title: Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2nanocomposites to suppress reactive oxygen species.
abstract: Reducing oxidative stress (ROS) have been demonstrated effective for steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH). Selenium (Se) plays an important role in suppressing oxidative stress and has huge potential in ONFH treatments. However the Se has a narrow margin between beneficial and toxic effects which make it hard for therapy use in vivo. In order to make the deficiency up, a control release of Se (Se@SiO2) were realized by nanotechnology modification. Porous Se@SiO2nanocomposites have favorable biocompatibility and can reduced the ROS damage effectively. In vitro, the cck-8 analysis, terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) stain and flow cytometry analysis showed rare negative influence by porous Se@SiO2nanocomposites but significantly protective effect against H2O2by reducing ROS level (detected by DCFH-DA). In vivo, the biosafety of porous Se@SiO2nanocomposites were confirmed by the serum biochemistry, the ROS level in serum were significantly reduced and the curative effect were confirmed by Micro CT scan, serum Elisa assay (inflammatory factors), Western blotting (quantitative measurement of ONFH) and HE staining. It is expected that the porous Se@SiO2nanocomposites may prevent steroid-induced ONFH by reducing oxidative stress.
authors.affiliation: Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin Songjiang Road, Shanghai 201620, P.R. China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Deng
authors.affiliation: Department of Orthopedics, Shanghai Bone Tumor Institute, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China.
authors.forename: Kerun
authors.initials: K
authors.lastname: Niu
authors.affiliation: Department of Orthopedics, Shanghai Bone Tumor Institute, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin Songjiang Road, Shanghai 201620, P.R. China.
authors.forename: Buxiao
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Radiology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine. No. 528, Zhangheng Road, Shanghai 201203, P.R. China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Kang
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, P.R. China.
authors.forename: Xijian
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, No. 1295 Dingxi Road, Shanghai 200050, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin Songjiang Road, Shanghai 201620, P.R. China.
authors.forename: Qiugen
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Shanghai Bone Tumor Institute, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Shanghai 200080, P.R. China.
authors.forename: Chengqing
authors.initials: C
authors.lastname: Yi
authors.affiliation: Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin Songjiang Road, Shanghai 201620, P.R. China.|State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005269:Femur / Q000473:pathology*; D053761:Nanocomposites / Q000008:administration & dosage*; D010020:Osteonecrosis / Q000139:chemically induced* / Q000188:drug therapy*; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D012643:Selenium / Q000008:administration & dosage; D012822:Silicon Dioxide / Q000008:administration & dosage; D013256:Steroids / Q000008:administration & dosage / Q000009:adverse effects*; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D013256:Steroids; D012822:Silicon Dioxide; D012643:Selenium