title: Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria with tolerance to hypoxic environments.
abstract: Hypoxic conditions are considerably different from aerobic and anaerobic conditions, and they are widely distributed in natural environments. Many pollutants, including polycyclic aromatic hydrocarbons (PAHs), tend to accumulate in hypoxic environments. However, PAH biodegradation under hypoxic conditions is poorly understood compared with that under obligate aerobic and obligate anaerobic conditions. In the present study, PAH-degrading bacteria were enriched, and their biodegradation rates were tested using a hypoxic station with an 8% oxygen concentration. PAH-degrading bacteria collected from sediments in low-oxygen environments were enriched using phenanthrene (Phe) or pyrene (Pyr) as the sole carbon and energy source. Individual bacterial colonies showing the ability to degrade Phe or Pyr were isolated and identified by 16S rDNA gene sequencing. Morphological and physiological characterizations of the isolated bacterial colonies were performed. The isolated bacteria were observed by scanning electron microscopy (SEM) and were identified as Pseudomonas sp., Klebsiella sp., Bacillus sp., and Comamonas sp. Phylogenetic tree of the isolated PAH-degrading bacteria was also constructed. The biodegradation ability of these bacteria was tested at an initial Phe or Pyr concentration of 50 mg L-1. The biodegradation kinetics were best fit by a first-order rate model and presented regression coefficients (r2) that varied from 0.7728 to 0.9725 (P < 0.05). The half-lives of the PAHs varied from 2.99 to 3.65 d for Phe and increased to 60.3-82.5 d for Pyr. These half-lives were much shorter than those observed under anaerobic conditions but were similar to those observed under aerobic conditions.
authors.affiliation: a State Key Laboratory of Environmental Criteria and Risk Assessment , Chinese Research Academy of Environmental Sciences , Beijing , China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Environmental Criteria and Risk Assessment , Chinese Research Academy of Environmental Sciences , Beijing , China.
authors.forename: Chun
authors.initials: C
authors.lastname: Ye
authors.affiliation: a State Key Laboratory of Environmental Criteria and Risk Assessment , Chinese Research Academy of Environmental Sciences , Beijing , China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Hou
authors.affiliation: a State Key Laboratory of Environmental Criteria and Risk Assessment , Chinese Research Academy of Environmental Sciences , Beijing , China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Chen
authors.affiliation: c Zhejiang Provincial Key Lab for Subtropical Water Environment and Marine Biological Resources Protection, School of Life and Environmental Science, Wenzhou University , Zhejiang , China.
authors.forename: Xiang-Yong
authors.initials: XY
authors.lastname: Zheng
authors.affiliation: b School of Water Resource and Environment, China University of Geosciences , Beijing , China.
authors.forename: Xu-Yi
authors.initials: XY
authors.lastname: Cai
mesh_terms: D000693:Anaerobiosis; D001407:Bacillus / Q000302:isolation & purification / Q000502:physiology; D001673:Biodegradation, Environmental; D002681:China; D020585:Comamonas / Q000302:isolation & purification / Q000502:physiology; D004275:DNA, Ribosomal / Q000235:genetics; D019015:Geologic Sediments / Q000032:analysis* / Q000382:microbiology*; D007709:Klebsiella / Q000302:isolation & purification / Q000502:physiology; D010100:Oxygen / Q000032:analysis; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D011549:Pseudomonas / Q000302:isolation & purification / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D004275:DNA, Ribosomal; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D010100:Oxygen
keywords: Bacteria; biodegradation; hypoxic environment; polycyclic aromatic hydrocarbons (PAHs)