title: The expression of asparaginyl endopeptidase promotes growth potential in epithelial ovarian cancer.
abstract: Epithelial ovarian cancer (EOC) is the most common and lethal cancer-related death among females in the world. Asparaginyl endopeptidase (AEP) is a member of C13 family peptidases and expressed in the extracellular matrix and tumor cells. The aim of this article is to explore the function of asparaginyl endopeptidase in epithelial ovarian cancer. The expression of AEP was examined in 20 EOC samples, 3 EOC metastasis samples, 6 fallopian tube metastasis samples, 4 peritoneum metastasis samples and 20 benign ovarian tumor samples by immunohistochemistry. The expression of AEP was also evaluated in serum and ascites of EOC patients by elisa. And we used a lentiviral vector to overexpress AEP in human epithelial ovarian cancer cell lines SKOV3ip and detected the function of AEP-SKOV3ip cells both in vitro and in vivo. The growth of AEP-SKOV3ip cells was observed by MTT, migration and tube formation assays in vitro. Additionally, the subcutaneous mice model was used to identify the tumor growth and metastasis in vivo. Mice tumors were stained for CD31 to determine the microvessel density (MVD). We demonstrated that AEP was highly expressed in the EOC patient tissues and ascites. The AEP transfected SKOV3ip cells could both promote tumor growth in vitro and in vivo. The MVD in AEP-SKOV3ip group was higher than that in NC-SKOV3ip group. Therefore, our results demonstrated that AEP could induce EOC growth and progressionboth in vitro and in vivo.
authors.affiliation: a Department of Gynecology , Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine , Shanghai , P.R. China.
authors.forename: Qinyi
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: a Department of Gynecology , Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine , Shanghai , P.R. China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Tang
authors.affiliation: a Department of Gynecology , Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine , Shanghai , P.R. China.
authors.forename: Xipeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001201:Ascites / Q000473:pathology; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D003546:Cysteine Endopeptidases / Q000097:blood / Q000378:metabolism*; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005185:Fallopian Tube Neoplasms / Q000097:blood / Q000473:pathology* / Q000556:secondary; D005187:Fallopian Tubes / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000097:blood / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000097:blood / Q000473:pathology* / Q000556:secondary; D023041:Xenograft Model Antitumor Assays; D000099240:Asparaginyl Endopeptidase
chemical_list: D003546:Cysteine Endopeptidases; D000099240:Asparaginyl Endopeptidase
keywords: Animal model; asparaginyl endopeptidase; epithelial ovarian cancer; microvessel density