title: Elevated OPRD1 promoter methylation in Alzheimer's disease patients.
abstract: Aberrant DNA methylation has been observed in the patients with Alzheimer's disease (AD), a common neurodegenerative disorder in the elderly. OPRD1 encodes the delta opioid receptor, a member of the opioid family of G-protein-coupled receptors. In the current study, we compare the DNA methylation levels of OPRD1 promoter CpG sites (CpG1, CpG2, and CpG3) between 51 AD cases and 63 controls using the bisulfite pyrosequencing technology. Our results show that significantly higher CpG3 methylation is found in AD cases than controls. Significant associations are found between several biochemical parameters (including HDL-C and ALP) and CpG3 methylation. Subsequent luciferase reporter gene assay shows that DNA fragment containing the three OPRD1 promoter CpGs is able to regulate gene expression. In summary, our results suggest that OPRD1 promoter hypermethylation is associated with the risk of AD.
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Ji
authors.affiliation: Department of Neurology, the 148 Central Hospital of PLA, Zibo, Shandong, China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Guili
authors.initials: G
authors.lastname: Liu
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Chang
authors.affiliation: Ningbo Kangning Hospital, Ningbo, Zhejiang, China.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Ningbo Kangning Hospital, Ningbo, Zhejiang, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Xuting
authors.initials: X
authors.lastname: Xu
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Qingxiao
authors.initials: Q
authors.lastname: Hong
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Liting
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Neurology, the 148 Central Hospital of PLA, Zibo, Shandong, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Internal Medicine for Cadres, the First Affiliated Hospital of Xinjiang Medical University, Urumchi, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Ningbo No. 1 Hospital, Ningbo, Zhejiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Medicine, Lishui University, Lishui, Zhejiang, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Guo
authors.affiliation: The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zha
authors.affiliation: The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Niu
authors.affiliation: The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Weng
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Qinwen
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D019175:DNA Methylation; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017465:Receptors, Opioid, delta / Q000235:genetics*
chemical_list: C529706:OPRD1 protein, human; D017465:Receptors, Opioid, delta