title: Effects of the Inoculant Strain Pseudomonas sp. SPN31 nah+and of 2-Methylnaphthalene Contamination on the Rhizosphere and Endosphere Bacterial Communities of Halimione portulacoides.
abstract: The aim of this study is to evaluate the effects of the inoculation of the saltmarsh plant (Halimione portulacoides) with Pseudomonas sp. SPN31 nah+ combined with exposure to 2-methylnaphthalene (2-MtN) on the plant rhizosphere and endosphere bacterial communities as well as on plant health. To achieve this goal, microcosm experiments were set up. Denaturing gradient gel electrophoresis (DGGE) profiles and statistical analysis showed that rhizosphere and endosphere bacterial communities had distinct responses to plant inoculation and/or exposure to 2-MtN. PCR-sequencing analysis of nah genes encoding for 2-MtN degrading enzymes suggested the presence of Pseudomonas sp. SPN31 nah+ in the endosphere of H. portulacoides with 2-MtN contamination. Moreover, a significant effect in the photosynthetic performance of inoculated plants was detected. To conclude, despite the potential beneficial effect of plant inoculation with Pseudomonas sp. SPN31 nah+ endophytic bacteria may have on plant health, no significant effect on the removal of MtN was detected for the level of contamination used in the study.
authors.affiliation: Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Oliveira
authors.affiliation: Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Newton C M
authors.initials: NC
authors.lastname: Gomes
authors.affiliation: Department of Chemistry and QOPNA, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Magda
authors.initials: M
authors.lastname: Santos
authors.affiliation: Department of Chemistry and QOPNA, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Adelaide
authors.initials: A
authors.lastname: Almeida
authors.affiliation: Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Lillebø
authors.affiliation: Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Ezequiel
authors.affiliation: Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Serôdio
authors.affiliation: Department of Chemistry and QOPNA, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Artur M S
authors.initials: AM
authors.lastname: Silva
authors.affiliation: Department of Chemistry and QOPNA, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Mário M Q
authors.initials: MM
authors.lastname: Simões
authors.affiliation: Department of Chemistry and QOPNA, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Sílvia M
authors.initials: SM
authors.lastname: Rocha
authors.affiliation: Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal. acunha@ua.pt.
authors.forename: Ângela
authors.identifier: http://orcid.org/0000-0002-9118-3521
authors.initials: Â
authors.lastname: Cunha
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics; D001673:Biodegradation, Environmental; D019663:Chenopodiaceae / Q000187:drug effects* / Q000382:microbiology* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D009281:Naphthalenes / Q000009:adverse effects* / Q000378:metabolism; D010788:Photosynthesis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D011549:Pseudomonas / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000009:adverse effects* / Q000378:metabolism; D055549:Volatile Organic Compounds
chemical_list: D009281:Naphthalenes; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D055549:Volatile Organic Compounds; C027384:2-methylnaphthalene