title: Global DNA methylation and oxidative stress biomarkers in workers exposed to metal oxide nanoparticles.
abstract: This is the first study to assess global methylation, oxidative DNA damage, and lipid peroxidation in workers with occupational exposure to metal oxide nanomaterials (NMs). Urinary and white blood cell (WBC) 8-hydroxydeoxyguanosine (8-OHdG), and exhaled breath condensate (EBC) 8-isoprostane were measured as oxidative stress biomarkers. WBC global methylation was measured as an epigenetic alteration. Exposure to TiO2, SiO2,and indium tin oxide (ITO) resulted in significantly higher oxidative biomarkers such as urinary 8-OHdG and EBC 8-isoprostane. However, significantly higher WBC 8-OHdG and lower global methylation were only observed in ITO handling workers. Significant positive correlations were noted between WBC and urinary 8-OHdG (Spearman correlation r=0.256, p=0.003). Furthermore, a significant negative correlation was found between WBC 8-OHdG and global methylation (r=-0.272, p=0.002). These results suggest that exposure to metal oxide NMs may lead to global methylation, DNA oxidative damage, and lipid peroxidation.
authors.affiliation: National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
authors.forename: Saou-Hsing
authors.initials: SH
authors.lastname: Liou
authors.affiliation: National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
authors.forename: Wei-Te
authors.initials: WT
authors.lastname: Wu
authors.affiliation: National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
authors.forename: Hui-Yi
authors.initials: HY
authors.lastname: Liao
authors.affiliation: Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan.
authors.forename: Chao-Yu
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan.
authors.forename: Cheng-Yen
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Jung
authors.affiliation: Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan. Electronic address: huilinglee3573@gmail.com.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Lee
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000652:urine; D001944:Breath Tests; D004249:DNA Damage; D019175:DNA Methylation; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D018384:Oxidative Stress; D010087:Oxides / Q000633:toxicity
chemical_list: D015415:Biomarkers; D010087:Oxides; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine
keywords: 8-Hydroxydeoxyguanosine; Biomarkers; LC–MS/MS; Metal oxide nanomaterials