title: Matrix stiffness regulates the proliferation, stemness and chemoresistance of laryngeal squamous cancer cells.
abstract: Increasing evidence shows that matrix stiffness plays a critical role in affecting the phenotype and behavior of tumor cells. We report that matrix stiffness significantly regulated the proliferation and chemotherapeutic response of Hep-2 cells. Increasing substrate stiffness promotes the proliferation of Hep-2 cells. When cultured on soft gels, Hep-2 cells expressed higher level of stem cell markers. Following treatment with cisplatin or 5-FU, we observed reduced apoptosis in Hep-2 cells cultured on soft supports. Sox2 is essential for the chemoresistance of Hep-2 cells cultured in soft stiffness. Our results demonstrated that Sox2 promotes chemoresistance of Hep-2 cells in soft stiffness via upregulating the expression of ABCG2. Our findings will provide a new platform for the future design of anticancer drugs based on the biophysical properties of the tumor site.
authors.affiliation: Department of Otolaryngology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Lian
authors.initials: L
authors.lastname: Hui
authors.affiliation: Department of Otolaryngology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Jingru
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Otolaryngology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Otolaryngology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000378:metabolism*; D000180:Acrylic Resins / Q000737:chemistry; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000503:physiopathology*; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000494:pharmacology; D006258:Head and Neck Neoplasms / Q000503:physiopathology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000503:physiopathology*; D009363:Neoplasm Proteins / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D000180:Acrylic Resins; D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; C016680:polyacrylamide gels; D003094:Collagen; D002945:Cisplatin; D005472:Fluorouracil