title: Proteomic Profiling of the Microsomal Root Fraction: Discrimination of Pisum sativum L. Cultivars and Identification of Putative Root Growth Markers.
abstract: Legumes are a large and economically important family, containing a variety of crop plants. Alongside different cereals, some fruits, and tropical roots, a number of leguminosae evolved for millennia as crops with human society. One of these legumes isPisum sativumL., the common garden pea. In the past, breeding has been largely selective on improved above-ground organs. However, parameters, such as root-growth, which determines acquisition of nutrients and water, have largely been underestimated. Although the genome ofP. sativumis still not fully sequenced, multiple proteomic studies have been published on a variety of physiological aspects in the last years. The presented work focused on the connection between root length and the influence of the microsomal root proteome of four different pea cultivars after five days of germination (cultivar Vroege, Girl from the Rhineland, Kelvedon Wonder, and Blauwschokker). In total, 60 proteins were identified to have significantly differential abundances in the four cultivars. Root growth of five-days old seedlings and their microsomal proteome revealed a similar separation pattern, suggesting that cultivar-specific root growth performance is explained by differential membrane and ribosomal protein levels. Hence, we reveal and discuss several putative root growth protein markers possibly playing a key role for improved primary root growth breeding strategies.
authors.affiliation: Oxidative Stress and Plant Proteomics Group, Biocenter Klein Flottbek and Botanical Garden, University of Hamburg, Ohnhorststraße 18, D-22609 Hamburg, Germany. c.meisrimler@uu.nl.|Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. c.meisrimler@uu.nl.
authors.forename: Claudia-Nicole
authors.initials: CN
authors.lastname: Meisrimler
authors.affiliation: Deptartment of Ecogenomics and Systems Biology, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. stefanie.wienkoop@univie.ac.at.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wienkoop
authors.affiliation: Oxidative Stress and Plant Proteomics Group, Biocenter Klein Flottbek and Botanical Garden, University of Hamburg, Ohnhorststraße 18, D-22609 Hamburg, Germany. s.luthje@botanik.uni-hamburg.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Lüthje
keywords: Pisum sativum; cultivar comparison; microsomes; root morphology