title: The role of oxidative stress in α-amanitin-induced hepatotoxicityin an experimental mouse model.
abstract: This study aimed to evaluate oxidative stress markers of liver tissue in a mouse α-amanitin poisoning model with three different toxin levels.
  
  
  
  The mice were randomly divided into Group 1 (control), Group 2 (0.2 mg/kg), Group 3 (0.6 mg/kg), and Group 4 (1.0 mg/kg). The toxin was injected intraperitoneally and 48 h of follow-up was performed before sacrifice.
  
  
  
  Median superoxide dismutase activities of liver tissue in Groups 3 and 4 were significantly higher than in Group 1 (for both, P = 0.001). The catalase activity in Group 2 was significantly higher, but in Groups 3 and 4 it was significantly lower than in Group 1 (for all, P = 0.001). The glutathione peroxidase activities in Groups 2, 3, and 4 were significantly higher than in Group 1 (P = 0.006, P = 0.001, and P = 0.001, respectively). The malondialdehyde levels of Groups 3 and 4 were significantly higher than Group 1 (P = 0.015 and P = 0.003, respectively). The catalase activity had significant correlations with total antioxidant status and total oxidant status levels (r = 0.935 and r = -0.789, respectively; for both, P < 0.001).
  
  
  
  Our findings support a significant role for increased oxidative stress in α-amanitin-induced hepatotoxicity.
authors.affiliation: Department of Emergency Medicine, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors.forename: Zerrin Defne
authors.initials: ZD
authors.lastname: Dündar
authors.affiliation: Department of Emergency Medicine, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ergin
authors.affiliation: Department of Biochemistry, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Kilinç
authors.affiliation: Department of Emergency Medicine, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors.forename: Tamer
authors.initials: T
authors.lastname: Çolak
authors.affiliation: Department of Medical Pathology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors.forename: Pembe
authors.initials: P
authors.lastname: Oltulu
authors.affiliation: Department of Emergency Medicine, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
authors.forename: Başar
authors.initials: B
authors.lastname: Cander
mesh_terms: D053959:Alpha-Amanitin / Q000633:toxicity*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D053959:Alpha-Amanitin; D008315:Malondialdehyde; D005979:Glutathione Peroxidase