title: Long Noncoding RNA PVT1 as a Novel Diagnostic Biomarker and Therapeutic Target for Melanoma.
abstract: Accumulating evidences indicated that plasmacytoma variant translocation 1 (PVT1) plays vital roles in several cancers. However, the expression, functions, and clinical values of PVT1 in melanoma are still unknown. In this study we measured the expression of PVT1 in clinical tissues and serum samples and explored the diagnostic value of PVT1 for melanoma and the effects of PVT1 on melanoma cell proliferation, cell cycle, and migration. Our results, combined with publicly available PVT1 expression data, revealed that PVT1 is upregulated in melanoma tissues compared with nonneoplastic nevi tissues. Serum PVT1 level is significantly increased in melanoma patients compared with age and gender-matched nonmelanoma controls with melanocytic nevus. Receiver operating characteristic curve analyses revealed that serum PVT1 level could sensitively discriminate melanoma patients from controls. Furthermore, serum PVT1 level indicted melanoma dynamics. Functional experiments showed that overexpression of PVT1 promotes melanoma cells proliferation, cell cycle progression, and migration, while depletion of PVT1 significantly inhibits melanoma cells proliferation, cell cycle progression, and migration. Collectively, our results indicate that PVT1 functions as an oncogene in melanoma and could be a potential diagnostic biomarker and therapeutic target for melanoma.
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Xiangjun
authors.identifier: 0000-0003-1972-0828
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Guozhen
authors.identifier: 0000-0002-5354-8227
authors.initials: G
authors.lastname: Gao
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Intensive Care Unit, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Dexiong
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Weijing
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of General Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Dong
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D009857:Oncogenes; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; C521123:PVT1 long-non-coding RNA, human; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm