title: Production of verbascoside and phenolic acids in biomass of Verbena officinalis L. (vervain) cultured under different in vitro conditions.
abstract: Methanolic extracts from the biomass of Verbena officinalis cultured under continuous artificial light and in darkness on 12 variants of the Murashige and Skoog medium containing different concentrations (0.5-3.0 mg/L) of plant growth regulators: 6-benzyladenine, kinetin, 1-naphthaleneacetic acid and indole-3-butyric acid, were analysed for the amounts of verbascoside and phenolic acids, before and after acid hydrolysis, using the HPLC-DAD method. The amounts of verbascoside were very high (max. 2454.12 mg/100 g DW - light, and 2135.59 mg/100 g DW - darkness). The total amounts of phenolic acids reached a maximum of 46.02 mg/100 g DW (free phenolic acids) and 141.05 mg/100 g DW (bound compounds). The main metabolites were: ferulic, o-coumaric and caffeic acids. The maximum amount of verbascoside was 3.28 times higher than in extracts from the herb of the parent plant. The cultures could be proposed as a potential biotechnological source for selected biologically active compounds.
authors.affiliation: a Department of Pharmaceutical Botany, Medical College , Jagiellonian University , Kraków , Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Kubica
authors.affiliation: a Department of Pharmaceutical Botany, Medical College , Jagiellonian University , Kraków , Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szopa
authors.affiliation: a Department of Pharmaceutical Botany, Medical College , Jagiellonian University , Kraków , Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Ekiert
mesh_terms: D001593:Benzyl Compounds; D018533:Biomass; D002109:Caffeic Acids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides; D062385:Hydroxybenzoates / Q000138:chemical synthesis*; D007211:Indoles; D007701:Kinetin; D009280:Naphthaleneacetic Acids; D010636:Phenols; D010936:Plant Extracts / Q000737:chemistry*; D010937:Plant Growth Regulators / Q000032:analysis; D011687:Purines; D032412:Verbena / Q000737:chemistry* / Q000254:growth & development; D059808:Polyphenols
chemical_list: D001593:Benzyl Compounds; D002109:Caffeic Acids; D005960:Glucosides; D062385:Hydroxybenzoates; D007211:Indoles; D007701:Kinetin; D009280:Naphthaleneacetic Acids; D010636:Phenols; D010936:Plant Extracts; D010937:Plant Growth Regulators; D011687:Purines; C014612:indolebutyric acid; C034182:1-naphthaleneacetic acid; C058956:acteoside; D059808:Polyphenols; C017616:phenolic acid; C480551:benzylaminopurine
keywords: HPLC-DAD analysis; White light; bound phenolic acids; darkness; free phenolic acids; plant growth regulators