title: The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation.
abstract: Microtubules tether centrosomes together during interphase. How this is accomplished and what benefit it provides to the cell is not known. We have identified a bipolar, minus-end-directed kinesin, Kif25, that suppresses centrosome separation. Kif25 is required to prevent premature centrosome separation during interphase. We show that premature centrosome separation leads to microtubule-dependent nuclear translocation, culminating in eccentric nuclear positioning that disrupts the cortical spindle positioning machinery. The activity of Kif25 during interphase is required to maintain a centred nucleus to ensure the spindle is stably oriented at the onset of mitosis.
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Decarreau
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Wagenbach
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Lynch
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Halpern
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA.|Department of Chemistry, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Vaughan
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Kollman
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Linda
authors.initials: L
authors.lastname: Wordeman
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000737:chemistry* / Q000378:metabolism*; D018374:LLC-PK1 Cells; D008938:Mitosis; D055503:Protein Multimerization; D008941:Spindle Apparatus / Q000378:metabolism*; D013552:Swine
chemical_list: C117477:KIF25 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D016547:Kinesins