title: Controlled PEGylation Crowdedness for Polymeric Micelles To Pursue Ligand-Specified Privileges as Nucleic Acid Delivery Vehicles.
abstract: A facile poly(ethylene glycol) (PEG) detachment scheme was utilized to control the PEGylation degree of the polymeric micelles. The performance of cyclic Arg-Gly-Asp (cRGD) as a targeted moiety was studied on a class of polymeric micelles with various PEGylation degrees, revealing that the specific cRGD-mediated cell affinity, thus the cellular uptake and implicated privileges including the ligand-specified favorable intracellular trafficking and consequent favorable biofunctions, was prominent for the polymeric micelles with high PEGylation degree. These results endow important information and implications for the design and development of targeted nanomedicine, particularly the delivery of vulnerable biological compounds.
authors.affiliation: School of Public Health, Dalian Medical University , No. 9 West Section, Lvshun South Road, Dalian 116044, China.
authors.forename: Xiyi
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Science, Dalian Ocean University , No. 52 Heishijiao Street, Dalian 116023, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Gu
authors.affiliation: School of Environmental Science and Safety Engineering, Tianjin University of Technology , Xiqing District, Tianjing 300384, China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Ningbo Institute of Materials Technology and Engineering, China Academy of Sciences , Ningbo 315201, China.
authors.forename: Sudong
authors.initials: S
authors.lastname: Wu
authors.affiliation: Ningbo Hygeia Medical Technology Company, Ltd. , No. 1177 Lingyun Road, High-Tech Zone, Ningbo 315040, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai 200127, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Ningbo Hygeia Medical Technology Company, Ltd. , No. 1177 Lingyun Road, High-Tech Zone, Ningbo 315040, China.
authors.forename: Qixian
authors.identifier: http://orcid.org/0000-0002-3091-671X
authors.initials: Q
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D008024:Ligands; D008823:Micelles; D009696:Nucleic Acids; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D008024:Ligands; D008823:Micelles; D009696:Nucleic Acids; D011092:Polyethylene Glycols
keywords: PEGylation; cyclic RGD; endosome escape; mRNA delivery; polymeric micelle