title: Engineering a vascularised 3D in vitro model of cancer progression.
abstract: The hallmark of tumours is the ability of cancerous cells to promote vascular growth, to disseminate and invade to distant organs. The metastatic process is heavily influenced by the extracellular matrix (ECM) density and composition of the surrounding tumour microenvironment. These microenvironmental cues, which include hypoxia, also regulate the angiogenic processes within a tumour, facilitating the spread of cancer cells. We engineered compartmentalized biomimetic colorectal tumouroids with stromal surrounds that comprised a range of ECM densities, composition and stromal cell populations. Recapitulating tissue ECM composition and stromal cell composition enhanced cancer cell invasion. Manipulation of ECM density was associated with an altered migration pattern from glandular buds (cellular aggregates) to epithelial cell sheets. Laminin appeared to be a critical component in regulating endothelial cell morphology and vascular network formation. Interestingly, the disruption of vascular networks by cancer cells was driven by changes in expression of several anti-angiogenic genes. Cancer cells cultured in our biomimetic tumouroids exhibited intratumoural heterogeneity that was associated with increased tumour invasion into the stroma. These findings demonstrate that our 3D in vitro tumour model exhibits biomimetic attributes that may permit their use in studying microenvironment clues of tumour progression and angiogenesis.
authors.affiliation: UCL Institute of Orthopaedics and Musculoskeletal Sciences, UCL Division of Surgery and Interventional Science, Stanmore Campus, HA7 4LP, UK.|UCL Division of Surgery and Interventional Science, Royal Free Campus, London, NW3 2QG, UK.
authors.forename: Tarig
authors.initials: T
authors.lastname: Magdeldin
authors.affiliation: UCL Division of Surgery and Interventional Science, Royal Free Campus, London, NW3 2QG, UK.
authors.forename: Víctor
authors.initials: V
authors.lastname: López-Dávila
authors.affiliation: UCL Institute of Orthopaedics and Musculoskeletal Sciences, UCL Division of Surgery and Interventional Science, Stanmore Campus, HA7 4LP, UK.
authors.forename: Judith
authors.initials: J
authors.lastname: Pape
authors.affiliation: Sartorius Stedim Biotech, Royston, Herts, SG8 5WY, UK.
authors.forename: Grant W W
authors.initials: GW
authors.lastname: Cameron
authors.affiliation: UCL Division of Surgery and Interventional Science, Royal Free Campus, London, NW3 2QG, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Emberton
authors.affiliation: UCL Division of Surgery and Interventional Science, Royal Free Campus, London, NW3 2QG, UK.
authors.forename: Marilena
authors.initials: M
authors.lastname: Loizidou
authors.affiliation: UCL Institute of Orthopaedics and Musculoskeletal Sciences, UCL Division of Surgery and Interventional Science, Stanmore Campus, HA7 4LP, UK.
authors.forename: Umber
authors.initials: U
authors.lastname: Cheema
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D008954:Models, Biological; D009369:Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D023822:Tissue Engineering; D059016:Tumor Microenvironment