title: Long noncoding RNA BLACAT1 indicates a poor prognosis of colorectal cancer and affects cell proliferation by epigenetically silencing of p15.
abstract: Recently, a novel class of transcripts, long noncoding RNAs (lncRNAs), is being identified at a rapid pace. These RNAs have critical roles in diverse biological processes, including tumorigenesis. One of them, BLACAT1, a cancer-associated long noncoding RNA, exerts regulatory functions in various biological processes in cancer cells, however, the role of BLACAT1 in colon cancer remains unclear. Our experiments showed that increased BLACAT1 was an independent unfavorable prognostic indicator for colorectal cancer, and revealed that BLACAT1 knockdown significantly repressed proliferation, both in vitro and in vivo. Mechanistic investigations demonstrated that BLACAT1 had a key role in G1/G0 arrest, and showed that BLACAT1 can repress p15 expression by binding to EZH2, thus contributing to the regulation of CRC cell cycle and proliferation. Our results suggest that BLACAT1, as a cell cycle regulator, may serve as a potential target for colon cancer prevention and treatment in human CRC.
authors.affiliation: Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Epidemiology and Biostatistics, Collaborative Innovation Center For Cancer Personalized Medicine, School of Public Health,Nanjing Medical University, Nanjing, China.|Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center of Cancer Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Erbao
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Xuzhou Central Hospital, Affiliated Xuzhou Hospital, College of Medicine, Southeast University, Xuzhou, Jiangsu, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Han
authors.affiliation: Central Laboratory, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yin
authors.affiliation: Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Clinical Medicine, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Peiyao
authors.initials: P
authors.lastname: Mao
authors.affiliation: Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Mao
authors.affiliation: Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics*; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000235:genetics; D044127:Epigenesis, Genetic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D051379:Mice; D011379:Prognosis; D011485:Protein Binding; D062085:RNA, Long Noncoding / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: D050762:Cyclin-Dependent Kinase Inhibitor p15; D062085:RNA, Long Noncoding; C000624139:long noncoding RNA BLACAT1, human; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein