title: A metal ion regulated artificial metalloenzyme.
abstract: Regulation of enzyme activity is essential in living cells. The rapidly increasing number of designer enzymes with new-to-nature activities makes it necessary to develop novel strategies for controlling their catalytic activity. Here we present the development of a metal ion regulated artificial metalloenzyme created by combining two anchoring strategies, covalent and supramolecular, for introducing a regulatory and a catalytic site, respectively. This artificial metalloenzyme is activated in the presence of Fe2+ions, but only marginally in the presence of Zn2+.
authors.affiliation: Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands. j.g.roelfes@rug.nl.
authors.forename: Manuela
authors.initials: M
authors.lastname: Bersellini
authors.affiliation: Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands. j.g.roelfes@rug.nl.
authors.forename: Gerard
authors.identifier: http://orcid.org/0000-0002-0364-9564
authors.initials: G
authors.lastname: Roelfes
mesh_terms: D000494:Allosteric Regulation; D000495:Allosteric Site; D001426:Bacterial Proteins / Q000138:chemical synthesis / Q000737:chemistry; D020134:Catalytic Domain; D002413:Cations, Divalent / Q000737:chemistry; D004798:Enzymes / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007501:Iron / Q000737:chemistry*; D008667:Metalloproteins / Q000096:biosynthesis / Q000737:chemistry*; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000138:chemical synthesis / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002413:Cations, Divalent; D004798:Enzymes; D007501:Iron; D008667:Metalloproteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D015032:Zinc