title: Assessment of Reproducibility of Laser Electrospray Mass Spectrometry using Electrospray Deposition of Analyte.
abstract: A nonresonant, femtosecond (fs) laser is employed to desorb samples of Victoria blue deposited on stainless steel or indium tin oxide (ITO) slides using either electrospray deposition (ESD) or dried droplet deposition. The use of ESD resulted in uniform films of Victoria blue whereas the dried droplet method resulted in the formation of a ring pattern of the dye. Laser electrospray mass spectrometry (LEMS) measurements of the ESD-prepared films on either substrate were similar and revealed lower average relative standard deviations for measurements within-film (20.9%) and between-films (8.7%) in comparison to dried droplet (75.5% and 40.2%, respectively). The mass spectral response for ESD samples on both substrates was linear (R2> 0.99), enabling quantitative measurements over the selected range of 7.0 × 10-11to 2.8 × 10-9mol, as opposed to the dried droplet samples where quantitation was not possible (R2= 0.56). The limit of detection was measured to be 210 fmol. Graphical Abstract ᅟ.
authors.affiliation: Department of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
authors.forename: Habiballah
authors.initials: H
authors.lastname: Sistani
authors.affiliation: Department of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
authors.forename: Santosh
authors.initials: S
authors.lastname: Karki
authors.affiliation: Department of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
authors.forename: Jieutonne J
authors.initials: JJ
authors.lastname: Archer
authors.affiliation: Department of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
authors.forename: Fengjian
authors.initials: F
authors.lastname: Shi
authors.affiliation: Department of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA. rjlevis@temple.edu.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Levis
keywords: Electrospray deposition; Femtosecond laser vaporization; Quantitative analysis