title: Propionibacterium acnesas a possible pathogen of granuloma in patients with ocular sarcoidosis.
abstract: To detectPropionibacterium acnesfrom intraocular granuloma in patients with ocular sarcoidosis.
  
  
  
  Ten patients (10 eyes) with uveitis associated with sarcoidosis, who underwent vitrectomy to remove the epiretinal membrane were analysed. The patients were 70.4±7.6 (mean±SD) years of age, and the observation period from diagnosis of sarcoidosis until vitrectomy was 27.6±15.1 months. Histopathological examination of paraffin-embedded sections of the excised epiretinal membranes was conducted by H&E staining and immunohistochemical staining using PAB antibody, which is a monoclonal antibody againstP. acnes. Four patients with idiopathic epiretinal membrane and four patients with epiretinal membrane caused by chronic uveitis other than sarcoidosis were included as control.
  
  
  
  Granuloma in the epiretinal membrane was observed in 4 of 10 patients with sarcoidosis, and all the granulomas were positive for PAB. In one patient without granuloma in the epiretinal membrane, PAB immunoreactivity was found in inflammatory cells, presumably macrophages. No granuloma and no PAB immunoreactivity were detected in all control patients, except one with chronic uveitis in whom faint PAB immunoreactivity was detected in inflammatory cells.
  
  
  
  P. acneswas detected from granulomas formed in the epiretinal membrane of patients with sarcoidosis. The significance ofP. acnesin the pathogenesis of uveitis associated with sarcoidosis remains to be determined.
authors.affiliation: Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Goto
authors.affiliation: Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Usui
authors.affiliation: Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Umazume
authors.affiliation: Department of Human Pathology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Uchida
authors.affiliation: Department of Human Pathology, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Eishi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019773:Epiretinal Membrane / Q000150:complications / Q000175:diagnosis / Q000601:surgery; D015818:Eye Infections, Bacterial / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D005260:Female; D005500:Follow-Up Studies; D016908:Gram-Positive Bacterial Infections / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D006099:Granuloma / Q000175:diagnosis / Q000209:etiology* / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011425:Propionibacterium acnes / Q000302:isolation & purification*; D012507:Sarcoidosis / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D014605:Uveitis / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D014821:Vitrectomy
keywords: Inflammation; Pathology