title: Gene expression of miRNA-138 and cyclin D1 in oral lichen planus.
abstract: OBJECTIVE
  This study aimed to evaluate microRNA-138 (miR-138) gene expression and its target cyclin D1 (CCND1) gene and protein expression in oral lichen planus (OLP) mucosa in an attempt to investigate their possible roles in OLP immunopathogenesis.
  
  
  METHODS
  Sixty oral biopsy specimens were harvested from 30 healthy subjects and 30 OLP patients, subdivided into reticular, atrophic, and erosive groups (n = 10 each). Samples were subjected to quantitative real-time polymerase chain reaction analysis for quantification of miR-138 and CCND1 relative gene expression and immunohistochemical analysis to determine CCND1 protein expression.
  
  
  RESULTS
  Samples from OLP patients had a significant underexpression of miR-138 gene and overexpression of CCND1 at both gene and protein levels compared to normal mucosa samples. The lowest levels of miR-138 expression were observed in atrophic and erosive OLP compared to reticular OLP, and the highest levels of CCND1 gene and protein expression were in atrophic OLP. An inverse correlation was demonstrated between the miR-138 expression and both CCND1 gene and protein expression in OLP patients. A significant positive correlation between CCND1 gene and protein expression was also observed.
  
  
  CONCLUSIONS
  Downregulation of miR-138 increases the gene and protein expression of its potential target CCND1 in OLP mucosa which might have a pivotal role in the disease pathogenesis.
  
  
  CONCLUSIONS
  This research implied that miR-138 may have a role in identification of symptomatic OLP lesions. MiR-138 might be considered as a potential tool in future OLP molecular therapy.
authors.affiliation: Department of Oral Medicine, Periodontology and Diagnosis, Faculty of Oral and Dental Medicine, Cairo University, 43 Zahraa Street, Dokki, Giza, Egypt. noha26@yahoo.com.
authors.forename: Noha A
authors.initials: NA
authors.lastname: Ghallab
authors.affiliation: Department of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt.
authors.forename: Rehab Fawzy
authors.initials: RF
authors.lastname: Kasem
authors.affiliation: Department of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt.
authors.forename: Safa Fathy Abd
authors.initials: SFA
authors.lastname: El-Ghani
authors.affiliation: Department of Medical Biochemistry& Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt.
authors.forename: Olfat G
authors.initials: OG
authors.lastname: Shaker
mesh_terms: D000368:Aged; D001706:Biopsy; D019938:Cyclin D1 / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D017676:Lichen Planus, Oral / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D019938:Cyclin D1
keywords: Cyclin D1; Oral lichen planus; Premalignant lesion; Tumor suppressor; miRNA-138