title: Immunologic regulatory effects of human umbilical cord blood-derived mesenchymal stem cells in a murine ovalbumin asthma model.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) have multiple immunomodulatory properties and hold therapeutic potential for inflammatory diseases. However, the therapeutic and immunologic effects of human umbilical cord blood-derived MSCs (huMSCs) remain largely unexamined for asthma.
  
  
  OBJECTIVE
  This study was to investigate the immunomodulatory properties of huMSCs in an ovalbumin (OVA)-induced murine asthma model.
  
  
  METHODS
  Mice were injected intraperitoneally with OVA and an aluminium hydroxide adjuvant. huMSCs were administered via the tail vein (5×105cells/100 uL) to female BALB/c mice prior to the initial OVA challenge. The effects of huMSCs were assessed by investigating airway hyperresponsiveness, histological changes, inflammatory cell numbers, serum allergen-specific antibodies, cytokine production in spleen, lung tissue, and bronchoalveolar lavage (BAL) fluid as well as expansion of regulatory T cells.
  
  
  RESULTS
  Administration of huMSCs significantly reduced methacholine bronchial hyperresponsiveness and eosinophil counts in BAL cells. Similarly, there was a significant decrease in serum OVA-specific IgE and IgG1 levels along with Th2 cytokine production (IL-4, IL-5, and IL-13) in the lung and spleen tissues, whereas increased percentage of regulatory T cells was observed after treatment with huMSCs.
  
  
  CONCLUSIONS
  Our results suggest that huMSC treatment reduces OVA-induced allergic inflammation, which could be mediated by regulatory T cells.
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: S-Y
authors.identifier: http://orcid.org/0000-0001-5505-3028
authors.initials: SY
authors.lastname: Kang
authors.affiliation: Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: D-E
authors.initials: DE
authors.lastname: Park
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: W-J
authors.identifier: http://orcid.org/0000-0002-4630-9922
authors.initials: WJ
authors.lastname: Song
authors.affiliation: Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: B-R
authors.initials: BR
authors.lastname: Bae
authors.affiliation: Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: J-W
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: K-H
authors.initials: KH
authors.lastname: Sohn
authors.affiliation: Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: H-S
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: H-R
authors.initials: HR
authors.lastname: Kang
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: H-W
authors.initials: HW
authors.lastname: Park
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.|Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Y-S
authors.initials: YS
authors.lastname: Chang
authors.affiliation: Biomedical Research Institute, MEDIPOST, Co., Ltd., Seoul, Korea.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Biomedical Research Institute, MEDIPOST, Co., Ltd., Seoul, Korea.
authors.forename: W-I
authors.initials: WI
authors.lastname: Oh
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: K-U
authors.initials: KU
authors.lastname: Min
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: S-H
authors.identifier: http://orcid.org/0000-0002-7644-6469
authors.initials: SH
authors.lastname: Cho
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D056747:Immunomodulation; D018836:Inflammation Mediators / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D016210:Methacholine Chloride / Q000378:metabolism; D051379:Mice; D010047:Ovalbumin / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
chemical_list: D000485:Allergens; D016207:Cytokines; D018836:Inflammation Mediators; D016210:Methacholine Chloride; D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: animal models; asthma; clinical immunology; mesenchymal stem cell; mice; umbilical cord