title: Anti-ageing properties of Khelma Longevity™: treatment of human fibroblasts increases proteasome levels and decreases the levels of oxidized proteins.
abstract: We have determined the putative anti-ageing properties of Khelma Longevity™, a formula based on various natural compounds from the Mediterranean area. Human primary fibroblast cultures were treated with a wide range of concentrations of Khelma Longevity™ for 1 day or 3 consecutive days. Following these treatments, two major and complementary biomarkers of ageing were measured, namely, the proteasome and the amount of oxidized proteins. It was observed that 24h of treatment with Khelma Longevity™ resulted in a maximum increase of about 41% of the total protein levels of 20S proteasome. Levels of oxidized proteins were reduced by almost 6.5-fold following longer treatments. Specifically we have observed a maximum decrease of protein carbonyls to 84.7% in comparison with nontreated control cells following 3 days of continuous treatment with Khelma Longevity™. These results support the notion that formulas rich in natural compounds from the Mediterranean area possess anti-ageing properties.
authors.affiliation: National Hellenic Research Foundation, Institute of Biology, Medicinal Chemistry and Biotechnology, 48 Vassileos Constantinou Ave., 11635 Athens, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Voutetakis
authors.affiliation: National Hellenic Research Foundation, Institute of Biology, Medicinal Chemistry and Biotechnology, 48 Vassileos Constantinou Ave., 11635 Athens, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Delitsikou
authors.affiliation: Khelma Hellas S.A., Fidiou 11, 10678 Athens, Greece.
authors.forename: Michel Georges
authors.initials: MG
authors.lastname: Magouritsas
authors.affiliation: National Hellenic Research Foundation, Institute of Biology, Medicinal Chemistry and Biotechnology, 48 Vassileos Constantinou Ave., 11635 Athens, Greece. Electronic address: sgonos@eie.gr.
authors.forename: Efstathios S
authors.initials: ES
authors.lastname: Gonos
mesh_terms: D001688:Biological Products / Q000494:pharmacology*; D016922:Cellular Senescence / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D050050:Protein Carbonylation / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*
chemical_list: D001688:Biological Products; D011506:Proteins; D046988:Proteasome Endopeptidase Complex