title: Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide.
abstract: Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte-specific switch-on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide-nucleic-acid-based linker. The ROS-sensing ability was assessed and validated in cell-free assays and in cell culture.
authors.affiliation: Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
authors.forename: Diana
authors.initials: D
authors.lastname: Andina
authors.affiliation: Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
authors.forename: Davide
authors.initials: D
authors.lastname: Brambilla
authors.affiliation: Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
authors.forename: Noah
authors.initials: N
authors.lastname: Munzinger
authors.affiliation: Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Frei
authors.affiliation: Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
authors.forename: Cristina
authors.initials: C
authors.lastname: Zivko
authors.affiliation: Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
authors.forename: Jean-Christophe
authors.identifier: http://orcid.org/0000-0001-5601-1292
authors.initials: JC
authors.lastname: Leroux
authors.affiliation: Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.|Biologisch-Pharmazeutische Fakultät, Institut für Pharmazie, Friedrich-Schiller-Universität Jena, 07743, Jena, Germany.
authors.forename: Paola
authors.initials: P
authors.lastname: Luciani
mesh_terms: D018938:Caco-2 Cells; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D017382:Reactive Oxygen Species / Q000032:analysis / Q000737:chemistry; D013481:Superoxides / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D013481:Superoxides
keywords: extracellular; fluorescent probe; peptide nucleic acid; ratiometric; reactive oxygen species