title: Hepatotoxicity of Eupatorium adenophorum extracts and the identification of major hepatotoxic components.
abstract: Eupatorium adenophorum can induce liver toxicity in animals. For the safe utilisation of the weed, the hepatotoxic components need to be discovered. In this study, in vitro hepatotoxicity of different extracts from E. adenophorum were determined on human hepatocyte cell line L02 and hepatocellular carcinoma cell line HepG2. The results showed that water extracts of E. adenophorum exhibited no hepatotoxicity in vitro while high concentrations of the organic solvent extracts had obvious hepatotoxicity. Sesquiterpenes may contribute to the toxicity based on the comparison of composition analysis. Three cadinene sesquiterpenes were purified and identified as 9-oxo-10,11-dehydroageraphorone, 10Hα-9-oxo-ageraphorone and 10Hβ-9-oxo-ageraphorone. In vitro hepatotoxic effects of these components were investigated, the IC50of the three compounds were 122.53, 87.52, and 108.80 μM in L02 cells and 151.92, 104.48, and 138.08 μM in HepG2 cells by Cell Counting Kit-8 (CCK-8) assay. The three components were confirmed to be, at least partial, hepatotoxic components.
authors.affiliation: a Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering , Institute of Process Engineering, Chinese Academy of Sciences , Beijing , PR China.|b University of Chinese Academy of Sciences , Beijing , PR China.
authors.forename: Boyan
authors.initials: B
authors.lastname: Liu
authors.affiliation: a Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering , Institute of Process Engineering, Chinese Academy of Sciences , Beijing , PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Cao
authors.affiliation: a Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering , Institute of Process Engineering, Chinese Academy of Sciences , Beijing , PR China.|b University of Chinese Academy of Sciences , Beijing , PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering , Institute of Process Engineering, Chinese Academy of Sciences , Beijing , PR China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Yuan
authors.affiliation: a Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering , Institute of Process Engineering, Chinese Academy of Sciences , Beijing , PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D036603:Ageratina / Q000737:chemistry*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology*; D056945:Hep G2 Cells; D006801:Humans; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D012717:Sesquiterpenes / Q000737:chemistry / Q000633:toxicity; D018675:Toxicity Tests
chemical_list: D010936:Plant Extracts; D012717:Sesquiterpenes
keywords: Eupatorium adenophorum; different solvent extracts; hepatotoxicity; sesquiterpenes