title: Glycation of antibodies: Modification, methods and potential effects on biological functions.
abstract: Glycation is an important protein modification that could potentially affect bioactivity and molecular stability, and glycation of therapeutic proteins such as monoclonal antibodies should be well characterized. Glycated protein could undergo further degradation into advance glycation end (AGE) products. Here, we review the root cause of glycation during the manufacturing, storage and in vivo circulation of therapeutic antibodies, and the current analytical methods used to detect and characterize glycation and AGEs, including boronate affinity chromatography, charge-based methods, liquid chromatography-mass spectrometry and colorimetric assay. The biological effects of therapeutic protein glycation and AGEs, which ranged from no affect to loss of activity, are also discussed.
authors.affiliation: a Protein Analytical Chemistry, Genentech , South San Francisco , CA , USA.
authors.forename: Bingchuan
authors.initials: B
authors.lastname: Wei
authors.affiliation: a Protein Analytical Chemistry, Genentech , South San Francisco , CA , USA.
authors.forename: Kelsey
authors.initials: K
authors.lastname: Berning
authors.affiliation: a Protein Analytical Chemistry, Genentech , South San Francisco , CA , USA.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Quan
authors.affiliation: a Protein Analytical Chemistry, Genentech , South San Francisco , CA , USA.
authors.forename: Yonghua Taylor
authors.initials: YT
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000097:blood* / Q000627:therapeutic use*; D002846:Chromatography, Affinity; D003124:Colorimetry; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry; D055550:Protein Stability
chemical_list: D000906:Antibodies; D017127:Glycation End Products, Advanced
keywords: Advanced glycation end products; antibody; bioactivity; glycation; methods; modification