title: High-sucrose diet induces diabetic-like phenotypes and oxidative stress in Drosophila melanogaster: Protective role of Syzygium cumini and Bauhinia forficata.
abstract: Diet is a key component for development and longevity of organisms. Here, the fruit fly was used to evaluate the detrimental effects caused by consumption of high-sucrose diets (HSD), namely phenotypic responses linked to insulin signaling and oxidative stress. The protective effects of extracts from medicinal plants Syzygium cumini and Bauhinia forficata were investigated. HSD intake (15% and 30%) delayed the time to pupation and reduced the number of white pupae. In adult flies, the intake of diets was associated with mortality and increased levels of glucose+trehalose, triacylglycerols and hydrogen peroxide. Indeed, 30% HSD induced body-weight loss, mitochondrial dysfunction and changes in acetylcholinesterase, δ-aminolevulinate dehydratase and antioxidant enzymes activity. Catalase, superoxide dismutase, keap1, HSP70, dILP-5 and Insulin receptor mRNA levels were over-expressed in flies emerged from 30% HSD. The extract treatments blunted the developmental alterations elicited by diets. Syzygium cumini extract was more efficient than B. forficata in reducing hyperglycaemia, redox disturbances and the changes in mRNA expression of insulin receptor.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Assis
authors.initials: A
authors.lastname: Ecker
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Thallita Karla Silva do Nascimento
authors.initials: TKSDN
authors.lastname: Gonzaga
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Rodrigo Lopes
authors.initials: RL
authors.lastname: Seeger
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Matheus Mulling Dos
authors.initials: MMD
authors.lastname: Santos
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Julia Sepel
authors.initials: JS
authors.lastname: Loreto
authors.affiliation: Departamento de Farmácia Industrial, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Daiane Francine
authors.initials: DF
authors.lastname: Meinerz
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Thiago Henrique
authors.initials: TH
authors.lastname: Lugokenski
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: João Batista Teixeira da
authors.initials: JBTD
authors.lastname: Rocha
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil. Electronic address: nvbarbosa@yahoo.com.br.
authors.forename: Nilda Vargas
authors.initials: NV
authors.lastname: Barbosa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D036481:Bauhinia / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D050260:Carbohydrate Metabolism / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D004032:Diet; D019422:Dietary Sucrose / Q000009:adverse effects*; D004331:Drosophila melanogaster; D006861:Hydrogen Peroxide / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007328:Insulin / Q000378:metabolism / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D011972:Receptor, Insulin / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D019422:Dietary Sucrose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D011972:Receptor, Insulin
keywords: Bauhinia forficata; Drosophila melanogaster; High-sugar diet; Oxidative stress; Sucrose; Syzygium cumini