title: Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing-thawing of semen from capuchin monkey.
abstract: We aimed to evaluate the effect of supplementation of ACP-118® extender with the antioxidant catalase (10 and 50 µg/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 µg/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.
authors.affiliation: Laboratory of Amazon Animal Biotechnology and Medicine,Faculty of Veterinary Medicine,Federal University of Pará,Belém,Pará,Brazil.
authors.forename: Danuza L
authors.initials: DL
authors.lastname: Leão
authors.affiliation: Laboratory of Amazon Animal Biotechnology and Medicine,Faculty of Veterinary Medicine,Federal University of Pará,Belém,Pará,Brazil.
authors.forename: Adriel B
authors.initials: AB
authors.lastname: Brito
authors.affiliation: Laboratory of Amazon Animal Biotechnology and Medicine,Faculty of Veterinary Medicine,Federal University of Pará,Belém,Pará,Brazil.
authors.forename: Stefânia A
authors.initials: SA
authors.lastname: Miranda
authors.affiliation: Laboratory of Amazon Animal Biotechnology and Medicine,Faculty of Veterinary Medicine,Federal University of Pará,Belém,Pará,Brazil.
authors.forename: Karol G
authors.initials: KG
authors.lastname: Oliveira
authors.affiliation: Laboratory of Amazon Animal Biotechnology and Medicine,Faculty of Veterinary Medicine,Federal University of Pará,Belém,Pará,Brazil.
authors.forename: Débora V C
authors.initials: DVC
authors.lastname: Almeida
authors.affiliation: Federal University of Pará,Laboratory of Wild Animal Biology and Medicine,BR 316 Km 61,CEP 68740-970,Castanhal,Pará,Brazil.
authors.forename: Regiane R
authors.initials: RR
authors.lastname: Santos
authors.affiliation: Laboratory of Amazon Animal Biotechnology and Medicine,Faculty of Veterinary Medicine,Federal University of Pará,Belém,Pará,Brazil.
authors.forename: Sheyla F S
authors.initials: SFS
authors.lastname: Domingues
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002428:Cebus; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D008297:Male; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects*
chemical_list: D003451:Cryoprotective Agents; D002374:Catalase
keywords: Antioxidant; Cell membrane; Monkey; Semen