title: [Preliminary study on the role of voltage-gated sodium channel subtype Nav1.5 in lymph node metastasis of oral squamous cell carcinoma].
abstract: Objective:To investigate the relationship between the expression of voltage-gated sodium channel subtype Nav1.5 in oral squamous cell carcinoma (OSCC) and the occurrence and lymph node metastasis of OSCC.Methods:Totally 10 samples of normal oral mucosa tissue as control group, 26 samples of OSCC as the experimental group was divided into non-metastatic group (n=16) and metastatic group (n=10) according to the presence or absence of lymph node metastasis. Quantitative real-time PCR (qPCR), Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of Nav1.5 in control group and experimental groups at mRNA and protein levels. The data were analyzed by one-way analysis.Results:The expression of Nav1.5 mRNA in the experimental group (non-metastatic group: 2.311±0.134, metastatic group: 4.462±0.362) was higher than those in the control group (1.054±0.162) (P=0.037;P=0.029), and the metastasis group was significantly higher than the non-metastasis group (P=0.031). Western blotting showed the expression of Nav1.5 in experimental groups (non-metastatic: 0.143±0.005, metastatic: 0.253±0.015) was up-regulated significantly compared with control group (0.080±0.010) (P=0.034,P=0.026), and the metastasis group was significantly higher than the non-metastasis group (P=0.033). The immunohistochemistry show the positive expression rates of Nav1.5 in normal and OSCC tissues were 1/10 and 92% (24/26). The differences were statistically significant (P=0.016), and the metastasis group was significantly higher than the non-metastasis group (P=0.028). The ELISA results revealed that the level of Nav1.5 in control was control group (0.834±0.103) μg/L, in non-metastasis group was (1.578±0.167) μg/L, in metastasis group was (3.882±0.422) μg/L (P=0.041;P=0.032), and the metastasis group was significantly higher than the non-metastasis group (P=0.030).Conclusions:Nav1.5 was highly expressed in poorly differentiated OSCC and the expression was significantly different with or without lymph node metastasis. Nav1.5 may be involved in the occurrence and metastasis of OSCC.
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Zhu
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000556:secondary*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes; D008207:Lymphatic Metastasis; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology*; D062554:NAV1.5 Voltage-Gated Sodium Channel / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D062554:NAV1.5 Voltage-Gated Sodium Channel; D012333:RNA, Messenger
keywords: Carcinoma, squamous cell; Lymphatic metastasis; Sodium channels, voltage-gated