title: Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification.
abstract: Vascular calcification is commonly seen in elderly people, though it can also appear in middle-aged subjects affected by premature vascular aging. The aim of this work is to test the involvement of microvesicles (MVs) produced by senescent endothelial cells (EC) and from plasma of elderly people in vascular calcification. The present work shows that MVs produced by senescent cultured ECs, plus those found in the plasma of elderly subjects, promote calcification in vascular smooth muscle cells. Only MVs from senescent ECs, and from elderly subjects' plasma, induced calcification. This ability correlated with these types of MVs' carriage of: a) increased quantities of annexins (which might act as nucleation sites for calcification), b) increased quantities of bone-morphogenic protein, and c) larger Ca contents. The MVs of senescent, cultured ECs, and those present in the plasma of elderly subjects, promote vascular calcification. The present results provide mechanistic insights into the observed increase in vascular calcification-related diseases in the elderly, and in younger patients with premature vascular aging, paving the way towards novel therapeutic strategies.
authors.affiliation: Departamento de Biología de Sistemas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.|These authors contributed equally to this paper.
authors.forename: Matilde
authors.initials: M
authors.lastname: Alique
authors.affiliation: Departamento de Biología de Sistemas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.|These authors contributed equally to this paper.
authors.forename: María Piedad
authors.initials: MP
authors.lastname: Ruíz-Torres
authors.affiliation: Departamento de Biomedicina y Biotecnología, Facultad de Biología, Química y Ciencias Ambientales, Universidad de Alcalá. Alcalá de Henares, Madrid, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Bodega
authors.affiliation: Unidad de Anestesia, Hospital Universitario Reina Sofía/Universidad de Córdoba, Córdoba, Andalucía, Spain.|Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Hospital Universitario Reina Sofía/Universidad de Córdoba, Córdoba, Andalucía, Spain.
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Noci
authors.affiliation: Departamento de Biología de Sistemas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Troyano
authors.affiliation: Departamento de Biología de Sistemas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Bohórquez
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Hospital Universitario Reina Sofía/Universidad de Córdoba, Córdoba, Andalucía, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Luna
authors.affiliation: Departamento de Química Orgánica, Universidad de Córdoba, Edificio Marie Curie (C-3), Carretera Nacional IV-A, Km 396, E14014, Córdoba, Andalucía, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Luque
authors.affiliation: Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Hospital Universitario Reina Sofía/Universidad de Córdoba, Córdoba, Andalucía, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Carmona
authors.affiliation: Departamento de Fisiología Animal (II), Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain.|Institute of Investigation, Hospital 12 de Octubre, Madrid, Spain.|These senior authors contributed equally to this paper.
authors.forename: Julia
authors.initials: J
authors.lastname: Carracedo
authors.affiliation: Departamento de Biología de Sistemas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.|These senior authors contributed equally to this paper.
authors.forename: Rafael
authors.initials: R
authors.lastname: Ramírez
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000473:pathology*; D002118:Calcium / Q000378:metabolism*; D055252:Cell-Derived Microparticles / Q000473:pathology*; D002478:Cells, Cultured; D016922:Cellular Senescence; D042783:Endothelial Cells / Q000473:pathology*; D006801:Humans; D061205:Vascular Calcification / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
chemical_list: D002118:Calcium
keywords: aging; endothelial cells; microvesicles; senescence; vascular calcification; vascular smooth muscle cells