title: A multistrain approach to studying the mechanisms underlying compatibility in the interaction between Biomphalaria glabrata and Schistosoma mansoni.
abstract: In recent decades, numerous studies have sought to better understand the mechanisms underlying the compatibility between Biomphalaria glabrata and Schistosoma mansoni. The developments of comparative transcriptomics, comparative genomics, interactomics and more targeted approaches have enabled researchers to identify a series of candidate genes. However, no molecular comparative work has yet been performed on multiple populations displaying different levels of compatibility. Here, we seek to fill this gap in the literature. We focused on B. glabrata FREPs and S. mansoni SmPoMucs, which were previously demonstrated to be involved in snail/schistosome compatibility. We studied the expression and polymorphisms of these factors in combinations of snail and schistosome isolates that display different levels of compatibility. We found that the polymorphism and expression levels of FREPs and SmPoMucs could be linked to the compatibility level of S. mansoni. These data and our complementary results obtained by RNA-seq of samples from various snail strains indicate that the mechanism of compatibility is much more complex than previously thought, and that it is likely to be highly variable within and between populations. This complexity must be taken into account if we hope to identify the molecular pathways that are most likely to be good targets for strategies aimed at blocking transmission of the parasite through the snail intermediate host.
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Richard
authors.identifier: 0000-0002-4180-9303
authors.initials: R
authors.lastname: Galinier
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Roger
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Moné
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: David
authors.initials: D
authors.lastname: Duval
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Anaïs
authors.identifier: 0000-0001-7592-0432
authors.initials: A
authors.lastname: Portet
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Silvain
authors.identifier: 0000-0002-9541-3611
authors.initials: S
authors.lastname: Pinaud
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Cristian
authors.identifier: 0000-0002-5162-349X
authors.initials: C
authors.lastname: Chaparro
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Christoph
authors.initials: C
authors.lastname: Grunau
authors.affiliation: MGX-Montpellier GenomiX, Montpellier Genomics and Bioinformatics Facility, Montpellier, France.
authors.forename: Clémence
authors.initials: C
authors.lastname: Genthon
authors.affiliation: MGX-Montpellier GenomiX, Montpellier Genomics and Bioinformatics Facility, Montpellier, France.
authors.forename: Emeric
authors.identifier: 0000-0002-6052-4178
authors.initials: E
authors.lastname: Dubois
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Rognon
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Arancibia
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Dejean
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: André
authors.initials: A
authors.lastname: Théron
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Gourbal
authors.affiliation: Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, Perpignan, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mitta
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics; D001700:Biomphalaria / Q000235:genetics / Q000469:parasitology*; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics; D011110:Polymorphism, Genetic; D012550:Schistosoma mansoni / Q000235:genetics / Q000254:growth & development*; D017422:Sequence Analysis, DNA
chemical_list: D000947:Antigens, Helminth; D007136:Immunoglobulins