title: Comparative Genomics of the Aeromonadaceae Core Oligosaccharide Biosynthetic Regions.
abstract: Lipopolysaccharides (LPSs) are an integral part of the Gram-negative outer membrane, playing important organizational and structural roles and taking part in the bacterial infection process. InAeromonas hydrophila,piscicola, andsalmonicida, three different genomic regions taking part in the LPS core oligosaccharide (Core-OS) assembly have been identified, although the characterization of these clusters in most aeromonad species is still lacking. Here, we analyse the conservation of these LPS biosynthesis gene clusters in the all the 170 currently publicAeromonasgenomes, including 30 different species, and characterise the structure of a putative common inner Core-OS in theAeromonadaceaefamily. We describe three new genomic organizations for the inner Core-OS genomic regions, which were more evolutionary conserved than the outer Core-OS regions, which presented remarkable variability. We report how the degree of conservation of the genes from the inner and outer Core-OS may be indicative of the taxonomic relationship betweenAeromonasspecies.
authors.affiliation: Department of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain. gabrifc@gmail.com.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Forn-Cuní
authors.affiliation: Department of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain. smerino@ub.edu.
authors.forename: Susana
authors.initials: S
authors.lastname: Merino
authors.affiliation: Department of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain. jtomas@ub.edu.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tomás
mesh_terms: D044044:Aeromonadaceae / Q000235:genetics* / Q000378:metabolism*; D023061:Gene Order; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000737:chemistry; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D009844:Oligosaccharides
keywords: Aeromonas; genomics; inner core oligosaccharide; lipopolysaccharide; outer core oligosaccharide