title: Bifidobacterium breve with α-linolenic acid alters the composition, distribution and transcription factor activity associated with metabolism and absorption of fat.
abstract: This study focused on the mechanisms that fatty acid conjugating strains - Bifidobacterium breve NCIMB 702258 and Bifidobacterium breve DPC 6330 - influence lipid metabolism when ingested with α-linolenic acid (ALA) enriched diet. Four groups of BALB/c mice received ALA enriched diet (3% (w/w)) either alone or in combination with B. breve NCIMB 702258 or B. breve DPC 6330 (109CFU/day) or unsupplemented control diet for six weeks. The overall n-3 PUFA score was increased in all groups receiving the ALA enriched diet. Hepatic peroxisomal beta oxidation increased following supplementation of the ALA enriched diet with B. breve (P < 0.05) and so the ability of the strains to produce c9t11 conjugated linoleic acid (CLA) was identified in adipose tissue. Furthermore, a strain specific effect of B. breve NCIMB 702258 was found on the endocannabinoid system (ECS). Liver triglycerides (TAG) were reduced following ALA supplementation, compared with unsupplemented controls (P < 0.01) while intervention with B. breve further reduced liver TAG (P < 0.01), compared with the ALA enriched control. These data indicate that the interactions of the gut microbiota with fatty acid metabolism directly affect host health by modulating n-3 PUFA score and the ECS.
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.|Teagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Cork, Ireland.
authors.forename: Elaine
authors.initials: E
authors.lastname: Patterson
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.|Teagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Cork, Ireland.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wall
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, CA 09042, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Lisai
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.|Department of Psychiatry and Neurobehavioural Science, University College Cork, Cork, Ireland.
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Dinan
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.|Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland.
authors.forename: John F
authors.initials: JF
authors.lastname: Cryan
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.|School of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, CA 09042, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Banni
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.
authors.forename: Eamonn M
authors.initials: EM
authors.lastname: Quigley
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.
authors.forename: Fergus
authors.initials: F
authors.lastname: Shanahan
authors.affiliation: APC Microbiome Institute, Biosciences Building, University College Cork, Cork, Ireland.|Teagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Cork, Ireland.
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
mesh_terms: D000818:Animals; D000069984:Bifidobacterium breve / Q000378:metabolism*; D004032:Diet / Q000379:methods*; D050356:Lipid Metabolism; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000008:administration & dosage*; D017962:alpha-Linolenic Acid / Q000008:administration & dosage*
chemical_list: D017962:alpha-Linolenic Acid