title: [Correlation between the mRNA expression of tissue inhibitor of metalloproteinase-1 and apparent diffusion coefficient on diffusion-weighted imaging in rats' liver fibrosis].
abstract: To explore the correlation between the apparent diffusion coefficient (ADC) and mRNA expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) in different stages of liver fibrosis in rats.  Methods: A model of liver fibrosis in rats was established by intraperitoneal injection of high-fat diet combined with porcine serum. After drug administration for 4 weeks, 48 rats served as a model group and 12 rats served as a control group, then they underwent diffusion weighted imaging (DWI) scanning. The value of ADC was calculated at b value=800 s/mm2. The rats were sacrificed and carried out pathologic examination after DWI scanning immediately. The mRNA expression of TIMP-1 was detected by real time-polymerase chain reaction (RT-PCR). The rats of hepatic fibrosis were also divided into a S0 group (n=4), a S1 group (n=11), a S2 group (n=12), a S3 group (n=10), and a S4 group (n=9) according to their pathological stage. The value of ADC and the expression of TIMP-1 mRNA among the different stage groups of liver fibrosis were compared, and the correlation between ADC and the TIMP-1 mRNA were analyzed.  Results: The ADC value and the TIMP-1 mRNA expression were significantly different between the control group and the liver fibrosis group (F=46.54 and 53.87, P<0.05). There were significant differences in the value of ADC between every two groups (all P<0.05), except the control group vs the S1 group, the S1 group vs the S2 group, and the S2 group vs the S3 group (all P>0.05). For the comparison of TIMP-1 mRNA, there was no significant difference between the S1 group and the S2 group, the S3 group and the S4 group (both P>0.05). There were significant differences among the rest of the groups (all P<0.05). Rank correlation analysis showed that there was a negative correlation between the ADC value and the TIMP-1 mRNA expression (r=-0.76, P<0.01).  Conclusion: When the value of ADC decreases in the progress of rats' liver fibrosis, the mRNA expression of TIMP-1 increases gradually, and there is a negative correlation between them.
authors.affiliation: Department of Radiology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Yuefu
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Department of General Surgery, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Radiology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Radiology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Central Laboratory, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Tan
authors.affiliation: Department of Pathology, Affiliated Hospital of Hainan Medical Collage, Haikou 570102, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Radiology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D038524:Diffusion Magnetic Resonance Imaging; D008099:Liver / Q000737:chemistry*; D008103:Liver Cirrhosis / Q000000981:diagnostic imaging*; D051381:Rats; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000737:chemistry*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1