title: Identification and characterization of the bombykal receptor in the hawkmothManduca sexta.
abstract: Manduca sextafemales attract their mates with the release of a species-specific sex-pheromone blend, with bombykal (E,Z)-10,12-hexadecadienal and (E,E,Z)-10,12,14-hexadecatrienal being the two major components. Here, we searched for the hawkmoth bombykal receptor in heterologous expression systems. The putative pheromone receptor MsexOr1 coexpressed with MsexOrco inXenopusoocytes elicited dose-dependent inward currents upon bombykal application (10-300 μmol l-1), and coexpressed in HEK293 and CHO cells caused bombykal-dependent increases in the intracellular free Ca2+concentration. In addition, the bombykal receptor ofBombyx moriBmOr3 coexpressed with MsexOrco responded to bombykal (30-100 μmol l-1) with inward currents. In contrast, MsexOr4 coexpressed with MsexOrco responded neither to bombykal (30-100 μmol l-1) nor to the (E,E,Z)-10,12,14-hexadecatrienal mimic. Thus, MsexOr1, but not MsexOrco and probably not MsexOr4, is the bombykal-binding pheromone receptor in the hawkmoth. Finally, we obtained evidence that phospholipase C and protein kinase C activity are involved in the hawkmoth's bombykal-receptor-mediated Ca2+signals in HEK293 and CHO cells.
authors.affiliation: Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, 07745 Jena, Germany dwicher@ice.mpg.de.
authors.forename: Dieter
authors.identifier: 0000-0001-6443-3399
authors.initials: D
authors.lastname: Wicher
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo and JST ERATO Touhara Chemosensory Signal Project, Tokyo 113-8657, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morinaga
authors.affiliation: Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, 07745 Jena, Germany.
authors.forename: Lorena
authors.initials: L
authors.lastname: Halty-deLeon
authors.affiliation: University of Kassel, Biology, Animal Physiology, 34132 Kassel, Germany.
authors.forename: Nico
authors.initials: N
authors.lastname: Funk
authors.affiliation: Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, 07745 Jena, Germany.
authors.forename: Bill
authors.initials: B
authors.lastname: Hansson
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo and JST ERATO Touhara Chemosensory Signal Project, Tokyo 113-8657, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Touhara
authors.affiliation: University of Kassel, Biology, Animal Physiology, 34132 Kassel, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Stengl
mesh_terms: D000466:Alkadienes / Q000494:pharmacology; D000818:Animals; D012831:Bombyx; D020013:Calcium Signaling; D003412:Cricetulus; D057809:HEK293 Cells; D006801:Humans; D018402:Manduca / Q000166:cytology / Q000502:physiology*; D018034:Olfactory Receptor Neurons; D009865:Oocytes; D018035:Receptors, Odorant; D012724:Sex Attractants / Q000494:pharmacology*; D014981:Xenopus
chemical_list: D000466:Alkadienes; D018035:Receptors, Odorant; D012724:Sex Attractants; C066482:bombykal
keywords: Manduca sexta; Modulation; Orco; PKC; PLC; Pheromone receptor