title: Biochemical and genetic toxicity of dinotefuran on earthworms (Eisenia fetida).
abstract: Dinotefuran is a third-generation neonicotinoid insecticide, that is considered promising due to its excellent properties. In the present work, the biochemical and genetic toxicity of dinotefuran on earthworms were evaluated at a series of environmental background concentrations. Meanwhile, the effective concentrations of dinotefuran in artificial soil during the entire exposure period were monitored. The present results showed that dinotefuran was stable in artificial soil, and its concentrations changed no more than 20% during the 28-d exposure. At 1.0 mg/kg and 2.0 mg/kg, dinotefuran induced excess generation of ROS, resulting in significant changes in antioxidant enzyme activities and functional gene expression. Moreover, lipids, proteins and nucleic acids were oxidized and damaged by the excess ROS induced by dinotefuran, resulting in serious destruction of the structure and function of cells. Additionally, the toxicity of dinotefuran showed obvious dose- and time-dependent effects. Therefore, we consider that dinotefuran may be a high-risk pollutant for earthworms.
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao, 266101, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao, 266101, PR China. Electronic address: wangxiuguo@caas.cn.
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao, 266101, PR China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Xu
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao, 266101, PR China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: You
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao, 266101, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao, 266101, PR China.
authors.forename: Fenglong
authors.initials: F
authors.lastname: Wang
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao, 266101, PR China. Electronic address: liyiqiang@caas.cn.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D004781:Environmental Exposure / Q000009:adverse effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006146:Guanidines / Q000378:metabolism / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D009835:Oligochaeta / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
chemical_list: D006146:Guanidines; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D017382:Reactive Oxygen Species; D012987:Soil; D012989:Soil Pollutants; C465368:dinotefuran
keywords: GR; HSP70; MT; Neonicotinoid insecticides; Phagocytosis; ROS