title: Production of Cinnamyl Alcohol Glucoside from Glucose in Escherichia coli.
abstract: Rosin, a cinnamyl alcohol glucoside, is one of the important ingredients in Rhodiola rosea, which is a valuable medicinal herb used for centuries. Rosin displayed multiple biological activities. The traditional method for producing rosin and derivatives is direct extraction from R. rosea, which suffers from limited availability of natural resources and complicated purification procedure. This work achieved de novo biosynthesis of rosin in Escherichia coli. First, a biosynthetic pathway of aglycon cinnamyl alcohol from phenylalanine was constructed. Subsequently, the UGT genes from Rhodiola sachalinensis (UGT73B6) or Arabidopsis thaliana (UGT73C5) were introduced into the above recombinant E. coli strain to produce rosin. Then the phenylalanine metabolic pathway of E. coli was optimized by genetic manipulation, and the production of rosin by the engineered E. coli reached 258.5 ± 8.8 mg/L. This study lays a significant foundation for microbial production of rosin and its derivatives using glucose as the renewable carbon source.
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.|University of Chinese Academy of Sciences , Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Bi
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: He
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Tao
authors.identifier: http://orcid.org/0000-0002-7076-1273
authors.initials: T
authors.lastname: Liu
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D020005:Propanols / Q000378:metabolism*
chemical_list: D005960:Glucosides; D020005:Propanols; D010649:Phenylalanine; D005947:Glucose; C020722:cinnamyl alcohol
keywords: E. coli; Rhodiola rosea; UGT73B6; UGT73C5; biosynthesis; cinnamyl alcohol; rosin