title: Evaluation of antioxidant activities and chemical analysis of sulfated chitosan from Sepia prashadi.
abstract: The chitin and chitosan of S. prashadi was prepared through demineralization, deproteinzation, deacetylation process and sulfation were carried by chlorosulfonic acid in N,N-dimethylformamide. The sulfate content in chitosan was found to be 18.9%. The carbon, hydrogen and nitrogen composition of the sulfated chitosan were recorded 39.09%, 6.95% and 6.58% respectively. The structural analysis was done by using FT-IR and NMR spectroscopy technique. The DSC curves of sulfated chitosan showed a large endothermic peak resolved with Tovalue of 54.57°C and TPvalue of 97.46°C. The morphology of sulfated chitin and sulfated chitosan were studied by SEM. The Further in vitro antioxidant activity of sulfated chitosan was screened by scavenging activity of superoxide radical assay, hydroxyl radical scavenging assay, metal-ion chelating effect and reducing power. Its anticoagulant activity was tested for human plasma with respect to Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT). Results prove that sulfated chitosan has potent antioxidant and anticoagulant activity.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Electronic address: seedevisalem@gmail.com.
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Seedevi
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Meivelu
authors.initials: M
authors.lastname: Moovendhan
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Vairamani
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Annaian
authors.initials: A
authors.lastname: Shanmugam
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002686:Chitin / Q000032:analysis; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D049833:Sepia / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D013431:Sulfates; D013481:Superoxides; D002686:Chitin; D017665:Hydroxyl Radical; D048271:Chitosan; D007501:Iron
keywords: Antioxidant activity; SEM; Sulfated chitosan