title: Size-Tunable Gd2O3@Albumin Nanoparticles Conjugating Chlorin e6 for Magnetic Resonance Imaging-Guided Photo-Induced Therapy.
abstract: Protein nanoparticles as nanocarriers are of particular interest in the field of cancer therapy. Nevertheless, so far a facile fabrication of theranostic protein nanoparticles have been explored with limited success for cancer imaging and therapy. In this work, we demonstrate the controllable synthesis of size-tunable Gd2O3@albumin conjugating photosensitizer (PS) (GA-NPs) using hollow albumin as the nanoreactor for magnetic resonance imaging (MRI)-guided photo-induced therapy. The growth of Gd2O3nanocrystals within the hollow nanoreactors is well regulated through reaction time, and a typical PS (e.g. chlorin e6) is further conjugated with the protein corona of the nanoreactor through facile chemical coupling, followed by the formation of theranostic GA-NPs. GA-NPs exhibit good longitudinal relaxivity, ideal photostability, enhanced cellular uptakes, and preferable size-dependent tumor accumulation. Moreover, GA-NPs effectively generate remarkable photothermal effect, intracellular reactive oxygen species from Ce6, and subsequent cytoplasmic drug translocation, thereby leading to severe synergistic photothermal and photodynamic cell damages. Consequently, GA-NPs exhibit anin vivosize-dependent MRI capacity with enhanced imaging contrast for effective tumor localization, and also generate a potent synergistic photodynamic therapy/photothermal therapy efficacy under irradiation owing to their enhanced tumor accumulation and strong photo-induced cytotoxicity. These results suggest that GA-NPs can act as a promising theranostic protein nanoplatform for cancer imaging and photo-induced therapy.
authors.affiliation: Department of Radiology, Second Affiliated Hospital of Soochow University, Suzhou215004, Jiangsu, China;; Institute of Radiotherapy & Oncology, Soochow University, Suzhou 215004, Jiangsu, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, and College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Radiology, Second Affiliated Hospital of Soochow University, Suzhou215004, Jiangsu, China.
authors.forename: Junxing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, and College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, and College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Lv
authors.affiliation: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, and College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Hengte
authors.initials: H
authors.lastname: Ke
authors.affiliation: School for Radiological & Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, and School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Zhengqing
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Radiology, Second Affiliated Hospital of Soochow University, Suzhou215004, Jiangsu, China;; Institute of Radiotherapy & Oncology, Soochow University, Suzhou 215004, Jiangsu, China.
authors.forename: Junkang
authors.initials: J
authors.lastname: Shen
authors.affiliation: School for Radiological & Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, and School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China.
authors.forename: Chungen
authors.initials: C
authors.lastname: Xing
authors.affiliation: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, and College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, Jiangsu, China;; School for Radiological & Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, and School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Huabing
authors.initials: H
authors.lastname: Chen
mesh_terms: D000418:Albumins / Q000008:administration & dosage*; D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002735:Chlorophyllides; D005682:Gadolinium / Q000008:administration & dosage*; D006979:Hyperthermia, Induced / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011166:Porphyrins / Q000008:administration & dosage*; D000068936:Theranostic Nanomedicine / Q000379:methods; D016896:Treatment Outcome
chemical_list: D000418:Albumins; D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; C030581:gadolinium oxide; C062985:phytochlorin; D005682:Gadolinium
keywords: albumin nanoreactor; gadolinium oxide; magnetic resonance imaging; photodynamic therapy.; photosensitizer