title: Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface.
abstract: Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.
authors.affiliation: Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Würzburg, Germany. muhammad@biozentrum.uni-wuerzburg.de.|Department of Molecular Biology and Genetics, Boğaziçi University, North Campus Kuzey Park Building 310, 34342, Bebek, Instanbul, Turkey. muhammad@biozentrum.uni-wuerzburg.de.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Naseem
authors.affiliation: Department of Animal Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
authors.forename: Shabana
authors.initials: S
authors.lastname: Shams
authors.affiliation: Department of Plant and Environmental Sciences, Copenhagen Plant Science Centre, University of Copenhagen, Taastrup, Denmark.|Global Change Research Centre, Czech Globe AS CR, v.v.i., Drásov 470, 664 24, Drásov, Czech Republic.
authors.forename: Thomas
authors.initials: T
authors.lastname: Roitsch
mesh_terms: D003583:Cytokinins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions / Q000276:immunology; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D057865:Plant Immunity; D030821:Plants, Genetically Modified; D015398:Signal Transduction; D014170:Transformation, Genetic
chemical_list: D003583:Cytokinins; D010937:Plant Growth Regulators
keywords: Cytokinins; Host-pathogen interaction; Pathogen-inducible promoter; Transient plant transformation