title: Engineered yeast with a CO2-fixation pathway to improve the bio-ethanol production from xylose-mixed sugars.
abstract: Bio-ethanol production from lignocellulosic raw materials could serve as a sustainable potential for improving the supply of liquid fuels in face of the food-to-fuel competition and the growing energy demand. Xylose is the second abundant sugar of lignocelluloses hydrolysates, but its commercial-scale conversion to ethanol by fermentation is challenged by incomplete and inefficient utilization of xylose. Here, we use a coupled strategy of simultaneous maltose utilization and in-situ carbon dioxide (CO2) fixation to achieve efficient xylose fermentation by the engineered Saccharomyces cerevisiae. Our results showed that the introduction of CO2as electron acceptor for nicotinamide adenine dinucleotide (NADH) oxidation increased the total ethanol productivity and yield at the expense of simultaneous maltose and xylose utilization. Our achievements present an innovative strategy using CO2to drive and redistribute the central pathways of xylose to desirable products and demonstrate a possible breakthrough in product yield of sugars.
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.|National Energy R&D Center for Biorefinery, Beijing, People's Republic of China.|Beijing Key Laboratory of Bioprocess, Beijing, People's Republic of China.
authors.forename: Yun-Jie
authors.initials: YJ
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.|National Energy R&D Center for Biorefinery, Beijing, People's Republic of China.|Beijing Key Laboratory of Bioprocess, Beijing, People's Republic of China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.|National Energy R&D Center for Biorefinery, Beijing, People's Republic of China.|Beijing Key Laboratory of Bioprocess, Beijing, People's Republic of China.
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Chen
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.|National Energy R&D Center for Biorefinery, Beijing, People's Republic of China.|Beijing Key Laboratory of Bioprocess, Beijing, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.|National Energy R&D Center for Biorefinery, Beijing, People's Republic of China.|Beijing Key Laboratory of Bioprocess, Beijing, People's Republic of China.
authors.forename: Li-Hai
authors.initials: LH
authors.lastname: Fan
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.|National Energy R&D Center for Biorefinery, Beijing, People's Republic of China.|Beijing Key Laboratory of Bioprocess, Beijing, People's Republic of China.
authors.forename: Tian-Wei
authors.initials: TW
authors.lastname: Tan
mesh_terms: D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D008320:Maltose / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D000073893:Sugars / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000073893:Sugars; D009243:NAD; D002245:Carbon Dioxide; D000431:Ethanol; D008320:Maltose; D014994:Xylose