title: Efficient Capture of Cancer Cells by Their Replicated Surfaces Reveals Multiscale Topographic Interactions Coupled with Molecular Recognition.
abstract: Cell-surface topographic interactions can direct the design of biointerfaces, which have been widely used in isolation of circulating tumor cells or fundamental cell biological research. By using three kinds of cancer cell-replicated surfaces with differentiated structures, we uncover that multiscale-cooperative topographic interactions (at both nanoscale and microscale) coupled with molecular recognition enable efficient and specific isolation of cancer cells. The cell replicas precisely inherit the structural features from the original cancer cells, providing not only preferable structures for matching with cancer cells but also a unique platform to interrogate whether certain cancer cells can optimally match with their own replicated surfaces. The results reveal that cancer cells do not show preferential recognitions to their respective replicas, while the capture agent-modified surfaces with hierarchical structures exhibit improved cancer cell capture efficiencies. Two levels of topographic interactions between cancer cells and cell replica surfaces exist. Nanoscale filopodia on cancer cells can topographically interact with different nanostructures on replica surfaces. In addition, microscale concave/convex on surfaces provide suitable sites for trapping cancer cells. This study may promote smart design of multiscale biofunctional materials that can specifically recognize cancer cells.
authors.affiliation: University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Wenshuo
authors.initials: W
authors.lastname: Wang
authors.affiliation: University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Cui
authors.affiliation: University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Zhang
authors.forename: Jingxin
authors.initials: J
authors.lastname: Meng
authors.affiliation: University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Feilong
authors.initials: F
authors.lastname: Zhang
authors.affiliation: University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
authors.forename: Shutao
authors.identifier: http://orcid.org/0000-0002-2559-5181
authors.initials: S
authors.lastname: Wang
mesh_terms: D006801:Humans; D049329:Nanostructures; D009369:Neoplasms; D009360:Neoplastic Cells, Circulating; D013499:Surface Properties
keywords: biointerface; cancer cell recognition; cell mineralization; hierarchical structure; topographic interaction