title: Two-Way Chemical Communication between Artificial and Natural Cells.
abstract: Artificial cells capable of both sensing and sending chemical messages to bacteria have yet to be built. Here we show that artificial cells that are able to sense and synthesize quorum signaling molecules can chemically communicate withV. fischeri,V. harveyi,E. coli, andP. aeruginosa. Activity was assessed by fluorescence, luminescence, RT-qPCR, and RNA-seq. Two potential applications for this technology were demonstrated. First, the extent to which artificial cells could imitate natural cells was quantified by a type of cellular Turing test. Artificial cells capable of sensing and in response synthesizing and releasingN-3-(oxohexanoyl)homoserine lactone showed a high degree of likeness to naturalV. fischeriunder specific test conditions. Second, artificial cells that sensedV. fischeriand in response degraded a quorum signaling molecule ofP. aeruginosa(N-(3-oxododecanoyl)homoserine lactone) were constructed, laying the foundation for future technologies that control complex networks of natural cells.
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Lentini
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Noël Yeh
authors.initials: NY
authors.lastname: Martín
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Forlin
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Belmonte
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Jason
authors.initials: J
authors.lastname: Fontana
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Cornella
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Martini
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Tamburini
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland , College Park, Maryland 20742, United States.
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Olivier
authors.initials: O
authors.lastname: Jousson
authors.affiliation: CIBIO, University of Trento , via Sommarive 9, 38123 Povo, Italy.
authors.forename: Sheref S
authors.initials: SS
authors.lastname: Mansy