title: Endophytic fungus Purpureocillium sp. A5 protect mangrove plant Kandelia candel under copper stress.
abstract: Mangrove is an important ecosystem in the world. Mangrove ecosystems have a large capacity in retaining heavy metals, and now they are usually considered as sinks for heavy metals. However, the mechanism of why the soil of mangrove ecosystems can retain heavy metal is not certain. In this research, endophytic fungus Purpureocillium sp. A5 was isolated and identified from the roots of Kandelia candel. When this fungus was added, it protected the growth of K. candel under Cu stress. This can be illustrated by analyzing chlorophyll A and B, RWC and WSD to leaves of K. candel. Purpureocillium sp. A5 reduces uptake of Cu in K. candel and changes the pH characterization of soil. Furthermore, A5 increase the concentration of Cu complexes in soil, and it enhanced the concentration of carbonate-bound Cu, Mn-Fe complexes Cu and organic-bound Cu in soil. Nevertheless, a significant reduction of the Cu ion was noted among A5-treated plants. This study is significant and illustrates a promising potential use for environmental remediation of endophytes, and also may partially explain the large capacity of mangrove ecosystems in retaining heavy metals.
authors.affiliation: Qinzhou University, Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Guangxi, China. Electronic address: 342965691@aliyun.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Gong
authors.affiliation: Qinzhou University, Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Guangxi, China.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Qinzhou University, Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Guangxi, China.
authors.forename: Rquan
authors.initials: R
authors.lastname: Liao
authors.affiliation: Qinzhou University, Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Guangxi, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Song
authors.affiliation: Qinzhou University, Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Guangxi, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D003300:Copper / Q000032:analysis / Q000378:metabolism*; D060026:Endophytes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006999:Hypocreales / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D043982:Rhizophoraceae / Q000378:metabolism* / Q000382:microbiology*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D012987:Soil; D003300:Copper
keywords: Copper stress; Endophytic fungi; Kandelia candel; Mangrove; Purpureocillium lilacinum