title: Three-Dimensional Reconstruction of the Tissue-Specific Multielemental Distribution within Ceriodaphnia dubia via Multimodal Registration Using Laser Ablation ICP-Mass Spectrometry and X-ray Spectroscopic Techniques.
abstract: In this work, the three-dimensional elemental distribution profile within the freshwater crustacean Ceriodaphnia dubia was constructed at a spatial resolution down to 5 μm via a data fusion approach employing state-of-the-art laser ablation-inductively coupled plasma-time-of-flight mass spectrometry (LA-ICP-TOFMS) and laboratory-based absorption microcomputed tomography (μ-CT). C. dubia was exposed to elevated Cu, Ni, and Zn concentrations, chemically fixed, dehydrated, stained, and embedded, prior to μ-CT analysis. Subsequently, the sample was cut into 5 μm thin sections that were subjected to LA-ICP-TOFMS imaging. Multimodal image registration was performed to spatially align the 2D LA-ICP-TOFMS images relative to the corresponding slices of the 3D μ-CT reconstruction. Mass channels corresponding to the isotopes of a single element were merged to improve the signal-to-noise ratios within the elemental images. In order to aid the visual interpretation of the data, LA-ICP-TOFMS data were projected onto the μ-CT voxels representing tissue. Additionally, the image resolution and elemental sensitivity were compared to those obtained with synchrotron radiation based 3D confocal μ-X-ray fluorescence imaging upon a chemically fixed and air-dried C. dubia specimen.
authors.affiliation: Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
authors.forename: Stijn J M
authors.initials: SJ
authors.lastname: Van Malderen
authors.affiliation: Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
authors.forename: Brecht
authors.identifier: 0000-0002-6561-0948
authors.initials: B
authors.lastname: Laforce
authors.affiliation: Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Van Acker
authors.affiliation: Department of Applied Ecology and Environmental Biology, Ghent University , Jozef Plateaustraat 22, 9000 Ghent, Belgium.
authors.forename: Charlotte
authors.identifier: 0000-0001-9646-3744
authors.initials: C
authors.lastname: Nys
authors.affiliation: Department of Applied Ecology and Environmental Biology, Ghent University , Jozef Plateaustraat 22, 9000 Ghent, Belgium.
authors.forename: Maarten
authors.initials: M
authors.lastname: De Rijcke
authors.forename: Riet
authors.initials: R
authors.lastname: de Rycke
authors.forename: Michiel
authors.initials: M
authors.lastname: De Bruyne
authors.affiliation: Department of Physics and Astronomy, Ghent University , Proeftuinstraat 86, 9000 Ghent, Belgium.
authors.forename: Matthieu N
authors.initials: MN
authors.lastname: Boone
authors.affiliation: Department of Applied Ecology and Environmental Biology, Ghent University , Jozef Plateaustraat 22, 9000 Ghent, Belgium.
authors.forename: Karel
authors.initials: K
authors.lastname: De Schamphelaere
authors.affiliation: TOFWERK AG , Uttigenstrasse 22, 3600 Thun, Switzerland.
authors.forename: Olga
authors.initials: O
authors.lastname: Borovinskaya
authors.affiliation: Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
authors.forename: Björn
authors.initials: B
authors.lastname: De Samber
authors.affiliation: Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Vincze
authors.affiliation: Department of Analytical Chemistry, Ghent University , Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
authors.forename: Frank
authors.identifier: 0000-0002-1884-3853
authors.initials: F
authors.lastname: Vanhaecke
mesh_terms: D000818:Animals; D033361:Cladocera; D003300:Copper / Q000032:analysis; D021621:Imaging, Three-Dimensional; D053685:Laser Therapy; D013058:Mass Spectrometry; D064847:Multimodal Imaging; D009532:Nickel / Q000032:analysis; D014018:Tissue Distribution; D055114:X-Ray Microtomography; D015032:Zinc / Q000032:analysis
chemical_list: D003300:Copper; D009532:Nickel; D015032:Zinc