title: Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins.
abstract: Localization of mRNA is important in a number of cellular processes such as embryogenesis, cellular motility, polarity, and a variety of neurological processes. A synthetic device that controls cellular mRNA localization would facilitate investigations on the significance of mRNA localization in cellular function and allow an additional level of controlling gene expression. In this work, we developed the PUF (Pumilio and FBF homology domain)-assisted localization of RNA (PULR) system, which utilizes a eukaryotic cell's cytoskeletal transport machinery to reposition mRNA within a cell. Depending on the cellular motor used, we show ligand-dependent transport of mRNA toward either pole of the microtubular network of cultured cells. In addition, implementation of the reprogrammable PUF domain allowed the transport of untagged endogenous mRNA in primary neurons.
authors.affiliation: Department of Biochemistry, University of Illinois at Urbana-Champaign , 600 South Mathews Avenue, Urbana, Illinois 61801, United States.
authors.forename: Zhanar
authors.identifier: 0000-0001-9550-2633
authors.initials: Z
authors.lastname: Abil
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University , Padualaan 8, 3584CH Utrecht, The Netherlands.
authors.forename: Laura F
authors.initials: LF
authors.lastname: Gumy
authors.affiliation: Department of Biochemistry, University of Illinois at Urbana-Champaign , 600 South Mathews Avenue, Urbana, Illinois 61801, United States.|Department of Chemical and Biomolecular Engineering, Department of Bioengineering, Department of Chemistry, and Institute for Genomic Biology, University of Illinois at Urbana-Champaign , 600 South Mathews Avenue, Urbana, Illinois 61801, United States.
authors.forename: Huimin
authors.identifier: 0000-0002-9069-6739
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University , Padualaan 8, 3584CH Utrecht, The Netherlands.
authors.forename: Casper C
authors.initials: CC
authors.lastname: Hoogenraad
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002478:Cells, Cultured; D004398:Dyneins / Q000737:chemistry; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000378:metabolism; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D000199:Actins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D004398:Dyneins; D016547:Kinesins
keywords: Pumilio and fem3 mRNA-binding factor (PUF); RNA-binding proteins (RBP); dynein; kinesin; mRNA transport