title: Compensation of pulsation artifacts during optical imaging with and without cranial chamber.
abstract: Functional Optical Imaging (OI) through the opened skull forms a group of Neuroimaging techniques characterized by a high temporal and spatial resolution on a meso-to macroscopic scale. State of the art OI experiments are generally difficult to execute, with a very timely surgical preparation preceding the experiment, that requires a skilled surgeon to mount a sealed imaging chamber onto the skull. The chamber reduces brain pulsation artifacts and swelling of the brain through movement restriction. In this work, we present preliminary results of a novel approach that does not rely on the usage of an imaging chamber with the goal to facilitate heavily the surgical animal preparation and to allow straightforward joint Electroencephalography - Optical Imaging recordings in the future. We carried out experiments to compare the movement restricting properties of the imaging chamber with the movement in a recording of an unconstrained and periodically irrigated brain. We used high-level image processing techniques to reduce brain pulsation artifacts and did a quantitative movement analysis of the recordings. Our results suggest that while recordings with imaging chamber show less sagittal movement, both with and without imaging chamber comprise the same lateral movements.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Romero-Santiago
authors.forename: Philipp
authors.initials: P
authors.lastname: Flotho
authors.forename: Karsten
authors.initials: K
authors.lastname: Schwerdtfeger
authors.forename: Jacek
authors.initials: J
authors.lastname: Szczygielski
authors.forename: Matthias
authors.initials: M
authors.lastname: Hulser
authors.forename: Lars
authors.initials: L
authors.lastname: Haab
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Strauss
mesh_terms: D000818:Animals; D016477:Artifacts; D001921:Brain / Q000033:anatomy & histology / Q000000981:diagnostic imaging*; D004569:Electroencephalography; D006339:Heart Rate; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009068:Movement; D059906:Neuroimaging / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D051381:Rats; D012886:Skull