title: Inhibition of MRSA and of Clostridium difficile by durancin 61A: synergy with bacteriocins and antibiotics.
abstract: The aim of this study was to evaluate the efficacy of durancin 61A alone or in combination with nisin, pediocin PA-1, reuterin, microcin J25, vancomycin or tetracycline as an inhibitor of resistant clinical pathogens and to shed light on its mode of action.
  
  
  
  Durancin and reuterin were effective inhibitors of Clostridium difficile, vancomycin-resistant Enterococcus faecium and methicillin-resistant Staphylococcus aureus. The combination of durancin and reuterin was highly synergistic against C. difficile (fractional inhibitory concentration index = 0.2). Durancin/vancomycin combination was synergistic against S. aureus ATCC®700699 (fractional inhibitory concentration index = 0.3). Conclusion & future perspective: Durancin 61A alone or combined with other bacteriocins or antibiotics may therefore provide a possible therapeutic option for the treatment of infections by these pathogens.
authors.affiliation: STELA Dairy Research Centre, Institute of Nutrition & Functional Foods, Université Laval, G1K 7P4 Québec, QC, Canada.|Unité Protéomie Fonctionnelle et Potentiel Nutraceutique de la Biodiversité de Tunisie, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El Manar, Tunisie.
authors.forename: Hasna
authors.initials: H
authors.lastname: Hanchi
authors.affiliation: STELA Dairy Research Centre, Institute of Nutrition & Functional Foods, Université Laval, G1K 7P4 Québec, QC, Canada.|Present address: Department of Soil Sciences and Agri-Food Engineering, Université Laval, Quebec, QC G1V 0A6, Canada.
authors.forename: Riadh
authors.initials: R
authors.lastname: Hammami
authors.affiliation: Centre de Recherche en Infectiologie de l'Université Laval, CHUQ, Pavillon CHUL 2705 boul. Laurier, Ste-Foy, Quebec G1V 4G2, Canada.
authors.forename: Hélène
authors.initials: H
authors.lastname: Gingras
authors.affiliation: Unité Protéomie Fonctionnelle et Potentiel Nutraceutique de la Biodiversité de Tunisie, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El Manar, Tunisie.
authors.forename: Rim
authors.initials: R
authors.lastname: Kourda
authors.affiliation: Centre de Recherche en Infectiologie de l'Université Laval, CHUQ, Pavillon CHUL 2705 boul. Laurier, Ste-Foy, Quebec G1V 4G2, Canada.
authors.forename: Michel G
authors.initials: MG
authors.lastname: Bergeron
authors.affiliation: Unité Protéomie Fonctionnelle et Potentiel Nutraceutique de la Biodiversité de Tunisie, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El Manar, Tunisie.
authors.forename: Jeannette
authors.initials: J
authors.lastname: Ben Hamida
authors.affiliation: Centre de Recherche en Infectiologie de l'Université Laval, CHUQ, Pavillon CHUL 2705 boul. Laurier, Ste-Foy, Quebec G1V 4G2, Canada.
authors.forename: Marc
authors.initials: M
authors.lastname: Ouellette
authors.affiliation: STELA Dairy Research Centre, Institute of Nutrition & Functional Foods, Université Laval, G1K 7P4 Québec, QC, Canada.
authors.forename: Ismail
authors.initials: I
authors.lastname: Fliss
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D016360:Clostridioides difficile / Q000187:drug effects* / Q000502:physiology; D004357:Drug Synergism; D016983:Enterococcus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D009561:Nisin; D014640:Vancomycin; C102526:nisin A; D013752:Tetracycline
keywords: antibiotics; bacteriocins; clinical pathogens; drug-resistance; synergy