title: Single-cell transcriptomics reveals specific RNA editing signatures in the human brain.
abstract: While RNA editing by A-to-I deamination is a requisite for neuronal function in humans, it is under-investigated in single cells. Here we fill this gap by analyzing RNA editing profiles of single cells from the brain cortex of living human subjects. We show that RNA editing levels per cell are bimodally distributed and distinguish between major brain cell types, thus providing new insights into neuronal dynamics.
authors.affiliation: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, 70126 Bari, Italy.|Institute of Biomembranes and Bioenergetics, National Research Council, 70126 Bari, Italy.
authors.forename: Ernesto
authors.identifier: 0000-0002-6549-0114
authors.initials: E
authors.lastname: Picardi
authors.affiliation: Department of Biosciences, University of Milan, 20133 Milan, Italy.
authors.forename: David S
authors.identifier: 0000-0002-6739-2657
authors.initials: DS
authors.lastname: Horner
authors.affiliation: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, 70126 Bari, Italy.|Institute of Biomembranes and Bioenergetics, National Research Council, 70126 Bari, Italy.
authors.forename: Graziano
authors.identifier: 0000-0003-3663-0859
authors.initials: G
authors.lastname: Pesole
mesh_terms: D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D016000:Cluster Analysis; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D009474:Neurons / Q000378:metabolism; D017393:RNA Editing; D059010:Single-Cell Analysis / Q000379:methods; D059467:Transcriptome
keywords: RNA editing; RNA-seq; single cell; transcriptome