title: Development of high throughput LC/MS/MS method for analysis of perfluorooctanoic acid from serum, suitable for large-scale human biomonitoring.
abstract: A simple method for determination of perfluorooctanoic acid (PFOA) from human serum by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and utilizing high-throughput sample preparation was developed by New York State Public Health Emergency Preparedness Laboratory and used for biomonitoring studies. 50μl of serum was mixed with a 0.4ml of acetonitrile containing isotopically labeled internal standard, followed by phospholipid removal/protein precipitation. The extract was partially dried and analyzed by LC/MS/MS. The linear range of PFOA analysis was 0.5-100ng/ml. LLOQ was selected as 0.5ng/ml. The method was validated following APHL guidelines for LRN-C laboratories and about 6000 specimens were successfully prepared and analyzed using this simple LC/MS method. Due to sample preparation simplicity, the presented method can be used in large-scale clinical testing, such as public health surveillance studies.
authors.affiliation: Wadsworth Center, New York State Department of Health, Division of Environmental Health Sciences, Albany, NY 12201, United States. Electronic address: eduard.rogatsky@health.ny.gov.
authors.forename: Eduard
authors.initials: E
authors.lastname: Rogatsky
authors.affiliation: Wadsworth Center, New York State Department of Health, Division of Environmental Health Sciences, Albany, NY 12201, United States.
authors.forename: Colleen
authors.initials: C
authors.lastname: O'Hehir
authors.affiliation: Wadsworth Center, New York State Department of Health, Division of Environmental Health Sciences, Albany, NY 12201, United States.
authors.forename: James
authors.initials: J
authors.lastname: Daly
authors.affiliation: Wadsworth Center, New York State Department of Health, Division of Environmental Health Sciences, Albany, NY 12201, United States.
authors.forename: Amelie
authors.initials: A
authors.lastname: Tedesco
authors.affiliation: Wadsworth Center, New York State Department of Health, Division of Environmental Health Sciences, Albany, NY 12201, United States.
authors.forename: Richard
authors.initials: R
authors.lastname: Jenny
authors.affiliation: Wadsworth Center, New York State Department of Health, Division of Environmental Health Sciences, Albany, NY 12201, United States.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Aldous
mesh_terms: D002210:Caprylates / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005466:Fluorocarbons / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002210:Caprylates; D005466:Fluorocarbons; C023036:perfluorooctanoic acid
keywords: High throughput; LC/MS; PFOA; Sample preparation