title: Omics Approaches for the Study of Adaptive Immunity toStaphylococcus aureusand the Selection of Vaccine Candidates.
abstract: Staphylococcus aureusis a dangerous pathogen both in hospitals and in the community. Due to the crisis of antibiotic resistance, there is an urgent need for new strategies to combatS. aureusinfections, such as vaccination. Increasing our knowledge about the mechanisms of protection will be key for the successful prevention or treatment ofS. aureusinvasion. Omics technologies generate a comprehensive picture of the physiological and pathophysiological processes within cells, tissues, organs, organisms and even populations. This review provides an overview of the contribution of genomics, transcriptomics, proteomics, metabolomics and immunoproteomics to the current understanding ofS. aureus‑host interaction, with a focus on the adaptive immune response to the microorganism. While antibody responses during colonization and infection have been analyzed in detail using immunoproteomics, the full potential of omics technologies has not been tapped yet in terms of T-cells. Omics technologies promise to speed up vaccine development by enabling reverse vaccinology approaches. In consequence, omics technologies are powerful tools for deepening our understanding of the "superbug"S. aureusand for improving its control.
authors.affiliation: Department of Immunology, University Medicine Greifswald, Greifswald 17475, Germany. silva.holtfreter@uni-greifswald.de.
authors.forename: Silva
authors.initials: S
authors.lastname: Holtfreter
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht 3584, The Netherlands. J.B.Kolata@umcutrecht.nl.
authors.forename: Julia
authors.initials: J
authors.lastname: Kolata
authors.affiliation: Department of Immunology, University Medicine Greifswald, Greifswald 17475, Germany. sebastian.stentzel@uni-greifswald.de.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Stentzel
authors.affiliation: Department of Immunology, University Medicine Greifswald, Greifswald 17475, Germany. stephaniebauerfeind@web.de.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Bauerfeind
authors.affiliation: ZIK-FunGene Junior Research Group Applied Proteomics, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, EMA-University of Greifswald, Greifswald 17475, Germany. frank.schmidt@uni-Greifswald.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Schmidt
authors.affiliation: ZIK-FunGene Junior Research Group Applied Proteomics, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, EMA-University of Greifswald, Greifswald 17475, Germany. sundaramon@uni-greifswald.de.
authors.forename: Nandakumar
authors.initials: N
authors.lastname: Sundaramoorthy
authors.affiliation: Department of Immunology, University Medicine Greifswald, Greifswald 17475, Germany. broeker@uni-greifswald.de.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Bröker
keywords: Staphylococcus aureus; adaptive immunity; genomics; immune response; immunoproteomics; proteomics; transcriptomics; vaccine