title: Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection.
abstract: Sensitive detection of gizzerosine, a causative agent for deadly gizzard erosion in chicken feeds, is very important to the poultry industry. In this work, a new method was developed based on microchip capillary electrophoresis (MCE) with laser-induced fluorescence (LIF) detection for rapid analysis of gizzerosine, a biogenic amine in fish meals. The MCE separation was performed on a glass microchip using sodium dodecyl sulfate (SDS) as dynamic coating modifier. Separation conditions, including running buffer pH and concentration, SDS concentration, and the separation voltage were investigated to achieve fast and sensitive quantification of gizzerosine. The assay proposed was very quick and could be completed within 65 s. A linear calibration curve was obtained in the range from 0.04 to 1.8 μg ml-1gizzerosine. The detection limit was 0.025 μg ml-1(0.025 mg kg-1), which was far more sensitive than those previously reported. Gizzerosine was well separated from other endogenous components in fish meal samples. Recovery of gizzerosine from this sample matrix (n = 3) was determined to be 97.2-102.8%. The results from analysing fish meal samples indicated that the present MCE-LIF method might hold the potential for rapid detection of gizzerosine in poultry feeds.
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.|b University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Meng-Wei
authors.initials: MW
authors.lastname: Xiao
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Bai
authors.affiliation: c The Ecological Technique and Engineering College , Shanghai Institute of Technology , Shanghai , China.
authors.forename: Pei-Li
authors.initials: PL
authors.lastname: Xu
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.|b University of Chinese Academy of Sciences , Beijing , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: d Beijing Institute of Genomics , Chinese Academy of Sciences , Beijing , China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.|e Department of Chemistry and Biochemistry , Jackson State University , Jackson , MS , USA.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.
authors.forename: Xun
authors.initials: X
authors.lastname: Liao
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D046209:Electrophoresis, Microchip; D005399:Fishes; D005453:Fluorescence; D007093:Imidazoles / Q000032:analysis*; D007834:Lasers
chemical_list: D007093:Imidazoles; C045114:gizzerosine
keywords: Microchip capillary electrophoresis; gizzerosine; laser-induced fluorescence; poultry feeds; surface coating