title: Induction of mitochondrial-dependent apoptosis in T24 cells by a selenium (Se)-containing polysaccharide from Ginkgo biloba L. leaves.
abstract: In the present study, a selenium (Se)-containing polysaccharide (Se-GBLP) was isolated and purified from the leaves of Ginkgo biloba L. Se-GBLP was further evaluated for its antitumor activity against human bladder cancer T24 cells together with the possible mechanism of action. Our results showed that treatment of T24 cells with Se-GBLP (50, 100 and 200μg/ml) for 48h significantly inhibited cell viability and induced apoptosis in a dose- dependent manner. This Se-GBLP-induced apoptosis is associated with an increased protein expression of pro-apoptotic Bax, decreased expression of anti-apoptotic Bcl-2, loss of mitochondrial membrane potential, and cleavage of caspase-9, caspase-3 and PARP, suggesting that Se-GBLP-induced apoptosis occurs through the mitochondria-dependent pathway. Se-GBLP therefore merits further investigation as a promising preventive and/or therapeutic agent against human bladder cancer.
authors.affiliation: Department of urological surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of urological surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of urological surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Cai
authors.affiliation: Department of urological surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China. Electronic address: guangqikong@126.com.
authors.forename: Guangqi
authors.initials: G
authors.lastname: Kong
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D059748:Proteolysis / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012643:Selenium / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D053453:Caspase 9; D012643:Selenium
keywords: Ginkgo biloba L.; Human bladder cancer; Selenium-polysaccharides