title: The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals.
abstract: Glioblastoma is a kind of malignant gliomas that is almost impossible to cure due to the poor drug transportation across the blood-brain barrier and the existence of glioma stem cells. We prepared a new kind of targeted liposomes in order to improve the drug delivery system onto the glioma cells and induce the apoptosis of glioma stem cells afterward. In this experiment, curcumin was chosen to kill gliomas, while quinacrine was used to induce apoptosis of the glioma stem cells. Also,p-aminophenyl-α-D-mannopyranoside could facilitate the transport of liposomes across the blood-brain barrier and finally target the brain glioma cells. The cell experiments in vitro indicated that the targeted liposomes could significantly improve the anti-tumor effects of the drugs, while enhancing the uptake effects, apoptosis effects, and endocytic effects of C6 glioma cells and C6 glioma stem cells. Given the animal experiments in vivo, we discovered that the targeted liposomes could obviously increase the survival period of brain glioma-bearing mice and inhibit the growth of gliomas. In summary, curcumin and quinacrine liposomes modified withp-aminophenyl-α-D-mannopyranoside is a potential preparation to treat brain glioma cells and brain glioma stem cells.
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources and Modernization of TCM, School of Pharmaceutical Sciences, Shihezi University, Shihezi, Xinjiang, People's Republic of China.
authors.forename: Yahua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources and Modernization of TCM, School of Pharmaceutical Sciences, Shihezi University, Shihezi, Xinjiang, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ying
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources and Modernization of TCM, School of Pharmaceutical Sciences, Shihezi University, Shihezi, Xinjiang, People's Republic of China.
authors.forename: Haolun
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources and Modernization of TCM, School of Pharmaceutical Sciences, Shihezi University, Shihezi, Xinjiang, People's Republic of China.
authors.forename: Helu
authors.initials: H
authors.lastname: Yan
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources and Modernization of TCM, School of Pharmaceutical Sciences, Shihezi University, Shihezi, Xinjiang, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources and Modernization of TCM, School of Pharmaceutical Sciences, Shihezi University, Shihezi, Xinjiang, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000166:cytology / Q000187:drug effects; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology*; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D016503:Drug Delivery Systems; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy / Q000473:pathology*; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology*; D051381:Rats
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D003474:Curcumin
keywords: C6 glioblastoma stem cells; apoptosis; blood–brain barrier; brain glioma-bearing rats; curcumin liposomes