title: Periostin antisense oligonucleotide prevents adhesion formation after surgery in mice.
abstract: To study the role of periostin in adhesion formation, the effect of periostin antisense oligonucleotide (PAO) on adhesion formation was evaluated in mice. Under anesthesia, the serous membrane of the cecum was abraded, and the adhesion score and mRNA levels of periostin and its related factors were determined after surgery. Saline, 40 mg/kg of negative sense oligonucleotide (NSO), or 40 mg/kg of PAO were injected into the abdomen after surgery, and the adhesion score and mRNA levels were evaluated 14 days later. Filmy adhesion formation was observed 1 day after surgery, and the adhesion score increased gradually to 14 days. The mRNA levels of periostin, transforming growth factor (TGF)-β, and collagen I increased gradually from 3 days to 14 days. The adhesion score of PAO was significantly lower than of saline or NSO 14 days after surgery. The mRNA levels of periostin, TGF-β, and collagen I were also significantly attenuated by treatment with PAO compared with saline or NSO. Thus, these results demonstrated that the periostin mRNA level increased in the abraded cecum, and PAO prevented adhesion formation along with attenuation of the periostin mRNA level.
authors.affiliation: Department of Innovative Medicine, Osaka Medical College Graduate School of Medicine, Takatsuki, Japan. Electronic address: pha010@osaka-med.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Takai
authors.affiliation: AQUA Therapeutics Co., Ltd., Kobe, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yoshino
authors.affiliation: AQUA Therapeutics Co., Ltd., Kobe, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Takao
authors.affiliation: AQUA Therapeutics Co., Ltd., Kobe, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Department of Innovative Medicine, Osaka Medical College Graduate School of Medicine, Takatsuki, Japan.
authors.forename: Denan
authors.initials: D
authors.lastname: Jin
mesh_terms: D000818:Animals; D002432:Cecum / Q000473:pathology / Q000601:surgery*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D012704:Serous Membrane / Q000293:injuries*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D016376:Oligonucleotides, Antisense; C082838:Postn protein, mouse; D016212:Transforming Growth Factor beta
keywords: Adhesion; Antisense; Mice; Periostin; Surgery