title: Protective effect of an astaxanthin nanoemulsion against neomycin-induced hair-cell damage in zebrafish.
abstract: The cause of those hair cells disorder is oxidative stress due to free radicals. In particular, hair cells are very susceptible to aminoglycosides. Antioxidant is known to reduce the generation of oxygen-derived free radicals. Various antioxidant is marketed, and different dosage form is developed with the same drug. Using a zebra fish lateral line, we report hair cell protection effect of astaxanthin from neomycin-induced trauma.
  
  
  
  Zebrafish larvae were exposed to the astaxanthin nanoemulsion or to the suspension for 1 h, or were left unexposed. Subsequently, the larvae were exposed to neomycin for 1 h by adding the neomycin solution. Results were calculated as the mean hair-cell survival as a percentage of the control.
  
  
  
  Hair cells were not protected in the group treated with astaxanthin suspension and neomycin. On the other hand, there was dose-dependent protection against neomycin-induced hair-cell death in the zebrafish lateral-line hair cells in the group treated with nano astaxanthin.
  
  
  
  The results of the current study performed using a zebra fish lateral-line, nano astaxanthin protected sensory hair cells against neomycin-induced death. This suggests that nano-astaxanthin is more efficiently absorbed in the body than astaxanthin, and may be useful as a protective drug for the inner ear.
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Takemoto
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hirose
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sugahara
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Hara
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan. Electronic address: hiro-shi@yamaguchi-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D049329:Nanostructures; D009355:Neomycin / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D024341:Xanthophylls / Q000494:pharmacology; D015027:Zebrafish
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D004655:Emulsions; D017382:Reactive Oxygen Species; D024341:Xanthophylls; C005948:astaxanthine; D009355:Neomycin
keywords: Hair cell; Nanoemulsion; Oxidation; Screening; Zebrafish