title: Transcriptomic features of Pecten maximus oocyte quality and maturation.
abstract: The king scallop Pecten maximus is a high valuable species of great interest in Europe for both fishery and aquaculture. Notably, there has been an increased investment to produce seed for enhancement programmes of wild scallop populations. However, hatchery production is a relatively new industry and it is still underdeveloped. Major hurdles are spawning control and gamete quality. In the present study, a total of 14 scallops were sampled in the bay of Brest (Brittany, France) to compare transcriptomic profiles of mature oocytes collected by spawning induction or by stripping. To reach such a goal, a microarray analysis was performed by using a custom 8x60K oligonucleotide microarray representing 45,488 unique scallop contigs. First we identified genes that were differentially expressed depending on oocyte quality, estimated as the potential to produce D-larvae. Secondly, we investigated the transcriptional features of both stripped and spawned oocytes. Genes coding for proteins involved in cytoskeletal dynamics, serine/threonine kinases signalling pathway, mRNA processing, response to DNA damage, apoptosis and cell-cycle appeared to be of crucial importance for both oocyte maturation and developmental competence. This study allowed us to dramatically increase the knowledge about transcriptional features of oocyte quality and maturation, as well as to propose for the first time putative molecular markers to solve a major bottleneck in scallop aquaculture.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Padova, Italy.
authors.forename: Marianna
authors.identifier: http://orcid.org/0000-0002-2424-7484
authors.initials: M
authors.lastname: Pauletto
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Padova, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Milan
authors.affiliation: Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de l'Environnement Marin (LEMAR), Plouzané, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Huvet
authors.affiliation: Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de l'Environnement Marin (LEMAR), Plouzané, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Corporeau
authors.affiliation: Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des sciences de l'Environnement Marin (LEMAR), Plouzané, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Suquet
authors.affiliation: Departament de Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona i Institut de Biomedicina de la Universitat de Barcelona, Barcelona, Spain.
authors.forename: Josep V
authors.initials: JV
authors.lastname: Planas
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC), Vigo, Pontevedra, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Moreira
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC), Vigo, Pontevedra, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Figueras
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC), Vigo, Pontevedra, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Novoa
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Padova, Italy.
authors.forename: Tomaso
authors.initials: T
authors.lastname: Patarnello
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Padova, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Bargelloni
mesh_terms: D000818:Animals; D005260:Female; D005298:Fertility; D020869:Gene Expression Profiling; D007814:Larva / Q000235:genetics; D008297:Male; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D049910:Pecten / Q000235:genetics* / Q000254:growth & development*; D014158:Transcription, Genetic