title: Subchronic toxicity and anti-HSV-1 activity in experimental animal of dolabelladienetriol from the seaweed, Dictyota pfaffii.
abstract: This study examined in rats the subchronic toxicity and anti- HSV-1activity after oral administration of dolabelladienetriol (D1), a diterpene isolated from the seaweed Dictyota pfaffii. In subchronic toxicity (SCT) tests, female rats received D1 by gavage 15 mg/kg/day (n = 5) for 50 days, and general behavior, death, hematological, biochemical and histological changes in the liver, kidney, stomach, and duodenum were determined. For the anti-HSV-1 activity, female mice were infected and treated orally with a dose of 20 mg/kg (n = 5) twice a day with D1 and any lesions in the skin were then recorded for 18 days. Dolabelladienetriol in SCT did not significantly change behavior, body weight, hematological or biochemical profiles. The liver and kidneys, however, showed some alterations in rats treated with D1, similar to those in rats treated with ACV, while the other tissues had no significant changes. The anti-HSV-1 activity of D1 had a similar efficacy to the ACV drug control in mice. Our results showed that D1 has potential commercial development as a new HSV-1drug.
authors.affiliation: Department of Cellular and Molecular Biology, Laboratory of Molecular Virology Molecular - Biology Institute, Federal Fluminense University (UFF), Science and Biotechnology Postgraduate Program, Campos do Valonguinho, sn, Niterói, RJ, 24020-150, Brazil. Electronic address: valgarrido@gmail.com.
authors.forename: Valéria
authors.initials: V
authors.lastname: Garrido
authors.affiliation: Department of Cellular and Molecular Biology, Laboratory of Molecular Virology Molecular - Biology Institute, Federal Fluminense University (UFF), Science and Biotechnology Postgraduate Program, Campos do Valonguinho, sn, Niterói, RJ, 24020-150, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Barros
authors.affiliation: Department of Cellular and Molecular Biology, Laboratory of Molecular Virology Molecular - Biology Institute, Federal Fluminense University (UFF), Science and Biotechnology Postgraduate Program, Campos do Valonguinho, sn, Niterói, RJ, 24020-150, Brazil.
authors.forename: Vanessa A
authors.initials: VA
authors.lastname: Melchiades
authors.affiliation: ALGAMAR-Laboratory Marine Biology Department, Institute of Biology of the UFF, Brazil.
authors.forename: Rainomar Raimundo
authors.initials: RR
authors.lastname: Fonseca
authors.affiliation: Department of Chemistry Organic, Institute of Chemistry of the UFF, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Pinheiro
authors.affiliation: General Pathology, Microbiology and Parasitology Department, Biomedical Institute, Federal University of Rio de Janeiro State/Laboratory of Innovations in Therapies, Essays and Bio Products. de, Oswaldo Cruz Institute/FIOCRUZ, Av. Brasil 4365, Manguinhos, Pavilhão Cardoso Fontes, 65, Rio de Janeiro, RJ, 21045-900, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Ocampo
authors.affiliation: ALGAMAR-Laboratory Marine Biology Department, Institute of Biology of the UFF, Brazil.
authors.forename: Valéria L
authors.initials: VL
authors.lastname: Teixeira
authors.affiliation: ALGAMAR-Laboratory Marine Biology Department, Institute of Biology of the UFF, Brazil.
authors.forename: Diana N
authors.initials: DN
authors.lastname: Cavalcanti
authors.affiliation: Department of Cellular and Molecular Biology, Laboratory of Molecular Virology Molecular - Biology Institute, Federal Fluminense University (UFF), Science and Biotechnology Postgraduate Program, Campos do Valonguinho, sn, Niterói, RJ, 24020-150, Brazil.
authors.forename: Viveca
authors.initials: V
authors.lastname: Giongo
authors.affiliation: Department of Biosciences, College of Science, Swansea University, Singleton Park, Swansea SA2 8PP, UK.
authors.forename: Norman A
authors.initials: NA
authors.lastname: Ratcliffe
authors.affiliation: Department of Immunobiology, Biology Institute, Federal Fluminense University, Campus do Valonguinho, sn, Niterói, RJ, 24020-150, Brazil.
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Teixeira
authors.affiliation: Department of Cellular and Molecular Biology, Laboratory of Molecular Virology Molecular - Biology Institute, Federal Fluminense University (UFF), Science and Biotechnology Postgraduate Program, Campos do Valonguinho, sn, Niterói, RJ, 24020-150, Brazil. Electronic address: Izabeluff@gmail.com.
authors.forename: Izabel Christina N P
authors.initials: ICNP
authors.lastname: Paixão
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D000998:Antiviral Agents / Q000633:toxicity*; D005260:Female; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D012624:Seaweed / Q000737:chemistry*; D059550:Toxicity Tests, Subchronic
chemical_list: D000998:Antiviral Agents
keywords: Anti-HSV-1 activity; BALB/c mice; Dictyota pfaffii; Dolabelladienetriol; Subchronic toxicity; Wistar rats