title: Gene expression profiling of circulating CD133+cells of hepatocellular carcinoma patients associated with HCV infection.
abstract: OBJECTIVE
  Identifying the genetic expression profile of CD133+cells from HCC patients compared to CD133+cells from healthy volunteers that may contribute in hepatocarcinogenesis process.
  
  
  METHODS
  Circulating CD133+cells were sorted from the peripheral blood of HCC patients as well as from healthy volunteers using magnetic activated cell sorting. The differential expression profile of stem cell related genes was performed using the Stem Cell PCR profiling assay.
  
  
  RESULTS
  Data analysis of stem cells related genes in CD133+cells of the HCC group compared to the control group showed that; CCND2, COL1A1, CTNNA1, DLL3, JAG1, KRT15, MYC, NOTCH2, T and TERT were up-regulated (fold change=80, 68.6, 6.67, 7.22, 3.8, 15.2, 14.5, 105.6, 26.6 and 99 respectively while only CD3D was down-regulated (fold change=0.055) in HCC patients. However, after application of Beferroni correction to adjust P-value; KRT15 was the only gene that was significantly over expressed in CD133+cells of HCC compared to control group (P-value=0.012).
  
  
  CONCLUSIONS
  KRT15 can be used to differentiate between circulating CD133+cells from HCC group and control group. However, further study may be needed to confirm on the protein level.
authors.affiliation: Molecular Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. Electronic address: ncizekri@yahoo.com.
authors.forename: Abdel-Rahman N
authors.initials: AN
authors.lastname: Zekri
authors.affiliation: Molecular Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Enas R
authors.initials: ER
authors.lastname: El-Sisi
authors.affiliation: Molecular Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Zeinab F
authors.initials: ZF
authors.lastname: Abdallah
authors.affiliation: Surgery Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Alaa
authors.initials: A
authors.lastname: Ismail
authors.affiliation: Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Barakat Barakat
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000473:pathology*; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D006526:Hepatitis C / Q000150:complications*; D006801:Humans; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D008111:Liver Function Tests; D008113:Liver Neoplasms / Q000209:etiology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism; D059467:Transcriptome
chemical_list: D000071916:AC133 Antigen; D015415:Biomarkers
keywords: CD133(+) cells; HCV; Hepatocellular carcinoma