title: In vivoimaging of hepatic hemodynamics and light scattering property during ischemia-reperfusion in rats based on spectrocolorimetry.
abstract: A red-green-blue camera-based imaging method is proposed for estimating spatial maps of concentrations of oxyhemoglobin (CHbO), deoxyhemoglobin (CHbR), total hemoglobin (CHbT), tissue oxygen saturation (StO2), and scattering power (b) in liver tissue. Hemodynamic responses to hepatic ischemia-reperfusion ofin vivorat liver tissues induced by portal triad occlusion were evaluated. Upon portal triad occlusion, this method yielded images of decreasedCHbO,CHbT,StO2, andb, and increasedCHbRfollowed by a progressive increase inCHbOandStO2during reperfusion. Time courses of the changes inCHbO,CHbR,CHbT, andStO2over different regions of interest (ROIs) revealed that ischemia results in an abrupt significant (P<0.05) reduction inCHbO,CHbT, andStO2with a simultaneous increase inCHbRcompared to the baseline level, indicative of the hemodynamic responses during hepatic ischemia-reperfusion. Upon reperfusion, there was a gradual increase inCHbOandStO2, and decrease inCHbR. The change in average scattering powerbimplies the presence of morphological alterations in the cellular and subcellular structures induced by ischemia or anoxia. This study shows the potential of monitoring spatiotemporal changes in hemodynamic parameters and morphological changes in studies of hepatic pathophysiology.
authors.affiliation: Graduate School of Bio-Applications & Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Sharmin
authors.initials: S
authors.lastname: Akter
authors.affiliation: Division of Biomedical Information Sciences, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Kawauchi
authors.affiliation: Division of Biomedical Information Sciences, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Sato
authors.affiliation: Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Suefumi
authors.initials: S
authors.lastname: Aosasa
authors.affiliation: Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Yamamoto
authors.affiliation: Graduate School of Bio-Applications & Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Izumi
authors.initials: I
authors.lastname: Nishidate
keywords: (170.0110) Imaging systems; (170.0170) Medical optics and biotechnology; (170.3010) Image reconstruction techniques; (170.3880) Medical and biological imaging; (170.6510) Spectroscopy, tissue diagnostics