title: Enhancement of porcine intramuscular fat content by overexpression of the cytosolic form of phosphoenolpyruvate carboxykinase in skeletal muscle.
abstract: Intramuscular fat (IMF) content has been generally recognized as a desirable trait in pork meat because of its positive effect on eating quality. An effective approach to enhance IMF content in pork is the generation of transgenic pigs. In this study, we used somatic cell nuclear transfer (SCNT) to generate cloned pigs exhibiting ectopic expression of phosphoenolpyruvate carboxykinase (PEPCK-C) driven by an α-skeletal-actin gene promoter, which was specifically expressed in skeletal muscle. Using qRT-PCR and Western blot analysis, we demonstrated that PEPCK-C was functionally expressed and had a significant effect on total fatty acid content in the skeletal muscle of the transgenic pigs, while the n-6/n-3 polyunsaturated fatty acid (PUFA) ratio showed no difference between transgenic and control pigs. Thus, genetically engineered PEPCK-Cmuspigs may be an effective solution for the production of IMF-enriched pork.
authors.affiliation: Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 101 Longmian Avenue, Nanjing, 211166, People's Republic of China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 101 Longmian Avenue, Nanjing, 211166, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Heping District, Qixiangtai Road, Tianjin 300070, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Huaian First Hospital Affiliated with Nanjing Medical University, Huai'an, People's Republic of China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Comparative Medicine and Center of Laboratory Animals, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Weiwang
authors.initials: W
authors.lastname: Gu
authors.affiliation: State key laboratory of medicinal chemical biology, Key laboratory of bioactive materials, Ministry of education, Tianjin key laboratory of protein sciences and College of life sciences, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Zhaoting
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State key laboratory of medicinal chemical biology, Key laboratory of bioactive materials, Ministry of education, Tianjin key laboratory of protein sciences and College of life sciences, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Chen
authors.affiliation: Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, 518035, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Mou
authors.affiliation: Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 101 Longmian Avenue, Nanjing, 211166, People's Republic of China.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 101 Longmian Avenue, Nanjing, 211166, People's Republic of China.
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 101 Longmian Avenue, Nanjing, 211166, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D030801:Animals, Genetically Modified; D003600:Cytosol; D015870:Gene Expression; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D008460:Meat / Q000592:standards; D018482:Muscle, Skeletal / Q000378:metabolism*; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D013552:Swine
chemical_list: D019764:Phosphoenolpyruvate Carboxykinase (ATP)