title: Mini-column assay for rapid detection of malachite green in fish.
abstract: A simple, rapid and economical mini-column method for detecting malachite green (MG) residue in fish was developed. The method used a column with 2mm ID that was tightly packed with silica gel followed by alumina. Detection of MG was performed by viewing the developed mini-column at visible light by naked eye; where MG was seen as compact green band at the confluence of the silica gel layer with alumina layer. The limit of detection of the assay was 2ng which conform the minimum required performance limit (MRPL). Evaluation utility of the method indicated that all blank and spiked samples at levels below MRPL were assessed as accepted. The intensity of the green band increased whenever MG level in the extract increased; indicated that suggested mini-column technique could be used for semi-quantitative determination of MG in fish samples. The method can be used to select the questionable samples.
authors.affiliation: Food Science and Technology Dept., National Research Centre, Dokki, Cairo, Egypt. Electronic address: dr.ali.shalaby@Gmail.com.
authors.forename: Ali R
authors.initials: AR
authors.lastname: Shalaby
authors.affiliation: Food Science and Technology Dept., National Research Centre, Dokki, Cairo, Egypt.
authors.forename: Wafaa H
authors.initials: WH
authors.lastname: Emam
authors.affiliation: Plant Research Dept., Nuclear Research Center, Atomic Energy Authority, P. No. 13759, Cairo, Egypt.
authors.forename: Mervat M
authors.initials: MM
authors.lastname: Anwar
mesh_terms: D000537:Aluminum Oxide; D002853:Chromatography, Liquid / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D057230:Limit of Detection; D012394:Rosaniline Dyes / Q000032:analysis / Q000302:isolation & purification*; D058428:Silica Gel
chemical_list: D012394:Rosaniline Dyes; C005095:malachite green; D058428:Silica Gel; D000537:Aluminum Oxide
keywords: Chromatography; Fish; Malachite green; Mini-column