title: Assessment of anti-cholinesterase activity and cytotoxicity of cagaita (Eugenia dysenterica) leaves.
abstract: Eugenia dysenterica ex DC Mart. (Myrtaceae) is a Brazilian tree with pharmacological and biological properties. The aqueous leaf extract, rich in polyphenols, was tested in the human neuroblastoma cell line SH-SY5Y to evaluate its effect on cell viability. The extract and two isolated compounds were also assessed for the potential inhibitory activity on acetylcholinesterase, an enzyme related to Alzheimer's disease. A simple chromatographic method using Sephadex LH-20 was developed to separate catechin and quercetin from the aqueous leaf extract of E. dysenterica. Identification was carried out by spectroscopic techniques IR, UV, and1H and13C NMR. The IC50values were obtained by constructing dose-response curves on a graph with percentage inhibition versus log of inhibitor concentration and compared with physostigmine, a well-known AChE inhibitor. The extract was toxic for SH-SY5Y cells at concentrations higher than 7.8 μg/ml given for 24 h. The decline in SH-SY5Y cell viability appears to be related to its antiproliferative activity. The extract also showed relatively moderate acetylcholinesterase inhibitory activity of 66.33% ± 0.52% at 1.0 mg/ml with an IC50value of 155.20 ± 2.09 μg/ml. Physostigmine, quercetin, and catechin showed IC50values of 18.69 ± 0.07, 46.59 ± 0.49, and 42.39 ± 0.67 μg/ml, respectively.
authors.affiliation: Faculty of Health Science, University of Brasilia, CEP 70910-900, Brasília DF, Brazil. Electronic address: cristiangasca25@gmail.com.
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Gasca
authors.affiliation: Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, CEP 14040-901, Ribeirão Preto, SP, Brazil.
authors.forename: Willian O
authors.initials: WO
authors.lastname: Castillo
authors.affiliation: Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, CEP 14040-901, Ribeirão Preto, SP, Brazil.
authors.forename: Catarina Satie
authors.initials: CS
authors.lastname: Takahashi
authors.affiliation: Faculdade de Ceilândia, University of Brasilia, CEP 70919-970, Brasília, Brazil.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Fagg
authors.affiliation: Faculty of Health Science, University of Brasilia, CEP 70910-900, Brasília DF, Brazil.
authors.forename: Pérola O
authors.initials: PO
authors.lastname: Magalhães
authors.affiliation: Faculty of Health Science, University of Brasilia, CEP 70910-900, Brasília DF, Brazil.
authors.forename: Yris M
authors.initials: YM
authors.lastname: Fonseca-Bazzo
authors.affiliation: Faculty of Health Science, University of Brasilia, CEP 70910-900, Brasília DF, Brazil.
authors.forename: Dâmaris
authors.initials: D
authors.lastname: Silveira
mesh_terms: D001938:Brazil; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002802:Cholinesterases / Q000737:chemistry; D000068856:Eugenia / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D002800:Cholinesterase Inhibitors; D010636:Phenols; D010936:Plant Extracts; D002802:Cholinesterases
keywords: Acetylcholinesterase; Alzheimer's disease; Catechin; Cerrado; Quercetin