title: Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization.
abstract: The data herein is in support of our research article by McDougall et al. (2017) [1], in which we used our zebrafish model of embryonic vitamin E (VitE) deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio), fed defined diets without (E-) or with VitE (E+, 500 mgRRR-α-tocopheryl acetate/kg diet), were spawned to obtain E- and E+ embryos that we evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf), neurobehavioral development (locomotor responses at 96 hpf), and rescue strategies. Rescues were attempted using micro-injection into the yolksac using VitE (as a phospholipid emulsion containing d6-α-tocopherol at 0 hpf) orD-glucose (in saline at 24 hpf).
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: McDougall
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Choi
authors.affiliation: The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hye-Kyeong
authors.initials: HK
authors.lastname: Kim
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Gerd
authors.initials: G
authors.lastname: Bobe
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: J Frederik
authors.initials: JF
authors.lastname: Stevens
authors.affiliation: University of Southern California, School of Pharmacy, Los Angeles, CA 90089, USA.
authors.forename: Enrique
authors.initials: E
authors.lastname: Cadenas
authors.affiliation: Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA; Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA; Sinnhuber Aquatic Research Laboratory, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Tanguay
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Maret G
authors.initials: MG
authors.lastname: Traber
keywords: Alpha-tocopherol; Choline; Docosahexaenoic acid; Ferroptosis; Glucose