title: Assessment of cathepsin mRNA expression and enzymatic activity during early embryonic development in the yellowtail kingfish Seriola lalandi.
abstract: In pelagic species such as Seriola lalandi, survival of both the eggs and embryos depends on yolk processing during oocyte maturation and embryo development. The main enzymes involved in these processes are the cathepsins, which are essential for the hydration process, acquiring buoyancy and nutrition of the embryo before hatching. This study aimed to investigate the mRNA expression profiles of cathepsins B, D and L (catb, catd and catl) and the activity of these enzymes during early development in S. lalandi. We included previtellogenic oocytes (PO). All three enzymes were highly expressed in PO, but the expression was reduced throughout development. Between PO and recently spawned eggs (E1) the transcript to catb and catd decreased, unlike catl. Cathepsin B activity, showed stable levels between PO until blastula stage (E4). High activities levels of cathepsins D and L were observed in E1 in comparison with later developmental stages. Cathepsin L activity remained constant until E1, consistent with observations in other pelagic spawners, where its participation in a second protolithic cleavage of the yolk proteins, has been proposed for this enzyme. Their profiles of both mRNA expression and enzymatic activity indicate the importance of these enzymes during early development and suggest different roles in egg yolk processing for the hydration process and nutrition in early embryos in this species.
authors.affiliation: FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile; Animal Reproduction Laboratory, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile.
authors.forename: Jaime
authors.initials: J
authors.lastname: Palomino
authors.affiliation: FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile; Animal Reproduction Laboratory, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile.
authors.forename: Giannina
authors.initials: G
authors.lastname: Herrera
authors.affiliation: Animal Reproduction Laboratory, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile.
authors.forename: Jorge
authors.initials: J
authors.lastname: Torres-Fuentes
authors.affiliation: FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile.
authors.forename: Phillip
authors.initials: P
authors.lastname: Dettleff
authors.affiliation: FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile.
authors.forename: Alok
authors.initials: A
authors.lastname: Patel
authors.affiliation: FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Avda. Santa Rosa 11735, Casilla 2 Correo 15, Santiago, Chile. Electronic address: vmartine@uchile.cl.
authors.forename: Víctor
authors.initials: V
authors.lastname: Martínez
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D010473:Perciformes / Q000196:embryology*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D002403:Cathepsins
keywords: Cathepsins; Embryo development; Enzymatic activity; RT-qPCR; Seriola