title: Dynamics of 8G12 competitive antibody in "prime-boost" vaccination of Hepatitis E vaccine.
abstract: Hepatitis E virus still poses a great threat to public health worldwide. To date, Hecolin® is the only licensed HEV vaccine in China. Total anti-HEV antibody has been used to reflect vaccine induced immune response in clinical trials for the lack of robust HEV neutralizing antibody detection methods. In this study, we applied a broad neutralizing mouse monoclonal antibody 8G12 to develop a competitive ELSIA assay and quantified 8G12 competitive antibody (8G12-like antibody) in serum samples. The presence of 8G12-like antibody was detected both from participants from HEV vaccine clinical trial and mice immunized with HEV vaccine. Furthermore, 8G12-like antibody was found to have a similar dynamic pattern as anti-HEV antibody during "prime-boost" vaccination, and the proportion of 8G12-like antibody in anti-HEV antibody increased along boost vaccination. Together with previously reported finding that 8G12 could block the most binding of HEV vaccine induced serum antibody to vaccine antigen, we proposed that 8G12-like antibody might be a promising surrogate for vaccine induced HEV neutralizing antibody and had potential to be used as a convenient indicator for HEV vaccine potency evaluation.
authors.affiliation: a National Institutes for Food and Drug Control , Beijing , China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wu
authors.affiliation: a National Institutes for Food and Drug Control , Beijing , China.
authors.forename: Pan
authors.initials: P
authors.lastname: Chen
authors.affiliation: b R&D Department , Shanghai Institute of Biological Products Co., Ltd. , Shanghai , China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Lin
authors.affiliation: c Changchun Institute of Biological Products Co. Ltd., China National Biotech Corporation , Changchun , China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Su
authors.affiliation: a National Institutes for Food and Drug Control , Beijing , China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Hao
authors.affiliation: c Changchun Institute of Biological Products Co. Ltd., China National Biotech Corporation , Changchun , China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cao
authors.affiliation: c Changchun Institute of Biological Products Co. Ltd., China National Biotech Corporation , Changchun , China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: d Jiangsu Provincial Center for Disease Prevention and Control , Nanjing , China.
authors.forename: Fengcai
authors.initials: F
authors.lastname: Zhu
authors.affiliation: a National Institutes for Food and Drug Control , Beijing , China.
authors.forename: Zhenglun
authors.initials: Z
authors.lastname: Liang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D016752:Hepatitis E virus / Q000276:immunology*; D006801:Humans; D007115:Immunization Schedule; D008807:Mice, Inbred BALB C; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines; C577000:hecolin
keywords: 8G12; Hecolin®; Hepatitis E virus; neutralizing antibody; vaccine