title: The effects of microRNA-183 promots cell proliferation and invasion by targeting MMP-9 in endometrial cancer.
abstract: MiRNAs are known to play important roles in cancer cell development. However, the pattern and biological role of miR-183 in endometrial cancer (EC) have not been completely unexplored. Here, we found that miR-183 was upregulated in endometrial cancer cells. The purpose of the study was to evaluate the function of miR-183 in the endometrial cancer cell line and the mechanisms regulating its direct target protein in these processes. The mRNA and protein expressions were analyzed by quantitative RT-PCR and western blotting, respectively. The experiments about MTT assay, colony formation assay and transwell assay showed that miR-183 can positively regulate cell proliferation, migration and invasion in vitro. Furthermore, the in vivo experiments indicated that knockdown of miR-183 significantly attenuated EC cells growth. Mechanistically, luciferase reporter assay and western blotting assay was conducted to confirm target associations. The data analysis revealed that MMP-9 as a direct target of miR-183 in EC and there was a negatively relationship between miR-183 and MMP-9 expression in EC cells. Taken together, our results demonstrated that miR-183 plays a critical role in EC tumorigenesis and metastasis by suppressing MMP-9 expression, which may be an attractive therapeutic target for the treatment of endometrial cancer.
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210000, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Ruan
authors.affiliation: Department of Clinical Laboratory, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210000, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Clinical Laboratory, Nanjing Maternity and Child Health Care Hospital, Nanjing 210000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory, Huai'an Hospital, Xuzhou Medical University, Huai'an 223002, China. Electronic address: mal_huaian@sina.com.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Gynecology, Jiangsu Cancer Hospital, Nanjing 210000, China. Electronic address: zhaoybx_2016@sina.com.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D059467:Transcriptome
chemical_list: C533449:MIRN183 microRNA, human; D035683:MicroRNAs; D020780:Matrix Metalloproteinase 9
keywords: Endometrial cancer; Invasion; MMP-9; Migration; Proliferation; miR-183