title: Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding.
abstract: Biomedical application of selenium nanoparticles (SeNPs) demands the eco-friendly composite for synthesis of SeNPs. The present study reports an aqueous extract of Allium sativum (AqEAS) plug-up the current need. Modern spectroscopic, microscopic and gravimetric techniques were employed to characterize the synthesized nanoparticles. Characterization studies revealed the formation of crystalline spherical shaped SeNPs. FTIR spectrum brings out the presence of different functional groups in AqEAS, which influence the SeNPs formation and stabilization. Furthermore the different aspects of the interaction between SeNPs and CT-DNA were scrutinized by various spectroscopic and cyclic voltametric studies. The results reveals the intercalation and groove binding mode of interaction of SeNPs with stacked base pair of CT-DNA. The Stern-Volmer quenching constant (KSV) were found to be 7.02×106M-1(ethidium bromide), 4.22×106M-1(acridine orange) and 7.6×106M-1(Hoechst) indicating strong binding of SeNPs with CT-DNA. The SeNPs - CT-DNA interactions were directly visualized by atomic force microscopy. The present study unveils the cost effective, innocuous, highly stable SeNPs intricate mechanism of DNA interaction, which will be a milestone in DNA targeted chemotherapy.
authors.affiliation: Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India.
authors.forename: Preedia Babu
authors.initials: PB
authors.lastname: Ezhuthupurakkal
authors.affiliation: Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India.
authors.forename: Lokeswara Rao
authors.initials: LR
authors.lastname: Polaki
authors.affiliation: Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Suyavaran
authors.affiliation: Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India.
authors.forename: Ariraman
authors.initials: A
authors.lastname: Subastri
authors.affiliation: Department of Chemistry, Periyar University, Salem 636011, India.
authors.forename: Venugopal
authors.initials: V
authors.lastname: Sujatha
authors.affiliation: Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India. Electronic address: tchinnasamy@hotmail.com.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Thirunavukkarasu
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005737:Garlic / Q000737:chemistry* / Q000378:metabolism; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009691:Nucleic Acid Denaturation; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D012643:Selenium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D007364:Intercalating Agents; D010936:Plant Extracts; D004247:DNA; C427203:calf thymus DNA; D012643:Selenium
keywords: Atomic force microscopy; DNA interaction; Selenium nanoparticles; Spectroscopy