title: A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets.
abstract: This study describes a sensitive (1.3 × 101genomic copies/μL) and specific TaqMan-based qRT-PCR assay able to detect and quantify SVA RNA in porcine biological samples. The technique represents an efficient tool for the virus diagnosis and assessment of SVA load in tissues of infected animals and for epidemiological studies.
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil.
authors.forename: Alais Maria
authors.initials: AM
authors.lastname: Dall Agnol
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil.
authors.forename: Rodrigo Alejandro Arellano
authors.initials: RAA
authors.lastname: Otonel
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil.
authors.forename: Raquel Arruda
authors.initials: RA
authors.lastname: Leme
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil. Electronic address: alfieri@uel.br.
authors.forename: Amauri Alcindo
authors.initials: AA
authors.lastname: Alfieri
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil.
authors.forename: Alice Fernandes
authors.initials: AF
authors.lastname: Alfieri
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000235:genetics / Q000821:virology; D010849:Picornaviridae / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D010850:Picornaviridae Infections / Q000175:diagnosis* / Q000235:genetics* / Q000662:veterinary / Q000821:virology; D013552:Swine / Q000235:genetics / Q000821:virology
keywords: Picornavirus infection; Quantitative PCR; Real-time RT-PCR; Seneca valley virus; Swine; Vesicular disease