title: Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells.
abstract: The present study describes the generation and the characterization of a stable cell line of neural stem cells derived from embryonic mouse hypothalamus. These cells (AC1) grow as an adherent culture in defined serum-free medium and express typical markers of neurogenic radial glia and of hypothalamic precursors. After prolonged expansion, AC1 cells may be efficiently induced to differentiate into neurons and astroglial cells in vitro and start to express some hormonal neuropeptides, like TRH, CRH, and POMC. Based on the capabilities of AC1 cells to be stably expanded and to develop neuroendocrine lineages in vitro, these cells might represent a novel tool to elucidate the mechanisms involved in the development of the hypothalamus and in the specific differentiation of neuroendocrine neurons.
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari; Sez. Biomedicina e Endocrinologia;  Università degli Studi di Milano; Milan, Italy; Centro Interuniversitario per lo studio delle Malattie della Riproduzione (CIRMAR); Università degli Studi di Milano; Milan, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Cariboni
authors.affiliation: Centro per la Biologia Integrata (CIBIO); Università degli Studi di Trento; Trento, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Conti
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari; Sez. Biomedicina e Endocrinologia;  Università degli Studi di Milano; Milan, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Andrè
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari; Sez. Biomedicina e Endocrinologia;  Università degli Studi di Milano; Milan, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Aprile
authors.affiliation: Centro per la Biologia Integrata (CIBIO); Università degli Studi di Trento; Trento, Italy.
authors.forename: Jacopo
authors.initials: J
authors.lastname: Zasso
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari; Sez. Biomedicina e Endocrinologia;  Università degli Studi di Milano; Milan, Italy; Centro Interuniversitario per lo studio delle Malattie della Riproduzione (CIRMAR); Università degli Studi di Milano; Milan, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Maggi
keywords: development; hypothalamus; neuroendocrinology; stem cells