title: [Development and Evaluation of a New Selective Culture Medium, KBM Anaero RS-GNR, for Detection of Anaerobic Gram Negative Rods].
abstract: The laboratory culture methods for isolating drug-resistant pathogens has been the gold standard in medical microbiology, and play pivotal roles in the overall management of infectious diseases. Recently, several reports have emphasized the development of antibiotics-resistance among anaerobic gram-negative rods, especially GenusBacteroidesandPrevotella. Therefore, a selective culture method to detect these pathogens is needed. We developed here the new selective culture medium, termed "KBM Anaero RS-GNR," for detecting anaerobic Gram-negative rods. Growth capability and selectivity of the agar medium were assessed by using the pure culture suspensions of more than 100 bacterial strains as well as the 13 samples experimentally contaminated with these bacterial strains. This new medium, "KBM Anaero RS-GNR," successfully showed the selective isolation of anaerobic Gram-negative rods. Compared with commercially available medium, "PV Brucella HK Agar, " which is also designed to detect anaerobic Gram-negative rods, there was no significant difference of the overall detection efficiency between two media. However, "KBM Anaero RS-GNR" showed superior to selectivity for anaerobic Gram-negative rods, especially from the samples contaminated withCandidaspecies. Thus, the culture method using KBM Anaero RS-GNR is relevant for isolation of anaerobic Gram-negative rods especially from clinical specimens.
authors.affiliation: Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital.
authors.forename: Taeko
authors.initials: T
authors.lastname: Narita
authors.affiliation: Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital.
authors.forename: Kyohei
authors.initials: K
authors.lastname: Kato
authors.affiliation: Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hanaiwa
authors.affiliation: Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital.
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Harada
authors.affiliation: Department of Medical Technology and Sciences, International University of Health and Welfare.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Funashima
authors.affiliation: Microbiology Research Division, Kohjin Bio Co., Ltd.
authors.forename: Makoto
authors.initials: M
authors.lastname: Akiwa
authors.affiliation: Microbiology Research Division, Kohjin Bio Co., Ltd.
authors.forename: Jun-Ichiro
authors.initials: JI
authors.lastname: Sekiguchi
authors.affiliation: Department of Medical Technology and Sciences, International University of Health and Welfare.
authors.forename: Zenzo
authors.initials: Z
authors.lastname: Nagasawa
authors.affiliation: Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital. Department of Medical Technology and Sciences, International University of Health and Welfare.
authors.forename: Tsukuru
authors.initials: T
authors.lastname: Umemura
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D011817:Rabbits
chemical_list: D003470:Culture Media