title: Therapeutic effects of Schisandra chinensis on the hyperprolactinemia in rat.
abstract: Prolactin (PRL) is secreted from the pituitary gland in response to eating, mating, and ovulation. Increased serum concentration of PRL during pregnancy contributes to enlargement of the mammary glands of the breasts and prepares for production of milk. However, high PRL levels derived from prolactinoma and hyperprolactinemia induce physiological disorders such as infertility and early menopause. Natural compounds isolated from S. chinensis have been known to possess anti-oxidative, anti-inflammatory and anti-diabetic effects. In the present study, we examined the therapeutic effect of S. chinensis and its single compounds on hyperprolactinemia in the pituitary gland. In rat pituitary cells, PRL expression levels were examined using real-time PCR and western blot assay. Crude S. chinensis extract and its single compound, gomisin N, reduced mRNA and protein levels of PRL in GH3 cells. In addition, cell proliferation and PRL target gene expression in cells were modulated by S. chinensis. Similar to the in vitro experiments, crude S. chinensis extract and gomisin N reduced PRL levels in the pituitary and serum of immature female rats. These results show that S. chinensis and its single compound, gomisin N, are regulators of PRL production and may be candidates for treatment of hyperprolactinemia and prolactinoma.
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang‑si, Gyeongsangnam‑do, Republic of Korea.
authors.forename: So-Hye
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang-si, Gyeongsangnam‑do, Republic of Korea.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
authors.forename: Eui-Bae
authors.initials: EB
authors.lastname: Jeung
authors.affiliation: College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 200‑701, Republic of Korea.
authors.forename: Geun-Shik
authors.initials: GS
authors.lastname: Lee
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungnam National University, Daejeon, Gangwon, Republic of Korea.
authors.forename: Eui-Ju
authors.initials: EJ
authors.lastname: Hong
authors.affiliation: Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang-si, Gyeongsangnam‑do, Republic of Korea.
authors.forename: Young-Whan
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang‑si, Gyeongsangnam‑do, Republic of Korea.
authors.forename: Beum-Soo
authors.initials: BS
authors.lastname: An
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D034242:Cyclooctanes / Q000627:therapeutic use; D005260:Female; D005638:Fruit / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006966:Hyperprolactinemia / Q000188:drug therapy*; D017705:Lignans / Q000627:therapeutic use*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000188:drug therapy*; D010936:Plant Extracts / Q000627:therapeutic use*; D011083:Polycyclic Compounds / Q000627:therapeutic use*; D011388:Prolactin / Q000097:blood / Q000378:metabolism*; D015175:Prolactinoma / Q000188:drug therapy* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D032141:Schisandra / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D034242:Cyclooctanes; D017705:Lignans; D010936:Plant Extracts; D011083:Polycyclic Compounds; D012333:RNA, Messenger; C015499:schizandrin B; D011388:Prolactin