title: Synergetic effect of topological cue and periodic mechanical tension-stress on osteogenic differentiation of rat bone mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are able to self-renew and differentiate into tissues of mesenchymal origin, making them to be significant for cell-based therapies, such as metabolic bone diseases and bone repair. Regulating the differentiation of MSCs is significant for bone regeneration. Electrospun fibers mimicking natural extracellular matrix (ECM), is an effective artificial ECM to regulate the behaviors and fates of MSCs. The aligned electrospun fibers can modulate polar cell pattern of bone mesenchymal stem cells, which leads to more obvious osteogenic differentiation. Apart from the topographic effect of electrospun fibers, mechanical cues can also intervene the cell behaviors. In this study, the osteogenic differentiation of rat bone mesenchymal stem cells was evaluated, which were cultured on aligned/random electrospun fiber mats materials under mechanical tension intervention. Scanning electron microscope and immune-fluorescent staining were used to directly observe the polarity changing of cellular morphology and cytoskeleton. The results proved that aligned electrospun fibers could be more conducive to promote osteogenic differentiation of rat bone mesenchymal stem cells and this promotion of osteogenic differentiation was enhanced by tension intervention. These results were correlated to the quantitative real-time PCR assay. In general, culturing rat bone mesenchymal stem cells on electrospun fibers under the intervention of mechanical tension is an effective way to mimic a more real cellular microenvironment.
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Advanced Technologies of Material, Minister of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
authors.forename: Huanzhong
authors.initials: H
authors.lastname: Ji
authors.affiliation: Key Laboratory of Advanced Technologies of Material, Minister of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China. Electronic address: luoen521125@sina.com.
authors.forename: En
authors.initials: E
authors.lastname: Luo
authors.affiliation: Key Laboratory of Advanced Technologies of Material, Minister of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address: shaobingzhou@hotmail.com.
authors.forename: Shaobing
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics; D001861:Bone Regeneration; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D003599:Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D011091:Polyesters / Q000737:chemistry; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D013314:Stress, Mechanical; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D011091:Polyesters; C493717:Runx2 protein, rat; C016240:polycaprolactone
keywords: Cell polarization; Cytoskeleton; Electrospun fiber; Mechanical tension; Osteogenic differentiation