title: Bioactivity-guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity.
abstract: Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human uterus carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
authors.affiliation: a Faculty of Art and Science, Department of Chemistry , Gaziosmanpasa University , Tokat , Turkey.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Yildiz
authors.affiliation: a Faculty of Art and Science, Department of Chemistry , Gaziosmanpasa University , Tokat , Turkey.
authors.forename: Ozkan
authors.initials: O
authors.lastname: Sen
authors.affiliation: a Faculty of Art and Science, Department of Chemistry , Gaziosmanpasa University , Tokat , Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Erenler
authors.affiliation: b Faculty of Natural Sciences, Department of Chemistry , Cankiri Karatekin University , Cankiri , Turkey.
authors.forename: Ibrahim
authors.identifier: http://orcid.org/0000-0001-8946-647X
authors.initials: I
authors.lastname: Demirtas
authors.affiliation: c Faculty of Art and Science, Department of Biology , Bingol University , Bingol , Turkey.
authors.forename: Lutfi
authors.initials: L
authors.lastname: Behcet
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D031105:Cynanchum / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D044949:Kaempferols; D009005:Monosaccharides; D010936:Plant Extracts; C511963:kaempferol-3-O-glucoside; C016527:isoquercitrin; D011794:Quercetin
keywords: Cynanchum acutum subsp. sibiricum; antiproliferative activity; flavonoids