title: Introduction of Thinopyrum intermedium ssp. trichophorum chromosomes to wheat by trigeneric hybridization involving Triticum, Secale and Thinopyrum genera.
abstract: Fluorescence in situ hybridization and molecular markers have confirmed that several chromosomes from Thinopyrum intermedium ssp. trichophorum have been added to a wheat background, which originated from a cross between a wheat- Thinopyrum partial amphiploid and triticale. The lines displayed blue grains and resistance to wheat stripe rust. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. With the aim to transfer novel genetic variation from Th. intermedium species for sustainable wheat breeding, a new trigeneric hybrid was produced by crossing an octoploid wheat-Th. intermedium ssp. trichophorum partial amphiploid with hexaploid triticale. Fluorescence in situ hybridization (FISH) revealed that Thinopyrum chromosomes were transmitted preferably and the number of rye chromosomes tended to decrease gradually in the selfed derivatives of the trigeneric hybrids. Four stable wheat-Th. intermedium chromosome substitution, addition and translocation lines were selected, and a 2JSaddition line, two substitution lines of 4JS(4B) and 4J(4B), and a small 4J.4B translocation line were identified by FISH and molecular markers. It was revealed that the gene(s) responsible for blue grains may located on the FL0.60-1.00 of long arm of Th. intermedium-derived 4J chromosome. Disease resistance screenings indicated that chromosomes 4JSand 2JSappear to enhance the resistance to stripe rust in the adult plant stage. The new germplasm with Th. intermedium introgression shows promise for utilization of Thinopyrum chromosome segments in future wheat improvement.
authors.affiliation: Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lang
authors.affiliation: Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
authors.forename: Hongjin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
authors.forename: Guangrong
authors.initials: G
authors.lastname: Li
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
authors.forename: Ennian
authors.initials: E
authors.lastname: Yang
authors.affiliation: Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China. yangzujun@uestc.edu.cn.
authors.forename: Zujun
authors.initials: Z
authors.lastname: Yang
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000235:genetics*; D012434:Secale / Q000235:genetics*; D014908:Triticum / Q000235:genetics*