title: HIV-1 IIIB capture from whole blood on magnetic microparticles.
abstract: Viral load quantification is a critical need for HIV management worldwide. However, the diagnostic technologies currently available are too limited by their size and expense to reach many remote and resource-limited populations. Toward the development of techniques which can be leveraged for point-of-care assays, we have investigated affinity capture of whole viruses using magnetic microparticles functionalized with antibodies or proteins targeting components of the HIV envelope. Results show the best performance from T-20, a small peptide employed in antiretroviral pharmacotherapy which targets an HIV envelope protein. This demonstration introduces an interesting alternative to antibodies for future affinity-capture applications in HIV diagnostics.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Damhorst
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Kooiman
authors.forename: Rashid
authors.initials: R
authors.lastname: Bashir
mesh_terms: D015497:HIV-1 / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D059346:Magnets; D008863:Microspheres; D019095:Point-of-Care Systems; D019562:Viral Load; D054299:env Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus