title: A Focused Multiple Reaction Monitoring (MRM) Quantitative Method for Bioactive Grapevine Stilbenes by Ultra-High-Performance Liquid Chromatography Coupled to Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ).
abstract: Grapevine stilbenes are a family of polyphenols which derive fromtrans-resveratrol having antifungal and antimicrobial properties, thus being considered as phytoalexins. In addition to their diverse bioactive properties in animal models, they highlight a strong potential in human health maintenance and promotion. Due to this relevance, highly-specific qualitative and quantitative methods of analysis are necessary to accurately analyze stilbenes in different matrices derived from grapevine. Here, we developed a rapid, sensitive, and specific analysis method using ultra-high-performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ) in MRM mode to detect and quantify five grapevine stilbenes,trans-resveratrol,trans-piceid,trans-piceatannol,trans-pterostilbene, andtrans-ε-viniferin, whose interest in relation to human health is continuously growing. The method was optimized to minimize in-source fragmentation of piceid and to avoid co-elution ofcis-piceid andtrans-resveratrol, as both are detected with resveratrol transitions. The applicability of the developed method of stilbene analysis was tested successfully in different complex matrices including cellular extracts ofVitis viniferacell cultures, reaction media of biotransformation assays, and red wine.
authors.affiliation: Departamento Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, 03690 Alicante, Spain. ehurtadog7@gmail.com.
authors.forename: Elías
authors.initials: E
authors.lastname: Hurtado-Gaitán
authors.affiliation: Departamento Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, 03690 Alicante, Spain. susana.selles@ua.es.|Genomics and Proteomics Unit, SSTTI Universidad de Alicante, 03690 Alicante, Spain. susana.selles@ua.es.
authors.forename: Susana
authors.initials: S
authors.lastname: Sellés-Marchart
authors.affiliation: Departamento Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, 03690 Alicante, Spain. asun.martinez@ua.es.
authors.forename: Ascensión
authors.initials: A
authors.lastname: Martínez-Márquez
authors.affiliation: Departamento Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, 03690 Alicante, Spain. antonio.samper@ua.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Samper-Herrero
authors.affiliation: Departamento Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, 03690 Alicante, Spain. roque.bru@ua.es.|Instituto de Investigación Sanitaria y Biomédica de Alicante ISABIAL-Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana FISABIO, 03010 Alicante, Spain. roque.bru@ua.es.
authors.forename: Roque
authors.initials: R
authors.lastname: Bru-Martínez
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D064209:Phytochemicals; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D001688:Biological Products; D064209:Phytochemicals; D013267:Stilbenes; D000077185:Resveratrol
keywords: MRM; UHPLC; bioconversion; cell culture; epsilon-viniferin; piceatannol; piceid; pterostilbene; resveratrol; wine