title: The effect of starvation and re-feeding on mitochondrial potential in the midgut of Neocaridina davidi (Crustacea, Malacostraca).
abstract: The midgut in the freshwater shrimp Neocaridina davidi (previously named N. heteropoda) (Crustacea, Malacostraca) is composed of a tube-shaped intestine and a large hepatopancreas that is formed by numerous blind-ended tubules. The precise structure and ultrastructure of these regions were presented in our previous papers, while here we focused on the ultrastructural changes that occurred in the midgut epithelial cells (D-cells in the intestine, B- and F- cells in the hepatopancreas) after long-term starvation and re-feeding. We used transmission electron microscopy, light and confocal microscopes and flow cytometry to describe all of the changes that occurred due to the stressor with special emphasis on mitochondrial alterations. A quantitative assessment of cells with depolarized mitochondria helped us to establish whether there is a relationship between starvation, re-feeding and the inactivation/activation of mitochondria. The results of our studies showed that in the freshwater shrimp N. davidi that were analyzed, long-term starvation activates the degeneration of epithelial cells at the ultrastructural level and causes an increase of cells with depolarized (non-active) mitochondria. The process of re-feeding leads to the gradual regeneration of the cytoplasm of the midgut epithelial cells; however, these changes were observed at the ultrastructural level. Additionally, re-feeding causes the regeneration of mitochondrial ultrastructure. Therefore, we can state that the increase in the number of cells with polarized mitochondria occurs slowly and does not depend on ultrastructural alterations.
authors.affiliation: University of Silesia, Department of Animal Histology and Embryology, Katowice, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Włodarczyk
authors.affiliation: University of Silesia, Department of Animal Histology and Embryology, Katowice, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Sonakowska
authors.affiliation: University of Silesia, Department of Animal Histology and Embryology, Katowice, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Kamińska
authors.affiliation: University of Silesia, Department of Animal Histology and Embryology, Katowice, Poland.
authors.forename: Angelika
authors.initials: A
authors.lastname: Marchewka
authors.affiliation: University of Silesia, Department of Animal Physiology and Ecotoxicology, Katowice, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Wilczek
authors.affiliation: Heart Prosthesis Institute, Bioengineering Laboratory, Zabrze, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Wilczek
authors.affiliation: Silesian University of Technology, Institute of Automatic Control, Faculty of Automatic Control, Electronics and Computer Science, Gliwice, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Student
authors.affiliation: University of Silesia, Department of Animal Histology and Embryology, Katowice, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rost-Roszkowska
mesh_terms: D000818:Animals; D003445:Crustacea / Q000378:metabolism*; D004435:Eating; D007413:Intestinal Mucosa / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism*; D013217:Starvation / Q000378:metabolism*