title: Effect of lead on physiological and antioxidant responses in two Vigna unguiculata cultivars differing in Pb-accumulation.
abstract: Lead (Pb) is one of the most toxic anthropogenic pollutants, occurring widely in both terrestrial and aquatic ecosystems, where it impairs plant growth and development. In this work, the effect of 0.5 mM EDTA-Pb was evaluated in two Vigna unguiculata cultivars (SV and SET), with the aim of detecting genotype/cultivar dependent changes in the physiological and anti-oxidant responses (CAT and APX) of a leguminous plant. The data showed that SV accumulated more Pb in roots while SET accumulated more in leaves, indicating differential regulation in Pb-translocation/accumulation. Lead affected the growth of SV less severely than SET, mainly associated with reduced inhibition in photosynthetic parameters. Furthermore, CAT and APX activities increased or were sustained at elevated levels in both cultivars in response to lead. However, gene expression analyses revealed that CAT1 was the main lead responsive gene in SET while CAT2 was more responsive in SV. APX1 was higher expressed in tissues with higher Pb-accumulation while APX2 was ubiquitously responsive to lead in both cultivars. Taken together, these results reveal differential ability of V. unguiculata cultivars in Pb-accumulation in different tissues affecting distinctly physiological and anti-oxidant responses. In addition, the existence of cultivars with predominant Pb-accumulation in aerial tissues invokes a need for studies to identify pollution-safe cultivars of leguminous plants to ensure food safety.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Nila Maria
authors.initials: NM
authors.lastname: Bezerril Fontenele
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil; Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Maria de Lourdes Oliveira
authors.initials: MLO
authors.lastname: Otoch
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Neuza Félix
authors.initials: NF
authors.lastname: Gomes-Rochette
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil; Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Alana Cecília de Menezes
authors.initials: ACM
authors.lastname: Sobreira
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Adolph Annderson Gonçalves Costa
authors.initials: AAGC
authors.lastname: Barreto
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Francisco Dalton Barreto
authors.initials: FDB
authors.lastname: de Oliveira
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: José Hélio
authors.initials: JH
authors.lastname: Costa
authors.affiliation: Departamento de Química Analítica e Físico-Química, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Simone da Silveira Sá
authors.initials: SDSS
authors.lastname: Borges
authors.affiliation: Departamento de Química Analítica e Físico-Química, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
authors.forename: Ronaldo Ferreira
authors.initials: RF
authors.lastname: do Nascimento
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil. Electronic address: fernandesdemelod@gmail.com.
authors.forename: Dirce
authors.initials: D
authors.lastname: Fernandes de Melo
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D007854:Lead / Q000032:analysis* / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism; D013045:Species Specificity; D000070658:Vigna / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000975:Antioxidants; D012989:Soil Pollutants; D007854:Lead
keywords: Antioxidant enzymes; Lead; Vigna unguiculata