title: Brain proteomic modifications associated to protective effect of grape extract in a murine model of obesity.
abstract: In recent years, the obesity epidemic has developed into a major health crisis worldwide. With current treatments limited to expensive, high-risk surgery and minimally efficacious pharmacotherapy, new therapeutic options are urgently needed to fight against this alarming trend. Though brain dysfunction has been studied linked to high fat diet (HFD) and grape seed and skin extract (GSSE) correction, the proteomic modifications linking the two effects on brain lipotoxicity are not well understood. To this end rats were exposed for 8 weeks to HFD treatment, to GSSE (500mg/kg BW) and to binary mixture of HFD and GSSE to gain insight into the potential pathways altered with metabolic disease and the protection afforded by GSSE. Significant modifications of brain proteins were detected using mass spectrometry-based differential proteomics. These proteins were mainly related to oxidative stress, glycolysis and calcium signaling. Additionally, proteins involved in the cytoskeleton were also affected by HFD treatment. Interestingly, whether up- or down regulated protein abundances, GSSE corrected most of the disturbances of HFD treatment. These findings provide impetus for future therapeutic investigation on GSSE against other metabolic disorders.
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie; Laboratoire Polymères, Biopolymères, Membranes, UMR6270 CNRS, IRIB, Normandie Université, Mont Saint Aignan, France; Université Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Neurophysiologie et Pathologies Fonctionnelles, Tunis, Tunisie.
authors.forename: Selima
authors.initials: S
authors.lastname: Smine
authors.affiliation: Laboratoire Polymères, Biopolymères, Membranes, UMR6270 CNRS, IRIB, Normandie Université, Mont Saint Aignan, France; Plateforme Protéomique PISSARO, Institut de Recherche et d'Innovation Biomédicale, Normandie Université, Mont Saint Aignan, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Obry
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie.
authors.forename: Safwen
authors.initials: S
authors.lastname: Kadri
authors.affiliation: Laboratoire Polymères, Biopolymères, Membranes, UMR6270 CNRS, IRIB, Normandie Université, Mont Saint Aignan, France; Plateforme Protéomique PISSARO, Institut de Recherche et d'Innovation Biomédicale, Normandie Université, Mont Saint Aignan, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Hardouin
authors.affiliation: Laboratoire Physiopathologie et Biothérapie des Maladies Inflammatoires et Autoimmunes, UMR-S905, INSERM, IRIB, Normandie Université, Rouen, France.
authors.forename: Manuel
authors.initials: M
authors.lastname: Fréret
authors.affiliation: Université Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Neurophysiologie et Pathologies Fonctionnelles, Tunis, Tunisie.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Amri
authors.affiliation: Laboratoire Polymères, Biopolymères, Membranes, UMR6270 CNRS, IRIB, Normandie Université, Mont Saint Aignan, France; Plateforme Protéomique PISSARO, Institut de Recherche et d'Innovation Biomédicale, Normandie Université, Mont Saint Aignan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie.
authors.forename: Ferid
authors.initials: F
authors.lastname: Limam
authors.affiliation: Laboratoire Polymères, Biopolymères, Membranes, UMR6270 CNRS, IRIB, Normandie Université, Mont Saint Aignan, France; Plateforme Protéomique PISSARO, Institut de Recherche et d'Innovation Biomédicale, Normandie Université, Mont Saint Aignan, France. Electronic address: pascal.cosette@univ-rouen.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie.
authors.forename: Ezzedine
authors.initials: E
authors.lastname: Aouani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D059305:Diet, High-Fat; D005786:Gene Expression Regulation / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D056604:Grape Seed Extract / Q000008:administration & dosage*; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D040901:Proteomics; D051381:Rats
chemical_list: D000975:Antioxidants; D056604:Grape Seed Extract