title: Association of MAP4K4 gene single nucleotide polymorphism with mastitis and milk traits in Chinese Holstein cattle.
abstract: The objective of the studies presented in this Research Communication was to investigate the association of single nucleotide polymorphisms present in the MAP4K4 gene with different milk traits in dairy cows. Based on previous QTL fine mapping results on bovine chromosome 11, the MAP4K4 gene was selected as a candidate gene to evaluate its effect on somatic cell count and milk traits in ChineseHolstein cows. Milk production traits including milk yield, fat percentage, and protein percentage of each cow were collected using 305 d lactation records. Association between MAP4K4 genotype and different traits and Somatic Cell Score (SCS) was performed using General Linear Regression Model of R. Two SNPs at exon 18 (c.2061T > G and c.2196T > C) with genotype TT in both SNPs were found significantly higher for somatic SCS. We found the significant effect of exon 18 (c.2061T > G) on protein percentage, milk yield and SCS. We identified SNPs at different location of MAP4K4 gene of the cattle and several of them were significantly associated with the somatic cell score and other different milk traits. Thus, MAP4K4 gene could be a useful candidate gene for selection of dairy cattle against mastitis and the identified polymorphisms might potentially be strong genetic markers.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Bhattarai
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Zia
authors.initials: Z
authors.lastname: Ur Rehman
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Hao
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Farman
authors.initials: F
authors.lastname: Ullah
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Rahim
authors.initials: R
authors.lastname: Dad
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Hira Sajjad
authors.initials: HS
authors.lastname: Talpur
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Ishwari
authors.initials: I
authors.lastname: Kadariya
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cui
authors.affiliation: Renmin Hospital of Wuhan University,Wuhan 430060,People's Republic of China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Fan
authors.affiliation: Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Education Ministry of China,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002452:Cell Count; D002681:China; D002874:Chromosome Mapping; D005091:Exons / Q000235:genetics; D005223:Fats / Q000032:analysis; D005260:Female; D005819:Genetic Markers; D007774:Lactation / Q000235:genetics*; D016014:Linear Models; D008414:Mastitis, Bovine / Q000201:enzymology / Q000235:genetics*; D008892:Milk / Q000737:chemistry* / Q000166:cytology; D008894:Milk Proteins / Q000032:analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D040641:Quantitative Trait Loci
chemical_list: D005223:Fats; D005819:Genetic Markers; D008894:Milk Proteins; D017346:Protein Serine-Threonine Kinases
keywords: MAP4K4; mastitis; polymorphism; somatic cell scores