title: Antiparasitic Treatment Induces an Improved CD8+T Cell Response in Chronic Chagasic Patients.
abstract: Chagas disease is a chronic infection caused byTrypanosoma cruzi,an intracellular protozoan parasite. Chronic chagasic patients (CCPs) have dysfunctional CD8+T cells that are characterized by impaired cytokine production, high coexpression of inhibitory receptors, and advanced cellular differentiation. Most patients diagnosed in the chronic phase of Chagas disease already exhibit heart involvement, and there is no vaccination that protects against the disease. Antiparasitic treatment is controversial as to its indication for this stage of the disease. There is a lack of biological markers to evaluate the effectiveness of antiparasitic treatment, and little is known about the effect of the treatment on CD8+T cells. Thus, the aim of the current study was to analyze the early effects of antiparasitic treatment on CD8+T cells from CCPs with asymptomatic clinical forms of disease. To evaluate the CD8+T cell subsets, expression of inhibitory receptors, and functionality of T cells in CCPs, PBMCs were isolated. The results showed that treatment of CCPs with the asymptomatic form of the disease induces an increase in the frequency of CD8+central memory T cells and terminal effector T cells, a decrease in the coexpression of inhibitory receptors, an improved Ag-specific CD8+T cell response exhibited by the individual production of IFN-γ or IL-2, and a multifunctional CD8+T cell profile of up to four functions (IFN-γ+IL-2+Perforin+Granzyme B+). These findings suggest that, in CCPs, antiparasitic treatment improved the quality of Ag-specific CD8+T cell responses associated with a decrease in inhibitory receptor coexpression, which could serve as biomarkers for monitoring the effectiveness of antiparasitic treatment.
authors.affiliation: Grupo Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, 110231 Bogota, Colombia.|Laboratorio de Parasitología Molecular, Facultad de Ciencias, Pontificia Universidad Javeriana, 110231 Bogota, Colombia.
authors.forename: Jose
authors.identifier: 0000-0002-1402-3452
authors.initials: J
authors.lastname: Mateus
authors.affiliation: Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones Científicas, 18016 Granada, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Pérez-Antón
authors.affiliation: Grupo Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, 110231 Bogota, Colombia.|Laboratorio de Parasitología Molecular, Facultad de Ciencias, Pontificia Universidad Javeriana, 110231 Bogota, Colombia.
authors.forename: Paola
authors.initials: P
authors.lastname: Lasso
authors.affiliation: Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones Científicas, 18016 Granada, Spain.
authors.forename: Adriana
authors.initials: A
authors.lastname: Egui
authors.affiliation: Facultad de Medicina, Pontificia Universidad Javeriana, 110231 Bogota, Colombia.
authors.forename: Nubia
authors.initials: N
authors.lastname: Roa
authors.affiliation: Hospital Virgen de la Arrixaca, 30008 Murcia, Spain; and.
authors.forename: Bartolomé
authors.identifier: 0000-0002-0110-603X
authors.initials: B
authors.lastname: Carrilero
authors.affiliation: Grupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, 111711 Bogota, Colombia.
authors.forename: John M
authors.initials: JM
authors.lastname: González
authors.affiliation: Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones Científicas, 18016 Granada, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Thomas
authors.affiliation: Laboratorio de Parasitología Molecular, Facultad de Ciencias, Pontificia Universidad Javeriana, 110231 Bogota, Colombia.
authors.forename: Concepción J
authors.initials: CJ
authors.lastname: Puerta
authors.affiliation: Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones Científicas, 18016 Granada, Spain; acuellar@javeriana.edu.co mclopez@ipb.csic.es.
authors.forename: Manuel C
authors.initials: MC
authors.lastname: López
authors.affiliation: Grupo Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, 110231 Bogota, Colombia; acuellar@javeriana.edu.co mclopez@ipb.csic.es.
authors.forename: Adriana
authors.identifier: 0000-0003-0834-8442
authors.initials: A
authors.lastname: Cuéllar
mesh_terms: D000328:Adult; D000368:Aged; D000977:Antiparasitic Agents / Q000627:therapeutic use*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014355:Chagas Disease / Q000188:drug therapy* / Q000276:immunology*; D002908:Chronic Disease; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000977:Antiparasitic Agents