title: Enhanced ethanol production at commercial scale from molasses using high gravity technology by mutant S. cerevisiae.
abstract: Very high gravity (VHG) technology was employed on industrial scale to produce ethanol from molasses (fermented) as well as by-products formation estimation. The effect of different Brix° (32, 36 and 40) air-flow rates (0.00, 0.20, 0.40, and 0.60vvm) was studied on ethanol production. The maximum ethanol production was recorded to be 12.2% (v/v) at 40 Brix° with 0.2vvm air-flow rate. At optimum level aeration and 40 Brix° VHG, the residual sugar level was recorded in the range of 12.5-18.5g/L, whereas the viable cell count remained constant up to 50h of fermentation and dry matter production increased with fermentation time. Both water and steam consumption reduced significantly under optimum conditions of Brix° and aeration rate with compromising the ethanol production. Results revealed VHG with continuous air flow is viable technique to reduce the ethanol production cost form molasses at commercial scale.
authors.affiliation: Department of Basic Sciences, Biochemistry Section, College of Veterinary and Animal Sciences, Jhang Campus 35200, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arshad
authors.affiliation: Department of Basic Sciences, Biochemistry Section, College of Veterinary and Animal Sciences, Jhang Campus 35200, Pakistan.
authors.forename: Tariq
authors.initials: T
authors.lastname: Hussain
authors.affiliation: The University of Lahore, Department of Chemistry, Lahore, Pakistan. Electronic address: bosalvee@yahoo.com.
authors.forename: Munawar
authors.initials: M
authors.lastname: Iqbal
authors.affiliation: The University of Lahore, Institute of Molecular Biology and Biotechnology, Lahore, Pakistan.
authors.forename: Mazhar
authors.initials: M
authors.lastname: Abbas
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D018471:Hypergravity; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D008965:Molasses / Q000032:analysis / Q000191:economics / Q000382:microbiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D000431:Ethanol
keywords: Aeration; Brix; Ethanol; Molasses; Very high gravity technology