title: Production of rice straw hydrolysis enzymes by the fungi Trichoderma reesei and Humicola insolens using rice straw as a carbon source.
abstract: Rice straw was evaluated as a carbon source for the fungi, Trichoderma reesei and Humicola insolens, to produce enzymes for rice straw hydrolysis. The enzyme activity of T. reesei and H. insolens cultivated in medium containing non-treated rice straw were almost equivalent to the enzyme of T. reesei cultivated in Avicel medium, a form of refined cellulose. The enzyme activity of T. reesei cultivated in medium containing NH4OH-treated rice straw was 4-fold higher than enzyme from cultures grown in Avicel medium. In contrast, H. insolens enzyme from cultures grown in NH4OH-treated rice straw had significantly lower activity compared with non-treated rice straw or Avicel. The combined use of T. reesei and H. insolens enzymes resulted in a significant synergistic enhancement in enzymatic activity. Our data suggest that rice straw is a promising low-cost carbon source for fungal enzyme production for rice straw hydrolysis.
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Chemistry and Lifescience, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kogo
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Chemistry and Lifescience, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Chemistry and Lifescience, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Koganei
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Chemistry and Lifescience, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Chemistry and Lifescience, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Chemistry and Lifescience, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogihara
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Chemistry and Lifescience, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: kasumi@brs.nihon-u.ac.jp.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Kasumi
mesh_terms: D002244:Carbon; D002480:Cellulase; D002482:Cellulose; D006868:Hydrolysis; D012275:Oryza; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D002244:Carbon; D002482:Cellulose; D002480:Cellulase
keywords: Biomass degrading enzymes; Humicola insolens; Rice straw; Synergistic effect; Trichoderma reesei