title: Parental High-Fat Diet Promotes Inflammatory and Senescence-Related Changes in Prostate.
abstract: Background. Obesity and dietary habits are associated with increased incidences of aging-related prostatic diseases. The present study was aimed to investigate transgenerational effects of chronic high-fat diet (HFD) feeding on inflammation and senescence-related changes in prostate.Methods. Sprague-Dawley rats were kept on either normal or HFD one. Senescence-associatedβ-galactosidase (SAβ-gal) activity, inflammation, and cellular proliferation were determined in the prostate.Results. Increased SAβ-gal activity, expression of p53, and cell proliferation marker PCNA were observed in ventral prostate of HFD-fed rats. Immunostaining for p53 and PCNA revealed that the p53 immunopositive cells were primarily in stroma while PCNA immunopositive cells were epithelial cells. An increase in expression of cycloxygenase-2 (COX-2) and phosphorylation of nuclear factor-kappa B (NF-kB) was observed in prostate of weaning pups HFD-fed parents. However, in adult pups, irrespective of dietary habit, a significant increase in the expression of COX-2, PCNA, phosphorylation of NF-kB, infiltration of inflammatory cells, and SAβ-gal activity was observed.Conclusions.Present investigation reports that HFD feeding promotes accumulation of p53 expressing cells, proliferation of epithelial cells, and senescence-related changes in prostate. Further, parental HFD-feeding upholds inflammatory, proliferative, and senescence-related changes in prostate of pups.
authors.affiliation: Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India.
authors.forename: Kulbhushan
authors.identifier: 0000-0003-3061-9739
authors.initials: K
authors.lastname: Tikoo
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India.
authors.forename: Ajit
authors.identifier: 0000-0003-3724-3842
authors.initials: A
authors.lastname: Vikram
authors.affiliation: Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Shrivastava
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India.
authors.forename: Gopabandhu
authors.initials: G
authors.lastname: Jena
authors.affiliation: Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India.
authors.forename: Heta
authors.initials: H
authors.lastname: Shah
authors.affiliation: Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Chhabra
mesh_terms: D000818:Animals; D016922:Cellular Senescence; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D007249:Inflammation / Q000209:etiology* / Q000473:pathology; D007333:Insulin Resistance; D008297:Male; D009765:Obesity / Q000209:etiology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology*; D011467:Prostate / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley