title: Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS).
abstract: Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal-cyanide complexes as the sole nitrogen source. In this work, the strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. To elucidate the molecular mechanisms involved in the bioremediation process, a quantitative proteomic analysis by LC-MS/MS has been carried out in P. pseudoalcaligenes CECT5344 cells grown with the jewelry residue as sole nitrogen source. Different proteins related to cyanide and cyanate assimilation, as well as other proteins involved in transport and resistance to metals were induced by the cyanide-containing jewelry residue. GntR-like regulatory proteins were also induced by this industrial residue and mutational analysis revealed that GntR-like regulatory proteins may play a role in the regulation of cyanide assimilation in P. pseudoalcaligenes CECT5344. The strain CECT5344 has been used in a batch reactor to remove at pH 9 the different forms of cyanide present in industrial wastewaters from the jewelry industry (0.3 g/L, ca. 12 mM total cyanide, including both free cyanide and metal-cyanide complexes). This is the first report describing the biological removal at alkaline pH of such as elevated concentration of cyanide present in a heterogeneous mixture from an industrial source.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Ibáñez
authors.affiliation: Departamento de Botánica, Ecología y Fisiología Vegetal, Edificio Celestino Mutis, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Purificación
authors.initials: P
authors.lastname: Cabello
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Víctor Manuel
authors.initials: VM
authors.lastname: Luque-Almagro
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Lara P
authors.initials: LP
authors.lastname: Sáez
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Olaya
authors.affiliation: Departamento de Química Analítica, Edificio Marie Curie, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Sánchez de Medina
authors.affiliation: Departamento de Química Analítica, Edificio Marie Curie, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Luque de Castro
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: Conrado
authors.initials: C
authors.lastname: Moreno-Vivián
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Roldán
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002853:Chromatography, Liquid / Q000379:methods*; D003486:Cyanides / Q000633:toxicity*; D005798:Genes, Bacterial; D040901:Proteomics; D044205:Pseudomonas pseudoalcaligenes / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D003486:Cyanides; D062065:Wastewater