title: Transcriptomic Analysis of Male Black Tiger Shrimp (Penaeus monodon) After Polychaete Feeding to Enhance Testicular Maturation.
abstract: To reveal molecular mechanism of how polychaetes enhanced reproductive maturation in the male black tiger shrimp (Penaeus monodon), transcriptomic profiles of male reproductive organs (testes and vas deferens) between polychaete-fed and commercial pellet-fed male brooders were compared using cDNA microarray. The overall profiles were distinguishingly different between the two feed groups as well as between testes and vas deferens. Additionally, six of 11 differentially expressed gene identified by the microarray (HNRPUL1 and GCP4 in testes, MAT2B, CDC16, and CSN5 in vas deferens, and SLD5 in both organs) were validated by quantitative real-time PCR (qPCR) and found to exhibit significantly higher expression levels in polychaete-fed shrimp than those in commercial pellet-fed shrimp. From microarray and qPCR results, the differentially expressed transcripts in both testes and vas deferens between different feeds belonged to DNA replication and microtubule nucleation pathways. Interestingly, while the transcripts involved in nutrient uptake and nucleotide biosynthesis were increased only in testes, those involved in protein refolding and apoptosis were increased only in vas deferens. These findings suggest that polychaetes may enhance spermatogenesis by increasing spermatogonia proliferation in testes and by regulating mature spermatozoa in vas deferens.
authors.affiliation: Microarray Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Luang, Pathum Thani, Thailand. rungnapa.lee@biotec.or.th.
authors.forename: Rungnapa
authors.initials: R
authors.lastname: Leelatanawit
authors.affiliation: Microarray Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Luang, Pathum Thani, Thailand.
authors.forename: Umaporn
authors.initials: U
authors.lastname: Uawisetwathana
authors.affiliation: Microarray Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Luang, Pathum Thani, Thailand.
authors.forename: Amornpan
authors.initials: A
authors.lastname: Klanchui
authors.affiliation: Shrimp Genetic Improvement Center (SGIC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Surat Thani, Thailand.
authors.forename: Jutatip
authors.initials: J
authors.lastname: Khudet
authors.affiliation: Shrimp Genetic Improvement Center (SGIC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Surat Thani, Thailand.
authors.forename: Suwanchai
authors.initials: S
authors.lastname: Phomklad
authors.affiliation: Shrimp Genetic Improvement Center (SGIC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Surat Thani, Thailand.
authors.forename: Somjai
authors.initials: S
authors.lastname: Wongtriphop
authors.affiliation: Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Luang, Pathum Thani, Thailand.
authors.forename: Pikul
authors.initials: P
authors.lastname: Jiravanichpaisal
authors.affiliation: Microarray Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Luang, Pathum Thani, Thailand.
authors.forename: Nitsara
authors.initials: N
authors.lastname: Karoonuthaisiri
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D017209:Apoptosis; D004247:DNA / Q000096:biosynthesis; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D033561:Penaeidae / Q000235:genetics / Q000254:growth & development*; D011077:Polychaeta; D013737:Testis / Q000254:growth & development / Q000378:metabolism; D014649:Vas Deferens / Q000254:growth & development / Q000378:metabolism
chemical_list: D004247:DNA
keywords: Penaeus monodon; Polychaetes; Quantitative real-time PCR; Testes; Vas deferens; cDNA microarray