title: TLR7 Deficiency Leads to TLR8 Compensative Regulation of Immune Response against JEV in Mice.
abstract: Japanese encephalitis virus (JEV) is a highly fatal pathogen to human beings. Toll-like receptor 7 (TLR7) plays a role as the first host defense against most single-stranded RNA flaviviruses. This study aims to investigate the role of TLR7 in inducing adaptive immune response in mice against JEV.In vitroandin vivostudies were conducted to examine the expression of toll-like receptors (TLRs) in mice. After JEV infection, physical parameters of mice (survival rate and body weight) were evaluated, and organs or cells were collected for further analysis. The expression of TLR7 was increased significantly as compare to other TLR molecules post-JEV infection. The expression of CD80, CD86, and CD273 on bone marrow-derived dendritic cells was increased significantly in TLR7-/-mice. Furthermore, viral load was also increased significantly in TLR7-/-mice as compare to C57BL/6 mice. But there was no significant difference among survival rate and body weight in TLR7-/-mice as compare to C57BL/6. Interestingly, we also found that TLR8 was upregulated in TLR7-/-mice. The study concluded that TLR8 was upregulated in TLR7-deficient mice, and it might play a compensatory role in the immune response in TLR7-/-mice.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Awais
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Xianwu
authors.initials: X
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Kunlun
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: Zhen F
authors.initials: ZF
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
authors.forename: Min
authors.initials: M
authors.lastname: Cui
keywords: Japanese encephalitis virus; TLR7; TLR8; immune response; viral load