title: The antifungal activity and cytotoxicity of silver containing denture base material.
abstract: Denture base materials are susceptible to fungal adhesion, which is an important etiological issue in the pathogenesis of denture stomatitis. The purpose of this in vitro study was to evaluate the antifungal activity and cytotoxicity of denture base material containing silver microparticles.
  
  
  
  The polymethyl methacrylate (PMMA) denture base material was used, and silver microparticles were added to the polymer powder in different concentrations by volume (0%, 0.25%, 0.5%, and 1%). Their antifungal activity against Candida albicans was assessed in terms of colony-forming units. PMMA disc specimens containing silver microparticles were eluted with culture medium for 1, 2, and 5 days. The cytotoxicity of the eluates to cultured L929 mouse fibroblast cells was evaluated using a real-time cell analysis (RTCA) system and the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay.
  
  
  
  The antifungal effect against C. albicans increased with the percentage of silver microparticles (P < 0.05). For both tests, both RTCA and the MTT assay, no time- or silver-dependent cytotoxicity of PMMA denture base material containing silver microparticles was observed.
  
  
  
  PMMA denture base material containing silver microparticles have antifungal activity and no cytotoxic effect.
authors.affiliation: Department of Prosthodontics, Faculty of Dentistry, Bezmialem Vakif University, Istanbul, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Kurt
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Erkose-Genc
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Uzun
authors.affiliation: Department of Genetics, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Emrence
authors.affiliation: Department of Medical Genetics and REMER, School of Medicine, Medipol University, Istanbul, Turkey.
authors.forename: D
authors.initials: D
authors.lastname: Ustek
authors.affiliation: Department of Prosthodontics, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Isik-Ozkol
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D003819:Denture Bases; D006801:Humans; D008826:Microbial Sensitivity Tests; D019904:Polymethyl Methacrylate / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D012834:Silver; D019904:Polymethyl Methacrylate