title: The local administration of parathyroid hormone encourages the healing of bone defects in the rat calvaria: Micro-computed tomography, histological and histomorphometric evaluation.
abstract: OBJECTIVE
  To evaluate the effect of a single-dose local administration of PTH on bone healing in rat calvarial bone defects by means of micro-computed tomography, histological and histomorphometric analysis.
  
  
  METHODS
  Critical-size cranial osteotomy defects were created in 42 male rats. The animals were randomly divided into 3 groups. In the C Group, the bone defect was only filled with a blood clot. In the S Group, it was filled with a collagen sponge and covered with bovine cortical membrane. In the PTH Group, the defect was filled with a collagen sponge soaked with PTH and covered with bovine cortical membrane. The groups were further split in two for euthanasia 15 and 60days post-surgery. Data was statistically analyzed with t-tests for independent samples or the nonparametric Mann-Whitney test when applicable. Intragroup comparisons were analyzed with paired t-tests (p<0.05).
  
  
  RESULTS
  Micro-CT analysis results did not demonstrate statistically significant intergroup differences. At 15days post-surgery, the histomorphometric analysis showed that the PTH Group exhibited a significantly higher percentage of bone formation compared with the S Group. At 60days post-surgery, a higher percentage of new bone was observed in the PTH group.
  
  
  CONCLUSIONS
  The results suggest that the local administration of PTH encouraged the bone healing in critical-size calvarial defects in rats.
authors.affiliation: Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil.
authors.forename: Caroline Moreira
authors.initials: CM
authors.lastname: Auersvald
authors.affiliation: Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil.
authors.forename: Felipe Rychuv
authors.initials: FR
authors.lastname: Santos
authors.affiliation: Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil.
authors.forename: Mayara Mytie
authors.initials: MM
authors.lastname: Nakano
authors.affiliation: Departamento de Odontologia Restauradora, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/no - Campus da USP Ribeirão Preto, SP, CEP 14040-904, Brazil.
authors.forename: Graziela Bianchi
authors.initials: GB
authors.lastname: Leoni
authors.affiliation: Departamento de Odontologia Restauradora, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/no - Campus da USP Ribeirão Preto, SP, CEP 14040-904, Brazil.
authors.forename: Manoel Damião
authors.initials: MD
authors.lastname: de Sousa Neto
authors.affiliation: Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil.
authors.forename: Rafaela
authors.initials: R
authors.lastname: Scariot
authors.affiliation: Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil.
authors.forename: Allan Fernando
authors.initials: AF
authors.lastname: Giovanini
authors.affiliation: Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil. Electronic address: tdeliberador@gmail.com.
authors.forename: Tatiana Miranda
authors.initials: TM
authors.lastname: Deliberador
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D002417:Cattle; D003094:Collagen / Q000494:pharmacology; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology* / Q000601:surgery; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D010281:Parathyroid Hormone; D003094:Collagen
keywords: Bone regeneration; Calvaria; Micro-CT; Parathyroid hormone; Rats