title: Synergistic efficacy of Cullin1 and MMP-2 expressions in diagnosis and prognosis of colorectal cancer.
abstract: BACKGROUND
  Cullin1 and MMP-2 have been identified as important markers in various cancers, but their roles in colorectal cancer (CRC) have remained to be discovered. The aim of this study was to investigate the expression pattern and significance of Cullin1 and MMP-2 in CRC.
  
  
  METHODS
  A total of 470 CRC patients were enrolled. Archival paraffin-embedded CRC tissue samples were used to generate tissue microarray blocks, which were immunohistochemically stained for Cullin1 and MMP-2. Prognostic and predictive role of Cullin1 and MMP-2 expression was evaluated by univariate and multivariate analysis, respectively. Values of p < 0.05 were considered statistically significant.
  
  
  RESULTS
  Cullin1 and MMP-2 protein levels were significantly upregulated in CRC tissues compared with adjacent noncancerous tissues. High tumoral Cullin1 or MMP-2 expression significantly correlated with shorter overall survival (OS), as well as with clinicopathologic characteristics in patients. Multivariate regression analysis showed that high Cullin1 and MMP-2 expressions, separately and together, were independent negative markers of OS.
  
  
  CONCLUSIONS
  Cullin1 and MMP-2 expressions could be novel diagnostic and prognostic markers for CRC patients.
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.|Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.|Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Pathology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.|Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Qian
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Radiology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D044842:Cullin Proteins / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D016612:Paraffin Embedding; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C100318:Cullin 1; D044842:Cullin Proteins; D020778:Matrix Metalloproteinase 2
keywords: Cullin1; MMP-2; colorectal cancer; diagnosis; prognosis