title: Preparation of astaxanthin-loaded DNA/chitosan nanoparticles for improved cellular uptake and antioxidation capability.
abstract: DNA/chitosan co-assemblies were initially used as nanocarriers for efficient astaxanthin encapsulation and delivery. The obtained astaxanthin-loaded DNA/chitosan (ADC) colloidal system was transparent and homogenous, with astaxanthin content up to 65μg/ml. Compared to free astaxanthin, ADC nanoparticles with an astaxanthin concentration as low as 3.35nM still showed a more powerful cytoprotective effect on H2O2-induced oxidative cell damage, and improved cell viability from 49.9% to 61.9%. The ROS scavenging efficiency of ADC nanoparticles was as high as 54.3%, which was 2-fold higher than that of free astaxanthin. Besides this, ADC nanoparticles were easily engulfed by Caco-2 cells in a short time, indicating that the encapsulated astaxanthin could be absorbed through endocytosis by intestinal epithelial cells. The improved antioxidation capability and facilitated cellular uptake enabled the ADC nanoparticles to be good candidates for efficient delivery and absorption of astaxanthin.
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China. Electronic address: wangqian366616552@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China. Electronic address: anxiaoying2010@163.com.
authors.forename: Yingyuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China. Electronic address: 2547141392@qq.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Guan
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China. Electronic address: 626517093@qq.com.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China. Electronic address: qfdang@ouc.edu.cn.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Dang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China. Electronic address: dongping@ouc.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China. Electronic address: lijouc@ouc.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China. Electronic address: liangxg@ouc.edu.cn.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Liang
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D001692:Biological Transport; D018938:Caco-2 Cells; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004705:Endocytosis; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D024341:Xanthophylls / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D024341:Xanthophylls; C005948:astaxanthine; D004247:DNA; D048271:Chitosan
keywords: Antioxidant; Astaxanthin; Cellular uptake; Chitosan; DNA; Nanodispersion; Nanoparticle