title: HSV-1 ICP27 induces apoptosis by promoting Bax translocation to mitochondria through interacting with 14-3-3θ.
abstract: The subcellular localization of Bax plays a crucial role during apoptosis. In response to apoptotic stimuli, Bax translocates from the cytoplasm to the mitochondria, where it promotes the release of cytochrome c to the cytoplasm. In cells infected with HSV-1, apoptosis is triggered or blocked by diverse mechanisms. In this study, we demonstrate how HSV-1 ICP27 induces apoptosis and modulates mitochondrial membrane potential in HEK 293T cells. We found that ICP27 interacts with 14-3-3θ which sequesters Bax to the cytoplasm. In addition, ICP27 promotes the translocation of Bax to the mitochondria by inhibiting the interaction between 14-3-3θ and Bax. Our findings may provide a novel apoptotic regulatory pathway induced by ICP27 during HSV-1 infection. [BMB Reports 2017; 50(5): 257-262].
authors.affiliation: Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Ji Ae
authors.initials: JA
authors.lastname: Kim
authors.affiliation: Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Jin Chul
authors.initials: JC
authors.lastname: Kim
authors.affiliation: Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Min
authors.affiliation: Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Inho
authors.initials: I
authors.lastname: Kang
authors.affiliation: Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Jeongho
authors.initials: J
authors.lastname: Oh
authors.affiliation: Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Jeong Keun
authors.initials: JK
authors.lastname: Ahn
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D003593:Cytoplasm; D057809:HEK293 Cells; D018259:Herpesvirus 1, Human; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D008928:Mitochondria; D000069284:Protein Translocation Systems / Q000378:metabolism / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; D002352:Carrier Proteins; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D000069284:Protein Translocation Systems; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D020169:Caspases