title: Analyzing the Dynamics of Signaling Microclusters.
abstract: T cell antigen receptor (TCR) stimulation induces recruitment and accumulation of various types of signaling molecules and forms signaling microclusters. The dynamics of the microclusters are important for regulating the quality and quantity of T cell activation. We describe here our protocols for analysis of signaling microclusters by using supported planar bilayers.
authors.affiliation: Laboratory for Cell Signaling, RIKEN Center for Integrative Medical Sciences., 1 7 22 Suehiro-cho, Yokohama, 230 0045, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Hashimoto-Tane
authors.affiliation: Laboratory for Cell Signaling, RIKEN Center for Integrative Medical Sciences., 1 7 22 Suehiro-cho, Yokohama, 230 0045, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yokosuka
authors.affiliation: Laboratory for Cell Signaling, RIKEN Center for Integrative Medical Sciences., 1 7 22 Suehiro-cho, Yokohama, 230 0045, Japan. takashi.saito@riken.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Saito
mesh_terms: D000818:Animals; D021961:Cell Membrane Structures / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: Confocal microscopy; Phosphorylation; Retroviral expression system; Signal; Supported planar bilayers; TCR; TIRF microscopy; Time-lapse imaging