title: Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits.
abstract: Using single-molecule approaches, we directly observed the dynamic interaction between HDAC8 and various ligands as well as conformational interconversions during the catalytic reaction. Statistical analysis identified key kinetic parameters, demonstrating that the enzymatic activity is highly sensitive to both minor variations in the ligand structures and small synthetic molecules.
authors.affiliation: Department of Physics, North Dakota State University, Fargo, North Dakota 58108, USA. yongki.choi@ndsu.edu.
authors.forename: Seungyong
authors.initials: S
authors.lastname: You
authors.affiliation: Department of Physics, North Dakota State University, Fargo, North Dakota 58108, USA. yongki.choi@ndsu.edu.
authors.forename: James
authors.initials: J
authors.lastname: Froberg
authors.affiliation: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58108, USA.
authors.forename: Junru
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, North Dakota 58108, USA.
authors.forename: Manas
authors.initials: M
authors.lastname: Haldar
authors.affiliation: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58108, USA.
authors.forename: Abbas
authors.initials: A
authors.lastname: Sedigh
authors.affiliation: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, North Dakota 58108, USA.
authors.forename: Sanku
authors.initials: S
authors.lastname: Mallik
authors.affiliation: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58108, USA.
authors.forename: D K
authors.initials: DK
authors.lastname: Srivastava
authors.affiliation: Department of Physics, North Dakota State University, Fargo, North Dakota 58108, USA. yongki.choi@ndsu.edu.
authors.forename: Yongki
authors.identifier: http://orcid.org/0000-0001-8890-8344
authors.initials: Y
authors.lastname: Choi
mesh_terms: D004553:Electric Conductivity; D004867:Equipment Design; D056572:Histone Deacetylase Inhibitors / Q000737:chemistry; D006655:Histone Deacetylases / Q000737:chemistry*; D006801:Humans; D006877:Hydroxamic Acids / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D036103:Nanotechnology / Q000295:instrumentation*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011487:Protein Conformation; D012097:Repressor Proteins / Q000737:chemistry*; D000077337:Vorinostat
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D008024:Ligands; D037742:Nanotubes, Carbon; D012097:Repressor Proteins; D000077337:Vorinostat; C409470:HDAC8 protein, human; D006655:Histone Deacetylases