title: FlexPlex27-highly multiplexed rapid DNA identification for law enforcement, kinship, and military applications.
abstract: Rapid DNA identification is the use of a rugged, field-deployable system to generate short tandem repeat (STR) profiles in law enforcement, military, immigration, and homeland security applications. A performance verification study was conducted on the ANDE Rapid DNA identification system using FlexPlex27, a highly multiplexed, 27 locus assay that generates data for the expanded CODIS core loci and all additional STR loci required for international databasing. The assay contains 23 autosomal loci (D1S1656, D2S1338, D2S441, D3S1358, D5S81, D6S1043, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, CSF1PO, Penta E, TH01, vWA, TPOX, and SE33), three Y-chromosomal loci (DYS391, DYS576, and DYS570), and Amelogenin. Study results demonstrate that the instrument is reliable, reproducible, accurate, robust, and ready for a large scale, comprehensive developmental validation by NDIS-participating laboratories. The additional loci in the FlexPlex assay allow for improved STR profile sharing globally, increase the power of discrimination for identification matches, and improve the effectiveness of kinship analyses.
authors.affiliation: ANDE, 266 Second Avenue, Waltham, MA, 02451, USA.
authors.forename: Ranjana
authors.initials: R
authors.lastname: Grover
authors.affiliation: ANDE, 266 Second Avenue, Waltham, MA, 02451, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: ANDE, 266 Second Avenue, Waltham, MA, 02451, USA.
authors.forename: Rosemary S
authors.initials: RS
authors.lastname: Turingan
authors.affiliation: ANDE, 266 Second Avenue, Waltham, MA, 02451, USA.
authors.forename: Julie L
authors.initials: JL
authors.lastname: French
authors.affiliation: ANDE, 266 Second Avenue, Waltham, MA, 02451, USA.
authors.forename: Eugene
authors.initials: E
authors.lastname: Tan
authors.affiliation: ANDE, 266 Second Avenue, Waltham, MA, 02451, USA. rfs4n6@ANDE.com.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Selden
mesh_terms: D053523:Amelogenin / Q000235:genetics; D000818:Animals; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000295:instrumentation*; D030561:Databases, Nucleic Acid; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci; D015894:Genome, Human; D006077:Government Agencies; D006801:Humans; D038622:Internationality; D008297:Male; D018895:Microsatellite Repeats; D009061:Mouth Mucosa / Q000166:cytology; D010375:Pedigree; D012984:Software; D013045:Species Specificity; D013048:Specimen Handling; D013997:Time Factors
chemical_list: D053523:Amelogenin
keywords: CODIS 20; Kinship; Rapid DNA identification; Short tandem repeat