title: Folate-modified, indocyanine green-loaded lipid-polymer hybrid nanoparticles for targeted delivery of cisplatin.
abstract: Cisplatin is a potent antitumor drug, which is widely applied in clinical cancer treatment. However, cisplatin can hardly distinguish between healthy tissue and tumor tissue, resulting in serious toxic side effects. Indocyanine green (ICG) is a FDA-approved near-infrared (NIR) fluorescence dye which has been used in photothermal therapy and optically mediated diagnostic, but the application of ICG is limited by its concentration-dependent aggregation, poor aqueous stability in vitro, lack of target specificity and rapid elimination from the body. Herein, to overcome these limitations of cisplatin and ICG, we fabricated folate-modified, cisplatin, ICG-loaded lipid-polymer hybrid nanoparticles (FCINPs) using a single-step sonication method. The FCINPs exhibited well-defined monodispersity, significant stability and excellent NIR penetration ability. The intracellular uptake experiment showed that the targeting efficacy of the FCINPs was more effective in folate receptors (FRs) over-expressing MCF-7 cells than FRs negative A549 cells. In addition, compared with chemo or photothermal treatment alone, the treatment of FCINPs in combination with 808 nm NIR laser irradiation can significantly induce the apoptosis and necrosis of MCF-7 cells. These findings indicated that the FCINPs would be a promising nanosized drug formulation for tumor-targeted therapy in the future.
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.
authors.forename: Lianshuai
authors.initials: L
authors.lastname: Gu
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Shi
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.|b School of Pharmacy , Wannan Medical College , Wuhu , China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: You
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Wang
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.
authors.forename: Gaoju
authors.initials: G
authors.lastname: Wen
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.affiliation: c Laboratory of Organic Chemistry , ETH Zurich , Zurich , Switzerland.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: d Huadong Medical Institute of Biotechniques , Nanjing , China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , China.
authors.forename: Baiwang
authors.initials: B
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D002945:Cisplatin / Q000737:chemistry* / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D010713:Phosphatidylcholines; D011108:Polymers; D005492:Folic Acid; D007208:Indocyanine Green; D002945:Cisplatin
keywords: Cisplatin; folate; indocyanine green; lipid-polymer hybrid nanoparticles; photothermal therapy