title: DNA Fountain enables a robust and efficient storage architecture.
abstract: DNA is an attractive medium to store digital information. Here we report a storage strategy, called DNA Fountain, that is highly robust and approaches the information capacity per nucleotide. Using our approach, we stored a full computer operating system, movie, and other files with a total of 2.14 × 106bytes in DNA oligonucleotides and perfectly retrieved the information from a sequencing coverage equivalent to a single tile of Illumina sequencing. We also tested a process that can allow 2.18 × 1015retrievals using the original DNA sample and were able to perfectly decode the data. Finally, we explored the limit of our architecture in terms of bytes per molecule and obtained a perfect retrieval from a density of 215 petabytes per gram of DNA, orders of magnitude higher than previous reports.
authors.affiliation: New York Genome Center, New York, NY 10013, USA. yaniv@cs.columbia.edu.|Department of Computer Science, Fu Foundation School of Engineering, Columbia University, New York, NY 10027, USA.|Center for Computational Biology and Bioinformatics (C2B2), Department of Systems Biology, Columbia University, New York, NY 10027, USA.
authors.forename: Yaniv
authors.identifier: 0000-0003-3257-8387
authors.initials: Y
authors.lastname: Erlich
authors.affiliation: New York Genome Center, New York, NY 10013, USA.
authors.forename: Dina
authors.identifier: 0000-0003-1357-4233
authors.initials: D
authors.lastname: Zielinski
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D059014:High-Throughput Nucleotide Sequencing; D016247:Information Storage and Retrieval / Q000379:methods*; D046609:Libraries, Digital; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D004247:DNA