title: Assessment ofALKgene rearrangement in lung cancer using a new rapid automated SureFISH (Dako Omnis) assay.
abstract: TheALKfluorescence in situ hybridisation (FISH) method is the examination essential for pathological diagnosis and choice of molecular-targeted therapy inALK-rearranged lung cancer. Here, for detection ofALKgene rearrangement in patients with lung cancer, we evaluated the rapid FISH technology (ALKSureFISH), a newly developed assay for the automated staining platform Dako Omnis, using 21 formalin-fixed paraffin-embedded (FFPE) samples. All cases could be evaluated with the SureFISH method. SureFISH provided excellent quality signals without any background staining. The SureFISH assay was able to offer a rapid turnaround time (approximately 3.5 hours) and was 100% concordant with prior Vysis FISH results in our laboratory.
authors.affiliation: Department of Laboratory Medicine and Pathology, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Takeda
authors.affiliation: Department of Laboratory Medicine and Pathology, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Kasai
authors.affiliation: Department of Pathology, Kinki University of Medicine, Osaka-Sayama, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Shimizu
authors.affiliation: Department of Laboratory Medicine and Pathology, National Hospital Organization Minami Wakayama Medical Center, Wakayama, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Kitaichi
authors.affiliation: Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kojima
authors.affiliation: Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nagoya
authors.affiliation: Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Hayashi
authors.affiliation: Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Utsumi
authors.affiliation: Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Hyung-Eun
authors.initials: HE
authors.lastname: Yoon
authors.affiliation: Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Akihide
authors.initials: A
authors.lastname: Matsumura
authors.affiliation: Department of Thoracic Oncology, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Atagi
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000077548:Anaplastic Lymphoma Kinase; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases
keywords: FISH; LUNG CANCER; MOLECULAR PATHOLOGY