title: Generation of Lactobacillus plantarum strains with improved potential to target gastrointestinal disorders related to sugar malabsorption.
abstract: Malabsorption of dietary sugars is a common cause of gastrointestinal discomfort, affecting up to one in three people with debilitating symptoms, such as abdominal pain, osmotic diarrhoea, bloating and flatulence. Besides dietary interventions, it has been suggested that ingestion of lactobacilli may alleviate these symptoms. The objectives of this study were to generate strains with improved potential to ameliorate sugar malabsorption related gastrointestinal disorders. Initial selection was made from 183 natural isolates of lactic acid bacteria, on the basis of broad sugar fermentation ability, absence of gas production, gastrointestinal survival and susceptibility to important medical antimicrobials. Two strains of L. plantarum (KR6 and M5) exhibited favourable characteristics for all criteria, and were further optimised through random mutagenesis and selection approaches. Ultraviolet light (UV) exposure resulted in mutants characterized by better survival (for 1.9 log and 1.4 log) in gastrointestinal conditions. Subsequent exposure to ethyl methanesulfonate (EMS) provided mutants with greater tolerance to glucose induced catabolic repression. UV and UV-EMS mutants of L. plantarum M5 showed improved adhesion ability. As a result of this optimisation, L. plantarum MP2026 and L. plantarum MP2420 have been identified as promising candidates for probiotics, intended for alleviation of gastrointestinal discomfort originating from sugar malabsorption.
authors.affiliation: Acies Bio, d.o.o., Tehnološki park 21, 1000 Ljubljana, Slovenia.
authors.forename: Helena
authors.initials: H
authors.lastname: Šeme
authors.affiliation: University of Ljubljana, Biotechnical Faculty, Institute of Dairy Science and Probiotics, Groblje 3, 1230 Domžale, Slovenia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Bogovič Matijašić
authors.affiliation: Medis, d.o.o., Brnčičeva 1, 1231 Ljubljana, -Črnuče, Slovenia.
authors.forename: Karmen
authors.initials: K
authors.lastname: Švigelj
authors.affiliation: University of Maribor, Faculty of Agriculture and Life Sciences, Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Pivola 10, 2311 Hoče, Slovenia.
authors.forename: Tomaž
authors.initials: T
authors.lastname: Langerholc
authors.affiliation: Acies Bio, d.o.o., Tehnološki park 21, 1000 Ljubljana, Slovenia.
authors.forename: Štefan
authors.initials: Š
authors.lastname: Fujs
authors.affiliation: Acies Bio, d.o.o., Tehnološki park 21, 1000 Ljubljana, Slovenia.
authors.forename: Jaka
authors.initials: J
authors.lastname: Horvat
authors.affiliation: University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Emil
authors.initials: E
authors.lastname: Zlatić
authors.affiliation: Acies Bio, d.o.o., Tehnološki park 21, 1000 Ljubljana, Slovenia.
authors.forename: Krešimir
authors.initials: K
authors.lastname: Gjuračić
authors.affiliation: Acies Bio, d.o.o., Tehnološki park 21, 1000 Ljubljana, Slovenia; University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Petković
authors.affiliation: Medis, d.o.o., Brnčičeva 1, 1231 Ljubljana, -Črnuče, Slovenia.
authors.forename: Mateja
authors.initials: M
authors.lastname: Štempelj
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Pierottijeva 6, 10000 Zagreb, Croatia.
authors.forename: Blaženka
authors.initials: B
authors.lastname: Kos
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Pierottijeva 6, 10000 Zagreb, Croatia.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Šušković
authors.affiliation: Acies Bio, d.o.o., Tehnološki park 21, 1000 Ljubljana, Slovenia. Electronic address: gregor.kosec@aciesbio.com.
authors.forename: Gregor
authors.initials: G
authors.lastname: Kosec
mesh_terms: D015746:Abdominal Pain / Q000209:etiology / Q000382:microbiology / Q000517:prevention & control; D001422:Bacterial Adhesion; D050260:Carbohydrate Metabolism; D002460:Cell Line; D000073417:Dietary Sugars / Q000378:metabolism*; D005285:Fermentation; D005414:Flatulence; D005767:Gastrointestinal Diseases / Q000209:etiology / Q000382:microbiology* / Q000517:prevention & control; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D007422:Intestines / Q000166:cytology / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D008286:Malabsorption Syndromes / Q000150:complications*; D016296:Mutagenesis; D009154:Mutation; D019936:Probiotics; D013045:Species Specificity
chemical_list: D000073417:Dietary Sugars; D005947:Glucose
keywords: Adhesion to H4-1 intestinal cells; Catabolite repression; Lactobacillus plantarum; Probiotic; Random mutagenesis; Sugar malabsorption