title: Proteome Analysis of Human Perilymph Using an Intraoperative Sampling Method.
abstract: The knowledge about the etiology and pathophysiology of sensorineural hearing loss (SNHL) is still very limited. This study aims at the improvement of understanding different types of SNHL by proteome analysis of human perilymph. Sampling of perilymph was established during inner ear surgeries (cochlear implantation, vestibular schwannoma surgeries), and safety of the sampling method was determined by checking hearing threshold with pure-tone audiometry postoperatively. An in-depth shot-gun proteomics approach was performed to identify cochlear proteins and the individual proteome in perilymph of patients. This method enables the identification and quantification of protein composition of perilymph. The proteome of 41 collected perilymph samples with volumes of 1-12 μL was analyzed by data-dependent acquisition, resulting in overall 878 detected protein groups. At least 203 protein groups were solely identified in perilymph, not in reference samples (serum, cerebrospinal fluid), displaying a specific protein pattern for perilymph. Samples were grouped by patient's age and surgery type, leading to the identification of some proteins specific to particular subgroups. Proteins with different abundances between different sample groups were subjected to classification by gene ontology annotations. The identified proteins might serve as biomarkers to develop tools for noninvasive inner ear diagnostics and to elucidate molecular profiles of SNHL.
authors.affiliation: Department of Otolaryngology, Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.|Cluster of Excellence of the German Research Foundation (DFG; "Deutsche Forschungsgemeinschaft") "Hearing4all", Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Heike A
authors.identifier: 0000-0002-1699-7832
authors.initials: HA
authors.lastname: Schmitt
authors.affiliation: Core Facility Proteomics, Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Pich
authors.affiliation: Core Facility Proteomics, Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Schröder
authors.affiliation: Department of Otolaryngology, Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.|Cluster of Excellence of the German Research Foundation (DFG; "Deutsche Forschungsgemeinschaft") "Hearing4all", Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Scheper
authors.affiliation: Department of Otolaryngology, Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.|Cluster of Excellence of the German Research Foundation (DFG; "Deutsche Forschungsgemeinschaft") "Hearing4all", Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Lilli
authors.affiliation: Department of Otolaryngology, Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.|Cluster of Excellence of the German Research Foundation (DFG; "Deutsche Forschungsgemeinschaft") "Hearing4all", Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Reuter
authors.affiliation: Department of Otolaryngology, Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.|Cluster of Excellence of the German Research Foundation (DFG; "Deutsche Forschungsgemeinschaft") "Hearing4all", Hannover Medical School , Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lenarz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D003051:Cochlea / Q000737:chemistry*; D063990:Gene Ontology; D006319:Hearing Loss, Sensorineural; D006801:Humans; D007223:Infant; D008875:Middle Aged; D010498:Perilymph / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000145:classification; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012494:Sampling Studies
chemical_list: D011506:Proteins; D020543:Proteome
keywords: data-dependent acquisition; diagnostic proteomics; inner ear; perilymph