title: Colistin Resistance inAcinetobacter baumanniiMDR-ZJ06 Revealed by a Multiomics Approach.
abstract: Acinetobacter baumanniihas emerged as an important opportunistic pathogen due to its ability to acquire resistance to most currently available antibiotics. Colistin is often considered as the last line of therapy for infections caused by multidrug-resistantA. baumannii(MDRAB). However, colistin-resistantA. baumanniistrain has recently been reported. To explore how multiple drug-resistantA. baumanniiresponded to colistin resistance, we compared the genomic, transcriptional and proteomic profile ofA. baumanniiMDR-ZJ06 to the induced colistin-resistant strain ZJ06-200P5-1. Genomic analysis showed thatlpxCwas inactivated by ISAba1insertion, leading to LPS loss. Transcriptional analysis demonstrated that the colistin-resistant strain regulated its metabolism. Proteomic analysis suggested increased expression of the RND efflux pump system and down-regulation of FabZ and β-lactamase. These alterations were believed to be response to LPS loss. In summary, thelpxCmutation not only established colistin resistance but also altered global gene expression.
authors.affiliation: Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityHangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceHangzhou, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Hua
authors.affiliation: Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityHangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceHangzhou, China.
authors.forename: Lilin
authors.initials: L
authors.lastname: Liu
authors.affiliation: The Children's Hospital, College of Medicine, Zhejiang University Hangzhou, China.
authors.forename: Youhong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityHangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceHangzhou, China.
authors.forename: Qiucheng
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Centre of Laboratory Medicine, Zhejiang Provincial People's Hospital Hangzhou, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: Hangzhou First People's Hospital Hangzhou, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityHangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceHangzhou, China.
authors.forename: Keren
authors.initials: K
authors.lastname: Shi
authors.affiliation: Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityHangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceHangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, College of Medicine, Zhejiang University Hangzhou, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityHangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceHangzhou, China; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, China.
authors.forename: Yunsong
authors.initials: Y
authors.lastname: Yu
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016680:Genome, Bacterial; D008070:Lipopolysaccharides / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009154:Mutation; D020543:Proteome / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D020543:Proteome; D003091:Colistin
keywords: Acinetobacter baumannii; colistin; proteome; transcriptome; whole-genome sequencing