title: An innovative application of time-domain spectroscopy on localized surface plasmon resonance sensing.
abstract: White-light scanning interferometry (WLSI) is often used to study the surface profiles and properties of thin films because the strength of the technique lies in its ability to provide fast and high resolution measurements. An innovative attempt is made in this paper to apply WLSI as a time-domain spectroscopic system for localized surface plasmon resonance (LSPR) sensing. A WLSI-based spectrometer is constructed with a breadboard of WLSI in combination with a spectral centroid algorithm for noise reduction and performance improvement. Experimentally, the WLSI-based spectrometer exhibits a limit of detection (LOD) of 1.2 × 10-3refractive index units (RIU), which is better than that obtained with a conventional UV-Vis spectrometer, by resolving the LSPR peak shift. Finally, the bio-applicability of the proposed spectrometer was investigated using the rs242557 tau gene, an Alzheimer's and Parkinson's disease biomarker. The LOD was calculated as 15 pM. These results demonstrate that the proposed WLSI-based spectrometer could become a sensitive time-domain spectroscopic biosensing platform.
authors.affiliation: Thin Film Technology Center/Department of Optics and Photonics, National Central University, Taoyuan 32001, Taiwan.
authors.forename: Meng-Chi
authors.initials: MC
authors.lastname: Li
authors.affiliation: BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Chang
authors.affiliation: Thin Film Technology Center/Department of Optics and Photonics, National Central University, Taoyuan 32001, Taiwan.
authors.forename: Huai-Yi
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Thin Film Technology Center/Department of Optics and Photonics, National Central University, Taoyuan 32001, Taiwan.
authors.forename: Yu-Xen
authors.initials: YX
authors.lastname: Lin
authors.affiliation: Thin Film Technology Center/Department of Optics and Photonics, National Central University, Taoyuan 32001, Taiwan.
authors.forename: Chien-Cheng
authors.initials: CC
authors.lastname: Kuo
authors.affiliation: BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Ja-An
authors.initials: JA
authors.lastname: Annie Ho
authors.affiliation: Thin Film Technology Center/Department of Optics and Photonics, National Central University, Taoyuan 32001, Taiwan.
authors.forename: Cheng-Chung
authors.initials: CC
authors.lastname: Lee
authors.affiliation: Department of Optoelectric Physics, Chinese Culture University, Taipei 11114, Taiwan.
authors.forename: Li-Chen
authors.initials: LC
authors.lastname: Su