title: Altered intestinal microbiota in patients with chronic pancreatitis: implications in diabetes and metabolic abnormalities.
abstract: Intestinal dysbiosis and its functional implications in chronic pancreatitis (CP) have not been elaborately studied. We evaluated the taxonomic and functional alterations in intestinal microbiota in 30 well-characterised patients with CP (16 without, 14 with diabetes) and 10 healthy controls. The patients with CP and diabetes had significantly longer disease duration and greater degree of malnutrition. There was increase in plasma endotoxin concentrations from controls to CP non-diabetics to CP diabetics. We observed significant differences in richness and alpha diversity between the groups. We also observed increase in the Firmicutes:Bacteroidetes ratio in CP patients without and with diabetes. There was reduction in abundance of Faecalibacterium prausnitzii and Ruminococcus bromii from controls to CP non-diabetics to CP diabetics. On the other hand, there was increase in LPS (endotoxin) synthetic pathways (KEGG orthology) in the groups. Faecalibacterium prausnitzii abundance correlated negatively with plasma endotoxin and glycemic status; while plasma endotoxin correlated positively with blood glucose and negatively with plasma insulin. Our results have important implications for future studies exploring mechanistic insights on secondary diabetes in CP.
authors.affiliation: Division of Basic Sciences, Asian Healthcare Foundation, Hyderabad, India.
authors.forename: Sai Manasa
authors.initials: SM
authors.lastname: Jandhyala
authors.affiliation: Dept. of Clinical Nutrition, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: A
authors.initials: A
authors.lastname: Madhulika
authors.affiliation: Dept. of Biochemistry, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: G
authors.initials: G
authors.lastname: Deepika
authors.affiliation: Dept. of Surgical Gastroenterology, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: G Venkat
authors.initials: GV
authors.lastname: Rao
authors.affiliation: Dept. of Medical Gastroenterology, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: D Nageshwar
authors.initials: DN
authors.lastname: Reddy
authors.affiliation: Division of Basic Sciences, Asian Healthcare Foundation, Hyderabad, India.
authors.forename: Chivukula
authors.initials: C
authors.lastname: Subramanyam
authors.affiliation: Division of Basic Sciences, Asian Healthcare Foundation, Hyderabad, India.
authors.forename: Mitnala
authors.initials: M
authors.lastname: Sasikala
authors.affiliation: Division of Basic Sciences, Asian Healthcare Foundation, Hyderabad, India.|Dept. of Medical Gastroenterology, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Rupjyoti
authors.initials: R
authors.lastname: Talukdar
mesh_terms: D000328:Adult; D044822:Biodiversity; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis / Q000209:etiology*; D018450:Disease Progression; D064806:Dysbiosis; D004734:Energy Metabolism; D005260:Female; D000069196:Gastrointestinal Microbiome; D006801:Humans; D008297:Male; D008659:Metabolic Diseases / Q000175:diagnosis / Q000209:etiology*; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D008875:Middle Aged; D050500:Pancreatitis, Chronic / Q000150:complications* / Q000175:diagnosis