title: A fully automated flow-based approach for accelerated peptide synthesis.
abstract: Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Mijalis
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Dale A
authors.initials: DA
authors.lastname: Thomas
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Simon
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Adamo
authors.affiliation: R.M. Beaumont Corporation, Brunswick, Maine, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Beaumont
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Klavs F
authors.identifier: 0000-0001-7192-580X
authors.initials: KF
authors.lastname: Jensen
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Bradley L
authors.initials: BL
authors.lastname: Pentelute
mesh_terms: D001331:Automation / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010455:Peptides