title: Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology.
abstract: This study was aimed at investigating the immunomodulating activity of Hericium erinaceus polysaccharide (HEP) in mice, by assessing splenic lymphocyte proliferation (cell-mediated immunity), serum hemolysin levels (humoral immunity), phagocytic capacity of peritoneal cavity phagocytes (macrophage phagocytosis), and NK cell activity. ELISA of immunoglobulin A (SIgA) in the lamina propria, and western blotting of small intestinal proteins were also performed to gain insight into the mechanism by which HEP affects the intestinal immune system. Here, we report that HEP improves immune function by functionally enhancing cell-mediated and humoral immunity, macrophage phagocytosis, and NK cell activity. In addition, HEP was found to upregulate the secretion of SIgA and activate the MAPK and AKT cellular signaling pathways in the intestine. In conclusion, all these results allow us to postulate that the immunomodulatory effects of HEP are most likely attributed to the effective regulation of intestinal mucosal immune activity.
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Yan
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Han
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Guihua
authors.initials: G
authors.lastname: Tai
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Yifa
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. chenghr893@nenu.edu.cn.
authors.forename: Hairong
authors.identifier: http://orcid.org/0000-0001-9194-6026
authors.initials: H
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D005260:Female; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D007070:Immunoglobulin A / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007155:Immunologic Factors; D011134:Polysaccharides