title: DNA methylation modulates H19 and IGF2 expression in porcine female eye.
abstract: The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye.
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Emergency, First Hospital, Jilin University, Changchun, Jilin, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Liu
authors.affiliation: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangdong, Guangzhou, China.
authors.forename: Cuie
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Emergency, First Hospital, Jilin University, Changchun, Jilin, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lin
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Dianfeng
authors.initials: D
authors.lastname: Liu