title: Whole Cell Panning with Phage Display.
abstract: Phage display has emerged as one of the leading technologies for the selection of highly specific monoclonal antibodies, offering a number of advantages over traditional methods of antibody generation. While there are various possibilities to conduct phage display (e.g., solution panning, solid-phase panning), whole cell panning is an elegant way to present membrane embedded target antigens in their natural environment and conformation to antibody-bearing phages. Here, a whole cell panning procedure using a Fab-based antibody library including primary cell based screening for selectivity is described.
authors.affiliation: MorphoSys AG, Semmelweisstrasse 7, 82152, Planegg, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Stark
authors.affiliation: MorphoSys AG, Semmelweisstrasse 7, 82152, Planegg, Germany.
authors.forename: Sophie
authors.initials: S
authors.lastname: Venet
authors.affiliation: MorphoSys AG, Semmelweisstrasse 7, 82152, Planegg, Germany. annika.schmid@morphosys.com.
authors.forename: Annika
authors.initials: A
authors.lastname: Schmid
mesh_terms: D000941:Antigens / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics* / Q000276:immunology; D019151:Peptide Library
chemical_list: D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library
keywords: Antibody fragment; Antibody library; Phage display; Whole cell panning (WCP)