title: Comparison between in vitro properties of washed platelet concentrates suspended in M-sol and those in BRS-A, both of which were prepared with an automated cell processor.
abstract: BACKGROUND
  Washed platelet concentrate (WPC) is prepared manually in general, but automated preparation is desirable to minimize variation in the WPC quality and enhance WPC production. Recently, the software was improved for an automated cell processor (ACP) to control all processes of WPC preparation. M-sol and BRS-A, which are mixtures of medical solutions, are widely used for WPC preparation with a manual method in Japan. In this study, we prepared WPC suspended in M-sol (WPC-M) or BRS-A (WPC-B) with the ACP, and compared their in vitro properties during 7-day storage.
  
  
  METHODS
  PC was divided into two equal aliquots for WPC-M and WPC-B. A divided PC, medical solutions and disposable materials were set in the ACP, and it was started to prepare WPC-M or WPC-B on Day 0. Prepared WPC was stored on a flatbed shaker until Day 7.
  
  
  RESULTS
  The pH of WPC-M and WPC-B was maintained above 6.8 during the 7-day storage. The differences in aggregation (%), HSR (%), P-selectin expression, GPIbα expression, and phosphatidylserine expression between WPC-M and WPC-B were minimal until Day 3.
  
  
  CONCLUSIONS
  The in vitro properties of WPC-B are not markedly different from those of WPC-M until Day 3.
authors.affiliation: Central Blood Institute, Japanese Red Cross Society, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Iwama
authors.affiliation: Central Blood Institute, Japanese Red Cross Society, Tokyo, Japan. Electronic address: j-hirayama@jrc.or.jp.
authors.forename: Junichi
authors.initials: J
authors.lastname: Hirayama
authors.affiliation: Central Blood Institute, Japanese Red Cross Society, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nogawa
authors.affiliation: Central Blood Institute, Japanese Red Cross Society, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shiba
authors.affiliation: Central Blood Institute, Japanese Red Cross Society, Tokyo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Satake
authors.affiliation: Japanese Red Cross Hokkaido Block Blood Center, Sapporo, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Takamoto
authors.affiliation: Central Blood Institute, Japanese Red Cross Society, Tokyo, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Tadokoro
mesh_terms: D001331:Automation; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D019999:Pharmaceutical Solutions; D010983:Plateletpheresis
chemical_list: D019999:Pharmaceutical Solutions
keywords: Additive solution; Automated cell processor; BRS-A; M-sol; Platelet storage; Platelet washing