title: In vivo analgesic, antipyretic and anti-inflammatory potential of leaf extracts and fractions of Eria javanica.
abstract: Background The objective of the study was to evaluate the antinociceptive, antipyretic and anti-inflammatory activities of ethanolic extract, methanolic extract and n-hexane and chloroform-soluble fractions of methanolic extract of Eria javanica leaves in animal model (rat and mice). Methods The anti-nociceptive potentials of the extracts were studied using the acetic acid-induced writhing test in mice and the antipyretic activity was investigated using yeast-induced pyrexia in rats. Anti-inflammatory activity test was done on rats at a dose by using carrageenan-induced paw edema test. Results In acetic acid-induced writhing inhibition study in Swiss albino mice, the crude methanolic extract at 200 mg/kg and 400 mg/kg doses and the n-hexane soluble fraction of crude methanolic extract at 400 mg/kg showed statistically significant activity with 53.21 % (p<0.001), 50.36 % (p<0.001) and 67.86 % (p<0.001) inhibition respectively compared to control. The crude ethanolic extract showed statistically significant antipyretic activity from 1 hours and onwards after administration at doses of 200 mg/kg body weight (p<0.005 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively) and 400 mg/kg body weight (p<0.05 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively). The crude methanolic extract showed statistically significant antipyretic activity from 2 hours and onwards at 400 mg/kg body weight (p<0.05 at 2nd hour and p<0.001 at 3rd and 4th hour respectively) and 200 mg/kg body weight dose showed statistically significant antipyretic activity from 3 hours and onward(p<0.001) in Brewer's yeast-induced pyrexia test in albino Wister rats. In carrageenan-induced rat's paw edema test, crude methanolic extract showed statistically significant anti-inflammatory activity from 2nd hour and onwards. The chloroform-soluble fraction of methanolic extract also showed significant activity from 1st hour onwards. Conclusions This study thereby indicates that leaves of E. javanica possess peripheral analgesic, antipyretic and anti-inflammatory activities and therefore a suitable candidate for further study.
authors.forename: N M Mahmudul Alam
authors.initials: NM
authors.lastname: Bhuiya
authors.forename: Mahmud
authors.initials: M
authors.lastname: Hasan
authors.forename: Zobaer Al
authors.initials: ZA
authors.lastname: Mahmud
authors.forename: Nazmul
authors.initials: N
authors.lastname: Qais
authors.forename: Mohammad Shah Hafez
authors.initials: MS
authors.lastname: Kabir
authors.forename: Farhan
authors.initials: F
authors.lastname: Ahmed
authors.forename: Mir Muhammad Nasir
authors.initials: MM
authors.lastname: Uddin
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D058633:Antipyretics / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D004487:Edema / Q000188:drug therapy; D005260:Female; D005334:Fever / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D051379:Mice; D059226:Nociceptive Pain / Q000188:drug therapy; D029595:Orchidaceae; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D017208:Rats, Wistar; D015003:Yeasts
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D058633:Antipyretics; D010936:Plant Extracts; D002351:Carrageenan; D019342:Acetic Acid