title: The potential impact of recent insights into proteomic changes associated with glaucoma.
abstract: Glaucoma, a major ocular neuropathy, is still far from being understood on a molecular scale. Proteomic workflows revealed glaucoma associated alterations in different eye components. By using state-of-the-art mass spectrometric (MS) based discovery approaches large proteome datasets providing important information about glaucoma related proteins and pathways could be generated. Corresponding proteomic information could be retrieved from various ocular sample species derived from glaucoma experimental models or from original human material (e.g. optic nerve head or aqueous humor). However, particular eye tissues with the potential for understanding the disease's molecular pathomechanism remains underrepresented. Areas covered: The present review provides an overview of the analysis depth achieved for the glaucomatous eye proteome. With respect to different eye regions and biofluids, proteomics related literature was found using PubMed, Scholar and UniProtKB. Thereby, the review explores the potential of clinical proteomics for glaucoma research. Expert commentary: Proteomics will provide important contributions to understanding the molecular processes associated with glaucoma. Sensitive discovery and targeted MS approaches will assist understanding of the molecular interplay of different eye components and biofluids in glaucoma. Proteomic results will drive the comprehension of glaucoma, allowing a more stringent disease hypothesis within the coming years.
authors.affiliation: a Experimental Ophthalmology , University Medical Center , Mainz , Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Funke
authors.affiliation: a Experimental Ophthalmology , University Medical Center , Mainz , Germany.
authors.forename: Natarajan
authors.initials: N
authors.lastname: Perumal
authors.affiliation: a Experimental Ophthalmology , University Medical Center , Mainz , Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Bell
authors.affiliation: a Experimental Ophthalmology , University Medical Center , Mainz , Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Pfeiffer
authors.affiliation: a Experimental Ophthalmology , University Medical Center , Mainz , Germany.
authors.forename: Franz H
authors.initials: FH
authors.lastname: Grus
mesh_terms: D001082:Aqueous Humor / Q000378:metabolism; D005901:Glaucoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D013058:Mass Spectrometry; D009898:Optic Disk / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000235:genetics*; D040901:Proteomics
chemical_list: D020543:Proteome
keywords: Glaucoma; discovery; eye proteome; mass spectrometry; ocular tissues; protein biomarkers; proteome alteration; proteomics