title: Inhibition of multi-species oral biofilm by bromide doped bioactive glass.
abstract: Bioactive glass is an attractive biomaterial that has shown excellent osteogenic and angiogenic effects for oral bone repairing procedures. However, anti-biofilm potential related to such biomaterial has not been completely validated, mainly against multi-species biofilms involved in early tissue infections. The aim of the present study was to evaluate the anti-biofilm effect of 58 S bioactive glass embedding calcium bromide compounds at different concentrations. Bioactive glass free or containing 5, or 10 wt % CaBr2was synthesized by alkali sol-gel method and then characterized by physco-chemical analyses and scanning electron microscopy (SEM). Then, samples were tested by microbiological assays using optical density, real time q-PCR, and SEM. Bioactive glass particles showed accurate chemical composition and an angular shape with a bimodal size distribution ranging from 0.6 to 110 µm. The mean particle size was around 29 µm. Anti-biofilm effect was recorded for 5 wt % CaBr2-doped bioactive glass against S. mitis, V. parvula, P. gingivais, S. gordoni, A. viscosus, F, nucleatum, P. gingivais. F. nucleatum, and P. gingivalis. Such species are involved in the biofilm structure related to infections on hard and soft tissues in the oral cavity. The incorporation of calcium bromide into bioactive glass can be a strategy to enhance the anti-biofilm potential of bioactive glasses for bone healing and infection treatment. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1994-2003, 2017.
authors.affiliation: Center for Education and Research on Dental Implants (CEPID), Post-Graduate Program in Dentistry (PPGO), Department of Dentistry (ODT), Federal University of Santa Catarina (UFSC), Florianópolis/SC, 88040-900, Brazil.
authors.forename: M E
authors.initials: ME
authors.lastname: Galarraga-Vinueza
authors.affiliation: Center for Education and Research on Dental Implants (CEPID), Post-Graduate Program in Dentistry (PPGO), Department of Dentistry (ODT), Federal University of Santa Catarina (UFSC), Florianópolis/SC, 88040-900, Brazil.
authors.forename: B
authors.initials: B
authors.lastname: Passoni
authors.affiliation: Center for Education and Research on Dental Implants (CEPID), Post-Graduate Program in Dentistry (PPGO), Department of Dentistry (ODT), Federal University of Santa Catarina (UFSC), Florianópolis/SC, 88040-900, Brazil.
authors.forename: C A M
authors.initials: CAM
authors.lastname: Benfatti
authors.affiliation: Ceramic and Composite Materials Research Group (CERMAT), Department of Mechanical Engineering (EMC), Federal University of Santa Catarina, Florianópolis, 88040-900, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Mesquita-Guimarães
authors.affiliation: Ceramic and Composite Materials Research Group (CERMAT), Department of Mechanical Engineering (EMC), Federal University of Santa Catarina, Florianópolis, 88040-900, Brazil.
authors.forename: B
authors.initials: B
authors.lastname: Henriques
authors.affiliation: Center for Education and Research on Dental Implants (CEPID), Post-Graduate Program in Dentistry (PPGO), Department of Dentistry (ODT), Federal University of Santa Catarina (UFSC), Florianópolis/SC, 88040-900, Brazil.
authors.forename: R S
authors.initials: RS
authors.lastname: Magini
authors.affiliation: Ceramic and Composite Materials Research Group (CERMAT), Department of Mechanical Engineering (EMC), Federal University of Santa Catarina, Florianópolis, 88040-900, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Fredel
authors.affiliation: KU Leuven BIOMAT, Department of Oral Health Sciences, KU Leuven & Dentistry, University Hospitals Leuven, Kapucijnenvoer 7, 3000, Leuven, Belgium.
authors.forename: B V
authors.initials: BV
authors.lastname: Meerbeek
authors.affiliation: Department of Oral Health Sciences, KU Leuven & Dentistry, University Hospitals Leuven, Kapucijnenvoer 33, Leuven, B-3000, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Teughels
authors.affiliation: Center for Education and Research on Dental Implants (CEPID), Post-Graduate Program in Dentistry (PPGO), Department of Dentistry (ODT), Federal University of Santa Catarina (UFSC), Florianópolis/SC, 88040-900, Brazil.|Ceramic and Composite Materials Research Group (CERMAT), Department of Mechanical Engineering (EMC), Federal University of Santa Catarina, Florianópolis, 88040-900, Brazil.
authors.forename: J C M
authors.identifier: http://orcid.org/0000-0001-7999-4009
authors.initials: JCM
authors.lastname: Souza
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D018407:Bacterial Physiological Phenomena; D018441:Biofilms / Q000254:growth & development*; D001965:Bromides / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D009055:Mouth / Q000382:microbiology*
chemical_list: D001965:Bromides; C448994:bioactive glass 58S
keywords: anti-biofilm; bioactive glass; bone healing; bone infection; bromide; sol-gel synthesis