title: Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus.
abstract: To characterize neutralizing mimotopes, phages were selected from a 12-mer phage display library using three anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibodies: (1) A1; (2) A2; and (3) A7. Of these, A2 and A7 recognize the mimotope, P2, which contains the SRHDHIH motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the N-terminal ectodomain of GP3. The artificial multi-epitope gene, mp2, was designed by combining three repeats of the mimotope P2. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp2). The rSPV-mp2 was able to stably express the multi-epitope peptide, mP2, in vitro. The rSPV-mp2 immunized pigs exhibited a significantly shorter fever duration compared with the wtSPV treated group (P < 0.05). There was an enhanced humoral and cellular immune response, decreased number of PRRSV genomic copies, and a significant reduction in the gross lung pathology (P < 0.05) was observed following PRRSV infection in rSPV-mp2-immunized animals. The results suggest that the recombinant rSPV-mp2 provided pigs with significant protection against PRRSV infection.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Huixing
authors.initials: H
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hou
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Fan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature; D000939:Epitopes / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008168:Lung / Q000473:pathology; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012367:RNA, Viral / Q000097:blood; D018148:Suipoxvirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013552:Swine; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000939:Epitopes; D019151:Peptide Library; D010455:Peptides; D012367:RNA, Viral; D014765:Viral Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma