title: Three-dimensional Organization of Layered Apical Cytoskeletal Networks Associated with Mouse Airway Tissue Development.
abstract: The cytoskeleton is an essential cellular component that enables various sophisticated functions of epithelial cells by forming specialized subcellular compartments. However, the functional and structural roles of cytoskeletons in subcellular compartmentalization are still not fully understood. Here we identified a novel network structure consisting of actin filaments, intermediate filaments, and microtubules directly beneath the apical membrane in mouse airway multiciliated cells and in cultured epithelial cells. Three-dimensional imaging by ultra-high voltage electron microscopy and immunofluorescence revealed that the morphological features of each network depended on the cell type and were spatiotemporally integrated in association with tissue development. Detailed analyses using Odf2 mutant mice, which lack ciliary basal feet and apical microtubules, suggested a novel contribution of the intermediate filaments to coordinated ciliary beating. These findings provide a new perspective for viewing epithelial cell differentiation and tissue morphogenesis through the structure and function of apical cytoskeletal networks.
authors.affiliation: Graduate School of Frontier Biosciences and Medicine, Osaka University, Osaka 5650871, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tateishi
authors.affiliation: Graduate School of Frontier Biosciences and Medicine, Osaka University, Osaka 5650871, Japan.|Japan Textile Products Quality and Technology Center, Kobe 6500011, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nishida
authors.affiliation: Research Center for Ultra-high Voltage Electron Microscopy, Osaka University, Osaka 5670047, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Inoue
authors.affiliation: Graduate School of Frontier Biosciences and Medicine, Osaka University, Osaka 5650871, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsukita
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002923:Cilia / Q000378:metabolism / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007382:Intermediate Filaments / Q000378:metabolism* / Q000648:ultrastructure; D061985:Madin Darby Canine Kidney Cells; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D009024:Morphogenesis; D014132:Trachea / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D006360:Heat-Shock Proteins; C490106:Odf2 protein, mouse