title: Microfilament Depolymerization Is a Pre-requisite for Stem Cell Formation DuringIn vitroShoot Regeneration inArabidopsis.
abstract: De novoshoot regeneration is widely used in fundamental studies and agricultural applications. Actin microfilaments are involved in many aspects of plant cell division, cell morphogenesis and cell signal transduction. However, the function of actin microfilaments duringde novoshoot regeneration is poorly understood. Here, we investigated the organization of actin microfilaments during this process and found that stem cell formation was associated with microfilament depolymerization. Furthermore, inhibition of microfilament depolymerization by phalloidin treatment or downregulation of actin depolymerizing factors (ADFs) restrained stem cell initiation and shoot regeneration. Inhibition ofADFexpression affected the architecture of microfilaments during stem cell formation, and the polar transport and distribution of auxin were also disrupted. Together, our results demonstrate that organization of the microfilament cytoskeleton play important roles in stem cell formation and shoot meristem induction during shoot regeneration.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University Taian, China.
authors.forename: Li Ping
authors.initials: LP
authors.lastname: Tang
authors.affiliation: Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University Taian, China.
authors.forename: Xiao Ming
authors.initials: XM
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University Taian, China.
authors.forename: Yu Xiu
authors.initials: YX
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University Taian, China.
authors.forename: Xian Sheng
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University Taian, China.
authors.forename: Ying Hua
authors.initials: YH
authors.lastname: Su
keywords: Arabidopsis; auxin distribution; auxin polar transport; microfilament depolymerization; shoot regeneration; stem cell formation