title: n-Butanol production from sucrose and sugarcane juice by engineered Clostridium tyrobutyricum overexpressing sucrose catabolism genes and adhE2.
abstract: The production of n-butanol from sugarcane juice by metabolically engineered Clostridium tyrobutyricum Ct(Δack)-pscrBAK overexpressing scr operon genes (scrB, scrA, and scrK) for sucrose catabolism and an aldehyde/alcohol dehydrogenase gene (adhE2) for butanol biosynthesis was studied with corn steep liquor (CSL) as a low-cost nitrogen source. In free cell fermentation, butanol production of ∼16g/L at a yield of 0.31±0.02g/g and productivity of 0.33±0.02g/L·h was obtained from sucrose and yield of 0.24±0.02g/g and productivity of 0.30±0.01g/L·h from sugarcane juice containing sucrose, glucose and fructose. The fermentation was also studied in a fibrous bed bioreactor (FBB) operated in a repeated batch mode for 10 consecutive cycles in 10days, achieving an average butanol yield of 0.21±0.02g/g and productivity of 0.53±0.05g/L·h from sugarcane juice, demonstrating its long-term stability without applying the antibiotic selection pressure.
authors.affiliation: Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing 100083, PR China; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
authors.forename: Jianzhi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
authors.forename: Le
authors.initials: L
authors.lastname: Yu
authors.affiliation: Bioprocessing Innovative Company, 4734 Bridle Path Ct., Dublin, OH 43017, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Lin
authors.affiliation: Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Qiaojuan
authors.initials: Q
authors.lastname: Yan
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA. Electronic address: yang.15@osu.edu.
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D048014:Clostridium tyrobutyricum / Q000378:metabolism*; D005285:Fermentation; D031786:Saccharum / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D013395:Sucrose; D020001:1-Butanol
keywords: Butanol; Clostridium tyrobutyricum; Fibrous bed bioreactor; Metabolic engineering; Sucrose catabolism; Sugarcane juice