title: Designer bFGF-incorporated d-form self-assembly peptide nanofiber scaffolds to promote bone repair.
abstract: d-Form and l-form peptide nanofiber scaffolds can spontaneously form stable β-sheet secondary structures and nanofiber hydrogel scaffolds, and hold some promise in hemostasis and wound healing. We report here on the synthetic self-assembling peptide d-RADA16 and l-RADA16 are both found to produce stable β-sheet secondary structure and nanofiber hydrogel scaffolds based on circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM) and rheology analysis etc. d-RADA16 hydrogel and l-RADA16 hydrogel can enhance obvious bone repair in femoral condyle defects of the Sprague-Dawley (SD) rat model compared to PBS treatment. Based on micro-computed tomography (CT), it was revealed that d-RADA16 hydrogel and l-RADA16 hydrogel were capable to obtain the extensive bone healing. Histological evaluation also found that these two hydrogels facilitate the presence of more mature bone tissue within the femoral condyle defects. Additionally, d-RADA16 hydrogel showed some potential in storing and releasing basic-fibroblast growth factor (bFGF) which was able to further promote bone regeneration based on micro-CT analysis. These results indicate that d-form peptide nanofiber hydrogel have some special capacity for bone repair.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address: binheing@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Ou
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Weikang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ao
authors.initials: A
authors.lastname: Zhou
authors.affiliation: Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jinqiu
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Physical Science and Technology, Sichuan University, Chengdu 610000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Dianming
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address: 568731668@qq.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology; D002942:Circular Dichroism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides; D016222:Fibroblast Growth Factor 2
keywords: Bone repair; Molecular self-assembly; Nanofiber hydrogel scaffold; d-Form peptide; l-Form peptide