title: Examining H2O2Production in Arabidopsis Leaves Upon Challenge by Cytokinin.
abstract: Plant cells respond to stresses and exogenous environmental stimuli. Production of altered levels of reactive oxygen species (ROS) is one of a typical cellular responses against such stimuli. These responses are detectable through various techniques including luminol-based bioassays. Luminol-based bioassays provide an excellent opportunity for detection of ROS in plant leaf tissue when challenged with some exogenous stimuli like phytohormones, cytokinins, auxins, ABA, etc. The luminol-based protocol in point aims at measuring changes in the H2O2levels of Arabidopsis leaf discs when exposed to phytohormone cytokinins. However, utility of the luminol-based ROS determination protocol is quite wide. We specifically optimized this protocol to quantify the effect of cytokinins on ROS production by Arabidopsis leaves.
authors.affiliation: Department of Biotechnology, Balochistan University of IT, Engineering and Management Sciences, 87100, Quetta, Pakistan.
authors.forename: Shahjahan Shabbir
authors.initials: SS
authors.lastname: Ahmed
authors.affiliation: Department of Environmental Science, Islamic International University, Islamabad, Sector H-10, Islamabad, Pakistan.
authors.forename: Ihsan
authors.initials: I
authors.lastname: Ullah
authors.affiliation: Department of Biotechnology, Balochistan University of IT, Engineering and Management Sciences, 87100, Quetta, Pakistan.
authors.forename: Shazia
authors.initials: S
authors.lastname: Irfan
authors.affiliation: Department of Biotechnology, Balochistan University of IT, Engineering and Management Sciences, 87100, Quetta, Pakistan. nazeer.ahmed@buitms.edu.pk.
authors.forename: Nazeer
authors.initials: N
authors.lastname: Ahmed
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D003583:Cytokinins / Q000378:metabolism* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D003583:Cytokinins; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Cytokinin; H2O2; Luminol; Reactive oxygen species; Scintillation counter; Signaling