title: Configurations of the Re-scan Confocal Microscope (RCM) for biomedical applications.
abstract: The new high-sensitive and high-resolution technique, Re-scan Confocal Microscopy (RCM), is based on a standard confocal microscope extended with a re-scan detection unit. The re-scan unit includes a pair of re-scanning mirrors that project the emission light onto a camera in a scanning manner. The signal-to-noise ratio of Re-scan Confocal Microscopy is improved by a factor of 4 compared to standard confocal microscopy and the lateral resolution of Re-scan Confocal Microscopy is 170 nm (compared to 240 nm for diffraction limited resolution, 488 nm excitation, 1.49 NA). Apart from improved sensitivity and resolution, the optical setup of Re-scan Confocal Microscopy is flexible in its configuration in terms of control of the mirrors, lasers and filters. Because of this flexibility, the Re-scan Confocal Microscopy can be configured to address specific biological applications. In this paper, we explore a number of possible configurations of Re-scan Confocal Microscopy for specific biomedical applications such as multicolour, FRET, ratio-metric (e.g. pH and intracellular Ca2+measurements) and FRAP imaging.
authors.affiliation: Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: G M R
authors.initials: GMR
authors.lastname: DE Luca
authors.affiliation: Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Desclos
authors.affiliation: Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: R M P
authors.initials: RMP
authors.lastname: Breedijk
authors.affiliation: Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Dolz-Edo
authors.affiliation: Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Smits
authors.affiliation: Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Nahidiazar
authors.affiliation: Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Bielefeld
authors.affiliation: Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Picavet
authors.affiliation: Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands.
authors.forename: C P
authors.initials: CP
authors.lastname: Fitzsimons
authors.affiliation: Van Leeuwenhoek Centre for Advanced Microscopy, Department of Cell Biology and Histology, Academic Medical Centre, Amsterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Hoebe
authors.affiliation: Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.|Nikon Centre of Excellence on Super Resolution Microscopy Development, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: E M M
authors.initials: EMM
authors.lastname: Manders
mesh_terms: D000818:Animals; D002460:Cell Line; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*
keywords: High-sensitive microscopy; live cell imaging; multicolour microscopy; super-resolution microscopy