title: Acinetobacter halotolerans sp. nov., a novel halotolerant, alkalitolerant, and hydrocarbon degrading bacterium, isolated from soil.
abstract: A novel aerobic, non-motile, halotolerant, alkalitolerant, hydrocarbon degrading, and rod shaped bacterium, designated strain R160T, was isolated from soil in South Korea. Cells were Gram-staining-negative, catalase-positive, and oxidase-negative. This strain grew up to 7% of NaCl and in the pH range of 6-11 (optimum 7.0-10.0). The isolate degraded 51.7 ± 1.3% of hydrocarbon components (C-18, C-20, and C-22) and 45.8 ± 1.4% oil components (kerosene, diesel, and gasoline). Phylogenetic analysis based on 16 S rRNA gene sequences revealed that strain R160Tformed a lineage within the genus Acinetobacter, and was closely related to 'Acinetobacter oleivorans' DR1T(97.47%, sequence similarity). Other closely related members have sequence similarity between 97.47 to 96.52%. The predominant respiratory lipoquinones of strain R160Twere ubiquinone 9 (Q-9) and ubiquinone 8 (Q-8). The major polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylcholine (PC). The major cellular fatty acids were 9-octadecenoic acid (C18:1ω9c), hexadecanoic acid (C16:0), and summed feature (comprising C16:1ω7c and/or C16:1ω6c). The DNA G + C content of strain R160Twas 44.9 mol%. On the basis of phenotypic, genotypic, chemotaxonomic, and phylogenetic characteristics, strain R160Trepresents a novel species of the genus Acinetobacter, for which the name Acinetobacter halotolerans sp. nov. is proposed. The type strain is R160T(= KEMB 9005-333T= KACC 18453T= JCM 31009T).
authors.affiliation: Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea.
authors.forename: Ram Hari
authors.initials: RH
authors.lastname: Dahal
authors.affiliation: Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea.
authors.forename: Dhiraj Kumar
authors.initials: DK
authors.lastname: Chaudhary
authors.affiliation: Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea. jkimtamu@kgu.ac.kr.
authors.forename: Jaisoo
authors.initials: J
authors.lastname: Kim
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005742:Gasoline; D006838:Hydrocarbons / Q000378:metabolism*; D007648:Kerosene; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D005742:Gasoline; D006838:Hydrocarbons; D007648:Kerosene; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D014451:Ubiquinone; C030778:ubiquinone 8; C030571:ubiquinone 9
keywords: Acinetobacter halotolerans sp. nov.; Alkalitolerant; Halotolerant; Hydrocarbon degrading bacteria