title: Distinct Roles of Met and Interacting Proteins on the Expressions oftakeoutFamily Genes in Brown Planthopper.
abstract: Thetakeoutfamily genes encode relatively small proteins that are related to olfaction and are regulated by juvenile hormone (JH). Thetakeoutgenes modulate various physiological processes, such as behavioral plasticity in the migratory locustLocusta migraloriaand feeding and courtship behaviors inDrosophila. Therefore, to understand the regulatory mechanism of these physiological processes, it is important to study the expressions of thetakeoutgenes that are regulated by JH signaling. We used quantitative real-time PCR (qRTPCR) to study the role of JH signaling in the regulation of thetakeoutfamily genes in the brown planthopperNilaparvata lugens(N. lugens) through the application of Juvenile hormone III (JHIII) and the down-regulation of key genes in the JH signaling pathway. The topical application of JHIII induced the expressions of most of thetakeoutfamily genes, and their expressions decreased 2 and 3 days after the JHIII application. Down-regulating the brown planthopper JH receptor NlMethoprene-tolerant (NlMet) and its interacting partners, NlTaiman (NlTai) and Nlß-Ftz-F1 (Nlß-Ftz), through RNAi, exhibited distinct effects on the expressions of thetakeoutfamily genes. The down-regulation ofNlMetandNlKrüppel-homolog 1(NlKr-h1) increased the expressions of thetakeoutfamily genes, while the down-regulation of the Met interacting partnersNlTaiandNlß-Ftzdecreased the expressions of most of thetakeoutfamily genes. This work advanced our understanding of the molecular function and the regulatory mechanism of JH signaling.
authors.affiliation: Department of Biology, College of Life Sciences, China Jiliang University Hangzhou, China.
authors.forename: Xinda
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Biology, College of Life Sciences, China Jiliang University Hangzhou, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biology, College of Life Sciences, China Jiliang University Hangzhou, China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Jiang
keywords: Met; Taiman; brown planthopper; juvenile hormone; takeout; ß-Ftz-F1