title: Analytical Biases Associated with GC-Content in Molecular Evolution.
abstract: Molecular evolution is being revolutionized by high-throughput sequencing allowing an increased amount of genome-wide data available for multiple species. While base composition summarized by GC-content is one of the first metrics measured in genomes, its genomic distribution is a frequently neglected feature in downstream analyses based on DNA sequence comparisons. Here, we show how base composition heterogeneity among loci and taxa can bias common molecular evolution analyses such as phylogenetic tree reconstruction, detection of natural selection and estimation of codon usage. We then discuss the biological, technical and methodological causes of these GC-associated biases and suggest approaches to overcome them.
authors.affiliation: Department of Ecology and Evolution, University of Lausanne Lausanne, Switzerland.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Romiguier
authors.affiliation: Department of Ecology and Evolution, University of Lausanne Lausanne, Switzerland.
authors.forename: Camille
authors.initials: C
authors.lastname: Roux
keywords: GC-content; biased gene conversion; codon usage bias; methodological biases; phylogeny; positive selection