title: A High-Throughput Screening Platform of Microbial Natural Products for the Discovery of Molecules with Antibiofilm Properties againstSalmonella.
abstract: In this report, we describe a High-Throughput Screening (HTS) to identify compounds that inhibit biofilm formation or cause the disintegration of an already formed biofilm using theSalmonellaEnteritidis 3934 strain. Initially, we developed a new methodology for growingSalmonellabiofilms suitable for HTS platforms. The biomass associated with biofilm at the solid-liquid interface was quantified by staining both with resazurin and crystal violet, to detect living cells and total biofilm mass, respectively. For a pilot project, a subset of 1120 extracts from the Fundación MEDINA's collection was examined to identify molecules with antibiofilm activity. This is the first validated HTS assay of microbial natural product extracts which allows for the detection of four types of activities which are not mutually exclusive: inhibition of biofilm formation, detachment of the preformed biofilm and antimicrobial activity against planktonic cells or biofilm embedded cells. Currently, several extracts have been selected for further fractionation and purification of the active compounds. In one of the natural extracts patulin has been identified as a potent molecule with antimicrobial activity against both, planktonic cells and cells within the biofilm. These findings provide a proof of concept that the developed HTS can lead to the discovery of new natural compounds with antibiofilm activity againstSalmonellaand its possible use as an alternative to antimicrobial therapies and traditional disinfectants.
authors.affiliation: Department de Genètica, Microbiologia i Estadística, Facultat de Biologia, Universitat de Barcelona Barcelona, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Paytubi
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: de La Cruz
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Jose R
authors.initials: JR
authors.lastname: Tormo
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Martín
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: González
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Victor
authors.initials: V
authors.lastname: González-Menendez
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Genilloud
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Reyes
authors.affiliation: Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud Granada, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Vicente
authors.affiliation: Department de Genètica, Microbiologia i Estadística, Facultat de Biologia, Universitat de Barcelona Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Madrid
authors.affiliation: Department de Genètica, Microbiologia i Estadística, Facultat de Biologia, Universitat de Barcelona Barcelona, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Balsalobre
keywords: HTS; Salmonella; antimicrobial; biofilm; natural products; patulin