title: [The composition of the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora].
abstract: Objective:To explore the composition of the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora.Method:Twenty-four specimens were collected from pregnant Kunming mouse including 8 mice of early embryonic (12-13 days) gastrointestinal tissues, 8 cases of late embryonic (19-20 days)gastrointestinal tissues, 8 of late pregnancy placental tissues.The 24 samples were extracted by DNeasy Blood & Tissue kit for high-throughput DNA sequencing.Result:The level of Proteobacteria, Bacteroidetes, Actino-bacteria and Firmicutes were predominantin all specimens.The relative content of predominant bacterial phyla in each group: Proteobacteria (95.00%, 88.14%, 87.26%), Bacteroidetes(1.71%, 2.15%, 2.63%), Actino-Bacteria(1.16%, 4.10%, 3.38%), Firmicutes(0.75%, 2.62%, 2.01%). At the level of family, there were nine predominant bacterial families in whichEnterobacteriaeae,Shewanel laceaeandMoraxellaceaewere dominant.The relative content of dominant bacterial family in eachgroup:Enterobacteriaeae(46.99%, 44.34%, 41.08%),Shewanellaceae(21.99%, 21.10%, 19.05%),Moraxellaceae(9.18%, 7.09%, 5.64%). From the species of flora, the flora from fetal gastrointestinal in early pregnancy and late pregnancy (65.44% and 62.73%) were the same as that from placenta tissue in the late pregnancy.From the abundance of bacteria, at the level of family, the same content of bacteria in three groups accounted for 78.16%, 72.53% and 65.78% respectively.Conclusion:It was proved that the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora were colonized. At the same time the bacteria are classified.
authors.affiliation: Department of Pediatrics, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, China.
authors.forename: D
authors.initials: D
authors.lastname: Lei
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.forename: X
authors.initials: X
authors.lastname: Jiang
authors.forename: L
authors.initials: L
authors.lastname: Lan
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: B X
authors.initials: BX
authors.lastname: Wang
mesh_terms: D000818:Animals; D001419:Bacteria; D041963:Bacteroidetes; D004269:DNA, Bacterial; D004622:Embryo, Mammalian; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D010920:Placenta / Q000382:microbiology*; D011247:Pregnancy; D012336:RNA, Ribosomal, 16S
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Bacteria; Gastrointestinal tract; High-throughput nucleotide sequencing; Mice; Placenta