title: [Effect of miR-705 on osteogenic differentiation of mouse embryo osteoblast precursor cells MC3T3-E1].
abstract: Objective:To investigate the effect of miR-705 on osteogenic differentiation of mouse embryo osteoblast precursor (MC3T3-E1) cells.Methods:miR-705 mimics, inhibitors and negative control were transfected into MC3T3-E1 cells. Alkaline phosphates (ALP) staining were performed and quantified after 7 days of osteogenic medium induction. The mRNA and protein expression levels of runt-related transcription factor 2 (Runx2) and osteocalcin (OCN) were detected by real-time RT-PCR and Western blot after 14 days of osteogenic induction. Alizarin red staining was performed and quantified in MC3T3-E1 cells after 21 days of osteogenic induction.Results:After 7 days of osteogenic induction, ALP staining showed that overexpression of miR-705 significantly reduced ALP activity, whereas knockdown of miR-705 increased ALP activity (allP<0.05). Consistently, after 14 days of osteogenic induction, mRNA and protein expressions of Runx2 and OCN were suppressed by overexpression of miR-705, whereas they were promoted by knockdown of miR-705 (allP<0.05). After 21 days of osteogenic induction, alizarin red staining showed that overexpression of miR-705 significantly reduced the formation of mineralized node, the opposite results were found in miR-705 knockdown group (allP<0.05).Conclusion:miR-705 can inhibit the osteogenic differentiation of MC3T3-E1 cells.
authors.affiliation: Department of Prosthetics, School of Stomatology, Zunyi Medical College, Zunyi 563000, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Periodontics, School of Stomatology, Zunyi Medical College, Zunyi 563000, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Military Stomatology, Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.affiliation: State Key Laboratory of Military Stomatology, Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an 710032, China. yanjin@fumm.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects / Q000235:genetics; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics*; D050659:Core Binding Factor Alpha 1 Subunit / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D053686:Fetal Stem Cells; D051379:Mice; D035683:MicroRNAs / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000187:drug effects / Q000235:genetics; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C000613310:MIRN705 microRNA, mouse; D035683:MicroRNAs; C493716:Runx2 protein, mouse; D015675:Osteocalcin; D000469:Alkaline Phosphatase