title: Regulation of Respiratory Pathway and Electron Transport Chain in Relation to Senescence of Postharvest White Mushroom (Agaricus bisporus) under High O2/CO2Controlled Atmospheres.
abstract: In order to study the respiration metabolism mechanism based on the generation of adenosine triphosphate (ATP) and reactive oxygen species (ROS) and nitric oxide (NO) by the electron transport chain (ETC) of the white mushroom under high O2/CO2controlled atmospheres, the treatments of 100% O2, 80% O2+ 20% CO2, 60% O2+ 40% CO2, and 40% O2+ 60% CO2at 2 ± 1 °C were employed and natural air was used as the control. ATP and energy charge can maintain the membrane integrity and function, life activities, and physicochemical reactions of higher plants. The results showed that the 80% O2+ 20% CO2treatment inhibited the respiration rate, embden-meyerhof-parnas or glycolysis pathway, and ROS and NO contents. It significantly delayed the reduction of the ATP content and energy charge level, tricarboxyfic-acid-cycle and cytochrome pathway proportion, and their key enzymes activity and gene expression. It also maintained a high phosphopentose pathway and moderate alternative pathway. Results indicated that the 80% O2+ 20% CO2prolonged the storage time of mushrooms to 24 days and retarded the senescence through retaining the higher energy, suppressing the ROS contents, enhancing the endurance capability in adversity, and regulating the respiration pathways and ETC metabolism.
authors.affiliation: School of Agricultural and Food Engineering, Shandong University of Technology , Zibo 255049, People's Republic of China.|Food Research Institute, National Agriculture and Food Research Organization, Tsukuba , Ibaraki 305-8642, Japan.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Food Research Institute, National Agriculture and Food Research Organization, Tsukuba , Ibaraki 305-8642, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kitazawa
authors.affiliation: School of Agricultural and Food Engineering, Shandong University of Technology , Zibo 255049, People's Republic of China.
authors.forename: Xiangyou
authors.identifier: http://orcid.org/0000-0003-4918-0893
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Agricultural and Food Engineering, Shandong University of Technology , Zibo 255049, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Sun
mesh_terms: D000364:Agaricus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D001272:Atmosphere; D002245:Carbon Dioxide / Q000032:analysis / Q000378:metabolism*; D004579:Electron Transport; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014675:Vegetables / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D002245:Carbon Dioxide; D010100:Oxygen
keywords: Agaricus bisporus; electron transport chain; energy; high O2; respiration pathway