title: Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue.
abstract: During the last decades, increasing evidence indicated that subcellular organelles do not exist as autarkic units but instead communicate constantly and extensively with each other in various ways. Some communication, for example, the exchange of small molecules, requires the marked convergence of two distinct organelles for a certain period of time. The cross talk between endoplasmic reticulum (ER) and mitochondria, two subcellular organelles of utmost importance for cellular bioenergetics and protein homeostasis, has been increasingly investigated under the last years. This development was significantly driven by the establishment of optimized subcellular fractionation techniques. In this chapter, we will describe and critically discuss the currently used protocol for the isolation of the membrane fraction containing mitochondria-associated membranes (MAM).
authors.affiliation: Center for Alzheimer Research, Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society,, Karolinska Institutet, SE, -141 57, Huddinge, Sweden. bernadette_schreiner@gmx.de.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Schreiner
authors.affiliation: Center for Alzheimer Research, Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society,, Karolinska Institutet, SE, -141 57, Huddinge, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Ankarcrona
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D007425:Intracellular Membranes; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes
keywords: Contact sites; ER; MAM; Mitochondria; Mouse brain; Subcellular fractionation