title: Characterization and genetic mapping of the β-diketone deficient eceriferum-b barley mutant.
abstract: The barley eceriferum-b.2 (cer-b.2) mutant produces glossy leaf sheaths and is deficient in the cuticular wax component 14,16-hentriacontanedione. The mutated gene maps to a 1.3-cM interval on chromosome 3HL flanked by the genes MLOC_10972 and MLOC_69561. The cuticular wax coating of leaves and stems in many grass species is responsible for the plants' glaucous appearance. A major component of the wax is a group of β-diketone compounds. The barley eceriferum-b.2 (cer-b.2) mutant produces glossy leaf sheaths and is deficient for the compound 14,16-hentriacontanedione. A linkage analysis based on 708 gametes allowed the gene responsible for the mutant phenotype to be mapped to a 1.3-cM interval on chromosome 3HL flanked by the two genes MLOC_10972 and _69561. The product of the wild type allele may represent a step in the β-diketone synthesis pathway.
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai, 201602, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Mishina
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Jiecai
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Eco-Environmental Engineering, Qinghai University, Xining, 810016, Qinghai, China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Duan
authors.affiliation: Department of Biology, South University of Science and Technology of China, Shenzhen, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Ma
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Aidong
authors.initials: A
authors.lastname: Wang
authors.affiliation: Lanzhou Center for Oil and Gas Resources, Institute of Geology and Geophysics, Chinese Academy of Sciences, Donggang west Road 382, Lanzhou, 730000, China.
authors.forename: Qianxiang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Komatsuda
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China. guoxiong@lzb.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, China. guoxiong@lzb.ac.cn.
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Chen
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D008040:Genetic Linkage; D001467:Hordeum / Q000737:chemistry / Q000235:genetics*; D007659:Ketones / Q000737:chemistry*; D009154:Mutation; D010641:Phenotype; D019441:Plant Epidermis / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D014885:Waxes / Q000737:chemistry*
chemical_list: D007659:Ketones; D014885:Waxes