title: Identification of candidate diagnostic serum biomarkers for Kawasaki disease using proteomic analysis.
abstract: Kawasaki disease (KD) is a systemic vasculitis and childhood febrile disease that can lead to cardiovascular complications. The diagnosis of KD depends on its clinical features, and thus it is sometimes difficult to make a definitive diagnosis. In order to identify diagnostic serum biomarkers for KD, we explored serum KD-related proteins, which differentially expressed during the acute and recovery phases of two patients by mass spectrometry (MS). We identified a total of 1,879 proteins by MS-based proteomic analysis. The levels of three of these proteins, namely lipopolysaccharide-binding protein (LBP), leucine-rich alpha-2-glycoprotein (LRG1), and angiotensinogen (AGT), were higher in acute phase patients. In contrast, the level of retinol-binding protein 4 (RBP4) was decreased. To confirm the usefulness of these proteins as biomarkers, we analyzed a total of 270 samples, including those collected from 55 patients with acute phase KD, by using western blot analysis and microarray enzyme-linked immunosorbent assays (ELISAs). Over the course of this experiment, we determined that the expression level of these proteins changes specifically in the acute phase of KD, rather than the recovery phase of KD or other febrile illness. Thus, LRG1 could be used as biomarkers to facilitate KD diagnosis based on clinical features.
authors.affiliation: Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Department of Pediatrics, Yokohama City University School of Medicine, Yokohama, Japan.|Department of Pediatrics, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Yanagimachi
authors.affiliation: Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ino
authors.affiliation: Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
authors.forename: Mao
authors.initials: M
authors.lastname: Aketagawa
authors.affiliation: Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
authors.forename: Michie
authors.initials: M
authors.lastname: Matsuo
authors.affiliation: Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Okayama
authors.affiliation: Department of Pediatrics, Yokohama City University Medical Center, Yokohama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: Department of Pediatrics, Showa General Hospital, Tokyo, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Oba
authors.affiliation: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Morioka
authors.affiliation: Department of Infectious Disease &Immunology, Kanagawa Children's Medical Center, Yokohama, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: Teikyo Academic Research Center, Teikyo University, Tokyo, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Department of Pediatrics, Yokohama City University School of Medicine, Yokohama, Japan.
authors.forename: Shumpei
authors.initials: S
authors.lastname: Yokota
authors.affiliation: Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hirano
authors.affiliation: Department of Pediatrics, Yokohama City University Medical Center, Yokohama, Japan.|Department of Lifetime Clinical Immunology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Mori
mesh_terms: D000293:Adolescent; D015415:Biomarkers; D001798:Blood Proteins; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009080:Mucocutaneous Lymph Node Syndrome / Q000097:blood* / Q000175:diagnosis*; D011379:Prognosis; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome