title: Construction of a Cordyceps sinensis exopolysaccharide-conjugated selenium nanoparticles and enhancement of their antioxidant activities.
abstract: A Cordyceps sinensis exopolysaccharide (EPS)-conjugated selenium nanoparticles (SeNPs) were successfully constructed through the reduction of SeO32-. The EPS-SeNPs were characterized in terms of formation, morphology, size, Se distribution and phase by UV-vis, FT-IR, transmission electron microscopy (TEM), dynamic light scattering (DLS), energy dispersive X-ray (EDX) and wide angle X-ray diffraction (WAXD) measurements. Results revealed that the SeNPs conjugated to EPS were amorphous and could be well dispersed at a size range of 80-125nm. The interactions between the OH groups of EPS and SeNPs substituted for intermolecular interaction in native EPS to form new CO⋯Se bonds, resulting in good dispersion of SeNPs in the EPS matrix. Besides, the EPS-SeNPs at different Se/P ratios exhibited significant scavenging ability on superoxide anion radical (O2-) and ABTS radical cation (ABTS+) when compared to pure EPS, indicating that the conjugated SeNPs reinforced antioxidant effect of EPS. This work not only provides a simple and efficient way to construct well-dispersed SeNPs in aqueous system, and demonstrates the vital role of the EPS as a biopolymer template for dispersion, stabilization and size control of SeNPs, but also finds the EPS-SeNPs can potentially serve as a good antioxidant towards O2-and ABTS+.
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: hql@mail.hzau.edu.cn.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Han
authors.initials: H
authors.lastname: Guan
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Liu
mesh_terms: D032661:Cordyceps / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D011134:Polysaccharides; D012643:Selenium
keywords: Antioxidant activity; Exopolysaccharide-selenium nanoparticles (EPS-SeNPs); Particle size