title: Cis-bifenthrin induces immunotoxicity in adolescent male C57BL/6 mice.
abstract: Bifenthrin (BF) is an important synthetic pyrethroid. Previous studies have demonstrated that cis-BF exhibits toxic effects on development, the neurological, reproductive and endocrine system. In this study, we evaluated the immunotoxicity caused by cis-BF in adolescent male C57BL/6 mice. Mice were exposed orally to 0, 5, 10, and 20 mg/kg/d for 3 weeks. The results showed that body weight, spleen weight, and splenic cellularity decreased in mice exposed to 20 mg/kg/d cis-BF. Additionally, we found that the mRNA levels of the pro-inflammatory factors IL-1β, IL-6, CXCL-1, and TNF-α, in peritoneal macrophages, the spleen, and the thymus were inhibited in the cis-BF-treated groups. Moreover, MTT assays demonstrated that cis-BF inhibited splenocyte proliferation stimulated by LPS or Con A, as well as the secretion of IFN-γ on Con A stimulation. Collectively, the results of this study suggest that exposure to cis-BF has the potential to induce immunotoxicity in adolescent male C57BL/6 mice.
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Xia
authors.identifier: http://orcid.org/0000-0003-2085-0152
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Bingnan
authors.initials: B
authors.lastname: He
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Lou
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Hu
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Yuanxiang
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D054360:Chemokine CXCL1 / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D054360:Chemokine CXCL1; C475895:Cxcl1 protein, mouse; D053583:Interleukin-1beta; D015850:Interleukin-6; D010575:Pesticides; D011722:Pyrethrins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C099952:bifenthrin
keywords: adolescent male C57BL/6 mice; cis-bifenthrin; immunotoxicity; pro-inflammatory cytokines