title: Repeated biotransformation of glycerol to 1,3-dihydroxyacetone by immobilized cells of Gluconobacter oxydans with glycerol- and urea-feeding strategy in a bubble column bioreactor.
abstract: Some inorganic nitrogen sources and amino acids instead of yeast extract, which resulted in trouble of product purification, were introduced for 1,3-dihydroxyacetone (DHA) production by biotransformation with Gluconobacter oxydans. The results showed that urea is an optimal nitrogen source. Furthermore, the effects of glycerol- and urea-feeding strategies for DHA production by immobilized cells in a home-made bubble column bioreactor were optimized. Cells immobilization was prepared by cultivation in the bioreactor packed with porous ceramics, and then the broth was removed. Then, repeated biotransformation by continuous-feeding of glycerol and urea was developed. Up to 96.4±4.1g/L of average DHA concentration with 94.8±2.2% of average conversion rate of glycerol to DHA was achieved after 12 cycles of run. Near colorless DHA solution with few impurities was obtained and the production cost could be decreased.
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
authors.forename: Zhong-Ce
authors.initials: ZC
authors.lastname: Hu
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
authors.forename: Sheng-Ying
authors.initials: SY
authors.lastname: Tian
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Ruan
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China. Electronic address: zhengyg@zjut.edu.cn.
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D019149:Bioreactors; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism; D004098:Dihydroxyacetone / Q000096:biosynthesis*; D020600:Gluconobacter oxydans / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D014508:Urea / Q000378:metabolism
chemical_list: D014508:Urea; D004098:Dihydroxyacetone; D005990:Glycerol
keywords: 1,3-Dihydroxyacetone; Bubble column bioreactor; Gluconobacter oxydans; Glycerol- and urea-feeding strategy; Immobilization