title: Graphite paper-based bipolar electrode electrochemiluminescence sensing platform.
abstract: In this work, aiming at the construction of a disposable, wireless, low-cost and sensitive system for bioassay, we report a closed bipolar electrode electrochemiluminescence (BPE-ECL) sensing platform based on graphite paper as BPE for the first time. Graphite paper is qualified as BPE due to its unique properties such as excellent electrical conductivity, uniform composition and ease of use. This simple BPE-ECL device was applied to the quantitative analysis of oxidant (H2O2) and biomarker (CEA) respectively, according to the principle of BPE sensing-charge balance. For the H2O2analysis, Pt NPs were electrodeposited onto the cathode through a bipolar electrodeposition approach to promote the sensing performance. As a result, this BPE-ECL device exhibited a wide linear range of 0.001-15mM with a low detection limit of 0.5µM (S/N=3) for H2O2determination. For the determination of CEA, chitosan-multi-walled carbon nanotubes (CS-MWCNTs) were employed to supply a hydrophilic interface for immobilizing primary antibody (Ab1); and Au@Pt nanostructures were conjugated with secondary antibody (Ab2) as catalysts for H2O2reduction. Under the optimal conditions, the BPE-ECL immunodevice showed a wide linear range of 0.01-60ngmL-1with a detection limit of 5.0pgmL-1for CEA. Furthermore, it also displayed satisfactory selectivity, excellent stability and good reproducibility. The developed method opened a new avenue to clinical bioassay.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China. Electronic address: snding@seu.edu.cn.
authors.forename: Shou-Nian
authors.initials: SN
authors.lastname: Ding
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification*; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000302:isolation & purification*; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry; D010209:Paper
chemical_list: D002272:Carcinoembryonic Antigen; D037742:Nanotubes, Carbon; D006046:Gold; D006108:Graphite; D048271:Chitosan; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
keywords: Bipolar electrode; Carcinoembryonic antigen; Electrochemiluminescence; Graphite paper; Immunoassay