title: Association of CYP2J2 gene polymorphisms with ischemic stroke and stroke subtypes in Chinese population.
abstract: OBJECTIVE
  Ischemic stroke (IS) is the main cause of mortality and disability among the old people in China and is a multifactorial disease influenced by many factors including genetic factors like the allele for CYP 2J2. It has been demonstrated that CYP2J2 polymorphisms alter the transcriptional activity. However, studies on the association between CYP2J2-50G/T polymorphism and IS have reported conflicting results. Thus, our study aimed to examine the association between 4 variants in the CYP2J2 gene and the risk of IS and its subtypes, in the Chinese population.
  
  
  METHODS
  In this study, genotyping was performed by using polymerase chain reaction (PCR) sequencing for 202 IS patients and 206 age- and sex-matched controls. Odds ratios (ORs) and confidence interval (CI) were estimated by multivariate logistic regression and PCR results were confirmed by DNA sequencing. A meta-analysis was conducted to evaluate the association of CYP2J2-50G>T polymorphism with the risk of IS in Chinese population by calculating pooled OR.
  
  
  RESULTS
  We found this polymorphism was significantly associated with IS (17.82% vs. 10.68%, P = 0.039). Multiple logistic regression analysis revealed that GT genotype was associated with a significantly high risk of IS (OR = 2.32, 95% CI: 1.21-4.45, P = 0. 011) after adjustment for other confounding factors such as hypertension, diabetes, heart disease, smoking habit, family history, triglyceride and low-density lipoprotein levels. We also found a significant association of GT genotype with small artery occlusion (SAA) (P < 0.05; OR = 2.22; 95% CI: 1.043-4.72). Meta-analysis results also showed that the GT genotype carriers had a negative effect on the risk of IS in Chinese population with overall OR of 1.40 (95% CI: 1.06-1.84).
  
  
  CONCLUSIONS
  The findings of the present study suggested that polymorphism in -50G/T position of CYP2J2 gene might be a risk factor for IS in Chinese population. Further large prospective studies were required to confirm these findings.
authors.affiliation: Stroke Center, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Wang
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Xing
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Zhang
authors.forename: Ben-Qiang
authors.initials: BQ
authors.lastname: Deng
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D002545:Brain Ischemia / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020521:Stroke / Q000235:genetics*
chemical_list: C000717350:CYP2J2 protein, human; D003577:Cytochrome P-450 Enzyme System; D000090762:Cytochrome P-450 CYP2J2