title: Characterization of Clostridium Species from Food Commodities and Faecal Specimens in Lagos State, Nigeria.
abstract: BACKGROUND
  Food-borne pathogens are a major public health challenge worldwide. These organisms' cause illnesses leading to time loss in the work place and reduced productivity.Clostridium species cause infections through the production of powerful toxins which are responsible for diarrhoea and cramping. Diarrhoeal diseases due to Clostridia are one of the commonest worldwide but have hardly been reported in Nigeria.
  
  
  OBJECTIVE
  This study characterized Clostridium species from food commodities and human faeces in Lagos State MATERIALS AND METHODS: Four hundred and twenty samples comprising food (220) and faecal (200) specimens in Lagos state were included in this study. Isolates obtained were identified using API 20-A and confirmed by Polymerase Chain Reaction assay and 16S rRNA sequencing. The food samples included meat and meat products, ready to drink traditionally prepared milk products, fresh vegetables, canned foods and local honey.
  
  
  RESULTS
  Seventy (16.7%) Clostridium species were identified, 50 from food and 20 from faeces. Majority of the isolates were obtained from vegetables (56%) and meat products (34%). Of the 70 Clostridial species, 38 (54.3%) were C. perfringens, 5 (7.1%) were C. difficile and 2 (2.9%) were C. botulinum. All 38 (100%) strains of C. perfringens possessed alpha (cpa) toxin gene.
  
  
  CONCLUSIONS
  Clostridium species are present in our environment and contaminate food products posing potential risks to consumers. There is therefore a need for these traditionally made street vended foods to be monitored because they are potential sources of food borne pathogens.
authors.affiliation: Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos. Nigeria.
authors.forename: E E
authors.initials: EE
authors.lastname: Chukwu
authors.forename: F T
authors.initials: FT
authors.lastname: Ogunsola
authors.forename: F O
authors.initials: FO
authors.lastname: Nwaokorie
authors.forename: A O
authors.initials: AO
authors.lastname: Coker