title: Function Identification of the Nucleotides in Keycis-Element ofDYSFUNCTIONAL TAPETUM1(DYT1) Promoter.
abstract: As a core regulatory gene of the anther development,DYSFUNCTIONAL TAPETUM1(DYT1) was expressed in tapetum preferentially. Previous study had confirmed that a "CTCC" sequence withinDYT1promoter was indispensable for correctDYT1expression. However, precise analysis on the function of each nucleotide of this sequence still lacks. Here we employed site mutation assay to identify the function roles of the nucleotides. As a result, the "T" and final "C" of "CTCC" were found essential for the temporal and spatial specificity ofDYT1expression, whereas the other two "C" nucleotides exhibited substitutable somewhat. The substitutes of two flanking nucleotides of "CTCC," however, hardly affected the normal promoter function, suggesting that the "CTCC" sequence as a whole did meet the standard of a canonicalcis-element by definition. In addition, it was found that as short as 497 bpDYT1promoter was sufficient for tissue-specific expression, while longer 505 bpDYT1promoter sequence was sufficient for species-specific expression.
authors.affiliation: Lab of Plant Development Biology, School of Life Sciences, Shanghai University Shanghai, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Lab of Plant Development Biology, School of Life Sciences, Shanghai University Shanghai, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Lab of Plant Development Biology, School of Life Sciences, Shanghai University Shanghai, China.
authors.forename: Ruisha
authors.initials: R
authors.lastname: Li
authors.affiliation: Lab of Plant Development Biology, School of Life Sciences, Shanghai University Shanghai, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hong
authors.affiliation: Lab of Plant Development Biology, School of Life Sciences, Shanghai University Shanghai, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Lab of Plant Development Biology, School of Life Sciences, Shanghai University Shanghai, China.
authors.forename: Qunfang
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Lab of Plant Development Biology, School of Life Sciences, Shanghai University Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
keywords: Arabidopsis; DYT1; Through site mutation assay it was found that the “T” and final “C” nucleotides of key cis-element “CTCC” of Arabidopsis tapetum gene DYT1 promoter were irreplaceable for tissue specific gene expression.; cis-element; tapetum; tissue specificity