title: The Osteogenic Potential of Human Nondifferentiated and Pre-differentiated Mesenchymal Stem Cells Combined with an Osteoconductive Scaffold - Early Stage Healing.
abstract: Despite the huge research into stem cells and their regenerative properties for bone healing, there are still unanswered questions including the recipient's respond to the presence of the stem cells, the fate of stem cells inside the bone defect and the possible advantage in utilizing pre-differentiated cells. To address these problems, we used human multipotent mesenchymal stromal/stem cells (MSCs), GMP Grade, in a rat model of bone formation. In a "bioreactor concept" approach seven Wistar rats were implanted with 0.2 g of synthetic bone scaffold seeded with 2 × 106 MSCs, seven Wistar rats were implanted with 0.2 g of synthetic bone scaffold seeded with 1 × 106 predifferentiated osteoblasts and 1 × 106 pre-differentiated endothelial cells and 14 Wistar rats were implanted with 0.2 g of synthetic bone scaffold without seeded cells into an intramuscular pocket on the left side of their back. The right side of each rat was used as a control, and 0.2 g of synthetic bone scaffold was implanted into the intramuscular pocket alone. To see the early stage healing the samples were harvested 14 days after the implantation, MSCs were detected by positive DAPI and MTCO2 staining in 43% of all the samples implanted with MSCs, and no inflammation signs were present in any implanted animal. New vessels could be found in both groups implanted with MSCs, but not in the control group of animals. However, hematoxylin-eosin staining could not detect newly created bone within the implant in any of the groups. These results were in line with COLL1 staining, where we could detect positive staining only in three cases, all of which were implanted with un-differentiated MSCs. According to our findings, there were no benefits of using the pre-differentiated of MSC.
authors.affiliation: Department of Dentistry, Charles University, Medical Faculty and University Hospital Hradec Králové, Czech Republic.
authors.forename: Luboš
authors.initials: L
authors.lastname: Tuček
authors.affiliation: Bioinova, Ltd, Prague, Czech Republic.|Department of Neuroscience, 2nd Faculty of Medicine, Charles University, Czech Republic.|Institute of Experimental Medicine ASCR, Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kočí
authors.affiliation: Department of Neuroscience, 2nd Faculty of Medicine, Charles University, Czech Republic.|Institute of Experimental Medicine ASCR, Prague, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Kárová
authors.affiliation: Department of Dentistry, Charles University, Medical Faculty and University Hospital Hradec Králové, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Doležalová
authors.affiliation: Department of Dentistry, Charles University, Medical Faculty and University Hospital Hradec Králové, Czech Republic. suchanekj@lfhk.cuni.cz.
authors.forename: Jakub
authors.initials: J
authors.lastname: Suchánek
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D018919:Neovascularization, Physiologic; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D017208:Rats, Wistar
keywords: bone defect; mesenchymal stromal/stem cells; osteoconduction; osteoinduction; scaffold; tissue bone engineering