title: Anti Japanese encephalitis virus IgM positivity among patients with acute encephalitic syndrome admitted to different hospitals from all over Nepal.
abstract: The Japanese encephalitis virus (JEV) infection is one of the major public health problems in Nepal because of its increasing disease morbidity and mortality. The main purpose of this study was to determine the anti-JEV IgM positivity among acute encephalitis syndromic cases from all over Nepal. The present study was conducted at National Public Health Laboratory, Kathmandu, Nepal from April 2015 to October 2015. A total of 671 (418 CSF and 253 serum) samples were collected from 625 patients with acute encephalitic syndrome, admitted to different hospitals from all over Nepal. IgM antibody capture enzyme linked immunosorbent assay (ELISA) was used for the detection of anti-JEV IgM positive cases. The rate of anti-JEV IgM positivity was found to be 21.12%. The majority of positive cases (50%) were from the age group below 15 years, with the highest numbers of cases occurring in September (55.30%). Among all the anti-JEV IgM positive cases, higher numbers of cases were males. Geographically, the highest numbers of anti-JEV IgM positive cases were recorded from Terai region. Similarly, largest numbers of anti-JEV IgM positive cases were reported from Kailai district followed by those from Kanchanpur. However, anti-JEV IgM positive cases were also reported from hill districts. Continuation of active surveillance and vector control measures, proper management of diagnostic facilities and expanded program of immunization in JE endemic areas should be strongly emphasized to reduce the endemicity of the disease.
authors.affiliation: Department of Microbiology, St. Xavier's College, Maitighar, Kathmandu, Nepal.
authors.forename: Anupama
authors.initials: A
authors.lastname: Bhattarai
authors.affiliation: Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal.
authors.forename: Narayan Dutt
authors.identifier: http://orcid.org/0000-0002-7577-2633
authors.initials: ND
authors.lastname: Pant
authors.affiliation: Department of Microbiology, GoldenGate International College, Battisputali, Kathmandu, Nepal.
authors.forename: Krishus
authors.initials: K
authors.lastname: Nepal
authors.affiliation: National Public Health Laboratory, Teku, Kathmandu, Nepal.
authors.forename: Sailaja
authors.initials: S
authors.lastname: Adhikari
authors.affiliation: National Public Health Laboratory, Teku, Kathmandu, Nepal.
authors.forename: Mukunda
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, St. Xavier's College, Maitighar, Kathmandu, Nepal.
authors.forename: Pramila
authors.initials: P
authors.lastname: Parajuli
mesh_terms: D000071072:Acute Febrile Encephalopathy / Q000097:blood / Q000453:epidemiology* / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D062306:Geography, Medical; D006760:Hospitalization; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009390:Nepal / Q000453:epidemiology; D055815:Young Adult
chemical_list: D007075:Immunoglobulin M