title: Ultra high performance liquid chromatography with tandem mass spectrometry for rapid separation and identification of main constituents in Chinese herbal formula Xuan Hu Suo San.
abstract: Xuan Hu Suo San (XHSS) is a traditional Chinese medicine that has been extensively applied in the treatment of osteoarthritis for many years, however, its chemical composition has not yet been elucidated. Thus, a rapid, efficient, and precise method based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was applied in both positive- and negative-ion modes to rapidly separate and identify the main chemical ingredients in XHSS for the first time. Finally, with the optimised separation and detection method, a total of 57 compounds were simultaneously separated within 13 min, among which 14 compounds were confirmed by comparing retention time and MS data with reference standards and others were tentatively identified by comparing with reference literatures. This rapid and sensitive approach is highly useful for the identification and characterisation of chemical constituents, and provides fundamental and extensive information supporting further metabolic and pharmacokinetic studies of XHSS.
authors.affiliation: a School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Huaqi
authors.initials: H
authors.lastname: Tang
authors.affiliation: a School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: a School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Shilin
authors.initials: S
authors.lastname: Luo
authors.affiliation: b Analytical and Testing Center , Beijing University of Chemical Technology , Beijing , China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shang
authors.affiliation: c College of Pharmacy , Inner Mongolia Medical University , Hohhot , China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.affiliation: d Pharmacy Department , Wangjing Hospital of China Academy of Chinese Medical Sciences , Beijing , China.
authors.forename: Jinghong
authors.initials: J
authors.lastname: Wang
authors.affiliation: a School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Duan
authors.affiliation: a School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gao
authors.affiliation: a School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
authors.forename: Yikun
authors.initials: Y
authors.lastname: Sun
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008516:Medicine, Chinese Traditional / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Constituents identification; Xuan Hu Suo San; rapid separation; traditional Chinese medicine; ultra high performance liquid chromatography