title: Microchannels for the Study of T Cell Immunological Synapses and Kinapses.
abstract: T Cells can form very stable (synapses) or very transient and migratory (kinapses) contacts with antigen-presenting cells. Here, we describe how microchannels can be used to conveniently study the distinct dynamics of T cells during antigen recognition. Microchannels provide a controlled confined environment that promotes T cell migration and recapitulates kinapse and synapse behaviors when coated with appropriate pMHC molecules. We also depict the advantages of this in vitro approach for addressing mechanistic issues and for analysis.
authors.affiliation: INSERM U932, Institut Curie, PSL Research University, 75005, Paris, France.|Dynamics of Immune Responses Unit, Institut Pasteur, 75015, Paris, France.|INSERM U1223, 75015, Paris, France.
authors.forename: Hélène D
authors.initials: HD
authors.lastname: Moreau
authors.affiliation: Dynamics of Immune Responses Unit, Institut Pasteur, 75015, Paris, France.|INSERM U1223, 75015, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bousso
authors.affiliation: INSERM U932, Institut Curie, PSL Research University, 75005, Paris, France. Ana-Maria.Lennon@curie.fr.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Lennon-Duménil
mesh_terms: D002465:Cell Movement / Q000276:immunology*; D006801:Humans; D054992:Immunological Synapses / Q000276:immunology*; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology*
keywords: Antigen affinity; Imaging; Microchannels; Recombinant pMHC; Synapse/kinapse