title: Effective length calibration method for processing the fluorescence signal detected by charge-coupled device in capillary electrophoresis.
abstract: A novel method named effective length calibration method has been developed to process the fluorescence signal detected by charge-coupled device during capillary electrophoresis. The new method treated each pixel as an individual point detector, and effectively binned a large number of pixels into a final electropherogram without losing the narrow detection window defined by a single pixel. Capillary electrophoresis separations of DNA were carried out and detected by charge-coupled device and conventional detector (photomultiplier tube). Detection properties including signal-to-noise ratio, peak width, detection frequency, and tilt of detector were investigated. It was found that the new method achieved much higher signal-to-noise ratio and smaller peak width than the conventional detector did. A Detection width of 0.5 μm was easily achieved.
authors.affiliation: Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, P.R. China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, P.R. China.
authors.forename: Qinmiao
authors.initials: Q
authors.lastname: Chen
authors.affiliation: School of Optoelectronic Engineering, ChangZhou Institute of Technology, Changzhou, Jiangsu, P.R. China.
authors.forename: Xifang
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, P.R. China.|School of Optoelectronic Engineering, ChangZhou Institute of Technology, Changzhou, Jiangsu, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Dou
mesh_terms: D002138:Calibration; D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary; D005453:Fluorescence
chemical_list: D004247:DNA
keywords: capillary electrophoresis; charge-coupled device; effective-length-calibration; fluorescence detection