title: Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus.
abstract: The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus.
  Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method.
  A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls.
  Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings.
authors.affiliation: Shenzhen Nanshan Center for Chronic Disease Control, Department of Chronic Disease Prevention and Control, Shenzhen, 518054, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Ma
authors.affiliation: Ningbo Medical Center Lihuili Eastern Hospital, Department of Endocrinology, Ningbo 315040, China.
authors.forename: Jufang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Ningbo University, School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shenzhen Nanshan Center for Chronic Disease Control, Department of Chronic Disease Prevention and Control, Shenzhen, 518054, China.
authors.forename: Changyi
authors.initials: C
authors.lastname: Wang
authors.affiliation: Ningbo University, School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Duan
authors.affiliation: Ningbo University, School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China.
authors.forename: Nanjia
authors.initials: N
authors.lastname: Lu
authors.affiliation: Ningbo University, School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China.
authors.forename: Kaiyue
authors.initials: K
authors.lastname: Wang
authors.affiliation: Ningbo University, School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Ningbo University, School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China.
authors.forename: Kaibo
authors.initials: K
authors.lastname: Gu
authors.affiliation: Shenzhen Nanshan Center for Chronic Disease Control, Department of Chronic Disease Prevention and Control, Shenzhen, 518054, China.
authors.forename: Sihan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Shenzhen Nanshan Center for Chronic Disease Control, Department of Chronic Disease Prevention and Control, Shenzhen, 518054, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Kunming Medical University, Department of Science and Technology, Kunming, 650500, China.
authors.forename: Dingyun
authors.initials: D
authors.lastname: You
authors.affiliation: Ningbo University, School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Han
mesh_terms: D000368:Aged; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000097:blood / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs