title: MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3.
abstract: A growing body of evidence suggests that microRNA-365 (miR-365) played crucial role in the initiation and development of many types of cancers. However, the biological role of miR-365 in human glioma remains unclear. Herein, the aims of this study were to investigate the role and underlying mechanisms of miR-365 in glioma by a series of in vitro and in vivo experiments. We found that miR-365 was strongly downregulated in malignant glioma tissues and cell lines. Restoration of the expression of miR-365 in glioma cells significantly inhibited cell proliferation, migration and invasion in vitro and tumor growth in vivo. Notably, phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) was proved to be a direct target of miR-365 in glioma cells, and its mRNA expression was inversely correlated with miR-365 expression in clinical glioma tissues. PIK3R3 overexpression in miR-365 expressing cells could rescue proliferation, migration and invasion inhibition of miR-365. In addition, miR-365 was able to inhibit the phosphorylation of AKT and mTOR in vitro and in vivo, which are key participants in the AKT/mTOR pathway. These results suggest that miR-365 functioned as a tumor suppressor in glioma by targeting PIK3R3, suggesting that miR-365 has potential as therapeutic targets for glioma.
authors.affiliation: Department of Radiotherapy, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Nuclear Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Hongguang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology, The People's Republic Hospital of Jilin Province, Changchun, Jilin 130021, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Rao
authors.affiliation: Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*
chemical_list: C546819:MIRN365 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; C523442:PIK3R3 protein, human