title: Tracking Perfluorocarbon Nanoemulsion Delivery by19F MRI for Precise High Intensity Focused Ultrasound Tumor Ablation.
abstract: Perfluorocarbon nanoemulsions (PFCNEs) have recently been undergoing rigorous study to investigate their ability to improve the therapeutic efficacy of tumor ablation by high intensity focused ultrasound (HIFU). For precise control of PFCNE delivery and thermal ablation, their accumulation and distribution in a tumor should be quantitatively analyzed. Here, we used fluorine-19 (19F) magnetic resonance imaging (MRI) to quantitatively track PFCNE accumulation in a tumor, and analyzed how intra-tumoral PFCNE quantities affect the therapeutic efficacy of HIFU treatment. Ablation outcomes were assessed by intra-voxel incoherent motion analysis and bioluminescent imaging up to 14 days after the procedure. Assessment of PFCNE delivery and treatment outcomes showed that 2-3 mg/mL of PFCNE in a tumor produces the largest ablation volume under the same HIFU insonation conditions. Histology showed varying degrees of necrosis depending on the amount of PFCNE delivered.19F MRI promises to be a valuable platform for precisely guiding PFCNE-enhanced HIFU ablation of tumors.
authors.affiliation: Molecular Imaging & Therapy Branch, Division of Precision Medicine, National Cancer Center, Goyang, Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Dept. of Radiology, Seoul National University Hospital, Seoul, Korea.
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Park
authors.affiliation: Molecular Imaging & Therapy Branch, Division of Precision Medicine, National Cancer Center, Goyang, Korea.
authors.forename: Changki
authors.initials: C
authors.lastname: Min
authors.affiliation: Molecular Imaging & Therapy Branch, Division of Precision Medicine, National Cancer Center, Goyang, Korea.
authors.forename: Sun Il
authors.initials: SI
authors.lastname: Choi
authors.affiliation: Molecular Imaging & Therapy Branch, Division of Precision Medicine, National Cancer Center, Goyang, Korea.
authors.forename: Soyeon
authors.initials: S
authors.lastname: Jeon
authors.affiliation: Molecular Imaging & Therapy Branch, Division of Precision Medicine, National Cancer Center, Goyang, Korea.
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Molecular Imaging & Therapy Branch, Division of Precision Medicine, National Cancer Center, Goyang, Korea.
authors.forename: Daehong
authors.initials: D
authors.lastname: Kim
mesh_terms: D055011:Ablation Techniques / Q000379:methods*; D000818:Animals; D002277:Carcinoma / Q000000981:diagnostic imaging* / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D004655:Emulsions / Q000008:administration & dosage; D066235:Fluorine-19 Magnetic Resonance Imaging / Q000379:methods*; D005466:Fluorocarbons / Q000008:administration & dosage*; D006651:Histocytochemistry; D006979:Hyperthermia, Induced / Q000379:methods*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013010:Sonication / Q000379:methods*; D014183:Transplantation, Heterologous; D016896:Treatment Outcome
chemical_list: D004655:Emulsions; D005466:Fluorocarbons
keywords: 19F MRI; Diffusion MR; High Intensity Focused Ultrasound (HIFU); Perfluorocarbon nanoemulsion (PFCNE); Tumor Ablation