title: Heterogeneity of mast cells and expression of Annexin A1 protein in a second degree burn model with silver sulfadiazine treatment.
abstract: Mast cells (MCs) participate in all stages of skin healing and one of their mediators is the Annexin A1 protein (AnxA1), linked to inflammation, proliferation, migration and apoptosis processes, but not studied in thermal burns yet. Therefore, our objectives were to evaluate the behavior of MCs and AnxA1 in a second degree burn model, treated or not with silver sulfadiazine 1% (SDP 1%) and associated to macrophages quantification and cytokines dosages. MCs counts showed few cells in the early stages of repair but increased MCs in the final phases in the untreated group. The normal skin presented numerous tryptase-positive MCs that were reduced after burning in all analyzed periods. Differently, few chymase-positive MCs were observed in the early stages of healing, however, increased chymase-positive MCs were found at the final phase in the untreated group. MCs also showed high immunoreactivity for AnxA1 on day 3 in both groups. In the tissue there was a strong protein expression in the early stages of healing, but in the final phases only in the SDP treated animals. TNF-α, IL-1β, IL-6, IL-10 and MCP-1 levels and macrophages quantification were increased in inflammation and reepithelialization phases. Reduced IL-1β, IL-6 and IL-10 levels and numerous macrophages occurred in the treated animals during tissue repair. Our results indicate modulation in the profile of MCs and AnxA1expression during healing by the treatment with SDP 1%, pointing them as targets for therapeutic interventions on skin burns.
authors.affiliation: Integrated College Padre Albino Foundation (FIPA), Catanduva, São Paulo, Brazil.|Department of Biology, Laboratory of Immunomorphology, São Paulo State University, (UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Helena Ribeiro
authors.initials: HR
authors.lastname: Souza
authors.affiliation: Department of Biology, Laboratory of Immunomorphology, São Paulo State University, (UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Lucas Ribeiro
authors.initials: LR
authors.lastname: de Azevedo
authors.affiliation: Department of Biology, Laboratory of Immunomorphology, São Paulo State University, (UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Lucas
authors.initials: L
authors.lastname: Possebon
authors.affiliation: Department of Biology, Laboratory of Immunomorphology, São Paulo State University, (UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Sara de Souza
authors.initials: SS
authors.lastname: Costa
authors.affiliation: Integrated College Padre Albino Foundation (FIPA), Catanduva, São Paulo, Brazil.
authors.forename: Melina Mizusaki
authors.initials: MM
authors.lastname: Iyomasa-Pilon
authors.affiliation: Department of Biology, Laboratory of Immunomorphology, São Paulo State University, (UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Sonia Maria
authors.initials: SM
authors.lastname: Oliani
authors.affiliation: Integrated College Padre Albino Foundation (FIPA), Catanduva, São Paulo, Brazil.|Department of Biology, Laboratory of Immunomorphology, São Paulo State University, (UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Girol
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000378:metabolism*; D002056:Burns / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D020405:Dermis / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D004817:Epidermis / Q000187:drug effects / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006632:Histamine / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012837:Silver Sulfadiazine / Q000494:pharmacology* / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects
chemical_list: D017305:Annexin A1; D016207:Cytokines; D006632:Histamine; D012837:Silver Sulfadiazine