title: A new fluorescence turn-on nanobiosensor for the detection of micro-RNA-21 based on a DNA-gold nanocluster.
abstract: In this study, DNA/gold nanoclusters (AuNCs) were used to develop an AuNC-based turn-on fluorescence probe for the analysis of mi-RNA-21, which is a potential screening biomarker for cancer detection. AuNCs on a DNA scaffold were prepared through a one-pot wet-chemical route and evaluated by transmission electron microscopy and dynamic light scattering. Experiments revealed that the fluorescence intensity of the DNA-AuNCs showed a gradual increase with the addition of the target species in a concentration range from 1pM to 10 nM. The method had a detection limit of 0.7 pM and was able to discriminate the target species from mismatched mi-RNAs very efficiently. The method was used for the determination of mi-RNA spiked human plasma samples, and was evaluated as a promising nanobiosensor for application in the selective detection of mi-RNA in various biomedical and clinical tests.
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran. Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hosseini
authors.forename: Elnaz
authors.initials: E
authors.lastname: Ahmadi
authors.forename: Yasaman-Sadat
authors.initials: YS
authors.lastname: Borghei
authors.forename: Mohammad
authors.initials: M
authors.lastname: Reza Ganjali
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D006046:Gold; D004247:DNA