title: Cbx3 maintains lineage specificity during neural differentiation.
abstract: Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage specification by enhancing the expression of neural genes and down-regulating genes specific to alternative fates.
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.|Department of Neurobiology, Shantou University Medical College, Shantou 515041, China.
authors.forename: Chengyang
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Trent
authors.initials: T
authors.lastname: Su
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Fides D
authors.initials: FD
authors.lastname: Lay
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Robin A
authors.initials: RA
authors.lastname: McKee
authors.affiliation: Department of Neurobiology, Shantou University Medical College, Shantou 515041, China.
authors.forename: Meiyang
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Ajay
authors.initials: A
authors.lastname: Vashisht
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: James
authors.initials: J
authors.lastname: Wohlschlegel
authors.affiliation: Department of Neurobiology, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Bennett G
authors.initials: BG
authors.lastname: Novitch
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Plath
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Siavash K
authors.initials: SK
authors.lastname: Kurdistani
authors.affiliation: Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
authors.forename: Michael
authors.identifier: 0000-0001-9186-5242
authors.initials: M
authors.lastname: Carey
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D056850:Cyclin-Dependent Kinase 8 / Q000235:genetics / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism*; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C453799:Cbx3 protein, mouse; D002868:Chromosomal Proteins, Non-Histone; D056892:Mediator Complex; D034741:RNA, Small Interfering; D056850:Cyclin-Dependent Kinase 8
keywords: Cbx3; Med26; embryonic stem cell; mesoderm; neural precursor; preinitiation complex