title: Repair of Osteochondral Defects Using Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells in a Rabbit Model.
abstract: Umbilical cord Wharton's jelly-derived mesenchymal stem cell (WJMSC) is a new-found mesenchymal stem cell in recent years with multiple lineage potential. Due to its abundant resources, no damage procurement, and lower immunogenicity than other adult MSCs, WJMSC promises to be a good xenogenous cell candidate for tissue engineering. This in vivo pilot study explored the use of human umbilical cord Wharton's jelly mesenchymal stem cells (hWJMSCs) containing a tissue engineering construct xenotransplant in rabbits to repair full-thickness cartilage defects in the femoral patellar groove. We observed orderly spatial-temporal remodeling of hWJMSCs into cartilage tissues during repair over 16 months, with characteristic architectural features, including a hyaline-like neocartilage layer with good surface regularity, complete integration with adjacent host cartilage, and regenerated subchondral bone. No immune rejection was detected when xenograft hWJMSCs were implanted into rabbit cartilage defects. The repair results using hWJMSCs were superior to those of chondrogenically induced hWJMSCs after assessing gross appearance and histological grading scores. These preliminary results suggest that using novel undifferentiated hWJMSCs as seed cells might be a better approach than using transforming growth factor-β-induced differentiated hWJMSCs for in vivo tissue engineering treatment of cartilage defects. hWJMSC allografts may be promising for clinical applications.
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Shuyun
authors.identifier: 0000-0003-3865-4547
authors.initials: S
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China; Department of Orthopaedics, The 251st Hospital of Chinese PLA, Zhangjiakou 075000, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Guo
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Huang
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jiang
authors.identifier: 0000-0003-4662-9288
authors.initials: J
authors.lastname: Peng
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xu
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Shibi
authors.initials: S
authors.lastname: Lu
authors.affiliation: Beijing Key Laboratory of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Quanyi
authors.identifier: 0000-0001-5542-4079
authors.initials: Q
authors.lastname: Guo
mesh_terms: D064591:Allografts; D000818:Animals; D002356:Cartilage / Q000293:injuries / Q000473:pathology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival; D020219:Chondrogenesis; D004195:Disease Models, Animal; D006801:Humans; D007107:Immune System; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D051381:Rats; D011923:Rats, Nude; D023822:Tissue Engineering / Q000379:methods; D014470:Umbilical Cord / Q000166:cytology*; D059631:Wharton Jelly / Q000166:cytology*; D014945:Wound Healing