title: Isolation of a peptide from Ph.D.-C7C phage display library for detection of Cry1Ab.
abstract: Traditional ELISA methods of using animal immunity yield antibodies for detection Cry toxin. Not only is this incredibly harmful to the animals, but is also time-intensive. Here we developed a simple method to yield the recognition element. Using a critical selection strategy and immunoassay we confirmed a clone from the Ph.D-C7C phage library, which has displayed the most interesting Cry1Ab-binding characteristics examined in this study (Fig. 1). The current study indicates that isolating peptide is an alternative method for the preparation of a recognition element, and that the developed assay is a potentially useful tool for detecting Cry1Ab.
authors.affiliation: College of Horticulture, Jinling Institute of Technology, 210038 Nanjing, PR China. Electronic address: yuebaiheapple@sina.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Horticulture, Jinling Institute of Technology, 210038 Nanjing, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Key Laboratory of Control Technology and Standard for Agro- Product Safety and Quality, Ministry of Agriculture, Institute of Food Quality Safety and Detection Research, Jiangsu Academy of Agricultural Sciences, 210014 Nanjing, PR China.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Wu
authors.affiliation: College of Horticulture, Jinling Institute of Technology, 210038 Nanjing, PR China.
authors.forename: Zhen-Ping
authors.initials: ZP
authors.lastname: Hao
authors.affiliation: Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Key Laboratory of Control Technology and Standard for Agro- Product Safety and Quality, Ministry of Agriculture, Institute of Food Quality Safety and Detection Research, Jiangsu Academy of Agricultural Sciences, 210014 Nanjing, PR China.
authors.forename: Xian-Jin
authors.initials: XJ
authors.lastname: Liu
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001435:Bacteriophages / Q000378:metabolism*; D004731:Endotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006460:Hemolysin Proteins / Q000032:analysis* / Q000276:immunology; D057230:Limit of Detection; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019151:Peptide Library; D010455:Peptides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: BBMV-Peptide sandwich-ELISA; Cry1Ab; Phage display