title: [Glycyrrhizinic acid modulates the immunity of MRL/lpr mice and related mechanism].
abstract: Objective To investigate the immunological effect of glycyrrhizic acid (GA) on MRL/lpr mice and its underlying mechanism. Methods The research included 10 wild type C57BL/6 mice and 40 MRL/lpr mice. MRL/lpr mice were randomly assigned to four groups: MRL/lpr control, MRL/lpr treated with dexamethasone (1.5 mg/kg), MRL/lpr treated with GA (20 mg/kg) and GA (40 mg/kg), 10 mice in each group. The serum levels of interleukin 4 (IL-4) and interferon-γ (IFN-γ) were tested by ELISA. The ratio of Th1/Th2 cell subsets in spleen was analyzed by flow cytometry. The protein levels of GATA-binding protein 3 (GATA3), T-bet, p-JAK3, JAK3, phosphorylated signal transducer and activator of transcription 3 (p-STAT3), STAT3, p-NF-κBp65, NF-κBp65, p-IκBα, IκBα in spleen were detected by Western blot analysis. Results GA decreased serum levels of IL-4 and increased IFN-γ, modulated the balance of Th1/Th2 cell subsets in spleen markedly. GA inhibited the protein levels of GATA3, p-JAK3, p-STAT3, p-NF-κB, p-IκBα and increased T-bet protein in MRL/lpr mice. Conclusion Administration of GA can ameliorate the immune function in MRL/lpr mice.
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China.
authors.forename: Jixia
authors.initials: J
authors.lastname: Chai
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China.
authors.forename: Meiqun
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Zou
authors.affiliation: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Rheumatology and Immunology, First Affiliated Hospital, Bengbu Medical College, Bengbu 233004, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Rheumatology and Immunology, First Affiliated Hospital, Bengbu Medical College, Bengbu 233004, China. *Corresponding author, E-mail: uglboy2002@126.com.
authors.forename: Changhao
authors.initials: C
authors.lastname: Xie
mesh_terms: D000818:Animals; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000276:immunology; D019695:Glycyrrhizic Acid / Q000008:administration & dosage*; D006801:Humans; D007109:Immunity / Q000187:drug effects*; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology
chemical_list: D050990:GATA3 Transcription Factor; D016328:NF-kappa B; D019695:Glycyrrhizic Acid; D007371:Interferon-gamma