title: Expression Pattern of ERF Gene Family under Multiple Abiotic Stresses inPopulus simonii × P. nigra.
abstract: Identification of gene expression patterns of key genes across multiple abiotic stresses is critical for mechanistic understanding of stress resistance in plant. In the present study, we identified differentially expressed genes (DEGs) in di-haploidPopulus simonii × P. nigraunder respective stresses of NaCl, KCl, CdCl2,and PEG. On the basis of RNA-Seq, we detected 247 DEGs that are shared by the four stresses in wild type poplar, and mRNA abundance of the DEGs were validated in transgenic poplar overexpressingERF76gene by RNA-Seq and RT-qPCR. Results from gene ontology analysis indicated that these genes are enriched in significant pathways, such as phenylpropanoid biosynthesis, phenylalanine metabolism, starch and sucrose metabolism, and plant hormone signal transduction. Ethylene response factor (ERF) gene family plays significant role in plant abiotic stress responses. We also investigated expression pattern of ERF gene family under the four stresses. The ERFs and DEGs share similar expression pattern across the four stresses. The transgenic poplar is superior to WT in morphologic, physiological and biochemical traits, which demonstrated theERF76gene plays a significant role in stress resistance. These studies will give a rise in understanding the stress response mechanisms in poplar.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityHarbin, China; Northeast Institute of Geography and Agroecology, Chinese Academy of SciencesHarbin, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Boru
authors.initials: B
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Renhua
authors.initials: R
authors.lastname: Li
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Tingbo
authors.initials: T
authors.lastname: Jiang
keywords: ERF transcription factor; ERF76 gene; abiotic stresses; expression pattern; poplar