title: Understanding Host-Pathogen Interactions with Expression Profiling of NILs Carrying Rice-Blast ResistancePi9Gene.
abstract: Magnaporthe oryzaeinfection causes rice blast, a destructive disease that is responsible for considerable decrease in rice yield. Development of resistant varieties via introgressing resistance genes with marker-assisted breeding can eliminate pesticide use and minimize crop losses. Here, resistant near-isogenic line (NIL) of Pusa Basmati-1(PB1) carrying broad spectrum rice blast resistance genePi9was used to investigatePi9-mediated resistance response. Infected and uninfected resistant NIL and susceptible control line were subjected to RNA-Seq. With the exception of one gene (Pi9), transcriptional signatures between the two lines were alike, reflecting basal similarities in their profiles. Resistant and susceptible lines possessed 1043 (727 up-regulated and 316 down-regulated) and 568 (341 up-regulated and 227 down-regulated) unique and significant differentially expressed loci (SDEL), respectively. Pathway analysis revealed higher transcriptional activation of kinases, WRKY, MYB, and ERF transcription factors, JA-ET hormones, chitinases, glycosyl hydrolases, lipid biosynthesis, pathogenesis and secondary metabolism related genes in resistant NIL than susceptible line. Singular enrichment analysis demonstrated that blast resistant NIL is significantly enriched with genes for primary and secondary metabolism, response to biotic stimulus and transcriptional regulation. The co-expression network showed proteins of genes in response to biotic stimulus interacted in a manner unique to resistant NIL uponM. oryzaeinfection. These data suggest thatPi9modulates genome-wide transcriptional regulation in resistant NIL but not in susceptible PB1. We successfully used transcriptome profiling to understand the molecular basis ofPi9-mediated resistance mechanisms, identified potential candidate genes involved in early pathogen response and revealed the sophisticated transcriptional reprogramming during rice-M. oryzaeinteractions.
authors.affiliation: ICAR-National Research Centre on Plant BiotechnologyNew Delhi, India; Department of Bioscience & Biotechnology, Banasthali UniversityTonk, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Jain
authors.affiliation: ICAR-National Research Centre on Plant BiotechnologyNew Delhi, India; Department of Bioscience & Biotechnology, Banasthali UniversityTonk, India.
authors.forename: Pankaj K
authors.initials: PK
authors.lastname: Singh
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: Ritu
authors.initials: R
authors.lastname: Kapoor
authors.affiliation: ICAR-Indian Agricultural Research Institute New Delhi, India.
authors.forename: Apurva
authors.initials: A
authors.lastname: Khanna
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: Amolkumar U
authors.initials: AU
authors.lastname: Solanke
authors.affiliation: ICAR-Indian Agricultural Research Institute New Delhi, India.
authors.forename: S Gopala
authors.initials: SG
authors.lastname: Krishnan
authors.affiliation: ICAR-Indian Agricultural Research Institute New Delhi, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Bioscience & Biotechnology, Banasthali University Tonk, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Sharma
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: Tilak R
authors.initials: TR
authors.lastname: Sharma
keywords: Magnaporthe oryzae; RNA-Seq; WRKY; ethylene; host-pathogen interaction; jasmonic acid; near isogenic lines; rice blast