title: Therapeutic effects of different doses of polyethylene glycosylated porcine glucagon-like peptide-2 on ulcerative colitis in male rats.
abstract: BACKGROUND
  Polyethylene glycosylated (PEGylated) porcine glucagon-like peptide-2 (pGLP-2) considerably increases half-life and stability compared with the native pGLP-2, but the effective dose for intestinal damage is still unclear. This study aims to evaluate the available dose of polyethylene glycosylated porcine glucagon-like peptide-2 (PEG-pGLP-2), a modified, long-acting form of pGLP-2 in an experimental rat model of ulcerative colitis.
  
  
  METHODS
  Thirty-five male rats were randomly assigned into five groups: control, dextran sodium sulphate (DSS), DSS + PEG-pGLP-2(L), DSS + PEG-pGLP-2(M) and DSS + PEG-pGLP-2(H). Rats in control group received only water; other rats were fed with 5% (w/v) DSS and intraperitoneally administered with 12.5, 25 and 100 nmol/kg PEG-pGLP-2 daily for 6 days.
  
  
  RESULTS
  Compared with the control treatment, DSS treatment significantly (p < 0.05) decreased body weight change, colonic length, duodenal villus height and expression of zonula occludens-1, whereas significantly (p < 0.05) increased colonic damage score and expression of claudin-1, interleukin (IL)-1, IL-7, IL-10, interferon-γ and tumour necrosis factor (TNF)-α in colon. However, the three doses of PEG-pGLP-2 all reduced these effects; these treatments significantly (p < 0.05) increased body weight change and duodenal villus height, whereas significantly (p < 0.05) decreased colonic damage score and expression of IL-1, IL-7 and TNF-α in colon. Specifically, low-dose (12.5 nmol/kg/d) PEG-pGLP-2 was effective.
  
  
  CONCLUSIONS
  These results indicated that PEG-pGLP-2 is a novel and potentially effective therapy for intestinal healing in a relatively low dose.
authors.affiliation: Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Jianggan, Hangzhou, 310021, China.
authors.forename: Ke-Ke
authors.initials: KK
authors.lastname: Qi
authors.affiliation: Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Jianggan, Hangzhou, 310021, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Lv
authors.affiliation: Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Jianggan, Hangzhou, 310021, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Jianggan, Hangzhou, 310021, China. zjsnkyxzw@163.com.
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Xu
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D062445:Claudin-1 / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects*; D016207:Cytokines / Q000187:drug effects; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000187:drug effects; D053767:Glucagon-Like Peptide 2 / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D051381:Rats; D062826:Zonula Occludens-1 Protein / Q000187:drug effects
chemical_list: D062445:Claudin-1; D016207:Cytokines; D053767:Glucagon-Like Peptide 2; D062826:Zonula Occludens-1 Protein; D011092:Polyethylene Glycols; D016264:Dextran Sulfate
keywords: Inflammation cytokines; Porcine glucagon-like peptide-2; Tight junction; Ulcerative colitis