title: Universal Super-Resolution Multiplexing by DNA Exchange.
abstract: Super-resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20× higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time-consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super-resolution techniques such as (d)STORM, STED, and SIM. We assay our approach using DNA origami nanostructures to quantitatively assess labeling, imaging, and washing efficiency. We furthermore demonstrate the applicability of our approach by imaging multiple protein targets in fixed cells.
authors.affiliation: Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.|Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Schueder
authors.affiliation: Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.|Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Maximilian T
authors.initials: MT
authors.lastname: Strauss
authors.affiliation: Department of Biology II and Center for Nanoscience, LMU Munich, Grosshaderner Strasse 2, 82152, Martinsried, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Hoerl
authors.affiliation: Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.|Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Schnitzbauer
authors.affiliation: Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.|Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schlichthaerle
authors.affiliation: Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.|Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Strauss
authors.affiliation: Wyss Institute for Biologically Inspired Engineering and Department of Systems Biology, Harvard University, 3 Blackfan Circle, Boston, MA, 02115, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Yin
authors.affiliation: Department of Biology II and Center for Nanoscience, LMU Munich, Grosshaderner Strasse 2, 82152, Martinsried, Germany.
authors.forename: Hartmann
authors.initials: H
authors.lastname: Harz
authors.affiliation: Department of Biology II and Center for Nanoscience, LMU Munich, Grosshaderner Strasse 2, 82152, Martinsried, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Leonhardt
authors.affiliation: Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.|Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Jungmann
mesh_terms: D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D061848:Optical Imaging
chemical_list: D015342:DNA Probes; D004247:DNA
keywords: DNA nanotechnology; SIM; STED; dSTORM; multiplexing