title: Generation of a transgenic cashmere goat using the piggyBac transposition system.
abstract: The development of transgenic technologies in the Cashmere goat (Capra hircus) has the potential to improve the quality of the meat and wool. The piggyBac (PB) transposon system is highly efficient and can be used to transpose specific target genes into the genome. Here, we developed a PB transposon system to produce transgenic Cashmere goat fetal fibroblasts (GFFs) with the enhanced green fluorescent protein (EGFP). We then used the genetically modified GFFs as nuclear donors to generate transgenic embryos by somatic cell nuclear transfer (SCNT). The embryos (n = 40) were implanted into female goats (n = 20). One transgenic kid that expressed EGFP throughout the surface features of its body was born. This result demonstrated the usefulness of PB transposon system in generating transgenic Cashmere goats.
authors.affiliation: College of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou 350002, China; Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fuzhou 350002, China.
authors.forename: Ding-Ping
authors.initials: DP
authors.lastname: Bai
authors.affiliation: College of Animal Science and Technology, Northwest Agricultural and Forestry University, Yangling 712100, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Yang
authors.affiliation: Shaanbei Cashmere Goats Engineering Technology Research Center of Shaanxi Province, Yulin College, Yulin 719000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qu
authors.affiliation: College of Animal Science and Technology, Northwest Agricultural and Forestry University, Yangling 712100, China. Electronic address: chenyulin@nwsuaf.edu.cn.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Chen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D000831:Animals, Newborn / Q000235:genetics; D004247:DNA / Q000032:analysis; D004251:DNA Transposable Elements; D004624:Embryo Transfer; D005260:Female; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts; D005818:Genetic Engineering / Q000379:methods / Q000662:veterinary*; D006041:Goats / Q000235:genetics*; D049452:Green Fluorescent Proteins; D008460:Meat; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011247:Pregnancy; D014162:Transfection / Q000662:veterinary; D014935:Wool
chemical_list: D004251:DNA Transposable Elements; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA
keywords: Cashmere goats; SCNT; Transgenic; Transposition; piggyBac