title: Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM.
abstract: Nucleic acid aptamers possess attractive features such as specific molecular recognition, high-affinity binding, and rapid acquisition and replication, which could be feasible components for separating specific cells from other cell types. This study demonstrates that aptamers conjugated to an oligopeptide self-assembled monolayer (SAM) can be used to selectively trap human hepatic cancer cells from cell mixtures containing normal human hepatocytes or human fibroblasts. Molecular dynamics calculations have been performed to understand how the configurations of the aptamers are related to the experimental results of selective cell capture. We further demonstrate that the captured hepatic cancer cells can be detached and collected along with electrochemical desorption of the oligopeptide SAM, and by repeating these catch-and-release processes, target cells can be enriched. This combination of capture with aptamers and detachment with electrochemical reactions is a promising tool in various research fields ranging from basic cancer research to tissue engineering applications.
authors.affiliation: Graduate School of Engineering, Yokohama National University, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Enomoto
authors.affiliation: Graduate School of Engineering, Yokohama National University, Japan.
authors.forename: Tatsuto
authors.initials: T
authors.lastname: Kageyama
authors.affiliation: Graduate School of Engineering, Yokohama National University, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Department of Electronics, Information and Bioengineering, Politecnico di Milano, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Bonalumi
authors.affiliation: Department of Electronics, Information and Bioengineering, Politecnico di Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Marchese
authors.affiliation: Department of Electronics, Information and Bioengineering, Politecnico di Milano, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Gautieri
authors.affiliation: Department of Chemistry, Politecnico di Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Bianchi
authors.affiliation: Department of Chemistry, Politecnico di Milan, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Dubini
authors.affiliation: Cell and Tissue Engineering Laboratory, IRCCS Galeazzi Orthopaedic Institute, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Arrigoni
authors.affiliation: Cell and Tissue Engineering Laboratory, IRCCS Galeazzi Orthopaedic Institute, Italy.|Regenerative Medicine Technologies Lab, Ente Ospedaliero Cantonale, Switzerland.|Swiss Institute for Regenerative Medicine, Switzerland.|Cardiocentro Ticino, Switzerland.
authors.forename: Matteo
authors.initials: M
authors.lastname: Moretti
authors.affiliation: Graduate School of Engineering, Yokohama National University, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Fukuda
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D055664:Electrochemical Techniques; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D009842:Oligopeptides