title: Autocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin.
abstract: Unusual intramolecular cross-links present in adhesins from Gram-positive bacteria have been used to develop a generic process amenable to biotechnology applications. Based on the crystal structure of RrgA, the Streptococcus pneumoniae pilus adhesin, we provide evidence that two engineered protein fragments retain their ability to associate covalently with high specificity, in vivo and in vitro, once isolated from the parent protein. We determined the optimal conditions for the assembly of the complex and we solved its crystal structure at 2 Å. Furthermore, we demonstrate biotechnological applications related to antibody production, nanoassembly and cell-surface labeling based on this process we named Bio Molecular Welding.
authors.affiliation: Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: J
authors.initials: J
authors.lastname: Bonnet
authors.affiliation: Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: J
authors.initials: J
authors.lastname: Cartannaz
authors.affiliation: Institut de Biosciences et Biotechnologies de Grenoble (BIG), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: G
authors.initials: G
authors.lastname: Tourcier
authors.affiliation: Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: C
authors.initials: C
authors.lastname: Contreras-Martel
authors.affiliation: Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Kleman
authors.affiliation: Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: C
authors.initials: C
authors.lastname: Morlot
authors.affiliation: Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: T
authors.initials: T
authors.lastname: Vernet
authors.affiliation: Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Di Guilmi
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D036781:Fimbriae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008970:Molecular Weight; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins; D021241:Spectrometry, Mass, Electrospray Ionization; D037521:Virulence Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D046912:Multiprotein Complexes; D011993:Recombinant Fusion Proteins; C579340:RrgA protein, Streptococcus pneumoniae; D037521:Virulence Factors; D036781:Fimbriae Proteins