title: Characterizing and marker-assisting a novel chili pepper (Capsicum annuum L.) yellow bud mutant with cytoplasmic male sterility.
abstract: Cytoplasmic male sterility (CMS) in pepper is a better way to produce hybrid seeds compared to manual production. We used the two sequence characterized amplified region (SCAR) markers (CRF-SCAR and CMS-SCAR130) in CMS pepper, to identify the genotype. We assembled two CMS yellow bud mutants (YBM; YBM12-A and YBM12-B). This mutation in leaf color is controlled by a single dominant nuclear gene. The aim was to create a new hybrid seed production method that reduces the costs and increases F1hybrid seed purity. The results suggest that the CRF-SCAR and CMS-SCAR130markers can be used together in multiple generations to screen for restorer or maintainer genes. We found the marker linked to the restorer gene (Rf) in the C-line and F1hybrids, as well as partially in the F2generation, whereas it was not found in the sterile YBM12-A or the maintainer line YBM12-B. In the F2population, sterility and fertility segregated at a 3:1 ratio based on the CRF-SCAR marker. A 130 bp fragment was produced in the YBM12-A, F1, and F2populations, suggesting that these lines contained sterile cytoplasm. A 140 bp fragment present in the YBM12-B and C-line indicated that these lines contained normal cytoplasm. In addition, we identified some morphological characters distinguishing sterile and fertile buds and flowers that may be linked to the sterility gene. If more restorer lines are identified, CMS expressing the YBM trait can be used in hybrid seed production.
authors.affiliation: Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province, Jurong, Jiangsu, China.
authors.forename: G S
authors.initials: GS
authors.lastname: Sun
authors.affiliation: Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province, Jurong, Jiangsu, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Dai
authors.affiliation: Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, USA.
authors.forename: P W
authors.initials: PW
authors.lastname: Bosland
authors.affiliation: College of Horticulture, Jinling Institute of Technology, Nanjing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province, Jurong, Jiangsu, China.
authors.forename: C Q
authors.initials: CQ
authors.lastname: Sun
authors.affiliation: Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province, Jurong, Jiangsu, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Zhang
authors.affiliation: Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province, Jurong, Jiangsu, China sgs8725@163.com.|Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, USA sgs8725@163.com.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Ma
mesh_terms: D002212:Capsicum / Q000235:genetics*; D003593:Cytoplasm / Q000235:genetics; D005298:Fertility / Q000235:genetics; D035264:Flowers / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D006824:Hybridization, Genetic; D018519:Meristem / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D000069600:Plant Breeding / Q000379:methods; D051479:Plant Infertility / Q000235:genetics*; D015203:Reproducibility of Results; D012639:Seeds / Q000235:genetics
chemical_list: D005819:Genetic Markers