title: Imaging Vesicular Traffic at the Immune Synapse.
abstract: Immunological synapse formation is the result of a profound T cell polarization process that involves the coordinated action of the actin and microtubule cytoskeleton, as well as intracellular vesicle traffic. Endosomal vesicle traffic ensures the targeting of the T cell receptor (TCR) and various signaling molecules to the synapse, being necessary for the generation of signaling complexes downstream of the TCR. Here we describe the microscopy imaging methods that we currently use to unveil how TCR and signaling molecules are associated with endosomal compartments and deliver their cargo to the immunological synapse.
authors.affiliation: Department of Immunology, Lymphocyte Cell Biology Unit, Institut Pasteur, 25-28 Rue du Docteur Roux, Paris, 75015, France.|INSERM U-1221, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bouchet
authors.affiliation: Department of Immunology, Lymphocyte Cell Biology Unit, Institut Pasteur, 25-28 Rue du Docteur Roux, Paris, 75015, France.|INSERM U-1221, Paris, France.
authors.forename: Iratxe
authors.initials: I
authors.lastname: Del Río-Iñiguez
authors.affiliation: Department of Immunology, Lymphocyte Cell Biology Unit, Institut Pasteur, 25-28 Rue du Docteur Roux, Paris, 75015, France. andres.alcover@pasteur.fr.|INSERM U-1221, Paris, France. andres.alcover@pasteur.fr.
authors.forename: Andrés
authors.initials: A
authors.lastname: Alcover
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000276:immunology; D022162:Cytoplasmic Vesicles / Q000276:immunology*; D011992:Endosomes / Q000276:immunology*; D006801:Humans; D054992:Immunological Synapses / Q000276:immunology*; D019169:Jurkat Cells; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: Cytoskeleton; Endosomes; Immunological synapse; T cell activation; TCR signaling; Vesicle traffic