title: LeEIX2 Interactors' Analysis and EIX-Mediated Responses Measurement.
abstract: Plant-pathogen interactions involve a large number of wide regulatory systems, necessary for plant defense responses against pathogen attack. The fungal protein ethylene-inducing xylanase (EIX) elicits defense responses in specific cultivars of tobacco and tomato. The response to EIX is controlled by a single locus encoding for LeEIX2, a leucine-rich-repeat receptor-like-protein (LRR-RLP). As an RLP, LeEIX2 does not possess an obvious cytoplasmic signaling moiety such as a kinase domain. To study LeEIX2 mode of action, it is essential to identify the potential interactors involved after EIX perception. Here, we describe the in vivo co-IP methodology used for protein interaction verification and ethylene and ROS (reactive oxygen species) measurements used for physiological effects assessment.
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Tel-Aviv University, Tel-Aviv, 69978, Israel.
authors.forename: Meirav
authors.initials: M
authors.lastname: Leibman-Markus
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Tel-Aviv University, Tel-Aviv, 69978, Israel.
authors.forename: Silvia
authors.initials: S
authors.lastname: Schuster
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Tel-Aviv University, Tel-Aviv, 69978, Israel. lpavni@post.tau.ac.il.
authors.forename: Adi
authors.initials: A
authors.lastname: Avni
mesh_terms: D005030:Ethylenes / Q000032:analysis; D005656:Fungal Proteins / Q000276:immunology* / Q000378:metabolism; D047468:Immunoprecipitation; D018551:Solanum lycopersicum / Q000276:immunology* / Q000378:metabolism; D057865:Plant Immunity; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains; D017382:Reactive Oxygen Species / Q000032:analysis; D015398:Signal Transduction; D014026:Nicotiana / Q000276:immunology* / Q000378:metabolism
chemical_list: D005030:Ethylenes; D005656:Fungal Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C036216:ethylene
keywords: Co-IP; Defense responses; Ethylene-inducing xylanase; LRR-RLP; MAMP; ROS