title: [The effect of FII c.1787G>A (prothrombin Belgrade) mutation on prothrombin gene expression in vitro].
abstract: The FII c.1787G>A (prothrombin Belgrade) is a novel prothrombotic mutation which leads to impaired inhibition of thrombin by antithrombin (antithrombin resistance). So far, the mechanism of this variant has not been fully elucidated. To investigate the effect of FII c.1787G>A mutation on the prothrombin gene expression, its functional analysis was performed in vitro. By Real-Time PCR, expression levels of FII gene variants were evaluated in Cos-7 cells transiently transfected with c.1787G (wild-type) and c.1787A prothrombin expression vectors, with no differences observed. The relative quantification of prothrombin protein amounts was accomplished by Western blot analysis, also with no differences observed. Therefore, the mechanism of FII c.1787G>A mutation does not alter prothrombin expression profile.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia.|majagvozdenov@yahoo.com.
authors.forename: M
authors.initials: M
authors.lastname: Gvozdenov
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia.
authors.forename: I
authors.initials: I
authors.lastname: Pruner
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia.
authors.forename: B
authors.initials: B
authors.lastname: Tomic
authors.affiliation: Faculty of Medicine, University of Belgrade, Serbia.|Blood Transfusion Institute of Serbia, Hemostasis Department, Belgrade, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Kovac
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia.
authors.forename: D
authors.initials: D
authors.lastname: Radojkovic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia.
authors.forename: V
authors.initials: V
authors.lastname: Djordjevic
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D015870:Gene Expression; D009154:Mutation; D011516:Prothrombin / Q000235:genetics*; D013917:Thrombin
chemical_list: D011516:Prothrombin; D013917:Thrombin
keywords: FII c.1787G>A mutation; prothrombin Belgrade; prothrombin gene expression