title: In vitro cytotoxicity and antimicrobial activity of Talaromyces flavus SP5 inhabited in the marine sediment of Southern Coast of India.
abstract: Marine sediment samples were collected from the coastal areas of Southern India, particularly in Kanyakumari District. Twenty-eight different fungal strains were isolated. The screening of fungi from marine sediment was done to isolate a potent fungus that can produce bioactive compounds for biomedical applications. Only three strains viz Trichoderma gamsii SP4, Talaromyces flavus SP5 and Aspergillus oryzae SP6 were screened for further studies. The intracellular bioactive compounds were extracted using solvent extraction method. The crude extracts were tested for its anti-microbial and anti-cancer properties and analytically characterized using Gas Chromatography Mass Spectrometry (GC-MS). All the three extracts were active, but the extract from T. flavus SP5 was found to be more active against various human pathogens, viz., Pseudomonas aeruginosa ATCC 27853 (17.8 ± 0.1), Escherichia coli ATCC 52922 (18.3 ± 0.3), and Candida tropicalis ATCC 750 (17.7 ± 0.4). It also exhibited cytotoxic activity against HEp2 carcinoma cell line with the LC50value of 25.7 μg·L-1. The GC-MS data revealed the presence of effective bioactive compounds. These results revealed that the extract from isolated fungus T. flavus SP5 acted as a potent antimicrobial, antifungal, and anticancer agent, providing basic information on the potency of marine fungi towards biomedical applications; further investigation may lead to the development of novel anticancer drugs.
authors.affiliation: Department of Biotechnology, Udaya School of Engineering, Udaya Nagar, Tamil Nadu, India; Center for Biologically Inspired Systems Science, Department of Biology, Indian Institute of Technology Jodhpur, Old Residency Road, Jodhpur, Rajasthan, India.
authors.forename: Bibin G
authors.initials: BG
authors.lastname: Anand
authors.affiliation: Department of Biotechnology, Udaya School of Engineering, Udaya Nagar, Tamil Nadu, India.
authors.forename: C K Navin
authors.initials: CK
authors.lastname: Thomas
authors.affiliation: Department of Biotechnology, Udaya School of Engineering, Udaya Nagar, Tamil Nadu, India; Cherraan's Institute of Health Sciences, Tamil Nadu, India. Electronic address: pra_cas@yahoo.co.in.
authors.forename: S
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D005658:Fungi / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D032901:Talaromyces / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents
keywords: Anti-microbial; Anticancer agents; Bioactive compounds; Marine sediment fungi