title: Cellular factories for coenzyme Q10production.
abstract: Coenzyme Q10(CoQ10), a benzoquinone present in most organisms, plays an important role in the electron-transport chain, and its deficiency is associated with various neuropathies and muscular disorders. CoQ10is the only lipid-soluble antioxidant found in humans, and for this, it is gaining popularity in the cosmetic and healthcare industries. To meet the growing demand for CoQ10, there has been considerable interest in ways to enhance its production, the most effective of which remains microbial fermentation. Previous attempts to increase CoQ10production to an industrial scale have thus far conformed to the strategies used in typical metabolic engineering endeavors. However, the emergence of new tools in the expanding field of synthetic biology has provided a suite of possibilities that extend beyond the traditional modes of metabolic engineering. In this review, we cover the various strategies currently undertaken to upscale CoQ10production, and discuss some of the potential novel areas for future research.
authors.affiliation: Department of Biochemistry, National University of Singapore, Singapore, Singapore.
authors.forename: Sean Qiu En
authors.initials: SQ
authors.lastname: Lee
authors.affiliation: School of Chemical & Life Sciences, Nanyang Polytechnic, Singapore, Singapore.
authors.forename: Tsu Soo
authors.initials: TS
authors.lastname: Tan
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
authors.affiliation: Department of Biochemistry, National University of Singapore, Singapore, Singapore. bchces@nus.edu.sg.|National University Health System (NUHS), Singapore, Singapore. bchces@nus.edu.sg.|NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, Singapore. bchces@nus.edu.sg.|NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore. bchces@nus.edu.sg.
authors.forename: Ee Sin
authors.initials: ES
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D060847:Metabolic Engineering / Q000379:methods; D015202:Protein Engineering / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D014815:Vitamins / Q000096:biosynthesis*
chemical_list: D000975:Antioxidants; D014815:Vitamins; D014451:Ubiquinone; C024989:coenzyme Q10
keywords: Antioxidant; Coenzyme Q10; Industrial biosynthesis; Isoprenoid; Protein engineering; Synthetic biology