title: Bioremediation model for atrazine contaminated agricultural soils using phytoremediation (using Phaseolus vulgaris L.) and a locally adapted microbial consortium.
abstract: The objective of the present study was to examine a biological model under greenhouse conditions for the bioremediation of atrazine contaminated soils. The model consisted in a combination of phytoremediation (using Phaseolus vulgaris L.) and rhizopheric bio-augmentation using native Trichoderma sp., and Rhizobium sp. microorganisms that showed no inhibitory growth at 10,000 mg L-1of herbicide concentration. 33.3 mg of atrazine 50 g-1of soil of initial concentration was used and an initial inoculation of 1 × 109UFC mL-1of Rhizobium sp. and 1 × 105conidia mL-1of Trichoderma sp. were set. Four treatments were arranged: Bean + Trichoderma sp. (B+T); Bean + Rhizobium sp. (BR); Bean + Rhizobium sp. + Trichoderma sp. (B+R+T) and Bean (B). 25.51 mg of atrazine 50 g-1of soil (76.63%) was removed by the B+T treatment in 40 days (a = 0.050, Tukey). This last indicate that the proposed biological model and methodology developed is useful for atrazine contaminated bioremediation agricultural soils, which can contribute to reduce the effects of agrochemical abuse.
authors.affiliation: a Institute of Agricultural Sciences, Agronomy and Forestry Area, Universidad Autónoma del Estado de Hidalgo , Tulancingo , Hidalgo , Mexico.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Madariaga-Navarrete
authors.affiliation: a Institute of Agricultural Sciences, Agronomy and Forestry Area, Universidad Autónoma del Estado de Hidalgo , Tulancingo , Hidalgo , Mexico.
authors.forename: Blanca Rosa
authors.initials: BR
authors.lastname: Rodríguez-Pastrana
authors.affiliation: b Academic Area of Chemistry, Institute of Basic Science and Engineering, Universidad Autónoma del Estado de Hidalgo , Pachuca , Hidalgo , Mexico.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Villagómez-Ibarra
authors.affiliation: a Institute of Agricultural Sciences, Agronomy and Forestry Area, Universidad Autónoma del Estado de Hidalgo , Tulancingo , Hidalgo , Mexico.
authors.forename: Otilio Arturo
authors.initials: OA
authors.lastname: Acevedo-Sandoval
authors.affiliation: c Department of Plant Agriculture , Ontario Agricultural College, University of Guelph , Guelph , Ontario , Canada.
authors.forename: Gregory
authors.initials: G
authors.lastname: Perry
authors.affiliation: a Institute of Agricultural Sciences, Agronomy and Forestry Area, Universidad Autónoma del Estado de Hidalgo , Tulancingo , Hidalgo , Mexico.
authors.forename: Margarita
authors.initials: M
authors.lastname: Islas-Pelcastre
mesh_terms: D000383:Agriculture; D001280:Atrazine / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006540:Herbicides / Q000032:analysis / Q000378:metabolism; D008800:Mexico; D059013:Microbial Consortia; D008954:Models, Biological; D027805:Phaseolus / Q000187:drug effects / Q000502:physiology*; D012231:Rhizobium / Q000187:drug effects / Q000378:metabolism; D058441:Rhizosphere; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D014242:Trichoderma / Q000187:drug effects / Q000378:metabolism
chemical_list: D006540:Herbicides; D012989:Soil Pollutants; D001280:Atrazine
keywords: Atrazine degradation; bioremediation; locally adapted microorganism consortium; phytoremediation