title: Formation of a DNA Mini-Dumbbell with a Quasi-Type II Loop.
abstract: Quasi-loops, wherein the backbone has a discontinuous site in the loop, have been found to provide structural flexibility in the formation of DNA three-way junctions. Recently, a highly compact mini-dumbbell (MDB) structure composed of two adjacent CCTG or TTTA type II loops has been reported. Yet, it remains elusive if the presence of a quasi-loop will also facilitate the formation of an MDB. In this study, the possibility of whether an MDB can be formed containing a quasi-type II loop has been investigated. We first demonstrate that two adjacent CTTG type II loops can also form an MDB, which is thermodynamically more stable than those formed by CCTG and TTTA loops. Then, we systematically introduce quasi-type II loops with their discontinuous sites at different backbone positions in the CTTG MDB. Our results show that an MDB can be formed with a quasi-type II loop, in which there is a discontinuous site between the third thymine and the fourth guanine loop residues. The possible inclusion of a quasi-loop in MDBs expands the sequence criteria for the formation of MDBs by natural DNA sequences.
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong.
authors.forename: Pei
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong.
authors.forename: Sik Lok
authors.identifier: 0000-0001-5368-706X
authors.initials: SL
authors.lastname: Lam
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006860:Hydrogen Bonding; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
chemical_list: D004247:DNA