title: Inferring fitness landscapes and selection on phenotypic states from single-cell genealogical data.
abstract: Recent advances in single-cell time-lapse microscopy have revealed non-genetic heterogeneity and temporal fluctuations of cellular phenotypes. While different phenotypic traits such as abundance of growth-related proteins in single cells may have differential effects on the reproductive success of cells, rigorous experimental quantification of this process has remained elusive due to the complexity of single cell physiology within the context of a proliferating population. We introduce and apply a practical empirical method to quantify the fitness landscapes of arbitrary phenotypic traits, using genealogical data in the form of population lineage trees which can include phenotypic data of various kinds. Our inference methodology for fitness landscapes determines how reproductivity is correlated to cellular phenotypes, and provides a natural generalization of bulk growth rate measures for single-cell histories. Using this technique, we quantify the strength of selection acting on different cellular phenotypic traits within populations, which allows us to determine whether a change in population growth is caused by individual cells' response, selection within a population, or by a mixture of these two processes. By applying these methods to single-cell time-lapse data of growing bacterial populations that express a resistance-conferring protein under antibiotic stress, we show how the distributions, fitness landscapes, and selection strength of single-cell phenotypes are affected by the drug. Our work provides a unified and practical framework for quantitative measurements of fitness landscapes and selection strength for any statistical quantities definable on lineages, and thus elucidates the adaptive significance of phenotypic states in time series data. The method is applicable in diverse fields, from single cell biology to stem cell differentiation and viral evolution.
authors.affiliation: Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: Takashi
authors.identifier: 0000-0003-2556-6484
authors.initials: T
authors.lastname: Nozoe
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, Department of Physics, New York University, New York, New York, United States of America.
authors.forename: Edo
authors.initials: E
authors.lastname: Kussell
authors.affiliation: Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: Yuichi
authors.identifier: 0000-0002-6233-0844
authors.initials: Y
authors.lastname: Wakamoto
mesh_terms: D005075:Biological Evolution; D002453:Cell Cycle; D019070:Cell Lineage; D003198:Computer Simulation; D004777:Environment; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D056084:Genetic Fitness; D008853:Microscopy / Q000379:methods; D010641:Phenotype; D011336:Probability; D012641:Selection, Genetic; D059010:Single-Cell Analysis; D013269:Stochastic Processes