title: Cytotoxicity and genotoxicity effects of arsenic trioxide on SQ20B human laryngeal carcinoma cells.
abstract: This study investigates the cytotoxicity and the genotoxicity induced by arsenic trioxide As2O3in human laryngeal SQ20B carcinoma cell line. SQ20B cells were exposed to graded concentrations of arsenic trioxide (2 and 5μM) for 48h. Comet assay and γ-H2AX foci formation were used for measuring DNA damages, flow cytometry was used to identify cell cycle alterations and apoptosis, while cell morphology was visualized using transmission electron microscopy. The results show a dose-dependent induction of DNA damages and double strand breaks, alterations in cell cycle and morphologic alterations of cells. These results prove that As2O3is highly cytotoxic and genotoxic at the micromolar range ina human laryngeal carcinoma cell line.
authors.affiliation: Unit of Marine and Environmental Toxicology, UR 09-03, Sfax University, IPEIS, BP 1172, 3018 Sfax, Tunisia. Electronic address: fatimatrabelsi02@gmail.com.
authors.forename: Fatma
authors.initials: F
authors.lastname: Trabelsi
authors.affiliation: Unit of Marine and Environmental Toxicology, UR 09-03, Sfax University, IPEIS, BP 1172, 3018 Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Khlifi
authors.affiliation: Normandie Univ, UNICAEN, CMABio, SFR ICORE, 14000 Caen, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Goux
authors.affiliation: Normandie Univ, UNICAEN, CMABio, SFR ICORE, 14000 Caen, France; Normandie Univ, UNICAEN, INSERM, COMETE, 14000 Caen, France.
authors.forename: Marilyne
authors.initials: M
authors.lastname: Guillamin
authors.affiliation: Unit of Marine and Environmental Toxicology, UR 09-03, Sfax University, IPEIS, BP 1172, 3018 Sfax, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Hamza-Chaffai
authors.affiliation: Normandie Univ, UNICAEN, ABTE, 14000 Caen, France; Centre François Baclesse, Avenue Général Harris, BP5026, F-14076 Caen Cedex-05, France.
authors.forename: François
authors.initials: F
authors.lastname: Sichel
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D020552:Comet Assay; D004249:DNA Damage; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D010087:Oxides / Q000494:pharmacology*; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide
keywords: Apoptosis; Arsenic trioxide; DNA damage; DNA double-strand breaks; Head and neck carcinoma cells; γH2AX