title: Molecular assessment of the bacterial community associated with Cassava (Manihot esculenta Crantz) cultivation in Cameroon.
abstract: Bacterial communities play an important role in nutrient cycles and plant development. Their distribution and activity may depend on location and environmental heterogeneity. This study characterized soil bacterial communities in cassava fields of Eastern (Andom) and Southern (Bityili) Cameroon using molecular tools. In both sites, two improved varieties (TMS-96/1414; TMS-92/0326) and a local variety (Local) were grown in a randomized block design. Composite bulk soils were collected at 10months after planting from cassava plots. The 16S rDNA region was amplified, MiSeq was performed and sequence data analyzed. The same 17 bacterial phyla were present in both Andom and Bityili, while Chlorobi and Deinococcus-Thermus were only specific to Andom. The phyla Proteobacteria, Planctomycetes, Actinobacteria and Acidobacteria were dominant. Although both sites shared similar phyla, the principal coordinate analysis revealed significant variations in their composition, suggesting that the functions of the bacteria in nutrients cycling are likely to differ between Andom and Bityili. Cassava yields were generally higher in Andom which also displayed a higher diversity of bacterial communities. This study provides useful information on the composition of bacterial communities in cassava fields in two agro-ecologies of Cameroon. It constitutes to our knowledge the first report describing soil bacterial communities in association with cassava growth in the country, using molecular tools.
authors.affiliation: Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011, Japan. Electronic address: pesseus77@yahoo.fr.
authors.forename: Papa Saliou
authors.initials: PS
authors.lastname: Sarr
authors.affiliation: Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Sugiyama
authors.affiliation: Regional Biocontrol and Applied Microbiology Laboratory, Institute for Agricultural Research and Development, B.P. 2067, Yaoundé, Cameroon.
authors.forename: Aime Didier Boyogueno
authors.initials: AD
authors.lastname: Begoude
authors.affiliation: Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011, Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Yazaki
authors.affiliation: Graduate School of Asian and African Area Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida Sakyo-ku, Kyoto, 606-8581, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Araki
authors.affiliation: Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Nawata
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D044822:Biodiversity; D002163:Cameroon; D004269:DNA, Bacterial / Q000302:isolation & purification; D004463:Ecology; D002365:Manihot / Q000254:growth & development / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: 16S rRNA; Bacterial community; Cameroon; Cassava; Molecular assessment