title: A Novel Impedimetric Microfluidic Analysis System for Transgenic Protein Cry1Ab Detection.
abstract: Impedimetric analysis method is an important tool for food safety detection. In this work, a novel impedimetric microfluidic analysis system consisted of a printed gold electrode chip and a microfluidic flow cell was developed for sensitive and selective detection of transgenic protein Cry1Ab. Anti-Cry1Ab aptamer coated magnetic beads were used to recognize transgenic protein Cry1Ab and form Cry1Ab-aptamer modified magnetic beads. After separation, the obtained Cry1Ab-aptamer modified magnetic beads were dissolved in 0.01 M mannitol and followed by injection into the microfluidic flow cell for impedimetric measurement. At the frequency of 358.3 Hz, the impedance signal shows a good linearity with the concentrations of Cry1Ab protein at a range from 0 to 0.2 nM, and the detection limit is 0.015 nM. The results demonstrate that the impedimetric microfluidic analysis system provides an alternative way to enable sensitive, rapid and specific detection of transgenic protein Cry1Ab.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Shunru
authors.initials: S
authors.lastname: Jin
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Zunzhong
authors.initials: Z
authors.lastname: Ye
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yixian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Ying
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis*; D017097:Electric Impedance; D004731:Endotoxins / Q000032:analysis*; D006460:Hemolysin Proteins / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis