title: Expression profiles of eNOS, iNOS and microRNA-27b in the corpus cavernosum of rats submitted to chronic alcoholism and Diabetes mellitus.
abstract: To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies.
  Forty eight Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D) and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study protein expressions of eNOS and iNOS by immunohistochemistry and expression of miRNA-27b in the corpus cavernosum and peripheral blood.
  Immunohistochemistry for eNOS and iNOS showed an increase in cavernosal smooth muscle cells in the alcoholic, diabetic and alcoholic-diabetic groups when compared with the control group. Similarly, the mRNA levels for eNOS were increased in cavernosal smooth muscle (CSM) in the alcoholic, diabetic and alcoholic-diabetic groups and miRNA-27b were decreased in CSM in the alcoholic, diabetic and alcoholic-diabetic groups.
  The major new finding of our study was an impairment of relaxation of cavernosal smooth muscle in alcoholic, diabetic, and alcoholic-diabetic rats that involved a decrease in the nitric oxide pathway by endothelium-dependent mechanisms accompanied by a change in the corpus cavernosum contractile sensitivity.
authors.affiliation: Master, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil. Acquisition and interpretation of data, manuscript writing.
authors.forename: Joao Paulo da
authors.initials: JP
authors.lastname: Cunha
authors.affiliation: Pos-doctoral Fellow student, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Technical procedures, manuscript writing.
authors.forename: Fermino Sanches
authors.initials: FS
authors.lastname: Lizarte
authors.affiliation: Assistant Professor, Department of Health Sciences, Universidade de Marília, Brazil. Technical procedures, manuscript writing.
authors.forename: Paulo Cezar
authors.initials: PC
authors.lastname: Novais
authors.affiliation: Fellow Master degree, Postgraduate Program in Clinical Surgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Manuscript writing.
authors.forename: Daniela
authors.initials: D
authors.lastname: Gattas
authors.affiliation: Assistant Professor, Institute of Biological and Health Sciences, Federal University of Alagoas, Alagoas, Brazil and PhD Student, Clinical Surgery Postgraduate Program, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Manuscript writing.
authors.forename: Camila Albuquerque Mello de
authors.initials: CA
authors.lastname: Carvalho
authors.affiliation: Assistant Professor, Department of Surgery and Anatomy, Surgical Clinic Program, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil, Scientific and intellectual content of the study.
authors.forename: Daniela Pretti da Cunha
authors.initials: DP
authors.lastname: Tirapelli
authors.affiliation: Assistant Professor, Division of Urology, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Scientific and intellectual content of the study.
authors.forename: Carlos Augusto Fernandes
authors.initials: CA
authors.lastname: Molina
authors.affiliation: Associate Professor, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Scientific and intellectual content of the study.
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Tirapelli
authors.affiliation: Associate Professor, Division of Urology, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Ribeirao Preto-SP, Brazil. Concept, design, intellectual and scientific content of the study; critical revision; supervised all phases of the study.
authors.forename: Silvio
authors.initials: S
authors.lastname: Tucci
mesh_terms: D000437:Alcoholism / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D052247:Nitric Oxide Synthase Type II / Q000032:analysis*; D052250:Nitric Oxide Synthase Type III / Q000032:analysis*; D010413:Penis / Q000737:chemistry* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: C526366:MIRN29 microRNA, rat; D035683:MicroRNAs; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat