title: High Infiltration of Polarized CD163+Tumor-Associated Macrophages Correlates with Aberrant Expressions of CSCs Markers, and Predicts Prognosis in Patients with Recurrent Gastric Cancer.
abstract: Background:As the most predominant tumor-infiltrating immune cells, tumor-associated macrophages (TAMs) are associated with poor outcome in multiple solid cancers and play important roles in cancer progression. Cancer stem cells (CSCs) may account for metastasis and recurrence after cancer therapy. However, the association between TAMs and CSCs is not clarified in gastric cancer (GC). The aim of the present study was to evaluate the effects of TAMs on CSCs in GC and find out the risk factors to predict recurrence and prognosis.Material and methods:This study included consecutive 236 patients with histologically confirmed primary GC. TAMs marker CD163 and CSCs-related proteins were detected by immunohistochemistry (IHC) in GC tissues and their prognostic values were all investigated.Results:High expression of CD163+TAMs was found in patents with aggressive characteristics, especially for patents with recurrence. There existed a significant correlation between high expression of CD163 and CSCs-related markers in GC tissues. In patients with recurrence, high-expression of CD163 TAMs was an independent worse prognostic factor.Conclusion:High infiltration of TAMs was related to aggressive behavior, associated with aberrant expression of CSC markers, and an independent worse prognostic factor in GC. Targeting TAMs may be a potential treatment strategy for GC, including patients with recurrence.
authors.affiliation: Department of General Surgery, Drum Tower Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, China;; Department of General Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Pathology, 101th Hospital of PLA, Wuxi, Jiangsu Providence, China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, People' s Hospital of Anji, Huzhou, Zhejiang Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Tao-Hong
authors.initials: TH
authors.lastname: Pang
authors.affiliation: Department of General Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Shan-Ting
authors.initials: ST
authors.lastname: Gao
authors.affiliation: Department of Pathology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Xin-Yun
authors.initials: XY
authors.lastname: Xu
authors.affiliation: Department of Pathology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Drum Tower Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, China;; Department of General Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of General Surgery, Drum Tower Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, China;; Department of General Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of General Surgery, 101th Hospital of PLA, Wuxi, Jiangsu Province, China.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Lu
authors.affiliation: Department of General Surgery, 101th Hospital of PLA, Wuxi, Jiangsu Province, China.
authors.forename: Guo-Zhong
authors.initials: GZ
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Drum Tower Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, China;; Department of General Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Wen-Xian
authors.initials: WX
authors.lastname: Guan
authors.affiliation: Department of Gastroenterology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Xu
keywords: Cancer stem cells (CSCs); Gastric cancer; Prognosis.; Recurrent; Tumor-associated macrophages (TAMs)