title: [MiR-26b inhibits proliferation, invasion, and migration of glioma by targeting cyclooxygenase-2].
abstract: To explore the expressions of miR-26b and cyclooxygenase (COX)-2 in different grades of gliomas and the effect of miR-26b on glioma cell proliferation, invasion and migration.  Methods: Western blot and Real-time quantitative PCR (qRT-PCR) were used to detect the expression levels of miR-26b and COX-2 in different grades of gliomas. Human glioma cells were transfected with miR-26b mimics. qRT-PCR was employed to detect the mRNA expressions of miR-26b and COX-2 after miR-26b mimics transfection, while dual-luciferase reporter assay was used to investigate the regulatory effect of miR-26b on COX-2. Cell counting kit-8 (CCK8), trans-well invasion assay and scratch assay were used to detect the proliferation, invasion and migration of human U87 glioma cells after miR-26b mimic transfection, respectively. The antitumor effect of miR-26b was verified by evaluating the volume and weight of tumor in nude mice.  Results: With the increase in tumor grades, the expression of miR-26b was significantly decreased (P<0.05), while COX-2 expression was increased (P<0.001). Dual luciferase assay confirmed that miR-26b could directly regulate the protein expression of COX-2. MiR-26b mimics could significantly reduce the expression of COX-2 (P<0.05) and suppress the proliferation, invasion and migration of glioma cell (P<0.05). The volume and weight of tumor in MiR-26b mimics transfection group were smaller than those in the control group.  Conclusion: Overexpression of miR-26b may inhibit proliferation, invasion, and migration of glioma by suppressing the expression of COX-2. Therefore, the miR-26b/COX-2 pathway might be a therapeutic target in glioma.
authors.affiliation: Department of Neurosurgery, First Affi liated Hospital of Hainan Medical College, Haikou 570102; Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
authors.forename: Zizhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
authors.forename: Chuanyi
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
authors.forename: Wangqing
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D051546:Cyclooxygenase 2 / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology*; D005910:Glioma / Q000737:chemistry* / Q000503:physiopathology*; D006801:Humans; D008819:Mice, Nude; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D051546:Cyclooxygenase 2; D035683:MicroRNAs; C528163:MIRN26 microRNA, human