title: Characterization of isolates of Eisenbergiella tayi, a strictly anaerobic Gram-stain variable bacillus recovered from human clinical materials in Canada.
abstract: Eisenbergiella gen. nov. was proposed in 2014 to describe an obligate anaerobic, structurally Gram-positive but Gram-stain-negative-appearing bacillus recovered from the blood culture of an elderly Israeli man. Here, we describe features for eight blood culture isolates as well one appendix-derived isolate, recovered from seven patients located in two Canadian provinces, which by 16S rRNA gene sequencing, were identifiable as Eisenbergiella tayi, the sole validly- named species in this genus. After whole genome sequencing, isolates were found to be essentially identical (96.8-98.7% identity) to each other and to E. tayi DSM 26961T, after comparison using the ANIb tool and in silico DNA-DNA hydridization. All isolates were observed to have remarkably large genomes (7.1-8.3 Mb) with a G + C content of 46.5%-46.9%.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada Winnipeg, MB Canada; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada. Electronic address: Kathy.Bernard@phac-aspc.gc.ca.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Bernard
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada Winnipeg, MB Canada.
authors.forename: Tamara
authors.initials: T
authors.lastname: Burdz
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada Winnipeg, MB Canada.
authors.forename: Deborah
authors.initials: D
authors.lastname: Wiebe
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada Winnipeg, MB Canada.
authors.forename: Brittany M
authors.initials: BM
authors.lastname: Balcewich
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada Winnipeg, MB Canada.
authors.forename: Tina
authors.initials: T
authors.lastname: Zimmerman
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada; Department of Clinical Microbiology, St. Boniface General Hospital, Winnipeg, MB, Canada.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lagacé-Wiens
authors.affiliation: British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, BC, Canada.
authors.forename: Linda M N
authors.initials: LMN
authors.lastname: Hoang
authors.affiliation: Université de Saint-Boniface, Winnipeg, MB, Canada.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Bernier
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001064:Appendicitis / Q000382:microbiology; D016470:Bacteremia / Q000382:microbiology; D001431:Bacteriological Techniques; D001482:Base Composition; D002170:Canada; D000068016:Eubacteriales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D016680:Genome, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Clostridiales cluster XIVa; Eisenbergiella tayi from human blood cultures; Whole genome sequencing