title: An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding.
abstract: Nucleic acid aptamers are a class of alternative ligands increasingly growing in importance in the face of contemporary detection challenges. Aptamers offer multiple advantages over traditional ligands like antibodies; however, their ability to specifically bind target molecules must first be confirmed after their generation. Use of a plate-based enzyme-linked aptamer sorbent assay (ELASA) is a generally rapid way to screen and characterize aptamer binding to protein targets. ELASA involves directly plating a protein target onto a nonspecific (polystyrene) surface and assessing binding of functionalized (biotinylated) aptamers to those plated proteins using an enzyme conjugate that recognizes the aptamers. Here, we describe an ELASA that was designed and used to evaluate and compare binding of ssDNA aptamers against the capsids of different strains of human norovirus.
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 315 Schaub Hall, 400 Dan Allen Drive, Raleigh, NC, 27695, USA. mdmoore5@ncsu.edu.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Moore
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 315 Schaub Hall, 400 Dan Allen Drive, Raleigh, NC, 27695, USA.
authors.forename: Blanca I
authors.initials: BI
authors.lastname: Escudero-Abarca
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 315 Schaub Hall, 400 Dan Allen Drive, Raleigh, NC, 27695, USA.
authors.forename: Lee-Ann
authors.initials: LA
authors.lastname: Jaykus
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001710:Biotin / Q000737:chemistry*; D036022:Capsid Proteins / Q000378:metabolism*; D004277:DNA, Single-Stranded; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D029322:Norovirus / Q000145:classification / Q000378:metabolism; D052156:SELEX Aptamer Technique / Q000295:instrumentation
chemical_list: D052157:Aptamers, Nucleotide; D036022:Capsid Proteins; D004277:DNA, Single-Stranded; D001710:Biotin
keywords: Aptamer; Aptamer binding; Binding evaluation; DNA-protein binding; Enzyme-linked aptamer sorbent assay; Plate assay