title: Correlation between Trop2 and amphiregulin coexpression and overall survival in gastric cancer.
abstract: Gastric cancer (GC) is a multistep and multistage disease and the majority of GC cells could overexpressed one or more oncogenes. Trop2 and amphiregulin (AREG) are both overexpressed in various epithelial cell cancers and have the role in the increases tumor cells division and metastasis. However, little is known about the function and correlation of two oncogenes coexpressed in GC. The expression level of these two genes in 791 cases of GC tissues were tested, the correlations between two genes expression and clinical pathological characteristics and overall survival in GC patients through immunohistochemistry (IHC) were analyzed. This study also explored the mRNA expression level of two genes in 26 cases of freshly GC tissues by qRT-PCR. The results indicated that Trop2+/AREG+ coexpression was higher in GC tissues than in adjacent tissues. Trop2+/AREG+ protein coexpression were associated with Tumor Node Metastasis (TNM) stage (χ2= 50.345, P < 0.001), tumor size (χ2= 40.349, P < 0.001), lymph node metastases (χ2= 26.481, P < 0.001), and distant metastases (χ2= 8.387, P = 0.039). GC patients with Trop2+ and AREG+ protein coexpression had poor overall survival rates (HR = 3.682, 95% CI = 2.038-6.654, P < 0.001). The expression level of Trop2/AREG were positively correlated (r 0.254 and P < 0.001). The result of the mRNA expression was similar to that of the protein expression. Overall, Trop2 and AREG could be seen as prognostic cobiomarker in GC and combined detection of Trop2 and AREG could be viewed as helpful in predicting the prognosis of the GC patients.
authors.affiliation: Department of Pathology, Nanjing Medical University, Nanjing, 210029, China.|School of Public Health, Nantong University, Nantong, 226019, China.
authors.forename: Wei
authors.identifier: http://orcid.org/0000-0001-8557-297X
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Guipeng
authors.initials: G
authors.lastname: Ding
authors.affiliation: Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Wen
authors.affiliation: Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Oncology, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an, 223001, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yong
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, 226019, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, 226019, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of Pathology, Nanjing Medical University, Nanjing, 210029, China.|Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, 210029, China.|Jiangsu Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, 210029, China.|Key Laboratory of Cancer Biomarkers, Prevent and Treatment, Cancer Center, Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, 210029, China.|Huadong Medical Institute of Biotechniques, Nanjing, 210029, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D066258:Amphiregulin / Q000235:genetics* / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016019:Survival Analysis; D015854:Up-Regulation
chemical_list: C587848:AREG protein, human; D066258:Amphiregulin; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499380:TACSTD2 protein, human
keywords: 
  
  AREG
  
  ; 
  Trop2
  ; 
  coexpression
  ; 
  correlation
  ; 
  gastric cancer
  