title: Diversity Profile and Dynamics of Peptaibols Produced by Green Mould Trichoderma Species in Interactions with Their Hosts Agaricus bisporus and Pleurotus ostreatus.
abstract: Certain Trichoderma species are causing serious losses in mushroom production worldwide. Trichoderma aggressivum and Trichoderma pleuroti are among the major causal agents of the green mould diseases affecting Agaricus bisporus and Pleurotus ostreatus, respectively. The genus Trichoderma is well-known for the production of bioactive secondary metabolites, including peptaibols, which are short, linear peptides containing unusual amino acid residues and being synthesised via non-ribosomal peptide synthetases (NRPSs). The aim of this study was to get more insight into the peptaibol production of T. aggressivum and T. pleuroti. HPLC/MS-based methods revealed the production of peptaibols closely related to hypomurocins B by T. aggressivum, while tripleurins representing a new group of 18-residue peptaibols were identified in T. pleuroti. Putative NRPS genes enabling the biosynthesis of the detected peptaibols could be found in the genomes of both Trichoderma species. In vitro experiments revealed that peptaibols are potential growth inhibitors of mushroom mycelia, and that the host mushrooms may have an influence on the peptaibol profiles of green mould agents.
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Marik
authors.affiliation: Department of General and Environmental Microbiology, Faculty of Sciences, and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624, Pécs, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Urbán
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary.
authors.forename: Chetna
authors.initials: C
authors.lastname: Tyagi
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Szekeres
authors.affiliation: Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, H-6726, Szeged, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Leitgeb
authors.affiliation: Institute of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720, Szeged, Hungary.
authors.forename: Máté
authors.initials: M
authors.lastname: Vágvölgyi
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Manczinger
authors.affiliation: Research Area Biochemical Technology, Institute of Chemical and Biological Engineering, TU Wien, Getreidemarkt 9/166, A-1060, Vienna, Austria.
authors.forename: Irina S
authors.initials: IS
authors.lastname: Druzhinina
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Kredics
mesh_terms: D000363:Agaricales / Q000187:drug effects / Q000254:growth & development*; D000364:Agaricus; D005800:Genes, Fungal; D016681:Genome, Fungal; D006131:Growth Inhibitors; D009181:Mycoses; D054848:Peptaibols / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D020076:Pleurotus; D014242:Trichoderma / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D006131:Growth Inhibitors; D054848:Peptaibols
keywords: Cultivated mushrooms; Liquid chromatography; Mass spectrometry; Peptaibol; Trichoderma green mould