title: Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells.
abstract: Appropriate cell models are necessary for the investigation of thermogenic beige adipocyte differentiation from progenitor cells. Here, we describe a primary cell culture method that is based on defined progenitor cells from murine white adipose tissue and aims at minimizing confounding factors including cell heterogeneity and nonphysiological differentiation inducers. Adipocyte progenitor cells are enriched by immuno-magnetic separation, expanded minimally, and induced for beige adipocyte differentiation with carbaprostacyclin, a stable analogue of the endogenous mediator PGI2.
authors.affiliation: DKFZ Junior Group Metabolism and Stem Cell Plasticity (A171), German Cancer Research Center, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany.
authors.forename: Rohollah
authors.initials: R
authors.lastname: Babaei
authors.affiliation: DKFZ Junior Group Metabolism and Stem Cell Plasticity (A171), German Cancer Research Center, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany.
authors.forename: Irem
authors.initials: I
authors.lastname: Bayindir-Buchhalter
authors.affiliation: DKFZ Junior Group Metabolism and Stem Cell Plasticity (A171), German Cancer Research Center, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Meln
authors.affiliation: DKFZ Junior Group Metabolism and Stem Cell Plasticity (A171), German Cancer Research Center, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany. a.vegiopoulos@dkfz.de.
authors.forename: Alexandros
authors.initials: A
authors.lastname: Vegiopoulos
mesh_terms: D052437:Adipocytes, Brown / Q000166:cytology / Q000378:metabolism; D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism; D052436:Adipose Tissue, White / Q000166:cytology*; D000818:Animals; D000943:Antigens, Differentiation; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D018189:Immunomagnetic Separation / Q000379:methods; D016130:Immunophenotyping / Q000379:methods; D051379:Mice; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D000943:Antigens, Differentiation
keywords: Adipocyte progenitors; Adipogenic differentiation; Beige adipocytes; Browning; Carbaprostacyclin; Primary cell isolation; Thermogenic adipocytes; cPGI2