title: Studying the Dynamics of TCR Internalization at the Immune Synapse.
abstract: Establishing a stable interaction between a T cell and an antigen presenting cell (APC) involves the formation of an immune synapse (IS). It is through this structure that the T cell can integrate all the signals provided by the APC. The IS also serves as a mechanism for TCR downregulation through internalization. Here, we describe methods for visualizing MHC-engaged T cell receptor (TCR) internalization from the IS in human cell lines and mouse primary T cells by confocal fluorescence microscopy techniques.
authors.affiliation: Cell Biology and Immunology Department, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Calleja
authors.affiliation: Cell Biology and Immunology Department, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Balbino
authors.initials: B
authors.lastname: Alarcón
authors.affiliation: Cell Biology and Immunology Department, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, Spain. oeste@cbm.csic.es.
authors.forename: Clara L
authors.initials: CL
authors.lastname: Oeste
mesh_terms: D000818:Animals; D006801:Humans; D054992:Immunological Synapses / Q000276:immunology*; D019169:Jurkat Cells; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: Antigen presenting cell; Confocal fluorescence microscopy; Immune synapse; MHC-peptide; SMAC; T cell; TCR internalization