title: β-Catenin promotes cell proliferation, migration, and invasion but induces apoptosis in renal cell carcinoma.
abstract: β-Catenin (CTNNB1gene coding protein) is a component of the Wnt signaling pathway that has been shown to play an important role in the formation of certain cancers. Abnormal accumulation ofCTNNB1contributes to most cancers. This research studied the involvement of β-catenin in renal cell carcinoma (RCC) cell proliferation, apoptosis, migration, and invasion. Proliferation, cell cycle, and apoptosis were analyzed by using Cell Counting Kit-8 and by flow cytometry. Migration and invasion assays were measured by transwell analysis. Real-time polymerase chain reaction and Western blot analysis were used to detect the expression of CTNNB1, ICAM-1, VCAM-1, CXCR4, and CCL18 in RCC cell lines. It was found thatCTNNB1knockdown inhibited cell proliferation, migration, and invasion and induced apoptosis of A-498 cells.CTNNB1overexpression promoted cell proliferation, migration, and invasion and inhibited apoptosis of 786-O cells. Moreover, knockdown ofCTNNB1decreased the levels of ICAM-1, VCAM-1, CXCR4, and CCL18 expression, butCTNNB1overexpression increased the expression of ICAM-1, VCAM-1, CXCR4, and CCL18. Further in vivo tumor formation study in nude mice indicated that inhibition ofCTNNB1delayed the progress of tumor formation through inhibiting PCNA and Ki67 expression. These results indicate thatCTNNB1could act as an oncogene and may serve as a promising therapeutic strategy for RCC.
authors.affiliation: Department of Urology, The First Affiliated Hospital, China Medical University.
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, The Fifth People's Hospital of Shenyang.
authors.forename: Shan
authors.initials: S
authors.lastname: Ji
authors.affiliation: Department of Biotherapy, Cancer Research Institute, The First Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biotherapy, Cancer Research Institute, The First Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Li-Ye
authors.initials: LY
authors.lastname: Fu
authors.affiliation: Department of Biotherapy, Cancer Research Institute, The First Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Biotherapy, Cancer Research Institute, The First Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Fan-Dong
authors.initials: FD
authors.lastname: Meng
keywords: kidney cancer; oncogene; survival time; tumor migration-related protein; β-catenin