title: Continuous multi-modality brain imaging reveals modified neurovascular seizure response after intervention.
abstract: We developed a multi-modal brain imaging system to investigate the relationship between blood flow, blood oxygenation/volume, intracellular calcium and electrographic activity during acute seizure-like events (SLEs), both before and after pharmacological intervention. Rising blood volume was highly specific to SLE-onset whereas blood flow was more correlated with all eletrographic activity. Intracellular calcium spiked between SLEs and at SLE-onset with oscillation during SLEs. Modified neurovascular and ionic SLE responses were observed after intervention and the interval between SLEs became shorter and more inconsistent. Comparison of artery and vein pulsatile flow suggest proximal interference and greater vascular leakage prior to intervention.
authors.affiliation: The Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, M5S 3G9, Canada.
authors.forename: Dene
authors.initials: D
authors.lastname: Ringuette
authors.affiliation: Division of Fundamental Neurobiology, Toronto Western Research Institute, 60 Leonard Ave, Toronto, Ontario M5T 2R1, Canada.
authors.forename: Melanie A
authors.initials: MA
authors.lastname: Jeffrey
authors.affiliation: The Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, M5S 3G9, Canada; Division of Fundamental Neurobiology, Toronto Western Research Institute, 60 Leonard Ave, Toronto, Ontario M5T 2R1, Canada.
authors.forename: Suzie
authors.initials: S
authors.lastname: Dufour
authors.affiliation: The Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, M5S 3G9, Canada; Division of Fundamental Neurobiology, Toronto Western Research Institute, 60 Leonard Ave, Toronto, Ontario M5T 2R1, Canada.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Carlen
authors.affiliation: The Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, M5S 3G9, Canada; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Road, Toronto, Ontario, M5S 3G4, Canada.
authors.forename: Ofer
authors.initials: O
authors.lastname: Levi
keywords: (110.4234) Multispectral and hyperspectral imaging; (110.6150) Speckle imaging; (170.2520) Fluorescence microscopy