title: Changes in the microbial community of an anammox consortium during adaptation to marine conditions revealed by 454 pyrosequencing.
abstract: The anammox activity of a freshwater anammox consortium was strongly inhibited at low-salinity level. Stepwise adaptation from 0 to 3 g NaCl L-1took 153 days. Further adaptation to high-salinity concentration (from 3 to 30 g L-1) took only 40 days, and no inhibition was observed. A comprehensive insight into the salinity-induced successions of the total and the anammox communities was obtained by 454 pyrosequencing of 16S rRNA gene amplicons and statistical analysis. A major succession in the anammox community was observed at 3 g L-1where the dominating population shifted from Candidatus Brocadia fulgida to Ca. Kuenenia stuttgartiensis. The latter dominated at high salinity and seemed to be essential for the high (˃96%) ammonium and nitrite removal efficiencies achieved. SIMPER analysis indicated that these two dominating anammox species explained most to the differences in community structure among samples and helped in identifying other important members at different salinities.
authors.affiliation: Department of Biotechnology, Faculty of Natural Sciences and Technology, NTNU Norwegian University of Science and Technology, Sem Saelands v. 6/8, 7491, Trondheim, Norway. blanca.g.silva@ntnu.no.
authors.forename: Blanca M
authors.initials: BM
authors.lastname: Gonzalez-Silva
authors.affiliation: Department of Biotechnology, Faculty of Natural Sciences and Technology, NTNU Norwegian University of Science and Technology, Sem Saelands v. 6/8, 7491, Trondheim, Norway.
authors.forename: Are J
authors.initials: AJ
authors.lastname: Rønning
authors.affiliation: Department of Biotechnology, Faculty of Natural Sciences and Technology, NTNU Norwegian University of Science and Technology, Sem Saelands v. 6/8, 7491, Trondheim, Norway.
authors.forename: Ingrid K
authors.initials: IK
authors.lastname: Andreassen
authors.affiliation: Department of Biotechnology, Faculty of Natural Sciences and Technology, NTNU Norwegian University of Science and Technology, Sem Saelands v. 6/8, 7491, Trondheim, Norway.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Bakke
authors.affiliation: División de Ciencias Ambientales, Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa San José 2055, Col. Lomas 4a Seccion, 78216, San Luis Potosi, SLP, Mexico.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Cervantes
authors.affiliation: Department of Biotechnology, Faculty of Natural Sciences and Technology, NTNU Norwegian University of Science and Technology, Sem Saelands v. 6/8, 7491, Trondheim, Norway.
authors.forename: Kjetill
authors.initials: K
authors.lastname: Østgaard
authors.affiliation: Department of Biotechnology, Faculty of Natural Sciences and Technology, NTNU Norwegian University of Science and Technology, Sem Saelands v. 6/8, 7491, Trondheim, Norway.
authors.forename: Olav
authors.initials: O
authors.lastname: Vadstein
mesh_terms: D000064:Acclimatization; D064751:Ammonium Compounds / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D005618:Fresh Water / Q000382:microbiology; D059014:High-Throughput Nucleotide Sequencing; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D064751:Ammonium Compounds; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: Amplicon pyrosequencing; Anammox; Bacterial community; Salinity