title: Pie waste - A component of food waste and a renewable substrate for producing ethanol.
abstract: Sugar-rich food waste is a sustainable feedstock that can be converted into ethanol without an expensive thermochemical pretreatment that is commonly used in first and second generation processes. In this manuscript we have outlined the pie waste conversion to ethanol through a two-step process, namely, enzyme hydrolysis using commercial enzyme products mixtures and microbial fermentation using yeast. Optimized enzyme cocktail was found to be 45% alpha amylase, 45% gamma amylase, and 10% pectinase at 2.5mg enzyme protein/g glucan produced a hydrolysate with high glucose concentration. All three solid loadings (20%, 30%, and 40%) produced sugar-rich hydrolysates and ethanol with little to no enzyme or yeast inhibition. Enzymatic hydrolysis and fermentation process mass balance was carried out using pie waste on a 1000g dry weight basis that produced 329g ethanol at 20% solids loading. This process clearly demonstrate how food waste could be efficiently converted to ethanol that could be used for making biodiesel by reacting with waste cooking oil.
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Material Science, Michigan State University (MSU), 3900 Collins Road, MBI Building, Lansing, MI 48910, United States.
authors.forename: Margaret
authors.initials: M
authors.lastname: Magyar
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Material Science, Michigan State University (MSU), 3900 Collins Road, MBI Building, Lansing, MI 48910, United States.
authors.forename: Leonardo
authors.initials: L
authors.lastname: da Costa Sousa
authors.affiliation: Government College of Technology, Coimbatore 641013, Tamil Nadu, India.
authors.forename: Singaram
authors.initials: S
authors.lastname: Jayanthi
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Material Science, Michigan State University (MSU), 3900 Collins Road, MBI Building, Lansing, MI 48910, United States. Electronic address: balan@msu.edu.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balan
mesh_terms: D002241:Carbohydrates; D000431:Ethanol; D005285:Fermentation; D005502:Food; D005735:Garbage; D006868:Hydrolysis; D012037:Refuse Disposal / Q000379:methods*; D012441:Saccharomyces cerevisiae
chemical_list: D002241:Carbohydrates; D000431:Ethanol
keywords: Bio-based enzymatic hydrolysis; Biofuels; Ethanol; Food waste bioconversion; Microbial fermentation