title: Investigating parylene-HT as a substrate for human cell patterning.
abstract: We demonstrate, for the first time, how parylene-HT on SiO2 substrates can be used as a human cell patterning platform. We demonstrate this platform with hNT astrocytes, derived from the human NTera2.D1 cell line. We show how hNT astrocytes are attracted to Parylene-HT and repelled by the SiO2 and are shown to adopt a similar morphology as that attained on standard tissue culture polystyrene. Furthermore, parylene-HT was capable of patterning the astrocytes achieving a ratio of 8:1 for cells on parylene compared to SiO2. Thus, as parylene-HT has similar physical properties to parylene-C with the addition of UV and thermal resistance, parylene-HT represents a desirable alternative substrate for human cell patterning.
authors.forename: Brad J
authors.initials: BJ
authors.lastname: Raos
authors.forename: E Scott
authors.initials: ES
authors.lastname: Graham
authors.forename: Alan F
authors.initials: AF
authors.lastname: Murray
authors.forename: M Cather
authors.initials: MC
authors.lastname: Simpson
authors.forename: Charles P
authors.initials: CP
authors.lastname: Unsworth
mesh_terms: D001253:Astrocytes / Q000166:cytology / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D012822:Silicon Dioxide / Q000737:chemistry*; D014992:Xylenes / Q000737:chemistry / Q000494:pharmacology
chemical_list: D011108:Polymers; D014992:Xylenes; C011055:parylene; D012822:Silicon Dioxide