title: A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells.
abstract: There is a need for a general reporter system to study the interactions between a ligand and membrane protein in living cells. Here, we demonstrate a method that allows identification of undiscovered ligand partners of a membrane protein in its natural milieu. This assay will be of importance especially for multiple pass transmembrane proteins such as ion channels and GPCRs.
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, Mail Code: MB 10, 10550 N. Torrey Pines Rd, La Jolla, CA, 92037, USA.
authors.forename: Hongkai
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, Mail Code: MB 10, 10550 N. Torrey Pines Rd, La Jolla, CA, 92037, USA. rlerner@scripps.edu.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lerner
mesh_terms: D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D017479:Receptors, Platelet-Derived Growth Factor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D008024:Ligands; D011993:Recombinant Fusion Proteins; D017479:Receptors, Platelet-Derived Growth Factor; D010450:Endopeptidases; C099488:TEV protease
keywords: Interaction; Lentivirus; Ligand; Membrane protein; Proximity-based assay; Reporter cell