title: Effect of yeasts on biodegradation potential of immobilized cultures of white rot fungi.
abstract: The aim was to investigate the effect of yeast organisms on the degradation process by immobilized cultures of ligninolytic fungi. Immobilization was accomplished by 7-day colonization of polyamide mesh with mycelial fragments. Irpex lacteus decolorized >90% of the initial concentration of 150mgl-1of anthraquinone Remazol Brilliant Blue R dye in three subsequent decolorization cycles and the degradation capacity was not negatively affected by the presence of 106Saccharomyces cerevisiae cells per ml in the mixed culture. The yeast was not able to degrade the dye. I. lacteus biofilm was also resistant to bacterial infection with E. coli. Inoculation of the yeast to pre-formed I. lacteus biofilm culture resulted in a reduction of fungal biomass by 27%. Levels of LiP, MnP and laccase of I. lacteus were not much influenced by S. cerevisiae or E. coli. Similar resilience of P. ostreatus biofilms was observed after exposure to yeast Issatchenkia occidentalis when the fungal degradation capacity measured with Reactive Orange 16 azo dye was maintained over two decolorization cycles. I. occidentalis did not degrade the dye under the conditions used. Formation of densely packed fungal biofilms with abundant extracellular polysaccharide was not impeded by the yeast. Increase of MnP and laccase levels attributable to the presence of I. occidentalis was observed.
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic. Electronic address: Pavlina.Slosarcikova@email.cz.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Šlosarčíková
authors.affiliation: Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic. Electronic address: novotny@biomed.cas.cz.
authors.forename: Čeněk
authors.initials: Č
authors.lastname: Novotný
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic. Electronic address: Katerina.Malachova@osu.cz.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Malachová
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic. Electronic address: hana.valkova1@email.cz.
authors.forename: Hana
authors.initials: H
authors.lastname: Válková
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic. Electronic address: jindrich.fojtik@seznam.cz.
authors.forename: Jindřich
authors.initials: J
authors.lastname: Fojtík
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D004926:Escherichia coli; D042845:Laccase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D012441:Saccharomyces cerevisiae
chemical_list: D004396:Coloring Agents; D042845:Laccase; D010544:Peroxidases
keywords: Biodegradation; Fungal biofilm; Mixed culture; Synthetic dyes; Yeasts