title: Label-free detection of sex determining region Y (SRY) via capacitive biosensor.
abstract: In this work, we present for the first time, the use of a simple fractal capacitive biosensor for the quantification and detection of sex-determining region Y (SRY) genes. This section of genetic code, which is found on the Y chromosome, finds importance for study as it causes fetuses to develop characteristics of male sex-like gonads when a mutation occurs. It is also an important genetic code in men, and disorders involving the SRY gene can cause infertility and sexual malfunction that lead to a variety of gene mutational disorders. We have therefore designed silicon-based, label-free fractal capacitive biosensors to quantify various proteins and genes. We take advantage of a good dielectric material, Parylene C for enhancing the performance of the sensors. We have integrated these sensors with a simple microchannel for easy handling of fluids on the detection area. The read-out value of an Agilent LCR meter used to measure capacitance of the sensor at a frequency of 1 MHz determined gene specificity and gene quantification. These data revealed that the capacitance measurement of the capacitive biosensor for the SRY gene depended on both the target and the concentration of DNA. The experimental outcomes in the present study can be used to detect DNA and its variations in crucial fields that have a great impact on our daily lives, such as clinical and veterinary diagnostics, industrial and environmental testing and forensic sciences.
authors.forename: Shilpa
authors.initials: S
authors.lastname: Sivashankar
authors.forename: Christos
authors.initials: C
authors.lastname: Sapsanis
authors.forename: Sumeyra
authors.initials: S
authors.lastname: Agambayev
authors.forename: Ulrich
authors.initials: U
authors.lastname: Buttner
authors.forename: Khaled Nabil
authors.initials: KN
authors.lastname: Salama
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D041322:Chromosomes, Human, Y; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D018625:Microscopy, Atomic Force; D009693:Nucleic Acid Hybridization; D011108:Polymers / Q000737:chemistry; D051876:Sex-Determining Region Y Protein / Q000032:analysis / Q000235:genetics; D012825:Silicon / Q000737:chemistry; D014992:Xylenes / Q000737:chemistry
chemical_list: D061925:Immobilized Nucleic Acids; D011108:Polymers; D051876:Sex-Determining Region Y Protein; D014992:Xylenes; C011055:parylene; D004247:DNA; D012825:Silicon