title: Relation of dietary inorganic arsenic exposure and urinary inorganic arsenic metabolites excretion in Japanese subjects.
abstract: Inorganic arsenic (InAs) is a ubiquitous metalloid that has been shown to exert multiple adverse health outcomes. Urinary InAs and its metabolite concentration has been used as a biomarker of arsenic (As) exposure in some epidemiological studies, however, quantitative relationship between daily InAs exposure and urinary InAs metabolites concentration has not been well characterized. We collected a set of 24-h duplicated diet and spot urine sample of the next morning of diet sampling from 20 male and 19 female subjects in Japan from August 2011 to October 2012. Concentrations of As species in duplicated diet and urine samples were determined by using liquid chromatography-ICP mass spectrometry with a hydride generation system. Sum of the concentrations of urinary InAs and methylarsonic acid (MMA) was used as a measure of InAs exposure. Daily dietary InAs exposure was estimated to be 0.087 µg kg-1day-1(Geometric mean, GM), and GM of urinary InAs+MMA concentrations was 3.5 ng mL-1. Analysis of covariance did not find gender-difference in regression coefficients as significant (P > 0.05). Regression equation Log10[urinary InAs+MMA concentration] = 0.570× Log10[dietary InAs exposure level per body weight] + 1.15 was obtained for whole data set. This equation would be valuable in converting urinary InAs concentration to daily InAs exposure, which will be important information in risk assessment.
authors.affiliation: a Department of Environmental Studies , The University of Tokyo , Kashiwa , Chiba , Japan.|b National Institute for Environmental Studies , Tsukuba , Ibaraki , Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Oguri
authors.affiliation: a Department of Environmental Studies , The University of Tokyo , Kashiwa , Chiba , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshinaga
authors.affiliation: a Department of Environmental Studies , The University of Tokyo , Kashiwa , Chiba , Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: c National Institute of Advanced Industrial Science and Technology , Tsukuba , Ibaraki , Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tao
authors.affiliation: c National Institute of Advanced Industrial Science and Technology , Tsukuba , Ibaraki , Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakazato
mesh_terms: D000328:Adult; D001151:Arsenic / Q000032:analysis* / Q000633:toxicity* / Q000652:urine; D001152:Arsenicals / Q000652:urine; D015415:Biomarkers / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D004032:Diet; D004781:Environmental Exposure / Q000032:analysis*; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D018570:Risk Assessment
chemical_list: D001152:Arsenicals; D015415:Biomarkers; C020300:monomethylarsonic acid; D001151:Arsenic
keywords: Inorganic arsenic exposure; Japanese; biomarker; duplicate diet; spot urine