title: Murine Cytomegalovirus Infection Induces Susceptibility to EAE in Resistant BALB/c Mice.
abstract: In contrast to C57BL/6 mice, BALB/c mice are relatively resistant to the induction of experimental autoimmune encephalomyelitis (EAE) after challenge with MOG35-55peptide. Here, we provide the first evidence that infection with murine cytomegalovirus (MCMV) in adulthood abrogates this resistance. Infected BALB/c mice developed clinical and histological signs similar to those seen in susceptible C57BL/6 mice. In addition to CD4+cells, large proportion of cells in the infiltrate of diseased BALB/c mice was CD8+, similar with findings in multiple sclerosis. CD8+cells that responded toex vivorestimulation with MOG35-55were not specific for viral epitopes pp89 and m164. MCMV infection favors proinflammatory type of dendritic cells (CD86+CD40+CD11c+) in the peripheral lymph organs, M1 type of microglia in central nervous system, and increases development of Th1/Th17 encephalitogenic cells. This study indicates that MCMV may enhance autoimmune neuropathology and abrogate inherent resistance to EAE in mouse strain by enhancing proinflammatory phenotype of antigen-presenting cells, Th1/Th17, and CD8 response to MOG35-55.
authors.affiliation: Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia; Faculty of Medical Sciences, Institute of Histology, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Milovanovic
authors.affiliation: Center for Proteomics, Faculty of Medicine, Department for Histology and Embryology, University of Rijeka , Rijeka , Croatia.
authors.forename: Branka
authors.initials: B
authors.lastname: Popovic
authors.affiliation: Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Milovanovic
authors.affiliation: Center for Proteomics, Faculty of Medicine, Department for Histology and Embryology, University of Rijeka , Rijeka , Croatia.
authors.forename: Daria
authors.initials: D
authors.lastname: Kvestak
authors.affiliation: Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Arsenijevic
authors.affiliation: Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia; Faculty of Medical Sciences, Institute of Pathophysiology, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Stojanovic
authors.affiliation: Faculty of Medical Sciences, Institute of Histology, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Irena
authors.initials: I
authors.lastname: Tanaskovic
authors.affiliation: Center for Proteomics, Faculty of Medicine, Department for Histology and Embryology, University of Rijeka , Rijeka , Croatia.
authors.forename: Astrid
authors.initials: A
authors.lastname: Krmpotic
authors.affiliation: Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Arsenijevic
authors.affiliation: Center for Proteomics, Faculty of Medicine, Department for Histology and Embryology, University of Rijeka , Rijeka , Croatia.
authors.forename: Stipan
authors.initials: S
authors.lastname: Jonjic
authors.affiliation: Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Miodrag L
authors.initials: ML
authors.lastname: Lukic
keywords: BALB/c mice; T cells; antigen-presenting cells; experimental autoimmune encephalomyelitis; murine cytomegalovirus infection