title: [RNA immunoprecipitation technique for Drosophila melanogaster S2 cells].
abstract: RNA-binding proteins play an important role in RNA metabolism, especially in mRNA biogenesis and subsequent expression patterns regulation. RNA immunoprecipitation (RIP) is a powerful tool for detecting protein-RNA associations. In this paper, we briefly cover the history of this method for analyzing RNA-protein interactions and reviewing a number of modifications of the RIP technique. We also present an adjusted RIP protocol that was modified for Drosophila S2 cell culture. The use of this protocol allows one to perform the efficient precipitation of RNA-protein complexes and harvest RNA in amounts that are sufficient for its downstream analysis.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.|k-z-m@mail.ru.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Kachaev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: R A
authors.initials: RA
authors.lastname: Gilmutdinov
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Kopytova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Zheludkevich
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: Y V
authors.initials: YV
authors.lastname: Shidlovskii
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Kurbidaeva
mesh_terms: D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D047468:Immunoprecipitation; D012313:RNA / Q000302:isolation & purification*; D016601:RNA-Binding Proteins / Q000302:isolation & purification*
chemical_list: D016601:RNA-Binding Proteins; D012313:RNA
keywords: Drosophila melanogaster; PABP; RNA immunoprecipitation; RNA-binding proteins; S2 cells