title: Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation.
abstract: High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data descriptor contains a confocal fluorescence, microscopic image dataset. We also describe an open source, automated image analysis workflow that can be reused to perform high-content analysis of other organelles. This dataset is suitable for analysis of morphological parameters that are linked to human umbilical vein endothelial cell (HUVEC) biology.
authors.affiliation: MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Robin
authors.initials: R
authors.lastname: Ketteler
authors.affiliation: MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Jamie
authors.initials: J
authors.lastname: Freeman
authors.affiliation: MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Francesco
authors.initials: F
authors.lastname: Ferraro
authors.affiliation: MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Nicole
authors.initials: N
authors.lastname: Bata
authors.affiliation: MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Dan F
authors.identifier: 0000-0002-4288-7530
authors.initials: DF
authors.lastname: Cutler
authors.affiliation: MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Janos
authors.identifier: 0000-0002-0149-145X
authors.initials: J
authors.lastname: Kriston-Vizi
authors.affiliation: MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Stevenson
mesh_terms: D002460:Cell Line; D018274:Electroporation; D004730:Endothelium, Vascular; D006801:Humans; D011494:Protein Kinases; D034741:RNA, Small Interfering; D020302:Weibel-Palade Bodies
chemical_list: D034741:RNA, Small Interfering; D011494:Protein Kinases