title: In situ cellular level Raman spectroscopy of the thyroid.
abstract: We report a novel Raman spectroscopy method for in situ cellular level analysis of the thyroid. Thyroids are harvested from control and lithium treated mice. Lithium is used to treat bipolar disorder, but affects thyroid function. Raman spectra are acquired with a confocal setup (514 nm laser, 20 µm spot) focused on a follicular lumen. Raman peaks are observed at 1440, 1656, and 1746 cm-1, corresponding to tyrosine, an important amino acid for protein synthesis. Peaks are also observed at 563, 1087, 1265 and 1301 cm-1. With lithium, the tyrosine peaks increase, indicating tyrosine buildup. Raman spectroscopy can study the impact of many exogenous treatments on thyroid biochemistry.
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Alan Wing Lun
authors.initials: AW
authors.lastname: Law
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Rafay
authors.initials: R
authors.lastname: Ahmed
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Tsz Wing
authors.initials: TW
authors.lastname: Cheung
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Chun Yu
authors.initials: CY
authors.lastname: Mak
authors.affiliation: Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region, China.
authors.forename: Condon
authors.initials: C
authors.lastname: Lau
keywords: (140.3550) Lasers, Raman; (180.5655) Raman microscopy; (300.6450) Spectroscopy, Raman