title: Effects of silk fibroin in murine dry eye.
abstract: The study aimed to investigate the effects of silk fibroin in a mouse model of dry eye. The experimental dry eye mouse model was developed using more than twelve-weeks-old NOD.B10.H2bmice exposing them to 30-40% ambient humidity and injecting them with scopolamine hydrobromide for 10 days. Tear production and corneal irregularity score were measured by the instillation of phosphate buffered saline or silk fibroin. Corneal detachment and conjunctival goblet cell density were observed by hematoxylin and eosin or periodic acid Schiff staining in the cornea or conjunctiva. The expression of inflammatory markers was detected by immunohistochemistry in the lacrimal gland. The silk group tear production was increased, and corneal smoothness was improved. The corneal epithelial cells and conjunctival goblet cells were recovered in the silk groups. The expression of inflammatory factors was inhibited in the lacrimal gland of the silk group. These results show that silk fibroin improved the cornea, conjunctiva, and lacrimal gland in the mouse model of dry eye. These findings suggest that silk fibroin has anti-inflammatory effects in the experimental models of dry eye.
authors.affiliation: Ocular Neovascular Disease Research Center and T2B infrastructure Center for Ocular Disease, Inje University Busan Paik Hospital, Busan 614-735, Korea.|Department of Ophthalmology, Inje University College of Medicine, Inje University Busan Paik Hospital, Busan 614-735, Korea.
authors.forename: Chae Eun
authors.initials: CE
authors.lastname: Kim
authors.affiliation: Ocular Neovascular Disease Research Center and T2B infrastructure Center for Ocular Disease, Inje University Busan Paik Hospital, Busan 614-735, Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Yeung Kyu
authors.initials: YK
authors.lastname: Yeon
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, Korea.|Departments of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
authors.affiliation: Ocular Neovascular Disease Research Center and T2B infrastructure Center for Ocular Disease, Inje University Busan Paik Hospital, Busan 614-735, Korea.|Department of Ophthalmology, Inje University College of Medicine, Inje University Busan Paik Hospital, Busan 614-735, Korea.
authors.forename: JaeWook
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D003228:Conjunctiva / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D003315:Cornea / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D015352:Dry Eye Syndromes / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D005349:Fibroins / Q000494:pharmacology*; D015870:Gene Expression; D020397:Goblet Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D007765:Lacrimal Apparatus / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000276:immunology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology; D051379:Mice; D016688:Mice, Inbred NOD; D009883:Ophthalmic Solutions / Q000494:pharmacology*; D012601:Scopolamine; D013666:Tears / Q000187:drug effects* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D009883:Ophthalmic Solutions; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D005349:Fibroins; D012601:Scopolamine; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse