title: Separation of Oligosaccharides from Lotus Seeds via Medium-pressure Liquid Chromatography Coupled with ELSD and DAD.
abstract: Lotus seeds were identified by the Ministry of Public Health of China as both food and medicine. One general function of lotus seeds is to improve intestinal health. However, to date, studies evaluating the relationship between bioactive compounds in lotus seeds and the physiological activity of the intestine are limited. In the present study, by using medium pressure liquid chromatography coupled with evaporative light-scattering detector and diode-array detector, five oligosaccharides were isolated and their structures were further characterized by electrospray ionization-mass spectrometry and gas chromatography-mass spectrometry. In vitro testing determined that LOS3-1 and LOS4 elicited relatively good proliferative effects on Lactobacillus delbrueckii subsp. bulgaricus. These results indicated a structure-function relationship between the physiological activity of oligosaccharides in lotus seeds and the number of probiotics applied, thus providing room for improvement of this particular feature. Intestinal probiotics may potentially become a new effective drug target for the regulation of immunity.
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Teagasc Food Research Centre, Food Chemistry and Technology Department, Moorepark, Fermoy, Co.Cork, Ireland.|Institute of Food Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Zhichang
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Teagasc Food Research Centre, Food Chemistry and Technology Department, Moorepark, Fermoy, Co.Cork, Ireland.
authors.forename: Song
authors.initials: S
authors.lastname: Miao
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Institute of Food Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Zebin
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Institute of Food Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Institute of Food Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Institute of Food Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Baodong
authors.initials: B
authors.lastname: Zheng
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Xiao
mesh_terms: D002236:Carbohydrate Conformation; D008401:Gas Chromatography-Mass Spectrometry; D000070116:Lotus / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009844:Oligosaccharides