title: bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle.
abstract: Intramuscular fat deposition or marbling is essential for high quality beef. The molecular mechanism of adipogenesis in skeletal muscle remains largely unknown. In this study, we isolated Platelet-derived growth factor receptor α (PDGFRα) positive progenitor cells from fetal bovine skeletal muscle and induced into adipocytes. Using miRNAome sequencing, we revealed that bta-miR-23a was an adipogenic miRNA mediating bovine adipogenesis in skeletal muscle. The expression of bta-miR-23a was down-regulated during differentiation of PDGFRα+progenitor cells. Forced expression of bta-miR-23a mimics reduced lipid accumulation and inhibited the key adipogenic transcription factor peroxisome proliferative activated receptor gamma (PPARγ) and CCAAT/enhancer binding protein alpha (C/EBPα). Whereas down-regulation of bta-miR-23a by its inhibitors increased lipid accumulation and expression of C/EBPα, PPARγ and fatty acid-binding protein 4 (FABP4). Target prediction analysis revealed that ZNF423 was a potential target of bta-miR-23a. Dual-luciferase reporter assay revealed that bta-miR-23a directly targeted the 3'-UTR of ZNF423. Together, our data showed that bta-miR-23a orchestrates early intramuscular adipogeneic commitment as an anti-adipogenic regulator which acts by targeting ZNF423.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Long
authors.initials: L
authors.lastname: Guan
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yishen
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Zhengkui
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Guangxi 530004, China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
authors.forename: Qiyuan
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Animal Husbandry Station of Wulagai, Wulagai 026321, China.
authors.forename: Shengyun
authors.initials: S
authors.lastname: Jin
authors.affiliation: Animal Husbandry Station of Wulagai, Wulagai 026321, China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Bao
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Huijiang
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Du
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Junya
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Lupei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics*; D002469:Cell Separation; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence; D005333:Fetus / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D035683:MicroRNAs / Q000235:genetics*; D018482:Muscle, Skeletal / Q000166:cytology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D020796:Receptor, Platelet-Derived Growth Factor alpha