title: Effects of miRNA-143 and the non-coding RNA MALAT1 on the pathogenesis and metastasis of HeLa cells.
abstract: Cervical cancer is a common female malignancy of global dimensions. MicroRNAs (miRNAs) play crucial roles in the development, differentiation, proliferation, and apoptosis of tumors. The non-coding RNA MALAT1 participates in various physiological processes that are important for proper functioning of the body. Here, we analyzed the expression of miRNA-143 and MALAT1 in HeLa cells to evaluate their roles in the occurrence and metastasis of cervical cancer. HeLa cells were divided into five groups depending on the treatment conditions, namely, transfected with miRNA-143, MALAT1, miRNA-143 inhibitor and the MALAT1 inhibitor, and the untreated control. Reverse transcription-polymerase chain reaction was used to analyze the expression of miRNA-143 and MALAT1, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess proliferation, the trans-well assay to study cell invasion and migration, and western blot to analyze the levels of E-cadherin and vimentin. The proliferation of HeLa cells increased upon treatment with the miRNA-143 inhibitor and decreased when treated with the MALAT1 inhibitor, compared to the proliferation of the groups that were transfected with miRNA-143 and MALAT1, respectively (P < 0.05). Thus, miRNA-143 decreased cell invasion and migration potency, downregulated vimentin and upregulated E-cadherin expression, while MALAT1 had the opposite effects. In conclusion, the low expression of miRNA-143 and high expression of MALAT1 in cervical cancer cells could possibly potentiate cell invasion/migration and alter the levels of vimentin and E-cadherin.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: H E X D
authors.initials: HE
authors.lastname: Niyazi
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: H R
authors.initials: HR
authors.lastname: Zhao
authors.affiliation: Central Laboratory, Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: X P
authors.initials: XP
authors.lastname: Cao
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: M N S
authors.initials: MN
authors.lastname: Abudula
authors.affiliation: Central Laboratory, Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Ye
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: S A
authors.initials: SA
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: R H M
authors.initials: RH
authors.lastname: Yiming
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: W P
authors.initials: WP
authors.lastname: Su
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.affiliation: Central Laboratory, Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: N
authors.initials: N
authors.lastname: Miao
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China baoyongxingsa@163.com.
authors.forename: Y X
authors.initials: YX
authors.lastname: Bao
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D062085:RNA, Long Noncoding / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; C557041:MALAT1 long non-coding RNA, human; C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D014746:Vimentin