title: Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine.
abstract: A silver nanoparticle (AgNP)-based fluorescence-quenching lateral flow immunoassay with competitive format (cLFIA) was developed for sensitive detection of ochratoxin A (OTA) in grape juice and wine samples in the present study. The Ru(phen) 3 2 + -doped silica nanoparticles (RuNPs) were sprayed on the test and control line zones as background fluorescence signals. The AgNPs were designed as the fluorescence quenchers of RuNPs because they can block the exciting light transferring to the RuNP molecules. The proposed method exhibited high sensitivity for OTA detection, with a detection limit of 0.06 µg/L under optimized conditions. The method also exhibited a good linear range for OTA quantitative analysis from 0.08 µg/L to 5.0 µg/L. The reliability of the fluorescence-quenching cLFIA method was evaluated through analysis of the OTA-spiked red grape wine and juice samples. The average recoveries ranged from 88.0% to 110.0% in red grape wine and from 92.0% to 110.0% in grape juice. Meanwhile, less than a 10% coefficient variation indicated an acceptable precision of the cLFIA method. In summary, the new AgNP-based fluorescence-quenching cLFIA is a simple, rapid, sensitive, and accurate method for quantitative detection of OTA in grape juice and wine or other foodstuffs.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. riverhu@163.com.|Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China. riverhu@163.com.
authors.forename: Hu
authors.initials: H
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. lixiangmin73@163.com.|Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China. lixiangmin73@163.com.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. yingxiong201468@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. pk415917ibs@126.com.
authors.forename: Ke
authors.initials: K
authors.lastname: Pei
authors.affiliation: Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China. lijuannie2016@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. yhxiongchen@163.com.|Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China. yhxiongchen@163.com.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D007118:Immunoassay; D053768:Metal Nanoparticles; D009793:Ochratoxins / Q000032:analysis* / Q000276:immunology; D012428:Ruthenium; D012834:Silver; D027843:Vitis; D014920:Wine / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D009793:Ochratoxins; C025589:ochratoxin A; D012834:Silver; D012428:Ruthenium
keywords: fluorescence quenching; lateral flow immunoassay; ochratoxin A; quantitative detection; silver nanoparticles