title: Investigating the effect of available redox protein ratios for the conversion of a steroid by a myxobacterial CYP260A1.
abstract: Since cytochromes P450 are external monooxygenases, available surrogate redox partners have been used to reconstitute the P450 activity. However, the effect of various ratios of P450s and the redox proteins have not been extensively studied so far, although different combinations of the redox partners have shown variations in substrate conversion. To address this issue, CYP260A1 was reconstituted with various ratios of adrenodoxin and adrenodoxin reductase to convert 11-deoxycorticosterone, and the products were characterized by NMR. We show the effect of the available redox protein ratios not only on the P450 catalytic activity but also on the product pattern.
authors.affiliation: Institute of Biochemistry, Saarland University, Saarbrücken, Germany.
authors.forename: Yogan
authors.initials: Y
authors.lastname: Khatri
authors.affiliation: Institute of Biochemistry, Saarland University, Saarbrücken, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Schifrin
authors.affiliation: Institute of Biochemistry, Saarland University, Saarbrücken, Germany.
authors.forename: Rita
authors.initials: R
authors.lastname: Bernhardt
mesh_terms: D000325:Adrenodoxin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D002374:Catalase / Q000378:metabolism; D003900:Desoxycorticosterone / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D009231:Myxococcales / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000378:metabolism; D000072516:Retinoic Acid 4-Hydroxylase / Q000378:metabolism*; D013237:Stereoisomerism; D013250:Steroid Hydroxylases / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: C000621736:11-deoxycorticosterone-1-ene; D001426:Bacterial Proteins; D016166:Free Radical Scavengers; D011994:Recombinant Proteins; D000325:Adrenodoxin; D003900:Desoxycorticosterone; D009249:NADP; D006861:Hydrogen Peroxide; D002374:Catalase; D013250:Steroid Hydroxylases; D000072516:Retinoic Acid 4-Hydroxylase; D013482:Superoxide Dismutase; D005287:Ferredoxin-NADP Reductase; D001205:Ascorbic Acid
keywords: P450; redox protein-ratios; steroid