title: A modified method for isolating mouse islets of an adequate quality, quantity, and purity.
abstract: Mouse islets are widely used in diabetes research. Thus an adequate quality, quantity, and purity of islets are needed for high-quality investigations. We performed a combination of filtration and density gradient separation and optimized many steps in the islet isolation procedure, including perfusion, digestion, and purification. Our results show that an increased quality, quantity, and purity of isolated islets can be achieved using these modifications. Moreover, this method can guarantee maximal recovery and purity of the isolated islets and is easy to perform with practice.
authors.affiliation: a Department of Hepatic Surgery, the First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, P.R. China.
authors.forename: Jiejie
authors.initials: J
authors.lastname: Xu
authors.affiliation: a Department of Hepatic Surgery, the First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, P.R. China.
authors.forename: Baogang
authors.initials: B
authors.lastname: Peng
authors.affiliation: b Department of Hepatobiliary Surgery, Meizhou People's Hospital, No. 63 Huangtang Road, Meizhou 514000, P.R. China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: b Department of Hepatobiliary Surgery, Meizhou People's Hospital, No. 63 Huangtang Road, Meizhou 514000, P.R. China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Xu
authors.affiliation: c Organ Transplant Center, the First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ma
authors.affiliation: b Department of Hepatobiliary Surgery, Meizhou People's Hospital, No. 63 Huangtang Road, Meizhou 514000, P.R. China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods* / Q000592:standards*; D002499:Centrifugation, Density Gradient / Q000379:methods; D005374:Filtration / Q000379:methods; D007515:Islets of Langerhans / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
keywords: density gradient separation; exocrine tissue; filtration; islet isolation; isolement d’îlots de Langerhans; séparation sur gradient de densité; tissue exocrine