title: Differences in Vvufgt and VvmybA1 Gene Expression Levels and Phenolic Composition in Table Grape (Vitis vinifera L.) 'Red Globe' and Its Somaclonal Variant 'Pink Globe'.
abstract: A novel 'Red Globe' (RG)-derived grape variety, 'Pink Globe' (PG), was described and registered as a new genotype, with earlier ripening and sweeter taste than those of RG. Microsatellite analysis revealed that PG and RG are undifferentiable; however, the PG VvmybA1c contains six single-nucleotide polymorphisms within the coding and noncoding region, possibly related to the reduced VvmybA1 expression levels. Conversely, HPLC-DAD-ESI-MS/MS analysis showed significantly lower anthocyanin content in PG skin than in RG skin, and PG had no detectable trihydroxylated anthocyanins. Total flavonols did not differ between the variants, although some quercetin derivate concentrations were lower in PG. HPLC-FLD analysis revealed slightly higher concentrations of epicatechin and a procyanidin dimer in PG seeds, although the antioxidant capacity of crude extracts from either variety did not differ significantly. These differences, particularly in monomeric anthocyanin content, can be attributed to altered activity of a MYB-type transcription factor, reducing Vvufgt expression.
authors.affiliation: Instrumental Analysis Department, Faculty of Pharmacy, University of Concepción , P.O. Box 160-C, Concepción, Chile.
authors.forename: Luis
authors.initials: L
authors.lastname: Bustamante
authors.affiliation: Instrumental Analysis Department, Faculty of Pharmacy, University of Concepción , P.O. Box 160-C, Concepción, Chile.
authors.forename: Vania
authors.initials: V
authors.lastname: Sáez
authors.affiliation: Instituto de Investigaciones Agropecuarias, INIA La Platina , Santa Rosa 11610 La Pintana, Santiago, Chile.
authors.forename: Patricio
authors.initials: P
authors.lastname: Hinrichsen
authors.affiliation: Instituto de Investigaciones Agropecuarias, INIA La Platina , Santa Rosa 11610 La Pintana, Santiago, Chile.
authors.forename: María H
authors.initials: MH
authors.lastname: Castro
authors.affiliation: Instrumental Analysis Department, Faculty of Pharmacy, University of Concepción , P.O. Box 160-C, Concepción, Chile.
authors.forename: Carola
authors.initials: C
authors.lastname: Vergara
authors.affiliation: Instrumental Analysis Department, Faculty of Pharmacy, University of Concepción , P.O. Box 160-C, Concepción, Chile.
authors.forename: Dietrich
authors.initials: D
authors.lastname: von Baer
authors.affiliation: Instrumental Analysis Department, Faculty of Pharmacy, University of Concepción , P.O. Box 160-C, Concepción, Chile.
authors.forename: Claudia
authors.identifier: http://orcid.org/0000-0002-1645-1610
authors.initials: C
authors.lastname: Mardones
mesh_terms: D005638:Fruit / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D027843:Vitis / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D010636:Phenols; D010940:Plant Proteins
keywords: HPLC-DAD-MS/MS; Pink Globe; Red Globe; Vitis vinifera; VvmybA1; Vvufgt; microsatellite; phenolic compounds