title: An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples.
abstract: Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensitive, and specific detection of FAM134B protein in biological (colon cancer cell extracts) and clinical (serum) samples. The approach utilises a differential pulse voltammetry (DPV) in the presence of the [Fe(CN)6]3-/4-redox system to quantify the FAM134B protein in a two-step strategy that involves (i) initial attachment of FAM134B antibody on the surface of extravidin-modified screen-printed carbon electrode, and (ii) subsequent detection of FAM134B protein present in the biological/clinical samples. The assay system was able to detect FAM134B protein at a concentration down to 10 pg μL-1in phosphate buffered saline (pH 7.4) with a good inter-assay reproducibility (% RSD = <8.64, n = 3). We found excellent sensitivity and specificity for the analysis of FAM134B protein in a panel of colon cancer cell lines and serum samples. Finally, the assay was further validated with ELISA method. We believe that our assay could potentially lead a low-cost alternative to conventional immunological assays for target antigens analysis in point-of-care applications.
authors.affiliation: Cancer Molecular Pathology Laboratory in School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Australia.
authors.forename: Farhadul
authors.initials: F
authors.lastname: Islam
authors.affiliation: Cancer Molecular Pathology Laboratory in School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Australia.|School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia.
authors.forename: Md Hakimul
authors.initials: MH
authors.lastname: Haque
authors.affiliation: School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia.|Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia.
authors.forename: Sharda
authors.initials: S
authors.lastname: Yadav
authors.affiliation: School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia.|Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia.
authors.forename: Md Nazmul
authors.initials: MN
authors.lastname: Islam
authors.affiliation: Cancer Molecular Pathology Laboratory in School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Australia. v.gopalan@griffith.edu.au.
authors.forename: Vinod
authors.initials: V
authors.lastname: Gopalan
authors.affiliation: Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia.
authors.forename: Nam-Trung
authors.identifier: 0000-0003-3626-5361
authors.initials: NT
authors.lastname: Nguyen
authors.affiliation: Cancer Molecular Pathology Laboratory in School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Australia. a.lam@griffith.edu.au.
authors.forename: Alfred K
authors.identifier: 0000-0003-2771-564X
authors.initials: AK
authors.lastname: Lam
authors.affiliation: School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia. m.shiddiky@griffith.edu.au.|Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia. m.shiddiky@griffith.edu.au.
authors.forename: Muhammad J A
authors.initials: MJ
authors.lastname: Shiddiky
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D057230:Limit of Detection; D008565:Membrane Proteins; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C522691:RETREG1 protein, human