title: Use of a cocktail of spin traps for fingerprinting large range of free radicals in biological systems.
abstract: It is well established that the formation of radical species centered on various atoms is involved in the mechanism leading to the development of several diseases or to the appearance of deleterious effects of toxic molecules. The detection of free radical is possible using Electron Paramagnetic Resonance (EPR) spectroscopy and the spin trapping technique. The classical EPR spin-trapping technique can be considered as a "hypothesis-driven" approach because it requires an a priori assumption regarding the nature of the free radical in order to select the most appropriate spin-trap. We here describe a "data-driven" approach using EPR and a cocktail of spin-traps. The rationale for using this cocktail was that it would cover a wide range of biologically relevant free radicals and have a large range of hydrophilicity and lipophilicity in order to trap free radicals produced in different cellular compartments. As a proof-of-concept, we validated the ability of the system to measure a large variety of free radicals (O-, N-, C-, or S- centered) in well characterized conditions, and we illustrated the ability of the technique to unambiguously detect free radical production in cells exposed to chemicals known to be radical-mediated toxic agents.
authors.affiliation: Louvain Drug Research Institute, Biomedical Magnetic Resonance Research Group, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Valérie
authors.initials: V
authors.lastname: Marchand
authors.affiliation: Louvain Drug Research Institute, Biomedical Magnetic Resonance Research Group, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Charlier
authors.affiliation: Louvain Drug Research Institute, Toxicology and Cancer Biology Research Group, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Julien
authors.initials: J
authors.lastname: Verrax
authors.affiliation: Louvain Drug Research Institute, Toxicology and Cancer Biology Research Group, Université catholique de Louvain, Brussels, Belgium.|Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique, Chile.
authors.forename: Pedro
authors.initials: P
authors.lastname: Buc-Calderon
authors.affiliation: Louvain Drug Research Institute, Biomedical Magnetic Resonance Research Group, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Levêque
authors.affiliation: Louvain Drug Research Institute, Biomedical Magnetic Resonance Research Group, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Bernard
authors.initials: B
authors.lastname: Gallez
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D013113:Spin Labels
chemical_list: D005609:Free Radicals; D013113:Spin Labels