title: Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light.
abstract: Significant progress in characterization of nanoparticles and biomolecules was enabled by the development of advanced imaging equipment with extreme spatial-resolution and sensitivity. To perform some of these analyses outside of well-resourced laboratories, it is necessary to create robust and cost-effective alternatives to existing high-end laboratory-bound imaging and sensing equipment. Towards this aim, we have designed a holographic on-chip microscope operating at an ultraviolet illumination wavelength (UV) of 266 nm. The increased forward scattering from nanoscale objects at this short wavelength has enabled us to detect individual sub-30 nm nanoparticles over a large field-of-view of >16 mm2using an on-chip imaging platform, where the sample is placed at ≤0.5 mm away from the active area of an opto-electronic sensor-array, without any lenses in between. The strong absorption of this UV wavelength by biomolecules including nucleic acids and proteins has further enabled high-contrast imaging of nanoscopic aggregates of biomolecules, e.g., of enzyme Cu/Zn-superoxide dismutase, abnormal aggregation of which is linked to amyotrophic lateral sclerosis (ALS) - a fatal neurodegenerative disease. This UV-based wide-field computational imaging platform could be valuable for numerous applications in biomedical sciences and environmental monitoring, including disease diagnostics, viral load measurements as well as air- and water-quality assessment.
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA, 90095, USA.|Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.|California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA.
authors.forename: Mustafa Ugur
authors.initials: MU
authors.lastname: Daloglu
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA, 90095, USA.|Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.|California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA.
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Ray
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA, 90095, USA.|Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.|California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Gorocs
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA, 90095, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Xiong
authors.affiliation: Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
authors.forename: Ravinder
authors.initials: R
authors.lastname: Malik
authors.affiliation: Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.|Brain Research Institute, University of California, Los Angeles, CA, 90095, USA.|Molecular Biology Institute, University of California, Los Angeles, CA, 90095, USA.
authors.forename: Gal
authors.initials: G
authors.lastname: Bitan
authors.affiliation: College of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Euan
authors.initials: E
authors.lastname: McLeod
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA, 90095, USA.|Bioengineering Department, University of California, Los Angeles, CA, 90095, USA.|California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA.|Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
authors.forename: Aydogan
authors.initials: A
authors.lastname: Ozcan
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000201:enzymology / Q000473:pathology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008860:Microscopy, Ultraviolet / Q000295:instrumentation / Q000379:methods; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D013482:Superoxide Dismutase