title: Identification of the cysteine-rich 61 (CYR61) gene variations in osteosarcoma patients.
abstract: Osteosarcoma requires an angiogenesis process. CYR61 is one of the extracellular signaling molecules that promote angiogenesis, tumor growth, and the malignancy of osteosarcoma. In the present study, we investigate the CYR61 gene variations in osteosarcoma and their correlations with clinicopathological findings.
  
  
  
  We performed variation analysis of the CYR61 gene in 58 patients with osteosarcoma. With an aim to ascertain the variety of variations in exons 2, 3, 4, and 5 of the CYR61 gene in osteosarcoma, we did a PCR-SSCP followed by DNA sequencing.
  
  
  
  In osteosarcoma the CYR61 gene variations found were 18.96% (11/58) in exon 2, 3.44% (2/58) in exon 3, 8.62% (5/58) in exon 4, and 15.51% (9/58) in exon 5. In our variation analysis, we detected one missense variation in exon 2 (Arg47Trp), one silent variation in exon 3 (Lys152Lys), one missense variation in exon 4 (Phe213Leu), and two missense variations in exon 5 (Gly315Arg and Asp339Asn). The overall CYR61 variation frequency in exons 2, 3, 4, and 5 was determined to be 46.55% (27/58).
  
  
  
  Our study specifies the role of CYR61 gene variation in osteosarcoma. The result signifies that CYR61 might be used as a prognostic/diagnostic marker in osteosarcoma patients.
authors.affiliation: Department of Orthopedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Syed Rizwan
authors.initials: SR
authors.lastname: Hussain
authors.affiliation: Department of Orthopedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Sabir
authors.initials: S
authors.lastname: Ali
authors.affiliation: Department of Orthopedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Ajai
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Orthopedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Vineet
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Orthopedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Nazia
authors.initials: N
authors.lastname: Rizivi
authors.affiliation: Department of Orthopedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Yadav
authors.affiliation: Department of Molecular Cell Biology Lab, Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Mohammad Kaleem
authors.initials: MK
authors.lastname: Ahmad
authors.affiliation: Department of Oncology, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Molecular Cell Biology Lab, Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Mahdi
mesh_terms: D016022:Case-Control Studies; D055515:Cysteine-Rich Protein 61 / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D006801:Humans; D020125:Mutation, Missense / Q000235:genetics; D012516:Osteosarcoma / Q000453:epidemiology* / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics
chemical_list: C501848:CCN1 protein, human; D055515:Cysteine-Rich Protein 61; D004247:DNA