title: Immunomodulatory effect of protease hydrolysates from ovotransferrin.
abstract: Proteins and bioactive peptides in avian egg white exert diverse biological activities. This study is designed to investigate the effect of protease hydrolysates from ovotransferrin (OVT) on bone marrow-derived dendritic cells (BMDCs) maturation. The results show that OVT-derived pepsin hydrolysate effectively inhibits lipopolysaccharide (LPS)-induced BMDCs maturation by reducing the expression levels of MHC-II, CD83, CD86 and the production of TNF-α, IL-12p70, and RANTES, but increases the production of IL-10. In addition, OVT-derived pepsin hydrolysate impairs the ability of LPS-stimulated BMDCs to induce allogeneic T lymphocyte proliferation and decreases the production of IFN-γ by activated T cells. In contrast, OVT-derived trypsin hydrolysate induces DCs maturation in terms of increasing the expression levels of MHC-II and the costimulatory molecules CD83 and CD86 and the production of TNF-α, IL-12p70 and RANTES. Furthermore, OVT-derived trypsin hydrolysate improves the ability of LPS-stimulated DCs to induce allogeneic T lymphocyte activation. Blockage of LPS-induced p38 MAPK and JNK activation and inducing ERK activation contribute to the inhibitory effect of OVT-derived pepsin hydrolysate on DCs, whereas OVT-derived trypsin hydrolysate induces DCs maturation through JNK and ERK activation. These results indicate that OVT-derived protease hydrolysate have an immunomodulatory function and could be applied as a potential functional food ingredient to regulate body immunity by modulating DC maturation.
authors.affiliation: Key Laboratory of Natural Product and Functional Food of Jiangxi, Nanchang 330045, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Xu
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Tu
authors.forename: Huaying
authors.initials: H
authors.lastname: Du
authors.forename: Yulan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Gaoxiang
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D003207:Conalbumin / Q000737:chemistry* / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006868:Hydrolysis; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology; D014357:Trypsin / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D003207:Conalbumin; D007136:Immunoglobulins; D007155:Immunologic Factors; D016753:Interleukin-10; D018664:Interleukin-12; D008562:Membrane Glycoproteins; D011492:Protein Hydrolysates; D014357:Trypsin; D014409:Tumor Necrosis Factor-alpha; D000097802:CD83 Antigen