title: New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans.
abstract: Mitochondria are highly dynamic organelles that constantly fuse and divide. This process is essential as several neurodegenerative diseases have been associated with defects in mitochondrial fusion or fission. Several tools have been developed over the years to visualize mitochondria in organisms such as Caenorhabditis elegans. Combining these tools with the powerful genetics of C. elegans has led to the discovery of new regulators of mitochondrial morphology. In this chapter, we present additional tools to further characterize mitochondrial morphology as well as regulators of mitochondrial morphology. Specifically, we introduce a photoactivatable mitoGFP (PAmitoGFP) that allows to investigate the connectivity of complex mitochondrial networks. In addition, we describe an immunostaining protocol that enables localization studies of these newly identified regulators of mitochondrial morphology.
authors.affiliation: LMU Biocenter, Department Biology II, Ludwig-Maximilians-University Munich, Grosshadernerstr. 2, Planegg-Martinsried, Munich, 82152, Germany.|Department of Genetics, Geisel School of Medicine at Dartmouth, 7400 Remsen, Hanover, NH, 03755, USA.|National Institute of Child Health and Human Development, NIH, 18 Center Drive, Bethesda, 20892, MD, USA.
authors.forename: Saroj G
authors.initials: SG
authors.lastname: Regmi
authors.affiliation: LMU Biocenter, Department Biology II, Ludwig-Maximilians-University Munich, Grosshadernerstr. 2, Planegg-Martinsried, Munich, 82152, Germany. rolland@bio.lmu.de.
authors.forename: Stéphane G
authors.initials: SG
authors.lastname: Rolland
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D017930:Genes, Reporter; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure*; D008931:Mitochondria, Muscle / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D063154:Mitochondrial Dynamics; D057054:Molecular Imaging / Q000379:methods*
keywords: Caenorhabditis elegans; Immunostaining; Mitochondrial dynamics; Mitochondrial morphology; PAmitoGFP