title: Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury.
abstract: Wallerian degeneration (WD) in injured peripheral nerves is associated with a large number of up- or down-regulated genes, but the effects of these changes are poorly understood. In our previous studies, we reported some key factors that are differentially expressed to activate nerve degeneration and regeneration during WD. Here, we determined the effects of secreted phosphoprotein 1 (Spp1) on WD after rat sciatic nerve injury.
  
  
  
  Spp1 was upregulated from 6 h to 14 days after sciatic nerve injury. Altered expression of Spp1 in Schwann cells (SC) resulted in altered mRNA and protein expression levels for cytokines, c-Fos, PKCα and phospho-ERK/ERK and affected SC apoptosis in vitro. Silencing of Spp1 expression in SCs using siRNA technology reduced proliferation and promoted migration of SCs in vitro. By contrast, overexpression of Spp1 promoted proliferation and reduced migration in SCs in vitro. Differential expression of Spp1 after sciatic nerve injury in vivo altered the expression of cytokines, c-Fos, PKCα, and the p-ERK/ERK pathway.
  
  
  
  Spp1 is a key regulatory factor that affects nerve degeneration and regeneration through c-Fos, PKCα and p-ERK/ERK pathways after rat sciatic nerve injury. These results shed new light on the role of Spp1 in nerve degeneration and regeneration during WD.
authors.affiliation: China-Japan Union Hospital of Jilin University, 126 Xiantai Road, Changchun, 130033, Jilin, People's Republic of China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nnatong, 226001, Jiangsu, People's Republic of China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nnatong, 226001, Jiangsu, People's Republic of China.
authors.forename: Huaiqin
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nnatong, 226001, Jiangsu, People's Republic of China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nnatong, 226001, Jiangsu, People's Republic of China.
authors.forename: Meiyuan
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nnatong, 226001, Jiangsu, People's Republic of China.|Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: China-Japan Union Hospital of Jilin University, 126 Xiantai Road, Changchun, 130033, Jilin, People's Republic of China. sscui916@126.com.
authors.forename: Shusen
authors.initials: S
authors.lastname: Cui
authors.affiliation: School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nnatong, 226001, Jiangsu, People's Republic of China. yaodb@ntu.edu.cn.
authors.forename: Dengbing
authors.initials: D
authors.lastname: Yao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D055785:Gene Knockdown Techniques; D020935:MAP Kinase Signaling System; D008297:Male; D009416:Nerve Regeneration; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism* / Q000503:physiopathology; D015398:Signal Transduction; D015854:Up-Regulation; D014855:Wallerian Degeneration / Q000378:metabolism*
chemical_list: D016207:Cytokines; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C506897:Spp1 protein, rat; D053495:Osteopontin; D051571:Protein Kinase C-alpha
keywords: Nerve regeneration; Rat; Schwann cells; Sciatic nerve injury; Secreted phosphoprotein 1 (Spp1); Wallerian degeneration