title: Complete Nucleotide Sequences of Two VIM-1-Encoding Plasmids from Klebsiella pneumoniae and Leclercia adecarboxylata Isolates of Czech Origin.
abstract: Two multidrug resistance (MDR) plasmids, carrying the VIM-1-encoding integron In110, were characterized. Plasmid pLec-476cz (311,758 bp), from aLeclercia adecarboxylataisolate, consisted of an IncHI1 backbone, a MDR region, and two accessory elements. Plasmid pKpn-431cz (142,876 bp), from a sequence type 323 (ST323)Klebsiella pneumoniaeisolate, comprised IncFIIY-derived and pKPN3-like sequences and a mosaic region. A 40,400-bp sequence of pKpn-431cz was identical to the MDR region of pLec-476cz, indicating theen blocacquisition of the VIM-1-encoding region from one plasmid by the other.
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.|CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Papousek
authors.affiliation: Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University, Plzen, Czech Republic c.papagiannitsis@gmail.com.|Biomedical Center, Faculty of Medicine in Plzen, Charles University, Plzen, Czech Republic.
authors.forename: Costas C
authors.identifier: 0000-0002-9912-9028
authors.initials: CC
authors.lastname: Papagiannitsis
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Matej
authors.initials: M
authors.lastname: Medvecky
authors.affiliation: Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University, Plzen, Czech Republic.|Biomedical Center, Faculty of Medicine in Plzen, Charles University, Plzen, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Hrabak
authors.affiliation: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.|CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Dolejska
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases; C063912:carbapenemase
keywords: IncFIIY; IncHI1; carbapenemases; integrative conjugative elements; metallo-β-lactamases