title: Elevated Expression of Immunoreceptor Tyrosine-Based Inhibitory Motif (TIGIT) on T Lymphocytes is Correlated with Disease Activity in Rheumatoid Arthritis.
abstract: BACKGROUND It is well known that lymphocytes play an important role in rheumatoid arthritis (RA). T cell immunoreceptors with immunoglobulin (Ig) and immunoreceptor tyrosine-based inhibitory motif (TIGIT) have immunosuppressive co-stimulatory molecules that mediate inhibitory effects, but their roles in RA are poorly understood. MATERIAL AND METHODS Were recruited 76 patients with RA and 33 healthy controls (HC). Clinical manifestations, laboratory measurements, physical examination, and medical history of RA patients were recorded. The expression of TIGIT on CD3+ T lymphocytes, B lymphocytes, monocytes, neutrophils, CD3+CD4+ T lymphocytes, and CD3+CD8+ T lymphocytes was determined using flow cytometry. The expression of TIGIT on T lymphocytes in patients with RA was further analyzed to investigate its correlations with markers of autoimmune response, inflammation, and disease activity in RA. RESULTS Compared with HC, the expression levels of TIGIT on CD3+CD4+ T lymphocytes and CD3+CD8+ T lymphocytes were significantly increased in patients with RA (P < 0.01). The frequency of TIGIT-expressing CD3+CD4+ T lymphocytes was positively correlated with RF, increased ACPA, ESR, and CRP levels. The frequency of TIGIT-expressing CD3+CD8+ T lymphocytes was positively correlated with RF and ESR levels. Furthermore, the expression level of TIGIT on CD3+CD4+ T lymphocytes was positively correlated with the DAS28 score in RA. CONCLUSIONS The expression levels of TIGIT on T lymphocytes were elevated and correlated with disease activity in RA.
authors.affiliation: Department of Clinical laboratory, The 1st Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.affiliation: College of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Zhen
authors.initials: Z
authors.lastname: Deng
authors.affiliation: College of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Chuxin
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Lulu
authors.initials: L
authors.lastname: Zeng
authors.affiliation: College of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Jianqing
authors.initials: J
authors.lastname: Ye
authors.affiliation: College of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Medicine, The 1st Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Clinical Laboratory Medicine, The 1st Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Zikun
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory Medicine, The 1st Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Junming
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D001323:Autoantibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000097:blood / Q000276:immunology*; D012720:Severity of Illness Index
chemical_list: D001323:Autoantibodies; D011971:Receptors, Immunologic; C534712:TIGIT protein, human