title: Cullin-RING E3 Ubiquitin Ligases: Bridges to Destruction.
abstract: Ubiquitination is a highly conserved post-translational modification in eukaryotes, well known for targeting proteins for degradation by the 26S proteasome. Proteins destined for proteasomal degradation are selected by E3 ubiquitin ligases. Cullin-RING E3 ubiquitin ligases (CRLs) are the largest superfamily of E3 ubiquitin ligases, with over 400 members known in mammals. These modular complexes are tightly regulated in the cell. In this chapter, we highlight recent structural and biochemical advances shedding light on the assembly and architecture of cullin-RING ligases, their dynamic regulation by a variety of host factors, and their manipulation by viral pathogens and small molecules.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA.|Department of Biochemistry and Biophysics, University of California, San Francisco, CA, 94158, USA.
authors.forename: Henry C
authors.initials: HC
authors.lastname: Nguyen
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA. yong.xiong@yale.edu.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D000818:Animals; D044842:Cullin Proteins / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry* / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D044842:Cullin Proteins; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease
keywords: Cullin; Cullin-RING ligase; E3 ligase; NEDD8; Neddylation; Protein-protein interaction; Ubiquitin; Ubiquitination; Ubiquitylation; Viral hijacking