title: Isolation, characterization and evaluation of the probiotic potential of a novel Lactobacillus strain isolated from Feta-type cheese.
abstract: In the present study 45 lactic acid bacteria (LAB) strains were isolated from Feta-type cheese and were screened for probiotic potential in a series of established in vitro tests, including resistance to low pH, resistance to pepsin and pancreatin and tolerance to bile salts. The strain K5, which displayed properties similar to or even better than the reference strain Lactobacillus plantarum ATCC 14917, was chosen for further analysis. Firstly, multiplex PCR analysis indicated that the novel strain belongs to the paracasei species. Secondly, the susceptibility against common antibiotics was determined to ensure a safe exploitation of the potentially probiotic strain. Additionally, the performance of L. paracasei K5 as starter in the fermentation of pomegranate juice was studied to evaluate its technological properties. Finally, a novel multiplex PCR assay, based on random amplified polymorphic DNA (RAPD) analysis was developed for its efficient and accurate detection in food products.
authors.affiliation: Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, Greece. Electronic address: splessas@agro.duth.gr.
authors.forename: Stavros
authors.initials: S
authors.lastname: Plessas
authors.affiliation: Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, Greece.
authors.forename: Chrysanthi
authors.initials: C
authors.lastname: Nouska
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Karapetsas
authors.affiliation: Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, Greece.
authors.forename: Stavros
authors.initials: S
authors.lastname: Kazakos
authors.affiliation: Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Alexopoulos
authors.affiliation: Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Mantzourani
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.
authors.forename: Pelagia
authors.initials: P
authors.lastname: Chondrou
authors.affiliation: Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Fournomiti
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece. Electronic address: agalanis@mbg.duth.gr.
authors.forename: Alex
authors.initials: A
authors.lastname: Galanis
authors.affiliation: Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, Greece.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Bezirtzoglou
mesh_terms: D002611:Cheese / Q000032:analysis / Q000382:microbiology*; D005516:Food Microbiology; D007778:Lactobacillus / Q000737:chemistry*; D019936:Probiotics / Q000737:chemistry* / Q000302:isolation & purification
keywords: Lactobacillus paracasei; Multiplex PCR; Pomegranate beverage; Probiotic; RAPD