title: Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function:in vivoinsights from MCT1 haploinsufficient mice.
abstract: The purpose of this study was to investigate the effects of a partial suppression of monocarboxylate transporter (MCT)-1 on skeletal muscle pH, energetics, and function (MCT1+/-mice). Twenty-four MCT1+/-and 13 wild-type (WT) mice were subjected to a rest-exercise-recovery protocol, allowing assessment of muscle energetics (by magnetic resonance spectroscopy) and function. The study included analysis of enzyme activities and content of protein involved in pH regulation. Skeletal muscle of MCT1+/-mice had lower MCT1 (-61%;P< 0.05) and carbonic anhydrase (CA)-II (-54%;P< 0.05) contents. Although intramuscular pH was higher in MCT1+/-mice at rest (P< 0.001), the mice showed higher acidosis during the first minute of exercise (P< 0.01). Then, the pH time course was similar among groups until exercise completion. MCT1+/-mice had higher specific peak (P< 0.05) and maximum tetanic (P< 0.01) forces and lower fatigability (P< 0.001) when compared to WT mice. We conclude that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1+/-mice remain unexplained.-Chatel, B., Bendahan, D., Hourdé, C., Pellerin, L., Lengacher, S., Magistretti, P., Fur, Y. L., Vilmen, C., Bernard, M., Messonnier, L. A. Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function:in vivoinsights from MCT1 haploinsufficient mice.
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France; benjamin.chatel@live.fr.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Chatel
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France.
authors.forename: David
authors.initials: D
authors.lastname: Bendahan
authors.affiliation: Laboratoire Interuniversitaire de Biologie de la Motricité, Université Savoie Mont Blanc, Chambéry, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hourdé
authors.affiliation: Department of Physiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Luc
authors.initials: L
authors.lastname: Pellerin
authors.affiliation: Department of Physiology, University of Lausanne, Lausanne, Switzerland.|Laboratory of Neuroenergetic and Cellular Dynamics, Brain and Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lengacher
authors.affiliation: Laboratory of Neuroenergetic and Cellular Dynamics, Brain and Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.|Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.
authors.forename: Pierre
authors.initials: P
authors.lastname: Magistretti
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Fur
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Vilmen
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Bernard
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France.|Laboratoire Interuniversitaire de Biologie de la Motricité, Université Savoie Mont Blanc, Chambéry, France.
authors.forename: Laurent A
authors.initials: LA
authors.lastname: Messonnier
mesh_terms: D000818:Animals; D001835:Body Weight; D024402:Carbonic Anhydrase II / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D006239:Haplotypes; D006706:Homeostasis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D024402:Carbonic Anhydrase II; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1
keywords: carbonic anhydrases; magnetic resonance spectroscopy; monocarboxylate transporters