title: Mosquito-Borne Diseases and Omics: Salivary Gland Proteome of the Female Aedes aegypti Mosquito.
abstract: The female Aedes aegypti mosquito is an important vector for several tropical and subtropical diseases such as dengue, chikungunya, and Zika and yellow fever. The disease viruses infect the mosquito and subsequently spread to the salivary glands after which the viruses can be transmitted to humans with probing or feeding by the mosquito. Omics systems sciences offer the opportunity to characterize vectors and can inform disease surveillance, vector control and development of innovative diagnostics, personalized medicines, vaccines, and insecticide targets. Using high-resolution mass spectrometry, we performed an analysis of the A. aegypti salivary gland proteome. The A. aegypti proteome resulted in acquisition of 83,836 spectra. Upon searches against the protein database of the A. aegypti, these spectra were assigned to 5417 unique peptides, belonging to 1208 proteins. To the best of our knowledge, this is the largest set of proteins identified in the A. aegypti salivary gland. Of note, 29 proteins were involved in immunity-related pathways in salivary glands. A subset of these proteins is known to interact with disease viruses. Another 15 proteins with signal cleavage site were found to be secretory in nature, and thus possibly playing critical roles in blood meal ingestion. These findings provide a baseline to advance our understanding of vector-borne diseases and vector-pathogen interactions before virus transmission in global health, and might therefore enable future design and development of virus-blocking strategies and novel molecular targets in the mosquito vector A. aegypti.
authors.affiliation: 1 Department of Community Medicine, Armed Forces Medical College , Pune, India .|2 National Institute of Malaria Research , Goa, India .|3 Department of Zoology, Goa University , Goa, India .
authors.forename: Rakhi
authors.initials: R
authors.lastname: Dhawan
authors.affiliation: 4 Institute of Bioinformatics , International Technology Park, Bangalore, India .|5 Manipal University , Manipal, India .
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.affiliation: 2 National Institute of Malaria Research , Goa, India .
authors.forename: Ajeet Kumar
authors.initials: AK
authors.lastname: Mohanty
authors.affiliation: 4 Institute of Bioinformatics , International Technology Park, Bangalore, India .|5 Manipal University , Manipal, India .
authors.forename: Gourav
authors.initials: G
authors.lastname: Dey
authors.affiliation: 4 Institute of Bioinformatics , International Technology Park, Bangalore, India .|5 Manipal University , Manipal, India .
authors.forename: Jayshree
authors.initials: J
authors.lastname: Advani
authors.affiliation: 4 Institute of Bioinformatics , International Technology Park, Bangalore, India .|6 YU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University , Mangalore, India .|7 NIMHANS-IOB Proteomics and Bioinformatics Laboratory, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences , Bangalore, India .
authors.forename: T S Keshava
authors.initials: TS
authors.lastname: Prasad
authors.affiliation: 2 National Institute of Malaria Research , Goa, India .
authors.forename: Ashwani
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000330:Aedes / Q000378:metabolism*; D000818:Animals; D005260:Female; D019476:Insect Proteins / Q000378:metabolism*; D057285:Precision Medicine; D040901:Proteomics / Q000379:methods*; D012469:Salivary Glands / Q000378:metabolism*
chemical_list: D019476:Insect Proteins
keywords: diagnostic medicine; global health; neglected tropical diseases; personalized medicine; proteomics