title: Quantitative phase imaging for cell culture quality control.
abstract: The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification of cell culture quality was explored. Label-free QPI of detached single cells in suspension was performed by Michelson interferometer-based self-interference DHM. Two pancreatic tumor cell lines were chosen as cellular model and analyzed for refractive index, volume, and dry mass under varying culture conditions. Firstly, adequate cell numbers for reliable statistics were identified. Then, to characterize the performance and reproducibility of the method, we compared results from independently repeated measurements and quantified the cellular response to osmolality changes of the cell culture medium. Finally, it was demonstrated that the evaluation of QPI images allows the extraction of absolute cell parameters which are related to cell layer confluence states. In summary, the results show that QPI enables label-free imaging cytometry, which provides novel complementary integral biophysical data sets for sophisticated quantification of cell culture quality with minimized sample preparation. © 2017 International Society for Advancement of Cytometry.
authors.affiliation: Biomedical Technology Center, University of Muenster, Mendelstr. 17, Muenster, D-48149, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Kastl
authors.affiliation: Biomedical Technology Center, University of Muenster, Mendelstr. 17, Muenster, D-48149, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Isbach
authors.affiliation: Department of Prosthetic Dentistry and Biomaterials, University of Muenster, Waldeyerstraße 30, Muenster, D-48149, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Dirksen
authors.affiliation: Biomedical Technology Center, University of Muenster, Mendelstr. 17, Muenster, D-48149, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schnekenburger
authors.affiliation: Biomedical Technology Center, University of Muenster, Mendelstr. 17, Muenster, D-48149, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Kemper
mesh_terms: D045744:Cell Line, Tumor; D006696:Holography / Q000379:methods*; D006801:Humans; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000473:pathology; D011786:Quality Control; D012815:Signal Processing, Computer-Assisted
keywords: cell culture quality; digital holographic microscopy; imaging cytometry; live cell imaging; quantitative phase imaging