title: Evaluation ofBurkholderia cepaciaComplex Bacteria Pathogenicity UsingCaenorhabditis elegans.
abstract: This protocol describes two biological assays to evaluate pathogenicity ofBurkholderia cepaciacomplex (Bcc) strains against the nematodeCaenorhabditis elegans.Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Naples, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Tedesco
authors.affiliation: Institute of Bioscience and Bioresources, National Research Council, Naples, Italy.
authors.forename: Elia
authors.initials: E
authors.lastname: Di Schiavi
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Naples, Italy.
authors.forename: Fortunato Palma
authors.initials: FP
authors.lastname: Esposito
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Naples, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: de Pascale