title: A yellowish-green-light-controllable nitric oxide donor based on N-nitrosoaminophenol applicable for photocontrolled vasodilation.
abstract: Nitric oxide (NO) has been known as a gaseous chemical mediator, which modulates several physiological functions. Spatial and temporal control of NO release facilitates further study and medical application of NO. Herein, we report design and synthesis of a novel NO donor, NO-Rosa. NO-Rosa has a rosamine moiety, which absorbs yellowish green light. Upon irradiation with yellowish green light (530-590 nm), NO is released from NO-Rosa, presumably via photoinduced electron transfer from the N-nitrosoaminophenol moiety to the rosamine moiety. NO release from NO-Rosa was detected by ESR spin trapping and a NO fluorescent probe. Cellular NO release control was achieved in HEK293 cells using a NO fluorescent probe, DAF-FM DA. Furthermore, temporally controlled NO-induced vasodilation was demonstrated by treatment of a rat aortic strip with NO-Rosaex vivo and irradiation by yellowish green light. NO-Rosa is expected to be utilized for further study of NO-related physiological functions, utilizing its ability of spatiotemporal release of NO as a photocontrollable compound with harmless yellowish-green light.
authors.affiliation: Graduate School of Pharmaceutical Science, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. deco@phar.nagoya-cu.ac.jp.
authors.forename: Hana
authors.initials: H
authors.lastname: Okuno
authors.affiliation: Graduate School of Pharmaceutical Science, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. deco@phar.nagoya-cu.ac.jp.
authors.forename: Naoya
authors.initials: N
authors.lastname: Ieda
authors.affiliation: Graduate School of Pharmaceutical Science, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. deco@phar.nagoya-cu.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hotta
authors.affiliation: Graduate School of Pharmaceutical Science, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. deco@phar.nagoya-cu.ac.jp.
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Kawaguchi
authors.affiliation: Graduate School of Pharmaceutical Science, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. deco@phar.nagoya-cu.ac.jp.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Graduate School of Pharmaceutical Science, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. deco@phar.nagoya-cu.ac.jp.
authors.forename: Hidehiko
authors.identifier: http://orcid.org/0000-0001-8435-4401
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000627:Aminophenols / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D015394:Molecular Structure; D020030:Nitric Oxide Donors / Q000737:chemistry / Q000378:metabolism*; D009603:Nitroso Compounds / Q000737:chemistry / Q000378:metabolism*; D055668:Photochemical Processes
chemical_list: D000627:Aminophenols; D005456:Fluorescent Dyes; D020030:Nitric Oxide Donors; D009603:Nitroso Compounds