title: Three-phase partitioning for efficient extraction and separation of polysaccharides from Corbicula fluminea.
abstract: Three-phase partitioning (TPP), which is a simple, efficient, and green bioseparation technique, was used to extract and separate polysaccharides from Corbicula fluminea (CFPS). The optimal parameters with a high yield of 9.32% were as follows: mass fraction of (NH4)2SO4, 20.0% (w/v); amount of t-butanol, 9.8mL; temperature, 35.3°C; extraction time, 30min; and pH 6.0. The purified CFPS after TPP consisted of d-glucose, d-glucosamine, and d-mannose in a molar ratio of 57.1: 5.6:1.0 with high purity (86.5%) and different molecular weights (1311.1 and 41.5-92.8kDa). Amino acid analysis, UV-vis absorption and Fourier transform-infrared spectra indicated that the purified CFPS was a proteoglycan with O-glycosidic bonds. Moreover, the purified CFPS possessed strong free-radical scavenging abilities and antioxidant activities in vitro. The obtained Trolox equivalent antioxidant capacity and ferric-reducing ability of plasma values were 95.01μmol Trolox/g sample and 38.30μmol Fe2+/g sample, respectively.
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: jkyan27@ujs.edu.cn.
authors.forename: Jing-Kun
authors.initials: JK
authors.lastname: Yan
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yao-Yao
authors.initials: YY
authors.lastname: Wang
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Qiu
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Shao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D049874:Corbicula / Q000737:chemistry*; D055772:Green Chemistry Technology; D008970:Molecular Weight; D011134:Polysaccharides / Q000302:isolation & purification*; D011509:Proteoglycans / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D011509:Proteoglycans
keywords: Characterization; Corbicula fluminea; Extraction yield; Optimization; Polysaccharide; Three-phase partitioning