title: In Situ Monitoring Intracellular Structural Change of Nanovehicles through Photoacoustic Signals Based on Phenylboronate-Linked RGD-Dextran/Purpurin 18 Conjugates.
abstract: The stimuli-responsive polymeric nanocarriers have been studied extensively, and their structural changes in cells are important for the controlled intracellular drug release. The present work reported RGD-dextran/purpurin 18 conjugates with pH-responsive phenylboronate as spacer for monitoring the structural change of nanovehicles through ratiometric photoacoustic (PA) signal. Phenylboronic acid modified purpurin 18 (NPBA-P18) could attach onto the RGD-decorated dextran (RGD-Dex), and the resulting RGD-Dex/NPBA-P18 (RDNP) conjugates with different molar ratios of RGD-Dex and NPBA-P18 were prepared. When the moles of NPBA-P18 were equivalent to more than triple of RGD-Dex, the single-stranded RDNP conjugates could self-assemble into nanoparticles in aqueous solution due to the fairly strong hydrophobicity of NPBA-P18. The pH-responsive aggregations of NPBA-P18 were investigated by UV-vis, fluorescence, and circular dichroism spectra, as well as transmission electron microscope. Based on distinct PA signals between monomeric and aggregated state, ratiometric PA signal of I750/I710could be presented to trace the structural change progress. Compared with RDNP single chains, the nanoparticles exhibited effective cellular internalization through endocytosis pathway. Furthermore, the nanoparticles could form well-ordered aggregates responding to intracellular acidic environment, and the resulting structural change was also monitored by ratiometric PA signal. Therefore, the noninvasive PA approach could provide a deep insight into monitoring the intracellular structural change process of stimuli-responsive nanocarriers.
authors.affiliation: CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, China.
authors.forename: Dong-Bing
authors.initials: DB
authors.lastname: Cheng
authors.affiliation: CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, China.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Qi
authors.affiliation: CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, China.
authors.forename: Jing-Qi
authors.initials: JQ
authors.lastname: Wang
authors.affiliation: CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cong
authors.affiliation: CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, China.
authors.forename: Fu-Hua
authors.initials: FH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Drug Research and Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences , 501 Haike Road, Shanghai 201203, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Yu
authors.affiliation: CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, China.
authors.forename: Zeng-Ying
authors.initials: ZY
authors.lastname: Qiao
authors.affiliation: CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, China.
authors.forename: Hao
authors.identifier: 0000-0002-1961-0787
authors.initials: H
authors.lastname: Wang
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D061088:Photoacoustic Techniques; D011108:Polymers / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D003911:Dextrans; D004337:Drug Carriers; D009842:Oligopeptides; D011108:Polymers; D011166:Porphyrins; C059051:purpurin 18; C047981:arginyl-glycyl-aspartic acid; C010686:benzeneboronic acid