title: A combination of GDNF and hUCMSC transplantation loaded on SF/AGs composite scaffolds for spinal cord injury repair.
abstract: Spinal cord injury (SCI) is a severe trauma for which no effective treatment is currently available. In this study, a composited treatment system was prepared using a silk fibroin/alginates/glial cell line-derived neurotrophic factor (SF/AGs/GDNF) scaffold seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) and the combined therapeutic effects of the composite scaffold to repair SCI rats were evaluated. The use of SF as a scaffold material could act as a biomimetic platform allowing neurons to properly accommodate and rebuild the target tissue. The SF/AGs/GDNF scaffold had the best sustained-release function and the AGs were the key determining factor in the controlled release of GDNF. After 8weeks of treatment, the hUCMSCs on SF/AGs/GDNF composite scaffolds could significantly enhance the scar expansion of spinal cord tissue and increased the number of surviving neurons. The combination of GDNF and hUCMSCs transplantation loaded on SF/AGs composite scaffolds exhibited better therapeutic and repair effects to the SCI of rats, compared with the SF/AGs group or GDNF alone on SF/AGs scaffolds. The composite scaffold, GDNF and stem cells could build a bioactive material to form the micro-environment of growth and repair of the neurons. These results may provide a theoretical basis and beneficial exploration for clinical treatment of SCI.
authors.affiliation: Department of Orthopedic, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
authors.forename: Genlong
authors.initials: G
authors.lastname: Jiao
authors.affiliation: Institute of Biomedicine & Department of Cell Biology, Jinan University, Guangzhou 510632, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Lou
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China; Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Mo
authors.affiliation: Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Ziyong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China; Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China. Electronic address: guorui@jnu.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Orthopedic, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China. Electronic address: lizhizhong1@medmail.com.cn.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001522:Behavior, Animal; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016716:PC12 Cells; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D000464:Alginates; D051100:Glial Cell Line-Derived Neurotrophic Factor
keywords: Alginate microspheres; Glial cell-derived neurotrophic factor; Human umbilical cord mesenchymal stem cells; Scaffold; Silk fibroin; Spinal cord injury