title: Immuno-PCR: Achievements and Perspectives.
abstract: The immuno-PCR (iPCR) method combines advantages of enzyme-linked immunosorbent assay and polymerase chain reaction, which is used in iPCR as a method of "visualization" of antigen-antibody interaction. The use of iPCR provides classical PCR sensitivity to objects traditionally detected by ELISA. This method could be very sensitive and allow for detection of quantities of femtograms/ml order. However, iPCR is still not widely used. The aim of this review is to highlight the special features of the iPCR method and to show the main aspects of its development and application in recent years.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. d.yu.ryazantsev@gmail.com.
authors.forename: D Y
authors.initials: DY
authors.lastname: Ryazantsev
authors.forename: D V
authors.initials: DV
authors.lastname: Voronina
authors.forename: S K
authors.initials: SK
authors.lastname: Zavriev
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D029322:Norovirus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D014118:Toxins, Biological / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D014118:Toxins, Biological; D004247:DNA