title: The modified base isopentenyladenosine and its derivatives in tRNA.
abstract: Base 37 in tRNA, 3'-adjacent to the anticodon, is occupied by a purine base that is thought to stabilize codon recognition by stacking interactions on the first Watson-Crick base pair. If the first codon position forms an A.U or U.A base pair, the purine is likely further modified in all domains of life. One of the first base modifications found in tRNA is N6-isopentenyl adenosine (i6A) present in a fraction of tRNAs in bacteria and eukaryotes, which can be further modified to 2-methyl-thio-N6-isopentenyladenosine (ms2i6A) in a subset of tRNAs. Homologous tRNA isopentenyl transferase enzymes have been identified in bacteria (MiaA), yeast (Mod5, Tit1), roundworm (GRO-1), and mammals (TRIT1). In eukaryotes, isopentenylation of cytoplasmic and mitochondrial tRNAs is mediated by products of the same gene. Accordingly, a patient with homozygous mutations in TRIT1 has mitochondrial disease. The role of i6A in a subset of tRNAs in gene expression has been linked with translational fidelity, speed of translation, skewed gene expression, and non-sense suppression. This review will not cover the action of i6A as a cytokinin in plants or the potential function of Mod5 as a prion in yeast.
authors.affiliation: a Institut für Biochemie und Molekularbiologie, Rheinische Friedrich-Wilhelms-Universität Bonn , Bonn , Germany.
authors.forename: Ulrich
authors.identifier: 0000-0003-1380-4780
authors.initials: U
authors.lastname: Schweizer
authors.affiliation: a Institut für Biochemie und Molekularbiologie, Rheinische Friedrich-Wilhelms-Universität Bonn , Bonn , Germany.
authors.forename: Simon
authors.identifier: 0000-0002-1551-9839
authors.initials: S
authors.lastname: Bohleber
authors.affiliation: a Institut für Biochemie und Molekularbiologie, Rheinische Friedrich-Wilhelms-Universität Bonn , Bonn , Germany.
authors.forename: Noelia
authors.identifier: 0000-0003-3418-0743
authors.initials: N
authors.lastname: Fradejas-Villar
mesh_terms: D000818:Animals; D000926:Anticodon; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D003062:Codon; D004198:Disease Susceptibility; D006801:Humans; D007541:Isopentenyladenosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D008745:Methylation; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D011687:Purines / Q000737:chemistry / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D000926:Anticodon; D003062:Codon; D011687:Purines; D007541:Isopentenyladenosine; D012343:RNA, Transfer
keywords: TRIT1; i6A; miaA; mitochondria; mod5; modification; selenoprotein; tRNA