title: Long-term effect of sildenafil on echocardiographic parameters in dogs with asymptomatic myxomatous mitral valve degeneration.
abstract: Sildenafil is a selective phosphodiesterase-5 inhibitor that has been demonstrated to delay ventricular remodeling in humans and experimental animals. The aim of this prospective study was to assess the chronic effects of sildenafil administration on echocardiographic indices and N-terminal pro-B-type natriuretic peptide (NT-proBNP) in dogs with naturally occurring, asymptomatic myxomatous mitral valve degeneration. Thirty client-owned dogs with ACVIM class B1 or B2 were enrolled. Dogs were randomly assigned to treatment (sildenafil 1-3 mg/kg, PO, BID for 180 days) or control groups. A total of 12 dogs completed the 180 days trial in the sildenafil group, whereas 10 dogs remained in control group. When comparing the difference from baseline values obtained over time between groups, the stroke volume (SV) at day 30 was significantly higher in the sildenafil group (P=0.038). The LA/Ao and the MR jet area were significantly lower beginning at day 30 (only MR jet area; P=0.006), day 90 (P=0.006 and P=0.027, respectively) and day 180 (P=0.029 and P=0.032, respectively). The 2D-LA was significantly lower at day 90 when compared with control group (P=0.028). The differences of NTproBNP from baseline were significantly lower when compared with control group at the same timepoint (D90, P=0.017 and D180, P=0.013). In conclusion, this study suggested that long-term treatment with sildenafil prevented aggravation of disease progression as suggested by several echocardiographic indices (i.e. SV, LA/Ao, MR jet area, 2D-LA) and reduced NTproBNP level at the indicated timepoints in dogs with asymptomatic mitral valve degeneration.
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.|Research clusters: Research study and testing of drug's effect related to cardiovascular system in Laboratory Animal, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Anusak
authors.initials: A
authors.lastname: Kijtawornrat
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Siripen
authors.initials: S
authors.lastname: Komolvanich
authors.affiliation: Department of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Nakkawee
authors.initials: N
authors.lastname: Saengklub
authors.affiliation: Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
authors.forename: Prapawadee
authors.initials: P
authors.lastname: Pirintr
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pakit
authors.initials: P
authors.lastname: Boonpala
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Chollada
authors.initials: C
authors.lastname: Buranakarl
mesh_terms: D000818:Animals; D018450:Disease Progression; D004283:Dog Diseases / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D004285:Dogs; D004452:Echocardiography / Q000662:veterinary; D004562:Electrocardiography / Q000662:veterinary; D005260:Female; D008297:Male; D008944:Mitral Valve Insufficiency / Q000097:blood / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000662:veterinary*; D020097:Natriuretic Peptide, Brain / Q000097:blood; D058986:Phosphodiesterase 5 Inhibitors / Q000627:therapeutic use*; D011446:Prospective Studies; D011859:Radiography / Q000662:veterinary; D000068677:Sildenafil Citrate / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: D058986:Phosphodiesterase 5 Inhibitors; D020097:Natriuretic Peptide, Brain; D000068677:Sildenafil Citrate
keywords: asymptomatic; dog; echocardiography; mitral valve degeneration; sildenafil