title: Monitoring of Crosstalk Between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots.
abstract: Over the last few years, it became more and more evident that plant hormone action is to great parts determined through their sophisticated crosstalk, rather than by their isolated activities. Thus, the parallel analysis of interconnected phytohormones in only very small amounts of tissue developed to an important issue in the field of plant sciences. In the following, a highly sensitive and accurate method is described for the quantitative analysis of the plant hormones jasmonic acid and indole-3-acetic acid in the model plant Arabidopsis thaliana. The described methodology is, however, not limited to the analysis of Arabidopsis samples but can also be applied to other plant species. The presented method is optimized for the working up of as little as 20-50 mg of plant tissue. Thus, it is well suited for the analysis of plant hormone contents in plant tissue of only little biomass, such as roots. The presented protocol facilitates the implementation of the method into other laboratories that have access to appropriate laboratory equipment and comparable state-of-the-art gas chromatography-mass spectrometry (GC-MS) technology.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA), Campus de Montegancedo, Autovía M-40, km 38, 28223, Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Víctor Carrasco
authors.initials: VC
authors.lastname: Loba
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA), Campus de Montegancedo, Autovía M-40, km 38, 28223, Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Marta-Marina Pérez
authors.initials: MP
authors.lastname: Alonso
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA), Campus de Montegancedo, Autovía M-40, km 38, 28223, Pozuelo de Alarcón, Madrid, Spain. stephan.pollmann@upm.es.
authors.forename: Stephan
authors.initials: S
authors.lastname: Pollmann
mesh_terms: D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007210:Indoleacetic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007553:Isotope Labeling; D054883:Oxylipins / Q000737:chemistry / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D015398:Signal Transduction; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013312:Stress, Physiological
chemical_list: D003517:Cyclopentanes; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid
keywords: Auxin; Crosstalk; Derivatization; Electron impact tandem mass spectrometry (EI-GC-MS/MS); Jasmonic acid; Plant hormone analysis; Solid-phase extraction; Stable isotopes