title: Roles of GATA6 during Gonadal Development in Japanese Flounder: Gonadogenesis, Regulation of Gender-Related Genes, Estrogen Formation and Gonadal Function Maintenance.
abstract: GATA-binding protein 6 (GATA6), a highly-conserved transcription factor of the GATA family plays an important role in gonadal cell proliferation, differentiation and endoderm development. In this study, the full-length cDNA of GATA6 ofParalichthys olivaceus(Japanese flounder) was obtained. Phylogenetic, gene structure and synteny analyses demonstrated that GATA6 ofP. olivaceusis homologous to that of teleosts and tetrapods. TheP. olivaceusGATA6 transcript showed higher expression in testis than in ovary, demonstrating a sexually dimorphic gene expression. During embryonic development, the expression ofP. olivaceusGATA6 increased at the blastula stage, demonstrating that GATA6 is involved in morphogenesis. Results of in situ hybridization showed that GATA6 signals were detected in Sertoli cells, oogonia and oocytes. Moreover, 17α methyl testosterone, a male hormone, could moderately upregulateP. olivaceusGATA6 and downregulateP. olivaceusaromatase CYP19A1 in testis cells. These results suggest that GATA6 may play an important role in gonadal development inP. olivaceus. This study provides valuable information on the function ofP. olivaceusGATA6, laying the foundation for further development of breeding techniques in this species.
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. lizanlxm@163.com.
authors.forename: Zan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. xiumei0210@163.com.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. sy18306421070@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. ljkmn911@outlook.com.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. yuezhong_liu@163.com.
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. wang_mengxun@163.com.
authors.forename: Mengxun
authors.initials: M
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. qzhang@ouc.edu.cn.
authors.forename: Quanqi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. wangxubo@ouc.edu.cn.
authors.forename: Xubo
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002478:Cells, Cultured; D002875:Chromosomes / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005432:Flounder / Q000196:embryology* / Q000235:genetics*; D050993:GATA6 Transcription Factor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D016678:Genome; D006066:Gonads / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology; D010802:Phylogeny; D000072417:Protein Domains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012727:Sex Characteristics; D040681:Structural Homology, Protein; D026801:Synteny; D013737:Testis / Q000166:cytology
chemical_list: D018076:DNA, Complementary; D004967:Estrogens; D050993:GATA6 Transcription Factor; D012333:RNA, Messenger; D001141:Aromatase; D008777:Methyltestosterone
keywords: GATA6; Paralichthys olivaceus; dimorphic expression; gonadal development; hormone stimulation