title: Down-regulation of miR-655-3p predicts worse clinical outcome in patients suffering from hepatocellular carcinoma.
abstract: MiR-655-3p has been reported to play important roles in tumor initiation, development, and metastasis in several cancers. This study aimed to assess the potential role of miR-655-3p in the pathogenesis of hepatocellular carcinoma (HCC).
  
  
  
  The expression levels of miR-655-3p in HCC tissues were detected by qPCR. The relationship between clinicopathologic characteristics and miR-655-3p was analyzed by chi-square test. Kaplan-Meier curves and multivariate Cox proportional models were used to study the impact on clinical outcome.
  
  
  
  miR-655-3p was significantly down- regulated in HCC tissues compared to normal adjacent liver tissues (p < 0.01). Low miR-655-3p expression was observed to be closely correlated with positive microvascular invasion, advanced tumor stage and lymph node metastasis (p < 0.05, respectively). The results of Kaplan-Meier analysis showed that patients with high miR-655-3p expression lived shorter than those with low miR-655-3p expression (Log-rank test, p = 0.0002). Multivariate analysis revealed that miR-655-3p was an independent risk factor for HCC (HR=1.533, 95% CI: 0.988-3.891; p = 0.002).
  
  
  
  Our data showed that low expression of miR-655-3p was associated with significant characteristics of patients with HCC, and it could function as a potential unfavorable prognostic biomarker.
authors.affiliation: Hospital & Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China. zhaoxq8014@163.com.
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Zhao
authors.forename: B
authors.initials: B
authors.lastname: Liang
authors.forename: K
authors.initials: K
authors.lastname: Jiang
authors.forename: H-Y
authors.initials: HY
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C585264:MIRN655 microRNA, human; D035683:MicroRNAs