title: [Effects of miR-382 on cell migration, invasion and proliferation of gastric cancer cell lines MGC-803].
abstract: Objective:To investigate the effects of miR-382 on cell migration, invasion and proliferation of gastric cancer cell lines MGC-803.Methods:The level of miR-382 expression was detected by real-time RT-PCR in 50 paired gastric cancer tissues and their adjacent normal tissues. miR-382 overexpression was achieved by transfection of construct pcDNA-miR-382 into MGC-803 cells. The migration, invasion and proliferation of MGC-803 cells were detected by the scratch wound assay, Transwell and CCK-8, respectively.Results:miR-382 was decreased in 41 cases (82%) of gastric cancer tissues compared to their control. Furthermore, overexpression of miR-382 effectively inhibited the migration, invasion and proliferation of MGC-803 cells(P<0.05).Conclusion:Down-regulation of miR-382 has a correlation with the progression of gastric cancer. Up-regulation of miR-382 can inhibit the migration, invasion and proliferation of MGC-803 cells.
authors.affiliation: Department of VIP, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Bu
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: X L
authors.initials: XL
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Xu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs; D009361:Neoplasm Invasiveness; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms; D014162:Transfection; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs
keywords: Gastric cancer; Invasion; Migration; Proliferation; miR-382