title: Relocation of NPM Affects the Malignant Phenotypes of Hepatoma SMMC-7721 Cells.
abstract: Nucleophosmin(NPM), heavily implicated in diverse solid tumors, is an important multifunctional protein mainly located in the nucleolus. Our previous study confirmed that NPM can also localize and accumulate in the cytoplasm of liver cancer cells. However, the role of cytoplasmic NPM (NPMc +) is unclear. Here, we showed that both nucleolar NPM and NPMc+ could promote cell proliferation, although the effect of NPMc+ was weaker than that of NPM. Cell adhesion ability of hepatoma cells was significantly reduced to a greater extent by NPMc+ expression. Nucleolar NPM enhanced cell migration and invasion, whereas NPMc+ impeded cell migration and invasion. The investigation of NPM interactional proteins by proteomic method demonstrated that the NPM was involved in multiple biological processes. By contrast, the interactional proteins of NPMc+ were mainly implicated in tRNA amino acylation regulation. The interactional network of NPMc+ was significantly small and simple. These results suggested that relocation of NPM altered its interactional network and consequently disturbed the primary functions, including cell proliferation, adhesion, migration, and invasion. NPM plays a promotional role in cancer and the reducing relocation may be a potential therapeutic target for hepatocellular carcinoma. J. Cell. Biochem. 118: 3225-3236, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Basic Medicine, Medical College of Xiamen University/Cancer Research Center of Xiamen University, Xiamen, 361102, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Hepatic Biliary Pancreatic Vascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, Fujian, P.R. China.
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Xu
authors.affiliation: Department of Basic Medicine, Medical College of Xiamen University/Cancer Research Center of Xiamen University, Xiamen, 361102, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University/Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian, P.R. China.
authors.forename: Guo-Yan
authors.initials: GY
authors.lastname: Liu
authors.affiliation: Department of Basic Medicine, Medical College of Xiamen University/Cancer Research Center of Xiamen University, Xiamen, 361102, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lu
authors.affiliation: Department of Basic Medicine, Medical College of Xiamen University/Cancer Research Center of Xiamen University, Xiamen, 361102, P.R. China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Deng
authors.affiliation: Department of Basic Medicine, Medical College of Xiamen University/Cancer Research Center of Xiamen University, Xiamen, 361102, P.R. China.
authors.forename: Qi-Fu
authors.initials: QF
authors.lastname: Li
authors.affiliation: Department of Basic Medicine, Medical College of Xiamen University/Cancer Research Center of Xiamen University, Xiamen, 361102, P.R. China.
authors.forename: Song-Lin
authors.identifier: 0000-0003-1213-0550
authors.initials: SL
authors.lastname: Shi
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D021381:Protein Transport
chemical_list: C000716967:NPM1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000090243:Nucleophosmin
keywords: CELLULAR LOCALIZATION; INTERACTIONAL PROTEINS; LIVER CANCER; NPM