title: Mapping New Residents of the Mitochondrial Nucleoid.
abstract: In this issue of Cell Chemical Biology, Han et al. (2017) used a powerful combination of quantitative ratiometric mass spectrometry with APEX peroxidase-catalyzed proximity biotinylation to selectively highlight proteins associated with mitochondrial DNA above the background of contaminants and matrix proteins. In addition to identifying novel nucleoid factors, this study extends the APEX strategy to the proteomic mapping of non-membrane-bound multiprotein complexes.
authors.affiliation: Department of Cellular and Molecular Medicine and Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada. Electronic address: ltrinkle@uottawa.ca.
authors.forename: Laura
authors.initials: L
authors.lastname: Trinkle-Mulcahy
mesh_terms: D019921:Biotinylation; D004272:DNA, Mitochondrial; D008928:Mitochondria / Q000235:genetics; D011506:Proteins; D040901:Proteomics
chemical_list: D004272:DNA, Mitochondrial; D011506:Proteins