title: Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.
abstract: Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
authors.forename: Dongli
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, MI 48109, USA.
authors.forename: Weibin
authors.initials: W
authors.lastname: Zhou
authors.affiliation: College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA; Department of Molecular and Cell Biology, University of Arizona, 1007 E. Lowell Street, Tucson. AZ 85721, USA.
authors.forename: Leilei
authors.initials: L
authors.lastname: Peng
keywords: (110.0110) Imaging systems; (170.2520) Fluorescence microscopy; (170.6280) Spectroscopy, fluorescence and luminescence; (180.6900) Three-dimensional microscopy