title: In VitroAcid-Mediated Initial Dental Enamel Loss Is Associated with Genetic Variants Previously Linked to Caries Experience.
abstract: We have previously shown thatAQP5andBTF3genetic variation and expression in whole saliva are associated with caries experience suggesting that these genes may have a functional role in protecting against caries. To further explore these results, we testedex vivoif variants in these genes are associated with subclinical dental enamel mineral loss. DNA and enamel samples were obtained from 53 individuals. Enamel samples were analyzed for Knoop hardness of sound enamel, integrated mineral loss after subclinical carious lesion creation, and change in integrated mineral loss after remineralization. DNA samples were genotyped for single nucleotide polymorphisms using TaqMan chemistry. Chi-square and Fisher's exact tests were used to compare individuals above and below the mean sound enamel microhardness of the cohort with alpha of 0.05. The A allele ofBTF3rs6862039 appears to be associated with harder enamel at baseline (p= 0.09), enamel more resistant to demineralization (p= 0.01), and enamel that more efficiently regain mineral and remineralize (p= 0.04). Similarly, the G allele ofAQP5marker rs3759129 and A allele ofAQP5marker rs296763 are associated with enamel more resistant to demineralization (p= 0.03 and 0.05, respectively).AQP5andBTF3genetic variations influence the initial subclinical stages of caries lesion formation in the subsurface of enamel.
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University of PittsburghPittsburgh, PA, USA; Department of Pediatric Dentistry, School of Dental Medicine, University of PittsburghPittsburgh, PA, USA.
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Vieira
authors.affiliation: Department of Pedodontics, School of Dentistry, Medipol Istanbul University Istanbul, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Bayram
authors.affiliation: Department of Pedodontics, School of Dentistry, Istanbul University Istanbul, Turkey.
authors.forename: Figen
authors.initials: F
authors.lastname: Seymen
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University of Pittsburgh Pittsburgh, PA, USA.
authors.forename: Regina C
authors.initials: RC
authors.lastname: Sencak
authors.affiliation: Department of Cariology, Operative Dentistry and Dental Public Health, School of Dentistry, Indiana University Indianapolis, IN, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Lippert
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University of PittsburghPittsburgh, PA, USA; Department of Pediatric Dentistry, School of Dental Medicine, University of PittsburghPittsburgh, PA, USA.
authors.forename: Adriana
authors.initials: A
authors.lastname: Modesto
keywords: aquaporins; dental caries; dental enamel; dental erosion; transcription factors