title: Enzymatic cascades for the stereo-complementary epimerisation of in situ generated epoxy alcohols.
abstract: The synthesis of optically pure secondary epoxy alcohols from racemic allylic alcohols using a single whole-cell biocatalyst of recombinant Escherichia coli coexpressing three oxidoreductases is described. The cascade involves the concurrent action of a styrene monooxygenase that catalyzes the formation of the chiral epoxy group, and two alcohol dehydrogenases that fulfil the epimerisation of the hydroxy group. Two sets of alcohol dehydrogenases were each applied to couple with styrene monooxygenase in order to realize the epimerisation in a stereo-complementary manner. Excellent enantio- and diastereo-selectivities were achieved for most of the 12 substrates.
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. wuzhl@cib.ac.cn and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China and Graduate University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. wuzhl@cib.ac.cn and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China and Graduate University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. wuzhl@cib.ac.cn and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. wuzhl@cib.ac.cn and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Wang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. wuzhl@cib.ac.cn and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
authors.forename: Zhong-Liu
authors.identifier: http://orcid.org/0000-0002-0189-5892
authors.initials: ZL
authors.lastname: Wu