title: Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
abstract: The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid β-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis.
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Sin Man
authors.initials: SM
authors.lastname: Lam
authors.affiliation: University of Chinese Academy of Sciences, Beijing, China.|Academy of Mathematics and Systems Science, National Center for Mathematics and Interdisciplinary Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jingxue
authors.initials: J
authors.lastname: Xin
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Academy of Mathematics and Systems Science, National Center for Mathematics and Interdisciplinary Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Guanghou
authors.initials: G
authors.lastname: Shui
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Xun
authors.identifier: 0000-0002-2653-8293
authors.initials: X
authors.lastname: Huang
mesh_terms: D000483:Alleles; D020816:Amino Acid Motifs; D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry*; D006706:Homeostasis; D008055:Lipids / Q000737:chemistry*; D009154:Mutation; D010100:Oxygen / Q000737:chemistry; D020675:Peroxisomes / Q000737:chemistry; D010641:Phenotype; D010743:Phospholipids / Q000737:chemistry; D034622:RNA Interference; D017423:Sequence Analysis, RNA; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D035341:Telomeric Repeat Binding Protein 2 / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D035381:TATA-Binding Protein Associated Factors; C509085:TRF2 protein, Drosophila; D035341:Telomeric Repeat Binding Protein 2; D035362:Transcription Factor TFIID; C084120:e(y)1 protein, Drosophila; D010100:Oxygen