title: Role of Autophagy in Angiogenesis Induced by a High-Glucose Condition in RF/6A Cells.
abstract: OBJECTIVE
  To study the effect of autophagy on vitality, migration, and tube formation of RF/6A cells under the condition of D-glucose.
  
  
  METHODS
  Cultured RF/6A cells were randomly divided into 4 groups (control, low glucose, high glucose, and high glucose with 3-methyladenine [3-MA]). Autophagy-related proteins (Atg7, p62, and LC3) were monitored. Cell vitality, cell migration, tube formation, reactive oxygen species (ROS) production, and apoptosis were assessed.
  
  
  RESULTS
  Cell vitality significantly decreased and cell migration and tube formation significantly increased in the high-glucose group (p < 0.05). Pretreatment with 3-MA significantly increased cell viability and inhibited cell migration and tube formation (p < 0.05). ROS production increased in the high-glucose group and decreased in the high-glucose with N-acetylcysteine (NAC) group (p < 0.05). The level of apoptosis increased in the high-glucose group, while it was reduced in the high-glucose with 3-MA group.
  
  
  CONCLUSIONS
  Autophagy maybe participates in the formation of retinal neovascularization induced by high glucose.
authors.affiliation: Department of Ophthalmology, Xi'an Ninth Hospital Affiliated to Medical College of Xi'an Jiaotong University, Xi'an, China.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Du
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Bai-Xiang
authors.initials: BX
authors.lastname: Cheng
authors.forename: Manzila
authors.initials: M
authors.lastname: Kuerbanjiang
authors.forename: Le
authors.initials: L
authors.lastname: Ma
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D015153:Blotting, Western; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D003930:Diabetic Retinopathy / Q000150:complications* / Q000473:pathology; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D017382:Reactive Oxygen Species / Q000378:metabolism; D015861:Retinal Neovascularization / Q000209:etiology / Q000378:metabolism / Q000473:pathology*
chemical_list: D017382:Reactive Oxygen Species; D005947:Glucose