title: Data representing two separate LC-MS methods for detection and quantification of water-soluble and fat-soluble vitamins in tears and blood serum.
abstract: Two separate liquid chromatography (LC)-mass spectrometry (MS) methods were developed for determination and quantification of water-soluble and fat-soluble vitamins in human tear and blood serum samples. The water-soluble vitamin method was originally developed to detect vitamins B1, B2, B3(nicotinamide), B5, B6(pyridoxine), B7, B9and B12while the fat-soluble vitamin method detected vitamins A, D3, 25(OH)D3,E and K1. These methods were then validated with tear and blood serum samples. In this data in brief article, we provide details on the two LC-MS methods development, methods sensitivity, as well as precision and accuracy for determination of vitamins in human tears and blood serum. These methods were then used to determine the vitamin concentrations in infant and parent samples under a clinical study which were reported in "Determination of Water-Soluble and Fat-Soluble Vitamins in Tears and Blood Serum of Infants and Parents by Liquid Chromatography/Mass Spectrometry DOI:10.1016/j.exer.2016.12.007 [1]". This article provides more details on comparison of vitamin concentrations in the samples with the ranges reported in the literature along with the medically accepted normal ranges. The details on concentrations below the limits of detection (LOD) and limits of quantification (LOQ) are also discussed. Vitamin concentrations were also compared and cross-correlated with clinical data and nutritional information. Significant differences and strongly correlated data were reported in [1]. This article provides comprehensive details on the data with slight differences or slight correlations.
authors.affiliation: Department of Chemical Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA.
authors.forename: Maryam
authors.initials: M
authors.lastname: Khaksari
authors.affiliation: Department of Chemistry, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA.
authors.forename: Lynn R
authors.initials: LR
authors.lastname: Mazzoleni
authors.affiliation: Metabolomics Core, BRCF, University of Michigan, 500 South State Street, Ann Arbor, MI 48109, USA.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Ruan
authors.affiliation: Department of Chemistry, University of Michigan, 500 South State Street, Ann Arbor, MI, 48109, USA.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Kennedy
authors.affiliation: Department of Chemical Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA.
authors.forename: Adrienne R
authors.initials: AR
authors.lastname: Minerick
keywords: Blood serum; Fat-soluble vitamin; Infant; LC-MS method; Parent; Tears; Water-soluble vitamin