title: miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line.
abstract: This study was designed to investigate the role of miR-183 in modulating cell growth and apoptosis of tongue squamous cell carcinoma SCC25 cell line. Human squamous epithelial cell and squamous cell carcinoma cell line SCC25 was used, and miR-183 was inhibited. Cell growth, colony formation, and apoptotic rate, as well as the expression of caspase 3 and BCL-xL, were detected. Results showed that miR-183 was significantly overexpressed in the SCC25 cell line when compared with normal control. The miR-183 inhibitor reduced cell growth and colony formation, while the apoptosis percentage was significantly increased. The expression of activated caspase 3 and BCL-xL was obviously up- and downregulated in siRNA-transfected cells, respectively. In conclusion, miR-183 contributed to cell growth and proliferation, and suppressed cell apoptosis in SCC25 cells. Therefore, miR-183 might serve as a therapeutic target in tongue squamous cell carcinoma (TSCC).
authors.affiliation: Oral and Maxillofacial Surgery, The Central Hospital of Zhengzhou, University of Zhengzhou, Zhengzhou, Henan, P.R. China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Yan
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Cai
authors.forename: Yu
authors.initials: Y
authors.lastname: Feng
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C533449:MIRN183 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering