title: Cutaneous leishmaniasis in French Guiana: revising epidemiology with PCR-RFLP.
abstract: BACKGROUND
  The development of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in French Guiana.
  
  
  METHODS
  A descriptive study of theLeishmaniaspecies was identified, and their spatiotemporal distribution was conducted using patient records between 2006 and 2013, with 1017 new cases of leishmaniasis diagnosed. Identification was realized by PCR-RFLP on 745 cases.
  
  
  RESULTS
  The average proportions for different species were 86.2% forLeishmania (Vianna) guyanensis; 9.7% forLeishmania (Vianna) braziliensis; 2.8% forLeishmania (Leishmania) amazonensis; and 1.3% forLeishmania (Vianna) lainsoni, and no case ofLeishmania (Vianna) naiffiwas identified. Over this period, the proportion of cases due toL. (V.) braziliensisseemed to increase from 8.9% in 2006 to 13.0% in 2013 notably near the gold mining zones.
  
  
  CONCLUSIONS
  The use of molecular tools has transformed the view of the local epidemiology of cutaneous leishmaniasis in French Guiana.
authors.affiliation: Equipe EA3593 Ecosystemes Amazoniens et Pathologie Tropicale, Université de la Guyane, Campus Saint Denis, Avenue d'Estrées, 97300 Cayenne, French Guiana.|Laboratoire Associé - Centre National de Référence Leishmania, Laboratoire Hospito-Universitaire de Parasitologie Mycologie, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Stephane
authors.initials: S
authors.lastname: Simon
authors.affiliation: Equipe EA3593 Ecosystemes Amazoniens et Pathologie Tropicale, Université de la Guyane, Campus Saint Denis, Avenue d'Estrées, 97300 Cayenne, French Guiana.|Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane CIC CIE 1424, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Nacher
authors.affiliation: Equipe EA3593 Ecosystemes Amazoniens et Pathologie Tropicale, Université de la Guyane, Campus Saint Denis, Avenue d'Estrées, 97300 Cayenne, French Guiana.|Laboratoire Associé - Centre National de Référence Leishmania, Laboratoire Hospito-Universitaire de Parasitologie Mycologie, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Bernard
authors.initials: B
authors.lastname: Carme
authors.affiliation: Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane CIC CIE 1424, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Celia
authors.initials: C
authors.lastname: Basurko
authors.affiliation: Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane CIC CIE 1424, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Amaury
authors.initials: A
authors.lastname: Roger
authors.affiliation: Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane CIC CIE 1424, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Antoine
authors.initials: A
authors.lastname: Adenis
authors.affiliation: Equipe EA3593 Ecosystemes Amazoniens et Pathologie Tropicale, Université de la Guyane, Campus Saint Denis, Avenue d'Estrées, 97300 Cayenne, French Guiana.|Laboratoire Associé - Centre National de Référence Leishmania, Laboratoire Hospito-Universitaire de Parasitologie Mycologie, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Marine
authors.identifier: 0000-0003-2331-6588
authors.initials: M
authors.lastname: Ginouves
authors.affiliation: Equipe EA3593 Ecosystemes Amazoniens et Pathologie Tropicale, Université de la Guyane, Campus Saint Denis, Avenue d'Estrées, 97300 Cayenne, French Guiana.|Laboratoire Associé - Centre National de Référence Leishmania, Laboratoire Hospito-Universitaire de Parasitologie Mycologie, Cayenne General Hospital, 97300 Cayenne, French Guiana.
authors.forename: Magalie
authors.initials: M
authors.lastname: Demar
authors.affiliation: Equipe EA3593 Ecosystemes Amazoniens et Pathologie Tropicale, Université de la Guyane, Campus Saint Denis, Avenue d'Estrées, 97300 Cayenne, French Guiana.|Laboratoire Associé - Centre National de Référence Leishmania, Laboratoire Hospito-Universitaire de Parasitologie Mycologie, Cayenne General Hospital, 97300 Cayenne, French Guiana.|Department of Dermatology, Cayenne General Hospital, 97300 Cayenne, French Guiana France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Couppie
keywords: Cutaneous leishmaniasis; Epidemiology; French Guiana; Leishmania braziliensis; PCR-RFLP