title: Effect of sodium selenite on isoflavonoid contents and antioxidant capacity of chickpea (Cicer arietinum L.) sprouts.
abstract: Isoflavonoid compositions, phenylalanine ammonia lyase (PAL) activity and antioxidant capacity were evaluated in chickpea (Cicer arietinum L.) sprouts germinated after soaking with different sodium selenite (Na2SeO3) concentrations (0, 1 and 2mg/100g seeds). Chickpea seeds were germinated during four days at 24°C and the isoflavonoid profiles and concentrations evaluated by HPLC-UV daily during four days of germination. Eleven isoflavones and two pterocarpan phytoalexins forms were identified in sprouts, being malonylated formononetin glycoside, formononetin, isoformononetin glycoside and malonylated biochanin A glycoside the major compounds. Compared to untreated sprouts, total isoflavonoid, PAL activity and antioxidant capacity showed a remarkable increase of 83%, 56%, and 33%, respectively in chickpea sprouts that were treated with a high sodium selenite content (2mg/100g seeds). Results suggest that Se-enriched chickpea sprouts could represent a good source of dietary Se and as an upgraded source of isoflavonoids.
authors.affiliation: Programa Regional de Posgrado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, FCQB-UAS, AP 1354, CP 80000 Culiacán, Sinaloa, Mexico.
authors.forename: Daniela
authors.initials: D
authors.lastname: Guardado-Félix
authors.affiliation: Tecnologico de Monterrey, Centro de Biotecnología FEMSA, Escuela de Ingeniería y Ciencias, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849 Monterrey, NL, Mexico.
authors.forename: Sergio O
authors.initials: SO
authors.lastname: Serna-Saldivar
authors.affiliation: Programa Regional de Posgrado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, FCQB-UAS, AP 1354, CP 80000 Culiacán, Sinaloa, Mexico.
authors.forename: Edith O
authors.initials: EO
authors.lastname: Cuevas-Rodríguez
authors.affiliation: Tecnologico de Monterrey, Centro de Biotecnología FEMSA, Escuela de Ingeniería y Ciencias, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849 Monterrey, NL, Mexico.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Jacobo-Velázquez
authors.affiliation: Tecnologico de Monterrey, Centro de Biotecnología FEMSA, Escuela de Ingeniería y Ciencias, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849 Monterrey, NL, Mexico. Electronic address: jagu@itesm.mx.
authors.forename: Janet A
authors.initials: JA
authors.lastname: Gutiérrez-Uribe
mesh_terms: D000975:Antioxidants; D029863:Cicer / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D018506:Gene Expression Regulation, Plant; D007529:Isoflavones; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics*; D036226:Seedlings / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D012717:Sesquiterpenes; D018038:Sodium Selenite / Q000494:pharmacology*; D000094162:Phytoalexins
chemical_list: D000975:Antioxidants; D007529:Isoflavones; D012717:Sesquiterpenes; D010650:Phenylalanine Ammonia-Lyase; D018038:Sodium Selenite; D000094162:Phytoalexins
keywords: Cicer arietinum L.; Germination; Isoflavonoids; Selenium