title: Role of cytosolic calcium diffusion in cardiac purkinje cells.
abstract: The Cardiac Purkinje cells (PCs) exhibit distinct calcium (Ca2+) homeostasis than that in ventricular myocytes (VMs). Due to lack of t-tubules in PCs, the Ca2+ ions entering the cell have to diffuse through the cytoplasm to reach the sarcoplasmic reticulum (SR) before triggering Ca2+-induced-Ca2+-release (CICR). In recent experimental studies PCs have been shown to be more susceptible to action potential (AP) abnormalities than the VMs, however the exact mechanisms are poorly understood. In this study, we utilize morphologically realistic detailed biophysical mathematical model of a murine PC to systematically examine the role intracellular Ca2+ diffusion in the APs of PCs. A biphasic spatiotemporal Ca2+ diffusion process, as observed experimentally, was implemented in the model which includes radial Ca2+ wavelets and cell wide longitudinal Ca2+ diffusion wave (CWW). The AP morphology, specifically plateau, is affected due to changes in intracellular Ca2+ dynamics. When Ca2+ concentration in sarcolemmal region is elevated, it activated inward sodium Ca2+ exchanger (NCX) current resulting into prolongation of the plateau at faster diffusion rates. Our results demonstrate that the cytosolic Ca2+ diffusion waves play a significant role in shaping APs of PCs and could provide mechanistic insights into the increased arrhythmogeneity of PCs.
authors.forename: Bijay
authors.initials: B
authors.lastname: Limbu
authors.forename: Kushal
authors.initials: K
authors.lastname: Shah
authors.forename: Makarand
authors.initials: M
authors.lastname: Deo
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D055592:Biophysical Phenomena; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D004058:Diffusion; D006352:Heart Ventricles / Q000166:cytology; D051379:Mice; D008955:Models, Cardiovascular; D011689:Purkinje Cells / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism
chemical_list: D002118:Calcium