title: Construction of recombinant Yarrowia lipolytica and its application in bio-transformation of lignocellulose.
abstract: Lignocellulose is a polysaccharide and an abundant biomass resource that widely exists in grains, beans, rice, and their by-products. Over 10 million tons of lignocellulose resources and processing products are produced every year in China. Three recombinant Y. lipolytica strains with cellulase (β-glucosidase, endoglucanase and cellobiohydrolase) were constructed. The enzymatic activities of these enzymes were 14.181 U/mL, 16.307 U/mL, and 17.391 U/mL, respectively. The whole cell cellulases were used for a stover bio-transformation. The celluloses in the stover were partly degraded by the cellulases, and the degradation products were transformed into single cell protein (SCP) by the Y. lipolytica cells. After 15 d of fermentation with the whole cell cellulases, the protein content of the maize stover and the rice straw reached 16.23% and 14.75%, which increased by 168.26% and 161.52% compared with the control, respectively. This study provides a new stage for the efficient utilization of stover in the feed industry.
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.|b College of Resources and Environmental Science, Hubei University , Wuhan , China.
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Song
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Yang
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.
authors.forename: Ding-Kang
authors.identifier: http://orcid.org/0000-0003-1169-0775
authors.initials: DK
authors.lastname: Liu
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Xu
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Xiao
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.
authors.forename: Zi-Lu
authors.initials: ZL
authors.lastname: Liu
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.
authors.forename: Wu-Cheng
authors.initials: WC
authors.lastname: Xia
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Wang
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.|b College of Resources and Environmental Science, Hubei University , Wuhan , China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: a Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University , Wuhan , China.|c Hubei Key Laboratory of Industrial Biotechnology , College of Life Science, Hubei University , Wuhan , China.
authors.forename: Zheng-Bing
authors.initials: ZB
authors.lastname: Jiang
mesh_terms: D001711:Biotransformation / Q000235:genetics; D044602:Cellulases / Q000235:genetics*; D024861:Genetic Enhancement / Q000379:methods; D008031:Lignin / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D012275:Oryza / Q000382:microbiology*; D035261:Plant Components, Aerial / Q000382:microbiology; D011995:Recombination, Genetic / Q000235:genetics*; D025062:Yarrowia / Q000502:physiology*
chemical_list: C036909:lignocellulose; D008031:Lignin; D044602:Cellulases
keywords: Yarrowia lipolytica; cellulosic feed; lignocellulose; microbial fermentation; single cell protein