title: Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model.
abstract: This study was conducted to investigate impacts of dietary protein levels on gut bacterial community and gut barrier. The intestinal microbiota of finishing pigs, fed with 16%, 13% and 10% crude protein (CP) in diets, respectively, were investigated using Illumina MiSeq sequencing. The ileal bacterial richness tended to decrease when the dietary protein concentration reduced from 16% to 10%. The proportion of Clostridium_sensu_stricto_1 in ileum significantly decreased, whereas Escherichia-Shigella increased with reduction of protein concentration. In colon, the proportion of Clostridium_sensu_stricto_1 and Turicibacter increased, while the proportion of RC9_gut_group significantly decreased with the dietary protein reduction. Notably, the proportion of Peptostreptococcaceae was higher in both ileum and colon of 13% CP group. As for metabolites, the intestinal concentrations of SCFAs and biogenic amines decreased with the dietary protein reduction. The 10% CP dietary treatment damaged ileal mucosal morphology, and decreased the expression of biomarks of intestinal cells (Lgr5 and Bmi1), whereas the expression of tight junction proteins (occludin and claudin) in 13% CP group were higher than the other two groups. In conclusion, moderate dietary protein restriction (13% CP) could alter the bacterial community and metabolites, promote colonization of beneficial bacteria in both ileum and colon, and improve gut barrier function.
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Peixin
authors.initials: P
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Peixia
authors.initials: P
authors.lastname: Song
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Xiyue
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.|Department of Internal Medicine, Department of Biochemistry, Center for Autophagy Research, University of Texas Southwestern Medical Center, Dallas, TX 75390-9113, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Ma
mesh_terms: D000821:Animal Feed; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D062445:Claudin-1 / Q000235:genetics / Q000378:metabolism; D003013:Clostridium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D018753:Diet, Protein-Restricted / Q000379:methods*; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism; D004063:Digestion / Q000187:drug effects* / Q000502:physiology; D004925:Escherichia / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism; D000068536:Bacillota / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D000069196:Gastrointestinal Microbiome / Q000187:drug effects* / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D014644:Genetic Variation; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D010462:Peptostreptococcus / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D063150:Polycomb Repressive Complex 1 / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D012760:Shigella / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D013552:Swine; D062725:Tight Junction Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001679:Biogenic Amines; D062445:Claudin-1; D004044:Dietary Proteins; D005232:Fatty Acids, Volatile; D062793:Occludin; D043562:Receptors, G-Protein-Coupled; D062725:Tight Junction Proteins; D063150:Polycomb Repressive Complex 1