title: Field-assisted paper spray mass spectrometry for therapeutic drug monitoring: 1. the case of imatinib in plasma.
abstract: The field-assisted paper spray (FAPS) - mass spectrometric method has been employed to quantify the imatinib (IMT) plasma levels in treated patients. The quantitative measurements have been performed on the collisionally generated fragment at m/z 394 of the protonated molecules of IMT and deuterated IMT (d3-IMT), used as internal standard. The FAPS-tandem mass spectrometry (MS/MS) method exhibits some limitations, because of the high number of operative parameters that need to be carefully controlled. For this aim, papers of different geometry, thickness, and porosity were tested. To obtain a more focalized and intense electrical field, a stainless steel needle was mounted axially and placed at 4 kV voltage. The variability observed in the measurements was ascribed either to the inter-individual variability (e.g. the concomitant presence of other compounds such as proteins, lipids, drugs and/or salts in the plasma of different patients) or to the uncontrollable variables in the instrumental set-up (e.g. sample deposition, changes in paper spray conditions). Furthermore, the manual sample deposition and solvent dripping strongly affects the measure reproducibility. Despite this, it is interesting to observe that, once applied in blind on 24 real plasma samples, FAPS-MS/MS led to results analogous to those obtained by the well-consolidated liquid chromatography-MS/MS, even if the mean coefficient of variation % (CV%) values of 20.4% and 2.6% were observed for the two methods, respectively. In conclusion, despite CV values are relatively high, it is worth noting that the FAPS-MS/MS method is much more straightforward, rapid and economical than the liquid chromatography-MS/MS one, and it appears therefore very promising for applications where a high precision is not always a required task, as e.g. in some cases of therapeutic drug monitoring. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Nano-inspired Biomedicine Lab, Institute of Paediatric Research - Città della Speranza, Corso Stati Uniti 4, Padova, 35127, Italy.
authors.forename: S
authors.identifier: 0000-0001-6956-7943
authors.initials: S
authors.lastname: D'Aronco
authors.affiliation: Department of Chemical Sciences, University of Padova, via Marzolo 1, Padova, 35131, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Dall'Armi
authors.affiliation: Nano-inspired Biomedicine Lab, Institute of Paediatric Research - Città della Speranza, Corso Stati Uniti 4, Padova, 35127, Italy.
authors.forename: S
authors.identifier: 0000-0001-6602-5153
authors.initials: S
authors.lastname: Crotti
authors.affiliation: Nano-inspired Biomedicine Lab, Institute of Paediatric Research - Città della Speranza, Corso Stati Uniti 4, Padova, 35127, Italy.
authors.forename: E
authors.identifier: 0000-0003-0591-8155
authors.initials: E
authors.lastname: Calandra
authors.affiliation: Nano-inspired Biomedicine Lab, Institute of Paediatric Research - Città della Speranza, Corso Stati Uniti 4, Padova, 35127, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Traldi
authors.affiliation: Department of Chemical Sciences, University of Padova, via Marzolo 1, Padova, 35131, Italy.
authors.forename: V
authors.identifier: 0000-0001-6108-746X
authors.initials: V
authors.lastname: Di Marco
authors.affiliation: Experimental and Clinical Pharmacology Unit, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, Via Franco Gallini 2, Aviano, 33081, PN, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Buonadonna
authors.affiliation: Experimental and Clinical Pharmacology Unit, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, Via Franco Gallini 2, Aviano, 33081, PN, Italy.
authors.forename: G
authors.identifier: 0000-0003-3619-7709
authors.initials: G
authors.lastname: Corona
authors.affiliation: Experimental and Clinical Pharmacology Unit, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, Via Franco Gallini 2, Aviano, 33081, PN, Italy.
authors.forename: L
authors.identifier: 0000-0003-1077-5873
authors.initials: L
authors.lastname: Giodini
authors.affiliation: Experimental and Clinical Pharmacology Unit, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, Via Franco Gallini 2, Aviano, 33081, PN, Italy.
authors.forename: E
authors.identifier: 0000-0001-6333-2369
authors.initials: E
authors.lastname: Marangon
authors.affiliation: Experimental and Clinical Pharmacology Unit, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, Via Franco Gallini 2, Aviano, 33081, PN, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Posocco
authors.affiliation: Experimental and Clinical Pharmacology Unit, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, Via Franco Gallini 2, Aviano, 33081, PN, Italy.
authors.forename: G
authors.identifier: 0000-0002-5323-4762
authors.initials: G
authors.lastname: Toffoli
authors.affiliation: Nano-inspired Biomedicine Lab, Institute of Paediatric Research - Città della Speranza, Corso Stati Uniti 4, Padova, 35127, Italy.|Surgical Clinic, Department of Surgical, Oncological and Gastroenterological Sciences, University of Padova, Via Nicolò Giustiniani 2, Padova, 35128, Italy.
authors.forename: M
authors.identifier: 0000-0001-7578-1233
authors.initials: M
authors.lastname: Agostini
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D000068877:Imatinib Mesylate / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D000068877:Imatinib Mesylate
keywords: comparison PS and LC-MS/MS; imatinib; imatinib in plasma; paper spray; therapeutic drug monitoring