title: 5-Hydroxymethylcytosine (5hmC) and Ten-eleven translocation 1-3 (TET1-3) proteins in the dorsal root ganglia of mouse: Expression and dynamic regulation in neuropathic pain.
abstract: Epigenetic mechanisms are increasingly implicated in chronic pain pathology. In this study, we demonstrate that the novel epigenetic mark 5-hydroxymethylcytosine (5hmC) is present in dorsal root ganglia (DRG) neurons and glia, and its levels increase following nerve injury. Furthermore, we show that the 5hmC-generating Ten-eleven translocation 1-3 (TET1-3) proteins are expressed in a cell-type specific manner in the DRG, with Tet3 displaying differential upregulation after injury, suggesting a potential role in neuropathic pain.
authors.affiliation: a Department of Anesthesiology , Duke University Medical Center , Durham , NC , USA.|b Department of Pharmacology and Cancer Biology , Duke University , Durham , NC , USA.|c Medical Scientist Training Program , Duke University School of Medicine , Durham , NC , USA.
authors.forename: Alexander G
authors.identifier: 0000-0002-7775-2062
authors.initials: AG
authors.lastname: Chamessian
authors.affiliation: a Department of Anesthesiology , Duke University Medical Center , Durham , NC , USA.
authors.forename: Yawar J
authors.initials: YJ
authors.lastname: Qadri
authors.affiliation: a Department of Anesthesiology , Duke University Medical Center , Durham , NC , USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Cummins
authors.affiliation: e Department of Anesthesiology , Cincinnati Children's Hospital , Cincinnati , OH , USA.
authors.forename: Michele
authors.initials: M
authors.lastname: Hendrickson
authors.affiliation: d Department of Anesthesiology , University of Cincinnati Medical Center , Cincinnati , OH , USA.
authors.forename: Temugin
authors.initials: T
authors.lastname: Berta
authors.affiliation: a Department of Anesthesiology , Duke University Medical Center , Durham , NC , USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Buchheit
authors.affiliation: a Department of Anesthesiology , Duke University Medical Center , Durham , NC , USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Van de Ven
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism*; D049308:Dioxygenases; D044127:Epigenesis, Genetic / Q000502:physiology*; D005727:Ganglia, Spinal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009437:Neuralgia / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; C540839:TET1 protein, mouse; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D049308:Dioxygenases; C551914:Tet2 protein, mouse; C551915:Tet3 protein, mouse
keywords: Chronic pain; epigenetics; neuropathic pain