title: Volcanic Soils as Sources of Novel CO-OxidizingParaburkholderiaandBurkholderia:Paraburkholderia hiiakaesp. nov.,Paraburkholderia metrosiderisp. nov.,Paraburkholderia paradisisp. nov.,Paraburkholderia peleaesp. nov., andBurkholderia alpinasp. nov. a Member of theBurkholderia cepaciaComplex.
abstract: Previous studies showed that members of theBurkholderialeswere important in the succession of aerobic, molybdenum-dependent CO oxidizing-bacteria on volcanic soils. During these studies, four isolates were obtained from Kilauea Volcano (Hawai'i, USA); one strain was isolated from Pico de Orizaba (Mexico) during a separate study. Based on 16S rRNA gene sequence similarities, the Pico de Orizaba isolate and the isolates from Kilauea Volcano were provisionally assigned to the generaBurkholderiaandParaburkholderia, respectively. Each of the isolates possessed a form IcoxLgene that encoded the catalytic subunit of carbon monoxide dehydrogenase (CODH); none of the most closely related type strains possessedcoxLor oxidized CO. Genome sequences forParaburkholderiatype strains facilitated an analysis of 16S rRNA gene sequence similarities and average nucleotide identities (ANI). ANI did not exceed 95% (the recommended cutoff for species differentiation) for any of the pairwise comparisons among 27 reference strains related to the new isolates. However, since the highest 16S rRNA gene sequence similarity among this set of reference strains was 98.93%, DNA-DNA hybridizations (DDH) were performed for two isolates whose 16S rRNA gene sequence similarities with their nearest phylogenetic neighbors were 98.96 and 99.11%. In both cases DDH values were <16%. Based on multiple variables, four of the isolates represent novel species within theParaburkholderia:Paraburkholderia hiiakaesp. nov. (type strain I2T= DSM 28029T= LMG 27952T);Paraburkholderia paradisisp. nov. (type strain WAT= DSM 28027T= LMG 27949T);Paraburkholderia peleaesp. nov. (type strain PP52-1T= DSM 28028T= LMG 27950T); andParaburkholderia metrosiderisp. nov. (type strain DNBP6-1T= DSM 28030T= LMG 28140T). The remaining isolate represents the first CO-oxidizing member of theBurkholderia cepaciacomplex:Burkholderia alpinasp. nov. (type strain PO-04-17-38T= DSM 28031T= LMG 28138T).
authors.affiliation: Department of Biological Sciences, Louisiana State UniversityBaton Rouge, LA, USA; College of Health Sciences, Des Moines UniversityDes Moines, IA, USA.
authors.forename: Carolyn F
authors.initials: CF
authors.lastname: Weber
authors.affiliation: Department of Biological Sciences, Louisiana State University Baton Rouge, LA, USA.
authors.forename: Gary M
authors.initials: GM
authors.lastname: King
keywords: Burkholderia; Paraburkholderia; carbon monoxide; volcanic soils