title: Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharides in intestinal epithelial cells.
abstract: Seleno-polysaccharides possess a variety of biological activities. In the present study, we further investigated the immunomodulatory effects of low molecular-weight seleno-aminopolysaccharides (LSA) in intestinal porcine epithelial cells (IPEC-1), and the molecular mechanisms of these effects. Analysis by ELISAs revealed that LSA could significantly increase the secretion of nitric oxide (NO), interleukin- 6 (IL-6), interleukin- 10 (IL-10), and tumor necrosis factor alpha (TNF-α). Moreover, LSA dramatically increased the gene expression levels of TNF-α, IL-6, IL-10, and iNOS in IPEC-1 cells, as determined by qRT-PCR. Western blot analysis further determined that LSA promotes inhibitor kappa B α (IĸBα), nuclear factor- kappa B (NF-κB) p65 phosphorylation. Taken together, these findings suggested that LSA has immunomodulatory activity on IPEC-1 cells, and its mechanism may be related to activation of the NF-ĸB signaling pathway.
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhejiang 316022, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Gu
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: zswenmr@163.com.
authors.forename: Zheng-Shun
authors.initials: ZS
authors.lastname: Wen
authors.affiliation: Zhejiang Marine Development Research Institute, Zhoushan, Zhejiang 316021, China.
authors.forename: Xing-Wei
authors.initials: XW
authors.lastname: Xiang
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhejiang 316022, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhejiang 316022, China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Wang
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
authors.forename: Jian-Yin
authors.initials: JY
authors.lastname: Ma
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
authors.forename: You-Le
authors.initials: YL
authors.lastname: Qu
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007413:Intestinal Mucosa / Q000166:cytology*; D008970:Molecular Weight; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D013552:Swine
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D016328:NF-kappa B; D011134:Polysaccharides; D012333:RNA, Messenger; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D012643:Selenium
keywords: Immunomodulatory activity; Intestinal epithelial cells; Low molecular-weight seleno-aminopolysaccharide