title: "Activography": a novel, versatile and easily adaptable method for monitoring enzymatic activities in situ.
abstract: We developed activography to map enzymatic activities on tissue sections using activity-based probes. The assay was validated using a new protease-activity probe on skin biopsies to provide proof-of-concept. Activography is more selective and technically easier than the established in situ zymography, thus, adaptable in routine running clinico-chemical laboratories.
authors.affiliation: Department of Pharmacy, School of Health Sciences, University of Patras, Rion-Patras, 26500, Greece. geopamp@upatras.gr gdsotiro@upatras.gr.
authors.forename: G
authors.initials: G
authors.lastname: Pampalakis
authors.forename: E
authors.initials: E
authors.lastname: Zingkou
authors.forename: K
authors.initials: K
authors.lastname: Vekrellis
authors.forename: G
authors.initials: G
authors.lastname: Sotiropoulou
mesh_terms: D000818:Animals; D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D007610:Kallikreins / Q000172:deficiency / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D063065:Organophosphonates / Q000737:chemistry / Q000378:metabolism*; D000074163:Serine Peptidase Inhibitor Kazal-Type 5 / Q000172:deficiency / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
chemical_list: D015335:Molecular Probes; D063065:Organophosphonates; D000074163:Serine Peptidase Inhibitor Kazal-Type 5; C495972:Spink5 protein, mouse; D001710:Biotin; D007610:Kallikreins; C495973:Klk5 protein, mouse