title: Endothelial integrity may be regulated by a specific antigen via an IgE-mediated mechanism.
abstract: BACKGROUND
  Human vascular endothelial function and integrity may be regulated by many non-specific factors. However, the potential influence of specific antigens via an IgE-mediated mechanism remains unknown. The aim of the study was to determine the expression of the IgE receptors FcεRI and FcεRII in the human vascular endothelium and to assess their relevance in the IgE-mediated regulation of endothelial integrity.
  
  
  METHODS
  FcεRI and FcεRII expression in human umbilical vein endothelial cells (HUVEC) was genetically assessed by PCR with respective primers and sequencing. HUVEC were cultured with IL-4, and changes in FcεRI and FcεRII mRNA expression were analyzed by real-time PCR. Changes in the integrity of endothelium pre-treated with anti-BSA-DNP IgE following exposure to the specific BSA-DNP antigen was assessed using the Real-time Cell Electric Impedance Sensing system (RTCA-DP).
  
  
  RESULTS
  PCR and sequencing revealed the expression of FcεRI and FcεRII receptors in the human vascular endothelium. IL-4 caused respective 2- and 3-fold increases in FcεRI and FcεRII mRNA expression. Exposure of endothelium pre-treated with anti-BSA-DNP IgE to specific BSA-DNP antigen led to a 20% increase of endothelial integrity (p<0.05) after 24 hours, but only in cells pre-incubated with IL-4.
  
  
  CONCLUSIONS
  The presence of FcεRI and FcεRII may allow the human vascular endothelium to respond to a specific antigen by increasing its integrity via an IgE-mediated mechanism.
authors.affiliation: Department of Internal Diseases and Clinical Pharmacology, The Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Gorzelak-Pabis
authors.affiliation: Department of Internal Diseases and Clinical Pharmacology, The Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Luczak
authors.affiliation: Department of Internal Diseases and Clinical Pharmacology, The Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wojdan
authors.affiliation: Immunopathology and Genetics Laboratory, Department of Pediatrics, Oncology, Hematology and Diabetology, Medical University of Lodz, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Antosik
authors.affiliation: Immunopathology and Genetics Laboratory, Department of Pediatrics, Oncology, Hematology and Diabetology, Medical University of Lodz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Borowiec
authors.affiliation: Department of Internal Diseases and Clinical Pharmacology, The Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Poland.
authors.forename: Marlena
authors.initials: M
authors.lastname: Broncel
authors.affiliation: Department of Internal Diseases and Clinical Pharmacology, The Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Chalubinski
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology; D017455:Receptors, IgE / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D017455:Receptors, IgE; C026408:anti-IgE antibodies; D015847:Interleukin-4; D007073:Immunoglobulin E