title: The Mast Cell Antibody-Dependent Degranulatory Synapse.
abstract: Mast cells are key effector cells in inflammation that can be activated by specific antigens via IgE or IgG binding on their FcR. Aggregation of mast cell Fc receptors by cell-bound antigens induces mast cell polarized degranulation toward the stimulatory cell, a process named antibody-dependent degranulatory synapse (ADDS). This polarized degranulation allows mast cells to expose bioactive material embedded in the granule matrix toward the antibody-targeted cell and is accompanied by the formation of a signaling area at the cell-cell contact site. In this chapter, we describe (1) how to stimulate mast cells with cell-bound antigens and (2) how to monitor ADDS formation and to investigate ADDS characteristics by confocal microscopy.
authors.affiliation: INSERM U1043, Centre de physiopathologie de Toulouse Purpan (CPTP), Toulouse, F-31300, France.|Université de Toulouse, Université Paul Sabatier (UPS), Toulouse, F-31062, France.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Valitutti
authors.affiliation: INSERM U1043, Centre de physiopathologie de Toulouse Purpan (CPTP), Toulouse, F-31300, France.|Université de Toulouse, Université Paul Sabatier (UPS), Toulouse, F-31062, France.
authors.forename: Régis
authors.initials: R
authors.lastname: Joulia
authors.affiliation: INSERM U1043, Centre de physiopathologie de Toulouse Purpan (CPTP), Toulouse, F-31300, France. eric.espinosa@inserm.fr.|Université de Toulouse, Université Paul Sabatier (UPS), Toulouse, F-31062, France. eric.espinosa@inserm.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Espinosa
mesh_terms: D000941:Antigens / Q000276:immunology; D015550:Cell Degranulation / Q000276:immunology*; D021961:Cell Membrane Structures / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D018613:Microscopy, Confocal / Q000379:methods; D011961:Receptors, Fc / Q000276:immunology
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D011961:Receptors, Fc; D007073:Immunoglobulin E
keywords: Avidin; Confocal microscopy; Degranulatory synapse; Mast cell; Polarized degranulation