title: Effects of a non-thermal plasma needle device on HPV-16 positive cervical cancer cell viability in vitro.
abstract: Non-thermal atmospheric pressure plasma has been demonstrated to inactivate a wide range of surface-dwelling pathogenic microorganisms including airborne viral particles, vegetative bacteria and bacterial spores. This shows the promise of plasma-based decontamination procedures for broad-spectrum sterilization and disinfection and promotes the in-depth and systematic study of how plasma treatment conditions relate to pathogen inactivation efficiency. A wide knowledge gap nonetheless exists regarding whether certain plasma parameters and exposure conditions can alter the resistance of virus-linked cancer cells to treatment. The current work reveals the effects of a non-thermal needle-shaped atmospheric-pressure plasma on the viability of an adherent human cervical carcinoma cell line containing a human papillomavirus type 16 (HPV-16) provirus. Using a helium plasma device driven by 8 kV, 2 kHz, 150 ns pulses, CaSki cells moistened with culture medium were exposed to plasma for different treatment durations, gap distances and gas flow rates. Open-well exposure to helium flow alone for 120 s did not produce significant changes in CaSki cell viability. By comparison, cells exposed to plasma showed a dose-dependent reduction in viability from at least 15% to 60% compared to the control. These findings reveal possibilities for NTP treatment of HPV-16 infected cervical cancers and indicate the importance of NTP parameters to treatment outcome.
authors.forename: Hollie A
authors.initials: HA
authors.lastname: Ryan
authors.forename: Johanna
authors.initials: J
authors.lastname: Neuber
authors.lastname: Shutong Song
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Beebe
authors.lastname: Chunqi Jiang
mesh_terms: D002470:Cell Survival; D005260:Female; D052162:Human papillomavirus 16; D006801:Humans; D009339:Needles; D058626:Plasma Gases / Q000494:pharmacology*; D013242:Sterilization; D002583:Uterine Cervical Neoplasms / Q000628:therapy*
chemical_list: D058626:Plasma Gases