title: A novel route to prepare a multilayer system via the combination of interface-mediated catalytic chain transfer polymerization and thiol-ene click chemistry.
abstract: Herein, we have designed a novel multilayer system composed of poly(methyl methacrylate) [poly(MMA)] brush, biotin, streptavidin and protein-A on a silicon substrate to attach onanti-immunoglobulin G (anti-IgG). poly(MMA) brush with vinyl end-group was first synthesized by the interface-mediated catalytic chain transfer polymerization. The brush was then modified with cysteamine molecules to generate the polymer chains with amine end-group via a thiol-ene click chemistry. The amine end-groups of poly(MMA) chains were also modified with biotin units to ensure selective connection points for streptavidin molecules. Finally, a multilayer system on the silicon substrate was formed by using streptavidin and protein-A molecules, respectively. This multilayer system was employed to attach anti-IgG molecules in a highly oriented manner and provide anti-IgG molecular functional configuration on the multilayer. High reproducibility of the amount of anti-IgG adsorption and homogeneous anti-IgG adsorption layer on the silicon surface could be provided by this multilayer system. The multilayer system with protein A may be opened the door for designing an efficient immunoassay protein chip.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering and Architecture, Yuzuncu Yil University, TR-65080 Van, Turkey.
authors.forename: Adem
authors.initials: A
authors.lastname: Zengin
authors.affiliation: Department of Chemistry, Faculty of Science, Gazi University, 06500 Besevler, Ankara, Turkey. Electronic address: caykara@gazi.edu.tr.
authors.forename: Tuncer
authors.initials: T
authors.lastname: Caykara
mesh_terms: D000327:Adsorption; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D057930:Click Chemistry; D007074:Immunoglobulin G / Q000276:immunology; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D017641:Zeolites / Q000737:chemistry
chemical_list: D007074:Immunoglobulin G; D011108:Polymers; D011109:Polymethacrylic Acids; D013205:Staphylococcal Protein A; D013438:Sulfhydryl Compounds; D017641:Zeolites; D001710:Biotin; D019809:Streptavidin
keywords: Anti-immunoglobulin G; Poly(methyl methacrylate) brushes; Surface-mediated catalytic chain transfer polymerization; Thiol-ene click chemistry