title: Production of Curcuminoids inEngineered Escherichia coli.
abstract: Curcumin, a hydrophobic polyphenol derived from the rhizome of the herbCurcuma longa, possesses diverse pharmacological properties, including anti-inflammatory, antioxidant, antiproliferative, and antiangiogenic activities. Two curcuminoids (dicinnamoylmethane and bisdemethoxycurcumin) were synthesized from glucose inEscherichia coli.PAL(phenylalanine ammonia lyase) orTAL(tyrosine ammonia lyase), along withOs4CL(p-coumaroyl-CoA ligase) andCUS(curcumin synthase) genes, were introduced intoE. coli, and each strain produced dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids inE. coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, was engineered. Using the engineered strains, the production of bisdemethoxycurcumin increased from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l.
authors.affiliation: Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Cha
authors.affiliation: Department of Forest Resources, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea.
authors.forename: Bog-Gyu
authors.initials: BG
authors.lastname: Kim
authors.affiliation: Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Joong-Hoon
authors.initials: JH
authors.lastname: Ahn
mesh_terms: D000642:Ammonia-Lyases / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics; D053898:Biosynthetic Pathways / Q000235:genetics; D003470:Culture Media; D003474:Curcumin / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D036381:Diarylheptanoids; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D008025:Ligases / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D012765:Shikimic Acid / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
chemical_list: D003470:Culture Media; D036381:Diarylheptanoids; C547081:dicinnamoylmethane; D012765:Shikimic Acid; C034786:bisdemethoxycurcumin; D014443:Tyrosine; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; D010650:Phenylalanine Ammonia-Lyase; D008025:Ligases; C516709:curcuminoid synthase, Curcuma longa; D003474:Curcumin; D005947:Glucose
keywords: Bisdemethoxycurcumin; dicinnamoylmethane; metabolic engineering