title: Profiling analysis of long non-coding RNAs in early postnatal mouse hearts.
abstract: Mammalian cardiomyocytes undergo a critical hyperplastic-to-hypertrophic growth transition at early postnatal age, which is important in establishing normal physiological function of postnatal hearts. In the current study, we intended to explore the role of long non-coding (lnc) RNAs in this transitional stage. We analyzed lncRNA expression profiles in mouse hearts at postnatal day (P) 1, P7 and P28 via microarray. We identified 1,146 differentially expressed lncRNAs with more than 2.0-fold change when compared the expression profiles of P1 to P7, P1 to P28, and P7 to P28. The neighboring genes of these differentially expressed lncRNAs were mainly involved in DNA replication-associated biological processes. We were particularly interested in one novel cardiac-enriched lncRNA, ENSMUST00000117266, whose expression was dramatically down-regulated from P1 to P28 and was also sensitive to hypoxia, paraquat, and myocardial infarction. Knockdown ENSMUST00000117266 led to a significant increase of neonatal mouse cardiomyocytes in G0/G1 phase and reduction in G2/M phase, suggesting that ENSMUST00000117266 is involved in regulating cardiomyocyte proliferative activity and is likely associated with hyperplastic-to-hypertrophic growth transition. In conclusion, our data have identified a large group of lncRNAs presented in the early postnatal mouse heart. Some of these lncRNAs may have important functions in cardiac hyperplastic-to-hypertrophic growth transition.
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Xiongshan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Han
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Hongqin
authors.initials: H
authors.lastname: Luo
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Pan
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Riley Heart Research Center, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Hanying
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Fangjie
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Lai
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Guan
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Suzhou Institute of Blood and Marrow Transplantation, Soochow University, Suzhou, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Chu
authors.affiliation: Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.|Riley Heart Research Center, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Weinian
authors.initials: W
authors.lastname: Shou
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Youcai
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.|Center of Translational Medicine, College of Pharmacy, Third Military Medical University, Chongqing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000254:growth & development; D000860:Hypoxia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D058977:Molecular Sequence Annotation; D009203:Myocardial Infarction / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D038081:Organogenesis / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000633:toxicity; D062085:RNA, Long Noncoding / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000187:drug effects
chemical_list: D062085:RNA, Long Noncoding; D010269:Paraquat