title: Glycan-Functionalized Microgels for Scavenging and Specific Binding of Lectins.
abstract: Lectins are proteins with a well-defined carbohydrate recognition domain. Many microbial proteins such as bacterial toxins possess lectin or lectin-like binding domains to interact with cell membranes that are decorated with glycan recognition motifs. We report a straightforward way to prepare monodisperse and biocompatible polyethylene glycol microgels, which carry glycan motifs for specific binding to lectins. The sugar-functionalized colloids exhibit a wide mesh size and a highly accessible volume. The microgels are prepared via drop-based microfluidics combined with radical polymerization. GSII and ECL are used as model lectins that bind specifically to the corresponding carbohydrates, namely, GlcNAc and LacNAc. LacNAc microgels bind ECL with a high capacity and high affinity (Kd≈ 0.5 to 1 μM), suggesting multivalent binding of the lectin to the LacNAc-decorated flexible microgel network. Glycan-functionalized microgels present a useful tool for lectin scavenging in biomedical applications.
authors.affiliation: DWI - Leibniz Institute for Interactive Materials, RWTH Aachen University , Forckenbeckstraße 50, 52076 Aachen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Jans
authors.affiliation: Laboratory for Biomaterials Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University , Pauwelsstr. 20, 52074 Aachen, Germany.
authors.forename: Ruben R
authors.initials: RR
authors.lastname: Rosencrantz
authors.affiliation: Department of Internal Medicine III, University Hospital, RWTH Aachen University , Pauwelsstr. 30, 52074 Aachen, Germany.
authors.forename: Ana D
authors.initials: AD
authors.lastname: Mandić
authors.affiliation: DWI - Leibniz Institute for Interactive Materials, RWTH Aachen University , Forckenbeckstraße 50, 52076 Aachen, Germany.
authors.forename: Naveed
authors.initials: N
authors.lastname: Anwar
authors.affiliation: Department of Internal Medicine III, University Hospital, RWTH Aachen University , Pauwelsstr. 30, 52074 Aachen, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Boesveld
authors.affiliation: Department of Internal Medicine III, University Hospital, RWTH Aachen University , Pauwelsstr. 30, 52074 Aachen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Trautwein
authors.affiliation: DWI - Leibniz Institute for Interactive Materials, RWTH Aachen University , Forckenbeckstraße 50, 52076 Aachen, Germany.
authors.forename: Martin
authors.identifier: http://orcid.org/0000-0002-5955-4185
authors.initials: M
authors.lastname: Moeller
authors.affiliation: Department of Internal Medicine III, University Hospital, RWTH Aachen University , Pauwelsstr. 30, 52074 Aachen, Germany.
authors.forename: Gernot
authors.initials: G
authors.lastname: Sellge
authors.affiliation: Laboratory for Biomaterials Institute for Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University , Pauwelsstr. 20, 52074 Aachen, Germany.
authors.forename: Lothar
authors.identifier: http://orcid.org/0000-0002-3654-0397
authors.initials: L
authors.lastname: Elling
authors.affiliation: DWI - Leibniz Institute for Interactive Materials, RWTH Aachen University , Forckenbeckstraße 50, 52076 Aachen, Germany.
authors.forename: Alexander J C
authors.identifier: http://orcid.org/0000-0003-0142-8001
authors.initials: AJC
authors.lastname: Kuehne
mesh_terms: D005782:Gels / Q000138:chemical synthesis / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D044085:Microfluidics / Q000379:methods; D058105:Polymerization; D011134:Polysaccharides / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D005782:Gels; D037102:Lectins; D011134:Polysaccharides