title: Enzymatic synthesis of lysophosphatidylcholine with n-3 polyunsaturated fatty acid from sn-glycero-3-phosphatidylcholine in a solvent-free system.
abstract: The n-3 polyunsaturated fatty acids (PUFA)-rich lysophosphatidylcholine (LPC) was successfully synthesized by Thermomyces lanuginosus lipase (TL IM)-catalyzed esterification of glycerylphosphorylcholine (GPC) and n-3 PUFA-rich fatty acids in a solvent-free system. Effects of reaction temperature, enzyme loading and substrate mole ratio on the yield of LPC and incorporation of n-3 PUFA were evaluated. The acyl-specificities of five enzymes were tested for direct esterification of n-3 PUFA, and Lipozyme TL IM was found to be more effective than others for production of LPC with n-3 PUFA. Substrate mole ratio and reaction temperature, however, had no significant effect on the incorporation. The maximal yield of LPC was obtained under the following conditions: temperature 45°C, enzyme loading 15% by weight and substrate mole ratio (GPC/n-3 PUFA) 1:20. Furthermore, the composition of products were further investigated in the study. The 1-acyl-sn-glycero-3-lysophosphatidylcholine (2-LPC) was predominant in the mixtures at early stages of reaction, whereas less increment of 2-acyl-sn-glycero-3-lysophosphatidylcholine (1-LPC) and PC was observed at later stages.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: fs-lauyang@hotmail.com.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: zhangqinouc@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: foodgyl@outlook.com.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: fs-liujunyi@outlook.com.
authors.forename: Junyi
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: xujie9@ouc.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: lizhaojie@ouc.edu.cn.
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: jfwang@ouc.edu.cn.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: wangyuming@ouc.edu.cn.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: xuech@ouc.edu.cn.
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D015525:Fatty Acids, Omega-3 / Q000737:chemistry*; D008244:Lysophosphatidylcholines / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*
chemical_list: D015525:Fatty Acids, Omega-3; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines
keywords: Docosahexaenoic acid; Lipozyme TL IM; Lysophosphatidylcholine; n−3 polyunsaturated fatty acids; sn-Glycero-3-phosphatidylcholine