title: Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus × Epinephelus lanceolatus.
abstract: The hybrid grouper, Epinephelus fuscoguttatus (♀) × Epinephelus lanceolatus (♂), is a newly bred cultivated marine fish species of high economic value. However, a skin ulcer disease with high mortality has occurred, and the responsible pathogen remains unknown. In this study, we summarized the epidemic status and external signs of this disease. We screened potential pathogens and finally isolated one bacterial strain ML01 from affected fish. We subjected healthy juvenile hybrid groupers to bacterial challenge tests with the isolate by immersion, immersion after dermal abrasion and intraperitoneal injection, respectively. Within 14 days post-infection, the isolate ML01 caused mass mortality of juveniles infected via immersion after dermal abrasion or intraperitoneal injection. Diseased juveniles displayed obvious signs of skin ulcers. The median lethal dose of ML01 by intraperitoneal injection was 1.10 × 105colony-forming units. ML01 was identified as Vibrio harveyi by bacterial morphology, analytical profile index identification, 16S rDNA sequencing and multilocus sequence analysis. Antibiotic susceptibility tests showed that ML01 was sensitive to ceftriaxone, doxycycline and minocycline. The results of this study suggest that V. harveyi is the causal agent of skin ulcer disease in juvenile hybrid groupers, thus providing a basis for effective control and prevention of this disease.
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: G M
authors.initials: GM
authors.lastname: Shen
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: C Y
authors.identifier: http://orcid.org/0000-0001-7851-9273
authors.initials: CY
authors.lastname: Shi
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: C
authors.initials: C
authors.lastname: Fan
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: D
authors.initials: D
authors.lastname: Jia
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: G S
authors.initials: GS
authors.lastname: Xie
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Li
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Mo
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture of P. R. China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: J
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology*; D006824:Hybridization, Genetic; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012883:Skin Ulcer / Q000382:microbiology / Q000662:veterinary*; D014733:Vibrio / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*; D014774:Virulence
keywords: 
  Vibrio harveyi
  ; antibiotic susceptibility; grouper; identification; pathogenicity