title: Infundibular protein and RNA microarray analyses from affected and clinically non-affected scalp in male androgenetic alopecia patients.
abstract: Non-invasive sample collection methods could facilitate clinical research on hair diseases. In an exploratory experimental study on six male volunteers with untreated androgenetic alopecia (AGA), Hamilton-Norwood stage IIIv-IV, skin surface and infundibular protein as well as RNA extracts from plucked hair follicles were analyzed from frontal skin, vertex and clinically unaffected occiput. Slightly increased levels of inflammatory markers were only found in AGA-affected scalp skin and infundibulum, not in RNA from plucked hair follicles. RNA expression profiles point towards differential expression of genes involved in hair cycle regulation, hair keratin production, but also RNA methylation and ion channel regulation.
authors.affiliation: Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Annika
authors.identifier: 0000-0001-9134-8413
authors.initials: A
authors.lastname: Vogt
authors.affiliation: Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Eva K B
authors.initials: EKB
authors.lastname: Pfannes
authors.affiliation: Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Fimmel
authors.affiliation: Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Hadam
authors.affiliation: Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Andruck
authors.affiliation: Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Kottner
authors.affiliation: Clinical Research Center for Hair and Skin Science, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Blume-Peytavi
mesh_terms: D000505:Alopecia / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D019175:DNA Methylation; D018450:Disease Progression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006197:Hair / Q000378:metabolism*; D018859:Hair Follicle / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007249:Inflammation; D007633:Keratins / Q000378:metabolism; D008297:Male; D008745:Methylation; D010865:Pilot Projects; D012313:RNA / Q000378:metabolism; D012535:Scalp / Q000378:metabolism*; D012867:Skin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D012313:RNA; D007633:Keratins
keywords: androgenetic alopecia; cyanoacrylate skin surface stripping; gene expression; hair follicle; inflammation