title: In vitro cytotoxicity effect and antibacterial performance of human lung epithelial cells A549 activity of Zinc oxide doped TiO2nanocrystals: Investigation of bio-medical application by chemical method.
abstract: We report the synthesis of high quality ZnO doped TiO2nanocrystals by chemical method at room temperature (RT), it can cause serious oxidative stress and DNA damage to human lung epithelial cells (A549) lines. Our aim in this study, to reduce the cytotoxicity effect of ZnO doped TiO2nanocrystals are widely in biological fields. Several studies have been performed to understand the influence of ZnO doped titanium dioxide (TiO2-NPs) on cell function; however the effects of nanoparticle against to exposure on the cell membrane have been duly addressed fascinatingly so far. However, In this interaction, which may alter cell metabolism and integrity, it is one of the importance to understand the modifications of the cell membrane, mechanisms of pulmonary A549 cell lines nanoparticles were uptake and the molecular pathway during the initial cell responses are still unclear and much more investigative efforts are need to properly characterize the ZnO doped titanium dioxide nanoparticles were reported successfully. In particular of the epithelial cells, upon particles are exposed human pulmonary epithelial cells (A549) to various concentrations of composition, structure and morphology of the nanocrystals were analyzed by X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM). XRD assessed the crystal structure of the nanocrystals which identified peaks associated with (002), (100) and (101) planes of hexagonal wurtzite-type ZnO with lattice constants of a=b=3.249Å and c=5.219Å. The IR results showed high purity of products and indicated that the nanocrystals are made up of TiO and ZnO bonds. The Photoluminescence (PL) spectra are dominated by a strong narrow band edge emission tunable in the blue region of the visible spectra indicating a narrow size distribution of ZnO/TiO2nanocrystals which exhibits antibacterial activity over a broad range of bacterial species and in particular against Stre. Mut where it out competes four other commonly used E.coli, Pse. Aug, Stre. Mut and Salm. sps, well as testing four different appropriate concentration from the results showed a significant gain in viable cell numbers of all four bacteria species, with 5mM and 10mM being the most effective and 2mM being the worst, where it provided an effective improvement from the growth mechanism has been also proposed to the current interest of these nanocrystals will discussed in detail.
authors.affiliation: UNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, PO Box 392, Pretoria, South Africa; Nanosciences African Network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, PO Box 722, Somerset West, Western Cape Province, South Africa. Electronic address: kaviyarasuloyolacollege@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Kaviyarasu
authors.affiliation: Research and Development Center, Bharathiyar University, Coimbatore 641046, India.
authors.forename: N
authors.initials: N
authors.lastname: Geetha
authors.affiliation: PG Research & Department of Chemistry, Auxilium College (Autonomous), Vellore, India.
authors.forename: K
authors.initials: K
authors.lastname: Kanimozhi
authors.affiliation: Department of Chemistry, St. Xavier's College (Autonomous), Tirunelveli 627002, India; LIFE, Department of Chemistry, Loyola College (Autonomous), Chennai 600034, India.
authors.forename: C
authors.initials: C
authors.lastname: Maria Magdalane
authors.affiliation: Research and Development Center, Bharathiyar University, Coimbatore 641046, India; Department of Physics, SBM College of Engineering and Technology, Dindigul -624 005, India.
authors.forename: S
authors.initials: S
authors.lastname: Sivaranjani
authors.affiliation: Research and Development Center, Bharathiyar University, Coimbatore 641046, India; Department of Physics, Khadir Mohideen College, Adirampattinam 614601, India.
authors.forename: A
authors.initials: A
authors.lastname: Ayeshamariam
authors.affiliation: UNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, PO Box 392, Pretoria, South Africa; National Isotope Centre, GNS Science, PO Box 31312, Lower Hutt 5010, New Zealand.
authors.forename: J
authors.initials: J
authors.lastname: Kennedy
authors.affiliation: UNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, PO Box 392, Pretoria, South Africa; Nanosciences African Network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, PO Box 722, Somerset West, Western Cape Province, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Maaza
mesh_terms: D000072283:A549 Cells; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012475:Salmonella / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000187:drug effects; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; C009495:titanium dioxide; D014025:Titanium; D015034:Zinc Oxide
keywords: Antibacterial activity; Lung epithelial cells; Optical microscopy; Structural morphology; Surface structure