title: [Effects of dietary monosodium-glutamate on gene expression].
abstract: BACKGROUND
  Nowadays, the food industry more often uses different type of additives during the food production.
  
  
  OBJECTIVE
  Our aim was to examine the monosodium-glutamate's effect (in animal experiment) on DNA-methyltransferases in gene expression patterns of mRNA levels.
  
  
  METHODS
  In the investigation we used 24 (n=24) CD1 type female mice. The animals were fed with different equivalent human doses of the tested substance. After autopsy, mRNA was isolated from different tissues (lung, liver, kidney, spleen). DNMT1, DNMT3A and DNMT3B levels were determined by Quantitative Real-Time PCR.
  
  
  RESULTS
  DNMT1 significantly suppressed the gene expression in all the three treated groups (p<0.05). The DNMT3A expression patterns showed significant decreasing tendency in the 1. and 2. treated groups of the lung tissue (p<0,05) and 1, 2, 3. groups of liver and kidney tissues (p<0,05).
  
  
  CONCLUSIONS
  Our results shows that the monosodium glutamate, suppressed the DNMT1 and DNMT3A gene expression - on mRNA levels of several organs - in mice. It can be a similar chemopreventive effect to epigallo-catechin-gallate's, curcumin's, genistein's, likopine's and rezveratrol's effects. In this case it can be possible that the MSG has anticarcinogenic effects. Orv. Hetil., 2017, 158(10), 380-385.
authors.affiliation: Táplálkozástudományi és Dietetikai Intézet, Pécsi Tudományegyetem, Egészségtudományi Kar Pécs.
authors.forename: Júlia Vanda
authors.initials: JV
authors.lastname: Jurasek
authors.affiliation: Táplálkozástudományi és Dietetikai Intézet, Pécsi Tudományegyetem, Egészségtudományi Kar Pécs.
authors.forename: László Bence
authors.initials: LB
authors.lastname: Raposa
authors.affiliation: Táplálkozástudományi és Dietetikai Intézet, Pécsi Tudományegyetem, Egészségtudományi Kar Pécs.
authors.forename: Andrea
authors.initials: A
authors.lastname: Gubicskóné Kisbenedek
authors.affiliation: Fizioterápiás és Sporttudományi Intézet, Pécsi Tudományegyetem, Egészségtudományi Kar Pécs.
authors.forename: Veronika
authors.initials: V
authors.lastname: Varga
authors.affiliation: Táplálkozástudományi és Dietetikai Intézet, Pécsi Tudományegyetem, Egészségtudományi Kar Pécs.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.affiliation: Orvosi Népegészségtani Intézet, Pécsi Tudományegyetem, Általános Orvostudományi Kar Pécs.
authors.forename: Tímea
authors.initials: T
authors.lastname: Varjas
mesh_terms: D000818:Animals; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000187:drug effects*; D000090205:DNA Methyltransferase 3A; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012970:Sodium Glutamate / Q000494:pharmacology*; D000097584:DNA Methyltransferase 3B
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D012333:RNA, Messenger; D012970:Sodium Glutamate; D000097584:DNA Methyltransferase 3B; C000717967:DNMT3A protein, human; C000715535:Dnmt3a protein, mouse; C000619380:DNMT1 protein, human; C000619381:Dnmt1 protein, mouse
keywords: DNA-methyltransferase 1; DNA-methyltransferase 3a; DNA-methyltransferase 3b; DNMT1; DNMT3a; DNMT3b; food additive; gene expression; génexpresszió; monosodium-glutamate; nátrium-glutamát; élelmiszer-adalékanyag