title: Effects of Selenium Nanoparticles on Reproductive Performance of Male Sprague-Dawley Rats at Supranutritional and Nonlethal Levels.
abstract: This research investigated the influence of selenium nanoparticles (SeNPs) on the reproductive performance of male Sprague-Dawley (SD) rats. A suspension of SeNPs was consecutively administered by oral gavage for 2 weeks at supranutritional (0.2, 0.4, or 0.8 mg Se/kg bw) and nonlethal (2.0, 4.0, or 8.0 mg Se/kg bw) levels to male SD rats. The normal control (NC) rats were exposed to physiological saline alone. Biochemical parameters, sperm motility, gene expression of GPx1 and GPx4, and histopathological evaluation of male spermary were measured in this work. The supranutritional doses could promote the sperm motility (P < 0.001) and movement parameters (P < 0.05). The nonlethal levels of 4.0 and 8.0 mg Se/kg bw reduced the testis weight (P < 0.001), sperm concentration, and motility (P < 0.05), and also caused histopathological injury of testis and epididymis tissues to various degrees. The content of testosterone in serum was increased in the 0.8 group (P < 0.05) and decreased in the 4.0 (P < 0.01) and 8.0 mg Se/kg bw groups (P < 0.001), respectively. No significant effects were observed on antioxidant enzyme activities and mRNA concentration of GPx in the supranutritional dose group, and nonlethal levels were also not observed. In conclusion, SeNPs in the supranutritional dose has a positive effect on the reproductive function of male SD rats and has damaging effect higher than 4.0 mg Se/kg bw.
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.
authors.forename: Lujie
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Animal Science, Jiangxi Biotech Vocational College, 608 Nanlian Road, Nanchang, 330200, Jiangxi, People's Republic of China.
authors.forename: Yudan
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Tao
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.
authors.forename: Zixun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China. wangmq@zju.edu.cn.
authors.forename: Minqi
authors.initials: M
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage*; D009929:Organ Size / Q000187:drug effects; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000008:administration & dosage* / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D004798:Enzymes; D013739:Testosterone; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; C523860:glutathione peroxidase 4, rat; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat
keywords: Glutathione peroxidase; Reproduction; Selenium nanoparticles; Testis