title: Study of γ-H2AX as DNA double strand break biomarker in resident living in high natural radiation area of Mamuju, West Sulawesi.
abstract: High expression of phospho histone γ-H2AX, a sensitive marker of double stranded DNA damage, is believed to be an indication of defective DNA repair pathway or genomic instability that may cause mutations and ultimately cancer. DNA damage can be caused by ionizing radiation exposure. Beside in medical treatment/diagnosis or industry, ionizing radiation exposure can also be found in naturally in regions of high natural back ground radiation. In this study we collect the blood from 45 volunteers living in Mamuju, a region with highest natural radiation in Indonesia (dose of ∼7 mSv/year). Subjects were grouped as high natural background area (HNBA) (n = 37) and control area (n = 8). The expression γ-H2AX foci were evaluated by one of researcher fluorescence microscope examination. Our results show that the average foci numbers per cell were in the normal range. While not statistical different, the average of γ-H2AX foci in exposed area higher in the exposed compared to the control area, 0.31 versus 0.13 (p > 0.05), respectively. Moreover, there was also no statistical difference of average γ-H2AX foci between man and woman, old and young people in exposed and control area (p > 0.05). In this preliminary study we find that γ-H2AX foci (and thus DNA double strand break) frequency in residents living in the HNBA of Mamuju, West Sulawesi, show a trend towards higher (albeit not significant) average values relative to the control area. More research is needed to further scrutinize these observations.
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia. Electronic address: kurnia@batan.go.id.
authors.forename: Iin Kurnia
authors.initials: IK
authors.lastname: Hasan Basri
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Darlina
authors.initials: D
authors.lastname: Yusuf
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Tur
authors.initials: T
authors.lastname: Rahardjo
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Siti
authors.initials: S
authors.lastname: Nurhayati
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Devita
authors.initials: D
authors.lastname: Tetriana
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Dwi
authors.initials: D
authors.lastname: Ramadhani
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Zubaidah
authors.initials: Z
authors.lastname: Alatas
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Sofiati
authors.initials: S
authors.lastname: Purnami
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Teja
authors.initials: T
authors.lastname: Kisnanto
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Yanti
authors.initials: Y
authors.lastname: Lusiyanti
authors.affiliation: Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency, Jl. Lebakbulus Raya No. 49, Jakarta Indonesia.
authors.forename: Mukh
authors.initials: M
authors.lastname: Syaifudin
mesh_terms: D000328:Adult; D001417:Background Radiation; D015415:Biomarkers / Q000378:metabolism*; D004247:DNA; D053903:DNA Breaks, Double-Stranded; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007214:Indonesia; D008297:Male; D011829:Radiation Dosage; D000069079:Radiation Exposure / Q000706:statistics & numerical data*
chemical_list: D015415:Biomarkers; C468783:H2AX protein, human; D006657:Histones; D004247:DNA
keywords: DNA damage; High background radiation; γ-H2AX