title: In Vitro Selection of DNA Aptamers that Binds Geniposide.
abstract: Geniposide is a key iridoid glycoside from Gardenia jasminoides fructus widely used in traditional Chinese herbal medicine. However, detection of this small molecule represents a significant challenge mostly due to the lack of specific molecular recognition elements. In this study, we have performed in vitro selection experiments to isolate DNA aptamers that can specifically bind geniposide. Using a stringent selection procedure, we have isolated DNA aptamers that can distinguish geniposide from genipin and glucose, two structural analogs of geniposide. Two top aptamers exhibit low micromolar binding affinity towards geniposide, but show significantly reduced affinity to genipin and glucose. These aptamers have the potential to be further developed into analytical tools for the detection of geniposide.
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China. aozhezhang@outlook.com.
authors.forename: Aozhe
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada. changd3@mcmaster.ca.
authors.forename: Dingran
authors.initials: D
authors.lastname: Chang
authors.affiliation: Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China. zhangzijianQQ1012@163.com.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada. lifan@mcmaster.ca.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China. liweihong.403@163.com.
authors.forename: Weihong
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China. wangxubucm@sina.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada. liying@mcmaster.ca.
authors.forename: Yingfu
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China. hqianz@aliyun.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Hua
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D032062:Gardenia / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000737:chemistry; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D039823:Iridoids; D010936:Plant Extracts; C007835:geniposide; C007834:genipin; D005947:Glucose
keywords: Chinese herbal medicine; DNA; aptamer; geniposide; in vitro selection; molecular recognition