title: Novel Ratiometric Fluorescent Nanothermometers Based on Fluorophores-Labeled Short Single-Stranded DNA.
abstract: Novel ratiometric fluorescent short single-stranded DNA (ssDNA) nanothermometers (ssDNA FT) were developed using the fluorescence resonance energy transfer (FRET) effect of the ssDNA's end labeled fluorophores. An optimal ssDNA sequence and associated ssDNA FT were determined through combined MD simulation and temperature-related FRET analysis. Their fluorescence properties and thermo-responsivities were analyzed using fluorescence spectra. The influences of ssDNA' sequence length, sequence composition and fluorescent labels for temperature sensing were investigated. Results revealed the prepared, optimized ssDNA FT showed a high average temperature sensitivity of 7.04% °C1-, wide linear response range of 0-100 °C, and excellent stability with various environmental factors. Furthermore, this ssDNA FT was successfully used for intracellular temperature sensing in cancer cells and was used for in vivo thermos-imaging during microwave hyperthermia of tumor tissue. Advantages in size, sensitivity, and stability proved the feasibility of ssDNA FT in nanoscale thermometry applications, and this novel fluorescent thermometry mechanism is of large potential in the development of FTs. This investigation of ssDNA's molecular thermosensitivity could give rise to a new prospective in the nanothermometry field.
authors.affiliation: Department of Chemistry, School of Science, Xi'an Jiaotong University , Xi'an, 710049, China.
authors.forename: Youshen
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University , Xi'an, 710049, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University , Xi'an, 710049, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University , Xi'an, 710049, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University , Shanghai, 200062, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University , Shanghai, 200062, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University , Xi'an, 710049, China.
authors.forename: Daocheng
authors.identifier: http://orcid.org/0000-0002-6183-539X
authors.initials: D
authors.lastname: Wu
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D011446:Prospective Studies; D013696:Temperature
chemical_list: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes
keywords: FRET; MD simulation; fluorescent nanothermometer; ratiometric thermometer; single-stranded DNA