title: miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.
abstract: Limited microRNAs (miRNAs, miRs) have been reported to be necessary for exercise-induced cardiac growth and essential for protection against pathological cardiac remodeling. Here we determined members of the miR-17-92 cluster and their passenger miRNAs expressions in two distinct murine exercise models and found that miR-17-3p was increased in both. miR-17-3p promoted cardiomyocyte hypertrophy, proliferation, and survival. TIMP-3 was identified as a direct target gene of miR-17-3p whereas PTEN was indirectly inhibited by miR-17-3p. Inhibition of miR-17-3pin vivoattenuated exercise-induced cardiac growth including cardiomyocyte hypertrophy and expression of markers of myocyte proliferation. Importantly, mice injected with miR-17-3p agomir were protected from adverse remodeling after cardiac ischemia/reperfusion injury. Collectively, these data suggest that miR-17-3p contributes to exercise-induced cardiac growth and protects against adverse ventricular remodeling. miR-17-3p may represent a novel therapeutic target to promote functional recovery after cardiac ischemia/reperfusion.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.affiliation: Cardiac Regeneration and Ageing Lab, School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Bei
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiangqing
authors.initials: X
authors.lastname: Kong
authors.affiliation: Massachusetts General Hospital Cardiovascular Division and Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Experimental Cardiology, University Medical Centre Utrecht, Utrecht 3508GA, The Netherlands.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lei
authors.affiliation: Cardiac Regeneration and Ageing Lab, School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Tianzhao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Qinkao
authors.initials: Q
authors.lastname: Xuan
authors.affiliation: Cardiac Regeneration and Ageing Lab, School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Che
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medical Genomics & Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Jiuchang
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Laboratory of Experimental Cardiology, University Medical Centre Utrecht, Utrecht 3508GA, The Netherlands.
authors.forename: Joost Pg
authors.initials: JP
authors.lastname: Sluijter
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Li
authors.affiliation: Massachusetts General Hospital Cardiovascular Division and Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Rosenzweig
authors.affiliation: Cardiac Regeneration and Ageing Lab, School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D006321:Heart / Q000502:physiology*; D006984:Hypertrophy; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000502:physiology; D010805:Physical Conditioning, Animal; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D035683:MicroRNAs; C543614:Mirn17 microRNA, mouse
keywords: Cardiac growth; Exercise; microRNA.