title: Neutralising ability of Terminalia fagifolia extract (Combretaceae) against the in vitro neuromuscular effects of Bothrops jararacussu venom.
abstract: The ability of Terminalia fagifolia hydroalcoholic extract (Tf-HE) to neutralise the paralysis and myotoxicity induced by Bothrops jararacussu venom was assayed using mouse phrenic nerve-diaphragm (PND) preparation and two varieties of chick biventer cervicis (BC) preparations. Tf-HE 100 μg/mL and 500 μg/mL were tested against 40 and 200 μg of venom/mL in PND and BC preparations, respectively, using pre- and post-venom incubation treatments. The effects of Tf-HE against the myotoxicity caused by venom were evaluated via histological analysis (PND) and creatine kinase (CK) release (BC). Tf-HE was able to reverse the venom paralysis in both preparation types. The contractures to exogenous ACh in BC preparations showed that Tf-HE may act on extrinsic, preserving those intrinsic postsynaptic receptors. There was a positive correlation between CK and morphological changes. The high non-hemolytic saponin content can explain the Tf-HE efficacy against the toxic effects of B. jararacussu venom in vertebrate neuromuscular preparations.
authors.affiliation: a Post-Graduate Program in Pharmaceutical Sciences and Pharmacy Graduate Course , University of Sorocaba, (UNISO) , Sorocaba , Brazil.
authors.forename: Natália
authors.initials: N
authors.lastname: Tribuiani
authors.affiliation: a Post-Graduate Program in Pharmaceutical Sciences and Pharmacy Graduate Course , University of Sorocaba, (UNISO) , Sorocaba , Brazil.
authors.forename: Marylu Oliveira
authors.initials: MO
authors.lastname: Tavares
authors.affiliation: a Post-Graduate Program in Pharmaceutical Sciences and Pharmacy Graduate Course , University of Sorocaba, (UNISO) , Sorocaba , Brazil.
authors.forename: Monique Neves
authors.initials: MN
authors.lastname: Santana
authors.affiliation: a Post-Graduate Program in Pharmaceutical Sciences and Pharmacy Graduate Course , University of Sorocaba, (UNISO) , Sorocaba , Brazil.
authors.forename: Isadora Caruso
authors.initials: IC
authors.lastname: Fontana Oliveira
authors.affiliation: a Post-Graduate Program in Pharmaceutical Sciences and Pharmacy Graduate Course , University of Sorocaba, (UNISO) , Sorocaba , Brazil.
authors.forename: Jorge do
authors.initials: JD
authors.lastname: Amaral Filho
authors.affiliation: a Post-Graduate Program in Pharmaceutical Sciences and Pharmacy Graduate Course , University of Sorocaba, (UNISO) , Sorocaba , Brazil.
authors.forename: Magali Glauzer
authors.initials: MG
authors.lastname: Silva
authors.affiliation: b Environmental Sciences Post-Graduate Program, (PGCiamb) , Tocantins Federal University (UFT) , Palmas , Brazil.
authors.forename: Marcio Galdino
authors.initials: MG
authors.lastname: Dos Santos
authors.affiliation: c Post-Graduate Program in Biomedical Engineering and Bioengeneering , University Camilo Castelo Branco (Unicastelo) , Descalvado , Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Cogo
authors.affiliation: d Faculty of Medical Sciences, Department of Pharmacology , State University of Campinas (UNICAMP) , Campinas , Brazil.
authors.forename: Rafael Stuani
authors.initials: RS
authors.lastname: Floriano
authors.affiliation: e Faculty of Pharmaceutical Sciences , State University of Campinas (UNICAMP) , Campinas , Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Cogo-Müller
authors.affiliation: a Post-Graduate Program in Pharmaceutical Sciences and Pharmacy Graduate Course , University of Sorocaba, (UNISO) , Sorocaba , Brazil.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Oshima-Franco
mesh_terms: D000818:Animals; D017837:Bothrops; D002645:Chickens; D003402:Creatine Kinase / Q000378:metabolism; D003435:Crotalid Venoms / Q000633:toxicity*; D003964:Diaphragm / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008297:Male; D051379:Mice; D009465:Neuromuscular Agents / Q000633:toxicity; D010791:Phrenic Nerve / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D029766:Terminalia / Q000737:chemistry*
chemical_list: D003435:Crotalid Venoms; D009465:Neuromuscular Agents; D010936:Plant Extracts; D003402:Creatine Kinase
keywords: Medicinal plant; Myotoxicity; Neurotoxicity; Snake venom