title: Medium pH in submerged cultivation modulates differences in the intracellular protein profile of Fusarium oxysporum.
abstract: Fusarium oxysporum is a filamentous fungus that damages a wide range of plants and thus causes severe crop losses. In fungal pathogens, the genes and proteins involved in virulence are known to be controlled by environmental pH. Here, we report the influence of culture-medium pH (5, 6, 7, and 8) on the production of degradative enzymes involved in the pathogenesis of F. oxysporum URM 7401 and on the 2D-electrophoresis profile of intracellular proteins in this fungus. F. oxysporum URM 7401 was grown in acidic, neutral, and alkaline culture media in a submerged bioprocess. After 96 hr, the crude extract was processed to enzyme activity assays, while the intracellular proteins were obtained from mycelium and analyzed using 2D electrophoresis and mass spectrometry. We note that the diversity of secreted enzymes was changed quantitatively in different culture-medium pH. Also, the highest accumulated biomass and the intracellular protein profile of F. oxysporum URM 7401 indicate an increase in metabolism in neutral-alkaline conditions. The differential profiles of secreted enzymes and intracellular proteins under the evaluated conditions indicate that the global protein content in F. oxysporum URM 7401 is modulated by extracellular pH.
authors.affiliation: a School of Pharmaceutical Sciences of Ribeirão Preto, Department of Pharmaceutical Sciences , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Nathália Gonsales
authors.initials: NG
authors.lastname: da Rosa-Garzon
authors.affiliation: b School of Medicine of Ribeirão Preto, Department of Molecular and Cellular Biology and Pathogenic Bioagents , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Hélen Julie
authors.initials: HJ
authors.lastname: Laure
authors.affiliation: c Department of Mycology , Federal University of Pernambuco , Recife , PE , Brazil.
authors.forename: Cristina Maria de
authors.initials: CM
authors.lastname: Souza-Motta
authors.affiliation: b School of Medicine of Ribeirão Preto, Department of Molecular and Cellular Biology and Pathogenic Bioagents , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: José César
authors.initials: JC
authors.lastname: Rosa
authors.affiliation: a School of Pharmaceutical Sciences of Ribeirão Preto, Department of Pharmaceutical Sciences , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Hamilton
authors.initials: H
authors.lastname: Cabral
mesh_terms: D003470:Culture Media / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D040901:Proteomics
chemical_list: D003470:Culture Media; D005656:Fungal Proteins
keywords: Biotechnology; fungi; hydrolases; proteomics