title: Selection of developmentally competent human oocytes aspirated during cesarean section.
abstract: OBJECTIVE
  To evaluate the efficiency/safety of Brilliant Cresyl Blue (BCB) staining as a selection method of developmentally competent immature human oocytes.
  
  
  METHODS
  Immature oocytes of 32 pregnant women were recovered during cesarean section (CS). After retrieval, 92 oocytes were randomly divided into two groups: control (directly disposed to in vitro maturation - IVM) and treated - exposed to BCB 26 μM during 60 min. After staining, the treated group was classified as cytoplasm coloration, BCB positive (blue) or negative (colorless), and then disposed to IVM. Nuclear status was checked after 24 and 48 h of IVM. Nuclear maturation (polar body extrusion), meiosis resumption (absence of germinal vesicle) and degeneration rates were evaluated among the three groups (control, BCB positive and BCB negative) using Generalized Estimating Equations, followed by Bonferroni's correction for multiple comparisons.
  
  
  RESULTS
  Nuclear maturation was higher in BCB positive compared to BCB negative, after 24 and 48 h of IVM (p = .004 and p = .032). The control group was equal to BCB positive. There was no difference among groups analyzing meiosis resumption and degeneration rates.
  
  
  CONCLUSIONS
  The BCB test can be a good marker in pre-selection procedures of developmentally competent human oocytes aspirated during CS.
authors.affiliation: a Instituto de Ciências Básicas da Saúde (ICBS) , Laboratório de Biologia Molecular Endócrina e Tumoral - Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre , RS , Brazil.
authors.forename: Diego
authors.identifier: http://orcid.org/0000-0003-0702-6994
authors.initials: D
authors.lastname: Duarte Alcoba
authors.affiliation: b Serviço de Ginecologia e Obstetrícia - Hospital de Clínicas de Porto Alegre (HCPA) , Rua Ramiro Barcelos , Porto Alegre , Rio Grande do Sul (RS) , Brazil.
authors.forename: Edimárlei
authors.initials: E
authors.lastname: Gonsales Valério
authors.affiliation: a Instituto de Ciências Básicas da Saúde (ICBS) , Laboratório de Biologia Molecular Endócrina e Tumoral - Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre , RS , Brazil.
authors.forename: Maiara
authors.initials: M
authors.lastname: Conzatti
authors.affiliation: a Instituto de Ciências Básicas da Saúde (ICBS) , Laboratório de Biologia Molecular Endócrina e Tumoral - Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre , RS , Brazil.
authors.forename: Júlia
authors.initials: J
authors.lastname: Schneider
authors.affiliation: b Serviço de Ginecologia e Obstetrícia - Hospital de Clínicas de Porto Alegre (HCPA) , Rua Ramiro Barcelos , Porto Alegre , Rio Grande do Sul (RS) , Brazil.|c Department of Gynecological Endocrinology and Reproductive Medicine , University Hospital Heidelberg , Heidelberg , Germany.
authors.forename: Edison
authors.initials: E
authors.lastname: Capp
authors.affiliation: b Serviço de Ginecologia e Obstetrícia - Hospital de Clínicas de Porto Alegre (HCPA) , Rua Ramiro Barcelos , Porto Alegre , Rio Grande do Sul (RS) , Brazil.
authors.forename: Helena
authors.initials: H
authors.lastname: von Eye Corleta
authors.affiliation: a Instituto de Ciências Básicas da Saúde (ICBS) , Laboratório de Biologia Molecular Endócrina e Tumoral - Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre , RS , Brazil.
authors.forename: Ilma Simoni
authors.initials: IS
authors.lastname: Brum
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002585:Cesarean Section; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D054315:Oocyte Retrieval / Q000379:methods*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010078:Oxazines; D011247:Pregnancy; D011897:Random Allocation; D013194:Staining and Labeling; D013997:Time Factors; D055815:Young Adult
chemical_list: D010078:Oxazines; C006797:Brilliant Cresyl Blue; D005954:Glucosephosphate Dehydrogenase
keywords: Brilliant Cresyl Blue; In vitro maturation; cesarean section; fertility; oocyte selection