title: Intrinsic gene changes determine the successful establishment of stable renal cancer cell lines from tumor tissue.
abstract: Human tumor cell lines, especially those with complete data and follow-up, are important tools in tumor biological studies. Clear cell renal cell cancer (ccRCC) is not sensitive to radiotherapy or chemotherapy, and treatment of patients with distant metastasis relies on targeted therapy. Here, we report the establishment of seven new ccRCC stable cell lines that were continuously cultured for more than 20 generations among 81 cases of renal cell cancer. Moreover, gene expression and methylation in the established cell lines, in those that had a finite in vitro life span of less than 10 generations, and in cells that originated from the same culture at different generations were profiled using microarrays. Genes including SLC34A2, SEPP1, SULT1C4 and others were differentially expressed in established cell lines and finite cell lines, and changes in their expression might be caused by methylation or demethylation. The expression level of SLC34A2 was related not only to the life span in vitro culture but also to tumor size. Additionally, six of the seven new ccRCC cell lines had VHL deletions or termination mutations. So in addition to the establishment of seven new ccRCC cell lines with complete clinical data, we conclude that genes such as SLC34A2 and VHL play key roles in the continuous in vitro growth and development of ccRCC.
authors.affiliation: Cell Resource Center, Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing, China.
authors.forename: Hailiang
authors.identifier: 0000-0001-6587-1585
authors.initials: H
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Kidney Diseases, Department of Urology, Chinese People's Liberation Army Medical School, Chinese People's Liberation Army General Hospital, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Kidney Diseases, Department of Urology, Chinese People's Liberation Army Medical School, Chinese People's Liberation Army General Hospital, Beijing, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.affiliation: Cell Resource Center, Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Cell Resource Center, Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing, China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Kidney Diseases, Department of Urology, Chinese People's Liberation Army Medical School, Chinese People's Liberation Army General Hospital, Beijing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Cell Resource Center, Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008745:Methylation; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb / Q000235:genetics; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000235:genetics
chemical_list: C493557:SLC34A2 protein, human; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb; D051794:Von Hippel-Lindau Tumor Suppressor Protein
keywords: SLC34A2; VHL; clear cell renal cell carcinoma; stable cell lines