title: Characterization and in vitro evaluation of biphasic α-tricalcium phosphate/β-tricalcium phosphate cement.
abstract: Biphasic calcium phosphate consisting of hydroxyapatite (HA) and β-tricalcium phosphate(β-TCP) is an excellent bone substitute with controllable bioresorbability. Fabrication of biphasic calcium phosphate with self-setting ability is expected to enhance its potential application as bone substitute. In this study, mixtures of α-TCP and β-TCP with various compositions were prepared through α-β phase transition of α-TCP powder at 1000°C for various periods. These powders were mixed with 0.25M Na2HPO4at a P/L ratio of 2, and then hardened at 37°C at 100% RH for up to 24h. Material properties of biphasic HA/β-TCP cement with different α-TCP/β-TCP composition were characterized. These cements were also evaluated with respect to cell response in vitro using MC3T3-E1 cell lines. In conclusion, mechanical and biological properties of HA/β-TCP cement could be controlled by changing the heat treatment time of α-TCP powder at 1000°C. In vitro results indicated that cell proliferation and ALP activity increased with increase β-TCP content.
authors.affiliation: Section of Bioengineering, Department of Dental Engineering, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Arahira
authors.affiliation: Section of Bioengineering, Department of Dental Engineering, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan. Electronic address: maruta@college.fdcnet.ac.jp.
authors.forename: Michito
authors.initials: M
authors.lastname: Maruta
authors.affiliation: Section of Bioengineering, Department of Dental Engineering, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Matsuya
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006358:Hot Temperature; D051379:Mice; D008855:Microscopy, Electron, Scanning; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485817:beta-tricalcium phosphate
keywords: Biomaterial; Biphasic cement; Hydroxyapatite; β-TCP