title: Iwr1 facilitates RNA polymerase II dynamics during transcription elongation.
abstract: Iwr1 is an RNA polymerase II (RNPII) interacting protein that directs nuclear import of the enzyme which has been previously assembled in the cytoplasm. Here we present genetic and molecular evidence that links Iwr1 with transcription. Our results indicate that Iwr1 interacts with RNPII during elongation and is involved in the disassembly of the enzyme from chromatin. This function is especially important in resolving problems posed by damage-arrested RNPII, as shown by the sensitivity of iwr1 mutants to genotoxic drugs and the Iwr1's genetic interactions with RNPII degradation pathway mutants. Moreover, absence of Iwr1 causes genome instability that is enhanced by defects in the DNA repair machinery.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, Burjassot, Valencia 46100, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Gómez-Navarro
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, Burjassot, Valencia 46100, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Peiró-Chova
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, Burjassot, Valencia 46100, Spain. Electronic address: estruch@uv.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Estruch
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D042822:Genomic Instability / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics* / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D002843:Chromatin; C544940:IWR1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II
keywords: Genome instability; Iwr1; RNA polymerase; Transcription coupled repair; Transcription elongation