title: The efficacy of WGA modified daunorubicin anti-resistant liposomes in treatment of drug-resistant MCF-7 breast cancer.
abstract: BACKGROUND
  Breast cancer is the most common malignancy and remains a leading cause of cancer-related deaths in female. Chemotherapy failure of breast cancer is mainly associated with multidrug resistance of cancer cells.
  
  
  OBJECTIVE
  The WGA modified daunorubicin anti-resistant liposomes were developed for circumventing the multidrug resistance and eliminating cancer cells.
  
  
  METHODS
  WGA was modified on liposomal surface for increasing the intracellular uptake. Tetrandrine was inserted into the phospholipid bilayer for reversing cancer drug-resistance, and daunorubicin was encapsulated in liposomal aqueous core as an anticancer agent. Evaluations were performed on MCF-7 cells, MCF-7/ADR cells and xenografts of MCF-7/ADR cells.
  
  
  RESULTS
  In vitro results showed that WGA modified daunorubicin anti-resistant liposomes exhibited suitable physicochemical properties, significantly increased intracellular uptake in both MCF-7 cells and MCF-7/ADR cells, and circumvented the multidrug resistance via inhibiting P-gp. In vivo results demonstrated that the targeting liposomes showed a long-circulatory effect in blood system, and could remarkably accumulate at the tumor location. The involved action mechanisms for the enhanced anticancer efficacy were activation of pro-apoptotic proteins (Bax and Bok), apoptotic enzymes (caspase 8, caspase 9 and caspase 3).
  
  
  CONCLUSIONS
  The established WGA modified daunorubicin anti-resistant liposomes could provide a potential strategy for treating resistant MCF-7 breast cancer.
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Song
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.affiliation: a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
authors.forename: Xue-Tao
authors.initials: XT
authors.lastname: Li
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003630:Daunorubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D008081:Liposomes; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014909:Wheat Germ Agglutinins / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000903:Antibiotics, Antineoplastic; D008081:Liposomes; D014909:Wheat Germ Agglutinins; D003630:Daunorubicin
keywords: WGA; breast cancer; daunorubicin; multidrug resistance; tetrandrine