title: High-density genetic map and identification of QTLs for responses to temperature and salinity stresses in the model brown alga Ectocarpus.
abstract: Deciphering the genetic architecture of adaptation of brown algae to environmental stresses such as temperature and salinity is of evolutionary as well as of practical interest. The filamentous brown alga Ectocarpus sp. is a model for the brown algae and its genome has been sequenced. As sessile organisms, brown algae need to be capable of resisting the various abiotic stressors that act in the intertidal zone (e.g. osmotic pressure, temperature, salinity, UV radiation) and previous studies have shown that an important proportion of the expressed genes is regulated in response to hyposaline, hypersaline or oxidative stress conditions. Using the double digest RAD sequencing method, we constructed a dense genetic map with 3,588 SNP markers and identified 39 QTLs for growth-related traits and their plasticity under different temperature and salinity conditions (tolerance to high temperature and low salinity). GO enrichment tests within QTL intervals highlighted membrane transport processes such as ion transporters. Our study represents a significant step towards deciphering the genetic basis of adaptation of Ectocarpus sp. to stress conditions and provides a substantial resource to the increasing list of tools generated for the species.
authors.affiliation: Algal Genetics Group, UMR 8227, CNRS, Sorbonne Universités, UPMC, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.|UMI 3614 Evolutionary Biology and Ecology of Algae, CNRS, Sorbonne Universités, UPMC, Pontificia Universidad Católica de Chile, Universidad Austral de Chile, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.
authors.forename: Komlan
authors.initials: K
authors.lastname: Avia
authors.affiliation: Algal Genetics Group, UMR 8227, CNRS, Sorbonne Universités, UPMC, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.
authors.forename: Susana M
authors.initials: SM
authors.lastname: Coelho
authors.affiliation: Algal Genetics Group, UMR 8227, CNRS, Sorbonne Universités, UPMC, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.|UMI 3614 Evolutionary Biology and Ecology of Algae, CNRS, Sorbonne Universités, UPMC, Pontificia Universidad Católica de Chile, Universidad Austral de Chile, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.|Centro de Conservación Marina and CeBiB, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
authors.forename: Gabriel J
authors.initials: GJ
authors.lastname: Montecinos
authors.affiliation: Algal Genetics Group, UMR 8227, CNRS, Sorbonne Universités, UPMC, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Cormier
authors.affiliation: Algal Genetics Group, UMR 8227, CNRS, Sorbonne Universités, UPMC, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.
authors.forename: Fiona
authors.initials: F
authors.lastname: Lerck
authors.affiliation: UMI 3614 Evolutionary Biology and Ecology of Algae, CNRS, Sorbonne Universités, UPMC, Pontificia Universidad Católica de Chile, Universidad Austral de Chile, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Mauger
authors.affiliation: UMI 3614 Evolutionary Biology and Ecology of Algae, CNRS, Sorbonne Universités, UPMC, Pontificia Universidad Católica de Chile, Universidad Austral de Chile, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.|Centro de Conservación Marina and CeBiB, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Faugeron
authors.affiliation: UMI 3614 Evolutionary Biology and Ecology of Algae, CNRS, Sorbonne Universités, UPMC, Pontificia Universidad Católica de Chile, Universidad Austral de Chile, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Valero
authors.affiliation: Algal Genetics Group, UMR 8227, CNRS, Sorbonne Universités, UPMC, Station Biologique Roscoff, CS 90074, 29688 Roscoff, France.
authors.forename: J Mark
authors.initials: JM
authors.lastname: Cock
authors.affiliation: Ifremer, Laboratoire des Sciences de l'Environnement Marin (UMR 6539 LEMAR, UBO, CNRS, IRD, Ifremer), Centre Bretagne - ZI de la Pointe du Diable, CS 10070, 29280 Plouzané, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Boudry
mesh_terms: D000222:Adaptation, Physiological; D004781:Environmental Exposure; D005819:Genetic Markers; D000459:Phaeophyceae / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D054712:Salinity; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D005819:Genetic Markers