title: Impact of heat shock step on bacterial transformation efficiency.
abstract: CaCl2treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain ofEscherichia coli(E. coli) was being susceptible using CaCl2treatment. Some Cells were kept at -80oC while the others were kept at 4 ˚C. Afterwards the susceptible cells were transformed using either standard or lab protocol. The transformation efficiency between cells experienced heat shock and those were not influenced by heat shock was almost the same. Moreover, regardless of transformation protocol, the cells kept at 4 ˚C were transformed more efficiently in compared to those were kept at -80oC.
authors.affiliation: Department of Nanobiotechnology, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Maral
authors.initials: M
authors.lastname: Rahimzadeh
authors.affiliation: Department of Molecular Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Sadeghizadeh
authors.affiliation: Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran.
authors.forename: Farhood
authors.initials: F
authors.lastname: Najafi
authors.affiliation: Department of Biophysics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyed
authors.initials: S
authors.lastname: Arab
authors.affiliation: 5) Laboratory of Membrane Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Mobasheri
keywords: Artificial transformation; E. coli; Heat shock; Transformation efficiency