title: [Biomarkers of prostate cancer sensitivity to the Sendai virus].
abstract: Metastatic prostate cancer is often associated with either primary or intractable castration-resistant prostate cancer (CRPC), thus justifying the search for entirely new ways of treatment. Oncolytic viruses are able to selectively induce the death of tumor cells without affecting normal cells. A murine Sendai virus has potential to be used as an oncolytic agent. However, tumors vary in their sensitivity to different viruses, prompting us to attempt to identify corresponding biomarkers that reflect the interaction of cancer cells and the virus. Here, we show that the sensitivity of primary prostatic adenocarcinoma cell lines to Sendai virus strain (SeVM) vary substantially. Using quantitative PCR, we evaluated expression levels of genes that encode RIG-1-like and Toll-like receptors (TLRs) in cell lines and showed that the levels of mRNAs that encode TLR3 and TLR7 correlate with a degree of sensitivity of the cells to Sendai virus. The lines with lower levels of TLR3 and TLR7 expression are more sensitive to the virus.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Belova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.|Pirogov Russian National Research Medical University, Moscow, 117997 Russia.
authors.forename: A O
authors.initials: AO
authors.lastname: Sosnovtseva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.|lipatovaanv@gmail.com.
authors.forename: A V
authors.initials: AV
authors.lastname: Lipatova
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: K M
authors.initials: KM
authors.lastname: Njushko
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Volchenko
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Belyakov
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Sudalenko
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Krasheninnikov
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: P V
authors.initials: PV
authors.lastname: Shegai
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.|National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: A F
authors.initials: AF
authors.lastname: Sadritdinova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Fedorova
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Vorobjov
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: B Y
authors.initials: BY
authors.lastname: Alekseev
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Kaprin
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.|National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, 125284 Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Kudryavtseva
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis; D050504:Oncolytic Viruses; D064129:Prostatic Neoplasms, Castration-Resistant / Q000235:genetics / Q000628:therapy*; D029082:Sendai virus; D051196:Toll-Like Receptor 3 / Q000235:genetics; D051199:Toll-Like Receptor 7 / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C495344:TLR3 protein, human; C495348:TLR7 protein, human; D051196:Toll-Like Receptor 3; D051199:Toll-Like Receptor 7
keywords: Sendai virus; gene expression; oncolytic viruses; primary cell lines; prostate cancer; quantitative PCR