title: Qualitative and quantitative evaluation ofin vivoSD-OCT measurement of rat brain.
abstract: OCT has been demonstrated as an efficient imaging modality in various biomedical and clinical applications. However, there is a missing link with respect to the source of contrast between OCT and other modern imaging modalities, no quantitative comparison has been demonstrated between them, yet. We evaluated, to our knowledge, for the first timein vivoOCT measurement of rat brain with our previously proposed forward imaging method by both qualitatively and quantitatively correlating OCT with the corresponding T1-weighted and T2-weighted magnetic resonance images, fiber density map (FDM), and two types of histology staining (cresyl violet and acetylcholinesterase AchE), respectively. Brain anatomical structures were identified and compared across OCT, MRI and histology imaging modalities. Noticeable resemblances corresponding to certain anatomical structures were found between OCT and other image profiles. Correlation was quantitatively assessed by estimating correlation coefficient (R) and mutual information (MI). Results show that the 1-D OCT measurements in regards to the intensity profile and estimated attenuation factor, do not have profound linear correlation with the other image modalities suggested from correlation coefficient estimation. However, findings in mutual information analysis demonstrate that there are markedly high MI values in OCT-MRI signals.
authors.affiliation: Section of Neuroelectronic Systems, Department of General Neurosurgery, Medical Center University of Freiburg, Engesserstraβe 4, 79108 Freiburg, Germany; Translational Imaging Group, Centre for Medical Image Computing, Department of Medical Physics and Biomedical Engineering, University College London, WC1E 6BT London, UK.
authors.forename: Yijing
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Diagnostic Radiology, Medical Center University of Freiburg, Hugstetter Straβe 55, 79106 Freiburg, Germany; Laboratory of Engineering, Informatics and Imaging (ICube), Integrative Multimodal Imaging in Healthcare (IMIS), UMR 7357, University of Strasbourg, 4 Rue Kirschleger, 67000 Strasbourg, France; Department of Biophysics and Nuclear Medicine, Hautepierre Hospital, University Hospitals of Strasbourg, 1, Avenue Molière, 67098 Strasbourg Cedex, France.
authors.forename: Laura-Adela
authors.initials: LA
authors.lastname: Harsan
authors.affiliation: Department of Diagnostic Radiology, Medical Center University of Freiburg, Hugstetter Straβe 55, 79106 Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bienert
authors.affiliation: Section of Neuroelectronic Systems, Department of General Neurosurgery, Medical Center University of Freiburg, Engesserstraβe 4, 79108 Freiburg, Germany.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Kirch
authors.affiliation: Department of Diagnostic Radiology, Medical Center University of Freiburg, Hugstetter Straβe 55, 79106 Freiburg, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: von Elverfeldt
authors.affiliation: Section of Neuroelectronic Systems, Department of General Neurosurgery, Medical Center University of Freiburg, Engesserstraβe 4, 79108 Freiburg, Germany; Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg and University of Strasbourg Institute for Advanced Study (USIAS), Strasbourg, France.
authors.forename: Ulrich G
authors.initials: UG
authors.lastname: Hofmann
keywords: (170.0170) Medical optics and biotechnology; (170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography