title: Starch materials as biocompatible supports and procedure for fast separation of macrophages.
abstract: Different starch derivatives were evaluated as supports for attachment and recovery of macrophages (RAW 264.7 line). Gelatinized starch (G-St), acetate starch (Ac-St), carboxymethyl starch and aminoethyl starch were synthesized and characterized by FTIR,1H NMR, SEM and static water contact angle. These polymers are filmogenic and may coat well the holder devices used for macrophage adhesion. They also present a susceptibility to mild hydrolysis with alpha-amylase, liberating the adhered macrophages. Cell counts, percentage of dead cells and level of tumor necrosis factor (TNF-α) were used to evaluate the possible interaction between macrophages and starch films. The high percentage of cell adhesion (90-95% on G-St and on Ac-St) associated with enzymatic detachment of macrophages from film-coated inserts, resulted in higher viabilities compared with those obtained with cells detached by current methods scrapping or vortex. This novel method allows a fast macrophage separation, with excellent yields and high viability of recovered cells.
authors.affiliation: Department of Chemistry and Biomed Center, Université du Québec à Montréal, C.P. 8888, Branch A, Montréal, Québec, H3C 3P8, Canada.
authors.forename: Khalil
authors.initials: K
authors.lastname: Sakeer
authors.affiliation: Department of Biological Sciences and Biomed Center, Université du Québec à Montréal, C.P. 8888, Branch A, Montréal, Québec H3C 3P8, Canada.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Scorza
authors.affiliation: Department of Biological Sciences and Biomed Center, Université du Québec à Montréal, C.P. 8888, Branch A, Montréal, Québec H3C 3P8, Canada.
authors.forename: Hugo
authors.initials: H
authors.lastname: Romero
authors.affiliation: Department of Chemistry and Biomed Center, Université du Québec à Montréal, C.P. 8888, Branch A, Montréal, Québec, H3C 3P8, Canada.
authors.forename: Pompilia
authors.initials: P
authors.lastname: Ispas-Szabo
authors.affiliation: Department of Chemistry and Biomed Center, Université du Québec à Montréal, C.P. 8888, Branch A, Montréal, Québec, H3C 3P8, Canada. Electronic address: mateescu.m-alexandru@uqam.ca.
authors.forename: Mircea Alexandru
authors.initials: MA
authors.lastname: Mateescu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002469:Cell Separation / Q000379:methods*; D006868:Hydrolysis; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D000067996:RAW 264.7 Cells; D013213:Starch / Q000737:chemistry*; D000516:alpha-Amylases
chemical_list: D001672:Biocompatible Materials; D013213:Starch; D000516:alpha-Amylases
keywords: Acetate starch; Alpha-amylase; Gelatinized starch; Macrophage separation; Starch derivatives; Tumor necrosis factor (TNF-α)