title: The effect of increasing the pore size of nanofibrous scaffolds on the osteogenic cell culture using a combination of sacrificial agent electrospinning and ultrasonication.
abstract: One of the major problems associated with the electrospun scaffolds is their small pore size, which limits the cellular infiltration for bone tissue engineering. In this study, the effect of increasing the pore size on cellular infiltration was studied in poly/nanohydroxyapatite electrospun scaffolds, which were modified using ultrasonication, co-electrospinning with poly (ethylene oxide), and a combination of both. Ultrasonic process was optimized by central composite design. The ultrasonic output power and time of the process were considered as the effective parameters. The pore size of the scaffolds was evaluated by scanning electron microscope. The optimum conditions, according to the pore area and mechanical properties of the scaffolds were selected, and finally the groups that had the highest pore size and mechanical strength were selected for the combined method. Increasing the pore size enhanced the cellular proliferation, extension and infiltration, as well as the osteodifferentiation of stem cells. At the optimum condition, the average cellular infiltration was 36.51 µm compared to the control group with no cellular infiltration. In addition, alkaline phosphatase activity and the expression of osteocalcin and collagen I (COL I) were, respectively, 1.86, 2.54, and 2.16 fold compared to the control group on day 14. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1887-1899, 2017.
authors.affiliation: Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mahdiyeh
authors.initials: M
authors.lastname: Aghajanpoor
authors.affiliation: Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Sameereh
authors.initials: S
authors.lastname: Hashemi-Najafabadi
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Mohamadreza
authors.initials: M
authors.lastname: Baghaban-Eslaminejad
authors.affiliation: Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Bagheri
authors.affiliation: Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyyed
authors.initials: S
authors.lastname: Mohammad Mousavi
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Foruogh
authors.initials: F
authors.lastname: Azam Sayyahpour
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D055664:Electrochemical Techniques; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D010012:Osteogenesis; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D000069453:Ultrasonic Waves
keywords: bone tissue engineering; co-electrospinning; electrospinning; pore size; ultrasonication