title: Microfluidic Electroporation-Facilitated Synthesis of Erythrocyte Membrane-Coated Magnetic Nanoparticles for Enhanced Imaging-Guided Cancer Therapy.
abstract: Biomimetic cell membrane-coated nanoparticles (CM-NPs) with superior biochemical properties have been broadly utilized for various biomedical applications. Currently, researchers primarily focus on using ultrasonic treatment and mechanical extrusion to improve the synthesis of CM-NPs. In this work, we demonstrate that microfluidic electroporation can effectively facilitate the synthesis of CM-NPs. To test it, Fe3O4magnetic nanoparticles (MNs) and red blood cell membrane-derived vesicles (RBC-vesicles) are infused into a microfluidic device. When the mixture of MNs and RBC-vesicles flow through the electroporation zone, the electric pulses can effectively promote the entry of MNs into RBC-vesicles. After that, the resulting RBC membrane-capped MNs (RBC-MNs) are collected from the chip and injected into experimental animals to test the in vivo performance. Owing to the superior magnetic and photothermal properties of the MN cores and the long blood circulation characteristic of the RBC membrane shells, core-shell RBC-MNs were used for enhanced tumor magnetic resonance imaging (MRI) and photothermal therapy (PTT). Due to the completer cell membrane coating, RBC-MNs prepared by microfluidic electroporation strategy exhibit significantly better treatment effect than the one fabricated by conventional extrusion. We believe the combination of microfluidic electroporation and CM-NPs provides an insight into the synthesis of bioinpired nanoparticles to improve cancer diagnosis and therapy.
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Rao
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cai
authors.affiliation: Department of Oral Maxillofacial Head Neck Oncology, School and Hospital of Stomatology, Wuhan University , Wuhan, Hubei 430079, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Bu
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Qing-Quan
authors.initials: QQ
authors.lastname: Liao
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Shi-Shang
authors.initials: SS
authors.lastname: Guo
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Xing-Zhong
authors.initials: XZ
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences , Suzhou, Jiangsu 215163, China.
authors.forename: Wen-Fei
authors.initials: WF
authors.lastname: Dong
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Wei
authors.identifier: 0000-0003-4789-362X
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D018274:Electroporation; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000188:drug therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046210:Microfluidic Analytical Techniques; D010316:Particle Size; D010789:Phototherapy; D000067996:RAW 264.7 Cells; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D058185:Magnetite Nanoparticles
keywords: cancer photothermal therapy; iron oxide magnetic nanoparticles; microfluidic electroporation; red blood cell membrane; tumor magnetic resonance imaging