title: Development of dietary soluble fibres by enzymatic synthesis and assessment of their digestibility in in vitro, animal and randomised clinical trial models.
abstract: The aim was to develop novel fibres by enzymatic synthesis, to determine their total dietary fibre by AOAC method 2009.01 and to estimate their potential digestibility and assess their digestibility in vivo using glycaemic and insulinaemic responses as markers in mice and randomised clinical trial models. We found that fibre candidates to which α-(1,2) branching was added were resistant to digestion in the mouse model, depending on the amount of branching. These results show that in vivo models are needed to reliably assess the digestibility of α-glycosidic-linked oligomeric dietary fibre candidates, possibly due to absence of brush border α-glucosidase activity in the current in vitro assessment. α-(1,3)-linked and α-(1,6)-linked glucose oligomers were completely digested in humans and mice. In conclusion, it is possible to develop dietary soluble fibres by enzymatic synthesis. Adding α-(1,2) branching increases their resistance to digestion in vivo and can thus improve their suitability as potential fibre candidates. Clinical Trial Registry: ClinicalTrials.gov, NCT02701270.
authors.affiliation: a DuPont Nutrition and Health , Reigate , UK.
authors.forename: Oliver
authors.initials: O
authors.lastname: Hasselwander
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Robert
authors.initials: R
authors.lastname: DiCosimo
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: You
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Rothman
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Supaporn
authors.initials: S
authors.lastname: Suwannakham
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Zachary C
authors.initials: ZC
authors.lastname: Baer
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Roesch
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Kristin D
authors.initials: KD
authors.lastname: Ruebling-Jass
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Lai
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Hurteau
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Mario L
authors.initials: ML
authors.lastname: Marquez
authors.affiliation: b DuPont Industrial Biosciences , Wilmington , DE , USA.
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Kopatsis
authors.affiliation: c DuPont Nutrition and Health , Kantvik , Finland.
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Ouwehand
authors.affiliation: c DuPont Nutrition and Health , Kantvik , Finland.
authors.forename: Sofia D
authors.initials: SD
authors.lastname: Forssten
authors.affiliation: d DuPont Stine-Haskell , Newark , DE , USA.
authors.forename: Pushkor
authors.initials: P
authors.lastname: Mukerji
authors.affiliation: d DuPont Stine-Haskell , Newark , DE , USA.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Caverly Rae
authors.affiliation: d DuPont Stine-Haskell , Newark , DE , USA.
authors.forename: Yvonne P
authors.initials: YP
authors.lastname: Dragan
authors.affiliation: d DuPont Stine-Haskell , Newark , DE , USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Damewood
authors.affiliation: c DuPont Nutrition and Health , Kantvik , Finland.
authors.forename: Kirsti
authors.initials: K
authors.lastname: Tiihonen
authors.affiliation: c DuPont Nutrition and Health , Kantvik , Finland.
authors.forename: Alvin
authors.initials: A
authors.lastname: Ibarra
mesh_terms: D000328:Adult; D000818:Animals; D019540:Area Under Curve; D001419:Bacteria / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D004043:Dietary Fiber / Q000032:analysis* / Q000378:metabolism*; D004063:Digestion / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged
chemical_list: D001786:Blood Glucose; D004043:Dietary Fiber
keywords: Digestibility; glycaemia; insulinaemia; soluble fibre