title: A quantitative method for separation of livingHydra cells.
abstract: We describe a rapid method for the isolation of large numbers of livingHydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNA-clones.
authors.affiliation: Zoologisches Institut der Universität München, Luisenstraße 14, W-8000, München 2, Germany.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Greber
authors.affiliation: Zoologisches Institut der Universität München, Luisenstraße 14, W-8000, München 2, Germany.
authors.forename: Charles N
authors.initials: CN
authors.lastname: David
authors.affiliation: Zoologisches Institut der Universität München, Luisenstraße 14, W-8000, München 2, Germany.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Holstein
keywords: Cell separation; Cell type specific gene expression; Counterflow centrifugation elutriation; Hydra