title: Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen.
abstract: This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was pooled and extended by egg yolk based extender supplemented with different concentrations of quercetin (10 or 20 μM) along with 5% glycerol or DMA. The extended semen was frozen and sperm motility parameters, viability, abnormality, membrane integrity and lipid peroxidation were assessed after thawing. Results showed that sperm viability, total motility, progressive motility, straightness (STR) and linearity (LIN) were higher (P < 0.05), and abnormality percentage and MDA concentration were lower (P < 0.05) in extender containing DMA. Similarly, higher (P < 0.05) total motility, progressive motility, viability and membrane integrity along with lower (P < 0.05) MDA level were noted in Q10 group. The lowest (P < 0.05) MDA level was observed in DMA extender containing moderate level of quercetin (Q10D). Also the STR was higher (P < 0.05) in Q10D compared to Q10G and Q20G groups. In conclusion, supplementation of extender with 10 μM quercetin in combination with DMA improves the goat sperm motion kinetics and suppresses lipid peroxidation after freezing and thawing. Furthermore, DMA is more effective cryoprotectant for the freezing of goat sperm.
authors.affiliation: Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Seifi-Jamadi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: Ejaz
authors.initials: E
authors.lastname: Ahmad
authors.affiliation: Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Ansari
authors.affiliation: Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran. Electronic address: Hamid_Kohram@yahoo.com.
authors.forename: Hamid
authors.initials: H
authors.lastname: Kohram
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology; D004530:Egg Yolk; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D006041:Goats; D008297:Male; D011794:Quercetin / Q000494:pharmacology*; D012661:Semen; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D000081:Acetamides; D000975:Antioxidants; D003451:Cryoprotective Agents; D011794:Quercetin; C013959:dimethylacetamide; D005990:Glycerol
keywords: Antioxidant; Malondialdehyde; Quercetin; Sperm; Stallion