title: A structural appraisal of sterol carrier protein 2.
abstract: Sterol Carrier Protein 2 (SCP2) has been associated with lipid binding and transfer activities. However, genomic, proteomic, and structural studies revealed that it is an ubiquitous domain of complex proteins with a variety functions in all forms of life. High-resolution structures of representative SCP2 domains are available, encouraging a comprehensive review of the structural basis for its success. Most SCP2 domains pertain to three major families and are frequently found as stand-alone or at the C-termini of lipid related peroxisomal enzymes, acetyltransferases causing bacterial resistance, and bacterial environmentally important sulfatases. We (1) analyzed the structural basis of the fold and the classification of SCP2 domains; (2) identified structure-determined sequence features; (3) compared the lipid binding cavity of SCP2 and other lipid binding proteins; (4) surveyed proposed mechanisms of SCP2 mediated lipid transfer between membranes; and (5) uncovered a possible new function of the SCP2 domain as a protein-protein recognition device.
authors.affiliation: Instituto de Química y Fisicoquímica Biológicas, UBA, CONICET, FFyB, Argentina.
authors.forename: Noelia I
authors.initials: NI
authors.lastname: Burgardt
authors.affiliation: Grupo Vinculado de Biología Estructural y Biotecnología, Universidad Nacional de Quilmes, IMBICE, CIC, UNLP, CONICET, Argentina.
authors.forename: Alejo R
authors.initials: AR
authors.lastname: Gianotti
authors.affiliation: Grupo Vinculado de Biología Estructural y Biotecnología, Universidad Nacional de Quilmes, IMBICE, CIC, UNLP, CONICET, Argentina.
authors.forename: Raúl G
authors.initials: RG
authors.lastname: Ferreyra
authors.affiliation: Grupo Vinculado de Biología Estructural y Biotecnología, Universidad Nacional de Quilmes, IMBICE, CIC, UNLP, CONICET, Argentina. Electronic address: ermacora@unq.edu.ar.
authors.forename: Mario R
authors.initials: MR
authors.lastname: Ermácora
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D020675:Peroxisomes / Q000201:enzymology; D011485:Protein Binding; D000072417:Protein Domains; D017510:Protein Folding; D060066:Protein Interaction Maps; D040901:Proteomics; D013261:Sterols / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D008055:Lipids; D013261:Sterols; C072087:sterol carrier proteins
keywords: Alkyl sulfatase; Enhanced intracellular survival protein; Lipid binding protein; MycE methyltransferase; Non-specific lipid-transfer protein; Sterol carrier protein 2