title: Thermodynamics of globular proteins.
abstract: The analysis of temperature-induced unfolding of proteins in aqueous solutions was performed. Based on the data of thermodynamic parameters of protein unfolding and using the method of semi-empirical calculations of hydration parameters at reference temperature 298 K, we obtained numerical values of enthalpy, free energy, and entropy which characterize the unfolding of proteins in the 'gas phase'. It was shown that specific values of the energy of weak intramolecular bonds (∆Hint), conformational free energy (∆Gconf) and entropy (∆Sconf) are the same for proteins with molecular weight 7-25 kDa. Using the energy value (∆Hint) and the proposed approach for estimation of the conformational entropy of native protein (SNC), numerical values of the absolute free energy (GNC) were obtained.
authors.affiliation: a Institute of Cell Biophysics, Russian Academy of Sciences , Pushchino , Moscow Region 142290 , Russia.
authors.forename: Nikolay N
authors.initials: NN
authors.lastname: Khechinashvili
authors.affiliation: a Institute of Cell Biophysics, Russian Academy of Sciences , Pushchino , Moscow Region 142290 , Russia.
authors.forename: Artem V
authors.initials: AV
authors.lastname: Kabanov
authors.affiliation: a Institute of Cell Biophysics, Russian Academy of Sciences , Pushchino , Moscow Region 142290 , Russia.
authors.forename: Maxim S
authors.initials: MS
authors.lastname: Kondratyev
authors.affiliation: b Institute of Theoretical and Experimental Biophysics , Russian Academy of Sciences , Pushchino , Moscow Region 142290 , Russia.
authors.forename: Robert V
authors.initials: RV
authors.lastname: Polozov
mesh_terms: D019277:Entropy; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
chemical_list: D011506:Proteins; D014867:Water
keywords: absolute free energy; conformational entropy; protein energetics; protein unfolding