title: Detection of inherited chromosomally integrated HHV-6 (ciHHV-6) in a marker chromosome.
abstract: OBJECTIVE
  Inherited chromosomally integrated human herpesvirus-6 (ciHHV-6) is characterised by the complete HHV-6 genome integration into the host germ line genome and is vertically transmitted with a Mendelian inheritance. By now, the only relationship between ciHHV-6 and diseases seems to be with angina pectoris.
  
  
  METHODS
  We report a case of an 82-year-old man diagnosed with diffuse large B-cell lymphoma (DLBCL) on October 2014. To substantiate the suspicion of ciHHV-6, we analysed peripheral blood mononuclear cells, bone marrow biopsy and pleural effusion-derived mesothelial cells with PCR, RT-PCR and FISH.
  
  
  RESULTS
  Virological routine screening by PCR showed the absence of HHV-8 and EBV infections, while the presence of HHV-6 DNA (ie, U22, U42 and U94 HHV-6 genes), with a viral load of about 1.0 genome per cell, strongly suggests ciHHV-6. The RT-PCR showed the positivity only for the immediate-early U94, at low levels of transcription (100±15 transcripts/1 μg RNA). FISH analysis reported a case of inherited ciHHV-6 in 17p chromosome region and, for the first time, in a marker chromosome.
  
  
  CONCLUSIONS
  This is the first case of inherited ciHHV-6 in a marker chromosome, possibly elucidating the role of this abnormality in the biology of DLBCL.
authors.affiliation: Section of Microbiology, University of Ferrara, Ferrara, Italy.
authors.forename: Diana
authors.initials: D
authors.lastname: Campioni
authors.affiliation: Section of Haematology, University of Ferrara, Ferrara, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Gentili
authors.affiliation: Section of Microbiology, University of Ferrara, Ferrara, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cavazzini
authors.affiliation: Section of Haematology, University of Ferrara, Ferrara, Italy.
authors.forename: Daria
authors.initials: D
authors.lastname: Bortolotti
authors.affiliation: Department of Haematology, Royal Free & University College Medical School, London, UK.
authors.forename: Elisabeth P
authors.initials: EP
authors.lastname: Nacheva
authors.affiliation: Section of Microbiology, University of Ferrara, Ferrara, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cuneo
authors.affiliation: Section of Haematology, University of Ferrara, Ferrara, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Di Luca
authors.affiliation: Section of Haematology, University of Ferrara, Ferrara, Italy.
authors.forename: Roberta
authors.identifier: http://orcid.org/0000-0001-9507-9126
authors.initials: R
authors.lastname: Rizzo
mesh_terms: D000369:Aged, 80 and over; D002886:Chromosomes, Human, Pair 17 / Q000737:chemistry*; D015870:Gene Expression; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D040582:Inheritance Patterns; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism
chemical_list: D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: ciHHV-6; diffuse large B-cell lymphoma; genome integration; marker chromosome; non-Hodgkin's lymphomas