title: Transcriptional profiling of host cell responses to encephalomyocarditis virus (EMCV).
abstract: Encephalomyocarditis virus (EMCV) has been discovered on pig farms worldwide and can cause myocarditis in piglets and reproductive failure in sows. However, little is known about the host transcriptional responses to infection and host-pathogen interactions.
  
  
  
  In this study, transcription profiling was performed by Illumina RNA-Sequencing (RNA-seq) to identify EMCV induced differentially expressed genes in BHK-21 cells at serial time points (12, 24, and 30 h post infection (hpi)), using mock infected cells as control.
  
  
  
  We identified 237, 241, and 207 differentially expressed genes (DEGs) respectively, majority of which were up-regulated. A large number of DEGs clustered into host defense, cellular signaling and metabolism categories. Moreover, short time series expression analysis revealed that 12 hpi was an important time point for expression change, indicating host virus resistance.
  
  
  
  This RNA-seq analysis provides the first data for understanding the network of virus host interactions under EMCV infection in vitro, and for identifying host components which involved in the virus infection course.
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.|School of Life Science and Bioengineering, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wei
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Li
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.|School of Life Science and Bioengineering, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
authors.forename: Qiongyi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Animal cell Engineering & Technology Research Center of Gansu, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
authors.forename: Zhongren
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Animal cell Engineering & Technology Research Center of Gansu, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Bai
authors.affiliation: Animal cell Engineering & Technology Research Center of Gansu, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Qiao
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China. fengruofei@xbmu.edu.cn.|Animal cell Engineering & Technology Research Center of Gansu, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China. fengruofei@xbmu.edu.cn.
authors.forename: Ruofei
authors.identifier: 0000-0001-9647-2827
authors.initials: R
authors.lastname: Feng
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004686:Encephalomyocarditis virus / Q000276:immunology* / Q000472:pathogenicity*; D004847:Epithelial Cells / Q000821:virology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D017423:Sequence Analysis, RNA; D013997:Time Factors
keywords: Encephalomyocarditis virus (EMCV); Host transcriptional responses; Transcriptome analysis