title: Isolation and characterization of bioactive polyacetylenes Panax ginseng Meyer roots.
abstract: Panax ginseng has been studied for its chemo-preventive properties and pharmaceutical potential. Polyacetylenic compounds isolated from Panax ginseng root typically comprised of non-polar C17compound have been reported to exhibit bioactive properties. The objective of this project is to extract, isolate, and characterize bioactive polyacetylenes from Panax ginseng root using various extraction and separation methods Ginseng was extracted by reflux using methanol, ethanol, hexane, ethyl acetate, methanolic ultrasonication. The extracts were partitioned with hexane to obtain water-soluble portion and hexane-soluble portion. Hexane was subsequently removed under vacuum, and formed a crude polyacetylenes extract (crude PA). Silica gel chromatography and semi-preparative HPLC were utilized to prepare 5 fractions and the polyacetylenes were measure by HPLC and molecular weights confirm my APCI-MS and MNR. The bioactive effect was measured by MTT viability assay using murine 3T3-L1 cells. Extraction with methanol under reflux produced significantly larger amount of polyacetylenes (p<0.05). Liquid-liquid extraction and column chromatography were used to separate polyacetylenic compounds into five different fractions. Major polyacetylenes, panaxynol and panaxydol were found in fraction 1 and 2 respectively. Dose-response relationships were observed in 3T3-L1 cells and LC50 were 13.52±3.05μg/mL (fraction 1), 3.69±1.09μg/mL (fraction 2), 52.88±11.16μg/mL (fraction 3), 85.91±27.37μg/mL (fraction 4) and 135.52±32.91μg/mL (fraction 5). Fraction 2 containing panaxydol was found to have exhibited the greatest anti-proliferative effects on 3T3-L1 preadipocytes. Extraction with methanol under reflux produced significantly more polyacetylenes. Fractions that contain panaxydol was the most cytotoxic.
authors.affiliation: National University Hospital, Singapore; National University of Singapore, Singapore.
authors.forename: Chia-Rou
authors.initials: CR
authors.lastname: Yeo
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore.
authors.forename: Jin-Jie
authors.initials: JJ
authors.lastname: Yong
authors.affiliation: Private Bag 11222, Massey Institute of Food Science and Technology, School of Food and Nutrition, Massey University, Palmerston North, 4442, New Zealand. Electronic address: D.G.Popovich@massey.ac.nz.
authors.forename: David G
authors.initials: DG
authors.lastname: Popovich
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods; D051379:Mice; D005894:Panax; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018517:Plant Roots; D053279:Polyynes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D053279:Polyynes
keywords: 3T3-L1; Cytotoxicity; Panax ginseng; Panaxydol; Panaxynol; Polyacetylenes