title: [X-ray irradiation induces apoptosis of mouse GC1 sperm cells via nuclear translocation of apoptosis-inducing factor].
abstract: Objective To study the effect of X-ray irradiation on the localization of apoptosis inducing factor (AIF) in mouse GC1 sperm cells. Methods After GC1 cells were treated with 0, 3, 6 and 9 Gy X irradiation, BrdU incorporation assay was performed to detect the proliferation of GC1 cells. Forty-eight hours after irradiation, the nuclear condensation was observed by DAPI staining. The subcellular localization of AIF was showed using the immunofluorescence staining, both in the whole cell extracts and in nuclear extracts, and the expression levels of AIF were detected using Western blot analysis. Results With the increase of X-ray irradiation dose, the proliferation of GC1 cells significantly decreased, and the activity of cells was weakened. After 6 Gy irradiation, in nuclear extracts, but not in the whole cell extracts, the protein AIF was upregulated significantly. It meant the nuclear translocation of protein AIF. Conclusion X-ray irradiation induces the apoptosis of mouse GC1 sperm cells, meanwhile, the nuclear translocation of AIF occurs.
authors.affiliation: Ministry-of-Education Key Laboratory of Fertility Preservation and Maintenance, Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.affiliation: Ministry-of-Education Key Laboratory of Fertility Preservation and Maintenance, Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Jingbin
authors.initials: J
authors.lastname: Ding
authors.affiliation: Cancer Hospital, General Hospital, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Physiology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ding
authors.affiliation: Cancer Hospital, General Hospital, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Xinshe
authors.initials: X
authors.lastname: Xia
authors.affiliation: Ministry-of-Education Key Laboratory of Fertility Preservation and Maintenance, Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004, China. *Corresponding author, E-mail: zhaowei@nxmu.edu.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D051033:Apoptosis Inducing Factor / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D021381:Protein Transport / Q000528:radiation effects; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D014965:X-Rays
chemical_list: D051033:Apoptosis Inducing Factor; C494830:AIFM1 protein, mouse