title: NIR-emitting and photo-thermal active nanogold as mitochondria-specific probes.
abstract: We report a bioinspired multifunctional albumin derived polypeptide coating comprising grafted poly(ethylene oxide) chains, multiple copies of the HIV TAT derived peptide enabling cellular uptake as well as mitochondria targeting triphenyl-phosphonium (TPP) groups. Exploring these polypeptide copolymers for passivating gold nanoparticles (Au NPs) yielded (i) NIR-emitting markers in confocal microscopy and (ii) photo-thermal active probes in optical coherence microscopy. We demonstrate the great potential of such multifunctional protein-derived biopolymer coatings for efficiently directing Au NP into cells and to subcellular targets to ultimately probe important cellular processes such as mitochondria dynamics and vitality inside living cells.
authors.affiliation: Institute of Organic Chemistry III, Ulm University, Albert-Einstein-Allee 11, D-89081, Ulm, Germany. Tanja.Weil@uni-ulm.de.
authors.forename: Sabyasachi
authors.initials: S
authors.lastname: Chakrabortty
authors.forename: Miguel
authors.initials: M
authors.lastname: Sison
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Wu
authors.forename: Anita
authors.initials: A
authors.lastname: Ladenburger
authors.forename: Goutam
authors.initials: G
authors.lastname: Pramanik
authors.forename: Johannes
authors.initials: J
authors.lastname: Biskupek
authors.forename: Jerome
authors.initials: J
authors.lastname: Extermann
authors.forename: Ute
authors.initials: U
authors.lastname: Kaiser
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser
authors.forename: Tanja
authors.initials: T
authors.lastname: Weil
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods; D008928:Mitochondria / Q000648:ultrastructure*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D013696:Temperature; D013730:Terphenyl Compounds / Q000737:chemistry; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009943:Organophosphorus Compounds; D010455:Peptides; D012709:Serum Albumin; D013730:Terphenyl Compounds; D054322:tat Gene Products, Human Immunodeficiency Virus; D011092:Polyethylene Glycols; D006046:Gold