title: Lnc-mg is a long non-coding RNA that promotes myogenesis.
abstract: Recent studies indicate important roles for long noncoding RNAs (lncRNAs) as essential regulators of myogenesis and adult skeletal muscle regeneration. However, the specific roles of lncRNAs in myogenic differentiation of adult skeletal muscle stem cells and myogenesis are still largely unknown. Here we identify a lncRNA that is specifically enriched in skeletal muscle (myogenesis-associated lncRNA, in short, lnc-mg). In mice, conditional knockout of lnc-mg in skeletal muscle results in muscle atrophy and the loss of muscular endurance during exercise. Alternatively, skeletal muscle-specific overexpression of lnc-mg promotes muscle hypertrophy. In vitro analysis of primary skeletal muscle cells shows that lnc-mg increases gradually during myogenic differentiation and its overexpression improves cell differentiation. Mechanistically, lnc-mg promotes myogenesis, by functioning as a competing endogenous RNA (ceRNA) for microRNA-125b to control protein abundance of insulin-like growth factor 2. These findings identify lnc-mg as a novel noncoding regulator for muscle cell differentiation and skeletal muscle development.
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.|State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094, China.|Preclinical Medical School, Beijing University of Chinese Medicine, Beijing 100019, China.
authors.forename: Mu
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.
authors.forename: Jiafan
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Discipline of Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen 518116, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China.
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Cai
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Shen
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Preclinical Medical School, Beijing University of Chinese Medicine, Beijing 100019, China.
authors.forename: Sumin
authors.initials: S
authors.lastname: Hu
authors.affiliation: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China.
authors.forename: Zuolin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.
authors.forename: An
authors.initials: A
authors.lastname: Hong
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094, China.
authors.forename: Yingxian
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Guangdong Provincial Key Laboratory of Bioengineering Medicine &National Engineering Research Center of Genetic Medicine, Department of Cell Biology and Institute of Biomedicine, Jinan University, Huang-Pu Avenue West 601, Guangzhou 510632, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D005260:Female; D005786:Gene Expression Regulation; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D024510:Muscle Development / Q000235:genetics*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D009133:Muscular Atrophy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010805:Physical Conditioning, Animal; D061251:Primary Cell Culture; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; C513121:IGF2 protein, mouse; D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse; D062085:RNA, Long Noncoding; D007335:Insulin-Like Growth Factor II