title: Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L.
abstract: Present study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of the Monarda citriodora Cerv. ex Lag. (Lamiaceae/Labiatae). Anticancer and antimicrobial potential of isolated endophytes have also been investigated.
  
  
  
  A total of twenty eight fungal endophytes belonging to 11 different genera were isolated from this plant. All the endophytic fungi belonged to the Ascomycota phylum. The leaves were immensely rich in fungal species, while roots showed the highest tissue specific fungal dominance. Out of 28 fungal species, 72% endophytic extracts were found cytotoxic against one or more human cancer cell lines. The most prominent anticancer activity (IC50value <10 μg/mL) was shown by MC-14 L (Fusarium oxysporum), MC-14 F (F. oxysporum), MC-18 L (Aspergillus fumigatus), MC-24 L (Cladosporium tenuissimum), MC-25 L (Fusarium sp.), MC-26 F (F. oxysporum) extracts. 75% of the extracts showed antimicrobial activities in agar disc-diffusion assay and 27% in the tube dilution method (MIC <100 μg/mL) respectively against the tested pathogens. Extracts of MC-14 L (F. oxysporum) and MC-18 L (A. fumigatus) displayed broad spectrum antimicrobial activity.
  
  
  
  These results indicated that M. citriodora harbors a rich fungal endophytic community with anticancer and antimicrobial activities. The isolated endophyte MC-24 L (C. tenuissimum) has the potential to be a source of novel cytotoxic/antimicrobial compounds. This is the first report of diversity of fungal endophytes isolated from M. citriodora.
authors.affiliation: Microbial Biotechnology Division, Indian Institute of Integrative Medicine, Jammu, India. meenusamiksha@rediffmail.com.
authors.forename: Meenu
authors.initials: M
authors.lastname: Katoch
authors.affiliation: Microbial Biotechnology Division, Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Shipra
authors.initials: S
authors.lastname: Phull
authors.affiliation: Cancer Pharmacology Divison, Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Shagun
authors.initials: S
authors.lastname: Vaid
authors.affiliation: Cancer Pharmacology Divison, Indian Institute of Integrative Medicine, Jammu, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Singh
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000145:classification / Q000378:metabolism; D001232:Aspergillus fumigatus / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D044822:Biodiversity; D045744:Cell Line, Tumor / Q000187:drug effects; D002963:Cladosporium / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005658:Fungi / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D005670:Fusarium / Q000378:metabolism; D045325:HCT116 Cells / Q000187:drug effects; D006801:Humans; D007194:India; D061986:MCF-7 Cells / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D031338:Monarda / Q000382:microbiology*; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D004271:DNA, Fungal
keywords: Antimicrobial activity; Cytotoxicity; Endophytic fungi; MTT assay; Monarda citriodora; Tube dilution method