title: Isolation, culture and biological characteristics of multipotent porcine skeletal muscle satellite cells.
abstract: Skeletal muscle has a huge regenerative potential for postnatal muscle growth and repair, which mainly depends on a kind of muscle progenitor cell population, called satellite cell. Nowadays, the majority of satellite cells were obtained from human, mouse, rat and other animals but rarely from pig. In this article, the porcine skeletal muscle satellite cells were isolated and cultured in vitro. The expression of surface markers of satellite cells was detected by immunofluorescence and RT-PCR assays. The differentiation capacity was assessed by inducing satellite cells into adipocytes, myoblasts and osteoblasts. The results showed that satellite cells isolated from porcine tibialis anterior were subcultured up to 12 passages and were positive for Pax7, Myod, c-Met, desmin, PCNA and NANOG but were negative for Myogenin. Satellite cells were also induced to differentiate into adipocytes, osteoblasts and myoblasts, respectively. These findings indicated that porcine satellite cells possess similar biological characteristics of stem cells, which may provide theoretical basis and experimental evidence for potential therapeutic application in the treatment of dystrophic muscle and other muscle injuries.
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.|Sports Science College, Beijing Sports University, Beijing, 100084, People's Republic of China.
authors.forename: Jinjuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Kunfu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Xueting
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Physical Education, University of JiMei, Yingjiang Road, Jimei District, Xiamen, 361021, Fujian, People's Republic of China. yangxiaonuodexiao@163.com.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China. weijunguan301@gmail.com.
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D018482:Muscle, Skeletal / Q000166:cytology*; D012038:Regeneration / Q000502:physiology; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology; D013552:Swine
keywords: Biological characteristics; Multi-differentiation potential; Porcine; Satellite cells; Self-renewal