title: High expression of PINK1 promotes proliferation and chemoresistance of NSCLC.
abstract: PTEN-induced putative kinase 1 (PINK1) was identified initially as a gene upregulated in cancer cells which regulates cellular processes of significance in cancer cell biology, including cell survival, stress resistance and the cell cycle. However, the expression and function of PINK1 in non-small cell lung cancer (NSCLC) has not been determined yet. We demonstrated high PINK1 expression in NSCLC tumor tissues and cell lines as assessed by western blot and immunohistochemistry (IHC) assays. In addition, IHC analysis revealed that PINK1 expression was associated with a more invasive tumor phenotype and poor prognosis. Furthermore, in vitro studies using upregulation and knockdown of PINK1 confirmed that PINK1 promoted cell proliferation of NSCLC, which might be through as the NF-κB pathway. Moreover, we also demonstrated that downregulation of PINK1 enhanced cisplatin (CDDP)-induced NSCLC cell apoptosis. Together, our findings indicate that PINK1 plays a significant role in NSCLC progression and chemoresistance, and highlights its potential role as a target in future anticancer therapies.
authors.affiliation: Department of Respiratory Disease, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Disease, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Rehabilitation, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226200, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ni
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Jieru
authors.initials: J
authors.lastname: Hung
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Intensive Care Unit, Qidong People's Hospital, Nantong, Jiangsu 226200, P.R. China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Disease, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Pathology, Medical College of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ji
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002945:Cisplatin / Q000008:administration & dosage; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D011494:Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D015398:Signal Transduction / Q000235:genetics; D000099202:PTEN-Induced Putative Kinase
chemical_list: D002945:Cisplatin; D011494:Protein Kinases; D000099202:PTEN-Induced Putative Kinase