title: Biomaterial surface proteomic signature determines interaction with epithelial cells.
abstract: Cells interact with biomaterials indirectly through extracellular matrix (ECM) proteins adsorbed onto their surface. Accordingly, it could be hypothesized that the surface proteomic signature of a biomaterial might determine its interaction with cells. Here, we present a surface proteomic approach to test this hypothesis in the specific case of biomaterial-epithelial cell interactions. In particular, we determined the surface proteomic signature of different biomaterials exposed to the ECM of epithelial cells (basal lamina). We revealed that the biomaterial surface chemistry determines the surface proteomic profile, and subsequently the interaction with epithelial cells. In addition, we found that biomaterials with surface chemistries closer to that of percutaneous tissues, such as aminated PMMA and aminated PDLLA, promoted higher selective adsorption of key basal lamina proteins (laminins, nidogen-1) and subsequently improved their interactions with epithelial cells. These findings suggest that mimicking the surface chemistry of natural percutaneous tissues can improve biomaterial-epithelial integration, and thus provide a rationale for the design of improved biomaterial surfaces for skin regeneration and percutaneous medical devices.
  
  
  
  Failure of most biomaterials originates from the inability to predict and control the influence of their surface properties on biological phenomena, particularly protein adsorption, and cellular behaviour, which subsequently results in unfavourable host response. Here, we introduce a surface-proteomic screening approach using a label-free mass spectrometry technique to decipher the adsorption profile of extracellular matrix (ECM) proteins on different biomaterials, and correlate it with cellular behaviour. We demonstrated that the way a biomaterial selectively interacts with specific ECM proteins of a given tissue seems to determine the interactions between the cells of that tissue and biomaterials. Accordingly, this approach can potentially revolutionize the screening methods for investigating the protein-cell-biomaterial interactions and pave the way for deeper understanding of these interactions.
authors.affiliation: Faculty of Dentistry, McGill University, Montreal, QC, Canada.
authors.forename: Mohamed-Nur
authors.initials: MN
authors.lastname: Abdallah
authors.affiliation: Faculty of Dentistry, McGill University, Montreal, QC, Canada; Craniofacial Stem Cells and Tissue Engineering Laboratory, McGill University, Montreal, QC, Canada.
authors.forename: Simon D
authors.initials: SD
authors.lastname: Tran
authors.affiliation: Faculty of Dentistry, McGill University, Montreal, QC, Canada; Craniofacial Stem Cells and Tissue Engineering Laboratory, McGill University, Montreal, QC, Canada; Faculty of Medicine, University of Jordan, Amman, Jordan.
authors.forename: Ghada
authors.initials: G
authors.lastname: Abughanam
authors.affiliation: Faculty of Dentistry, McGill University, Montreal, QC, Canada.
authors.forename: Marco
authors.initials: M
authors.lastname: Laurenti
authors.affiliation: Schulich Dentistry and Department of Biochemistry, University of Western Ontario, London, ON, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Zuanazzi
authors.affiliation: Faculty of Dentistry, McGill University, Montreal, QC, Canada.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Mezour
authors.affiliation: Schulich Dentistry and Department of Biochemistry, University of Western Ontario, London, ON, Canada.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Mining and Materials Engineering, McGill University, Montreal, QC, Canada.
authors.forename: Marta
authors.initials: M
authors.lastname: Cerruti
authors.affiliation: Schulich Dentistry and Department of Biochemistry, University of Western Ontario, London, ON, Canada.
authors.forename: Walter L
authors.initials: WL
authors.lastname: Siqueira
authors.affiliation: Faculty of Dentistry, McGill University, Montreal, QC, Canada. Electronic address: faleh.tamimimarino@mcgill.ca.
authors.forename: Faleh
authors.initials: F
authors.lastname: Tamimi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008297:Male; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000494:pharmacology; D040901:Proteomics; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D019904:Polymethyl Methacrylate
keywords: Cell interactions; Percutaneous devices; Proteins adsorption; Proteomics; Surface characterization