title: The genetic diversity of chicken breeds from Jiangxi, assessed with BCDO2 and the complete mitochondrial DNA D-loop region.
abstract: The Jiangxi Province of China has numerous native domestic chicken breeds, including some black skin breeds. The genetic diversity of Jiangxi native chickens is largely unknown, and specifically, the genetic contribution of the grey junglefowl to black skin chickens is not well understood. To address these questions, the complete D-loop region of the mitochondrial DNA (mtDNA) and beta-carotene dioxygenase 2(BCDO2)gene was sequenced in a total of 209 chickens representing seven Jiangxi native breeds. Thirty-one polymorphic sites were identified across the complete mtDNA D-loop region sequence. Twenty-three haplotypes were observed in the seven breeds, which belonged to four distinct mitochondrial clades (A, B, C and E). Clade A and B were dominant in the chickens with a frequency of approximately 67.9%. There were five SNPs that defined two haplotypes, W and Y in BCDO2. Four breeds had one haplotype and three breeds had two. We conclude that Jiangxi native chicken breeds have relatively low genetic diversity and likely share four common maternal lineages from two different maternal ancestors of junglefowl. Furthermore, some Jiangxi chicken populations may have been mixed with chickens with exotic lineage. Further research should be established to protect these domestic chicken resources.
authors.affiliation: Jiangsu institute of Poultry Science, Yangzhou, China.
authors.forename: Yu-Shi
authors.initials: YS
authors.lastname: Gao
authors.affiliation: Jiangsu institute of Poultry Science, Yangzhou, China.
authors.forename: Xiao-Xu
authors.identifier: http://orcid.org/0000-0003-4803-9599
authors.initials: XX
authors.lastname: Jia
authors.affiliation: Jiangsu institute of Poultry Science, Yangzhou, China.
authors.forename: Xiu-Jun
authors.initials: XJ
authors.lastname: Tang
authors.affiliation: Jiangsu institute of Poultry Science, Yangzhou, China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Fan
authors.affiliation: Jiangsu institute of Poultry Science, Yangzhou, China.
authors.forename: Jun-Xian
authors.initials: JX
authors.lastname: Lu
authors.affiliation: Jiangsu institute of Poultry Science, Yangzhou, China.
authors.forename: Sheng-Hai
authors.initials: SH
authors.lastname: Huang
authors.affiliation: Jiangsu institute of Poultry Science, Yangzhou, China.
authors.forename: Meng-Jun
authors.initials: MJ
authors.lastname: Tang
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000235:genetics; D030161:Avian Proteins / Q000235:genetics*; D001947:Breeding; D002645:Chickens / Q000235:genetics; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D006239:Haplotypes; D008928:Mitochondria / Q000235:genetics; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D019207:beta Carotene / Q000235:genetics; D050559:beta-Carotene 15,15'-Monooxygenase / Q000235:genetics*
chemical_list: D030161:Avian Proteins; D004272:DNA, Mitochondrial; D019207:beta Carotene; D050559:beta-Carotene 15,15'-Monooxygenase