title: Detection and Genetic Environment of Pleuromutilin-Lincosamide-Streptogramin A Resistance Genes in Staphylococci Isolated from Pets.
abstract: Increasing emergence of staphylococci resistant to pleuromutilins, lincosamides, and streptogramin A (PLSA) and isolated from humans and pets is a growing public health concern worldwide. Currently, there was only one published study regarding one of the PLSAgenes,vga(A) detected in staphylococci isolated from cat. In this study, eleven pleuromutilin-resistant staphylococci from pets and two from their owners were isolated and further characterized for their antimicrobial susceptibilities, plasmid profiles, genotypes, and genetic context of the PLSAresistance genes. The genesal(A) identified in 11 staphylococcal isolates was found for the first time inStaphylococcus haemolyticus, Staphylococcus epidermidis, andStaphylococcus xylosus. Moreover, these 11 isolates shared the identical regions flanking thesal(A) gene located in the chromosomal DNA. TwoS. haemolyticusisolates from a cat and its owner carried similarvga(A)LCplasmids and displayed indistinguishable PFGE patterns. A novel chromosomal multidrug resistance genomic island (MDRGI) containing 13 resistance genes, includinglsa(E), was firstly identified inS. epidermidis. In addition,vga(A)LC,sal(A), andlsa(E) were for the first time identified in staphylococcal isolates originating from pet animals. The plasmids, chromosomal DNA region, and MDRGI associated with the PLSAresistance genesvga(A),vga(A)LC,sal(A), andlsa(E) are present in staphylococci isolated from pets and humans and present significant challenges for the clinical management of infections by limiting therapeutic options.
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural UniversityBeijing, China; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangzhou, China.
authors.forename: Fengru
authors.initials: F
authors.lastname: Deng
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University Beijing, China.
authors.forename: Huiwen
authors.initials: H
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University Beijing, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin Berlin, Germany.
authors.forename: Andrea T
authors.initials: AT
authors.lastname: Feßler
authors.affiliation: Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin Berlin, Germany.
authors.forename: Geovana B
authors.initials: GB
authors.lastname: Michael
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural UniversityBeijing, China; Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität BerlinBerlin, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
keywords: PLSA genes; human; multidrug resistance; pet; staphylococci