title: Bone marrow-derived mesenchymal stem cells mitigate caspase-3 and 8-hydroxy proline induced via β-adrenergic agonist in pulmonary injured rats.
abstract: Estimating the ability of bone marrow-derived mesenchymal stem cells (BM-MSCs) to alleviate pulmonary injury induced via isoproterenol (ISP). ISP was injected in a dose of (100 mg/kg, subcutaneously twice at an interval of 24 h). One month post BM-MSCs transplantation by intravenous injection, pulmonary oxidative stress was assessed, and Western blot analyses and histopathological investigations were conducted. Compared with the normal control group, BM-MSCs transplantation significantly decreased the expression of pulmonary anti-oxidative stress marker. Western blot analysis revealed that ISP significantly reduced the protein expression of the anti-oxidative stress marker nuclear related factor-2 (Nrf2). However, the apoptotic marker (caspase-3) and collagen content marker (8-hydroxyproline) were markedly elevated. These biochemical markers were confirmed by histopathological investigations. Finally, it was demonstrated that BM-MSCs transplantation showed a superior effect in improving pulmonary function through alleviating oxidative stress, apoptosis, and collagen content.
authors.affiliation: Biochemistry, Department of Therapeutic Chemistry, National Research Center, Dokki, Giza, Egypt.
authors.forename: Mai O
authors.initials: MO
authors.lastname: Kadry
authors.affiliation: Molecular Biology, Department of Therapeutic Chemistry, Dokki, Giza, Egypt.
authors.forename: Rehab M
authors.initials: RM
authors.lastname: Abdel-Megeed
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000009:adverse effects* / Q000494:pharmacology; D064591:Allografts; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D006909:Hydroxyproline / Q000378:metabolism*; D007545:Isoproterenol / Q000009:adverse effects* / Q000494:pharmacology; D055370:Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D051381:Rats
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D015415:Biomarkers; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; C505410:Casp3 protein, rat; D053148:Caspase 3; D007545:Isoproterenol; D006909:Hydroxyproline
keywords: 8-hydroxyl proline; BM-MSCs; Western blot; caspase-3