title: Lower concentrations of receptor for advanced glycation end products and epiregulin in amniotic fluid correlate to chemically induced cleft palate in mice.
abstract: This study investigated the correlation between differentially expressed proteins in amniotic fluid (AF) and cleft palate induced by all-trans retinoic acid (atRA), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice. Seven proteins were differentially expressed at embryonic day (E) 16.5 in atRA and control groups as revealed by label-based mouse antibody array. Enzyme-linked immunosorbent assay was further used to detect the expression levels of these proteins in AF from E13.5 to E16.5 in atRA, TCDD, and control groups. The cleft palate groups showed lower concentrations of receptor for advanced glycation end products (RAGE) and epiregulin at E16.5. RAGE immunostaining obviously decreased in palatal tissue sections obtained from E14.5 to E16.5 in the cleft palate groups as revealed by immunohistochemistry. These findings indicate that reduced levels of RAGE and epiregulin in AF are correlated to chemically induced cleft palate in mice.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China.
authors.forename: Xinhuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Fang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China. Electronic address: bianzhuan@whu.edu.cn.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Bian
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China. Electronic address: mengliuyan@whu.edu.cn.
authors.forename: Liuyan
authors.initials: L
authors.lastname: Meng
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002972:Cleft Palate / Q000139:chemically induced / Q000378:metabolism*; D004195:Disease Models, Animal; D066260:Epiregulin / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D008810:Mice, Inbred C57BL; D010159:Palate / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D011247:Pregnancy; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics / Q000378:metabolism*; D014212:Tretinoin / Q000633:toxicity
chemical_list: D015415:Biomarkers; D066260:Epiregulin; C587881:Ereg protein, mouse; D000072317:Polychlorinated Dibenzodioxins; D000067759:Receptor for Advanced Glycation End Products; D014212:Tretinoin
keywords: Amniotic fluid; Cleft palate; Epiregulin; Receptor for advanced glycation end products