title: Evaluation of a novel fluorescent nanobeacon for targeted imaging of Thomsen-Friedenreich associated colorectal cancer.
abstract: The Thomsen-Friedenreich (TF) antigen represents a prognostic biomarker of colorectal carcinoma. Here, using a nanobeacon, the surface of which was fabricated with peanut agglutinin as TF-binding molecules, we demonstrate that the nanobeacon is able to detect TF antigen in frozen and freshly biopsied polyps using fluorescence microscopy. Our results provide important clues about how to detect aberrant colonic tissues in the most timely fashion. Given the versatile application method for this topical nanobeacon, the protocol used in this work is amenable to clinical colonoscopy. Moreover, the prospects of clinical translation of this technology are evident.
authors.affiliation: Division of Endoscopy, Kyoto University Hospital, Kyoto, Japan; Department of Gastroenterology and Hepatology, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakase
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakuma
authors.affiliation: Gastroenterology and Hepatology, Osakafu Saiseikai Nakatsu Hospital, Osaka, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Fukuchi
authors.affiliation: Division of Endoscopy, Kyoto University Hospital, Kyoto, Japan; Division of Inflammatory Bowel Disease, Digestive Disease Center, Kitano Hospital, Osaka, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoshino
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan.
authors.forename: Kohta
authors.initials: K
authors.lastname: Mohri
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan; Advanced Materials R&D Laboratory, ADEKA Corp., Tokyo, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Miyata
authors.affiliation: Advanced Materials R&D Laboratory, ADEKA Corp., Tokyo, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Kumagai
authors.affiliation: Advanced Materials R&D Laboratory, ADEKA Corp., Tokyo, Japan.
authors.forename: Ken-Ichiro
authors.initials: KI
authors.lastname: Hiwatari
authors.affiliation: Advanced Materials R&D Laboratory, ADEKA Corp., Tokyo, Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Tsubaki
authors.affiliation: Advanced Materials R&D Laboratory, ADEKA Corp., Tokyo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ikejima
authors.affiliation: Advanced Materials R&D Laboratory, ADEKA Corp., Tokyo, Japan.
authors.forename: Etsuo
authors.initials: E
authors.lastname: Tobita
authors.affiliation: Department of Radiology, Vanderbilt University Institute of Imaging Science.
authors.forename: Meiying
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Radiology, Vanderbilt University Institute of Imaging Science.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Wilson
authors.affiliation: Division of Gastrointestinal and Hepatic Pathology, Vanderbilt School of Medicine.
authors.forename: Kay
authors.initials: K
authors.lastname: Washington
authors.affiliation: Department of Radiology, Vanderbilt University Institute of Imaging Science; Department of Radiology and Radiological Sciences; Department of Biomedical Engineering; Vanderbilt Ingram Cancer Center, Vanderbilt School of Medicine; Vanderbilt Brain Institute; Molecular Physiology and Biophysics.
authors.forename: John C
authors.initials: JC
authors.lastname: Gore
authors.affiliation: Department of Radiology, Vanderbilt University Institute of Imaging Science; Department of Radiology and Radiological Sciences; Department of Biomedical Engineering; Vanderbilt Ingram Cancer Center, Vanderbilt School of Medicine; Vanderbilt Brain Institute; Molecular Physiology and Biophysics; Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN, USA.
authors.forename: Wellington
authors.initials: W
authors.lastname: Pham
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000473:pathology; D000236:Adenoma / Q000175:diagnosis / Q000473:pathology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging; D019887:Peanut Agglutinin / Q000737:chemistry
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D005456:Fluorescent Dyes; D015335:Molecular Probes; D019887:Peanut Agglutinin; C016467:Thomsen-Friedenreich antigen
keywords: TF antigen; colorectal cancer; fluorescence colonoscopy; nanobeacon