title: Transplantation of Skeletal Muscle Stem Cells.
abstract: Transplanting adult stem cells provides a stringent test for self-renewal and the assessment of comparative engraftment in competitive transplant assays. Transplantation of satellite cells into mammalian skeletal muscle provided the first critical evidence that satellite cells function as adult muscle stem cells. Transplantation of a single satellite cell confirmed and extended this hypothesis, providing proof that the satellite cell is a bona fide adult skeletal muscle stem cell as reported by Sacco et al. (Nature 456(7221):502-506). Satellite cell transplantation has been further leveraged to identify culture conditions that maintain engraftment and to identify self-renewal deficits in satellite cells from aged mice. Conversion of iPSCs (induced pluripotent stem cells) to a satellite cell-like state, followed by transplantation, demonstrated that these cells possess adult muscle stem cell properties as reported by Darabi et al. (Stem Cell Rev Rep 7(4):948-957) and Mizuno et al. (FASEB J 24(7):2245-2253). Thus, transplantation strategies involving either satellite cells derived from adult muscles or derived from iPSCs may eventually be exploited as a therapy for treating patients with diseased or failing skeletal muscle. Here, we describe methods for isolating dispersed adult mouse satellite cells and satellite cells on intact myofibers for transplantation into recipient mice to study muscle stem cell function and behavior following engraftment .
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA.
authors.forename: Monica N
authors.initials: MN
authors.lastname: Hall
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA.
authors.forename: John K
authors.initials: JK
authors.lastname: Hall
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA.
authors.forename: Adam B
authors.initials: AB
authors.lastname: Cadwallader
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA.
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Pawlikowski
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Doles
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA.
authors.forename: Tiffany L
authors.initials: TL
authors.lastname: Elston
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA. Olwin@colorado.edu.
authors.forename: Bradley B
authors.initials: BB
authors.lastname: Olwin
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D015415:Biomarkers; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000637:transplantation; D018482:Muscle, Skeletal / Q000166:cytology*; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Adult muscle stem cell; Muscle myofiber; Satellite cell; Skeletal muscle; Stem cell transplantation