title: Comparison of the Novel Oxa-48 and Kpc K-SeT Assay, and Blue-Carba Test for the Detection of Carbapenemase-Producing Enterobacteriaceae Using PCR as a Reference Method.
abstract: BACKGROUND
  Rapid, simple, and accurate laboratory detection of carbapenemases is very important for proper antibiotic therapy and infection control.
  
  
  METHODS
  In this study, carbapenem-nonsusceptible Enterobacteriaceae (CRE) isolates were used to evaluate the performance of a new lateral flow immunochromatographic (IC) assay, the OXA-48 and KPC K-SeT assay, and modified Blue-Carba test (BCT) for the rapid detection of OXA-48 carbapenemase in comparison with polymerase chain reaction (PCR) amplification. These CREs of various enterobacterial species were isolated from various clinical samples including OXA-48 (47), NDM-1 (6), KPC-1 (1), IMP-1 (1), VIM-2,-4 (2), IMP-2 (1), OXA-51 (1), and OXA-23 (1) producers.
  
  
  RESULTS
  The OXA-48 K-SeT test detected all OXA-48 carbapenemase producers with 100% sensitivity and specificity. The BCT detected carbapenemase producers with 93% sensitivity and 100% specificity.
  
  
  CONCLUSIONS
  Both IC assays and BCT tests have good performance and are easy to perform, rapid, simple to interpret, and highly sensitive. We suggest that BCT can be used initially as an accurate, inexpensive, and rapid phenotypic confirmation test to identify Class A, B, and D carbapenemases in the routine diagnostic microbiology laboratory, thus allowing the detection of carbapenemase activity directly from bacterial cultures.
authors.forename: Fatma
authors.initials: F
authors.lastname: Erdem
authors.forename: Ayham
authors.initials: A
authors.lastname: Abulaila
authors.forename: Zerrin
authors.initials: Z
authors.lastname: Aktas
authors.forename: Oral
authors.initials: O
authors.lastname: Oncul
mesh_terms: D001426:Bacterial Proteins; D015780:Carbapenems; D002846:Chromatography, Affinity; D004755:Enterobacteriaceae; D006801:Humans; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases