title: [Preparation and identification of polyclonal antibody of Streptococcus salivarius 57.I urease].
abstract: OBJECTIVE
  To prepare and identify polyclonal antibody of Streptococcus salivarius 57.I urease.
  
  
  METHODS
  The biggest structural subunit of Streptococcus salivarius 57.I urease, UreC, was obtained by gene clone, IPTG-induced expression, and purification through affinity chromatography. Anti-sera and polyclonal antibody were raised by immunizing rabbits with purified UreC. Western blot was utilized to detect the specific combination of polyclonal antibody with UreC.
  
  
  RESULTS
  Purified UreC protein was prepared and used as antigen to immunize rabbits. Polyclonal antibody was obtained， and Western blot displayed a specific band of the polyclonal antibody with UreC about 62 kD as anticipated.
  
  
  CONCLUSIONS
  Polyclonal antibody against Streptococcus salivarius 57.I urease is obtained, which provides an important tool to explore the function of urease and its relationship with dental caries.
authors.affiliation: Department of Preventive Dentistry, Shanghai Stomatological Hospital. Shanghai 200001, China. E-mail:serene2000@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Dan-Ying
authors.initials: DY
authors.lastname: Tao
authors.forename: Xi-Ping
authors.initials: XP
authors.lastname: Feng
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D015153:Blotting, Western; D003731:Dental Caries; D004926:Escherichia coli; D006801:Humans; D011817:Rabbits; D000070001:Streptococcus salivarius / Q000502:physiology*; D014510:Urease / Q000378:metabolism*
chemical_list: D000906:Antibodies; D014510:Urease