title: Effects of antifreeze glycoprotein 8 (AFGP8) supplementation during vitrification on the in vitro developmental capacity of expanded bovine blastocysts.
abstract: Cryopreservation is an effective method for the long-term storage of valuable germplasm in the field of reproductive research. The present study examined the developmental capacity of post-thaw bovine blastocysts during vitrification after supplementation with antifreeze glycoprotein 8 (AFGP8). Survival and re-expansion rates in culture during the 12h after thawing were significantly higher in the AFGP8-treated than untreated group. In addition, blastocysts from the AFGP8-treated group exhibited lower rates of apoptosis. Real-time reverse transcription-polymerase chain reaction analysis showed that the expression of the Bcl-2 gene, coding for an anti-apoptotic protein, was increased significantly, whereas the expression of the pro-apoptotic gene Bax was decreased significantly in the AFGP8-treated group. The cellular proliferation rate and mitochondrial membrane potential were significantly higher in post-thaw re-expanded blastocysts from the AFGP8-treated compared with untreated group. In addition, outgrowth potential in post-thaw blastocysts in re-expanded blastocysts after vitrification was significantly increased in the AFGP8-treated compared with untreated group. Together, these results are the first to demonstrate that the addition of AFGP8 during vitrification can help protect bovine blastocysts against chill-induced injury.
authors.affiliation: Department of Animal Science, Chungbuk National University, Naesudong-ro, Seowon-gu, Cheongju-si, Chungcheongbuk-do, 28864, Republic of Korea.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Animal Science, Chungbuk National University, Naesudong-ro, Seowon-gu, Cheongju-si, Chungcheongbuk-do, 28864, Republic of Korea.
authors.forename: Bao
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Xi'an Road 5333, Changchun, Jilin, 130062, China.
authors.forename: Yong-Xun
authors.initials: YX
authors.lastname: Jin
authors.affiliation: Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Xi'an Road 5333, Changchun, Jilin, 130062, China.
authors.forename: Jia-Bao
authors.initials: JB
authors.lastname: Zhang
authors.affiliation: Division of Magnetic Resonance, Korea Basic Science Institute, Ochang, Chung-Buk, 363-883, Republic of Korea.
authors.forename: Jeong Kyu
authors.initials: JK
authors.lastname: Bang
authors.affiliation: Department of Animal Science, Chungbuk National University, Naesudong-ro, Seowon-gu, Cheongju-si, Chungcheongbuk-do, 28864, Republic of Korea.
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
mesh_terms: D000818:Animals; D021301:Antifreeze Proteins / Q000008:administration & dosage*; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D058989:Vitrification
chemical_list: D021301:Antifreeze Proteins; D003451:Cryoprotective Agents