title: Long-term liquid storage and reproductive evaluation of an innovative boar semen extender (Formula12®) containing a non-reducing disaccharide and an enzymatic agent.
abstract: There are no reports of saccharolytic enzymes being used in the preparation of formulations for animal semen extenders. In the present study, the use of an innovative semen extender (Formula12®) in the long-term liquid storage of boar semen at 17°C was evaluated. The formulation included use of a disaccharide (sucrose) as the energy source precursor coupled to an enzymatic agent (invertase). The innovative extender was evaluated and compared in vitro to a commercial extender (Vitasem LD®) for the following variables: Total Motility (TM), Forward Progressive Motility (FPM), sperm morphology, membrane integrity, acrosome integrity, and chromatin instability. Boar sperm diluted in Formula12®and stored for 12 days at 17°C maintained a commercially acceptable FPM (>70%). Using the results from the in vitro study, an AI field trial was performed. A total of 170 females were inseminated (135 with Formula12®and 35 with Vitasem LD®). The pregnancy rates were 97.8% compared with 91.4%, and the farrowing rates were 96.3% compared with 88.6% when Formula12®and Vitasem LD®were used, respectively. The mean number of piglets born/sow were 14.92±0.46 compared with 13.83±0.70, and the number of piglets born alive/sow were 14.07±0.46 compared with 12.12±0.70 (P<0.05). The results obtained in this study demonstrated that use of the innovative concept to provide a precursor of glucose and fructose as energy sources for an enzymatic agent in an extender allowed for meeting the metabolic requirements of boar sperm during storage at 17°C. It is suggested that there was a beneficial effect on fertilizing capacity of boar sperm in the female reproductive tract with use of these technologies.
authors.affiliation: Department of Veterinary Science, University of Parma, Via del Taglio, 10, 43126 Parma, Italy. Electronic address: carla.bresciani@unipr.it.
authors.forename: Carla
authors.initials: C
authors.lastname: Bresciani
authors.affiliation: Department of Pharmacy, University of Parma, Parco Area delle Scienze, 27/A, 43124 Parma, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Bianchera
authors.affiliation: Department of Pharmacy, University of Parma, Parco Area delle Scienze, 27/A, 43124 Parma, Italy.
authors.forename: Ruggero
authors.initials: R
authors.lastname: Bettini
authors.affiliation: Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Buschini
authors.affiliation: Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Marchi
authors.affiliation: Department of Veterinary Science, University of Parma, Via del Taglio, 10, 43126 Parma, Italy.
authors.forename: Clotilde Silvia
authors.initials: CS
authors.lastname: Cabassi
authors.affiliation: Department of Veterinary Science, University of Parma, Via del Taglio, 10, 43126 Parma, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Sabbioni
authors.affiliation: Department of Veterinary Science, University of Parma, Via del Taglio, 10, 43126 Parma, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Righi
authors.affiliation: Suivet(®) 42123, Reggio nell'Emilia, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Mazzoni
authors.affiliation: Department of Veterinary Science, University of Parma, Via del Taglio, 10, 43126 Parma, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Parmigiani
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000187:drug effects / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013048:Specimen Handling; D013081:Sperm Motility; D013094:Spermatozoa; D013552:Swine / Q000502:physiology*; D013997:Time Factors
keywords: Boar semen extender; Invertase; Long-term storage; Reproductive efficiency; Sucrose