title: Reactive oxygen species (ROS) and dimethylated sulphur compounds in coral explants under acute thermal stress.
abstract: Coral bleaching is intensifying with global climate change. Although the causes for these catastrophic events are well understood, the cellular mechanism that triggers bleaching is not well established. Our understanding of coral bleaching processes is hindered by the lack of robust methods for studying interactions between host and symbiont at the single-cell level. Here, we exposed coral explants to acute thermal stress and measured oxidative stress, more specifically, reactive oxygen species (ROS), in individual symbiont cells. Furthermore, we measured concentrations of dimethylsulphoniopropionate (DMSP) and dimethylsulphoxide (DMSO) to elucidate the role of these compounds in coral antioxidant function. This work demonstrates the application of coral explants for investigating coral physiology and biochemistry under thermal stress and delivers a new approach to study host-symbiont interactions at the microscale, allowing us to directly link intracellular ROS with DMSP and DMSO dynamics.
authors.affiliation: Climate Change Cluster, University of Technology Sydney, Ultimo, New South Wales 2007, Australia Stephanie.Gardner@student.uts.edu.au.|School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
authors.forename: Stephanie G
authors.identifier: 0000-0001-8359-1835
authors.initials: SG
authors.lastname: Gardner
authors.affiliation: Climate Change Cluster, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Raina
authors.affiliation: Climate Change Cluster, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Ralph
authors.affiliation: School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
authors.forename: Katherina
authors.initials: K
authors.lastname: Petrou
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry / Q000502:physiology*; D000975:Antioxidants / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D004141:Dinoflagellida / Q000737:chemistry / Q000502:physiology*; D006358:Hot Temperature / Q000009:adverse effects; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013452:Sulfonium Compounds / Q000378:metabolism; D013559:Symbiosis
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D013452:Sulfonium Compounds; C068078:dimethylpropiothetin; D004121:Dimethyl Sulfoxide
keywords: Coral explant; Dimethylsulphoniopropionate (DMSP); Fungia granulosa; Reactive oxygen species