title: Detection of β-agonists in pork tissue with novel electrospun nanofibers-based solid-phase extraction followed ultra-high performance liquid chromatography/tandem mass spectrometry.
abstract: A selective analytical method based on packed-fiber solid-phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (PFSPE-UPLC-MS/MS) has been developed for determination of six β-agonists (clorprenaline, bambuterol, clenbuterol, brombuterol, mabuterol, and penbuterol) in pork tissue. Polystyrene-polymeric crown ether (PS-PCE) composite nanofibers were fabricated by electrospinning and utilized to prepare the homemade extraction columns. With optimal conditions, all analytes were separated very well and the blank pork did not disturb the determination, and the linearity is good in a range of 5.0μg/kg-25.0μg/kg. The recoveries were 79.3-110.1%. RSDs for intra-day were in the range of 1.5-10.5% and RSDs for inter-day were 4.7-11.8%. Above all, only 5mg of sorbent and 200μL of elution solvent were favorable to directly extract all analytes in a complex matrix. The method is simple and cost-effective, and has the potential to be applied to quantitatively analyze the concentrations of polar species in food samples containing complex matrix.
authors.affiliation: Key Laboratory of Environmental Medicine and Engineering (Ministry of Education), School of Public Health, Southeast University, Nanjing 210096, China.
authors.forename: Lanling
authors.initials: L
authors.lastname: Chu
authors.affiliation: Animal Products Quality Inspection and Test Center in Jiangsu Province, Nanjing 210036, China.
authors.forename: Shenglan
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Animal Products Quality Inspection and Test Center in Jiangsu Province, Nanjing 210036, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qu
authors.affiliation: Animal Products Quality Inspection and Test Center in Jiangsu Province, Nanjing 210036, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Geng
authors.affiliation: Key Laboratory of Environmental Medicine and Engineering (Ministry of Education), School of Public Health, Southeast University, Nanjing 210096, China; Key Laboratory of Child Development and Learning Science (Ministry of Education), Research Centre for Learning Science, Southeast University, Nanjing 210096, China. Electronic address: xjkang64@163.com.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Kang
mesh_terms: D000318:Adrenergic beta-Agonists / Q000737:chemistry* / Q000302:isolation & purification*; D000814:Aniline Compounds / Q000032:analysis / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002976:Clenbuterol / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D004350:Drug Residues / Q000737:chemistry / Q000302:isolation & purification; D004983:Ethanolamines / Q000032:analysis / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D057139:Nanofibers / Q000032:analysis; D011108:Polymers / Q000032:analysis; D011137:Polystyrenes / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000318:Adrenergic beta-Agonists; D000814:Aniline Compounds; D004983:Ethanolamines; D011108:Polymers; D011137:Polystyrenes; D019155:Veterinary Drugs; C107573:brombuterol; C044573:mabuterol; D002976:Clenbuterol
keywords: Nanofiber solid-phase extraction; Pork tissue; Ultra-high performance liquid chromatography/tandem mass spectrometry; β-Agonists