title: Concentration-dependent linkage of dietary methionine restriction to the components of its metabolic phenotype.
abstract: Restricting dietary methionine to 0.17% produces a series of physiological responses through coordinated transcriptional effects in liver and adipose tissue. The goal of the present work was to determine the threshold concentrations above and below 0.17% at which the beneficial responses to 0.17% dietary methionine are preserved.
  
  
  
  Diets were formulated to restrict methionine to different degrees, followed by evaluation of the transcriptional and physiological responses to the different diets.
  
  
  
  Restriction of dietary methionine to 0.25%, but not 0.34%, was partially effective in reproducing the metabolic phenotype produced by restriction of methionine to 0.17%, while restriction of methionine to 0.12% reproduced the responses produced by restriction to 0.17% but failed to support growth and caused excessive weight loss. Restriction beyond 0.12% initiated responses characteristic of essential amino acid deprivation including food aversion and rapid weight loss.
  
  
  
  Restriction of dietary methionine to levels above 0.25% was without effect, while restriction to levels below 0.12% produced responses characteristic of essential amino acid deprivation. In addition, although restriction of dietary methionine to 0.12% did not evoke essential amino acid deprivation responses, it provided insufficient methionine to support growth. The ideal range of dietary methionine restriction was from 0.17% to 0.25%.
authors.affiliation: Laboratory of Nutrient Sensing & Adipocyte Signaling, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Forney
authors.affiliation: Department of Nutrition, Georgia State University, Atlanta, Georgia, USA.
authors.forename: Desiree
authors.initials: D
authors.lastname: Wanders
authors.affiliation: Laboratory of Nutrient Sensing & Adipocyte Signaling, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
authors.forename: Kirsten P
authors.initials: KP
authors.lastname: Stone
authors.affiliation: Laboratory of Nutrient Sensing & Adipocyte Signaling, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Pierse
authors.affiliation: Laboratory of Nutrient Sensing & Adipocyte Signaling, Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
authors.forename: Thomas W
authors.identifier: 0000-0001-7125-7995
authors.initials: TW
authors.lastname: Gettys
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D004032:Diet / Q000379:methods*; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000008:administration & dosage* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D064167:Recommended Dietary Allowances; D012016:Reference Values
chemical_list: D004044:Dietary Proteins; D008715:Methionine