title: Hepatotoxicity of benzotriazole and its effect on the cadmium induced toxicity in zebrafish Danio rerio.
abstract: As an emerging contaminant, 1-H-benzotriazole (1H-BTR) has been detected in the engineered and natural aquatic environments, which usually coexists with heavy metals and causes combined pollution. In the present study, wild-type and transgenic zebrafish Danio rerio were used to explore the acute toxicity as well as the single and joint hepatotoxicity of cadmium (Cd) and 1H-BTR. Although the acute toxicity of 1H-BTR to zebrafish was low, increased expression of liver-specific fatty acid binding protein was observed in transgenic zebrafish when the embryos were exposed to 5.0 μM of 1H-BTR for 30 days. Besides, co-exposure to 1H-BTR not only reduced the acute toxic effects induced by Cd, but also alleviated the Cd-induced liver atrophy in transgenic fish. Correspondingly, effects of combined exposure to 1H-BTR on the Cd-induced expressions of several signal pathway-related genes and superoxide dismutase and glutathione-s-transferase proteins were studied. Based on the determination of Cd bioaccumulation in fish and the complexing stability constant (β) of Cd-BTR complex in solution, the detoxification mechanism of co-existing 1H-BTR on Cd to the zebrafish was discussed.
authors.affiliation: School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Duan
authors.affiliation: Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Nankai University, Tianjin 300071, China.
authors.forename: Yanshuai
authors.initials: Y
authors.lastname: Xing
authors.affiliation: School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Zhitong
authors.initials: Z
authors.lastname: Feng
authors.affiliation: School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Nankai University, Tianjin 300071, China. Electronic address: wang2007@nankai.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Nankai University, Tianjin 300071, China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D008658:Inactivation, Metabolic; D008099:Liver / Q000187:drug effects*; D019216:Metals, Heavy / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014230:Triazoles / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism
chemical_list: D019216:Metals, Heavy; D014230:Triazoles; D002104:Cadmium; C012771:benzotriazole; D013482:Superoxide Dismutase
keywords: Benzotriazole; Cadmium; Complexation; Hepatotoxicity; Zebrafish