title: Protective effects of aloperine on monocrotaline-induced pulmonary hypertension in rats.
abstract: Pulmonary hypertension (PH) is serious, fatal disease which is promoted by oxidative stress. Aloperine have antioxidation effects, which effects on pulmonary arteries remain unclear. Therefore, this study is designed to investigate whether aloperine has protective effects on PH induced by monocrotaline and whether these effects are associated with oxidative stress. PH was induced by monocrotaline (60mg/kg), and subsequently oral administration of aloperine (25, 50, 100mg/kg/day). At the end of the experiment, hemodynamic, pathomorphologic, electrocardiographic and echocardiographic data from the rats were obtained. At same time, oxidative stress biomarkers (superoxide dismutase, malonyldialdehyde, catalase, glutathione peroxidase, total antioxidant capacity) and the protein expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX)-2, NOX-4 in the lung of rat has been detected. The result shows that aloperine treatment showed significantly improvement in hemodynamic, pathomorphologic, electrocardiographic and echocardiographic data. Moreover, aloperine treatment can alleviate the changes of oxidative stress biomarkers and suppress the expression levels of NOX-2, NOX-4. In summary, this study indicates that aloperine have protective effects on monocrotaline-induced PH. And these effects may be related to inhibit oxidative stress.
authors.affiliation: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Yinju
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Wanxia
authors.initials: W
authors.lastname: Yao
authors.affiliation: Echocardiogram Room, Heart Center, General Hospital of Ningxia Medical University, Yinchuan750004, PR China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.affiliation: Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, PR China; Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Yinchuan 750004, PR China. Electronic address: yujq910315@163.com.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, PR China; Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, PR China; Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Yinchuan 750004, PR China. Electronic address: zhou-ru926@163.com.
authors.forename: Ru
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004452:Echocardiography; D004562:Electrocardiography; D006976:Hypertension, Pulmonary / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000517:prevention & control*; D017380:Hypertrophy, Right Ventricular / Q000000981:diagnostic imaging / Q000517:prevention & control; D008297:Male; D016686:Monocrotaline; D018384:Oxidative Stress / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011651:Pulmonary Artery / Q000378:metabolism / Q000473:pathology; D011652:Pulmonary Circulation / Q000187:drug effects; D054837:Quinolizidines; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010880:Piperidines; D020011:Protective Agents; D054837:Quinolizidines; C062701:aloperine; D016686:Monocrotaline
keywords: Aloperine; Monocrotaline; NOX-2; NOX-4; Oxidative stress; Pulmonary hypertension