title: MicroRNA-182 prevents vascular smooth muscle cell dedifferentiation via FGF9/PDGFRβ signaling.
abstract: The abnormal phenotypic transformation of vascular smooth muscle cells (SMCs) causes various proliferative vascular diseases. MicroRNAs (miRNAs or miRs) have been established to play important roles in SMC biology and phenotypic modulation. This study revealed that the expression of miR‑182 was markedly altered during rat vascular SMC phenotypic transformation in vitro. We aimed to investigate the role of miR‑182 in the vascular SMC phenotypic switch and to determine the potential molecular mechanisms involved. The expression of miR‑182 gene was significantly downregulated in cultured SMCs during dedifferentiation from a contractile to a synthetic phenotype. Conversely, the upregulation of miR‑182 increased the expression of SMC-specific contractile genes, such as α-smooth muscle actin, smooth muscle 22α and calponin. Additionally, miR‑182 overexpression potently inhibited SMC proliferation and migration under both basal conditions and under platelet-derived growth factor-BB stimulation. Furthermore, we identified fibroblast growth factor 9 (FGF9) as the target gene of miR‑182 for the phenotypic modulation of SMCs mediated through platelet-derived growth factor receptor β (PDGFRβ) signaling. These data suggest that miR‑182 may be a novel SMC phenotypic marker and a modulator that may be used to prevent SMC dedifferentiation via FGF9/PDGFRβ signaling.
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Nana
authors.initials: N
authors.lastname: Dong
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Jinwei
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Zulong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lv
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: Key Laboratory of Myocardial Ischemia, Ministry of Education, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Shaohong
authors.initials: S
authors.lastname: Fang
authors.affiliation: Key Laboratory of Myocardial Ischemia, Ministry of Education, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Jiangtian
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory of Myocardial Ischemia, Ministry of Education, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Hulun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Second Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D051525:Fibroblast Growth Factor 9 / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C496461:Fgf9 protein, rat; D051525:Fibroblast Growth Factor 9; C577631:MIRN182 microRNA, rat; D035683:MicroRNAs; D020797:Receptor, Platelet-Derived Growth Factor beta