title: Determining the optimal protocol for preparing an acellular scaffold of tissue engineered small-diameter blood vessels.
abstract: Although detergent-based decellularization protocols have been widely used to obtain a natural extracellular matrix (ECM) scaffold in tissue engineering, some key challenges still exist. To achieve an optimum natural decellularized scaffold for the construction of tissue-engineered small-diameter blood vessels (TEBV), porcine carotid arteries (PCAs) were decellularized by combining sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC) and Triton X-100 (Triton) in different concentrations. Tissue samples were processed and their histological, biochemical and biomechanical characteristics were investigated. Results showed that only two methods 0.5% (SDS + SDC) and 1% (SDS + SDC) could completely remove of the cellular contents and preserve the native ECM architecture. Furthermore, 1% (SDS + SDC) based methods acquire preferable porosity and suitable mechanical strength. Residual Triton in the ECM scaffold holds intensive cytotoxity. In conclusion, 1%(SDS + SDC) based method can obtain a superior PCAs scaffold for the construction of TEBV. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 619-631, 2018.
authors.affiliation: Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Pu
authors.affiliation: Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.|Cardiovascular Surgery, Institution of Yunnan, Kunming, Yunnan, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Xingna
authors.initials: X
authors.lastname: Pan
authors.affiliation: Central Laboratory, Yan'an Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Zongliu
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.|Cardiovascular Surgery, Institution of Yunnan, Kunming, Yunnan, People's Republic of China.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Chen
authors.affiliation: Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.|Cardiovascular Surgery, Institution of Yunnan, Kunming, Yunnan, People's Republic of China.
authors.forename: Zhuhui
authors.initials: Z
authors.lastname: Na
authors.affiliation: Central Laboratory, Yan'an Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Mingyao
authors.initials: M
authors.lastname: Meng
authors.affiliation: Department of Pathology, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Pathology, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Liqiong
authors.initials: L
authors.lastname: Wang
authors.affiliation: Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.|Cardiovascular Surgery, Institution of Yunnan, Kunming, Yunnan, People's Republic of China.
authors.forename: Yaxiong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Cardiovascular Surgery, Yan'an Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.|Cardiovascular Surgery, Institution of Yunnan, Kunming, Yunnan, People's Republic of China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002339:Carotid Arteries / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D003840:Deoxycholic Acid / Q000737:chemistry / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D017830:Octoxynol / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry / Q000494:pharmacology; D013552:Swine; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol
keywords: decellularization; extracellular matrix; small-diameter blood vessels; tissue engineering