title: H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers.
abstract: Normally, antibodies against influenza A have been prepared from viable virus or an engineered strain in certain hosts or cultured media. Two factors concerning antibody production are obvious. The obtaining antibody that is a kind of biomolecule has to be handled carefully, e.g., to be kept in a refrigerator. Furthermore, when the virus strain is highly pathogenic, such as H5N1, antibody production has to be done carefully in a high-level biosafety lab. Here, we show how to produce an antibody against H5N1 from a polymeric material using inactivated virus which can be conducted in a low-level biosafety lab. The process is based on imprinting the whole virus on a polymer surface to form molecularly imprinted polymers (MIPs). The MIPs show some properties of H5N1 antibody as they recognize H5N1 and have some important antibody activity. The H5N1 MIPs are not to be considered biomaterial, so they can be stored at room temperature and thus do not need any special care.
authors.affiliation: Faculty of Science, Department of Chemistry, Center for Advanced Studies in Nanotechnology and Its Applications in Chemical, Food and Agricultural Industries, Kasetsart University, 50 Ngam Wong Wan Rd., Chatuchak, Bangkok, 10900, Thailand. fscicsm@ku.ac.th.
authors.forename: Chak
authors.initials: C
authors.lastname: Sangma
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Vienna, Austria.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Lieberzeit
authors.affiliation: Faculty of Science, Department of Chemistry, Kasetsart University, Chatuchak, Bangkok, Thailand.
authors.forename: Wannisa
authors.initials: W
authors.lastname: Sukjee
mesh_terms: D000914:Antibodies, Viral / Q000737:chemistry / Q000378:metabolism*; D000917:Antibody Formation; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers; D013499:Surface Properties
chemical_list: D000914:Antibodies, Viral; D011108:Polymers
keywords: H5N1 MIPs; H5N1 influenza virus; Molecularly imprinted polymers; Plastic antibody; Surface imprinting; Synthetic H5N1 antibody