title: Influence of dynamic flow environment on nanoparticle-protein corona: From protein patterns to uptake in cancer cells.
abstract: The fast growing use of nanoparticles (NPs) in biotechnology and biomedicine raises concerns about human health and the environment. When introduced in physiological milieus, NPs adsorb biomolecules (especially proteins) forming the so-called protein corona (PC). As it is the PC that mostly interacts with biological systems, it represents a major element of the NPs' biological identity with impact on nanotoxicology, nanosafety and targeted delivery of nanomedicines. To date, NP-protein interactions have been largely investigated in vitro, but this condition is far from mimicking the dynamic nature of physiological environments. Here we investigate the effect of shear stress on PC by exposing lipid NPs with different surface chemistry (either unmodified and PEGylated) to circulating fetal bovine serum (FBS). PC formed upon in vitro incubation was used as a reference. We demonstrate that PC is significantly influenced by exposure to dynamic flow and that changes in PC composition are dependent on both exposure time and NP's surface chemistry. Notably, alterations induced by dynamic flow affected cellular uptake of lipid NPs in both human cervical cancer (HeLa) and human breast adenocarcinoma (MCF7) cell lines.
authors.affiliation: Department of Molecular Medicine, "Sapienza" University of Rome, Viale Regina Elena 291, 00161, Rome, Italy; Istituti Fisioterapici Ospitalieri, Istituto Regina Elena,Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Palchetti
authors.affiliation: Department of Molecular Medicine, "Sapienza" University of Rome, Viale Regina Elena 291, 00161, Rome, Italy; Istituti Fisioterapici Ospitalieri, Istituto Regina Elena,Via Elio Chianesi 53, 00144 Rome, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Pozzi
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
authors.forename: Anna Laura
authors.initials: AL
authors.lastname: Capriotti
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
authors.forename: Giorgia La
authors.initials: G
authors.lastname: Barbera
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
authors.forename: Riccardo Zenezini
authors.initials: RZ
authors.lastname: Chiozzi
authors.affiliation: Department of Bioscience and Biotechnology, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Digiacomo
authors.affiliation: Istituto Italiano di Tecnologia, Center for Life Nano Science@Sapienza, Viale Regina Elena 291, 00161, Rome, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Peruzzi
authors.affiliation: Department of Molecular Medicine, "Sapienza" University of Rome, Viale Regina Elena 291, 00161, Rome, Italy. Electronic address: giulio.caracciolo@uniroma1.it.
authors.forename: Giulio
authors.initials: G
authors.lastname: Caracciolo
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
mesh_terms: D000818:Animals; D002417:Cattle; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism*; D000066970:Protein Corona / Q000737:chemistry* / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D000066970:Protein Corona
keywords: Cancer cells; Dynamic flow environment; Nanoparticles; Physiological environments; Protein corona