title: Differential Gene Expression Analysis inPolygonum minusLeaf upon 24 h of Methyl Jasmonate Elicitation.
abstract: Polygonum minusis an herbal plant that grows in Southeast Asian countries and traditionally used as medicine. This plant produces diverse secondary metabolites such as phenolic compounds and their derivatives, which are known to have roles in plant abiotic and biotic stress responses. Methyl jasmonate (MeJA) is a plant signaling molecule that triggers transcriptional reprogramming in secondary metabolism and activation of defense responses against many biotic and abiotic stresses. However, the effect of MeJA elicitation on the genome-wide expression profile in the leaf tissue ofP. minushas not been well-studied due to the limited genetic information. Hence, we performed Illumina paired-end RNA-seq forde novoreconstruction ofP. minusleaf transcriptome to identify differentially expressed genes (DEGs) in response to MeJA elicitation. A total of 182,111 unique transcripts (UTs) were obtained byde novoassembly of 191.57 million paired-end clean reads using Trinity analysis pipeline. A total of 2374 UTs were identified to be significantly up-/down-regulated 24 h after MeJA treatment. These UTs comprising many genes related to plant secondary metabolite biosynthesis, defense and stress responses. To validate our sequencing results, we analyzed the expression of 21 selected DEGs by quantitative real-time PCR and found a good correlation between the two analyses. The single time-point analysis in this work not only provides a useful genomic resource forP. minusbut also gives insights on molecular mechanisms of stress responses inP. minus.
authors.affiliation: Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia Bangi, Malaysia.
authors.forename: Reyhaneh
authors.initials: R
authors.lastname: Rahnamaie-Tajadod
authors.affiliation: Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia Bangi, Malaysia.
authors.forename: Kok-Keong
authors.initials: KK
authors.lastname: Loke
authors.affiliation: Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia Bangi, Malaysia.
authors.forename: Hoe-Han
authors.initials: HH
authors.lastname: Goh
authors.affiliation: Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia Bangi, Malaysia.
authors.forename: Normah M
authors.initials: NM
authors.lastname: Noor
keywords: Illumina sequencing; Persicaria minor; de novo transcriptome; differentially expressed gene; elicitation