title: Association of serum lipids and coronary artery disease with polymorphisms in the apolipoprotein AI-CIII-AIV gene cluster.
abstract: Genetic variants are considered as one of the main determinants of the concentration of serum lipids and coronary artery disease (CAD). Polymorphisms in the Apolipoprotein (Apo) AI-CIII-AIV gene cluster has been known to affect the concentrations of various lipid sub-fractions and the risk of CAD. The present study assessed associations between polymorphisms of the Apo AI-CIII-AIV gene cluster, [ApoA-I,-75G > A, (rs1799837); ApoC-III 3238C > G, (SstI), (rs5128) and ApoA-IV, Thr347Ser(347A > T), (rs675)] with serum lipids and their contributions to CAD in North Indian population. We recruited age, sex matched, 200 CAD patients and 200 healthy controls and tested them for fasting levels of serum lipids. We genotyped selected polymorphisms using polymerase chain reaction-restriction fragment length polymorphism. There were no statistically significant association of selected polymorphisms (or their combinations) with CAD even after employing additive, dominant and recessive models. However there was significant association of selected polymorphisms with various lipid traits amongst the control cohort (p< 0.05). Mean levels of high density lipoprotein cholesterol and triglycerides were found to be significantly higher among controls carrying at least one mutant allele at ApoA1-75G > A (p= 0.019) and ApoCIIISstI(p< 0.001) polymorphism respectively. Our study observed that the selected polymorphisms in the ApoAI-CIII-AIV gene cluster although significantly affect various lipid traits but this affect does not seem to translate into association with CAD, at least among North Indian population.
authors.affiliation: Department of Cardiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences , Lucknow , UP , India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Rai
authors.affiliation: Department of Cardiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, UP, India; Department of Cardiology, Sahara India Medical Institute, Lucknow, UP, India.
authors.forename: Nakul
authors.initials: N
authors.lastname: Sinha
authors.affiliation: Human Genomics Laboratory, School of Sport Exercise and Health Sciences, Loughborough University , Loughborough LE11 3TU , UK.
authors.forename: James
authors.initials: J
authors.lastname: Finn
authors.affiliation: Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences , Lucknow , UP , India.
authors.forename: Suraksha
authors.initials: S
authors.lastname: Agrawal
authors.affiliation: Human Genomics Laboratory, School of Sport Exercise and Health Sciences, Loughborough University , Loughborough LE11 3TU , UK.
authors.forename: Sarabjit
authors.initials: S
authors.lastname: Mastana
keywords: apolipoprotein (Apo) AI-CIII-AIV gene cluster; coronary artery disease (CAD); linkage disequilibrium, haplotypes; serum lipids; single nucleotide polymorphisms (SNPs)