title: Expression of miRNA-155 in carotid atherosclerotic plaques of apolipoprotein E knockout (ApoE-/-) mice and the interventional effect of rapamycin.
abstract: Carotid atherosclerosis (AS) is an inflammatory process and is the primary pathogenesis of cerebrovascular disease. Many factors are responsible for development of atherosclerosis such as inflammation and autophagy. It is reported that microRNAs (miRNAs) could regulate the development of atherosclerosis through targeting autophagy-related genes. Many studies have demonstrated that miRNA-155 could regulate autophagy in macrophages or tumor cells. However, the role of miRNA-155 on autophagy in carotid plaques is not yet known. In this study, we explore the expression of miRNA-155 and autophagy-related proteins in carotid plaques of ApoE-/-mice and the interventional effect of rapamycin. We compared the expression of miRNA-155 and autophagy-related proteins between the control, model and rapamycin groups using qRT-PCR and western blot. Compared to the control group, we found the miRNA-155 and LC3-II expression was up-regulated (P<0.05), expression ratio of phosphorylated mammalian target of rapamycin to total mammalian target of rapamycin (p-mTOR/mTOR) was down-regulated in model group (P<0.05), but atherosclerotic lesions were still aggravated. These results following rapamycin group indicated that miRNA-155 and LC3-II expression was significantly up-regulated (P<0.05), the expression ratio of p-mTOR/mTOR was significantly down-regulated (P<0.05), and atherosclerotic lesions were reduced. Our results showed in the early stages of atherosclerotic plaques development, effective autophagy could attenuate atherosclerosis in ApoE-/-mice. Furthermore, our results also demonstrated that rapamycin might promote the activation of the autophagy by enhancing the expression of miRNA-155, which delays the development of atherosclerotic plaques.
authors.affiliation: Department of Neurology, The Affiliated Hospital of the Qingdao University, Qingdao, Shandong Province 266003, PR China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Neurology, The Affiliated Hospital of the Qingdao University, Qingdao, Shandong Province 266003, PR China.
authors.forename: Shaonan
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Neurology, The Affiliated Hospital of the Qingdao University, Qingdao, Shandong Province 266003, PR China. Electronic address: drmajls@126.com.
authors.forename: Aijun
authors.initials: A
authors.lastname: Ma
authors.affiliation: Department of Neurology, The Affiliated Hospital of the Qingdao University, Qingdao, Shandong Province 266003, PR China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Pan
authors.affiliation: Laboratory of Human Micromorphology, The Medical College of Qingdao University, Qingdao 266003, PR China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Affiliated Hospital of the Qingdao University, Qingdao, Shandong Province 266003, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Central Laboratory, The Affiliated Hospital of the Qingdao University, Qingdao, Shandong Province 266003, PR China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Laboratory of Human Micromorphology, The Medical College of Qingdao University, Qingdao 266003, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D050197:Atherosclerosis / Q000188:drug therapy / Q000235:genetics*; D001343:Autophagy / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D058226:Plaque, Atherosclerotic / Q000188:drug therapy / Q000235:genetics*; D020123:Sirolimus / Q000627:therapeutic use; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D001057:Apolipoproteins E; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus
keywords: Atherosclerosis; Autophagy; Rapamycin; miRNA-155