title: Effects of Roundup formulations on biochemical biomarkers and male sperm quality of the livebearing Jenynsia multidentata.
abstract: Roundup®formulations are the most consumed glyphosate-based herbicides in the world. When applied, they may reach water bodies and exert toxicity toward non-target species. This study evaluated and compared the effects of different variations of Roundup on biochemical biomarkers as oxidative parameters and Acethylchorinesterase (AChE) activity, and sperm quality of the livebearing Jenynsia multidentata. Fish were acutely (96 h) exposed to Roundup Original®(RO), Roundup Transorb®(RT) and Roundup WG®(RWG) at 0.0, 0.5, 1 and 5 mg L-1of nominal glyphosate. The highest mortality (60%) was observed for fish exposed to RT at the highest concentration tested and at 0.5 mg L-1non-mortality was observed, so this concentration was chosen for the experiments. Fish exposed to RO and RT (24 and 96 h) presented a state of oxidative imbalance, which caused lipid peroxidation (LPO) in their livers. Oxidative stress was more severe in RO treatment, which may be resulted in the highest hepathosomatic index at 96 h. However, fish exposed to RT presented a marked inhibition of AChE activity from membrane cells of muscle and brain tissues. Sperm quality was investigated in livebearing exposed (24 and 96 h) to the three formulations. Spermatozoa motility and concentration were affected by all formulations. Overall, Roundup formulations are harmful to the fish J. multidentata at 0.5 mg L-1of glyphosate; however, mechanisms and potential of toxicity are different between formulations. The J. multidentata also represents a sensitive species and a good regional bio-monitor.
authors.affiliation: Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
authors.forename: Jessica Andrea Albañil
authors.initials: JA
authors.lastname: Sánchez
authors.affiliation: Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil; Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
authors.forename: Antonio Sergio
authors.initials: AS
authors.lastname: Varela
authors.affiliation: Departamento de Patologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Caixa Postal 354, 96001-970, Pelotas, RS, Brazil.
authors.forename: Carine Dahl
authors.initials: CD
authors.lastname: Corcini
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
authors.forename: Janaina Camacho
authors.initials: JC
authors.lastname: da Silva
authors.affiliation: Escola de Química e Alimentos, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
authors.forename: Ednei Gilberto
authors.initials: EG
authors.lastname: Primel
authors.affiliation: Escola de Química e Alimentos, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
authors.forename: Sergiane
authors.initials: S
authors.lastname: Caldas
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
authors.forename: Roberta Daniele
authors.initials: RD
authors.lastname: Klein
authors.affiliation: Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil; Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil. Electronic address: camilaos@hotmail.com.
authors.forename: Camila De Martinez Gaspar
authors.initials: CM
authors.lastname: Martins
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry*; D003532:Cyprinodontiformes; D005998:Glycine / Q000031:analogs & derivatives / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
chemical_list: D015415:Biomarkers; D005998:Glycine; D006540:Herbicides; D014874:Water Pollutants, Chemical; D000097797:Glyphosate
keywords: Acethylchorinesterase; Biomarker; Fish; Glyphosate; Oxidatve stress; Sperm quality