title: Methods to investigate the structure and connectivity of the nervous system.
abstract: Understanding the computations that take place in neural circuits requires identifying how neurons in those circuits are connected to one another. In addition, recent research indicates that aberrant neuronal wiring may be the cause of several neurodevelopmental disorders, further emphasizing the importance of identifying the wiring diagrams of brain circuits. To address this issue, several new approaches have been recently developed. In this review, we describe several methods that are currently available to investigate the structure and connectivity of the brain, and discuss their strengths and limitations.
authors.affiliation: a Division of Biology and Biological Engineering , California Institute of Technology , Pasadena , CA , USA.
authors.forename: Donghyung
authors.initials: D
authors.lastname: Lee
authors.affiliation: a Division of Biology and Biological Engineering , California Institute of Technology , Pasadena , CA , USA.
authors.forename: Ting-Hao
authors.initials: TH
authors.lastname: Huang
authors.affiliation: a Division of Biology and Biological Engineering , California Institute of Technology , Pasadena , CA , USA.
authors.forename: Aubrie
authors.initials: A
authors.lastname: De La Cruz
authors.affiliation: a Division of Biology and Biological Engineering , California Institute of Technology , Pasadena , CA , USA.|b Department of Cell Biology, School of Science , University of Extremadura , Badajoz , Spain.
authors.forename: Antuca
authors.initials: A
authors.lastname: Callejas
authors.affiliation: a Division of Biology and Biological Engineering , California Institute of Technology , Pasadena , CA , USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Lois
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure*; D020869:Gene Expression Profiling / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D009415:Nerve Net / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure
keywords: brain circuit; connectomics; neuron; synapse; wiring diagram