title: Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET.
abstract: Over the last decade, advancements in the time and space resolution of microscopy technologies have enabled dissection of the molecular events involved in T cell Immunological Synapse (IS) formation. Using a combination of Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imagining Microscopy (FLIM), we have demonstrated dynamic plasma membrane binding by cytoplasmic domains of T cell receptor (TCR)-associated CD3 chains and other T cell transmembrane receptors. We have developed methods for imaging such membrane binding both at steady state and during receptor triggering at the IS. Plasma membrane binding by cytoplasmic domains may represent a novel mechanism for regulating the signaling function of important receptors in the immune system.
authors.affiliation: Department of Microbiology and Immunology, Institute for Research in Immunology and Cancer, Université de Montréal, PO Box 6128, Downtown Station, Montreal, Canada, H3C 3J7. etienne.gagnon@umontreal.ca.
authors.forename: Etienne
authors.initials: E
authors.lastname: Gagnon
authors.affiliation: Department of Microbiology and Immunology, Institute for Research in Immunology and Cancer, Université de Montréal, PO Box 6128, Downtown Station, Montreal, Canada, H3C 3J7.
authors.forename: Audrey
authors.initials: A
authors.lastname: Connolly
authors.affiliation: Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA, 02115, USA.|Program in Immunology, Harvard Medical School, Boston, MA, 02115, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Dobbins
authors.affiliation: Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA, 02115, USA.|Program in Immunology, Harvard Medical School, Boston, MA, 02115, USA.|Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, 02115, USA.
authors.forename: Kai W
authors.initials: KW
authors.lastname: Wucherpfennig
mesh_terms: D017252:CD3 Complex / Q000276:immunology*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D054992:Immunological Synapses / Q000276:immunology*; D019169:Jurkat Cells; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell
keywords: FLIM; FRET; Immunological synapse; Lipid bilayers; Membrane binding; TCR-CD3 complex