title: Cell-specific endopolyploidy in developing Artemia.
abstract: Cells in developing Artemia franciscana SFB demonstrated tissue-specific differences in DNA content, as determined by fluorescence intensity of bisbenzimide-stained nuclei and by nuclear area. The general epidermis comprised proliferating diploid (2C) cells. The setal cells had 4C-8C DNA content and did not divide during the first two instars. Salt gland cells were polyploid (>8C) and also did not undergo mitosis. Neural cells in the brain were diploid and were replicating. Cells in the thorax region of the gut had a 4C-8C DNA content and were proliferating. The muscle cells in the cephalic appendages contained 2C non-replicating nuclei. Only diploid epidermal cells were involved in segment morphogenesis. There was no difference in number of chromosomes (n=42) in the epidermal cells and the gut cells, indicating that the tissue-specific endopolyploidy was due to endoreduplication.
authors.affiliation: Department of Biology (JAF), University of South Alabama, 36688, Mobile, AL, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Freeman
authors.affiliation: Department of Anatomy and Cell Biology (RBC), University of South Alabama, 36688, Mobile, AL, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Chronister
keywords: Artemia; Differentiation; Endopolyploidy