title: Comprehensive proteomic analysis of Penicillium verrucosum.
abstract: Mass spectrometric identification of proteins in species lacking validated sequence information is a major problem in veterinary science. In the present study, we used ochratoxin A producing Penicillium verrucosum to identify and quantitatively analyze proteins of an organism with yet no protein information available. The work presented here aimed to provide a comprehensive protein identification of P. verrucosum using shotgun proteomics. We were able to identify 3631 proteins in an "ab initio" translated database from DNA sequences of P. verrucosum. Additionally, a sequential window acquisition of all theoretical fragment-ion spectra analysis was done to find differentially regulated proteins at two different time points of the growth curve. We compared the proteins at the beginning (day 3) and at the end of the log phase (day 12).
authors.affiliation: VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Nöbauer
authors.affiliation: VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Hummel
authors.affiliation: Department for Biomedical Sciences, Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria.|Interuniversity Department for Agrobiotechnology (IFA Tulln), Institute of Biotechnology in Animal Production, University of Natural Resources and Applied Life Sciences, Vienna, Tulln, Austria.
authors.forename: Corina
authors.initials: C
authors.lastname: Mayrhofer
authors.affiliation: Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Ahrens
authors.affiliation: VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Francis M C
authors.initials: FMC
authors.lastname: Setyabudi
authors.affiliation: Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Fruits and Vegetables, Karlsruhe, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Schmidt-Heydt
authors.affiliation: Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Eisenacher
authors.affiliation: VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Razzazi-Fazeli
mesh_terms: D030562:Databases, Protein; D005656:Fungal Proteins / Q000378:metabolism*; D009793:Ochratoxins / Q000032:analysis*; D010407:Penicillium / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D005656:Fungal Proteins; D009793:Ochratoxins; D020543:Proteome; C025589:ochratoxin A
keywords: Fungal proteomics; Penicillium verrucosum; SWATH; Shotgun proteomics