title: Enhanced Performance of Colorimetric Biosensing on Paper Microfluidic Platforms Through Chemical Modification and Incorporation of Nanoparticles.
abstract: This chapter describes two different methodologies used to improve the analytical performance of colorimetric paper-based biosensors. Microfluidic paper-based analytical devices (μPADs) have been produced by a stamping process and CO2laser ablation and modified, respectively, through an oxidation step and incorporation of silica nanoparticles on the paper structure. Both methods are employed in order to overcome the largest problem associated with colorimetric detection, the heterogeneity of the color distribution in the detection zones. The modification steps are necessary to improve the interaction between the paper surface and the selected enzymes. The enhanced performance has ensured reliability for quantitative analysis of clinically relevant compounds.
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil.
authors.forename: Ellen Flávia Moreira
authors.initials: EF
authors.lastname: Gabriel
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil.
authors.forename: Paulo T
authors.initials: PT
authors.lastname: Garcia
authors.affiliation: Department of Chemistry, Clemson University, Clemson, SC 29634, TX, 78249, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Evans
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil.
authors.forename: Thiago M G
authors.initials: TM
authors.lastname: Cardoso
authors.affiliation: Department of Chemistry, Clemson University, Clemson, SC 29634, TX, 78249, USA.
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Garcia
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil. wendell@ufg.br.|Instituto Nacional de Ciência e Tecnologia de Bioanalítica (INCTBio), Campinas, SP, Brazil. wendell@ufg.br.
authors.forename: Wendell K T
authors.initials: WK
authors.lastname: Coltro
mesh_terms: D001681:Biological Assay / Q000295:instrumentation / Q000379:methods; D015415:Biomarkers / Q000652:urine; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles; D010084:Oxidation-Reduction; D010209:Paper
chemical_list: D015415:Biomarkers
keywords: Clinical diagnostic; Colorimetric detection; Nanoparticle; Oxidation process; Urinalysis test