title: High production of xanthan gum by a glycerol-tolerant strain Xanthomonas campestris WXLB-006.
abstract: The superior properties of xanthan gum make it an industrial aginomoto used in many industries, especially in oil recovery. In the present work, xanthan production from glycerol by a mutant strain Xanthomonas campestris WXLB-006 reached as high as 17.8 g/L in flask culture. With the adoption of pH control, varied aeration and agitation, and varied glycerol feeding strategy, xanthan production reached 33.9 g/L in a 7-L fermenter and fermentation time decreased to 60 hr. Instead of difficultly and costly purifying glycerol, this research provides a very good case for glycerol utilization. At the same time, this is the first report on a high glycerol-tolerant strain for microbial polysaccharide production and 33.9 g/L is the highest production of xanthan gum produced from glycerol so far.
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Zichao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Wu
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Gao
authors.affiliation: b Wuxi Galaxy Biotech Co. Ltd. , Wuxi , Jiangsu , China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: a The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , Jiangsu , China.
authors.forename: Xiao-Bei
authors.initials: XB
authors.lastname: Zhan
mesh_terms: D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D016959:Xanthomonas campestris / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; D005990:Glycerol; C002563:xanthan gum
keywords: Adaptive evolution; fermentation strategy; glycerol; glycerol-tolerant strain; high production; xanthan gum