title: Identification of lipopeptides produced by Bacillus subtilis Czk1 isolated from the aerial roots of rubber trees.
abstract: We obtained a strain of Bacillus subtilis, which we named Czk1, from the aerial roots of rubber trees. This bacterial isolate exhibits strong antagonistic activity against Ganoderma pseudoferreum, Phellinus noxius, Helicobasidium compactum, Rigidoporus lignosus, Sphaerostilbe repens, and Colletotrichum gloeosporioides. Our earlier research has shown that the antagonistic activity of a fermentation supernatant Czk1 isolate produces a complex mixture of lipopeptides. In this study, we used methanol to extract crude lipopeptides, purified them using a Sephadex G-25 column, cloned the lipopeptide genes, and analyzed purified fractions by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) to identify the lipopeptides from B. subtilis strain Czk1. The cloned lipopeptide genes included those that encode the enzymes lpa, ituD, sfp, and fenB. The crude lipopeptides were purified and found in five fractions. Further analysis revealed that five fractions of the purified composition contained members of the surfactin, iturin, fengycin, and bacillomycin families of antibiotics. This suggests that these lipopeptides from strain Czk1 have potential as plant disease biocontrol agents.
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.|College of Plant Science and Technology of Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: C P
authors.initials: CP
authors.lastname: He
authors.affiliation: College of Environment and Plant Protection, Hainan University, Haikou, Hainan, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Fan
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Wu
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Liang
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Environment and Plant Protection, Hainan University, Haikou, Hainan, China.
authors.forename: W
authors.initials: W
authors.lastname: Tang
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Zheng
authors.affiliation: College of Plant Science and Technology of Huazhong Agricultural University, Wuhan, Hubei, China xiaoyannong@mail.hzau.edu.cn.
authors.forename: Y N
authors.initials: YN
authors.lastname: Xiao
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China xiaoyannong@mail.hzau.edu.cn.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Liu
authors.affiliation: College of Environment and Plant Protection, Hainan University, Haikou, Hainan, China.
authors.forename: F C
authors.initials: FC
authors.lastname: Zheng
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D020231:Colletotrichum / Q000187:drug effects / Q000502:physiology; D028482:Hevea / Q000382:microbiology*; D055666:Lipopeptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000432:Methanol; D010456:Peptides, Cyclic / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C049972:fengycin; C052532:iturin D; C009365:surfactin peptide; C020977:bacillomycin S; D000432:Methanol