title: A genetic variant in long non-coding RNA MALAT1 associated with survival outcome among patients with advanced lung adenocarcinoma: a survival cohort analysis.
abstract: Recently studies have demonstrated that the long non-coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) may participate in the development and progression of lung cancer. In this study, we hypothesized that genetic variant of this lncRNA may affect the prognosis of lung cancer patients.
  
  
  
  We conducted a follow-up study for 538 patients with non-small cell lung carcinoma (NSCLC), including 140 early-staged (stage I and II) and 398 advanced staged (stage III and IV) patients. The genetic variant rs3200401 in MALAT1 was then genotyped among this population by using TaqMan assay. The association of this variant with overall survival of these patients was further analyzed.
  
  
  
  It was shown that among the advanced lung adenoma patients, subjects carrying rs3200401 CT and CT + TT genotypes had significantly longer median survival time (MST = 29.9, 28.9 vs. 19.3 month, Long-rank P = 0.019 and 0.024, respectively) and decreased death risks [crude HR (95% CI) = 0.65 (0.43-0.98) and 0.64 (0.44-0.95), P = 0.040 and 0.025, respectively], when compared to subjects wtih the MALAT1 rs3200401 CC genotype. However, the beneficial effect of rs3200401 was not seen among early NSCLC and advanced lung squamous cell carcinoma patients. We further tested the TCGA data, and found that a higher expression of MALAT1 was associated with metastatic of advanced lung adenocarcinoma but not with lung squamous cell carcinoma.
  
  
  
  The rs3200401 T allele located on the lncRNA MALAT1 was associated with a better survival for advanced lung adenocarcinoma patients, which may offer a novel prognostic biomarker for this patient subgroup. However, these results need to be validated in larger populations of lung cancer and the biological function of this variant still warrants further investigation.
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China.
authors.forename: Jing-Jun
authors.initials: JJ
authors.lastname: Xiang
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China.
authors.forename: Li-Ge
authors.initials: LG
authors.lastname: Wu
authors.affiliation: Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd, Wuhan, 430030, China.
authors.forename: Yan-Sen
authors.initials: YS
authors.lastname: Bai
authors.affiliation: Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd, Wuhan, 430030, China.
authors.forename: Zhuo-Wang
authors.initials: ZW
authors.lastname: Chen
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Yin
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China.
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Guo
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd, Wuhan, 430030, China. ghuan5011@hust.edu.cn.
authors.forename: Huan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Oncology, Wuhan Iron and Steel (Group) Corporation Staff-Worker Hospital, 32 Qinghua Road, Wuhan, 430085, China. wgxuping@126.com.|Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd, Wuhan, 430030, China. wgxuping@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: Long non-coding RNA; Lung cancer; Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1); Survival