title: Effect of florfenicol on performance and microbial community of a sequencing batch biofilm reactor treating mariculture wastewater.
abstract: The effects of florfenicol (FF) on the performance, microbial activity and microbial community of a sequencing batch biofilm reactor (SBBR) were evaluated in treating mariculture wastewater. The chemical oxygen demand (COD) and nitrogen removal were inhibited at high FF concentrations. The specific oxygen utilization rate (SOUR), specific ammonium oxidation rate (SAOR), specific nitrite oxidation rate (SNOR) and specific nitrate reduction rate (SNRR) were decreased with an increase in the FF concentration from 0 to 35 mg/L. The chemical compositions of loosely bound extracellular polymeric substances (LB-EPS) and tightly bound EPS (TB-EPS) could be affected with an increase in the FF concentration. The high-throughput sequencing indicated some obvious variations in the microbial community at different FF concentrations. The relative abundance of Nitrosomonas and Nitrospira showed a decreasing tendency with an increase in the FF concentration, suggesting that FF could affect the nitrification process of SBBR. Some genera capable of reducing nitrate to nitrogen gas could be inhibited by the addition of FF in the influent, such as Azospirillum and Hyphomicrobium.
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.|b College of Environmental Science and Engineering , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Feng
authors.identifier: http://orcid.org/0000-0003-2566-4850
authors.initials: F
authors.lastname: Gao
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.|b College of Environmental Science and Engineering , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Zhiwei
authors.identifier: http://orcid.org/0000-0002-6037-3269
authors.initials: Z
authors.lastname: Li
authors.affiliation: b College of Environmental Science and Engineering , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Chang
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.|b College of Environmental Science and Engineering , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Mengchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Zonglian
authors.initials: Z
authors.lastname: She
authors.affiliation: c College of Resource and Environment , Qingdao Agricultural University , Qingdao , People's Republic of China.
authors.forename: Juan
authors.identifier: http://orcid.org/0000-0002-9165-8224
authors.initials: J
authors.lastname: Wu
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Chunji
authors.initials: C
authors.lastname: Jin
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.|b College of Environmental Science and Engineering , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Yangguo
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: a Key Lab of Marine Environment and Ecology, Ministry of Education , Ocean University of China , Qingdao , People's Republic of China.|b College of Environmental Science and Engineering , Ocean University of China , Qingdao , People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000633:toxicity; D017756:Aquaculture; D018441:Biofilms / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis / Q000633:toxicity; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D013839:Thiamphenicol; D062065:Wastewater; D014874:Water Pollutants, Chemical; C035534:florfenicol
keywords: Florfenicol; SBBR; extracellular polymeric substances; microbial activity; microbial community