title: Both Hsp70 chaperone and Clp protease plastidial systems are required for protection against oxidative stress.
abstract: Environmental stress conditions such as high light, extreme temperatures, salinity or drought trigger oxidative stress and eventually protein misfolding in plants. In chloroplasts, chaperone systems refold proteins after stress, while proteases degrade misfolded and aggregated proteins that cannot be refolded. We observed that reduced activity of chloroplast Hsp70 chaperone or Clp protease systems both prevented growth of Arabidopsis thaliana seedlings after treatment with the oxidative agent methyl viologen. Besides showing a role for these particular protein quality control components on the protection against oxidative stress, we provide evidence supporting the existence of a yet undiscovered pathway for Clp-mediated degradation of the damaged proteins.
authors.affiliation: a Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB Bellaterra , Barcelona , Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pulido
authors.affiliation: a Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB Bellaterra , Barcelona , Spain.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Llamas
authors.affiliation: a Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB Bellaterra , Barcelona , Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodriguez-Concepcion
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D010269:Paraquat / Q000494:pharmacology; D018087:Plastids / Q000187:drug effects / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018840:HSP70 Heat-Shock Proteins; D010269:Paraquat
keywords: Chaperone; Clp; Hsp70; chloroplast; methyl viologen; oxidative stress; paraquat; protease; protein quality control