title: Synchrotron microtomography to evaluate effects of different polychemotherapy drugs on cortical bone structure.
abstract: To determine the effects of different polychemotherapy drugs on cortical bone structure, the femur diaphysis of rats were treated with two different chemotherapy drugs, AC (doxorubicin + cyclophosphamide) and TC (docetaxel + cyclophosphamide), and evaluated by 3D morphological analysis using synchrotron radiation microtomography.
  
  
  
  Wistar rats were classified into three groups. One group received doses of docetaxel and cyclophosphamide (G1) - TC regimen; a second group received doses of doxorubicin and cyclophosphamide (G2) - AC regimen; while a control group (G0) received no further treatment. 3D tomographic images of the rats' femurs were obtained at the SYRMEP (Synchrotron Radiation for Medical Physics) beamline at the ELETTRA Synchrotron Laboratory in Trieste, Italy, using monochromatic X-rays with resolution of 9 μm.
  
  
  
  It could be shown that the treatment caused significant differences in morphological parameters measured from the 3D images of femur diaphysis of rats, among the studied groups, complementing a previous study using stereological methods, biochemistry and electron microscopy.
  
  
  
  Our results indicate that the same process of osteoporosis caused by advancing age might occur in young women treated with docetaxel + cyclophosphamide (TC) and doxorubicin + cyclophosphamide (AC). 3D microtomography was shown to be an outstanding technique for bone analysis.
authors.affiliation: a Physics Institute, State University of Rio de Janeiro (UERJ) , Rio de Janeiro , Brazil.
authors.forename: Liebert P
authors.initials: LP
authors.lastname: Nogueira
authors.affiliation: b Nuclear Engineering Program , COPPE/Federal University of Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Rita
authors.initials: R
authors.lastname: Alessio
authors.affiliation: a Physics Institute, State University of Rio de Janeiro (UERJ) , Rio de Janeiro , Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mantuano
authors.affiliation: b Nuclear Engineering Program , COPPE/Federal University of Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Sena
authors.affiliation: c Radiological Sciences Department , State University of Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: de Almeida
authors.affiliation: d Sincrotrone Trieste SCpA , Trieste , Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Tromba
authors.affiliation: e UEZO , State University of West Zone , Rio de Janeiro , Brazil.
authors.forename: Andre P
authors.initials: AP
authors.lastname: Almeida
authors.affiliation: f CNEN , National Nuclear Energy Commission , Rio de Janeiro , Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Salata
authors.affiliation: a Physics Institute, State University of Rio de Janeiro (UERJ) , Rio de Janeiro , Brazil.
authors.forename: Marcos V G
authors.initials: MVG
authors.lastname: Colaço
authors.affiliation: b Nuclear Engineering Program , COPPE/Federal University of Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Delson
authors.initials: D
authors.lastname: Braz
authors.affiliation: a Physics Institute, State University of Rio de Janeiro (UERJ) , Rio de Janeiro , Brazil.
authors.forename: Regina C
authors.initials: RC
authors.lastname: Barroso
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage*; D003520:Cyclophosphamide / Q000008:administration & dosage; D018483:Diaphyses / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D000077143:Docetaxel; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D017356:Synchrotrons; D043823:Taxoids / Q000008:administration & dosage; D055114:X-Ray Microtomography
chemical_list: D043823:Taxoids; D000077143:Docetaxel; D004317:Doxorubicin; D003520:Cyclophosphamide
keywords: Microtomography; chemotherapy drugs; cortical bone porosity; morphological analysis; synchrotron radiation