title: Combined Haplotypes of CaSR Gene Sequence Variants and Their Associations with Growth Traits in Cattle.
abstract: The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in cattle breeds. Four novel single nucleotide polymorphisms (SNPs) and one previously reported SNP (NC_007299.5: g.67630865T>C, 67638409G>C, 67660395G>C, 67661546C>G, and 67661892A>C) were identified in the bovine CaSR gene using DNA sequencing and PCR-SSCP methods in 520 individuals from three representative breeds. The three SNP P4_2, P7_1, and P7_4 in LX, QC, and JX cattle populations belonged to intermediate genetic diversity (0.25 < PIC < 0.5). In addition, we evaluated the haplotype frequency and linkage disequilibrium coefficient of five sequence variants in the three cattle breeds. LD and haplotype structure of CaSR were different between breeds. LD analysis showed that the P4_2 and P7_4 loci were in complete LD in JX cattle population (r2 = 0.99 and D' = 1). Only 11 haplotypes were listed except for those with a frequency of <0.03. Hap1 (-TGGGC-) had the highest haplotype frequencies in LX (27.30%), Hap6(-TGGCC-) had the highest haplotype frequencies in QC (21.70%) and JX (32.30%). Association analysis indicated that P2, P4_2, and P7_4 loci were all significantly associated with growth traits and combined genotype TTGCGC was highly significantly associated with Chest circumference and body weight than the other genotype in JX cattle population. The results of this study suggest that the CaSR gene possibly is a strong candidate gene that affects growth traits in the Chinese cattle breeding program.
authors.affiliation: a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
authors.forename: Binglin
authors.initials: B
authors.lastname: Yue
authors.affiliation: a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
authors.forename: Fuhai
authors.initials: F
authors.lastname: Han
authors.affiliation: a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
authors.forename: Jiyao
authors.initials: J
authors.lastname: Wu
authors.affiliation: a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
authors.forename: Yanhuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
authors.forename: Xingtang
authors.initials: X
authors.lastname: Fang
authors.affiliation: b Bureau of Animal Husbandry of Biyang County , Biyang , Henan , China.
authors.forename: Xinglei
authors.initials: X
authors.lastname: Qi
authors.affiliation: c Animal Health Supervision in Henan Province , Zhengzhou , Henan , China.
authors.forename: Yueyu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D006239:Haplotypes / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D044169:Receptors, Calcium-Sensing / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D044169:Receptors, Calcium-Sensing
keywords: CaSR gene; cattle; combined haplotype; growth traits; sequence variants