title: Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide.
abstract: Erwinia amylovora(E. amylovora) is the first bacterial plant pathogen described and demonstrated to cause fire blight, a devastating plant disease affecting a wide range of species including a wide variety ofRosaceae. In this study, we reported the lipopolysaccharide (LPS) core structure fromE. amylovorastrain CFBP1430, the first one for anE. amylovorahighly pathogenic strain. The chemical characterization was performed on the mutantswaaL(lacking only the O-antigen LPS with a complete LPS-core),wabHandwabG(outer-LPS core mutants). The LPSs were isolated from dry cells and analyzed by means of chemical and spectroscopic methods. In particular, they were subjected to a mild acid hydrolysis and/or a hydrazinolysis and investigated in detail by one and two dimensional Nuclear Magnetic Resonance (NMR) spectroscopy and ElectroSpray Ionization Fourier Transform-Ion Cyclotron Resonance (ESI FT-ICR) mass spectrometry.
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy. angela.casillo@unina.it.
authors.forename: Angela
authors.initials: A
authors.lastname: Casillo
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy. marcello.ziaco@unina.it.
authors.forename: Marcello
authors.initials: M
authors.lastname: Ziaco
authors.affiliation: Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, D-23845 Borstel, Germany. blindner@fz-borstel.de.
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.affiliation: Department of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain. smerino@ub.edu.
authors.forename: Susana
authors.initials: S
authors.lastname: Merino
authors.affiliation: Department of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain. elenademendoza@hotmail.com.
authors.forename: Elena
authors.initials: E
authors.lastname: Mendoza-Barberá
authors.affiliation: Department of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain. jtomas@ub.edu.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tomás
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy. corsaro@unina.it.
authors.forename: Maria Michela
authors.initials: MM
authors.lastname: Corsaro
mesh_terms: D000107:Acetylation; D044084:Erwinia amylovora / Q000737:chemistry* / Q000235:genetics; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008070:Lipopolysaccharides; D009844:Oligosaccharides
keywords: ESI FT-ICR; Erwinia amylovora; NMR; lipopolysaccharide; structural determination