title: Improved method for the extraction and chromatographic analysis on a fused-core column of ellagitannins found in oak-aged wine.
abstract: A new method for the analysis of ellagitannins observed in oak-aged wine is proposed, exhibiting interesting advantages with regard to previously reported analytical methods. The necessary extraction of ellagitannins from wine was simplified to a single step of solid phase extraction (SPE) using size exclusion chromatography with Sephadex LH-20 without the need for any previous SPE of phenolic compounds using reversed-phase materials. The quantitative recovery of wine ellagitannins requires a combined elution with methanol and ethyl acetate, especially for increasing the recovery of the less polar acutissimins. The chromatographic method was performed using a fused-core C18 column, thereby avoiding the coelution of main ellagitannins, such as vescalagin and roburin E. However, the very polar ellagitannins, namely, the roburins A, B and C, still partially coeluted, and their quantification was assisted by the MS detector. This methodology also enabled the analysis of free gallic and ellagic acids in the same chromatographic run.
authors.affiliation: Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, C/Marcel.li Domingo, s/n, 43007 Tarragona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Navarro
authors.affiliation: Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, C/Marcel.li Domingo, s/n, 43007 Tarragona, Spain.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Kontoudakis
authors.affiliation: Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, C/Marcel.li Domingo, s/n, 43007 Tarragona, Spain.
authors.forename: Joan Miquel
authors.initials: JM
authors.lastname: Canals
authors.affiliation: Instituto de la Vid y el Vino de Castilla-La Mancha, Ctra. Toledo-Albacete s/n, 13700 Tomelloso, Ciudad Real, Spain.
authors.forename: Esteban
authors.initials: E
authors.lastname: García-Romero
authors.affiliation: Universidad de Castilla-La Mancha, Instituto Regional de Investigación Científica Aplicada, Avda. Camilo José Cela s/n, Campus Universitario, 13071 Ciudad Real, Spain; Parque Científico y Tecnológico Castilla-La Mancha, Paseo de la Innovación, 1, 02006 Albacete, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Gómez-Alonso
authors.affiliation: Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, C/Marcel.li Domingo, s/n, 43007 Tarragona, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Zamora
authors.affiliation: Universidad de Castilla-La Mancha, Instituto Regional de Investigación Científica Aplicada, Avda. Camilo José Cela s/n, Campus Universitario, 13071 Ciudad Real, Spain. Electronic address: isidro.hermosin@uclm.es.
authors.forename: Isidro
authors.initials: I
authors.lastname: Hermosín-Gutiérrez
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D003911:Dextrans; D005511:Food Handling; D047348:Hydrolyzable Tannins / Q000032:analysis / Q000302:isolation & purification*; D029963:Quercus / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D003911:Dextrans; D047348:Hydrolyzable Tannins; C025614:sephadex
keywords: Acutissimin; Castalagin; Ellagitannins; Fused-core; Grandinin; Roburin; Vescalagin; Wine aging; acutissimin A (CAS No. 108906-66-7: PubChem ID: 101676951); acutissimin B (CAS No. 108906-65-6: PubChem ID: 101676953); castalagin (CAS No. 24312-00-3: PubChem CID: 12302513); grandinin (CAS No. 115166-32-0: PubChem CID: 492392); roburin A (CAS No. 132864-75-6: PubChem CID: 101670390); roburin B (PubChem CID: 101670611); roburin C (PubChem CID: 101670612); roburin D (CAS No. 136199-93-4: PubChem CID: 101998420); roburin E (PubChem CID: 101670389); vescalagin (CAS No. 36001-47-5: PubChem CID: 5458626)