title: Bone marrow mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke in type 2 diabetic rats.
abstract: Our previous studies have found that stem cells conditioned medium (CM) facilitated functional recovery after stroke in non-diabetics. However, whether bone marrow stromal cells conditioned medium (BMSCs-CM) treatment after stroke in type 2 diabetic (T2DM) rats improve functional outcomes remains unclear. T2DM rats were induced and subjected to stroke then treated with or without BMSCs-CM. Functional outcomes and blood-brain barrier (BBB) leakage were performed, the expression of Angiopoietin (Ang) 1 and tyrosine kinase (Tie) 2 were also assessed. Our results showed that BMSCs-CM treatment significantly improved functional outcomes, decreased BBB leakage and the expression of Ang1 and Tie2 were also changed after BMSCs-CM treatment in type 2 diabetes after stroke. In conclusion, enhanced expression of Ang1 and Tie2 in ischemic brain after BMSCs-CM treatment of stroke may contribute to the improved functional recovery after stroke in type 2 diabetic rats.
authors.affiliation: Department of Rehabilitation, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Shen
authors.affiliation: Department of Geriatric Neurology, Nanjing Brain Hospital, Affiliated to Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Hua
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zan
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zu
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China. Electronic address: guiyuncui@foxmail.com.
authors.forename: Guiyun
authors.initials: G
authors.lastname: Cui
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China. Electronic address: xinchunye@gmail.com.
authors.forename: Xinchun
authors.initials: X
authors.lastname: Ye
mesh_terms: D042683:Angiopoietin-1 / Q000096:biosynthesis; D000818:Animals; D001854:Bone Marrow Cells; D001921:Brain / Q000378:metabolism / Q000473:pathology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D003924:Diabetes Mellitus, Type 2; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D042787:Receptor, TIE-2 / Q000096:biosynthesis; D020127:Recovery of Function / Q000187:drug effects; D020521:Stroke / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D066253:Vascular Remodeling
chemical_list: D042683:Angiopoietin-1; D017077:Culture Media, Conditioned; D042787:Receptor, TIE-2
keywords: Angiopoietin 1; Bone marrow stromal cells; Ischemic stroke; Type 2 diabetes mellitus; Tyrosine kinase 2