title: Microwave radiation is effective at disinfecting dental stone surfaces without changing their physical properties.
abstract: The aims of this study were to evaluate the effectiveness of different microwave radiation regimens for disinfection of type IV dental stone surfaces and to assess the influence of these regimens on surface roughness and dimensional change following disinfection. Three hundred cylindrical (20 × 2-mm) test specimens were made in type IV stone and divided into subgroups of 20 according to the microorganisms tested (Staphylococcus aureus, Escherichia coli, or Candida albicans) and the 900-W microwave radiation protocol (cycles of 3, 5, or 7 minutes; a positive control; or a negative control). To test physical changes, 80 test specimens were made with the same dimensions except that they had 2 parallel and symmetrical indentations measuring 8 × 4 mm. These specimens were divided into 4 subgroups of 20 each (a subgroup for each radiation time and a negative control). The mean dimensional change and roughness data were analyzed by mixed models for repeated measures and Tukey-Kramer tests. Disinfection was analyzed with descriptive statistics. For E coli and C albicans, all radiation times proved effective at sterilizing the test specimens. For S aureus, sterilization was achieved with 5 and 7 minutes of exposure; however, colonies were observed in 10 Petri dishes (50%) exposed to 3 minutes of microwave radiation. No statistically significant difference in dimensional change or surface roughness was observed for any radiation regimen (P > 0.05).
authors.forename: Ariel José
authors.initials: AJ
authors.lastname: Bona
authors.forename: Mauro Gustavo
authors.initials: MG
authors.lastname: Amaral-Brito
authors.forename: José Augusto
authors.initials: JA
authors.lastname: Rodrigues
authors.forename: Daiane Cristina
authors.initials: DC
authors.lastname: Peruzzo
authors.forename: Fabiana Mantovani Gomes
authors.initials: FM
authors.lastname: França
mesh_terms: D002176:Candida albicans / Q000528:radiation effects; D003760:Dental Impression Materials; D004203:Disinfection / Q000379:methods*; D004926:Escherichia coli / Q000528:radiation effects; D006801:Humans; D008872:Microwaves / Q000627:therapeutic use*; D013211:Staphylococcus aureus / Q000528:radiation effects; D013499:Surface Properties / Q000528:radiation effects
chemical_list: D003760:Dental Impression Materials
keywords: biological contamination; occupational hazards; prosthodontics