title: Time-stretch microscopy on a DVD for high-throughput imaging cell-based assay.
abstract: Cell-based assay based on time-stretch imaging is recognized to be well-suited for high-throughput phenotypic screening. However, this ultrafast imaging technique has primarily been limited to suspension-cell assay, leaving a wide range of solid-substrate assay formats uncharted. Moreover, time-stretch imaging is generally restricted to intrinsic biophysical phenotyping, but lacks the biomolecular signatures of the cells. To address these challenges, we develop a spinning time-stretch imaging assay platform based on the functionalized digital versatile disc (DVD). We demonstrate that adherent cell culture and biochemically-specific cell-capture can now be assayed with time-stretch microscopy, thanks to the high-speed DVD spinning motion that naturally enables on-the-fly cellular imaging at an ultrafast line-scan rate of >10MHz. As scanning the whole DVD at such a high speed enables ultra-large field-of-view imaging, it could be favorable for scaling both the assay throughput and content as demanded in many applications, e.g. drug discovery, and rare cancer cell screening.
authors.affiliation: Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Anson H L
authors.initials: AH
authors.lastname: Tang
authors.affiliation: Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: P
authors.initials: P
authors.lastname: Yeung
authors.affiliation: Department of Paediatrics & Adolescent Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Godfrey C F
authors.initials: GC
authors.lastname: Chan
authors.affiliation: Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Barbara P
authors.initials: BP
authors.lastname: Chan
authors.affiliation: Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Kenneth K Y
authors.initials: KK
authors.lastname: Wong
authors.affiliation: Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Kevin K
authors.initials: KK
authors.lastname: Tsia
keywords: (110.0110) Imaging systems; (170.0180) Microscopy; (170.3880) Medical and biological imaging; (170.7160) Ultrafast technology