title: Treated wastewater phytotoxicity assessment using Lactuca sativa: Focus on germination and root elongation test parameters.
abstract: Sensitive and simple ecotoxicological bioassays like seed germination and root elongation tests are commonly used to evaluate the phytotoxicity of waste and industrial discharge waters. Although the tests are performed following national and international standards, various parameters such as the number of seeds per dish, the test duration or the type of support used remain variable. To be able to make a correct comparison of results from different studies, it is crucial to know which parameter(s) could affect ecotoxicological diagnosis. We tested four different control waters and three seed densities. No significant differences on either germination rate or root elongation endpoints were shown. Nevertheless, we found that the four lettuce cultivars (Appia, batavia dorée de printemps, grosse blonde paresseuse, and Kinemontepas) showed significantly different responses when watered with the same and different metal-loaded industrial discharge water. From the comparison, it is clear that a differential sensitivity scale occurs among not just species but cultivars.
authors.affiliation: UMR 6249, Chrono-Environment, University of Franche-Comté/CNRS, 16, route de Gray, 25000 Besançon, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Priac
authors.affiliation: UMR 6249, Chrono-Environment, University of Franche-Comté/CNRS, 16, route de Gray, 25000 Besançon, France.
authors.forename: Pierre-Marie
authors.initials: PM
authors.lastname: Badot
authors.affiliation: UMR 6249, Chrono-Environment, University of Franche-Comté/CNRS, 16, route de Gray, 25000 Besançon, France. Electronic address: gregorio.crini@univ-fcomte.fr.
authors.forename: Grégorio
authors.initials: G
authors.lastname: Crini
mesh_terms: D054750:Ecotoxicology / Q000379:methods; D018525:Germination / Q000187:drug effects; D018545:Lactuca / Q000187:drug effects*; D018517:Plant Roots / Q000187:drug effects; D018675:Toxicity Tests; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bio-essais; Bioassay; Cultivar; Discharge water; Lactuca sativa; Rejet; Sensitivity scale; Variété; Échelle de sensibilité