title: Box-Behnken analysis and storage of spray-dried collagenolytic proteases from Myceliophthora thermophila submerged bioprocess.
abstract: Enzymes do not have long-term storage stability in soluble forms, thus drying methods could minimize the loss of enzymatic activity, the spray dryer removes water under high temperatures and little time. The aims of this study were to improve the stability of enzymatic extract from Myceliophthora thermophila for potential applications in industry and to evaluate the best conditions to remove the water by spray drying technique. The parameters were tested according to Box-Behnken and evaluated by analysis of variance (ANOVA), all the parameters measured were found to influence the final enzyme activity and spray drying process yield ranged from 38.65 to 63.75%. Enzyme powders showed increased storage stability than extract and maintained about 100% of collagenolytic activity after 180 days of storage at 30°C. The results showed that the microbial enzymes maintained activity during the spray drying process and were stable during long-term storage; these are promising characteristics for industrial applications.
authors.affiliation: a Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Youssef Ali Abou
authors.initials: YAA
authors.lastname: Hamin Neto
authors.affiliation: a Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Luciana Barbosa
authors.initials: LB
authors.lastname: Coitinho
authors.affiliation: a Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Luis Alexandre Pedro
authors.initials: LAP
authors.lastname: de Freitas
authors.affiliation: a Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Hamilton
authors.initials: H
authors.lastname: Cabral
mesh_terms: D000704:Analysis of Variance; D003094:Collagen / Q000378:metabolism; D003890:Desiccation; D004795:Enzyme Stability; D007218:Industrial Microbiology; D010447:Peptide Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D059748:Proteolysis; D020037:Sordariales / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
chemical_list: D003094:Collagen; D010447:Peptide Hydrolases
keywords: Box–Behnken; Myceliophthora thermophila; collagenolytic protease; filamentous fungi; multivariate analysis; spray dryer