title: Self-interaction of Citrus tristeza virus p33 protein via N-terminal helix.
abstract: Citrus tristeza virus (CTV), the most economically important viral pathogen of citrus, encodes a unique protein, p33. CTV p33 shows no similarity with other known proteins, yet plays an important role in viral pathogenesis: it extends the virus host range and mediates virus ability to exclude superinfection by other variants of the virus. Previously we demonstrated that p33 is an integral membrane protein and appears to share characteristics of viral movement proteins. In this study, we show that the p33 protein self-interacts in vitro and in vivo using co-immunoprecipitation, yeast two hybrid, and bimolecular fluorescence complementation assays. Furthermore, a helix located at the N-terminus of the protein is required and sufficient for the protein self-interaction.
authors.affiliation: University of Florida, Plant Pathology Department, Gainesville, FL 32611, USA.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Kang
authors.affiliation: University of Florida, Plant Pathology Department, Gainesville, FL 32611, USA.
authors.forename: Thi Nguyet Minh
authors.initials: TNM
authors.lastname: Dao
authors.affiliation: University of Florida, Plant Pathology Department, Gainesville, FL 32611, USA.
authors.forename: Ok-Kyung
authors.initials: OK
authors.lastname: Kim
authors.affiliation: University of Florida, Plant Pathology Department, Gainesville, FL 32611, USA. Electronic address: svetlana@ufl.edu.
authors.forename: Svetlana Y
authors.initials: SY
authors.lastname: Folimonova
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002957:Citrus / Q000821:virology; D003001:Cloning, Molecular; D017790:Closterovirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D016679:Genome, Viral; D058507:Host Specificity; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D010935:Plant Diseases / Q000821:virology; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins
keywords: Citrus tristeza virus; Plant virus; Protein–protein interaction