title: Salt stress affects mRNA editing in soybean chloroplasts.
abstract: Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA, NDHB, RPS14 and RPS16 genes presented differences in coverage and editing rates between control and salt-treated libraries. RT-qPCR assays demonstrated an increase in editing efficiency of selected genes. The salt stress enhanced the RNA editing process in transcripts, indicating responses to components of the electron transfer chain, photosystem and translation complexes. These increases can be a response to keep the homeostasis of chloroplast protein functions in response to salt stress.
authors.affiliation: Departamento de Genética, PPGBM, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Nureyev F
authors.initials: NF
authors.lastname: Rodrigues
authors.affiliation: Centro de Biotecnologia, PPGBCM, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Guilherme C da
authors.initials: GC
authors.lastname: Fonseca
authors.affiliation: Centro de Biotecnologia, PPGBCM, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Franceli R
authors.initials: FR
authors.lastname: Kulcheski
authors.affiliation: Departamento de Genética, PPGBM, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.|Centro de Biotecnologia, PPGBCM, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.|Departamento de Biofísica, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Margis