title: Characterization and Complementation of a Chlorophyll-Less Dominant Mutant GL1 in Lagerstroemia indica.
abstract: Crape myrtle (Lagerstroemia indica) is a woody ornamental plant popularly grown because of its long-lasting, midsummer blooms and beautiful colors. The GL1 dominant mutant is the first chlorophyll-less mutant identified in crape myrtle. It was obtained from a natural yellow leaf bud mutation. We previously revealed that leaf color of the GL1 mutant is affected by light intensity. However, the mechanism of the GL1 mutant on light response remained unclear. The acclimation response of mutant and wild-type (WT) plants was assessed in a time series after transferring from low light (LL) to high light (HL) by analyzing chlorophyll synthesis precursor content, photosynthetic performance, and gene expression. In LL conditions, coproporphyrinogen III (Coprogen III) content had the greatest amount of accumulation in the mutant compared with WT, increasing by 100%. This suggested that the yellow leaf phenotype of the GL1 dominant mutant might be caused by disruption of coproporphyrinogen III oxidase (CPO) biosynthesis. Furthermore, the candidate gene, oxygen-independent CPO (HEMN), might only affect expression of upstream genes involved in chlorophyll metabolism in the mutant. Moreover, two genes, photosystem II (PSII) 10 kDa protein (psbR) and chlorophyll a/b binding protein gene (CAB1), had decreased mRNA levels in the GL1 mutant within the first 96 h following LL/HL transfer compared with the WT. Hierarchical clustering revealed that these two genes shared a similar expression trend as the oxygen-dependent CPO (HEMF). These findings provide evidence that GL1 is highly coordinated with PSII stability and chloroplast biogenesis.
authors.affiliation: 1 Southern Modern Forestry Collaborative Innovation Center, College of Biology and the Environment, Nanjing Forestry University , Nanjing, China .|2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Shu'an
authors.initials: S
authors.lastname: Wang
authors.affiliation: 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Lulu
authors.initials: L
authors.lastname: Gao
authors.affiliation: 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Rutong
authors.initials: R
authors.lastname: Yang
authors.affiliation: 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Linfang
authors.initials: L
authors.lastname: Li
authors.affiliation: 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Enliang
authors.initials: E
authors.lastname: Zhang
authors.affiliation: 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences, Nanjing, China .
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: 1 Southern Modern Forestry Collaborative Innovation Center, College of Biology and the Environment, Nanjing Forestry University , Nanjing, China .
authors.forename: Zengfang
authors.initials: Z
authors.lastname: Yin
mesh_terms: D000064:Acclimatization / Q000235:genetics; D002734:Chlorophyll / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D005799:Genes, Dominant; D005816:Genetic Complementation Test; D031563:Lagerstroemia / Q000235:genetics*; D008027:Light; D009154:Mutation; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified
chemical_list: D002734:Chlorophyll
keywords: antioxidant enzyme; chlorophyll b; high light; photosynthesis; qRT-PCR