title: Development of a paper-based microfluidic analytical device by a more facile hydrophobic substrate generation strategy.
abstract: Microfluidic paper-based analytical devices (μPADs) have a significant potential in developing portable and disposable point-of-care testing (POCT). Herein, a facile, rapid, cost-effective and environment friendly strategy for μPADs fabrication is proposed. Specifically, the substrate paper was hydrophobized by coating with trimethoxysilane (TOS), and then the selected area was hydrophilized by treating with surfactant. The whole fabrication process was implemented within 7 min, with no need for complex pre-treatment, high-temperature and special equipment. As a proof-of-concept application, the as-prepared μPAD was applied to determination of the glucose content in human serum samples. The results agreed well with those obtained by a glucometer. We believe that the μPADs fabrication method proposed here could provide a facile, rapid and low-cost reference for other related studies.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Xue
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Meng-Yue
authors.initials: MY
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Zhu
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Yan
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: wanglong79@nwsuaf.edu.cn.
authors.forename: Jian-Long
authors.initials: JL
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: zhangdh@nwsuaf.edu.cn.
authors.forename: Dao-Hong
authors.initials: DH
authors.lastname: Zhang
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D004867:Equipment Design; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010209:Paper; D019095:Point-of-Care Systems
chemical_list: D001786:Blood Glucose
keywords: Colorimetric assay; Glucose; POCT; TOS; μPAD