title: The molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L.
abstract: Myosin Va (MyoVa) is an actin-based molecular motor abundantly found at the centrosome. However, the role of MyoVa at this organelle has been elusive due to the lack of evidence on interacting partners or functional data. Herein, we combined yeast two-hybrid screen, biochemical studies and cellular assays to demonstrate that MyoVa interacts with RPGRIP1L, a cilia-centrosomal protein that controls ciliary signaling and positioning. MyoVa binds to the C2 domains of RPGRIP1L via residues located near or in the Rab11a-binding site, a conserved site in the globular tail domain (GTD) from class V myosins. According to proximity ligation assays, MyoVa and RPGRIP1L can interact near the cilium base in ciliated RPE cells. Furthermore, we showed that RPE cells expressing dominant-negative constructs of MyoVa are mostly unciliated, providing the first experimental evidence about a possible link between this molecular motor and cilia-related processes.
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.|Graduate Program in Functional and Molecular Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
authors.forename: L H P
authors.initials: LH
authors.lastname: Assis
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: R M P
authors.initials: RM
authors.lastname: Silva-Junior
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.|Graduate Program in Functional and Molecular Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
authors.forename: L G
authors.initials: LG
authors.lastname: Dolce
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.
authors.forename: M R
authors.initials: MR
authors.lastname: Alborghetti
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.
authors.forename: R V
authors.initials: RV
authors.lastname: Honorato
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.|Graduate Program in Functional and Molecular Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
authors.forename: A F Z
authors.initials: AF
authors.lastname: Nascimento
authors.affiliation: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
authors.forename: T D
authors.initials: TD
authors.lastname: Melo-Hanchuk
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.
authors.forename: D M
authors.initials: DM
authors.lastname: Trindade
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.
authors.forename: C C C
authors.initials: CC
authors.lastname: Tonoli
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: C T
authors.initials: CT
authors.lastname: Santos
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.
authors.forename: P S L
authors.initials: PS
authors.lastname: Oliveira
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: R E
authors.initials: RE
authors.lastname: Larson
authors.affiliation: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Kobarg
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Espreafico
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.
authors.forename: P O
authors.initials: PO
authors.lastname: Giuseppe
authors.affiliation: Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials, Campinas, SP, Brazil.
authors.forename: M T
authors.initials: MT
authors.lastname: Murakami
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001665:Binding Sites; D018385:Centrosome / Q000378:metabolism; D002923:Cilia / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D006801:Humans; D008958:Models, Molecular; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D024701:Myosin Type V / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C532826:RPGRIP1L protein, human; D011994:Recombinant Proteins; C077511:MYO5A protein, human; D024701:Myosin Type V; D018995:Myosin Heavy Chains