title: Epitranscriptomic regulation of viral replication.
abstract: RNA plays central roles in biology and novel functions and regulation mechanisms are constantly emerging. To accomplish some of their functions within the cell, RNA molecules undergo hundreds of chemical modifications from which N6-methyladenosine (m6A), inosine (I), pseudouridine (ψ) and 5-methylcytosine (5mC) have been described in eukaryotic mRNA. Interestingly, the m6A modification was shown to be reversible, adding novel layers of regulation of gene expression through what is now recognized as epitranscriptomics. The development of molecular mapping strategies coupled to next generation sequencing allowed the identification of thousand of modified transcripts in different tissues and under different physiological conditions such as viral infections. As intracellular parasites, viruses are confronted to cellular RNA modifying enzymes and, as a consequence, viral RNA can be chemically modified at some stages of the replication cycle. This review focuses on the chemical modifications of viral RNA and the impact that these modifications have on viral gene expression and the output of infection. A special emphasis is given to m6A, which was recently shown to play important yet controversial roles in different steps of the HIV-1, HCV and ZIKV replication cycles.
authors.affiliation: Molecular and Cellular Virology Laboratory, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Independencia 834100, Santiago, Chile.
authors.forename: Camila
authors.initials: C
authors.lastname: Pereira-Montecinos
authors.affiliation: Molecular and Cellular Virology Laboratory, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Independencia 834100, Santiago, Chile.
authors.forename: Fernando
authors.initials: F
authors.lastname: Valiente-Echeverría
authors.affiliation: Molecular and Cellular Virology Laboratory, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Independencia 834100, Santiago, Chile. Electronic address: rsotorifo@med.uchile.cl.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Soto-Rifo
mesh_terms: D000818:Animals; D044127:Epigenesis, Genetic; D006801:Humans; D008957:Models, Genetic; D017393:RNA Editing / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
keywords: Epitranscriptomics; Gene expression; HCV; HIV-1; Viral replication; ZIKV; m(6)A