title: MicroRNA-124-3p affects proliferation, migration and apoptosis of bladder cancer cells through targeting AURKA.
abstract: OBJECTIVE
  The aim of this study was to establish the relationship between miR-124-3p and Aurora A kinase (AURKA) in bladder cancer (BC).
  
  
  METHODS
  The expressions of miR-124-3p and AURKA in BC tissues and cell lines were detected using RT-PCR and western blot. BC cells were transfected with miR-124-3p mimics and AURKA siRNA. After this cell proliferation, migration, cell cycle and apoptosis were measured using CCK-8, colony formation assay, wound healing assay and cytometry tests. The relationship between miR-124-3p and AURKA was confirmed with luciferase reporter assay. Mice xenograft models were constructed to examine the effects of AURKA on BC in vivo.
  
  
  RESULTS
  MiR-124-3p expression was significantly down-regulated in BC tissues and cell lines, while AURKA was significantly up-regulated compared to normal samples. MiR-124-3p targeted AURKA and decreased its expression. Transfection of miR-124-3p mimics and AURKA siRNA was shown to down-regulate BC cell proliferation and migration as well as induce cell apoptosis. As suggested by xenograft models, the inhibition of AURKA can effectively suppress tumor growth.
  
  
  CONCLUSIONS
  MiR-124-3p has significant impact on proliferation, migration and apoptosis of BC cells by targeting AURKA.
authors.affiliation: Cancer Cente, The First Hospital of Jilin University, Changchun, Jilin 130021, China.|Cancer Cente, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Cancer Cente, The First Hospital of Jilin University, Changchun, Jilin 130021, China.|Cancer Cente, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Tingge
authors.initials: T
authors.lastname: Sun
authors.affiliation: Cancer Cente, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ye
authors.affiliation: BASO Cell Science and Technology Co., Ltd., Zhuhai, Guangdong 519015, China.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Kong
authors.affiliation: Cancer Cente, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Haofan
authors.initials: H
authors.lastname: Jin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D064096:Aurora Kinase A / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; C576917:AURKA protein, human; D064096:Aurora Kinase A
keywords: AURKA; MicroRNA-124-3p; bladder cancer