title: Molecular Cloning and Characterization of Galactinol Synthases in Camellia sinensis with Different Responses to Biotic and Abiotic Stressors.
abstract: Galactinol synthase (GolS) is a key biocatalyst for the synthesis of raffinose family oligosaccharides (RFOs). RFOs accumulation plays a critical role in abiotic stress adaptation, but the relationship between expression of GolS genes and biotic stress adaptation remains unclear. In this study, two CsGolS genes were found to be highly up-regulated in a transcriptome library of Ectropic oblique-attacked Camellia sinensis. Three complete CsGolS genes were then cloned and characterized. Gene transcriptional analyses under biotic and abiotic stress conditions indicated that the CsGolS1 gene was sensitive to water deficit, low temperature, and abscisic acid, while CsGolS2 and CsGolS3 genes were sensitive to pest attack and phytohormones. The gene regulation and RFOs determination results indicated that CsGolS1 was primarily related to abiotic stress and CsGolS2 and CsGolS3 were related to biotic stress. GolS-mediated biotic stress adaptations have not been studied in depth, so further analysis of this new biological function is required.
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Rao
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Xungang
authors.initials: X
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Daxiang
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , 130 Changjiang Road West, Hefei, Anhui 230036, China.
authors.forename: Chaoling
authors.identifier: http://orcid.org/0000-0003-1300-2016
authors.initials: C
authors.lastname: Wei
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D028241:Camellia sinensis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D003080:Cold Temperature; D005700:Galactosyltransferases / Q000235:genetics* / Q000378:metabolism*; D007313:Insecta / Q000502:physiology; D010935:Plant Diseases / Q000469:parasitology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D005700:Galactosyltransferases; C096645:inositol 1-alpha-galactosyltransferase
keywords: Camellia sinensis; abiotic stress; biofunctional investigation; biotic stress; galactinol synthase; gene regulation