title: Resonant Waveguide Grating Imager for Single Cell Monitoring of the Invasion of 3D Speheroid Cancer Cells Through Matrigel.
abstract: The invasion of cancer cells through their surrounding extracellular matrices is the first critical step to metastasis, a devastating event to cancer patients. However, in vitro cancer cell invasion is mostly studied using two-dimensional (2D) models. Three-dimensional (3D) multicellular spheroids may offer an advantageous cell model for cancer research and oncology drug discovery. This chapter describes a label-free, real-time, and single-cell approach to quantify the invasion of 3D spheroid colon cancer cells through Matrigel using a spatially resolved resonant waveguide grating imager.
authors.affiliation: Biochemical Technologies, Corning Research and Development Corporation, Corning Incorporated, Sullivan Park, Corning, NY, 14831, USA.|NanoScience Technology Center, Department of Mechanical, Materials and Aerospace Engineering, University of Central Florida, Orlando, FL, USA.
authors.forename: Nicole K
authors.initials: NK
authors.lastname: Febles
authors.affiliation: Biochemical Technologies, Corning Research and Development Corporation, Corning Incorporated, Sullivan Park, Corning, NY, 14831, USA.|Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
authors.forename: Siddarth
authors.initials: S
authors.lastname: Chandrasekaran
authors.affiliation: Biochemical Technologies, Corning Research and Development Corporation, Corning Incorporated, Sullivan Park, Corning, NY, 14831, USA. fangy2@corning.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Fang
mesh_terms: D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement; D003094:Collagen; D004338:Drug Combinations; D006801:Humans; D007797:Laminin; D057054:Molecular Imaging / Q000295:instrumentation* / Q000379:methods*; D011509:Proteoglycans; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods*; D018874:Spheroids, Cellular; D014407:Tumor Cells, Cultured
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen
keywords: Adhesion; Colorectal cancer cell; Dynamic mass redistribution; Extracellular matrix; Invasion; Migration; Multicellular spheroid; PTEN; Resonant waveguide grating