title: Histopathological assessment of OASIS Ultra on critical-sized wound healing: a pilot study.
abstract: BACKGROUND
  Dermatopathologists assess wounds secondary to trauma, infection, or oncologic resection that can be challenging to reconstruct. OASIS Ultra, an extracellular matrix, has been described for use in chronic and burn wounds. The aim of this pilot study is to assess wound healing in post-traumatic and infective wounds treated with OASIS using histological markers of repair.
  
  
  METHODS
  Adults with traumatic, infective or iatrogenic wound defects with size precluding primary closure were eligible. Half the wound was randomly assigned to receive OASIS plus standard therapy; the other half received standard of care (SOC) therapy. During dressing changes, standardized-scale photographs were taken and biopsies obtained. Histologic sections were reviewed for degree of acute inflammation and extent of tissue repair. Neutrophils, edema, hemorrhage, necrosis, fibroblasts, collagen density and neovascularization were semi-quantitatively assessed.
  
  
  RESULTS
  Forty-four skin biopsies from 7 patients with 10 acute wounds met eligibility criteria. Histologically, OASIS samples demonstrated improved acute inflammation scores compared to SOC. No patients experienced OASIS-related complications. OASIS-treated wound halves trended toward more wound contraction and improved tissue repair.
  
  
  CONCLUSIONS
  Our scoring system aids histopathological wound assessment. Treatment of critical-sized, post-traumatic, acute wounds with OASIS resulted in decreased inflammation, and potentially more advanced wound healing, compared to SOC.
authors.affiliation: Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Boston, Massachusetts.|Harvard Medical School, Boston, Massachusetts.
authors.forename: Daniel Dante
authors.initials: DD
authors.lastname: Yeh
authors.affiliation: Harvard Medical School, Boston, Massachusetts.|Department of Pathology, Dermatopathology Unit, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Rosalynn M
authors.identifier: http://orcid.org/0000-0003-4003-7193
authors.initials: RM
authors.lastname: Nazarian
authors.affiliation: Harvard Medical School, Boston, Massachusetts.
authors.forename: Leah
authors.initials: L
authors.lastname: Demetri
authors.affiliation: Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Boston, Massachusetts.|Harvard Medical School, Boston, Massachusetts.
authors.forename: Tomaz
authors.initials: T
authors.lastname: Mesar
authors.affiliation: Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Suzan
authors.initials: S
authors.lastname: Dijkink
authors.affiliation: Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Boston, Massachusetts.|Harvard Medical School, Boston, Massachusetts.
authors.forename: Andreas
authors.initials: A
authors.lastname: Larentzakis
authors.affiliation: Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Boston, Massachusetts.|Harvard Medical School, Boston, Massachusetts.
authors.forename: George
authors.initials: G
authors.lastname: Velmahos
authors.affiliation: Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Boston, Massachusetts.|Harvard Medical School, Boston, Massachusetts.
authors.forename: Karim Walid
authors.initials: KW
authors.lastname: Sadik
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001458:Bandages; D001706:Biopsy; D002056:Burns / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D002908:Chronic Disease; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D010865:Pilot Projects; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy
keywords: OASIS; acute inflammation; extracellular matrix; fibrosis; wound healing