title: Lipidomic characterization and localization of phospholipids in the human lung.
abstract: Lipids play a central role in lung physiology and pathology; however, a comprehensive lipidomic characterization of human pulmonary cells relevant to disease has not been performed. The cells involved in lung host defense, including alveolar macrophages (AMs), bronchial epithelial cells (BECs), and alveolar type II cells (ATIIs), were isolated from human subjects and lipidomic analysis by LC-MS and LC-MS/MS was performed. Additionally, pieces of lung tissue from the same donors were analyzed by MALDI imaging MS in order to determine lipid localization in the tissue. The unique distribution of phospholipids in ATIIs, BECs, and AMs from human subjects was accomplished by subjecting the large number of identified phospholipid molecular species to univariant statistical analysis. Specific MALDI images were generated based on the univariant statistical analysis data to reveal the location of specific cell types within the human lung slice. While the complex composition and function of the lipidome in various disease states is currently poorly understood, this method could be useful for the characterization of lipid alterations in pulmonary disease and may aid in a better understanding of disease pathogenesis.
authors.affiliation: Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045.
authors.forename: Karin A
authors.identifier: 0000-0002-7089-691X
authors.initials: KA
authors.lastname: Zemski Berry
authors.affiliation: Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045.
authors.forename: Robert C
authors.identifier: 0000-0002-9430-6515
authors.initials: RC
authors.lastname: Murphy
authors.affiliation: Department of Medicine, National Jewish Health, Denver, CO 80206.
authors.forename: Beata
authors.initials: B
authors.lastname: Kosmider
authors.affiliation: Department of Medicine, National Jewish Health, Denver, CO 80206 MasonB@NJHealth.org.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Mason
mesh_terms: D001692:Biological Transport; D002853:Chromatography, Liquid; D019295:Computational Biology; D006801:Humans; D008168:Lung / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010743:Phospholipids
keywords: alveolar macrophages; alveolar type II cells; bronchial epithelial cells