title: Structural Pharmacokinetics of Polymethoxylated Flavones in Rat Plasma Using HPLC-MS/MS.
abstract: The purpose of this study was to investigate the pharmacokinetics of the polymethoxylated flavonoids kumatakenin, pachypodol, and retusin, which contain two, three, or four methoxy substitutions, using a validated ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method in rats. The pharmacokinetic results demonstrated that the elimination half-lives for kumatakenin, pachypodol, and retusin were 30 ± 11.6, 39.4 ± 19.5, and 106.9 ± 26 min, respectively, for the low dose group and 54.5 ± 16.5, 33.8 ± 10, and 134.6 ± 34.7 min for the high dose group. The results suggested that the area under the curve values (AUC) for the analytes did not correlate with the number of methoxy groups. Pachypodol had the lowest AUC, which may have been correlated with lipophilicity, for both the low and high dose groups. In conclusion, the polymethoxylated flavonoid pachypodol is more hydrophilic than kumatakenin or retusin, which were correlated with the pharmacokinetic results.
authors.affiliation: Institute of Traditional Medicine, National Yang-Ming University , Taipei 112, Taiwan.
authors.forename: Jing-Ting
authors.initials: JT
authors.lastname: Huang
authors.affiliation: Institute of Traditional Medicine, National Yang-Ming University , Taipei 112, Taiwan.
authors.forename: Yung-Yi
authors.identifier: http://orcid.org/0000-0001-7473-2835
authors.initials: YY
authors.lastname: Cheng
authors.affiliation: National Research Institute of Chinese Medicine, Ministry of Health and Welfare , Taipei 112, Taiwan.
authors.forename: Lie-Chwen
authors.identifier: http://orcid.org/0000-0001-9518-9018
authors.initials: LC
authors.lastname: Lin
authors.affiliation: Institute of Traditional Medicine, National Yang-Ming University , Taipei 112, Taiwan.|School of Pharmacy, College of Pharmacy, Kaohsiung Medical University , Kaohsiung 807, Taiwan.|Department of Chemical Engineering, National United University , Miaoli 36063, Taiwan.
authors.forename: Tung-Hu
authors.identifier: http://orcid.org/0000-0002-9007-2547
authors.initials: TH
authors.lastname: Tsai
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047309:Flavones / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D047309:Flavones
keywords: UHPLC-MS/MS; kumatakenin; pachypodol; pharmacokinetics; polymethoxylated flavonoids; rat plasma; retusin