title: Gas Chromatography-Based Ethylene Measurement of Arabidopsis Seedlings.
abstract: Plants tightly regulate the biosynthesis of ethylene to control growth and development and respond to a wide range of biotic and abiotic stresses. To understand the molecular mechanism by which plants regulate ethylene biosynthesis as well as to identify stimuli triggering the alteration of ethylene production in plants, it is essential to have a reliable tool with which one can directly measure in vivo ethylene concentration. Gas chromatography is a routine detection technique for separation and analysis of volatile compounds with relatively high sensitivity. Gas chromatography has been widely used to measure the ethylene produced by plants, and has in turn become a valuable tool for ethylene research. Here, we describe a protocol for measuring the ethylene produced by dark-grown Arabidopsis seedlings using a gas chromatograph.
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, 915 West State St., West Lafayette, IN, 47907, USA. yoong@purdue.edu.
authors.forename: Gyeong Mee
authors.initials: GM
authors.lastname: Yoon
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, 915 West State St., West Lafayette, IN, 47907, USA.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002849:Chromatography, Gas / Q000379:methods; D005030:Ethylenes / Q000032:analysis / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000032:analysis / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene
keywords: ACC synthase; Arabidopsis; Dark-grown seedlings; Ethylene; Gas chromatography