title: Comparison of ultrastructural and nanomechanical signature of platelets from acute myocardial infarction and platelet activation.
abstract: Acute myocardial infarction (AMI) initiation and progression follow complex molecular and structural changes in the nanoarchitecture of platelets. However, it remains poorly understood how the transformation from health to AMI alters the ultrastructural and biomechanical properties of platelets within the platelet activation microenvironment. Here, we show using an atomic force microscope (AFM) that platelet samples, including living human platelets from the healthy and AMI patient, activated platelets from collagen-stimulated model, show distinct ultrastructural imaging and stiffness profiles. Correlative morphology obtained on AMI platelets and collagen-activated platelets display distinct pseudopodia structure and nanoclusters on membrane. In contrast to normal platelets, AMI platelets have a stiffer distribution resulting from complicated pathogenesis, with a prominent high-stiffness peak representative of platelet activation using AFM-based force spectroscopy. Similar findings are seen in specific stages of platelet activation in collagen-stimulated model. Further evidence obtained from different force measurement region with activated platelets shows that platelet migration is correlated to the more elasticity of pseudopodia while high stiffness at the center region. Overall, ultrastructural and nanomechanical profiling by AFM provides quantitative indicators in the clinical diagnostics of AMI with mechanobiological significance.
authors.affiliation: Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China.
authors.forename: Aiqun
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Analytical and Testing Center, Jinan University, Guangzhou 510632, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Liang
authors.affiliation: Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China; State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau 999078, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China. Electronic address: thhcai@jnu.edu.cn.
authors.forename: Huai-Hong
authors.initials: HH
authors.lastname: Cai
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China; Department of Respiratory Medicine, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address: 948299690@qq.com.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
mesh_terms: D001696:Biomechanical Phenomena; D001777:Blood Coagulation; D001792:Blood Platelets / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D016022:Case-Control Studies; D003094:Collagen / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004548:Elasticity; D018056:Hemorheology; D006801:Humans; D018625:Microscopy, Atomic Force; D009203:Myocardial Infarction / Q000473:pathology*; D015539:Platelet Activation / Q000187:drug effects*; D011554:Pseudopodia / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure
chemical_list: D003094:Collagen
keywords: Acute myocardial infarction; Atomic force microscope; Collagen stimulus; Platelet activation; Stiffness profiles