title: Hydrophobically modified polyethylenimine-based ternary complexes for targeting brain tumor: stability, in vitro and in vivo studies.
abstract: Hydrophobic modification of low molecular weight polyethylenimine (PEI 2 kDa) by cholic acid (ChA) was done to obtain PEI2-ChA. The nanoplexes of PEI2-ChA with gWIZ-GFP demonstrated increase transfection efficiency (∼27%) in NT8e cell lines. The cell-cycle analysis of NT8e cells (p53 mutant) treated with transferrin containing nanoplexes showed increased apoptosis of cells. In vitro protein expression revealed expression of exogenous p53 protein. In vivo imaging of mice showed localized signal for GFP protein in brain region. The tumors of mice treated with transferrin containing nanoplexes of PEI2-ChA were ∼5 times smaller in size than the tumor of untreated animals.
authors.affiliation: a Pharmacy Department, Faculty of Pharmacy , The M.S. University of Baroda , Kalabhavan, Vadodara , India.
authors.forename: Brahmanand
authors.initials: B
authors.lastname: Dube
authors.affiliation: a Pharmacy Department, Faculty of Pharmacy , The M.S. University of Baroda , Kalabhavan, Vadodara , India.
authors.forename: Abhijeet
authors.initials: A
authors.lastname: Pandey
authors.affiliation: b Genetic Engineering Lab , ACTREC Tata Memorial Centre , Kharghar, Navi Mumbai , India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Joshi
authors.affiliation: a Pharmacy Department, Faculty of Pharmacy , The M.S. University of Baroda , Kalabhavan, Vadodara , India.
authors.forename: Krutika
authors.initials: K
authors.lastname: Sawant
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D019826:Cholic Acid / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D058990:Molecular Targeted Therapy; D008970:Molecular Weight; D011094:Polyethyleneimine / Q000737:chemistry*; D014018:Tissue Distribution; D014168:Transferrin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D023041:Xenograft Model Antitumor Assays
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004337:Drug Carriers; D014168:Transferrin; D011094:Polyethyleneimine; D019826:Cholic Acid
keywords: Polyethylenimine; biodistribution; cholic acid; ternary complex; transferrin; tumor regression