title: Depolymerization of Pseudomonas stutzeri exopolysaccharide upon fermentation as a promising production process of antibacterial compounds.
abstract: Many researchers have focused on high molecular weight (Mw) exopolysaccharides (EPS) as a source of potentially bioactive lower Mwderivatives. Therefore, it is of interest to find means for efficient and safe production of depolymerized-polymer derivatives. Exopolysaccharide-depolymerization products (EDP) varying in molecular weight were recovered from fermentative depolymerization of a native EPS produced by Pseudomonas stutzeri AS22. Following their purification and physicochemical characterization, the antibacterial activity of EDP on food spoilage and food poisoning microorganisms was evaluated through the measurement of the inhibition zone diameter, the half maximal (IC50) and the minimal (MIC) inhibitory concentrations. Our results indicate that the lower the Mw, the higher will be the effectiveness of EDP on reducing Gram-negative bacteria growth and the opposite trend was observed in the case of Gram-positive bacteria. EDP bioactivities may provide novel insights into the potentiality of P. stutzeri EPS and its derivatives to be used as functional-food components.
authors.affiliation: Enzyme Engineering and Microbiology Laboratory, Sfax-University, National School of Engineering of Sfax (ENIS), BP 1173-3038 Sfax, Tunisia. Electronic address: hannou25@yahoo.fr.
authors.forename: Hana
authors.initials: H
authors.lastname: Maalej
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., and Université Joseph Fourier, BP 53 Grenoble cedex 9 38041, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Boisset
authors.affiliation: Enzyme Engineering and Microbiology Laboratory, Sfax-University, National School of Engineering of Sfax (ENIS), BP 1173-3038 Sfax, Tunisia.
authors.forename: Noomen
authors.initials: N
authors.lastname: Hmidet
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., and Université Joseph Fourier, BP 53 Grenoble cedex 9 38041, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Colin-Morel
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., and Université Joseph Fourier, BP 53 Grenoble cedex 9 38041, France.
authors.forename: Laurine
authors.initials: L
authors.lastname: Buon
authors.affiliation: Enzyme Engineering and Microbiology Laboratory, Sfax-University, National School of Engineering of Sfax (ENIS), BP 1173-3038 Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D005285:Fermentation; D008970:Molecular Weight; D058105:Polymerization; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D011135:Polysaccharides, Bacterial; C064841:exopolysaccharide, Pseudomonas
keywords: Antibacterial activity; EPS-derivatives; Exopolysaccharide; Fermentative depolymerization; Molecular weight; P. stutzeri AS22