title: PBMC transcriptome profiles identifies potential candidate genes and functional networks controlling the innate and the adaptive immune response to PRRSV vaccine in Pietrain pig.
abstract: The porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. A synergistic action of the innate and the adaptive immune system of the host is essential for mounting a durable protective immunity through vaccination. Therefore, the current study aimed to investigate the transcriptome profiles of peripheral blood mononuclear cells (PBMCs) to characterize the innate and the adaptive immune response to PRRS Virus (PRRSV) vaccination in Pietrain pigs. The Affymetrix gene chip porcine gene 1.0 ST array was used for the transcriptome profiling of PBMCs collected at immediately before (D0), at one (D1) and 28 days (D28) post PRRSV vaccination with three biological replications. With FDR <0.05 and log2 fold change ±1.5 as cutoff criteria, 295 and 115 transcripts were found to be differentially expressed in PBMCs during the stage of innate and adaptive response, respectively. The microarray expression results were technically validated by qRT-PCR. The gene ontology terms such as viral life cycle, regulation of lymphocyte activation, cytokine activity and inflammatory response were enriched during the innate immunity; cytolysis, T cell mediated cytotoxicity, immunoglobulin production were enriched during adaptive immunity to PRRSV vaccination. Significant enrichment of cytokine-cytokine receptor interaction, signaling by interleukins, signaling by the B cell receptor (BCR), viral mRNA translation, IFN-gamma pathway and AP-1 transcription factor network pathways were indicating the involvement of altered genes in the antiviral defense. Network analysis revealed that four network modules were functionally involved with the transcriptional network of innate immunity, and five modules were linked to adaptive immunity in PBMCs. The innate immune transcriptional network was found to be regulated by LCK, STAT3, ATP5B, UBB and RSP17. While TGFß1, IL7R, RAD21, SP1 and GZMB are likely to be predictive for the adaptive immune transcriptional response to PRRSV vaccine in PBMCs. Results of the current immunogenomics study advances our understanding of PRRS in term of host-vaccine interaction, and thereby contribute to design a rationale for disease control strategy.
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.|Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
authors.forename: Md Aminul
authors.initials: MA
authors.lastname: Islam
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Große-Brinkhaus
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.
authors.forename: Maren Julia
authors.initials: MJ
authors.lastname: Pröll
authors.affiliation: Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.|School of Veterinary Science, University of Queensland, Gatton Campus, QLD 4343, Australia.
authors.forename: Muhammad Jasim
authors.initials: MJ
authors.lastname: Uddin
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.
authors.forename: Sharmin
authors.initials: S
authors.lastname: Aqter Rony
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.
authors.forename: Dawit
authors.initials: D
authors.lastname: Tesfaye
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.
authors.forename: Ernst
authors.initials: E
authors.lastname: Tholen
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.|Teaching and Research Station on Frankenfrost, Faculty of Agriculture, University of Bonn, Königswinter, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hoelker
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.|Teaching and Research Station on Frankenfrost, Faculty of Agriculture, University of Bonn, Königswinter, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Neuhoff
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D007111:Immunity, Cellular / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics / Q000276:immunology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine / Q000235:genetics / Q000276:immunology* / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D059467:Transcriptome; D014611:Vaccination; D014765:Viral Vaccines / Q000627:therapeutic use*
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D014765:Viral Vaccines