title: Oxidative stress and motility impairment in the semen of fertile males.
abstract: The aim of the study was to determine the total oxidant status (TOS) and evaluate the influence of oxidative stress on sperm quality in fertile males. The study population consisted of 55 fertile males. Based on the seminal plasma TOS value, the study subjects were divided into the two subgroups: a group with a low (TOS-L) and a high (TOS-H) value. Comparing the TOS-H group with the TOS-L group, we found poorer sperm motility in the TOS-H group. We found lower total antioxidant capacity values and lower activity levels in the majority of the determined superoxide dismutase, glutathione peroxidase, glutathione-S-transferase and glucose-6-phosphate dehydrogenase. Further, we found higher levels of copper and iron as well as lower levels of zinc in the TOS-H group. We observed lower medians of IL-2, 4, 6, 8 and INF-γ in the TOS-H group compared with the TOS-L group, whereas the medians of IL-1β, IL-10 and IL-12 were significantly higher. In fertile males, higher oxidative stress intensity was associated with poorer semen quality and decreased antioxidant capacity in semen. These negative effects might be a result of decreased activities of antioxidant enzymes and altered levels of trace metals and cytokines.
authors.affiliation: Department of Biochemistry, School of Medicine, Medical University of Silesia, Zabrze, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Dobrakowski
authors.affiliation: Department of Biochemistry, School of Medicine, Medical University of Silesia, Zabrze, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Kasperczyk
authors.affiliation: I-st Chair and Clinic of Gynecology, Obstetrics and Gynecological Oncology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Bytom, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Horak
authors.affiliation: Department of Biochemistry, School of Medicine, Medical University of Silesia, Zabrze, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Chyra-Jach
authors.affiliation: Department of Biochemistry, School of Medicine, Medical University of Silesia, Zabrze, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Birkner
authors.affiliation: Department of Biochemistry, School of Medicine, Medical University of Silesia, Zabrze, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Kasperczyk
mesh_terms: D000328:Adult; D002374:Catalase / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005298:Fertility / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D012661:Semen / Q000378:metabolism*; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016207:Cytokines; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: cytokine; fertility; oxidative stress; semen; total oxidant status