title: HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey.
abstract: Human papillomaviruses have been established as a risk factor for invasive carcinoma of the uterine cervix. HPV E6/E7 oncogene expression has recently emerged as a promising biomarker to determine the risk for progression to high-grade cervical lesions. The aim of this study was to evaluate HPV mRNA and DNA detection in samples with abnormal cytology.
  
  
  
  Cervical specimens were obtained at the Department of Obstetrics and Gynecology via cervical brushes during January-October 2011. Liquid-based cytology slides were evaluated according to the 2001 Bethesda System. Cytology specimens from a total of 81 women with abnormal cytology were included. Real-time PCR and NASBA assays were performed to detect HPV DNA and E6/E7 mRNA, respectively.
  
  
  
  HPV DNA was identified in 73 samples (90.1%). HPV E6/E7 mRNA expression was observed in 45 samples (55.6%). A statistically significant difference was observed among cytological diagnosis groups. In 25 patients, a biopsy was performed during the follow-up. HPV DNA was detected in all of these patients. HPV E6/E7 expression was present only in CIN I-III diagnosed patients.
  
  
  
  The E6/E7 mRNA test is a robust indicator of cytological atypia and correlates better with progressive lesions than DNA assays.
authors.affiliation: Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: İpek
authors.initials: İ
authors.lastname: Tüney
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Altay
authors.affiliation: Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Koray
authors.initials: K
authors.lastname: Ergünay
authors.affiliation: Department of Pathology, Cytology Unit, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Sevgen Çelik
authors.initials: SÇ
authors.lastname: Önder
authors.affiliation: Department of Pathology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Alp
authors.initials: A
authors.lastname: Usubütün
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Mehmet Coşkun
authors.initials: MC
authors.lastname: Salman
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors.forename: Gülendam
authors.initials: G
authors.lastname: Bozdayi
authors.affiliation: Department of Biostatistics, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Erdem
authors.initials: E
authors.lastname: Karabulut
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, İstanbul University, İstanbul, Turkey.
authors.forename: Osman Selim
authors.initials: OS
authors.lastname: Badur
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Kunter
authors.initials: K
authors.lastname: Yüce
authors.affiliation: Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Pinar
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D027383:Papillomaviridae / Q000235:genetics* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012367:RNA, Viral