title: Yeast dynamin Vps1 associates with clathrin to facilitate vesicular trafficking and controls Golgi homeostasis.
abstract: The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains unknown. Here we report that Vps1 arrives at the Golgi after clathrin, and that loss of Vps1 leads to a shift in the cellular localization of clathrin to the late endosome and vacuole, not vice versa. Our two-hybrid-based approach provides evidence that full-length Vps1 and its truncated versions bind to the C-terminal region of the Chc1. Cells lacking both Vps1 and Chc1 displayed more severe defects in carboxypeptidase Y (CPY) sorting at the Golgi than those in Vps1-deficient cells. Further, these Vps1 fragments became dominant-negative for CPY sorting upon overexpression. These results suggest that Vps1 binds to Chc1 and functions together at the Golgi for efficient Golgi-to-endosome membrane trafficking. In addition, we found that Vps1, without the aid of clathrin, plays a role in controlling the number and turnover of late Golgi.
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
authors.forename: Shiva Kumar
authors.initials: SK
authors.lastname: Goud Gadila
authors.affiliation: Division of Biological Sciences, University of Missouri, 105 Tucker Hall, Columbia, MO 65211-7400, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Williams
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
authors.forename: Uma
authors.initials: U
authors.lastname: Saimani
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
authors.forename: Mariel
authors.initials: M
authors.lastname: Delgado Cruz
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
authors.forename: Pelin
authors.initials: P
authors.lastname: Makaraci
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Woodman
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
authors.forename: John C W
authors.initials: JC
authors.lastname: Short
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
authors.forename: Hyoeun
authors.initials: H
authors.lastname: McDermott
authors.affiliation: Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA. Electronic address: kkim@missouristate.edu.
authors.forename: Kyoungtae
authors.initials: K
authors.lastname: Kim
mesh_terms: D002966:Clathrin / Q000378:metabolism*; D034281:Dynamins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006706:Homeostasis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002966:Clathrin; D033921:Vesicular Transport Proteins; D019204:GTP-Binding Proteins; C064553:VPS1 protein, S cerevisiae; D034281:Dynamins
keywords: CPY; Clathrin; Golgi; Trafficking; Vps1