title: Conjugated anionic PEG-citrate G2 dendrimer with multi-epitopic HIV-1 vaccine candidate enhance the cellular immune responses in mice.
abstract: Multi-epitope vaccines might cause immunity against multiple antigenic targets. Four immunodominant epitopes of HIV-1 genome were used to construct a polytope vaccine, formulated by dendrimer. Two regimens of polytopes mixture with dendrimer were utilized to immunize BALB/c mice. Adjuvants were also used to boost immune responses. The conjugated polytope could arouse significant cellular immune responses (P < 0.05) and Th1 response showed higher intensity compared to Th2 (P < 0.05). Our study depicted that conjugated dendrimer with multi-epitopic rHIVtop4 would efficiently induce cell-mediated immune responses and might be considered as promising delivery system for vaccines formulation.
authors.affiliation: a Hepatitis and AIDS Department , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Abdoli
authors.affiliation: b Faculty of Basic Sciences , Science and Research Branch, Islamic Azad University , Tehran , Iran.
authors.forename: Nina
authors.initials: N
authors.lastname: Radmehr
authors.affiliation: a Hepatitis and AIDS Department , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Bolhassani
authors.affiliation: b Faculty of Basic Sciences , Science and Research Branch, Islamic Azad University , Tehran , Iran.
authors.forename: Akram
authors.initials: A
authors.lastname: Eidi
authors.affiliation: c Influenza and Other Respiratory Viruses Department , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Parvaneh
authors.identifier: http://orcid.org/0000-0002-8391-9228
authors.initials: P
authors.lastname: Mehrbod
authors.affiliation: a Hepatitis and AIDS Department , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Motevalli
authors.affiliation: d Immunoregulation Research Centre , Shahed University , Tehran , Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Kianmehr
authors.affiliation: e Department of Pilot Biotechnology , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Chiani
authors.affiliation: f Department of Immunology , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Mehdi
authors.identifier: http://orcid.org/0000-0002-3840-7805
authors.initials: M
authors.lastname: Mahdavi
authors.affiliation: g Department of Virology, School of Public Health , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Yazdani
authors.affiliation: h Department of Radiopharmacy, Faculty of Pharmacy , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mehdi Shafiee
authors.initials: MS
authors.lastname: Ardestani
authors.affiliation: i Department of Life Science Engineering, Faculty of New Sciences and Technologies , University of Tehran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Kandi
authors.affiliation: a Hepatitis and AIDS Department , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Mohammad Reza
authors.identifier: http://orcid.org/0000-0003-2186-0774
authors.initials: MR
authors.lastname: Aghasadeghi
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D019343:Citric Acid / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry; D000939:Epitopes / Q000276:immunology*; D005260:Female; D015497:HIV-1 / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000737:chemistry*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D050091:Dendrimers; D000939:Epitopes; D007074:Immunoglobulin G; D014765:Viral Vaccines; D015847:Interleukin-4; D019343:Citric Acid; D011092:Polyethylene Glycols; D007371:Interferon-gamma
keywords: CpG motif; Epitope; HIV; dendrimer; vaccine