title: Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon.
abstract: This study investigates the behaviour of Saccharomyces cerevisiae strains, in order to obtain insight into the intraspecies competition taking place in mixed populations of this species. Two strains of S. cerevisiae, one dominant and one non-dominant, were labelled and mixed, and individual fermentations were set up to study the transcriptomes of the strains by means of RNA-seq. The results obtained suggest that cell-to-cell contact and aggregation, which are driven by the expression of genes that are associated with the cell surface, are indispensable conditions for the achievement of dominance. Observations on mixed aggregates, made up of cells of both strains, which were detected by means of flow cytometry, have confirmed the transcriptomic data. Furthermore, overexpression of the SSU1 gene, which encodes for a transporter that confers resistance to sulphites, provides an ecological advantage to the dominant strain. A mechanistic model is proposed that sheds light on the dominance phenomenon between different strains of the S. cerevisiae species. The collected data suggest that cell-to-cell contact, together with differential sulphite production and resistance is important in determining the dominance of one strain over another.
authors.affiliation: Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC. Food Biotechnology Department. Institute of Agrochemistry and Food Technology (IATA-CSIC) Avda. Agustín Escardino, 7. E-46980 Paterna (Valencia), Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pérez-Torrado
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Rantsiou
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.
authors.forename: Benedeta
authors.initials: B
authors.lastname: Perrone
authors.affiliation: Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC. Food Biotechnology Department. Institute of Agrochemistry and Food Technology (IATA-CSIC) Avda. Agustín Escardino, 7. E-46980 Paterna (Valencia), Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Navarro-Tapia
authors.affiliation: Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC. Food Biotechnology Department. Institute of Agrochemistry and Food Technology (IATA-CSIC) Avda. Agustín Escardino, 7. E-46980 Paterna (Valencia), Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Querol
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Cocolin
mesh_terms: D005285:Fermentation; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D010087:Oxides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013457:Sulfur Compounds / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010087:Oxides; D013457:Sulfur Compounds; C405951:sulfur monoxide