title: Protein adsorption of dialdehyde cellulose-crosslinked chitosan with high amino group contents.
abstract: Crosslinked chitosan was prepared by Schiff base formation between the aldehyde groups of dialdehyde cellulose (DAC) and the amino groups of chitosan and a subsequent reduction. DAC was obtained through periodate oxidation of cellulose and solubilization in hot water at 100°C for 1h. Three grades of DAC-crosslinked chitosan were prepared by adding various amounts DAC. The degrees of crosslinking as determined by amino group content were 3.8, 8.3, and 12.1%, respectively. DAC-crosslinked chitosan showed higher stability in the pH 2-9 range and no cytotoxicity was identified over the course of a 21-day long-term stability test. Also, DAC-crosslinked chitosan showed remarkably high bovine serum albumin (BSA) adsorption capacity at pH 5.5 as a result of the increased amino group content, due to the reaction between DAC and chitosan molecular chains occurring at multiple points even though DAC-crosslinked chitosan showed a lower degree of crosslinking.
authors.affiliation: Department of Plant & Environmental New Resources, College of Life Sciences, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea. Electronic address: sbpujkim@khu.ac.kr.
authors.forename: Ung-Jin
authors.initials: UJ
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Republic of Korea.
authors.forename: Yeong Ro
authors.initials: YR
authors.lastname: Lee
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Republic of Korea.
authors.forename: Tong Ho
authors.initials: TH
authors.lastname: Kang
authors.affiliation: Department of Forest Sciences and Research Institute for Agriculture and Life Science, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-921, Republic of Korea.
authors.forename: Joon Weon
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Department of Plant & Environmental New Resources, College of Life Sciences, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea; Department of Biomaterials Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Department of Plant & Environmental New Resources, College of Life Sciences, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea; Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan. Electronic address: wadam@kais.kyoto-u.ac.jp.
authors.forename: Masahisa
authors.initials: M
authors.lastname: Wada
mesh_terms: D000327:Adsorption; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D012710:Serum Albumin, Bovine; D002482:Cellulose; D048271:Chitosan; C058684:2,3-dialdehydocellulose
keywords: Chitosan; Crosslinking; Cytotoxicity; Dialdehyde cellulose; Protein adsorption