title: Oral administration of fumonisin B1and T-2 individually and in combination affects hepatic total and mitochondrial membrane lipid profile of rabbits.
abstract: Weaned rabbits were fed diets contaminated with 2 mg/kg diet T-2 toxin alone, or 10 mg/kg diet fumonisin B1(FB1) alone, and both toxins in combination (2 + 10 mg/kg, respectively) compared to a toxin-free control diet. Samplings were performed after 4 weeks (blood and liver). Bodyweight of T-2-fed group was lower after 4 weeks; the liver weight was increased dramatically (threefold of control). Liver total phospholipids (PLs) provided slight alterations in the fatty acid (FA) composition; all three toxin-treated groups showed a decrease in palmitoleic acid (C16:1 n7) proportion. In the liver mitochondrial PL FA composition, margaric acid (C17:0) proportion decreased in the separated toxin treatments compared to the combined setting. Oleic acid (C18:1 n9) proportion was increased and arachidonic acid (C20:4 n6) was decreased in the FB1-treated group, while docosapentaenoic acid (C22:5 n3) was decreased in the separated treatments. The total monounsaturation was significantly higher in the FB1group's mitochondrial PL FA profile. After 4 weeks, all toxin treatments decreased the blood plasma reduced glutathione and glutathione peroxidase activity, and FB1increased the plasma sphinganine/sphingosine ratio. Both mycotoxins seem to cross the hepatocellular and the hepatic mitochondrial membrane, without drastic membrane disruption, as assessed from the PL FA composition, but inducing detectable lipid peroxidation.
authors.affiliation: 1 Institute of Diagnostic Imaging and Radiation Oncology, Kaposvár University , Kaposvár, Hungary.|2 "MTA-KE Mycotoxins in the Food Chain" Research Group, Hungarian Academy of Sciences, Kaposvár University , Kaposvár, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Szabó
authors.affiliation: 2 "MTA-KE Mycotoxins in the Food Chain" Research Group, Hungarian Academy of Sciences, Kaposvár University , Kaposvár, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Szabó-Fodor
authors.affiliation: 3 Research Institute for Animal Breeding, Nutrition and Meat Science, National Agricultural Research Center , Herceghalom, Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Fébel
authors.affiliation: 4 Department of Nutrition, Faculty of Agricultural and Environmental Sciences, Szent István University , Gödöllő, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Mézes
authors.affiliation: 1 Institute of Diagnostic Imaging and Radiation Oncology, Kaposvár University , Kaposvár, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Bajzik
authors.affiliation: 2 "MTA-KE Mycotoxins in the Food Chain" Research Group, Hungarian Academy of Sciences, Kaposvár University , Kaposvár, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Kovács
mesh_terms: D000284:Administration, Oral; D000818:Animals; D037341:Fumonisins / Q000008:administration & dosage*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D013605:T-2 Toxin / Q000008:administration & dosage*
chemical_list: D037341:Fumonisins; D008563:Membrane Lipids; C056933:fumonisin B1; D013605:T-2 Toxin
keywords: FB1; T-2; lipid peroxidation; liver; membrane lipids; mitochondria; rabbit