title: CASC2c as an unfavorable prognosis factor interacts with miR-101 to mediate astrocytoma tumorigenesis.
abstract: miR-101 has been suggested as a tumor suppressor, but the promoter methylation and loss of heterozygosity didn't contribute to its low expression in astrocytoma. We investigated the role of a new long non-coding RNA CASC2c binding with miR-101. High CASC2c was positively correlated with astrocytoma progression, and an unfavorable prognosis factor for patients. Knockdown CASC2c inhibited proliferation and tumorgenesis. Overexpression of CASC2c promotes the malignant characteristic of astrocytoma cells.CASC2c directly bound miR-101 and mediated pre-miR-101 processing into mature miR-101, and functions as a competitor of miR-101 target genes such as CPEB1. Patients who possessed both low CASC2c and high miR-101 had a longer survival than those of low CASC2c alone or high miR-101 alone. In summary, CASC2c plays the onco-RNA role in the tumorgenesis of astrocytoma by acting as a decoy miR-101 sponge. Combination of low expression of CASC2c and high expression of miR-101 has an important referential significance to evaluate the prognosis of patients.
authors.affiliation: Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya Medical School, Central South University, Changsha 410013, China.|Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha 410078, China.|Key Laboratory of Carcinogenesis, Ministry of Health, Changsha 410078, China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya Medical School, Central South University, Changsha 410013, China.|Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha 410078, China.|Key Laboratory of Carcinogenesis, Ministry of Health, Changsha 410078, China.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Second Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: She
authors.affiliation: Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha 410078, China.|Key Laboratory of Carcinogenesis, Ministry of Health, Changsha 410078, China.
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Tu
authors.affiliation: Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
authors.forename: Xiping
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha 410078, China.|Key Laboratory of Carcinogenesis, Ministry of Health, Changsha 410078, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha 410078, China.|Key Laboratory of Carcinogenesis, Ministry of Health, Changsha 410078, China.
authors.forename: Peiyao
authors.initials: P
authors.lastname: Li
authors.affiliation: Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya Medical School, Central South University, Changsha 410013, China.|Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha 410078, China.|Key Laboratory of Carcinogenesis, Ministry of Health, Changsha 410078, China.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
authors.affiliation: Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha 410078, China.|Key Laboratory of Carcinogenesis, Ministry of Health, Changsha 410078, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology*; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002843:Chromatin / Q000235:genetics; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D002843:Chromatin; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D025521:Tumor Suppressor Proteins; C484713:long non-coding RNA CASC2, human