title: Phycobilisome truncation causes widespread proteome changes in Synechocystis sp. PCC 6803.
abstract: In cyanobacteria such as Synechocystis sp. PCC 6803, large antenna complexes called phycobilisomes (PBS) harvest light and transfer the energy to the photosynthetic reaction centers. Modification of the light harvesting machinery in cyanobacteria has widespread consequences, causing changes in cell morphology and physiology. In the current study, we investigated the effects of PBS truncation on the proteomes of three Synechocystis 6803 PBS antenna mutants. These range from the progressive truncation of phycocyanin rods in the CB and CK strains, to full removal of PBS in the PAL mutant. Comparative quantitative protein results revealed surprising changes in protein abundances in the mutant strains. Our results showed that PBS truncation in Synechocystis 6803 broadly impacted core cellular mechanisms beyond light harvesting and photosynthesis. Specifically, we observed dramatic alterations in membrane transport mechanisms, where the most severe PBS truncation in the PAL strain appeared to suppress the cellular utilization and regulation of bicarbonate and iron. These changes point to the role of PBS as a component critical to cell function, and demonstrate the continuing need to assess systems-wide protein based abundances to understand potential indirect phenotypic effects.
authors.affiliation: Department of Biology, Washington University, St. Louis, Missouri, United States of America.
authors.forename: Michelle
authors.initials: M
authors.lastname: Liberton
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: William B
authors.initials: WB
authors.lastname: Chrisler
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: Carrie D
authors.initials: CD
authors.lastname: Nicora
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: David W
authors.initials: DW
authors.lastname: Koppenaal
authors.affiliation: Department of Biology, Washington University, St. Louis, Missouri, United States of America.
authors.forename: Himadri B
authors.initials: HB
authors.lastname: Pakrasi
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America.
authors.forename: Jon M
authors.initials: JM
authors.lastname: Jacobs
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D045524:Phycobilisomes / Q000378:metabolism*; D020543:Proteome; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D045524:Phycobilisomes; D020543:Proteome; D007501:Iron; D009584:Nitrogen