title: The extraction and characterisation of a polysaccharide from Moniliophthora perniciosa CCMB 0257.
abstract: The alpha-D-glucans are worldwide acknowledged as powerful immune system stimulants found in several sources; however, the fungal-derived sources appear to respond with higher activity. The present study has investigated polysaccharide production in Moniliophthora perniciosa. The dry biomass was subjected to thermal treatment in alkaline solution after fermentation. The biopolymers dissolved in this solution were precipitated after three volumes of absolute ethanol were added to the supernatant. The pure polysaccharide MPS1 was obtained through molecular exclusion chromatography using the Sephacryl S-200 column. The HPLC-RI analysis showed that MPS1 was a glucose homopolysaccharide. Nuclear magnetic resonance (NMR) spectra indicated the α-form as the anomeric carbon configuration in glucose residue. The structure of the polysaccharide was further confirmed as (1→4)-α-D-glucan through the chemical shift of C4. The molecular weight of MPS1 was 31.0 kDa.
authors.affiliation: a Departamento de Saúde, Laboratório de Enzimologia e Tecnologia de Fermentação , Universidade Estadual de Feira de Santana (UEFS) , Brazil.
authors.forename: Gildomar L
authors.initials: GL
authors.lastname: Valasques Junior
authors.affiliation: b Departamento de Química Orgânica, Instituto de Química , Universidade Federal da Bahia, Campus Universitário de Ondina , Salvador , Brasil.
authors.forename: Elisangela F
authors.initials: EF
authors.lastname: Boffo
authors.affiliation: e Departamento de Saúde , Universidade Estadual de Feira de Santana (UEFS) , Bahia , Brasil.
authors.forename: Jener David G
authors.initials: JDG
authors.lastname: Santos
authors.affiliation: d Departamento de Saúde, Laboratório de Bioprospecção Vegetal (LABIV) , Universidade Estadual de Feira de Santana (UEFS) , Brazil.
authors.forename: Hugo Neves
authors.initials: HN
authors.lastname: Brandão
authors.affiliation: c Departamento de Química Geral e Inorgânica , Universidade Federal da Bahia - UFBA , Salvador , Brasil.
authors.forename: Artur J S
authors.initials: AJS
authors.lastname: Mascarenhas
authors.affiliation: c Departamento de Química Geral e Inorgânica , Universidade Federal da Bahia - UFBA , Salvador , Brasil.
authors.forename: Fernanda T
authors.initials: FT
authors.lastname: Cruz
authors.affiliation: a Departamento de Saúde, Laboratório de Enzimologia e Tecnologia de Fermentação , Universidade Estadual de Feira de Santana (UEFS) , Brazil.
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Assis
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods; D005285:Fermentation; D005936:Glucans; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005936:Glucans; D011134:Polysaccharides
keywords: Moniliophthora perniciosa; characterisation; extraction; polysaccharide; purification