title: Paracrine influence of human perivascular cells on the proliferation of adenocarcinoma alveolar epithelial cells.
abstract: Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-1α and K562)in vitrousing Transwell® co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-1α and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Eunbi
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Obstetrics & Gynecology, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Sunghun
authors.initials: S
authors.lastname: Na
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Borim
authors.initials: B
authors.lastname: An
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Se-Ran
authors.initials: SR
authors.lastname: Yang
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Kwon-Soo
authors.initials: KS
authors.lastname: Ha
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Eun-Taek
authors.initials: ET
authors.lastname: Han
authors.affiliation: Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.affiliation: Department of Molecular Microbiology and Immunology, Department of Medicine, Alpert Medical School, Brown University, Providence, Rhode Island 02912, US.
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lee
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Sanji University, Wonju 26339, Korea.
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Seung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Hong
keywords: Cancer; Paracrine effect; Perivascular cells; Proliferation