title: Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes.
abstract: Vibrio parahaemolyticus, found frequently in oysters, is the most prevalent gastroenteritis-causing pathogen in Korea and in several other Asian countries. This study monitored changes in the environmental parameters and occurrence of V. parahaemolyticus in oyster aquaculture sites. Of the 44 presumed V. parahaemolyticus isolates obtained, when tested against 16 antibiotics, 90.9, 86.4, and 75.0% of the 44 isolates exhibited resistance to vancomycin, ampicillin, and streptomycin, respectively. PCR analysis for the presence of the toxR gene confirmed 31 of the 44 isolates as being positive V. parahaemolyticus strains. The toxR positive isolates were tested for the presence of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) virulence genes. Only 9.1% toxR positive isolate exhibit the trh gene and none of the isolates were tested positive for tdh. The occurrence of multi drug resistance strains in the environment could be an indication of excessive usage of antibiotics in agriculture and aquaculture fields.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, Republic of Korea.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Kang
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, Republic of Korea.
authors.forename: YuJin
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, Republic of Korea.
authors.forename: SeokCheol
authors.initials: S
authors.lastname: Jang
authors.affiliation: West sea Fisheries Institute, National Institute of Fisheries Sciencet (NIFS), Incheon, Republic of Korea.
authors.forename: HongSik
authors.initials: H
authors.lastname: Yu
authors.affiliation: West sea Fisheries Institute, National Institute of Fisheries Sciencet (NIFS), Incheon, Republic of Korea.
authors.forename: SuKyung
authors.initials: S
authors.lastname: Kim
authors.affiliation: West sea Fisheries Institute, National Institute of Fisheries Sciencet (NIFS), Incheon, Republic of Korea.
authors.forename: Sera
authors.initials: S
authors.lastname: An
authors.affiliation: Food Safety and Processing Research Division, National Institute of Fisheries Science (NIFS), Busan, Republic of Korea.
authors.forename: Kunbawui
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, Republic of Korea. Electronic address: sjaeseon@inha.ac.kr.
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: So
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017756:Aquaculture; D001208:Asia; D001427:Bacterial Toxins / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D012758:Shellfish; D014736:Vibrio parahaemolyticus / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D037521:Virulence Factors; C068489:thermostable direct hemolysin
keywords: Occurrence; Oyster; Vibrio parahaemolyticus; toxR; trh