title: A comparative study of effect of autograft compared with allograft anterior cruciate ligament reconstruction on expressions ofLOXsandMMPs.
abstract: The present study aimed to compare the effect of autograft or allograft anterior cruciate ligament (ACL) reconstruction on the expressions of lipoxygenases (LOXs) and matrix metalloproteinases (MMPs) in a New Zealand white rabbit model. New Zealand white rabbits were divided randomly into control, sham, autograft and allograft groups. At the 4th and 8th week after operation, biomechanical testing was performed to measure the primary length, cross-sectional area, maximum tensile load and stiffness of ACL, and HE staining was used to observe cell morphology and fibre alignment of ACL. At the 2nd, 4th and 8th week after operation, quantitative real-time PCR (qRT-PCR), Western blotting and immunohistochemistry were applied to detectLOXsandMMPsexpressions, and expressions of adenomatous polyposis coli (APC)/Wnt signalling pathway-related proteins. At the 4th and 8th week after operation, the maximum tensile load and stiffness were higher in the autograft group than in the allograft group, and the values at the 8th week were higher than those at the 4th week after operation. The fibroblast proliferation in the allograft group was more significant than that in the autograft group. Compared with the control group,LOXsandMMPsexpressions and the positive expression rates ofLOXsandMMPsproteins were elevated, and the values in the allograft group were higher than those in the autograft group at all time points. At 8th week after operation, compared with the autograft group, Wnt expression was higher and APC expression was lower in the allograft group. Autograft and allograft ACL reconstruction can promoteLOXsandMMPsexpressions by activating the APC/Wnt signalling pathway.
authors.affiliation: Department of Orthopaedic Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, P.R. China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, P.R. China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ma
authors.affiliation: Department of Orthopaedic Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, P.R. China.
authors.forename: Shi-Bo
authors.initials: SB
authors.lastname: Huang
authors.affiliation: Department of Orthopaedic Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, P.R. China.
authors.forename: Liu-Bao
authors.initials: LB
authors.lastname: Ren
authors.affiliation: Department of Orthopaedic Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, P.R. China mrliuyupeng1988@163.com.
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Liu
mesh_terms: D064591:Allografts; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D059549:Anterior Cruciate Ligament Reconstruction / Q000379:methods*; D064592:Autografts; D001696:Biomechanical Phenomena; D005786:Gene Expression Regulation; D058945:Lipoxygenases / Q000032:analysis* / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011817:Rabbits; D014182:Transplantation, Autologous / Q000379:methods; D014184:Transplantation, Homologous / Q000379:methods; D060449:Wnt Signaling Pathway
chemical_list: D012333:RNA, Messenger; D058945:Lipoxygenases; D020782:Matrix Metalloproteinases
keywords: Allograft; Anterior cruciate ligament reconstruction; Autograft; LOXs; MMPs