title: Enhanced photostability, radical scavenging and antitumor activity of indole-3-carbinol-loaded rose hip oil nanocapsules.
abstract: This study aimed to develop poly(ε-caprolactone) nanocapsules loaded with indole-3-cabinol (I3C) using rose hip oil (RHO) or medium chain triglycerides (MCT) as oil core. In vitro radical scavenging activity (DPPH method), hemolysis, and antitumor effects on breast (MCF-7) and glioma (C6) cells were conducted. Preformulation evaluations revealed that RHO is suitable to prepare the nanocapsules considering the log P determination and dissolution/swelling experiments of polymer films. The nanocapsules were prepared and presented adequate physicochemical characteristics as mean size around 250nm, polydispersity index values <0.2, zeta potential negative values and I3C encapsulation efficiency around 42%, without any influence of the oil core (RHO or MCT) on these parameters. However, the photodegradation study demonstrated that RHO nanocapsules showed less degree of I3C degradation in comparison to MCT nanocapsules. The in vitro release profile showed that both nanocapsule suspensions demonstrated an initial burst effect followed by a prolonged I3C release. In addition, the formulations were considered hemocompatibles at 10μg/mL and showed an enhanced radical scavenging activity in comparison to free I3C. Moreover, nanocapsules prepared with RHO increased about two times the antitumor effect of I3C on MCF-7 and C6 cells without significant reduction of astrocyte cell viability. In conclusion, nanocapsule formulations developed in this study might be considered promising for cancer treatment.
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Mailine
authors.initials: M
authors.lastname: Gehrcke
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Laura Minussi
authors.initials: LM
authors.lastname: Giuliani
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Luana Mota
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Allanna Valentini
authors.initials: AV
authors.lastname: Barbieri
authors.affiliation: Programa de Pós-graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Marcel Henrique Marcondes
authors.initials: MHM
authors.lastname: Sari
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Elita Ferreira
authors.initials: EF
authors.lastname: da Silveira
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Juliana Hofstatter
authors.initials: JH
authors.lastname: Azambuja
authors.affiliation: Programa de Pós-graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Cristina Wayne
authors.initials: CW
authors.lastname: Nogueira
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, RS, Brazil.; Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Elizandra
authors.initials: E
authors.lastname: Braganhol
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: leticiacruz@smail.ufsm.br.
authors.forename: Letícia
authors.initials: L
authors.lastname: Cruz
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D061986:MCF-7 Cells; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry* / Q000633:toxicity; D009822:Oils, Volatile / Q000737:chemistry*; D010782:Photolysis / Q000187:drug effects; D027826:Rosa / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility; D014466:Ultraviolet Rays
chemical_list: D000970:Antineoplastic Agents; D016166:Free Radical Scavengers; D007211:Indoles; D053769:Nanocapsules; D009822:Oils, Volatile; C016517:indole-3-carbinol
keywords: Antitumor activity; Indole-3-carbinol; Nanoparticles; Radical scavenging activity; Rose hip oil