title: Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant Escherichia coli.
abstract: Poly(3-hydroxypropionate) (P3HP) is a thermoplastic with great compostability and biocompatibility, and can be produced through several biosynthetic pathways, in which the glycerol pathway achieved the highest P3HP production. However, exogenous supply of vitamin B12 was required to maintain the activity of glycerol dehydratase, resulting in high production cost. To avoid the addition of VB12, we have previously constructed a P3HP biosynthetic route with β-alanine as intermediate, and the present study aimed to improve the P3HP production of this pathway. L-aspartate decarboxylase PanD was found to be the rate-limiting enzyme in the β-alanine pathway firstly. To improve the pathway efficiency, PanD was screened from four different sources (Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, and Corynebacterium glutamicum). And PanD from C. glutamicum was found to have the highest activity, the P3HP production was improved in flask cultivation with this enzyme. To further improve the production, the host strain was screened and the culture condition was optimized. Under optimal conditions, production and content of P3HP reached to 10.2 g/L and 39.1% (wt/wt [cell dry weight]) in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without VB12.
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.|Laboratory of Microbiology and Antimicrobials Substances, Department of Biochemistry, Faculty of Sciences, University of Dschang, Dschang, Cameroon.
authors.forename: Stephen Tamekou
authors.initials: ST
authors.lastname: Lacmata
authors.affiliation: Laboratory of Microbiology and Antimicrobials Substances, Department of Biochemistry, Faculty of Sciences, University of Dschang, Dschang, Cameroon.
authors.forename: Jules-Roger
authors.initials: JR
authors.lastname: Kuiate
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Ding
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
authors.affiliation: Laboratory of Phytoptotection and Valorization of Vegetals Resources, Biotechnology-Nkolbisson Center, Department of Biochemistry, University of Yaounde, Yaounde, Cameroon.
authors.forename: Thaddée
authors.initials: T
authors.lastname: Boudjeko
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Feng
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.|Randian Technology Company Limited, Tianjin, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D002262:Carboxy-Lyases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial; D010957:Plasmids; D011091:Polyesters / Q000378:metabolism*; D011995:Recombination, Genetic; D015091:beta-Alanine / Q000378:metabolism*
chemical_list: D011091:Polyesters; C000600150:poly(3-hydroxypropionate); D015091:beta-Alanine; D002262:Carboxy-Lyases; C019747:aspartate 4-decarboxylase