title: [Myeloid and erythroid hematopoietic transcription factor expression decline after knockdown oflmnagenes in zebrafish embryos].
abstract: Objective:To investigate the effect oflmnagene down-regulation on early hematopoietic development of zebrafish.Methods:Phosphorodiamidate morpholino oligomer (PMO) technology was used to downregulatelmnagene expression in Zebrafish. Zebrafish embryos injected phosphorodiamidate morpholino antisense oligonucleotide oflmnagene mRNA by microinjection at unicellular stage were taken as the experimental group, and those injected meaningless phosphorodiamidate morpholino antisense oligonucleotide were taken as the control. The embryos were collected at 18, 24, 30 and 36 hpf after the fertilization. The real-time fluorescent quantitative PCR (RT-PCR) and whole embryoin situhybridization methods were used to detect the expression of myeloid hematopoietic transcription factorpu. 1and erythroid hematopoietic transcription factorgata1in zebrafish.Results:RT-PCR showed that the expressions ofpu. 1andgata1decreased in the experimental group compared with the control group (allP<0.05). Whole embryoin situhybridization showed that the blue-black positive hybridization signals ofpu. 1andgata1in experimental group were shallow than those in the control group.Conclusion:Myeloid hematopoietic and erythroid hematopoietic of zebrafish are blocked with the downregulation oflmnagene.
authors.affiliation: Department of Immunology, Guizhou Medical University, Guiyang 550004, China; Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Shifang
authors.initials: S
authors.lastname: Hou
authors.affiliation: Department of Immunology, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Clinical Medical Research Center, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang 550004, China; Children Medical Center, Guizhou Medical University, Guiyang 550004, China. hzx@gmc.edu.cn.
authors.forename: Zhixu
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Immunology, Guizhou Medical University, Guiyang 550004, China; Tissue Engineering and Stem Cell Research Center, Guizhou Medical University, Guiyang 550004, China; Laboratory Animal Center, Guizhou Medical University, Guiyang 550004, China. gyslp456@gmc.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Shu
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000503:physiopathology; D050982:GATA1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006410:Hematopoiesis; D017403:In Situ Hybridization; D034904:Lamin Type A / Q000235:genetics / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D050982:GATA1 Transcription Factor; D034904:Lamin Type A; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C440308:lamin C