title: A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.
abstract: The release of intracellular products, especially polyhydroxyalkanoates, is still a great challenge in industry. To solve this bottleneck, a novel autolysis system strictly controlled with magnesium was constructed and applied to poly(3-hydroxypropionate) production in engineered Escherichia coli. The autolysis system was constructed by inserting the 5'untranslated region (5'UTR) behind promoter PmgtA with lysis genes (S, R, and Rz, from E. coli) overexpressed. The autolysis system functioned well (lysis efficiency of more than 90%) in the P3HP producer with double plasmids containing lysis genes and P3HP biosynthesis genes, whereas the P3HP production was reduced due to plasmid losses. After the autolysis genes and P3HP biosynthesis genes were integrated into one plasmid, the P3HP content of 72.7% (2.4 times of the control) and the plasmid stability of 79.8 ± 3.1% were achieved in strain Q2646 with promoter PmgtA-UTR. However, the strain Q2647 with promoter PmgtA could not accumulate P3HP because of rapid cell lysis. The novel autolysis system activated in Mg2+-depleted conditions proves to be feasible for polyhydroxyalkanoates production, which may have great application potential for other intracellular products.
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.|b Laboratory of Microbiology and Antimicrobials Substances , Department of Biochemistry, Faculty of Sciences, University of Dschang , Dschang , Cameroon.
authors.forename: Stephen
authors.initials: S
authors.lastname: Tamekou Lacmata
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yao
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: b Laboratory of Microbiology and Antimicrobials Substances , Department of Biochemistry, Faculty of Sciences, University of Dschang , Dschang , Cameroon.
authors.forename: Jules-Roger
authors.initials: JR
authors.lastname: Kuiate
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Feng
authors.affiliation: a CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Guang
authors.identifier: http://orcid.org/0000-0003-0002-5972
authors.initials: G
authors.lastname: Zhao
mesh_terms: D001433:Bacteriolysis / Q000187:drug effects / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods; D008274:Magnesium / Q000008:administration & dosage*; D060847:Metabolic Engineering / Q000379:methods*; D011091:Polyesters / Q000302:isolation & purification / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D011091:Polyesters; C000600150:poly(3-hydroxypropionate); D008274:Magnesium
keywords: P3HP; autolysis system; industrial applications; magnesium; promoter; untranslated regions