title: Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer.
abstract: Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer cell line TPC-1. The in vitro cytotoxicity of harmine evaluated by XTT assay indicated that harmine suppressed the proliferation of TPC-1 cells in a dose- and time-dependent manner. Moreover, harmine dose-dependently induced apoptosis of TPC-1 cells through regulating the ratio of Bcl-2/Bax. The colony forming ability of TPC-1 cells was also time-dependently inhibited by harmine. The inhibitory effects of harmine on migration and invasion of TPC-1 cells were studied by wound scratching and Transwell assays. In vivo evaluation showed that harmine effectively inhibited the growth of thyroid cancer in a dose-dependent manner in nude mice. Therefore, harmine might be a promising herbal medicine in the therapy of thyroid cancer and further efforts are needed to explore this therapeutic strategy.
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China; Department of Endocrinology, Yancheng Third Hospital, The Affiliated Hospital of Southeast University Medical College, Yancheng, China.
authors.forename: Shu
authors.identifier: 0000-0002-5288-7331
authors.initials: S
authors.lastname: Ruan
authors.affiliation: Department of Neurosurgery, Yancheng City No. 1 People's Hospital, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng, China.
authors.forename: Feng
authors.identifier: 0000-0001-7916-4566
authors.initials: F
authors.lastname: Jia
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Jianbo
authors.identifier: 0000-0002-2188-6205
authors.initials: J
authors.lastname: Li