title: [Sperm DNA fragmentation: association with semen parameters in young men].
abstract: OBJECTIVE
  Abnormal sperm DNA integrity is an important risk factor for male infertility. The aim of this work was to examine sperm DNA fragmentation in a cohort of young male volunteers (n=111; age 21.0+/-0.2 years) from the general population and establish the association between the level of sperm DNA fragmentation and sperm functional parameters.
  
  
  METHODS
  Sperm DNA fragmentation index (DFI) was determined by SCSA (sperm chromatin structure assay) using flow cytometry. Standard semen parameters (concentration, motility, and morphology) were evaluated according to the WHO guidelines (2010).
  
  
  RESULTS
  and conclusions. In the study cohort, 79.0%, 12.4% and 8.6% of men had normal (DFI<15%), borderline (15 less or equal DFI<27%) and high (DFI more or equal 27%) levels of fragmentation, respectively. Men with impaired spermatogenesis had greater IDF values (14.53+/-1.43%) than men with normal semen parameters (8.88+/-0.77%, p<0.05). There was a statistically significant negative correlation between IFD and ejaculate concentration (r=-0.21, p<0.05), fractions of mobile (r=-0.41, p<0.05) and morphologically normal sperm (r=- 0.34, p<0.05). Testing sperm DNA fragmentation using SCSA technique can be employed in epidemiological studies of male fertility.
authors.affiliation: Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS, Novosibirsk.
authors.forename: L V
authors.initials: LV
authors.lastname: Osadchuk
authors.affiliation: Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS, Novosibirsk.|Krasnoyarsk Center for Reproductive Medicine, Krasnoyarsk.
authors.forename: D A
authors.initials: DA
authors.lastname: Tataru
authors.affiliation: Ergin Medical center, Kemerovo.
authors.forename: N N
authors.initials: NN
authors.lastname: Kuznetsova
authors.affiliation: Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS, Novosibirsk.
authors.forename: M A
authors.initials: MA
authors.lastname: Kleshev
authors.affiliation: Krasnoyarsk Center for Reproductive Medicine, Krasnoyarsk.
authors.forename: E V
authors.initials: EV
authors.lastname: Markova
authors.affiliation: Krasnoyarsk Center for Reproductive Medicine, Krasnoyarsk.
authors.forename: A V
authors.initials: AV
authors.lastname: Svetlakov
mesh_terms: D000293:Adolescent; D000328:Adult; D002843:Chromatin / Q000737:chemistry; D053938:DNA Fragmentation; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis*; D008297:Male; D012661:Semen / Q000737:chemistry*; D013076:Sperm Count; D013091:Spermatogenesis; D013094:Spermatozoa / Q000648:ultrastructure*; D055815:Young Adult
chemical_list: D002843:Chromatin
keywords: concentration; morphology; motility; sperm DNA fragmentation