title: Characterization of Potential Polysaccharide Utilization Systems in the MarineBacteroidetes Gramella FlavaJLT2011 Using a Multi-Omics Approach.
abstract: Members of phylumBacteroidetesare distributed across diverse marine niches andFlavobacteriais often the predominant bacterial class decomposing algae-derived polysaccharides. Here, we report the complete genome ofGramella flavaJLT2011 (Flavobacteria) isolated from surface water of the southeastern Pacific. A remarkable genomic feature is that the number of glycoside hydrolase (GH) genes in the genome ofG. flavaJLT2011 is more than 2-fold higher than that of otherGramellaspecies. The functional profiles of the GHs suggest extensive variation inGramellaspecies. Growth experiments revealed thatG. flavaJLT2011 has the ability to utilize a wide range of polysaccharides for growth such as xylan and homogalacturonan in pectin. Nearly half of all GH genes were located on the multi-gene polysaccharide utilization loci (PUL) or PUL-like systems inG. flavaJLT2011. This species was also found to harbor the two xylan PULs and a pectin PUL, respectively. Gene expression data indicated that more GHs and sugar-specific outer-membranesusC-susDsystems were found in the presence of xylan than in the presence of pectin, suggesting a different strategy for heteropolymeric xylan and homoglacturonan utilization. Multi-omics data (transcriptomics, proteomics, and metabolomics) indicated that xylan PULs and pectin PUL are respectively involved in the catabolism of their corresponding polysaccharides. This work presents a comparison of polysaccharide decomposition within a genus and expands current knowledge on the diversity and function of PULs in marineBacteroidetes, thereby deepening our understanding of their ecological role in polysaccharide remineralization in the marine system.
authors.affiliation: State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Xiamen, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Tang
authors.affiliation: State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Xiamen, China.
authors.forename: Yingfan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Xiamen, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.affiliation: State Key Laboratory for Marine Environmental Science, Institute of Marine Microbes and Ecospheres, Xiamen University Xiamen, China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
keywords: Bacteroidetes; genome; glycoside hydrolases; marine bacteria; microarray; polysaccharide utilization loci; proteomics