title: Antioxidant Properties and Cardioprotective Mechanism of Malaysian Propolis in Rats.
abstract: Propolis contains high concentrations of polyphenols, flavonoids, tannins, ascorbic acid, and reducing sugars and proteins. Malaysian Propolis (MP) has been reported to exhibit high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing antioxidant power (FRAP) values. Herein, we report the antioxidant properties and cardioprotective properties of MP in isoproterenol- (ISO-) induced myocardial infarction in rats. Male Wistar rats (n= 32) were pretreated orally with an ethanol extract of MP (100 mg/kg/day) for 30 consecutive days. Subcutaneous injection of ISO (85 mg/kg in saline) for two consecutive days caused a significant increase in serum cardiac marker enzymes and cardiac troponin I levels and altered serum lipid profiles. In addition significantly increased lipid peroxides and decreased activities of cellular antioxidant defense enzymes were observed in the myocardium. However, pretreatment of ischemic rats with MP ameliorated the biochemical parameters, indicating the protective effect of MP against ISO-induced ischemia in rats. Histopathological findings obtained for the myocardium further confirmed the biochemical findings. It is concluded that MP exhibits cardioprotective activity against ISO-induced oxidative stress through its direct cytotoxic radical-scavenging activities. It is also plausible that MP contributed to endogenous antioxidant enzyme activity via inhibition of lipid peroxidation.
authors.affiliation: Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
authors.forename: Romana
authors.initials: R
authors.lastname: Ahmed
authors.affiliation: Veterinary Drug Residue Analysis Division, Institute of Food & Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka 1349, Bangladesh.
authors.forename: E M
authors.identifier: 0000-0001-5867-7004
authors.initials: EM
authors.lastname: Tanvir
authors.affiliation: Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
authors.forename: Md Sakib
authors.initials: MS
authors.lastname: Hossen
authors.affiliation: Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
authors.forename: Rizwana
authors.identifier: 0000-0003-1108-3406
authors.initials: R
authors.lastname: Afroz
authors.affiliation: Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
authors.forename: Istiyak
authors.initials: I
authors.lastname: Ahmmed
authors.affiliation: Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
authors.forename: Nur-E-Noushin
authors.initials: NE
authors.lastname: Rumpa
authors.affiliation: Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.
authors.forename: Sudip
authors.identifier: 0000-0003-1448-2269
authors.initials: S
authors.lastname: Paul
authors.affiliation: Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Siew Hua
authors.identifier: 0000-0001-6470-3651
authors.initials: SH
authors.lastname: Gan
authors.affiliation: Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
authors.forename: Siti Amrah
authors.initials: SA
authors.lastname: Sulaiman
authors.affiliation: Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh; Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Md Ibrahim
authors.identifier: 0000-0002-8358-0252
authors.initials: MI
authors.lastname: Khalil