title: "Click" chemistry mediated construction of cationic curdlan nanocarriers for efficient gene delivery.
abstract: A cationic group has been quantitatively and selectively introduced into C6 position of each glucose units of Curdlan by "Click Chemistry" successfully. The resulting cationic Curdlan-Imidazole-lysine polymers (Cur-6-100Lys) exhibit excellent water solubility. Structure of the Cur-6-100Lys complexes was verified by FTIR and NMR spectroscopic measurements, and analysis of Cur-6-100Lys by GPC, DLS and SEM revealed that they have stoichiometric, nanosized spheroidal structures. Cytotoxicity measurement, electrophoretic mobility shift assay and EGFP-pDNA transfection have been carried out respectively. The results clearly show that Cur-6-100Lys nanocarriers have bound to dsDNA promptly, are less cytotoxic to both 7901 cells and HeLa cells, and are readily able to transport EGFP-pDNA into HepG2 cells. Our studies indicated that Cur-6-100Lys can potentially be used as a versatile nano platform for efficient gene delivery in living cells.
authors.affiliation: School of Chemistry & Chemical Engineering, Inner Mongolia University, 235 West College Road, Hohhot, Inner Mongolia 010020, PR China.
authors.forename: Jingfen
authors.initials: J
authors.lastname: Han
authors.affiliation: School of Chemistry & Chemical Engineering, Inner Mongolia University, 235 West College Road, Hohhot, Inner Mongolia 010020, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Chemistry & Chemical Engineering, Inner Mongolia University, 235 West College Road, Hohhot, Inner Mongolia 010020, PR China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Chemistry & Chemical Engineering, Inner Mongolia University, 235 West College Road, Hohhot, Inner Mongolia 010020, PR China.
authors.forename: Dongxue
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Chemistry & Chemical Engineering, Inner Mongolia University, 235 West College Road, Hohhot, Inner Mongolia 010020, PR China.
authors.forename: Suyaola
authors.initials: S
authors.lastname: Suyaola
authors.affiliation: School of Chemistry & Chemical Engineering, Inner Mongolia University, 235 West College Road, Hohhot, Inner Mongolia 010020, PR China.
authors.forename: Tianyue
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Chemistry & Chemical Engineering, Inner Mongolia University, 235 West College Road, Hohhot, Inner Mongolia 010020, PR China. Electronic address: hbaigude@imu.edu.cn.
authors.forename: Huricha
authors.initials: H
authors.lastname: Baigude
mesh_terms: D002412:Cations; D002470:Cell Survival; D057930:Click Chemistry; D005822:Genetic Vectors / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles; D010957:Plasmids; D014162:Transfection; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D002412:Cations; D047071:beta-Glucans; C038459:curdlan
keywords: Click reaction; Curdlan; Gene carrier; Gene delivery; Lysine