title: Crystal Diagnostics Xpress S Kit for the Rapid Detection ofSalmonellaspp. in Selected Food Matrixes.
abstract: The Crystal Diagnostics (CDx) Xpress S Kit is a rapid-screening assay for Salmonella spp. in whole raw tomatoes, whole chicken carcasses, raw ground beef, raw beef trim, and whole liquid pasteurized eggs with citric acid when present at levels of 1 CFU/portion size. The Xpress S system comprises an automatic CDx Xpress Reader and a single-use CDx BioCassette that incorporates antibody-coupled microspheres and liquid crystal for the selective identification of the intended microbe. In internal evaluations, the CDx Xpress S Kit detected all 142 Salmonella strains tested, including non-enterica subspecies, and excluded all non-Salmonella species assayed. Method-developer studies, as well as a third-party evaluation, demonstrated that 15 h single-stage enrichment permits rapid detection equivalent to the U.S. Department of Agriculture and U.S. Food and Drug Administration reference methods. The results demonstrate that the CDx Xpress S Kit is one of the fastest, most sensitive, and most accurate methods for detecting Salmonella in food matrixes.
authors.affiliation: Crystal Diagnostics, Ltd, 510 Compton St, No. 106, Broomfield, CO 80020.
authors.forename: Brian
authors.initials: B
authors.lastname: Bullard
authors.affiliation: Crystal Diagnostics, Ltd, 510 Compton St, No. 106, Broomfield, CO 80020.
authors.forename: Curtis H
authors.initials: CH
authors.lastname: Stumpf
authors.affiliation: Crystal Diagnostics, Ltd, 510 Compton St, No. 106, Broomfield, CO 80020.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Crystal Diagnostics, Ltd, 510 Compton St, No. 106, Broomfield, CO 80020.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Kuzenko
authors.affiliation: Crystal Diagnostics, Ltd, 510 Compton St, No. 106, Broomfield, CO 80020.
authors.forename: Gary D
authors.initials: GD
authors.lastname: Niehaus
mesh_terms: D000818:Animals; D004531:Eggs / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D000069466:Red Meat / Q000032:analysis; D012475:Salmonella / Q000302:isolation & purification*