title: Interplay Between Auxin and Cytokinin and Its Impact on Mitogen Activated Protein Kinase (MAPK).
abstract: Plant physiology, in particular, is governed by a repertoire of endogenous as well as environmental cues. Auxin and cytokinin constitute an indispensable phytohormonal system required for plant growth and development. Another pivotal aspect of plant physiological process that thoroughly affects various plant growth and developmental attributes is the signaling network, majorly comprising the canonical mitogen activated protein kinase (MAPK) cascade. Striking a fine balance between the phytohormonal and signaling components could be adopted as an intricate strategy by plants to counteract various stresses in question. Thus, a brief understanding of this multifaceted complex could be of use for delineating numerous plant physiological and developmental phenomena. Thus, the present section discusses the various MAPK related assays in context to auxin and cytokinin crosstalk. Briefly, this chapter outlines the discrete MAPK methods to better understand the fundamentals of MAPK signaling network in auxin and cytokinin treated rice seedlings. Further, various phenotypic, genomic as well as proteomic protocols are discussed for a better understanding of MAPK networks in the backdrop of auxin and cytokinin interplay.
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110 067, India.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Singh
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110 067, India. alok@nipgr.ac.in.
authors.forename: Alok Krishna
authors.initials: AK
authors.lastname: Sinha
mesh_terms: D019295:Computational Biology / Q000379:methods; D003583:Cytokinins / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D023281:Genomics / Q000379:methods; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010641:Phenotype; D063245:Plant Development / Q000187:drug effects; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D012984:Software
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D020928:Mitogen-Activated Protein Kinases
keywords: Auxin; Cytokinin; Immunoblotting; In-gel kinase assay; MAPK assays; Mitogen activated protein kinase (MAPK); Root structure architecture (RSA); qRT-PCR