title: Reflectometric Interference Spectroscopy.
abstract: Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance. The advantages of direct optical detection depend on a sophisticated surface chemistry resulting negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.
authors.affiliation: Institute of Physical and Theoretical Chemistry, University of Tuebingen, Auf der Morgenstelle 18, 72076, Tuebingen, Germany. guenther.proll@ipc.uni-tuebingen.de.|Biametrics GmbH, Waldhaeuser Str. 64, 72076, Tuebingen, Germany. guenther.proll@ipc.uni-tuebingen.de.
authors.forename: Guenther
authors.initials: G
authors.lastname: Proll
authors.affiliation: Biametrics GmbH, Waldhaeuser Str. 64, 72076, Tuebingen, Germany.
authors.forename: Goran
authors.initials: G
authors.lastname: Markovic
authors.affiliation: Biametrics GmbH, Waldhaeuser Str. 64, 72076, Tuebingen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Fechner
authors.affiliation: Institute of Physical and Theoretical Chemistry, University of Tuebingen, Auf der Morgenstelle 18, 72076, Tuebingen, Germany.|Biametrics GmbH, Waldhaeuser Str. 64, 72076, Tuebingen, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Proell
authors.affiliation: Institute of Physical and Theoretical Chemistry, University of Tuebingen, Auf der Morgenstelle 18, 72076, Tuebingen, Germany.
authors.forename: Guenter
authors.initials: G
authors.lastname: Gauglitz
mesh_terms: D000906:Antibodies / Q000276:immunology; D000915:Antibody Affinity / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001704:Biopolymers; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005336:Fiber Optic Technology / Q000295:instrumentation; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding / Q000276:immunology; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods
chemical_list: D000906:Antibodies; D000941:Antigens; D001704:Biopolymers; D008024:Ligands
keywords: Label-free optical biosensor; Reflectometric interference spectroscopy (RIfS)