title: Ultrasonic disruption of Pseudomonas putida for the release of arginine deiminase: Kinetics and predictive models.
abstract: The responses of the ultrasound-mediated disruption of Pseudomonas putida KT2440 were modelled as the function of biomass concentration in the cell suspension; the treatment time of sonication; the duty cycle and the acoustic power of the sonicator. For the experimental data, the response surface (RSM), the artificial neural network (ANN) and the support vector machine (SVM) models were compared for their ability to predict the performance parameters. The satisfactory prediction of the unseen data of the responses implied the proficient generalization capabilities of ANN. The extent of the cell disruption was mainly dependent on the acoustic power and the biomass concentration. The cellmass concentration in the slurry most strongly influenced the ADI and total protein release. Nearly 28U/mL ADI was released when a biomass concentration of 300g/L was sonicated for 6min with an acoustic power of 187.5W at 40% duty cycle. Cell disruption obeyed first-order kinetics.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062, Punjab, India. Electronic address: mahi1709@gmail.com.
authors.forename: Mahesh D
authors.initials: MD
authors.lastname: Patil
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062, Punjab, India. Electronic address: manojdev21@gmail.com.
authors.forename: Manoj J
authors.initials: MJ
authors.lastname: Dev
authors.affiliation: Department of Pharmacoinformatics, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062, Punjab, India. Electronic address: sujit.tangadpalliwar@gmail.com.
authors.forename: Sujit
authors.initials: S
authors.lastname: Tangadpalliwar
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062, Punjab, India. Electronic address: gopal87patel@gmail.com.
authors.forename: Gopal
authors.initials: G
authors.lastname: Patel
authors.affiliation: Department of Pharmacoinformatics, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062, Punjab, India. Electronic address: prabhagarg@niper.ac.in.
authors.forename: Prabha
authors.initials: P
authors.lastname: Garg
authors.affiliation: School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand. Electronic address: Y.Chisti@massey.ac.nz.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Chisti
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062, Punjab, India. Electronic address: ucbanerjee@niper.ac.in.
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
mesh_terms: D018533:Biomass; D007700:Kinetics; D008962:Models, Theoretical; D016571:Neural Networks, Computer; D016958:Pseudomonas putida / Q000378:metabolism*
keywords: Arginine deiminase; Artificial neural network; Pseudomonas putida; Support vector machine; Ultrasonication