title: SICLOPPS cyclic peptide libraries in drug discovery.
abstract: Cyclic peptide libraries have demonstrated significant potential when employed against challenging targets such as protein-protein interactions. While a variety of methods for library generation exist, genetically encoded libraries hold several advantages over their chemically synthesized counterparts; they are more readily accessible and allow straightforward hit deconvolution. One method for the intracellular generation of such libraries is split-intein circular ligation of peptides and proteins (SICLOPPS). Here we detail and discuss the deployment of SICLOPPS libraries for the identification of cyclic peptide inhibitors of a variety of targets.
authors.affiliation: Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom. Electronic address: ali1@soton.ac.uk.
authors.forename: Ali
authors.initials: A
authors.lastname: Tavassoli
mesh_terms: D000818:Animals; D055808:Drug Discovery / Q000379:methods*; D006801:Humans; D047668:Inteins; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D019151:Peptide Library; D010456:Peptides, Cyclic; D011506:Proteins