title: Maternal genetic backgrounds contribute to the genetic susceptibility of tongue cancer patients in Hunan, central of China.
abstract: Mitochondrial DNA (mtDNA) mutations played crucial roles on affecting the susceptibility to cancer. In this study, to investigate whether mitochondrial DNA mutations contributed to the genetic susceptibility of Chinese tongue cancer patients, mtDNA control regions of 105 Chinese tongue cancer patients were amplified and sequenced, the mutations were recorded by comparing with the revised Cambridge Reference Sequence (rCRS), which were attributed to certain mtDNA haplogroups based on the specific variations motif of each patients. The Miao Chinese group (a Chinese ethnic minority) from surrounding region has no essential difference with tongue cancer group, which was taken as the matched control group with principal component analysis by taking the haplogroups frequency of 105 tongue cancer individuals and 354 healthy individuals of eight groups from the similar geographic regions as input factors. This was supported by the smallest genetic distance between tongue cancer and Miao_2 groups. Further, the statistical analysis based on mtDNA variations of hypervariable sequence I (HVSI) indicated that 13 variations including 16,124, 16,148, 16,182C, 16,183C, 16,227, 16,266A, 16,249, 16,272, 16,291, 16,327, 16,335, 16,497, and 16,519 have significant differences between tongue cancer group and matched control group. Comparison of mtDNA haplogroups between tongue cancer and control groups indicated that mtDNA haplogroups C, F2*, and M10 have significant differences. It's worth noting that 16,327 and 16,291 was the defining variation of haplogroups C and F2*, respectively. Our results suggested that mitochondrial DNA may play a crucial role for the maternal genetic susceptibility of tongue cancer patients from Hunan, central of China.
authors.affiliation: a Key Laboratory of Genetics and Birth Health of Hunan Province , Family Planning Institute of Hunan Province , Changsha , Hunan Province , China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhou
authors.affiliation: a Key Laboratory of Genetics and Birth Health of Hunan Province , Family Planning Institute of Hunan Province , Changsha , Hunan Province , China.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Shu
authors.affiliation: b Department of Head & Neck (Oncoplastic Surgery) , Hunan Cancer Hospital , Changsha , Hunan Province , China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dai
authors.affiliation: b Department of Head & Neck (Oncoplastic Surgery) , Hunan Cancer Hospital , Changsha , Hunan Province , China.
authors.forename: Hong-Chao
authors.initials: HC
authors.lastname: Li
authors.affiliation: c Department of Reproduction and Genetics , The First Affiliated Hospital of Kunming Medical University , Kunming , Yunnan Province , China.|d Department of Reproduction and Genetics, The First People's Hospital of Yunnan Province , Kunming , Yunnan Province , China.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.affiliation: c Department of Reproduction and Genetics , The First Affiliated Hospital of Kunming Medical University , Kunming , Yunnan Province , China.
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Genetics and Birth Health of Hunan Province , Family Planning Institute of Hunan Province , Changsha , Hunan Province , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ni
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D000072741:Maternal Inheritance; D008875:Middle Aged; D011110:Polymorphism, Genetic; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D014062:Tongue Neoplasms / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial
keywords: Tongue cancer; mtDNA; susceptibility