title: Chondrocyte secretome: a source of novel insights and exploratory biomarkers of osteoarthritis.
abstract: The extracellular matrix (ECM) of articular cartilage is comprised of complex networks of proteins and glycoproteins, all of which are expressed by its resident cell, the chondrocyte. Cartilage is a unique tissue given its complexity and ability to resist repeated load and deformation. The mechanisms by which articular cartilage maintains its integrity throughout our lifetime is not fully understood, however there are numerous regulatory pathways known to govern ECM turnover in response to mechanical stimuli. To further our understanding of this field, we envision that proteomic analysis of the secretome will provide information on how the chondrocyte remodels the surrounding ECM in response to load, in addition to providing information on the metabolic state of the cell. In this review, we attempt to summarize the recent mass spectrometry-based proteomic discoveries in healthy and diseased cartilage and chondrocytes, to facilitate the discovery of novel biomarkers linked to degenerative pathologies, such as osteoarthritis (OA).
authors.affiliation: Bone and Cartilage Research Unit, Arthropôle Liège, University of Liège, CHU Sart-Tilman, Belgium; The D-BOARD European Consortium for Biomarker Discovery. Electronic address: christelle.sanchez@ulg.ac.be.
authors.forename: C
authors.initials: C
authors.lastname: Sanchez
authors.affiliation: The D-BOARD European Consortium for Biomarker Discovery; Department of Rheumatology, Biomarkers and Research, Nordic Bioscience, Herlev Hovedgade 207, 2730, Herlev, Denmark. Electronic address: acbj@Nordicbioscience.com.
authors.forename: A-C
authors.initials: AC
authors.lastname: Bay-Jensen
authors.affiliation: The D-BOARD European Consortium for Biomarker Discovery; Institute of Experimental Musculoskeletal Medicine, University Hospital Munster, Domagkstrasse 3, D-48149, Munster, Germany. Electronic address: thomas.pap@uni-muenster.de.
authors.forename: T
authors.initials: T
authors.lastname: Pap
authors.affiliation: The D-BOARD European Consortium for Biomarker Discovery; Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem, P.O. Box 12272, Jerusalem, 91120, Israel. Electronic address: monad@ekmd.huji.ac.il.
authors.forename: M
authors.initials: M
authors.lastname: Dvir-Ginzberg
authors.affiliation: The D-BOARD European Consortium for Biomarker Discovery; Department of Veterinary Pre-Clinical Sciences, School of Veterinary Medicine, University of Surrey, Guildford, GU2 7AL, United Kingdom. Electronic address: h.quasnichka@surrey.ac.uk.
authors.forename: H
authors.initials: H
authors.lastname: Quasnichka
authors.affiliation: The D-BOARD European Consortium for Biomarker Discovery; Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, Faculty of Health & Life Sciences, University of Liverpool, Liverpool, United Kingdom. Electronic address: rbj@liverpool.ac.uk.
authors.forename: R
authors.initials: R
authors.lastname: Barrett-Jolley
authors.affiliation: The D-BOARD European Consortium for Biomarker Discovery; Department of Veterinary Pre-Clinical Sciences, School of Veterinary Medicine, University of Surrey, Guildford, GU2 7AL, United Kingdom; Faculty of Health and Medical Sciences, Duke of Kent Building, University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom; Arthritis Research UK Centre for Sport, Exercise and Osteoarthritis, Queen's Medical Centre, Nottingham, NG7 2UH, United Kingdom; Center of Excellence in Genomic Medicine Research (CEGMR), King Fahd Medical Research Center (KFMRC), Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia. Electronic address: a.mobasheri@surrey.ac.uk.
authors.forename: A
authors.initials: A
authors.lastname: Mobasheri
authors.affiliation: Bone and Cartilage Research Unit, Arthropôle Liège, University of Liège, CHU Sart-Tilman, Belgium; The D-BOARD European Consortium for Biomarker Discovery. Electronic address: yhenrotin@ulg.ac.be.
authors.forename: Y
authors.initials: Y
authors.lastname: Henrotin
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002358:Cartilage, Articular / Q000378:metabolism; D019902:Chondrocytes / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D010003:Osteoarthritis / Q000175:diagnosis*; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D015415:Biomarkers; D016207:Cytokines; D004798:Enzymes; D011506:Proteins
keywords: Biomarker; Cartilage; Chondrocyte; Osteoarthritis; Proteomics; Secretome