title: Tracing sialoglycans on cell membrane via surface-enhanced Raman scattering spectroscopy with a phenylboronic acid-based nanosensor in molecular recognition.
abstract: Sialoglycan expression is critical for assessing various diseases progression. Especially, its abnormal levels are commonly believed to be associated with tumor and metastatic cancer types. While, complicated structures, multiple types and dynamic distributions make it challenging for in situ investigating sialoglycans at the physiological status. Herein, we developed a 4-mercaptophenylboronic acid (MPBA)-based surface-enhanced Raman scattering (SERS) nanosensor to in situ study sialoglycan levels and dynamic expression processes of different cell types based on molecular recognition between phenylboronic acid and sialoglycans at physiological condition. This nanosensor is designed by the MPBA decorated silver nanoparticle (AgNP), which is unique and multifunctional because of its three-in-one role involving the Raman signal enhancer (AgNP), the sensing reporter of MPBA and the target receptor based on the recognition of phenylboronic acid and sialoglycans. When this nanosensor binds to sialoglycans, the molecular vibrational modes of MPBA will change, which can be traced by ultrasensitive SERS technique. The superiority of this study is that we built the relation between the spectral changes of MPBA (relative intensities) in molecular recognition with the sialoglycan dynamic expression of cells. We believe that our SERS strategy could be further extended to explore crucial physiological processes and significant biological system that glycans are involved in.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Liang
authors.affiliation: Institute of Frontier Medical Science, Jilin University, Changchun 130021, People's Republic of China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Biying
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, People's Republic of China. Electronic address: xusp@jlu.edu.cn.
authors.forename: Shuping
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Frontier Medical Science, Jilin University, Changchun 130021, People's Republic of China. Electronic address: liang@jlu.edu.cn.
authors.forename: Chongyang
authors.initials: C
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: C510906:4-mercaptophenylboronic acid; D001897:Boronic Acids; D006017:Glycolipids; D013438:Sulfhydryl Compounds; C009255:sialoglycolipids
keywords: Dynamic expression; Nanosensor, In situ, SERS spectroscopy; Sialoglycans