title: Early serum biomarker networks in infants with distinct retinochoroidal lesion status of congenital toxoplasmosis.
abstract: The present study characterized the early changes in the serum chemokines/cytokine signatures and networks in infants with congenital-toxoplasmosis/(TOXO) as compared to non-infected-controls/(NI). TOXO were subgrouped according to the retinochoroidal lesion status as no-lesion/(NL), active-lesion/(ARL), active/cicatricial-lesion/(ACRL) and cicatricial-lesion/(CRL). The results showed that TOXO display prominent chemokine production mediated by IL-8/CXCL8, MIG/CXCL9, IP-10/CXCL10 and RANTES/CCL5. Additionally, TOXO is accompanied by mixed proinflammatory/regulatory cytokine pattern mediated by IL-6, IFN-γ, IL-4, IL-5 and IL-10. While TNF appears as a putative biomarker for NL and IFN-γ/IL-5 as immunological features for ARL, IL-10 emerges as a relevant mediator in ACRL/CRL. IL-8/CXCL8 and IP-10/CXCL10 are broad-spectrum indicators of ocular disease, whereas TNF is a NL biomarker, IFN-γ and MIG/CXCL9 point out to ARL; and IL-10 is highlighted as a genuine serum biomarker of ACRL/CRL. The network analysis demonstrated a broad chemokine/cytokine crosstalk with divergences in the molecular signatures in patients with different ocular lesions during congenital toxoplasmosis.
authors.affiliation: Universidade Federal de Uberlândia, Avenida João Naves de Ávila 2121, Santa Mônica, 38408-100 Uberlândia, MG, Brazil; Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Thádia Evelyn
authors.initials: TE
authors.lastname: de Araújo
authors.affiliation: Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Jordana Grazziela
authors.initials: JG
authors.lastname: Coelho-Dos-Reis
authors.affiliation: Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Samantha Ribeiro
authors.initials: SR
authors.lastname: Béla
authors.affiliation: Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Ana Carolina Aguiar Vasconcelos
authors.initials: ACAV
authors.lastname: Carneiro
authors.affiliation: Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Anderson Silva
authors.initials: AS
authors.lastname: Machado
authors.affiliation: Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Ludmila Melo
authors.initials: LM
authors.lastname: Cardoso
authors.affiliation: Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Ágata Lopes
authors.initials: ÁL
authors.lastname: Ribeiro
authors.affiliation: Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Michelle Hallais França
authors.initials: MHF
authors.lastname: Dias
authors.affiliation: Departamento de Pediatria, Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, Santa Efigênia, 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: Gláucia Manzan
authors.initials: GM
authors.lastname: Queiroz Andrade
authors.affiliation: Departamento de Oftalmologia e Otorrinolaringologia, Faculdade de Medicina da UFMG, Belo Horizonte, MG, Brazil.
authors.forename: Daniel Vitor
authors.initials: DV
authors.lastname: Vasconcelos-Santos
authors.affiliation: Núcleo de Ações e Pesquisa em Apoio Diagnóstico (NUPAD), Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, Santa Efigênia, 30130-100 Belo Horizonte, MG, Brazil.
authors.forename: José Nélio
authors.initials: JN
authors.lastname: Januário
authors.affiliation: Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.affiliation: Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Ricardo Wagner Almeida
authors.initials: RWA
authors.lastname: Vitor
authors.affiliation: Universidade Federal de Uberlândia, Avenida João Naves de Ávila 2121, Santa Mônica, 38408-100 Uberlândia, MG, Brazil.
authors.forename: Eloisa Amália Vieira
authors.initials: EAV
authors.lastname: Ferro
authors.affiliation: Universidade Federal de Uberlândia, Avenida João Naves de Ávila 2121, Santa Mônica, 38408-100 Uberlândia, MG, Brazil; Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, 30190-002 Belo Horizonte, MG, Brazil. Electronic address: oamfilho@cpqrr.fiocruz.br.
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
mesh_terms: D015415:Biomarkers / Q000097:blood; D018925:Chemokines / Q000097:blood*; D002829:Choroid / Q000473:pathology; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000097:blood*; D006801:Humans; D007223:Infant; D012160:Retina / Q000473:pathology; D014125:Toxoplasmosis, Congenital / Q000276:immunology* / Q000473:pathology; D014126:Toxoplasmosis, Ocular / Q000276:immunology* / Q000473:pathology
chemical_list: D015415:Biomarkers; D018925:Chemokines; D016207:Cytokines
keywords: Biomarkers; Congenital toxoplasmosis; Infants; Retinochoroiditis