title: Preparation of a nano- and micro-fibrous decellularized scaffold seeded with autologous mesenchymal stem cells for inguinal hernia repair.
abstract: Prosthetic meshes used for hernioplasty are usually complicated with chronic pain due to avascular fibrotic scar or mesh shrinkage. In this study, we developed a tissue-engineered mesh (TEM) by seeding autologous bone marrow-derived mesenchymal stem cells onto nanosized fibers decellularized aorta (DA). DA was achieved by decellularizing the aorta sample sequentially with physical, mechanical, biological enzymatic digestion, and chemical detergent processes. The tertiary structure of DA was constituted with micro-, submicro-, and nanosized fibers, and the original strength of fresh aorta was retained. Inguinal hernia rabbit models were treated with TEMs or acellular meshes (AMs). After implantation, TEM-treated rabbit models showed no hernia recurrence, whereas AM-treated animals displayed bulges in inguinal area. At harvest, TEMs were thicker, have less adhesion, and have stronger mechanical strength compared to AMs (P<0.05). Moreover, TEM showed better cell infiltration, tissue regeneration, and neovascularization (P<0.05). Therefore, these cell-seeded DAs with nanosized fibers have potential for use in inguinal hernioplasty.
authors.affiliation: Medical College, Xiamen University.
authors.forename: Yinlong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Medical College, Xiamen University.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Oncology and Vascular Intervention Radiology.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Medical College, Xiamen University.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Medical College, Xiamen University.
authors.forename: Jie
authors.initials: J
authors.lastname: Chai
authors.affiliation: Medical College, Xiamen University.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Medical College, Xiamen University.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Emergency.
authors.forename: Jinling
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Zhongshan Hospital, Xiamen University, Xiamen, People's Republic of China.
authors.forename: Yaozong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology and Vascular Intervention Radiology.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D004247:DNA / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006552:Hernia, Inguinal / Q000628:therapy*; D059685:Herniorrhaphy; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053758:Nanoparticles / Q000737:chemistry*; D019736:Prostheses and Implants; D011817:Rabbits; D012756:Sheep; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D003094:Collagen; D004247:DNA; D007770:L-Lactate Dehydrogenase
keywords: decellularized aorta; hernioplasty; inguinal hernia; nanobiomaterial; tissue engineering