title: Kinetic and molecular analyses reveal isoprene degradation potential of Methylobacterium sp.
abstract: Efforts were made to isolate and characterize bacteria capable of growing on methane and organic compounds, and to achieve the simultaneous degradation of more than one pollutant. Among the methanotrophs, species of Methylobacterium was able to catabolize a variety of hydrocarbons, including the branched-chain alkenes. Therefore, laboratory incubations experiments were carried out in batch mode to assess the potential of Methylobacterium sp. PV1 for degrading isoprene, the low-molecular-weight alkene, the most abundant non-methane volatile hydrocarbon present in the environment. Methylobacterium sp. PV1, isolated from paddy field soil, was characterized by pmoA and 16S rRNA gene sequencing and FAME analysis, and used for isoprene degradation. The kinetics of biodegradation is studied using the Michaelis-Menten model. The optimum degradation (80%) with maximum average relative degradation rate was observed at 150ppm isoprene. The degradation products were also analyzed using FTIR.
authors.affiliation: Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Navnita
authors.initials: N
authors.lastname: Srivastva
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Bangalore 560056, India.
authors.forename: P
authors.initials: P
authors.lastname: Vishwakarma
authors.affiliation: Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Y
authors.initials: Y
authors.lastname: Bhardwaj
authors.affiliation: Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: A
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Bangalore 560056, India.
authors.forename: K
authors.initials: K
authors.lastname: Manjunath
authors.affiliation: Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: skdubey@bhu.ac.in.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Dubey
mesh_terms: D001673:Biodegradation, Environmental; D002070:Butadienes / Q000378:metabolism*; D045782:Hemiterpenes / Q000378:metabolism*; D020580:Methylobacterium; D010420:Pentanes / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D012336:RNA, Ribosomal, 16S; C005059:isoprene
keywords: Biodegradation; FTIR; Isoprene; Kinetics; Methylobacterium