title: DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection 'DNA-Directed Antibody Immobilization.
abstract: Protein microarrays are emerging tools which have become very powerful in multiplexed detection technologies. A variety of proteins can be immobilized on a sensor chip allowing for multiplexed diagnostics. Therefore, various types of analyte in a small volume of sample can be detected simultaneously. Protein immobilization is a crucial step for creating a robust and sensitive protein microarray-based detection system. In order to achieve a successful protein immobilization and preserve the activity of the proteins after immobilization, DNA-directed immobilization is a promising technique. Here, we present the design and the use of DNA-directed immobilized (DDI) antibodies in fabrication of robust protein microarrays. We focus on application of protein microarrays for capturing and detecting nanoparticles such as intact viruses. Experimental results on Single-particle interferometric reflectance imaging sensor (SP-IRIS) are used to validate the advantages of the DDI method.
authors.affiliation: Biomedical Engineering Department, Boston University, Boston, MA, 02215, USA.|Faculty of Medicine, Bahcesehir University, Istanbul, 34353, Turkey.
authors.forename: Nese Lortlar
authors.initials: NL
authors.lastname: Ünlü
authors.affiliation: Electrical and Computer Engineering Department, Boston University, Boston, MA, 02215, USA.
authors.forename: Fulya Ekiz
authors.initials: FE
authors.lastname: Kanik
authors.affiliation: Biotechnology Research Program Department, ASELSAN Research Center, Ankara, 06370, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Seymour
authors.affiliation: Microbiology Department, Boston University School of Medicine, 8 St Mary's St, Boston, MA, 02215, USA.
authors.forename: John H
authors.initials: JH
authors.lastname: Connor
authors.affiliation: Biomedical Engineering Department, Boston University, Boston, MA, 02215, USA. selim@bu.edu.|Electrical and Computer Engineering Department, Boston University, Boston, MA, 02215, USA. selim@bu.edu.|Microbiology Department, Boston University School of Medicine, 8 St Mary's St, Boston, MA, 02215, USA. selim@bu.edu.
authors.forename: M Selim
authors.initials: MS
authors.lastname: Ünlü
mesh_terms: D055910:Antibodies, Immobilized; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA; D015342:DNA Probes; D007091:Image Processing, Computer-Assisted / Q000379:methods; D018796:Immunoconjugates; D044085:Microfluidics / Q000295:instrumentation; D008853:Microscopy / Q000379:methods; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D013223:Statistics as Topic / Q000379:methods; D014780:Viruses / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D015342:DNA Probes; D018796:Immunoconjugates; D004247:DNA
keywords: DNA-directed antibody immobilization; Label free detection; Protein microarrays; SP-IRIS; Single particle detection