title: Interaction Between Methicillin-Resistant Staphylococcus aureus (MRSA) and Acanthamoeba polyphaga.
abstract: The interactions that occur between bacteria and amoebae can give through mutual relations, where both organisms benefit from the association or parasitic in which one organism benefits at the expense of the other. When these organisms share the same environment, it can result in some changes in the growth of organisms, in adaptation patterns, in morphology, development or even in their ability to synthesize proteins and other substances. In this study, the interaction between Acanthamoeba polyphaga and Staphylococcus aureus (MRSA) was evaluated using a co-culture model at different incubation times. The results showed that 89% of amoebic cells remained viable after contact with the bacteria. The bacterial isolate was visualized inside the amoeba through confocal microscopy and fluorescence for up to 216 h of co-cultivation. The lysate of amoebic culture increased the growth of S. aureus (MRSA), and the effect of supernatant of culture inhibited bacterial growth over the incubation times, suggesting that A. polyphaga produced some metabolite, that inhibited the growth of bacteria. Moreover, the encystment of the A. polyphaga was increased by the bacteria presence. The results show that A. polyphaga and S. aureus interaction may have an important influence on survival of both, and specially indicate a possible effect on the metabolics characteristics each other.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Setor de Parasitologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, N° 500, Porto Alegre, RS, Brazil.
authors.forename: Thamires Klein
authors.initials: TK
authors.lastname: de Souza
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Setor de Parasitologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, N° 500, Porto Alegre, RS, Brazil.
authors.forename: Scheila Silva
authors.initials: SS
authors.lastname: Soares
authors.affiliation: Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul, Avenida Independência, N° 2293, Santa Cruz do Sul, RS, Brazil.
authors.forename: Lisianne Brittes
authors.initials: LB
authors.lastname: Benitez
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Setor de Parasitologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, N° 500, Porto Alegre, RS, Brazil. marilise.rott@ufrgs.br.
authors.forename: Marilise Brittes
authors.initials: MB
authors.lastname: Rott
mesh_terms: D000048:Acanthamoeba / Q000382:microbiology / Q000502:physiology*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D055624:Methicillin-Resistant Staphylococcus aureus / Q000502:physiology*; D050296:Microbial Viability; D000069289:Parasite Encystment; D013559:Symbiosis
chemical_list: D017077:Culture Media, Conditioned
keywords: Acanthamoeba polyphaga; Co-culture; Interaction; Staphylococcus aureus