title: Characterization of the CA1 pyramidal neurons in rat model of hepatic cirrhosis: insights into their electrophysiological properties.
abstract: Although the key contributors of altering neurological function in hepatic encephalopathy are relatively well known, the electrophysiological mechanism of CA1 damage, a key vulnerable area during hyperammonemia, have not yet been defined. Therefore, here we focus on the electrophysiological mechanisms of cognitive impairments following bile duct ligation (BDL). We performed patch-clamp recordings from the CA1 pyramidal neurons in hippocampus of male Wistar rats, which underwent sham or BDL surgery. A striking electrophysiological change of hippocampal neurons in experimental model of BDL was observed in the present study. Spontaneous firing frequency and rate of action potential (AP) rebound was decreased and afterhyperpolarization amplitude (AHP) was increased significantly in hippocampal cells of BDL animals compared to sham group. Together, the results suggest that altered intrinsic properties of the hippocampal neurons may contribute to the cognitive abnormalities during hepatic encephalopathy (HE), highlighting the electrophysiological mechanisms for providing new treatments against HE.
authors.affiliation: Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, 76198-13159, Iran.
authors.forename: Mahshid
authors.initials: M
authors.lastname: Tahamtan
authors.affiliation: Social Determinants of Health Research Center, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Aghaei
authors.affiliation: Biochemical Department, Jiroft University of Medical Sciences, Kerman, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Pooladvand
authors.affiliation: Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, 76198-13159, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Sheibani
authors.affiliation: Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Khaksari
authors.affiliation: Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, 76198-13159, Iran. shabanimoh@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shabani
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000503:physiopathology*; D004195:Disease Models, Animal; D055724:Electrophysiological Phenomena / Q000502:physiology*; D008103:Liver Cirrhosis / Q000503:physiopathology*; D008297:Male; D009924:Organ Culture Techniques; D017966:Pyramidal Cells / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
keywords: Bile duct ligation; Electrophysiology; Firing properties; Hepatic cirrhosis