title: [Morphofunctional changes of dendritic cells induced by sulfated polysaccharides of brown algae].
abstract: The effects of various sulfated polysaccharides of brown algae Fucus evanescens, Saccharina cichorioides and Saccharina japonica on the morphofunctional changes of dendritic cells have been investigated using flow cytometry and phase-contrast microscopy. The dendritic cells are characterized by larger sizes, vacuolated cytoplasm, eccentrically located nucleus, and also by the presence of numerous cytoplasmic pseudopodia of various shapes. They express surface markers, indicating their maturation (CD83, CD11c, HLA-DR, CD86). Increased production of immunoregulatory (IL-12) and proinflammatory TNF-a, IL-6) cytokines (by dendritic cells polarizes the development of the Th-1 type immune response.
authors.affiliation: Somov Scientific Research Institute of Epidemiology and Microbiology, Vladivostok, Russia.
authors.forename: I D
authors.initials: ID
authors.lastname: Makarenkova
authors.affiliation: Mechnikov Scientific Research Institute of Vaccines and Sera, Moscow, Russia.
authors.forename: N K
authors.initials: NK
authors.lastname: Akhmatova
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, FEB RUS Vladivostok, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Ermakova
authors.affiliation: Somov Scientific Research Institute of Epidemiology and Microbiology, Vladivostok, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Besednova
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D051940:B7-2 Antigen / Q000235:genetics / Q000276:immunology; D039521:CD11c Antigen / Q000235:genetics / Q000276:immunology; D002240:Carbohydrate Sequence; D002454:Cell Differentiation; D048429:Cell Size; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D061251:Primary Cell Culture; D011554:Pseudopodia / Q000187:drug effects* / Q000648:ultrastructure; D013463:Sulfuric Acid Esters / Q000737:chemistry; D058408:Th1-Th2 Balance / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; D039521:CD11c Antigen; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D018664:Interleukin-12; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D011134:Polysaccharides; D013463:Sulfuric Acid Esters; D014409:Tumor Necrosis Factor-alpha; D000097802:CD83 Antigen
keywords: cytokines; dendritic cells; fucoidan; morphology; sulfated polysaccharides