title: Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules.
abstract: The discovery of antibodies that bind to targets with high affinity is now a routine exercise. However, it is still challenging to screen for candidates that, in addition to having excellent biological properties, also have optimal biophysical characteristics. Here, we describe a simple HPLC-based screening method to assess for developability factors earlier in the discovery process.
authors.affiliation: Merrimack Pharmaceuticals, Inc., Suite B7201, 1 Kendall Square, Cambridge, MA, 02139, USA. nkohli@merrimack.com.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kohli
authors.affiliation: Merrimack Pharmaceuticals, Inc., Suite B7201, 1 Kendall Square, Cambridge, MA, 02139, USA.
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Geddie
mesh_terms: D000327:Adsorption; D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D055550:Protein Stability
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal
keywords: Colloidal stability; Developability; Size exclusion chromatography; Standup monolayer adsorption chromatography; Zenix column