title: A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits.
abstract: A millisecond micro-RNA separation of a mixture of total RNA and genomic DNA, extracted from cultured HeLa cells, was successfully achieved using a hybrid structure of nanopillars and nanoslits contained inside a microchannel. The nanopillars, 250-nm in diameter and 100-nm in height, were fabricated with a 750-nm space inside the nanoslits, which were 100-nm in height and 25-μm in width; the nanopillars were then applied as a new sieve matrix. This ultra-fast technique for the separation of miRNA can be an effective pretreatment for semiconductor nanopore DNA sequencing, which has an optimum reading speed of 1 base/ms to obtain effective signal-to-noise ratio and discriminate each base by ion or tunneling current during the passage of nucleic acids.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya 464-8603, Japan.|JST, PRESTO, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya 464-8603, Japan.|JST, PRESTO, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya 464-8603, Japan.
authors.forename: Sakon
authors.initials: S
authors.lastname: Rahong
authors.affiliation: Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka 816-8580, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanagida
authors.affiliation: Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka 816-8580, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kanai
authors.affiliation: Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka 816-8580, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nagashima
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, 8-1Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Tomoji
authors.initials: T
authors.lastname: Kawai
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Nagoya 464-8603, Japan.|Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan.|College of Pharmacy, Kaohsiung Medical University 100, Shih-Chuan 1st Rd., Kaohsiung, 807, Taiwan, R.O.C.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D058608:Nanopores; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D015203:Reproducibility of Results; D012666:Semiconductors; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
chemical_list: D035683:MicroRNAs; C487931:mirnlet7 microRNA, human