title: Fine-Mapping and Analysis ofCgl1, a Gene Conferring Glossy Trait in Cabbage (Brassica oleraceaL. var.capitata).
abstract: Cuticular waxes covering the outer plant surface impart a whitish appearance. Wax-less cabbage mutant shows glossy in leaf surface and plays important roles in riching cabbage germplasm resources and breeding brilliant green cabbage. This is the first report describing the characterization and fine-mapping of a wax biosynthesis gene using a novel glossyBrassica oleraceamutant. In the present paper, we identified a glossy cabbage mutant (line10Q-961) with a brilliant green phenotype. Genetic analyses indicated that the glossy trait was controlled by a single recessive gene. Preliminary mapping results using an F2population containing 189 recessive individuals revealed that theCgl1gene was located at the end of chromosome C08. Several new markers closely linked to the target gene were designed according to the cabbage reference genome sequence. Another population of 1,172 recessive F2individuals was used to fine-map theCgl1gene to a 188.7-kb interval between the C08SSR61 simple sequence repeat marker and the end of chromosome C08. There were 33 genes located in this region. According to gene annotation and homology analyses, theBol018504gene, which is a homolog ofCER1inArabidopsis thaliana, was the most likely candidate for theCgl1gene. Its coding and promoter regions were sequenced, which indicated that the RNA splice site was altered because of a 2,722-bp insertion in the first intron ofBol018504in the glossy mutant. Based on the FGENESH 2.6 prediction and sequence alignments, the PLN02869 domain, which controls fatty aldehyde decarbonylase activity, was absent from theBol018504gene of the 10Q-961 glossy mutant. We inferred that the inserted sequence inBol018504may result in the glossy cabbage mutant. This study represents the first step toward the characterization of cuticular wax biosynthesis inB. oleracea, and may contribute to the breeding of new cabbage varieties exhibiting a brilliant green phenotype.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural SciencesBeijing, China; College of Horticulture, China Agricultural UniversityBeijing, China.
authors.forename: Zezhou
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Mu
authors.initials: M
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yangyong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Honghao
authors.initials: H
authors.lastname: Lv
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Peitian
authors.initials: P
authors.lastname: Sun
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Horticulture, China Agricultural University Beijing, China.
authors.forename: Zhenxian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers - Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Yang
keywords: Cgl1; breeding; cabbage; fine-map; glossy mutant