title: Serum Proteome Alterations in Patients with Cognitive Impairment after Traumatic Brain Injury Revealed by iTRAQ-Based Quantitative Proteomics.
abstract: Background. Cognitive impairment is the leading cause of traumatic brain injury- (TBI-) related disability; however, the underlying pathogenesis of this dysfunction is not completely understood.Methods. Using an isobaric tagging for relative and absolute quantitation- (iTRAQ-) based quantitative proteomic approach, serum samples from healthy control subjects, TBI patients with cognitive impairment, and TBI patients without cognitive impairment were analysed to identify differentially expressed proteins (DEPs) related to post-TBI cognitive impairment. In addition, DEPs were further analysed using bioinformatic platforms and validated using enzyme-linked immunosorbent assays (ELISA).Results. A total of 56 DEPs were identified that were specifically related to TBI-induced cognitive impairment. Bioinformatic analysis revealed that a wide variety of cellular and metabolic processes and some signaling pathways were involved in the pathophysiology of cognitive deficits following TBI. Five randomly selected DEPs were validated using ELISA in an additional 105 cases, and the results also supported the experimental findings.Conclusions. Despite limitations, our findings will facilitate further studies of the pathological mechanisms underlying TBI-induced cognitive impairment and provide new methods for the research and development of neuroprotective agents. However, further investigation on a large cohort is warranted.
authors.affiliation: Institute of Integrated Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Xin-Gui
authors.identifier: 0000-0002-8782-6563
authors.initials: XG
authors.lastname: Xiong
authors.affiliation: Institute of Integrated Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Institute of Integrated Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Chunhu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Integrated Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Yang
authors.identifier: 0000-0002-1225-6017
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Integrated Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Integrated Traditional Chinese & Western Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Weijun
authors.identifier: 0000-0002-4506-0942
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Integrated Traditional Chinese & Western Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Zhe
authors.identifier: 0000-0002-1118-9721
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Integrated Traditional Chinese & Western Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Zi-An
authors.identifier: 0000-0002-0794-2960
authors.initials: ZA
authors.lastname: Xia
mesh_terms: D000328:Adult; D000070642:Brain Injuries, Traumatic / Q000097:blood* / Q000150:complications*; D003072:Cognition Disorders / Q000097:blood* / Q000209:etiology*; D003710:Demography; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D063990:Gene Ontology; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D025941:Protein Interaction Mapping; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D015398:Signal Transduction
chemical_list: D020543:Proteome