title: Motion characterization scheme to minimize motion artifacts in intravital microscopy.
abstract: Respiratory- and cardiac-induced motion artifacts pose a major challenge for in vivo optical imaging, limiting the temporal and spatial imaging resolution in fluorescence laser scanning microscopy. Here, we present an imaging platform developed for in vivo characterization of physiologically induced axial motion. The motion characterization system can be straightforwardly implemented on any conventional laser scanning microscope and can be used to evaluate the effectiveness of different motion stabilization schemes. This method is particularly useful to improve the design of novel tissue stabilizers and to facilitate stabilizer positioning in real time, therefore facilitating optimal tissue immobilization and minimizing motion induced artifacts.
authors.affiliation: Hanyang University, School of Electrical Engineering, Ansan, Republic of Korea.
authors.forename: Sungon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Richard B. Simches Research Center, Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Courties
authors.affiliation: Richard B. Simches Research Center, Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States.
authors.forename: Matthias
authors.initials: M
authors.lastname: Nahrendorf
authors.affiliation: Richard B. Simches Research Center, Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: Richard B. Simches Research Center, Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States.
authors.forename: Claudio
authors.initials: C
authors.lastname: Vinegoni
mesh_terms: D016477:Artifacts; D006321:Heart / Q000000981:diagnostic imaging; D006801:Humans; D000069416:Intravital Microscopy / Q000592:standards*; D018613:Microscopy, Confocal / Q000379:methods*; D009038:Motion; D012137:Respiratory System / Q000000981:diagnostic imaging