title: Age-Dependent Allergic Asthma Development and Cystathionine Gamma-Lyase Deficiency.
abstract: OBJECTIVE
  The pathogenic mechanisms for the higher prevalence of allergic asthma in children than in adults have not been settled. The aim of the present study is to examine whether the age-dependent development of allergic asthma is caused by age-dependent expression of cystathionine gamma-lyase (CSE), a key enzyme that catalyzes the production of hydrogen sulfide (H2S).
  
  
  RESULTS
  Allergic asthma was induced with ovalbumin in wild-type (WT) and CSE knock-out (KO) mice at young and old ages. CSE expression and H2S production were lower in immune cells of young WT mice than in those of old WT mice. Coincidentally, more severe asthmatic symptoms with a greater type-2 immunoreaction were found in young WT mice than old WT mice. H2S supplementation reversed the asthmatic symptoms. Lower expression levels of CSE proteins were also found in human umbilical cord blood mononuclear cells in comparison with that of peripheral blood mononuclear cells from adult people. The age-dependent asthma propensity vanished in CSE-KO mice, but these mice developed more severe asthma than WT mice. More splenocytes were differentiated to type-2 cytokine-generating cells in young WT mice and in CSE-KO mice at all ages. This differentiation was inhibited by H2S donors. GATA3 translocation to the nucleus and type-2 immunoreaction of splenocytes were inhibited after GATA3 was S-sulfhydrated by H2S. Innovation and Conclusion: For the first time, this study demonstrated that lower abundance of CSE expression and H2S production enhances type-2 immunoreaction and renders a higher incidence of allergic asthma at a young age. As such, H2S level may be a biomarker for asthma development and a H2S-based strategy can be perceived for asthma prevention and treatment. Antioxid. Redox Signal. 27, 931-944.
authors.affiliation: 1 Department of Biology, Lakehead University , Thunder Bay, Canada .
authors.forename: Peipei
authors.initials: P
authors.lastname: Wang
authors.affiliation: 2 Health Sciences North Research Institute , Sudbury, Canada .|3 School of Human Kinetics, Laurentian University , Sudbury, Canada .
authors.forename: Lingyun
authors.initials: L
authors.lastname: Wu
authors.affiliation: 4 School of Kinesiology, Lakehead University , Thunder Bay, Canada .
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Ju
authors.affiliation: 3 School of Human Kinetics, Laurentian University , Sudbury, Canada .
authors.forename: Ming
authors.initials: M
authors.lastname: Fu
authors.affiliation: 1 Department of Biology, Lakehead University , Thunder Bay, Canada .|5 Cardiovascular and Metabolic Research Unit, Laurentian University , Sudbury, Canada .
authors.forename: Tian
authors.initials: T
authors.lastname: Shuang
authors.affiliation: 6 Laboratory of Neuropharmacology, Fudan University School of Pharmacy , Shanghai, China .
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Qian
authors.affiliation: 1 Department of Biology, Lakehead University , Thunder Bay, Canada .|5 Cardiovascular and Metabolic Research Unit, Laurentian University , Sudbury, Canada .
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D000367:Age Factors; D000818:Animals; D001249:Asthma / Q000139:chemically induced* / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D003542:Cystathionine gamma-Lyase / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010047:Ovalbumin / Q000009:adverse effects*
chemical_list: D016207:Cytokines; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D010047:Ovalbumin; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide
keywords: H2S; S-sulfhydration; asthma; gasotransmitter; type-2 immune response