title: Comparative Analysis of Salivary Alkaline Phosphatase in Post menopausal Women with and without Periodontitis.
abstract: BACKGROUND
  Alkaline phosphatase is an intracellular destruction enzyme in the periodontium, and it takes part in the normal turnover of the periodontal ligament, alveolar bone, and root cementum formation and maintenance.
  
  
  OBJECTIVE
  The aim of this case control study was to evaluate the enzyme Alkaline Phosphatase (ALP) level in saliva of post menopausal women with and without chronic periodontitis.
  
  
  METHODS
  In this study, 40 individuals, satisfying the study inclusion and exclusion criteria, were recruited. They were categorically divided, on the basis of gingival index, probing pocket depth and clinical attachment level, into two groups: Group I (post menopausal women with a clinically healthy periodontium, n=20); and Group II (post menopausal women with generalized chronic periodontitis, n=20). Clinical parameters assessed were Plaque Index (PI), Gingival Index (GI), Clinical Attachment Level (CAL) and Probing Pocket Depth (PPD). Unstimulated salivary samples were obtained in which the ALP concentration was measured using p-Nitrophenylphosphate, and 2-amino-2-methyl-1-propanol reagents in Beckman and Coulter, AU 480 auto analyser. Mann-Whitney U test was used to find statistical difference with respect to all clinical parameters such as PI, GI, CAL, PPD and salivary ALP levels.
  
  
  RESULTS
  The mean ALP in saliva was found to be higher in Group II compared to Group I and the difference was statistically significant with the p-value of 0.008.
  
  
  CONCLUSIONS
  A noteworthy increase in the ALP concentration was seen in saliva in our study (Group II) may be due to increased periodontal inflammation in post menopausal women. Thus salivary ALP can be taken as an additional biomarker to early diagnosis, development and progression of periodontitis especially among post menopausal women.
authors.affiliation: Postgraduate Student, Department of Periodontics, Thaimoogambigai Dental College , Chennai, Tamil Nadu, India .
authors.forename: Khumukcham
authors.initials: K
authors.lastname: Sophia
authors.affiliation: Professor, Department of Periodontics, Thaimoogambigai Dental College , Chennai, Tamil Nadu, India .
authors.forename: Snophia
authors.initials: S
authors.lastname: Suresh
authors.affiliation: Professor and Head, Department of Periodontics, Thaimoogambigai Dental College , Chennai, Tamil Nadu, India .
authors.forename: Uma
authors.initials: U
authors.lastname: Sudhakar
authors.affiliation: Postgraduate Student, Department of Periodontics, Thaimoogambigai Dental College , Chennai, Tamil Nadu, India .
authors.forename: Parvathee
authors.initials: P
authors.lastname: Jayakumar
authors.affiliation: Senior Lecturer, Department of Periodontics, Thaimoogambigai Dental College , Chennai, Tamil Nadu, India .
authors.forename: Danny
authors.initials: D
authors.lastname: Mathew
keywords: Alveolar bone loss; Biomarker; Menopause; Oestrogen; Saliva