title: Reliability of automated synovial fluid cell counting with Mindray BC-6800 body fluid mode.
abstract: BACKGROUND
  The enumeration and differentiation of nuclear elements in synovial fluid is a cornerstone for diagnosis and follow-up of many orthopedic and rheumatologic diseases. In this study, we evaluated the analytical performance of Mindray BC-6800 BF mode (BC-6800-BF) for synovial fluid analysis.
  
  
  METHODS
  Overall, 78 synovial fluids were collected and analyzed with both BC-6800-BF and light microscopy. The study also entailed the assessment of limit of blank (LoB), limit of detection (LoD), limit of quantification (LoQ), carryover and linearity.
  
  
  RESULTS
  The LoB for the parameters total cells and white blood cells was 6 × 106cells/L, and the LoD and LoQ were instead 15 and 16 × 106cells/L, respectively. Linearity was excellent and carryover was negligible. The agreement between BC-6800-BF and light microscopy was satisfactory for all samples pretreated with hyaluronidase, displaying a bias between -5.9% and 8.2%.
  
  
  CONCLUSIONS
  The use of BC-6800-BF for synovial fluid analysis enables rapid and accurate assessment, especially for total cell and polymorphonuclear counts. The use of BC-6800-BF may therefore allow the replacement of optical analysis, especially in samples pretreated with hyaluronidase, thus allowing its routine use for the screening of synovial specimens.
authors.affiliation: Clinical Chemistry Laboratory, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Buoro
authors.affiliation: Clinical Chemistry Laboratory, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Seghezzi
authors.affiliation: Clinical Chemistry Laboratory, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Manenti
authors.affiliation: Clinical Chemistry Laboratory, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Mecca
authors.affiliation: Pathology, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Candiago
authors.affiliation: Immunohaematology and Transfusion Medicine Unit, SS Trinità Hospital, Borgomanero, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Vidali
authors.affiliation: Synlab, Monza, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Ottomano
authors.affiliation: Orthopedics and Traumatology Unit, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: C C
authors.initials: CC
authors.lastname: Castelli
authors.affiliation: Orthopedics and Traumatology Unit, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Ferrari
authors.affiliation: Orthopedics and Traumatology Unit, Hospital Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Zappalà
authors.affiliation: Section of Clinical Biochemistry, University of Verona, Verona, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Lippi
mesh_terms: D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000737:chemistry; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012216:Rheumatic Diseases / Q000378:metabolism* / Q000473:pathology; D013582:Synovial Fluid / Q000378:metabolism*
chemical_list: D006821:Hyaluronoglucosaminidase
keywords: BC-6800; Mindray; Synovial fluid; body fluid; light microscopy