title: RD26 mediates crosstalk between drought and brassinosteroid signalling pathways.
abstract: Brassinosteroids (BRs) regulate plant growth and stress responses via the BES1/BZR1 family of transcription factors, which regulate the expression of thousands of downstream genes. BRs are involved in the response to drought, however the mechanistic understanding of interactions between BR signalling and drought response remains to be established. Here we show that transcription factor RD26 mediates crosstalk between drought and BR signalling. When overexpressed, BES1 target gene RD26 can inhibit BR-regulated growth. Global gene expression studies suggest that RD26 can act antagonistically to BR to regulate the expression of a subset of BES1-regulated genes, thereby inhibiting BR function. We show that RD26 can interact with BES1 protein and antagonize BES1 transcriptional activity on BR-regulated genes and that BR signalling can also repress expression of RD26 and its homologues and inhibit drought responses. Our results thus reveal a mechanism coordinating plant growth and drought tolerance.
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Huaxun
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA.|Data2Bio LLC, Ames, Iowa 50011-3650, USA.
authors.forename: Sanzhen
authors.identifier: 0000-0002-9513-855X
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Buyun
authors.initials: B
authors.lastname: Tang
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Jiani
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Zhouli
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Trevor M
authors.initials: TM
authors.lastname: Nolan
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Hongqing
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Hung-Ying
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Hongning
authors.initials: H
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, Department of Agronomy, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Mingcai
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Chengcai
authors.identifier: 0000-0001-8097-6115
authors.initials: C
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, Department of Agronomy, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Zhaohu
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: Maneesha
authors.initials: M
authors.lastname: Aluru
authors.affiliation: School of Computational Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Aluru
authors.affiliation: Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA.|Data2Bio LLC, Ames, Iowa 50011-3650, USA.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Schnable
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060406:Brassinosteroids / Q000378:metabolism*; D004268:DNA-Binding Proteins; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D000073658:Loss of Function Mutation; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D010937:Plant Growth Regulators / Q000502:physiology*; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C456160:BES1 protein, Arabidopsis; D060406:Brassinosteroids; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010937:Plant Growth Regulators; C493887:RD26 protein, Arabidopsis; D014157:Transcription Factors