title: Analysis of 100-Year-Old Beer Originated from the Czech Republic.
abstract: Three bottles of different beers were found in 2015 during a reconstruction of the brewery of the Raven Trading s.r.o. company in Záhlinice, Czech Republic. Thanks to good storage conditions, it was possible to analyze their original characteristics. All three bottles contained most probably lager type beer. One beer had sulfuric and fecal off-flavors; it was bright with the original extract of 10.3° Plato. The second beer, with an original extract of 7.6° Plato, was dark and very acidic, resembling Lambic. DNA analysis proved the presence of Dekkera bruxellensis, which corresponded to its chemical profile (total acidity, FAN, ethyl acetate, total esters). The third beer contained traces of carbon dioxide bubbles, was light brown and slightly bitter, with an original extract 10.4° Plato. Because it obviously underwent a natural aging process, sweetness, honey, and fruity off-flavors were detected and transformation products of iso-α-acids were found.
authors.affiliation: Research Institute for Brewing and Malting, PLC , Lípová 15, CZ-120 44 Prague, Czech Republic.
authors.forename: Jana
authors.identifier: http://orcid.org/0000-0003-4938-3666
authors.initials: J
authors.lastname: Olšovská
authors.affiliation: Research Institute for Brewing and Malting, PLC , Lípová 15, CZ-120 44 Prague, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Matoulková
authors.affiliation: Research Institute for Brewing and Malting, PLC , Lípová 15, CZ-120 44 Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Dušek
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic , v.v.i., Vı́deňská 1038, CZ-142 20 Prague, Czech Republic.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Felsberg
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic , v.v.i., Vı́deňská 1038, CZ-142 20 Prague, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Jelínková
authors.affiliation: Research Institute for Brewing and Malting, PLC , Lípová 15, CZ-120 44 Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Čejka
authors.affiliation: Research Institute for Brewing and Malting, PLC , Lípová 15, CZ-120 44 Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Štěrba
mesh_terms: D000143:Acids / Q000032:analysis; D001515:Beer / Q000032:analysis* / Q000382:microbiology; D018153:Czech Republic; D055167:Dekkera / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D005421:Flavoring Agents / Q000032:analysis; D005511:Food Handling; D006801:Humans; D013997:Time Factors
chemical_list: D000143:Acids; D005227:Fatty Acids; D005421:Flavoring Agents
keywords: Dekkera bruxellensis; Saccharomyces; beer aging; carbonyl compounds; transformation of isohumulones