title: Effects of different concentrations of Platelet-rich Plasma and Platelet-Poor Plasma on vitality and differentiation of autologous Adipose tissue-derived stem cells.
abstract: BACKGROUND
  Autologous fat grafts and adipose-derived stem cells (ASCs) can be used to treat soft tissue defects. However, the results are inconsistent and sometimes comprise tissue resorption and necrosis. This might be due to insufficient vascularization. Platelet-rich plasma (PRP) is a source of concentrated autologous platelets. The growth factors and cytokines released by platelets can facilitate angiogenesis. The simultaneous use of PRP might improve the regeneration potential of fat grafts. The optimal ratio has yet to be elucidated. A byproduct of PRP preparation is platelet-poor plasma (PPP).
  
  
  OBJECTIVE
  In this study we investigated the influence of different concentrations of PRP on the vitality and differentiation of ASCs.
  
  
  METHODS
  We processed whole blood with the Arthrex Angel centrifuge and isolated ASCs from the same donor. We tested the effects of different PRP and PPP concentrations on the vitality using resazurin assays and the differentiation of ASCs using oil-red staining.
  
  
  RESULTS
  Both cell vitality and adipogenic differentiation increase to a concentration of 10% to 20% PRP. With a PRP concentration of 30% cell vitality and differentiation decrease.
  
  
  CONCLUSIONS
  Both PRP and PPP can be used to expand ASCs without xenogeneic additives in cell culture. A PRP concentration above 20% has inhibitory effects.
authors.forename: Oliver
authors.initials: O
authors.lastname: Felthaus
authors.forename: Lukas
authors.initials: L
authors.lastname: Prantl
authors.forename: Mona
authors.initials: M
authors.lastname: Skaff-Schwarze
authors.forename: Silvan
authors.initials: S
authors.lastname: Klein
authors.forename: Alexandra
authors.initials: A
authors.lastname: Anker
authors.forename: Marco
authors.initials: M
authors.lastname: Ranieri
authors.forename: Britta
authors.initials: B
authors.lastname: Kuehlmann
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002454:Cell Differentiation; D006801:Humans; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
keywords: Adipose tissue derived stem cells; platelet rich plasma; tissue engineering