title: Antioxidant enzyme profile and lipid peroxidation products in semen samples of testicular germ cell tumor patients submitted to orchiectomy.
abstract: To determine enzymatic antioxidant and lipid peroxidation levels in seminal plasma of patients orchiectomized for testicular tumors.
  
  
  
  The study included 52 patients: 26 control men and 26 orchiectomized patients for testicular tumor, of which 12 men had seminoma tumor and 14 men non-seminoma tumor. After semen analysis performed according to the WHO guidelines, an aliquot of semen was centrifuged and the seminal plasma was collected. Lipid peroxidation was performed by thiobarbituric acid reactive substances(TBARS) assay and antioxidant profile was assessed by analyzing catalase, glutathione per-oxidase (GPx) and superoxide anion (SOD) activities using colorimetric assays with a standard spectrophotometer. Data were tested for normality and compared using one-way ANOVA (p<0.05).
  
  
  
  Seminoma and non-seminoma groups presented lower sperm concentration and morphology when compared to control group (p=0.0001). Both study groups (seminoma and non-seminoma) presented higher TBARS levels when compared to control group (p=0.0000013). No differences were observed for SOD (p=0.646) and GPx (p=0.328). It was not possible to access the enzymatic activity of catalase in any group.
  
  
  
  Patients with testicular tumor present increased semen oxidative stress, but no differences were observed in antioxidant levels, even after orchiectomy. This indicates that most likely an increased generation of oxidative products takes place in these patients.
authors.affiliation: Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Camila
authors.initials: C
authors.lastname: Sposito
authors.affiliation: Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Camargo
authors.affiliation: Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Danielle Spinola
authors.initials: DS
authors.lastname: Tibaldi
authors.affiliation: Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Valéria
authors.initials: V
authors.lastname: Barradas
authors.affiliation: Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Agnaldo Pereira
authors.initials: AP
authors.lastname: Cedenho
authors.affiliation: Departamento de Reprodução Animal, Faculdade de Medicina Veterinária, Universidade de São Paulo, SP, Brasil.
authors.forename: Marcílio
authors.initials: M
authors.lastname: Nichi
authors.affiliation: Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Ricardo Pimenta
authors.initials: RP
authors.lastname: Bertolla
authors.affiliation: Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Deborah Montagnini
authors.initials: DM
authors.lastname: Spaine
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000378:metabolism; D003430:Cross-Sectional Studies; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009845:Oligospermia; D009919:Orchiectomy; D018384:Oxidative Stress / Q000502:physiology; D012661:Semen / Q000201:enzymology*; D055101:Semen Analysis; D018239:Seminoma / Q000378:metabolism*; D013076:Sperm Count; D013482:Superoxide Dismutase / Q000378:metabolism; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000601:surgery; D055815:Young Adult
chemical_list: D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidants ; Orchiectomy; Testicular Germ Cell Tumor