title: [Effects of microRNA-373 on the proliferation and invasiveness of breast carcinoma and its mechanisms].
abstract: Objective:To explore the effects of microRNA (miR)-373 on proliferation and invasion of breast cancer cells and its mechanisms.Methods:Breast carcinoma tissues and its corresponding normal tissues (80 cases) were obtained from the First Affiliated Hospital of Zhengzhou University from 2010 to 2014. Quantitative real-time (RT)-PCR was used to detect the expression of miR-373 of breast carcinoma tissues and its corresponding normal tissues. The cellula experiment was divided into three groups: the normal control group, the miR-373 mimic group and the miR-373 inhibitor group. The proliferation was detected by Cell Counting Kit.8 (CCK-8) method and the invasion and migration were detected by Transwell method. Effect of miR-373 on cell apoptosis was determined by Flow Cytometry.Results:Expression of miR-373 in breast cancer tissue was significant lower than the normal breast tissues (0.21±0.09 vs 0.98±0.23,P<0.001); expression of miR-373 contributed to the inhibition of Bcl-6 expression. The apoptosis rate in miR-373 was higher than normal control group (18.29%±0.29% vs 4.91%±0.31%), cell proliferation, cell invasion and cell migration were lower than normal control group [(0.69±0.11) vs (1.25±0.12) (the fifth day), (30.50±0.20) vs (66.50±0.42), (29.50±0.21) vs (48.50±0.31), allP<0.05].Conclusion:miR-373 plays a suppressive role in proliferation and invasion of breast carcinoma, and the downregulation of miR-373 promotes the invasion of breast cancer which may mediate by the increased expression of Bcl-6.
authors.affiliation: Department of Clinical Laboratory Medicine, Key Clinical Laboratory of Heman Province, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Qu
authors.forename: H T
authors.initials: HT
authors.lastname: Liu
authors.forename: L J
authors.initials: LJ
authors.lastname: Zheng
authors.forename: Z
authors.initials: Z
authors.lastname: Qin
authors.forename: H X
authors.initials: HX
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs; D009361:Neoplasm Invasiveness; D014162:Transfection
chemical_list: D035683:MicroRNAs
keywords: Breast neoplasms; Cell proliferation; MicroRNAs; Neoplasm invasiveness