title: Rapid 3D Refractive-Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation.
abstract: A major challenge in the field of optical imaging of live cells is achieving rapid, 3D, and noninvasive imaging of isolated cells without labeling. If successful, many clinical procedures involving analysis and sorting of cells drawn from body fluids, including blood, can be significantly improved. A new label-free tomographic interferometry approach is presented. This approach provides rapid capturing of the 3D refractive-index distribution of single cells in suspension. The cells flow in a microfluidic channel, are trapped, and then rapidly rotated by dielectrophoretic forces in a noninvasive and precise manner. Interferometric projections of the rotated cell are acquired and processed into the cellular 3D refractive-index map. Uniquely, this approach provides full (360°) coverage of the rotation angular range around any axis, and knowledge on the viewing angle. The experimental demonstrations presented include 3D, label-free imaging of cancer cells and three types of white blood cells. This approach is expected to be useful for label-free cell sorting, as well as for detection and monitoring of pathological conditions resulting in cellular morphology changes or occurrence of specific cell types in blood or other body fluids.
authors.affiliation: Department of Biomedical Engineering Faculty of Engineering Tel Aviv University Tel Aviv 69978 Israel.
authors.forename: Mor
authors.initials: M
authors.lastname: Habaza
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology Branch Potsdam Am Muehlenberg 13 14476 Potsdam Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Kirschbaum
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology Branch Potsdam Am Muehlenberg 13 14476 Potsdam Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Guernth-Marschner
authors.affiliation: Department of Biomedical Engineering Faculty of Engineering Tel Aviv University Tel Aviv 69978 Israel.
authors.forename: Gili
authors.initials: G
authors.lastname: Dardikman
authors.affiliation: Department of Biomedical Engineering Faculty of Engineering Tel Aviv University Tel Aviv 69978 Israel; Department of Physiology and Pharmacology Faculty of Medicine Tel Aviv University Tel Aviv 69978 Israel.
authors.forename: Itay
authors.initials: I
authors.lastname: Barnea
authors.affiliation: Department of Physiology and Pharmacology Faculty of Medicine Tel Aviv University Tel Aviv 69978 Israel.
authors.forename: Rafi
authors.initials: R
authors.lastname: Korenstein
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology Branch Potsdam Am Muehlenberg 13 14476 Potsdam Germany.
authors.forename: Claus
authors.initials: C
authors.lastname: Duschl
authors.affiliation: Department of Biomedical Engineering Faculty of Engineering Tel Aviv University Tel Aviv 69978 Israel.
authors.forename: Natan T
authors.initials: NT
authors.lastname: Shaked
keywords: 3D cell imaging; digital holographic microscopy; microfluidics; quantitative phase microscopy; tomography