title: Pig Induced Pluripotent Stem Cell-Derived Neural Rosettes Parallel Human Differentiation Into Sensory Neural Subtypes.
abstract: The pig is the large animal model of choice for study of nerve regeneration and wound repair. Availability of porcine sensory neural cells would conceptually allow for analogous cell-based peripheral nerve regeneration in porcine injuries of similar severity and size to those found in humans. After recently reporting that porcine (or pig) induced pluripotent stem cells (piPSCs) differentiate into neural rosette (NR) structures similar to human NRs, here we demonstrate that pig NR cells could differentiate into neural crest cells and other peripheral nervous system-relevant cell types. Treatment with either bone morphogenetic protein 4 or fetal bovine serum led to differentiation into BRN3A-positive sensory cells and increased expression of sensory neuron TRK receptor gene family: TRKA, TRKB, and TRKC. Porcine sensory neural cells would allow determination of parallels between human and porcine cells in response to noxious stimuli, analgesics, and reparative mechanisms. In vitro differentiation of pig sensory neurons provides a novel model system for neural cell subtype specification and would provide a novel platform for the study of regenerative therapeutics by elucidating the requirements for innervation following injury and axonal survival.
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
authors.forename: Robin L
authors.initials: RL
authors.lastname: Webb
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
authors.forename: Amalia
authors.initials: A
authors.lastname: Gallegos-Cárdenas
authors.affiliation: 2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
authors.forename: Colette N
authors.initials: CN
authors.lastname: Miller
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Solomotis
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Liu
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
authors.forename: Franklin D
authors.initials: FD
authors.lastname: West
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
authors.forename: Steven L
authors.initials: SL
authors.lastname: Stice
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D058953:Neural Stem Cells / Q000166:cytology* / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D012397:Rosette Formation; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000502:physiology; D013552:Swine
chemical_list: D015415:Biomarkers
keywords: neural crest cells; neural development; sensory neural cells