title: Exogenous gene integration mediated by genome editing technologies in zebrafish.
abstract: Genome editing technologies, such as transcription activator-like effector nuclease (TALEN) and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, can induce DNA double-strand breaks (DSBs) at the targeted genomic locus, leading to frameshift-mediated gene disruption in the process of DSB repair. Recently, the technology-induced DSBs followed by DSB repairs are applied to integrate exogenous genes into the targeted genomic locus in various model organisms. In addition to a conventional knock-in technology mediated by homology-directed repair (HDR), novel knock-in technologies using refined donor vectors have also been developed with the genome editing technologies based on other DSB repair mechanisms, including non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ). Therefore, the improved knock-in technologies would contribute to freely modify the genome of model organisms.
authors.affiliation: a Laboratory for Developmental Biology, Center for Medical Education and Sciences, Graduate School of Medical Science , University of Yamanashi , Chuo , Yamanashi , Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Morita
authors.affiliation: a Laboratory for Developmental Biology, Center for Medical Education and Sciences, Graduate School of Medical Science , University of Yamanashi , Chuo , Yamanashi , Japan.
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Taimatsu
authors.affiliation: a Laboratory for Developmental Biology, Center for Medical Education and Sciences, Graduate School of Medical Science , University of Yamanashi , Chuo , Yamanashi , Japan.
authors.forename: Kanoko
authors.initials: K
authors.lastname: Yanagi
authors.affiliation: a Laboratory for Developmental Biology, Center for Medical Education and Sciences, Graduate School of Medical Science , University of Yamanashi , Chuo , Yamanashi , Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kawahara
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D029961:Zebrafish Proteins
keywords: CRISPR/Cas9; MMEJ; NHEJ; TALEN; genome editing; knock-in; zebrafish