title: Comparative Analysis of Oxidative Stress During Aging of Kluyveromyces marxianus in Synthetic and Whey Media.
abstract: During the aging of yeast culture, Kluyveromyces marxianus undergoes a number of changes in physiology and these changes play a significant role during fermentation. Aged stationary phase cells were found to contain more reactive oxygen species. Additionally, the level of oxidant is counteracted by the antioxidant defense system of the cells. Comparison of 3-day-old culture of K. marxianus with 45-day stationary phase culture represents an increased level of ROS inside the cells. Moreover, a decrease in glutathione content was observed over the set of the incubation period. The increased level of superoxide dismutase (SOD) and catalase also revealed that there is oxidative stress during the long period incubation of the stationary phase cells of K. marxianus. The actual phenomenon of aging in dairy yeast K. marxianus is a complex process, but the present study signifies that role of antioxidant defense system during aging in stationary phase cells of K. marxianus.
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Saini
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Beniwal
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India. shilpavijn@yahoo.co.in.
authors.forename: Shilpa
authors.identifier: http://orcid.org/0000-0003-0518-7727
authors.initials: S
authors.lastname: Vij
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D005656:Fungal Proteins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007716:Kluyveromyces / Q000378:metabolism*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000067796:Whey / Q000737:chemistry*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Catalase; Free radicals; Glutathione; Kluyveromyces marxianus; Oxidative stress