title: Effects of human chorionic gonadotropin, estradiol, and progesterone on interleukin-18 expression in human decidual tissues.
abstract: Interleukin (IL)-18 is a proinflammatory cytokine which mediates a myriad of inflammatory responses during pregnancy. Changes in IL-18 levels have been linked to complications during pregnancy. This study was designed to assess the effects of estradiol, human chorionic gonadotropin (hCG), and progesterone on IL-18 expression in human decidual tissues. Uterine deciduas from women with normal pregnancy, spontaneous abortion, and progesterone treated spontaneous abortion were collected, and the effects of hormones on the viability of decidual cells and IL-18 secretion were detected by MTT and ELISA assays. We found that Estradiol, hCG, and progesterone inhibited decidual cell growth independent of dosage and time.IL-18 secretion in the spontaneous abortion group was increased in the decidual cells over time, but all hormones significantly reduced its secretion (p < 0.05). Our results indicate that estradiol, hCG, and progesterone can reduce IL-18 secretion in the cultured endometrial stromal cells from patients who experienced spontaneous abortion to the levels observed following normal pregnancy. Progesterone can significantly reduce IL-18 expression and increase growth of CD56+ CD16- uNK cells, suggesting that these activities may underlie the mechanism by which progesterone improves pregnancy outcomes.
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: b Department of Obstetrics and Gynecology , The People's Hospital of Jiangmen , Guangdong , China , and.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Liu
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Meilian
authors.initials: M
authors.lastname: Deng
authors.affiliation: c Department of Gynecology & Obstetrics , Affiliated Hospital of Yan'an University , Yan'an , China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Xia
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: a Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University , Guangdong , China.
authors.forename: Dunjin
authors.initials: D
authors.lastname: Chen
mesh_terms: D000022:Abortion, Spontaneous / Q000378:metabolism; D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003656:Decidua / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D020382:Interleukin-18 / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D006063:Chorionic Gonadotropin; D020382:Interleukin-18; D011374:Progesterone; D004958:Estradiol
keywords: Interleukin-18; spontaneous abortion; uterine natural killer cell