title: Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite.
abstract: Using a Au nanoparticle/reduced graphene oxide composite (AuNP-RGO), a signal-enhanced electrochemical immunosensor without label was created to detect neuron-specific enolase (NSE). Furthermore, an environmentally-friendly method was developed to prepare AuNP-RGO by employing chitosan (CS), which served as reducing and stabilizing agent. We showed that the sensitivity of the immunosensor designed in this report was remarkably enhanced because of the numerous active sites in the sensor provided by the AuNP-RGO nanostructure. For the quantification of NSE, the immunosensor exhibited a positive linear relationship with the concentration in the range of 0.1 to 2000 ng/mL, where the limit of the detection was 0.05 ng/mL.
authors.affiliation: Department of Oncology, Henan Academy institute of Traditional Chinese Medicine, Zhengzhou 450004, Henan, China. questwz@163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Oncology, Henan Academy institute of Traditional Chinese Medicine, Zhengzhou 450004, Henan, China. zhangjunping888@163.com.
authors.forename: Junping
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Henan Academy institute of Traditional Chinese Medicine, Zhengzhou 450004, Henan, China. zhangaihuatcm@163.com.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, Henan Province People's Hospital, Zhengzhou 450004, Henan, China. wang88982@126.com.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology, Henan Academy institute of Traditional Chinese Medicine, Zhengzhou 450004, Henan, China. caixiaoping987@126.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Cai
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology*; D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry*; D010751:Phosphopyruvate Hydratase / Q000276:immunology; D013057:Spectrum Analysis
chemical_list: D000951:Antigens, Neoplasm; D006046:Gold; D006108:Graphite; D048271:Chitosan; D010751:Phosphopyruvate Hydratase
keywords: electrochemical immunosensors; graphene; label-free immunosensor; neuron-specific enolase (NSE); signal-enhanced