title: Rapid sensing of melamine in milk by interference green synthesis of silver nanoparticles.
abstract: A highly sensitive, selective, and rapid interference green synthesis based determination of potential milk adulterant melamine has been reported here. Melamine is a nitrogenous compound added to milk for mimicking proteins, consumption of which leads to kidney stones and renal failures. Melamine interacts with ascorbic acid (AA) through strong hydrogen-bonding interactions, thus resulting in an interference/interruption in the formation of silver (Ag) nanoparticles which was confirmed by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). The corresponding benchmark validations for melamine spiked milk samples were performed using High Performance Liquid Chromatography (HPLC). This interference in the formation of Ag nanoparticles resulted in color change that varies with concentration of melamine, thereby enabling in-situ rapid sensing of melamine from milk to a lower limit of 0.1ppm with a linear correlation coefficient of 0.9908.
authors.affiliation: Department of Instrumentation and Applied Physics, Indian Institute of Science (IISc), Bangalore, Karnataka 560012, India.
authors.forename: S
authors.initials: S
authors.lastname: Varun
authors.affiliation: Department of Instrumentation and Applied Physics, Indian Institute of Science (IISc), Bangalore, Karnataka 560012, India.
authors.forename: S C G
authors.initials: SCG
authors.lastname: Kiruba Daniel
authors.affiliation: Department of Instrumentation and Applied Physics, Indian Institute of Science (IISc), Bangalore, Karnataka 560012, India. Electronic address: saisiva.gorthi@iap.iisc.ernet.in.
authors.forename: Sai Siva
authors.initials: SS
authors.lastname: Gorthi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis; D055772:Green Chemistry Technology; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008892:Milk / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis*
chemical_list: D014227:Triazines; D012834:Silver; C011907:melamine
keywords: Ascorbic acid; Biosensor; Green synthesis; Interference sensing; Melamine