title: Partial biochemical characterization of Cu,Zn-superoxide dismutase extracted from eggs of hens (Gallus gallus domesticus).
abstract: Biochemical characteristics of Cu,Zn-SOD derived from hen egg white and egg yolk were determined, and compared with those of enzymes from erythrocytes of hens and SOD standard. The presence of dimer with a molecular weight of 33.38±0.34kDa, and pI of 6.30±0.15 was confirmed in samples of SOD extracted from egg yolk. Cu,Zn-SOD isolated from egg yolk had an optimum at pH 6. Average SOD activity in egg yolk was 98.5±19.5U·g-1while in egg white reached 6.1±0.8U·g-1. Changes in SOD activity of the egg yolk during its storage for 200days were also described. FTIR analysis confirmed that the enzymatic protein described in this study was SOD, while MALDI-TOF analysis confirmed only SOD from erythrocytes. Since eggs are a cheap and easily obtainable source of SOD, this enzymatic protein could be used in food, cosmetic or pharmaceutical industries.
authors.affiliation: Department of Animal Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland. Electronic address: wawrzykowski@up.lublin.pl.
authors.forename: J
authors.initials: J
authors.lastname: Wawrzykowski
authors.affiliation: Department of Animal Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kankofer
mesh_terms: D000818:Animals; D002645:Chickens; D004527:Egg Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004531:Eggs / Q000032:analysis*; D004795:Enzyme Stability; D005260:Female; D013058:Mass Spectrometry; D000072105:Superoxide Dismutase-1 / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004527:Egg Proteins; D000072105:Superoxide Dismutase-1
keywords: Activity; Characteristic; Chicken egg; Superoxide dismutase