title: Dual-Functional Polyethylene Glycol-b-polyhexanide Surface Coating with in Vitro and in Vivo Antimicrobial and Antifouling Activities.
abstract: In recent years, microbial colonization on the surface of biomedical implants/devices has become a severe threat to human health. Herein, surface-immobilized guanidine derivative block copolymers create an antimicrobial and antifouling dual-functional coating. We report the preparation of an antimicrobial and antifouling block copolymer by the conjugation of polyhexanide (PHMB) with either allyl glycidyl ether or allyloxy polyethylene glycol (APEG; MW 1200 and 2400). The allyl glycidyl ether modified PHMB (A-PHMB) and allyloxy polyethylene glycol1200/2400modified PHMB (APEG1200/2400-PHMB) copolymers were grafted onto a silicone rubber surface as a bottlebrush-like coating, respectively, using a plasma-UV-assisted surface-initiated polymerization. Both A-PHMB and APEG1200/2400-PHMB coatings exhibited excellent broad-spectrum antimicrobial properties against Gram-negative/positive bacteria and fungi. The APEG2400-PHMB coating displayed an improved antibiofilm as well as antifouling properties and a long reusable cycle, compared with two other coatings, due to its abundant PEG blocks among those copolymers. Also, the APEG2400-PHMB-coated silicone coupons were biocompatible toward mammalian cells, as revealed by in vitro hemocompatibile and cytotoxic assays. An in vivo study showed a significant decline of Escherichia coli colonies with a 5-log reduction, indicating the APEG2400-PHMB coating surface worked effectively in the rodent subcutaneous infection model. This PHMB-based block copolymer coating is believed to be an effective strategy to prevent biomaterial-associated infections.
authors.affiliation: Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech) , Nanjing 211816, China.|Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University , Melbourne, Victoria 3086, Australia.
authors.forename: Zelun
authors.initials: Z
authors.lastname: Zhi
authors.affiliation: Center for Biomedical Engineering and Regenerative Medicine, Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an 710049, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Su
authors.affiliation: Center for Biomedical Engineering and Regenerative Medicine, Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an 710049, China.
authors.forename: Yuewei
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech) , Nanjing 211816, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tian
authors.affiliation: Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech) , Nanjing 211816, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Xu
authors.affiliation: Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech) , Nanjing 211816, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.forename: Sara
authors.lastname: Pandidan
authors.affiliation: Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University , Melbourne, Victoria 3086, Australia.
authors.forename: Sara
authors.initials: S
authors.lastname: Padidan
authors.affiliation: Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech) , Nanjing 211816, China.
authors.forename: Peng
authors.identifier: 0000-0002-5876-2177
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech) , Nanjing 211816, China.
authors.forename: Wei
authors.identifier: 0000-0001-7004-6408
authors.initials: W
authors.lastname: Huang
keywords: antibiofilm; antifouling; antimicrobial; polyhexamethylene biguanide (PHMB); surface coating