title: Effects of acidic pH on voltage-gated ion channels in rat trigeminal mesencephalic nucleus neurons.
abstract: The effects of acidic pH on several voltage-dependent ion channels, such as voltage-dependent K+and Ca2+channels, and hyperpolarization-gated and cyclic nucleotide-activated cation (HCN) channels, were examined using a whole-cell patch clamp technique on mechanically isolated rat mesencephalic trigeminal nucleus neurons. The application of a pH 6.5 solution had no effect on the peak amplitude of voltage-dependent K+currents. A pH 6.0 solution slightly, but significantly inhibited the peak amplitude of voltage-dependent K+currents. The pH 6.0 also shifted both the current-voltage and conductance-voltage relationships to the depolarization range. The application of a pH 6.5 solution scarcely affected the peak amplitude of membrane currents mediated by HCN channels, which were profoundly inhibited by the general HCN channel blocker Cs+(1 mM). However, the pH 6.0 solution slightly, but significantly inhibited the peak amplitude of HCN-mediated currents. Although the pH 6.0 solution showed complex modulation of the current-voltage and conductance-voltage relationships, the midpoint voltages for the activation of HCN channels were not changed by acidic pH. On the other hand, voltage-dependent Ca2+channels were significantly inhibited by an acidic pH. The application of an acidic pH solution significantly shifted the current-voltage and conductance-voltage relationships to the depolarization range. The modulation of several voltage-dependent ion channels by an acidic pH might affect the excitability of mesencephalic trigeminal nucleus neurons, and thus physiological functions mediated by the mesencephalic trigeminal nucleus could be affected in acidic pH conditions.
authors.affiliation: Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.
authors.forename: Jin-Eon
authors.initials: JE
authors.lastname: Han
authors.affiliation: Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.
authors.forename: Jin-Hwa
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.
authors.forename: In-Sun
authors.initials: IS
authors.lastname: Choi
authors.affiliation: Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.; Brain Science & Engineering Institute, Kyungpook National University, Daegu 41940, Korea.
authors.forename: Do-Yeon
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.; Brain Science & Engineering Institute, Kyungpook National University, Daegu 41940, Korea.
authors.forename: Il-Sung
authors.initials: IS
authors.lastname: Jang
keywords: Acidosis; Patch clamp; Proprioception; Trigeminal mesencephalic nucleus; Voltage-gated ion channels