title: High expression of Aldolase A predicts poor survival in patients with clear-cell renal cell carcinoma.
abstract: BACKGROUND
  Aldolase A (ALDOA) is a glycolytic enzyme that drives the glycolytic metabolic pathway in mammalian cells. The overexpression of ALDOA was observed in a variety of cancers including clear-cell renal cell carcinoma (ccRCC). However, little was known about the clinicopathological significance and prognostic value of ALDOA in ccRCC patients.
  
  
  METHODS
  The expression of ALDOA was detected using immunohistochemical staining in 162 formalin-fixed, paraffin-embedded ccRCC sections. Prognostic outcomes correlated with ALDOA were examined using Kaplan-Meier analysis and the Cox proportional hazards model.
  
  
  RESULTS
  In patients with ccRCC, increased cytoplasmic ALDOA expression was positively associated with tumor size (P=0.021), TNM stages (P=0.034), lymph node metastasis (P=0.020), and overall survival (OS) (P<0.001). Kaplan-Meier analysis showed that high cytoplasmic expression of ALDOA was associated with a statistically significant lower OS (P<0.001). Multivariate analysis demonstrated that ALDOA expression was an independent and significant prognostic factor (HR =3.561, 95% CI =1.715-7.396,P=0.001). ALDOA expression was not associated with significant prognostic deference in the subgroups of TNM stage I patients or pT1 patients.
  
  
  CONCLUSIONS
  Our results suggest that ALDOA expression is an independent prognostic factor for OS in patients with ccRCC.
authors.affiliation: Department of Kidney Transplantation.
authors.forename: Ning
authors.initials: N
authors.lastname: Na
authors.affiliation: Department of Kidney Transplantation.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Genecology, The Third Affiliated Hospital of Sun Yat-sen University.
authors.forename: Chengfang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Kidney Transplantation.
authors.forename: Bin
authors.initials: B
authors.lastname: Miao
authors.affiliation: Department of Kidney Transplantation.
authors.forename: Liangqing
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Kidney Transplantation.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Organ Transplantation, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Jiang
keywords: Aldolase A; glycolysis; prognosis; renal carcinoma