title: An Investigation on the Expression Level of Interleukin 10 (IL-10) in the Healthy and Mastitic Holstein Cows and the Bioinformatics Analysis of Nucleosome Profile.
abstract: Cytokines are immune regulators that play an essential role in regulating immune response against various infections. The present study focused on the possible association between the expression level of Interleukin 10 (IL-10) in blood and milk samples of 25 healthy and 25 mastitic cows in Fars province, Iran, using a quantitative real-time PCR assay. The experimental groups were categorized according to the number of calvings. The expression level of IL-10 was significantly higher in the blood and milk samples of mastitic cows compared to the healthy ones. Concomitant to increasing the number of calving, a numerical elevation in the expression of IL-10 in blood was observed (P < 0.05). The bioinformatics analysis of IL-10 gene revealed the promoter, exon-intron regions, and nucleosome profile. The nucleosome occupancy site was finally predicted using NUPOP software. Our result indicated that the promoter was not exactly placed in the nucleosome region, which was finally aimed to predict the position and expression of IL-10 gene in the mastitic cows.
authors.affiliation: a Department of Animal Breeding, School of Agriculture , Yasouj University , Yasouj , Iran.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Varzandian
authors.affiliation: a Department of Animal Breeding, School of Agriculture , Yasouj University , Yasouj , Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ghaderi-Zefrehei
authors.affiliation: b Department of Food Hygiene and Public Health, School of Veterinary Medicine , Shiraz University , Shiraz , Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Hosseinzadeh
authors.affiliation: b Department of Food Hygiene and Public Health, School of Veterinary Medicine , Shiraz University , Shiraz , Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Sayyadi
authors.affiliation: b Department of Food Hygiene and Public Health, School of Veterinary Medicine , Shiraz University , Shiraz , Iran.
authors.forename: Vahideh
authors.initials: V
authors.lastname: Taghadosi
authors.affiliation: c Department of Clinical Sciences, School of Veterinary Medicine, Kazerun Branch , Islamic Azad University , Kazerun , Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Varzandian
mesh_terms: D000818:Animals; D002417:Cattle; D019295:Computational Biology; D005260:Female; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D007492:Iran; D008414:Mastitis, Bovine / Q000235:genetics / Q000378:metabolism; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016753:Interleukin-10
keywords: Bioinformatics analysis; Interloukin-10; clinical mastitis; gene Expression; real-time PCR