title: Dose- and time- effect responses of DNA methylation and histone H3K9 acetylation changes induced by traffic-related air pollution.
abstract: As an important risk factor of respiratory disorders, traffic-related air pollution (TRAP) has caused extensive concerns. Epigenetic change has been considered a link between TRAP and respiratory diseases. However, the exact effects of TRAP on epigenetic changes are still unclear. Here we investigated the dose- and time- effect responses of TRAP on DNA methylations and H3K9 acetylation (H3K9ac) in both blood and lung tissues of rats. The findings showed that every 1 μg/m3increase of TRAP components were associated with changes in %5 mC (95% CI) in LINE-1, iNOS, p16CDKN2A, and APC ranging from -0.088% (-0.150, -0.026) to 0.102 (0.049, 0.154), as well as 0.276 (0.053, 0.498) to 0.475 (0.103, 0.848) ng/mg increase of H3K9ac. In addition, every 1 more day exposure at high level of TRAP (in tunnel) also significantly changed the levels of DNA methylation (ranging from -0.842% to 0.248%) and H3K9ac (16.033 and 15.718 ng/mg pro in PBMC and lung tissue, respectively) changes. Season and/or sex could interact with air pollutants in affecting DNA methylation and H3K9ac. The findings showed that TRAP exposure is dose- and time- dependently associated with the changes of DNA methylation and H3K9ac.
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.|Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ding
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yongtang
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xinneng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Huaizhuang
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Cardiothoracic Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Environmental Epigenetics Laboratory, Department of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Institute of Pharmacology, Zhejiang University School of Pharmacology, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yinchun
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000107:Acetylation; D000393:Air Pollutants / Q000009:adverse effects; D000397:Air Pollution / Q000009:adverse effects*; D019175:DNA Methylation; D004781:Environmental Exposure / Q000009:adverse effects; D044127:Epigenesis, Genetic; D005260:Female; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008297:Male; D052638:Particulate Matter; D012621:Seasons; D012737:Sex Factors; D013997:Time Factors; D001335:Vehicle Emissions
chemical_list: D000393:Air Pollutants; D006657:Histones; D052638:Particulate Matter; D001335:Vehicle Emissions