title: High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae).
abstract: Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the LepidopteraSpodoptera exiguawere produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ and Gram- as well as their carbohydrate binding specificities was determined for the three lectins. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as a biosensor or for other biotechnological applications involving bacterial capture.
authors.affiliation: ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain. gasmi.leila@gmail.com.
authors.forename: Leila
authors.initials: L
authors.lastname: Gasmi
authors.affiliation: ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain. ferrej@uv.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Ferré
authors.affiliation: ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain. sherrero@uv.es.
authors.forename: Salvador
authors.initials: S
authors.lastname: Herrero
mesh_terms: D000371:Agglutination; D000372:Agglutination Tests; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D004926:Escherichia coli; D007113:Immunity, Innate; D019476:Insect Proteins / Q000737:chemistry / Q000502:physiology*; D037181:Lectins, C-Type / Q000737:chemistry / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D018411:Spodoptera / Q000276:immunology* / Q000382:microbiology
chemical_list: D019476:Insect Proteins; D037181:Lectins, C-Type
keywords: C-type lectin; CRD; E. coli; agglutination; bacterial detection