title: Downregulation of miR-377 Promotes Oral Squamous Cell Carcinoma Growth and Migration by Targeting HDAC9.
abstract: microRNAs are the post-transcriptional regulators implicated in the initiation and progression of various cancer types, including oral squamous cell carcinoma (OSCC). Here, we investigated the role of miR-377 in OSCC tumorigenesis. miR-377 expression was reduced in OSCC samples and cell line (UPCI-SCC-116), and was associated with patient survival. In vitro restoration of miR-377 repressed cell growth, induced apoptosis, and reduced cell migration. We identified HDAC9 as a target of miR-377 and found miR-377 to regulate HDAC9 and its pro-apoptotic target, NR4A1/Nur77. Our findings show that miR-377 targets HDAC9 pathway in OSCC, suggesting that miR-377-HDAC9 axis may provide a novel therapeutic target for OSCC therapy.
authors.affiliation: a Department of Otolaryngology and Head and Neck Surgery , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Bhawna
authors.initials: B
authors.lastname: Rastogi
authors.affiliation: b Department of Experimental Medicine and Biotechnology , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: b Department of Experimental Medicine and Biotechnology , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Raut
authors.affiliation: a Department of Otolaryngology and Head and Neck Surgery , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Naresh K
authors.initials: NK
authors.lastname: Panda
authors.affiliation: c Department of Oral Health Sciences Centre , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Vidya
authors.initials: V
authors.lastname: Rattan
authors.affiliation: b Department of Experimental Medicine and Biotechnology , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Nainesh
authors.initials: N
authors.lastname: Joshi
authors.affiliation: b Department of Experimental Medicine and Biotechnology , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Madhu
authors.initials: M
authors.lastname: Khullar
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D004794:Enzyme Repression; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009062:Mouth Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012097:Repressor Proteins
chemical_list: D020413:3' Untranslated Regions; C534063:MIRN377 microRNA, human; D035683:MicroRNAs; C539821:NR4A1 protein, human; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D012097:Repressor Proteins; C486544:HDAC9 protein, human; D006655:Histone Deacetylases
keywords: Histone deacetylase 9; Oral squamous cell carcinoma; microRNA-377