title: Artificially designed recombinant protein composed of multiple epitopes of foot-and-mouth disease virus as a vaccine candidate.
abstract: BACKGROUND
  Concerns regarding the safety of inactivated foot-and-mouth disease (FMD) vaccine have been raised since it is produced from cultured live FMD virus (FMDV). To overcome this issue, recombinant protein has been studied as an alternative vaccine.
  
  
  CONCLUSIONS
  We designed a chimerical multi-epitope recombinant protein (5BT), which is comprised of tandem repeats of five B cell epitopes (residue of VP1 136-162) derived from different FMDV variants and one T-cell epitope (residue of 3A 21-35). To increase solubility and stability of 5BT, it was conjugated with BmpB, the membrane protein B of Brachyspira hyodysenteriae (B5BT). Our results indicated that 5BT was susceptible to degradation by host protease and produced with substantial fraction of inclusion body. The stability and solubility of 5BT was greatly increased by conjugating to BmpB. FMDV specific antibodies were observed in the serum of mice immunized with 5BT and B5BT comparable to inactivated FMD vaccine. Sera from 5BT and B5BT groups also exhibited high epitope-specific antibody titers in peptide specific ELISA, indicating that all five epitopes are exposed to the B cell receptor for the antibody reaction. Thus the multi-epitope recombinant protein designed in this study may be a potential candidate as an alternative vaccine against FMDV epidemic variants.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 115-921, Republic of Korea.
authors.forename: Ho-Bin
authors.initials: HB
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 115-921, Republic of Korea.
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Piao
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 115-921, Republic of Korea.
authors.forename: Jun-Yeong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 115-921, Republic of Korea.
authors.forename: Jae-Yun
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Institute of Green-Bio Science and Technology, Seoul National University, 1447-1 Pyeongchang-Daero, Daehwa-Myeon, Pyeongchang-Gun, Gangwon-Do, 25354, Republic of Korea.
authors.forename: Jin-Duck
authors.initials: JD
authors.lastname: Bok
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 115-921, Republic of Korea.
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
authors.affiliation: Institute of Green-Bio Science and Technology, Seoul National University, 1447-1 Pyeongchang-Daero, Daehwa-Myeon, Pyeongchang-Gun, Gangwon-Do, 25354, Republic of Korea. kangsk01@snu.ac.kr.
authors.forename: Sang-Kee
authors.initials: SK
authors.lastname: Kang
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 115-921, Republic of Korea. cyjcow@snu.ac.kr.|Research Institute for Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea. cyjcow@snu.ac.kr.
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry* / Q000276:immunology*; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D001425:Bacterial Outer Membrane Proteins; C415945:BmpB protein, Brachyspira hyodysenteriae; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D008074:Lipoproteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: Artificial recombinant protein; B cell epitope; FMDV; GH loop; Multi- epitope