title: Large-scale human skin lipidomics by quantitative, high-throughput shotgun mass spectrometry.
abstract: The lipid composition of human skin is essential for its function; however the simultaneous quantification of a wide range of stratum corneum (SC) and sebaceous lipids is not trivial. We developed and validated a quantitative high-throughput shotgun mass spectrometry-based platform for lipid analysis of tape-stripped SC skin samples. It features coverage of 16 lipid classes; total quantification to the level of individual lipid molecules; high reproducibility and high-throughput capabilities. With this method we conducted a large lipidomic survey of 268 human SC samples, where we investigated the relationship between sampling depth and lipid composition, lipidome variability in samples from 14 different sampling sites on the human body and finally, we assessed the impact of age and sex on lipidome variability in 104 healthy subjects. We found sebaceous lipids to constitute an abundant component of the SC lipidome as they diffuse into the topmost SC layers forming a gradient. Lipidomic variability with respect to sampling depth, site and subject is considerable, and mainly accredited to sebaceous lipids, while stratum corneum lipids vary less. This stresses the importance of sampling design and the role of sebaceous lipids in skin studies.
authors.affiliation: Medical Faculty, Dresden University of Technology, Dresden, Germany.|Lipotype GmbH, Dresden, Germany.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sadowski
authors.affiliation: Lipotype GmbH, Dresden, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Klose
authors.affiliation: Lipotype GmbH, Dresden, Germany.
authors.forename: Mathias J
authors.initials: MJ
authors.lastname: Gerl
authors.affiliation: Department of Dermatology, Venerology and Allergology, University of Medicine, Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wójcik-Maciejewicz
authors.affiliation: Lipotype GmbH, Dresden, Germany.
authors.forename: Ronny
authors.initials: R
authors.lastname: Herzog
authors.affiliation: Lipotype GmbH, Dresden, Germany.|Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Simons
authors.affiliation: Department of Dermatology, Venerology and Allergology, University of Medicine, Wroclaw, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Reich
authors.affiliation: Lipotype GmbH, Dresden, Germany.
authors.forename: Michal A
authors.initials: MA
authors.lastname: Surma
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D008055:Lipids