title: The oncogenic effects of p53-inducible gene 3 (PIG3) in colon cancer cells.
abstract: The p53-inducible gene 3 (PIG3), initially identified as a gene downstream of p53, plays an important role in the apoptotic process triggered by p53-mediated reactive oxygen species (ROS) production. Recently, several studies have suggested that PIG3 may play a role in various types of cancer. However, the functional significance of PIG3 in cancer remains unclear. Here, we found that PIG3 was highly expressed in human colon cancer cell lines compared to normal colonderived fibroblasts. Therefore, we attempted to elucidate the functional role of PIG3 in colon cancer. PIG3 overexpression increases the colony formation, migration and invasion ability of HCT116 colon cancer cells. Conversely, these tumorigenic abilities were significantly decreased inin vitrostudies with PIG3 knockdown HCT116 cells. PIG3 knockdown also attenuated the growth of mouse xenograft tumors. These results demonstrate that PIG3 is associated with the tumorigenic potential of cancer cells, bothin vitroandin vivo, and could play a key oncogenic role in colon cancer.
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, Gwangju 61452, Korea.; Department of Premedical Sciences, Chosun University School of Medicine, Gwangju 61452, Korea.
authors.forename: Seon-Joo
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, Gwangju 61452, Korea.; Department of Premedical Sciences, Chosun University School of Medicine, Gwangju 61452, Korea.
authors.forename: Hong Beum
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, Gwangju 61452, Korea.
authors.forename: Jeeho
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Hemato-oncology, Chosun University School of Medicine, Gwangju 61452, Korea.
authors.forename: Sanggon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Neurosurgery, Chosun University School of Medicine, Gwangju 61452, Korea.
authors.forename: Seok Won
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, Gwangju 61452, Korea.; Department of Cellular and Molecular Medicine, Chosun University School of Medicine, Gwangju 61452, Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Lee
keywords: Cancer progression; Colon cancer; Oncogenesis; PIG3