title: Preclinical Mammalian Safety Studies of EPHARNA (DOPC Nanoliposomal EphA2-Targeted siRNA).
abstract: To address the need for efficient and biocompatible delivery systems for systemic siRNA delivery, we developed 1,2-Dioleoyl-sn-Glycero-3-Phosphatidylcholine (DOPC) nanoliposomal EphA2-targeted therapeutic (EPHARNA). Here, we performed safety studies of EPHARNA in murine and primate models. Single dosing of EPHARNA was tested at 5 concentrations in mice (N= 15 per group) and groups were sacrificed on days 1, 14, and 28 for evaluation of clinical pathology and organ toxicity. Multiple dosing of EPHARNA was tested in mice andRhesusmacaques twice weekly at two dose levels in each model. Possible effects on hematologic parameters, serum chemistry, coagulation, and organ toxicity were assessed. Following single-dose EPHARNA administration to mice, no gross pathologic or dose-related microscopic findings were observed in either the acute (24 hours) or recovery (14 and 28 days) phases. The no-observed-adverse-effect level (NOAEL) for EPHARNA is considered >225 μg/kg when administered as a single injection intravenously in CD-1 mice. With twice weekly injection, EPHARNA appeared to stimulate a mild to moderate inflammatory response in a dose-related fashion. There appeared to be a mild hemolytic reaction in the female mice. InRhesusmacaques, minimal to moderate infiltration of mononuclear cells was found in some organs including the gastrointestinal tract, heart, and kidney. No differences attributed to EPHARNA were observed. These results demonstrate that EPHARNA is well tolerated at all doses tested. These data, combined with previously publishedin vivovalidation studies, have led to an ongoing first-in-human phase I clinical trial (NCT01591356).Mol Cancer Ther; 16(6); 1114-23. ©2017 AACR.
authors.affiliation: Division of Cancer Medicine, MD Anderson Cancer Center, Houston, Texas.|Department of Gynecologic Oncology, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Wagner
authors.affiliation: Department of Gynecologic Oncology, MD Anderson Cancer Center, Houston, Texas.|Center for RNA Interference and Non-Coding RNA, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Rahul
authors.initials: R
authors.lastname: Mitra
authors.affiliation: Department of Veterinary Medicine and Surgery, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: McArthur
authors.affiliation: Department of Veterinary Medicine and Surgery, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Wallace
authors.initials: W
authors.lastname: Baze
authors.affiliation: Department of Veterinary Medicine and Surgery, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Kirstin
authors.initials: K
authors.lastname: Barnhart
authors.affiliation: Department of Gynecologic Oncology, MD Anderson Cancer Center, Houston, Texas.|Center for RNA Interference and Non-Coding RNA, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Sherry Y
authors.initials: SY
authors.lastname: Wu
authors.affiliation: Center for RNA Interference and Non-Coding RNA, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Cristian
authors.initials: C
authors.lastname: Rodriguez-Aguayo
authors.affiliation: Center for RNA Interference and Non-Coding RNA, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Xinna
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Gynecologic Oncology, MD Anderson Cancer Center, Houston, Texas.|Center for RNA Interference and Non-Coding RNA, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Coleman
authors.affiliation: Center for RNA Interference and Non-Coding RNA, MD Anderson Cancer Center, Houston, Texas. asood@mdanderson.org glopez@mdanderson.org.|Department of Experimental Therapeutics, MD Anderson Cancer Center, Houston Texas.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Lopez-Berestein
authors.affiliation: Department of Gynecologic Oncology, MD Anderson Cancer Center, Houston, Texas. asood@mdanderson.org glopez@mdanderson.org.|Center for RNA Interference and Non-Coding RNA, MD Anderson Cancer Center, Houston, Texas.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sood
mesh_terms: D000818:Animals; D015415:Biomarkers; D004353:Drug Evaluation, Preclinical; D005260:Female; D006801:Humans; D008081:Liposomes; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D053758:Nanoparticles; D010713:Phosphatidylcholines; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics* / Q000493:pharmacokinetics; D036104:Receptor, EphA2 / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D015415:Biomarkers; D008081:Liposomes; D010713:Phosphatidylcholines; D034741:RNA, Small Interfering; D036104:Receptor, EphA2; C017251:1,2-oleoylphosphatidylcholine