title: Response of microbial community structure to pre-acclimation strategies in microbial fuel cells for domestic wastewater treatment.
abstract: Microbial community structures and performance of air-cathode microbial fuel cells (MFCs) inoculated with activated sludge from domestic wastewater were investigated to evaluate the effects of three substrate pre-acclimation strategies: 1, serial pre-acclimation with acetate and glucose before supplying domestic wastewater; 2, one step pre-acclimation with acetate before supplying domestic wastewater; and 3, direct supply of domestic wastewater without any pre-acclimation. Strategy 1 showed much higher current generation (1.4mA) and Coulombic efficiency (33.5%) than strategies 2 (0.7mA and 9.4%) and 3 (0.9mA and 10.3%). Pyrosequencing showed that microbial communities were significantly affected by pre-acclimation strategy. Although Proteobacteria was the dominant phylum with all strategies, Actinobacteria was abundant when MFCs were pre-acclimated with glucose after acetate. Not only anode-respiring bacteria (ARB) in the genus Geobacter but also non-ARB belonging to the family Anaerolinaceae seemed to play important roles in air-cathode MFCs to produce electricity from domestic wastewater.
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Younghyun
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Hyunwoo
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jaechul
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Environmental Science and Engineering, Kyung Hee University, 1 Seocheon-dong, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Booki
authors.initials: B
authors.lastname: Min
authors.affiliation: The MFC Research and Business Development (R&BD) Center, K-water Institute, Jeonmin-Dong, Yuseong-Gu, Daejeon 305-730, Republic of Korea.
authors.forename: Hong Suck
authors.initials: HS
authors.lastname: Kim
authors.affiliation: The MFC Research and Business Development (R&BD) Center, K-water Institute, Jeonmin-Dong, Yuseong-Gu, Daejeon 305-730, Republic of Korea.
authors.forename: Byung Goon
authors.initials: BG
authors.lastname: Kim
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: leeth55@pusan.ac.kr.
authors.forename: Taeho
authors.initials: T
authors.lastname: Lee
mesh_terms: D000064:Acclimatization; D001419:Bacteria; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004560:Electricity; D004566:Electrodes; D012722:Sewage / Q000382:microbiology; D062065:Wastewater / Q000382:microbiology*
chemical_list: D012722:Sewage; D062065:Wastewater
keywords: Air-cathode microbial fuel cell; Anode-respiring bacteria; Domestic wastewater; Microbial community structure; Pyrosequencing