title: Effect of high fat diets on the NTPDase, 5'-nucleotidase and acetylcholinesterase activities in the central nervous system.
abstract: High fat diets are associated with the promotion of neurological diseases, such as Alzheimer disease (AD). This study aim investigate the high fat diets role to promotion of AD using as biochemistry parameter of status of central nervous system through the NTPDase, 5'-nucleotidase and acetylcholinesterase (AChE) activities in brain of young rats. The intake of high fat diets promotes an inhibition of purinergic and cholinergic functions, mainly in the long-term exposure to saturated and saturated/unsaturated diets. The AChE activity was decreased to supernatant and synaptosomes tissues preparations obtained from cerebral cortex in average of 20%, to both groups exposed to saturated and saturated/unsaturated diets, when compared to the control group. Very similar results were found in hippocampus and cerebellum brain areas. At same time, the adenine nucleotides hydrolysis in synaptosomes of cerebral cortex were decreased to ATP, ADP and AMP after the long-term exposure to high fat diets, as saturated and saturated/unsaturated. The inhibition of ATP hydrolysis was of 26% and 39% to saturated and saturated/unsaturated diets, respectively. ADP hydrolysis was decreased in 20% to saturated diet, and AMP hydrolysis was decreased in 25% and 33% to saturated and saturated/unsaturated diets, respectively, all in comparison to the control. Thus, we can suggest that the effects of high diets on the purinergic and cholinergic nervous system may contribute to accelerate the progressive memory loss, to decline in language and other cognitive disruptions, such as AD patients presents.
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, Campus Sertão, Rodovia ERS 135 km 25, Distrito Engenheiro Luiz Englert, 99170-000, Sertão, RS, Brazil; Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal da Fronteira Sul, Campus Erechim, Rodovia ERS 135 km 72, n° 200, 99700-970, Erechim, RS, Brazil. Electronic address: rosikaizer@gmail.com.
authors.forename: Rosilene Rodrigues
authors.initials: RR
authors.lastname: Kaizer
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima, 97105-900. Santa Maria, RS, Brazil.
authors.forename: Rosélia Maria
authors.initials: RM
authors.lastname: Spanevello
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima, 97105-900. Santa Maria, RS, Brazil.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Costa
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima, 97105-900. Santa Maria, RS, Brazil.
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima, 97105-900. Santa Maria, RS, Brazil.
authors.forename: Maria Rosa Chitolina
authors.initials: MRC
authors.lastname: Schetinger
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D059305:Diet, High-Fat; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*
chemical_list: D000110:Acetylcholinesterase; D015720:5'-Nucleotidase
keywords: Adenine nucleotides; Alzheimer disease; Cholinergic; Hydrolysis; Purinergic