title: [Lipopolysaccharide of Porphyromonas gingivalis promotes the autophagy of human gingival fibroblasts].
abstract: Objective To investigate the impact of lipopolysaccharide of Porphyromonas gingivalis (Pg-LPS) on the autophagy of human gingival fibroblasts (HGFs). Methods Firstly, HGFs was stimulated with 10 μg/mL Pg-LPS for 12 hours or 24 hours. Rapamycin was used as a positive control. The expression of LC3B was detected by Western blotting and the distribution of autophagosomes was observed by indirect immunofluorescence staining. At the same time, mitochondrial ROS (mtROS) was labeled by MitoSOX Red. The levels of mtROS and mitochondrial autophagy were measured in HGFs after treated with Pg-LPS. Then, T-butyl-4 (BHA), N-acetylcysteine (NAC) and coenzyme Q10 (CoQ10) were used separately to block the ROS and the expression of LC3B in Pg-LPS-treated HGFs was tested by Western blotting. Results After treatment with Pg-LPS, the ratio of LC3BII/LC3BI and the number of autophagic cells significantly increased, and the increase in the 24-hour treatment group was the more obvious than that in the 12-hour treatment group. The mtROS production and mitochondrial autophagy were significantly promoted after Pg-LPS treatment. In addition, CoQ10 effectively reduced Pg-LPS-induced autophagy of HGFs. Conclusion Pg-LPS can induce the autophagy of HGFs by raising mtROS production, and autophagy is involved in the degradation of damaged mitochondria to maintain cellular homeostasis.
authors.affiliation: Department of Stomatology, Second People's Hospital of Hefei City, Hefei 230011, China. *Corresponding author, E-mail: liuyulorry@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Second People's Hospital of Hefei City, Hefei 230011, China.
authors.forename: Shujin
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Stomatology, Nanjing General Hospital of Nanjing Military Area Command, Nanjing 210002, China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Second People's Hospital of Hefei City, Hefei 230011, China.
authors.forename: Xiaoma
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Stomatology, Second People's Hospital of Hefei City, Hefei 230011, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001343:Autophagy; D016866:Bacteroidaceae Infections / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology*; D005347:Fibroblasts / Q000166:cytology* / Q000382:microbiology; D005881:Gingiva / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D014451:Ubiquinone; C024989:coenzyme Q10