title: Probing deep into the binding mechanisms of folic acid with α-amylase, pepsin and trypsin: An experimental and computational study.
abstract: The inhibitions of folic acid (FA) towards three digestive enzymes, including α-amylase, pepsin and trypsin, were examined. The results showed that FA was able to reduce the enzymatic activity of α-amylase, pepsin, and trypsin by the formation of FA-enzyme complexes. The fluorescence spectral data indicated that the binding of FA with α-amylase, pepsin and trypsin resulted in strong fluorescence quenching of Tyr and Trp residues by hydrophobic interactions, hydrogen bonding and electrostatic interactions. To identify the precise binding sites of FA on α-amylase, pepsin and trypsin, the molecular modeling studies were also performed in this work. These investigations may constitute meaningful work for further advances in the mechanisms behind the interactions between FA and digestive enzymes.
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Shi
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: wyqing76@126.com.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: hxzhm@126.com.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address: feishenghao@163.com.
authors.forename: Zhenghao
authors.initials: Z
authors.lastname: Fei
mesh_terms: D005492:Folic Acid / Q000737:chemistry*; D008958:Models, Molecular; D010434:Pepsin A / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D005492:Folic Acid; D000516:alpha-Amylases; D014357:Trypsin; D010434:Pepsin A
keywords: Activity; Binding mechanism; Digestive enzyme; Folic acid; Molecular modeling