title: GuideScan software for improved single and paired CRISPR guide RNA design.
abstract: We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.
authors.affiliation: Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.|Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.|Weill Cornell Graduate School of Medical Sciences of Cornell University, New York, New York, USA.
authors.forename: Alexendar R
authors.initials: AR
authors.lastname: Perez
authors.affiliation: Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Pritykin
authors.affiliation: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Joana A
authors.identifier: 0000-0003-2765-6985
authors.initials: JA
authors.lastname: Vidigal
authors.affiliation: Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.|Weill Cornell Graduate School of Medical Sciences of Cornell University, New York, New York, USA.
authors.forename: Sagar
authors.initials: S
authors.lastname: Chhangawala
authors.affiliation: Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Lee
authors.initials: L
authors.lastname: Zamparo
authors.affiliation: Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Christina S
authors.initials: CS
authors.lastname: Leslie
authors.affiliation: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ventura
mesh_terms: D000465:Algorithms; D064113:CRISPR-Cas Systems / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D020868:Gene Silencing; D000069550:Machine Learning; D034741:RNA, Small Interfering / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D012984:Software
chemical_list: D034741:RNA, Small Interfering