title: Ki-67 antigen expression in the mammary epithelium of female rats in persistent estrus treated with anastrozole.
abstract: OBJECTIVE
  To evaluate Ki-67 antigen expression in mammary epithelium of female rats in persistent estrus treated with anastrozole.
  
  
  METHODS
  Twenty-eight Wistar-Hanover female rats in persistent estrus induced by subcutaneous injection of 1.25 mg of testosterone propionate in the second day of life were randomly divided into two groups, control and experimental, with 14 animals each. The animals of control group received only the vehicle (propyleneglycol) and the animals of group experimental received 0.125 mg daily of anastrozole by gavage during 28 days. After 28 days of treatment, all animals were sacrificed and the first pair of abdominal-inguinal mammary glands was removed and fixed in 10% buffered formalin to investigate Ki-67 antigen expression by immunohistochemistry.
  
  
  RESULTS
  The mean percentage of Ki-67-stained nuclei per 500 cells in the mammary epithelium was 76.97 ± 0.76 and 14.44 ± 2.02 [mean ± standard error of the mean (SEM)] in the control and experimental groups, respectively (p < 0.0001).
  
  
  CONCLUSIONS
  Anastrozole treatment significantly reduced Ki-67 expression in the mammary epithelium of rats in persistent estrus.
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.
authors.forename: Maria da Conceição
authors.initials: MDC
authors.lastname: Barros-Oliveira
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.
authors.forename: Danylo Rafhael
authors.initials: DR
authors.lastname: Costa-Silva
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.
authors.forename: Danielle Benigno de
authors.initials: DB
authors.lastname: Andrade
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.
authors.forename: Umbelina Soares
authors.initials: US
authors.lastname: Borges
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.
authors.forename: Vladimir Costa
authors.initials: VC
authors.lastname: Silva
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.
authors.forename: Rafael Soares
authors.initials: RS
authors.lastname: Borges
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.|b Mastology Unit of Getulio Vargas Hospital, Federal University of Piauí , Teresina , Brazil , and.
authors.forename: Pedro Vitor
authors.initials: PV
authors.lastname: Lopes-Costa
authors.affiliation: c Department of Statistics of Sao Paulo University , Sao Paulo , Brazil.
authors.forename: Airlane Pereira
authors.initials: AP
authors.lastname: Alencar
authors.affiliation: a Posgraduate Program of Sciences and Health, Federal University of Piauí , Teresina , Brazil.|b Mastology Unit of Getulio Vargas Hospital, Federal University of Piauí , Teresina , Brazil , and.
authors.forename: Benedito Borges
authors.initials: BB
authors.lastname: da Silva
mesh_terms: D000077384:Anastrozole; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D004971:Estrus / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D019394:Ki-67 Antigen / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D019394:Ki-67 Antigen; D009570:Nitriles; D014230:Triazoles; D000077384:Anastrozole; D013739:Testosterone
keywords: Anastrozole; Ki-67; aromatase inhibitor; persistent estrus; rat