title: Prognostic significance of CD133 and ezrin expression in colorectal carcinoma.
abstract: Despite the improvement in the diagnostic and the therapeutic modalities, colorectal cancer (CRC) morbidity and mortality remain high in both the developed and the developing countries. So, we are in a need to recognize new efficient diagnostic and prognostic biomarkers of CRC. That may help in providing individualized targeted therapy for this lethal malignancy. In this study, we investigated the expression and the prognostic significance of CD133 and ezrin mRNA and protein by quantitative RT-PCR and immunohistochemical analysis in primary CRC cases and the surrounding normal colonic mucosa. The correlations between the expression of both markers and clinicopathological parameters were performed. Compared to control, the expression of CD133 and ezrin mRNA and protein in CRC were significantly up-regulated (P < 0.05). In addition, a strong positive correlation between both markers was found (r = 0.867, P < 0.001). Moreover, elevated levels of both markers were significantly associated with the stage, lymph node involvement, distant metastasis, and recurrence at both mRNA and protein levels (P < 0.05). In conclusion, there is a clinical significance of CD133 and ezrin as potential biomarkers for predicting CRC patients of aggressive behavior and poor prognosis. © 2017 IUBMB Life, 69(5):328-340, 2017.
authors.affiliation: Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Anan
authors.initials: A
authors.lastname: Fathi
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Hala
authors.initials: H
authors.lastname: Mosaad
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Samia
authors.initials: S
authors.lastname: Hussein
authors.affiliation: Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Mona
authors.initials: M
authors.lastname: Roshdy
authors.affiliation: Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Eman I
authors.initials: EI
authors.lastname: Ismail
mesh_terms: D000071916:AC133 Antigen / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D015854:Up-Regulation; D000099149:Ezrin
chemical_list: D000071916:AC133 Antigen; D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; D000099149:Ezrin; C000606023:PROM1 protein, human
keywords: CD133; RT-PCR; biomarkers; colorectal cancer; ezrin; immunohistochemistry; prognosis