title: Transcriptional Reprogramming at Genome-Scale ofLactobacillus plantarumWCFS1 in Response to Olive Oil Challenge.
abstract: Dietary fats may exert selective pressures onLactobacillusspecies, however, knowledge on the mechanisms of adaptation to fat stress in these organisms is still fragmentary. This study was undertaken to gain insight into the mechanisms of adaptation ofLactobacillus plantarumWCFS1 to olive oil challenge by whole genome transcriptional profiling using DNA microarrays. A set of 230 genes were differentially expressed byL. plantarumWCFS1 to respond to this vegetable oil. This response involved elements typical of the stringent response, as indicated by the induction of genes involved in stress-related pathways and downregulation of genes related to processes associated with rapid growth. A set of genes involved in the transport and metabolism of compatible solutes were downregulated, indicating that this organism does not require osmoprotective mechanisms in presence of olive oil. The fatty acid biosynthetic pathway was thoroughly downregulated at the transcriptional level, which coincided with a diminished expression of genes controlled by this pathway in other organisms and that are required for the respiratory function, pyruvate dehydrogenase activity, RNA processing and cell size setting. Finally, a set of genes involved in host-cell signaling byL. plantarumwere differentially regulated indicating that olive oil can influence the expression of metabolic traits involved in the crosstalk between this bacterium and the host.
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición - Consejo Superior de Investigaciones Cientificas Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Esteban-Torres
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición - Consejo Superior de Investigaciones Cientificas Madrid, Spain.
authors.forename: Inés
authors.initials: I
authors.lastname: Reverón
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición - Consejo Superior de Investigaciones Cientificas Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Plaza-Vinuesa
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición - Consejo Superior de Investigaciones Cientificas Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: de Las Rivas
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición - Consejo Superior de Investigaciones Cientificas Madrid, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Muñoz
authors.affiliation: Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición - Consejo Superior de Investigaciones Cientificas Madrid, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: López de Felipe
keywords: Lactobacillus plantarum; fatty acid biosynthesis; olive oil; stringent response; transcriptomics