title: CTCF participates in DNA damage response via poly(ADP-ribosyl)ation.
abstract: CCCTC-binding factor (CTCF) plays an essential role in regulating the structure of chromatin by binding DNA strands for defining the boundary between active and heterochromatic DNA. However, the role of CTCF in DNA damage response remains elusive. Here, we show that CTCF is quickly recruited to the sites of DNA damage. The fast recruitment is mediated by the zinc finger domain and poly (ADP-ribose) (PAR). Further analyses show that only three zinc finger motifs are essential for PAR recognition. Moreover, CTCF-deficient cells are hypersensitive to genotoxic stress such as ionizing radiation (IR). Taken together, these results suggest that CTCF participate in DNA damage response via poly(ADP-ribosylation).
authors.affiliation: Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope, 1500 E. Duarte Rd, Duarte, California, 91010, USA.|Department of Biochemistry and Molecular Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.
authors.forename: Deqiang
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope, 1500 E. Duarte Rd, Duarte, California, 91010, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope, 1500 E. Duarte Rd, Duarte, California, 91010, USA.|Department of Cell Biology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Jiazhong
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope, 1500 E. Duarte Rd, Duarte, California, 91010, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope, 1500 E. Duarte Rd, Duarte, California, 91010, USA.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
mesh_terms: D001665:Binding Sites; D000076246:CCCTC-Binding Factor / Q000378:metabolism*; D002460:Cell Line; D004249:DNA Damage; D006801:Humans; D000074747:Poly ADP Ribosylation; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011485:Protein Binding; D016335:Zinc Fingers
chemical_list: D000076246:CCCTC-Binding Factor; D011064:Poly Adenosine Diphosphate Ribose; D011065:Poly(ADP-ribose) Polymerases