title: MiR-30b-5p acts as a tumor suppressor, repressing cell proliferation and cell cycle in human hepatocellular carcinoma.
abstract: MicroRNAs have been verified to participate in various biological behaviors of different tumors, via multiple signaling pathways. Many kinds of microRNAs in hepatocellular carcinoma have been researched. However, miR-30b-5p hasn't been included. Our study aim at the impacts of miR-30b-5p on HCC and the pathway it mediating. The results showed miR-30b-5p was significant downregulated in HCC tissues and cell lines. With clinical data, we've discovered miR-30b-5p was correlated with several clinical pathological characteristics, such as survival time, tumor size, HBV infected, pathological stage, differentiation and intrahepatic metastasis. Also we illustrated miR-30b-5p repressed cell proliferation and cell cycle of HCC cell lines. For a further study, we figured out that miR-30b-5p mediated DNMT3A to repress proliferation, meanwhile it targeted USP37 for decelerating cell cycle. This discovery inferred miR-30b-5p a potential favorable biomarker and therapeutic target for HCC diagnosis and treatment.
authors.affiliation: Department of Hepatobiliary Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China. Electronic address: liuzs53@sina.com.
authors.forename: Zhisu
authors.initials: Z
authors.lastname: Liu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D000090205:DNA Methyltransferase 3A; D015536:Down-Regulation; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: C000717967:DNMT3A protein, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D010450:Endopeptidases; C559980:USP37 protein, human
keywords: Cell cycle; DNMT3A; Hepatocellular carcinoma; Proliferation; USP37; miR-30b-5p