title: Antioxidant defense during desiccation of the resurrection plant Haberlea rhodopensis.
abstract: Maintaining a strong antioxidant system is essential for preventing drought-induced oxidative stress. Thus, in the present study we investigated the role of some non-enzymic and enzymic antioxidants in desiccation tolerance of Haberlea rhodopensis. The effects of high light upon desiccation on antioxidant capacity was estimated by comparing the response of shade and sun plants. The significant enhancement of the antioxidant capacity at 8% RWC corresponded to an enormous increase in flavonoid content. The important role of ascorbate-glutathione cycle in overcoming oxidative stress during drying of H. rhodopensis was established. The antioxidant capacity increased upon dehydration of both shade and sun plants but some differences in non-enzymatic and enzymatic antioxidants were observed. Investigations on the role of polyphenols in desiccation tolerance are scarce. In the present study the polyphenol profiles (fingerprints) of the resurrection plant Haberlea rhodopensis, including all components of the complex are obtained for the first time. It was clarified that the polyphenol complex of H. rhodopensis includes only two types of glycosides - phenylethanoid glucosides and hispidulin 8-C-glucosides. Upon desiccation the polyphenol content increase and the main role of phenylethanoid glucosides in the protection of H. rhodopensis was revealed.
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113, Bulgaria. Electronic address: katyamg@yahoo.com.
authors.forename: Katya
authors.initials: K
authors.lastname: Georgieva
authors.affiliation: Department of Organic Chemistry, Plovdiv University Paisii Hilendarski, "Tzar Assen II" 24, 4000 Plovdiv, Bulgaria.
authors.forename: Soleya
authors.initials: S
authors.lastname: Dagnon
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113, Bulgaria.
authors.forename: Emiliya
authors.initials: E
authors.lastname: Gesheva
authors.affiliation: Department of Organic Chemistry, Plovdiv University Paisii Hilendarski, "Tzar Assen II" 24, 4000 Plovdiv, Bulgaria.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Bojilov
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113, Bulgaria.
authors.forename: Gergana
authors.initials: G
authors.lastname: Mihailova
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113, Bulgaria.
authors.forename: Snezhana
authors.initials: S
authors.lastname: Doncheva
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D002031:Bulgaria; D003681:Dehydration; D055864:Droughts; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008027:Light; D019684:Magnoliopsida / Q000502:physiology*; D018384:Oxidative Stress; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D059808:Polyphenols / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D059808:Polyphenols; D060387:Ascorbate Peroxidases; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Antioxidant activity; Ascorbate-glutathione cycle; Desiccation tolerance; Polyphenols; Resurrection plants