title: Sex chromosome complement involvement in angiotensin receptor sexual dimorphism.
abstract: This study aimed to define whether sex chromosome complement (SCC) may differentially modulate sex differences in relative gene expression of basal Agtr1a, Agtr2, and Mas1 receptors at fore/hindbrain nuclei and at medulla/cortical kidney. Samples were collected from gonadectomized male (XX and XY) and female (XX and XY) mice of the "four core genotypes" model. At brain level, a SCC effect at the area postrema was demonstrated. An increase in mRNA level of Agtr1a and Agtr1a/Agtr2 ratio in XY-SCC mice was associated with a decrease in Mas1 compared to XX-SCC mice. In the renal cortex, a SCC effect for Agtr2 and Mas1 was observed. Regardless of sex (male or female), XX-SCC mice expressed higher levels of mRNA Agtr2 and Mas1 than XY-SCC mice {F(1,12) = 6,126,p < 0.05; F(1,21) = 5,143,p < 0.05}. Furthermore, XX-female mice showed a significant increase in Mas1 expression compared to XY-female mice. These results reveal a SCC modulatory effect at central and kidney level on angiotensin receptor expression, with an enhancement of the vasodilatory arm in XX-mice and an increase in the vasoconstriction arm in XY-mice, which may underlie sex differences in the regulation of arterial pressure.
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Florencia M
authors.initials: FM
authors.lastname: Dadam
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Carla D
authors.initials: CD
authors.lastname: Cisternas
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Ana F
authors.initials: AF
authors.lastname: Macchione
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Andrea
authors.initials: A
authors.lastname: Godino
authors.affiliation: Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, FMRP, USP, Brazil.
authors.forename: José
authors.initials: J
authors.lastname: Antunes-Rodrigues
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: María J
authors.initials: MJ
authors.lastname: Cambiasso
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Vivas
authors.affiliation: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, Argentina. Electronic address: xcaeiro@immf.uncor.edu.
authors.forename: Ximena E
authors.initials: XE
authors.lastname: Caeiro
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics / Q000378:metabolism*; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012727:Sex Characteristics; D012730:Sex Chromosomes / Q000378:metabolism*
chemical_list: C000715471:Mas1 protein, mouse; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D043562:Receptors, G-Protein-Coupled
keywords: Angiotensin receptors; Area postrema; Four core genotype mouse model; Gene expression; Renal cortex; Renin angiotensin system