title: Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine.
abstract: Licorice has been used as herbal medicine and natural sweetener. Here, we prepared Glycyrrhiza uralensis water extract (GUWE) and investigated the effect of GUWE on the maturation and function of dendritic cells (DCs) and its adjuvant effect on DC-based vaccine. We observed that GUWE dose-dependently promoted DC maturation and cytokine secretion through TLR4 signaling pathway. The capacity of DC to stimulate allogenic splenocyte proliferation was also enhanced by GUWE treatment. Compared with control group, GUWE treated DCs pulsed with human papillomavirus (HPV)-16 E6/E7 peptides significantly inhibited the tumor growth in both early and late therapeutic groups. In early therapeutic group, the frequencies of induced regulatory T cells (iTregs: CD4+CD25-Fopx3+) and CD4+and CD8+T cells were significantly decreased and increased, respectively. HPV-16-specific CD8+T cell responses were significantly induced and negatively correlated with iTreg frequencies and tumor weight. These results indicated the immunoregulatory activities of licorice.
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Adila
authors.initials: A
authors.lastname: Aipire
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Key laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, 140 Xinhua South Road, Urumqi 830004, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: He
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Yelang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: XinJiang DingJu Biotech CO., LTD, 181 Xicai Road, Urumqi 830000, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: XinJiang DingJu Biotech CO., LTD, 181 Xicai Road, Urumqi 830000, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Feng
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Li
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.|Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, TX 77030, USA.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yang
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Jinyao
authors.initials: J
authors.lastname: Li
mesh_terms: D052685:Alphapapillomavirus / Q000276:immunology; D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000276:immunology; D002461:Cell Line, Transformed; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D005260:Female; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D047368:Tumor Burden / Q000187:drug effects / Q000276:immunology; D014867:Water / Q000737:chemistry
chemical_list: D019496:Cancer Vaccines; D009856:Oncogene Proteins, Viral; D010936:Plant Extracts; D014867:Water