title: [Detection and analysis of plasma VWF levels in healthy Chinese by automatic standardized assay].
abstract: Objective:To explore the normal range of plasma VWF levels of healthy Chinese and to analyze the influencing factors to VWF level.Methods:To detect the levels of von Willebrand factor antigen (VWF∶Ag) , von Willebrand factor ristocetin cofactor activity (VWF∶Rco) , von Willebrand factor collagen binding activity (VWF∶CB) , and the factor Ⅷ coagulation activity (FⅧ∶C) by using fully automatic and standardized testing instruments and matching reagent in 70 healthy Chinese. The effects of age, ABO blood type, gender and region were also analyzed. Meanwhile, 8 standard plasma samples (2 normal subjects, 6 cases of type 2 VWD) confirmed by NIBSC were tested for VWF values.Results:① In 70 cases of healthy Chinese, the mean value of plasma VWF∶Ag, VWF∶Rco and VWF∶CB were (95.4±44.9) %, (105.9±35.4) % and (89.8±28.4) %, respectively; the ratio of VWF∶Rco/VWF∶Ag and VWF∶CB/VWF∶Ag was 1.18±0.25 and 1.03±0.29, respectively. ②There was no statistical significance in plasma VWF values between the age ≥30 years and <30 years group (P>0.05) . ③The VWF∶Rco, VWF∶CB of type O blood group were lower than that of non-O group (t=2.074,P=0.042;t=3.949,P=0.001) , but there was no statistical significance in VWF∶Ag, VWF∶Rco/VWF∶Ag, VWF∶CB/VWF∶Ag between the two groups (P>0.05) . ④There was no significant difference in VWF values between male and female groups (P>0.05) . ⑤The VWF∶Ag, VWF∶CB of the northern population (North area of Huaihe River) group were higher than that of southern population (Suzhou area) group (t=4.525,P=0.001;t=3.214,P=0.002) , but VWF∶Rco/VWF∶Ag, VWF∶CB/VWF∶Ag were lower than that of southern population group (t=6.373,P=0.001;t=2.902,P=0.005) , and there was no significant difference in VWF∶Rco between the two groups (t=1.598,P=0.115) . ⑥The VWF values of 8 standard plasma samples were in accordance with the known diagnosis.Conclusions:A more integrate plasma VWF levels of healthy Chinese people were obtained for the first time by using fully automatic and standardized testing instruments. It was also found that ABO blood group and region had a significant impact on the level of VWF, while the age and gender had no significant effect.
authors.affiliation: Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, China.
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Yu
authors.forename: X H
authors.initials: XH
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Bai
authors.forename: L J
authors.initials: LJ
authors.lastname: Cao
authors.forename: J
authors.initials: J
authors.lastname: Su
authors.forename: P P
authors.initials: PP
authors.lastname: Sha
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: C G
authors.initials: CG
authors.lastname: Ruan
mesh_terms: D000017:ABO Blood-Group System; D044466:Asian People; D001780:Blood Coagulation Tests; D001788:Blood Grouping and Crossmatching; D005260:Female; D006801:Humans; D008297:Male; D056728:von Willebrand Disease, Type 2; D014841:von Willebrand Factor
chemical_list: D000017:ABO Blood-Group System; D014841:von Willebrand Factor
keywords: ABO blood-group system; Reference values; Sex factors; von Willebrand diseases; von Willebrand factor