title: Plasma and adipose tissue level of angiopoietin-like 7 (ANGPTL7) are increased in obesity and reduced after physical exercise.
abstract: OBJECTIVE
  ANGPTL7 is a member of the Angiopoietin-like (ANGPTL) protein family that is composed of eight proteins (1-8). Increasing evidence is associating ANGPTL proteins to obesity and insulin resistance. The biological role of ANGPTL7 is yet to be understood except for a recently proposed role in the pathophysiology of glaucoma. This study was designed to shed light on the function of ANGPTL7 in obesity and its modulation by physical exercise as well as its potential association with lipid profile.
  
  
  METHODS
  A total of 144 subjects were enrolled in this study and finished three months of physical exercise. The participants were classified based on their BMI, 82 subjects were non-obese and 62 obese. ANGPTL7 levels in plasma and adipose tissue were measured by ELISA, RT-PCR and immunohistochemistry.
  
  
  RESULTS
  In this study, we showed that ANGPTL7 level was increased in the plasma of obese subjects (1249.05± 130.39 pg/mL) as compared to non-obese (930.34 ± 87.27 pg/mL) (p-Value = 0.032). ANGPTL7 Gene and protein expression levels in adipose tissue also showed over two fold increase. Physical exercise reduced circulating level of ANGPTL7 in the obese subjects to 740.98± 127.18 pg/mL, (p-Value = 0.007). ANGPTL7 expression in adipose tissue was also reduced after exercise. Finally, ANGPTL7 circulating level showed significant association with TG level in the obese subjects (R2 = 0.183, p-Value = 0.03).
  
  
  CONCLUSIONS
  In conclusion, our data shows for the first time that obesity increases the level of ANGPTL7 in both plasma and adipose tissue. Increased expression of ANGPTL7 might play a minor role in the regulation of TG level in obese subjects either directly or through interaction with other ANGPTL protein members. Physical exercise reduced the level of ANGPTL7 highlighting the potential for targeting this protein as a therapeutic target for regulating dyslipidemia.
authors.affiliation: Biochemistry and Molecular Biology Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Mohamed
authors.identifier: http://orcid.org/0000-0001-8357-1252
authors.initials: M
authors.lastname: Abu-Farha
authors.affiliation: Biochemistry and Molecular Biology Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Preethi
authors.initials: P
authors.lastname: Cherian
authors.affiliation: Biochemistry and Molecular Biology Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Irina
authors.initials: I
authors.lastname: Al-Khairi
authors.affiliation: Biochemistry and Molecular Biology Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Dhanya
authors.initials: D
authors.lastname: Madhu
authors.affiliation: Biochemistry and Molecular Biology Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Ali
authors.initials: A
authors.lastname: Tiss
authors.affiliation: Biochemistry and Molecular Biology Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Samia
authors.initials: S
authors.lastname: Warsam
authors.affiliation: Clinical Services Department; Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Asma
authors.initials: A
authors.lastname: Alhubail
authors.affiliation: Tissue Banking Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Devarajan
authors.initials: D
authors.lastname: Sriraman
authors.affiliation: Clinical Services Department; Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Faisal
authors.initials: F
authors.lastname: Al-Refaei
authors.affiliation: Biochemistry and Molecular Biology Unit Dasman Diabetes Institute, Kuwait City, Kuwait.
authors.forename: Jehad
authors.initials: J
authors.lastname: Abubaker
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000091307:Angiopoietin-Like Protein 7; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers; D004797:Enzyme-Linked Immunosorbent Assay; D015444:Exercise; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D010168:Palmitates / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: C530622:ANGPTL7 protein, human; D000091307:Angiopoietin-Like Protein 7; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins; D015415:Biomarkers; D010168:Palmitates