title: Multi-layered inhibition of Streptomyces development: BldO is a dedicated repressor of whiB.
abstract: BldD-(c-di-GMP) sits on top of the regulatory network that controls differentiation in Streptomyces, repressing a large regulon of developmental genes when the bacteria are growing vegetatively. In this way, BldD functions as an inhibitor that blocks the initiation of sporulation. Here, we report the identification and characterisation of BldO, an additional developmental repressor that acts to sustain vegetative growth and prevent entry into sporulation. However, unlike the pleiotropic regulator BldD, we show that BldO functions as the dedicated repressor of a single key target gene, whiB, and that deletion of bldO or constitutive expression of whiB is sufficient to induce precocious hypersporulation.
authors.affiliation: Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Matthew J
authors.identifier: http://orcid.org/0000-0001-8216-0152
authors.initials: MJ
authors.lastname: Bush
authors.affiliation: Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Govind
authors.initials: G
authors.lastname: Chandra
authors.affiliation: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Kim C
authors.initials: KC
authors.lastname: Findlay
authors.affiliation: Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Buttner
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D005798:Genes, Bacterial; D018085:Regulon; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C111306:BldD protein, Streptomyces coelicolor; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors