title: Involvement of the miR-462/731 cluster in hypoxia response in Megalobrama amblycephala.
abstract: MicroRNAs (miRNAs) are non-coding small RNAs showing both evolutionarily conserved and unique features and are involved in nearly all biological processes. In the present study, the role played by miR-462/731 cluster miRNAs in hypoxia response in Megalobrama amblycephala, an important freshwater fish, was investigated. The M. amblycephala miR-462/731 cluster locus and their 5' flanking sequences were sequenced and analyzed. In M. amblycephala and other teleost fish species, the mature sequences of miR-462 and miR-731 were identical and hypoxia-responsive elements (HREs) were identified upstream of the miR-462/731 loci. The two miRNAs were significantly induced in the liver, spleen, gill, muscle, and brain after hypoxia treatment. The expression of both miRNAs was also upregulated in cells that received treatment which mimicked hypoxia. Furthermore, reporter assay revealed that M. amblycephala HREs can be activated by hypoxia. Taken together, the 462/731 cluster may play a role in the regulation of the hypoxia response in M. amblycephala.
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Cui-Hong
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Huang
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wu
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, People's Republic of China.|Hubei Provincial Engineering Laboratory for Pond Aquaculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, People's Republic of China.|Hubei Provincial Engineering Laboratory for Pond Aquaculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Tang
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.|Hubei Provincial Engineering Laboratory for Pond Aquaculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China. hbauwhl@hotmail.com.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, People's Republic of China. hbauwhl@hotmail.com.|Hubei Provincial Engineering Laboratory for Pond Aquaculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China. hbauwhl@hotmail.com.
authors.forename: Huan-Ling
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D014867:Water; D010100:Oxygen
keywords: HRE; Hypoxia; Megalobrama amblycephala; miR-462/731 cluster