title: Association ofNFKB1gene polymorphism (rs28362491) with levels of inflammatory biomarkers and susceptibility to diabetic nephropathy in Asian Indians.
abstract: OBJECTIVE
  To investigate the association ofNFKB1gene -94 ATTG insertion/deletion (rs28362491) polymorphism with inflammatory markers and risk of diabetic nephropathy in Asian Indians.
  
  
  METHODS
  A total of 300 subjects were recruited (100 each), normoglycemic, (NG); type 2 diabetes mellitus (T2DM) without any complications (DM) and T2DM with diabetic nephropathy [DM-chronic renal disease (CRD)]. Analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism and ELISA. Pearson's correlation, analysis of variance and logistic regression were used for statistical analysis.
  
  
  RESULTS
  The allelic frequencies of -94 ATTG insertion/deletion were 0.655/0.345 (NG), 0.62/0.38 (DM) and 0.775/0.225 (DM-CRD). The -94 ATTG ins allele was associated with significantly increased levels of urinary monocyte chemoattractant protein-1 (uMCP-1); uMCP-1 (P= 0.026) and plasma tumor necrosis factor-alpha (TNF-α); TNF-α (P= 0.030) and almost doubled the risk of diabetic nephropathy (OR = 1.91, 95%CI: 1.080-3.386,P= 0.025).
  
  
  CONCLUSIONS
  -94 ATTG ins/ins polymorphism might be associated with increased risk of developing nephropathy in Asian Indian subjects with diabetes mellitus.
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Amar
authors.initials: A
authors.lastname: Gautam
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Stuti
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Mohit
authors.initials: M
authors.lastname: Mehndiratta
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Mohini
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Kalpana
authors.initials: K
authors.lastname: Singh
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Om P
authors.initials: OP
authors.lastname: Kalra
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Agarwal
authors.affiliation: Amar Gautam, Om P Kalra, Sunil Agarwal, Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Delhi 110095, India.
authors.forename: Jasvinder K
authors.initials: JK
authors.lastname: Gambhir
keywords: Diabetic nephropathy; Inflammation; NFKB1 -94 ATTG ins/del polymorphism; Tumor necrosis factor-alpha; Urinary monocyte chemoattractant protein-1