title: Aberrant Immunological Synapses Driven by Leukemic Antigen-Presenting Cells.
abstract: Aberrant immune synapse formation between antigen-presenting and immune effector cells is a central mediator of immune dysfunction and can be observed across several haematologic malignancies. Here, we describe the cell preparation, conjugation and immune synapse quantification of B and T cells obtained from patients with leukaemia and the adaptions required when using cells from murine models of disease.
authors.affiliation: Department of Haemato-Oncology, Barts Cancer Institute, Queen Mary University of London, 3rd Floor John Vane Science Centre, Charterhouse Square, London, UK.|Comprehensive Cancer Center, The Ohio State University, EC1M 6BQ, Columbus, OH, USA.
authors.forename: Fabienne McClanahan
authors.initials: FM
authors.lastname: Lucas
authors.affiliation: Department of Haemato-Oncology, Barts Cancer Institute, Queen Mary University of London, 3rd Floor John Vane Science Centre, Charterhouse Square, London, UK. j.gribben@qmul.ac.uk.
authors.forename: John G
authors.initials: JG
authors.lastname: Gribben
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000473:pathology; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D054992:Immunological Synapses / Q000276:immunology* / Q000473:pathology; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
keywords: B cells; Eμ-TCL1 model; Immune synapse; Leukaemia; Lymphoma; NK cells; T cells