title: Modulation of chemotherapy-induced cytotoxicity in SH-SY5Y neuroblastoma cells by caffeine and chlorogenic acid.
abstract: Chemotherapy is an important treatment modality for malignancy but is limited by significant toxicity and it susceptibility to numerous drug interactions. While the interacting effects with medications are well known, there is limited evidence on the interaction with commonly consumed food and natural products. The aim of this study was to evaluate the bioactive constituents of coffee (caffeine and chlorogenic acid) on the cytotoxicity of doxorubicin, gemcitabine, and paclitaxel in vitro. Pretreatment with caffeine (100 nM and 10 μM) sensitized SH-SY5Y cells to doxorubicin-induced toxicity and increased apoptosis and sensitized PC3 cells to gemcitabine-induced toxicity. Pretreatment with 10 μM caffeine decreased total cell reactive oxygen species (ROS) production but increased mitochondrial ROS production. In contrast, caffeine (10 nM and 10 μM) protected cells against gemcitabine-induced toxicity and apoptosis. Similarly, 1 μM and 10 μM caffeine protected cells against paclitaxel-induced toxicity and mitochondrial ROS production. Chlorogenic acid had no effect on chemotherapy-induced toxicity in SH-SY5Y cells. In conclusion, this study provides preliminary evidence that caffeine, not chlorogenic acid, modulates the cytotoxicity of doxorubicin, gemcitabine, and paclitaxel in SH-SY5Y cells via different mechanisms.
authors.affiliation: a Menzies Health Institute Queensland, Griffith University , Gold Coast , QLD , Australia.|b School of Pharmacy , Griffith University , Gold Coast , QLD , Australia.|c Quality Use of Medicines Network , Gold Coast , QLD , Australia.
authors.forename: Susan
authors.initials: S
authors.lastname: Hall
authors.affiliation: a Menzies Health Institute Queensland, Griffith University , Gold Coast , QLD , Australia.|b School of Pharmacy , Griffith University , Gold Coast , QLD , Australia.|c Quality Use of Medicines Network , Gold Coast , QLD , Australia.
authors.forename: Shailendra
authors.initials: S
authors.lastname: Anoopkumar-Dukie
authors.affiliation: a Menzies Health Institute Queensland, Griffith University , Gold Coast , QLD , Australia.|b School of Pharmacy , Griffith University , Gold Coast , QLD , Australia.|c Quality Use of Medicines Network , Gold Coast , QLD , Australia.
authors.forename: Gary D
authors.initials: GD
authors.lastname: Grant
authors.affiliation: a Menzies Health Institute Queensland, Griffith University , Gold Coast , QLD , Australia.|d School of Allied Health Sciences , Griffith University , Gold Coast , QLD , Australia.
authors.forename: Ben
authors.initials: B
authors.lastname: Desbrow
authors.affiliation: a Menzies Health Institute Queensland, Griffith University , Gold Coast , QLD , Australia.|b School of Pharmacy , Griffith University , Gold Coast , QLD , Australia.
authors.forename: Richard
authors.initials: R
authors.lastname: Lai
authors.affiliation: a Menzies Health Institute Queensland, Griffith University , Gold Coast , QLD , Australia.|b School of Pharmacy , Griffith University , Gold Coast , QLD , Australia.|c Quality Use of Medicines Network , Gold Coast , QLD , Australia.
authors.forename: Devinder
authors.initials: D
authors.lastname: Arora
authors.affiliation: e Guangdong Pharmaceutical University , Guangzhou City , Guangdong , People's Republic of China.
authors.forename: Yinna
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002110:Caffeine / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002726:Chlorogenic Acid / Q000494:pharmacology*; D003841:Deoxycytidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology*; D004347:Drug Interactions; D006801:Humans; D017239:Paclitaxel / Q000008:administration & dosage / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D000093542:Gemcitabine
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D003841:Deoxycytidine; D002726:Chlorogenic Acid; D002110:Caffeine; D004317:Doxorubicin; D017239:Paclitaxel; D000093542:Gemcitabine
keywords: Caffeine; chemotherapy; chlorogenic acid; doxorubicin; gemcitabine; paclitaxel