title: Analysis of Yeast Mitochondria by Electron Microscopy.
abstract: Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method.
authors.affiliation: Institut für Zellbiologie, Universität Bayreuth, 95440, Bayreuth, Germany.
authors.forename: Ann-Katrin
authors.initials: AK
authors.lastname: Unger
authors.affiliation: Institut für Zellbiologie, Universität Bayreuth, 95440, Bayreuth, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Geimer
authors.affiliation: Max Planck Institut für Biochemie, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Harner
authors.affiliation: Max Planck Institut für Biochemie, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Walter
authors.initials: W
authors.lastname: Neupert
authors.affiliation: Institut für Zellbiologie, Universität Bayreuth, 95440, Bayreuth, Germany. benedikt.westermann@uni-bayreuth.de.
authors.forename: Benedikt
authors.initials: B
authors.lastname: Westermann
mesh_terms: D016616:Cryoultramicrotomy / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure; D057188:Workflow; D015003:Yeasts / Q000648:ultrastructure*
keywords: Immunoelectron microscopy; Mitochondria; Organelle architecture; Saccharomyces cerevisiae; Tokuyasu cryosectioning; Transmission electron microscopy