title: Effects of octylphenol on the expression of StAR, CYP17 and CYP19 in testis of Rana chensinensis.
abstract: It has been proposed that a decline in sperm quality is associated with exposure to environmental chemicals with estrogenic activity. Seeking possible explanations for this effect, this study investigated the effects of octylphenol (OP) on the synthesis of steroid hormones in amphibian. Rana chensinensis were exposed to 10-8, 10-7and 10-6mol/L OP after 10, 20, 30 and 40 days. The cDNA fragments of StAR (274bp), CYP17 (303bp) and CYP19 (322bp) were cloned. In situ hybridization and immunohistochemistry revealed that positive signals of StAR, CYP17, CYP19 mRNA and proteins mainly in the Leydig cells of testes. Real-time PCR showed that up-regulation of StAR and CYP19, and down-regulation of CYP17 after exposure to 10-8, 10-7and 10-6mol/L OP. The results suggest that OP can alter transcriptions of StAR, CYP17 and CYP19, thus disturb the expressions of StAR, P450c17 and P450arom, thereby adversely affect steroid synthesis.
authors.affiliation: College of Life Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, China; China National Center for Food Safety Risk Assessment, 7 Pan Jia Yuan Nan Li, Beijing 100021, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Bai
authors.affiliation: College of Life Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, China.
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Li
authors.affiliation: Second Division of Beijing Armed Police Corps, Beijing 100076, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Liu
authors.affiliation: College of Life Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, China. Electronic address: yu-huizhang@163.com.
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics*; D052244:Endocrine Disruptors / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D010636:Phenols / Q000633:toxicity*; D010750:Phosphoproteins / Q000235:genetics*; D011898:Ranidae; D013091:Spermatogenesis / Q000187:drug effects / Q000235:genetics; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D052244:Endocrine Disruptors; D010636:Phenols; D010750:Phosphoproteins; C474055:octylphenol; D000098682:Steroidogenic Acute Regulatory Protein; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase
keywords: CYP17; CYP19; Octylphenol; Rana chensinensis; StAR