title: Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress.
abstract: Low temperature is an important environmental factor in aquaculture farming that affects the survival and growth of organisms. In the present study, we investigated the effects of low temperature on biochemical parameters, oxidative stress and apoptosis in pufferfish. In the stress group, water temperature decreased from 25 °C to 13 °C at a rate of 1 °C/1 h. Fish blood and liver were collected to assay biochemical parameters, oxidative stress and expression of genes at 25 °C, 21 °C, 17 °C, 13 °C and 13 °C for 24 h. The results showed that low temperature could decrease total blood cell count, inhibit cell viability, and subsequently lead to DNA damage. Biochemical parameters such as plasma protein and ALP significantly declined in fish under low temperature, while a significant increase in AST, ALT, LDH and glucose was observed. The gene expression of antioxidant enzymes (SOD and CAT), HSP90 and C3 were induced by low temperature stress. Furthermore, the gene expression of apoptotic related genes including P53, caspase-9 and caspase-3 were up-regulated, suggesting that caspase-dependent pathway could play important roles in low temperature-induced apoptosis in fish. This study may provide baseline information about how cold stress affects the physiological responses and apoptosis in fish.
authors.affiliation: Key Laboratory of Aquatic Product Processing, Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510300, PR China. Electronic address: chengchanghong09@163.com.
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Chao-Xia
authors.initials: CX
authors.lastname: Ye
authors.affiliation: Key Laboratory of Aquatic Product Processing, Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510300, PR China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center (SCS-REPIC), PR China. Electronic address: guozhixun1@163.com.
authors.forename: Zhi-Xun
authors.initials: ZX
authors.lastname: Guo
authors.affiliation: Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou 510631, PR China. Electronic address: wanganl@scnu.edu.cn.
authors.forename: An-Li
authors.initials: AL
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D003080:Cold Temperature; D004249:DNA Damage; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress; D011897:Random Allocation; D023741:Takifugu / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000502:physiology*
chemical_list: D000975:Antioxidants; D029941:Fish Proteins
keywords: Apoptosis; Cold stress; Oxidative stress; Takifugu obscurus