title: A novel tool reflecting the role of oxidative stress in the cataracts: thiol/disulfide homeostasis.
abstract: We investigated serum and aqueous humor thiol/disulfide (T-D) homeostasis in patients with cataracts versus healthy controls. In total, 56 patients with cataracts and 49 healthy controls were enrolled in this case-control study. Serum total thiol (TT), native thiol (NT), and disulfide (DS) concentrations were determined using a novel automated measurement method. Additionally, DS/TT, DS/NT and NT/TT percentage ratios were compared between the groups. In comparison with the control group, serum NT levels and aqueous humor TT and NT levels were significantly lower (p < .05, p < .05 and p < .001, respectively), whereas serum and aqueous humor DS levels were significantly higher in cataract patients (p < .01 and p < .001). DS/TT and DS/NT ratios were significantly higher and the NT/TT ratio was lower in cataract patients in serum (p < .005) and aqueous humor samples (p < .001). In conclusion, serum T-D homeostasis may be useful as biochemical markers, indicating the role of oxidative stress in the development of cataracts. Further studies are needed to confirm the pathophysiological role of T-D homeostasis in cataractogenesis.
authors.affiliation: a Department of Ophthalmology , Bezmialem University School of Medicine , Istanbul , Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Elbay
authors.affiliation: b Department of Biochemistry , Bezmialem University School of Medicine , Istanbul , Turkey.
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Ozer
authors.affiliation: c Department of Ophthalmology , Nevsehir State Hospital , Nevsehir , Turkey.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Altinisik
authors.affiliation: d Department of Ophthalmology , Samsun Gazi State Hospital , Samsun , Turkey.
authors.forename: Arif Emre
authors.initials: AE
authors.lastname: Elbay
authors.affiliation: a Department of Ophthalmology , Bezmialem University School of Medicine , Istanbul , Turkey.
authors.forename: Taha
authors.initials: T
authors.lastname: Sezer
authors.affiliation: a Department of Ophthalmology , Bezmialem University School of Medicine , Istanbul , Turkey.
authors.forename: Havvanur
authors.initials: H
authors.lastname: Bayraktar
authors.affiliation: a Department of Ophthalmology , Bezmialem University School of Medicine , Istanbul , Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Ozdemir
mesh_terms: D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002386:Cataract / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D002387:Cataract Extraction; D004220:Disulfides / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D015415:Biomarkers; D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: Cataract; disulfide; markers of oxidative stress; native thiol; thiol/disulfide homeostasis; total thiol