title: Role of nanoparticle size in self-assemble processes of collagen for tissue engineering application.
abstract: Nanoparticle mediated extracellular matrix may offer new and improved biomaterial to wound healing and tissue engineering applications. However, influence of nanoparticle size in extracellular matrix is still unclear. In this work, we synthesized different size of silver nanoparticles (AgNPs) comprising of 10nm, 35nm and 55nm using nutraceuticals (pectin) as reducing as well as stabilization agents through microwave irradiation method. Synthesized Ag-pectin nanoparticles were assimilated in the self-assemble process of collagen leading to fabricated collagen-Ag-pectin nanoparticle based scaffolds. Physico-chemical properties and biocompatibility of scaffolds were analyzed through FT-IR, SEM, DSC, mechanical strength analyzer, antibacterial activity and MTT assay. Our results suggested that 10nm sized Ag-pectin nanoparticles significantly increased the denaturation temperature (57.83°C) and mechanical strength (0.045MPa) in comparison with native collagen (50.29°C and 0.011MPa). The in vitro biocompatibility assay reveals that, collagen-Ag-pectin nanoparticle based scaffold provided higher antibacterial activity against to Gram positive and Gram negative as well as enhanced cell viability toward keratinocytes. This work opens up a possibility of employing the pectin caged silver nanoparticles to develop collagen-based nanoconstructs for biomedical applications.
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Vedhanayagam
authors.affiliation: Department of Chemistry, Amity School of Applied Sciences, Amity University, Gurgaon, India.
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Nidhin
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Natarajan
authors.initials: N
authors.lastname: Duraipandy
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Niranjan Dhanasekar
authors.initials: ND
authors.lastname: Naresh
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Jaganathan
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Ranganathan
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Manikantan Syamala
authors.initials: MS
authors.lastname: Kiran
authors.affiliation: Faculty of Allied Health Sciences, Chettinad Academy of Research & Education, Chennai 603 103, India.
authors.forename: Shoba
authors.initials: S
authors.lastname: Narayan
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
authors.forename: Balachandran Unni
authors.initials: BU
authors.lastname: Nair
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India. Electronic address: kjsreeram@clri.res.in.
authors.forename: Kalarical Janardhanan
authors.initials: KJ
authors.lastname: Sreeram
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D004355:Drug Stability; D006801:Humans; D055595:Mechanical Phenomena; D053768:Metal Nanoparticles / Q000737:chemistry*; D008872:Microwaves; D010316:Particle Size; D010368:Pectins / Q000737:chemistry; D055503:Protein Multimerization / Q000187:drug effects; D020836:Protein Structure, Quaternary; D012834:Silver / Q000737:chemistry*; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D012834:Silver; D010368:Pectins; D003094:Collagen
keywords: Collagen; Self-assembly; Silver-pectin; Thermal stability and compressive modulus