title: Profiling Proteins in the Hypothalamus and Hippocampus of a Rat Model of Premenstrual Syndrome Irritability.
abstract: Premenstrual syndrome (PMS) refers to several physical and mental symptoms (such as irritability) commonly encountered in clinical gynaecology. The incidence of PMS has been increasing, attracting greater attention from medical fields. However, PMS pathogenesis remains unclear. This study employed two-dimensional gel electrophoresis (2DE) for proteomic map analysis of the hypothalamus and hippocampus of rat models of premenstrual syndrome (PMS) irritability. Matrix-assisted laser desorption/ionisation time of flight mass spectroscopy (MALDI-TOF-MS) was used to identify proteins possibly related with PMS irritability. Baixiangdan, a traditional Chinese medicine effective against PMS irritability, was used in the rat model to study putative target proteins of this medicine. The hypothalamus and hippocampus of each group modelling PMS displayed the following features: decreased expression of Ulip2, tubulin beta chain 15,αactin, and interleukin 1 receptor accessory protein; increased expression of kappa-B motif-binding phosphoprotein; decreased expression of hydrolase at the end of ubiquitin carboxy, albumin, and aldolase protein; and increased expression of M2 pyruvate kinase, panthenol-cytochrome C reductase core protein I, and calcium-binding protein. Contrasting with previous studies, the current study identified new proteins related to PMS irritability. Our findings contribute to understanding the pathogenesis of PMS irritability and could provide a reference point for further studies.
authors.affiliation: Key Laboratory of Traditional Chinese Medicine for Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Mingqi
authors.identifier: 0000-0001-7310-1206
authors.initials: M
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Traditional Chinese Medicine for Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Peng
authors.identifier: 0000-0003-4117-4165
authors.initials: P
authors.lastname: Sun
authors.affiliation: Laboratory of Ethnopharmacology, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yang
authors.identifier: 0000-0002-1225-6017
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Traditional Chinese Medicine for Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wei
authors.affiliation: Technical Office of Pharmacology, Shandong Institute for Food and Drug Control, Jinan 250351, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wei
authors.affiliation: Key Laboratory of Traditional Chinese Medicine for Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Chunhong
authors.identifier: 0000-0001-6976-6213
authors.initials: C
authors.lastname: Song
authors.affiliation: College of Psychology, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Fushun
authors.identifier: 0000-0002-2010-6254
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Traditional Chinese Medicine for Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Jibiao
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D011293:Premenstrual Syndrome / Q000188:drug therapy / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal