title: Portable Infrared Laser Spectroscopy for On-site Mycotoxin Analysis.
abstract: Mycotoxins are toxic secondary metabolites of fungi that spoil food, and severely impact human health (e.g., causing cancer). Therefore, the rapid determination of mycotoxin contamination including deoxynivalenol and aflatoxin B1in food and feed samples is of prime interest for commodity importers and processors. While chromatography-based techniques are well established in laboratory environments, only very few (i.e., mostly immunochemical) techniques exist enabling direct on-site analysis for traders and manufacturers. In this study, we present MYCOSPEC - an innovative approach for spectroscopic mycotoxin contamination analysis at EU regulatory limits for the first time utilizing mid-infrared tunable quantum cascade laser (QCL) spectroscopy. This analysis technique facilitates on-site mycotoxin analysis by combining QCL technology with GaAs/AlGaAs thin-film waveguides. Multivariate data mining strategies (i.e., principal component analysis) enabled the classification of deoxynivalenol-contaminated maize and wheat samples, and of aflatoxin B1affected peanuts at EU regulatory limits of 1250 μg kg-1and 8 μg kg-1, respectively.
authors.affiliation: Ulm University, Institute of Analytical and Bioanalytical Chemistry, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Sieger
authors.affiliation: McGill University, Atmospheric and Oceanic Sciences, 805 Sherbrooke Street West, Montreal, QC, H3A 0B9, Montreal, Canada.
authors.forename: Gregor
authors.initials: G
authors.lastname: Kos
authors.affiliation: University of Natural Resources and Applied Life Sciences, IFA-Tulln, Konrad Lorenz Straße 20, A-3430 Tulln, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Sulyok
authors.affiliation: MG Optical Solutions GmbH, Industriestr. 23, 86919 Utting/Ammersee, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Godejohann
authors.affiliation: University of Natural Resources and Applied Life Sciences, IFA-Tulln, Konrad Lorenz Straße 20, A-3430 Tulln, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Krska
authors.affiliation: Ulm University, Institute of Analytical and Bioanalytical Chemistry, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D010367:Arachis / Q000737:chemistry*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods; D007834:Lasers; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods
chemical_list: D016604:Aflatoxin B1