title: Hydrogen sulfide prevents postoperative adhesion in a rat uterine horn model.
abstract: OBJECTIVE
  Abdominal adhesions are primarily severe postoperative complications that can cause gynecological problems such as infertility and chronic pelvic pain. Inflammatory mediators are significantly related to adhesion formation, and hydrogen sulfide plays a significant anti-inflammatory role in multiple physiological processes. Therefore, the effect of NaHS, a hydrogen sulfide donor, on postoperative adhesion formation was examined in a rat uterine horn model.
  
  
  METHODS
  A rat uterine horn model was created to evaluate whether NaHS, a hydrogen sulfide donor, could decrease postoperative adhesion formation. Rats were randomly grouped and administrated with different doses of NaHS, where DL-propargylglycine and low-molecular-weight heparin acted as negative and positive controls, respectively. The extent and severity of adhesions were assessed on the 14thpostoperative day. Serum of rats was sampled for the determination of 27 cytokines using a chip.
  
  
  RESULTS
  The severity and total scores of adhesion in rats given 112μM/kg and 56μM/kg NaHS were significantly less compared with those of the control group (p<0.01). Scores for the extent of adhesion re-formation in the DL-propargylglycine and control groups did not differ (p>0.05). At least six cytokines were involved in the procedures for the prevention of adhesion formation, as they varied significantly among different groups.
  
  
  CONCLUSIONS
  Administration of NaHS could apparently reduce postoperative adhesion in the rat uterine horn model. This preventive effect may be associated with the variation of cytokine that is related to inflammatory.
authors.affiliation: Obstetrics & Gynecology Hospital, Fudan University, Shanghai, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Obstetrics & Gynecology Hospital, Fudan University, Shanghai, China; Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China; Institute of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Yi Zhun
authors.initials: YZ
authors.lastname: Zhu
authors.affiliation: Obstetrics & Gynecology Hospital, Fudan University, Shanghai, China; Institute of Biomedical Sciences, Fudan University, Shanghai, China; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Fudan University, Shanghai, China. Electronic address: XCJgroup@163.com.
authors.forename: Congjian
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D006862:Hydrogen Sulfide / Q000627:therapeutic use*; D023421:Models, Animal; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000517:prevention & control; D014599:Uterus / Q000601:surgery*
chemical_list: D000893:Anti-Inflammatory Agents; D006862:Hydrogen Sulfide
keywords: hydrogen sulfide; postoperative adhesion; rat uterine horn model