title: Disinfection of Multidrug Resistant Escherichia coli by Solar-Photocatalysis using Fe-doped ZnO Nanoparticles.
abstract: Spread of antibiotic resistant bacteria through water, is a threat to global public health. Here, we report Fe-doped ZnO nanoparticles (Fe/ZnO NPs) based solar-photocatalytic disinfection (PCD) of multidrug resistant Escherichia coli (MDR E. coli). Fe/ZnO NPs were synthesized by chemical precipitation technique, and when used as photocatalyst for disinfection, proved to be more effective (time for complete disinfection = 90 min) than ZnO (150 min) and TiO2(180 min). Lipid peroxidation and potassium (K+) ion leakage studies indicated compromisation of bacterial cell membrane and electron microscopy and live-dead staining confirmed the detrimental effects on membrane integrity. Investigations indicated that H2O2was the key species involved in solar-PCD of MDR E. coli by Fe/ZnO NPs. X-ray diffraction and atomic absorption spectroscopy studies showed that the Fe/ZnO NPs system remained stable during the photocatalytic process. The Fe/ZnO NPs based solar-PCD process proved successful in the disinfection of MDR E. coli in real water samples collected from river, pond and municipal tap. The Fe/ZnO NPs catalyst made from low cost materials and with high efficacy under solar light may have potential for real world applications, to help reduce the spread of resistant bacteria.
authors.affiliation: School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT University), Bhubaneswar, 751024, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Das
authors.affiliation: School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT University), Bhubaneswar, 751024, India.
authors.forename: Sayantan
authors.initials: S
authors.lastname: Sinha
authors.affiliation: Department of Life Sciences, National Institute of Technology, Rourkela, 6150, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Das
authors.affiliation: Department of Life Sciences, National Institute of Technology, Rourkela, 6150, India.
authors.forename: R
authors.initials: R
authors.lastname: Jayabalan
authors.affiliation: School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT University), Bhubaneswar, 751024, India.
authors.forename: Mrutyunjay
authors.initials: M
authors.lastname: Suar
authors.affiliation: School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT University), Bhubaneswar, 751024, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Mishra
authors.affiliation: School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT University), Bhubaneswar, 751024, India.|Department of Public Health Sciences, Karolinska Institutet, SE, 17177, Stockholm, Sweden.
authors.forename: Ashok J
authors.initials: AJ
authors.lastname: Tamhankar
authors.affiliation: Department of Public Health Sciences, Karolinska Institutet, SE, 17177, Stockholm, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Stålsby Lundborg
authors.affiliation: School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT University), Bhubaneswar, 751024, India. tripathy.suraj@gmail.com.|School of Applied Sciences, KIIT University, Bhubaneswar, 751024, India. tripathy.suraj@gmail.com.|Center of Industrial Technology, KIIT University, Bhubaneswar, 751024, India. tripathy.suraj@gmail.com.
authors.forename: Suraj K
authors.initials: SK
authors.lastname: Tripathy
mesh_terms: D002384:Catalysis; D018360:Crystallography, X-Ray; D004203:Disinfection / Q000379:methods*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D007501:Iron / Q000737:chemistry / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry; D011188:Potassium / Q000378:metabolism; D013472:Sunlight; D014871:Water Microbiology; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D007501:Iron; D011188:Potassium; D015034:Zinc Oxide