title: Influence of Al3+on the titer of spiramycin and effective components in fermentor.
abstract: Spiramycin is a multicomponent antibiotic, and different components have different antibacterial activities. In Streptomyces spiramyceticus 16-10-2, spiramycin II and spiramycin III (SPMII and SPMIII) are the main components, while spiramycin I (SPMI) needs to be controlled below 12%. Based on this, the influences of Al3+on total spiramycin titer and components were investigated in this work. Those experiments were mainly performed in 15 L fermentor and Al3+made a great improvement in spiramycin titer. The optimal adding concentration and adding time of Al3+were 0.32 g/L at 12 hr. Under this condition, spiramycin titer was increased by 19.51% compared with the control. Moreover, the percentage of SPMII and SPMIII was increased by 7.14%. At the same time, the time of mycelia autolysis was lengthened. In addition, the specific activities of acetyl-CoA synthetase, acetate kinase, acetylphosphotransferase, and acylating enzyme were much higher than those of control. The content of acetic acid and succinic acid was beyond 3 and 4.5 times than that of control, respectively.
authors.affiliation: a The State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: a The State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , China.
authors.forename: Fengxian
authors.initials: F
authors.lastname: Hu
authors.affiliation: a The State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Ye
authors.affiliation: b Research and Development Department , Topfond Pharmaceutical Co., Ltd , Zhumadian , Henan , China.
authors.forename: Heping
authors.initials: H
authors.lastname: Lv
authors.affiliation: a The State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
mesh_terms: D000215:Acylation; D000535:Aluminum / Q000378:metabolism*; D000617:Aminoglycosides / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D019149:Bioreactors; D002412:Cations / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology; D015572:Spiramycin / Q000031:analogs & derivatives / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002412:Cations; C017186:demycarosylturimycin H; C033163:acetylspiramycin; C087816:spiramycin III; D015572:Spiramycin; D000535:Aluminum
keywords: Al3+; Spiramycin; Streptomyces spiramyceticus 16-10-2