title: Cytochemical Localization of Polysaccharides inDendrobium officinaleand the Involvement ofDoCSLA6in the Synthesis of Mannan Polysaccharides.
abstract: Dendrobium officinaleis a precious traditional Chinese medicinal plant because of its abundant polysaccharides found in stems. We determined the composition of water-soluble polysaccharides and starch content inD.officinalestems. The extracted water-soluble polysaccharide content was as high as 35% (w/w). Analysis of the composition of monosaccharides showed that the water-soluble polysaccharides were dominated by mannose, to a lesser extent glucose, and a small amount of galactose, in a molar ratio of 223:48:1. Although starch was also found, its content was less than 10%. This result indicated that the major polysaccharides inD.officinalestems were non-starch polysaccharides, which might be mannan polysaccharides. The polysaccharides formed granules and were stored in plastids similar to starch grains, were localized inD.officinalestems by semi-thin and ultrathin sections.CELLULOSE SYNTHASE-LIKE A(CSLA) family members encode mannan synthases that catalyze the formation of mannan polysaccharides. To determine whether theCSLAgene fromD.officinalewas responsible for the synthesis of mannan polysaccharides,35S:DoCSLA6transgenic lines were generated and characterized. Our results suggest that theCSLAfamily genes fromD. officinaleplay an important role in the biosynthesis of mannan polysaccharides.
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: He
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Kunlin
authors.initials: K
authors.lastname: Wu
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Jianxia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Xuncheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Songjun
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Independent Researcher Miki-cho, Japan.
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Teixeira da Silva
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Rufang
authors.initials: R
authors.lastname: Deng
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Tan
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology Hefei, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis, Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Duan
keywords: CSLA; Dendrobium officinale; PAS; histology; polysaccharide granules