title: Intravenous Administration of Adipose-Derived Stem Cell Protein Extracts Improves Neurological Deficits in a Rat Model of Stroke.
abstract: Treatment of adipose-derived stem cell (ADSC) substantially improves the neurological deficits during stroke by reducing neuronal injury, limiting proinflammatory immune responses, and promoting neuronal repair, which makes ADSC-based therapy an attractive approach for treating stroke. However, the potential risk of tumorigenicity and low survival rate of the implanted cells limit the clinical use of ADSC. Cell-free extracts from ADSC (ADSC-E) may be a feasible approach that could overcome these limitations. Here, we aim to explore the potential usage of ADSC-E in treating rat transient middle cerebral artery occlusion (tMCAO). We demonstrated that intravenous (IV) injection of ADSC-E remarkably reduces the ischemic lesion and number of apoptotic neurons as compared to other control groups. Although ADSC and ADSC-E treatment results in a similar degree of a long-term clinical beneficial outcome, the dynamics between two ADSC-based therapies are different. While the injection of ADSC leads to a relatively mild but prolonged therapeutic effect, the administration of ADSC-E results in a fast and pronounced clinical improvement which was associated with a unique change in the molecular signature suggesting that potential mechanisms underlying different therapeutic approach may be different. Together these data provide translational evidence for using protein extracts from ADSC for treating stroke.
authors.affiliation: Department of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Neuroinflammation Unit, Montreal Neurological Institute, McGill University, Montreal, QC, Canada H3A 2B4.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors.forename: Changcong
authors.initials: C
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors.forename: Xize
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors.forename: Wanping
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Pei
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors.forename: Linlu
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150001, China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Neurosurgery, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150001, China.
authors.forename: Huakun
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Immunology, Harbin Medical University, Harbin, Heilongjiang 150086, China.
authors.forename: Hongwei
authors.identifier: 0000-0001-5763-3894
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, The First Affiliate Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
authors.forename: Lixian
authors.identifier: 0000-0003-4353-2229
authors.initials: L
authors.lastname: Li