title: IL-33 induces both regulatory B cells and regulatory T cells in dextran sulfate sodium-induced colitis.
abstract: Interleukin (IL)-33 is a member of the IL-1 family. Serum levels of IL-33 are increased in inflammatory bowel diseases (IBD), suggesting that IL-33 is involved in the pathogenesis of IBD, although its role is not clear. In this study, we investigated the role of IL-33 in the regulation of T-helper (Th) cell and B cell responses in mesenteric lymph nodes (MLN) in mice with dextran sulfate sodium (DSS)-induced colitis. Here, we showed that IL-33-treated mice were susceptible to DSS-induced colitis as compared with PBS-treated mice. The production of spontaneous inflammatory cytokines production by macrophages or dendritic cells (DC) in MLN significantly increased, and the responses of Th2, regulatory T cells (Treg) and regulatory B cells (Breg) were markedly upregulated, while Th1 responses were significantly downregulated in MLN of IL-33-treated mice with DSS-induced colitis. Our results demonstrate that IL-33 contributes to the pathogenesis of DSS-induced colitis in mice by promoting Th2 responses, but suppressing Th1 responses, in MLN. Moreover, IL-33 treatment increased Breg and Treg responses in MLN in mice with DSS-induced colitis. Therefore, modulation of IL-33/ST2 signaling is implicated as a novel biological therapy for inflammatory diseases associated with Th1 responses.
authors.affiliation: Life Science School, Liaoning University, Shenyang, China; Department of Immunology, China Medical University, Shenyang, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Northeast Pharmaceutical Group Co., Ltd., Shenyang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Xinrui
authors.initials: X
authors.lastname: Meng
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Siyao
authors.initials: S
authors.lastname: Chen
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Wang
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: cllu@mail.cmu.edu.cn.
authors.forename: Changlong
authors.initials: C
authors.lastname: Lu
authors.affiliation: Life Science School, Liaoning University, Shenyang, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D060151:B-Lymphocytes, Regulatory / Q000276:immunology*; D002478:Cells, Cultured; D003092:Colitis / Q000139:chemically induced / Q000276:immunology*; D003106:Colon / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology*; D000072179:Interleukin-1 Receptor-Like 1 Protein / Q000378:metabolism; D000067596:Interleukin-33 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015398:Signal Transduction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D058408:Th1-Th2 Balance; D018418:Th2 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; C486189:Il1rl1 protein, mouse; C506535:Il33 protein, mouse; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D016264:Dextran Sulfate
keywords: IL-33; Inflammatory bowel diseases; Regulatory B cell; Regulatory T cell