title: Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three-Dimensional Fibrin Gel.
abstract: Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three-dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell-based therapies for various muscle disorders. Stem Cells Translational Medicine 2017;6:1412-1423.
authors.affiliation: Division of Hematology/Oncology, Department of Medicine and Cancer Center, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Division of Hematology/Oncology, Department of Medicine and Cancer Center, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Yalu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Division of Hematology/Oncology, Department of Medicine and Cancer Center, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Furen
authors.initials: F
authors.lastname: Wu
authors.affiliation: Division of Hematology/Oncology, Department of Medicine and Cancer Center, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Yuanfan
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois, USA.
authors.forename: Gajendra
authors.initials: G
authors.lastname: Shekhawat
authors.affiliation: Division of Hematology/Oncology, Department of Medicine and Cancer Center, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Mosenson
authors.affiliation: Division of Hematology/Oncology, Department of Medicine and Cancer Center, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Wen-Shu
authors.initials: WS
authors.lastname: Wu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D005337:Fibrin / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D005782:Gels; D005337:Fibrin
keywords: Muscle stem cells; Myogenic progenitor cells; Satellite cells; Three-dimensional fibrin gel