title: Involvement of LuxS in Aeromonas salmonicida metabolism, virulence and infection in Atlantic salmon (Salmo salar L).
abstract: Quorum sensing is a bacterial density dependent communication system, which regarded to regulate co-operative behaviors of community and mediated by extracellular signal molecules named autoinducers (AI). Among various signals, autoinducer-2 (AI-2) is believed to be the messengers inter species and produced by LuxS. For Aeromonas salmonicida (A. salmonicida), an opportunistic pathogen to many cold-water teleost, little information has been known about the function of AI-2 and LuxS. Therefore, our aim was to preliminarily clarify the function of LuxS in A. salmonicida. The consequences demonstrated that wild type A. salmonicida exhibited AI-2 activity and luxS defective mutant strain fail to produce AI-2 signals. Furthermore, it was suggested that luxS deficiency could impact bacterial morphology, surface properties and virulence dramatically. Challenge experiment showed a tendency that immune factors expressed earlier when Atlantic salmon was infected with ΔluxS strain. Overall, we hypothesis that AI-2 quorum sensing could regulate the expression of A-layer protein coding gene vapA, and then influence bacterial survival ability when suffered from attack of the host immune system. Though additional studies are warranted, our study will supply a new thinking to control the damage caused by A. salmonicida.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China; University of Chinese Academy of Sciences, Beijing, 100039, PR China. Electronic address: qdmlj1219@outlook.com.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Meng
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China.
authors.forename: Yishuai
authors.initials: Y
authors.lastname: Du
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China; University of Chinese Academy of Sciences, Beijing, 100039, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: Dalian Ocean University, Dalian, 116023, PR China. Electronic address: yingliu@dlou.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
mesh_terms: D048409:Aeromonas salmonicida / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005667:Furunculosis / Q000276:immunology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D007783:Lactones / Q000378:metabolism*; D053038:Quorum Sensing; D020317:Salmo salar; D016415:Sequence Alignment / Q000662:veterinary; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria
keywords: A-layer; AI-2; Aeromonas salmonicida; Innate immnuity; LuxS; Quorum sensing; Salmo salar L; Surface property; Virulence