title: Enzymatic-fluorometric analyses for glutamine, glutamate and free amino groups in protein-free plasma and milk.
abstract: This Technical Research Communication describes new analytical methods for free, unbound glutamic acid and glutamine in protein-free blood plasma and milk and introduces the use of quantitation of free amino groups in the same matrices for descriptive and analytical purposes. The present enzymatic-fluorometric methods are easily performed within one working day, allowing for 'high throughput' assays of animal trials. These assays could support and enable further studies in lactation physiology with the objective of improved metabolic health.
authors.affiliation: Department of Animal Science,Aarhus University,Blichers Allé 20,PO Box 50,8830 Tjele,Denmark.
authors.forename: Torben
authors.initials: T
authors.lastname: Larsen
authors.affiliation: ACUMA Research Center,Polytechnic University of Valencia,46022 Valencia,Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Fernández
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000097:blood*; D000818:Animals; D002417:Cattle; D005260:Female; D005470:Fluorometry / Q000379:methods*; D018698:Glutamic Acid / Q000032:analysis* / Q000097:blood; D005973:Glutamine / Q000032:analysis* / Q000097:blood; D007202:Indicators and Reagents; D007774:Lactation; D008623:Mercaptoethanol / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D009764:o-Phthalaldehyde / Q000737:chemistry
chemical_list: D000596:Amino Acids; D007202:Indicators and Reagents; D005973:Glutamine; D018698:Glutamic Acid; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde
keywords: Gln; Glu; Milk metabolites; OPA; biomarkers