title: A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes.
abstract: Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around -100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Nishi-Tatsumi
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Yahagi
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takeuchi
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Toya
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Takarada
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Murayama
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Aita
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Sawada
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Piao
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Oya
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Shikama
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Masuda
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Kubota
authors.affiliation: Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Izumida
authors.affiliation: Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuzaka
authors.affiliation: Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Nakagawa
authors.affiliation: Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Sekiya
authors.affiliation: Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Iizuka
authors.affiliation: Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawakami
authors.affiliation: Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kadowaki
authors.affiliation: Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yamada
authors.affiliation: Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shimano
mesh_terms: D041721:3T3-L1 Cells; D000256:Adenoviridae / Q000235:genetics; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D024202:Electrophoretic Mobility Shift Assay; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism*; D005247:Feeding Behavior; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008099:Liver / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D020218:Response Elements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D023081:CCAAT-Binding Factor; C402244:nuclear factor Y; D008156:Luciferases; D064429:Fatty Acid Synthases
keywords: adipose tissue; fatty acid; transcription