title: Laser irradiated fluorescent perfluorocarbon microparticles in 2-D and 3-D breast cancer cell models.
abstract: Perfluorocarbon (PFC) droplets were studied as new generation ultrasound contrast agents via acoustic or optical droplet vaporization (ADV or ODV). Little is known about the ODV irradiated vaporization mechanisms of PFC-microparticle complexs and the stability of the new bubbles produced. In this study, fluorescent perfluorohexane (PFH) poly(lactic-co-glycolic acid) (PLGA) particles were used as a model to study the process of particle vaporization and bubble stability following excitation in two-dimensional (2-D) and three-dimensional (3-D) cell models. We observed localization of the fluorescent agent on the microparticle coating material initially and after vaporization under fluorescence microscopy. Furthermore, the stability and growth dynamics of the newly created bubbles were observed for 11 min following vaporization. The particles were co-cultured with 2-D cells to form 3-D spheroids and could be vaporized even when encapsulated within the spheroids via laser irradiation, which provides an effective basis for further work.
authors.affiliation: Department of Ultrasound Diagnosis, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Niu
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Second Affiliated Hospital, Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing, 400010, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Ultrasound Diagnosis, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Yihe
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Ultrasound Diagnosis, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Peng
mesh_terms: D000162:Acoustics; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism; D003287:Contrast Media / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005466:Fluorocarbons / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D007834:Lasers; D061986:MCF-7 Cells; D045423:Microbubbles; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D018874:Spheroids, Cellular / Q000378:metabolism / Q000473:pathology*; D016217:Ultrasonography, Mammary / Q000379:methods*; D014835:Volatilization
chemical_list: C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines; D003287:Contrast Media; D005456:Fluorescent Dyes; D005466:Fluorocarbons; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C078626:perflexane