title: Backbone and side-chain resonance assignments for the tmRNA-binding protein, SmpB, from Mycobacterium tuberculosis.
abstract: Small protein B (SmpB) is an essential molecule in trans-translation which is a universal biological pathway for protein synthesis in bacteria. Trans-translation can release stalled ribosomes from defective mRNAs and target tag-protein fragments for degradation, and then restart protein synthesis. The SmpB-tmRNA complex coordinating with other components of the trans-translation system, plays vital roles in Mycobacterium tuberculosis under both stress conditions and non-replicating conditions. Thus, elucidation of molecular details and dynamic properties of the SmpB-tmRNA interaction is a crucial step towards effectively blocking trans-translation process to shorten the duration of tuberculosis treatment. Here, we report resonance assignments for1H,13C and15N of M. tuberculosis SmpB (MtSmpB, spanning residues 4-133) protein determined by a suite of 2D/3D heteronuclear NMR experiments along with predicted the secondary structure.
authors.affiliation: Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian, 350108, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Yindi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian, 350108, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian, 350108, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Meng
authors.affiliation: MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. dhlin@xmu.edu.cn.
authors.forename: Donghai
authors.initials: D
authors.lastname: Lin
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D009169:Mycobacterium tuberculosis; D019906:Nuclear Magnetic Resonance, Biomolecular; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D016601:RNA-Binding Proteins; C430328:small protein B
keywords: MtSmpB; NMR resonance assignment; Secondary structure prediction; Trans-translation; Tuberculosis