title: High levels of serum platelet-derived growth factor-AA and human epidermal growth factor receptor-2 are predictors of colorectal cancer liver metastasis.
abstract: OBJECTIVE
  To develop predictive markers in blood for colorectal cancer liver metastasis.
  
  
  METHODS
  Twenty colorectal cancer patients were selected and divided into two groups. Group A consisted of 10 patients whose pathological TNM stage was IIIC (T3-4N2M0), while another 10 patients with synchronous liver metastasis (TNM stage IV) were recruited for group B. During the surgical procedure, a 10-mL drainage vein (DV) blood sample was obtained from the DV of the tumor-bearing segment prior to the ligation of the DV. At the same time, a 10-mL peripheral vein (PV) blood sample was collectedviaperipheral venipuncture. The serum levels of 24 molecules that are potentially involved in the mechanism of liver metastasis in both DV blood and PV blood were analyzed by using high-throughput enzyme-linked immunosorbent assay technology.
  
  
  RESULTS
  Univariate analysis revealed that platelet-derived growth factor AA (PDGFAA) in DV blood (dPDGFAA) (P= 0.001), PDGFAA in PV blood (pPDGFAA) (P= 0.007), and human epidermal growth factor receptor-2 in PV blood (pHER2) (P= 0.001), pMMP7 (P= 0.028), pRANTES (P= 0.013), and pEGF (P= 0.007) were significantly correlated with synchronous liver metastasis. Multivariate analysis identified dPDGFAA (HR = 1.001,P= 0.033) and pHER2 (HR = 1.003,P= 0.019) as independent predictive factors for synchronous liver metastasis. Besides, high peripheral HER2 level may also be a risk factor for metachronous liver metastasis, although the difference did not reach statistical significance (P= 0.06). Significant correlations were found between paired DV and PV blood levels for PDGFAA (r= 0.794,P< 0.001), but not for HER2 (r= 0.189,P= 0.424).
  
  
  CONCLUSIONS
  PDGFAA in tumor drainage and HER2 in PV blood may be useful predictive factors for synchronous liver metastasis of colorectal cancer.
authors.affiliation: Hong-Da Pan, Gang Xiao, Department of Gastrointestinal Surgery, Beijing Hospital, National Center of Gerontology, Beijing 100730, China.
authors.forename: Hong-Da
authors.initials: HD
authors.lastname: Pan
authors.affiliation: Hong-Da Pan, Gang Xiao, Department of Gastrointestinal Surgery, Beijing Hospital, National Center of Gerontology, Beijing 100730, China.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Peng
authors.affiliation: Hong-Da Pan, Gang Xiao, Department of Gastrointestinal Surgery, Beijing Hospital, National Center of Gerontology, Beijing 100730, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xiao
authors.affiliation: Hong-Da Pan, Gang Xiao, Department of Gastrointestinal Surgery, Beijing Hospital, National Center of Gerontology, Beijing 100730, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Gu
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011237:Predictive Value of Tests; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood*; D012189:Retrospective Studies
chemical_list: D010982:Platelet-Derived Growth Factor; D018719:Erb-b2 Receptor Tyrosine Kinases; C075278:platelet-derived growth factor A; C508053:ERBB2 protein, human
keywords: Colorectal cancer; Human epidermal growth factor receptor-2; Liver metastasis; Platelet-derived growth factor AA