title: Transcription FactorOsDOF18Controls Ammonium Uptake by Inducing Ammonium Transporters in Rice Roots.
abstract: Nitrogen is one of the most important mineral elements for plant growth. We studied the functional roles ofOryza sativa DNA BINDING WITH ONE FINGER 18(OsDOF18) in controlling ammonium uptake. The growth of null mutants ofOsDOF18was retarded in a medium containing ammonium as the sole nitrogen source. In contrast, those mutants grew normally in a medium with nitrate as the sole nitrogen source. The gene expression was induced by ammonium but not by nitrate. Uptake of ammonium was lower inosdof18mutants than in the wild type, while that of nitrate was not affected by the mutation. This indicated that OsDOF18 is involved in regulating ammonium transport. Among the 10 ammonium transporter genes examined here, expression ofOsAMT1;1,OsAMT1;3,OsAMT2;1, andOsAMT4;1was reduced inosdof18mutants, demonstrating that the ammonium transporter genes function downstream ofOsDOF18. Genes for nitrogen assimilation were also affected in the mutants. These results provide evidence thatOsDOF18mediates ammonium transport and nitrogen distribution, which then affects nitrogen use efficiency.
authors.affiliation: Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea.
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Yang
authors.affiliation: Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea.
authors.forename: Jinhuan
authors.initials: J
authors.lastname: Wei
authors.affiliation: Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea.
authors.forename: Hyeryung
authors.initials: H
authors.lastname: Yoon
authors.affiliation: Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea.
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D064751:Ammonium Compounds; D002352:Carrier Proteins; D009566:Nitrates; D010940:Plant Proteins; D014157:Transcription Factors
keywords: ammonium; ammonium transporters; nitrate; rice; transcription factor DOF