title: The arrest of embryogenesis at gastrula stage in the diapausing silkwormBombyx mori is related to the synthesis of protein P61.
abstract: Two-dimensional protein gels are used to assess systematically changes in protein synthesis in diapausing and non-diapausing early embryos ofBombyx mori throughout natural breakage of diapause by chilling and after artificial prevention of diapause by HCl+ heat-shock treatment. A set of proteins, the heat-shock protein (hsp) 70 family previously described, was synthesized in diapausing and non-diapausing development at the early germ-anlage stage; by contrast, protein 61 (P61; 61 kDa) was synthesized only in the diapausing gastrula stage. The synthesis of P61 decreased during days at 5°C. Thereafter, we observed the synthesis of the hsp 70 family at 5°C. After the artificial prevention of diapause, P61 synthesis was not induced at the gastrula stage, whereas the hsp 70 family was enhanced in the 6 h following the preventive treatment. P61 was only synthesized after treatments were ineffective in preventing diapause and in inducing hsp 70. Thus, P61 can be described as a "diapause arrest-associated protein."
authors.affiliation: Centre de Génétique Moleculaire et Cellulaire UMR 106 CNRS, Université Claude Bernard Lyon I, ICBMC, 43 Bd. du 11 Novembre 1918, F-69622, Villeubranne, France.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Coulon-Bublex
authors.affiliation: Centre de Génétique Moleculaire et Cellulaire UMR 106 CNRS, Université Claude Bernard Lyon I, ICBMC, 43 Bd. du 11 Novembre 1918, F-69622, Villeubranne, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Dorel
keywords: Arrest in diapause; Bombyx mori; Heat-shock; Insect embryo; Protein pattern