title: First Report of the Carbapenemase GeneblaOXA-499in Acinetobacter pittii.
abstract: We identified the carbapenemase geneblaOXA-499, a variant ofblaOXA-143, from a clinical isolate ofAcinetobacter pittiifor the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptibleAcinetobacterspp., we were able to show that OXA-499 confers resistance to carbapenems.
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Roshan
authors.initials: R
authors.lastname: D'Souza
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.|Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, South Korea.
authors.forename: Naina Adren
authors.initials: NA
authors.lastname: Pinto
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany, Germany.|German Center for Infection Research (DZIF), Partner Site, Bonn-Cologne, Germany.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Higgins
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.|Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, South Korea.
authors.forename: Insik
authors.initials: I
authors.lastname: Hwang
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea deyong@yuhs.ac.
authors.forename: Dongeun
authors.initials: D
authors.lastname: Yong
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Jongrak
authors.initials: J
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Kyoungwon
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; D001618:beta-Lactamases; C577374:beta-lactamase OXA-143, Acinetobacter baumannii; C000625760:beta-lactamase OXA-499, Acinetobacter pittii; C063912:carbapenemase
keywords: Acinetobacter pittii; OXA-143-like; OXA-499; carbapenemase resistant