title: High Prevalence and Predominance of theaph(2″)-IfGene Conferring Aminoglycoside Resistance in Campylobacter.
abstract: Campylobacteris a major foodborne pathogen, and previous studies revealed thatCampylobacterisolates from food-producing animals are increasingly resistant to gentamicin in China. The molecular epidemiology and genetic mechanisms responsible for gentamicin resistance in China have not been well understood. In this study, 607Campylobacterisolates of chicken and swine origins collected in 2014 were analyzed, revealing that 15.6% (25/160) of theCampylobacter jejuniisolates and 79.9% (357/447) of theCampylobacter coliisolates were resistant to gentamicin. PCR detection of the gentamicin resistance genes indicated thataph(2″)-Ifwas more prevalent than the previously identifiedaacA/aphDgene and has become the dominant gentamicin resistance determinant inCampylobacterTransformation and whole-genome sequencing as well as long-range PCR discovered thataph(2″)-Ifwas located on a chromosomal segment inserted between two conserved genes, Cj0299 andpanBCloning ofaph(2″)-Ifinto gentamicin-susceptibleC. jejuniNCTC 11168 confirmed its function in conferring high-level resistance to gentamicin and kanamycin. Molecular typing by pulsed-field gel electrophoresis suggested that both regional expansion of a particular clone and horizontal transmission were involved in the dissemination of theaph(2″)-Ifgene inCampylobacterTo our knowledge, this is the first report describing the high prevalence of a chromosomally encodedaph(2″)-Ifgene inCampylobacterThe high prevalence and predominance of this gene might be driven by the use of aminoglycoside antibiotics in food animal production in China and potentially compromise the usefulness of gentamicin as a therapeutic agent forCampylobacter-associated systemic infection.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yao
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Liu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Qijing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China szq@cau.edu.cn.|College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Zhangqi
authors.initials: Z
authors.lastname: Shen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002169:Campylobacter Infections / Q000188:drug therapy / Q000382:microbiology; D017000:Campylobacter coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D059022:Food Safety; D005517:Foodborne Diseases / Q000382:microbiology; D005839:Gentamicins / Q000494:pharmacology*; D007612:Kanamycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D013552:Swine / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D007612:Kanamycin; D017853:Phosphotransferases (Alcohol Group Acceptor); C048564:aminoglycoside 2''-phosphotransferase
keywords: Campylobacter; aph(2″)-If; food safety; gentamicin resistance