title: Fluoride Alteration of [3H]Glucose Uptake in Wistar Rat Brain and Peripheral Tissues.
abstract: The present study was designed to investigate the role of postnatal fluoride intake on [3H]glucose uptake and transport in rat brain and peripheral tissues. Sodium fluoride (NaF) in a concentration of 10 or 50 ppm was added to the drinking water of adult Wistar rats. The control group received distilled water. After 4 weeks, respective plasma fluoride levels were 0.0541 ± 0.0135 μg/ml (control), 0.0596 ± 0.0202 μg/ml (10 ppm), and 0.0823 ± 0.0199 μg/ml (50 ppm). Although plasma glucose levels were not altered in any group, the plasma insulin level in the fluoride (50 ppm) group was elevated (0.72 ± 0.13 μg/ml) versus the control group (0.48 ± 0.24 μg/ml) and fluoride (10 ppm) group. In rats receiving fluoride for 4 weeks at 10 ppm in drinking water, [3H]glucose uptake was unaltered in all tested parts of the brain. However, in rats receiving fluoride at 50 ppm, [3H]glucose uptake in cerebral cortex, hippocampus, and thalamus with hypothalamus was elevated, versus the saline group. Fluoride intake had a negligible effect on [3H]glucose uptake by peripheral tissues (liver, pancreas, stomach, small intestine, atrium, aorta, kidney, visceral tissue, lung, skin, oral mucosa, tongue, salivary gland, incisor, molars, and jawbone). In neither fluoride group was glucose transporter proteins 1 (GLUT 1) or 3 (GLUT 3) altered in frontal cortex and striatum versus control. On the assumption that increased glucose uptake (by neural tissue) reasonably reflects neuronal activity, it appears that fluoride damage to the brain results in a compensatory increase in glucose uptake and utilization without changes in GLUT 1 and GLUT 3 expression.
authors.affiliation: Department of Toxicology and Health Protection, School of Public Health in Bytom, Medical University of Silesia, Piekarska 18, 41-902, Bytom, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Rogalska
authors.affiliation: Department of Neuro-Psychopharmacology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343, Kraków, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kuter
authors.affiliation: Department of Toxicology and Health Protection, School of Public Health in Bytom, Medical University of Silesia, Piekarska 18, 41-902, Bytom, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Żelazko
authors.affiliation: Department of Communal Hygiene and Sanitary Supervision, School of Public Health in Bytom, Medical University of Silesia, Piekarska 18, 41-902, Bytom, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Głogowska-Gruszka
authors.affiliation: Department of Biochemistry, Clinical Biochemistry Division, Medical University of Silesia, Jordana 19, 41-808, Zabrze, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Świętochowska
authors.affiliation: Department of Toxicology and Health Protection, School of Public Health in Bytom, Medical University of Silesia, Piekarska 18, 41-902, Bytom, Poland. pnowak@sum.edu.pl.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Nowak
mesh_terms: D000818:Animals; D001786:Blood Glucose; D001921:Brain / Q000378:metabolism*; D005459:Fluorides / Q000097:blood; D005947:Glucose / Q000493:pharmacokinetics*; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D051274:Glucose Transporter Type 3 / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008297:Male; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology*; D014018:Tissue Distribution; D014316:Tritium / Q000493:pharmacokinetics*
chemical_list: D001786:Blood Glucose; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D007328:Insulin; C495679:Slc2a1 protein, rat; C495690:Slc2a3 protein, rat; D014316:Tritium; D012969:Sodium Fluoride; D005947:Glucose; D005459:Fluorides
keywords: Brain; Exposure; Fluoride; Glucose transporter; Glucose uptake; Rats