title: Platelet microparticles: a biomarker for recanalization in rtPA-treated ischemic stroke patients.
abstract: OBJECTIVE
  Identification of a biomarker for acute recanalization could have significant clinical impact.
  
  
  METHODS
  We prospectively collected baseline, 24-h, and 90-day clinical and imaging data from consecutive ischemic stroke patients who fulfilled standard clinical eligibility criteria for treatment with intravenous recombinant tissue plasminogen activator (rtPA). Computed tomography angiography was acquired at 24 h and assessed using the thrombolysis is myocardial infarction (TIMI) scale with a score of 2b/3 indicating recanalization. Blood samples collected at 24 h after stroke symptom onset were used to measure the inflammatory biomarkers of glycoprotein IIb (CD41) expressing microparticles (MP), C-reactive protein (CRP), COX 2, APOE, and Angiopoietin 1. Analysis was performed using linear regression and Pearson's correlation coefficient.
  
  
  RESULTS
  A total of 57 patients met study eligibility criteria and had sufficient data and sample quality to be analyzed. Circulating levels of platelet derived CD41 + MP were significantly related to reperfusion (Pearson correlation, PC: 0.554,P< 0.001) and recanalization (PC: 0.495,P< 0.001) as well as related with 3-month modified Rankin Score (PC: 0.483,P< 0.001). CRP was significantly negatively correlated with recanalization on 24 h CTA (PC: -0.292,P= 0.041). Backward logistic regression with CRP and CD41 + MP increased the association with reperfusion (r2= 0.357P< 0.001).
  
  
  CONCLUSIONS
  There is a significant relationship between the inflammatory biomarkers CD41 + MP and CRP and recanalization.
authors.affiliation: Departments of Neurology John Hunter Hospital University of Newcastle Newcastle Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bivard
authors.affiliation: Hunter Haematology Research Group Calvary Mater Newcastle hospital Waratah Australia.
authors.forename: Lisa F
authors.initials: LF
authors.lastname: Lincz
authors.affiliation: Departments of Neurology John Hunter Hospital University of Newcastle Newcastle Australia.
authors.forename: Jane
authors.initials: J
authors.lastname: Maquire
authors.affiliation: Departments of Neurology John Hunter Hospital University of Newcastle Newcastle Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Parsons
authors.affiliation: Departments of Neurology John Hunter Hospital University of Newcastle Newcastle Australia.
authors.forename: Christopher
authors.initials: C
authors.lastname: Levi