title: Does metabolite channeling accelerate enzyme-catalyzed cascade reactions?
abstract: Metabolite or substrate channeling is a direct transfer of metabolites from one enzyme to the next enzyme in a cascade. Among many potential advantages of substrate channeling, acceleration of the total reaction rate is considered as one of the most important and self-evident. However, using a simple model, supported by stochastic simulations, we show that it is not always the case; particularly at long times (i.e. in steady state) and high substrate concentrations, a channeled reaction cannot be faster, and can even be slower, than the original non-channeled cascade reaction. In addition we show that increasing the degree of channeling may lead to an increase of the metabolite pool size. We substantiate that the main advantage of channeling likely lies in protecting metabolites from degradation or competing side reactions.
authors.affiliation: Forschungszentrum Jülich, IBG-1: Biotechnology, Jülich, Germany.|Forschungszentrum Jülich, IEK-7: Institute of Energy and Climate Research, Jülich, Germany.
authors.forename: Liubov
authors.initials: L
authors.lastname: Poshyvailo
authors.affiliation: Forschungszentrum Jülich, IBG-1: Biotechnology, Jülich, Germany.
authors.forename: Eric
authors.initials: E
authors.lastname: von Lieres
authors.affiliation: Forschungszentrum Jülich, IBG-1: Biotechnology, Jülich, Germany.
authors.forename: Svyatoslav
authors.identifier: http://orcid.org/0000-0003-4448-0686
authors.initials: S
authors.lastname: Kondrat
mesh_terms: D000465:Algorithms; D055162:Biocatalysis; D004798:Enzymes / Q000737:chemistry*; D007700:Kinetics; D013269:Stochastic Processes; D013379:Substrate Specificity
chemical_list: D004798:Enzymes