title: The effect of kisspeptin on spermatogenesis and apoptosis in rats.
abstract: To study the effect of kisspeptin, a gonadotropin release stimulator, on the testicular tissue of the rat.
  
  
  
  Four groups were formed as follows: control, Kiss-10 501397645907nmol administration for 1 day, Kiss-10 administration for 13 days, and one last group kept for 7 days following Kiss-10 applied for 13 days. Testicular tissues were stained with hematoxylin-eosin, periodic acid Schiff, Masson trichrome staining, terminal deoxynucleotidyl transferased UTP nick-end labeling, and Ki-67 immune staining. Serum testosterone levels were determined.
  
  
  
  Serum testosterone level increased following acute application, while it was reduced by chronic treatment. Spermatogenic cells as stained by Ki-67 and TUNEL increased in the treated groups compared to the controls. Following a 7-day rest after treatment, a decrease in testosterone levels and Ki-67-stained cell numbers and an increase in TUNEL-stained cells were observed. Leydig cells showed increased vacuolization in the Kiss-1 group. Leydig cell vacuolization continued in the Kiss (13) group and was reduced in the Kiss (13 + 7) group.
  
  
  
  Kiss-10 increased spermatogenic cell proliferation, while testosterone level and proliferation decreased and apoptosis increased during the waiting period.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Medipol University, İstanbul, Turkey.
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Aytürk
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Turkey.
authors.forename: Tülin
authors.initials: T
authors.lastname: Firat
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Kükner
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors.forename: Candan
authors.initials: C
authors.lastname: Özoğul
authors.affiliation: Department of Physiology, Faculty of Medicine, SANKO University, Gaziantep, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Töre
authors.affiliation: Department of Urology Clinics, Bağcılar Education and Research Hospital, İstanbul, Turkey.
authors.forename: İsmail Engin
authors.initials: İE
authors.lastname: Kandirali
authors.affiliation: Department of Physiology, Faculty of Medicine, İstanbul Yeditepe University, İstanbul, Turkey.
authors.forename: Bayram
authors.initials: B
authors.lastname: Yilmaz
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D059648:Kisspeptins / Q000494:pharmacology*; D007985:Leydig Cells / Q000166:cytology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013091:Spermatogenesis / Q000187:drug effects*
chemical_list: D059648:Kisspeptins