title: Ovar-MHC Polymorphism in Malpura and Avikalin Sheep Vaccinated for Peste des Petits Ruminants (PPR) Virus.
abstract: India harbors a vast diversity of sheep (40 breeds). The study was carried out to assess the genetic diversity of DRB1 and DQA2 locus of the ovar-MHC and their possible association with Peste des petits ruminants (PPR) virus vaccine response in Malpura and Avikalin sheep breeds maintained at an organized institute flock in the semi-arid region of India. Genetic analysis revealed the rich diversity of DRB1 locus with 23 alleles in Malpura and 21 alleles in Avikalin sheep that included 9 new alleles. DQA2 locus also had rich diversity with 19 alleles in Malpura and 20 alleles in Avikalin sheep that included 7 new alleles. At the protein level, high variability alike at the nucleotide level was observed. A marker for footrot susceptibility, DQA2*1101 was absent in both breeds. Genotypic association of DRB1 and DQA2 with PPR vaccine response was statistically non-significant. Vaccine response being a multifactorial (polygenic and influenced by environment) variable, could not show statistically significant association with MHC genotypes in the present study. However, rich genetic diversity of DRB1 and DQA2 gene reflects the importance of this locus for future selection programs.
authors.affiliation: a ICAR-Central Sheep & Wool Research Institute , Avikanagar via Jaipur Rajasthan , India.
authors.forename: G R
authors.initials: GR
authors.lastname: Gowane
authors.affiliation: a ICAR-Central Sheep & Wool Research Institute , Avikanagar via Jaipur Rajasthan , India.
authors.forename: Najif
authors.initials: N
authors.lastname: Akram
authors.affiliation: a ICAR-Central Sheep & Wool Research Institute , Avikanagar via Jaipur Rajasthan , India.
authors.forename: L L L
authors.initials: LLL
authors.lastname: Prince
authors.affiliation: a ICAR-Central Sheep & Wool Research Institute , Avikanagar via Jaipur Rajasthan , India.
authors.forename: Ved
authors.initials: V
authors.lastname: Prakash
authors.affiliation: a ICAR-Central Sheep & Wool Research Institute , Avikanagar via Jaipur Rajasthan , India.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000818:Animals; D014644:Genetic Variation / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D018107:Peste-des-petits-ruminants virus / Q000276:immunology*; D012756:Sheep / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000949:Histocompatibility Antigens Class II; D014765:Viral Vaccines
keywords: Avikalin sheep; DQA2; DRB1; Malpura sheep; PPRV vaccine; environment