title: TNFAIP3 inhibits migration and invasion in nasopharyngeal carcinoma by suppressing epithelial mesenchymal transition.
abstract: TNF alpha induced protein 3 (TNFAIP3), a member of zinc finger protein family, is a gene whose expression level is promptly induced by the tumor necrosis factor. In this study, the clinical significance of TNFAIP3 was analyzed based on available samples in The Cancer Genome Atlas database. TNFAIP3 downregulation was associated with distant metastasis and worse patient prognosis. TNFAIP3-overexpressing and TNFAIP3-knockdown NPC cell line models were established through plasmid-mediated overexpression and small interfering RNA (siRNA), respectively. Cell migration and invasion capacities were evaluated by wound-healing and transwell assays. Functional studies indicated that TNFAIP3 knockdown promoted migration and invasion, whereas TNFAIP3 overexpression alleviated these functions. Western blot analysis was used to examine protein changes from TNFAIP3 overexpression and knockdown, in which TNFAIP3 promoted the protein expression of E-cadherin and suppressed vimentin expression. Our data suggested that TNFAIP3 inhibited migration and invasion by suppressing epithelial mesenchymal transition in NPC.
authors.forename: T
authors.initials: T
authors.lastname: Huang
authors.forename: L
authors.initials: L
authors.lastname: Yin
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: J J
authors.initials: JJ
authors.lastname: Gu
authors.forename: K
authors.initials: K
authors.lastname: Ding
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: M Y
authors.initials: MY
authors.lastname: Du
authors.forename: L X
authors.initials: LX
authors.lastname: Qian
authors.forename: Z W
authors.initials: ZW
authors.lastname: Lu
authors.forename: X
authors.initials: X
authors.lastname: He
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D000077274:Nasopharyngeal Carcinoma / Q000235:genetics*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3 / Q000235:genetics*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D034741:RNA, Small Interfering; C000629650:VIM protein, human; D014746:Vimentin; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3
keywords: TNFAIP3; invasion metastasis.; nasopharyngeal carcinoma