title: Bakery by-products based feeds borne-Saccharomyces cerevisiae strains with probiotic and antimycotoxin effects plus antibiotic resistance properties for use in animal production.
abstract: The aim of this study was to select S. cerevisiae strains able to exert probiotic and antimycotoxin effects plus antibiotics resistance properties for use in animal production. S. cerevisiae LL74 and S. cerevisiae LL83 were isolated from bakery by-products intended for use in animal feed and examined for phenotypic characteristics and nutritional profile. Resistance to antibiotic, tolerance to gastrointestinal conditions, autoaggregation and coaggregation assay, antagonism to animal pathogens and aflatoxin B1binding were studied. S. cerevisiae LL74 and S. cerevisiae LL83 showed resistance to all the antibiotics assayed (ampicillin, streptomycin, neomycin, norfloxacin, penicillin G, sulfonamide and trimethoprim). The analysis showed that exposure time to acid pH had a significant impact onto the viable cell counts onto both yeast strains. Presence of bile 0.5% increased significantly the growth of the both yeast strains. Moreover, they were able to tolerate the simulated gastrointestinal conditions assayed. In general, the coaggregation was positive whereas the autoaggregation capacity was not observed. Both strains were able to adsorb AFB1. In conclusion, selected S. cerevisiae LL74 and S. cerevisiae LL83 have potential application to be used as a biological method in animal feed as antibiotic therapy replacement in, reducing the adverse effects of AFB1and giving probiotic properties.
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km.601. (5800), Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina.
authors.forename: Valeria
authors.initials: V
authors.lastname: Poloni
authors.affiliation: Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Avenida Antonio Carlos 6627, Pampulha, 31270901, Belo Horizonte, MG, Brazil.
authors.forename: Lauranne
authors.initials: L
authors.lastname: Salvato
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km.601. (5800), Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina.
authors.forename: Carina
authors.initials: C
authors.lastname: Pereyra
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Rod. BR465 Km 7, Seropédica, Rio de Janeiro, Brazil.
authors.forename: Aguida
authors.initials: A
authors.lastname: Oliveira
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Rod. BR465 Km 7, Seropédica, Rio de Janeiro, Brazil; Member of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Rosa
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km.601. (5800), Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina. Electronic address: lcavaglieri@exa.unrc.edu.ar.
authors.forename: Lilia
authors.initials: L
authors.lastname: Cavaglieri
authors.affiliation: Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Avenida Antonio Carlos 6627, Pampulha, 31270901, Belo Horizonte, MG, Brazil.
authors.forename: Kelly Moura
authors.initials: KM
authors.lastname: Keller
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D009183:Mycotoxins / Q000494:pharmacology*; D019936:Probiotics / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology; D014866:Waste Products / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D009183:Mycotoxins; D014866:Waste Products
keywords: Adsorption; Aflatoxin B(1); Antibiotic resistance; Saccharomyces cerevisiae