title: Fluorescent, Magnetic Multifunctional Carbon Dots for Selective Separation, Identification, and Eradication of Drug-Resistant Superbugs.
abstract: The emergence of drug-resistant superbugs remains a major burden to society. As the mortality rate caused by sepsis due to superbugs is more than 40%, accurate identification of blood infections during the early stage will have a huge significance in the clinical setting. Here, we report the synthesis of red/blue fluorescent carbon dot (CD)-attached magnetic nanoparticle-based multicolor multifunctional CD-based nanosystems, which can be used for selective separation and identification of superbugs from infected blood samples. The reported data show that multifunctional fluorescent magneto-CD nanoparticles are capable of isolating Methicillin-resistantStaphylococcus aureus(MRSA) andSalmonellaDT104 superbug from whole blood samples, followed by accurate identification via multicolor fluorescence imaging. As multidrug-resistant (MDR) superbugs are resistant to antibiotics available in the market, this article also reports the design of antimicrobial peptide-conjugated multicolor fluorescent magneto-CDs for effective separation, accurate identification, and complete disinfection of MDR superbugs from infected blood. The reported data demonstrate that by combining pardaxin antimicrobial peptides, magnetic nanoparticles, and multicolor fluorescent CDs into a single system, multifunctional CDs represent a novel material for efficient separation, differentiation, and eradication of superbugs. This material shows great promise for use in clinical settings.
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Avijit
authors.initials: A
authors.lastname: Pramanik
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Stacy
authors.initials: S
authors.lastname: Jones
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio , One UTSA Circle, San Antonio, Texas 78249-0697, United States.
authors.forename: Francisco
authors.initials: F
authors.lastname: Pedraza
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Aruna
authors.initials: A
authors.lastname: Vangara
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Carrie
authors.initials: C
authors.lastname: Sweet
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Mariah S
authors.initials: MS
authors.lastname: Williams
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Vikram
authors.initials: V
authors.lastname: Ruppa-Kasani
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Sean Edward
authors.initials: SE
authors.lastname: Risher
authors.affiliation: Department of Physics and Astronomy, University of Texas at San Antonio , One UTSA Circle, San Antonio, Texas 78249-0697, United States.
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Sardar
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , 1400 J. R. Lynch Street, P.O. Box 17910, Jackson, Mississippi 39217-0510, United States.
authors.forename: Paresh Chandra
authors.initials: PC
authors.lastname: Ray