title: Structure and Assembly of Clathrin Cages.
abstract: The unusual structure of clathrin, combined with its ability to assemble and disassemble rapidly in cells provides a model system for us to learn about the ways in which proteins can contribute mechanically to a functioning cell. In this article, we discuss the structural properties of clathrin cages and the triskelions which assemble to form them. The function of clathrin depends on the structure of these triskelions and the interactions they make both with each other during assembly and with the adaptor protein network that drives coated vesicle formation. The atomic resolution structure of clathrin domains has been revealed by X-ray crystallography while scattering studies have enabled the shape of a triskelion in solution to be deduced. Cryo-electron microscopy maps have shown the secondary structure of entire cages, how individual triskelion legs are arranged to form a cage and enabled some bound adaptor proteins to be located. Cage formation itself is energetically finely balanced and requires specific interactions between triskelion legs to be productive, as biochemical studies and in silico modeling have shown. Theoretical, structural and cell biological investigations over many years have contributed to our knowledge of clathrin structure and assembly. It now remains to determine the precise nature of the interactions which occur between clathrin triskelions, light chain and heavy chain and the adaptor protein network.
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Mary
authors.initials: M
authors.lastname: Halebian
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Kyle
authors.initials: K
authors.lastname: Morris
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK. Corinne.smith@warwick.ac.uk.
authors.forename: Corinne
authors.initials: C
authors.lastname: Smith
mesh_terms: D002966:Clathrin / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray
chemical_list: D002966:Clathrin
keywords: 3D protein structure; AFM; Atomic force microscopy; Coarse grain modeling; Coated vesicle; Cryo-electron microscopy; Endocytosis; Macromolecular assemblies; Membrane trafficking; X-ray crystallography