title: Immunohistochemical response in rats of beta-tricalcium phosphate (TCP) with or without BMP-2 in the production of collagen matrix critical defects.
abstract: This study aimed to assess the biological response of BMP-2 (bone morphogenetic protein-2) in supplementation with β-tricalcium phosphate (TCP) as a carrier in the bone healing of surgical defects in rats' calvaria. A critical-size defect (5mm in diameter) was filled with β-TCP alone or added with that plus 5mg of BMP-2 at 5, 15, and 30 postoperative days. Histomorphometric and immunohistochemical (osteocalcin, collagen type I, and metalloproteinase-9) analysis was performed to assess the features of bone healing. Histological behavior and collagen type I labeling showed increased formation of the collagen matrix, leading to a higher percentage of newly formed bone and biomaterial for tissue and more total mineralization of pure TCP when compared to the other groups. The supplementation with BMP-2 promoted faster TCP remodeling; however, there was no statistically significant difference for the bone formed in both groups (P>0.05). Collagen-matrix formation and new bone formation reached maximum levels when the defects were filled with pure TCP, even exceeding the levels from BMP-2 supplementation.
authors.affiliation: Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
authors.forename: Eloá Rodrigues
authors.initials: ER
authors.lastname: Luvizuto
authors.affiliation: Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
authors.forename: Júlio César Silva
authors.initials: JCS
authors.lastname: de Oliveira
authors.affiliation: Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil. Electronic address: pedroferreirabmf@gmail.com.
authors.forename: Pedro Henrique Silva
authors.initials: PHS
authors.lastname: Gomes-Ferreira
authors.affiliation: Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
authors.forename: Cassiano Costa Silva
authors.initials: CCS
authors.lastname: Pereira
authors.affiliation: Department of Surgery and Integrated Clinic, Sao Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
authors.forename: Leonardo Perez
authors.initials: LP
authors.lastname: Faverani
authors.affiliation: Department of Basic Sciences, Sao Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
authors.forename: Cristina
authors.initials: C
authors.lastname: Antoniali
authors.affiliation: Department of Basic Sciences, Sao Paulo State University (UNESP), School of Dentistry, Araçatuba, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects; D002130:Calcium Phosphates / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects / Q000293:injuries* / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D003094:Collagen
keywords: BMP; Collagen matrix; Histologic evaluation; Immunohistochemistry