title: Evaluation of candida albicans biofilm formation on various dental restorative material surfaces.
abstract: Candida adhesion to any oral substrata is the first and essential stage in forming a pathogenic fungal biofilm. In general, yeast cells have remarkable potential to adhere to host surfaces, such as teeth or mucosa, and to artificial, nonbiological surfaces, such as restorative dental materials. This study compared the susceptibility of six dental restorative materials to Candida albicans adhesion.
  
  
  
  Cylindrical samples of each material were made according to the manufacturersa instructions. The antifungal effect of the samples on C. albicans was determined with the disc-diffusion method. The samples were put in plates with sterile Mueller Hinton and Sabouraud dextrose agar previously seeded with C. albicans. After the incubation period, the inhibition zone around each sample was evaluated. To evaluate the biofilm formation, the XTT technique and scanning electron microscopy (SEM) were used.
  
  
  
  No inhibition zone was observed around the samples. According to the XTT assays, the amalgam samples revealed the lowest quantity of biofilm formation (P > 0.001). The highest median XTT values, significantly higher than the other materials (P < 0.001), were found for the composite and the compomer samples. Within the SEM examination, the amount of candidal growth was significantly lower on the resin-modified glass ionomer and glass-ionomer cement samples. The compomer and the composite samples showed more candidal adhesion.
  
  
  
  This finding emphasizes the use of glass ionomer restorative cements and amalgam to reduce C. albicans adhesion to dental restorative materials especially in people with weakened immune systems, neutropenia, and cancer.
authors.affiliation: Department of Paediatric Dentistry, Faculty of Dentistry, Ordu University, Ordu, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Beldüz
authors.affiliation: Department of Microbiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Kamburoğlu
authors.affiliation: Paediatric Dentistry, Private Dentist, Bursa, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Yılmaz
authors.affiliation: Department of Microbiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Tosun
authors.affiliation: Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Beldüz
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Ordu University, Ordu, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Kara
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology*; D020770:Compomers / Q000494:pharmacology; D003188:Composite Resins / Q000494:pharmacology; D003723:Dental Amalgam / Q000494:pharmacology; D003764:Dental Materials / Q000494:pharmacology*; D003793:Dental Restoration, Permanent / Q000295:instrumentation; D052978:Disk Diffusion Antimicrobial Tests; D005899:Glass Ionomer Cements / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties
chemical_list: D020770:Compomers; D003188:Composite Resins; D003764:Dental Materials; D005899:Glass Ionomer Cements; D003723:Dental Amalgam