title: Protective effects of intraperitoneal administration of nimodipine on ischemia-reperfusion injury in ovaries: Histological and biochemical assessments in a rat model.
abstract: OBJECTIVE
  Ovarian torsion must be diagnosed and treated as much early as possible. The aim of the present study was to investigate effects of intraperitoneal administration of nimodipine on ischemia-reperfusion injury in ovaries.
  
  
  METHODS
  Thirty healthy male Wistar rats weighing approximately 250g were randomized into six experimental groups (n=5): Group Sham: The rats underwent only laparotomy. Group I: A 3-h ischemia only. Group I/R: A 3-h ischemia and a 3-h reperfusion. Group I/Nimodipine: A 3-h ischemia only and 1mg/kg intraperitoneal administration of nimodipine 2.5h after induction of ischemia. Group I/R/Nimodipine: A 3-h ischemia, a 3-h reperfusion and 1mg/kg intraperitoneal administration of nimodipine 2.5h after induction of ischemia.
  
  
  RESULTS
  Nimodipine treated animals showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (P<0.05). The significant higher values of SOD, tGSH, GPO, GSHRd and GST were observed in I/R/Nimodipine animals compared to those of other groups (P<0.05). The damage indicators (NOS, MDA, MPO and DNA damage level) were significantly lower in I/R/Nimodipine animal compared to those of other groups (P<0.05).
  
  
  CONCLUSIONS
  Intraperitoneal administration of nimodipine could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.
authors.affiliation: Reproductive Health Research Center, Department of Infertility, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Behroozi-Lak
authors.affiliation: Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: leilazarei652@yahoo.com.
authors.forename: Leila
authors.initials: L
authors.lastname: Zarei
authors.affiliation: Department of Nursing and Midwifery, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Mones
authors.initials: M
authors.lastname: Moloody-Tapeh
authors.affiliation: Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Negin
authors.initials: N
authors.lastname: Farhad
authors.affiliation: Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000008:administration & dosage* / Q000627:therapeutic use; D005260:Female; D007274:Injections, Intraperitoneal; D009553:Nimodipine / Q000008:administration & dosage* / Q000627:therapeutic use; D010049:Ovarian Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D002121:Calcium Channel Blockers; D009553:Nimodipine
keywords: Biochemical; Histopathology; Intraperitoneal; Ischemia–reperfusion; Nimodipine; Ovary; Rat