title: Nicotine can modulate the effects of the mesenchymal stem cells on neutrophils.
abstract: OBJECTIVE
  It has been revealed that mesenchymal stem cells (MSCs) express some of the nicotinic receptor subunits. Moreover, the crosstalk between MSCs and neutrophils is not far-fetched. Therefore, the aim of the present study is to determine the role of nicotine on the effects of MSCs on neutrophils.
  
  
  METHODS
  After the isolation of mesenchymal stem cells from the bone marrow of rats, these cells were pulsed with different concentrations of nicotine (0, 0.1, 0.5, and 1μM) for different periods (24, 48, and 72h). Then, the neutrophils were co-cultured with MSCs for 4h and the functions of neutrophils were evaluated.
  
  
  RESULTS
  The obtained findings showed that MSCs pulsed with nicotine significantly enhanced the viability and the phagocytic activity of co-cultured neutrophils and simultaneously, decreased the production of reactive oxygen substances (ROS), induced by f-MLP in neutrophils, more profound than MSCs without treatment. Moreover, MSCs, pulsed with nicotine at low to moderate concentrations, preserved the respiratory burst, triggered by opsonized yeast in neutrophils. Nevertheless, a high concentration of nicotine can interfere with the latter aspect of the crosstalk between MSCs and neutrophils.
  
  
  CONCLUSIONS
  The obtained data can offer a new insight into the potential mechanisms, underlying the immunomodulatory effects of nicotine.
authors.affiliation: Division of Immunology, Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Pourtayeb
authors.affiliation: Division of Immunology, Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran. Electronic address: meysamabtahi@hotmail.com.
authors.forename: Seyyed Meysam
authors.initials: SM
authors.lastname: Abtahi Froushani
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects
chemical_list: D018722:Nicotinic Agonists; D017382:Reactive Oxygen Species; D009538:Nicotine
keywords: Mesenchymal stem cell; Neutrophil; Nicotine