title: An Overview on the Enhancement of Enantioselectivity and Stability of Microbial Epoxide Hydrolases.
abstract: Epoxide hydrolases (EHs; 3.3.2.x) catalyze the enantioselective ring opening of racemic epoxides to the corresponding enantiopure vicinal diols and remaining equivalent unreacted epoxides. These epoxides and diols are used for the synthesis of chiral drug intermediates. With an upsurge in the methods for identification of novel microbial EHs, a lot of EHs have been discovered and utilized for kinetic resolution of racemic epoxides. However, there is still a constraint on the account of limited EHs being successfully applied on the preparative scale for industrial biotransformations. This limitation has to be overcome before application of identified functional EHs on large scale. Many strategies such as optimizing reaction media, immobilizing EHs and laboratory-scale directed evolution of EHs have been adopted for enhancing the industrial potential of EHs. In this review, these approaches have been highlighted which can serve as a pathway for the enrichment of already identified EHs for their application on an industrial scale in future studies.
authors.affiliation: Department of Biochemistry, Panjab University, Sector 25, BMS Block II, Chandigarh, 160014, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Saini
authors.affiliation: Department of Biochemistry, Panjab University, Sector 25, BMS Block II, Chandigarh, 160014, India. diptsare@pu.ac.in.
authors.forename: Dipti
authors.initials: D
authors.lastname: Sareen
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D055162:Biocatalysis; D004795:Enzyme Stability; D004851:Epoxide Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D015202:Protein Engineering; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D004852:Epoxy Compounds; D004851:Epoxide Hydrolases
keywords: Deep eutectic solvents; Epoxide hydrolase; Immobilization and directed evolution; Ionic liquids; Organic solvents