title: Antiviral Resistance Protein Tm-22Functions on the Plasma Membrane.
abstract: The tomatoTobacco mosaic virus resistance-22(Tm-22) gene encodes a coiled-coil-nucleotide binding site-Leu-rich repeat protein lacking a conventional plasma membrane (PM) localization motif. Tm-22confers plant extreme resistance against tobamoviruses includingTobacco mosaic virus(TMV) by recognizing the avirulence (Avr) viral movement protein (MP). However, the subcellular compartment where Tm-22functions is unclear. Here, we demonstrate that Tm-22interacts with TMV MP to form a protein complex at the PM We show that both inactive and active Tm-22proteins are localized to the PM When restricted to PM by fusing Tm-22to theS-acylated PM association motif, the Tm-22fusion protein can still induce a hypersensitive response cell death, consistent with its activation at the PM Through analyses of viral MP mutants, we find that the plasmodesmata (PD) localization of the Avr protein MP is not required for Tm-22function. These results suggest that Tm-22-mediated resistance takes place on PM without requirement of its Avr protein to be located to PD.
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Tianyuan
authors.initials: T
authors.lastname: Chen
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Niu
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Junzhu
authors.identifier: 0000-0003-1918-6573
authors.initials: J
authors.lastname: Wang
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Lichao
authors.initials: L
authors.lastname: Qian
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.).|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.).
authors.forename: Yiguo
authors.identifier: 0000-0002-3352-9686
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (T.C., D.L., X.N., J.W., L.Q., L.H., N.L., J.Z., Y.L.); yuleliu@mail.tsinghua.edu.cn.|State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China (J.Z.); and yuleliu@mail.tsinghua.edu.cn.|Research Centre for Plant RNA Signaling, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China (Y.H.) yuleliu@mail.tsinghua.edu.cn.
authors.forename: Yule
authors.identifier: 0000-0002-4423-6045
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002462:Cell Membrane / Q000378:metabolism* / Q000821:virology; D060467:Disease Resistance / Q000235:genetics; D015151:Immunoblotting; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism / Q000821:virology; D018613:Microscopy, Confocal; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D031425:Plasmodesmata / Q000378:metabolism* / Q000821:virology; D011485:Protein Binding; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D010940:Plant Proteins; D053830:Plant Viral Movement Proteins