title: A new azadirachta from the crude extracts of neem (Azadirachta Indica A. Juss) seeds.
abstract: One new compound, 2H, 3H-cyclopent[b] furo [2',3':4,5] naphtho [2,4-d] heptlactone-[3,7] furan-6aceticacid, 3-(acetyloxy)-8-(3-furyl)-2a, 4a, 4b, 4c,5,5a, 6, 6a, 8, 9,9a, 10a,10b-13 hydrogen-2a,5a,6a,5-tetramethyl-3-[[(2E)-2-methyl-1-oxo-2-butenyl]oxy]-methyl ester, named azadirachta R (1), along with 10 known ones, Azadirachta A, AZ-B, AZ-D, AZ-H, AZ-I, nimbin, deacetylnimbin, salannin, deacetylsalannin and azadiradione were isolated from the crude extracts of neem (Azadirachta indica A. Juss) seeds, which were determined by UV, IR, HR-ESI-MS and NMR data analyses. According to the in vitro antibacterial activity experimental results, this compound showed good antibacterial activity to two bacteria, the minimum inhibitory concentration and the minimum bactericidal concentration of compound 1 to two kinds of bacteria are 50 and 25 mg/L, respectively, which were determined by resazurin colour-micro-dilution method. The experimental results provide a theoretical basis for the comprehensive utilisation of azadirachtin compounds in the future.
authors.affiliation: a School of Plant Protection , Anhui Agricultural University , Hefei , China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Gao
authors.affiliation: b State Forestry Administration Key Open Laboratory , International Centre for Bamboo and Rattan , Beijing , China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.affiliation: b State Forestry Administration Key Open Laboratory , International Centre for Bamboo and Rattan , Beijing , China.
authors.forename: Hang
authors.initials: H
authors.lastname: Xun
authors.affiliation: b State Forestry Administration Key Open Laboratory , International Centre for Bamboo and Rattan , Beijing , China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yao
authors.affiliation: b State Forestry Administration Key Open Laboratory , International Centre for Bamboo and Rattan , Beijing , China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: b State Forestry Administration Key Open Laboratory , International Centre for Bamboo and Rattan , Beijing , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D031601:Azadirachta / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D000077222:Limonene; D036701:Limonins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D036701:Limonins; D010936:Plant Extracts; D014315:Triterpenes; C000624636:azadirachta R; C479321:azadiradione; C105836:salannin; D000077222:Limonene; C008621:nimbin; C010329:azadirachtin
keywords: Azadirachta R; in vitro antibacterial activity; resazurin colour-micro-dilution method