title: Proteomic Biomarkers for Bisphenol A-Early Exposure and Women's Thyroid Cancer.
abstract: OBJECTIVE
  For the target treatment and prevention of women's increased thyroid cancer, we focused on risks of environmental exposure to endocrine disrupting chemicals, particularly bisphenol A (BPA), and its high susceptible exposure-timing, particularly early exposure in lives.
  
  
  METHODS
  Female ICR mice were exposed to BPAin uteroand in early life (15, 75, and 300 mg/L of drinking water via pregnant mice and lactation). We identified BPA-responsive proteins in mice thyroid by two-dimensional gel electrophoresis, image analyses, and electrospray ionization quadrupole time-of-flight mass spectrometry. We further analyzed expression of the BPA-responsive proteins in women thyroid cancer patients (n=28).
  
  
  RESULTS
  We found the altered 17 proteins in BPA dose-dependent manner among the thyroid tissues of offspring mice and identified nine proteins of them, including Anxa6, Atp5b, Hspa5, and Vcp, etc. In addition, we observed the positive association between blood BPA levels and mRNA expression of theANXA6andVCPnot in normal but thyroid cancer tissues.
  
  
  CONCLUSIONS
  Our study provides ANXA6 and VCP as proteomic biomarkers for BPA-early life exposure and their potential for women's thyroid cancer.
authors.affiliation: Department of Toxicology, Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul, Korea.
authors.forename: Ho-Sun
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Toxicology, Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul, Korea.
authors.forename: Yunkyeong
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Department of Otolaryngology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Kyung
authors.initials: K
authors.lastname: Tae
authors.affiliation: Department of Anatomy and Pathology, Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul, Korea.
authors.forename: Gyu-Un
authors.initials: GU
authors.lastname: Bae
authors.affiliation: Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.
authors.forename: Jong Y
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Biomedical and Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, University of Montana, Missoula, MT, USA.
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Department of Toxicology, Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul, Korea.
authors.forename: Mihi
authors.initials: M
authors.lastname: Yang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000009:adverse effects*; D015415:Biomarkers / Q000737:chemistry*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D010636:Phenols / Q000009:adverse effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D040901:Proteomics / Q000379:methods*; D013964:Thyroid Neoplasms / Q000235:genetics*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D015415:Biomarkers; D000091342:Endoplasmic Reticulum Chaperone BiP; D010636:Phenols; C000717247:HSPA5 protein, human; C000717248:Hspa5 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Annexin A6; Bisphenol A; Endocrine disruptors; Maternal exposure; Proteomics; Thyroid neoplasms; Valosin-containing protein; Women