title: Development of Liver Decellularized Extracellular Matrix Bioink for Three-Dimensional Cell Printing-Based Liver Tissue Engineering.
abstract: The liver is an important organ and plays major roles in the human body. Because of the lack of liver donors after liver failure and drug-induced liver injury, much research has focused on developing liver alternatives and liver in vitro models for transplantation and drug screening. Although numerous studies have been conducted, these systems cannot faithfully mimic the complexity of the liver. Recently, three-dimensional (3D) cell printing technology has emerged as one of a number of innovative technologies that may help to overcome this limitation. However, a great deal of work in developing biomaterials optimized for 3D cell printing-based liver tissue engineering remains. Therefore, in this work, we developed a liver decellularized extracellular matrix (dECM) bioink for 3D cell printing applications and evaluated its characteristics. The liver dECM bioink retained the major ECM components of the liver while cellular components were effectively removed and further exhibited suitable and adjustable properties for 3D cell printing. We further studied printing parameters with the liver dECM bioink to verify the versatility and fidelity of the printing process. Stem cell differentiation and HepG2 cell functions in the liver dECM bioink in comparison to those of commercial collagen bioink were also evaluated, and the liver dECM bioink was found to induce stem cell differentiation and enhance HepG2 cell function. Consequently, the results demonstrate that the proposed liver dECM bioink is a promising bioink candidate for 3D cell printing-based liver tissue engineering.
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) , San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Hyungseok
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH) , 77 Cheongam ro, Nam-gu, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Wonil
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) , San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Hyeonji
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) , San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Dong-Heon
authors.initials: DH
authors.lastname: Ha
authors.affiliation: Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH) , San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Jinah
authors.initials: J
authors.lastname: Jang
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) , San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Byoung Soo
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH) , San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Dong-Woo
authors.identifier: 0000-0003-0630-5772
authors.initials: DW
authors.lastname: Cho
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D062028:Bioprinting / Q000379:methods*; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D066330:Printing, Three-Dimensional; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003094:Collagen