title: Diterpenoids with thioredoxin reductase inhibitory activities from Jatropha multifida.
abstract: Chemical investigation of the Jatropha multifida has led to the isolation of nine diterpenoids (1-9), including a new jatromulone A, four podocarpane diterpenoids (2-5), two lathyrane-type diterpenoids (6 and 7) and two dinorditerpenoids (8 and 9). Their structures were elucidated by spectroscopic analysis, and the absolute configurations of 1 were determined by CD analysis. All of the diterpenoids were screened for inhibitory activity against thioredoxin reductase (TrxR), which is a potential target for cancer chemotherapy with redox balance and antioxidant functions. Compounds 6 and 7 exhibited stronger activity (IC50: 23.4 and 10.6 μM, respectively) than the positive control, curcumin (IC50= 25.0 μM). Compounds 2-9 were isolated from J. multifida for the first time.
authors.affiliation: a Central Laboratory , Seventh People's Hospital of Shanghai University of TCM , Shanghai , P. R. China.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: a Central Laboratory , Seventh People's Hospital of Shanghai University of TCM , Shanghai , P. R. China.
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: b School of Pharmacy , Chengdu University of Traditional Chinese Medicine , Chengdu , P. R. China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Dai
authors.affiliation: c Faculty of Science, School of Biomolecular Sciences , Liverpool John Moores University , Liverpool , UK.
authors.forename: Khalid
authors.initials: K
authors.lastname: Rahman
authors.affiliation: a Central Laboratory , Seventh People's Hospital of Shanghai University of TCM , Shanghai , P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D031286:Jatropha / Q000737:chemistry*; D015394:Molecular Structure; D013880:Thioredoxin-Disulfide Reductase / Q000037:antagonists & inhibitors*
chemical_list: D004224:Diterpenes; D004791:Enzyme Inhibitors; C530347:lathyrane; D013880:Thioredoxin-Disulfide Reductase
keywords: Jatropha multifida; TrxR inhibitory activity; dinorditerpenoid; lathyrane; podocarpane