title: A novel cetacean adenovirus in stranded harbour porpoises from the North Sea: detection and molecular characterization.
abstract: Harbour porpoises (Phocoena phocoena) are the most prevalent cetaceans in the North Sea. The fecal viral flora of 21 harbour porpoises stranded along the Dutch coastline was analyzed by a metagenomics approach. Sequences of a novel cetacean mastadenovirus, designated harbour porpoise adenovirus 1 (HpAdV-1), were detected. The sequence of a 23-kbp genomic region, spanning the conserved late region, was determined using primer walking. Phylogenetic analysis indicated that HpAdV-1 is most closely related to bottlenose dolphin adenovirus and clusters with Cetartiodactyla adenoviruses. The prevalence of HpAdV-1 was low (2.6%) based on targeted PCR-screening of the intestinal contents of 151 harbour porpoises stranded between 2010 and 2013.
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.158, 3508 TD, Utrecht, The Netherlands.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: van Beurden
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.158, 3508 TD, Utrecht, The Netherlands.
authors.forename: Lonneke L
authors.initials: LL
authors.lastname: IJsseldijk
authors.affiliation: Department of Viroscience, Erasmus Medical Center, P.O. Box 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: Marco W G
authors.initials: MWG
authors.lastname: van de Bildt
authors.affiliation: Department of Viroscience, Erasmus Medical Center, P.O. Box 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: Lineke
authors.initials: L
authors.lastname: Begeman
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: James F X
authors.initials: JFX
authors.lastname: Wellehan
authors.affiliation: College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610, USA.
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Waltzek
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.158, 3508 TD, Utrecht, The Netherlands.
authors.forename: Geert
authors.initials: G
authors.lastname: de Vrieze
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.158, 3508 TD, Utrecht, The Netherlands.
authors.forename: Andrea
authors.initials: A
authors.lastname: Gröne
authors.affiliation: Department of Viroscience, Erasmus Medical Center, P.O. Box 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: Thijs
authors.initials: T
authors.lastname: Kuiken
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.158, 3508 TD, Utrecht, The Netherlands. m.h.verheije@uu.nl.
authors.forename: M Hélène
authors.initials: MH
authors.lastname: Verheije
authors.affiliation: INRS-Institut Armand-Frappier, 531 Boul des Prairies, Laval, QC, H7V 1B7, Canada.
authors.forename: Judit J
authors.initials: JJ
authors.lastname: Penzes
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000662:veterinary*; D000595:Amino Acid Sequence; D000820:Animal Diseases / Q000821:virology*; D000818:Animals; D005243:Feces / Q000821:virology; D056186:Metagenomics; D009659:North Sea; D050741:Phocoena / Q000821:virology*; D010802:Phylogeny