title: Prohibitin promotes androgen receptor activation in ER-positive breast cancer.
abstract: Prohibitin (PHB) is an evolutionarily conserved protein with multiple functions in both normal and cancer cells. Androgen receptor (AR) was reported to act as a different role in the ER-positive and ER-negative breast cancer. However, little is known about the role of PHB and whether PHB could regulate AR expression in the ER-positive breast cancer. Here, we determined the expression and clinical outcomes of PHB in breast cancer samples using 121 breast cancer tissues and published databases, and investigated the role of PHB in breast cancer cell growth, apoptosis and cell cycle arrest in the ER-positive breast cancer cells. We obtained the expression of PHB is significantly low in breast cancer samples, and low PHB expression positively correlated with poor prognosis of breast cancer. We detected that PHB could inhibit breast cancer cell proliferation, change cell cycle distribution and promote cell apoptosis in the ER-positive breast cancer cells. Moreover, we found PHB could significantly increase AR expression in both mRNA and protein levels in the ER-positive breast cancer cells. Additionally, a significant positive correlation between PHB and AR expression was identified in the 121 breast cancer tissues. PHB and AR expression are associated with prognosis in the ER-positive breast cancer patients. Our results indicate that PHB promotes AR activation in ER-positive breast cancer, making PHB and AR potential molecular targets for ER-positive breast cancer therapy.
authors.affiliation: a Department of Medical Oncology , Jinling Hospital, School of Medicine, Southern Medical University , Guangzhou , China.
authors.forename: Pengying
authors.initials: P
authors.lastname: Liu
authors.affiliation: a Department of Medical Oncology , Jinling Hospital, School of Medicine, Southern Medical University , Guangzhou , China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: b Department of Medical Oncology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: a Department of Medical Oncology , Jinling Hospital, School of Medicine, Southern Medical University , Guangzhou , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: b Department of Medical Oncology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.affiliation: b Department of Medical Oncology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.affiliation: b Department of Medical Oncology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: b Department of Medical Oncology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: a Department of Medical Oncology , Jinling Hospital, School of Medicine, Southern Medical University , Guangzhou , China.|b Department of Medical Oncology , Jinling Hospital, Medical School of Nanjing University , Nanjing , China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Guan
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D000091402:Prohibitins; D011944:Receptors, Androgen / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C410798:AR protein, human; C000717328:PHB protein, human; D000091402:Prohibitins; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D012097:Repressor Proteins
keywords: AR; PHB; androgen receptor; breast cancer; prohibitin