title: Humoral Responses to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR Regulon-Encoded Proteins ofMycobacterium tuberculosisin Individuals with Latent Tuberculosis Infection.
abstract: Latent tuberculosis infection (LTBI) is evidence of immunological control of tuberculosis. Dormancy survival regulator (DosR) regulon-encoded proteins may have a role in the maintenance of LTBI. T cell responses to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR regulon-encoded proteins were found to be most frequent among household contacts of TB cases from Uganda compared to other DosR proteins, but antibody responses were not described. We characterized antibody responses to these proteins in individuals from Uganda. Antibodies to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR regulon-encoded proteins were measured in 68 uninfected individuals, 62 with LTBI, and 107 with active pulmonary tuberculosis (APTB) cases. There were no differences in the concentrations of antibodies to Rv0081, Rv1735c, and Rv1737c DosR regulon-encoded proteins between individuals with LTBI and APTB and those who were uninfected. LTBI was associated with higher concentrations of antibodies to Rv1733c in female participants [adjusted geometric mean ratio: 1.812, 95% confidence interval (CI): 1.105 2.973, andp= 0.019] but not in males (pvalue for interaction = 0.060). Antibodies to the four DosR regulon-encoded proteins investigated may not serve as good biomarkers of LTBI in the general population. More of theM.tbproteome needs to be screened to identify proteins that induce strong antibody responses in LTBI.
authors.affiliation: College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda; Medical Research Council/UVRI Uganda Research Unit on AIDS, P.O. Box 49, Plot 51-59 Nakiwogo Road, Entebbe, Uganda.
authors.forename: Simon G
authors.identifier: 0000-0001-6818-617X
authors.initials: SG
authors.lastname: Kimuda
authors.affiliation: Medical Research Council/UVRI Uganda Research Unit on AIDS, P.O. Box 49, Plot 51-59 Nakiwogo Road, Entebbe, Uganda; Department of Clinical Research, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Angela
authors.initials: A
authors.lastname: Nalwoga
authors.affiliation: Medical Research Council/UVRI Uganda Research Unit on AIDS, P.O. Box 49, Plot 51-59 Nakiwogo Road, Entebbe, Uganda.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Levin
authors.affiliation: Department of Immunohematology and Blood Transfusion and Department of Infectious Diseases, Leiden University Medical Centre, 2300 RC Leiden, Netherlands.
authors.forename: Kees L M C
authors.identifier: 0000-0002-4747-6060
authors.initials: KL
authors.lastname: Franken
authors.affiliation: Department of Immunohematology and Blood Transfusion and Department of Infectious Diseases, Leiden University Medical Centre, 2300 RC Leiden, Netherlands.
authors.forename: Tom H M
authors.initials: TH
authors.lastname: Ottenhoff
authors.affiliation: Medical Research Council/UVRI Uganda Research Unit on AIDS, P.O. Box 49, Plot 51-59 Nakiwogo Road, Entebbe, Uganda; Department of Clinical Research, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Alison M
authors.initials: AM
authors.lastname: Elliott
authors.affiliation: College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda; Medical Research Council/UVRI Uganda Research Unit on AIDS, P.O. Box 49, Plot 51-59 Nakiwogo Road, Entebbe, Uganda; Department of Clinical Research, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Stephen
authors.initials: S
authors.lastname: Cose
authors.affiliation: College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda; Medical Research Council/UVRI Uganda Research Unit on AIDS, P.O. Box 49, Plot 51-59 Nakiwogo Road, Entebbe, Uganda.
authors.forename: Irene
authors.initials: I
authors.lastname: Andia-Biraro
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood; D055985:Latent Tuberculosis / Q000276:immunology* / Q000382:microbiology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011494:Protein Kinases / Q000235:genetics*; D018085:Regulon; D013601:T-Lymphocytes / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C542863:DosR protein, Mycobacterium tuberculosis; D007074:Immunoglobulin G; C000589489:Rv1733c protein, Mycobacterium tuberculosis; D011494:Protein Kinases