title: Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres.
abstract: We have developed a 3D coaxial flow-focusing nozzle device for the mass production of monodispersed collagen microspheres and chemically crosslinked them using EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide) and N-hydroxysuccinimide (NHS). The size of the microspheres was varied between 200 μm and 600 μm by adjusting the ratio of the flow rates of the dispersed and continuous phases. MDA231-GFP cells were attached to the surface of these particles and their viability was investigated. Because they are comprised of a natural biomaterial, these collagen microspheres will have numerous applications, including bone regeneration scaffolds for tissue engineering and analyses of cancer cell interactions in a 3D environment.
authors.forename: Sravani
authors.initials: S
authors.lastname: Jaligama
authors.lastname: Po-Jung Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Kameoka
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D013388:Succinimides / Q000737:chemistry; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D013388:Succinimides; D003094:Collagen; C001426:N-hydroxysuccinimide