title: Immunohistochemical analysis of estrogen receptors in prostate and clinical correlation in men with benign prostatic hyperplasia.
abstract: Estrogens act through interaction with 2 receptor subtypes, ER alpha (ERα) and ER beta (ERβ), in human prostate. The aim of the present study was to semiquantitatively assess the differential expression of ER subtypes in human benign prostatic hyperplasia (BPH) by use of immunocytochemistry (IHC) methods and to explore their relationship with various measures of BPH.
  
  
  
  A total of 45 patients with BPH undergoing transurethral resection of the prostate and 22 patients with bladder cancer with normal prostate undergoing surveillance cystoscopy were studied as cases and controls, respectively. Quantitative immunolabeling of ER subtypes was scored by use of a semiquantitative scale. Also, correlations were assessed between ER levels in prostate and various measures of BPH.
  
  
  
  Overall, we found strong immunostaining for ERα in stroma and for ERβ in epithelium, respectively. The IHC score for ERα differed significantly between BPH patients and controls in both stroma (p≤0.001) and epithelium (p=0.008), respectively. The ERβ IHC score was also significantly higher in the epithelium of BPH patients (p=0.01). Also, we found a significant correlation between prostatic ER levels and various clinical measures of BPH.
  
  
  
  ERs may play an important role in the pathogenesis of BPH.
authors.affiliation: Department of Urology and Renal Transplantation, SMS Medical College & Attached Hospitals, Jaipur, India.
authors.forename: Goto
authors.initials: G
authors.lastname: Gangkak
authors.affiliation: Department of Urology and Renal Transplantation, SMS Medical College & Attached Hospitals, Jaipur, India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Bhattar
authors.affiliation: Department of Pathology, SMS Medical College & Attached Hospitals, Jaipur, India.
authors.forename: Alka
authors.initials: A
authors.lastname: Mittal
authors.affiliation: Department of Urology and Renal Transplantation, SMS Medical College & Attached Hospitals, Jaipur, India.
authors.forename: Sher Singh
authors.initials: SS
authors.lastname: Yadav
authors.affiliation: Department of Urology and Renal Transplantation, SMS Medical College & Attached Hospitals, Jaipur, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Tomar
authors.affiliation: Department of Pathology, SMS Medical College & Attached Hospitals, Jaipur, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Department of Pathology, SMS Medical College & Attached Hospitals, Jaipur, India.
authors.forename: Jayanti
authors.initials: J
authors.lastname: Mehta
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D004958:Estradiol / Q000097:blood; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D011960:Receptors, Estrogen / Q000378:metabolism*; D013739:Testosterone / Q000097:blood; D020728:Transurethral Resection of Prostate / Q000379:methods
chemical_list: D015415:Biomarkers; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D019394:Ki-67 Antigen; D011960:Receptors, Estrogen; D013739:Testosterone; D004958:Estradiol
keywords: Antibodies; Estrogens; Immunohistochemistry; Prostate