title: Two-photon laser-scanning microscopy for single and repetitive imaging of dorsal and lateral spinal white matter in vivo.
abstract: We developed appropriate surgical procedures for single and repetitive multi-photon imaging of spinal cord in vivo. By intravenous anesthesia, artificial ventilation and laminectomy, acute experiments were performed in the dorsal and lateral white matter. By volatile anesthesia and minimal-invasive surgery, chronic repetitive imaging up to 8 months were performed in the dorsal column through the window between two adjacent spines. Transgenic mouse technology enabled simultaneous imaging of labeled axons, astrocytes and microglia. Repetitive imaging showed positional shifts of microglia over time. These techniques serve for investigations of cellular dynamics and cell-cell interactions in intact and pathologically changed spinal tissue.
authors.affiliation: Max-Planck-Institute for Experimental Medicine, Göttingen, Germany. p_dibaj@yahoo.com.
authors.forename: F
authors.initials: F
authors.lastname: Nadrigny
authors.forename: K
authors.initials: K
authors.lastname: Le Meur
authors.forename: E D
authors.initials: ED
authors.lastname: Schomburg
authors.forename: S
authors.initials: S
authors.lastname: Safavi-Abbasi
authors.forename: P
authors.initials: P
authors.lastname: Dibaj
mesh_terms: D000818:Animals; D047569:Laser Scanning Cytometry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D009924:Organ Culture Techniques; D013116:Spinal Cord / Q000166:cytology* / Q000000981:diagnostic imaging*; D066127:White Matter / Q000166:cytology* / Q000000981:diagnostic imaging*