title: Chemical composition and phagocyte immunomodulatory activity ofFerula iliensisessential oils.
abstract: Essential oil extracts fromFerula iliensishave been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were (E)-propenylsec-butyl disulfide (15.7-39.4%) and (Z)-propenylsec-butyl disulfide (23.4-45.0%).Ferulaessential oils stimulated [Ca2+]imobilization in human neutrophils and activated ROS production in human neutrophils and murine bone marrow phagocytes. Activation of human neutrophil [Ca2+]iflux byFerulaessential oils was dose-dependently inhibited by capsazepine, a TRPV1 channel antagonist, indicating that TRPV1 channels mediate this response. Furthermore,Ferulaessential oils stimulated Ca2+influx in TRPV1 channel-transfected HEK293 cells and desensitized the capsaicin-induced response in these cells. Additional molecular modeling with known TRPV1 channel agonists suggested that the active component is likely to be (Z)-propenylsec-butyl disulfide. Our results provide a cellular and molecular basis to explain at least part of the beneficial therapeutic properties of FEOs.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey.
authors.forename: Gulmira
authors.initials: G
authors.lastname: Özek
authors.affiliation: Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Schepetkin
authors.affiliation: Institute of Plant Biology and Biotechnology, Almaty, Republic of Kazakhstan.|Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Republic of Kazakhstan.
authors.forename: Gulzhakhan A
authors.initials: GA
authors.lastname: Utegenova
authors.affiliation: Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA.
authors.forename: Liliya N
authors.initials: LN
authors.lastname: Kirpotina
authors.affiliation: Department of Biological Sciences, Kent State University, Kent, Ohio, USA.
authors.forename: Spencer R
authors.initials: SR
authors.lastname: Andrei
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey.
authors.forename: Temel
authors.initials: T
authors.lastname: Özek
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Near East University, Nicosia, North Cyprus.
authors.forename: Kemal Hüsnü Can
authors.initials: KHC
authors.lastname: Başer
authors.affiliation: Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Republic of Kazakhstan.
authors.forename: Karime T
authors.initials: KT
authors.lastname: Abidkulova
authors.affiliation: Institute of Plant Biology and Biotechnology, Almaty, Republic of Kazakhstan.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Kushnarenko
authors.affiliation: Department of Biotechnology and Organic Chemistry, Tomsk Polytechnic University, Tomsk, Russia; and.|Department of Chemistry, Altai State Technical University, Barnaul, Russia.
authors.forename: Andrei I
authors.initials: AI
authors.lastname: Khlebnikov
authors.affiliation: Department of Biological Sciences, Kent State University, Kent, Ohio, USA.
authors.forename: Derek S
authors.initials: DS
authors.lastname: Damron
authors.affiliation: Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA; mquinn@montana.edu.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Quinn
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D005309:Ferula / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D050916:TRPV Cation Channels / Q000378:metabolism
chemical_list: D009822:Oils, Volatile; D050916:TRPV Cation Channels; D002118:Calcium
keywords: calcium flux; molecular modeling; neutrophil; reactive oxygen species; transient receptor potential vanilloid 1 channel