title: Comparison of in vitro dentinal tubule occluding efficacy of two different methods using a nano-scaled bioactive glass-containing desensitising agent.
abstract: OBJECTIVE
  This study aimed to compare the in vitro dentinal tubule occluding efficacy of two different methods using a nano-scaled bioactive glass (BG)-containing desensitising agent.
  
  
  METHODS
  Citric acid treated dentine discs were randomly divided into 7 groups (n=8). Group A1, A2 and A3: dentine discs coated with BG desensitising paste; Group B1, B2 and B3: dentine discs coated with BG desensitising paste and covered with matched transparent trays; and control group (GroupC): dentine discs treated with deionised water. Field Emission Scanning Electron Microscopy (FE-SEM) was used to capture topographical images of each dentine discs after they were immersed in artificial saliva for corresponding treatment time and dentinal tubules exposure rates were thus measured. Elemental compositions of dentine discs were identified using Energy-Dispersive X-ray Spectroscopy (EDX).
  
  
  RESULTS
  FE-SEM revealed better tubule occluding effects in Group B. Dentinal tubules in Group B3 were totally occluded with continuous homogeneous minerals to a depth of 20.6-24.7μm. Dentinal tubule exposure rates in Group B1, Group B2 and Group B3 were lower than that in Group A1, Group A2 and Group A3. EDX indicated that occluding deposits observed in each group were calcium-deficient hydroxyapatite.
  
  
  CONCLUSIONS
  The application of transparent trays in combination with nano-scaled BG-containing desensitising paste could increase the dentinal tubule occluding effectiveness of the latter one and shorten the treatment time.
  
  
  CONCLUSIONS
  Transparent trays could be used in combination with BGs-containing desensitising paste as the containers of the latter one in order to increase the dentinal tubule occluding effectiveness. This could lead to the development of a new therapeutic technique for treating dentine hypersensitivity.
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, PR China; Department of Polyclinics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, PR China; Department of Polyclinics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Tianda
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Stomatology, Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Meng
authors.affiliation: College of Stomatology, Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Stomatology, Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Huaying
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Stomatology, Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Dongjing
authors.initials: D
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, PR China; Department of Polyclinics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, PR China. Electronic address: yaming_chen@njmu.edu.cn.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D019343:Citric Acid / Q000009:adverse effects; D003188:Composite Resins / Q000737:chemistry; D003804:Dentin / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D003806:Dentin Permeability / Q000187:drug effects; D003807:Dentin Sensitivity / Q000188:drug therapy; D004338:Drug Combinations; D005898:Glass / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third; D053758:Nanoparticles / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D014100:Toothpastes / Q000737:chemistry / Q000494:pharmacology; D055815:Young Adult
chemical_list: D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D057133:Dentin Desensitizing Agents; D004338:Drug Combinations; D011140:Polyurethanes; D012464:Saliva, Artificial; D014100:Toothpastes; D019343:Citric Acid
keywords: Bioactive glass; Bioglass; Dentinal tubule occlusion; Dentine hypersensitivity; Desensitising paste; Transparent tray