title: Genetic and Transgenic Reagents forDrosophila simulans,D. mauritiana,D. yakuba,D. santomea, andD. virilis.
abstract: Species of theDrosophila melanogasterspecies subgroup, including the speciesD. simulans,D. mauritiana,D. yakuba, andD. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mappedpiggyBactransposons including anenhanced yellow fluorescent protein(EYFP) gene expressed in the eyes and a ϕC31 attPsite-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements inD. virilisthat will be useful for genetic mapping studies.
authors.affiliation: Janelia Research Campus, Ashburn, Virginia 20147 sternd@janelia.hhmi.org.
authors.forename: David L
authors.identifier: 0000-0002-1847-6483
authors.initials: DL
authors.lastname: Stern
authors.affiliation: Janelia Research Campus, Ashburn, Virginia 20147.
authors.forename: Justin
authors.initials: J
authors.lastname: Crocker
authors.affiliation: Janelia Research Campus, Ashburn, Virginia 20147.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Departamento de Ecologıa, Genetica y Evolucion, Instituto de Ecología, Genética y Evolución de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA, Argentina.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Frankel
authors.affiliation: Department of Ecology and Evolutionary Biology, and.
authors.forename: Gretchen
authors.initials: G
authors.lastname: Kappes
authors.affiliation: Janelia Research Campus, Ashburn, Virginia 20147.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Kim
authors.affiliation: Genetics Division, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115.
authors.forename: Ryan
authors.initials: R
authors.lastname: Kuzmickas
authors.affiliation: Janelia Research Campus, Ashburn, Virginia 20147.
authors.forename: Andrew
authors.initials: A
authors.lastname: Lemire
authors.affiliation: Department of Biological Sciences, California State University East Bay, Hayward, California 94542.
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Mast
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Carl Icahn Laboratory, Princeton University, New Jersey 08544.
authors.forename: Serge
authors.initials: S
authors.lastname: Picard
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D000067636:Drosophila simulans / Q000235:genetics; D005123:Eye / Q000378:metabolism; D005786:Gene Expression Regulation; D023281:Genomics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000235:genetics; D013045:Species Specificity; D019076:Transgenes
chemical_list: D004251:DNA Transposable Elements; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Drosophila; evolution; genetics; speciation; transgenics; ϕC31 integrase