title: Prostaglandin E2promotes BeWo spheroids implantation in RL95-2 cell monolayers.
abstract: OBJECTIVE
  Integrin αvβ3 (ITG αvβ3) participates in the process of implantation between the embryo and the endometrium. This study investigated the effects of prostaglandin E2(PGE2) on endometrial receptivity and implantation efficiency of the embryo, and their possible mechanisms.
  
  
  METHODS
  Quantitative real-time reverse transcription PCR (qRT-PCR) and western blotting were used to detect the changes in mRNA and protein levels of ITG αvβ3 in RL95-2 cells after administering PGE2. BeWo trophoblast cells and RL95-2 endometrial epithelial cells were used to establish an in vitro model, which was used to observe the adhesion rate and spreading efficiency between BeWo spheroids and RL95-2 cell monolayers after pretreatment with different concentrations of PGE2.
  
  
  RESULTS
  PGE2at 200 nM increased the mRNA and protein levels of ITG αv significantly (p < 0.05); 100 nM PGE2increased the mRNA and protein levels of ITG β3 significantly (p < 0.05). PGE2at 200 nM increased significantly the adhesion and spreading efficiency of BeWo spheres to RL95-2 cell monolayers.
  
  
  CONCLUSIONS
  An appropriate concentration of PGE2might increase the expression of ITG αvβ3, which would, promote embryo adhesion and spreading efficiency. This study provides further evidence that increased expression of ITG αvβ3 might promote implantation by improving endometrial receptivity.
authors.affiliation: a Obstetrical Department, The First Affiliated Hospital of Jinan University , Guangzhou , Guangdong , PR China.
authors.forename: Xiaman
authors.initials: X
authors.lastname: Huang
authors.affiliation: a Obstetrical Department, The First Affiliated Hospital of Jinan University , Guangzhou , Guangdong , PR China.
authors.forename: Haizhi
authors.initials: H
authors.lastname: Liu
authors.affiliation: a Obstetrical Department, The First Affiliated Hospital of Jinan University , Guangzhou , Guangdong , PR China.
authors.forename: Ruiman
authors.initials: R
authors.lastname: Li
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D015232:Dinoprostone / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D039302:Integrin alphaVbeta3; D015232:Dinoprostone
keywords: Embryo implantation; integrin αvβ3; prostaglandin E