title: A putative Toll/interleukin-1 receptor domain protein from Helicobacter pylori is dimeric in solution and interacts with human Toll-like receptor adaptor myeloid differentiation primary response 88.
abstract: Helicobacter pylori, an important human pathogen, is capable of causing persistent infection with minimal immune response. The first line of defense during H. pylori infection is through gastric epithelial cells that present TLR, A family of bacterial proteins that share homology with the Toll/IL-1 receptor (TIR) domain were identified. Bacterial TIR proteins (BTP) mimic human TIR domain proteins and act on myeloid differentiation primary response gene 88 (MyD88) signaling pathways to suppress TLR signaling. H. pylori may also produce a similar protein. A putative H. pylori BTP was found based on sequence homology. The corresponding gene hp1437 was inserted into an expression vector in fusion with an N-terminal cleavable 6his-tag. The recombinant protein, 6his-HP1437, was purified using nickel affinity chromatography with a yield of 8 mg/L culture. Oligomerization of HP1437 was investigated by size-exclusion chromatography. It was found that HP1437 forms dimers in solution similar to other BTPs. Furthermore, glutathione S-transferase pull down assays identified an interaction between HP1437 and human TIR domain adaptor MyD88. These findings suggest that HP1437 has the characteristic features of BTPs and may play a direct role in reducing immune response against H. pylori by binding to MyD88 and pave the way for an in-depth characterization of this putative novel H. pylori virulence factor.
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Ege University, 35100, Bornova, İzmir, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Kaplan-Türköz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism*; D011485:Protein Binding; D055503:Protein Multimerization; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D051193:Toll-Like Receptors
keywords: Helicobacter pylori; MyD88; TIR domain protein; TLR signaling