title: Metabolic crosstalk between membrane and storage lipids facilitates heat stress management in Schizosaccharomyces pombe.
abstract: Cell membranes actively participate in stress sensing and signalling. Here we present the first in-depth lipidomic analysis to characterize alterations in the fission yeast Schizosaccharomyces pombe in response to mild heat stress (HS). The lipidome was assessed by a simple one-step methanolic extraction. Genetic manipulations that altered triglyceride (TG) content in the absence or presence of HS gave rise to distinct lipidomic fingerprints for S. pombe. Cells unable to produce TG demonstrated long-lasting growth arrest and enhanced signalling lipid generation. Our results reveal that metabolic crosstalk between membrane and storage lipids facilitates homeostatic maintenance of the membrane physical/chemical state that resists negative effects on cell growth and viability in response to HS. We propose a novel stress adaptation mechanism in which heat-induced TG synthesis contributes to membrane rigidization by accommodating unsaturated fatty acids of structural lipids, enabling their replacement by newly synthesized saturated fatty acids.
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Péter
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Glatz
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Gudmann
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Imre
authors.initials: I
authors.lastname: Gombos
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Török
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Ibolya
authors.initials: I
authors.lastname: Horváth
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Vígh
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Gábor
authors.identifier: http://orcid.org/0000-0002-4565-6850
authors.initials: G
authors.lastname: Balogh
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D018869:Heat-Shock Response / Q000502:physiology*; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D008563:Membrane Lipids / Q000378:metabolism; D012568:Schizosaccharomyces / Q000254:growth & development / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D015398:Signal Transduction; D014280:Triglycerides / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D008055:Lipids; D008563:Membrane Lipids; D029702:Schizosaccharomyces pombe Proteins; D014280:Triglycerides