title: Label-Free Biosensors Based on Bimodal Waveguide (BiMW) Interferometers.
abstract: The bimodal waveguide (BiMW) sensor is a novel common path interferometric transducer based on the evanescent field detection principle, which in combination with a bio-recognition element allows the direct detection of biomolecular interactions in a label-free scheme. Due to its inherent high sensitivity it has great potential to become a powerful analytical tool for monitoring substances of interest in areas such as environmental control, medical diagnostics and food safety, among others. The BiMW sensor is fabricated using standard silicon-based technology allowing cost-effective production, and meeting the requirements of portability and disposability necessary for implementation in a point-of-care (POC) setting.In this chapter we describe the design and fabrication of the BiMW transducer, as well as its application for bio-sensing purposes. We show as an example the biosensor capabilities two different applications: (1) the immunodetection of Irgarol 1051 biocide useful in the environmental field, and (2) the detection of human growth hormone as used in clinical diagnostics. The detection is performed in real time by monitoring changes in the intensity pattern of light exiting the BiMW transducer resulting from antigen-antibody interactions on the surface of the sensor.
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, The Barcelona Institute of Science and Technology, 08193, Bellaterera, Barcelona, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Herranz
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, The Barcelona Institute of Science and Technology, 08193, Bellaterera, Barcelona, Spain.
authors.forename: Adrián Fernández
authors.initials: AF
authors.lastname: Gavela
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, The Barcelona Institute of Science and Technology, 08193, Bellaterera, Barcelona, Spain. laura.lechuga@icn2.cat.|CIBER-BBN, Campus UAB, Ed-ICN2, Bellaterera, 08193, Barcelona, Spain. laura.lechuga@icn2.cat.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Lechuga
mesh_terms: D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D019382:Human Growth Hormone / Q000032:analysis / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D019095:Point-of-Care Systems; D012031:Refractometry / Q000379:methods; D012680:Sensitivity and Specificity; D013223:Statistics as Topic / Q000379:methods
chemical_list: D000906:Antibodies; D019382:Human Growth Hormone
keywords: Bimodal waveguide interferometry; Biofunctionalization; Biorecognition; Evanescent field biosensor; Immunoassay; Integrated optics