title: Evaluation of anti-inflammatory effect of silver-coated glass beads in mice with experimentally induced colitis as a new type of treatment in inflammatory bowel disease.
abstract: BACKGROUND
  Recent studies point at the anti-inflammatory action of silver through induction of apoptosis of inflammatory cells via oxidative stress, promotion of wound healing as well as antimicrobial effect. Our aim was to design a new formulation based on silver and validate its anti-inflammatory activity in the mouse models of colitis.
  
  
  METHODS
  Silver-coated glass beads were prepared using a magnetron sputtering method and a standard magnetron sputtering gun equipped with pure silver target. Colitis was induced by the ic administration of TNBS into colon (to mimic Crohn's disease) and addition of DSS to drinking water (to imitate ulcerative colitis). Evaluation of inflammation was performed based on macroscopic and microscopic scoring, quantification of the myeloperoxidase activity and colonic microflora analysis.
  
  
  RESULTS
  Silver-coated glass beads administered ic alleviated intestinal inflammation in mouse models of colitis, induced by TNBS and DSS. This alleviation of colitis resulted principally from changes in the gut microflora. The anti-inflammatory action of the new formulation was associated predominantly with the presence of the silver nanolayer on the beads, and to a lesser extent the size of glass polymer units.
  
  
  CONCLUSIONS
  The application of the newly developed formulation employing silver-coated glass beads has the potential to be translated to clinical conditions for the efficient treatment of IBD.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland; Department of Vehicles and Fundamentals of Machine Design, Faculty of Mechanical Engineering, Lodz University of Technology, Łódź, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Siczek
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Zatorski
authors.affiliation: Department of Animal Hygiene and Environment, Faculty of Biology and Animal Breeding, University of Agriculture in Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Chmielowiec-Korzeniowska
authors.affiliation: Department of Pathology, Faculty of Medicine, Medical University of Lodz, Łódź, Poland.
authors.forename: Radzisław
authors.initials: R
authors.lastname: Kordek
authors.affiliation: Department of Animal Hygiene and Environment, Faculty of Biology and Animal Breeding, University of Agriculture in Lublin, Lublin, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Tymczyna
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Łódź, Poland. Electronic address: jakub.fichna@umed.lodz.pl.
authors.forename: Jakub
authors.initials: J
authors.lastname: Fichna
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003092:Colitis / Q000188:drug therapy / Q000473:pathology; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000473:pathology; D003424:Crohn Disease / Q000188:drug therapy* / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; D012834:Silver; D014302:Trinitrobenzenesulfonic Acid; D016264:Dextran Sulfate
keywords: Crohn’s disease; Inflammatory bowel disease; Microbiota; Silver-coated glass beads; Ulcerative colitis