title: In-vitro digestion of probiotic bacteria and omega-3 oil co-microencapsulated in whey protein isolate-gum Arabic complex coacervates.
abstract: Solid co-microcapsules of omega-3 rich tuna oil and probiotic bacteria L. casei were produced using whey protein isolate-gum Arabic complex coacervate as wall material. The in-vitro digestibility of the co-microcapsules and microcapsules was studied in terms of survival of L. casei and release of oil in sequential exposure to simulated salivary, gastric and intestinal fluids. Co-microencapsulation significantly increased the survival and surface hydrophobicity and the ability of L. casei to adhere to the intestinal wall. No significant difference in the assimilative reduction of cholesterol was observed between the microencapsulated and co-microencapsulated L. casei. The pattern of release of oil from the microcapsules and co-microcapsules was similar. However, the content of total chemically intact omega-3 fatty acids was higher in the oil released from co-microcapsules than the oil released from microcapsules. The co-microencapsulation can deliver bacterial cells and omega-3 oil to human intestinal system with less impact on functional properties.
authors.affiliation: Faculty of Science and Technology, Federation University Australia, Ballarat, VIC 3353, Australia. Electronic address: d.eratte@federation.edu.au.
authors.forename: Divya
authors.initials: D
authors.lastname: Eratte
authors.affiliation: Faculty of Science and Technology, Federation University Australia, Ballarat, VIC 3353, Australia. Electronic address: k.dowling@federation.edu.au.
authors.forename: Kim
authors.initials: K
authors.lastname: Dowling
authors.affiliation: Centre for Chemistry and Biotechnology, Deakin University, Geelong, VIC 3217, Australia.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
authors.affiliation: School of Science, RMIT, Melbourne, VIC 3083, Australia; CSIRO Manufacturing Flagship, Clayton South, VIC 3169, Australia.
authors.forename: Benu P
authors.initials: BP
authors.lastname: Adhikari
mesh_terms: D001422:Bacterial Adhesion; D004063:Digestion; D004339:Drug Compounding; D016503:Drug Delivery Systems; D015525:Fatty Acids, Omega-3 / Q000737:chemistry* / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D006170:Gum Arabic / Q000737:chemistry*; D006801:Humans; D007780:Lacticaseibacillus casei / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology; D050296:Microbial Viability; D008954:Models, Biological; D019936:Probiotics / Q000032:analysis*; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D015525:Fatty Acids, Omega-3; D000067816:Whey Proteins; D006170:Gum Arabic
keywords: Co-microencapsulation; In-vitro digestion; Omega-3 oil; Probiotic bacteria; Simulated digestive fluids; Survival