title: Circulating microRNAs Correlated with Bone Loss Induced by 45 Days of Bed Rest.
abstract: The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with bone loss induced by bed rest, and testify whether the selected miRNAs could reflect the bone mineral status of human after bed-rest. We analyzed plasma miRNA levels of 16 subjects after 45 days of -6° head-down tilt bed rest, which is a reliable model for the simulation of microgravity. We characterize the circulating miRNA profile in individuals after bed rest and identify circulating miRNAs which can best reflect the level of bone loss induced by bed rest. Expression profiling of circulating miRNA revealed significant downregulation of 37 miRNAs and upregulation of 2 miRNAs, while only 11 of the downregulated miRNAs were further validated in a larger volunteer cohort using qPCR. We found that 10 of these 11 miRNAs (miR-103, 130a, 1234, 1290, 151-5p, 151-3p, 199a-3p, 20a, 363, and 451a) had ROC curve that distinguished the status after bed rest. Importantly, significant positive correlations were identified between bone loss parameters and several miRNAs, eventually miR-1234 showed clinical significance in detecting the bone loss of individuals after 45 days of bed rest.
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Shukuan
authors.initials: S
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhong
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training CenterBeijing, China; Laboratory of Longitudinal Integration of Individual Life Data, Space Institute of Southern ChinaShenzhen, China.
authors.forename: Fengji
authors.initials: F
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training CenterBeijing, China; Key Laboratory of Molecular and Cellular Biology of Ministry of Education, College of Life Science, Hebei Normal UniversityShijiazhuang, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Dingsheng
authors.initials: D
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training CenterBeijing, China; Key Laboratory of Molecular and Cellular Biology of Ministry of Education, College of Life Science, Hebei Normal UniversityShijiazhuang, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Song
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training CenterBeijing, China; Laboratory of Longitudinal Integration of Individual Life Data, Space Institute of Southern ChinaShenzhen, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Key Laboratory of Human Factors Engineering, China Astronaut Research and Training Center Beijing, China.
authors.forename: Shanguang
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training CenterBeijing, China; Laboratory of Longitudinal Integration of Individual Life Data, Space Institute of Southern ChinaShenzhen, China.
authors.forename: Jianghui
authors.initials: J
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
authors.forename: Yingxian
authors.initials: Y
authors.lastname: Li
keywords: bed rest; bone loss; circulating miRNA; miR-1234; simulated microgravity