title: Protective effect of Aster tataricus extract on retinal damage on the virtue of its antioxidant and anti-inflammatory effect in diabetic rat.
abstract: Effect of Aster tataricus (AT) was estimated on the retinal injury in diabetic rats by its antioxidant and anti-inflammatory activity. Streptozotocin (STZ) was used to induce diabetes at a dose of 60mg/kg, i.p. and blood glucose was estimated to confirm the diabetic rats. All the animals were separated in to 5 different groups (n=10) such as control, diabetic retinopathy (DR) receives saline solution, and AT treated group receives AT (100, 200 and 400mg/kg) for the duration of 8 week. After treatment protocol period blood glucose and HbA1c% was estimated in the blood sample of diabetic rats. Retinal tissue was isolated for the fundus photography and retinal vessel diameter, retinal vascular permeability and leukocytosis were estimated. Moreover in the retinal tissue homogenate oxidative stress parameters such as superoxide dismutase (SOD), glutathione peroxidase (GSH) and catalase (CAT) and concentration of cytokines (TNFα, IL10) was estimated. Result of the study suggested that root extract of AT contain rich amount of polyphenol in it which significantly reduces the body weight and concentration of glucose in blood in diabetic rats. Fundus photography suggested that AT extract attenuates the structure and functional abnormalities that develops due to diabetes. Retinal leukocytosis and vascular permeability was significantly decreases in AT treated group than DR group. There was significant increase in the activity of GSH, CAT and SOD in AT treated group than DR group. Moreover AT also attenuates the altered concentration of TNFα, IL10 and NF-κB in the retina of STZ induced diabetic rat. Thus present study concludes that root extract of AT effectively manages the diabetic retinopathy by controlling the blood glucose and also by attenuating the altered oxidative stresss and inflammatory mediators such as TNFα, IL10 and NF-κB in the retina of STZ induced diabetic rat.
authors.affiliation: Department of Ophthalmology, The Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Du
authors.affiliation: Department of Ophthalmology, The Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, The Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, PR China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Yao
authors.affiliation: Queensland Brain Institute, The University of Queensland,Upland Rd.Bldg.#79,Room 431, Brisbane, QLD,4072, Australia. Electronic address: zhitaohu134@hotmail.com.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D031173:Aster Plant / Q000737:chemistry* / Q000633:toxicity; D001786:Blood Glucose / Q000032:analysis; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D003930:Diabetic Retinopathy / Q000000981:diagnostic imaging / Q000473:pathology / Q000517:prevention & control*; D005451:Fluorescein Angiography; D007004:Hypoglycemic Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology* / Q000633:toxicity; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001786:Blood Glucose; D016207:Cytokines; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D059808:Polyphenols; D020011:Protective Agents
keywords: Antioxidant; Aster tataricus; Cytokines; Diabetic retinopathy; Fundus photography