title: On-off affinity binding modulation on thermoresponsive polymer-grafted surfaces for capture and release of proteins and cells.
abstract: This review describes new types of thermoresponsive surfaces modified with biologically active ligands to thermally modulate on-off affinity binding between ligands and receptors. To achieve the thermal regulation of affinity binding, molecules of thermoresponsive polymer poly(N-isopropylacrylamide) (PIPAAm) are typically used to tune the degree of steric hindrance in response to temperature. The coil-to-globule transition of surface-grafted PIPAAm chains induces dynamic changes in the degree of steric hindrance in the vicinity of the immobilized ligand, resulting in the thermoresponsive modulation of on-off affinity interactions with receptors, and the subsequent on-off switching of the capture and release of proteins and cells on the surface. Thermoresponsive biomaterial surfaces to modulate on-off affinity binding between ligands and receptors has potential to realize the creation of functional cell sheet-based tissues, as well as novel separation system of specific proteins and cells without any labeling. These materials will thus be beneficial for clinical use in cell-based therapies, including tissue engineering and regenerative medicine.
authors.affiliation: a Institute of Advanced Biomedical Engineering and Science , Tokyo Women's Medical University (TWIns) , Tokyo , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.affiliation: a Institute of Advanced Biomedical Engineering and Science , Tokyo Women's Medical University (TWIns) , Tokyo , Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.affiliation: a Institute of Advanced Biomedical Engineering and Science , Tokyo Women's Medical University (TWIns) , Tokyo , Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D018914:Cells, Immobilized / Q000166:cytology*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013499:Surface Properties; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D055767:Immobilized Proteins; C052970:poly-N-isopropylacrylamide
keywords: Thermoresponsive polymer; affinity interaction; chromatography; poly(N-isopropylacrylamide); tissue engineering