title: Genome Mining of Streptomyces mobaraensis DSM40847 as a Bleomycin Producer Providing a Biotechnology Platform To Engineer Designer Bleomycin Analogues.
abstract: Streptomyces mobaraensis DSM40847 has been identified by genome mining and confirmed to be a new bleomycin (BLM) producer. Manipulation of BLM biosynthesis in S. mobaraensis has been demonstrated, as exemplified by the engineered production of 6'-deoxy-BLM A2, providing a biotechnology platform for BLM biosynthesis and engineering. Comparison of DNA cleavage efficiency and kinetics among 6'-deoxy-BLM A2 and selected analogues supports the wisdom of altering the disaccharide moiety to fine-tune BLM activity.
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.lastname: Hindra
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.forename: Dong
authors.identifier: 0000-0003-2917-0663
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Teng
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.forename: Liao-Bin
authors.initials: LB
authors.lastname: Dong
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.forename: Ivana
authors.initials: I
authors.lastname: Crnovčić
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.forename: Huiming
authors.initials: H
authors.lastname: Ge
authors.affiliation: Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
authors.forename: Ben
authors.identifier: 0000-0002-9750-5982
authors.initials: B
authors.lastname: Shen
mesh_terms: D053898:Biosynthetic Pathways; D001761:Bleomycin / Q000096:biosynthesis*; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry; D053837:DNA Cleavage; D030541:Databases, Genetic; D004187:Disaccharides / Q000737:chemistry; D016680:Genome, Bacterial; D015394:Molecular Structure; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D001761:Bleomycin; D004247:DNA