title: A new eremophilane glycoside from the fruits of Physalis pubescens and its cytotoxic activity.
abstract: Physalis pubescens L. has been used as a traditional folk medicine in China. Comprehensive studies aimed at identifying the bioactive constituents are still lacking. In the course of searching bioactive secondary metabolites from P. pubescens L., phytochemical investigation of the fruits of P. pubescens L. led to the isolation of one new eremophilane glycoside, 1β,3α-dihydroxy-7α-eremophila-911-dien-3-O-[α-L-arabinopyranosyl-(l→6)]-β-D-glucopyranoside (1), and six known glycosides (2-7). Their structures were established via extensive spectroscopic data including 1D and 2D NMR and HRESIMS. Cytotoxic effects against six human cancer cell lines (C4-2B, A375, A375-S2, 786-O, Caki-2, and ACHN) and one human normal hepatic cell line (L02) were evaluated and compounds 1 and 2 exhibited moderate cytotoxicity against C4-2B and A375 cell lines with IC50values in the range of 6.78-28.3 μM. The study indicates that the fruits of P. pubescens L. have the potential to be used for drug discovery.
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.|b Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica , Tianjin University of Traditional Chinese Medicine , Tianjin , People's Republic of China.
authors.forename: Guiyang
authors.initials: G
authors.lastname: Xia
authors.affiliation: b Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica , Tianjin University of Traditional Chinese Medicine , Tianjin , People's Republic of China.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Xia
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Wang
authors.affiliation: b Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica , Tianjin University of Traditional Chinese Medicine , Tianjin , People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.affiliation: b Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica , Tianjin University of Traditional Chinese Medicine , Tianjin , People's Republic of China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Ding
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Chen
authors.affiliation: a Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Department of Natural Products Chemistry , School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang , People's Republic of China.|b Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica , Tianjin University of Traditional Chinese Medicine , Tianjin , People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D032321:Physalis / Q000737:chemistry* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry / Q000378:metabolism; D064210:Secondary Metabolism; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D012717:Sesquiterpenes
keywords: Eremophilane glucoside; Physalis pubescens; cytotoxic activity