title: Anticancer Effects ofSalvia miltiorrhizaAlcohol Extract on Oral Squamous Carcinoma Cells.
abstract: Researchers have reported significant effects from Danshen (Salvia miltiorrhiza) in terms of inhibiting tumor cell proliferation and promoting apoptosis in breast cancer, hepatocellular carcinomas, promyelocytic leukemia, and clear cell ovary carcinomas. Here we report our data indicating that Danshen extracts, especially alcohol extract, significantly inhibited the proliferation of the human oral squamous carcinoma (OSCC) cell lines HSC-3 and OC-2. We also observed that Danshen alcohol extract activated the caspase-3 apoptosis executor by impeding members of the inhibitor of apoptosis (IAP) family, but not by regulating the Bcl-2-triggered mitochondrial pathway in OSCC cells. Our data also indicate that the extract exerted promising effects in vivo, with HSC-3 tumor xenograft growth being suppressed by 40% and 69% following treatment with Danshen alcohol extract at 50 and 100 mg/kg, respectively, for 34 days. Combined, our results indicate appreciable anticancer activity and significant potential for Danshen alcohol extract as a natural antioxidant and herbal human oral cancer chemopreventive drug.
authors.affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.
authors.forename: Wen-Hung
authors.identifier: 0000-0002-2334-5621
authors.initials: WH
authors.lastname: Wang
authors.affiliation: Department of Hemato-Oncology, Chi-Mei Medical Center, Tainan City 710, Taiwan.
authors.forename: Kuo-Yu
authors.initials: KY
authors.lastname: Hsuan
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Ling-Ya
authors.initials: LY
authors.lastname: Chu
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chia-Ying
authors.initials: CY
authors.lastname: Lee
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Yu-Chang
authors.identifier: 0000-0002-1917-5893
authors.initials: YC
authors.lastname: Tyan
authors.affiliation: Institute of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan City 717, Taiwan.
authors.forename: Zong-Shiow
authors.initials: ZS
authors.lastname: Chen
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Wan-Chi
authors.identifier: 0000-0002-4490-3581
authors.initials: WC
authors.lastname: Tsai