title: Oxidative status and prolidase activities in generalized anxiety disorder.
abstract: OBJECTIVE
  Prolidase (Pro), an intracellular enzyme necessary for collagen turnover, matrix remodelling and cell growth has been shown to be related to Oxidative Stress (OS). To our knowledge, serum Pro activity in generalized anxiety disorder (GAD) has not been documented yet. In this study, we aimed to evaluate OS and its relation with Pro activity in patients diagnosed with GAD.
  
  
  METHODS
  Thirty untreated GAD patients and 30 healthy controls were included in the study. Blood samples were collected from all subjects to quantify total oxidant status (TOS), total antioxidant status (TAS) and Pro activity. Oxidative stress index (OSI), the ratio of TOS to TAS, is calculated to evaluate the balance between antioxidants and oxidants. Hamilton Anxiety Rating Scale (HARS) was used to determine the anxiety levels of all subjects.
  
  
  RESULTS
  GAD group demonstrated statistically significantly higher TOS, OSI and Pro levels, when compared with the control group (t=2.947, p=0.005; t=2.874, p=0.006; and t=9.396, p<0.001 respectively). HARS scores were found to be positively correlated with TOS, OSI and Pro levels (p=0.008, r=0.338; p=0.008, r=0.339; and p<0.001, r=0.751 respectively).
  
  
  CONCLUSIONS
  The degree of severity of OS is correlated with the levels of Pro. Thus, Pro might be the target enzyme, promising to be a marker for the follow-up of GAD patients. To the best of our knowledge, this study is the first to report a significant relation between Pro activity and GAD.
authors.affiliation: Department of Psychiatry, Recep Tayyip Erdogan University Faculty of Medicine, Rize Research and Training Hospital. Rize Egitim ve Arastirma Hastanesi Psikiyatri Poliklinigi 53020 Rize, Turkey. Electronic address: acenkercan@gmail.com.
authors.forename: A Cenk
authors.initials: AC
authors.lastname: Ercan
authors.affiliation: Department of Psychiatry, Recep Tayyip Erdogan University Faculty of Medicine, Rize Research and Training Hospital. Rize Egitim ve Arastirma Hastanesi Psikiyatri Poliklinigi 53020 Rize, Turkey. Electronic address: bulentbahceci@hotmail.com.
authors.forename: Bulent
authors.initials: B
authors.lastname: Bahceci
authors.affiliation: Department of Psychiatry, Recep Tayyip Erdogan University Faculty of Medicine, Rize Research and Training Hospital. Rize Egitim ve Arastirma Hastanesi Psikiyatri Poliklinigi 53020 Rize, Turkey. Electronic address: md.selim@hotmail.com.
authors.forename: Selim
authors.initials: S
authors.lastname: Polat
authors.affiliation: Department of Psychiatry, Rize Kackar State Hospital. Kackar Devlet Hastanesi Psikiyatri Poliklinigi 53340 Pazar Rize, Turkey. Electronic address: ozgurcaala@gmail.com.
authors.forename: Ozgur Cagla
authors.initials: OC
authors.lastname: Cenker
authors.affiliation: Department of Medical Microbiology, Recep Tayyip Erdogan University Faculty of Medicine, Rize Research and Training Hospital. Rize Egitim ve Arastirma Hastanesi Medikal Mikrobiyoloji Laboratuvarı 53020 Rize, Turkey. Electronic address: bahceciie@hotmail.com.
authors.forename: Ilkay
authors.initials: I
authors.lastname: Bahceci
authors.affiliation: Department of Psychiatry, Recep Tayyip Erdogan University Faculty of Medicine, Rize Research and Training Hospital. Rize Egitim ve Arastirma Hastanesi Psikiyatri Poliklinigi 53020 Rize, Turkey. Electronic address: dr.clue@hotmail.com.
authors.forename: Ayse
authors.initials: A
authors.lastname: Koroglu
authors.affiliation: Department of Medical Microbiology, Recep Tayyip Erdogan University Faculty of Medicine, Rize Research and Training Hospital. Rize Egitim ve Arastirma Hastanesi Medikal Mikrobiyoloji Laboratuvarı 53020 Rize, Turkey. Electronic address: ksahin3@mynet.com.
authors.forename: Kazim
authors.initials: K
authors.lastname: Sahin
authors.affiliation: Department of Psychiatry, Recep Tayyip Erdogan University Faculty of Medicine, Rize Research and Training Hospital. Rize Egitim ve Arastirma Hastanesi Psikiyatri Poliklinigi 53020 Rize, Turkey. Electronic address: cicekh@gmail.com.
authors.forename: Cicek
authors.initials: C
authors.lastname: Hocaoglu
mesh_terms: D000328:Adult; D001008:Anxiety Disorders / Q000201:enzymology*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004150:Dipeptidases / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004150:Dipeptidases; C022449:proline dipeptidase
keywords: Generalized anxiety disorder; Oxidative stress; Prolidase; Total anxiety disorder; Total oxidant status