title: Unveiling the in Vivo Protein Corona of Circulating Leukocyte-like Carriers.
abstract: Understanding interactions occurring at the interface between nanoparticles and biological components is an urgent challenge in nanomedicine due to their effect on the biological fate of nanoparticles. After the systemic injection of nanoparticles, a protein corona constructed by blood components surrounds the carrier's surface and modulates its pharmacokinetics and biodistribution. Biomimicry-based approaches in nanotechnology attempt to imitate what happens in nature in order to transfer specific natural functionalities to synthetic nanoparticles. Several biomimetic formulations have been developed, showing superior in vivo features as a result of their cell-like identity. We have recently designed biomimetic liposomes, called leukosomes, which recapitulate the ability of leukocytes to target inflamed endothelium and escape clearance by the immune system. To gain insight into the properties of leukosomes, we decided to investigate their protein corona in vivo. So far, most information about the protein corona has been obtained using in vitro experiments, which have been shown to minimally reproduce in vivo phenomena. Here we directly show a time-dependent quantitative and qualitative analysis of the protein corona adsorbed in vivo on leukosomes and control liposomes. We observed that leukosomes absorb fewer proteins than liposomes, and we identified a group of proteins specifically adsorbed on leukosomes. Moreover, we hypothesize that the presence of macrophage receptors on leukosomes' surface neutralizes their protein corona-meditated uptake by immune cells. This work unveils the protein corona of a biomimetic carrier and is one of the few studies on the corona performed in vivo.
authors.affiliation: CEINGE-Biotecnologie Avanzate s.c.a r.l. , Via G. Salvatore 486, Naples, 80145, Italy.
authors.forename: Claudia
authors.identifier: 0000-0001-6468-2571
authors.initials: C
authors.lastname: Corbo
authors.forename: Roberto
authors.initials: R
authors.lastname: Molinaro
authors.forename: Francesca
authors.identifier: 0000-0002-4959-1169
authors.initials: F
authors.lastname: Taraballi
authors.forename: Naama E
authors.initials: NE
authors.lastname: Toledano Furman
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Hartman
authors.affiliation: Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch , Galveston, Texas 77555, United States.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Sherman
authors.forename: Enrica
authors.initials: E
authors.lastname: De Rosa
authors.forename: Dickson K
authors.initials: DK
authors.lastname: Kirui
authors.affiliation: CEINGE-Biotecnologie Avanzate s.c.a r.l. , Via G. Salvatore 486, Naples, 80145, Italy.|Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II , Via Sergio Pansini 5, Naples, 80131, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Salvatore
authors.affiliation: Department of Orthopedics and Sports Medicine, Houston Methodist Hospital , 6565 Fannin Street, Houston, Texas 77030, United States.
authors.forename: Ennio
authors.identifier: 0000-0003-1187-3205
authors.initials: E
authors.lastname: Tasciotti
mesh_terms: D000327:Adsorption; D000818:Animals; D002460:Cell Line; D020285:Cryoelectron Microscopy; D019075:Electrophoresis, Capillary; D008081:Liposomes / Q000097:blood / Q000737:chemistry; D013058:Mass Spectrometry; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D000066970:Protein Corona / Q000737:chemistry*
chemical_list: D008081:Liposomes; D000066970:Protein Corona
keywords: biomimicry; in vivo corona; leukosome; liposome; macrophages; nanomedicine; protein corona