title: OSMAC approach leads to new fusarielin metabolites from Fusarium tricinctum.
abstract: Using the OSMAC (One Strain MAny Compounds) approach, the fungal endophyte Fusarium tricinctum was cultivated on fruit and vegetable juice-supplemented solid rice media. This led to an up to 80-fold increase in the accumulation of the new natural product fusarielin J (1), as well as to the induction of two new natural products fusarielin K (2) and fusarielin L (3) and the known derivatives fusarielins A (4) and B (5). Compounds 2-5 were not detected when the fungus was grown on rice media lacking either fruit or vegetable juice. The highest increase in the accumulation of compound 1 was observed in the presence of apple and carrot juice, whereas the stimulating effect was weaker for banana juice. Compound 1 exhibited cytotoxicity against the human ovarian cancer cell line A2780, with an IC50value of 12.5 μM.
authors.affiliation: Institute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany.
authors.forename: Catalina F Pérez
authors.initials: CFP
authors.lastname: Hemphill
authors.affiliation: Institute of Pharmaceutical and Medicinal Chemistry, Heinrich Heine University, Duesseldorf, Germany.
authors.forename: Parichat
authors.initials: P
authors.lastname: Sureechatchaiyan
authors.affiliation: Institute of Pharmaceutical and Medicinal Chemistry, Heinrich Heine University, Duesseldorf, Germany.
authors.forename: Matthias U
authors.initials: MU
authors.lastname: Kassack
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Raha S
authors.initials: RS
authors.lastname: Orfali
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, People's Republic of China.
authors.forename: Wenhan
authors.initials: W
authors.lastname: Lin
authors.affiliation: Institute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany.
authors.forename: Georgios
authors.initials: G
authors.lastname: Daletos
authors.affiliation: Institute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Proksch
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D003470:Culture Media; D046508:Culture Techniques; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D000067030:Fruit and Vegetable Juices; D005670:Fusarium / Q000378:metabolism*; D006801:Humans; D020128:Inhibitory Concentration 50; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012275:Oryza / Q000382:microbiology; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D004852:Epoxy Compounds; D009281:Naphthalenes; C092174:fusarielin A; C092175:fusarielin B; C000620995:fusarielin J; C000620994:fusarielin K; C000620993:fusarielin L