title: 1H,15N and13C resonance assignments and secondary structure of PulG, the major pseudopilin from Klebsiella oxytoca type 2 secretion system.
abstract: Bacteria use complex transporters to secrete functionally relevant proteins to the extracellular medium. The type 2 secretion system (T2SS) translocates folded proteins involved in bacterial nutrient acquisition, virulence and adaptation. The T2SS pseudopilus is a periplasmic filament, assembled by the polymerization of PulG subunits, the major pseudopilin. Pseudopilin proteins have a conserved N-terminal hydrophobic segment followed by a more variable C-terminal periplasmic and globular domain. To better understand the mechanism of assembly and function of the T2SS, we have been studying the structure and dynamics of PulG by NMR, as well as its interaction with other components of the secretion machinery. As a first step on this study, here we reported the chemical shift assignments of PulG C-terminal domain and its secondary structure prediction based on NMR data.
authors.affiliation: Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Dr Roux, Paris, France. aracelys.lopez-castilla@pasteur.fr.|CNRS UMR 3528, Paris, France. aracelys.lopez-castilla@pasteur.fr.
authors.forename: Aracelys
authors.initials: A
authors.lastname: López-Castilla
authors.affiliation: Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Dr Roux, Paris, France.|CNRS UMR 3528, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Vitorge
authors.affiliation: Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Dr Roux, Paris, France.|CNRS UMR 3528, Paris, France.
authors.forename: Léa
authors.initials: L
authors.lastname: Khoury
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Université Paris-Sud, Université Paris-Saclay, Avenue de la Terrasse, 91190, Gif-sur-Yvette, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Morellet
authors.affiliation: Laboratoire des Systèmes Macromoléculaires et Signalisation, Institut Pasteur, 25 rue du Dr Roux, Paris, France.
authors.forename: Olivera
authors.initials: O
authors.lastname: Francetic
authors.affiliation: Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Dr Roux, Paris, France. nadia.izadi@pasteur.fr.|CNRS UMR 3528, Paris, France. nadia.izadi@pasteur.fr.
authors.forename: Nadia
authors.identifier: http://orcid.org/0000-0002-3525-5949
authors.initials: N
authors.lastname: Izadi-Pruneyre
mesh_terms: D000595:Amino Acid Sequence; D041121:Klebsiella oxytoca; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D000069178:Type II Secretion Systems / Q000737:chemistry*
chemical_list: D000069178:Type II Secretion Systems
keywords: Chemical shift assignments; Klebsiella oxytoca; Major pseudopilin; PulG; Secondary structure; Type 2 secretion system