title: Effect of ultrasound assisted extraction on the physicochemical and functional properties of collagen from soft-shelled turtle calipash.
abstract: The aim of the present study was to evaluate the physicochemical and functional properties of acid-soluble collagen by ultrasound assisted extraction (UASC) from calipash of soft-shelled turtle (Pelodiscus sinensis). The results showed the collagen content was increased by 16.3% in UASC over the collagen from the conventional extraction (ASC). Both ASC and UASC contained a moderate amount of imino acid (197 and 216 residues/1000 residues, respectively) and hydrophobic amino acid (353 and 391 residues/1000 residues, respectively) in amino acid composition. Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analyses confirmed that the ultrasound treatment did not disrupt the triple-stranded helical structures in UASC. UASC had higher thermal stability compared with ASC by viscosity and differential scanning calorimetry (DSC) measurements, therefore, UASC might have the advantage to be used. In dynamic elastic behavior measurement, UASC showed a larger elasticity than ASC. With a mild modification by ultrasound, UASC had superior functional properties to ASC, including water/oil absorption capacity, water-holding capacity, emulsifying properties and foaming properties. These results suggested that UASC from the soft-shelled turtle calipash had a potential to be used widely in food, medicine, cosmetics and biomedical materials.
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.affiliation: College of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Ginling College, Nanjing Normal University, Nanjing, 210024, PR China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Cai
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
authors.forename: Muhan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
authors.forename: Zhilan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Sun
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China. Electronic address: weiminxu2002@aliyun.com.
authors.forename: Weimin
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China; Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: daoyingwang@yahoo.com.
authors.forename: Daoying
authors.initials: D
authors.lastname: Wang
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D060105:Animal Shells; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D055598:Chemical Phenomena; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification*; D011489:Protein Denaturation; D012212:Rheology; D012995:Solubility; D014426:Turtles; D000069453:Ultrasonic Waves; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D003094:Collagen; D019342:Acetic Acid
keywords: Physicochemical properties; Soft-shelled turtle calipash collagen; Ultrasound assisted extraction