title: Identification of a conserved conformational epitope in the VP2 protein of foot-and-mouth disease virus.
abstract: Foot-and-mouth disease (FMD), caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. VP2 is a structural protein of FMDV. In this study, a potent FMDV serotype-independent monoclonal antibody (MAb) 3D9 was generated. Screening of a phage-displayed random 12-peptide library revealed that MAb 3D9 bound to phages displaying a consensus motif GVYxxAYxW that is highly homologous to the89GVYxxxxxxxAYxxxxW105motif in the FMDV VP2 protein. Importantly, this conformational epitope was highly conserved among all seven serotypes of FMDV analyzed in sequence alignments. To further verify the authentic epitope recognized by MAb 3D9, a FMDV O/YS/CHA/05 mutant virus V90A was generated using a reverse genetics system. The results revealed that Val90was an important residue for MAb 3D9 binding within this conformational epitope. Thus, we finely mapped a conserved conformational epitope onto the FMDV VP2 protein. These results could be applied in the development of epitope-based vaccines and suitable MAb-based diagnostic methods for various FMDV serotype-independent tests.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China.
authors.forename: Baolin
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China.
authors.forename: Decheng
authors.initials: D
authors.lastname: Yang
authors.affiliation: Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, 151 Eastern Kelamayi Street, Urumqi, 830000, People's Republic of China.
authors.forename: Wenge
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China. ghzhoubt@163.com.
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Livestock Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, NO. 678 Haping Road Xiangfang District, Harbin, 150069, People's Republic of China. liyu@hvri.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D017124:Conserved Sequence; D006224:Cricetinae; D000939:Epitopes; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D000939:Epitopes; C442459:VP2 protein, Foot-and-mouth disease virus