title: The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs.
abstract: Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs' proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions. In addition, high-throughput microarray analysis showed that gene expressions of RCCS-3D system displayed significant differences in cell proliferation and differentiation compared with static-2D conditions. Thus, RCCS-3D system could provide an effective means for BMSCs cell proliferation in vitro and meanwhile maintain differentiation potential in tissue engineering.
authors.affiliation: Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.|Department of Spine Surgery, Sichuan Provincial Orthopedic Hospital, Chengdu, 610041, People's Republic of China.
authors.forename: Yilong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.|Department of Spine Surgery, Dali Bai Autonomous Prefecture People's Hospital, Dali, 671000, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.|Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.|Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Han
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, College of Life Sciences, Shandong Agricultural University, Taian, 271018, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dai
authors.affiliation: Department of Oncology, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China. whwl2003@tom.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China. jwdai@genetics.ac.cn.|Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, People's Republic of China. jwdai@genetics.ac.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China. whwl@hotmail.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds