title: Evaluation of antioxidant potential, enzyme inhibition activity and phenolic profile of Lathyrus cicera and Lathyrus digitatus: Potential sources of bioactive compounds for the food industry.
abstract: The genus Lathyrus has great importance in terms of food and agricultural areas. In this study, the in vitro antioxidant activity (phosphomolybdenum, DPPH, ABTS, FRAP, CUPRAC and metal chelating) and enzyme inhibitory activity evaluation (acetylcholinesterase, butyrylcholinesterase, α-amylase and α-glucosidase) of L. cicera and L. digitatus were investigated, as well as their phytochemical profiles. The screening of the main phytochemical compounds in aerial parts of L. cicera and L. digitatus was carried out by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn), observing that flavonoids represent the highest percentage of identified compounds, with abundance of tri- and tetra-glycosilated flavonoids, including acylated ones, especially in L. cicera. Generally, L. digitatus exhibited stronger antioxidant and enzyme inhibitory activities in correlation with its higher level of phenolics. The high number of phenolic compounds and the results of the antioxidant and enzyme assays suggest that these plants may be further used as sources of bioactive compounds, and for the preparation of new nutraceuticals.
authors.affiliation: Regional Institute for Applied Chemistry Research (IRICA), University of Castilla-La Mancha, Ciudad Real 13071, Spain.
authors.forename: E J
authors.initials: EJ
authors.lastname: Llorent-Martínez
authors.affiliation: Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, Jaén E-23071, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Ortega-Barrales
authors.affiliation: Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey. Electronic address: gokhanzengin@selcuk.edu.tr.
authors.forename: G
authors.initials: G
authors.lastname: Zengin
authors.affiliation: Department of Pharmaceutical Botany, "Iuliu Hatieganu" University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca, Romania; Institute for Life Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania. Electronic address: mocan.andrei@umfcluj.ro.
authors.forename: A
authors.initials: A
authors.lastname: Mocan
authors.affiliation: Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: M J
authors.initials: MJ
authors.lastname: Simirgiotis
authors.affiliation: Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Ceylan
authors.affiliation: Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Uysal
authors.affiliation: Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Aktumsek
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005503:Food Additives / Q000737:chemistry; D019649:Food Industry; D027807:Lathyrus / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D000516:alpha-Amylases / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D005503:Food Additives; D010636:Phenols; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases
keywords: Enzyme inhibitory; Lathyrus; Phenolics; Tandem mass spectrometry