title: Construction of new EST-SSRs for Fusarium resistant wheat breeding.
abstract: Surveying Fusarium resistance in wheat with easy applicable molecular markers such as simple sequence repeats (SSRs) is a prerequest for molecular breeding. Expressed sequence tags (ESTs) are one of the main sources for development of new SSR candidates. Therefore, 18.292 publicly available wheat ESTs were mined and genotyping of newly developed 55 EST-SSR derived primer pairs produced clear fragments in ten wheat cultivars carrying different levels of Fusarium resistance. Among the proved markers, 23 polymorphic EST-SSRs were obtained and related alleles were mostly found on B and D genome. Based on the fragment profiling and similarity analysis, a 327bp amplicon, which was a product of contig 1207 (chromosome 5BL), was detected only in Fusarium head blight (FHB) resistant cultivars (CM82036 and Sumai) and the amino acid sequences showed a similarity to pathogen related proteins. Another FHB resistance related EST-SSR, Contig 556 (chromosome 1BL) produced a 151bp fragment in Sumai and was associated to wax2-like protein. A polymorphic 204bp fragment, derived from Contig 578 (chromosome 1DL), was generated from root rot (FRR) resistant cultivars (2-49; Altay2000 and Sunco). A total of 98 alleles were displayed with an average of 1.8 alleles per locus and the polymorphic information content (PIC) ranged from 0.11 to 0.78. Dendrogram tree with two main and five sub-groups were displayed the highest genetic relationship between FRR resistant cultivars (2-49 and Altay2000), FRR sensitive cultivars (Seri82 and Scout66) and FHB resistant cultivars (CM82036 and Sumai). Thus, exploitation of these candidate EST-SSRs may help to genotype other wheat sources for Fusarium resistance.
authors.affiliation: University of Marmara, Faculty of Science and Letters, Department of Biology, 34722 İstanbul, Turkey. Electronic address: yumurtaciay@yahoo.com.
authors.forename: Aysen
authors.initials: A
authors.lastname: Yumurtaci
authors.affiliation: University of Sinop, Faculty of Arts and Sciences, Department of Biology, 57000 Sinop, Turkey. Electronic address: hulyasipahi@hotmail.com.
authors.forename: Hulya
authors.initials: H
authors.lastname: Sipahi
authors.affiliation: International Center for Agricultural Research in the Dry Areas (ICARDA), Giza, Egypt; University of Jordan, Faculty of Agriculture, Department of Horticulture and Crop Science, 11942 Amman, Jordan. Electronic address: a.al-abdallat@cgiar.org.
authors.forename: Ayed
authors.initials: A
authors.lastname: Al-Abdallat
authors.affiliation: International Center for Agricultural Research in the Dry Areas (ICARDA), Giza, Egypt.
authors.forename: Abdulqader
authors.initials: A
authors.lastname: Jighly
authors.affiliation: International Center for Agricultural Research in the Dry Areas (ICARDA), Av. Mohamed Belarbi Alaoui, PO Box 6299, Al Irfane, Rabat, Morocco. Electronic address: m.baum@cgiar.org.
authors.forename: Michael
authors.initials: M
authors.lastname: Baum
mesh_terms: D020224:Expressed Sequence Tags; D005670:Fusarium / Q000472:pathogenicity / Q000502:physiology*; D018895:Microsatellite Repeats; D000069600:Plant Breeding; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
keywords: EST; Fusarium; Genetic diversity; Resistance; SSRs; Wheat