title: Effects of obesity on IL-33/ST2 system in heart, adipose tissue and liver: study in the experimental model of Zucker rats.
abstract: Suppression of tumorigenicity 2 (ST2) mediates the effect of Interleukin-33 (IL-33). Few data are reported on the relationship between IL-33/ST2 and obesity. We aimed to investigate effects of obesity on IL-33/ST2 system in heart, adipose tissue and liver in a rodent model of obesity. The relationship of cardiac expression of IL-33/ST2 system with natriuretic peptides (NPs) system and inflammatory mediators was also studied. mRNA expression of IL-33/ST2 system was evaluated in cardiac, adipose and hepatic biopsies from obese Zucker rats (O) and controls (CO). Expression levels of sST2 was significantly lower in O rats compared with CO (p<0.05) in all tissues. Besides, the mRNA levels of IL-33 decreased significant in fat of O respect to CO, while, expression levels of ST2L was significantly higher in liver of CO than in O. A strong relationship of IL-33/ST2 with NPs and classical inflammatory mediators was observed in cardiac tissue. Expression of sST2 in cardiac, adipose and liver tissue decreased in O compared with controls, suggesting an involvement for IL-33/ST2 system in molecular mechanisms of obesity. The strong relationships with NP systems and inflammatory mediators could suggest an involvement for IL-33/ST2 in molecular pathways leading to cardiac dysfunction and inflammation associated with obesity.
authors.affiliation: Scuola Superiore Sant'Anna, Pisa, Pisa, Italy.
authors.forename: Rosetta
authors.initials: R
authors.lastname: Ragusa
authors.affiliation: Institute of Clinical Physiology of CNR, Pisa, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Cabiati
authors.affiliation: Institute of Clinical Physiology of CNR, Pisa, Italy.
authors.forename: Maria Angela
authors.initials: MA
authors.lastname: Guzzardi
authors.affiliation: Scuola Superiore Sant'Anna, Pisa, Pisa, Italy; Harvard Department of Stem Cell and Regenerative Biology, Cambridge, MA, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: D'Amico
authors.affiliation: Institute of Clinical Physiology of CNR, Pisa, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Giannessi
authors.affiliation: Institute of Clinical Physiology of CNR, Pisa, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Del Ry
authors.affiliation: Institute of Clinical Physiology of CNR, Pisa, Italy. Electronic address: chiara.caselli@ifc.cnr.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Caselli
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D000067596:Interleukin-33 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009765:Obesity / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011924:Rats, Zucker; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D000067596:Interleukin-33; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; C000589812:ST2 protein, rat
keywords: Inflammation; Interleukin-33; Obesity; Suppression of tumorigenicity 2