title: Correlation study on chromogranin A genetic polymorphism and prognosis of critically ill patients.
abstract: The objective was to investigate the correlation between single nucleotide polymorphism (SNP) of chromogranin A (CHGA) and prognosis of critically ill patients.
  
  
  
  We screened 357 critically ill patients consecutively admitted to our intensive care unit. The -89/-415/-462 SNP locus in the promoter region and the +9559/+9578/+9590/+9611 SNP locus in exon 7 coding of CHGA were genotyped by polymerase chain reaction and DNA sequencing technology. Subsequently, the correlation between genotype and prognosis of patients was analyzed.
  
  
  
  (1) Three hundred critically ill Chinese Han patients were enrolled in the study. CHGA-415/-462/+9559/+9611 SNPs were polymorphically distributed. Phenotypes of the 4 SNPs were shown not to be in linkage disequilibrium, and there were no significant differences in the minor allele frequencies (MAFs) of the 4 SNPs between participants of this study and healthy people in Asia. (2) The CHGA-415 T/C MAF of the nonsurvival group was significantly higher than that of the survival group (MAF 0.3813 and 0.2864, respectively; P=.026). Survival analysis showed that there were significant differences between the CHGA-415 T/C mutation group (including TC and CC genotypes) and the wild-type group (TT genotype) (log rank=8.887, P=.003). The mortality in the mutant group was significantly higher than that in the wild-type group (0.3333 and 0.1852, respectively; P=.004). (3) Binary logistic analysis showed that CHGA-415 T/C polymorphism was an independent risk factor for the mortality of critically ill patients (odds ratio, 2.286; 95% confidence interval, 1.165-4.484; P=.016).
  
  
  
  Critically ill patients with CHGA-415 T/C mutant genotype display higher 30-day mortality than those with the wild-type group. CHGA-415 T/C polymorphism is an independent risk factor of poor prognosis in critically ill Chinese Han patients.
authors.affiliation: Department of Emergency Medicine and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jing-Lun
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Department of Emergency Medicine and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Ni-Na
authors.initials: NN
authors.lastname: Gu
authors.affiliation: Department of Central Laboratory, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jin-Ye
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Department of Emergency Medicine and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Jiang
authors.affiliation: Department of Emergency Medicine and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. Electronic address: doctor_zhangdan@126.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Endocrine and Breast Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. Electronic address: doctorrenguosheng@126.com.
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Ren
mesh_terms: D000328:Adult; D000368:Aged; D002681:China / Q000208:ethnology; D053379:Chromogranin A / Q000235:genetics*; D016638:Critical Illness; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors
chemical_list: C506150:CHGA protein, human; D053379:Chromogranin A
keywords: Chromogranin A; Critically ill patients; Minor allele frequency; Prognosis; Single nucleotide polymorphism