title: Expression of microRNA-122 and microRNA-22 in HBV-related liver cancer and the correlation with clinical features.
abstract: MicroRNAs (miR) participate in cell proliferation, apoptosis and transformation, as they can regulate gene expression and intracellular signal transduction for various physiological processes. MiR-122 and miR-22 are known to be related with occurrence and progression of hepatitis B virus (HBV)-related hepatocellular cancer (HCC). This study recruited HBV-related HCC patients, whose expression levels of miR-122 and miR-22 were determined to analyze the correlation with clinical and pathological indexes.
  
  
  
  HBV-related HCC patients were enrolled, in parallel with patients suffering from benign liver disease and non-HBV-related HCC. Real-time PCR was employed to measure miR-122 and miR-22 expression levels.
  
  
  
  The relative expression levels of miR-122 and miR-22 in HBV-related HCC patients were 1.26 ± 2.73 and 5.49 ± 3.91, respectively, which were significantly lower than that in benign liver disease or non-HBV-related HCC patients (p < 0.05). No significant difference of serum miR-122 or miR-22 levels was found between benign liver disease and non-HBV-related HCC patients (p > 0.05). The miR-122 and miR-22 levels were negatively correlated with tumor size, lymph node metastasis, TNM stage, pathological type, differentiation grade, liver cirrhosis, AFP and HBV DNA, all of which were independent risk factors (p < 0.05).
  
  
  
  MiR-122 and miR-22 were downregulated in HBV-related HCC patients, and were related with tumor size, lymph node metastasis, TNM stage, pathological type, differentiation grade, liver cirrhosis, AFP and HBV DNA.
authors.affiliation: Shandong Provincial Mental Health Center, Jinan, China. hongliyang588@sina.com.
authors.forename: D-D
authors.initials: DD
authors.lastname: Qiao
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: X-F
authors.initials: XF
authors.lastname: Lei
authors.forename: G-L
authors.initials: GL
authors.lastname: Mi
authors.forename: S-L
authors.initials: SL
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: C-Q
authors.initials: CQ
authors.lastname: Xu
authors.forename: H-L
authors.initials: HL
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D005260:Female; D006509:Hepatitis B / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D006515:Hepatitis B virus; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged
chemical_list: C531907:MIRN122 microRNA, human; C548375:MIRN22 microRNA, human; D035683:MicroRNAs