title: Towards understanding cellular structure biology: In-cell NMR.
abstract: To watch biological macromolecules perform their functions inside the living cells is the dream of any biologists. In-cell nuclear magnetic resonance is a branch of biomolecular NMR spectroscopy that can be used to observe the structures, interactions and dynamics of these molecules in the living cells at atomic level. In principle, in-cell NMR can be applied to different cellular systems to achieve biologically relevant structural and functional information. In this review, we summarize the existing approaches in this field and discuss its applications in protein interactions, folding, stability and post-translational modifications. We hope this review will emphasize the effectiveness of in-cell NMR for studies of intricate biological processes and for structural analysis in cellular environments.
authors.affiliation: Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, South Korea.
authors.forename: Safikur
authors.initials: S
authors.lastname: Rahman
authors.affiliation: Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, South Korea.
authors.forename: Younhwa
authors.initials: Y
authors.lastname: Byun
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 10025, India.
authors.forename: Md Imtaiyaz
authors.initials: MI
authors.lastname: Hassan
authors.affiliation: Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, South Korea. Electronic address: kimjihoe@ynu.ac.kr.
authors.forename: Jihoe
authors.initials: J
authors.lastname: Kim
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 10025, India. Electronic address: vijay9595@st.jmi.ac.in.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
mesh_terms: D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D017510:Protein Folding; D025941:Protein Interaction Mapping / Q000379:methods*; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D046911:Macromolecular Substances; D011506:Proteins
keywords: In-cell NMR; Protein maturation; Protein stability; Protein-protein interactions; Structure biology; α-Synuclein