title: Lactobacillusspecies mediated synthesis of silver nanoparticles and their antibacterial activity against opportunistic pathogensin vitro.
abstract: Introduction:Application of lactic acid bacteria for synthesis of silver (AG) nanoparticles (NPs) could be a good ecological friendly alternative to chemical and physical methods. The objective of this study was to investigate the biosynthesis of silver NPs usingLactobacillusstrains and to compare their monosaccharide composition of capsular exopolysaccharides and the antibacterial activity of synthesized nanoparticles.Methods:The washed cells of 22Lactobacillusstrains were used forin vitrosilver nanoparticle biosynthesis from silver nitrate solution. The NPs formation was confirmed by UV-visible spectroscopy and transmission electron microscopy (TEM) analysis. TEM micrographs were used for the evaluation of NPs size. The monosaccharide composition of capsular exopolysaccharides was determined using GC/MS analysis. The antimicrobial activity was determined by agar well diffusion assay.Results:The capsular layers ofLactobacillusstrains contained heteropolysaccharides that were composed mostly of glucose, mannose, galactose and rhamnose in a different molar ratio. It was found that Ag NPs with large size (30.65 ± 5.81 nm) obtained fromL. acidophilus58p were more active againstS. epidermidis, E. coli, K. pneumonia,S. flexneriandS. sonneicompared with Ag NPs fromL. plantarum92T (19.92 ± 3.4 nm).Conclusion:The size and antibacterial activities of Ag NPs were strain-dependent and such characteristics may be due to the capsular biopolymer composition ofLactobacillusstrains used for Ag NPs synthesis.
authors.affiliation: Department of Physiology of Industrial Microorganisms, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine.
authors.forename: Inna
authors.initials: I
authors.lastname: Garmasheva
authors.affiliation: Department of Physiology of Industrial Microorganisms, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Kovalenko
authors.affiliation: Department of Physiology of Industrial Microorganisms, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine.
authors.forename: Sergey
authors.initials: S
authors.lastname: Voychuk
authors.affiliation: Laboratory of Biological Polymer Compounds, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine.
authors.forename: Andriy
authors.initials: A
authors.lastname: Ostapchuk
authors.affiliation: Department of Physiology of Industrial Microorganisms, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine.
authors.forename: Olena
authors.initials: O
authors.lastname: Livins'ka
authors.affiliation: Department of Physiology of Industrial Microorganisms, Zabolotny Institute of Microbiology and Virology, National Academy of Science of Ukraine, 03143 Kiev, Ukraine.
authors.forename: Ljubov
authors.initials: L
authors.lastname: Oleschenko
keywords: Antibacterial activity; Lactic acid bacteria; Nanobiotechnology; Opportunistic pathogens; Silver nanoparticles