title: Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains.
abstract: Antibody-based molecular switches that are able to recognize a range of exogenous antigens can be highly useful as a versatile biosensor. However, regulating the catalytic activity of enzymes by antibodies is still hard to achieve. Here, we describe a design method of unique antibody variable region Fv introduced with two circular permutations, called Clampbody. By tethering the Clampbody to a circularly permuted TEM-1 β-lactamase (BLA), we successfully constructed a genetically encoded molecular switch Cbody-cpBLA that shows antigen-dependent catalytic activity.
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Iwai
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Kojima-Misaizu
authors.affiliation: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259-R1-18 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan.|College of Chemistry and Chemical Engineering, Linyi University, Shandong, 27600, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Dong
authors.affiliation: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259-R1-18 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan. ueda@res.titech.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
mesh_terms: D000906:Antibodies / Q000235:genetics*; D002384:Catalysis; D007128:Immunoglobulin Fragments / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000906:Antibodies; D007128:Immunoglobulin Fragments; D011993:Recombinant Fusion Proteins; C055966:immunoglobulin Fv; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1
keywords: Allosteric regulation; Antibody variable region; Circular permutation; Immunosensor; TEM-1 β-lactamase