title: A naturally occurring conditional albino mutant in rice caused by defects in the plastid-localized OsABCI8 transporter.
abstract: A wide range of molecules are transported across membranes by the ATP binding cassette (ABC) transporters. Plants possess a collection of ABC proteins bearing similarities to the components of prokaryotic multi subunit ABC transporters, designed as ABC group I. However the functions of most of them are not well understood. Here, we characterized a naturally occurring rice mutant that exhibited albino phenotype under continuous rainy days in the field, but gradually recovered to normal green after the rainy season. Molecular and genetic analyses revealed that the phenotypes were caused by a mutation in the OsABCI8 that encoded a member of the ABCI family. Subcellular localization demonstrated that OsABCI8 is a chloroplast ABC transporter. Expression of OsABCI8 is significantly enhanced in rainy days compared to sunny days. Besides defects in chloroplast development and chlorophyll biosynthesis, the mutant phenotype is accompanied by a higher accumulation of iron, suggesting that OsABCI8 is involved in iron transportation and/or homeostasis in rice. Our results demonstrate that OsABCI8 represents a conserved ABCI protein involved in transition metals transportation and/or homeostasis and suggest an important role of the plastid-localized OsABCI8 for chloroplast development.
authors.affiliation: Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Xiuyu
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Tang
authors.affiliation: Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Herong
authors.initials: H
authors.lastname: Guo
authors.affiliation: Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Shanwen
authors.initials: S
authors.lastname: Ke
authors.affiliation: Anhui Provincial Key Laboratory of Rice Genetics and Breeding, Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Teng
authors.affiliation: Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, 28081, USA.|Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, 27695, USA.
authors.forename: Yu-Hung
authors.initials: YH
authors.lastname: Hung
authors.affiliation: Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Zhenjiang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Xie
authors.affiliation: Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, 28081, USA. thsieh3@ncsu.edu.|Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, 27695, USA. thsieh3@ncsu.edu.
authors.forename: Tzung-Fu
authors.initials: TF
authors.lastname: Hsieh
authors.affiliation: Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. aacrav@163.com.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D007501:Iron / Q000378:metabolism; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018087:Plastids / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; D007501:Iron
keywords: ABC transporter; Chloroplast development; Iron transport; Rice