title: Repeated Cold Stress Reduces Cyclophosphamide-Induced Cystitis/Bladder Pain and Macrophage Activity in Mice.
abstract: We examined the effect of repeated cold (RC) stress on cyclophosphamide (CPA)-induced cystitis/bladder pain in mice, in relation to macrophage activity. CPA, given i.p. at 400 mg/kg, caused bladder pain symptoms accompanying cystitis in both unstressed and RC-stressed mice, which were prevented by the macrophage inhibitor minocycline. A low dose, that is, 200 mg/kg, of CPA still produced bladder pain symptoms in unstressed but not RC-stressed mice. Lipopolysaccharide-induced cytokine production in peritoneal macrophages from RC-stressed mice was less than that from unstressed mice. Thus, RC stress appears to reduce CPA-induced bladder pain in mice, which may be associated with the decreased macrophage activity.
authors.affiliation: Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University (Formerly Kinki University), Higashi-osaka, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Tsubota
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Saki
authors.initials: S
authors.lastname: Hiruma
authors.forename: Haruka
authors.initials: H
authors.lastname: Saeki
authors.forename: Takaya
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Fumiko
authors.initials: F
authors.lastname: Sekiguchi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Funakami
authors.forename: Atsufumi
authors.initials: A
authors.lastname: Kawabata
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003520:Cyclophosphamide / Q000009:adverse effects*; D003556:Cystitis / Q000139:chemically induced* / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008911:Minocycline / Q000494:pharmacology; D010146:Pain / Q000139:chemically induced* / Q000276:immunology; D013312:Stress, Physiological; D001743:Urinary Bladder / Q000187:drug effects* / Q000276:immunology
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D003520:Cyclophosphamide; D008911:Minocycline