title: Possible correlation between levansucrase production and probiotic activity of Bacillus sp. isolated from honey and honey bee.
abstract: Five bacterial isolates from honey and bee gut were selected based on their high levansucrase activity and levan yield which were strongly positively correlated. All isolates showed good tolerance to temperature up to 70 °C, to NaCl up to 3 M and to 0.1% H2O2. They maintained over 59 and 64% survival at pH 9.0 and 2.0 respectively, but showed varying tolerance to 0.1% bile salts and pancreatic enzymes. Most isolates were susceptible to widely used antibiotics, but demonstrated diverse antimicrobial activity. Non hemolytic isolates were identified on the basis of 16S rRNA sequencing as Bacillus subtilis HMNig-2 and B. subtilis MENO2 with 97% homology. They exhibited promising probiotic characteristics and achieved highest levansucrase activity of 94.1 and 81.5 U/mL respectively. Both exhibited highest biofilm formation ability in static microtiter plate assay. Also, they achieved 34 and 26% adhesion respectively to Caco-2cells and had highest free radical scavenging activity of 30.8 and 26.2% respectively. The levans of the two isolates showed good antimicrobial activity against some pathogens and exhibited positive prebiotic effect (prebiotic index >1) with Lactobacillus casei and Lactobacillus reuteri. Results suggest a correlation between levansucrase production, levan yield and pre-probiotic activities of the studied strains.
authors.affiliation: Pharmaceutical Industries Division, Chemistry of Natural and Microbial Products Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, P.O. Box 12622, Giza, Egypt.
authors.forename: Abdelhamid A
authors.initials: AA
authors.lastname: Hamdy
authors.affiliation: Pharmaceutical Industries Division, Chemistry of Natural and Microbial Products Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, P.O. Box 12622, Giza, Egypt.
authors.forename: Nouran A
authors.initials: NA
authors.lastname: Elattal
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Magdy A
authors.initials: MA
authors.lastname: Amin
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Amal E
authors.initials: AE
authors.lastname: Ali
authors.affiliation: Pharmaceutical Industries Division, Chemistry of Natural and Microbial Products Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, P.O. Box 12622, Giza, Egypt.
authors.forename: Nahla M
authors.initials: NM
authors.lastname: Mansour
authors.affiliation: Pharmaceutical Industries Division, Chemistry of Natural and Microbial Products Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, P.O. Box 12622, Giza, Egypt.
authors.forename: Ghada E A
authors.initials: GE
authors.lastname: Awad
authors.affiliation: Pharmaceutical Industries Division, Chemistry of Natural and Microbial Products Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, P.O. Box 12622, Giza, Egypt.
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Awad
authors.affiliation: Pharmaceutical Industries Division, Chemistry of Natural and Microbial Products Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, P.O. Box 12622, Giza, Egypt. mona_esawy@hotmail.com.
authors.forename: Mona A
authors.initials: MA
authors.lastname: Esawy
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification* / Q000502:physiology; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism; D001516:Bees / Q000382:microbiology*; D018938:Caco-2 Cells; D006602:Hexosyltransferases / Q000378:metabolism*; D006722:Honey / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D019936:Probiotics / Q000494:pharmacology; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017423:Sequence Analysis, RNA; D000071436:Thermotolerance
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006602:Hexosyltransferases; C021513:levansucrase
keywords: Honey isolates; Levan; Levansucrase; Prebiotic; Probiotic bacteria