title: Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes.
abstract: Cardiotoxicity remains an important concern in drug discovery. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have become an attractive platform to evaluate cardiotoxicity. However, the consistency between human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in prediction of cardiotoxicity has yet to be elucidated.
  
  
  
  Here we screened the toxicities of four representative drugs (E-4031, isoprenaline, quinidine, and haloperidol) using both hESC-CMs and hiPSC-CMs, combined with an impedance-based bioanalytical method.
  
  
  
  It showed that both hESC-CMs and hiPSC-CMs can recapitulate cardiotoxicity and identify the effects of well-characterized compounds.
  
  
  
  The combined platform of hPSC-CMs and an impedance-based bioanalytical method could improve preclinical cardiotoxicity screening, holding great potential for increasing drug development accuracy.
authors.affiliation: China State Institutes of Pharmaceutical Industry National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: China State Institutes of Pharmaceutical Industry National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai, China.
authors.forename: Xijie
authors.initials: X
authors.lastname: Wang
authors.affiliation: China State Institutes of Pharmaceutical Industry National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai, China.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Wang
authors.affiliation: China State Institutes of Pharmaceutical Industry National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Jiaxian
authors.initials: J
authors.lastname: Wang
authors.affiliation: China State Institutes of Pharmaceutical Industry National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai, China. forapplew@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
mesh_terms: D066126:Cardiotoxicity / Q000503:physiopathology*; D002454:Cell Differentiation / Q000235:genetics; D055808:Drug Discovery; D006220:Haloperidol / Q000009:adverse effects; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects*; D007545:Isoproterenol / Q000009:adverse effects; D032383:Myocytes, Cardiac / Q000187:drug effects*; D010880:Piperidines / Q000009:adverse effects; D011725:Pyridines / Q000009:adverse effects; D011802:Quinidine / Q000009:adverse effects
chemical_list: D010880:Piperidines; D011725:Pyridines; C063968:E 4031; D011802:Quinidine; D006220:Haloperidol; D007545:Isoproterenol
keywords: Cardiomyocytes; Cardiotoxicity; Pharmacology; Stem cells