title: The role of nesfatin-1 expression in letrozole-induced polycystic ovaries in the rat.
abstract: Polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disorder, generally exhibiting the characteristic features of hyperandrogenemia, insulin resistance (IR) and obesity. Nesfatin-1 is derived from the precursor nucleobindin2 (NUCB2), and plays an active role in energy balance, glucose metabolism and most likely gonadal function. In order to explore the role of nesfatin-1, we employed a rat model that uses letrozole to induce PCOS. The PCOS rats exhibited increased body weight, irregular cycles, polycystic ovaries characterized by cysts formed from atretic follicles, and a diminished granulosa layer. The expression of both nesfatin-1 mRNA and protein in the ovarian tissues of PCOS group decreased significantly compared to the control group (p < 0.05). Nesfatin-1 expression in peripheral blood also decreased in the PCOS group, in contrast with the control group. Furthermore, we found that nesfatin-1 had a positive correlation with FSH, E2and P, whereas it had a negative correlation with LH, and total T (p < 0.05). When taken together, these data indicated that the decrease in nesfatin-1 may contribute to the mechanism governing PCOS, and might provide a new potential target for therapies aimed at treating PCOS.
authors.affiliation: a Binzhou Medical University , Yantai , China and.
authors.forename: Yingqiao
authors.initials: Y
authors.lastname: Xu
authors.affiliation: a Binzhou Medical University , Yantai , China and.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: b Department of Reproductive Medicine , Binzhou Medical University Hospital , Binzhou , China.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Li
authors.affiliation: b Department of Reproductive Medicine , Binzhou Medical University Hospital , Binzhou , China.
authors.forename: Kaixue
authors.initials: K
authors.lastname: Lao
authors.affiliation: b Department of Reproductive Medicine , Binzhou Medical University Hospital , Binzhou , China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D000077289:Letrozole; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009570:Nitriles; D000080866:Nucleobindins; D010053:Ovary / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced / Q000378:metabolism*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D014230:Triazoles
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D009570:Nitriles; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D014230:Triazoles; D000077289:Letrozole
keywords: Hormonal changes; nesfatin-1; nucleobindin2; polycystic ovary syndrome; rat model