title: Stage-specific functions of Semaphorin7A during adult hippocampal neurogenesis rely on distinct receptors.
abstract: The guidance protein Semaphorin7A (Sema7A) is required for the proper development of the immune and nervous systems. Despite strong expression in the mature brain, the role of Sema7A in the adult remains poorly defined. Here we show that Sema7A utilizes different cell surface receptors to control the proliferation and differentiation of neural progenitors in the adult hippocampal dentate gyrus (DG), one of the select regions of the mature brain where neurogenesis occurs. PlexinC1 is selectively expressed in early neural progenitors in the adult mouse DG and mediates the inhibitory effects of Sema7A on progenitor proliferation. Subsequently, during differentiation of adult-born DG granule cells, Sema7A promotes dendrite growth, complexity and spine development through β1-subunit-containing integrin receptors. Our data identify Sema7A as a key regulator of adult hippocampal neurogenesis, providing an example of how differential receptor usage spatiotemporally controls and diversifies the effects of guidance cues in the adult brain.
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.
authors.forename: Bart C
authors.initials: BC
authors.lastname: Jongbloets
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Lemstra
authors.affiliation: Dipartimento di Scienze della Vita e Biologia dei Sistemi and Neuroscience Institute Cavalieri Ottolenghi, University of Torino, 10100 Torino, Italy.
authors.forename: Roberta
authors.identifier: 0000-0002-2504-5585
authors.initials: R
authors.lastname: Schellino
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.
authors.forename: Mark H
authors.initials: MH
authors.lastname: Broekhoven
authors.affiliation: Centre for Animal Sciences, School of Basic and Applied Sciences, Central University Punjab, City Campus, Mansa Road, Bathinda 151001, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Parkash
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.
authors.forename: Anita J C G M
authors.initials: AJ
authors.lastname: Hellemons
authors.affiliation: Vollum Institute, Oregon Health &Science University, Portland, Oregon 97239, USA.
authors.forename: Tianyi
authors.identifier: 0000-0002-3532-8319
authors.initials: T
authors.lastname: Mao
authors.affiliation: Inserm, Laboratory of Development and Plasticity of the Neuroendocrine Brain, Jean-Pierre Aubert Research Centre, U1172, 59045 Lille, France.|University of Lille, 59045 Lille, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Giacobini
authors.affiliation: Neuroplasticity and Behavior Unit, Laboratory of Neurosciences, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA.
authors.forename: Henriette
authors.initials: H
authors.lastname: van Praag
authors.affiliation: Dipartimento di Scienze della Vita e Biologia dei Sistemi and Neuroscience Institute Cavalieri Ottolenghi, University of Torino, 10100 Torino, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: De Marchis
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.
authors.forename: Geert M J
authors.initials: GM
authors.lastname: Ramakers
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.
authors.forename: R Jeroen
authors.initials: RJ
authors.lastname: Pasterkamp
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D019012:Integrin beta1 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D055495:Neurogenesis / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013238:Stereotaxic Techniques; D013702:Temporal Lobe / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D019012:Integrin beta1; D009419:Nerve Tissue Proteins; C492112:Plxna3 protein, mouse; D011956:Receptors, Cell Surface; C485571:Sema7a protein, mouse; D039961:Semaphorins