title: Biomarkers of Ionizing Radiation Exposure: A Multiparametric Approach.
abstract: Humans are exposed to ionizing radiation not only through background radiation but also through the ubiquitous presence of devices and sources that generate radiation. With the expanded use of radiation in day-to-day life, the chances of accidents or misuse only increase. Therefore, a thorough understanding of the dynamic effects of radiation exposure on biological entities is necessary. The biological effects of radiation exposure on human cells depend on much variability such as level of exposure, dose rate, and the physiological state of the cells. During potential scenarios of a large-scale radiological event which results in mass casualties, dose estimates are essential to assign medical attention according to individual needs. Many attempts have been made to identify biomarkers which can be used for high throughput biodosimetry screening. In this study, we compare the results of different biodosimetry methods on the same irradiated cells to assess the suitability of current biomarkers and push forward the idea of employing a multiparametric approach to achieve an accurate dose and risk estimation.
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Dimphy
authors.initials: D
authors.lastname: Zeegers
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Shriram
authors.initials: S
authors.lastname: Venkatesan
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Shu Wen
authors.initials: SW
authors.lastname: Koh
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Grace Kah Mun
authors.initials: GK
authors.lastname: Low
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Pallavee
authors.initials: P
authors.lastname: Srivastava
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Neisha
authors.initials: N
authors.lastname: Sundaram
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; GROW Research Laboratory, Narayana Nethralaya Foundation, Bangalore, Karnataka, India.
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethu
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India.
authors.forename: Birendranath
authors.initials: B
authors.lastname: Banerjee
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; NanoString Technologies, Seattle, WA, USA.
authors.forename: Manikandan
authors.initials: M
authors.lastname: Jayapal
authors.affiliation: Division of Human Health, International Atomic Energy Agency, Vienna, Austria.
authors.forename: Oleg
authors.initials: O
authors.lastname: Belyakov
authors.affiliation: Research Office, Sengkang Health, Alexandra Hospital, Singapore.
authors.forename: Rajamanickam
authors.initials: R
authors.lastname: Baskar
authors.affiliation: REAC/TS, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, Oak Ridge TN, USA.
authors.forename: Adayabalam S
authors.initials: AS
authors.lastname: Balajee
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Division of Human Health, International Atomic Energy Agency, Vienna, Austria; Tembusu College, National University of Singapore, Singapore.
authors.forename: M Prakash
authors.initials: MP
authors.lastname: Hande
keywords: dicentrics; gene signatures; micronuclei; multiparametric approach; radiation biodosimetry; translocations; γH2AX