title: The correlation between osteopontin adsorption and cell adhesion to mixed self-assembled monolayers of varying charges and wettability.
abstract: Osteopontin (OPN) is a key mediator of cell interactions with biomaterials. However, few studies have been dedicated to studying cell adhesion on OPN-adsorbed substrates with controlled charge and wettability. Here, amino-carboxyl (NH2/COOH) and hydroxyl-methyl (OH/CH3) mixed self-assembled monolayers (SAMs) of varying charges and wettability, respectively, were used as controllable model surfaces to study OPN adsorption and subsequent mesenchymal stem cell (MSC) adhesion. The amount of OPN adsorbed onto the NH2/COOH mixed SAMs appeared to monotonically depend on the surface charge, whereas only a moderately hydrophilic surface was conducive to OPN adsorption on OH/CH3mixed SAMs. The results correlated well with cell spreading on OPN-coated surfaces in a serum-free medium culture. In addition, the OH/CH3mixed SAMs with moderate wettability tended to promote β1, β3, αvand α5integrins, indicating that wettability may guide cell adhesion by mediating the integrins signaling pathway. This work will have reference value for designing biologically responsive substrate surfaces.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China. imwangyj@163.com and National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Lijing
authors.identifier: http://orcid.org/0000-0003-2710-5112
authors.initials: L
authors.lastname: Hao
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China. imwangyj@163.com and National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Tianjie
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China. imwangyj@163.com and National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China. imwangyj@163.com and National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Naru
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Fuzhai
authors.initials: F
authors.lastname: Cui
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China. imwangyj@163.com and National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Shi
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China. imwangyj@163.com and National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Chang
authors.identifier: http://orcid.org/0000-0002-6460-0764
authors.initials: C
authors.lastname: Du
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China. imwangyj@163.com and National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
authors.forename: Yingjun
authors.identifier: http://orcid.org/0000-0002-6332-9706
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000586:Amination; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008745:Methylation; D051379:Mice; D053495:Osteopontin / Q000737:chemistry*; D013499:Surface Properties; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D053495:Osteopontin