title: Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium.
abstract: The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.
authors.affiliation: Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry and Environment Science, New Jersey Institute of Technology, Newark, NJ 07102, USA.
authors.forename: Mengyan
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China.
authors.forename: Longzhen
authors.initials: L
authors.lastname: Ding
authors.affiliation: Department of Civil and Ecological Engineering, I-Shou University, Kaohsiung City 84008, Taiwan. Electronic address: chweng@isu.edu.tw.
authors.forename: Chih-Huang
authors.initials: CH
authors.lastname: Weng
authors.affiliation: Department of Marine Environment Engineering, National Kaohsiung Marine University, Kaohsiung 81157, Taiwan.
authors.forename: Cheng-Di
authors.initials: CD
authors.lastname: Dong
mesh_terms: D001673:Biodegradation, Environmental; D041862:Chloroflexi; D059013:Microbial Consortia; D010100:Oxygen; D012336:RNA, Ribosomal, 16S; D014241:Trichloroethylene; D014752:Vinyl Chloride
chemical_list: D012336:RNA, Ribosomal, 16S; D014241:Trichloroethylene; D010100:Oxygen; D014752:Vinyl Chloride
keywords: Dehalococcoides mccartyi; Non-dechlorinators; Oxygen; Trichloroethylene