title: Naphthacemycins, novel circumventors of β-lactam resistance in MRSA, produced by Streptomyces sp. KB-3346-5. I. The taxonomy of the producing strain, and the fermentation, isolation and antibacterial activities.
abstract: Screening for circumventors of β-lactam resistance in methicillin-resistant Staphylococcus aureus (MRSA) led us to find 17 novel antibiotics, naphthacemycins A1-A11, B1-B4and C1-C2. The naphthacemycins were isolated from a cultured broth of Streptomyces sp. KB-3346-5 by repeated silica gel column chromatography and HPLC. Naphthacemycins enhanced imipenem activity 100-500 times against MRSA at 0.5 μg ml-1, and naphthacemycins A4-A11themselves showed MIC50values of 1-4 μg ml-1against 22 MRSA strains.
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukumoto
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yong-Pil
authors.initials: YP
authors.lastname: Kim
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Healthcare Products Development Center, Kyowa Hakko Bio Co., Ltd, Ibaraki, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Chemical Research Laboratories, Kyowa Hakko Kirin Co., Ltd, Shizuoka, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Onodera
authors.affiliation: School of Pharmacy, Kitasato University, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Hidehito
authors.initials: H
authors.lastname: Matsui
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hanaki
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ōmura
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.|Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D015195:Drug Design; D004357:Drug Synergism; D015378:Imipenem / Q000008:administration & dosage / Q000494:pharmacology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*; D018440:beta-Lactam Resistance; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D015378:Imipenem