title: Associations of biochemical changes and maternal traits with mutation 1843 (C>T) in theRYR1gene as a common cause for porcine stress syndrome.
abstract: Stress syndrome is usually caused by a mutation in theryanodine receptorgene(ryr1) and it is widely studied in humans and swine populations. The protein product of this gene plays a crucial role in the regulation of calcium transport in muscle cells. A G>T mutation in the humanryr1gene, which results in the replacement of a conserved arginine at position 614 where a leucine occurs at the same position as the previously identified Arg→Cys mutation reported in all cases of porcine stress syndrome (PSS). Porcine stress syndrome affects biochemical pathways in stress-susceptible individuals during a stress episode and some biochemical parameters that were used as markers for diagnostic purposes. Also, PSS has remarkable influence on the maternal characteristics of sows. This study dealt with different genotypes for PSS and its association with possible biochemical changes and maternal traits of sows. Seventy-three reproductive sows genotyped for PSS by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were included in this survey. Sixty of them were stress-free (NN), 11 were heterozygous carriers (Nn) and two animals were homozygous (nn) for the 1843 (C>T) mutation. Significant differences in non stress induced animals with different PSS genotypes were found in the values of creatine phoshokinase (CPK), lactate dehydrogenase (LDH), alkaline phosphatase (AP) and aspartate aminotransferase (AST). Regarding the maternal traits, our study showed that stress susceptible animals (nn) have an increased number of stillborn piglets and a reduced number of newborn piglets compared with heterozygous and normal animals.
authors.affiliation: Department of Biochemistry and Genetic Engineering, Faculty of Agriculture and Food Sciences, Skopje, Republic of Macedonia.
authors.forename: Z T
authors.initials: ZT
authors.lastname: Popovski
authors.affiliation: Department of Biochemistry and Genetic Engineering, Faculty of Agriculture and Food Sciences, Skopje, Republic of Macedonia.
authors.forename: B
authors.initials: B
authors.lastname: Tanaskovska
authors.affiliation: Food Department, Faculty of Agriculture and Food Sciences, Skopje, Republic of Macedonia.
authors.forename: E
authors.initials: E
authors.lastname: Miskoska-Milevska
authors.affiliation: Livestock Department, Faculty of Agriculture and Food Sciences, Skopje, Republic of Macedonia.
authors.forename: S
authors.initials: S
authors.lastname: Andonov
authors.affiliation: Faculty of Medicine, Institute of Clinical Biochemistry, Skopje, Republic of Macedonia.
authors.forename: S
authors.initials: S
authors.lastname: Domazetovska
keywords: Biochemistry; Genotypes; Maternity; Porcine stress syndrome (PSS)