title: Tex19paralogs are new members of the piRNA pathway controlling retrotransposon suppression.
abstract: Tex19genes are mammalian specific and duplicated to giveTex19.1andTex19.2in some species, such as the mouse and rat. It has been demonstrated that mutantTex19.1males display a variable degree of infertility whereas they all upregulate MMERVK10C transposons in their germ line. In order to study the function of both paralogs in the mouse, we generated and studiedTex19double knockout (Tex19DKO) mutant mice. AdultTex19DKO males exhibited a fully penetrant phenotype, similar to the most severe phenotype observed in the singleTex19.1KO mice, with small testes and impaired spermatogenesis, defects in meiotic chromosome synapsis, persistence of DNA double-strand breaks during meiosis, lack of post-meiotic germ cells and upregulation of MMERVK10C expression. The phenotypic similarities to mice with knockouts in thePiwifamily genes prompted us to check and then demonstrate, by immunoprecipitation and GST pulldown followed by mass spectrometry analyses, that TEX19 paralogs interact with PIWI proteins and the TEX19 VPTEL domain directly binds Piwi-interacting RNAs (piRNAs) in adult testes. We therefore identified two new members of the postnatal piRNA pathway.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de Santé et de Recherche Médicale (INSERM) U964/Centre National de Recherche Scientifique (CNRS) UMR 1704/Université de Strasbourg, Illkirch 67404, France.
authors.forename: Yara
authors.identifier: 0000-0001-5692-8674
authors.initials: Y
authors.lastname: Tarabay
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de Santé et de Recherche Médicale (INSERM) U964/Centre National de Recherche Scientifique (CNRS) UMR 1704/Université de Strasbourg, Illkirch 67404, France.
authors.forename: Mayada
authors.identifier: 0000-0003-1044-824X
authors.initials: M
authors.lastname: Achour
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de Santé et de Recherche Médicale (INSERM) U964/Centre National de Recherche Scientifique (CNRS) UMR 1704/Université de Strasbourg, Illkirch 67404, France.|Service de Biologie de la Reproduction, Centre Hospitalier Universitaire, Strasbourg 67000, France.
authors.forename: Marius
authors.initials: M
authors.lastname: Teletin
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de Santé et de Recherche Médicale (INSERM) U964/Centre National de Recherche Scientifique (CNRS) UMR 1704/Université de Strasbourg, Illkirch 67404, France.
authors.forename: Tao
authors.initials: T
authors.lastname: Ye
authors.affiliation: Institut Curie, department of Genetics and Developmental Biology, CNRS UMR3215, INSERM U934, 75005 Paris, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Teissandier
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de Santé et de Recherche Médicale (INSERM) U964/Centre National de Recherche Scientifique (CNRS) UMR 1704/Université de Strasbourg, Illkirch 67404, France.|Service de Biologie de la Reproduction, Centre Hospitalier Universitaire, Strasbourg 67000, France.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mark
authors.affiliation: Institut Curie, department of Genetics and Developmental Biology, CNRS UMR3215, INSERM U934, 75005 Paris, France.
authors.forename: Déborah
authors.initials: D
authors.lastname: Bourc'his
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de Santé et de Recherche Médicale (INSERM) U964/Centre National de Recherche Scientifique (CNRS) UMR 1704/Université de Strasbourg, Illkirch 67404, France stephane.viville@unistra.fr.|Centre Hospitalier Universitaire, Strasbourg 67000, France.
authors.forename: Stéphane
authors.identifier: 0000-0002-5088-3003
authors.initials: S
authors.lastname: Viville
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000235:genetics*; D005260:Female; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000235:genetics*; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins; D051381:Rats; D018626:Retroelements / Q000235:genetics*; D013489:Suppression, Genetic; D013737:Testis / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; D009687:Nuclear Proteins; C421088:PIWIL1 protein, human; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D018626:Retroelements; C528631:Tex19 protein, mouse
keywords: Infertility; Meiosis; Transposable elements; piRNA pathway