title: Imaging the Effector CD8 Synapse.
abstract: Here, we describe 4D imaging of effector CD8+ T cells as they conjugate and kill live targets in vitro and analyze the polarization dynamics of intracellular compartments to this cell-cell interface.
authors.affiliation: Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK.
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Frazer
authors.affiliation: Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Asano
authors.affiliation: Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK. gg305@cam.ac.uk.
authors.forename: Gillian M
authors.initials: GM
authors.lastname: Griffiths
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D054992:Immunological Synapses / Q000276:immunology*; D018613:Microscopy, Confocal / Q000379:methods
keywords: 4D object-based image analysis; Imaris; Nucleofection; Time-lapse confocal imaging