title: Dual-Laboratory Validation of a Method for the Determination of Fructans in Infant Formula and Adult Nutritionals: First Action 2016.14.
abstract: Until recently, only two AOAC Official MethodsSM have been available for the analysis of fructans: Method 997.08 and Method 999.03. Both are based on the analysis of the fructan component monosaccharides (glucose and fructose) after hydrolysis. The two methods have some limitations due to the strategies used for removing background interferences (such as from sucrose, α-glucooligosaccharides, and free sugars). The method described in this paper has been developed to overcome those limitations. The method is largely based on Method 999.03 and uses combined enzymatic and SPE steps to remove the interfering components without impacting the final analytical result. The method has been validated in two laboratories on infant formula and adult nutritionals. Recoveries were in the range of 86-119%, with most being in the range of 91-104%. RSDr values were in the range of 0.7-2.6%, with one exception when the fructan concentration was close to the LOQ, resulting in an RSDr of 8.9%. The performance is generally within the requirements outlined in the AOAC Standard Method Performance Requirements (SMPR® 2014.002), which specifies recoveries in the range of 90-110% and RSDr values below 6%.
authors.affiliation: Rotating Disc BV, 9753 HV Haren, The Netherlands.
authors.forename: Kommer
authors.initials: K
authors.lastname: Brunt
authors.affiliation: Eurofins Carbohydrate Competence Centre, 8440 AT Heerenveen, The Netherlands.
authors.forename: Peter
authors.initials: P
authors.lastname: Sanders
authors.affiliation: Nestlé Research Centre, 1000 Lausanne, Switzerland.
authors.forename: Véronique
authors.initials: V
authors.lastname: Spichtig
authors.affiliation: Eurofins Carbohydrate Competence Centre, 8440 AT Heerenveen, The Netherlands.
authors.forename: Veronica
authors.initials: V
authors.lastname: Ernste-Nota
authors.affiliation: Nestlé Research Centre, 1000 Lausanne, Switzerland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Sawicka
authors.affiliation: Nestlé Quality Assurance Centre, York, United Kingdom.
authors.forename: Kimberley
authors.initials: K
authors.lastname: Iwanoff
authors.affiliation: Eurofins Carbohydrate Competence Centre, 8440 AT Heerenveen, The Netherlands.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Van Soest
authors.affiliation: Robert Gordon University, Aberdeen, AB10 7AQ, United Kingdom.
authors.forename: Paul Kong Thoo
authors.initials: PKT
authors.lastname: Lin
authors.affiliation: Nestlé Research Centre, 1000 Lausanne, Switzerland.
authors.forename: Sean
authors.initials: S
authors.lastname: Austin
mesh_terms: D005526:Food, Formulated / Q000032:analysis*; D005630:Fructans / Q000032:analysis*; D006868:Hydrolysis; D041943:Infant Formula / Q000032:analysis*; D007753:Laboratories
chemical_list: D005630:Fructans