title: Overexpression of hsa-miR-939 follows by NGFR down-regulation and apoptosis reduction.
abstract: Neurotrophin receptors play a crucial role in neuronal survival, differentiation and regeneration. Nerve growth factor receptor (NGFR) or P75NTRis a neurotrophin receptor that is involved in many pathological conditions including cancers. Genetic factors that are involved in regulation of neurotrophin receptors are under intense investigation. MiRNAs are novel regulators of signalling pathways that are candidates for regulation of neurotrophin receptors. Computational programs predicted that NGFR gene is a bona fide target for hsa-miR- 939. RT-qPCR, Western analysis and dual luciferase assay evidences indicated that NGFR transcript is targeted by hsa-miR-939. Also, hsa-miR-939 overexpression brought about down-regulation of NGFR expression in U87 cell line, followed by cell death rate reduction, detected by flow cytometry. Taken together, here for the first time, hsa-miR-939 is introduced as a novel key regulator of NGFR expression and its involvement in cell death/survival processes is suggested.
authors.affiliation: Molecular Genetics Department, Faculty of Biological Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Fahimeh Hosseini
authors.initials: FH
authors.lastname: Aghdaei
authors.forename: Bahram M
authors.initials: BM
authors.lastname: Soltani
authors.forename: Sadat
authors.initials: S
authors.lastname: Dokanehiifard
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Mowla
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics*; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C000604729:MIRN939 microRNA, human; D035683:MicroRNAs; C484065:NGFR protein, human; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor