title: The Peroxiredoxin Family: An Unfolding Story.
abstract: Peroxiredoxins (Prxs) are a large and conserved family of peroxidases that are considered to be the primary cellular guardians against oxidative stress in all living organisms. Prxs share a thioredoxin fold and contain a highly-reactive peroxidatic cysteine in a specialised active-site environment that is able to reduce their peroxide substrates. The minimal functional unit for Prxs are either monomers or dimers, but many dimers assemble into decameric rings. Ring structures can further form a variety of high molecular weight complexes. Many eukaryotic Prxs contain a conserved GGLG and C-terminal YF motif that confer sensitivity to elevated levels of peroxide, leading to hyperoxidation and inactivation. Inactive forms of Prxs can be re-reduced by the enzyme sulfiredoxin, in an ATP-dependent reaction. Cycles of hyperoxidation and reactivation are considered to play an integral role in a variety of H2O2-mediated cell signalling pathways in both stress and non-stress conditions. Prxs are also considered to exhibit chaperone-like properties when cells are under oxidative or thermal stress. The roles of various types of covalent modifications, e.g. acetylation and phosphorylation are also discussed. The ability of Prxs to assemble into ordered arrays such as nanotubes is currently being exploited in nanotechnology.
authors.affiliation: Institute of Molecular, Cell and Systems Biology, Davidson Building, College of Medicine, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Institute of Molecular, Cell and Systems Biology, Davidson Building, College of Medicine, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK. Gordon.Lindsay@glasgow.ac.uk.
authors.forename: John Gordon
authors.initials: JG
authors.lastname: Lindsay
mesh_terms: D003545:Cysteine / Q000378:metabolism; D018869:Heat-Shock Response; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D003545:Cysteine
keywords: Antioxidation; Chaperone; Hyperoxidation; Oxidative stress; Peroxide; Peroxiredoxin; Protein complex; Redox; Signalling pathway; Sulfiredoxin