title: Blanching influences the phenolics composition, antioxidant activity, and inhibitory effect ofAdansonia digitataleaves extract onα-amylase,α-glucosidase, and aldose reductase.
abstract: Adansonia digitata(A.digitata) leaves serve as food and has several medicinal uses in many parts of the world. This study evaluated the influence of blanching on the phenolics composition, antioxidant activity, and inhibitory effect of methanol extract ofA.digitataleaves on the activities of some key enzymes (α-amylase,α-glucosidase, and aldose reductase) implicated in type 2 diabetes (T2D) in vitro. Reverse-phase HPLC analysis revealed that the leaves had appreciable levels of flavonoids and phenolic acids, including catechin, epicatechin, rutin, quercitrin, quercetin, kaempferol, and luteolin (flavonoids); gallic, chlorogenic, caffeic, and ellagic acids (phenolic acids). Blanching caused significant (P< 0.05) decrease in the flavonoids and phenolic acids contents; DPPH* (2,2 diphenyl-1-picrylhydrazyl radical) and ABTS*+[2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) radical cation] scavenging ability; reducing power; and Fe2+-induced lipid peroxidation inhibitory capacity of the extract. Similarly, the inhibitory effect of the extract on the activities ofα-amylase,α-glucosidase, and aldose reductase was significantly (P< 0.05) reduced due to blanching. Thus,A.digitataleaves extract could be effective for the management of T2D due to its flavonoids and phenolic acids content, antioxidant properties, and inhibitory potency on the activities ofα-amylase,α-glucosidase, and aldose reductase. However, blanching militated against the levels of these functional attributes of the leaves and, therefore, may not be recommended for their optimal retention.
authors.affiliation: Biochemistry Unit Department of Biosciences and Biotechnology Kwara State University Malete, P.M.B. 1530 Ilorin Nigeria.
authors.forename: Emmanuel A
authors.initials: EA
authors.lastname: Irondi
authors.affiliation: Biochemistry Unit Department of Biosciences and Biotechnology Kwara State University Malete, P.M.B. 1530 Ilorin Nigeria.
authors.forename: Jacob K
authors.initials: JK
authors.lastname: Akintunde
authors.affiliation: Department of Veterinary Physiology, Biochemistry and Pharmacology University of Ibadan Ibadan Nigeria.
authors.forename: Samson O
authors.initials: SO
authors.lastname: Agboola
authors.affiliation: Phytochemical Research Laboratory Department of Industrial Pharmacy Federal University of Santa Maria Building 26, room 1115 Santa Maria CEP 97105-900 Brazil.
authors.forename: Aline A
authors.initials: AA
authors.lastname: Boligon
authors.affiliation: Phytochemical Research Laboratory Department of Industrial Pharmacy Federal University of Santa Maria Building 26, room 1115 Santa Maria CEP 97105-900 Brazil.
authors.forename: Margareth L
authors.initials: ML
authors.lastname: Athayde
keywords: Adansonia digitata leaves; blanching; carbohydrate‐hydrolyzing enzymes; diabetic complications; phenolics; type 2 diabetes