title: Segmentation of nucleus and cytoplasm of a single cell in three-dimensional tomogram using optical coherence tomography.
abstract: A random rayburst sampling (RRBS) framework was developed to detect the nucleus and cell membrane boundaries in three-dimensional (3-D) space. Raw images were acquired through a full-field optical coherence tomography system with submicron resolution—i.e., 0.8 ?? ? m in lateral and 0.9 ?? ? m in axial directions. The near-isometric resolution enables 3-D segmentation of a nucleus and cell membrane for determining the volumetric nuclear-to-cytoplasmic (N/C) ratio of a single cell. The RRBS framework was insensitive to the selection of seeds and image pixel noise. The robustness of the RRBS framework was verified through the convergence of the N/C ratio searching algorithm. The relative standard deviation of the N/C ratio between different randomly selected seed sets was only 2%. This technique is useful for various in vitro assays on single-cell analyses.
authors.affiliation: National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, Taiwan.
authors.forename: Chia-Kai
authors.initials: CK
authors.lastname: Chang
authors.affiliation: National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, Taiwan.
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Tsai
authors.affiliation: National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, Taiwan.
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Hsu
authors.affiliation: National Taiwan University, Department of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
authors.forename: Jau-Shiuh
authors.initials: JS
authors.lastname: Chen
authors.affiliation: National Taiwan University, Department of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Liao
authors.affiliation: National Taiwan University, Department of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
authors.forename: Yi-Shuan
authors.initials: YS
authors.lastname: Sheen
authors.affiliation: National Taiwan University, Department of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
authors.forename: Jin-Bon
authors.initials: JB
authors.lastname: Hong
authors.affiliation: National Taiwan University, Department of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
authors.forename: Ming-Yi
authors.initials: MY
authors.lastname: Lin
authors.affiliation: National Taiwan University, Department of Dermatology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
authors.forename: Jeng-Wei
authors.initials: JW
authors.lastname: Tjiu
authors.affiliation: National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, Taiwan.
authors.forename: Sheng-Lung
authors.initials: SL
authors.lastname: Huang
mesh_terms: D000465:Algorithms; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D041623:Tomography, Optical Coherence