title: Human eyelid adipose tissue-derived Schwann cells promote regeneration of a transected sciatic nerve.
abstract: Schwann cells (SCs) can promote the regeneration of injured peripheral nerves while the clinical application is limited by donor site complications and the inability to generate an ample amount of cells. In this study, we have isolated human eyelid adipose-derived Schwann cells (hE-SCs) from human eyelid adipose tissue and identified the cell phenotype and function. Using immunofluorescence and H &E staining, we detected subtle nerve fibers and SCs in human eyelid adipose tissue. Immunofluorescence staining indicated that hE-SCs expressed glial markers, such as S100, p75NTR GFAP, Sox10 and Krox20. To explore whether hE-SCs promote the regeneration of injured peripheral nerves in vivo, a Balb/c-nu mice model was used in the study, and mice were randomly assigned to five groups: Matrigel; hE-SCs/P0; hE-SCs/P2; hE-FLCs/P2; and Autograft. After 12 weeks, functional and histological assessments of the regenerated nerves showed that sciatic nerve defect was more effectively repaired in the hE-SCs/P2 group which achieved 66.1 ± 6.5% purity, than the other three groups and recovered to similar level to the Autograft group. These results indicated that hE-SCs can promote the regeneration of injured peripheral nerve and the abundant, easily accessible supply of adipose tissue might be a promising source of SCs for peripheral nerve repair.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Gangyang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Rehabilitation Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Department of Orthopaedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Plastic Surgery Hospital, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, People's Republic of China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Plastic and Reconstructive Surgery, Lanzhou Maternal and Child Health Care Hospital, Lanzhou, Gansu, People's Republic of China.
authors.forename: Lina
authors.initials: L
authors.lastname: Niu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Plastic and Reconstructive Surgery, Lanzhou Maternal and Child Health Care Hospital, Lanzhou, Gansu, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Zunli
authors.initials: Z
authors.lastname: Shen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D017690:Cell Transplantation; D004195:Disease Models, Animal; D005143:Eyelids / Q000166:cytology*; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D012038:Regeneration; D012583:Schwann Cells / Q000737:chemistry / Q000502:physiology*; D012584:Sciatic Nerve / Q000293:injuries*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers