title: Altered expression of ganglioside GM3 molecular species and a potential regulatory role during myoblast differentiation.
abstract: Gangliosides (sialic acid-containing glycosphingolipids) help regulate many important biological processes, including cell proliferation, signal transduction, and differentiation, via formation of functional microdomains in plasma membranes. The structural diversity of gangliosides arises from both the ceramide moiety and glycan portion. Recently, differing molecular species of a given ganglioside are suggested to have distinct biological properties and regulate specific and distinct biological events. Elucidation of the function of each molecular species is important and will provide new insights into ganglioside biology. Gangliosides are also suggested to be involved in skeletal muscle differentiation; however, the differential roles of ganglioside molecular species remain unclear. Here we describe striking changes in quantity and quality of gangliosides (particularly GM3) during differentiation of mouse C2C12 myoblast cells and key roles played by distinct GM3 molecular species at each step of the process.
authors.affiliation: From the Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai 981-8558, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Go
authors.affiliation: From the Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai 981-8558, Japan.|Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan, and.
authors.forename: Shiori
authors.initials: S
authors.lastname: Go
authors.affiliation: From the Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai 981-8558, Japan.
authors.forename: Lucas
authors.initials: L
authors.lastname: Veillon
authors.affiliation: Department of Medical Biotechnology and Translational Medicine, University of Milan, 20090 Segrate Milano, Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Ciampa
authors.affiliation: Department of Medical Biotechnology and Translational Medicine, University of Milan, 20090 Segrate Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Mauri
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan, and.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sato
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan, and.
authors.forename: Ken
authors.initials: K
authors.lastname: Kitajima
authors.affiliation: Department of Medical Biotechnology and Translational Medicine, University of Milan, 20090 Segrate Milano, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Prinetti
authors.affiliation: Department of Medical Biotechnology and Translational Medicine, University of Milan, 20090 Segrate Milano, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: Sonnino
authors.affiliation: From the Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai 981-8558, Japan, jin@tohoku-mpu.ac.jp.
authors.forename: Jin-Ichi
authors.initials: JI
authors.lastname: Inokuchi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002518:Ceramides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005679:G(M3) Ganglioside / Q000737:chemistry*; D006028:Glycosphingolipids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry; D051379:Mice; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D015398:Signal Transduction
chemical_list: D002518:Ceramides; D005679:G(M3) Ganglioside; D006028:Glycosphingolipids; D008055:Lipids; D019158:N-Acetylneuraminic Acid
keywords: ceramide; differentiation; ganglioside; sialic acid; skeletal muscle