title: Studying the Immune Synapse in HIV-1 Infection.
abstract: T cells are the main cellular targets of the human immunodeficiency virus 1 (HIV-1). HIV-1 infection induces pleiotropic effects on the infected T cell that modify the T cell capacity to respond to antigen and facilitates virus replication. HIV-1 infection subverts the formation and function of the immunological synapse altering both actin cytoskeleton remodeling and intracellular vesicle traffic. We describe here our methods to unveil how HIV-1 and in particular its protein Nef modify vesicle traffic to the immunological synapse, perturbing the synapse activation capacity.
authors.affiliation: Institut Pasteur, Department of Immunology, Lymphocyte Cell Biology Unit, Paris, France.|INSERM U-1221, Paris, France.
authors.forename: Iratxe
authors.initials: I
authors.lastname: Del Río-Iñiguez
authors.affiliation: Institut Pasteur, Department of Immunology, Lymphocyte Cell Biology Unit, Paris, France.|INSERM U-1221, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bouchet
authors.affiliation: Institut Pasteur, Department of Immunology, Lymphocyte Cell Biology Unit, Paris, France. andres.alcover@pasteur.fr.|INSERM U-1221, Paris, France. andres.alcover@pasteur.fr.
authors.forename: Andrés
authors.initials: A
authors.lastname: Alcover
mesh_terms: D015488:HIV Antigens / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D054992:Immunological Synapses / Q000276:immunology* / Q000473:pathology; D019169:Jurkat Cells; D014779:Virus Replication / Q000276:immunology*; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D015488:HIV Antigens; D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1
keywords: Cytoskeleton; Endosomes; HIV-1; Immunological synapse; Nef; T cell activation; TCR signaling; Vesicle traffic