title: Fabrication and characterization of chitosan/OGP coated porous poly(ε-caprolactone) scaffold for bone tissue engineering.
abstract: As one of the stimulators on bone formation, osteogenic growth peptide (OGP) improves both proliferation and differentiation of the bone cells in vitro and in vivo. The aim of this work was the preparation of three dimensional porous poly(ε-caprolactone) (PCL) scaffold with high porosity, well interpore connectivity, and then its surface was modified by using chitosan (CS)/OGP coating for application in bone regeneration. In present study, the properties of porous PCL and CS/OGP coated PCL scaffold, including the microstructure, water absorption, porosity, hydrophilicity, mechanical properties, and biocompatibility in vitro were investigated. Results showed that the PCL and CS/OGP-PCL scaffold with an interconnected network structure have a porosity of more than 91.5, 80.8%, respectively. The CS/OGP-PCL scaffold exhibited better hydrophilicity and mechanical properties than that of uncoated PCL scaffold. Moreover, the results of cell culture test showed that CS/OGP coating could stimulate the proliferation and growth of osteoblast cells on CS/OGP-PCL scaffold. These finding suggested that the surface modification could be a effective method on enhancing cell adhesion to synthetic polymer-based scaffolds in tissue engineering application and the developed porous CS/OGP-PCL scaffold should be considered as alternative biomaterials for bone regeneration.
authors.affiliation: a School of Materials Science and Engineering, Fujian University of Technology , Fuzhou , China.|b Fujian Provincial Key Laboratory of Advanced Materials Processing and Application , Fuzhou , China.|c School of Materials Science and Engineering, Fuzhou University , Fuzhou , China.|d National Center for International Research of Micro-nano Molding Technology & Key Laboratory for Micro Molding Technology of Henan Province , Zhengzhou University , Zhengzhou , China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Cui
authors.affiliation: a School of Materials Science and Engineering, Fujian University of Technology , Fuzhou , China.|b Fujian Provincial Key Laboratory of Advanced Materials Processing and Application , Fuzhou , China.
authors.forename: Luyin
authors.initials: L
authors.lastname: Lin
authors.affiliation: a School of Materials Science and Engineering, Fujian University of Technology , Fuzhou , China.|b Fujian Provincial Key Laboratory of Advanced Materials Processing and Application , Fuzhou , China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Si
authors.affiliation: e School of Pharmacy, Fujian Medical University , Fuzhou , China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Luo
authors.affiliation: a School of Materials Science and Engineering, Fujian University of Technology , Fuzhou , China.|b Fujian Provincial Key Laboratory of Advanced Materials Processing and Application , Fuzhou , China.
authors.forename: Qianting
authors.initials: Q
authors.lastname: Wang
authors.affiliation: a School of Materials Science and Engineering, Fujian University of Technology , Fuzhou , China.|b Fujian Provincial Key Laboratory of Advanced Materials Processing and Application , Fuzhou , China.
authors.forename: Yongnan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: d National Center for International Research of Micro-nano Molding Technology & Key Laboratory for Micro Molding Technology of Henan Province , Zhengzhou University , Zhengzhou , China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: a School of Materials Science and Engineering, Fujian University of Technology , Fuzhou , China.|b Fujian Provincial Key Laboratory of Advanced Materials Processing and Application , Fuzhou , China.|c School of Materials Science and Engineering, Fuzhou University , Fuzhou , China.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Chen
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry*; D008422:Materials Testing; D055595:Mechanical Phenomena; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; C074533:osteogenic growth peptide; C016240:polycaprolactone; D048271:Chitosan
keywords: Poly(ε-caprolactone) (PCL); chitosan (CS); coating; osteogenic growth peptide (OGP); porous scaffold