title: [Chronic stress model in New Zealand white rabbit with hyperlipidemia].
abstract: Objective:To establish and evaluate chronic stress model in New Zealand white rabbit with hyperlipidemia.Methods:A total of 45 clearing grade male New Zealand white rabbits were divided into four groups with random number table method: control (CON), normal diet combined with chronic stress for 8 weeks (CON+ CS), high fat diet (HFD) and high fat diet for 4 weeks combined with chronic stress for 8 weeks (HFD+ CS). Both social stress and physical stress methods were adopted.One-way ANOVA was used for comparison among groups.Results:(1) Chronic stress model assessments: ①body weight, the weight gain of stress groups was significantly reduced; ②behavioral assessment, rabbits exposed to stress in CON+ CS and HFD+ CS group [54%±7%, 55%±5%] exhibited more inactivity behavior than CON and HFD group [27%±5.28%, 34%±6%,P<0.01,P<0.05]; ③serum indexes: after stress regime for 4 weeks, cortisol of HFD+ CS was higher than HFD group [(60±5) ng/ml vs (38±4) ng/ml,P=0.001]. After 8 weeks, the serum levels of hs-CRP and IL-6 also elevated. (2) The effect of hyperlipidemia on chronic stress: compared with CON+ CS, HFD+ CS group showed more inactivity behavior and rising levels of cortisol, hs-CRP and IL-6. (3) Blood lipids: chronic stress induced raised serum total cholesterol.Conclusions:(1)Chronic stress model in rabbit with hyperlipidemia could be successfully established with 4-week high lipid feed followed by social stress combined with physical stress for 8 weeks.(2) Hyperlipidemia and chronic stress influences each other.
authors.affiliation: The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, China.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: L Y
authors.initials: LY
authors.lastname: Xiao
authors.forename: X T
authors.initials: XT
authors.lastname: Deng
authors.forename: T
authors.initials: T
authors.lastname: Gong
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D006949:Hyperlipidemias; D015850:Interleukin-6; D008055:Lipids; D008297:Male; D011817:Rabbits; D013312:Stress, Physiological
chemical_list: D015850:Interleukin-6; D008055:Lipids
keywords: Behavioral research; Chronic stress; Hyperlipidemia; New Zealand white rabbit; Social stress