title: miR-9 regulates the multidrug resistance of chronic myelogenous leukemia by targeting ABCB1.
abstract: Multidrug resistance (MDR) is commonly correlated with the poor prognosis of chronic myelogenous leukemia. Aberrant expression of microRNAs (miRNAs) plays an important role in the MDR of various types of cancers. MicroRNA-9 (miR-9) has been confirmed to be dysregulated in many types of cancers. However, the relationship between miR-9 and MDR in chronic myelogenous leukemia (CML) remains largely unknown. In the present study, we showed that miR‑9-3p (miR-9) was downregulated in adriamycin (ADR)‑resistant K562/ADR cells and CML/MDR patients. Overexpression of miR-9 was sufficient to reverse cancer cell resistance to multiple chemotherapeutics in vitro and sensitized tumors to chemotherapy in vivo. Furthermore, we found that ABCB1 is a direct target of miR-9. These findings suggest that miR-9 plays a critical role in MDR in CML by targeting ABCB1. These results provide new evidence of miR-9 as a biomarker for clinical diagnosis and as a potential therapeutic target for CML.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Nana
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D015536:Down-Regulation; D004317:Doxorubicin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D055815:Young Adult
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D004317:Doxorubicin