higher when ACV (100μg/ml) was used in association with MIZ (20μg/ml). Other combinations of ACV and MIZ in various concentrations were not as effective as ACV 100μg/ml/MIZ 20μg/ml. These findings suggest that the association of ACV and MIZ is potentially useful for treatment of genital infection by herpesviruses.
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Camero
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Buonavoglia
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Lucente
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Losurdo
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Crescenzo
authors.affiliation: Department of Biomedical Science and Human Oncology, University of Bari, Medical School, Bari, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Trerotoli
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Casalino
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.affiliation: Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy. Electronic address: maria.tempesta@uniba.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Tempesta
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D015511:Goat Diseases / Q000188:drug therapy* / Q000821:virology; D006041:Goats; D006566:Herpesviridae Infections / Q000188:drug therapy / Q000662:veterinary* / Q000821:virology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D012263:Ribonucleosides / Q000494:pharmacology*; D018140:Varicellovirus / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D007166:Immunosuppressive Agents; D012263:Ribonucleosides; C010052:mizoribine; D000212:Acyclovir
keywords: Acyclovir; Antiviral activity; Association; Caprine herpesvirus 1; In vitro; Mizoribine