title: Identification of an acidic endo-polygalacturonase from Penicillium oxalicum CZ1028 and its broad use in major tropical and subtropical fruit juices production.
abstract: Endo-polygalacturonases play an important role on depectinization in fruit juices industry. A putative endo-polygalacturonase gene PoxaEnPG28A was cloned from Penicillium oxalicum CZ1028. PoxaEnPG28A consisted of a putative signal peptide and a catalytic domain belonging to glycoside hydrolase family 28, and it shared 72% identity with that of a functionally characterized endo-polygalacturonase from Trichoderma harzianum. Gene PoxaEnPG28A was successfully expressed in Pichia pastoris with a high yield of 1828.7 U/mL. The purified recombinant enzyme PoxaEnPG28A hydrolyzed polygalacturonic acid in endo-manner releasing oligo-galacturonates. PoxaEnPG28A showed maximal activity at pH 5.5 and 55°C, and was stable between pH 3.0 to 10.0 and below 45°C. The kinetic constants Kmand Vmaxof PoxaEnPG28A were calculated as 1.57 g/L and 14,641.29 U/mg, respectively. PoxaEnPG28A significantly improved the yields of fruit juices from banana, plantain, papaya, pitaya and mango. The high production level of the recombinant enzyme PoxaEnPG28A by P. pastoris and remarkable catalytic activity of PoxaEnPG28A toward five kinds of fruit juices made the enzyme a potential application in agriculture and food industries.
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xian
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
authors.forename: Yutuo
authors.initials: Y
authors.lastname: Wei
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Hongchi
authors.initials: H
authors.lastname: Tang
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Lu
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Qixia
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Guangxi Teachers Education University, Mingxiudong Road, Nanning 530001, China.
authors.forename: Yunlai
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China. Electronic address: rbhuang@gxas.ac.cn.
authors.forename: Ribo
authors.initials: R
authors.lastname: Huang
mesh_terms: D001709:Biotechnology; D003001:Cloning, Molecular; D004795:Enzyme Stability; D000067030:Fruit and Vegetable Juices; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010368:Pectins / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D010843:Pichia / Q000235:genetics; D011096:Polygalacturonase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D014329:Tropical Climate
chemical_list: D010368:Pectins; D011096:Polygalacturonase; C003181:polygalacturonic acid
keywords: Endo-polygalacturonase; Enzymatic characterization; Fruit juice; Gene expression; Pectin; Penicillium oxalicum