title: Changes in the mitochondrial protein profile due to ROS eruption during ageing of elm (Ulmus pumila L.) seeds.
abstract: Reactive oxygen species (ROS)-related mitochondrial dysfunction is considered to play a vital role in seed deterioration. However, the detailed mechanisms remain largely unknown. To address this, a comparison of mitochondrial proteomes was performed, and we identified several proteins that changed in abundance with accompanying ROS eruption and mitochondrial aggregation and diffusion. These are involved in mitochondrial metabolisms, stress resistance, maintenance of structure and intracellular transport during seed aging. Reduction of ROS content by the mitochondrial-specific scavenger MitoTEMPO suppressed these changes, whereas pre-treatment of seeds with methyl viologen (MV) had the opposite effect. Furthermore, voltage-dependent anion channels (VDAC) were found to increase both in abundance and carbonylation level, accompanied by increased cytochrome c (cyt c) release from mitochondria to cytosol, indicating the profound effect of ROS and VDAC on mitochondria-dependent cell death. Carbonylation detection revealed the specific target proteins of oxidative modification in mitochondria during ageing. Notably, membrane proteins accounted for a large proportion of these targets. An in vitro assay demonstrated that the oxidative modification was concomitant with a change of VDAC function and a loss of activity in malate dehydrogenase. Our data suggested that ROS eruption induced alteration and modification of specific mitochondrial proteins that may be involved in the process of mitochondrial deterioration, which eventually led to loss of seed viability.
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35, Tsinghua East Road, Beijing, PR China. Electronic address: luckycatliying@foxmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35, Tsinghua East Road, Beijing, PR China. Electronic address: wangyu_bio@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35, Tsinghua East Road, Beijing, PR China. Electronic address: xuehuasnowsnow@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Xue
authors.affiliation: Royal Botanic Gardens, Kew, Wellcome Trust Millennium Building, Wakehurst Place, Ardingly, RH17 6TN, UK. Electronic address: h.pritchard@kew.org.
authors.forename: Hugh W
authors.initials: HW
authors.lastname: Pritchard
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35, Tsinghua East Road, Beijing, PR China. Electronic address: wxf801@sina.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D016923:Cell Death; D019069:Cell Respiration; D045304:Cytochromes c / Q000378:metabolism; D018525:Germination; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D050050:Protein Carbonylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D034403:Ulmus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D050995:Voltage-Dependent Anion Channel 1 / Q000378:metabolism
chemical_list: C555916:MitoTEMPO; D024101:Mitochondrial Proteins; D009943:Organophosphorus Compounds; D010880:Piperidines; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D045304:Cytochromes c; D050995:Voltage-Dependent Anion Channel 1; D010269:Paraquat
keywords: Ageing; Elm (Ulmus pumila L.) seed; Mitochondria; Protein carbonylation; ROS; VDAC