title: In vitro activity of a partially purified and characterized bark extract of Castanea sativa Mill. (ENC®) against Chlamydia spp.
abstract: Castanea sativa Mill (ENC®), containing tannins against 33 Chlamydia strains, was compared to SMAP-29 with inhibitory effect against C. trachomatis and C. pneumoniae. The ENC® activity against Chlamydia spp. was evaluated determining the lowest concentration to achieve more than half reduction of intact chlamydial inclusions versus controls. ENC® reduced all Chlamydia strains tested at 1 µg/mL, while SMAP-29 induced reductions of C. trachomatis and C. pneumoniae infectivity at 10 µg/mL. A great reduction of C. trachomatis, C. pneumoniae, and C. abortus infectivity was achieved with a 10 µg/mL ENC® concentration, whereas their infectivity was almost inhibited at 100 µg/mL ENC® concentration.
authors.affiliation: a Biomedical and Neuromotor Sciences Department, Pathology , University of Bologna , Bologna , Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Papa
authors.affiliation: b Experimental Diagnostic and Specialty Medicine Department, Microbiology , University of Bologna , Bologna , Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Ginocchietti
authors.affiliation: c Pharmacy and Biotechnology Department , University of Bologna , Bologna , Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Budriesi
authors.affiliation: c Pharmacy and Biotechnology Department , University of Bologna , Bologna , Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Micucci
authors.affiliation: a Biomedical and Neuromotor Sciences Department, Pathology , University of Bologna , Bologna , Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Costa
authors.affiliation: b Experimental Diagnostic and Specialty Medicine Department, Microbiology , University of Bologna , Bologna , Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Biondi
authors.affiliation: b Experimental Diagnostic and Specialty Medicine Department, Microbiology , University of Bologna , Bologna , Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Cevenini
authors.affiliation: b Experimental Diagnostic and Specialty Medicine Department, Microbiology , University of Bologna , Bologna , Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Chiarini
authors.affiliation: c Pharmacy and Biotechnology Department , University of Bologna , Bologna , Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Aldini
authors.affiliation: b Experimental Diagnostic and Specialty Medicine Department, Microbiology , University of Bologna , Bologna , Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Donati
authors.affiliation: b Experimental Diagnostic and Specialty Medicine Department, Microbiology , University of Bologna , Bologna , Italy.
authors.forename: Gian Matteo
authors.initials: GM
authors.lastname: Pollini
authors.affiliation: a Biomedical and Neuromotor Sciences Department, Pathology , University of Bologna , Bologna , Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Cenacchi
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002460:Cell Line; D002689:Chlamydia / Q000187:drug effects* / Q000648:ultrastructure; D066298:In Vitro Techniques; D008253:Macaca mulatta; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts
keywords: Antimicrobial activity; cathelicidin peptide; chlamydiae; hydrolysable tannins