title: Antibacterial Activity of Fructus forsythia Essential Oil and the Application of EO-Loaded Nanoparticles to Food-Borne Pathogens.
abstract: Fructus forsythiaessential oil (FEO) with excellent antibacterial activity was rarely reported. The objective of the present study was to investigate the antibacterial activity and the antibacterial mechanism of FEO against two food-borne pathogenic bacteria,Escherichia coli(E. coli) andStaphylococcus aureus(S. aureus) in vitro. When treated FEO, the zones of inhibition (ZOI) ofE. coli(20.5 ± 0.25 mm) andS. aureus(24.3 ± 0.21 mm) were much larger than control (p< 0.05). The minimum inhibitory concentrations (MICs) of FEO were 3.13 mg/mL and 1.56 mg/mL forE. coliandS. aureus, respectively. The antibacterial mechanism of FEO againstE. coilwas due to the changes in permeability and integrity of cell membrane leading to the leakage of nucleic acids and proteins. With the superior antibacterial activity of FEO, the nano-encapsulation method has been applied in FEO. When compared to FEO and blank chitosan nanoparticles, FEO-loaded nanoparticles (chitosan to FEO of 1:1) can effectively inhibit the growth ofE. coilabove 90% at room temperature. It is necessary to consider that FEO and FEO-loaded nanoparticles will become promising antibacterial additives for food preservative, cosmetic, and pharmaceutical applications.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. guona_910817@163.com.|Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. guona_910817@163.com.|Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, 150040 Harbin, China. guona_910817@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. gqynefu@163.com.|Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. gqynefu@163.com.|Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, 150040 Harbin, China. gqynefu@163.com.
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Gai
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. jjnefu@163.com.|Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. jjnefu@163.com.|Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, 150040 Harbin, China. jjnefu@163.com.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. wwnefu@126.com.|Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. wwnefu@126.com.|Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, 150040 Harbin, China. wwnefu@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. zyg_orl@126.com.|Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. zyg_orl@126.com.|Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, 150040 Harbin, China. zyg_orl@126.com.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. octo_9010@163.com.|Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, China. octo_9010@163.com.|Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, 150040 Harbin, China. octo_9010@163.com.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
keywords: Forsythia suspense; Fructus forsythia essential oil; antibacterial activity; antibacterial mechanism; chitosan nanoparticles