title: Dual recombinant Lactococcus lactis for enhanced delivery of DNA vaccine reporter plasmid pPERDBY.
abstract: Food grade Lactococcus lactis has been widely used as an antigen and DNA delivery vehicle. We have previously reported the use of non-invasive L. lactis to deliver the newly constructed immunostimulatory DNA vaccine reporter plasmid, pPERDBY. In the present report, construction of dual recombinant L. lactis expressing internalin A of Listeria monocytogenes and harboring pPERDBY (LL InlA + pPERDBY) to enhance the efficiency of delivery of DNA by L. lactis is outlined. After confirmation and validation of LL InlA + pPERDBY, its DNA delivery potential was compared with previously developed non-invasive r- L. lactis::pPERDBY. The use of invasive L. lactis resulted in around threefold increases in the number of enhanced green fluorescent protein-expressing Caco-2 cells. These findings reinforce the prospective application of invasive strain of L. lactis for delivery of DNA/RNA and antigens.
authors.affiliation: Department of Cell and Molecular Biology, B. V. Patel Pharmaceutical Education and Research Development Centre, Ahmedabad, Gujarat.|B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidhyanagar, Gujarat.
authors.forename: Bhrugu
authors.initials: B
authors.lastname: Yagnik
authors.affiliation: Department of Cell and Molecular Biology, B. V. Patel Pharmaceutical Education and Research Development Centre, Ahmedabad, Gujarat.|B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidhyanagar, Gujarat.
authors.forename: Drashya
authors.initials: D
authors.lastname: Sharma
authors.affiliation: Sardar Patel University, Vallabh Vidhyanagar, Gujarat, India.
authors.forename: Harish
authors.initials: H
authors.lastname: Padh
authors.affiliation: Department of Cell and Molecular Biology, B. V. Patel Pharmaceutical Education and Research Development Centre, Ahmedabad, Gujarat.
authors.forename: Priti
authors.initials: P
authors.lastname: Desai
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018938:Caco-2 Cells; D004337:Drug Carriers; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D013294:Lactococcus lactis / Q000235:genetics*; D010957:Plasmids; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004337:Drug Carriers; D019444:Vaccines, DNA; C069761:internalin protein, Bacteria; D049452:Green Fluorescent Proteins
keywords: DNA delivery; Lactococcus lactis; enhanced green fluorescent protein; invasive