title: Assembly of Dynamic P450-Mediated Metabolons-Order Versus Chaos.
abstract: OBJECTIVE
  We provide an overview of the current knowledge on cytochrome P450-mediated metabolism organized as metabolons and factors that facilitate their stabilization. Essential parameters will be discussed including those that are commonly disregarded using the dhurrin metabolon fromSorghum bicoloras a case study.
  
  
  RESULTS
  Sessile plants control their metabolism to prioritize their resources between growth and development, or defense. This requires fine-tuned complex dynamic regulation of the metabolic networks involved. Within the recent years, numerous studies point to the formation of dynamic metabolons playing a major role in controlling the metabolic fluxes within such networks.
  
  
  CONCLUSIONS
  We propose that P450s and their partners interact and associate dynamically with POR, which acts as a charging station possibly in concert with Cytb5. Solvent environment, lipid composition, and non-catalytic proteins guide metabolon formation and thereby activity, which have important implications for synthetic biology approaches aiming to produce high-value specialized metabolites in heterologous hosts.
authors.affiliation: Plant Biochemistry Laboratory, Center for Synthetic Biology, VILLUM Research Center "Plant Plasticity," Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen Denmark.
authors.forename: Jean-Etienne
authors.initials: JE
authors.lastname: Bassard
authors.affiliation: Plant Biochemistry Laboratory, Center for Synthetic Biology, VILLUM Research Center "Plant Plasticity," Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen Denmark.|Carlsberg Research Laboratory, Gamle Carlsberg Vej 10, DK-1799 Copenhagen V, Denmark.
authors.forename: Birger Lindberg
authors.initials: BL
authors.lastname: Møller
authors.affiliation: Plant Biochemistry Laboratory, Center for Synthetic Biology, VILLUM Research Center "Plant Plasticity," Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen Denmark.|Feedstocks Division, Joint BioEnergy Institute, Emeryville, CA 94608 USA.
authors.forename: Tomas
authors.initials: T
authors.lastname: Laursen
keywords: Channeling; Cytochrome P450 oxidoreductase; Cytochromes P450; Membrane environment; Metabolon; Solvent environment