title: Identification and validation of sRNAs in Edwardsiella tarda S08.
abstract: Bacterial small non-coding RNAs (sRNAs) are known as novel regulators involved in virulence, stress responsibility, and so on. Recently, a lot of new researches have highlighted the critical roles of sRNAs in fine-tune gene regulation in both prokaryotes and eukaryotes. Edwardsiella tarda (E. tarda) is a gram-negative, intracellular pathogen that causes edwardsiellosis in fish. Thus far, no sRNA has been reported in E. tarda. The present study represents the first attempt to identify sRNAs in E. tarda S08. Ten sRNAs were validated by RNA sequencing and quantitative PCR (qPCR). ET_sRNA_1 and ET_sRNA_2 were homolous to tmRNA and GcvB, respectively. However, the other candidate sRNAs have not been reported till now. The cellular abundance of 10 validated sRNA was detected by qPCR at different growth phases to monitor their biosynthesis. Nine candidate sRNAs were expressed in the late-stage of exponential growth and stationary stages of growth (36~60 h). And the expression of the nine sRNAs was growth phase-dependent. But ET_sRNA_10 was almost expressed all the time and reached the highest peak at 48 h. Their targets were predicted by TargetRNA2 and each sRNA target contains some genes that directly or indirectly relate to virulence. These results preliminary showed that sRNAs probably play a regulatory role of virulence in E. tarda.
authors.affiliation: College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang, China.|Jiangsu Marine Resources Development Research Institute, Lianyungang, China.|Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Jiquan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.affiliation: College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Yan
authors.affiliation: College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D020609:Edwardsiella tarda / Q000235:genetics* / Q000302:isolation & purification; D005412:Flatfishes / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D058727:RNA, Small Untranslated / Q000737:chemistry / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014774:Virulence / Q000235:genetics
chemical_list: D058727:RNA, Small Untranslated