title: Investigation of deactivation thermodynamics of lipase immobilized on polymeric carrier.
abstract: In the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. Immobilization of lipase Candida rugosa (CRL) was carried out on biocompatible blend of poly vinyl alcohol (PVA) and chitosan (CHY) support via entrapment and glutardehyde (Glu) cross-linking method to produce PVA:CHY:CRL and PVA:CHY:Glu:CRL as robust biocatalyst. These immobilized lipases were characterized by various physico-biochemical characterization techniques. Later on, thermal and solvent stability of polymer immobilized lipase was determined in term of half-life time (t0.5), D values, enthalpy (ΔH°), entropy (ΔS°), and free energy (ΔG°) of deactivation at different temperatures and in various solvents. The thermodynamic deactivation stability trend was found as: cross-linked lipase CRL > entrapped lipase CRL > free lipase CRL. Moreover, kinetic parameters, such as Km, Vmax,and catalytic efficiency, were also determined to understand the kinetic features. The polymer immobilized enzyme was reused to investigate the economic viability of the developed biocatalyst.
authors.affiliation: Department of Chemistry, Institute of Chemical Technology, Mumbai, 400019, India.
authors.forename: Kirtikumar C
authors.initials: KC
authors.lastname: Badgujar
authors.affiliation: Department of Chemistry, Institute of Chemical Technology, Mumbai, 400019, India. bm.bhanage@gmail.com.
authors.forename: Bhalchandra M
authors.initials: BM
authors.lastname: Bhanage
mesh_terms: D002175:Candida / Q000201:enzymology*; D048271:Chitosan / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D008049:Lipase / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011142:Polyvinyl Alcohol; D048271:Chitosan; D008049:Lipase; D005976:Glutaral
keywords: Deactivation study; Enzyme activity; Immobilized enzymes; Lipase; Thermodinamics