title: Effect of Genital Sampling Site on the Detection and Quantification ofUreaplasmaSpecies with Quantitative Polymerase Chain Reaction during Pregnancy.
abstract: Objective. This study aimed to compare the qualitative and quantitative reproducibility of quantitative PCR (qPCR) forUreaplasmaspecies(Ureaplasmaspp.) throughout pregnancy and according to the genital sampling site.Study Design. Between 5 and 14 weeks of gestation (T1), vaginal, fornix, and two cervical samples were taken. Sampling was repeated during the 2nd (T2) and 3rd (T3) trimester in randomly selected T1 positive and negative women. Qualitative and quantitative reproducibility were evaluated using, respectively, Cohen's kappa (κ) and interclass correlation coefficients (ICC) and repeated measures ANOVA on the log-transformed mean number of DNA copies for each sampling site.Results. During T1, 51/127 women were positive forU. parvumand 8 forU. urealyticum(4 patients for both). Sampling was repeated for 44/55 women at T2 and/or T3; 43 (97.7%) remained positive at the three timepoints.κranged between 0.83 and 0.95 and the ICC for cervical samples was 0.86.Conclusions. Colonization byUreaplasmaspp. seems to be very constant during pregnancy and vaginal samples have the highest detection rate.
authors.affiliation: Department of Obstetrics and Prenatal Medicine, Universitair Ziekenhuis Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium.
authors.forename: Gilles
authors.identifier: 0000-0003-2603-7488
authors.initials: G
authors.lastname: Faron
authors.affiliation: Department of Microbiology, UZ Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium.
authors.forename: Ellen
authors.initials: E
authors.lastname: Vancutsem
authors.affiliation: Department of Microbiology, UZ Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium.
authors.forename: Anne
authors.identifier: 0000-0003-2527-860X
authors.initials: A
authors.lastname: Naessens
authors.affiliation: Department of Biostatistics and Medical Informatics, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium.
authors.forename: Ronald
authors.initials: R
authors.lastname: Buyl
authors.affiliation: Department of Obstetrics and Prenatal Medicine, Universitair Ziekenhuis Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Gucciardo
authors.affiliation: Department of Obstetrics and Prenatal Medicine, Universitair Ziekenhuis Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium.
authors.forename: Walter
authors.initials: W
authors.lastname: Foulon
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005260:Female; D005836:Genitalia, Female / Q000382:microbiology*; D006801:Humans; D058889:Molecular Typing / Q000379:methods / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D011247:Pregnancy; D011295:Prenatal Care / Q000379:methods / Q000592:standards; D011446:Prospective Studies; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D014509:Ureaplasma / Q000235:genetics* / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000175:diagnosis / Q000382:microbiology; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial