title: Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae-Arabidopsis thaliana Interaction.
abstract: The symptoms of the clubroot disease on Brassica species caused by the obligate biotrophic protist Plasmodiophora brassicae relies, among other factors, on the modulation of plant hormones. Signaling, transport as well as biosynthesis and metabolism are key features how the levels of auxins and cytokinins are controlled. We here describe (a) how to inoculate the model plant Arabidopsis thaliana with P. brassicae, (b) qualitative and quantitative methods to evaluate disease severity in auxin and cytokinin mutants,
authors.affiliation: Institut für Botanik, Technische Universität Dresden, Zellescher Weg 20b, 01062, Dresden, Germany. Jutta.Ludwig-Mueller@tu-dresden.de.
authors.forename: Jutta
authors.initials: J
authors.lastname: Ludwig-Müller
authors.affiliation: Institut für Botanik, Technische Universität Dresden, Zellescher Weg 20b, 01062, Dresden, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Auer
authors.affiliation: Institut für Botanik, Technische Universität Dresden, Zellescher Weg 20b, 01062, Dresden, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Jülke
authors.affiliation: Institut für Botanik, Technische Universität Dresden, Zellescher Weg 20b, 01062, Dresden, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Marschollek
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology*; D003583:Cytokinins / Q000378:metabolism*; D006790:Host-Parasite Interactions; D007210:Indoleacetic Acids / Q000378:metabolism*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010937:Plant Growth Regulators / Q000378:metabolism*; D056909:Plasmodiophorida / Q000502:physiology
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators
keywords: Arabidopsis thaliana; Auxin; Clubroot; Cytokinins; Hormone responsive promoter; Plasmodiophora brassicae; β-Glucuronidase