title: Transport of lipid nano-droplets through MDCK epithelial cell monolayer.
abstract: This study aims to investigate the transport of lipid nano-droplets through MDCK epithelial cell monolayer. Nanoemulsions of self-nano-emulsifying drug delivery systems (SNEDDS) labeled with radioactive C18 triglyceride were developed. The effect of droplet size and lipid composition on the transport was investigated. The results showed that the lipid nano-droplet transport through MDCK cell monolayer was as high as 2.5%. The transport of lipid nano-droplets was higher for nanoemulsions of medium chain glycerides than the long chain glycerides. The transport was reduced by more than half when the average lipid nano-droplet size increased from 38nm to 261nm. The droplet size measurement verified the existence of lipid nano-droplets in the receiver chamber only when the nanoemulsions were added to the donor chamber but not when the surfactant or saline solution was added. Cryo-TEM images confirmed the presence of lipid nano-droplets in both donor and receiver chamber at the end of transport study. In conclusion, lipid nano-droplets can be transported through the cell monolayer. This finding may help to further explore the oral and other non-invasive delivery of macromolecules loaded inside SNEDDS.
authors.affiliation: AmbioPharm, Inc., 1024 Dittman Court, North Augusta, SC 29842, USA.
authors.forename: Pulkit
authors.initials: P
authors.lastname: Khatri
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, 8000 Utopia Parkway, Queens, NY 11439, USA. Electronic address: shaoj@stjohns.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Shao
mesh_terms: D000818:Animals; D002470:Cell Survival; D004285:Dogs; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D004655:Emulsions / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology*; D008055:Lipids / Q000737:chemistry* / Q000493:pharmacokinetics*; D061985:Madin Darby Canine Kidney Cells; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D008055:Lipids
keywords: Cryo-TEM; Droplet size; Lipid composition; Lipid nano-droplet; MDCK cell monolayer; Macromolecule; Nanoemulsion; Oral delivery; SNEDDS; Transport