title: Evaluation of bioactive glass incorporated poly(caprolactone)-poly(vinyl alcohol) matrix and the effect of BMP-2 modification.
abstract: Composite materials having mechanical and biological properties similar to those of human bones are needed for bone regeneration and repair. In the present study, composites were made by incorporating bioactive glass (BG) into polycaprolactone (PCL)-polyvinyl alcohol (PVA) (PCLPVA) matrix. Composites with different BG contents of 10, 25 and 50wt% were fabricated by an in-situ blending method. Physicochemical properties measurements found that the composite with 50wt% BG in the PCLPVA organic matrix exhibited the best mechanical properties (compressive strength and compressive young's modulus up to 32.26MPa and 530.91MPa, respectively). We investigated the effects of the BG content on cell adhesion, proliferation and osteogenic activity of UMR-106 cells grown on the scaffolds using in vitro cell culture assay. The composite scaffolds having 25wt% BG showed a significant increase in their cell adhesion capability and a faster cell proliferation. They also exhibited cell adhesion and spreading morphology after only 5days of culturing. For these reasons, we chose to attach the bone morphogenetic protein (BMP)-2 to this composite. The resulting composite (labeled BMP-2-loaded PCLPVABG25) showed significant improvement in the UMR-106 cells adhesion, in the enhancement in osteogenic differentiation and osteoinductivity of this composite.
authors.affiliation: Department of Physics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Khamsone
authors.initials: K
authors.lastname: Keothongkham
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok 10900, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10900, Thailand; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
authors.forename: Narattaphol
authors.initials: N
authors.lastname: Charoenphandhu
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok 10900, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10900, Thailand.
authors.forename: Jirawan
authors.initials: J
authors.lastname: Thongbunchoo
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok 10900, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10900, Thailand.
authors.forename: Panan
authors.initials: P
authors.lastname: Suntornsaratoon
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok 10900, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10900, Thailand.
authors.forename: Nateetip
authors.initials: N
authors.lastname: Krishnamra
authors.affiliation: Department of Materials Science Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Tang
authors.affiliation: Department of Physics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. Electronic address: fsciwpp@ku.ac.th.
authors.forename: Weeraphat
authors.initials: W
authors.lastname: Pon-On
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry* / Q000494:pharmacology; D055119:Elastic Modulus; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D055396:Bone Morphogenetic Protein 2; D011091:Polyesters; C016240:polycaprolactone; D011142:Polyvinyl Alcohol
keywords: Bioactive glass; Bone morphogenetic protein (BMP); Composite porous scaffold; Mechanical properties; PCL; Poly(vinyl alcohol)