title: FOXF2 promoter methylation is associated with prognosis in esophageal squamous cell carcinoma.
abstract: Esophageal squamous cell carcinoma is a commonly malignant tumor of digestive tract with poor prognosis. Previous studies suggested that forkhead box F2 ( FOXF2) could be a candidate gene for assessing and predicting the prognosis of human cancers. However, the relationship between FOXF2 promoter methylation and the prognosis of esophageal squamous cell carcinoma remained unclear. Formalin-fixed, paraffin-embedded esophageal squamous cell carcinoma tissues of 135 esophageal squamous cell carcinoma patients were detected for FOXF2 promoter methylation status by methylation-specific polymerase chain reaction approach. DNA methylation results were evaluated with regard to clinicopathological features and overall survival. Our study confirmed that FOXF2 promoter hypermethylation could independently predict a poorer overall survival of esophageal squamous cell carcinoma patients ( p = 0.002), which was consistent with the data mining results of the data from 82 esophageal squamous cell carcinoma patients in The Cancer Genome Atlas datasets ( p = 0.036). In addition, no correlation was found between FOXF2 promoter methylation and other clinic pathological parameters (age, gender, differentiation, lymph node metastasis, stage, cutting edge, vascular invasion, smoking behavior, and drinking history). In conclusion, FOXF2 methylation might be a useful prognostic biomarker for esophageal squamous cell carcinoma patients.
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Chen
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Haochang
authors.initials: H
authors.lastname: Hu
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Guili
authors.initials: G
authors.lastname: Liu
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Xiuru
authors.initials: X
authors.lastname: Ying
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Ye
authors.affiliation: 2 Department of Medical Oncology, Shaoxing People's Hospital (Shaoxing Hospital of Zhejiang University), Shaoxing, China.
authors.forename: Dongping
authors.initials: D
authors.lastname: Wu
authors.affiliation: 1 Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000601:surgery; D019175:DNA Methylation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000601:surgery; D000077277:Esophageal Squamous Cell Carcinoma; D051858:Forkhead Transcription Factors / Q000235:genetics*; D005557:Formaldehyde; D006367:HeLa Cells; D056945:Hep G2 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020014:K562 Cells; D016612:Paraffin Embedding; D011237:Predictive Value of Tests; D011401:Promoter Regions, Genetic; D016019:Survival Analysis; D016707:Tissue Fixation; D016896:Treatment Outcome
chemical_list: C486413:FOXF2 protein, human; D051858:Forkhead Transcription Factors; D005557:Formaldehyde
keywords: DNA methylation; Esophageal squamous cell carcinoma; FOXF2; methylation-specific polymerase chain reaction; prognosis