title: Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration.
abstract: Oxygen tension is an important micro-environmental factor that affects epidermal development and function. After injury, high oxygen consumption and vascular injury result in partial hypoxia. However, whether hypoxia benefits or hurts wound healing remains controversial. In this study, a tissue oxygen tension monitor was used to detect the spatial and temporal distribution of oxygen in burn wounds. In vitro, we demonstrate that hypoxia promoted the expression of integrin β1 and the migration of keratinocytes. Furthermore, hypoxia-induced migration was slowed by Notch1 ligands and a siRNA against ITGB1 (integrin β1). Our findings suggest that integrin β1 may be an oxygen-sensitive molecule that promotes keratinocyte migration during wound healing and that Notch1 signaling is involved in this process.
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Di
authors.initials: D
authors.lastname: Tang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Yan
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Xupin
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology*; D002465:Cell Movement; D000860:Hypoxia; D019012:Integrin beta1 / Q000378:metabolism*; D015603:Keratinocytes / Q000502:physiology*; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000032:analysis; D051881:Receptor, Notch1 / Q000378:metabolism*; D015398:Signal Transduction; D062211:Spatio-Temporal Analysis; D014945:Wound Healing
chemical_list: D019012:Integrin beta1; D051881:Receptor, Notch1; D010100:Oxygen