title: Production of the medaka derived from vitrified whole testes by germ cell transplantation.
abstract: The medaka (Oryzias latipes) is a teleost model distinguished from other model organisms by the presence of inbred strains, wild stocks, and related species. Cryopreservation guarantees preservation of these unique biological resources. However, because of their large size, cryopreservation techniques for their eggs and embryos have not been established. In the present study, we established a methodology to produce functional gametes from cryopreserved testicular cells (TCs). Whole testes taken from medaka were cryopreserved by vitrification. After thawing, the cells dissociated from cryopreserved testicular tissues were intraperitoneally transplanted into sterile triploid hatchlings. Some cells, presumably spermatogonial stem cells, migrated into the genital ridges of recipients and resulted in the production of eggs or sperm, based on sex of the recipient. Mating of recipients resulted in successful production of cryopreserved TC-derived offspring. We successfully produced individuals from the Kaga inbred line, an endangered wild population in Tokyo, and a sub-fertile mutant (wnt4b-/-) from cryopreserved their TCs. This methodology facilitates semi-permanent preservation of various medaka strains.
authors.affiliation: Bioscience Education and Research Support Center, Akita University, 1-1-1 Hondo, Akita, Akita 010-8543, Japan.|Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Seki
authors.affiliation: Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kusano
authors.affiliation: Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Seungki
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Iwasaki
authors.affiliation: Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Yagisawa
authors.affiliation: Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Ishida
authors.affiliation: Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hiratsuka
authors.affiliation: Laboratory of Bioresources, National Institute for Basic Biology, 38 Saigo-naka, Myodaiji-cho, Okazaki, Aichi 444-8585, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Sasado
authors.affiliation: Laboratory of Bioresources, National Institute for Basic Biology, 38 Saigo-naka, Myodaiji-cho, Okazaki, Aichi 444-8585, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Naruse
authors.affiliation: Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Yoshizaki
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D005854:Germ Cells / Q000502:physiology* / Q000528:radiation effects*; D008297:Male; D009990:Oryzias / Q000254:growth & development*; D013737:Testis / Q000166:cytology*; D058989:Vitrification