title: SNP-SNP interactions between WNT4 and WNT5A were associated with obesity related traits in Han Chinese Population.
abstract: Considering the biological roles of WNT4 and WNT5A involved in adipogenesis, we aimed to investigate whether SNPs in WNT4 and WNT5A contribute to obesity related traits in Han Chinese population. Targeted genomic sequence for WNT4 and WNT5A was determined in 100 Han Chinese subjects and tag SNPs were selected. Both single SNP and SNP × SNP interaction association analyses with body mass index (BMI) were evaluated in the 100 subjects and another independent sample of 1,627 Han Chinese subjects. Meta-analyses were performed and multiple testing corrections were carried out using the Bonferroni method. Consistent with the Genetic Investigation of ANthropometric Traits (GIANT) dataset results, we didn't detect significant association signals in single SNP association analyses. However, the interaction between rs2072920 and rs11918967, was associated with BMI after multiple testing corrections (combined P = 2.20 × 10-4). The signal was also significant in each contributing data set. SNP rs2072920 is located in the 3'-UTR of WNT4 and SNP rs11918967 is located in the intron of WNT5A. Functional annotation results revealed that both SNPs might be involved in transcriptional regulation of gene expression. Our results suggest that a combined effect of SNPs via WNT4-WNT5A interaction may affect the variation of BMI in Han Chinese population.
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Dong
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors.forename: Tie-Lin
authors.initials: TL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Yan
authors.affiliation: Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha 410081, P. R. China.
authors.forename: Xiang-Ding
authors.initials: XD
authors.lastname: Chen
authors.affiliation: Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha 410081, P. R. China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Tan
authors.affiliation: School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tian
authors.affiliation: School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
authors.forename: Hong-Wen
authors.initials: HW
authors.lastname: Deng
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D015992:Body Mass Index; D005260:Female; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D007438:Introns; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics / Q000473:pathology*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D000071818:Wnt-5a Protein / Q000235:genetics*; D060528:Wnt4 Protein / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C555581:WNT4 protein, human; C085389:WNT5A protein, human; D000071818:Wnt-5a Protein; D060528:Wnt4 Protein