title: Anti-inflammatory ent-kaurenoic acids and their glycosides from Gochnatia decora.
abstract: Nineteen ent-kaurane diterpenes were isolated and identified from the barks of Gochnatia decora (Kurz) A. L. Cabrera (Compositae), which has been used as an ethnic medicine for treating cough, asthma and wounds in southwestern China. Among them, six compounds are previously undescribed ent-kaurenoic acids, and a known compound, 7β,15β-dihydroxy-ent-kaur-16-en-19-oic acid, was obtained for the first time from nature. Based on its traditional effects in Chinese folk, the potential anti-inflammatory activities of its methanol extracts (ME) and isolated diterpenes were evaluated by the tests of the xylene-induced ear swelling in mice, lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse macrophage cellular RAW 264.7 and inhibition assay of neutrophil elastase, respectively, resulting that ME performed obvious effect against mouse ear swelling with a dose-dependent inhibition in vivo, and nine compounds showed significant inhibition of NO production in vitro, with IC50values ranging from 0.042 to 8.22 μM, while they also exhibited inhibition of neutrophil elastase at 100 μM in vitro, speculating that those diterpenes may be the active substances correlated with their traditional efficacy.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Chenxing
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Dai
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
authors.forename: Shiyun
authors.initials: S
authors.lastname: Jiao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. Electronic address: baocaili@hotmail.com.
authors.forename: Baocai
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002681:China; D004224:Diterpenes / Q000302:isolation & purification / Q000493:pharmacokinetics*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors; D008297:Male; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D000067996:RAW 264.7 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D006027:Glycosides; D010936:Plant Extracts; D009569:Nitric Oxide; C051436:kaurenoic acid; D019272:Leukocyte Elastase
keywords: Anti-inflammatory; Compositae; Gochnatia decora; Neutrophil elastase inhibition; ent-kaurane