title: Understanding the cochlear implant environment by mapping perilymph proteomes from different species.
abstract: Cochlear implants operate within a bony channel of the cochlea, bathed in a fluid known as the perilymph. The perilymph is a complex fluid containing ions and proteins, which are known to actively interact with metallic electrodes. To improve our understanding of how cochlear implant performance varies in preclinical in vivo studies in comparison to human trials and patient outcomes, the protein composition (or perilymph proteome) is needed. Samples of perilymph were gathered from feline and guinea pig subjects and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) to produce proteomes and compare against the recently published human proteome. Over 64% of the proteins in the guinea pig proteome were found to be common to the human proteome. The proportions of apolipoproteins, enzymes and immunoglobulins showed little variation between the two proteomes, with other classes showing similarity. This establishes a good basis for comparison of results. The results for the feline profile showed less similarity with the human proteome and would not provide a quality comparison. This work highlights the suitability of the guinea pig to model the biological environment of the human cochlear and the need to carefully select models of the biological environment of a cochlear implant to more adequately translate in vitro and in vivo studies to the clinic.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Palmer
authors.forename: Megan S
authors.initials: MS
authors.lastname: Lord
authors.forename: Jeremy L
authors.initials: JL
authors.lastname: Pinyon
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Wise
authors.forename: Nigel H
authors.initials: NH
authors.lastname: Lovell
authors.forename: Paul M
authors.initials: PM
authors.lastname: Carter
authors.forename: Ya Lang
authors.initials: YL
authors.lastname: Enke
authors.forename: Gary D
authors.initials: GD
authors.lastname: Housley
authors.forename: Rylie A
authors.initials: RA
authors.lastname: Green
mesh_terms: D000818:Animals; D002415:Cats; D019929:Cochlear Implantation; D003054:Cochlear Implants; D006168:Guinea Pigs; D006801:Humans; D010498:Perilymph / Q000378:metabolism*; D020543:Proteome / Q000145:classification / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D020543:Proteome