title: FTO Promotes Adipogenesis through Inhibition of the Wnt/β-catenin Signaling Pathway in Porcine Intramuscular Preadipocytes.
abstract: Numerous studies have demonstrated that FTO plays an important role in adipogenesis. Herein, we designed a small interfering RNA targeting FTO to knock down its endogenous expression and investigated its effects on the proliferation and differentiation of porcine intramuscular preadipocytes. Its possible mechanism was also investigated. We showed that FTO silencing significantly decreased the level of phospho-Histone H3 protein and inhibited the proliferation of porcine intramuscular preadipocytes. In addition, the expressions of peroxisome proliferators-activated receptor γ (PPARγ) and CAAT/enhancer binding protein (C/EBPα) were down-regulated, but the expression of β-catenin was up-regulated, by FTO silencing. Of specific interest here was that LiCl, a Wnt/β-catenin signaling specific activator, attenuated the FTO-induced upregulation of PPARγ and downregulation of β-catenin. Collectively, our data demonstrated that FTO silence decreased the proliferation and differentiation of porcine intramuscular preadipocytes, and FTO affects the porcine intramuscular preadipocytes differentiation might be via Wnt/β-catenin signaling pathway.
authors.affiliation: a Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education , Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , P. R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: a Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education , Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , P. R. China.
authors.forename: Yanliu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: a Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education , Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.affiliation: a Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education , Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , P. R. China.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.affiliation: a Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education , Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , P. R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.affiliation: a Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education , Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , P. R. China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D034741:RNA, Small Interfering / Q000235:genetics; D013552:Swine; D060449:Wnt Signaling Pathway / Q000235:genetics / Q000502:physiology*
chemical_list: D034741:RNA, Small Interfering; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO
keywords: Differentiation; FTO; Wnt/β-catenin signaling; porcine intramuscular preadipocytes