title: Two- and Three-dimensional Mid-Infrared Chemical Imaging.
abstract: This article describes the state-of-the-art in 2D and 3D tomographic mid-IR imaging and its current and potential applications in the imaging of material and biological systems with a focus on cells and tissues. 2D FTIR microscopy is first presented in terms of the basic experimental configurations of the technique, optics, data and image acquisition. This first section provides a basis for the ensuing discussion on 3D FTIR spectro-microtomography. In the second part, examples of 2D and 3D chemical imaging are given with a focus on the use of molecular chemical probes and probe-free applications, respectively.
authors.affiliation: Department of Chemistry, University of Fribourg Chemin du Musée 9, CH-1700 Fribourg;, Email: fabio.zobi@unifr.ch.
authors.forename: Fabio
authors.initials: F
authors.lastname: Zobi
authors.affiliation: BayCEER, University of Bayreuth Dr-Hans-Frisch-Str. 1-3, 95448 Bayreuth, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Obst
mesh_terms: D021621:Imaging, Three-Dimensional; D019697:Onions / Q000166:cytology*; D009930:Organic Chemicals / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000295:instrumentation; D017550:Spectroscopy, Fourier Transform Infrared; D014054:Tomography / Q000295:instrumentation
chemical_list: D009930:Organic Chemicals