title: PowerPlex® Fusion 6C System: evaluation study for analysis of casework and database samples.
abstract: OBJECTIVE
  To report on the successful analysis of amplicons obtained with PowerPlex® Fusion 6C System, a highly robust 27-plex genotyping kit developed for human identification laboratories, on the Applied Biosystems® 3500 Genetic Analyzer.
  
  
  METHODS
  We performed characterization and evaluation studies following the Scientific Working Group on DNA Analysis Methods (SWGDAM) validation guidelines, examining several critical areas of kit performance. We report the results of sensitivity, robustness, heterozygous peak height ratio, precision, concordance, caseworks, and mixture interpretations. We tested sensitivity, using serial dilutions of control DNA.
  
  
  RESULTS
  The minimum amount of input DNA resulting in a full profile was 125 pg. Inhibition, inducted by urea, showsed a progressively fragmentation of DNA and a full profile was obtained until 1M of inhibitor factor. To test the profile quality, casework samples were extracted with different extraction methods: Chelex®100, QIAmp DNA Micro Kit and Phenol-Chloroform extraction. The results demonstrated that extraction chemistries do not have affect on amplification performance. Concordance check was performed by typing some casework samples and comparing the typing results with those obtained with other available kits. Thus, concordance was expected and supported by the data.
  
  
  CONCLUSIONS
  Reliable DNA typing results can be obtained using this new kit, demonstrating its effectiveness and utility in forensic analysis.
authors.forename: Selena
authors.initials: S
authors.lastname: Cisana
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Cerri
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bosetti
authors.affiliation: Andrea Verzeletti, Piazzale Spedali Civili 1, 25123 Brescia, Italy, andrea.verzeletti@unibs.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Verzeletti
authors.forename: Venusia
authors.initials: V
authors.lastname: Cortellini
mesh_terms: D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting / Q000379:methods* / Q000592:standards; D005838:Genotype; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D004247:DNA