title: Inhibitory effect of Zanthoxylum bungeanum essential oil (ZBEO) on Escherichia coli and intestinal dysfunction.
abstract: The inhibitory effects of Zanthoxylum bungeanum essential oil (ZBEO) on Escherichia coli (E. coli) in vitro and in vivo were investigated, as well as its function of improvement of intestinal health. The results of in vitro studies, such as minimal inhibitory concentration (MIC) analysis, agar disc diffusion test and growth curve analysis of E. coli, showed that ZBEO had an excellent inhibitory effect on the growth of E. coli, which may be related to the loss of the normal shape of the cell membranes and the leakage of intracellular constituents, on the basis of SEM observation and cell constituents' release assay. ZBEO also had an inhibitory effect on enteritis and intestinal dysfunction induced by infection of E. coli in vivo, and histopathological observation indicated that ZBEO could markedly ameliorate the structural destruction of intestinal tissues, which might be related to its inhibitory effect on the gene expression of inflammatory cytokines (TLR2, TLR4, TNFαand IL-8). In conclusion, ZBEO showed an excellent inhibitory effect on E. coli both in vitro and in vivo, suggesting the potential application of ZBEO as a kind of functional component having the effects of improving intestinal function and health.
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China. leihong56@163.com qiuhuihu@njue.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Hong
authors.forename: Wu
authors.initials: W
authors.lastname: Jing
authors.forename: Wang
authors.initials: W
authors.lastname: Qing
authors.forename: Su
authors.initials: S
authors.lastname: Anxiang
authors.forename: Xue
authors.initials: X
authors.lastname: Mei
authors.forename: Liu
authors.initials: L
authors.lastname: Qin
authors.forename: Hu
authors.initials: H
authors.lastname: Qiuhui
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000503:physiopathology; D051379:Mice; D009822:Oils, Volatile / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D016209:Interleukin-8; D009822:Oils, Volatile; D010936:Plant Extracts; D051195:Toll-Like Receptor 2