title: Distinctive role of efferocytosis in dendritic cell maturation and migration in sterile or infectious conditions.
abstract: Efferocytosis, or clearance of apoptotic cells (ACs), by dendritic cells (DCs) leads to immune response suppression and tolerance to self-antigens. However, efferocytosis of infected apoptotic cells (IACs) leads to the production of a mixed pro- and anti-inflammatory cytokine milieu. We examined the DC phenotype and ability to migrate after phagocytosis of ACs or IACs and observed higher levels of CD86 and CCR7 expression in DCs, as well as enhanced migration capacity following efferocytosis of IACs. Interestingly, higher levels of interleukin-1β, interleukin-10 and prostaglandin E2(PGE2) were also produced in this context. Blockage of IAC recognition led to an impaired maturation profile and PGE2production, which may have contributed to reduced CD86 and CCR7 expression and migration capacity. These data contribute to the understanding of how efferocytosis of sterile or infected cells may regulate the adaptive immune response, although the precise role of PGE2in this process requires further investigation.
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
authors.forename: Letícia de Aquino
authors.initials: LA
authors.lastname: Penteado
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.|Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Naiara Naiana
authors.initials: NN
authors.lastname: Dejani
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.|Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Felipe Fortino
authors.initials: FF
authors.lastname: Verdan
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
authors.forename: Allan Botinhon
authors.initials: AB
authors.lastname: Orlando
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
authors.forename: Victoria Eugenia
authors.initials: VE
authors.lastname: Niño
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
authors.forename: Fernanda De Nuzzi
authors.initials: FN
authors.lastname: Dias
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.|Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Ana Carolina Guerta
authors.initials: ACG
authors.lastname: Salina
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
authors.forename: Alexandra Ivo
authors.identifier: 0000-0001-6048-3647
authors.initials: AI
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D017209:Apoptosis; D051940:B7-2 Antigen / Q000378:metabolism; D054415:Chemokine CCL19 / Q000378:metabolism; D054421:Chemokine CCL21 / Q000378:metabolism; D002633:Chemotaxis; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D015232:Dinoprostone / Q000378:metabolism; D004927:Escherichia coli Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010587:Phagocytosis; D010641:Phenotype; D000067996:RAW 264.7 Cells; D054400:Receptors, CCR7 / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051940:B7-2 Antigen; C515461:Ccl19 protein, mouse; C515371:Ccr7 protein, mouse; C497965:Cd86 protein, mouse; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D018836:Inflammation Mediators; D054400:Receptors, CCR7; D015232:Dinoprostone
keywords: CCR7; dendritic cells; efferocytosis; infected apoptotic cell; prostaglandin E2