title: Functional interaction of PkcA and PldB regulate aggregation and development in Dictyostelium discoideum.
abstract: Multicellular development in Dictyostelium discoideum involves tightly regulated signaling events controlling the entry into development, initiation of aggregation and chemotaxis, and cellular differentiation. Here we show that PkcA, a Dictyostelium discoideum Protein Kinase C-orthologue, is involved in quorum sensing and the initiation of development, as well as cAMP sensing during chemotaxis. Additionally, by epistasis analysis we provide evidence that PkcA and PldB (a Phospholipase D-orthologue) functionally interact to regulate aggregation, differentiation, and cell-cell adhesion during development. Finally, we show that PkcA acts as a positive regulator of intracellular PLD-activity during development. Taken together, our results suggest that PkcA act through PldB, by regulating PLD-activity, in order to control events during development.
authors.affiliation: Department of Biological Sciences, Hunter College and The Graduate Center, The City University of New York, New York, NY, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology-Madras, Chennai 600036, India.
authors.forename: Wasima
authors.initials: W
authors.lastname: Mohamed
authors.affiliation: Department of Biological Sciences, Hunter College and The Graduate Center, The City University of New York, New York, NY, USA.
authors.forename: Annelie
authors.initials: A
authors.lastname: Aguessy
authors.affiliation: Department of Biological Sciences, Hunter College and The Graduate Center, The City University of New York, New York, NY, USA.
authors.forename: Ella
authors.initials: E
authors.lastname: Dyett
authors.affiliation: Department of Biological Sciences, Hunter College and The Graduate Center, The City University of New York, New York, NY, USA.
authors.forename: Shriraj
authors.initials: S
authors.lastname: Shah
authors.affiliation: Department of Biological Sciences, Hunter College and The Graduate Center, The City University of New York, New York, NY, USA.
authors.forename: Mohammedasad
authors.initials: M
authors.lastname: Khan
authors.affiliation: Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology-Madras, Chennai 600036, India.
authors.forename: Ramamurthy
authors.initials: R
authors.lastname: Baskar
authors.affiliation: Department of Biological Sciences, Hunter College and The Graduate Center, The City University of New York, New York, NY, USA. Electronic address: brazill@genectr.hunter.cuny.edu.
authors.forename: Derrick
authors.initials: D
authors.lastname: Brazill
mesh_terms: D000440:Butanols / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation; D016764:Cell Polarity / Q000187:drug effects; D002633:Chemotaxis; D000242:Cyclic AMP / Q000378:metabolism; D004023:Dictyostelium / Q000254:growth & development / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000494:pharmacology; D010739:Phospholipase D / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D053038:Quorum Sensing / Q000502:physiology; D015398:Signal Transduction
chemical_list: D000440:Butanols; D015815:Cell Adhesion Molecules; D010712:Phosphatidic Acids; D015800:Protozoan Proteins; C052291:cell cohesion molecule, Dictyostelium; D000242:Cyclic AMP; D051571:Protein Kinase C-alpha; D010739:Phospholipase D; C106801:phospholipase D1
keywords: Actin cytoskeleton; Development; Dictyostelium; PKC; PLD; Quorum sensing