title: Nanoplasmonic Biosensor Using Localized Surface Plasmon Resonance Spectroscopy for Biochemical Detection.
abstract: Localized surface plasmon resonance (LSPR) associated with metal nanostructures has developed into a highly useful sensor technique. Optical LSPR spectroscopy of nanostructures often shows sharp absorption and scattering peaks, which can be used to probe several bio-molecular interactions. Here, we report nanoplasmonic biosensors using LSPR on nanocup arrays (nanoCA) to recognize bio-molecular binding for biochemical detection. These sensors can be modified to quantify binding of small molecules to proteins for odorant and explosive detections. Electrochemical LSPR biosensors can also be designed by coupling electrochemistry and LSPR spectroscopy measurements. Multiple sensing information can be obtained and electrochemical LSPR property can be investigated for biosensors. In some applications, the electrochemical LSPR biosensor can be used to quantify immunoreactions and enzymatic activity. The biosensors exhibit better performance than those of conventional optical LSPR measurements. With multi-transducers, the nanoplasmonic biosensor can provide a promising approach for bio-detection in environmental monitoring, healthcare diagnostics, and food quality control.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Diming
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China. qjliu@zju.edu.cn.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004798:Enzymes / Q000737:chemistry; D004867:Equipment Design; D019476:Insect Proteins / Q000032:analysis; D008024:Ligands; D008958:Models, Molecular; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis; D011485:Protein Binding; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D015415:Biomarkers; D004798:Enzymes; D019476:Insect Proteins; D008024:Ligands; D010455:Peptides
keywords: BSA (bull serum albumin); Biosensor; Electrochemistry; Localized surface plasmon resonance (LSPR); Nanocup arrays (nanoCA); Thrombin