title: In vitro cytotoxicity assessment of roundup (glyphosate) in L-02 hepatocytes.
abstract: The goal of the present study was to elucidate the in vitro cytotoxicity of Roundup and to reveal the possible related mechanisms in L-02 hepatocytes. By detecting reactive oxygen species (ROS) production, glutathione (GSH)/superoxide dismutase (SOD) levels, mitochondrial permeability transition pore (PTP) open rate, apoptosis-inducing factor (AIF) release, intracellular Ca2+concentration, and alanine aminotransferease (ALT)/aspartate aminotransferase (AST) leakage, we determined that Roundup induced anti-oxidant system inhibition, mitochondria damage, DNA damage, membrane integrity and permeability changes, and apoptosis in L-02 hepatocytes. By revealing the mechanistic insights of Roundup-induced cytotoxicity, our results are valuable for the design of preventive and therapeutic strategies for the occupational population exposed to Roundup and other pesticides.
authors.affiliation: a Changsha Center for Disease Control and Prevention , Changsha , PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.affiliation: b Hunan Provincial Center for Disease Control and Prevention , Changsha , PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: c Department of Health Toxicology , Xiangya School of Public Health, Central South University , Changsha , PR China.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: c Department of Health Toxicology , Xiangya School of Public Health, Central South University , Changsha , PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zeng
authors.affiliation: c Department of Health Toxicology , Xiangya School of Public Health, Central South University , Changsha , PR China.
authors.forename: Caigao
authors.initials: C
authors.lastname: Zhong
authors.affiliation: c Department of Health Toxicology , Xiangya School of Public Health, Central South University , Changsha , PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xiao
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D022781:Hepatocytes / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*; D000097797:Glyphosate
chemical_list: D000410:Alanine Transaminase; D001219:Aspartate Aminotransferases; D002118:Calcium; D005978:Glutathione; D005979:Glutathione Peroxidase; D005998:Glycine; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000097797:Glyphosate
keywords: Cytotoxicity; L-02 hepatocytes; ROS; Roundup; glyphosate