title: The Influence of Metal Ions on the Dynamics of Supported Phospholipid Langmuir Films.
abstract: The translational diffusion dynamics of the zwitterionic lipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a planar phosphorylated support surface containing metal ions (Mg2+, Ca2+, Ba2+, Ni2+, Zn2+, Cd2+, Zr4+) was investigated using X-ray photoelectron spectroscopy (XPS) and fluorescence recovery after photobleaching (FRAP). Fluorescence recovery curves yielded diffusion constants on the order of 2-5 μm2/s for the chromophore-tagged 12:0 NBD-Lyso-PC. Ionic interactions between the zwitterionic headgroup and metal ions were found to play a secondary role in mediating lipid fluidity. This work provides quantitative insight into the extent to which the fluidity of a supported lipid film is mediated by the ionic interactions between headgroup and surface versus that of the lipid-lipid tailgroup interactions.
authors.affiliation: Department of Chemistry, Michigan State University , 578 S. Shaw Lane, East Lansing, Michigan 48824, United States.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Baumler
authors.affiliation: Department of Chemistry, Michigan State University , 578 S. Shaw Lane, East Lansing, Michigan 48824, United States.
authors.forename: Gary J
authors.identifier: 0000-0002-1207-0810
authors.initials: GJ
authors.lastname: Blanchard
mesh_terms: D004058:Diffusion; D005453:Fluorescence; D008673:Metals, Alkaline Earth / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D013816:Thermodynamics
chemical_list: D008673:Metals, Alkaline Earth; D019216:Metals, Heavy; D010743:Phospholipids