title: Diagnosis of Zika virus infection on a nanotechnology platform.
abstract: We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections.
authors.affiliation: Nirmidas Biotech, Palo Alto, California, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.|Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Pinsky
authors.affiliation: Nirmidas Biotech, Palo Alto, California, USA.
authors.forename: Jeyarama S
authors.initials: JS
authors.lastname: Ananta
authors.affiliation: Nirmidas Biotech, Palo Alto, California, USA.
authors.forename: Su
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Nirmidas Biotech, Palo Alto, California, USA.
authors.forename: Shylaja
authors.initials: S
authors.lastname: Arulkumar
authors.affiliation: Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wan
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Malaya K
authors.initials: MK
authors.lastname: Sahoo
authors.affiliation: Medical Research Institute, Colombo, Sri Lanka.
authors.forename: Janaki
authors.initials: J
authors.lastname: Abeynayake
authors.affiliation: Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Waggoner
authors.affiliation: Nirmidas Biotech, Palo Alto, California, USA.
authors.forename: Clay
authors.initials: C
authors.lastname: Hopes
authors.affiliation: Nirmidas Biotech, Palo Alto, California, USA.
authors.forename: Meijie
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California, USA.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Dai
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D003429:Cross Reactions; D003715:Dengue / Q000097:blood / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D036103:Nanotechnology; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D055815:Young Adult; D000071244:Zika Virus / Q000276:immunology; D000071243:Zika Virus Infection / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins