title: Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest.
abstract: OBJECTIVE
  The aim of this study was the coproduction in a single strain of the Gram-negative bacteriocin colicin V with other bacteriocins from lactic acid bacteria (LAB).
  
  
  RESULTS
  Colicin V was expressed in Lactococcus and Enterococcus strains by replacing the colicin V leader peptide by the leader peptide and promoter of d-alanyl-d-alanine carboxypeptidase from Lactobacillus reuteri CECT925 in pNZ8048 (pNZ:LR-colV). The antimicrobial activity of colicin V against the indicator organism Escherichia coli DH5α in transformed strains was checked by agar diffusion assay and SDS-PAGE analysis.
  
  
  CONCLUSIONS
  Lactococcus and Enterococcus transformed with pNZ:LR-colV were able to coproduce colicin V at high levels together with other LAB bacteriocins such as nisin A, nisin Z, lacticin 481 or enterocins A and B, obtaining broad-spectrum activity strains with large potential applications.
  
  
  CONCLUSIONS
  The construction showed in this work could be used for the heterologous expression of other bacteriocins active against Gram-negative bacteria or wide-spectrum bacteriocins from LAB.
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
authors.forename: S
authors.identifier: http://orcid.org/0000-0003-2729-219X
authors.initials: S
authors.lastname: Langa
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
authors.forename: J L
authors.identifier: http://orcid.org/0000-0002-8548-0183
authors.initials: JL
authors.lastname: Arqués
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Medina
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Landete
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D003087:Colicins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D016983:Enterococcus / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects; D007218:Industrial Microbiology; D019344:Lactic Acid / Q000378:metabolism*; D017045:Lactococcus / Q000737:chemistry / Q000378:metabolism*; D021382:Protein Sorting Signals
chemical_list: D000900:Anti-Bacterial Agents; D003087:Colicins; D021382:Protein Sorting Signals; D019344:Lactic Acid
keywords: Enterococcus; Lactococcus; bacteriocins; colicin V; coproduction; heterologous production; lactic acid bacteria