title: Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection.
abstract: Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber secretion. Inhibition of intracellular calcium [Ca2+]isignaling caused diminished fiber secretion, which was associated with diminished progeny production. Thus, fiber and penton base are actively and separately secreted during the early stages of Ad2 or Ad5 infection, their secretion may play important role in Ad life cycle.
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Hou
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Lin
authors.affiliation: The Rausing Laboratory, Department of Neurosurgery, Lund University, Lund, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Rebetz
authors.affiliation: Viral Infections & Comparative Pathology, UMR-754 UCBL-INRA-EPHE, Université Lyon 1, Lyon Cedex 07, France.
authors.forename: Saw-See
authors.initials: SS
authors.lastname: Hong
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing, China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ran
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing, China. Electronic address: XFan@bnu.edu.cn.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Fan
mesh_terms: D000072283:A549 Cells; D008841:Actin Cytoskeleton / Q000378:metabolism; D000258:Adenovirus Infections, Human / Q000473:pathology*; D000260:Adenoviruses, Human / Q000145:classification / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D005434:Flow Cytometry; D057809:HEK293 Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008870:Microtubules / Q000378:metabolism; D024462:Myosin Type II / Q000037:antagonists & inhibitors; D011991:Receptors, Virus / Q000378:metabolism; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors
chemical_list: D036022:Capsid Proteins; D011991:Receptors, Virus; C018315:hexon capsid protein, Adenovirus; C043322:penton protein, adenovirus; D054460:rho-Associated Kinases; D024462:Myosin Type II
keywords: Adenovirus; Fiber protein; Penton base; Secretion