title: Luminescence sensitization of Tb3+-DNA complexes by Ag.
abstract: Terbium ions (Tb3+) with unique photophysical properties have been utilized to develop biosensors with low background and high sensitivity. In this study, the Ag+-sensitized luminescence of Tb3+-DNA complexes was uncovered. The luminescence of Tb3+-DNA complexes could be enhanced by more than 30 times in the presence of Ag+, when Tb3+was bound with poly(G) and poly(T) whereas not with other homopolymers. This research confirmed that the sensitization resulted from the interaction of Ag+with certain bases involved in DNA, not just with the reported certain G-quadruplex sequence. The coordination of Ag+to guanine and thymine bases was expected to increase their rigidities, form Tb3+-DNA-Ag+ternary structures, and thus enhance energy transfer from guanine and thymine to Tb3+. These findings benefit the development of sensitive luminescence probes for various nucleic acids-related targets.
authors.affiliation: CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xu
authors.affiliation: CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Public Health of Guangxi Medical University, Nanning 530021, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China; School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Shen
authors.affiliation: CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Jine
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. Electronic address: rjpei2011@sinano.ac.cn.
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D015374:Biosensing Techniques; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013725:Terbium / Q000737:chemistry*
chemical_list: D049408:Luminescent Agents; D013725:Terbium; D012834:Silver; D004247:DNA
keywords: DNA; Luminescence; Silver; Terbium