title: Accumulation of multiacetylated forms of histones by trichostatin A and its developmental consequences in early starfish embryos.
abstract: External application of 10 rig/ml (R)-trichostatin A (TSA), a potent and specific inhibitor of mammalian histone deacetylase, to the embryo of the starfish Asterina pectinifera inhibited development during the early gastrula stage before formation of mesenchyme cells. The TSA-sensitive period was limited to the mid-blastula stage before hatching. The pulse-chase experiment clearly demonstrated that TSA induced an accumulation of acetylated histone species in blastulae through inhibition of historic deacetylation. Similar blockage of development at the early gastrula stage was observed with n-butyrate, which has been known as a weak inhibitor of historic deacetylase. These results suggest an intimate role for historic acetylation-deacetylation equilibria in starfish development.
authors.affiliation: Department of Applied Biochemistry, Hiroshima University, 4-4, Kagamiyama 1 Chome, 724, Higashi-Hiroshima, Hiroshima, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikegami
authors.affiliation: Department of Applied Biochemistry, Hiroshima University, 4-4, Kagamiyama 1 Chome, 724, Higashi-Hiroshima, Hiroshima, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ooe
authors.affiliation: Department of Applied Biochemistry, Hiroshima University, 4-4, Kagamiyama 1 Chome, 724, Higashi-Hiroshima, Hiroshima, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Department of Applied Biochemistry, Hiroshima University, 4-4, Kagamiyama 1 Chome, 724, Higashi-Hiroshima, Hiroshima, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kasahara
authors.affiliation: Department of Applied Biochemistry, Hiroshima University, 4-4, Kagamiyama 1 Chome, 724, Higashi-Hiroshima, Hiroshima, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Tsuruta
authors.affiliation: Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, 113, Bunkyo-ku, Tokyo, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Kijima
authors.affiliation: Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, 113, Bunkyo-ku, Tokyo, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, 113, Bunkyo-ku, Tokyo, Japan.
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Beppu
keywords: Echinodermata; Embryos; Histone acetylation; Trichostatin A; n-Butyric acid