title: Injectable conductive collagen/alginate/polypyrrole hydrogels as a biocompatible system for biomedical applications.
abstract: Recently, Injectable Conducting Hydrogel (ICH) systems have gained much attention for tissue engineering and regenerative medicine. These systems can promote the regeneration of tissues responding to electrical responses. In this study, a novel ICH system was introduced. To achieve this system, firstly, a soluble non-toxic polypyrrole (PPy) synthesized by grafting pyrrole on alginate (Alg) backbone (Alg-graft-PPy), and then, ICH systems were prepared by the given ratios of Alg-graft-PPy, Alg, and collagen (Col). Three different amounts of Col (0.5, 1, and 1.5 mg/ml) were added to the system including Alg-graft-PPy: Alg wt. % with the ratios of 20:80 and 30:70. FTIR spectroscopy, electrical conductivity, viscosity, syringeability, gelation time, and MTT assay were performed in order to characterize the produced hydrogels. Due to the rheological behavior of 20:80 (Alg-graft-PPy: Alg wt. %), it was recognized more suitable to inject. Also this system associated with 0.5 mg/ml Col introduced as the best sample with respect to its viscosity and injectability. This ICH system has shown high conductivity in addition to a good level of cell viability and syringeability. With respect to properties of the produced ICH system, it can be applied for bone, nerve, muscle and cardiac cells, which respond to electrical impulses.
authors.affiliation: a Department of Biomedical Engineering , Amirkabir University of Technology (Tehran Polytechnic) , Tehran , Iran.
authors.forename: Farinaz
authors.initials: F
authors.lastname: Ketabat
authors.affiliation: a Department of Biomedical Engineering , Amirkabir University of Technology (Tehran Polytechnic) , Tehran , Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Karkhaneh
authors.affiliation: b Biomedical Engineering Faculty , Islamic Azad University, Science and Research Branch , Tehran , Iran.|c Tehran Heart Hospital Research Center, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Mehdinavaz Aghdam
authors.affiliation: c Tehran Heart Hospital Research Center, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Seyed
authors.initials: S
authors.lastname: Hossein Ahmadi Tafti
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry*; D004553:Electric Conductivity; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D008422:Materials Testing; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020100:Hydrogels; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D020723:Glucuronic Acid; D003094:Collagen
keywords: Injectable Conducting Hydrogel (ICH); alginate; collagen; polypyrrole