title: In vitro assessment of the indirect antioxidant activity of Sulforaphane in redox imbalance vanadium-induced.
abstract: Owing to sulforaphane presence, a dietary consumption of Brassicaceae prevents chronic diseases. This hormetic compound induces adaptive stress response at subtoxic doses, while doses that exceed the cellular defence are toxic. In HepG2, Caco-2 and Vero cells, we investigated the sulforaphane (SFN) (5 μM) role in counteracting redox imbalance induced by VOSO4[V(IV)]. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test showed a dose-dependent viability reduction (r < -0.95; p < 0.01) (range 5-80 μM). At 5 μM, SFN enhancement of mitochondrial activity was confirmed by Δψm (p < 0.05) both in basal condition and in redox-stressed cells. Intracellular ROS, DNA and lysosomal oxidative damages underlined the indirect antioxidant SFN activity, confirmed by the increase of GSH. The SFN empowering effects on mitochondrial function were imputable to the presence of mitochondrial proteins among the Nrf2-responsive phase II proteins. Considering the link between oxidative stress and chronic diseases, a long-term dietary intake of Brassicaceae could be strongly advisable.
authors.affiliation: a Department of Biomedical and Dental Sciences and Morphofunctional Imaging , University of Messina , Messina , Italy.
authors.forename: Giuseppa
authors.identifier: http://orcid.org/0000-0002-9072-1148
authors.initials: G
authors.lastname: Visalli
authors.affiliation: a Department of Biomedical and Dental Sciences and Morphofunctional Imaging , University of Messina , Messina , Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Facciolà
authors.affiliation: a Department of Biomedical and Dental Sciences and Morphofunctional Imaging , University of Messina , Messina , Italy.
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Bertuccio
authors.affiliation: a Department of Biomedical and Dental Sciences and Morphofunctional Imaging , University of Messina , Messina , Italy.
authors.forename: Isa
authors.initials: I
authors.lastname: Picerno
authors.affiliation: a Department of Biomedical and Dental Sciences and Morphofunctional Imaging , University of Messina , Messina , Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Di Pietro
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D017879:Isothiocyanates / Q000008:administration & dosage / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013454:Sulfoxides; D017968:Vanadium Compounds / Q000633:toxicity; D014709:Vero Cells
chemical_list: D000975:Antioxidants; D017879:Isothiocyanates; D017382:Reactive Oxygen Species; D013454:Sulfoxides; D017968:Vanadium Compounds; C034028:vanadyl sulfate; C016766:sulforaphane
keywords: Caco-2; HepG2; Sulforaphane; Vero cells; mitochondrial function; oxidative stress; vanadium V(IV)