title: High quality draft genome sequence of an extremely halophilic archaeonNatrinema altunensestrain AJ2T.
abstract: Natrinema altunensestrain AJ2T, a halophilic archaeal strain, was isolated from a high-altitude (3884 m) salt lake in Xinjiang, China. This strain requires at least 1.7 M NaCl to grow and can grow anaerobically in the presence of nitrate. To understand the genetics underlying its extreme phenotype, wede novoassembled the entire genome sequence of AJ2T(=CGMCC 1.3731T=JCM 12890T). We assembled 3,774,135 bp of a total of 4.4 Mb genome in only 20 contigs and noted its high GC content (64.6%). Subsequently we predicted the gene content and generated genome annotation to identify the relationship between the epigenetic characteristics and genomic features. The genome sequence contains 52 tRNA genes, 3 rRNA genes and 4,462 protein-coding genes, 3792 assigned as functional or hypothetical proteins in nr database. This Whole Genome Shotgun project was deposited in DDBJ/EMBL/GenBank under the accession JNCS00000000. We performed a Bayesian (Maximum-Likelihood) phylogenetic analysis using 16S rRNA sequence and obtained its relationship to other strains in theNatrinemaandHaloterrigenagenera. We also confirmed the ANI value between every two species ofNatrinemaandHaloterrigenagenera. In conclusion, our analysis furthered our understanding of the extreme-environment adapted strain AJ2Tby characterizing its genome structure, gene content and phylogenetic placement. Our detailed case study will contribute to our overall understanding of whyNatrinemastrains can survive in such a high-altitude salt lake.
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, 310012 People's Republic of China.|College of Life Sciences, Zhejiang University, Hangzhou, 310058 People's Republic of China.
authors.forename: Hong
authors.identifier: 0000-0002-3420-7777
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, 310012 People's Republic of China.
authors.forename: Ying-Yi
authors.initials: YY
authors.lastname: Huo
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058 People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, 310012 People's Republic of China.
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058 People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
keywords: Halophilic archaea; High-altitude; Light-driven pumps; Rhodopsin; Salt lake