title: A high-performance liquid chromatography-electronic circular dichroism online method for assessing the absolute enantiomeric excess and conversion ratio of asymmetric reactions.
abstract: Asymmetric reactions often need to be evaluated during the synthesis of chiral compounds. However, traditional evaluation methods require the isolation of the individual enantiomer, which is tedious and time-consuming. Thus, it is desirable to develop simple, practical online detection methods. We developed a method based on high-performance liquid chromatography-electronic circular dichroism (HPLC-ECD) that simultaneously analyzes the material conversion ratio and absolute optical purity of each enantiomer. In particular, only a reverse-phase C18 column instead of a chiral column is required in our method because the ECD measurement provides a g-factor that describes the ratio of each enantiomer in the mixtures. We used our method to analyze the asymmetric hydrosilylation of β-enamino esters, and we discussed the advantage, feasibility, and effectiveness of this new methodology.
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Active Substances, Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College &Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Active Substances, Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College &Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Mingchao
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Active Substances, Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College &Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Active Substances, Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College &Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Yin