title: Cross reactivity of commercial anti-dengue immunoassays in patients with acute Zika virus infection.
abstract: Several countries have local transmission of multiple arboviruses, in particular, dengue and Zika viruses, which have recently spread through many American countries. Cross reactivity among Flaviviruses is high and present a challenge for accurate identification of the infecting agent. Thus, we evaluated the level of cross reactivity of anti-dengue IgM/G Enzyme-Linked Immunosorbent Assays (ELISA) from three manufacturers against 122 serum samples obtained at two time-points from 61 patients with non-dengue confirmed Zika virus infection. All anti-dengue ELISAs cross reacted with serum from patients with acute Zika infection at some level and a worrisome number of seroconversion for dengue IgG and IgM was observed. These findings may impact the interpretation of currently standard criteria for dengue diagnosis in endemic regions.
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Alvina Clara
authors.initials: AC
authors.lastname: Felix
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Nathalia C Santiago
authors.initials: NCS
authors.lastname: Souza
authors.affiliation: Serviço Especial de Saúde de Araraquara-SESA, Faculdade de Saúde Pública da USP, São Paulo, Brazil.
authors.forename: Walter M
authors.initials: WM
authors.lastname: Figueiredo
authors.affiliation: Serviço Especial de Saúde de Araraquara-SESA, Faculdade de Saúde Pública da USP, São Paulo, Brazil.
authors.forename: Angela A
authors.initials: AA
authors.lastname: Costa
authors.affiliation: Serviço Especial de Saúde de Araraquara-SESA, Faculdade de Saúde Pública da USP, São Paulo, Brazil.
authors.forename: Marta
authors.initials: M
authors.lastname: Inenami
authors.affiliation: Serviço Especial de Saúde de Araraquara-SESA, Faculdade de Saúde Pública da USP, São Paulo, Brazil.
authors.forename: Rosangela M G
authors.initials: RMG
authors.lastname: da Silva
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Levi
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Claudio Sergio
authors.initials: CS
authors.lastname: Pannuti
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Camila Malta
authors.identifier: 0000-0003-4550-1987
authors.initials: CM
authors.lastname: Romano
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D003429:Cross Reactions; D003715:Dengue / Q000175:diagnosis*; D003951:Diagnostic Errors; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D014777:Virus Diseases; D000071243:Zika Virus Infection / Q000175:diagnosis*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: Zika virus; cross reaction; dengue virus; serological tests