title: Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells.
abstract: BACKGROUND
  The animal models of endometriosis could be a valuable alternative tool for clarifying the etiology of endometriosis.
  
  
  OBJECTIVE
  In this study two endometriosis models at the morphological and molecular levels was evaluated and compared.
  
  
  METHODS
  The human endometrial tissues were cut into small fragments then they were randomly considered for transplantation into γ irradiated mice as model A; or they were isolated and cultured up to fourth passages. 2×106cultured stromal cells were transplanted into γ irradiated mice subcutaneously as model B. twenty days later the ectopic tissues in both models were studied morphologically by Periodic acid-Schiff and hematoxylin and eosin staining. The expression of osteopontin (OPN) and matrix metalloproteinase 2 (MMP2) genes were also assessed using real time RT-PCR. 17-β estradiol levels of mice sera were compared before and after transplantation.
  
  
  RESULTS
  The endometrial like glands and stromal cells were formed in the implanted subcutaneous tissue of both endometriosis models. The gland sections per cubic millimeter, the expression of OPN and MMP2 genes and the level of 17-β estradiol were higher in model B than model A (p=0.03).
  
  
  CONCLUSIONS
  Our observation demonstrated that endometrial mesenchymal stromal cells showed more efficiency to establish endometriosis model than human endometrial tissue fragments.
authors.affiliation: Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Jafarabadi
authors.affiliation: Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mojdeh
authors.initials: M
authors.lastname: Salehnia
authors.affiliation: Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Rana
authors.initials: R
authors.lastname: Sadafi
keywords: Endometriosis; Matrix metalloproteinase 2; Osteopontin; Stromal cells