title: [Establishment of a Xenografted Acute Myeloid Leukemia Model by using Zebrafish].
abstract: Objectve: To investigate the feasibility of establishing xenografted leukemia model by zebrafish, so as to provide the more direct model in vitro and experimental evidence for study of acute myeloid leukemia and screening of the drugs for targeting therapy.
  
  
  METHODS
  Acute myeloid leukemia cell line KG-1a was labeled with red fluorescent dye-MitoRed, then the labeled cells were injected into the yolk sac of zebrafish embryos. Morphological observation, cell count and histopathological detection were used to analyse the infiltration and metastasis of KG-1a cells in zebrafish.
  
  
  RESULTS
  KG1a cells could proliferate and gradually spread to the entire abdominal cavity of the zebrafish after KG-1a cells were injected into the yolk sac during 1-7, the results of cell counting in vitro also proved a significant proliferation of KG-1a cells in zebrafish, suggesting that the implanted leukemia stem cells could survive, proliferate and spread in zebrafish. Further study showed that the implanted cells could be transfered to the liver of zebrafish, these cells displayed the signature of KG-1a cells by hematoxylin-eosin(HE) staining.
  
  
  CONCLUSIONS
  Human acute myeloid leukemia cells KG1a can survive, proliferate and migrate in zebrafish, suggesting xenografted leukemia model of zebrafish has been successfully established. This model may be benefitcial for the study of acute myeloid leukemia and the screening of the drugs for targeting therapy of acute myeloid leukemia.
authors.affiliation: Department of Hematology; Zhongshan Hospital Affiliated to Sun Yat-Sen University, Zhongshan 528400, China.
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Ye
authors.affiliation: Department of Hematology; Zhongshan Hospital Affiliated to Sun Yat-Sen University, Zhongshan 528400, China.
authors.forename: Ming-Wan
authors.initials: MW
authors.lastname: Zhang
authors.affiliation: Department of Hematology; Zhongshan Hospital Affiliated to Sun Yat-Sen University, Zhongshan 528400, China.
authors.forename: Da-Fa
authors.initials: DF
authors.lastname: Qiu
authors.affiliation: Department of Hematology; Zhongshan Hospital Affiliated to Sun Yat-Sen University, Zhongshan 528400, China.
authors.forename: Zi-Wen
authors.initials: ZW
authors.lastname: Guo
authors.affiliation: Department of Hematology; Zhongshan Hospital Affiliated to Sun Yat-Sen University, Zhongshan 528400, China.
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: He
authors.affiliation: Department of Hematology; Zhongshan Hospital Affiliated to Sun Yat-Sen University, Zhongshan 528400, China. E-mail: doctorxu@163.com.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Xu
mesh_terms: D000818:Animals; D002452:Cell Count; D004195:Disease Models, Animal; D064593:Heterografts; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D015027:Zebrafish