title: Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system.
authors.affiliation: Department of Biomedical Engineering, State Key Laboratory of Natural and Biomimetic Drugs, College of Engineering, Peking University, Beijing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.affiliation: Beijing Viewsolid Biotech Co. Ltd, Beijing 100071, China.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Zhuang
authors.affiliation: Beijing Viewsolid Biotech Co. Ltd, Beijing 100071, China.
authors.forename: Xiongbing
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Biomedical Engineering, State Key Laboratory of Natural and Biomimetic Drugs, College of Engineering, Peking University, Beijing, China.
authors.forename: Bolun
authors.initials: B
authors.lastname: Wang
authors.affiliation: Division of Gastrointestinal Cancer Translational Research Laboratory, Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Fu-Cheng Road, Beijing, China.
authors.forename: Xian-Zi
authors.initials: XZ
authors.lastname: Wen
authors.affiliation: Division of Gastrointestinal Cancer Translational Research Laboratory, Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Fu-Cheng Road, Beijing, China.
authors.forename: Jia-Fu
authors.initials: JF
authors.lastname: Ji
authors.affiliation: Department of Biomedical Engineering, State Key Laboratory of Natural and Biomimetic Drugs, College of Engineering, Peking University, Beijing, China.|State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.|Collaborative Innovation Center for Cardiovascular Disorders, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Jianzhong Jeff
authors.initials: JJ
authors.lastname: Xi
mesh_terms: D000483:Alleles; D000818:Animals; D001360:Avidin / Q000378:metabolism*; D001483:Base Sequence; D001710:Biotin / Q000378:metabolism*; D000076987:CRISPR-Associated Protein 9 / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005821:Genetic Techniques; D006720:Homozygote; D019426:Integrases / Q000378:metabolism; D008822:Mice, Transgenic
chemical_list: D001360:Avidin; D001710:Biotin; D004247:DNA; C045073:Cre recombinase; D019426:Integrases; D000076987:CRISPR-Associated Protein 9