title: Untangling the transcription regulatory network of the bacitracin synthase operon in Bacillus licheniformis DW2.
abstract: The bacitracin synthetase gene cluster in Bacillus licheniformis DW2 is composed of the bacABC operon encoding a non-ribosomal peptide synthetase and bacT encoding a thioesterase. Although the bacitracin gene cluster has been well studied, little is known about how this gene cluster is regulated. This study provides insight into how the transcription factors Spo0A and AbrB regulate bacitracin biosynthesis. Deletion of spo0A resulted in drastically reduced expression of bacA and bacT, and subsequently bacitracin production. On the other hand, the expression of bacA and bacT increased significantly in B. licheniformis DW2ΔabrB and DW2Δ0AΔabrB compared to the wild-type strain DW2. The bacitracin yields on cell numbers (U/CFU) in DW2ΔabrB and DW2Δ0A/pHY300-0A-sad67 were 17.5% and 14.9% higher than that of the wild-type strain. An electrophoretic mobility shift assay (EMSA) further confirmed that AbrB could directly bind to the promoter regions of bacA and bacT. These results indicate that AbrB acts as a repressor of bacitracin biosynthesis by inhibiting bacA and bacT expression, while Spo0A indirectly promotes bacitracin biosynthesis by repressing abrB expression.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China; Department of Chemistry, State University of New York-College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Nomura
authors.affiliation: Lifecome Biochemistry Stock CO. Ltd., Pucheng 353400, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: mel212@126.com.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Chen
mesh_terms: D000069976:Bacillus licheniformis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001414:Bacitracin / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks; D009097:Multienzyme Complexes / Q000235:genetics*; D009876:Operon; D010453:Peptide Synthases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D009097:Multienzyme Complexes; D014157:Transcription Factors; D001414:Bacitracin; D010453:Peptide Synthases; C019761:bacitracin synthetase
keywords: AbrB; Bacillus licheniformis; Bacitracin; Spo0A; Transcription regulation