title: Isolation and Structure Identification of Novel Brominated Diketopiperazines from Nocardia ignorata-A Lichen-Associated Actinobacterium.
abstract: Actinobacteria are well known for their potential in biotechnology and their production of metabolites of interest. Lichens are a promising source of new bacterial strains, especially Actinobacteria, which afford a broad chemical diversity. In this context, the culture medium of the actinobacteriumNocardia ignorata,isolated from the terrestrial lichenCollema auriforme, was studied. The strain was cultivated in a BioFlo 115 bioreactor, and the culture medium was extracted using an XAD7HP resin. Five known diketopiperazines: cyclo (l-Pro-l-OMet) (1), cyclo (l-Pro-l-Tyr) (2), cyclo (d-Pro-l-Tyr) (3), cyclo (l-Pro-l-Val) (4), cyclo (l-Pro-l-Leu) (5), and one auxin derivative: indole-carboxaldehyde (8) were isolated, along with two new brominated diketopiperazines: cyclo (d-Pro-l-Br-Tyr) (6) and cyclo (l-Pro-l-Br-Tyr) (7). Structure elucidation was performed using HRMS and 1D and 2D NMR analysis, and the synthesis of compounds6and7was carried out in order to confirm their structure.
authors.affiliation: CORINT, UMR CNRS ISCR 6226, UFR Sciences Pharmaceutiques et Biologiques, Université Bretagne Loire, 2 Av. du Professeur Léon Bernard, 35043 Rennes, France. alba.noel@univ-rennes1.fr.
authors.forename: Alba
authors.initials: A
authors.lastname: Noël
authors.affiliation: CORINT, UMR CNRS ISCR 6226, UFR Sciences Pharmaceutiques et Biologiques, Université Bretagne Loire, 2 Av. du Professeur Léon Bernard, 35043 Rennes, France. solenn.ferron@univ-rennes1.fr.
authors.forename: Solenn
authors.initials: S
authors.lastname: Ferron
authors.affiliation: CORINT, UMR CNRS ISCR 6226, UFR Sciences Pharmaceutiques et Biologiques, Université Bretagne Loire, 2 Av. du Professeur Léon Bernard, 35043 Rennes, France. isabelle.rouaud@univ-rennes1.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Rouaud
authors.affiliation: CORINT, UMR CNRS ISCR 6226, UFR Sciences Pharmaceutiques et Biologiques, Université Bretagne Loire, 2 Av. du Professeur Léon Bernard, 35043 Rennes, France. nicolas.gouault@univ-rennes1.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gouault
authors.affiliation: CORINT, UMR CNRS ISCR 6226, UFR Sciences Pharmaceutiques et Biologiques, Université Bretagne Loire, 2 Av. du Professeur Léon Bernard, 35043 Rennes, France. jean-pierre.hurvois@univ-rennes1.fr.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Hurvois
authors.affiliation: CORINT, UMR CNRS ISCR 6226, UFR Sciences Pharmaceutiques et Biologiques, Université Bretagne Loire, 2 Av. du Professeur Léon Bernard, 35043 Rennes, France. sophie.tomasi@univ-rennes1.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Tomasi
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry; D054659:Diketopiperazines / Q000138:chemical synthesis / Q000737:chemistry*; D008011:Lichens / Q000382:microbiology*; D015394:Molecular Structure; D009615:Nocardia / Q000737:chemistry / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D054659:Diketopiperazines
keywords: Nocardia; diketopiperazines; lichen-associated actinobacterium