title: Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship.
abstract: This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) A. Löve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive DNA probes proved suitable for the identification of individual chromosomes in the diploid JJ, StSt and PP genomes. Of the seven microsatellite markers tested only the (GAA)n trinucleotide sequence was appropriate for use as a single chromosome marker for the P. spicata AS chromosome. Based on COS marker analysis, the phylogenetic relationship between diploid wheatgrasses and the hexaploid bread wheat genomes was established. These findings confirmed that the J and E genomes are in neighbouring clusters.
authors.affiliation: Department of Plant Genetic Resources, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
authors.forename: Gabriella
authors.identifier: http://orcid.org/0000-0002-3149-9786
authors.initials: G
authors.lastname: Linc
authors.affiliation: Department of Plant Genetic Resources, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Gaál
authors.affiliation: Department of Plant Genetic Resources, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Molnár
authors.affiliation: Department of Plant Genetic Resources, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
authors.forename: Diana
authors.initials: D
authors.lastname: Icsó
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Badaeva
authors.affiliation: Department of Plant Genetic Resources, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
authors.forename: Márta
authors.initials: M
authors.lastname: Molnár-Láng
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D004171:Diploidy; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D006109:Poaceae / Q000145:classification / Q000235:genetics*