title: Glyphosate Can Decrease Germination of Glyphosate-Resistant Soybeans.
abstract: We investigated the effects of different concentrations of glyphosate acid and one of its formulations (Roundup) on seed germination of two glyphosate-resistant (GR) and one non-GR variety of soybean. As expected, the herbicide affected the shikimate pathway in non-GR seeds but not in GR seeds. We observed that glyphosate can disturb the mitochondrial electron transport chain, leading to H2O2accumulation in soybean seeds, which was, in turn, related to lower seed germination. In addition, GR seeds showed increased activity of antioxidant systems when compared to non-GR seeds, making them less vulnerable to oxidative stress induced by glyphosate. The differences in the responses of GR varieties to glyphosate exposure corresponded to their differences in enzymatic activity related to H2O2scavenging and mitochondrial complex III (the proposed site of ROS induction by glyphosate). Our results showed that glyphosate ought to be used carefully as a pre-emergence herbicide in soybean field crop systems because this practice may reduce seed germination.
authors.affiliation: Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Avenida Antônio Carlos 6627, Pampulha, Caixa Postal 486, 31270-970 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Marcelo Pedrosa
authors.identifier: http://orcid.org/0000-0001-9406-9815
authors.initials: MP
authors.lastname: Gomes
authors.affiliation: Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Avenida Antônio Carlos 6627, Pampulha, Caixa Postal 486, 31270-970 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Elisa Monteze
authors.initials: EM
authors.lastname: Bicalho
authors.affiliation: Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Avenida Antônio Carlos 6627, Pampulha, Caixa Postal 486, 31270-970 Belo Horizonte, Minas Gerais, Brazil.|GEOTOP & Institut des Sciences de l'environnement, Université du Québec à Montréal , C.P. 8888, Succ. Centre-Ville, H3C 3P8 Montréal, Québec, Canada.
authors.forename: Élise
authors.initials: É
authors.lastname: Smedbol
authors.affiliation: Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Avenida Antônio Carlos 6627, Pampulha, Caixa Postal 486, 31270-970 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Fernanda Vieira da Silva
authors.initials: FV
authors.lastname: Cruz
authors.affiliation: GEOTOP & Institut des Sciences de l'environnement, Université du Québec à Montréal , C.P. 8888, Succ. Centre-Ville, H3C 3P8 Montréal, Québec, Canada.
authors.forename: Marc
authors.initials: M
authors.lastname: Lucotte
authors.affiliation: Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Avenida Antônio Carlos 6627, Pampulha, Caixa Postal 486, 31270-970 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Queila Souza
authors.initials: QS
authors.lastname: Garcia
mesh_terms: D018525:Germination / Q000187:drug effects*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D053209:Herbicide Resistance; D006540:Herbicides / Q000494:pharmacology*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D013025:Glycine max / Q000187:drug effects* / Q000254:growth & development; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate
keywords: Roundup; antioxidant; mitochondria; pesticide; reactive oxygen species; shikimate