title: [Expression of Septin2 in Hodgkin lymphoma cell line L428 and its role in promoting H/RS cells' redifferentiation to B lymphocytes].
abstract: Objective:To explore the role of GTP binding protein 2 (Septin2) in the differentiation of Hodgkin's Lymphoma H/RS cells (Hodgkin/Reed-Sternberg) to B lymphocytes.Methods:The expressions of Septin2 mRNA and protein in Hodgkin's Lymphoma cell line L428 which CD99 was overexpressed were detected by RT-qPCR and Western blot,confocal laser microscopy and immunocytochemistry (ICC) . RT-qPCR and Western blot were used to assay the expression of Septin2 after Septin2-siRNA transfected into L428 cells, and confocal laser microscopy, CCK8 assay and flow cytometry were used to analyze the changes of F-actin cytoskeleton,cell proliferation ability and immunophenotype.Results:The low expressions of Septin2 mRNA and protein were detected in L428 cell line after overexpresion of CD99 (0.329±0.019vs1.000,P=0.001) and Septin2 siRNA transfection (0.276±0.025vs1.000,P=0.000) compared to controls. Compared to vector group, Septin2 siRNA transfection could lead to decrease of cell size, decline of proliferation activity (F=204.927,P<0.001) and reconstruction of F-actin cytoskeleton, and the expression of specific antigen markers CD30 and CD15 of H/RS cell decreased, B cell antigen marker CD19 as well as germinal center marker CD10 and early plasma cell marker CD38 were up-regulated.Conclusion:Septin2 interference promotes H/RS cells' redifferentiation to B lymphocytes.
authors.affiliation: Department of Pathology, Southern Medical University, Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, China.
authors.forename: Q C
authors.initials: QC
authors.lastname: Sun
authors.forename: L
authors.initials: L
authors.lastname: Zhong
authors.forename: B
authors.initials: B
authors.lastname: Qiu
authors.forename: T
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Southern Medical University, Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Zhou
mesh_terms: D000071443:12E7 Antigen; D000199:Actins; D001402:B-Lymphocytes; D002452:Cell Count; D002460:Cell Line; D005434:Flow Cytometry; D006689:Hodgkin Disease; D006801:Humans; D016130:Immunophenotyping; D034741:RNA, Small Interfering; D016539:Reed-Sternberg Cells; D014162:Transfection; D015854:Up-Regulation
chemical_list: D000071443:12E7 Antigen; D000199:Actins; C061828:CD99 protein, human; D034741:RNA, Small Interfering
keywords: Cell differentiation; Hodgkin disease; L428 cells; Reed-Sternberg cells; Septins