title: In Vitro Reconstitution of Autophagosome-Lysosome Fusion.
abstract: SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) proteins are a highly regulated class of membrane proteins lying in the center of membrane fusion. In conjunction with accessory proteins, SNAREs drive efficient merger of two distinct lipid bilayers into one interconnected structure. This chapter describes our fluorescence resonance energy transfer (FRET)-based proteoliposome fusion assays for the roles of various SNARE proteins, accessory proteins, and effects of different lipid compositions on membrane fusion involved in autophagy.
authors.affiliation: University of Cincinnati College of Medicine, Cincinnati, OH, United States. Electronic address: jiajie.diao@uc.edu.
authors.forename: J
authors.initials: J
authors.lastname: Diao
authors.affiliation: Center for Autophagy Research, University of Texas Southwestern Medical Center, Dallas, TX, United States; University of Texas Southwestern Medical Center, Dallas, TX, United States.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: Stanford University, Stanford, CA, United States.
authors.forename: Y
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Center for Autophagy Research, University of Texas Southwestern Medical Center, Dallas, TX, United States; University of Texas Southwestern Medical Center, Dallas, TX, United States. Electronic address: qing.zhong@utsouthwestern.edu.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhong
mesh_terms: D000071182:Autophagosomes / Q000378:metabolism*; D001671:Biochemistry / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D050600:SNARE Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D050600:SNARE Proteins
keywords: ATG14; Accessory proteins; Autophagy; Fusion; Lipid; Lysosome; SNARE; Single vesicle