title: An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins.
abstract: The iminodiacetate-Ni2+-hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization of hexahistidine-tagged (His-tagged) proteins using PPEIDA was demonstrated.
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Cai
authors.forename: Ying
authors.initials: Y
authors.lastname: Tan
authors.forename: Chunyan
authors.initials: C
authors.lastname: Tan
authors.forename: Jiatao
authors.initials: J
authors.lastname: Wu
authors.forename: Pan
authors.initials: P
authors.lastname: Wu
authors.forename: Jiamei
authors.initials: J
authors.lastname: Liang
authors.forename: Shuwen
authors.initials: S
authors.lastname: Liu
authors.forename: Bibo
authors.initials: B
authors.lastname: Zhang
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang