title: Rapid preparation of a noncultured skin cell suspension that promotes wound healing.
abstract: Autologous skin cell suspensions have been used for wound healing in patients with burns and against normal pigmentation in vitiligo. To separate cells and the extracellular matrix from skin tissue, most researchers use enzymatic digestion. Therefore, this process is difficult to perform during a routine surgical procedure. We aimed to prepare a suspension of noncultured autologous skin cells (NCSCs) using a tissue homogenizer as a new method instead of harsh biochemical reagents. The potential clinical applicability of NCSCs was analyzed using a nude-rat model of burn healing. After optimization of the homogenizer settings, cell viability ranged from 52 to 89%. Scanning electron microscopy showed evidence of keratinocyte-like cell morphology, and several growth factors, including epidermal growth factor and basic fibroblast growth factor, were present in the NCSCs. The rat model revealed that NCSCs accelerated skin regeneration. NCSCs could be generated using a tissue homogenizer for enhancement of wound healing in vivo. In the NCSC group of wounds, on day 7 of epithelialization, granulation was observed, whereas on day 14, there was a significant increase in skin adnexa regeneration as compared to the control group (PBS treatment; p < 0.05). This study suggests that the proposed process is rapid and does not require the use of biochemical agents. Thus, we recommend a combination of surgical treatment with the new therapy for a burn as an effective method.
authors.affiliation: Department of Emergency Medicine, Yonsei University College of Medicine, Seoul, Korea.|Bestian Seoul Hospital, Seoul, Korea.
authors.forename: Cheonjae
authors.initials: C
authors.lastname: Yoon
authors.affiliation: Bestian R&D Center, Medical Group Bestian, Daejeon, Korea. nostalgie@naver.com.
authors.forename: Jungsuk
authors.initials: J
authors.lastname: Lee
authors.affiliation: Bestian R&D Center, Medical Group Bestian, Daejeon, Korea.
authors.forename: Hyosun
authors.initials: H
authors.lastname: Jeong
authors.affiliation: Bestian R&D Center, Medical Group Bestian, Daejeon, Korea.
authors.forename: Sungjun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Bestian R&D Center, Medical Group Bestian, Daejeon, Korea.
authors.forename: Taesik
authors.initials: T
authors.lastname: Sohn
authors.affiliation: Department of Emergency Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Sungphil
authors.initials: S
authors.lastname: Chung
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis; D015603:Keratinocytes / Q000166:cytology*; D008297:Male; D051381:Rats; D011923:Rats, Nude; D012038:Regeneration; D012867:Skin / Q000166:cytology* / Q000473:pathology; D012879:Skin Physiological Phenomena; D016038:Skin Transplantation / Q000379:methods*; D014945:Wound Healing
chemical_list: D036341:Intercellular Signaling Peptides and Proteins
keywords: Non-cultured skin cells; On-site therapy; Skin cells therapy; Wound healing