title: Role of the Lateral Habenula in Pain-Associated Depression.
abstract: Patients with chronic pain have significantly higher incidences of depression and anxiety than the average person. However, the mechanism underlying this link has not been elucidated in terms of how chronic pain causes significant mood changes and further develops into severe anxiety or depression. The serotonergic system in the raphe nuclei is an important component in both pain processing and the pathogenesis of depression. Since the lateral habenular nucleus (LHb) controls the raphe nuclei, it may participate in the regulation of pain-associated depression. Thus, the aim of the current study was to investigate the role of the LHb in this pathophysiological process. We used chronic constriction injury (CCI) of the sciatic nerve in rats as a model for neuropathic pain and assessed the changes potentially related to the mood disorders. The forced swim test (FST) and sucrose preference test (SPT) were performed to determine the behavioral changes 28 days after pain surgery. Expression of β calmodulin-dependent protein kinase type II (βCaMKII) in the LHb, cytochrome-c oxidase (COX) activity in the LHb and dorsal raphe nucleus (DRN) and serotonin (5-HT) levels in the DRN were measured. We found an increasing in LHb activity and βCaMKII expression, and a decrease in neuronal activity in the DRN and 5-hydroxyindoleacetic acid (5-HIAA)/5-HT ratios in the CCI rats. These effects were accompanied by the depression-like behaviors. Lesions in the LHb improved the pain threshold and depression-like behavior in the rats. These results suggest that the LHb may play a role in pain-associated depression by affecting the activity of 5-HT neurons in the DRN. Furthermore, we showed that increases in the LHb-DRN pathway activity were a common neurobiological mechanisms for pain and depression, which may explain the coexistence of pain and depression.
authors.affiliation: Department of Physiology, College of Basic Medical Sciences, Jilin UniversityChangchun, China; Department of Anesthesia, Neuroscience Research Center, First Hospital of Jilin UniversityChangchun, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Physiology, College of Basic Medical Sciences, Jilin University Changchun, China.
authors.forename: Yumeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesia, Neuroscience Research Center, First Hospital of Jilin University Changchun, China.
authors.forename: Chengluan
authors.initials: C
authors.lastname: Xuan
authors.affiliation: Department of Physiology, College of Basic Medical Sciences, Jilin University Changchun, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Physiology, College of Basic Medical Sciences, Jilin University Changchun, China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Piao
authors.affiliation: Department of Physiology, College of Basic Medical Sciences, Jilin University Changchun, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Physiology, College of Basic Medical Sciences, Jilin UniversityChangchun, China; Department of Anesthesia, Neuroscience Research Center, First Hospital of Jilin UniversityChangchun, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
keywords: chronic constriction injury; chronic neuropathic pain; depression; dorsal raphe nucleus; lateral habenula; serotonin