title: Viability-reducing activity of Coryllus avellana L. extracts against human cancer cell lines.
abstract: The increasing rate of cancer incidence has encouraged the search for novel natural sources of anticancer compounds. The presence of small quantities of taxol and taxanes in Corylus avellana L. has impelled new potential applications for this plant in the field of biomedicine. In the present work, the cell viability-reducing activity of stems and leaves from three different hazel trees was studiedagainst three human-derived cancer cell lines (HeLa, HepG2 and MCF-7). Both leaf and stem extracts significantly reduced viability of the three cell lines either after maceration with methanol or using taxane extraction methods. Since maceration reduced cell viability to a greater extent than taxane extraction methods, we scaled up the maceration extraction process using a method for solid/liquid extraction (Zippertex technology). Methanol leaf extracts promoted a higher reduction in viability of all cell lines assayed than stem extracts. Fractionation of methanol leaf extracts using silica gel chormatography led to the purification and identification of two compounds by HPLC-MS and NMR: (3R,5R)-3,5-dihydroxy-1,7-bis(4-hydroxyphenyl) heptane 3-O-β-d-glucopyranoside and quercetin-3-O-rhamnoside. The isolated compounds decreased viability of HeLa and HepG2 cells to a greater extent than MCF-7 cells. Our results suggest a potential use of C. avellana extracts in the pharmacotherapy of cervical cancer and hepatocarcinoma and, to a lesser extent, breast cancer.
authors.affiliation: Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Avda. Dr. Aiguader 80, E-08003, Barcelona, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Gallego
authors.affiliation: Departament de Bioquímica i Fisiologia, Secció de Bioquímica i Biologia Molecular, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Avda. Joan XXIII 27-31, E-08028 Barcelona, Spain.
authors.forename: Isidoro
authors.initials: I
authors.lastname: Metón
authors.affiliation: Departament de Bioquímica i Fisiologia, Secció de Bioquímica i Biologia Molecular, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Avda. Joan XXIII 27-31, E-08028 Barcelona, Spain.
authors.forename: Isabel V
authors.initials: IV
authors.lastname: Baanante
authors.affiliation: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique, CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette cedex, France.
authors.forename: Jamal
authors.initials: J
authors.lastname: Ouazzani
authors.affiliation: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique, CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette cedex, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Adelin
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Avda. Joan XXIII 27-31, E-08028 Barcelona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Palazon
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Avda. Joan XXIII 27-31, E-08028 Barcelona, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Bonfill
authors.affiliation: Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Avda. Dr. Aiguader 80, E-08003, Barcelona, Spain. Electronic address: elisabeth.moyano@upf.edu.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Moyano
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D031211:Corylus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D000432:Methanol; D017239:Paclitaxel / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012997:Solvents
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012997:Solvents; D017239:Paclitaxel; D000432:Methanol
keywords: Anticancer activity; Corylus avellana; Human cancer cells; Plant extracts; Taxol