title: Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry.
abstract: Global obesity epidemic demands more effective therapeutic treatments and better understanding of obesity pathophysiology. Since obesity results from energy imbalance, accurate quantification of energy intake and energy expenditure (EE) becomes an essential prerequisite to phenotype the cause for obesity development. Indirect calorimetry has long been used as one of the most established methods in EE quantification by detecting changes in levels of O2consumption and CO2production. In this article, we describe procedures and important considerations for an effective measurement using indirect calorimetry.
authors.affiliation: Brown Foundation Institute of Molecular Medicine, University of Texas McGovern Medical School, 1825 Pressler St., SRB 430G, Houston, TX, 77030, USA.
authors.forename: Eun Ran
authors.initials: ER
authors.lastname: Kim
authors.affiliation: Brown Foundation Institute of Molecular Medicine, University of Texas McGovern Medical School, 1825 Pressler St., SRB 430G, Houston, TX, 77030, USA. Qingchun.tong@uth.tmc.edu.|Department of Neurobiology and Anatomy, University of Texas McGovern Medical School, Houston, TX, 77030, USA. Qingchun.tong@uth.tmc.edu.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Tong
mesh_terms: D000818:Animals; D002153:Calorimetry, Indirect / Q000379:methods; D004734:Energy Metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000378:metabolism; D010101:Oxygen Consumption; D013223:Statistics as Topic / Q000379:methods
keywords: Energy expenditure; Indirect calorimetry; Metabolic rate; Obesity; Oxygen consumption