title: The effect of lysophosphatidic acid together with interferon tau on the global transcriptomic profile in bovine endometrial cells.
abstract: In cows, lysophosphatidic acid (LPA), which acts in an auto/paracrine manner, serves as a luteotropic factor during early pregnancy by stimulating progesterone and prostaglandin E2secretion, thus protecting the bovine corpus luteum and early embryo development. Our hypothesis was that LPA exerted some local effects on the bovine endometrium prior to early embryo-maternal interactions and that interferon tau (IFNτ), the pregnancy recognition signal, modulated this action. In the present study, we applied an in vitro model involving whole-transcriptomic profiling to examine the effects of LPA on gene expression in bovine endometrial cells. Microarray analyses revealed 36, 269 and 284 differentially expressed transcripts in bovine endometrial cells in the control vs. LPA, control vs. LPA + IFNτ and LPA vs. LPA + IFNτ groups, respectively. The expression of matrix metalloproteinase 13 (MMP13) and radical S-adenosyl methionine domain containing 2 (RSAD2) was increased in the LPA-treated endometrial cells. Among the transcripts differentially regulated by LPA together with IFNτ, many of the genes were classical- or novel-type I IFN-stimulated genes (ISGs). The results indicated that 10 of the 16 analyzed genes showed a positive correlation with their corresponding microarray data upon real-time PCR validation, indicating a considerable consistency between both techniques. In summary, these transcriptional profiling studies identified a number of genes that were regulated by LPA alone and LPA together with IFNτ in endometrial cells from the bovine uterus. Available studies support the idea that LPA, which acts in an auto/paracrine manner on the endometrium, alters the expression of genes that are probably important for uterine receptivity, maternal immune tolerance to the embryo and conceptus growth and development during early pregnancy. Moreover, the differentially expressed genes (DEGs) that increased in the LPA + IFNτ-treated endometrial cells are largely in response to IFNτ actions and are possibly associated with crucial biological processes during the peri-implantation period of pregnancy.
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Boruszewska
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Kowalczyk-Zieba
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Sinderewicz
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Grycmacher
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Staszkiewicz
authors.affiliation: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland. Electronic address: i.woclawek-potocka@pan.olsztyn.pl.
authors.forename: Izabela
authors.initials: I
authors.lastname: Woclawek-Potocka
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007370:Interferon Type I / Q000008:administration & dosage / Q000494:pharmacology*; D008246:Lysophospholipids / Q000008:administration & dosage / Q000494:pharmacology*; D011257:Pregnancy Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D059467:Transcriptome / Q000502:physiology*
chemical_list: D007370:Interferon Type I; D008246:Lysophospholipids; D011257:Pregnancy Proteins; C020947:interferon tau; C032881:lysophosphatidic acid
keywords: Cow; Endometrial cells; Interferon tau; Lysophosphatidic acid; Microarray