title: A novel chitosan hydrogel membrane by an improved electrophoretic deposition and its characteristics in vitro and in vivo.
abstract: Here, we report a novel chitosan hydrogel membrane (CHM) created by an improved electrophoretic deposition. Unlike a traditional CHM by electrophoretic deposition, the CHM was formed on a nanoporous film as a barrier using a homemade device at a high DC voltage (60 VDC). The CHM maximum recovery of 81.7% could be achieved after 1h of electrophoretic deposition. The transparent CHM with an elongation of 42.46% and swelling index of 538.86% was a mixture of type I and type II crystal structures. SEM revealed that the CHM had an irregular net structure. The CHM was sufficient for L-929 mouse fibroblast cell adhesion and growth. To demonstrate immunocompatibility with host tissues, H&E and TGF-β1were observed and the expressions of TNF-α and NF-κB were measured up to 3weeks post-implantation. Although these scaffolds demonstrated an initial pro-inflammatory response, the amount of inflammatory cells and the expressions of TGF-β1returned to baseline control values at 3weeks. The expressions of TNF-α and NF-κB had no significant difference between the experimental and control groups. Animal experiments showed that the CHM did not incite serious inflammatory reactions. Thus, the CHM is a promising medical biomaterial candidate for loading appropriate cells for use as artificial skin or in transplantation.
authors.affiliation: Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, National Engineering Laboratory for Modern Silk, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Li
authors.affiliation: Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, National Engineering Laboratory for Modern Silk, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, National Engineering Laboratory for Modern Silk, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China. Electronic address: sericult@suda.edu.cn.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008855:Microscopy, Electron, Scanning; D016328:NF-kappa B / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D053773:Transforming Growth Factor beta1 / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D016328:NF-kappa B; D053773:Transforming Growth Factor beta1; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan
keywords: Chitosan; Electrophoretic deposition; Hydrogel membrane; Medical biomaterials