title: Stress-sensitive arterial hypertension, haemodynamic changes and brain metabolites in hypertensive ISIAH rats: MRI investigation.
abstract: What is the central question of this study? Stress-sensitive arterial hypertension is considered to be controlled by changes in central and peripheral sympathetic regulating mechanisms, which eventually result in haemodynamic alterations and blood pressure elevation. Therefore, study of the early stages of development of hypertension is of particular interest, because it helps in understanding the aetiology of the disease. What is the main finding and its importance? Non-invasive in vivo investigation in ISIAH rats demonstrated that establishment of sustainable stress-sensitive hypertension is accompanied by a decrease in prefrontal cortex activity and mobilization of hypothalamic processes, with considerable correlations between haemodynamic parameters and individual metabolite ratios. The study of early development of arterial hypertension in association with emotional stress is of great importance for better understanding of the aetiology and pathogenesis of the hypertensive disease. Magnetic resonance imaging (MRI) was applied to evaluate the changes in haemodynamics and brain metabolites in 1- and 3-month-old inherited stress-induced arterial hypertension (ISIAH) rats (10 male rats) with stress-sensitive arterial hypertension and in control normotensive Wistar Albino Glaxo (WAG) rats (eight male rats). In the 3-month-old ISIAH rats, the age-dependent increase in blood pressure was associated with increased blood flow through the renal arteries and decreased blood flow in the lower part of the abdominal aorta. The renal vascular resistance in the ISIAH rats decreased during ageing, although at both ages it remained higher than the renal vascular resistance in WAG rats. An integral metabolome portrait demonstrated that development of hypertension in the ISIAH rats was associated with an attenuation of the excitatory and energetic activity in the prefrontal cortex, whereas in the WAG rats the opposite age-dependent changes were observed. In contrast, in the hypothalamus of 3-month-old ISIAH rats, an increase in energetic activity and prevalence of excitatory over inhibitory neurotransmitters was noticed. The blood flow through the main arteries showed a positive correlation with glutamate and glutamine levels in the hypothalamus and a negative correlation with the hypothalamic GABA level. The blood pressure values were positively correlated with hypothalamic choline levels. Thus, the early development of stress-sensitive hypertension in the ISIAH rats is accompanied by considerable changes both in brain metabolite ratios and in the parameters of blood flow through the main arteries.
authors.affiliation: Institute of Cytology and Genetics (SB RAS), Novosibirsk, Russian Federation.
authors.forename: A A
authors.identifier: 0000-0002-8807-2580
authors.initials: AA
authors.lastname: Seryapina
authors.affiliation: Institute of Cytology and Genetics (SB RAS), Novosibirsk, Russian Federation.
authors.forename: O B
authors.initials: OB
authors.lastname: Shevelev
authors.affiliation: Institute of Cytology and Genetics (SB RAS), Novosibirsk, Russian Federation.|Novosibirsk State University, Novosibirsk, Russian Federation.
authors.forename: M P
authors.initials: MP
authors.lastname: Moshkin
authors.affiliation: Institute of Cytology and Genetics (SB RAS), Novosibirsk, Russian Federation.|Novosibirsk State University, Novosibirsk, Russian Federation.
authors.forename: A L
authors.initials: AL
authors.lastname: Markel
authors.affiliation: Institute of Cytology and Genetics (SB RAS), Novosibirsk, Russian Federation.
authors.forename: A E
authors.initials: AE
authors.lastname: Akulov
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D001921:Brain / Q000378:metabolism / Q000503:physiopathology*; D004195:Disease Models, Animal; D006439:Hemodynamics / Q000502:physiology*; D006973:Hypertension / Q000378:metabolism / Q000503:physiopathology*; D007031:Hypothalamus / Q000378:metabolism / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012077:Renal Artery / Q000378:metabolism / Q000503:physiopathology*; D013312:Stress, Physiological / Q000502:physiology*; D013315:Stress, Psychological / Q000378:metabolism / Q000503:physiopathology
chemical_list: D018377:Neurotransmitter Agents
keywords: ISIAH rats; blood flow; brain metabolites; stress-sensitive arterial hypertension