title: Reversible Light-Switching of Enzymatic Activity on Orthogonally Functionalized Polymer Brushes.
abstract: Copolymer brushes, composed of glycidyl methacrylate and a furan-protected maleimide-containing monomer, were grafted from radical initiators at the surface of irradiation-activated fluoropolymer foils. After postpolymerization modification with enzymatically active microperoxidase-11 and photochromic spiropyran moieties, the polymer brushes catalyzed the oxidation of 3,3'5,5'-tetramethylbenzidine. Exposure to either UV or visible-light allowed switching the turnover by more than 1 order of magnitude, as consequence of the reversible, light-induced spiropyran-merocyanine transition. The modified samples were integrated into an optofluidic device that allowed the reversible switching of enzymatic activity for several cycles under flow, validating the potential for application in smart lab-on-a-chip systems.
authors.affiliation: Laboratory for Micro- and Nanotechnology, Paul Scherrer Institute , 5232 Villigen PSI, Switzerland.|Laboratory for Surface Science and Technology, Department of Materials, ETH Zürich , 8093 Zurich, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Dübner
authors.affiliation: Laboratory for Micro- and Nanotechnology, Paul Scherrer Institute , 5232 Villigen PSI, Switzerland.
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Cadarso
authors.affiliation: Department of Chemistry, Bogazici University , 34342 Bebek, Istanbul, Turkey.
authors.forename: Tugce N
authors.initials: TN
authors.lastname: Gevrek
authors.affiliation: Department of Chemistry, Bogazici University , 34342 Bebek, Istanbul, Turkey.
authors.forename: Amitav
authors.identifier: http://orcid.org/0000-0001-5122-8329
authors.initials: A
authors.lastname: Sanyal
authors.affiliation: Laboratory for Surface Science and Technology, Department of Materials, ETH Zürich , 8093 Zurich, Switzerland.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Spencer
authors.affiliation: Laboratory for Micro- and Nanotechnology, Paul Scherrer Institute , 5232 Villigen PSI, Switzerland.
authors.forename: Celestino
authors.identifier: http://orcid.org/0000-0001-7577-5781
authors.initials: C
authors.lastname: Padeste
mesh_terms: D008027:Light; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers
keywords: lab-on-a-chip devices; light-responsive polymer brushes; orthogonal postpolymerization modification; smart materials; switchable enzyme activity