title: U-Omp19 fromBrucella abortusIs a Useful Adjuvant for Vaccine Formulations againstSalmonellaInfection in Mice.
abstract: Most pathogens infect through mucosal surfaces, and parenteral immunization typically fails to induce effective immune responses at these sites. Development of oral-administered vaccines capable of inducing mucosal as well as systemic immunity while bypassing the issues of antigen degradation and immune tolerance could be crucial for the control of enteropathogens. This study demonstrates that U-Omp19, a bacterial protease inhibitor with immunostimulatory features, coadministered withSalmonellaantigens by the oral route, enhances mucosal and systemic immune responses in mice. U-Omp19 was able to increase antigen-specific production of IFN-γ and IL-17 and mucosal (IgA) antibody response. Finally, oral vaccination with U-Omp19 plusSalmonellaantigens conferred protection against virulent challenge withSalmonellaTyphimurium, with a significant reduction in bacterial loads. These findings prove the efficacy of this novel adjuvant in theSalmonellainfection model and support the potential of U-Omp19 as a suitable adjuvant in oral vaccine formulations against mucosal pathogens requiring T helper (Th)1-Th17 protective immune responses.
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Gabriela S
authors.initials: GS
authors.lastname: Risso
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Marianela V
authors.initials: MV
authors.lastname: Carabajal
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Bruno
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Andrés E
authors.initials: AE
authors.lastname: Ibañez
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Lorena M
authors.initials: LM
authors.lastname: Coria
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Karina A
authors.initials: KA
authors.lastname: Pasquevich
authors.affiliation: Center for Comparative Medicine (CCM), Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California Davis , Davis, CA , USA.
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Center for Comparative Medicine (CCM), Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California Davis , Davis, CA , USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: McSorley
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Briones
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires , Argentina.
authors.forename: Juliana
authors.initials: J
authors.lastname: Cassataro
keywords: Salmonella Typhimurium; T helper 1; T helper 17; adjuvant; vaccination