title: Exploratory Studies on the in Vitro Anti-inflammatory Potential of Two Herbal Teas (Annona muricata L. and Jasminum grandiflorum L.), and Relation with Their Phenolic Composition.
abstract: The need of new anti-inflammatory drugs has led to the search for safer and more potent molecules in distinct sources, such as natural products. This work aimed to explore the anti-inflammatory potential of aqueous extracts from two herbal teas (Annona muricata L. and Jasminum grandiflorum L.) in RAW 264.7 macrophages cells and in cell-free assays. Furthermore, the phenolic composition of both extracts and of their hydrolysates was characterized by HPLC-DAD, in order to establish possible relationships with the biological activity. In a general way, A. muricata displayed a stronger capacity to inhibit nitric oxide (NO) production and the activity of phospholipase A2(PLA2), displaying an IC50value of 142 μg/ml against this enzyme. A deeper look at phenolic compounds revealed that aglycones had more capacity to inhibit NO and PLA2than their corresponding glycosides, quercetin being clearly the most potent one (IC50= 7.47 and 1.36 μm, respectively). In addition, 5-O-caffeoylquinic acid, at 1.56 μm, could also inhibit PLA2(ca. 35%). Our findings suggest that the consumption of both herbal teas may be a preventive approach to inflammatory disorders.
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, no. 228, PT-4050-313, Porto.
authors.forename: Andreia P
authors.initials: AP
authors.lastname: Oliveira
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, no. 228, PT-4050-313, Porto.
authors.forename: Ivone
authors.initials: I
authors.lastname: Sá
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, no. 228, PT-4050-313, Porto.
authors.forename: David M
authors.initials: DM
authors.lastname: Pereira
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, no. 228, PT-4050-313, Porto.
authors.forename: Rui F
authors.initials: RF
authors.lastname: Gonçalves
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, no. 228, PT-4050-313, Porto.
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, no. 228, PT-4050-313, Porto.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
mesh_terms: D000818:Animals; D031041:Annona / Q000737:chemistry; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002474:Cell-Free System / Q000187:drug effects; D031660:Jasminum / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D064801:Phospholipase A2 Inhibitors; D010936:Plant Extracts; D000067996:RAW 264.7 Cells; D000069459:Teas, Herbal
chemical_list: D000893:Anti-Inflammatory Agents; D010636:Phenols; D064801:Phospholipase A2 Inhibitors; D010936:Plant Extracts; D000069459:Teas, Herbal; D009569:Nitric Oxide
keywords: 
  Annona muricata
  ; 
  Jasminum grandiflorum
  ; Anti-inflammatory activity; Phospholipase A2; RAW 264.7