title: Fibroblast Growth Factor 1-Transfected Adipose-Derived Mesenchymal Stem Cells Promote Angiogenic Proliferation.
abstract: The aim of this study was to investigate, for the first time, the effects of using adipose-derived mesenchymal stem cells (AD-MSCs) transfected with an episomal plasmid encoding fibroblast growth factor 1 (FGF1) (AD-MSCsFGF1), in providing the microenvironment required for angiogenic proliferation. The isolated rat AD-MSCs were positive for mesenchymal (CD29 and CD90) and negative for hematopoietic (CD34 and CD45) surface markers. Adipogenic and osteogenic differentiation of the AD-MSCs also occurred in the proper culture media. The presence of FGF1 in the conditioned medium from the AD-MSCsFGF1was confirmed by Western blotting. G418 and PCR were used for selection of transfected cells and confirmation of the presence of FGF1 mRNA, respectively. Treatment with the AD-MSCFGF1-conditioned medium significantly increased the NIH-3T3 cell proliferation and human umbilical vein endothelial cell (HUVEC) tube formation compared to conditioned medium from nontransfected AD-MSCs (p < 0.001). In conclusion, the AD-MSCsFGF1efficiently secreted functional FGF1, which promoted angiogenic proliferation. Using AD-MSCsFGF1may provide a useful strategy in cell therapy, which can merge the beneficial effects of stem cells with the positive biological effects of FGF1 in various disorders, especially tissue defects, neurodegenerative, cardiovascular and diabetes endocrine pathologies, which remain to be tested in preclinical and clinical studies.
authors.affiliation: 1 Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Hoseini
authors.affiliation: 1 Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Hamed
authors.initials: H
authors.lastname: Ghazavi
authors.affiliation: 2 Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Forouzanfar
authors.affiliation: 3 Department of Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Baratali
authors.initials: B
authors.lastname: Mashkani
authors.affiliation: 4 Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Ahmad
authors.initials: A
authors.lastname: Ghorbani
authors.affiliation: 1 Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Elahe
authors.initials: E
authors.lastname: Mahdipour
authors.affiliation: 1 Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Faezeh
authors.initials: F
authors.lastname: Ghasemi
authors.affiliation: 5 Neurocognitive Research Center, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Sadeghnia
authors.affiliation: 6 Metabolic Syndrome Research Center, Mashhad University of Medical Sciences , Mashhad, Iran .|7 Cardiovascular Research Center, Mashhad University of Medical Sciences , Mashhad, Iran .
authors.forename: Majid
authors.initials: M
authors.lastname: Ghayour-Mobarhan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D016220:Fibroblast Growth Factor 1 / Q000235:genetics*; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D041681:NIH 3T3 Cells; D018919:Neovascularization, Physiologic / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
chemical_list: D016220:Fibroblast Growth Factor 1
keywords: HUVEC tube formation; angiogenesis; cell proliferation; fibroblast growth factor 1; mesenchymal stem cells