title: Rapid typing of Mannheimia haemolytica major genotypes 1 and 2 using MALDI-TOF mass spectrometry.
abstract: Genotype 2M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly differentiate genotype 2 strains in veterinary diagnostic laboratories.
authors.affiliation: University of Nebraska-Lincoln, Institute of Agriculture and Natural Resources, School of Veterinary Medicine and Biomedical Sciences, Lincoln, NE, USA. Electronic address: jdloy@unl.edu.
authors.forename: John Dustin
authors.initials: JD
authors.lastname: Loy
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE, USA. Electronic address: mike.clawson@ars.usda.gov.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Clawson
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D019450:Genes, MDR / Q000235:genetics; D005838:Genotype; D008168:Lung / Q000382:microbiology; D016978:Mannheimia haemolytica / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D016871:Pasteurellaceae Infections / Q000175:diagnosis / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial
keywords: Bovine respiratory disease; Genotypes; MALDI-TOF mass spectrometry; Mannheimia haemolytica