title: Influence of designer self-assembling nanofiber scaffolds containing anti-cancer peptide motif on hepatoma carcinoma cells.
abstract: Nanotechnology provides a novel scope for cancer therapeutics. Nanoscale platforms could influence the survival, adhesion, and migration of cancer cells through altering the tumor microenvironments. We report here the development of an anti-tumor material designed specifically for cancer therapy. We synthesized a 48-amino acid peptide RADA-KLA, which could self-assemble into nanostructures with antitumor activities. We tested the microstructure of RADA-KLA nanofiber scaffold using transmission electron microscopy (TEM) and accessed rheological properties of the material. Then, we demonstrated the effects of RADA-KLA nanofibers on hepatoma carcinoma cells. We found that RADA-KLA self-assembling peptide scaffold could induce cell death, and inhibit adhesion and migration of hepatoma carcinoma cells. Our results indicate that the designer peptide scaffold has anti-tumor activities and could be used for cancer therapy. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2329-2334, 2017.
authors.affiliation: Institute of Infection and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
authors.forename: Pindong
authors.initials: P
authors.lastname: Li
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Jin
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 JieFang Avenue, Wuhan, 430022, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Peng
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D008113:Liver Neoplasms / Q000188:drug therapy*; D008958:Models, Molecular; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D020100:Hydrogels; D036341:Intercellular Signaling Peptides and Proteins; C535058:KLA peptide; D010455:Peptides
keywords: adhesion; cancer; migration; peptide; self-assemble