title: Formation of low molecular weight oligomers from chitin and chitosan stimulated by plasma-assisted processes.
abstract: The controlled degradation of solid powders of chitin and chitosan stimulated by electron-beam plasma (EBP) was experimentally studied. Crab shell chitin and chitosan were used as original substances. The non-equilibrium low temperature EBP was generated by injecting an electron beam into a gaseous medium. Chitooligosaccharides with Mw=800-2000Da and polydispersion 1.5-2.5 were formed due to the EBP-treatment of chitin and chitosan. The β-1,4 glycosidic bounds in original polymers degrade under the action of active oxygen species produced in the EBP. Low molecular weight products of chitosan inhibited the growth of various yeast-like and filamentous fungi at minimum inhibitory concentration 500mcg/ml. By optimizing the treatment conditions and using special techniques of reaction volume formation the 95% yield of chitooligosaccharides was obtained after 2min whereas the conventional chemical hydrolysis usually takes several days. The EBP-stimulated hydrolysis is promising for effective polysaccharides degradation and can be competitive with traditional technologies.
authors.affiliation: Department of General Chemistry, Moscow Institute of Physics and Technology, Institutsky per., 9, Dolgoprudny, Moscow Region, 141 700, Russia. Electronic address: tmvasilieva@gmail.com.
authors.forename: T
authors.initials: T
authors.lastname: Vasilieva
authors.affiliation: Department of General Chemistry, Moscow Institute of Physics and Technology, Institutsky per., 9, Dolgoprudny, Moscow Region, 141 700, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Sigarev
authors.affiliation: Core Facility Center "Arktika", Northern (Arctic) Federal University, Severnaya Dvina Emb., 17, Arkhangelsk, Russia. Electronic address: kosyakov@mail.ru.
authors.forename: D
authors.initials: D
authors.lastname: Kosyakov
authors.affiliation: Core Facility Center "Arktika", Northern (Arctic) Federal University, Severnaya Dvina Emb., 17, Arkhangelsk, Russia. Electronic address: uluanovskii@mail.ru.
authors.forename: N
authors.initials: N
authors.lastname: Ul'yanovskii
authors.affiliation: Core Facility Center "Arktika", Northern (Arctic) Federal University, Severnaya Dvina Emb., 17, Arkhangelsk, Russia. Electronic address: easorokina1992@mail.ru.
authors.forename: E
authors.initials: E
authors.lastname: Anikeenko
authors.affiliation: Core Facility Center "Arktika", Northern (Arctic) Federal University, Severnaya Dvina Emb., 17, Arkhangelsk, Russia. Electronic address: easorokina1992@mail.ru.
authors.forename: D
authors.initials: D
authors.lastname: Chuhchin
authors.affiliation: Core Facility Center "Arktika", Northern (Arctic) Federal University, Severnaya Dvina Emb., 17, Arkhangelsk, Russia. Electronic address: lokoal13@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Ladesov
authors.affiliation: Aerospace Research Department, Moscow Institute of Physics and Technology, Institutsky per., 9, Dolgoprudny, Moscow Region, 141 700, Russia. Electronic address: greatofaungmyathein52@gmail.com.
authors.forename: Aung Myat
authors.initials: AM
authors.lastname: Hein
authors.affiliation: Aerospace Research Department, Moscow Institute of Physics and Technology, Institutsky per., 9, Dolgoprudny, Moscow Region, 141 700, Russia. Electronic address: vladimir.myasnikov@phystech.edu.
authors.forename: V
authors.initials: V
authors.lastname: Miasnikov
mesh_terms: D000818:Animals; D003386:Brachyura; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D006868:Hydrolysis; D008970:Molecular Weight; D009844:Oligosaccharides
chemical_list: D009844:Oligosaccharides; D002686:Chitin; D048271:Chitosan
keywords: Bioactive oligosaccharides; Chitin; Chitosan; Electron-beam plasma; Plasma-stimulated hydrolysis