title: Investigation of the humanH3.3B(H3F3B) gene expression as a novel marker in patients with colorectal cancer.
abstract: BACKGROUND
  H3.3 histone is a replacement histone subtype that is express in entire cell cycle phases and overexpress in transcriptionally active regions, promoter regions, and intergenic or intragenic regulatory elements. This histone encoded by two genes termedH3.3A(H3F3A) andH3.3B(H3F3B). Mutations of these two genes lead to some human cancers such as chondroblastoma, osteosarcoma, and epithelial ovarian cancer. The aims of this study were to quantitatively examine the expression ofH3.3Bgene in colorectal cancer (CRC) and to correlate their expression level with demographics and clinicopathological characteristics.
  
  
  METHODS
  We investigatedH3.3Bgene expression in CRC by relative quantitative real-time polymerase chain reaction (real-time PCR) technique for the first time. For this purpose, total RNA extracted, then cDNA synthesized andH3.3Bgene expression was evaluated with specific primers by real-time PCR in tumoral tissues and adjacent normal tissues of 36 patients with CRC, then statistical analysis was performed using SPSS software.
  
  
  RESULTS
  The results of this study indicated thatH3.3Bgene significantly overexpressed in tumoral tissue than adjacent normal tissue. Furthermore, statistical analysis represented the significant correlation between theH3.3Bgene expression and some of the clinicopathological characteristics.
  
  
  CONCLUSIONS
  Our study showed thatH3.3Bgene expression changes can be useful as a probable prognosis biomarker in the early stages of CRC before it metastasized.
authors.affiliation: Department of Medical Genetic, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors.forename: Habib Allah
authors.initials: HA
authors.lastname: Ayoubi
authors.affiliation: Department of Medical Genetic, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors.forename: Frouzandeh
authors.initials: F
authors.lastname: Mahjoubi
authors.affiliation: Department of General Surgery, Hazrat-e-Rasoul Hospital, Tehran University of Medical Sciences and Health Services, Tehran, Iran.
authors.forename: Rezvan
authors.initials: R
authors.lastname: Mirzaei
keywords: H3.3B gene; Histone; biomarker; colorectal cancer (CRC); real-time polymerase chain reaction (real-time PCR)