title: Novel biotechnology approaches in colorectal cancer diagnosis and therapy.
abstract: With ever-increasing molecular information about colorectal cancer (CRC), there is an expectation to detect more sensitive and specific molecular markers for new advanced diagnostic methods that can surpass the limitations of current screening tests. Moreover, enhanced molecular pathology knowledge about cancer has led to the development of targeted therapies, designed to interfere with specific aberrant biological pathways in cancer. Furthermore, biotechnology has opened a new window in CRC diagnosis and treatment by introducing different application of antibodies, antibody fragments, non-Ig scaffold proteins, and aptamers in targeted therapy and drug delivery. This review summarizes the molecular diagnostic and therapeutic approaches in CRC with a focus on genetic and epigenetic alterations, protein and metabolite markers as well as targeted therapy and drug delivery by Ig-scaffold proteins, non-Ig scaffold proteins, nanobodies, and aptamers.
authors.affiliation: Department of Biotechnology, School of Advanced Medical Science and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Soudabeh
authors.initials: S
authors.lastname: Kavousipour
authors.affiliation: Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, P.O Box 1167, Shiraz, Iran.
authors.forename: Fathemeh
authors.initials: F
authors.lastname: Khademi
authors.affiliation: Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mozhdeh
authors.initials: M
authors.lastname: Zamani
authors.affiliation: Department of Biotechnology, School of Advanced Medical Science and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Vakili
authors.affiliation: Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, P.O Box 1167, Shiraz, Iran. mokaram2@gmail.com.|Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. mokaram2@gmail.com.|School of Medicine, Nemazee Hospital, Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. mokaram2@gmail.com.
authors.forename: Pooneh
authors.initials: P
authors.lastname: Mokarram
mesh_terms: D001709:Biotechnology / Q000379:methods*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000628:therapy; D016503:Drug Delivery Systems; D006801:Humans; D025202:Molecular Diagnostic Techniques
keywords: Colorectal cancer; Fragment antibody; Scaffold proteins; Targeted therapy