title: [Effects of TRAM-34 on Proliferation and Invasion of Leukemia Cell Line HL-60].
abstract: OBJECTIVE
  To investigate the effects of KCa3.1 channel inhibitor TRAM-34 on the proliferation and invasion of leukemia cell line HL-60.
  
  
  METHODS
  HL-60 cells at logarithmic growth phase exposed to TRAM-34 at the final concentration of 25, 50, 75 and 100 nmol/L were used as experimental group. The HL-60 cells of control group was cultured in 10% fetal bovine serum-RPMI 1640. The proliferation inhibition rate of TRAM-34 on HL-60 cells was detected by adding MTT solution after 24, 48 and 72 h culture. The cell apoptotic rate and cell cycle distribution of HL-60 cells treated with TRAM-34 were evaluated by flow cytometry with Annexin V-FITC/propidium iodide(PI) double staining or PI single staining. The number of transmembrane cells was detected by Transwell at 24 and 48 h after treatment with TRAM-34. The effect of TRAM-34 on CDK6, P53 and MMP-2 mRNA level was detected by real-time quantitative PCR.
  
  
  RESULTS
  Compared with the control group (0 nmol/L), the inhibition rate, apoptosis rate, G0/G1phase cell proportion and P53 mRNA level all increased, but the percentages of cells in S phase, cell number penetrating the membrane and mRNA levels of CDK6 and MMP-2 in the TRAM-34-treated group decreased (P<0.05) except for 24 h proliferation rate of TRAM-34 at low concentration (25 nmol/L). The effect of TRAM-34 on the above indices was enhanced with the increase of concentration and prolongation of time, and the differences were statistically significant (P<0.05).
  
  
  CONCLUSIONS
  TRAM-34 can inhibit the proliferation and invasion of HL-60 cells, and can induce cell apoptosis and G0/G1arrest. The time and concentration of TRAM-34 have effect on the malignant behavior of HL-60 cells.
authors.affiliation: Department of Blood Transfusion, Deyang Municipal People's Hospital of Sichuan Province, Deyang 618000, Sichuan Province, China.
authors.forename: Tian-Hua
authors.initials: TH
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Medicine in West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. E-mail: 1261369481@qq.com.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy*; D009361:Neoplasm Invasiveness; D011720:Pyrazoles / Q000494:pharmacology*
chemical_list: D011720:Pyrazoles; C411671:TRAM 34