title: Chemical constituents and in vitro biological activities of Eremurus spectabilis leaves.
abstract: In the present study, phytochemical investigation of the young leaves of Eremurus spectabilis let to the isolation six compounds (1-6). Their structure was determined by spectroscopic analysis and comparison with the literature data. All compounds were isolated for the first time from E. spectabilis. Isolated compounds and prepared extracts did not show cytotoxic activity against HeLa (Human cervix adenocarcinoma), A-549 (Human alveolar adenocarcinoma), MCF-7 (Human breast adenocarcinoma), mPANC96 (Human pancreas adenocarcinoma), U87MG (Glioblastoma), PC3 (Prostate), CaCo-2 (Colon), HEK293 (Human embryonic kidney cells) cell lines. RAW 264.7 (murine macrophage cell lines) was used to determine the inhibition levels of inducible nitric oxide synthase (iNOS).The hexane extract was found to be active in terms of inhibition of iNOS (IC50: 25 μg/ml).
authors.affiliation: a Faculty of Science, Department of Chemistry , Ege University , Bornova-Izmir , Turkey.
authors.forename: Leyla
authors.initials: L
authors.lastname: Karakaya
authors.affiliation: a Faculty of Science, Department of Chemistry , Ege University , Bornova-Izmir , Turkey.
authors.forename: Yurdanur
authors.initials: Y
authors.lastname: Akgül
authors.affiliation: b Faculty of Engineering, Department of Bioengineering , Ege University , Bornova-Izmir , Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Nalbantsoy
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005260:Female; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D051379:Mice; D015394:Molecular Structure; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
keywords: Cytotoxicity and iNOS activities; Eremurus spectabilis; Isolated compounds