title: The intestinal proteome of diabetic and control children is enriched with different microbial and host proteins.
abstract: In this study, the intestinal microbial proteome of children with established type 1 diabetes (T1D) was compared with the proteome of healthy children (Control) with the aim to identify differences in the activity of the intestinal microbiota that not only will contribute to a deeper knowledge of the functionality of the gut in these children but also may provide new approaches to improve the control of the disease. Faecal protein extracts collected from three T1D children (aged 9.3±0.6 years) and three Control children (aged 9.3±1.5 years) were analysed using a combination of 2D gel electrophoresis and spectral counting. The results evidenced markedly differences between the intestinal proteome of T1D children and the Control. The T1D microbial intestinal proteome was enriched with proteins of clostridial cluster XVa and cluster IV and Bacteroides. In contrast, the Control proteome was enriched with bifidobacterial proteins. In both groups, proteins with moonlight function were observed. Human proteins also distinguished the two groups with T1D children depleted in exocrine pancreatic enzymes.
authors.affiliation: Faculdade de Ciências e Tecnologia, Centro de Investigação em Biomedicina, Universidade do Algarve Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Elsa
authors.initials: E
authors.lastname: Pinto
authors.affiliation: Núcleo de Diabetologia, Nutrição e Doenças Metabólicas (NDNDM) do Hospital de Faro, 8000-386 Faro, Portugal.
authors.forename: Marisol
authors.initials: M
authors.lastname: Anselmo
authors.affiliation: Núcleo de Diabetologia, Nutrição e Doenças Metabólicas (NDNDM) do Hospital de Faro, 8000-386 Faro, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Calha
authors.affiliation: Protein and Nucleic Acid Chemistry Laboratory (PNACL), University of Leicester, Leicester LE1 7RH, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bottrill
authors.affiliation: Faculdade de Ciências e Tecnologia, Centro de Investigação em Biomedicina, Universidade do Algarve Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Duarte
authors.affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 7RH, UK.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Andrew
authors.affiliation: Faculdade de Ciências e Tecnologia, Centro de Investigação em Biomedicina, Universidade do Algarve Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Faleiro
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001439:Bacteroides / Q000378:metabolism*; D002648:Child; D003013:Clostridium / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005243:Feces / Q000737:chemistry; D005247:Feeding Behavior; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008297:Male; D020543:Proteome / Q000032:analysis*; D011795:Surveys and Questionnaires
chemical_list: D001426:Bacterial Proteins; D020543:Proteome