title: Serum cytokine/chemokine profiles in patients with dengue fever (DF) and dengue hemorrhagic fever (FHD) by using protein array.
abstract: DENV infection can induce different clinical manifestations varying from mild forms to dengue fever (DF) or the severe hemorrhagic fever (DHF). Several factors are involved in the progression from DF to DHF. No marker is available to predict this progression. Such biomarker could allow a suitable medical care at the beginning of the infection, improving patient prognosis.
  
  
  
  The aim of this study was to compare the serum expression levels of acute phase proteins in a well-established cohort of dengue fever (DF) and dengue hemorrhagic fever (DHF) patients, in order to individuate a prognostic marker of diseases severity.
  
  
  
  The serum levels of 36 cytokines, chemokines and acute phase proteins were determined in DF and DHF patients and compared to healthy volunteers using a multiplex protein array and near-infrared (NIR) fluorescence detection. Serum levels of IL-1ra, IL-23, MIF, sCD40 ligand, IP-10 and GRO-α were also determined by ELISA.
  
  
  
  At the early stages of infection, GRO-α and IP-10 expression levels were different in DF compared to DHF patients. Besides, GRO-α was positively correlated with platelet counts and IP-10 was negatively correlated with total protein levels.
  
  
  
  These findings suggest that high levels of GRO-α during acute DENV infection may be associated with a good prognosis, while high levels of IP-10 may be a warning sign of infection severity.
authors.affiliation: Departamento de Virologia, Centro de Pesquisas Aggeu Magalhães-Fundação Oswaldo Cruz-Fiocruz, Recife, PE, Brazil.
authors.forename: Renato Antonio Dos Santos
authors.initials: RADS
authors.lastname: Oliveira
authors.affiliation: Departamento de Virologia, Centro de Pesquisas Aggeu Magalhães-Fundação Oswaldo Cruz-Fiocruz, Recife, PE, Brazil.
authors.forename: Marli Tenório
authors.initials: MT
authors.lastname: Cordeiro
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, PE, Brazil.
authors.forename: Patrícia Muniz Mendes Freire de
authors.initials: PMMF
authors.lastname: Moura
authors.affiliation: Faculdade de Ciências Médicas, Universidade de Pernambuco, Recife, PE, Brazil.
authors.forename: Paulo Neves Bapti
authors.initials: PNB
authors.lastname: Baptista Filho
authors.affiliation: Department of Electrical and Computer Engineering, Texas A&M University, TX, USA.
authors.forename: Ulisses de Mendonça
authors.initials: UM
authors.lastname: Braga-Neto
authors.affiliation: Departamento de Virologia, Centro de Pesquisas Aggeu Magalhães-Fundação Oswaldo Cruz-Fiocruz, Recife, PE, Brazil; Department of Infectious Diseases and Microbiology, Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Ernesto Torres de Azevedo
authors.initials: ETA
authors.lastname: Marques
authors.affiliation: Departamento de Virologia, Centro de Pesquisas Aggeu Magalhães-Fundação Oswaldo Cruz-Fiocruz, Recife, PE, Brazil. Electronic address: laura@cpqam.fiocruz.br.
authors.forename: Laura Helena Vega Gonzales
authors.initials: LHVG
authors.lastname: Gil
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D003715:Dengue / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D011379:Prognosis; D040081:Protein Array Analysis; D044967:Serum / Q000737:chemistry; D014838:Volunteers; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines
keywords: Cytokines protein array; Dengue fever; Dengue hemorrhagic fever; Prognostic marker