title: Gene Silencing of Selenoprotein K Induces Inflammatory Response and Activates Heat Shock Proteins Expression in Chicken Myoblasts.
abstract: In the present study, specific small interfering RNA (siRNA) for selenoprotein K (Selk) gene was designed and transfected into chicken myoblasts. Then, the expressions of inflammatory factors (including induced nitric oxide synthase [iNOS], nuclear factor-kappa B [NF-κB], heme-oxygenase-1 [HO-1], cyclooxygenase-2 [COX-2], and prostaglandin E synthase [PTGEs]), inflammation-related cytokines (including interleukin [IL]-1β, IL-6, IL-7, IL-8, IL-17, and interferon [IFN]-γ), and heat shock proteins (HSPs) (including HSP27, HSP40, HSP60, HSP70, and HSP90) were examined at 24 and 72 h after transfection. The results showed that messenger RNA (mRNA) expressions of iNOS, NF-κB, HO-1, COX-2, IL-6, IL-7, IL-8, HSP 27, HSP 40, HSP 60, HSP 70, and HSP 90 were significantly increased (p < 0.05) at 24 and 72 h after siRNA transfection, and the mRNA expressions of PTGEs, IL-1β, IL-17, and IFN-γ were significantly increased and decreased (p < 0.05) at 24 and 72 h after siRNA transfection. The results also showed that the protein expressions of iNOS, NF-κB, HO-1, COX-2, HSP60, HSP70, and HSP90 were significantly increased (p < 0.05) at 24 and 72 h after siRNA transfection. The correlation analysis and principal component analysis (PCA) showed that PTGEs, IL-1β, IL-17, IFN-γ, HSP40, and HSP90 might play special roles in response to Selk silencing in chicken myoblasts. These results indicated that Selk silencing induced inflammation response by affecting the expression levels of inflammatory factors and inflammation-related cytokines, and the heat shock proteins might play protective roles in this response in chicken myoblasts.
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Fan
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Cao
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Khalid
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. zhangziwe@sina.com.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. shiwenxu@neau.edu.cn.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D002642:Chick Embryo; D051546:Cyclooxygenase 2 / Q000235:genetics; D016207:Cytokines / Q000235:genetics; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006360:Heat-Shock Proteins / Q000235:genetics*; D051547:Heme Oxygenase-1 / Q000235:genetics; D007249:Inflammation / Q000235:genetics*; D032446:Myoblasts / Q000502:physiology*; D016328:NF-kappa B / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D025341:Principal Component Analysis; D034741:RNA, Small Interfering; D051140:Selenoproteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D016207:Cytokines; D006360:Heat-Shock Proteins; D016328:NF-kappa B; D034741:RNA, Small Interfering; D051140:Selenoproteins; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D051546:Cyclooxygenase 2
keywords: Chicken myoblast; Cytokine; Heat shock protein; Inflammatory factor; Selenoprotein K