title: Accuracy and safety verification of ovarian reserve assessment technique for ovarian tissue transplantation using optical coherence tomography in mice ovary.
abstract: Except for histological study, there are currently no suitable techniques available for the detection and identification of primordial follicles in ovary of primary ovarian insufficiency patients who have undetectable AMH levels. Also, the ability to locate and quantify follicles on ovarian cortex strips, without fixation, is valuable for patients who could undergo subsequent successful ovarian tissue transplantation. Although optical coherence tomography (OCT) is a well-established high resolution imaging technique without fixation commonly applied in biomedicine, few reports are available on ovarian tissue imaging. In present study, we established standard OCT follicle images at each developmental stage, including the primordial follicle, and demonstrated the efficacy of OCT to estimate IVF outcome in transplanted mice ovary like ovarian reserve tests. Unfortunately, the current commercial OCT could not be used to accurate follicle count the number of follicles for whole ovary, because the maximum depth of examination was 100 μm. And we demonstrated the safety of OCT examination, it did not affect IVF outcome and birth defect rate, and reproductive ability. Although there is room for improvement, these findings will be first step to bring OCT examination a step closer to clinical application for measuring true ovarian reserve and localizing follicles.
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Seido
authors.initials: S
authors.lastname: Takae
authors.affiliation: Graduate School of Fundamental Science and Technology, Keio University, Minato, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tsukada
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Yorino
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Okamoto
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Tai
authors.initials: T
authors.lastname: Kawahara
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005307:Fertilization in Vitro; D007150:Immunohistochemistry; D051379:Mice; D065851:Ovarian Reserve; D010053:Ovary / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000637:transplantation*; D016649:Primary Ovarian Insufficiency / Q000000981:diagnostic imaging / Q000601:surgery; D041623:Tomography, Optical Coherence / Q000379:methods; D016896:Treatment Outcome