title: A network-based method for mechanistic investigation of Shexiang Baoxin Pill's treatment of cardiovascular diseases.
abstract: Shexiang Baoxin Pill (SBP), a traditional Chinese medicine formula, is commonly used to treat cardiovascular disease (CVD) in China. However, the complexity of composition and targets has deterred our understanding of its mechanism of action. Using network pharmacology-based approaches, we established the mechanism of action for SBP to treat CVD by analyzing protein-protein interactions and pathways. The computational results were confirmed at the gene expression level in microarray-based studies. Two of the SBP's targets were further confirmed at the protein level by Western blot. In addition, we validated the theory that SBP's plasma absorbed compounds play major therapeutic role in treating CVD.
authors.affiliation: Department of Mathematics, Logistical Engineering University, Chongqing, China.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Fang
authors.affiliation: Department of Natural Medicinal Chemistry, Second Military Medical University, Shanghai, China.
authors.forename: Hua-Wu
authors.initials: HW
authors.lastname: Zeng
authors.affiliation: Department of Mathematics, Logistical Engineering University, Chongqing, China.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Lin
authors.affiliation: Department of Mathematics, Logistical Engineering University, Chongqing, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Mathematics, Logistical Engineering University, Chongqing, China.
authors.forename: Xiao-Na
authors.initials: XN
authors.lastname: Shen
authors.affiliation: Department of Natural Medicinal Chemistry, Second Military Medical University, Shanghai, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Fu
authors.affiliation: Department of Natural Medicinal Chemistry, Second Military Medical University, Shanghai, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lv
authors.affiliation: Department of Natural Medicinal Chemistry, Second Military Medical University, Shanghai, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Natural Medicinal Chemistry, Second Military Medical University, Shanghai, China.
authors.forename: Run-Hui
authors.initials: RH
authors.lastname: Liu
authors.affiliation: Department of Natural Medicinal Chemistry, Second Military Medical University, Shanghai, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
authors.affiliation: Department of Mathematics, Logistical Engineering University, Chongqing, China.|Department of Natural Medicinal Chemistry, Second Military Medical University, Shanghai, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
mesh_terms: D002318:Cardiovascular Diseases / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks; D006801:Humans; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D015203:Reproducibility of Results; D015398:Signal Transduction
chemical_list: D004365:Drugs, Chinese Herbal; C526712:shexiang baoxin