title: Highly Efficient Electrochemiluminescent Silver Nanoclusters/Titanium Oxide Nanomaterials as a Signal Probe for Ferrocene-Driven Light Switch Bioanalysis.
abstract: In this work, a ferrocene (Fc)-driven light switch biosensor for ultrasensitive detection of amyloid-β (Aβ) was designed by utilizing the highly efficient electrochemiluminescent nanomaterials of silver nanoclusters/titanium oxide nanoflowers (Ag NCs/TiO2NFs) as signal labels. Through combining the TiO2NFs as the coreaction accelerator and dissolved O2as the intrinsic coreactant to in situ generate the strong oxidizing intermediate radical OH•, the electrochemiluminescence (ECL) of Ag NCs on the TiO2NFs surface could be significantly promoted in comparison with that of pure Ag NCs in solution. Further, Fc-labeled DNA as the ECL quenching probe was introduced to dramatically restrain the ECL emission of nanomaterials, which facilitated improving the sensitivity of the prepared biosensor to a large extent. Surprisingly, tiny amounts of target protein could be recognized by the immunoreaction-induced DNA nanostructure for outputting numerous secondary-target DNAs, which further triggered the release of Fc to recover the ECL signal, realizing the ultrasensitive detection of target. As a result, this developed assay for Aβ detection demonstrated excellent sensitivity with a linear range from 50 fg/mL to 500 ng/mL and limit of detection down to 32 fg/mL, which opened up a new research direction for ultrasensitive ECL bioanalysis-based metal NCs.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Ruo
authors.identifier: 0000-0003-3664-6236
authors.initials: R
authors.lastname: Yuan
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D005296:Ferrous Compounds / Q000737:chemistry*; D008027:Light; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D000075163:Metallocenes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D005296:Ferrous Compounds; D049408:Luminescent Agents; D000075163:Metallocenes; C009495:titanium dioxide; D012834:Silver; D014025:Titanium; C004998:ferrocene