title: A synthetic redox biofilm made from metalloprotein-prion domain chimera nanowires.
abstract: Engineering bioelectronic components and set-ups that mimic natural systems is extremely challenging. Here we report the design of a protein-only redox film inspired by the architecture of bacterial electroactive biofilms. The nanowire scaffold is formed using a chimeric protein that results from the attachment of a prion domain to a rubredoxin (Rd) that acts as an electron carrier. The prion domain self-assembles into stable fibres and provides a suitable arrangement of redox metal centres in Rd to permit electron transport. This results in highly organized films, able to transport electrons over several micrometres through a network of bionanowires. We demonstrate that our bionanowires can be used as electron-transfer mediators to build a bioelectrode for the electrocatalytic oxygen reduction by laccase. This approach opens opportunities for the engineering of protein-only electron mediators (with tunable redox potentials and optimized interactions with enzymes) and applications in the field of protein-only bioelectrodes.
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Altamura
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Horvath
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.|Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Rengaraj
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.|Université Grenoble Alpes, INAC-SPrAM, F-38000 Grenoble, France.|CNRS, INAC-SPrAM, F-38000 Grenoble, France.|CEA, INAC-SPrAM, F-38000 Grenoble, France.
authors.forename: Anaëlle
authors.initials: A
authors.lastname: Rongier
authors.affiliation: Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Kamal
authors.initials: K
authors.lastname: Elouarzaki
authors.affiliation: Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Gondran
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.
authors.forename: Anthony L B
authors.initials: AL
authors.lastname: Maçon
authors.affiliation: ERRMECe, I-MAT FD4122, Université de Cergy-Pontoise, 2 avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.|LMGP, CNRS UMR 5628, 3 parvis Louis Néel, 38016 Grenoble, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Vendrely
authors.affiliation: Institut Néel, CNRS-UJF-INP, 38042 Grenoble Cedex 09, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Bouchiat
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.|Laboratoire de Chimie des Processus Biologiques, UMR 8229 CNRS, Université Pierre et Marie Curie - Paris 6, Collège de France. 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Fontecave
authors.affiliation: CEA, LETI, MINATEC Campus, F-38054 Grenoble, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Mariolle
authors.affiliation: Université Grenoble Alpes, INAC-SPrAM, F-38000 Grenoble, France.|CNRS, INAC-SPrAM, F-38000 Grenoble, France.|CEA, INAC-SPrAM, F-38000 Grenoble, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Rannou
authors.affiliation: Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Alan
authors.initials: A
authors.lastname: Le Goff
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Duraffourg
authors.affiliation: Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.|CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Holzinger
authors.affiliation: Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.|CNRS, BIG-LCBM, F-38000 Grenoble, France.|CEA, BIG-LCBM, F-38000 Grenoble, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Forge
mesh_terms: D002384:Catalysis; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D042845:Laccase / Q000737:chemistry / Q000378:metabolism; D008667:Metalloproteins / Q000737:chemistry*; D017017:Methanococcus / Q000378:metabolism; D018625:Microscopy, Atomic Force; D053770:Nanowires / Q000737:chemistry*; D010084:Oxidation-Reduction; D011328:Prions / Q000737:chemistry*; D012416:Rubredoxins / Q000737:chemistry*
chemical_list: D008667:Metalloproteins; D011328:Prions; D012416:Rubredoxins; D042845:Laccase