title: Surface Engineering of Nanoparticles to Create Synthetic Antibodies.
abstract: Surface engineering of nanoparticles has recently emerged as a promising technique for synthetic molecular recognition of biological analytes. In particular, the use of synthetic heteropolymers adsorbed onto the surface of a nanoparticle can yield selective detection of a molecular target. Synthetic molecular recognition has unique advantages in leveraging the photostability, versatility, and exceptional chemical stability of nanomaterials. In particular, single-walled carbon nanotubes (SWNT) exhibit a large Stokes shift and near infrared emission for maximum biological sample transparency. Optical biosensors with high signal transduction and molecular specificity can be synthesized with amphiphilic heteropolymers grafted to SWNT, and discovered by high-throughput screening. Herein, we describe the development and the characterization of surface-engineered nanoparticles, or "synthetic antibodies," for protein detection.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
authors.forename: Linda
authors.initials: L
authors.lastname: Chio
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
authors.forename: Darwin
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA. landry@berkeley.edu.|Landry Lab, California Institute for Quantitative Biosciences, QB3, University of California, #3220, 476 Stanley Hall, Berkeley, CA, 94720, USA. landry@berkeley.edu.
authors.forename: Markita
authors.initials: M
authors.lastname: Landry
mesh_terms: D000327:Adsorption; D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D008856:Microscopy, Fluorescence; D037742:Nanotubes, Carbon; D013499:Surface Properties
chemical_list: D000906:Antibodies; D037742:Nanotubes, Carbon
keywords: Carbon nanotubes; DNA aptamers; Infrared fluorescence microscopy; Nanomaterials; Protein detection; Sensors; Synthetic antibodies