title: Production of Polyhydroxyalkanoates from Sludge Palm Oil UsingPseudomonas putidaS12.
abstract: Polyhydroxyalkanoates (PHAs) are biodegradable plastics produced by bacteria, but their use in diverse applications is prohibited by high production costs. To reduce these costs, the conversion byPseudomonasstrains of P HAs from crude s ludge p alm oil ( SPO) a s an inexpensive renewable raw material was tested.Pseudomonas putidaS12 was found to produce the highest yield (~41%) of elastomeric medium-chain-length (MCL)-PHAs from SPO. The MCL-PHA characteristics were analyzed by gas-chromatography/mass spectrometry, gel permeation chromatography, and differential scanning calorimetry. These findings may contribute to more widespread use of PHAs by reducing PHA production costs.
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.|Department of Biosystems and Bioengineering, Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
authors.forename: Du-Kyeong
authors.initials: DK
authors.lastname: Kang
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.|Department of Biosystems and Bioengineering, Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
authors.forename: Cho-Ryong
authors.initials: CR
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Molecular Biology, Chungnam National Univercity, Daejeon 34134, Republic of Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Bae
authors.affiliation: School of Environmental Engineering, University of Seoul, Seoul 02504, Republic of Korea.
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Park
authors.affiliation: Department of Microbiology and Molecular Biology, Chungnam National Univercity, Daejeon 34134, Republic of Korea.
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Rhee
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.|Department of Biosystems and Bioengineering, Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Sung
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.|Department of Biosystems and Bioengineering, Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Sohn
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D000073878:Palm Oil; D010938:Plant Oils / Q000032:analysis / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry*
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D010938:Plant Oils; D054813:Polyhydroxyalkanoates; D012722:Sewage; D000073878:Palm Oil
keywords: PHA; Pseudomonas; Sludge palm oil; bioplastic; waste utilization