title: Elaboration and evaluation of alginate foam scaffolds for soft tissue engineering.
abstract: Controlling microarchitecture in polymer scaffolds is a priority in material design for soft tissue applications. This paper reports for the first time the elaboration of alginate foam-based scaffolds for mesenchymal stem cell (MSC) delivery and a comparative study of various surfactants on the final device performance. The use of surfactants permitted to obtain highly interconnected porous scaffolds with tunable pore size on surface and in cross-section. Their mechanical properties in compression appeared to be adapted to soft tissue engineering. Scaffold structures could sustain MSC proliferation over 14 days. Paracrine activity of scaffold-seeded MSCs varied with the scaffold structure and growth factors release was globally improved in comparison with control alginate scaffolds. Our results provide evidence that exploiting different surfactant types for alginate foam preparation could be an original method to obtain biocompatible scaffolds with tunable architecture for soft tissue engineering.
authors.affiliation: CIRIMAT, Université de Toulouse, CNRS, INPT, Université Toulouse 3 Paul Sabatier, Faculté de Pharmacie, F-31062 Toulouse, France; INSERM, UMR 1048, F-31432 Toulouse, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Ceccaldi
authors.affiliation: CIRIMAT, Université de Toulouse, CNRS, INPT, Université Toulouse 3 Paul Sabatier, Faculté de Pharmacie, F-31062 Toulouse, France; INSERM, UMR 1048, F-31432 Toulouse, France.
authors.forename: Raya
authors.initials: R
authors.lastname: Bushkalova
authors.affiliation: INSERM, UMR 1048, F-31432 Toulouse, France; Université de Toulouse, UPS, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cussac
authors.affiliation: Université de Toulouse, CIRIMAT, UPS-INPT-CNRS, F-31062 Toulouse, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Duployer
authors.affiliation: Université de Toulouse, CIRIMAT, UPS-INPT-CNRS, F-31062 Toulouse, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Tenailleau
authors.affiliation: EFS, Laboratoire de thérapie cellulaire, F-31027 Toulouse, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bourin
authors.affiliation: INSERM, UMR 1048, F-31432 Toulouse, France; Université de Toulouse, UPS, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse, France; CHU Toulouse, Pôle Pharmacie, F-31432 Toulouse, France.
authors.forename: Angelo
authors.initials: A
authors.lastname: Parini
authors.affiliation: INSERM, UMR 1048, F-31432 Toulouse, France; Université de Toulouse, UPS, Faculté des Sciences Pharmaceutiques, F-31062 Toulouse, France; CHU Toulouse, Pôle Pharmacie, F-31432 Toulouse, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Sallerin
authors.affiliation: CIRIMAT, Université de Toulouse, CNRS, INPT, Université Toulouse 3 Paul Sabatier, Faculté de Pharmacie, F-31062 Toulouse, France. Electronic address: sophie.fullana-girod@univ-tlse3.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Girod Fullana
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Alginate; Foam-based scaffolds; Mesenchymal stem cells; Soft tissue engineering; Surfactants