title: Lipoic Acid as a Possible Pharmacological Source of Hydrogen Sulfide/Sulfane Sulfur.
abstract: The aim of the present study was to verify whether lipoic acid (LA) itself is a source of H₂S and sulfane sulfur. It was investigated in vitro non-enzymatically and enzymatically (in the presence of rat tissue homogenate). The results indicate that both H₂S and sulfane sulfur are formed from LA non-enzymatically in the presence of environmental light. These results suggest that H₂S is the first product of non-enzymatic light-dependent decomposition of LA that is, probably, next oxidized to sulfane sulfur-containing compound(s). The study performed in the presence of rat liver and kidney homogenate revealed an increase of H₂S level in samples containing LA and its reduced form dihydrolipoic acid (DHLA). It was accompanied by a decrease in sulfane sulfur level. It seems that, in these conditions, DHLA acts as a reducing agent that releases H₂S from an endogenous pool of sulfane sulfur compounds present in tissues. Simultaneously, it means that exogenous LA cannot be a direct donor of H₂S/sulfane sulfur in animal tissues. The present study is an initial approach to the question whether LA itself is a donor of H₂S/sulfane sulfur.
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Collegium Medicum, 7 Kopernika Street, 31-034 Kraków, Poland. mbbilska@cyf-kr.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Bilska-Wilkosz
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Collegium Medicum, 7 Kopernika Street, 31-034 Kraków, Poland. miciek@cm-uj.krakow.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Iciek
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Collegium Medicum, 7 Kopernika Street, 31-034 Kraków, Poland. dkowalczyk@cm-uj.krakow.pl.
authors.forename: Danuta
authors.initials: D
authors.lastname: Kowalczyk-Pachel
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Collegium Medicum, 7 Kopernika Street, 31-034 Kraków, Poland. mbgorny@cyf-kr.edu.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Górny
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Collegium Medicum, 7 Kopernika Street, 31-034 Kraków, Poland. marsokol@wp.pl.
authors.forename: Maria
authors.initials: M
authors.lastname: Sokołowska-Jeżewicz
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Collegium Medicum, 7 Kopernika Street, 31-034 Kraków, Poland. mbwlodek@cyf-kr.edu.pl.
authors.forename: Lidia
authors.initials: L
authors.lastname: Włodek
mesh_terms: D000818:Animals; D002384:Catalysis; D004492:Edetic Acid / Q000737:chemistry; D006862:Hydrogen Sulfide / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D013457:Sulfur Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000737:chemistry*; D013997:Time Factors
chemical_list: D013457:Sulfur Compounds; D008063:Thioctic Acid; C015144:dihydrolipoic acid; D004492:Edetic Acid; D006862:Hydrogen Sulfide
keywords: dihydrolipoic acid; hydrogen sulfide; lipoic acid; sulfane sulfur