title: [Identification of HLA class Ⅱ susceptible alleles and genotypes in latent autoimmune diabetes in adults].
abstract: Objective:To identify human leukocyte antigen (HLA)-DRB1, DQA1 and DQB1 susceptible alleles and genotypes in latent autoimmune diabetes in adults (LADA) patients of Chinese Han nationality.Methods:All subjects including 652 LADA patients and 1 181 healthy controls from 1999 to 2015 in Han nationality region of Hunan province were genotyped with high resolution at HLA-DRB1, DQA1 and DQB1 locus by PCR-sequence based typing (PCR-SBT). Frequencies of genotypes between patients and controls were compared by chi square test.Results:The DQA1 susceptible allele was DQA1*03 (OR=1.23,P(c)=0.028); the DQB1 susceptible alleles were DQB1*0201 (OR=2.24,P(c)<0.001), DQB1*0303 (OR=1.30,P(c)=0.030), DQB1*0304 (OR=10.23,P(c)=0.004) and DQB1*0401(OR=1.94,P(c)<0.001); and the DRB1 susceptible alleles were DRB1*0301 (OR=2.10,P(c)<0.001), DRB1*0405(OR=1.89,P(c)<0.001) and DRB1*0901(OR=1.36,P(c)=0.008), respectively in Chinese Han nationality LADA patients. The HLA-Ⅱ susceptible genotypes were DQA1*03/05 (OR=1.81,P(c)=0.007), DQB1*0201/0201(OR=5.74,P(c)<0.001), DQB1*0201/0303 (OR=2.58,P(c)=0.010), DRB1*0301/0901(OR=3.43,P(c)=0.028) and DRB1*0901/0901 (OR=1.82,P(c)=0.021), respectively in LADA patients. DQB1*0201 and DRB1*0301 were shared susceptible alleles for Chinese Han and Caucasian LADA patients, while DQA1*03, DQB1*0303, DQB1*0304, DQB1*0401, DRB1*0405 and DRB1*0901 were specific susceptible alleles for Chinese Han LADA patients. Interestingly, the DQB1*0303 allele was susceptible in Chinese while protective in Caucasian (OR:1.30 vs 0.29).Conclusion:Susceptible alleles are DQA1*03, DQB1*0201, DQB1*0303, DQB1*0304, DQB1*0401, DRB1*0301, DRB1*0405 and DRB1*0901, and susceptible genotypes are DQA1*03/05, DQB1*0201/0201, DQB1*0201/0303, DRB1*0301/0901 and DRB1*0901/0901 in Chinese LADA patients.
authors.affiliation: Department of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Key Laboratory of Diabetes Immunology, Ministry of Education, National Clinical Research Center for Metabolic Diseases, Changsha 410011, China.
authors.forename: N N
authors.initials: NN
authors.lastname: Yin
authors.forename: S M
authors.initials: SM
authors.lastname: Luo
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: Z G
authors.initials: ZG
authors.lastname: Xie
authors.forename: G
authors.initials: G
authors.lastname: Huang
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: P L
authors.initials: PL
authors.lastname: Zheng
authors.forename: J P
authors.initials: JP
authors.lastname: Wang
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People; D003922:Diabetes Mellitus, Type 1; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059866:HLA-DQ beta-Chains; D059811:HLA-DRB1 Chains; D006801:Humans; D000071698:Latent Autoimmune Diabetes in Adults; D044465:White People
chemical_list: D059866:HLA-DQ beta-Chains; D059811:HLA-DRB1 Chains
keywords: Alleles; Autoimmune diseases; Diabetes mellitus, type 1; Genotype; HLA