title: The exposure of honey bees (Apis mellifera; Hymenoptera: Apidae) to pesticides: Room for improvement in research.
abstract: Losses of honey bees have been repeatedly reported from many places worldwide. The widespread use of synthetic pesticides has led to concerns regarding their environmental fate and their effects on pollinators. Based on a standardised review, we report the use of a wide variety of honey bee matrices and sampling methods in the scientific papers studying pesticide exposure. Matrices such as beeswax and beebread were very little analysed despite their capacities for long-term pesticide storage. Moreover, bioavailability and transfer between in-hive matrices were poorly understood and explored. Many pesticides were studied but interactions between molecules or with other stressors were lacking. Sampling methods, targeted matrices and units of measure should have been, to some extent, standardised between publications to ease comparison and cross checking. Data on honey bee exposure to pesticides would have also benefit from the use of commercial formulations in experiments instead of active ingredients, with a special assessment of co-formulants (quantitative exposure and effects). Finally, the air matrix within the colony must be explored in order to complete current knowledge on honey bee pesticide exposure.
authors.affiliation: Unit of Coordination and Support to Surveillance, ANSES, Scientific Affairs Department for Laboratories, Maisons-Alfort, France.
authors.forename: Johanna
authors.initials: J
authors.lastname: Benuszak
authors.affiliation: Unit of Honeybee Pathology, ANSES, European Union and National Reference Laboratory for Honeybee Health, Sophia Antipolis, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Laurent
authors.affiliation: Unit of Coordination and Support to Surveillance, ANSES, Scientific Affairs Department for Laboratories, Maisons-Alfort, France; Unit of Honeybee Pathology, ANSES, European Union and National Reference Laboratory for Honeybee Health, Sophia Antipolis, France. Electronic address: marie-pierre.chauzat@anses.fr.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Chauzat
mesh_terms: D000818:Animals; D001516:Bees / Q000502:physiology*; D004463:Ecology; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D054817:Pollination; D014885:Waxes
chemical_list: D004785:Environmental Pollutants; D010575:Pesticides; D014885:Waxes; C038228:beeswax
keywords: Acaricide; Active substance; Air; Metabolites; Plant protection products; Standardisation