title: Analyses of natural variation indicates that the absence of RPS4/RRS1 and amino acid change in RPS4 cause loss of their functions and resistance to pathogens.
abstract: A pair of Arabidopsis thaliana resistance proteins, RPS4 and RRS1, recognizes the cognate Avr effector from the bacterial pathogens Pseudomonas syringae pv. tomato expressing avrRps4 (Pst-avrRps4), Ralstonia solanacearum, and the fungal pathogen Colletotrichum higginsianum and leads to defense signaling activation against the pathogens. In the present study, we analyzed 14 A. thaliana accessions for natural variation in Pst-avrRps4 and C. higginsianum susceptibility, and found new compatible and incompatible Arabidopsis-pathogen interactions. We first found that A. thaliana accession Cvi-0 is susceptible to Pst-avrRps4. Interestingly, the genome sequence assembly indicated that Cvi-0 lost both RPS4 and RRS1, but not RPS4B and RRS1B, compared to the reference genome sequence from A. thaliana accession Col-0. On the other hand, the natural variation analysis of RPS4 alleles from various Arabidopsis accessions revealed that one amino-acid change, Y950H, is responsible for the loss of resistance to Pst-avrRps4 and C. higginsianum in RLD-0. Our data indicate that the amino acid change, Y950H, in RPS4 resulted in the loss of both RPS4 and RRS1 functions and resistance to pathogens.
authors.affiliation: a Research Institute for Biological Sciences Okayama , Okayama , Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.affiliation: b RIKEN BioResource Centre , Tsukuba , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iuchi
authors.affiliation: a Research Institute for Biological Sciences Okayama , Okayama , Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Narusaka
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020231:Colletotrichum / Q000472:pathogenicity; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D044224:Pseudomonas syringae / Q000472:pathogenicity; D043368:Ralstonia solanacearum / Q000472:pathogenicity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; C000604874:RPS4B protein, Arabidopsis; C000604873:TTR1 protein, Arabidopsis; D014157:Transcription Factors; C097421:rps4 protein, plant
keywords: Arabidopsis; R gene; RPS4; RRS1; colletotrichum higginsianum; pseudomonas syringae