title: Investigating Structure and Dynamics of Atg8 Family Proteins.
abstract: Atg8 family members were the first autophagy-related proteins to be investigated in structural detail and continue to be among the best-understood molecules of the pathway. In this review, we will first provide a concise outline of the major methods that are being applied for structural characterization of these proteins and the complexes they are involved in. This includes a discussion of the strengths and limitations associated with each method, along with guidelines for successful adoption to a specific problem. Subsequently, we will present examples illustrating the application of these techniques, with a particular focus on the complementarity of information they provide.
authors.affiliation: Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich, Jülich, Germany.
authors.forename: O H
authors.initials: OH
authors.lastname: Weiergräber
authors.affiliation: Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich, Jülich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schwarten
authors.affiliation: Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich, Jülich, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Strodel
authors.affiliation: Institute of Complex Systems, ICS-6 (Structural Biochemistry), Forschungszentrum Jülich, Jülich, Germany; Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany. Electronic address: d.willbold@fz-juelich.de.
authors.forename: D
authors.initials: D
authors.lastname: Willbold
mesh_terms: D000071190:Autophagy-Related Protein 8 Family / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation
chemical_list: D000071190:Autophagy-Related Protein 8 Family
keywords: Atg8 family; Autophagy; Molecular dynamics simulation; NMR spectroscopy; Structural biology; X-ray crystallography