title: Discovering Protein-Coding Genes from the Environment: Time for the Eukaryotes?
abstract: Eukaryotic microorganisms from diverse environments encompass a large number of taxa, many of them still unknown to science. One strategy to mine these organisms for genes of biotechnological relevance is to use a pool of eukaryotic mRNA directly extracted from environmental samples. Recent reports demonstrate that the resulting metatranscriptomic cDNA libraries can be screened by expression in yeast for a wide range of genes and functions from many of the different eukaryotic taxa. In combination with novel emerging high-throughput technologies, we anticipate that this approach should contribute to exploring the functional diversity of the eukaryotic microbiota.
authors.affiliation: Ecologie Microbienne, UMR CNRS, UMR INRA, Université Claude Bernard Lyon 1, Université de Lyon, F-69622 Villeurbanne, France; Dipartimento di Scienze de la Vita e Biologia dei Sistemi, Università degli Studi di Torino, Torino, Italy. Electronic address: roland.marmeisse@univ-lyon1.fr.
authors.forename: Roland
authors.initials: R
authors.lastname: Marmeisse
authors.affiliation: Department of Bio- and Environmental Sciences, International Institute Zittau, Technische Universität Dresden, Markt 23, 02763 Zittau, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Kellner
authors.affiliation: Ecologie Microbienne, UMR CNRS, UMR INRA, Université Claude Bernard Lyon 1, Université de Lyon, F-69622 Villeurbanne, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Fraissinet-Tachet
authors.affiliation: Ecologie Microbienne, UMR CNRS, UMR INRA, Université Claude Bernard Lyon 1, Université de Lyon, F-69622 Villeurbanne, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Luis
mesh_terms: D056890:Eukaryota / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D064307:Microbiota / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger