title: Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia.
abstract: To examine the mechanism of ocular axial elongation in myopia, guinea pigs (age: 2-3weeks) which either underwent unilateral or bilateral lens-induced myopization (group 1) or which were primarily myopic at baseline (group 2) received unilateral intraocular injections of amphiregulin antibody (doses: 5, 10, or 15μg) three times in intervals of 9days. A third group of emmetropic guinea pigs got intraocular unilateral injections of amphiregulin (doses: 0.25, 0.50 or 1.00ng, respectively). In each group, the contralateral eyes received intraocular injections of Ringer's solution. In intra-animal inter-eye comparison and intra-eye follow-up comparison in groups 1 and 2, the study eyes as compared to the contralateral eyes showed a dose-dependent reduction in axial elongation. In group 3, study eyes and control eyes did not differ significantly in axial elongation. Immunohistochemistry revealed amphiregulin labelling at the retinal pigment epithelium in eyes with lens-induced myopization and Ringer's solution injection, but not in eyes with amphiregulin antibody injection. Intraocular injections of amphiregulin-antibody led to a reduction of lens-induced axial myopic elongation and of the physiological eye enlargement in young guinea pigs. In contrast, intraocularly injected amphiregulin in a dose of ≤1ng did not show a significant effect. Amphiregulin may be one of several essential molecular factors for axial elongation.
authors.affiliation: Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Wen Jun
authors.initials: WJ
authors.lastname: Jiang
authors.affiliation: The Second College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Hui Xin
authors.initials: HX
authors.lastname: Song
authors.affiliation: The Second College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Shao Yu
authors.initials: SY
authors.lastname: Li
authors.affiliation: The First College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.affiliation: Department of Ophthalmology, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Jian Feng
authors.initials: JF
authors.lastname: Wu
authors.affiliation: The Second College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Guo Ping
authors.initials: GP
authors.lastname: Li
authors.affiliation: Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Da Dong
authors.initials: DD
authors.lastname: Guo
authors.affiliation: The Second College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: De Long
authors.initials: L
authors.lastname: Shi
authors.affiliation: Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China. Electronic address: hongshengbi@126.com.
authors.forename: Hong Sheng
authors.initials: HS
authors.lastname: Bi
authors.affiliation: Department of Ophthalmology, Medical Faculty Mannheim of the Ruprecht-Karls-University Heidelberg, Mannheim, Germany. Electronic address: Jost.Jonas@medma.uni-heidelberg.de.
authors.forename: Jost B
authors.initials: JB
authors.lastname: Jonas
mesh_terms: D066258:Amphiregulin / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D006168:Guinea Pigs; D056965:Injections, Intraocular; D009216:Myopia / Q000188:drug therapy / Q000517:prevention & control*; D012160:Retina / Q000187:drug effects / Q000254:growth & development
chemical_list: D066258:Amphiregulin; D000906:Antibodies
keywords: Amphiregulin; Axial elongation; Epithelial growth factor; Experimental myopia; Myopia