title: Monitoring Mitophagy in Mammalian Cells.
abstract: In eukaryotes, physiological cell functions rely on the preservation of the size and activity of the mitochondrial network. Mitophagy provides a key contribution in this setting by ensuring the removal of permeabilized or supernumerary mitochondria. Throughout the past decade, mitophagy has attracted considerable attention from both fundamental researchers and translational investigators, both of whom have called for the development of techniques that allow the precise quantification of mitophagy (as opposed to general autophagy). In this chapter, we present morphological, biochemical, and fluorescence-based approaches to measure autophagy in mammalian cells, and discuss recent progress in mouse models for the assessment of mitophagy in vivo.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: K
authors.initials: K
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: J
authors.initials: J
authors.lastname: Han
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Electronic address: chenq@ioz.ac.cn.
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, China. Electronic address: zhuys@nankai.edu.cn.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D063306:Mitophagy; D019076:Transgenes
chemical_list: D005456:Fluorescent Dyes; D008869:Microtubule-Associated Proteins; D049452:Green Fluorescent Proteins
keywords: Autophagy; Electron microscopy; LC3; Mitophagy; Western blotting