title: SERS- and luminescence-active Au-Au-UCNP trimers for attomolar detection of two cancer biomarkers.
abstract: Alpha-fetoprotein (AFP) and mucoprotein1 (mucin-1) are two important disease biomarkers. Self-assembled gold nanoparticles (AuNPs) and upconversion nanoparticle (Au-Au-UCNP) trimers based on aptamers were developed for the ultrasensitive detection of AFP and mucin-1. The Au-Au-UCNP trimers produced ideal optical signals, with prominent Raman enhancement and fluorescence quenching effects. The surface-enhanced Raman scattering (SERS) intensity decreased in the presence of mucin-1 and the luminescence intensity increased in the presence of AFP. A limit of detection (LOD) of 4.1 aM and a wide linear range of 0.01-10 fM for the detection of mucin-1 were obtained with this SERS-encoded sensing system. Using the luminescence-encoded sensing system, a LOD of 0.059 aM and a wide linear range of 1-100 aM for the detection of AFP were obtained. These LODs are the lowest values reported so far. This approach has the advantage of detecting two disease biomarkers simultaneously.
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. raxray@gmail.com kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Qu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. raxray@gmail.com kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. raxray@gmail.com kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. raxray@gmail.com kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Liqiang
authors.identifier: http://orcid.org/0000-0001-5325-9399
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. raxray@gmail.com kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. raxray@gmail.com kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. raxray@gmail.com kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Chuanlai
authors.identifier: http://orcid.org/0000-0002-5639-7102
authors.initials: C
authors.lastname: Xu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D006046:Gold; D006801:Humans; D049449:Luminescence; D053768:Metal Nanoparticles; D018396:Mucin-1 / Q000032:analysis*; D013059:Spectrum Analysis, Raman; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: C097353:AFP protein, human; D014408:Biomarkers, Tumor; C060192:MUC1 protein, human; D018396:Mucin-1; D000509:alpha-Fetoproteins; D006046:Gold