title: Reaction mechanism of blood coagulation factors in shear flow field.
abstract: Thrombus formation in blood pumps is a major problem. It has been reported that the shear rate is closely related to thrombus formation in blood pumps; however, the mechanism of blood coagulation in a shear flow field is not yet fully understood. The purpose of this study is to evaluate the effect of shear loading on the reaction of blood coagulation factors quantitatively. Human blood and porcine blood were used as test blood and sheared at 2,880 s-1 for 3 h using a rheometer. After shear loading to the test blood, the activated partial thromboplastin time (APTT) and prothrombin time (PT) of sample plasma were measured using an automated coagulation analyzer. The APTT and PT of mixed plasma containing human blood coagulation factor-deficient plasma and sample plasma were also measured. The APTT and PT of mixed plasma reflected the reaction of the particular blood coagulation factor. Results show that shear loading prolonged the APTT and PT of human sample plasma. Accordingly, the APTT and PT of mixed plasma containing human blood coagulation factor V-deficient plasma and human sample plasma were prolonged after shear loading. Thus, the reaction of human blood coagulation factors was inhibited by specific inhibition of the reaction of human blood coagulation factor V in the shear flow field. The reaction time of porcine blood coagulation factors after shear loading differed from that of human blood coagulation factors. The result suggest the evaluation difficulty of animal blood coagulation reaction, that is, porcine blood coagulation reaction did not fully proceed with clinical reagents due to species specificity.
authors.forename: Koki
authors.initials: K
authors.lastname: Kawakami
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakota
authors.forename: Ryo
authors.initials: R
authors.lastname: Kosaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishida
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Osamu
authors.initials: O
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000378:metabolism*; D005165:Factor V / Q000378:metabolism; D006801:Humans; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D012212:Rheology; D033081:Shear Strength; D013552:Swine
chemical_list: D001779:Blood Coagulation Factors; D005165:Factor V