title: Sequence requirements of oligonucleotide chiral selectors for the capillary electrophoresis resolution of low-affinity DNA binders.
abstract: We recently reported that a great variety of DNA oligonucleotides (ONs) used as chiral selectors in partial-filling capillary electrophoresis (CE) exhibited interesting enantioresolution properties toward low-affinity DNA binders. Herein, the sequence prerequisites of ONs for the CE enantioseparation process were studied. First, the chiral resolution properties of a series of homopolymeric sequences (Poly-dT) of different lengths (from 5 to 60-mer) were investigated. It was shown that the size increase-dependent random coil-like conformation of Poly-dT favorably acted on the apparent selectivity and resolution. The base-unpairing state constituted also an important factor in the chiral resolution ability of ONs as the switch from the single-stranded to double-stranded structure was responsible for a significant decrease in the analyte selectivity range. Finally, the chemical diversity enhanced the enantioresolution ability of single-stranded ONs. The present work could lay the foundation for the design of performant ON chiral selectors for the CE separation of weak DNA binder enantiomers.
authors.affiliation: DPM, UMR 5063, Université Grenoble Alpes, CNRS, Grenoble, France.
authors.forename: Luma
authors.initials: L
authors.lastname: Tohala
authors.affiliation: DPM, UMR 5063, Université Grenoble Alpes, CNRS, Grenoble, France.
authors.forename: Farid
authors.initials: F
authors.lastname: Oukacine
authors.affiliation: DPM, UMR 5063, Université Grenoble Alpes, CNRS, Grenoble, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Ravelet
authors.affiliation: DPM, UMR 5063, Université Grenoble Alpes, CNRS, Grenoble, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Peyrin
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D011071:Poly T / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism
chemical_list: D009841:Oligonucleotides; D011071:Poly T
keywords: Capillary electrophoresis; Chiral selectors; Oligonucleotides; Partial-filling