title: Accumulation of Flavonoid Glycosides and UFGT Gene Expression in Mulberry Leaves (Morus alba L.) before and after Frost.
abstract: In order to determine the molecular mechanism underlying the influence of frost on chemical changes in mulberry leaves, the UFGT activity, expression level, and accumulation of flavonoid glycosides in mulberry leaves (Morus alba L.) were studied. The expression of UFGT gene was investigated by quantitative real-time PCR (qRT-PCR) and the UFGT activity, accumulation of flavonoid glycosides was studied by high performance liquid chromatography. Then, the correlation between the expression level of UFGT, the UFGT activity, and the flavonoid glycosides accumulation with temperature was explored. The accumulation of isoquercitrin and astragalin is significantly positively correlated with UFGT gene expression and UFGT activity. On the contrary, the average temperature was significantly negatively correlated with the level of UFGT gene expression and UFGT activity. The results show that after frost, low temperature can induce the expression of UFGT gene in mulberry leaves, resulting in the accumulation of flavonoid glycosides.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China.|College of Pharmacy, Jiangsu University, Zhenjiang, 212013, P. R. China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Pharmacy, Jiangsu University, Zhenjiang, 212013, P. R. China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Pharmacy, Jiangsu University, Zhenjiang, 212013, P. R. China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Fan
authors.affiliation: College of Pharmacy, Jiangsu University, Zhenjiang, 212013, P. R. China.
authors.forename: Dujun
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Jiangsu University, Zhenjiang, 212013, P. R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Gong
authors.affiliation: College of Pharmacy, Jiangsu University, Zhenjiang, 212013, P. R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ouyang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D044949:Kaempferols / Q000378:metabolism; D031625:Morus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D005419:Flavonoids; D006027:Glycosides; D044949:Kaempferols; D010940:Plant Proteins; C016527:isoquercitrin; D012313:RNA; D011794:Quercetin; C001579:astragalin
keywords: 
  UFGT
  ; Flavonoid glycoside; Frost; Mulberry leaf; qRT-PCR