title: [Investigation of the surface layer of 3D-matrices for tissue engineering].
abstract: Electrospinning is a convenient and promising manufacturing method a variety of materials for tissue engineering. 3D matrices fabricated by electrospinning from solutions of polycaprolactone with human serum albumin or gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The microstructure of the 3D matrices and surface of the fibers were investigated using scanning electron microscopy. Protein distribution in the surface layer was studied by modification of protein amino groups with N-(2-hydroxyethyl)phenazine and X-ray photoelectron spectroscopy. It was shown, that concentration of the proteins in the surface layer of fibers exceeded their concentration in the initial electrospun solution up to 12 times and the surface layer was enriched in the protein inversely to the concentration of the protein in solution. The minor part of the proteins was released from fibers during first 30-60 min after swelling in water. Treatment of matrices with proteinase K hydrolyzed about 1/3 of the surface exposed human serum albumin. Thus, both methods can be used to study the surface content of the materials produced by electrospinning from blends of synthetic and natural polymers, however X-ray photoelectron spectroscopy appears to be more convenient and informative.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Russian Academy of Science, Novosibirsk, Russia; Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Chernonosova
authors.affiliation: Boreskov Institute of Catalysis Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: R I
authors.initials: RI
authors.lastname: Kvon
authors.affiliation: Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Kiseleva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Russian Academy of Science, Novosibirsk, Russia; Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia.
authors.forename: A O
authors.initials: AO
authors.lastname: Stepanova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Russian Academy of Science, Novosibirsk, Russia; Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia.
authors.forename: P P
authors.initials: PP
authors.lastname: Laktionov
mesh_terms: D055664:Electrochemical Techniques; D019286:Endopeptidase K / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010619:Phenazines / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D020005:Propanols / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D006845:Hydrocarbons, Fluorinated
chemical_list: D019286:Endopeptidase K; D010619:Phenazines; D011091:Polyesters; D020005:Propanols; D012709:Serum Albumin; C076555:10-(2-hydroxyethyl)phenazine; C016240:polycaprolactone; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated
keywords: 3D-matrices; X-ray photoelectron spectroscopy; chemical modification; electrospinning; synthetic and natural polymers