title: DNA supercoiling during transcription.
abstract: The twin-supercoiled-domain model describes how transcription can drive DNA supercoiling, and how DNA supercoiling, in turn plays an important role in regulating gene transcription.In vivoandin vitroexperiments have disclosed many details of the complex interactions in this relationship, and recently new insights have been gained with the help of genome-wide DNA supercoiling mapping techniques and single molecule methods. This review summarizes the general mechanisms of the interplay between DNA supercoiling and transcription, considers the biological implications, and focuses on recent important discoveries and technical advances in this field. We highlight the significant impact of DNA supercoiling in transcription, but also more broadly in all processes operating on DNA.
authors.affiliation: School of Physics ; State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou, 510275, PRC.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Physics - Laboratory of Atomic and Solid State Physics ; Howard Hughes Medical Institute, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Wang
keywords: chromatin; gene regulation; mechanics; supercoiling; torque; transcription