title: Dammarane-type saponins from leaves of Ziziphus spina-christi.
abstract: Phytochemical profiling of Ziziphus spina-christi leaves led to the characterization of 10 dammarane-type saponins and 12 phenolic compounds. Isolation was achieved by gel chromatography on Sephadex LH20, open column chromatography on silica gel, and semi-preparative HPLC with PDA and ELSD detectors. Structural characterization was performed by extensive 1D and 2D NMR, mass spectrometry, and by GC-MS of sugar derivatives. A biosynthetic pathway leading to three previously undescribed sapogenins is proposed. The saponin profiles in Z. spina-christi leaves of four different origins were compared by means of HPLC-ESIMS.
authors.affiliation: Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056, Basel, Switzerland. Electronic address: alen.bozicevic@unibas.ch.
authors.forename: Alen
authors.initials: A
authors.lastname: Bozicevic
authors.affiliation: Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056, Basel, Switzerland. Electronic address: maria.demieri@unibas.ch.
authors.forename: Maria
authors.initials: M
authors.lastname: De Mieri
authors.affiliation: Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056, Basel, Switzerland. Electronic address: angi.dibenedetto@stud.unibas.ch.
authors.forename: Angela
authors.initials: A
authors.lastname: Di Benedetto
authors.affiliation: Mibelle Biochemistry, Mibelle AG, 5033, Buchs, Switzerland.
authors.forename: Frank
authors.initials: F
authors.lastname: Gafner
authors.affiliation: Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056, Basel, Switzerland. Electronic address: matthias.hamburger@unibas.ch.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification; D031957:Ziziphus / Q000737:chemistry*; D000097669:Dammaranes
chemical_list: D012503:Saponins; D014315:Triterpenes; D000097669:Dammaranes
keywords: Dammarane saponins; LC-MS; Lotogenin; Rhamnaceae; Ziziphus spina-christi