title: A Broad-Host-Range Tailocin from Burkholderia cenocepacia.
abstract: TheBurkholderia cepaciacomplex (Bcc) consists of 20 closely related Gram-negative bacterial species that are significant pathogens for persons with cystic fibrosis (CF). Some Bcc strains are highly transmissible and resistant to multiple antibiotics, making infection difficult to treat. A tailocin (phage tail-like bacteriocin), designated BceTMilo, with a broad host range against members of the Bcc, was identified inB. cenocepaciastrain BC0425. Sixty-eight percent of Bcc representing 10 species and 90% of non-BccBurkholderiastrains tested were sensitive to BceTMilo. BceTMilo also showed killing activity againstPseudomonas aeruginosaPAO1 and derivatives. Liquid chromatography-mass spectrometry analysis of the major BceTMilo proteins was used to identify a 23-kb tailocin locus in a draft BC0425 genome. The BceTMilo locus was syntenic and highly similar to a 24.6-kb region on chromosome 1 ofB. cenocepaciaJ2315 (BCAL0081 to BCAL0107). A close relationship and synteny were observed between BceTMilo andBurkholderiaphage KL3 and, by extension, with paradigm temperate myophage P2. Deletion mutants in the gene cluster encoding enzymes for biosynthesis of lipopolysaccharide (LPS) in the indicator strainB. cenocepaciaK56-2 conferred resistance to BceTMilo. Analysis of the defined mutants in LPS biosynthetic genes indicated that an α-d-glucose residue in the core oligosaccharide is the receptor for BceTMilo.IMPORTANCEBceTMilo, presented in this study, is a broad-host-range tailocin active againstBurkholderiaspp. As such, BceTMilo and related or modified tailocins have potential as bactericidal therapeutic agents against plant- and human-pathogenicBurkholderia.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, USA.|Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
authors.forename: Guichun W
authors.initials: GW
authors.lastname: Yao
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, USA.|Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
authors.forename: Iris
authors.initials: I
authors.lastname: Duarte
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, USA.|Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
authors.forename: Tram T
authors.initials: TT
authors.lastname: Le
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Carmody
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: LiPuma
authors.affiliation: Center for Phage Technology, Texas A&M University, College Station, Texas, USA.|Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.
authors.forename: Ry
authors.initials: R
authors.lastname: Young
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, USA cf-gonzalez@tamu.edu.|Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Gonzalez
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D057508:Burkholderia cenocepacia / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D042602:Burkholderia cepacia complex / Q000187:drug effects* / Q000254:growth & development; D016680:Genome, Bacterial; D016679:Genome, Viral; D058507:Host Specificity; D006801:Humans; D013058:Mass Spectrometry; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins
keywords: Burkholderia cenocepacia; LPS; broad host range; receptor; tailocin