title: Modification of fatty acid selectivity of Candida antarctica lipase A by error-prone PCR.
abstract: OBJECTIVE
  To generate Candida antarctica lipase A (CAL-A) mutants with modified fatty acid selectivities and improved lipolytic activities using error-prone PCR (epPCR).
  
  
  RESULTS
  A Candida antarctica lipase A mutant was obtained in three rounds of epPCR. This mutant showed a 14 times higher ability to hydrolyze triacylglycerols containing conjugated linoleic acids, and was 12 and 14 times more selective towards cis-9, trans-11 and trans-10, cis-12 isomers respectively, compared to native lipase. Lipolytic activities towards fatty acid esters were markedly improved, in particular towards butyric, lauric, stearic and palmitic esters.
  
  
  CONCLUSIONS
  Directed molecular evolution is an efficient method to generate lipases with desirable selectivity towards CLA isomers and improved lipolytic activities towards esters of fatty acids.
authors.affiliation: Department of Food Biotechnology, University of Warmia and Mazury in Olsztyn, Heweliusz 1 Str., 10-718, Olsztyn, Poland. dagmara.glod@uwm.edu.pl.|Department of Pathophysiology, University of Warmia and Mazury in Olsztyn, al. Warszawska 30, 10-082, Olsztyn, Poland. dagmara.glod@uwm.edu.pl.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Głód
mesh_terms: D002175:Candida / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D019020:Directed Molecular Evolution / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D015202:Protein Engineering / Q000379:methods; D014280:Triglycerides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D044243:Linoleic Acids, Conjugated; D014280:Triglycerides; D008049:Lipase
keywords: Conjugated linoleic acid; Directed molecular evolution; Error-prone PCR; Lipase; Protein engineering; Selectivity