title: Clinically compatible flexible wide-field multi-color fluorescence endoscopy with a porcine colon model.
abstract: Early detection of structural or molecular changes in dysplastic epithelial tissues is crucial for cancer screening and surveillance. Multi-targeting molecular endoscopic fluorescence imaging may improve noninvasive detection of precancerous lesions in the colon. Here, we report the first clinically compatible, wide-field-of-view, multi-color fluorescence endoscopy with a leached fiber bundle scope using a porcine model. A porcine colon model that resembles the human colon is used for the detection of surrogate tumors composed of multiple biocompatible fluorophores (FITC, ICG, and heavy metal-free quantum dots (hfQDs)). With anex vivoporcine colon tumor model, molecular imaging with hfQDs conjugated with MMP14 antibody was achieved by spraying molecular probes on a mucosa layer that contains xenograft tumors. With anin vivoporcine colon embedded with surrogate tumors, target-to-background ratios of 3.36 ± 0.43, 2.70 ± 0.72, and 2.10 ± 0.13 were achieved for FITC, ICG, and hfQD probes, respectively. This promising endoscopic technology with molecular contrast shows the capacity to reveal hidden tumors and guide treatment strategy decisions.
authors.affiliation: School of Mechanical Engineering, Gwangju Institute of Science and Technology, Gwangju, South Korea.
authors.forename: Gyugnseok
authors.initials: G
authors.lastname: Oh
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Youngrong
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Biomedical Science and Engineering, Institute of Integrated Technology (IIT), Gwangju Institute of Science and Technology, Gwangju, South Korea.
authors.forename: Su Woong
authors.initials: SW
authors.lastname: Yoo
authors.affiliation: Department of Biomedical Science and Engineering, Institute of Integrated Technology (IIT), Gwangju Institute of Science and Technology, Gwangju, South Korea.
authors.forename: Soonjoo
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Hyunjoo In-Tech, Suite 1901, Daeryung Poster Tower 1, Seoul, South Korea.
authors.forename: Alexey V Dan
authors.initials: AV
authors.lastname: Chin-Yu
authors.affiliation: Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea.
authors.forename: Yeon-Mi
authors.initials: YM
authors.lastname: Ryu
authors.affiliation: Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea; Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Sang-Yeob
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Do
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Sungjee
authors.initials: S
authors.lastname: Kim
authors.affiliation: Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea; Department of Gastroenterology and Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Myung
authors.affiliation: School of Mechanical Engineering, Gwangju Institute of Science and Technology, Gwangju, South Korea; Department of Biomedical Science and Engineering, Institute of Integrated Technology (IIT), Gwangju Institute of Science and Technology, Gwangju, South Korea.
authors.forename: Euiheon
authors.initials: E
authors.lastname: Chung
keywords: (110.0110) Imaging systems; (170.2150) Endoscopic imaging; (170.4580) Optical diagnostics for medicine