title: Bio-active polymer implants to adipose tissue as in situ source of reprogramming cells.
abstract: The implants from natural polymer chitosan was developed by sorption of non-viral DNA vector. These assemblies were shown to be biocompatible and able to transfect the surrounding cells with reprogramming factors. Transgenic rats from the strain allowing visualization of Nanog-positive stem cells received these implants into their adipose deposits. After 8 days we were able to detect in situ-transfected cells. Transfected cells were positive for YFP fluorescence and transiently expressed the markers correlating with stemness.
authors.forename: Andrey
authors.initials: A
authors.lastname: Mikhailov
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Sugiyama
authors.forename: Saori
authors.initials: S
authors.lastname: Mizuno
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sankai
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D048271:Chitosan / Q000737:chemistry*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D000071317:Nanog Homeobox Protein / Q000235:genetics / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D012313:RNA / Q000378:metabolism; D051381:Rats; D055647:Rats, Transgenic; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014162:Transfection
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D000071317:Nanog Homeobox Protein; D011108:Polymers; C054562:yellow fluorescent protein, Bacteria; D012313:RNA; D048271:Chitosan