title: Analytical Determination of Auxins and Cytokinins.
abstract: Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. Thanks to their different chemical structures, auxins, cytokinins and their metabolites are often determined separately, using specialized procedures of sample purification, extraction, and quantification. However, recent progress in the sensitivity of analytical methods of liquid chromatography coupled to mass spectrometry (LC-MS) allows parallel analysis of multiple compounds. Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures.
authors.affiliation: Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, CAS, Rozvojová 263, 165 02, Prague 6, Czech Republic. dobrev@ueb.cas.cz.
authors.forename: Petre I
authors.initials: PI
authors.lastname: Dobrev
authors.affiliation: Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, CAS, Rozvojová 263, 165 02, Prague 6, Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Hoyerová
authors.affiliation: Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, CAS, Rozvojová 263, 165 02, Prague 6, Czech Republic.|Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 128 44, Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Petrášek
mesh_terms: D002853:Chromatography, Liquid; D003583:Cytokinins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D007210:Indoleacetic Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D055432:Metabolomics / Q000379:methods; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D010937:Plant Growth Regulators / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010936:Plant Extracts; D010937:Plant Growth Regulators
keywords: Auxin; Cytokinins; Liquid chromatography; Mass spectrometry; Phytohormones