title: Evidence of natural co-infection with PCV2b subtypes in vivo.
abstract: Porcine circovirus type 2 (PCV2) is the causative pathogen of porcine circovirus-associated diseases (PCVAD). This virus evolves mostly through point mutations and genome recombination between different PCV2 genotypes (e.g. PCV2a and PCV2b), as has been confirmed in swine herds. In the current work, the complete PCV2 genome sequences of 69 clones derived from various tissues (lymph node, spleen and lung,) of an infected individual, were subjected to phylogenetic and alignment analyses. The results not only demonstrate co-infection with distinct PCV2b subtypes (e.g. 1B and 1C) in the same animal, but also highlight another mechanism of evolution - diverse point mutations acquired during immune evasion by this virus.
authors.affiliation: Laboratory of Animal Models and Functional Genomics (LAMFG), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Laboratory of Functional Proteomics (LFP), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Laboratory of Functional Proteomics (LFP), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Laboratory of Functional Proteomics (LFP), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xie
authors.affiliation: Laboratory of Animal Models and Functional Genomics (LAMFG), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Tanbin
authors.initials: T
authors.lastname: Liu
authors.affiliation: Laboratory of Animal Models and Functional Genomics (LAMFG), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Laboratory of Functional Proteomics (LFP), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Naidong
authors.initials: N
authors.lastname: Wang
authors.affiliation: Laboratory of Functional Proteomics (LFP), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Zhibang
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Laboratory of Animal Models and Functional Genomics (LAMFG), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Lei
authors.affiliation: Laboratory of Functional Proteomics (LFP), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Laboratory of Animal Models and Functional Genomics (LAMFG), Research Center of Reverse Vaccinology (RCRV), College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China. bingaiwang@hunau.edu.cn.
authors.forename: Aibing
authors.initials: A
authors.lastname: Wang
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060085:Coinfection / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000821:virology*