title: Isolation and identification of two new compounds from marine-derived fungus Acremonium fusidioides RZ01.
abstract: Two new compounds, (22E)-25-carboxy-8β,14β-epoxy-4α,5α-dihydroxyergosta-2,22-dien-7-one (1) and fusidione (3), along with two known compounds, 5α,8α-epidioxy ergosta-6,22-diene-3β-ol (2) and microperfuranone (4), were isolated from the fermentation products of the marine-sourced fungus Acremonium fusidioides RZ01. The structures of compounds 1 and 3 were elucidated by extensive spectroscopic methods, especially 2D NMR, and their absolute configurations were suggested on the basis of the circular dichroism spectral analysis and the NOESY data. Both new compounds showed inhibitory activity against HL-60 cells with IC50values being16.6 and 44.9 μmol·L-1, respectively.
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: An
authors.affiliation: School of Life Sciences and Biotechnology, Dalian University, Dalian 116622, China.
authors.forename: Bao-Min
authors.initials: BM
authors.lastname: Feng
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Shao-Fei
authors.initials: SF
authors.lastname: Chen
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: wanghaifeng0310@163.com.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: peiyueh@vip.163.com.
authors.forename: Yue-Hu
authors.initials: YH
authors.lastname: Pei
mesh_terms: D000164:Acremonium / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D005285:Fermentation; D018922:HL-60 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012623:Seawater / Q000382:microbiology*
chemical_list: D000970:Antineoplastic Agents
keywords: Acremonium fusidioides; Chemical constituent; Fusidione; Steroid