title: Characterization of peptide attachment on silicon nanowires by X-ray photoelectron spectroscopy and mass spectrometry.
abstract: In this paper, we report an original method to immobilize a model peptide on silicon nanowires (SiNWs) via a photolinker attached to the SiNWs' surface. The silicon nanowires were fabricated by a metal assisted chemical etching (MACE) method. Then, direct characterization of the peptide immobilization on SiNWs was performed either by X-ray photoelectron spectroscopy (XPS) or by laser-desorption/ionization mass spectrometry (LDI-MS). XPS allowed us to follow the peptide immobilization and its photorelease by recording the variation of the signal intensities of the different elements present on the SiNW surface. Mass spectrometry was performed without the use of an organic matrix and peptide ions were produced via a photocleavage mechanism. Indeed, thanks to direct photorelease achieved upon laser irradiation, a recorded predictable peak related to the molecular peptide ion has been detected, allowing the identification of the model peptide. Additional MS/MS experiments confirmed the photodissociation site and confirmed the N-terminal immobilization of the peptide on SiNWs.
authors.affiliation: Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN, UMR CNRS 8520), Avenue Poincaré, BP 60069, 59652 Villeneuve d'Ascq, France. yannick.coffinier@univ-lille1.fr.
authors.forename: Ievgen
authors.initials: I
authors.lastname: Kurylo
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, Université de Montpellier, CNRS, ENSCM, Place Eugène Bataillon, 34095 Montpellier cedex 5, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Dupré
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, Université de Montpellier, CNRS, ENSCM, Place Eugène Bataillon, 34095 Montpellier cedex 5, France.
authors.forename: Sonia
authors.identifier: http://orcid.org/0000-0002-7583-8591
authors.initials: S
authors.lastname: Cantel
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, Université de Montpellier, CNRS, ENSCM, Place Eugène Bataillon, 34095 Montpellier cedex 5, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Enjalbal
authors.affiliation: Institut de Biologie de Lille (IBL, CNRS-UMR 8161), Université de Lille Nord de France, IFR142, 1 rue du Pr. Calmette, 59021 Lille, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Drobecq
authors.affiliation: Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN, UMR CNRS 8520), Avenue Poincaré, BP 60069, 59652 Villeneuve d'Ascq, France. yannick.coffinier@univ-lille1.fr.
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
authors.affiliation: Institut de Biologie de Lille (IBL, CNRS-UMR 8161), Université de Lille Nord de France, IFR142, 1 rue du Pr. Calmette, 59021 Lille, France.
authors.forename: Oleg
authors.initials: O
authors.lastname: Melnyk
authors.affiliation: Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN, UMR CNRS 8520), Avenue Poincaré, BP 60069, 59652 Villeneuve d'Ascq, France. yannick.coffinier@univ-lille1.fr.
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.affiliation: Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN, UMR CNRS 8520), Avenue Poincaré, BP 60069, 59652 Villeneuve d'Ascq, France. yannick.coffinier@univ-lille1.fr.
authors.forename: Yannick
authors.identifier: http://orcid.org/0000-0001-7433-2102
authors.initials: Y
authors.lastname: Coffinier