title: Sevoflurane pretreatment inhibits the myocardial apoptosis caused by hypoxia reoxygenation through AMPK pathway: An experimental study.
abstract: OBJECTIVE
  To study whether sevoflurane pretreatment inhibits the myocardial apoptosis caused by hypoxia reoxygenation through AMPK pathway.
  
  
  METHODS
  H9c2 myocardial cell lines were cultured and divided into control group (C group), hypoxia reoxygenation group (H/R group), sevoflurane pretreatment + hypoxia reoxygenation group (SP group) and sevoflurane combined with Compound C pretreatment + hypoxia reoxygenation group (ComC group), and the cell proliferation activity and apoptosis rate, myocardial enzyme levels in culture medium as well as the expression of apoptosis genes and p-AMPK in cells were determined.
  
  
  RESULTS
  p-AMPK expression in cells of H/R group was significantly lower than that of C group, SP group was significantly higher than that of H/R group; cell proliferation activity value and Bcl-2 expression in cells of H/R group were significantly lower than those of C group, SP group were significantly higher than those of H/R group, ComC group were significantly lower than those of SP group; apoptosis rate, LDH, CK and AST levels as well as the Bax and Caspase-3 expression in cells of H/R group were significantly higher than those of C group, SP group were significantly lower than those of H/R group, ComC group were significantly higher than those of SP group.
  
  
  CONCLUSIONS
  Sevoflurane pretreatment can activate AMPK signaling pathway to inhibit the myocardial apoptosis caused by hypoxia reoxygenation.
authors.affiliation: Department of Cardiology, Linyi People's Hospital in Shandong Province, Linyi City 276000, Shandong Province, China.
authors.forename: Zhao-Ying
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Linyi People's Hospital in Shandong Province, Linyi City 276000, Shandong Province, China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Hu
authors.affiliation: Department of Cardiology, Linyi People's Hospital in Shandong Province, Linyi City 276000, Shandong Province, China. Electronic address: jsensing@163.com.
authors.forename: Qing-Rong
authors.initials: QR
authors.lastname: Ji
authors.affiliation: Department of Cardiology, Linyi People's Hospital in Shandong Province, Linyi City 276000, Shandong Province, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, Linyi People's Hospital in Shandong Province, Linyi City 276000, Shandong Province, China.
authors.forename: Dong-Hao
authors.initials: DH
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Linyi People's Hospital in Shandong Province, Linyi City 276000, Shandong Province, China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Wu
keywords: Adenosine; Apoptosis; Monophosphate-activated; Myocardial hypoxia reoxygenation; Protein kinase; Sevoflurane