title: Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans.
abstract: In Caenorhabditis elegans, the AWC neurons are thought to deploy a cGMP signaling cascade in the detection of and response to AWC sensed odors. Prolonged exposure to an AWC sensed odor in the absence of food leads to reversible decreases in the animal's attraction to that odor. This adaptation exhibits two stages referred to as short-term and long-term adaptation. Previously, the protein kinase G (PKG), EGL-4/PKG-1, was shown necessary for both stages of adaptation and phosphorylation of its target, the beta-type cyclic nucleotide gated (CNG) channel subunit, TAX-2, was implicated in the short term stage. Here we uncover a novel role for the CNG channel subunit, CNG-3, in short term adaptation. We demonstrate that CNG-3 is required in the AWC for adaptation to short (thirty minute) exposures of odor, and contains a candidate PKG phosphorylation site required to tune odor sensitivity. We also provide in vivo data suggesting that CNG-3 forms a complex with both TAX-2 and TAX-4 CNG channel subunits in AWC. Finally, we examine the physiology of different CNG channel subunit combinations.
authors.affiliation: Department of Biological Sciences, The George Washington University, Science and Engineering Hall 6000, 800 22nd St N.W., 20052, Washington DC, USA. damienoh@gwu.edu.|Institute for Neuroscience, The George Washington University, 636 Ross Hall, 2300 I St. N.W., 20052, Washington DC, USA. damienoh@gwu.edu.
authors.forename: Damien M
authors.identifier: 0000-0001-5752-2131
authors.initials: DM
authors.lastname: O'Halloran
authors.affiliation: UCSF Diabetes Center, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Cell and Tissue Biology, University of California, 35 Medical Center Way, San Francisco, CA, 94143, USA.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Altshuler-Keylin
authors.affiliation: Department of Internal Medicine, Davis, 451 E Health Science Drive, Genome & Biomedical Sciences Facility, University of California, Davis, CA, 95616, USA.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Zhang
authors.affiliation: Department of Biological Sciences, The George Washington University, Science and Engineering Hall 6000, 800 22nd St N.W., 20052, Washington DC, USA.|Institute for Neuroscience, The George Washington University, 636 Ross Hall, 2300 I St. N.W., 20052, Washington DC, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.affiliation: Center for Neuroscience, University of California, Davis, 1544 Newton Ct., Davis, CA, 95616, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Morales-Phan
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Gladstone Institute of Neurological Disease, University of California, San Francisco, CA, 94158, USA.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Kaye
authors.affiliation: Kavli Institute for Fundamental Neuroscience and Department of Cell and Tissue Biology, University of California, 35 Medical Center Way, San Francisco, CA, 94143, USA.
authors.forename: Chantal
authors.initials: C
authors.lastname: Brueggemann
authors.affiliation: Center for Neuroscience, University of California, Davis, 1544 Newton Ct., Davis, CA, 95616, USA.|Department of Neurology, University of California, Davis, 4860 Y Street, Sacramento, CA, 95817, USA.
authors.forename: Tsung-Yu
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Kavli Institute for Fundamental Neuroscience and Department of Cell and Tissue Biology, University of California, 35 Medical Center Way, San Francisco, CA, 94143, USA. noelle.letoile@ucsf.edu.
authors.forename: Noelle D
authors.initials: ND
authors.lastname: L'Etoile
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D009473:Neuronal Plasticity; D009832:Olfactory Nerve / Q000166:cytology* / Q000378:metabolism; D010766:Phosphorylation; D012903:Smell
chemical_list: C000628134:CNG-3 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D054815:Cyclic Nucleotide-Gated Cation Channels; D007473:Ion Channels; C102183:tax-2 protein, C elegans; C102184:tax-4 protein, C elegans; D017869:Cyclic GMP-Dependent Protein Kinases