title: Dysregulation of mRNA profile in cisplatin-resistant gastric cancer cell line SGC7901.
abstract: OBJECTIVE
  To explore novel therapeutic target of cisplatin resistance in human gastric cancer.
  
  
  METHODS
  The sensitivity of SGC7901 cells and cisplatin-resistant SGC7901 cells (SGC7901/DDP) for cisplatin were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. High-quality total RNA which isolated from SGC7901/DDP cells and SGC7901 cells were used for mRNA microarray analysis. Results were analyzed bioinformatically to predict their roles in the development of cisplatin resistance and the expression of 13 dysregulated mRNAs we selected were validated by quantitative real-time polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  SGC7901/DDP cells highly resistant to cisplatin demonstrated by MTT assay. A total of 1308 mRNAs (578 upregulated and 730 downregulated) were differentially expressed (fold change ≥ 2 andP-value < 0.05) in the SGC7901/DDP cells compared with SGC7901 cells. The expression of mRNAs detected by qRT-PCR were consistent with the microarray results. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway and protein-protein interaction analysis demonstrated that the differentially expressed mRNAs were enriched inPI3K-Akt,Notch,MAPK,ErbB,Jak-STAT,NF-kappaBsignaling pathways which may be involved in cisplatin resistance. Several genes such asPDE3B,VEGFC,IGFBP3,TLR4,HIPK2andEGFmay associated with drug resistance of gastric cancer cells to cisplatin.
  
  
  CONCLUSIONS
  Exploration of those altered mRNAs may provide more promising strategy in diagnosis and therapy for gastric cancer with cisplatin resistance.
authors.affiliation: Xiao-Que Xie, Hua Wang, Kang-Sheng Gu, Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Xiao-Que
authors.initials: XQ
authors.lastname: Xie
authors.affiliation: Xiao-Que Xie, Hua Wang, Kang-Sheng Gu, Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Qi-Hong
authors.initials: QH
authors.lastname: Zhao
authors.affiliation: Xiao-Que Xie, Hua Wang, Kang-Sheng Gu, Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Xiao-Que Xie, Hua Wang, Kang-Sheng Gu, Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Kang-Sheng
authors.initials: KS
authors.lastname: Gu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002945:Cisplatin / Q000494:pharmacology*; D019295:Computational Biology; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases; D002945:Cisplatin
keywords: Biology; Cisplatin resistance; Dysregulate; Gastric cancer; Microarray