title: Immunochemical Detection of α-Synuclein Autoantibodies in Parkinson's Disease: Correlation between Plasma and Cerebrospinal Fluid Levels.
abstract: Autoantibodies to Parkinson's disease (PD) amyloidogenic protein, α-synuclein, were recognized as a prospective biomarker for early disease diagnostics, yet there is inconsistency in previous reports, potentially related to PD status. Therefore, plasma and cerebrospinal fluid (CSF) of the cross-sectional cohort of 60 individuals, including recently diagnosed PD patients with mild and moderate PD and age-matched controls, were examined by enzyme-linked immunosorbent assay (ELISA). Nonparametric statistics was used for data analysis. We found significantly elevated levels of α-synuclein autoantibodies in both plasma and CSF in mild PD compared to controls, followed by some decrease in moderate PD. Receiver operating characteristic and effect size analyses confirmed the diagnostic power of α-synuclein antibodies in both plasma and CSF. For the first time, we showed the correlation between plasma and CSF α-synuclein antibody levels for mild, moderate, and combined PD groups. This indicates the potentiality of α-synuclein antibodies as PD biomarker and the increased diagnostic power of their simultaneous analysis in plasma and CSF.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University , Umeå 901 87, Sweden.
authors.forename: Istvan
authors.initials: I
authors.lastname: Horvath
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University , Umeå 901 87, Sweden.|Department of General Chemistry, Sumy State University , Sumy 40000, Ukraine.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Iashchishyn
authors.affiliation: Department of Pharmacology and Clinical Neuroscience, Umeå University , Umeå 901 87, Sweden.
authors.forename: Lars
authors.initials: L
authors.lastname: Forsgren
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University , Umeå 901 87, Sweden.
authors.forename: Ludmilla A
authors.identifier: 0000-0001-5886-2023
authors.initials: LA
authors.lastname: Morozova-Roche
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood* / Q000134:cerebrospinal fluid; D001324:Autoantigens / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000276:immunology; D051844:alpha-Synuclein / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D051844:alpha-Synuclein
keywords: Biomarkers; Parkinson’s disease; blood plasma; cerebrospinal fluid; enzyme-linked immunosorbent assay (ELISA); α-synuclein autoantibodies