title: Effect of Meso vs Macro Size of Hierarchical Porous Silica on the Adsorption and Activity of Immobilized β-Galactosidase.
abstract: β-Galactosidase (β-Gal) is one of the most important enzymes used in milk processing for improving their nutritional quality and digestibility. Herein, β-Gal has been entrapped into a meso-macroporous material (average pore size 9 and 200 nm, respectively) prepared by a sol-gel method from a silica precursor and a dispersion of solid lipid nanoparticles in a micelle phase. The physisorption of the enzyme depends on the concentration of the feed solution and on the pore size of the support. The enzyme is preferentially adsorbed either in mesopores or in macropores, depending on its initial concentration. Moreover, this selective adsorption, arising from the oligomeric complexation of the enzyme (monomer/dimer/tetramer), has an effect on the catalytic activity of the material. Indeed, the enzyme encapsulated in macropores is more active than the enzyme immobilized in mesopores. Designed materials containing β-Gal are of particular interest for food applications and potentially extended to bioconversion, bioremediation, or biosensing when coupling the designed support with other enzymes.
authors.affiliation: SRSMC UMR 7565 CNRS-Université de Lorraine, Bvd des Aiguillettes, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France.
authors.forename: Ileana-Alexandra
authors.initials: IA
authors.lastname: Pavel
authors.affiliation: Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá , E-28871 Alcala de Henares, Spain.
authors.forename: Sofia F
authors.initials: SF
authors.lastname: Prazeres
authors.affiliation: Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá , E-28871 Alcala de Henares, Spain.|University Institute of Research in Police Sciences (IUICP) , E-28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Montalvo
authors.affiliation: Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá , E-28871 Alcala de Henares, Spain.|University Institute of Research in Police Sciences (IUICP) , E-28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Garcı A Ruiz
authors.affiliation: Institut Jean Lamour UMR 7198 CNRS-Université de Lorraine, ENSTIB, 27 rue Philippe Séguin, CS 60036, 88026 Cedex Epinal, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Nicolas
authors.affiliation: Institut Jean Lamour UMR 7198 CNRS-Université de Lorraine, ENSTIB, 27 rue Philippe Séguin, CS 60036, 88026 Cedex Epinal, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Celzard
authors.affiliation: SRSMC UMR 7565 CNRS-Université de Lorraine, Bvd des Aiguillettes, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France.
authors.forename: François
authors.initials: F
authors.lastname: Dehez
authors.affiliation: LRGP UMR 7274 CNRS-Université de Lorraine, ENSAIA, 2, avenue de la forêt de Hayes, 54500 Vandoeuvre-lès-Nancy, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Canabady-Rochelle
authors.affiliation: SRSMC UMR 7565 CNRS-Université de Lorraine, Bvd des Aiguillettes, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France.
authors.forename: Nadia
authors.identifier: 0000-0002-7464-8067
authors.initials: N
authors.lastname: Canilho
authors.affiliation: SRSMC UMR 7565 CNRS-Université de Lorraine, Bvd des Aiguillettes, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France.
authors.forename: Andreea
authors.initials: A
authors.lastname: Pasc
mesh_terms: D000327:Adsorption; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D001616:beta-Galactosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D001616:beta-Galactosidase