title: In vivo near-infrared fluorescence imaging of amyloid-β plaques with a dicyanoisophorone-based probe.
abstract: A dicyanoisophorone-based probe with two-photon absorption and NIR emission was developed for the in vivo fluorescence imaging of amyloid-β plaques, which exhibited high selectivity toward Aβ aggregates over other intracellular proteins. The detection limit was calculated to be as low as 109 nM. In vivo imaging studies indicated that the probe could penetrate the blood-brain barrier and label Aβ plaques in the living transgenic mice, and its specific binding to cerebral Aβ plaques was further confirmed by one- and two-photon ex vivo fluorescence imaging. All these results featured its promising application prospects for amyloid-β sensing in basic research and biomedical research.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, PR China.
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, PR China.
authors.forename: Lin-Fu
authors.initials: LF
authors.lastname: Zhou
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, PR China.
authors.forename: Yu-Kun
authors.initials: YK
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, 510006, PR China.
authors.forename: Shuo-Bin
authors.initials: SB
authors.lastname: Chen
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, PR China. Electronic address: yjw@scut.edu.cn.
authors.forename: Jin-Wu
authors.initials: JW
authors.lastname: Yan
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, PR China. Electronic address: lzhangce@scut.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000544:Alzheimer Disease / Q000000981:diagnostic imaging / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000473:pathology; D005456:Fluorescent Dyes; D051379:Mice; D008822:Mice, Transgenic; D061848:Optical Imaging
chemical_list: D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes
keywords: Alzheimer's disease; Amyloid-β plaque; Bioimaging; Fluorescent; Near-infrared