title: HMOX1 Promoter Microsatellite Polymorphism Is Not Associated With High Altitude Pulmonary Edema in Han Chinese.
abstract: OBJECTIVE
  To investigate the relationship between microsatellite polymorphism in the Heme oxygenase-1 (HMOX1) gene promoter and high-altitude pulmonary edema (HAPE) in Han Chinese.
  
  
  METHODS
  Eighty-three construction workers who developed HAPE 2 to 7 days after arrival at Yushu (3800 m) in Qinghai, China, and 145 matched healthy subjects were included in this study. The amplification and labeling of the polymerase chain reaction products for capillary electrophoresis were performed to identify HMOX1 genotype frequency. The alleles were classified as short (S: <25 [GT]n repeats) and long (L: ≥25 [GT]n repeats) alleles.
  
  
  RESULTS
  Patients with HAPE have significantly higher white blood cell count, heart rate, and mean pulmonary artery pressure, but lower hemoglobin and arterial oxygen saturation than healthy subjects without HAPE. The numbers of (GT)n repeats in the HMOX1 gene promoter show a bimodal distribution. However, there is no significant difference in the genotype frequency and allele frequency between patients with HAPE and healthy subjects without HAPE. Chi-square test analysis reveals that the genotype frequency of (GT)n repeats is not associated with HAPE.
  
  
  CONCLUSIONS
  The microsatellite polymorphism in the HMOX1 gene promoter is not associated with HAPE in Han Chinese in Qinghai, China.
authors.affiliation: Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, China; The Key Laboratory of High Altitude Medicine, Qinghai, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Cao
authors.affiliation: Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, China; The Key Laboratory of High Altitude Medicine, Qinghai, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, China; The Key Laboratory of High Altitude Medicine, Qinghai, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Ma
authors.affiliation: Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, China; The Key Laboratory of High Altitude Medicine, Qinghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, China; The Key Laboratory of High Altitude Medicine, Qinghai, China. Electronic address: geriligao@hotmail.com.
authors.forename: Ri-Li
authors.initials: RL
authors.lastname: Ge
mesh_terms: D000328:Adult; D000532:Altitude Sickness / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D006976:Hypertension, Pulmonary / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
chemical_list: C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1
keywords: (GT)n microsatellite polymorphism; heme oxygenase-1 gene; high altitude pulmonary edema