title: Knockdown of PARP6 or survivin promotes cell apoptosis and inhibits cell invasion of colorectal adenocarcinoma cells.
abstract: Colorectal adenocarcinoma is the third most common cancer worldwide. PARP6, a novel member of the poly(ADP-ribose) polymerases (PARPs) and survivin, a member of the family of inhibitor of apoptosis (IAP) proteins are associated with a poor prognosis in various types of cancers. However, limited evidence exists regarding the interaction between PARP6 and survivin in colorectal adenocarcinoma. In the present study, we used the paired samples of 20 patients with colorectal adenocarcinoma to detect the expression of PARP6 and survivin in both tumor and adjacent normal colorectal mucosa. Their interaction and roles in cell viability, cell cycle, cell apoptosis and cell invasion were further investigated. Our results showed that both PARP6 and survivin exhibited higher expression in colorectal adenocarcinoma tissues and SW620 cells when compared with levels in adjacent non-tumor tissues and a normal colon cell line FHC. Co-immunoprecipitation assay showed that a significant correlation existed between PARP6 and survivin. We also showed that sole treatment of PARP6 siRNA or survivin siRNA partially inhibited the cell survival and invasion, induced cell G0/G1 arrest, and cell apoptosis at the early and late stages. The combined treatment of PARP6 siRNA and survivin siRNA suppressed the cell survival and cell invasion, further induced cell cycle phase G0/G1 arrest, and cell apoptosis at the early and late stages. Taken together, knockdown of PARP6 or survivin promotes cell apoptosis and inhibits the cell invasion of colorectal adenocarcinoma cells. A significant correlation exists between PARP6 and survivin, and both are promising targets for the development of new strategies for the diagnosis and treatment of advanced or metastatic colorectal adenocarcinoma.
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University, Lingui, Guilin, Guangxi Zhuang Autonomous Region, Guangxi 541100, P.R. China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University, Lingui, Guilin, Guangxi Zhuang Autonomous Region, Guangxi 541100, P.R. China.
authors.forename: Shengguo
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University, Lingui, Guilin, Guangxi Zhuang Autonomous Region, Guangxi 541100, P.R. China.
authors.forename: Xishun
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University, Lingui, Guilin, Guangxi Zhuang Autonomous Region, Guangxi 541100, P.R. China.
authors.forename: Zhike
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University, Lingui, Guilin, Guangxi Zhuang Autonomous Region, Guangxi 541100, P.R. China.
authors.forename: Xiangkai
authors.initials: X
authors.lastname: Long
authors.affiliation: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University, Lingui, Guilin, Guangxi Zhuang Autonomous Region, Guangxi 541100, P.R. China.
authors.forename: Xijia
authors.initials: X
authors.lastname: Zhu
mesh_terms: D036002:ADP Ribose Transferases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques / Q000379:methods*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000494:pharmacology; D000077022:Survivin; D015854:Up-Regulation
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin; D036002:ADP Ribose Transferases; C579799:PARP6 protein, human