title: miR-101-3p Suppresses HOX Transcript Antisense RNA (HOTAIR)-Induced Proliferation and Invasion Through Directly Targeting SRF in Gastric Carcinoma Cells.
abstract: miR-101-3p has been identified as a tumor suppressor in several cancers, but its exact role in gastric adenocarcinoma is still largely unknown. In this study, we found that, compared with the RGM-1 human normal gastric epithelial cells, miR-101-3p was significantly downregulated in all six human gastric adenocarcinoma cell lines, including BGC-823, MNK-45, MGC-803, SGC-7901, AGS, and HGC-27. Overexpression of miR-101-3p suppressed both the proliferation and invasion of AGS gastric adenocarcinoma cells, and knockdown of miR-101-3p displayed the opposite effect. In addition, miR-101-3p could directly target and suppress the expression of the serum response factor (SRF) gene, which is a transcription factor of HOTAIR, a well-characterized tumor promoter lncRNA. miR-101-3p negatively regulated SRF-mediated transcription of HOTAIR. Moreover, silencing of either SRF or HOTAIR could counteract the promotion of gastric adenocarcinoma cell proliferation and invasion by miR-101-3p inhibition. Our findings indicate that miR-101-3p suppresses HOTAIR-induced proliferation and invasion through directly targeting SRF in gastric carcinoma cells.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Wu
authors.forename: Che
authors.initials: C
authors.lastname: Chen
authors.forename: Jiayun
authors.initials: J
authors.lastname: Liu
authors.forename: Guannan
authors.initials: G
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhai
authors.forename: Fukun
authors.initials: F
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D026362:Serum Response Factor / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection
chemical_list: C561566:HOTAIR long untranslated RNA, human; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C476858:SRF protein, human; D026362:Serum Response Factor