title: Polydatin inhibits cell proliferation and induces apoptosis in laryngeal cancer and HeLa cells via suppression of the PDGF/AKT signaling pathway.
abstract: Polydatin (PD), a stilbene compound extracted from Polygonum cuspidatum, is suggested to possess anti-cancer activities, including inhibition of cell proliferation, cell cycle arrest, and induction of apoptosis. The platelet-derived growth factor (PDGF)/AKT signaling pathway plays complex roles in tumor suppression. However, the effect of PD on the PDGF/AKT signaling pathway in laryngeal cancer and HeLa cells has not been explored. MTT assay and flow cytometry showed that PD inhibited cell proliferation and induced apoptosis in Hep-2 and AMC-HN-8 cells. Western blot analysis indicated that PD inhibited the expression levels of PDGF-B and phosphorylated AKT (p-AKT) in both cells. Treatment of PDGF-B siRNA or PDGFR inhibitor found that after the PDGF signaling was inactivated, p-AKT expression was significantly decreased in Hep-2 cells. Tumor xenograft experiment in nude mice indicated PD significantly inhibited the growth of Hep-2 cells in vivo. In conclusion, PD inhibited cell proliferation and induced apoptosis in laryngeal cancer and HeLa cells via inactivation of the PDGF/AKT signaling pathway.
authors.affiliation: Department of Otolaryngology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
authors.forename: Baoyuan
authors.initials: B
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
authors.forename: Fengfang
authors.initials: F
authors.lastname: Yin
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D045468:Fallopia japonica / Q000737:chemistry; D005260:Female; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D005960:Glucosides; D010982:Platelet-Derived Growth Factor; D013267:Stilbenes; D051057:Proto-Oncogene Proteins c-akt; C058229:polydatin
keywords: HeLa cells; PDGF/AKT signaling pathway; apoptosis; cell proliferation; laryngeal cancer; polydatin