title: Diversity of nodular bacteria of Scorpiurus muricatus in western Algeria and their impact on plant growth.
abstract: A total of 51 bacterial strains were isolated from root nodules of Scorpiurus muricatus sampled from 6 regions of western Algeria. Strain diversity was assessed by rep-PCR amplification fingerprinting, which grouped the isolates into 28 different clusters. Partial nucleotide sequencing of the 16S rRNA gene and BLAST analysis revealed that root nodules of S. muricatus were colonized by different species close to Rhizobium vignae, Rhizobium radiobacter, Rhizobium leguminosarum, Phyllobacterium ifriqiyense, Phyllobacterium endophyticum, Starkeya sp., and Pseudomonas sp. However, none of these strains was able to form nodules on its host plant; even nodC was present in a single strain (SMT8a). The inoculation test showed a great improvement in the growth of inoculated plants compared with noninoculated control plants. A significant amount of indole acetic acid was produced by some strains, but only 2 strains could solubilize phosphate. In this report we described for the first time the diversity of bacteria isolated from root nodules of S. muricatus growing in different regions in western Algeria and demonstrated their potential use in promoting plant growth.
authors.affiliation: a Laboratoire de Biotechnologie des Rhizobiums et Amélioration des Plantes, Université d'Oran1 Ahmed Ben Bella, Oran, Algérie.
authors.forename: Zoulikha
authors.initials: Z
authors.lastname: Bouchiba
authors.affiliation: a Laboratoire de Biotechnologie des Rhizobiums et Amélioration des Plantes, Université d'Oran1 Ahmed Ben Bella, Oran, Algérie.
authors.forename: Zineb Faiza
authors.initials: ZF
authors.lastname: Boukhatem
authors.affiliation: a Laboratoire de Biotechnologie des Rhizobiums et Amélioration des Plantes, Université d'Oran1 Ahmed Ben Bella, Oran, Algérie.
authors.forename: Zohra
authors.initials: Z
authors.lastname: Ighilhariz
authors.affiliation: a Laboratoire de Biotechnologie des Rhizobiums et Amélioration des Plantes, Université d'Oran1 Ahmed Ben Bella, Oran, Algérie.
authors.forename: Nouria
authors.initials: N
authors.lastname: Derkaoui
authors.affiliation: b Centre de Recherche Forestière, Agdal-Rabat, Maroc.
authors.forename: Benaissa
authors.initials: B
authors.lastname: Kerdouh
authors.affiliation: c Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des sciences, Université Mohammed V, Rabat, Maroc.
authors.forename: Hanaa
authors.initials: H
authors.lastname: Abdelmoumen
authors.affiliation: d Faculté Polydisciplinaire de Béni Mellal, Mghila B.P. 592, Université Sultan Moulay Slimane, Béni Mellal - Maroc.
authors.forename: Younes
authors.initials: Y
authors.lastname: Abbas
authors.affiliation: c Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des sciences, Université Mohammed V, Rabat, Maroc.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Missbah El Idrissi
authors.affiliation: a Laboratoire de Biotechnologie des Rhizobiums et Amélioration des Plantes, Université d'Oran1 Ahmed Ben Bella, Oran, Algérie.
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Bekki
mesh_terms: D000462:Algeria; D020561:Alphaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D007887:Fabaceae / Q000382:microbiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D010802:Phylogeny; D063245:Plant Development; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S; D012231:Rhizobium / Q000235:genetics / Q000302:isolation & purification
chemical_list: D007210:Indoleacetic Acids; D012336:RNA, Ribosomal, 16S; C030737:indoleacetic acid
keywords: RFCP; Scorpiurus muricatus; bactéries nodulaires; diversity; diversité; nodular bacterial; ouest de l’Algérie; plant-growth-promoting rhizobacteria; western Algeria