title: Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle.
abstract: MicroRNAs (miRNAs) are important post-transcriptional regulators involved in animal adipogenesis, however, their roles in bovine fat deposition remain poorly understood. In the present study, we conducted a comparative RNA sequencing to identify the key miRNAs involved in beef lipid accumulation by comparing the backfat small RNA samples between Wagyu (high intramuscular fat) and Holstein (moderate intramuscular fat) cattle. Fifteen miRNAs such as bta-miR-142-3p, bta-miR-379, bta-miR-196a, bta-miR-196b, bta-miR-30f and bta-miR-2887 were identified to have a higher expression level in Wagyu cattle compared with Holstein, whereas bta-miR-320a, bta-miR-874 and bta-miR-1247-3p had a lower expression level in Wagyu. Furthermore, a total of 1345 potential target genes of differentially expressed miRNAs were predicted using bioinformatics tools, in which PPARα and RXRα were known to play a critical role in adipocyte differentiation and lipid metabolism. In conclusion, the present study constructed a high-throughput RNA sequencing screen and successfully identified miRNAs such as bta-miR-874, bta-miR-320a and bta-miR-196b which may affect beef fat deposition. The present findings may provide a theoretical foundation for the utilization of beef cattle germplasm resources.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Wanlong
authors.initials: W
authors.lastname: Huang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Miao
mesh_terms: D000818:Animals; D002417:Cattle; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000378:metabolism*; D060066:Protein Interaction Maps; D015398:Signal Transduction; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D035683:MicroRNAs