title: Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus.
abstract: Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330.8 kDa) in the vc strand. Analyses of the NSm, Gn/Gc and RdRp of PCSV revealed that PCSV is phylogenetically clustered within the watermelon silver mottle virus-related clade. Based on the whole genome sequence, PCSV is closely related to Tomato necrotic ringspot virus and should be classified as a new tospovirus species.
authors.affiliation: Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan.|Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Zhongzheng Dist., Taipei, 10070, Taiwan.
authors.forename: Kuo-Shiou
authors.initials: KS
authors.lastname: Huang
authors.affiliation: Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Chia-Hsing
authors.initials: CH
authors.lastname: Tai
authors.affiliation: Plant Pathology Division, Taiwan Agricultural Research Institute, Wufeng, Taichung, 41362, Taiwan.
authors.forename: Ying-Huey
authors.initials: YH
authors.lastname: Cheng
authors.affiliation: Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Lin
authors.affiliation: Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan. kikichenwolf@hotmail.com.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan. kikichenwolf@hotmail.com.
authors.forename: Tsung-Chi
authors.initials: TC
authors.lastname: Chen
authors.affiliation: Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan. fjjan@nchu.edu.tw.
authors.forename: Fuh-Jyh
authors.initials: FJ
authors.lastname: Jan
mesh_terms: D000595:Amino Acid Sequence; D018551:Solanum lycopersicum / Q000821:virology; D010802:Phylogeny; D029222:Piper nigrum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D013624:Taiwan; D017865:Tospovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase