title: Antioxidant capacity of Typha angustifolia extracts and two active flavonoids.
abstract: BACKGROUND
  The pollen of Typha angustifolia L. (Typhaceae) has been used as a traditional Chinese medicine for improving the microcirculation and promoting wound healing. Flavonoids are the main constituent in the plant, but little is known about the antioxidant activity of the principal constituent of the pollen in detail.
  
  
  OBJECTIVE
  To assess the antioxidant activities of ethanol and water extracts and two constituents of the pollen.
  
  
  METHODS
  Plant material (1 g) was extracted by 95% ethanol and water (10 mL × 2, 1 h each), respectively. The extracted activities (0.8-2.6 mg/mL) were measured by DPPH and the reducing activity of ferric chloride (1.7-2.6 mg/mL). Typhaneoside and isorhamnetin-3-O-neohesperidoside (I3ON) (2.8-70 μmol/L) were investigated on the relationship between NO, MDA and SOD in HUVECs treated with 100 μg/mL of LPS for 24 h.
  
  
  RESULTS
  Nine compounds were identified by UPLC-MS. Ethanol extract showed IC50values in DPPH (39.51 ± 0.72) and Fe3+reducing activity (82.76 ± 13.38), higher than the water extract (50.85 ± 0.74) and (106.33 ± 6.35), respectively. Typhaneoside and I3ON promoted cell proliferation at the respective concentration range of 2.8 to 70 μmol/L (p < 0.01). This two compounds decreased MDA (1.91 ± 0.10, 1.80 ± 0.34, p < 0.05) and NO levels (14.64 ± 0.08, 13.10 ± 0.88, p < 0.01), respectively, and increased SOD level (22.94 ± 2.48, 23.57 ± 2.38, p < 0.01) at the concentration of 70 μmol/L compared with LPS group.
  
  
  CONCLUSIONS
  The constituents from Typha angustifolia could be a novel therapeutic strategy for LPS-induced inflammation.
authors.affiliation: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization , Nanjing University of Chinese Medicine , Nanjing , China.
authors.forename: Peidong
authors.initials: P
authors.lastname: Chen
authors.affiliation: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization , Nanjing University of Chinese Medicine , Nanjing , China.
authors.forename: Yudan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization , Nanjing University of Chinese Medicine , Nanjing , China.
authors.forename: Beihua
authors.initials: B
authors.lastname: Bao
authors.affiliation: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization , Nanjing University of Chinese Medicine , Nanjing , China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization , Nanjing University of Chinese Medicine , Nanjing , China.
authors.forename: Anwei
authors.initials: A
authors.lastname: Ding
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D032401:Typhaceae
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids
keywords: Typha angustifolia; antioxidant; human umbilical vein endothelial cells; lipopolysaccharide