title: [Acute hypoxic hypoxia increases lactate concentration in amniotic fluid of rabbits on 27-28th day of pregnancy].
abstract: We evaluated the influence of hypoxic hypoxia on lactate, creatinine and urea concentrations in the amniotic fluid (AF) of rabbits on 27-28th day of pregnancy. Rabbits were randomly sudivided into two groups: experimental (n=9) and control (n=6). Rabbits of experimental groups were placed in a hypoxic chamber containing 10±2% oxygen and 90±2% nitrogen for 1 h and then were euthanized, AF was extracted from the amniotic sacs via disposable syringe. Acute hypoxic hypoxia had no effect on the AF volume, increased (1.4-fold) lactate, (1.3-fold) creatinine and (1.1-fold) urea concentrations in AF. In contrast to animals of the control group, lactate concentration in the groups with hypoxic hypoxia correlated with the creatinine (r=0.71, p<0.0001, n=35) and urea concentrations in the AF (r=0.81, p<0.0001, n=35). These results suggest that acute hypoxic hypoxia in late pregnancy causes changes in the biochemical composition of AF; these changes are characterized by high lactate concentrations, and the fetus and uterus can be the source of increased lactate level in AF.
authors.affiliation: Federal Centre of Traumatology, Orthopedics and Endoprosthesis Replacement, Barnaul, Altai Region, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Popovtseva
authors.affiliation: Altai State Medical University, Barnaul, Altai Region, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Suzopov
authors.affiliation: Altai State Medical University, Barnaul, Altai Region, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Korenovsky
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry / Q000187:drug effects*; D000818:Animals; D003404:Creatinine / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D019344:Lactic Acid / Q000819:agonists / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D010920:Placenta / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits; D014508:Urea / Q000378:metabolism
chemical_list: D019344:Lactic Acid; D014508:Urea; D003404:Creatinine; D010100:Oxygen
keywords: amniotic fluid; hypoxia; lactate