title: New approaches to antibiotic discovery.
abstract: New antibiotics are urgently required by human medicine as pathogens emerge with developed resistance to almost all antibiotic classes. Pioneering approaches, methodologies and technologies have facilitated a new era in antimicrobial discovery. Innovative culturing techniques such as iChip and co-culturing methods which use 'helper' strains to produce bioactive molecules have had notable success. Exploiting antibiotic resistance to identify antibacterial producers performed in tandem with diagnostic PCR based identification approaches has identified novel candidates. Employing powerful metagenomic mining and metabolomic tools has identified the antibiotic'ome, highlighting new antibiotics from underexplored environments and silent gene clusters enabling researchers to mine for scaffolds with both a novel mechanism of action and also few clinically established resistance determinants. Modern biotechnological approaches are delivering but will require support from government initiatives together with changes in regulation to pave the way for valuable, efficacious, highly targeted, pathogen specific antimicrobial therapies.
authors.affiliation: Department of Life and Physical Sciences, Faculty of Science & Health, Athlone Institute of Technology, Athlone, Ireland.
authors.forename: C
authors.initials: C
authors.lastname: Kealey
authors.affiliation: Department of Life and Physical Sciences, Faculty of Science & Health, Athlone Institute of Technology, Athlone, Ireland.
authors.forename: C A
authors.initials: CA
authors.lastname: Creaven
authors.affiliation: School Of Biomolecular & Biomedical Science, Science Centre - South, Belfield, Dublin 4, Ireland.
authors.forename: C D
authors.initials: CD
authors.lastname: Murphy
authors.affiliation: Department of Life and Physical Sciences, Faculty of Science & Health, Athlone Institute of Technology, Athlone, Ireland. Dbrady@ait.ie.
authors.forename: C B
authors.initials: CB
authors.lastname: Brady
mesh_terms: D000900:Anti-Bacterial Agents; D001230:Aspergillus; D001709:Biotechnology; D018920:Coculture Techniques; D055808:Drug Discovery; D056186:Metagenomics; D013302:Streptomyces
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic; Co-cultivation; Endophyte; In-situ cultivation; Metagenomics