title: Formulation for Oral Delivery of Lactoferrin Based on Bovine Serum Albumin and Tannic Acid Multilayer Microcapsules.
abstract: Lactoferrin (Lf) has considerable potential as a functional ingredient in food, cosmetic and pharmaceutical applications. However, the bioavailability of Lf is limited as it is susceptible to digestive enzymes in gastrointestinal tract. The shells comprising alternate layers of bovine serum albumin (BSA) and tannic acid (TA) were tested as Lf encapsulation system for oral administration. Lf absorption by freshly prepared porous 3 μm CaCO3particles followed by Layer-by-Layer assembly of the BSA-TA shells and dissolution of the CaCO3cores was suggested as the most efficient and harmless Lf loading method. The microcapsules showed high stability in gastric conditions and effectively protected encapsulated proteins from digestion. Protective efficiency was found to be 76 ± 6% and 85 ± 2%, for (BSA-TA)4and (BSA-TA)8shells, respectively. The transit of Lf along the gastrointestinal tract (GIT) of mice was followed in vivo and ex vivo using NIR luminescence. We have demonstrated that microcapsules released Lf in small intestine allowing 6.5 times higher concentration than in control group dosed with the same amount of free Lf. Significant amounts of Lf released from microcapsules were then absorbed into bloodstream and accumulated in liver. Suggested encapsulation system has a great potential for functional foods providing lactoferrin.
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore.|Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
authors.forename: Ece
authors.initials: E
authors.lastname: Kilic
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore.|N.G. Chernyshevsky Saratov State University, 83 Astrakhanskaya Street, Saratov, 410012, Russia.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Novoselova
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore.
authors.forename: Su Hui
authors.initials: SH
authors.lastname: Lim
authors.affiliation: National Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk, Republic of Mordovia, 430005, Russia.
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Pyataev
authors.affiliation: National Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk, Republic of Mordovia, 430005, Russia.
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Pinyaev
authors.affiliation: National Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk, Republic of Mordovia, 430005, Russia.
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Kulikov
authors.affiliation: N.G. Chernyshevsky Saratov State University, 83 Astrakhanskaya Street, Saratov, 410012, Russia.|School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, United Kingdom.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Sindeeva
authors.affiliation: N.G. Chernyshevsky Saratov State University, 83 Astrakhanskaya Street, Saratov, 410012, Russia.
authors.forename: Oksana A
authors.initials: OA
authors.lastname: Mayorova
authors.affiliation: AgResearch Limited, Ruakura Research Centre, Bisley Road, Private Bag 3123, Hamilton 3240, New Zealand.
authors.forename: Regan
authors.initials: R
authors.lastname: Murney
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore.
authors.forename: Maria N
authors.initials: MN
authors.lastname: Antipina
authors.affiliation: AgResearch Limited, Ruakura Research Centre, Bisley Road, Private Bag 3123, Hamilton 3240, New Zealand.
authors.forename: Brendan
authors.initials: B
authors.lastname: Haigh
authors.affiliation: School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, United Kingdom.
authors.forename: Gleb B
authors.initials: GB
authors.lastname: Sukhorukov
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore.
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Kiryukhin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002214:Capsules; D002417:Cattle; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D007781:Lactoferrin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D013634:Tannins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D002214:Capsules; D013634:Tannins; D012710:Serum Albumin, Bovine; D007781:Lactoferrin