title: Colectomy induces an aldosterone-mediated increase in jejunal glucose uptake in rats.
abstract: OBJECTIVE
  The main function of the colon is water and electrolyte absorption. Total colectomy eliminates this colonic function and may alter the absorptive capacity of the small intestine for nutrients. This study examines the effect of total colectomy on jejunal glucose absorption and investigates the potential role of aldosterone in mediating the alterations in glucose uptake post-colectomy using the aldosterone antagonist spironolactone.
  
  
  METHODS
  Total colectomy with ileo-rectal anastomosis was performed on anesthetized rats. Sham rats were identically handled without colon resection. Two days post-surgery, groups of colectomized rats were injected with either a daily subcutaneous dose of spironolactone or sesame oil for 12days. Body weight changes and food and water intake were measured in all experimental groups. Glucose absorption was measured by in-vivo single pass perfusion in the rat jejunum of control, sham, colectomized, colectomized with spironolactone, and colectomized with sesame oil treatment. Na/K ATPase, SGK1, SGLT1 and GLUT2 expressions were determined in jejunal mucosa in control, colectomized and colectomized/spironolactone injected rats by Western blot analysis. Histological assessment was performed on jejunal sections in control and colectomized groups.
  
  
  RESULTS
  Glucose absorption significantly increased in colectomized rats with an observed increase in Na/K ATPase and SGK1 expression. No significant expression change in SGLT1 and GLUT2 was detected in the jejunum in colectomized rats. Spironolactone, however, significantly decreased the glucose uptake post-colectomy and normalized Na/K ATPase and SGK1 expression.
  
  
  CONCLUSIONS
  Our results suggest that jejunal glucose uptake increases post-colectomy as a possible consequence of an aldosterone-mediated function.
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, El-Kurah, Lebanon. Electronic address: maha.khachab@balamand.edu.lb.
authors.forename: Maha
authors.initials: M
authors.lastname: Khachab
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, El-Kurah, Lebanon.
authors.forename: Amjad
authors.initials: A
authors.lastname: Kanaan
authors.affiliation: Faculty of Health Sciences, Lebanese University, Tripoli, Lebanon.
authors.forename: Dania
authors.initials: D
authors.lastname: Awad
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, El-Kurah, Lebanon.
authors.forename: Elie
authors.initials: E
authors.lastname: Deeba
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, El-Kurah, Lebanon.
authors.forename: Samira
authors.initials: S
authors.lastname: Osman
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, El-Kurah, Lebanon.
authors.forename: Camille F
authors.initials: CF
authors.lastname: Nassar
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003082:Colectomy / Q000009:adverse effects*; D003106:Colon / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004232:Diuretics / Q000633:toxicity; D005947:Glucose / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011183:Postoperative Complications; D051381:Rats; D017207:Rats, Sprague-Dawley; D012715:Sesame Oil / Q000494:pharmacology*; D013148:Spironolactone / Q000633:toxicity*
chemical_list: D004232:Diuretics; D013148:Spironolactone; D012715:Sesame Oil; D005947:Glucose
keywords: Aldosterone; Colectomy; Glucose absorption; In-vivo single pass perfusion; Jejunum