title: The novel high molecular weight Dermatophagoides farinae protein Zen-1 is a major allergen in North American and European mite allergic dogs with atopic dermatitis.
abstract: BACKGROUND
  Atopic dogs with hypersensitivity to Dermatophagoides farinae (Df) have IgE recognizing high molecular weight (MW) allergens more often than the low MW Der f 1 and 2. A new high MW Df allergen, Zen-1, has been identified recently.
  
  
  OBJECTIVE
  To determine the IgE reactivity of American and European Df-hypersensitive dogs to Zen-1, Der f 1 and Der f 2.
  
  
  METHODS
  We tested sera from 33 Df-reactive dogs from the USA, 29 from Europe and 15 experimentally sensitized to Df, by ELISA against crude Df, Der f 1, Der f 2 and Zen-1. ELISA inhibition was performed with sera reactive to Zen-1. Intradermal testing (IDT) was also done with the same allergens in 25 other American atopic dogs.
  
  
  RESULTS
  Altogether, IgE seropositivity to Zen-1 was more prevalent (86%) than that to Der f 1 (17%) or Der f 2 (19%). The IgE reactivity to Zen-1 was correlated to that against crude Df; this allergen alone inhibited a high percentage (median: 50%; range: 22-84%) of the binding to the crude mite extract. The seropositivity to low MW allergens was highest in experimentally sensitized dogs. Serum IgE recognition of Der f 1 was low in dogs with AD; that to Der f 2 was significantly lower in American dogs (6%) than in European ones (28%). A high prevalence of positive immediate IDT reactions to Zen-1 confirmed the likely relevance of serological results.
  
  
  CONCLUSIONS
  This study establishes Zen-1 as a major allergen in atopic dogs sensitized to Df.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA.|Comparative Medicine Institute, NC State University, Raleigh, NC, 27606, USA.
authors.forename: Thierry
authors.identifier: http://orcid.org/0000-0003-1399-0034
authors.initials: T
authors.lastname: Olivry
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA.
authors.forename: Stanley M
authors.initials: SM
authors.lastname: Dunston
authors.affiliation: Clinic for Small Animal Internal Medicine, Dermatology Department, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH-8057, Zürich, Switzerland.
authors.forename: Claude
authors.initials: C
authors.lastname: Favrot
authors.affiliation: Clinique Vétérinaire Advetia, 5 Rue Dubrunfaut, F-75012, Paris, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Prélaud
authors.affiliation: Animal Life Science Laboratories, Nippon Zenyaku Kogyo Corporation (Zenoaq), 1-1 Tairanoue, Sasagawa, Asaka-machi, Koriyama, Fukushima, 963-0196, Japan.
authors.forename: Toshiroh
authors.initials: T
authors.lastname: Tsukui
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005060:Europe / Q000453:epidemiology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D019476:Insect Proteins / Q000276:immunology*; D007428:Intradermal Tests / Q000662:veterinary; D014481:United States / Q000453:epidemiology
chemical_list: D039741:Antigens, Dermatophagoides; D019476:Insect Proteins; D007073:Immunoglobulin E