title: Dynamics of miRNA transcriptome during gonadal development of zebrafish.
abstract: Studies in non-teleost vertebrates have found microRNAs (miRNAs) to be essential for proper gonadal development. However, comparatively little is known about their role during gonadal development in teleost fishes. So far in zebrafish, a model teleost, transcript profiling throughout gonadal development has not been established because of a tiny size of an organ in juvenile stages and its poor distinguishability from surrounding tissues. We performed small RNA sequencing on isolated gonads of See-Thru-Gonad line, from the undifferentiated state at 3 weeks post fertilization (wpf) to fully mature adults at 24 wpf. We identified 520 gonadal mature miRNAs; 111 of them had significant changes in abundance over time, while 50 miRNAs were either testis- or ovary-enriched significantly in at least one developmental stage. We characterized patterns of miRNA abundance over time including isomiR variants. We identified putative germline versus gonadal somatic miRNAs through differential small RNA sequencing of isolated gametes versus the whole gonads. This report is the most comprehensive analysis of the miRNA repertoire in zebrafish gonads during the sexual development to date and provides an important database from which functional studies can be performed.
authors.affiliation: Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
authors.forename: Christopher
authors.initials: C
authors.lastname: Presslauer
authors.affiliation: Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
authors.forename: Teshome
authors.initials: T
authors.lastname: Tilahun Bizuayehu
authors.affiliation: Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
authors.forename: Martina
authors.initials: M
authors.lastname: Kopp
authors.affiliation: Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
authors.forename: Jorge M O
authors.initials: JM
authors.lastname: Fernandes
authors.affiliation: Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
authors.forename: Igor
authors.initials: I
authors.lastname: Babiak
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism; D006066:Gonads / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D010053:Ovary / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors; D059467:Transcriptome; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
chemical_list: D035683:MicroRNAs