title: Monitoring of the functional properties and unfolding change of Ovalbumin after DHPM treatment by HDX and FTICR MS: Functionality and unfolding of Oval after DHPM by HDX and FTICR MS.
abstract: Dynamic high-pressure microfluidizaiton (DHPM) induced unfolding of proteins and enzymes could enhance the functional properties and hydrolytic ability of the molecules. In this study, the effect of DHPM on the surface activities of Ovalbumin (Oval), namely foaming and emulsifying properties, was investigated at 0, 40, 80, 120 and 160MPa. Improvement in the foaming and emulsifying properties was observed at 120 and 80MPa, respectively. Hydrogen/deuterium exchange (HDX) coupled with Fourier transform infrared spectroscopy-mass spectrometry (FTICR MS) was performed to investigate the exact unfolded region of Oval and analyze the mechanism promoting the foaming and emulsifying properties after 120 and 80MPa, respectively, compared with those at 0MPa. Prevalent unfolding of Oval after 80 and 120MPa treatment was revealed by HDX-MS, indicating a more flexible conformation induced by DHPM. However, lower deuterium incorporation was observed for the large six-stranded β-sheet. Therefore, the combined forces could enhance the flexibility and surface activities of Oval, which increased its foaming and emulsifying properties.
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
authors.forename: Guang-Xian
authors.initials: GX
authors.lastname: Liu
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: tuzc_mail@aliyun.com.
authors.forename: Zong-Cai
authors.initials: ZC
authors.lastname: Tu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: wanghui00072@aliyun.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Da
authors.initials: D
authors.lastname: Ma
mesh_terms: D003903:Deuterium / Q000737:chemistry; D041961:Deuterium Exchange Measurement; D006859:Hydrogen / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D010047:Ovalbumin / Q000737:chemistry*; D011312:Pressure; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D006859:Hydrogen; D010047:Ovalbumin; D003903:Deuterium
keywords: 3D structure; DHPM; HDX FTICR MS; Ovalbumin; Unfolding