title: Molecular characterization and biological activity of bovine interferon-omega3.
abstract: Bovine interferon-omega3 (BoIFN-ω3) gene was amplified from bovine liver genomic DNA, which encodes a 195-amino acid protein containing a 23-amino acid signal peptide. Analysis of the molecular characteristics revealed that BoIFN-ω3 evolving from IFN-ω, contained four cysteine residues and five alpha helices, showing that BoIFN-ω3 presented the typical molecular characteristics of type I interferon. BoIFN-ω3 exhibited antiviral and antiproliferative activities, which exerted a protective effect against VSV in several mammalian cell lines, as well as against BEV, IBRV, and BVDV in MDBK cell. Moreover, BoIFN-ω3 was shown to be highly sensitive to trypsin, but remaining stable despite changes in pH and temperature. Additionally, BoIFN-ω3 induced the transcription of Mx1, ISG15, and ISG56 genes, as well as the expression of Mx1 protein in a time-dependent manner. These findings will be useful to further study BoIFN-ω in host's defence against infectious diseases, particularly viral infections. Furthermore, results will facilitate further research on the bovine interferon family.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: An
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China; National Food Safety and Nutrition Collaborative Innovation Center, Wuxi, Jiangsu 214122, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Xiuxin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China. Electronic address: gaomingchun@163.com.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China; National Food Safety and Nutrition Collaborative Innovation Center, Wuxi, Jiangsu 214122, PR China. Electronic address: jwwang@neau.edu.cn.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002417:Cattle / Q000502:physiology*; D002460:Cell Line; D004247:DNA; D007370:Interferon Type I / Q000737:chemistry* / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D014780:Viruses / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D004247:DNA
keywords: Antiviral activity; Bovine; IFN-ω3; Interferon-stimulated genes; Molecular characteristic