title: Complement C3 of the innate immune system secreted by muscle adipogenic cells promotes myogenic differentiation.
abstract: Myogenic differentiation results in different cell type cooperation, but the molecules involved in the myogenic cell activation remain elusive. Here, we show that muscle-resident pre-adipocytes promote myogenic differentiation through the secretion of factors. Using proteomic and transcriptomic analyses, we identified that proliferative adipogenic lineage cells produce and secrete a key factor of the innate immune system, the complement C3. Cell culture experiments revealed that C3 promotes the differentiation of myogenic progenitors following internalisation of the immune molecule. These data demonstrate that the third component of the complement system, which is a pivotal factor in the immune response to pathogens, is also involved in the differentiation of myogenic progenitor cells.
authors.affiliation: UFIP UMR-CNRS 6286, Epigénétique: prolifération et différenciation, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Rouaud
authors.affiliation: UFIP UMR-CNRS 6286, Epigénétique: prolifération et différenciation, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France.
authors.forename: Nader
authors.initials: N
authors.lastname: Siami
authors.affiliation: UFIP UMR-CNRS 6286, Epigénétique: prolifération et différenciation, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France.
authors.forename: Tanaelle
authors.initials: T
authors.lastname: Dupas
authors.affiliation: UFIP UMR-CNRS 6286, Epigénétique: prolifération et différenciation, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Gervier
authors.affiliation: UFIP UMR-CNRS 6286, Epigénétique: prolifération et différenciation, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France.
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Gardahaut
authors.affiliation: UFIP UMR-CNRS 6286, Epigénétique: prolifération et différenciation, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France. Gwenola.Boucher@univ-nantes.fr.
authors.forename: Gwenola
authors.initials: G
authors.lastname: Auda-Boucher
authors.affiliation: UFIP UMR-CNRS 6286, Epigénétique: prolifération et différenciation, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France. Christophe.Thiriet@univ-nantes.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Thiriet
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003176:Complement C3 / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D051379:Mice; D032342:Muscle Cells / Q000166:cytology / Q000378:metabolism; D024510:Muscle Development; D040901:Proteomics; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: C545610:C3 protein, mouse; D003176:Complement C3