title: Development of competitive ELISA for the detection of bovine serum albumin using single-chain variable fragments.
abstract: Soluble anti-bovine serum albumin (BSA) single-chain variable fragments (scFvs) were expressed in E. Coli. HB2151. The antigen-binding equilibrium dissociation constant of the scFvs was determined to be 2.9 × 10-8M by surface plasmon resonance analysis. A competitive ELISA for the detection of BSA was developed using the antibody fragment above. The limits of detection (I10) and I50were 0.002 and 0.74 μg/ml respectively, with a recovery between 87.8 and 119.2% in spiked milk samples. The assay has the potential to be used to detect concentration of BSA in milk or other matrix instead of the ELISA based on traditional antibodies.
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China. Electronic address: zeranol@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Manman
authors.initials: M
authors.lastname: Lin
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Jieru
authors.initials: J
authors.lastname: Lin
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Hao
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Dan
authors.initials: D
authors.lastname: He
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Chongxin
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Cunzheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000276:immunology; D057127:Single-Chain Antibodies / Q000276:immunology*; D020349:Surface Plasmon Resonance
chemical_list: D000911:Antibodies, Monoclonal; D008894:Milk Proteins; D057127:Single-Chain Antibodies; D012710:Serum Albumin, Bovine
keywords: Bovine serum albumin; ELISA; Single-chain variable fragments