title: Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA.
abstract: Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC50of FPIA and ic-ELISA based anti-Gent IgY were 7.70±0.6μg/mL and 0.32±0.06μg/mL, respectively. The IC50of FPIA and ic-ELISA based anti-Kana IgY were 7.97±0.9μg/mL and 0.15±0.01μg/mL. The limits of detection (LOD, IC10) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007μg/mL, respectively. The LOD for ic-ELISA were both 0.001μg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100 Shaanxi, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100 Shaanxi, China.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia; Federal Research Center «Fundamentals of Biotechnology» of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.affiliation: College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
authors.forename: Omar
authors.initials: O
authors.lastname: Yakup
authors.affiliation: College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100 Shaanxi, China; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China. Electronic address: zhang.xy@nwsuaf.edu.cn.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D000906:Antibodies / Q000032:analysis; D002417:Cattle; D002645:Chickens; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005839:Gentamicins / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D007136:Immunoglobulins / Q000032:analysis* / Q000737:chemistry; D007612:Kanamycin / Q000032:analysis*; D008460:Meat / Q000032:analysis; D008892:Milk / Q000737:chemistry; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D005839:Gentamicins; C016512:IgY; D007136:Immunoglobulins; D007612:Kanamycin
keywords: Fluorescence polarization immunoassay (FPIA); Gentamicin; Immunoglobulin Y (IgY); Indirect-competitive ELISA (ic-ELISA); Kanamycin