title: Screening of Peruvian Medicinal Plants for Tyrosinase Inhibitory Properties: Identification of Tyrosinase Inhibitors in Hypericum laricifolium Juss.
abstract: Tyrosinase inhibitors are of far-ranging importance in cosmetics, medicinal products, and food industries. Peru is a diverse country with a wide variety of plants that may contain excellent anti-tyrosinase inhibitors. In the present study, the tyrosinase inhibitory properties of 50 medicinal plant extracts from Peru were investigated using tyrosinase assay. Among plant extracts, those that showed an inhibition rate >50% wereHypericum laricifoliumJuss., Taraxacum officinaleF.H.Wigg.,andMuehlenbeckia vulcanicaMeisn.,withH. laricifoliumJuss. showing the greatest anti-tyrosinase activity. AlthoughH. laricifoliumJuss. has been widely used as a medicinal plant by Peruvians, little is known regarding its bioactive components and effects on tyrosinase activity. For this reason, we attempted to discover tyrosinase inhibitors inH. laricifoliumJuss. for the first time. The bioactive components were separated by Sephadex LH-20 chromatography and eluted with 100% methanol. Eight compounds were discovered and characterized by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD): protocatechuic acid,p-hydroxybenzoic acid, chlorogenic acid, vanilic acid, caffeic acid, kaempferol 3-O-glucuronide, quercetin, and kaempferol. In addition, the concentration of these compounds required for 50% inhibition (IC50) of tyrosinase activity were evaluated. Quercetin exhibited the strongest tyrosinase inhibition (IC5014.29 ± 0.3 μM). Therefore, the Peruvian plantH. laricifoliumJuss. could be a novel source for anti-tyrosinase activity.
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. estreyany@gmail.com.
authors.forename: Yanymee Nimesia Guillen
authors.initials: YN
authors.lastname: Quispe
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. isohsh@gmail.com.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. wangzq01234@gmail.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. limss@hallym.ac.kr.|Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. limss@hallym.ac.kr.|Institute of Korean Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea. limss@hallym.ac.kr.
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
mesh_terms: D004791:Enzyme Inhibitors / Q000032:analysis / Q000494:pharmacology; D020902:Hypericum / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010568:Peru; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000302:isolation & purification
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011794:Quercetin; D014442:Monophenol Monooxygenase
keywords: Hypericum laricifolium Juss.; Peruvian plants; screening; tyrosinase