title: Novel ultrasound-responsive chitosan/perfluorohexane nanodroplets for image-guided smart delivery of an anticancer agent: Curcumin.
abstract: Ultrasound-responsive nanodroplets are a class of new emerging smart drug delivery systems which provide image-guided nano-therapy of various diseases, especially cancers. Here, we developed multifunctional smart curcumin-loaded chitosan/perfluorohexane nanodroplets for contrast-ultrasound imaging and on-demand drug delivery. The nanodroplets were synthesized via nanoemulsion process. The optimal formulation with the size of 101.2nm and 77.8% curcumin entrapment was chosen for release study and cytotoxicity evaluation. Sonication at the frequency of 1MHz, 2W/cm2for 4min triggered the release of 63.5% of curcumin from optimal formulation (Cur-NDs-2). Ultrasound aided release study indicated that the concentration of perfluorohexane and the degree of acoustic droplet vaporization play important role in ultrasound-active drug release. B-mode ultrasound imaging confirmed strong ultrasound contrast of chitosan nanodroplets even at low concentrations via droplet to bubble transition. Finally, cytotoxicity of the ultrasound-responsive nanodroplets in the presence of ultrasound was evaluated in-vitro on 4T1 human breast cancer cells. Cell growth inhibitory effects of curcumin-loaded nanodroplets significantly increased by ultrasound exposure. According to the obtained results, these ultrasound responsive curcumin-loaded chitosan/perfluorohexane nanodroplets have a great potential for imaged-guided cancer therapy.
authors.affiliation: Biomaterials Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, Iran. Electronic address: f.baghbani@aut.ac.ir.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Baghbani
authors.affiliation: Department of Chemistry, University of Ayatollah Alozma Boroujerdi, Boroujerd, Iran.
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Chegeni
authors.affiliation: Biomaterials Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, Iran.
authors.forename: Fathollah
authors.initials: F
authors.lastname: Moztarzadeh
authors.affiliation: Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 131451384, Tehran, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Hadian-Ghazvini
authors.affiliation: Biomaterials Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Raz
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003287:Contrast Media / Q000737:chemistry; D003474:Curcumin / Q000737:chemistry* / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005466:Fluorocarbons / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013010:Sonication; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D004337:Drug Carriers; D005466:Fluorocarbons; D048271:Chitosan; C078626:perflexane; D003474:Curcumin
keywords: Breast cancer; Chitosan/perfluorohexane nanodroplets; Contrast agent; Curcumin; Smart delivery; Ultrasound-responsive