title: Mass spectrometry-guided isolation of two new dihydrobenzophenanthridine alkaloids from Macleaya cordata.
abstract: Two new alkaloids 6-hydroxyethyldihydrochelerythrine (1) and 6-hydroxymethyl-7,8-demethylenedihydrochelerythrine (2) together with two analogues named maclekarpine E (3) and 6-hydroxymethyldihydrosanguinarine (4) were detected primarily from the leaves of Macleaya cordata by their characteristic mass spectrometry (MS) and tandem mass spectrometry (MS/MS). And then isolation of four targeted-compounds was performed by column chromatography and preparation HPLC under the guiding of MS. Finally, their structures were determined by spectrum analysis. Alkaloids 3 and 4 were isolated from this plant medicine for the first time.
authors.affiliation: a College of Pharmacy , Hunan University of Chinese Medicine , Changsha , China.
authors.forename: Zhi-Xing
authors.initials: ZX
authors.lastname: Qing
authors.affiliation: a College of Pharmacy , Hunan University of Chinese Medicine , Changsha , China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.affiliation: d Pharmacy of Traditional Medicine , Tongde Hospital of Zhejiang Province , Zhejiang , China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yu
authors.affiliation: b National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resoures and Initiative , Hunan Agricultural University , Changsha , China.|c Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients , Hunan Agricultural University , Changsha , China.
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Yang
authors.affiliation: b National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resoures and Initiative , Hunan Agricultural University , Changsha , China.|c Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients , Hunan Agricultural University , Changsha , China.
authors.forename: Jing-Hong
authors.initials: JH
authors.lastname: Liu
authors.affiliation: a College of Pharmacy , Hunan University of Chinese Medicine , Changsha , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xiang
authors.affiliation: b National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resoures and Initiative , Hunan Agricultural University , Changsha , China.|c Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients , Hunan Agricultural University , Changsha , China.
authors.forename: Hua-Liang
authors.initials: HL
authors.lastname: Cao
authors.affiliation: b National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resoures and Initiative , Hunan Agricultural University , Changsha , China.|c Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients , Hunan Agricultural University , Changsha , China.
authors.forename: Pi
authors.initials: P
authors.lastname: Cheng
authors.affiliation: a College of Pharmacy , Hunan University of Chinese Medicine , Changsha , China.|b National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resoures and Initiative , Hunan Agricultural University , Changsha , China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zeng
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015394:Molecular Structure; D028101:Papaveraceae / Q000737:chemistry*; D013057:Spectrum Analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids
keywords: 6-hydroxyethyldihydrochelerythrine; 6-hydroxymethyl-7,8-demethylenedihydro-chelerythrine; Macleaya cordata; dihydrobenzophenanthridine alkaloids; mass spectrometry-guided isolation