title: SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice.
abstract: Human LL-37 is a multifunctional antimicrobial peptide of cathelicidin family. It has been shown in recent studies that it can serve as a host's defense against influenza A virus. We now demonstrate in this study how signal peptide LL-37 (SP-LL-37) can be used in rice resistance against bacterial leaf blight and blast. We synthesized LL-37 peptide and subcloned in a recombinant pPZP vector with pGD1 as promoter. SP-LL-37 was introduced into rice plants by Agrobacterium mediated transformation. Stable expression of SP-LL-37 in transgenic rice plants was confirmed by RT-PCR and ELISA analyses. Subcellular localization of SP-LL-37-GFP fusion protein showed evidently in intercellular space. Our data on testing for resistance to bacterial leaf blight and blast revealed that the transgenic lines are highly resistant compared to its wildtype. Our results suggest that LL-37 can be further explored to improve wide-spectrum resistance to biotic stress in rice.
authors.affiliation: Department of Horticultural Life Science, Hankyong National University, Ansung, Korea.
authors.forename: In Hye
authors.initials: IH
authors.lastname: Lee
authors.affiliation: Department of Horticultural Life Science, Hankyong National University, Ansung, Korea.|Institute of Genetic Engineering, Hankyong National University, Ansung, Korea.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheongju, Korea.
authors.forename: Yong Gu
authors.initials: YG
authors.lastname: Cho
authors.affiliation: Department of Horticulture, Sunchon National University, Suncheon, Korea.
authors.forename: Ill Sup
authors.initials: IS
authors.lastname: Nou
authors.affiliation: Department of Horticultural Life Science, Hankyong National University, Ansung, Korea.|Institute of Genetic Engineering, Hankyong National University, Ansung, Korea.
authors.forename: Md Amdadul
authors.initials: MA
authors.lastname: Huq
authors.affiliation: Department of Crop Science, Chungbuk National University, Cheongju, Korea.
authors.forename: Franz Marielle
authors.initials: FM
authors.lastname: Nogoy
authors.affiliation: Department of Horticultural Life Science, Hankyong National University, Ansung, Korea.|Institute of Genetic Engineering, Hankyong National University, Ansung, Korea.
authors.forename: Kwon-Kyoo
authors.initials: KK
authors.lastname: Kang
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D060467:Disease Resistance / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism*; D010935:Plant Diseases / Q000517:prevention & control; D030821:Plants, Genetically Modified / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism; D054804:Cathelicidins
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D004247:DNA; D054804:Cathelicidins