title: Regulation function of MMP-1 downregulated by siRNA on migration of heat-denatured dermal fibroblasts.
abstract: Cutaneous wound healing is a complex physiological process that requires the efforts of various cell types and signaling pathways and often results in thickened collagen-enriched healed tissue called a scar. Therefore, the identification of the mechanism of cutaneous wound healing is necessary and has great value in providing better treatment. Here, we demonstrated that MMP-1 inhibition could promote cell proliferation in dermal fibroblasts via the MTT assay. Meanwhile, we investigated cell migration by flow cytometry and tested type I collagenase activity. We found that MMP-1 inhibition promoted cell proliferation and inhibited cell migration and type I collagenase activity. In conclusion, our study demonstrated that MMP-1 might be a potential therapeutic target in cutaneous wound healing.
authors.affiliation: a Department of Burn , Ningbo No. 2 Hospital, Ningbo , China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: He
authors.affiliation: b Department of Clinical Laboratory , Ningbo No. 2 Hospital, Ningbo , China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Dai
authors.affiliation: a Department of Burn , Ningbo No. 2 Hospital, Ningbo , China.
authors.forename: Youfen
authors.initials: Y
authors.lastname: Fan
authors.affiliation: a Department of Burn , Ningbo No. 2 Hospital, Ningbo , China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: c Key Laboratory for Green Chemical Process of Ministry of Education , School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology , Wuhan , China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Zhao
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D017364:Collagenases / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology*
chemical_list: D034741:RNA, Small Interfering; D017364:Collagenases; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: MMP-1; TIMP; heat-denatured dermal fibroblast; migration; siRNA