title: Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant.
abstract: Bacillus natto is commonly used in industrial production of menaquinone-7, an important vitamin which plays a crucial role for blood clotting and may contribute to prevention of cardiovascular disease and osteoporosis. This study determined the optimal combination of key nutrients and established an effective use of surfactant in a coupling medium to enhance the yield of extracellular MK-7. MK-7 yield of 31.18 mg/L was achieved under optimal conditions containing 53.6 g/L glycerol, 100 g/L soy peptone, and 10 g/L K2HPO4. A maximal yield of 40.96 mg/L MK-7 and a secretion ratio of 61.1% were obtained when 20 g/L soybean oil was supplemented at the logarithmic phase. The non-ionic surfactant span 20 was the second most promising surfactant in improving product yield, whereas addition of 2 g/L betaine exerted a minimal effect on secretion ratio of MK-7 at 19.1%. The results collectively showed that the supplementation of surfactants was an effective strategy to regulate cytomembrane permeability. Graphical abstract ᅟ.
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
authors.forename: Xue-Chao
authors.initials: XC
authors.lastname: Hu
authors.affiliation: Bioengineering Institute, Department of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People's Republic of China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Liu
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Luo
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China. renlujing@njtech.edu.cn.
authors.forename: Lu-Jing
authors.initials: LJ
authors.lastname: Ren
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China. biotech@njtech.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D001709:Biotechnology; D005285:Fermentation / Q000187:drug effects; D006601:Hexoses / Q000494:pharmacology*; D013024:Soybean Oil / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D006601:Hexoses; D013501:Surface-Active Agents; D024482:Vitamin K 2; C014822:sorbitan monolaurate; D013024:Soybean Oil; C062629:menaquinone 7
keywords: Bacillus natto; Menaquinone-7; Optimization; Secretion ratio; Surfactant