title: miR-422a Inhibits Glioma Proliferation and Invasion by Targeting IGF1 and IGF1R.
abstract: Glioma is a common type of malignant brain tumor characterized by aggressive metastasis capability. Recent evidence has suggested that noncoding RNAs, including microRNAs, have important functions in the pathophysiology of glioma development. In this study, we investigated the biological function of miR-422a in human glioma. We found that miR-422a was downregulated in glioma tissues. We also demonstrated that expression of miR-422a in glioma cells markedly suppressed cell proliferation, migration, and invasion. In addition, we identified insulin-like growth factor 1 (IGF1) and IGF1 receptor (IGF1R) as inhibitory targets of miR-422a in glioma cells. We established that the expression levels of miR-422a were negatively correlated with the expression levels of IGF1/IGF1R and the clinical parameters in glioma patients. An IGFR inhibitor, AG1024, completely blocked the activity of miR-442a on glioma cell proliferation and invasion, which further confirmed that miR-422a functions through IGF1 and IGF1R.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Wang
authors.forename: Chongyang
authors.initials: C
authors.lastname: Tang
authors.forename: Meng
authors.initials: M
authors.lastname: Na
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Yifei
authors.initials: Y
authors.lastname: Gu
authors.forename: Guizhen
authors.initials: G
authors.lastname: Ma
authors.forename: Haitao
authors.initials: H
authors.lastname: Ge
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Lin
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018390:Gene Targeting / Q000379:methods; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C583557:IGF1 protein, human; C000597236:IGF1R protein, human; C574206:MIRN422 microRNA, human; D035683:MicroRNAs; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1