title: Influencing Selectivity to Cancer Cells with Mixed Nanoparticles Prepared from Albumin-Polymer Conjugates and Block Copolymers.
abstract: Albumin-based nanoparticles are widely used to delivery anticancer drug because they promote the accumulation of drugs in tumor sites. Nanoparticles with surface immobilized albumin are widely described in literature, although mixed nanoparticles with systematically modified ratios between albumin and PEG-based material are less common. In this work, hybrid nanoparticles were prepared by coassembly of a PEG-based amphiphilic block copolymer together with a polymer-protein conjugate. Poly(oligo(ethylene glycol) methyl ether acrylate)-poly(ε-caprolactone) (POEGMEA-PCL) was prepared by a combination of ring-opening polymerization and reversible addition-fragmentation chain transfer (RAFT) polymerization, while the polymer-protein conjugate was obtained by reacting poly(ε-caprolactone) with bovine serum albumin (BSA-PCL). Co-assembly of both amphiphiles at different ratios, with and without curcumin as a drug, led to hybrid nanoparticles with various amount of albumin on the particle surface. The resulting hybrid nanoparticles were similar in size (100-120 nm), but increasing the amount of albumin on the surface led to a more-negative ζ potential. The cytotoxicity of the curcumin-loaded nanoparticles was examined on several cell lines. The curcumin-loaded nanoparticles with high amount of albumin led to high cytotoxicity against breast cancer cell lines (MDA-MB-231 and MCF-7), which coincided with high cellular uptake. However, the cytotoxicity of the curcumin-loaded nanoparticles against CHO cells and RAW264.7 cells was reduced, suggesting that albumin can facilitate selectivity toward cancer cells.
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Kensington NSW 2052, Australia.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Kensington NSW 2052, Australia.
authors.forename: Sandy
authors.initials: S
authors.lastname: Wong
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Kensington NSW 2052, Australia.
authors.forename: Fan
authors.initials: F
authors.lastname: Chen
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Kensington NSW 2052, Australia.
authors.forename: Ted
authors.initials: T
authors.lastname: Chang
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Kensington NSW 2052, Australia.
authors.forename: Hongxu
authors.initials: H
authors.lastname: Lu
authors.affiliation: Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales , Kensington NSW 2052, Australia.
authors.forename: Martina H
authors.identifier: 0000-0002-6433-4419
authors.initials: MH
authors.lastname: Stenzel
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001940:Breast / Q000187:drug effects / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D016466:CHO Cells; D002417:Cattle; D045744:Cell Line, Tumor; D003412:Cricetulus; D003474:Curcumin / Q000008:administration & dosage* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D011091:Polyesters; C016240:polycaprolactone; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D003474:Curcumin