title: Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains.
abstract: Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Bücherl
authors.affiliation: Ludwig-Maximilians-Universität München, Institute of Genetics, Martinsried, Germany.
authors.forename: Iris K
authors.initials: IK
authors.lastname: Jarsch
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Christian
authors.identifier: 0000-0003-1157-1354
authors.initials: C
authors.lastname: Schudoma
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Cécile
authors.identifier: 0000-0002-5537-7556
authors.initials: C
authors.lastname: Segonzac
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Malick
authors.initials: M
authors.lastname: Mbengue
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Silke
authors.initials: S
authors.lastname: Robatzek
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Daniel
authors.identifier: 0000-0003-1032-0887
authors.initials: D
authors.lastname: MacLean
authors.affiliation: Ludwig-Maximilians-Universität München, Institute of Genetics, Martinsried, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ott
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Cyril
authors.identifier: 0000-0003-4935-8583
authors.initials: C
authors.lastname: Zipfel
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry*; D000069416:Intravital Microscopy; D011494:Protein Kinases / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis*; D062211:Spatio-Temporal Analysis
chemical_list: D029681:Arabidopsis Proteins; D011956:Receptors, Cell Surface; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis; C108270:BRI1 protein, Arabidopsis
keywords: A. thaliana; cell biology; growth; immunity; microdomain; plant biology; plasma membrane; receptor kinase; signaling