title: The role of MALAT-1 in the invasion and metastasis of gastric cancer.
abstract: OBJECTIVE
  The long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) has been reported to be over-expressed in several cancer types. However, its role in gastric cancer (GC) remains unclear. In the present study, we examined the expression of MALAT-1 in GC cells and tissues and explored its role in GC cell migration and invasion.
  
  
  METHODS
  Real-time quantitative polymerase chain reaction (qRT-PCR) was used to analyze the expression level of MALAT-1 in six GC cell lines and 20 gastric tissues (20 GC and 20 adjacent normal mucosa). Functional characterization for the role of MALAT-1 in GC was performed by small interfering RNA (siRNA) knockdown, followed by series of in vitro and in vivo experiments.
  
  
  RESULTS
  MALAT-1 was upregulated in GC cell lines and tissues compared with the immortalized gastric epithelial cell line GES and adjacent normal tissues, respectively. Moreover, MALAT-1 expression was higher in the high-metastatic-potential GC cell line SGC7901M than in the low-metastatic-potential GC cell line SGC7901NM. In vitro and in vivo assays showed that siRNA-mediated silencing of MALAT-1 inhibited GC cell migration and invasion. In addition, suppressing MALAT-1 expression resulted in a decrease in the expression of the Epithelial-mesenchymal transition (EMT)-associated marker vimentin and an increase in the expression of E-cadherin at both the mRNA and protein levels.
  
  
  CONCLUSIONS
  MALAT-1 may promote the migration and invasion of GC cells in part by regulating EMT.
authors.affiliation: a State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases , the Fourth Military Medical University , Xi'an , China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: b Department of Oncology , Tangdu Hospital, the Fourth Military Medical University , Xi'an , China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.affiliation: c Department of Gastroenterology , the 16th Hospital of the People's Liberation Army of China , A Letai , China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: d Air Force General Hospital of the People's Liberation Army of China , Beijing , China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yu
authors.affiliation: b Department of Oncology , Tangdu Hospital, the Fourth Military Medical University , Xi'an , China.
authors.forename: Yafang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: e Department of Gastroenterology , Xi'an Central Hospital , Xi'an , China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Liu
authors.affiliation: a State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases , the Fourth Military Medical University , Xi'an , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
authors.affiliation: a State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases , the Fourth Military Medical University , Xi'an , China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; C557041:MALAT1 long non-coding RNA, human; C557040:Malat1 long non-coding RNA, mouse; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D014746:Vimentin
keywords: EMT; LncRNA; MALAT-1; gastric cancer; metastasis