title: Management of E. coli sister chromatid cohesion in response to genotoxic stress.
abstract: Aberrant DNA replication is a major source of the mutations and chromosomal rearrangements associated with pathological disorders. In bacteria, several different DNA lesions are repaired by homologous recombination, a process that involves sister chromatid pairing. Previous work in Escherichia coli has demonstrated that sister chromatid interactions (SCIs) mediated by topological links termed precatenanes, are controlled by topoisomerase IV. In the present work, we demonstrate that during the repair of mitomycin C-induced lesions, topological links are rapidly substituted by an SOS-induced sister chromatid cohesion process involving the RecN protein. The loss of SCIs and viability defects observed in the absence of RecN were compensated by alterations in topoisomerase IV, suggesting that the main role of RecN during DNA repair is to promote contacts between sister chromatids. RecN also modulates whole chromosome organization and RecA dynamics suggesting that SCIs significantly contribute to the repair of DNA double-strand breaks (DSBs).
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR CNRS-7241, INSERM U1050, PSL Research University, 11 place Marcelin Berthelot, 75005 Paris, France.|Université Paris-Saclay, 91400 Orsay, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Vickridge
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR CNRS-7241, INSERM U1050, PSL Research University, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Charlene
authors.initials: C
authors.lastname: Planchenault
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR CNRS-7241, INSERM U1050, PSL Research University, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Cockram
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR CNRS-7241, INSERM U1050, PSL Research University, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Isabel Garcia
authors.initials: IG
authors.lastname: Junceda
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR CNRS-7241, INSERM U1050, PSL Research University, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Espéli
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology; D002842:Chromatids / Q000378:metabolism*; D020090:Chromosome Segregation / Q000502:physiology; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology*; D004261:DNA Replication / Q000502:physiology; D004262:DNA Restriction Enzymes / Q000502:physiology; D027101:DNA Topoisomerase IV / Q000502:physiology; D004269:DNA, Bacterial / Q000187:drug effects / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D016685:Mitomycin / Q000494:pharmacology; D011938:Rec A Recombinases / Q000502:physiology; D013014:SOS Response, Genetics / Q000187:drug effects / Q000502:physiology; D012854:Sister Chromatid Exchange / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D016685:Mitomycin; D011938:Rec A Recombinases; D004262:DNA Restriction Enzymes; C047879:RecN protein, Bacteria; D027101:DNA Topoisomerase IV