title: Chemical shift assignments of Ribosomal protein S1 from Mycobacterium tuberculosis.
abstract: RpsA, also known as ribosomal protein S1, is an essential protein required for translation initiation of mRNAs when their Shine-Dalgarno sequence is degenerated (Sorensen et al. 1998). In addition, RpsA of Mycobacterium tuberculosis (M. tb) is involved in trans-translation, which is an effective system mediated by tmRNA-SmpB to release stalled ribosomes from mRNA in the presence of rare codons (Keiler 2008). Shi et al. found that POA binds to RpsA of Mtb and disrupts the formation of RpsA-tmRNA complex (Shi et al. 2011) and mutations at the C-terminus of RpsA confer PZA resistance. The previous work reported the pyrazinoic acid-binding domain of RpsA (Yang et al. Mol Microbiol 95:791-803, 2015). However, the HSQC spectra of the isolated S1 domain does not overlap with that of MtRpsA280-438, suggesting that substantial interactions occur between the flexible C-terminus and the S1 domain in MtRpsA .To further study the PZA resistance and how substantial interactions influence/affect protein structure, using heteronuclear NMR spectroscopy, we have completed backbone and side-chain1H,15N,13C chemical shift assignments of MtRpsA280-438 which contains S1 domain and the flexible C-terminus. These NMR resonance assignments provide the framework for detailed characterization of the solution-state protein structure determination, dynamic studies of this domain, as well as NMR-based drug discovery efforts.
authors.affiliation: High-field NMR Research Center, Key Laboratory of Chemical Biology of Fujian Province, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Fu
authors.affiliation: High-field NMR Research Center, Key Laboratory of Chemical Biology of Fujian Province, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Biling
authors.initials: B
authors.lastname: Huang
authors.affiliation: High-field NMR Research Center, Key Laboratory of Chemical Biology of Fujian Province, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. dhlin@xmu.edu.cn.
authors.forename: Donghai
authors.initials: D
authors.lastname: Lin
authors.affiliation: High-field NMR Research Center, Key Laboratory of Chemical Biology of Fujian Province, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. xlliao@xmu.edu.cn.
authors.forename: Xinli
authors.initials: X
authors.lastname: Liao
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D009169:Mycobacterium tuberculosis; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D012269:Ribosomal Proteins / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C015494:ribosomal protein S1
keywords: NMR resonance assignments; RpsA; Trans-translation; Tuberculosis