title: Human umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease through the regulation of 15-LOX-1 in macrophages.
abstract: OBJECTIVE
  To investigate the role of human umbilical cord mesenchymal stem cells (hucMSCs) in the treatment of dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD).
  
  
  RESULTS
  ICG-hucMSCs homed to colon tissues of IBD mice 12 h after injection. The injection of hucMSCs significantly relieved the IBD symptoms and inflammatory cell infiltration. The expression of IL-10 gene increased while those of 15-LOX-1, TNF-α, IL-6, IL-1β, and IP-10 genes decreased in colon tissues and spleens of hucMSCs-treated mice. The activation of STAT3 was inhibited in colon tissues and spleens of IBD mice that were treated with hucMSCs. In addition, the percentage of macrophages decreased in colon tissues and spleens of hucMSCs-treated IBD mice. Moreover, we provided evidence that in vitro co-culture with hucMSCs inhibited the expression of 15-LOX-1, IL-6 and p-STAT3 in mouse enterocoelia macrophages.
  
  
  CONCLUSIONS
  HucMSCs alleviate DSS-induced IBD through the modulation of 15-LOX-1 in macrophages.
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Fei
authors.identifier: http://orcid.org/0000-0001-5840-4436
authors.initials: F
authors.lastname: Mao
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Yunbing
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu, People's Republic of China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Zhaoji
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qian
authors.affiliation: Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China. icls@ujs.edu.cn.
authors.forename: Wenrong
authors.initials: W
authors.lastname: Xu
mesh_terms: D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000378:metabolism*; D003106:Colon / Q000378:metabolism; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D016264:Dextran Sulfate / Q000009:adverse effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000601:surgery*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D050796:STAT3 Transcription Factor / Q000032:analysis / Q000378:metabolism; D015398:Signal Transduction; D013154:Spleen / Q000378:metabolism
chemical_list: D016207:Cytokines; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D016264:Dextran Sulfate; D001093:Arachidonate 15-Lipoxygenase
keywords: Inflammatory bowel disease; Macrophages; Mesenchymal stem cells 15-LOX-1; hucMSCs