title: Cobalt (II) complex with novel unsymmetrical tetradentate Schiff base (ON) ligand: in vitro cytotoxicity studies of complex, interaction with DNA/protein, molecular docking studies, and antibacterial activity.
abstract: [C20H17N3O2] and cobalt (II) complex [Co(L2)(MeOH)2].ClO4, (L2= 4-((E)-1-((2-(((E)-pyridin-2-ylmethylene) amino) phenyl) imino) ethyl) benzene-1, 3-diol) novel Schiff base has been synthesiszed and chracterized by Fourier transform infrared, UV-vis,1H-NMR spectroscopy, and elemental analysis techniques. The interaction of Co(II) complex with DNA and BSA was investigated by electronic absorption spectroscopy, fluorescence spectroscopy, circular dichroism, and thermal denaturation studies. Our experiments indicate that this complex could strongly bind to CT-DNA via minor groove mechanism. In addition, fluorescence spectrometry of BSA with the complex showed that the fluorescence quenching mechanism of BSA was of static type. The complex exhibited significant in vitro cytotoxicity against three human cancer cell lines (JURKAT, SKOV3, and U87). The molecular docking experiment effectively proved the binding of complex to DNA and BSA. Finally, antibacterial assay over gram-positive and gram-negative pathogenic bacterial strains was studied.
authors.affiliation: a Department of Chemistry , Isfahan University of Technology , Isfahan 84156-83111 , Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Shokohi-Pour
authors.affiliation: a Department of Chemistry , Isfahan University of Technology , Isfahan 84156-83111 , Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Chiniforoshan
authors.affiliation: b Department of Agronomy and Plant Breeding , College of Agriculture, Isfahan University of Technology , Isfahan 84156-83111 , Iran.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Sabzalian
authors.affiliation: c Student Research Committee, Department of Immunology and Allergy , Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Seyed-Alireza
authors.initials: SA
authors.lastname: Esmaeili
authors.affiliation: d Student Research Committee, Department of Medical Biotechnology , Nanotechnology Research Center, School of Medicine, Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Amir Abbas
authors.initials: AA
authors.lastname: Momtazi-Borojeni
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002942:Circular Dichroism; D003035:Cobalt / Q000737:chemistry* / Q000494:pharmacology; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D006801:Humans; D019169:Jurkat Cells; D008024:Ligands; D062105:Molecular Docking Simulation; D009369:Neoplasms / Q000188:drug therapy*; D012545:Schiff Bases / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D056831:Coordination Complexes; D008024:Ligands; D012545:Schiff Bases; D012710:Serum Albumin, Bovine; D003035:Cobalt; D003300:Copper
keywords: Co(II) complex; antibacterial activity; binding with DNA/BSA; cytotoxic activity; unsymmetrical Schiff base