title: Identification of two transcription factors activating the expression of OsXIP in rice defence response.
abstract: Xylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown.
  
  
  
  Herbivore infestation, wounding and methyl jasmonate (MeJA) treatment enhanced mRNA levels and protein levels of OsXIP. By analyzing different 5' deletion mutants of OsXIP promoter exposed to rice brown planthopper Nilaparvata lugens stress, a 562 bp region (-1451 - -889) was finally identified as the key sequence for the herbivores stress response. Using yeast one-hybrid screening, coupled with chromatin immunoprecipitation analysis, a basic helix-loop-helix protein (OsbHLH59) and an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor OsERF71 directly binding to the 562 bp key sequence to activate the expression of OsXIP were identified, which is further supported by transient expression assay. Moreover, transcriptional analysis revealed that mechanical wounding and treatment with MeJA resulted in an obvious increase in transcript levels of OsbHLH59 and OsERF71 in root and shoot tissues.
  
  
  
  Our data shows that two proteins as direct transcriptional activators of OsXIP responding to stress were identified. These results reveal a coordinated regulatory mechanism of OsXIP, which may probably be involved in defence responses via a JA-mediated signaling pathway.
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Cixi Agricultural Technology Promotion Center, Cixi, 315300, China.
authors.forename: Guozeng
authors.initials: G
authors.lastname: Rong
authors.affiliation: The Institute of Rural Development and Information Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Hou
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Dean
authors.initials: D
authors.lastname: Jiang
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, 310058, China. xyweng@zju.edu.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Weng
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D006430:Hemiptera / Q000472:pathogenicity / Q000502:physiology; D012275:Oryza / Q000469:parasitology* / Q000502:physiology*; D010935:Plant Diseases / Q000469:parasitology; D010940:Plant Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000502:physiology; D043325:Xylan Endo-1,3-beta-Xylosidase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D043325:Xylan Endo-1,3-beta-Xylosidase
keywords: 5’ deletion; OsERF71; OsbHLH59; Plant defence; Rice xylanase inhibitor; Transcriptional regulation