title: Gene Deletion in Barley Mediated by LTR-retrotransposon BARE.
abstract: A poly-row branched spike (prbs) barley mutant was obtained from soaking a two-rowed barley inflorescence in a solution of maize genomic DNA. Positional cloning and sequencing demonstrated that the prbs mutant resulted from a 28 kb deletion including the inflorescence architecture gene HvRA2. Sequence annotation revealed that the HvRA2 gene is flanked by two LTR (long terminal repeat) retrotransposons (BARE) sharing 89% sequence identity. A recombination between the integrase (IN) gene regions of the two BARE copies resulted in the formation of an intact BARE and loss of HvRA2. No maize DNA was detected in the recombination region although the flanking sequences of HvRA2 gene showed over 73% of sequence identity with repetitive sequences on 10 maize chromosomes. It is still unknown whether the interaction of retrotransposons between barley and maize has resulted in the recombination observed in the present study.
authors.affiliation: National Barley Improvement Centre, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Department of Genetics and Cell Biology, Yangtze University, Jingzhou, Hubei 434023, China.|Western Barley Genetics Alliance, Murdoch University, 90 South Street, Murdoch WA 6150, Australia.
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.affiliation: Luke/BI Plant Genomics Lab, Institute of Biotechnology and Viikki Plant Science Centre, University of Helsinki, FIN-00014 Helsinki, Finland.|Green Technology, Natural Resources Institute Finland (Luke), Viikinkaari 1, FIN-00790 Helsinki, Finland.
authors.forename: Alan H
authors.initials: AH
authors.lastname: Schulman
authors.affiliation: National Barley Improvement Centre, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Jinghuan
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Western Barley Genetics Alliance, Murdoch University, 90 South Street, Murdoch WA 6150, Australia.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: National Barley Improvement Centre, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Junmei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Western Barley Genetics Alliance, Murdoch University, 90 South Street, Murdoch WA 6150, Australia.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Jia
authors.affiliation: National Barley Improvement Centre, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.affiliation: National Barley Improvement Centre, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Genetics and Cell Biology, Yangtze University, Jingzhou, Hubei 434023, China.|Western Barley Genetics Alliance, Murdoch University, 90 South Street, Murdoch WA 6150, Australia.
authors.forename: Chengdao
authors.initials: C
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D017353:Gene Deletion; D017343:Genes, Plant / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D056626:Inflorescence / Q000235:genetics; D016254:Mutagenesis, Insertional; D010940:Plant Proteins / Q000235:genetics; D018626:Retroelements / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D020079:Terminal Repeat Sequences / Q000235:genetics*; D003313:Zea mays / Q000235:genetics
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D018626:Retroelements