title: Prognosis and Progression of ESCC Patients with Perineural Invasion.
abstract: Perineural invasion (PNI) has been recognized as a poor prognostic factor in several malignancies, but the definition and pathogenesis of PNI in esophageal squamous cell carcinoma (ESCC) remains to be defined. PNI was evaluated by H&E staining and S100 immunohistochemistry. The predictive value of PNI in the prognosis of ESCC patients was analyzed. PNI was evaluated in vitro and in vivo. A total of 54 specimens (17.88%) were defined as PNI-a and 99 specimens (32.78%) as PNI-b. S100 staining was superior to H&E staining for PNI detection (50.66% vs 27.15%, P < 0.001, κ = 0.506). Tumor depth (P = 0.001), tumor stage (P = 0.010), and vascular invasion (P < 0.001) were significantly associated with PNI. PIN-a and PNI-b had significant lower disease free survival (DFS) and disease specific survival (DSS) than PNI-0 patients, and the prognosis of PNI-b patients was significantly worse than PNI-a patients for DFS (P = 0.009). PNI was an independent predictor for DFS and DSS in ESCC as evaluated by univariate and multivariate analyses. ESCC cells could metastasize along the nerve in vitro and in vivo, and PNI was a dynamic process. S100 staining significantly improved the accuracy of PNI detection. PNI was associated with local recurrence and poor prognosis of ESCC patients.
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Shushang
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Gaozan
authors.initials: G
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Shuao
authors.initials: S
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Guocai
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Lian
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Man
authors.initials: M
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Cancer Biology, Division of Digestive Surgery, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010525:Peripheral Nerves / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D017207:Rats, Sprague-Dawley; D012189:Retrospective Studies; D009418:S100 Proteins / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D009418:S100 Proteins