title: Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection.
abstract: The enzyme-linked immunosorbent assay (ELISA) is a widely employed analytical test used to quantify a given molecule. It relies on the use of specific antibodies, linked to an enzyme, to target the desired molecule. The reaction between the enzyme and its substrate gives rise to the analytical signal that can be quantified. Thanks to their robustness and low cost, molecularly imprinted polymer nanoparticles (nanoMIPs) are a viable alternative to antibodies. Herein, we describe the synthesis of nanoMIPs imprinted for vancomycin and their subsequent application in an ELISA-like format for direct replacement of antibodies.
authors.affiliation: MIP Diagnostics Ltd., University of Leicester, Fielding Johnson Building, University Road, Leicester, LE1 7RH, UK. fc114@leicester.ac.uk.
authors.forename: Francesco
authors.initials: F
authors.lastname: Canfarotta
authors.affiliation: Department of Chemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Smolinska-Kempisty
authors.affiliation: Department of Chemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK.
authors.forename: Sergey
authors.initials: S
authors.lastname: Piletsky
mesh_terms: D000906:Antibodies / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D008958:Models, Molecular; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D014640:Vancomycin / Q000032:analysis*
chemical_list: D000906:Antibodies; D011108:Polymers; D014640:Vancomycin
keywords: Analyte quantification; Assay; Diagnostics; Molecularly imprinted nanoparticles