title: Impacts of the overexpression of a tomato translationally controlled tumor protein (TCTP) in tobacco revealed by phenotypic and transcriptomic analysis.
abstract: Overexpression of a tomato TCTP impacts plant biomass production and performance under stress. These phenotypic alterations were associated with the up-regulation of genes mainly related to photosynthesis, fatty acid metabolism and water transport. The translationally controlled tumor protein (TCTP) is a multifaceted and highly conserved eukaryotic protein. In plants, despite the existence of functional data implicating this protein in cell proliferation and growth, the detailed physiological roles of many plant TCTPs remain poorly understood. Here we focused on a yet uncharacterized TCTP from tomato (SlTCTP). We show that, when overexpressed in tobacco, SlTCTP may promote plant biomass production and affect performance under salt and osmotic stress. Transcriptomic analysis of the transgenic plants revealed the up-regulation of genes mainly related to photosynthesis, fatty acid metabolism and water transport. This induced photosynthetic gene expression was paralleled by an increase in the photosynthetic rate and stomatal conductance of the transgenic plants. Moreover, the transcriptional modulation of genes involved in ABA-mediated regulation of stomatal movement was detected. On the other hand, genes playing a pivotal role in ethylene biosynthesis were found to be down-regulated in the transgenic lines, thus suggesting deregulated ethylene accumulation in these plants. Overall, these results point to a role of TCTP in photosynthesis and hormone signaling.
authors.affiliation: Departamento de Genética, Instituto de Biociências, UNESP, Botucatu, SP, 18618-970, Brazil.
authors.forename: Márcio
authors.initials: M
authors.lastname: de Carvalho
authors.affiliation: Departamento de Física e Biofísica, Instituto de Biociências, UNESP, Botucatu, SP, Brazil.|Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, 8905, MH 7491, Norway.
authors.forename: Márcio Luís
authors.initials: ML
authors.lastname: Acencio
authors.affiliation: Departamento de Genética, Instituto de Biociências, UNESP, Botucatu, SP, 18618-970, Brazil.
authors.forename: Alessandra Vasconcellos Nunes
authors.initials: AVN
authors.lastname: Laitz
authors.affiliation: Departamento de Genética, Instituto de Biociências, UNESP, Botucatu, SP, 18618-970, Brazil.
authors.forename: Laura Migliorini
authors.initials: LM
authors.lastname: de Araújo
authors.affiliation: Departamento de Genética, Instituto de Biociências, UNESP, Botucatu, SP, 18618-970, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: de Lara Campos Arcuri
authors.affiliation: Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida (LaCTAD), UNICAMP, Campinas, SP, Brazil.
authors.forename: Leandro Costa
authors.initials: LC
authors.lastname: do Nascimento
authors.affiliation: Departamento de Genética, Instituto de Biociências, UNESP, Botucatu, SP, 18618-970, Brazil. igmaia@ibb.unesp.br.
authors.forename: Ivan G
authors.initials: IG
authors.lastname: Maia
mesh_terms: D005030:Ethylenes / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D005030:Ethylenes; D010940:Plant Proteins; C036216:ethylene
keywords: Abiotic stress; RNA-seq; TCTP; Tomato; Translationally controlled tumor protein