title: [Sirtuin type 1 signaling pathway mediates the effect of diosgenin on chondrocyte metabolisms in osteoarthritis].
abstract: To observe the effect of sirtuin type 1 (SIRT1) signaling pathway on chondrocyte metabolism and mitochondrial oxidative stress, to explore roles of SIRT1 signaling pathway and diosgenin (Dgn) in pathogenesis of osteoarthritis (OA).  Methods: To establish C57BL/6 mouse (13.5-18.0 g) model of OA. The chondrocytes were randomly assigned into 4 groups: an OA group, a Dgn+OA group, a Dgn+Sirtinol+OA group, and a Sirtinol+OA group. Expressions of SIRT1, acetylation-regulated transcription factor 1 (Ac-FOXO1), and Bax were detected in the 4 groups by Western blot. Changes in levels of succinate dehydrogenase, cytochrome C oxidase, and superoxide dismutase of mitochondrion in the 4 groups were observed.  Results: Compared with the OA group, SIRT1 protein expression was increased in the OA+Dgn group (P<0.05), while the Ac-FOXO1 and Bax protein expressions were decreased (P<0.05), the SDH and COX expressions were decreased, and the SOD content was decreased (P<0.05). Compared with the OA+Dgn group, the SIRT1 expression in the OA+Dgn+Sirtinol group and the Sirtinol+OA group was increased (P<0.05), while the Ac-FOXO1 and Bax protein expressions were decreased (P<0.05). The SDH and COX expressions were decreased, the SOD content was decreased, the SDH and COX expressions were increased, and the SOD content was increased in the OA group (All P<0.05), while those indexes were reversed in the OA+Dgn+Sirtinol group and the Sirtinol+OA group (All P<0.05).  Conclusion: The SIRT1 signaling pathway and OA are closely related to changes in chondrocyte apoptosis, in which Dgn may play a protective role in anti-OA by activating SIRT1 signaling pathway to inhibit apoptosis of chondrocytes and to increase chondrocyte mitochondrial oxidative stress capacity.
authors.affiliation: Department of Orthopaedics, General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Gerontology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiaole
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Orthopaedics, General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhen
authors.affiliation: Department of Orthopaedics, General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Shenghu
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedics, General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tian
authors.affiliation: Graduate School, Gansu University of Chinese Medicine, Lanzhou 730000, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, General Hospital of Lanzhou Command, Lanzhou 730050, China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001549:Benzamides; D019902:Chondrocytes / Q000378:metabolism*; D004144:Diosgenin / Q000494:pharmacology*; D003576:Electron Transport Complex IV / Q000378:metabolism; D000071161:Forkhead Box Protein O1 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009284:Naphthols; D010003:Osteoarthritis / Q000503:physiopathology*; D018384:Oxidative Stress / Q000502:physiology; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000502:physiology*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D051028:bcl-2-Associated X Protein
chemical_list: D001549:Benzamides; D000071161:Forkhead Box Protein O1; C467200:Foxo1 protein, mouse; D009284:Naphthols; D051028:bcl-2-Associated X Protein; C439060:sirtinol; D013482:Superoxide Dismutase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D004144:Diosgenin