title: Association Mapping Reveals Genetic Loci Associated with Important Agronomic Traits inLentinula edodes, Shiitake Mushroom.
abstract: Association mapping is a robust approach for the detection of quantitative trait loci (QTLs). Here, by genotyping 297 genome-wide molecular markers of 89Lentinula edodescultivars in China, the genetic diversity, population structure and genetic loci associated with 11 agronomic traits were examined. A total of 873 alleles were detected in the tested strains with a mean of 2.939 alleles per locus, and the Shannon's information index was 0.734. Population structure analysis revealed two robustly differentiated groups among the ChineseL. edodescultivars (FST= 0.247). Using the mixed linear model, a total of 43 markers were detected to be significantly associated with four traits. The number of markers associated with traits ranged from 9 to 26, and the phenotypic variations explained by each marker varied from 12.07% to 31.32%. Apart from five previously reported markers, the remaining 38 markers were newly reported here. Twenty-one markers were identified as simultaneously linked to two to four traits, and five markers were associated with the same traits in cultivation tests performed in two consecutive years. The 43 traits-associated markers were related to 97 genes, and 24 of them were related to 10 traits-associated markers detected in both years or identified previously, 13 of which had a >2-fold expression change between the mycelium and primordium stages. Our study has provided candidate markers for marker-assisted selection (MAS) and useful clues for understanding the genetic architecture of agronomic traits in the shiitake mushroom.
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University Hubei, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural UniversityHubei, China; Institute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesChangsha, China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Gong
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University Hubei, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Informatics, Huazhong Agricultural University Hubei, China.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong Hong Kong, Hong Kong.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Nong
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University Hubei, China.
authors.forename: Yinbing
authors.initials: Y
authors.lastname: Bian
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong Hong Kong, Hong Kong.
authors.forename: Hoi-Shan
authors.initials: HS
authors.lastname: Kwan
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong Hong Kong, Hong Kong.
authors.forename: Man-Kit
authors.initials: MK
authors.lastname: Cheung
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University Hubei, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
keywords: InDel; association analysis; microsatellite; quantitative trait; shiitake mushroom