title: Non-destructive monitoring of mouse embryo development and its qualitative evaluation at the molecular level using Raman spectroscopy.
abstract: Current research focuses on embryonic development and quality not only by considering fundamental biology, but also by aiming to improve assisted reproduction technologies, such as in vitro fertilization. In this study, we explored the development of mouse embryo and its quality based on molecular information, obtained nondestructively using Raman spectroscopy. The detailed analysis of Raman spectra measured in situ during embryonic development revealed a temporary increase in protein content after fertilization. Proteins with a β-sheet structure-present in the early stages of embryonic development-are derived from maternal oocytes, while α-helical proteins are additionally generated by switching on a gene after fertilization. The transition from maternal to embryonic control during development can be non-destructively profiled, thus facilitating the in situ assessment of structural changes and component variation in proteins generated by metabolic activity. Furthermore, it was indicated that embryos with low-grade morphology had high concentrations of lipids and hydroxyapatite. This technique could be used for embryo quality testing in the future.
authors.affiliation: Department of Chemistry, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.|Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Ishigaki
authors.affiliation: Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Hashimoto
authors.affiliation: Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sato
authors.affiliation: Department of Chemistry, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Ozaki
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000737:chemistry*; D047108:Embryonic Development / Q000502:physiology; D005307:Fertilization in Vitro; D051379:Mice; D008813:Mice, Inbred ICR; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman