title: Purification of foot-and-mouth disease virus by heparin as ligand for certain strains.
abstract: The goal of this project was to develop an easily operable and scalable process for the recovery and purification of foot-and-mouth disease virus (FMDV) from cell culture. Heparin resins HipTrap Heparin HP and AF-Heparin HC-650 were utilized to purify FMDV O/HN/CHA/93. Results showed that the purity of AF-Heparin HC-650 was ideal. Then, the O/HN/CHA/93, O/Tibet/CHA/99, Asia I/HN/06, and A/CHA/HB/2009 strains were purified by AF-Heparin HC-650. Their affinity/virus recoveries were approximately 51.2%/45.8%, 71.5%/70.9%, 96.4%/73.5, and 59.5%/42.1%, respectively. During a stepwise elution strategy, the viral particles were mainly eluted at 300mM ionic strength peaks. The heparin affinity chromatography process removed more than 94% of cellular and medium proteins. Anion exchange resin Capto Q captured four FMD virus particles; 40% of binding proteins and 80%-90% of viral particles were eluted at 450mM NaCl. Moreover, ionic strength varied from 30 to 450mM had no effect on the immunity to FMDV. The results revealed that heparin sulfate may be the main receptor for CHA/99 strain attachment-susceptible cells. Heparin affinity chromatography can reach perfect results, especially when used as a ligand of the virus. Anion exchange is useful only as previous step for further purification.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: westernboydp555@sohu.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Du
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: shiqisun21@hotmail.com.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: Dzhxia@126.com.
authors.forename: Jinjie
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: zhixiaoying333@163.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhi
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: cyanyan_820116@163.com.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: 845698073@qq.com.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Teng
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: guohuichen@caas.cn.
authors.forename: Huichen
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: liuzaixin@caas.cn.
authors.forename: Zaixin
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods*; D006224:Cricetinae; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D014776:Virus Cultivation
chemical_list: D008024:Ligands; D006493:Heparin
keywords: Anion exchange; Foot-and-mouth disease virus; Heparin affinity chromatography; Virus purification