title: BRCA1-Associated Protein-1 Suppresses Osteosarcoma Cell Proliferation and Migration Through Regulation PI3K/Akt Pathway.
abstract: BRCA1-associated protein-1 (BAP1) is an important nuclear-localized deubiquitinating enzyme. Dysregulation of BAP1 has been reported in many types of cancers. However, there are few articles on the role of BAP1 in osteosarcoma (OS) and the molecular mechanisms of BAP1 in OS remain largely unknown. In this study, we examined the expression of BAP1 in the tissue sample from OS patients and healthy control subjects, and then investigated the biological function and molecular mechanisms of BAP1 in OS. We found that BAP1 was significantly reduced in OS patients and OS cell lines. Then we found that BAP1 has a key role in OS cell proliferation, apoptosis, migration, and invasion. Furthermore, we found that BAP1 exerted its influence on the PI3K/Akt signaling pathway and there was physical association between BAP1 and miR-125. In conclusion, our data highlight the important roles of BAP1 in the survival of OS. It may be a potential therapy for OS.
authors.affiliation: 1 First Department of Orthopedics, Cangzhou City Central Hospital , Cangzhou, People's Republic of China .
authors.forename: Shuming
authors.initials: S
authors.lastname: Gao
authors.affiliation: 1 First Department of Orthopedics, Cangzhou City Central Hospital , Cangzhou, People's Republic of China .
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Sun
authors.affiliation: 1 First Department of Orthopedics, Cangzhou City Central Hospital , Cangzhou, People's Republic of China .
authors.forename: Cai
authors.initials: C
authors.lastname: Cheng
authors.affiliation: 2 Second Department of Endocrinology and Metabolism, Cangzhou City Central Hospital , Cangzhou, People's Republic of China .
authors.forename: Guangya
authors.initials: G
authors.lastname: Wang
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000502:physiology*; D043222:Ubiquitin Thiolesterase / Q000502:physiology*
chemical_list: C400134:BAP1 protein, human; D025521:Tumor Suppressor Proteins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D043222:Ubiquitin Thiolesterase
keywords: BAP1; PI3K/Akt pathway; miR-125; osteosarcoma