title: The first description of a hormone-sensitive lipase from a basidiomycete: Structural insights and biochemical characterization revealed Bjerkandera adusta BaEstB as a novel esterase.
abstract: The heterologous expression and characterization of a Hormone-Sensitive Lipases (HSL) esterase (BaEstB) from the Basidiomycete fungus Bjerkandera adusta is reported for the first time. According to structural analysis, amino acid similarities and conservation of particular motifs, it was established that this enzyme belongs to the (HSL) family. The cDNA sequence consisted of 969 nucleotides, while the gene comprised 1133, including three introns of 57, 50, and 57 nucleotides. Through three-dimensional modeling and phylogenetic analysis, we conclude that BaEstB is an ortholog of the previously described RmEstB-HSL from the phylogenetically distant fungus Rhizomucor miehei. The purified BaEstB was characterized in terms of its specificity for the hydrolysis of different acyl substrates confirming its low lipolytic activity and a noticeable esterase activity. The biochemical characterization of BaEstB, the DLS analysis and the kinetic parameters determination revealed this enzyme as a true esterase, preferentially found in a dimeric state, displaying activity under alkaline conditions and relative low temperature (pH = 10, 20°C). Our data suggest that BaEstB is more active on substrates with short acyl chains and bulky aromatic moieties. Phylogenetic data allow us to suggest that a number of fungal hypothetical proteins could belong to the HSL family.
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: María Del Rayo
authors.initials: MDR
authors.lastname: Sánchez-Carbente
authors.affiliation: Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Ramón Alberto
authors.initials: RA
authors.lastname: Batista-García
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.|Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Ayixón
authors.initials: A
authors.lastname: Sánchez-Reyes
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Angela
authors.initials: A
authors.lastname: Escudero-Garcia
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Catalina
authors.initials: C
authors.lastname: Morales-Herrera
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.|Departamento de Biología, Facultad de Ciencias, Universidad Antonio Nariño, Bogota, Colombia.
authors.forename: Laura I
authors.initials: LI
authors.lastname: Cuervo-Soto
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Leidys
authors.initials: L
authors.lastname: French-Pacheco
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Arline
authors.initials: A
authors.lastname: Fernández-Silva
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Amero
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
authors.forename: Edmundo
authors.initials: E
authors.lastname: Castillo
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Jorge Luis
authors.identifier: 0000-0002-1860-8175
authors.initials: JL
authors.lastname: Folch-Mallol
mesh_terms: D016000:Cluster Analysis; D055453:Coriolaceae / Q000201:enzymology* / Q000235:genetics*; D018076:DNA, Complementary; D007438:Introns; D007700:Kinetics; D008958:Models, Molecular; D010802:Phylogeny; D011487:Protein Conformation; D055503:Protein Multimerization; D020103:Rhizomucor / Q000201:enzymology / Q000235:genetics; D017385:Sequence Homology; D002787:Sterol Esterase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D018076:DNA, Complementary; D002787:Sterol Esterase
keywords: 
  Bjerkandera adusta
  ; Hormone-Sensitive Lipase; ergosterol esters; esterase