title: Three odorant binding proteins may regulate the behavioural response of Chrysopa pallens to plant volatiles and the aphid alarm pheromone (E)-β-farnesene.
abstract: Artificial Chrysopa pallens release is a well-known method for suppressing aphids, but it is difficult to establish lacewing populations in the field. Understanding the functions of C. pallens odorant-binding proteins (CpalOBPs) and behavioural responses of C. pallens to plant volatiles and aphid alarm pheromone (E)-ß-farnesene has important implications for population establishment after lacewing release. Based on our previous study, five antennae-enriched CpalOBPs were selected. Sequence alignment and phylogenetic analysis revealed that these five CpalOBPs were Classic OBPs and separated into different clades. Of them, CpalOBP10 clustered in the same clade with aphid OBP7, which mediates the perception of green leaf volatiles and (E)-ß-farnesene. Ligand-binding assays showed 31 compounds, including plant-derived compounds, pest-induced volatiles and (E)-ß-farnesene, had high binding affinities for at least one of these five CpalOBPs. Of the 31 compounds, the pest-induced volatiles (Z)-3-hexenyl hexanoate and 2-hexyl-1-decanol, used in host location by the black bean aphid, elicited significant attractive behavioural responses from C. pallens. Conversely, (E)-ß-farnesene elicited strongly repellent behavioural responses. It is conceivable that C. pallens utilizes plant-derived compounds, pest-induced volatiles and (E)-ß-farnesene as foraging cues. Our studies provide new insights into the interrelationships amongst C. pallens, its prey and the host plants. Compounds that elicited significant behavioural responses from C. pallens were also identified.
authors.affiliation: Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, China.
authors.forename: Z-Q
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, China.
authors.forename: X-M
authors.initials: XM
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Luo
authors.affiliation: College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China.
authors.forename: S-L
authors.initials: SL
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Cui
authors.affiliation: Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, China.
authors.forename: Z-M
authors.initials: ZM
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids; D007300:Insect Control; D007313:Insecta / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D010675:Pheromones; D010944:Plants / Q000737:chemistry; D018035:Receptors, Odorant / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012717:Sesquiterpenes / Q000494:pharmacology*; D055549:Volatile Organic Compounds / Q000494:pharmacology*
chemical_list: D010675:Pheromones; D018035:Receptors, Odorant; D012717:Sesquiterpenes; D055549:Volatile Organic Compounds; C045648:odorant-binding protein; C062671:beta-farnesene
keywords: Chrysopa pallens; behavioural response; fluorescence binding assay; odorant-binding protein