title: Differentiation of protein secondary structure in clear and opaque human lenses: AFM - IR studies.
abstract: Here, we present the first approach to human lenses investigations with and without cataract development changes in nanoscale resolution using AFM - IR spectroscopy. We proved that the application of this technique allowed us to better understand of structural changes connected with advancing disease process in studied lenses. The obtained results show the impact of the disease development on the secondary structure of proteins in these human tissues. The domination of the β-turn protein secondary structure is observed in the clear (non-affected by cataract) lens. While, in the case of the opaque (cataractous) samples the different degree of the degradation due to development of cataract, was recognized. Briefly, this process is associated with the protein secondary changes from β-turn/β-sheet parallel for less altered part of the lens to stable anti-parallel β-sheet for the more degraded part. Interestingly, the AFM - IR technique provided estimation of the protein secondary structure without the need for using deconvolution procedure.
authors.affiliation: Institute of Nuclear Physics Polish Academy of Sciences, PL-31342 Krakow, Poland.
authors.forename: C
authors.initials: C
authors.lastname: Paluszkiewicz
authors.affiliation: Institute of Nuclear Physics Polish Academy of Sciences, PL-31342 Krakow, Poland. Electronic address: natalia.piergies@ifj.edu.pl.
authors.forename: N
authors.initials: N
authors.lastname: Piergies
authors.affiliation: 5th Military Hospital with Polyclinic in Krakow, Ophthalmology, PL-30091 Krakow, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Chaniecki
authors.affiliation: Military Medical Institute, Ophthalmology, PL-04141 Warszawa, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Rękas
authors.affiliation: 5th Military Hospital with Polyclinic in Krakow, Ophthalmology, PL-30091 Krakow, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Miszczyk
authors.affiliation: Institute of Nuclear Physics Polish Academy of Sciences, PL-31342 Krakow, Poland.
authors.forename: W M
authors.initials: WM
authors.lastname: Kwiatek
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry*; D008297:Male; D018625:Microscopy, Atomic Force / Q000379:methods*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
keywords: Cataract; Human lenses; Infrared spectroscopy with atomic force microscope (AFM – IR)