title: Ultrafast Fluorescence Dynamics in Flurbiprofen-Amino Acid Dyads and in the Supramolecular Drug/Protein Complex.
abstract: The interaction dynamics between the drug flurbiprofen (FBP) and human serum albumin (HSA) has been investigated by time-resolved fluorescence spectroscopy, combining femtosecond fluorescence upconversion and picosecond time-correlated single photon counting. In order to obtain additional information on the drug/ protein interaction, several covalently linked model dyads, composed of FBP and tryptophan or tyrosine, were also studied. For all systems, the main feature was a remarkable dynamic FBP fluorescence quenching, more prominent in the dyads than in the protein complex. All systems also displayed a clear stereoselectivity depending on the (S)- or (R)-form of FBP, that was strongly influenced by the conformational arrangement of the investigated chromophores.
authors.affiliation: Departamento de Química/Instituto de Tecnología, Química UPV-CSIC, Universitat Politècnica de València Camino de Vera s/n, 46022 Valencia, Spain, School of Chemistry, University of East Anglia, Norwich Research Park NR4 7TJ, Norwich, United Kingdom;, Email: i.vaya-perez@uea.ac.uk.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Vayá
authors.affiliation: Departamento de Química/Instituto de Tecnología, Química UPV-CSIC, Universitat Politècnica de València Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: M Consuelo
authors.initials: MC
authors.lastname: Jiménez
authors.affiliation: Departamento de Química/Instituto de Tecnología, Química UPV-CSIC, Universitat Politècnica de València Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Miranda
authors.affiliation: LIDYL, Laboratoire Interactions, Dynamiques et Lasers CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191 Gif-sur-Yvette, France.
authors.forename: Aninda
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: LIDYL, Laboratoire Interactions, Dynamiques et Lasers CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191 Gif-sur-Yvette, France;, Email: thomas.gustavsson@cea.fr.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gustavsson
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D005453:Fluorescence; D005480:Flurbiprofen / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D012709:Serum Albumin / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D012709:Serum Albumin; D005480:Flurbiprofen