title: Altered Plasma MicroRNA Expression in Patients with Mitral Chordae Tendineae Rupture.
abstract: Mitral chordae tendineae rupture (MCTR) is a progressive disorder which leads to severe mitral regurgitation. Despite its importance, the precise pathogenetic mechanism of MCTR remains unclear. The study aim was to investigate the expression profile of circulating microRNAs (miRNAs) as being potentially involved in the development of MCTR.
  
  
  
  Twenty-one patients with 'primary' MCTR, and 30 age- and gender-matched controls, were enrolled in the study. Comparisons were made between the expression levels of circulating miRNAs in MCTR patients and controls. Four target gene databases were used to predict target genes and pathways of differentially expressed miRNAs.
  
  
  
  Compared to controls, the expression of 22 miRNAs (hsa-miR-106b-5p, hsa-miR-126-3p, hsa-miR-150-5p, hsa-miR-17-5p, hsa-miR-195-5p, hsa-miR-19a-3p, hsa-miR-19b-3p, hsa-miR-20a-5p, hsa-miR-21-5p, hsa-miR-222-3p, hsa-miR-223-3p, hsa-miR-23a-3p, hsa-miR-25-3p, hsa-miR-92a-3p, hsa-miR-93-5p, hsa-miR-26b-5p, hsa-miR-30e-5p, hsa-miR-373-3p, hsa-miR-15b-5p, hsa-miR-16-5p, hsa-miR-191-5p, hsa-miR-26a-5p) were significantly down-regulated in the MCTR group. Bioinformatic analysis indicated that the following potential miRNA targets and pathways are commonly related to the development of MCTR: MMPs, TIMP-2,TGFBR2, VEGFA, PIK3R2, NRAS, PPP3CA, PPP3R1, PTGS 2 were predicted as putative targets of 13 of these miRNAs.
  
  
  
  The present study is the first to describe altered miRNA expression in patients with MCTR. Bioinformatic analysis has revealed that target genes involved in MCTR development were regulated by miRNAs.
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey. Electronic correspondence: bulentvatan@hotmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Bulent Vatan
authors.affiliation: Department of Medical Biology and Genetics, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Kalaycı Yigin
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Akdemir
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Tarik Agac
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Akif Cakar
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Aksoy
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Ersan
authors.initials: E
authors.lastname: Tatli
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Harun
authors.initials: H
authors.lastname: Kilic
authors.affiliation: Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Gunduz
authors.affiliation: Department of Physiology, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Guzel
authors.affiliation: Department of Anatomy, Sakarya University School of Medicine, Sakarya, Turkey.
authors.forename: Keziban
authors.initials: K
authors.lastname: Karacan
mesh_terms: D002815:Chordae Tendineae; D018076:DNA, Complementary / Q000096:biosynthesis; D005260:Female; D020022:Genetic Predisposition to Disease; D006349:Heart Valve Diseases / Q000097:blood* / Q000150:complications / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D060888:Real-Time Polymerase Chain Reaction; D012422:Rupture, Spontaneous / Q000097:blood / Q000150:complications / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs