title: Identification and production of bioflocculants by Enterobacter sp. and Bacillus sp. and their characterization studies.
abstract: In this work, two bioflocculants, namely, EB-EPS and B1-EPS, were derived from Enterobacter sp. and Bacillus sp., respectively, and analyzed with regard to their production and characterization. About 0.9 and 0.16 g of purified EB and B1 were obtained from I L of fermentation broth. Chemical analysis showed the contents of purified EB and B1 mainly as 88.7 and 92.8% (w/w) of carbohydrate, and 11.3 and 21.8% (w/w) protein, respectively. Fourier-transform infrared spectrometry analysis revealed the presence of hydroxyl, amide, and carboxyl groups in the identified bioflocculant. Thermogravimetric analysis (TGA) results exhibited enhanced thermal stability with a minimum mass loss of 50% while 25% were found to have occurred at higher temperatures (>400°C) for microbe-derived compounds EB and B1 leading to the possibility of using these compounds as fillers or for fabricating composite films for high-temperature applications. Further, the compounds from both the bacteria exhibited good antibacterial characteristics against pathogenic Escherichia coli. Degradability study of bioflocculant-embedded composite films shows the possibility of attaining eco-friendly bioremediation. Accordingly, experimental results revealed the suitability of developed composite films as a suitable alternative for food packaging and biomedical applications.
authors.affiliation: a Department of Biotechnology , Kalasalingam University , Virudhunagar , Tamilnadu , India.
authors.forename: L
authors.initials: L
authors.lastname: Muthulakshmi
authors.affiliation: b Research and Development Department , Bangalore Biotech Labs (BiOZEEN) , Bangalore , India.
authors.forename: H
authors.initials: H
authors.lastname: Nellaiah
authors.affiliation: a Department of Biotechnology , Kalasalingam University , Virudhunagar , Tamilnadu , India.
authors.forename: T
authors.initials: T
authors.lastname: Kathiresan
authors.affiliation: c Department of Mechanical Engineering , Kalasalingam University , Virudhunagar , Tamilnadu , India.
authors.forename: N
authors.initials: N
authors.lastname: Rajini
authors.affiliation: d Department of Mechanical Engineering , University of West Hungary , Szombathely , Hungary.
authors.forename: Fenila
authors.initials: F
authors.lastname: Christopher
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001704:Biopolymers / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004754:Enterobacter / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy; D005285:Fermentation; D005427:Flocculation; D006801:Humans; D007218:Industrial Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001704:Biopolymers
keywords: Antibacterial; TGA; differential scanning calorimetry; microbe-derived bioflocculant