title: MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma.
abstract: Interleukin enhancer binding factor 2 (ILF2) has been found to be markedly upregulated in pancreatic carcinoma and is involved in the pathogenesis of pancreatic carcinoma. Thus, ILF2 may be a potential target for therapy. Yet, the regulatory mechanisms of ILF2 in pancreatic carcinoma remain largely elusive. In the present study, we demonstrated that ILF2 functioned as an oncogene and regulated epithelial-mesenchymal transition (EMT)-associated genes in pancreatic carcinoma PANC-1 cells. MicroRNA-7 (miR-7) suppressed ILF2 mRNA expression and the protein level in PANC-1 cells. Contrary to ILF2, miRNA-7 functioned as a tumor-suppressor gene and negatively regulated EMT-associated genes in the PANC-1 cells. Curcumin, a polyphenol natural product isolated from the rhizome of the plant Curcuma longa, has emerged as a promising anticancer therapeutic agent. We found that treatment with curcumin increased miR-7 expression and suppressed ILF2 protein in the PANC-1 cells. Thus, we identified ILF2 as a new downstream target gene of curcumin. The results revealed that ILF2 is regulated by miR-7 and suggest that downregulation of miR-7 may be an important factor for the ILF2 overexpression in pancreatic carcinoma.
authors.affiliation: Department of Gastroenterology, 307 Hospital of PLA, Academy of Military Medical Science, Beijing 100071, P.R. China.
authors.forename: Yiliang
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Gastroenterology, 307 Hospital of PLA, Academy of Military Medical Science, Beijing 100071, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Gastroenterology, 307 Hospital of PLA, Academy of Military Medical Science, Beijing 100071, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Min
authors.affiliation: Department of Gastroenterology, 307 Hospital of PLA, Academy of Military Medical Science, Beijing 100071, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D051903:Nuclear Factor 45 Protein / Q000096:biosynthesis* / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D003474:Curcumin; D035683:MicroRNAs; D009363:Neoplasm Proteins; D051903:Nuclear Factor 45 Protein; D012334:RNA, Neoplasm; C494013:ILF2 protein, human; C549242:MIRN7-1 microRNA, human