title: Collagen-based silver nanoparticles: Study on cell viability, skin permeation, and swelling inhibition.
abstract: Collagen is considered the most abundant protein in the animal kingdom, comprising 30% of the total amount of proteins and 6% of the human body by weight. Studies that examine the interaction between silver nanoparticles and proteins have been highlighted in the literature in order to understand the stability of the nanoparticle system, the effects observed in biological systems, and the appearance of new chemical pharmaceutical products. The objective of this study was to analyze the behavior of silver nanoparticles stabilized with collagen (AgNPcol) and to check the skin permeation capacity and action in paw edema induced by carrageenan. AgNPcol synthesis was carried out using solutions of reducing agent sodium borohydride (NaBH4), silver nitrate (AgNO3) and collagen. Characterization was done by using dynamic light scattering (DLS) and X-ray diffraction (XRD) and AFM. Cellular viability testing was performed by using flow cytometry in human melanoma cancer (MV3) and murine fibroblast (L929) cells. The skin permeation study was conducted using a Franz diffusion cell, and the efficiency of AgNPcol against the formation of paw edema in mice was evaluated. The hydrodynamic diameter and zeta potential of AgNPcol were 140.7±7.8nm and 20.1±0.7mV, respectively. AgNPcol failed to induce early apoptosis, late apoptosis, and necrosis in L929 cells; however, it exhibited enhanced toxicity in cancer cells (MV3) compared to normal cells (L929). AgNPcol demonstrated increased toxicological effects in cancer MV3 cells, promoting skin permeation, and preventing paw edema.
authors.affiliation: Research Center in Biodiversity and Biotechnology, Biotec, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil; Physiotherapy Department, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil. Electronic address: vscfisio@ufpi.edu.br.
authors.forename: Vinicius Saura
authors.initials: VS
authors.lastname: Cardoso
authors.affiliation: Physiotherapy Department, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil; Morphology and Muscle Physiology Laboratory, LAMFIM, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Carvalho Filgueiras
authors.affiliation: Physiotherapy Department, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil; Morphology and Muscle Physiology Laboratory, LAMFIM, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil.
authors.forename: Yago Medeiros
authors.initials: YM
authors.lastname: Dutra
authors.affiliation: Physiotherapy Department, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil; Morphology and Muscle Physiology Laboratory, LAMFIM, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil.
authors.forename: Ramon Handerson Gomes
authors.initials: RHG
authors.lastname: Teles
authors.affiliation: Research Center in Biodiversity and Biotechnology, Biotec, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil.
authors.forename: Alyne Rodrigues
authors.initials: AR
authors.lastname: de Araújo
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP, Universidade Estadual Paulista, Campus de Araraquara, Departamento de Bioprocessos e Biotecnologia, 14800903 Araraquara, São Paulo, Brazil.
authors.forename: Fernando Lucas
authors.initials: FL
authors.lastname: Primo
authors.affiliation: Institute of Physics of São Carlos, IFSC, University of São Paulo, USP, 13566590 São Carlos, SP, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Mafud
authors.affiliation: Institute of Physics of São Carlos, IFSC, University of São Paulo, USP, 13566590 São Carlos, SP, Brazil.
authors.forename: Larissa Fernandes
authors.initials: LF
authors.lastname: Batista
authors.affiliation: Institute of Physics of São Carlos, IFSC, University of São Paulo, USP, 13566590 São Carlos, SP, Brazil.
authors.forename: Yvonne Primerano
authors.initials: YP
authors.lastname: Mascarenhas
authors.affiliation: Nanomedicine and Nanotoxicology Group, Institute of Physics of São Carlos, IFSC, University of São Paulo, USP, 13566-590 São Carlos, SP, Brazil.
authors.forename: Iêda Maria Martinez
authors.initials: IMM
authors.lastname: Paino
authors.affiliation: Nanomedicine and Nanotoxicology Group, Institute of Physics of São Carlos, IFSC, University of São Paulo, USP, 13566-590 São Carlos, SP, Brazil.
authors.forename: Valtencir
authors.initials: V
authors.lastname: Zucolotto
authors.affiliation: Departamento de Química, Laboratório de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, USP, 14040901 Ribeirão Preto, SP, Brazil.
authors.forename: Antonio Claudio
authors.initials: AC
authors.lastname: Tedesco
authors.affiliation: Research Center in Biodiversity and Biotechnology, Biotec, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil.
authors.forename: Durcilene Alves
authors.initials: DA
authors.lastname: Silva
authors.affiliation: Research Center in Biodiversity and Biotechnology, Biotec, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí, Brazil; Área de Morfologia, Faculdade de Medicina, FM, Universidade de Brasília, UnB, Campus Universitário Darcy Ribeiro, Brasília, 70910900, Distrito Federal, Brazil.
authors.forename: José Roberto S A
authors.initials: JRSA
authors.lastname: Leite
authors.affiliation: Department of Chemistry, Campus Teresina, Federal University of Piauí, 64049-550 Teresina, Piauí, Brazil.
authors.forename: José Ribeiro
authors.initials: JR
authors.lastname: Dos Santos
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D001894:Borohydrides / Q000737:chemistry; D002351:Carrageenan / Q000633:toxicity; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D000067493:Dynamic Light Scattering; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D018625:Microscopy, Atomic Force; D010539:Permeability / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D001894:Borohydrides; D017382:Reactive Oxygen Species; D012834:Silver; C025364:sodium borohydride; D002351:Carrageenan; D003094:Collagen; D012835:Silver Nitrate
keywords: Collagen; L929 fibroblast cell; MV3 cancer cell; Nanoparticle; Nanotoxicology; Silver