title: Induction of Low-Level Hydrogen Peroxide Generation by Unbleached Cotton Nonwovens as Potential Wound Dressing Materials.
abstract: Greige cotton is an intact plant fiber. The cuticle and primary cell wall near the outer surface of the cotton fiber contains pectin, peroxidases, superoxide dismutase (SOD), and trace metals, which are associated with hydrogen peroxide (H₂O₂) generation during cotton fiber development. Traditionally, the processing of cotton into gauze involves scouring and bleaching processes that remove the components in the cuticle and primary cell wall. The use of unbleached, greige cotton fibers in dressings, has been relatively unexplored. We have recently determined that greige cotton can generate low levels of H₂O₂ (5-50 micromolar). Because this may provide advantages for the use of greige cotton-based wound dressings, we have begun to examine this in more detail. Both brown and white cotton varieties were examined in this study. Brown cotton was found to have a relatively higher hydrogen peroxide generation and demonstrated different capacities for H₂O₂ generation, varying from 1 to 35 micromolar. The H₂O₂ generation capacities of white and brown nonwoven greige cottons were also examined at different process stages with varying chronology and source parameters, from field to nonwoven fiber. The primary cell wall of nonwoven brown cotton appeared very intact, as observed by transmission electron microscopy, and possessed higher pectin levels. The levels of pectin, SOD, and polyphenolics, correlated with H₂O₂ generation.
authors.affiliation: United States Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70124, USA. vince.edwards@ars.usda.gov.
authors.forename: J Vincent
authors.initials: JV
authors.lastname: Edwards
authors.affiliation: United States Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70124, USA. nicolette.prevost@ars.usda.gov.
authors.forename: Nicolette T
authors.initials: NT
authors.lastname: Prevost
authors.affiliation: United States Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70124, USA. Sunghyun.nam@ars.usda.gov.
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Nam
authors.affiliation: United States Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70124, USA. doug.hinchliffe@ars.usda.gov.
authors.forename: Doug
authors.initials: D
authors.lastname: Hinchliffe
authors.affiliation: United States Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70124, USA. brian.condon@ars.usda.gov.
authors.forename: Brian
authors.initials: B
authors.lastname: Condon
authors.affiliation: Plastic and Reconstructive Surgery, Virginia Commonwealth University, Richmond, VA 23111, USA. dorne.yager@vcuhealth.org.
authors.forename: Dorne
authors.initials: D
authors.lastname: Yager
keywords: chronic wounds; cotton; hydrogen peroxide; wound dressings; wound healing