title: Sample Clean-up Strategies for ESI Mass Spectrometry Applications in Bottom-up Proteomics: Trends from 2012 to 2016.
abstract: Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favored over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sample or produced during tryptic digestion are detrimental in MS analysis and affect the proteome data, thus sample preparation for removal of these unwanted components has become essential. Here, we review 48 research papers on strategies for removal of salts and surfactants (in particular, SDS) prior to ESI-MS analysis in bottom-up proteomics from 2012 to 2016. The strategies were mostly based on SPE and membrane-based filter-aided sample preparation for salt and SDS removal, respectively. Some known limitations of SPE and filter-aided sample preparation procedures are that they can be time consuming, laborious, and require the use of organic solvents before a concentrated extract suitable for analysis is obtained. The development of faster analytical methods by reducing the sample preparation time and thereby, increasing sample throughput, and in a solvent-less and membrane-less operation, is a significant contribution to proteome research.
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania, Australia.
authors.forename: Ria Marni
authors.initials: RM
authors.lastname: Tubaon
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania, Australia.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Haddad
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania, Australia.
authors.forename: Joselito P
authors.initials: JP
authors.lastname: Quirino
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D011232:Chemical Precipitation; D010455:Peptides / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012492:Salts / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D010455:Peptides; D020543:Proteome; D012492:Salts; D012997:Solvents; D013501:Surface-Active Agents
keywords: ESI-MS; SDS removal; bottom-up proteomics; desalting; sample preparation