title: Assessment of in vivo experiments: The newly synthesized porphyrin with proper light source enhanced effectiveness of PDT comparing to 5-ALA-mediated PDT.
abstract: BACKGROUND
  The search for new photosensitizers for application in photodynamic therapy has quite a long history. In the past, a large number of potent photosensitizers were used in both basic and clinical studies; however, only a few turned out to be effective and safe.
  
  
  METHODS
  In the present study, two compounds were used: 5-aminolevulinic acid in two formulations (free and liposomal), and the newly synthesized porphyrin, 5,10,15,20-tetra-p-tolyl-22,24-dithiadibenzocarbaporphyrin, termed DTDB. Two different light sources, a halogen lamp (wavelength 450+/-20nm) and a diode laser (wavelength 450nm), were used to sensitize the compounds. The entire experiment was performed on mice bearing mouse mammary carcinoma, 4T1.
  
  
  RESULTS
  The results showed that the DTDB-PDT applied by means of a laser proved to be most effective and caused the 83.3% necrosis of treated tumors. The overall effect of laser PDT was more potent than that of the halogen lamp-mediated PDT.
  
  
  CONCLUSIONS
  In the present study, we would like to show that modifications of porphyrins lead to an increase in the effectiveness of PDT and that this effect could also be potentiated by using a proper light source.
authors.affiliation: Department of Oral Surgery, Wrocław Medical University, 26 Krakowska, 50-425 Wrocław, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Jurczyszyn
authors.affiliation: Department of Pathology, Wrocław Medical University, 1 Marcinkowskiego, 50-367, Wrocław, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Woźniak
authors.affiliation: Department of Pathology, Wrocław Medical University, 1 Marcinkowskiego, 50-367, Wrocław, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Symonowicz
authors.affiliation: Department of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383, Wrocław, Poland.
authors.forename: Natasza
authors.initials: N
authors.lastname: Sprutta
authors.affiliation: Department of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383, Wrocław, Poland.
authors.forename: Lechosław
authors.initials: L
authors.lastname: Latos-Grażyński
authors.affiliation: Department of Pathology, Wrocław Medical University, 1 Marcinkowskiego, 50-367, Wrocław, Poland. Electronic address: ziolkows@interia.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ziółkowski
authors.affiliation: Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska, 01-142 Warsaw, Poland.
authors.forename: Yurij
authors.initials: Y
authors.lastname: Ivonyak
authors.affiliation: Accuro Ltd., Kolejowa 15/17, 01-217 Warszawa, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Tuchowski
authors.affiliation: Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska, 01-142 Warsaw, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Trzeciakowski
mesh_terms: D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D008027:Light; D008029:Lighting / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000473:pathology* / Q000532:radiotherapy*; D010778:Photochemotherapy / Q000295:instrumentation* / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011166:Porphyrins / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; D000622:Aminolevulinic Acid
keywords: 5,10,15,20-Tetra-p-tolyl-22,24-dithiadibenzocarbaporphyrin; 5-Aminolevulinic acid; Histopathology; Laser; Liposomal-5-aminolevulinic acid