title: A real-time RT-qPCR assay for the detection of Citrus tatter leaf virus.
abstract: Citrus tatter leaf virus (CLTV) is globally distributed wherever citrus is grown, and, given the extensive use of CTLV sensitive rootstock, has the potential to be a significant threat to the citrus industry. In order to facilitate fast and reliable detection of this virus, we have developed a CTLV-specific real-time RT-qPCR assay. The optimized assay was found to be more reliable and sensitive compared to ELISA and end-point RT-PCR, detecting CTLV in up to 70% more plants. The real-time RT-qPCR is also specific, as it did not cross-react with the closely related Apple stem grooving virus or with the host itself; robust, being able to detect CTLV in young and mature host tissue types; and rapid.
authors.affiliation: Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA. Electronic address: sjcowell@ufl.edu.
authors.forename: S J
authors.initials: SJ
authors.lastname: Cowell
authors.affiliation: Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Harper
authors.affiliation: Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
authors.forename: W O
authors.initials: WO
authors.lastname: Dawson
mesh_terms: D002957:Citrus / Q000821:virology; D052677:Flexiviridae / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
keywords: Citrus; Citrus tatter leaf virus; Real-time RT-qPCR; TaqMan