title: Effects of cultured Cordyceps mycelia polysaccharide A on tumor neurosis factor-α induced hepatocyte injury with mitochondrial abnormality.
abstract: Cordyceps sinensis mycelia polysaccharide A (CPS-A), was isolated from cultured Cordyceps mycelia by 65% alcohol extraction and ion-exchange column chromatography. The molecular weight of CPS-A was 1.2×104Da and the backbone was mainly composed of (1→2)-linked β-d-mannopyranose, (1→2,4)-linked β-d-mannopyranose and (1→4)-linked α-d-glucopyranose with terminal β-d-mannopyranose and α-d-glucopyranose residues. CPS-A played a protective role against TNF-α induced mitochondria injury in L02 cells via up-regulation of mitofusin 2, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), and membrane potential. CPS-A also played a protective role against TNF-α induced L02 cells apoptosis via up-regulation of Bcl-2 and down-regulation of Bid, Bax, cleaved caspase-3, cleaved caspase-9 and ROS production. Moreover, CPS-A attenuated both the normal expression and overexpression of TNF-α receptor 1 (TNFR1) induced by TNF-α administration. In conclusion, CPS-A was involved in TNF-α induced mitochondria abnormality via TNFR1/ROS/Mfn2 pathway.
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China; Department of Pharmacy, Jiangsu Food & Pharmaceutical Science College, Huaian 223003, People's Republic of China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Tang
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Weikun
authors.initials: W
authors.lastname: Wei
authors.affiliation: Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210046, People's Republic of China.
authors.forename: Wang
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Zhengqi
authors.initials: Z
authors.lastname: Zha
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Luo
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Yin
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China. Electronic address: hfj@cpu.edu.cn.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Huang
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China. Electronic address: ywang@cpu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D032661:Cordyceps / Q000737:chemistry*; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000473:pathology*; D011134:Polysaccharides / Q000494:pharmacology*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000009:adverse effects
chemical_list: D011134:Polysaccharides; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha
keywords: Apoptosis; Cordyceps sinensis mycelia polysaccharide A; Mitochondria abnormality; Mitofusin 2; TNF-α receptor 1