title: Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry.
abstract: Lipids draw increasing attention of cell biologists because of the wide variety of functions beyond their role as building blocks of cellular membranes. Mitochondrial membranes possess characteristic lipid compositions that are intimately associated with mitochondrial architecture and activities. Therefore, quantitative assessment of lipids in isolated mitochondria is of importance for mitochondrial research. Here, I describe our workflow for quantitative analysis of glycerophospholipids in mitochondria with a focus on purification of pure mitochondrial fractions from yeast and cultured mammalian cells as well as improved settings for the analysis of cardiolipin by nano-electrospray ionization mass spectrometry.
authors.affiliation: Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Joseph-Stelzmann-Str. 26, 50674, Cologne, Germany. T.Tatsuta@uni-koeln.de.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tatsuta
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D020404:Glycerophospholipids / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D015003:Yeasts / Q000737:chemistry / Q000378:metabolism
chemical_list: D020404:Glycerophospholipids
keywords: Cardiolipin; Glycerophospholipids; Lipidomics; Mitochondria; Nano-electrospray ionization mass spectrometry