title: Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA).
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in hospitals and the community. As the rapid spread and wide distribution of antimicrobial resistance (such as MRSA), treatment for infectious diseases caused by microorganisms has become a vital threat. Thus, early identification and genotyping are essential for further therapeutic treatment and the control of rapid expansion of MRSA. In combination with applications and data feedbacks, this review focused on the currently available molecular-based assays on their utility and performance for rapid typing of MRSA, especially on effective molecular-based methods. Besides, a common mobile element SCCmec and prevalence of HA-MRSA, LA-MRSA and CA-MRSA were introduced in this review in order to provide a more complete profile of MRSA.
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Miao
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Lequn
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, PR China.
authors.forename: Dingqiang
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; State Key Laboratory of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co. Ltd., Qingdao 266061, PR China. Electronic address: dengyangscut@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China; Department of Microbial Pathogenesis, University of Maryland, Baltimore 21201, USA. Electronic address: zhenbo.xu@hotmail.com.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Xu
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D003030:Coagulase / Q000235:genetics; D003428:Cross Infection / Q000175:diagnosis / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D058885:Multilocus Sequence Typing / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D046915:Penicillin-Binding Proteins / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D015534:Trans-Activators / Q000235:genetics
chemical_list: C095698:Agr protein, Staphylococcus aureus; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003030:Coagulase; D046915:Penicillin-Binding Proteins; D015534:Trans-Activators; C487789:mecA protein, Staphylococcus aureus; C119204:streptococcal protective antigen
keywords: MRSA; Molecular methods; SCCmec; Typing