title: Microfluidic-Nanofiber Hybrid Array for Screening of Cellular Microenvironments.
abstract: Cellular microenvironments are generally sophisticated, but crucial for regulating the functions of human pluripotent stem cells (hPSCs). Despite tremendous effort in this field, the correlation between the environmental factors-especially the extracellular matrix and soluble cell factors-and the desired cellular functions remains largely unknown because of the lack of appropriate tools to recapitulate in vivo conditions and/or simultaneously evaluate the interplay of different environment factors. Here, a combinatorial platform is developed with integrated microfluidic channels and nanofibers, associated with a method of high-content single-cell analysis, to study the effects of environmental factors on stem cell phenotype. Particular attention is paid to the dependence of hPSC short-term self-renewal on the density and composition of extracellular matrices and initial cell seeding densities. Thus, this combinatorial approach provides insights into the underlying chemical and physical mechanisms that govern stem cell fate decisions.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Ken-Ichiro
authors.initials: KI
authors.lastname: Kamei
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.|Department of Environmental Chemistry and Engineering, Graduate School of Interdisciplinary Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Mashimo
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Yoshioka
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Yumie
authors.initials: Y
authors.lastname: Tokunaga
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Christopher
authors.initials: C
authors.lastname: Fockenberg
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Terada
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Koyama
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Minako
authors.initials: M
authors.lastname: Nakajima
authors.affiliation: Department of Environmental Chemistry and Engineering, Graduate School of Interdisciplinary Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.
authors.forename: Teiko
authors.initials: T
authors.lastname: Shibata-Seki
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.|Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science, Kasuga, Tsukuba-shi, Ibaraki, 305-0821, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.affiliation: Department of Environmental Chemistry and Engineering, Graduate School of Interdisciplinary Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
authors.affiliation: Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah, 88400, Malaysia.
authors.forename: Siew-Eng
authors.initials: SE
authors.lastname: How
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.|Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
authors.forename: Motonari
authors.initials: M
authors.lastname: Uesugi
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto, 606-8501, Japan.|Ecole Normale Supérieure, CNRS-ENS-UPMC UMR 8640, 24 Rue Lhomond, Paris, 75005, France.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D060833:Cellular Microenvironment; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D057139:Nanofibers / Q000737:chemistry*
keywords: cellular microenvironments; embryonic stem cells; microfluidics; nanofibers; single-cell profiling