title: The pre-mir-499 Variant rs3746444 May Contribute to Coronary Artery Disease Susceptibility: a Case-Control and Meta-Analysis Study.
abstract: BACKGROUND
  Recent reports have suggested that single-nucleotide polymorphisms (SNPs) in microRNA genes may contribute to individual susceptibility to coronary artery disease (CAD). The purpose of this study was to evaluate the association between rs3746444 in pre-miR-499 with CAD.
  
  
  METHODS
  We performed a case-control study including 288 CAD patients and 150 control subjects. DNA was extracted from blood samples and genotyped through the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Meta-analysis was performed under fixed effect and random effect models whenever appropriate.
  
  
  RESULTS
  We found that the GG genotype is significantly more frequent in CAD patients than controls (adjusted p = 0.010; OR = 1.99, 95%; CI: 1.18 - 3.38). Additionally, through a meta-analysis, we showed that miR-499rs3746444 has a significant association with cardiovascular disease.
  
  
  CONCLUSIONS
  Our results suggest that miR-499-rs3746444-GG is associated with CAD susceptibility and development.
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Labbaf
authors.forename: Hamid
authors.initials: H
authors.lastname: Ghaedi
authors.forename: Behnam
authors.initials: B
authors.lastname: Alipoor
authors.forename: Mir Davood
authors.initials: MD
authors.lastname: Omrani
authors.forename: Faranak
authors.initials: F
authors.lastname: Kazerouni
authors.forename: Mehrnoush
authors.initials: M
authors.lastname: Shanaki
authors.forename: Maryam
authors.initials: M
authors.lastname: Ghaffarzadeh
authors.forename: Hossein
authors.initials: H
authors.lastname: Pashaiefar
authors.forename: Ali
authors.initials: A
authors.lastname: Rahimipour
mesh_terms: D016022:Case-Control Studies; D003324:Coronary Artery Disease; D020022:Genetic Predisposition to Disease; D006801:Humans; D035683:MicroRNAs; D020641:Polymorphism, Single Nucleotide
chemical_list: D035683:MicroRNAs