title: Effect of Pancreatic Hormones on pro-Atrial Natriuretic Peptide in Humans.
abstract: Plasma concentrations of pro-Atrial natriuretic peptide, proANP, are decreased in obesity and diabetes. Decreased proANP concentrations have also been noted after meal intake, and recently, a glucose-mediated regulation of ANP gene expression was reported. Hence, we evaluated the effects of insulin, glucagon and glucose on plasma proANP in a series of observational and experimental studies. Six healthy men underwent seven days of bed rest. Before and after the bed rest, hyperinsulinemic euglycemic clamps with serial plasma measurements of proANP were performed. Moreover, plasma proANP was quantified in 65 individuals with normal or impaired glucose regulation. Finally, the effects of infusion-induced hyperglucagonemia were examined in ten healthy men. Bed rest decreased insulin sensitivity and plasma proANP. The decrease in proANP was not associated with insulin sensitivity and the peptide concentrations remained constant during euglycemic hyperinsulinemia and hyperglycemic hyperglucagonemia. Impaired glucose regulation was not associated with decreased proANP concentrations. Bed rest per se induces a marked decrease in plasma proANP concentrations whereas insulin resistance and impaired glucose regulation was not associated with lower proANP concentrations. Neither acute hyperinsulinemia nor hyperglucagonemia seems to affect plasma proANP. Our findings thus suggest that decreased plasma proANP concentrations occur late in the development of insulin resistance.
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Nora E
authors.initials: NE
authors.lastname: Zois
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Dijana
authors.initials: D
authors.lastname: Terzic
authors.affiliation: Steno Diabetes Center, Gentofte, Denmark.
authors.forename: Kristine
authors.initials: K
authors.lastname: Færch
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark; Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Plomgaard
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark; Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Jakob S
authors.initials: JS
authors.lastname: Hansen
authors.affiliation: Steno Diabetes Center, Gentofte, Denmark; Faculty of Health, Aarhus University, Aarhus, Denmark; Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Rossing
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark; Faculty of Health, Aarhus University, Aarhus, Denmark; Cardiorenal Research Laboratory, Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA. Electronic address: JPG@dadlnet.dk.
authors.forename: Jens P
authors.initials: JP
authors.lastname: Goetze
mesh_terms: D000328:Adult; D009320:Atrial Natriuretic Factor / Q000097:blood*; D001510:Bed Rest / Q000009:adverse effects*; D001786:Blood Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance; D008297:Male
chemical_list: D001786:Blood Glucose; D007328:Insulin; C552580:midregional pro-atrial natriuretic peptide, human; D009320:Atrial Natriuretic Factor
keywords: Hyperglycemia; Insulin resistance; Metabolism; Prediabetes; ProANP