title: VEGF-A, sVEGFR-1, and sVEGFR-2 in BCR-ABL negative myeloproliferative neoplasms.
abstract: OBJECTIVE
  Data from the literature indicate the relationship between the bone marrow microvessel density and the blood parameters of angiogenesis. The aim of this study was to evaluate selected parameters of angiogenesis (VEGF-A, sVEGFR-1, and sVEGFR-2) and their correlations with white blood cells, platelets, and red blood cells.
  
  
  METHODS
  The study included 72 patients (mean age, 61.84 years) with myeloproliferative neoplasms (MPNs): essential thrombocythemia (ET) (n=46), polycythemia vera (PV) (n=19), and primary myelofibrosis (PMF) (n=7). Serum VEGF-A, sVEGFR-1, and sVEGFR-2 were determined using the ELISA assay.
  
  
  RESULTS
  We observed a significantly higher level of VEGF-A and reduced concentrations of sVEGFR-1 and sVEGFR-2 in the whole group of patients with MPNs as compared to controls. Detailed analysis confirmed significantly higher level of VEGF-A and lower concentration of sVEGFR-2 in each subgroups of MPNs patients. However, sVEGFR-1 concentrations were significantly lower only in PV and ET patients.
  
  
  CONCLUSIONS
  The study showed an increased level of VEGF-A, which may indicate the intensity of neoangiogenesis in the bone marrow. Decreased sVEGFR-1 and sVEGFR-2 in the blood of patients with MPNs may reflect consumption of these soluble receptors.
authors.affiliation: Department of Hematology and Malignant Diseases of Hematopoietic System, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Gadomska
authors.affiliation: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland. Electronic address: stankowska_katarzyna@wp.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Stankowska
authors.affiliation: Department of Neurological and Neurosurgical Nursing, Faculty of Health Sciences, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Boinska
authors.affiliation: Department of Neurological and Neurosurgical Nursing, Faculty of Health Sciences, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Ślusarz
authors.affiliation: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Tylicka
authors.affiliation: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Michalska
authors.affiliation: Department of Hematology and Malignant Diseases of Hematopoietic System, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jachalska
authors.affiliation: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Rość
mesh_terms: D000368:Aged; D001772:Blood Cell Count; D001853:Bone Marrow / Q000098:blood supply / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000097:blood*; D011087:Polycythemia Vera / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology*; D055728:Primary Myelofibrosis / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology*; D018709:Statistics, Nonparametric; D013920:Thrombocythemia, Essential / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000097:blood*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D005340:Fibrinogen; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2; D016044:Fusion Proteins, bcr-abl
keywords: BCR-ABL-negative myeloproliferative neoplasm; VEGF-A; sVEGFR-1; sVEGFR-2