title: The proteomic profile of the acquired enamel pellicle according to its location in the dental arches.
abstract: OBJECTIVE
  This study evaluated the variation in the protein profile of the acquired enamel pellicle (AEP) formed in vivo according to its location in the dental arches.
  
  
  METHODS
  The AEP was formed for 120min in 9 volunteers. Pellicle formed at upper+lower anterior facial (ULAFa; teeth 13-23 and 33-43), upper anterior palatal (UAPa; teeth 13-23), lower anterior lingual (LALi; teeth 33-43), upper+lower posterior facial (ULPFa; teeth 14-17 24-27, 34-37 and 44-47), upper posterior palatal (UPPa; teeth 14-17 and 24-27) and lower posterior lingual (LPLi; teeth 34-37 and 44-47) regions were collected separately and processed for analysis by label-free LC-ESI-MS/MS.
  
  
  RESULTS
  Three-hundred sixty three proteins were identified in total, twenty-five being common to all the locations, such as Protein S100-A8, Lysozyme C, Lactoferrin, Statherin, Ig alpha-2, ALB protein, Myeloperoxidase and SMR3B. Many proteins were found exclusively in the AEP collected from one of the regions (46-UAPa, 33-LALi, 59-ULAFa, 31-ULPFa, 44-LPLi and 39-UPPa).
  
  
  CONCLUSIONS
  The protein composition of the AEP varied according to its location in the dental arches. These results provide important insights for understanding the differential protective roles of the AEP as a function of its location in the dental arches.
authors.affiliation: Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: talitaventura@usp.br.
authors.forename: Talita Mendes da Silva
authors.initials: TMDS
authors.lastname: Ventura
authors.affiliation: Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: luizacassiano@hotmail.com.
authors.forename: Luiza de Paula Silva
authors.initials: LPS
authors.lastname: Cassiano
authors.affiliation: Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: cintiaours@gmail.com.
authors.forename: Cíntia Maria de
authors.initials: CM
authors.lastname: Souza E Silva
authors.affiliation: Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: even.akemi@gmail.com.
authors.forename: Even Akemi
authors.initials: EA
authors.lastname: Taira
authors.affiliation: Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: a.lima82@yahoo.com.br.
authors.forename: Aline de Lima
authors.initials: AL
authors.lastname: Leite
authors.affiliation: Department of Pediatric Dentistry, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: danirios@usp.br.
authors.forename: Daniela
authors.initials: D
authors.lastname: Rios
authors.affiliation: Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: mbuzalaf@fob.usp.br.
authors.forename: Marília Afonso Rabelo
authors.initials: MAR
authors.lastname: Buzalaf
mesh_terms: D002135:Calcium-Binding Proteins / Q000378:metabolism; D015891:Cystatins; D003724:Dental Arch / Q000378:metabolism*; D044622:Dental Pellicle / Q000378:metabolism*; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009195:Peroxidase; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D009418:S100 Proteins / Q000378:metabolism; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D012471:Salivary Proteins and Peptides; D000075462:Serum Albumin, Human / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods; D014838:Volunteers; D000096985:Calponins
chemical_list: D002135:Calcium-Binding Proteins; D015891:Cystatins; D007781:Lactoferrin; D008840:Microfilament Proteins; D009113:Muramidase; D009195:Peroxidase; D020543:Proteome; D009418:S100 Proteins; D012471:Salivary Proteins and Peptides; D000075462:Serum Albumin, Human; D000096985:Calponins; C422455:ALB protein, human; C110294:S100A1 protein; C018149:STATH protein, human; C514728:lysozyme C, human
keywords: Acquired pellicle; Enamel; Proteomics; Saliva