title: Characterization of a New Trioxilin and a Sulfoquinovosyl Diacylglycerol with Anti-Inflammatory Properties from the Dinoflagellate Oxyrrhis marina.
abstract: Two new compounds-a trioxilin and a sulfoquinovosyl diacylglycerol (SQDG)-were isolated from the methanolic extract of the heterotrophic dinoflagellateOxyrrhis marinacultivated by feeding on dried yeasts. The trioxilin was identified as (4Z,8E,13Z,16Z,19Z) -7(S),10(S),11(S)-trihydroxydocosapentaenoic acid (1), and the SQDG was identified as (2S)-1-O-hexadecanosy-2-O-docosahexaenoyl-3-O-(6-sulfo-α-d-quinovopyranosyl)-glycerol (2) by a combination of nuclear magnetic resonance (NMR) spectra, mass analyses, and chemical reactions. The two compounds were associated with docosahexaenoic acid, which is a major component ofO. marina. The two isolated compounds showed significant nitric oxide inhibitory activity on lipopolysaccharide-induced RAW264.7 cells. Compound2showed no cytotoxicity against hepatocarcinoma (HepG2), neuroblastoma (Neuro-2a), and colon cancer (HCT-116) cells, while weak cytotoxicity was observed for compound1against Neuro-2a cells.
authors.affiliation: Environment and Resource Convergence Center, Advanced Institutes of Convergence Technology, Suwon 16229, Korea. journal04@snu.ac.kr.|School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, Korea. journal04@snu.ac.kr.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Yoon
authors.affiliation: Department of Oceanography, Kunsan National University, San 68 Miryong-dong, Kunsan, JeonBuk 573-701, Korea. yar0241@naver.com.
authors.forename: A Reum
authors.initials: AR
authors.lastname: Yang
authors.affiliation: Environment and Resource Convergence Center, Advanced Institutes of Convergence Technology, Suwon 16229, Korea. bada0@snu.ac.kr.
authors.forename: Jaeyeon
authors.initials: J
authors.lastname: Park
authors.affiliation: Environment and Resource Convergence Center, Advanced Institutes of Convergence Technology, Suwon 16229, Korea. sjmoon04@snu.ac.kr.
authors.forename: Seung Joo
authors.initials: SJ
authors.lastname: Moon
authors.affiliation: Department of Agronomy & Medicinal plant Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea. ejjeong@gntech.ac.kr.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Jeong
authors.affiliation: Department of Oceanography, Kunsan National University, San 68 Miryong-dong, Kunsan, JeonBuk 573-701, Korea. jrrho@kunsan.ac.kr.
authors.forename: Jung-Rae
authors.initials: JR
authors.lastname: Rho
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D002460:Cell Line; D003110:Colonic Neoplasms / Q000188:drug therapy; D004075:Diglycerides / Q000494:pharmacology*; D004141:Dinoflagellida / Q000737:chemistry*; D045325:HCT116 Cells; D056945:Hep G2 Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000188:drug therapy
chemical_list: D000893:Anti-Inflammatory Agents; D004075:Diglycerides; D008070:Lipopolysaccharides; C003367:sulphoquinovosyl-diacylglycerol
keywords: Oxyrrhis marina; docosahexaenoic acid; nitric oxide inhibition; sulfoquinovosyl diacylglycerols; trioxilin