title: Influence of hydrolysis behaviour and microfluidisation on the functionality and structural properties of collagen hydrolysates.
abstract: The functionality and structural properties of pig skin hydrolysates with different degrees of hydrolysis (DH, 10% and 20%) and microfluidisation (120MPa), prepared by pepsin and Alcalase® have been investigated in this study. Extensive hydrolysis can significantly improve the absolute value of the zeta potential and surface hydrophobicity. The particle distribution of hydrolysates decreased with increasing DH. The numbers of free sulfhydryl (SH) and disulfide bonds (SS) were significantly increased with increasing DH (p<0.05). Hydrolysates with a lower DH showed a better emulsifying property than those with a higher DH. Microfluidisation led to the transformation of structural and interfacial properties of the hydrolysates and increased the value of the zeta potential, S0, and gel strength. Microfluidisation results in limited breakage of chemical bonds, the number of SS and SH bonds unchanged in the treatment. These results reflect the functionality and structural properties of collagen-rich pig skin hydrolysates.
authors.affiliation: Sericulture & Agri-food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
authors.forename: Yehui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Sericulture & Agri-food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
authors.forename: Yousheng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Sericulture & Agri-food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Food, Guangzhou City Polytechnic, Guangzhou 510405, China. Electronic address: hlh@gcp.edu.cn.
authors.forename: Lihua
authors.initials: L
authors.lastname: Huang
authors.affiliation: Sericulture & Agri-food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Sericulture & Agri-food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
authors.forename: Jingrong
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000818:Animals; D003094:Collagen; D004655:Emulsions; D057927:Hydrophobic and Hydrophilic Interactions; D044085:Microfluidics; D011492:Protein Hydrolysates / Q000737:chemistry*; D012867:Skin; D013552:Swine
chemical_list: D004655:Emulsions; D011492:Protein Hydrolysates; D003094:Collagen
keywords: Collagen hydrolysate; Degree of hydrolysis; Functionality; Microfluidisation; Pig skin; Structural properties