title: Dual-responsive electrochemical immunosensor for prostate specific antigen detection based on Au-CoS/graphene and CeO2/ionic liquids doped with carboxymethyl chitosan complex.
abstract: Combining dual-signal channels of differential pulse voltammetry (DPV) and amperometric i-t curve, we developed an amplified sandwich-type electrochemical immunosensor for ultrasensitive detection of prostate specific antigen (PSA). Due to the large specific surface area and good adsorption property, 3-aminopropyltriethox functionalized CeO2mesoporous nanoparticles (NH2-M-CeO2) was used for supporting toluidine blue (TB) as the electron transfer mediator and anti-PSA as the signal response. To prevent the leak of TB and facilitate the electron transfer property of NH2-M-CeO2, ionic liquids doped carboxymethyl chitosan (CMC/ILs) were incorporated and TB/M-CeO2/CMC/ILs complex was gotten to act as the label of anti-PSA (Ab2). Meanwhile, Au-CoS/graphene was as the matrix materials modifying the electrode to immobilize anti-PSA (Ab1). Specifically, Au-CoS/graphene cannot produce electrochemical signals through DPV method but can provide an obviously electrochemical signal towards catalysis hydrogen peroxide (H2O2) through amperometric i-t curve method. At the time of detection, with the increase of concentrations of PSA, the DPV signals from TB/M-CeO2/CMC/ILs were increased and the amperometric i-t curve signals from the Au-CoS/graphene were decreased. Under the optimum conditions, the immunosensor demonstrated remarkable analytical performance of a linear range of 0.5pg/mL to 50ng/mL with a detection limit of 0.16pg/mL for quantitative detection of PSA (S/N=3).
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: yongzhang7805@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006108:Graphite; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000302:isolation & purification*
chemical_list: D052578:Ionic Liquids; D006046:Gold; D006108:Graphite; D048271:Chitosan; D017430:Prostate-Specific Antigen
keywords: Au-CoS; Dual-signal channel; Electrochemical immunosensing; Mesoporous CeO(2); PSA; Tumor marker