title: [Exogenous Bacillus pumilus RNase (binase) suppresses the reproduction of reovirus serotype 1].
abstract: The experimental study identified the antiviral activity of Bacillus pumilus RNase (binase) against the reovirus of serotype 1/strain Lang. For the first time, it has been found that 50 μg/mL of binase effectively reduced the hemagglutinin and cytocidal activity of reovirus in Vero cell line. The preincubation of the enzyme with reovirus before infection of the cells inhibited the viral replication. To determine the stagedependent effect of reovirus reproduction upon binase inhibition, the infected cells were treated with binase or RNase A at different phases of the infectious cycle. The treatment of virus-infected cells has revealed that both enzymes have a maximal antiviral effect on the reovirus propagation during early phases of the reovirus reproduction cycle, with binase being more effective than RNase A. It has been hypothesized that the combined action of the oncolytic reovirus and binase is promising for the elimination of tumor cells carrying mutated RAS gene.
authors.affiliation: Federal Center for Toxicological, Radiation and Biological Safety, Kazan, 420075 Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Efimova
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420008 Russia.|raihan.shah@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Shah Mahmud
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420008 Russia.
authors.forename: P V
authors.initials: PV
authors.lastname: Zelenikhin
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420008 Russia.
authors.forename: M I
authors.initials: MI
authors.lastname: Sabirova
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420008 Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Kolpakov
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420008 Russia.
authors.forename: O N
authors.initials: ON
authors.lastname: Ilinskaya
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002522:Chlorocebus aethiops; D004722:Endoribonucleases / Q000494:pharmacology*; D030102:Orthoreovirus, Mammalian / Q000187:drug effects / Q000502:physiology*; D012260:Ribonucleases / Q000494:pharmacology*; D065288:Serogroup; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D000998:Antiviral Agents; D004722:Endoribonucleases; D012260:Ribonucleases; C022543:ribonuclease T(2); C091047:Bacillus pumilus ribonuclease
keywords: Bacillus pumilus RNase; antiviral agent; binase; reovirus