title: Analyzing Cytokinin Responses During Plant-Nematode Interactions.
abstract: Cyst nematodes are obligate biotrophs that induce the formation of a hypertrophied and hypermetabolic syncytial-feeding site in roots of the host plants. Cytokinin signaling is activated at the site of infection and contributes significantly to the formation of syncytium. Here, we describe a protocol for visualizing cytokinin signaling activation in Arabidopsis upon infection with cyst nematode Heterodera schachtii using promoter reporter lines, TCSn:GFP and ARR5:GUS.
authors.affiliation: Department of Molecular Phytomedicine, Rheinische Friedrich-Wilhelms-University of Bonn, Karlrobert-Kreiten-Strasse 13, 53115, Bonn, Germany.
authors.forename: Florian M W
authors.initials: FM
authors.lastname: Grundler
authors.affiliation: Department of Molecular Phytomedicine, Rheinische Friedrich-Wilhelms-University of Bonn, Karlrobert-Kreiten-Strasse 13, 53115, Bonn, Germany. siddique@uni-bonn.de.
authors.forename: Shahid
authors.initials: S
authors.lastname: Siddique
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000378:metabolism / Q000469:parasitology; D003583:Cytokinins / Q000378:metabolism*; D006790:Host-Parasite Interactions; D010935:Plant Diseases / Q000276:immunology / Q000469:parasitology; D010937:Plant Growth Regulators / Q000378:metabolism*; D010944:Plants / Q000378:metabolism* / Q000382:microbiology*; D030821:Plants, Genetically Modified; D014431:Tylenchoidea
chemical_list: D003583:Cytokinins; D010937:Plant Growth Regulators
keywords: ARR5:GUS; Cell cycle; Cytokinins; Giant cells; Nematode; Syncytium; TCSn:GFP