title: Isolation of Cryptococcus gattii from a Castanopsis argyrophylla tree hollow (Mai-Kaw), Chiang Mai, Thailand.
abstract: The pathogenic yeast Cryptococcus gattii was isolated from a tree hollow of a Castanopsis argyrophylla King ex Hook.f. (Fagaceae) in Chiang Mai, Thailand. Molecular characterization with amplified fragment length polymorphism analysis and multi-locus sequence typing showed that this isolate belonged to genotype AFLP4/VGI representing C. gattii sensu stricto. Subsequent comparison of the environmental isolate with those from clinical samples from Thailand showed that they grouped closely together in a single cluster.
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Medical Sciences, University of Phayao, 19 M2 Maeka, Muang, Phayao, 56000, Thailand. kantarawee.kh@up.ac.th.|CBS-KNAW Fungal Biodiversity Centre, Basidiomycete and Yeast Research, Utrecht, The Netherlands. kantarawee.kh@up.ac.th.
authors.forename: Kantarawee
authors.initials: K
authors.lastname: Khayhan
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands.
authors.forename: Ferry
authors.initials: F
authors.lastname: Hagen
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Treepradab
authors.initials: T
authors.lastname: Norkaew
authors.affiliation: Queen Sirikit Botanic Garden, Chiang Mai, Thailand.
authors.forename: Tanpalang
authors.initials: T
authors.lastname: Puengchan
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Basidiomycete and Yeast Research, Utrecht, The Netherlands.|Institute of Biodiversity and Ecosystem Dynamics (IBEA), University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Teun
authors.initials: T
authors.lastname: Boekhout
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Pojana
authors.initials: P
authors.lastname: Sriburee
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D016000:Cluster Analysis; D056285:Cryptococcus gattii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D029962:Fagaceae / Q000382:microbiology*; D005838:Genotype; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D013785:Thailand; D014197:Trees / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal
keywords: AFLP genotyping; Cryptococcus gattii; Environmental sampling; Multi-locus sequence typing; Thailand