title: Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in Brassica napus L.
abstract: Different mitotype-specific markers were developed to distinguish different cytoplasms in Brassica napus L. Mitotype-specific markers have been developed to distinguish different mitotypes in plant. And use of molecular markers to identify different mitotypes in Brassica napus would enhance breeding efficiency. Here, we comparatively analyzed six sequenced mitochondrial genomes in Brassica napus and identified collinear block sequences and mitotype-specific sequences (MSSs) of these mitochondrial genomes. The collinear block sequences between mitochondrial genomes of nap, cam, and pol cytoplasmic male sterility (CMS) lines were higher than those of other lines. After comparative analysis of the six sequenced mitochondrial genomes (cam, nap, ole, pol CMS, ogu CMS, and hau CMS), 90 MSSs with sizes ranging from 101 to 9981 bp and a total length of 103,756 bp (accounting for 6.77% of the mitochondrial genome sequences) were identified. Additionally, 12 mitotype-specific markers were developed based on the mitochondrial genome-specific sequences in order to distinguish among these different mitotypes. Cytoplasms of 570 different inbred lines collected across scientific research institutes in China were identified using the MSS markers developed in our study. In addition to confirming the accuracy of the cytoplasmic identification, we also identified mitotypes that have not been reported in Brassica napus. Our study may provide guidance for the classification of different mitotypes in B. napus breeding.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.|College of Life Science, Xinyang Normal University, Xinyang, 464000, People's Republic of China.
authors.forename: Shuangping
authors.initials: S
authors.lastname: Heng
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Fengyi
authors.initials: F
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wei
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Kaining
authors.initials: K
authors.lastname: Hu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yi
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Chaozhi
authors.initials: C
authors.lastname: Ma
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Tu
authors.affiliation: Centre for Plant Genetics and Breeding, School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
authors.forename: Ping
authors.initials: P
authors.lastname: Si
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Tingdong
authors.initials: T
authors.lastname: Fu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. jxshen@mail.hzau.edu.cn.
authors.forename: Jinxiong
authors.identifier: http://orcid.org/0000-0002-1225-7407
authors.initials: J
authors.lastname: Shen
mesh_terms: D029688:Brassica napus / Q000378:metabolism* / Q000502:physiology; D001947:Breeding; D003593:Cytoplasm / Q000378:metabolism* / Q000502:physiology; D018745:Genome, Plant / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins
keywords: Brassica; Brassica napus; MAS (marker-assisted selection); Mitochondria; Mitotype-specific sequence