title: Microbiological and aMMP-8 findings depending on peri-implant disease in patients undergoing supportive implant therapy.
abstract: The aim of this study was to evaluate microbiological findings and aMMP-8 level of peri-implant mucositis (M) and peri-implantitis (P) in patients undergoing supportive implant therapy (SIT). Eighty-nine patients with 171 implants were included. The case definitions were as follows: M: PPD ≥4mm, BOP; P: PPD≥4mm, BOP, radiographic bone loss ≥3.5mm. Samples of peri-implant sulcular fluid (PISF) were taken from all peri-implant pockets at each implant to detect periodontal pathogens using PCR and aMMP-8 level with ELISA. Only Treponema denticola (Td) and Prevotella intermedia (Pi) showed significantly higher prevalence in P (healthy implants [HI]: Td=27%, Pi=17%; M: Td=26%, Pi=15%; P: Td and Pi=50%; P<0.05). The mean aMMP-8 level at implant sites did not show any significant difference (P=0.05) among HI (5.2±8.1), M (9.9±19.0), and P (4.9±7.7). Microbiological findings and aMMP-8 levels are not reliable criteria to distinguish between HI, M, and P in patients undergoing SIT.
authors.affiliation: Dept. of Cariology, Endodontology, and Periodontology, University Leipzig, Germany. Electronic address: dirk.ziebolz@medizin.uni-leipzig.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Ziebolz
authors.affiliation: Dept. of Cariology, Endodontology, and Periodontology, University Leipzig, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schmalz
authors.affiliation: Dept. of Preventive Dentistry, Periodontology and Cariology, University Medical Center Goettingen, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gollasch
authors.affiliation: Dept. of Periodontology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Eickholz
authors.affiliation: Dental Practice Hanau & Alzenau, Germany; Dept. of Prosthodontics, University Medical Center Goettingen, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Rinke
mesh_terms: D000328:Adult; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001826:Body Fluids / Q000737:chemistry / Q000382:microbiology; D003430:Cross-Sectional Studies; D015921:Dental Implants / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D008875:Middle Aged; D057873:Peri-Implantitis / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology*; D010518:Periodontitis / Q000628:therapy; D016133:Polymerase Chain Reaction; D013280:Stomatitis / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology*
chemical_list: D015921:Dental Implants; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8
keywords: Microbiological findings; Peri-implant mucositis; Peri-implantitis; aMMP-8