title: Stilbenes from Vitis vinifera L. Waste: A Sustainable Tool for Controlling Plasmopara Viticola.
abstract: Stilbene-enriched extracts from Vitis vinifera waste (cane, wood, and root) were characterized by UHPLC-MS. Eleven stilbenes were identified and quantified as follows: ampelopsin A, (E)-piceatannol, pallidol, (E)-resveratrol, hopeaphenol, isohopeaphenol, (E)-ε-viniferin, (E)-miyabenol C, (E)-ω-viniferin, r2-viniferin, and r-viniferin. The fungicide concentration inhibiting 50% of growth of Plasmopara viticola sporulation (IC50) was determined for the extracts and also for the main compounds isolated. r-Viniferin followed by hopeaphenol and r2-viniferin showed low IC50and thus high efficacy against Plasmopara viticola. Regarding stilbene extracts, wood extract followed by root extract showed the highest antifungal activities. These data suggest that stilbene complex mixtures from Vitis vinifera waste could be used as a cheap source of bioactive stilbenes for the development of natural fungicides.
authors.affiliation: Université de Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (Gesvab), Institut des Sciences de la Vigne et du Vin , CS 50008-210, Chemin de Leysotte, 33882 Villenave d'Ornon, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Gabaston
authors.affiliation: Instituto de Investigación y Formación Agraria y Pesquera (IFAPA) , Rancho de la Merced, Junta de Andalucía, Ctra. Trebujena, Km 3.2, P.O. Box 589, Jerez de la Frontera, 11471 Cádiz, Spain.
authors.forename: Emma
authors.initials: E
authors.lastname: Cantos-Villar
authors.affiliation: Université de Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (Gesvab), Institut des Sciences de la Vigne et du Vin , CS 50008-210, Chemin de Leysotte, 33882 Villenave d'Ornon, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Biais
authors.affiliation: Université de Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (Gesvab), Institut des Sciences de la Vigne et du Vin , CS 50008-210, Chemin de Leysotte, 33882 Villenave d'Ornon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Waffo-Teguo
authors.affiliation: Université de Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (Gesvab), Institut des Sciences de la Vigne et du Vin , CS 50008-210, Chemin de Leysotte, 33882 Villenave d'Ornon, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Renouf
authors.affiliation: UMR Santé Végétale, 1065, ISVV, IFR 103, INRA , Rue Edouard Bourleaud, CS 20032, 33882 Villenave d' Ornon, France.
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Corio-Costet
authors.affiliation: Université de Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (Gesvab), Institut des Sciences de la Vigne et du Vin , CS 50008-210, Chemin de Leysotte, 33882 Villenave d'Ornon, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Richard
authors.affiliation: Université de Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (Gesvab), Institut des Sciences de la Vigne et du Vin , CS 50008-210, Chemin de Leysotte, 33882 Villenave d'Ornon, France.
authors.forename: Jean-Michel
authors.identifier: http://orcid.org/0000-0002-9626-2149
authors.initials: JM
authors.lastname: Mérillon
mesh_terms: D005659:Fungicides, Industrial / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D009868:Oomycetes / Q000187:drug effects* / Q000254:growth & development; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000382:microbiology; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
chemical_list: D005659:Fungicides, Industrial; D010936:Plant Extracts; D013267:Stilbenes; D014866:Waste Products
keywords: UHPLC-MS; antifungal activity; cane; grapevine waste; mildew; root; wood