title: Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d0/d4-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry analysis.
abstract: As the key aroma compounds, varietal thiols are the crucial odorants responsible for the flavor of wines. Quantitative analysis of thiols can provide crucial information for the aroma profiles of different wine styles. In this study, a rapid and sensitive method for the simultaneous determination of six thiols in wine using d0/d4-acridone-10-ethyl-N-maleimide (d0/d4-AENM) as stable isotope-coded derivatization reagent (SICD) by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) has been developed. Quantification of thiols was performed by using d4-AENM labeled thiols as the internal standards (IS), followed by stable isotope dilution HPLC-ESI-MS/MS analysis. The AENM derivatization combined with multiple reactions monitoring (MRM) not only allowed trace analysis of thiols due to the extremely high sensitivity, but also efficiently corrected the matrix effects during HPLC-MS/MS and the fluctuation in MS/MS signal intensity due to instrument. The obtained internal standard calibration curves for six thiols were linear over the range of 25-10,000pmol/L (R2≥0.9961). Detection limits (LODs) for most of analytes were below 6.3pmol/L. The proposed method was successfully applied for the simultaneous determination of six kinds of thiols in wine samples with precisions ≤3.5% and recoveries ≥78.1%. In conclusion, the developed method is expected to be a promising tool for detection of trace thiols in wine and also in other complex matrix.
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China.
authors.forename: Zhengxian
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China; Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China. Electronic address: jmyou6304@163.com.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China.
authors.forename: Shuaimin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China.
authors.forename: Weidi
authors.initials: W
authors.lastname: Sun
authors.affiliation: Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China.
authors.forename: Zhongyin
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China. Electronic address: sunzhiw@126.com.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China.
authors.forename: Cuihua
authors.initials: C
authors.lastname: Song
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, People's Republic of China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.affiliation: Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, People's Republic of China.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Yourui
authors.initials: Y
authors.lastname: Suo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007553:Isotope Labeling; D008301:Maleimides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014920:Wine / Q000032:analysis*
chemical_list: D008301:Maleimides; D013438:Sulfhydryl Compounds
keywords: HPLC-ESI-MS/MS; Stable isotope-coded derivatization (SICD); Thiols; Wine