title: Genetic characterization of T-DNA insertions in the genome of the Arabidopsis thaliana sumo1/2 knock-down line.
abstract: Sumoylation is an essential post-translational modification in Arabidopsis thaliana, which entails the conjugation of the SUMO protein onto lysine residues in target proteins. In Arabidopsis, 2 closely related genes, SUMO1 and SUMO2, act redundantly and are in combination essential for plant development, i.e. the combined loss of SUMO1 and SUMO2 results in embryo-lethality. To circumvent this lethality, SUMO2 was previously knocked down in a sumo1 knockout background by expressing an artificial microRNA that targets SUMO2 (amiR-SUMO2). This sumo1/2KDline with low SUMO2 levels represents a valuable genetics tool to investigate SUMO function in planta. Here, we re-sequenced the whole-genome of this sumo1/2KDline and identified 2 amiR-SUMO2 insertions in this line, which were confirmed by PCR-genotyping. Identification of these 2 insertions enables genetics with this tool.
authors.affiliation: a Molecular Plant Pathology , Swammerdam Institute for Life Sciences, University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: Valentin
authors.initials: V
authors.lastname: Hammoudi
authors.affiliation: a Molecular Plant Pathology , Swammerdam Institute for Life Sciences, University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: Georgios
authors.initials: G
authors.lastname: Vlachakis
authors.affiliation: b VIB Department of Plant Systems Biology , Ghent University , Belgium.
authors.forename: Ronnie
authors.initials: R
authors.lastname: de Jonge
authors.affiliation: c RNA Biology & Applied Bioinformatics , University of Amsterdam , the Netherlands.
authors.forename: Timo M
authors.initials: TM
authors.lastname: Breit
authors.affiliation: a Molecular Plant Pathology , Swammerdam Institute for Life Sciences, University of Amsterdam , Amsterdam , the Netherlands.
authors.forename: Harrold A
authors.initials: HA
authors.lastname: van den Burg
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011499:Protein Processing, Post-Translational / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D035683:MicroRNAs; C036483:T-DNA
keywords: Arabidopsis; SUMO; T-DNA insertions; whole genome resequencing