title: Allicin protects against cisplatin-induced vestibular dysfunction by inhibiting the apoptotic pathway.
abstract: Cisplatin is an anticancer drug that causes the impairment of inner ear function as side effects, including hearing loss and balance dysfunction. The purpose of this study was to investigate the effects of allicin against cisplatin-induced vestibular dysfunction in mice and to make clear the mechanism underlying the protective effects of allicin on oto-vestibulotoxicity. Mice intraperitoneally injected with cisplatin exhibited vestibular dysfunction in swimming test, which agreed with impairment in vestibule. However, these impairments were significantly prevented by pre-treatment with allicin. Allicin markedly reduced cisplatin-activated expression of cleaved-caspase-3 in hair cells and vascular layer cells of utricule, saccule and ampulla, but also decreased AIF nuclear translocation of hair cells in utricule, saccule and ampulla. These results showed that allicin played an effective role in protecting vestibular dysfunction induced by cisplatin via inhibiting caspase-dependent and caspase-independent apoptotic pathways. Therefore, allicin may be useful in preventing oto-vestibulotoxicity mediated by cisplatin.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, PR China; Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Xianmin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, Jinan, PR China; Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, Jinan, PR China; Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, PR China; Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Otology, Jinan, PR China; Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Otology, Jinan, PR China; Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Bai
authors.affiliation: Shandong Provincial Key Laboratory of Otology, Jinan, PR China; Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Yuechen
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Daogong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China; Shandong Provincial Key Laboratory of Otology, Jinan, PR China; Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China. Electronic address: fanent@126.com.
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Fan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002945:Cisplatin / Q000009:adverse effects*; D004220:Disulfides; D005260:Female; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D013441:Sulfinic Acids / Q000494:pharmacology*; D014722:Vestibule, Labyrinth / Q000187:drug effects* / Q000473:pathology* / Q000503:physiopathology
chemical_list: D004220:Disulfides; D013441:Sulfinic Acids; C006452:allicin; D020169:Caspases; D002945:Cisplatin
keywords: Allicin; Apoptosis; Cisplatin; Ototoxicity; Vestibular dysfunction