title: Differential lectin-mediated agglutinabilities of the embryonic and the first extraembryonic cell line of the early chick embryo.
abstract: The lectin-mediated agglutinability of cells dissociated from different areas of the gastrulating chick embryo was investigated. Differences in agglutinability were quantified by using a Coulter counter. Cells from the area pellucida (AP) and those from the endoderm of the area opaca (AOEn) are agglutinated by Concanavalin A (Con A), wheat germ agglutinin (WGA) andRicinus communis agglutinin (RCA). In cells from both areas the greatest agglutination response is obtained with RCA. Trypsinization of AOEn cells enhances their agglutinability with Con A, WGA and RCA. The lectin-induced agglutinability of cells from the area pellucida is similar in EDTA-dissociated and trypsinized cells.Cells from the AP are significantly more agglutinable with Con A than those of the AOEn regardless whether the former are obtained by trypsinization or dissociation with EDTA. The higher agglutinability of cells of the area pellucida with Con A, as well as the differential enhancement by trypsin of the agglutinability of AOEn cells with Con A, WGA, and RCA may reflect a difference in the cell surface glycoreceptors between the cells of the are pellucida (predominantly embryonic) and the first extraembryonic (AOEn) cell line. These cells have been shown to sort out from each other at the earliest stages of development.
authors.affiliation: Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada.
authors.forename: John Robert
authors.initials: JR
authors.lastname: Phillips
authors.affiliation: Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Zalik
keywords: Agglutination; Blastoderm cell surface; Lectins