title: Insecticide susceptibility status and major detoxifying enzymes' activity in Aedes albopictus (Skuse), vector of dengue and chikungunya in Northern part of West Bengal, India.
abstract: Mosquitoes belonging to Aedes genus, Aedes aegypti and Aedes albopictus transmit many globally important arboviruses including Dengue (DENV) and Chikungunya (CHIKV). Vector control with the use of insecticide remains the suitable method of choice to stop the transmission of these diseases. However, vector control throughout the world is failing to achieve its target results because of the worldwide development of insecticide resistance in mosquitoes. To assess the insecticide susceptibility status of Aedes albopictus from northern part of West Bengal, the susceptibility of eight different Aedes albopictus populations were tested against a commonly used larvicide (temephos) and some adulticides (malathion, deltamethrin and lambda cyhalothrin) along with the major insecticide detoxifying enzymes' activity in them. Through this study, it was revealed that most of the populations were found susceptible to temephos except Nagrakata (NGK) and Siliguri (SLG), which showed both a higher resistance ratio (RR99) and a lower susceptibility, thereby reflecting the development of resistance against temephos in them. However, all tested adulticides caused 100% mortality in all the population implying their potency in control of this mosquito in this region of India. Through the study of carboxylesterase activity, it was revealed that the NGK population showed a 9.6 fold higher level of activity than susceptible population. The same population also showed a lower level of susceptibility and a higher resistance ratio (RR99), indicating a clear correlation between susceptibility to temephos and carboxylesterase enzymes' activity in this population. This preliminary data reflects that the NGK population is showing a trend towards resistance development and with time, there is possibility that this resistance phenomenon will spread to other populations. With the recurrence of dengue and chikungunya, this data on insecticide susceptibility status of Aedes albopictus could help the authorities engaged in vector control programmes to formulate effective measures against this mosquito in this region.
authors.affiliation: Insect Biochemistry and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, P.O. North Bengal University, Siliguri 734013, District-Darjeeling, West Bengal, India.
authors.forename: Minu
authors.initials: M
authors.lastname: Bharati
authors.affiliation: Insect Biochemistry and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, P.O. North Bengal University, Siliguri 734013, District-Darjeeling, West Bengal, India. Electronic address: dhirajsaha.nbu@gmail.com.
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Saha
mesh_terms: D000330:Aedes / Q000201:enzymology*; D000818:Animals; D065632:Chikungunya Fever / Q000635:transmission*; D003715:Dengue / Q000635:transmission*; D004198:Disease Susceptibility; D006801:Humans; D007194:India / Q000453:epidemiology; D007303:Insect Vectors / Q000201:enzymology*; D007305:Insecticide Resistance / Q000187:drug effects*; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D008294:Malathion / Q000494:pharmacology; D009570:Nitriles / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D000002:Temefos / Q000494:pharmacology
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D000002:Temefos; D008294:Malathion; C037304:cyhalothrin
keywords: Aedes albopictus; Dengue; Detoxifying enzymes’ activity; Insecticide susceptibility status; Vector control