title: A highly sensitive detection of chloramphenicol based on chemiluminescence immunoassays with the cheap functionalized Fe3O4@SiO2magnetic nanoparticles.
abstract: A strategy has been applied to chloramphenicol (CAP) detection with chemiluminescence immunoassays (CLIA) based on cheap functionalized Fe3O4@SiO2magnetic nanoparticles (Fe-MNPs). The strategy that bovine serum albumin (BSA) was immobilized on cheap functionalized Fe-MNPs and that the CAP molecules were then immobilized on BSA, avoided the long process of dialysis for preparation of the BSA-CAP conjugates. The samples were detected for both methods that utilized two different kinds of functionalized Fe-MNPs (amine-functionalized Fe3O4@SiO2and carboxylic acid-functionalized Fe3O4@SiO2). The sensitivities and limits of detection (LODs) of the two methods were obtained and compared based on inhibition curves. The 50% inhibition concentrations (IC50) values of the two methods were about 0.024 ng ml-1and 0.046 ng ml-1respectively and LODs were approximately 0.0002 ng ml-1and 0.001 ng ml-1respectively. These methods were much more sensitive than that of any traditional enzyme-linked immunosorbent assay (ELISA) previously reported. Therefore, such chemiluminescence methods could be easily adapted for small molecule detection in a variety of foods using Fe-MNPs.
authors.affiliation: Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors.forename: Wang
authors.identifier: http://orcid.org/0000-0002-6273-0530
authors.initials: W
authors.lastname: Linyu
authors.affiliation: Tongji University, Shanghai, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Manwen
authors.affiliation: High School Affiliated to Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chengzhi
authors.affiliation: Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002701:Chloramphenicol / Q000032:analysis*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine; D002701:Chloramphenicol; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide
keywords: chemiluminescent immunoassays; chloramphenicol; magnetic nanoparticles