title: Selenium and a newly synthesized Thiocyanoacetamide reduce Doxorubicin gonadotoxicity in male rat.
abstract: Despite its deleterious effect on healthy cells and highly regenerating cells such as spermatozoa, Doxorubicin (DOX) is still one of the most used anticancer drugs in the last decades. The present work aimed to investigate the ability of the selenium (Se) and the thiocyanoacetamide (T) to reduce DOX toxicity in gonad. Adult male rats were treated with DOX intravenously (i.v.) at 3.7mg/kg/week associated with Se intragastrically (i.g.) at 0.2mg/kg/day or with T at 10mg/kg/day i.g. After 47days of treatment, sperm quality, biochemical parameters, blood cell count and histological changes in liver, testis and epididymis were assessed. The results showed a poor sperm quality, a perturbation of ionic stability and a significant alteration of lipid metabolism and hematological parameters after the sub-chronic administration of DOX. In testis, DOX exerted serious epithelium damage and numerous seminiferous tubules did not present a normal spermatogenesis. In epididymis, epithelium was altered and mastocytes infiltrated the interstitium. DOX did not exert any significant change in liver except dilatations of sinusoid capillaries. DOX association with Se or T reduced its toxicity on some hematological and biochemical parameters. Both combined treatment improved sperm quality and partially restored spermatogenesis as well as testis and epididymis' normal aspect. These findings brought new sights regarding the effect of Se and a new derivative of T in a combined treatment with DOX on germ cells, gonad and liver. The support of these relevant outcomes with further in vitro studies is necessary to highlight the accurate process involved in Se or T protection against DOX induced damages.
authors.affiliation: Histology, Embryology and Cell Biology Laboratory, Medicine School, University of Tunis El Manar, Jabbari Jebel Lakhdar Street 15, 1007 Tunis, Tunisia. Electronic address: boussadamarwa@gmail.com.
authors.forename: Marwa
authors.initials: M
authors.lastname: Boussada
authors.affiliation: Experimental Medicine Unit, Medicine School, University of Tunis El Manar, Jabbari Jebel Lakhdar Street 15, 1007 Tunis, Tunisia. Electronic address: ridhabenali9@gmail.com.
authors.forename: Ridha Ben
authors.initials: RB
authors.lastname: Ali
authors.affiliation: Central Preparation Unit of Cytotoxic, Salah Azaiz Institute, Bab Saâdoun Square, 1006 Tunis, Tunisia. Electronic address: azza-bensaid@yahoo.fr.
authors.forename: Azaa Ben
authors.initials: AB
authors.lastname: Said
authors.affiliation: Laboratory of Organic Synthesis and Heterocyclic Chemistry Department, School of Sciences of Tunis, University of Tunis El Manar, 2092 Tunis, Tunisia.
authors.forename: Khouloud
authors.initials: K
authors.lastname: Bokri
authors.affiliation: Laboratory of Organic Synthesis and Heterocyclic Chemistry Department, School of Sciences of Tunis, University of Tunis El Manar, 2092 Tunis, Tunisia. Electronic address: azaiezbenakacha@yahoo.fr.
authors.forename: Azaiez Ben
authors.initials: AB
authors.lastname: Akacha
authors.affiliation: Experimental Medicine Unit, Medicine School, University of Tunis El Manar, Jabbari Jebel Lakhdar Street 15, 1007 Tunis, Tunisia.
authors.forename: Chedli
authors.initials: C
authors.lastname: Dziri
authors.affiliation: Experimental Medicine Unit, Medicine School, University of Tunis El Manar, Jabbari Jebel Lakhdar Street 15, 1007 Tunis, Tunisia. Electronic address: elmay_michele@yahoo.fr.
authors.forename: Michèle Véronique
authors.initials: MV
authors.lastname: El May
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D004317:Doxorubicin / Q000633:toxicity*; D008297:Male; D009570:Nitriles / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects*; D059005:Topoisomerase II Inhibitors / Q000633:toxicity
chemical_list: D000975:Antioxidants; D009570:Nitriles; D059005:Topoisomerase II Inhibitors; D004317:Doxorubicin; D012643:Selenium
keywords: Doxorubicin; Selenium; Sperm; Testes; Thiocyanoacetamide; in vivo