title: Automatic Quantification of the Number of Intracellular Compartments inArabidopsis thalianaRoot Cells.
abstract: In the era of quantitative biology, it is increasingly required to quantify confocal microscopy images. If possible, quantification should be performed in an automatic way, in order to avoid bias from the experimenter, to allow the quantification of a large number of samples, and to increase reproducibility between laboratories. In this protocol, we describe procedures for automatic counting of the number of intracellular compartments inArabidopsisroot cells, which can be used for example to study endocytosis or secretory trafficking pathways and to compare membrane organization between different genotypes or treatments. While developed forArabidopsisroots, this method can be used on other tissues, cell types and plant species.
authors.affiliation: Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, INRA, Lyon, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Bayle
authors.affiliation: Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, INRA, Lyon, France.
authors.forename: Matthieu Pierre
authors.initials: MP
authors.lastname: Platre
authors.affiliation: Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, INRA, Lyon, France.
authors.forename: Yvon
authors.initials: Y
authors.lastname: Jaillais
keywords: Arabidopsis; Compartment; Confocal analysis; Endocytosis; Image segmentation; Root; Spot detection