title: MYB76 Inhibits Seed Fatty Acid Accumulation in Arabidopsis.
abstract: The MYB family of transcription factors is important in regulatory networks controlling development, metabolism and responses to biotic and abiotic stresses in Arabidopsis. However, their role in regulating fatty acid accumulation in seeds is still largely unclear. Here, we found that MYB76, localized in the nucleus, was predominantly expressed in developing seeds during maturation. Themyb76mutation caused a significant increase in the amounts of total fatty acids and several major fatty acid compositions in mature seeds, suggesting that MYB76 functioned as an important repressor during seed oil biosynthesis. RNA sequencing and quantitative real-time PCR analysis revealed remarkable alteration of numerous genes involved in photosynthesis, fatty acid biosynthesis, modification, and degradation, and oil body formation inmyb76seeds at 12 days after pollination. These results help us to understand the novel function of MYB76 and provide new insights into the regulatory network of MYB transcriptional factors controlling seed oil accumulation in Arabidopsis.
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Duan
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Jin
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Chenhao
authors.initials: C
authors.lastname: Gao
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Shuanghui
authors.initials: S
authors.lastname: Qi
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Kaige
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Hai
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Haoli
authors.initials: H
authors.lastname: Ma
authors.affiliation: College of Agronomy, Northwest A&F University Yangling, China.
authors.forename: Mingxun
authors.initials: M
authors.lastname: Chen
keywords: Arabidopsis; MYB76; RNA sequencing; fatty acids; seeds