title: Simultaneous fingerprint, quantitative analysis and anti-oxidative based screening of components in Rhizoma Smilacis Glabrae using liquid chromatography coupled with Charged Aerosol and Coulometric array Detection.
abstract: An analytical approach including fingerprint, quantitative analysis and rapid screening of anti-oxidative components was established and successfully applied for the comprehensive quality control of Rhizoma Smilacis Glabrae (RSG), a well-known Traditional Chinese Medicine with the homology of medicine and food. Thirteen components were tentatively identified based on their retention behavior, UV absorption and MS fragmentation patterns. Chemometric analysis based on coulmetric array data was performed to evaluate the similarity and variation between fifteen batches. Eight discriminating components were quantified using single-compound calibration. The unit responses of those components in coulmetric array detection were calculated and compared with those of several compounds reported to possess antioxidant activity, and four of them were tentatively identified as main contributors to the total anti-oxidative activity. The main advantage of the proposed approach was that it realized simultaneous fingerprint, quantitative analysis and screening of anti-oxidative components, providing comprehensive information for quality assessment of RSG.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China. Electronic address: diabloyg@126.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, No. 24 Tong Jia Xiang, Nanjing 210009, PR China. Electronic address: yoyo0132@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China. Electronic address: wenjunapple@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wen
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China. Electronic address: tingting_zoo@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China; Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, No. 100 Haining Road, Shanghai 200025, PR China; School of Medicine, Tongji University, No. 1239 Siping Road, Shanghai 200092, PR China. Electronic address: guorfan@outlook.com.
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D019668:Liliaceae / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal
keywords: Accelerated solvent extraction; Charged aerosol detection; Coulometric array detection; Mass spectrometry; Rhizoma Smilacis Glabrae