title: Secondary Metabolites from the Marine-Derived Fungus Dichotomomyces sp. L-8 and Their Cytotoxic Activity.
abstract: Bioassay-guided isolation of the secondary metabolites from the fungusDichotomomyces sp. L-8 associated with the soft coralLobophytum crassumled to the discovery of two new compounds, dichotones A and B (1and2), together with four known compounds including dichotocejpin C (3), bis-N-norgliovictin (4), bassiatin (5) and (3R,6R)-bassiatin (6). The structures of these compounds were determined by 1D, 2D NMR and mass spectrometry. (3R,6R)-bassiatin (6) displayed significant cytotoxic activities against the human breast cancer cell line MDA-MB-435 and the human lung cancer cell line Calu3 with IC50values of 7.34 ± 0.20 and 14.54 ± 0.01 μM, respectively, while bassiatin (5), the diastereomer of compound6, was not cytotoxic.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. huanglh23@mail2.sysu.edu.cn.|Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China. huanglh23@mail2.sysu.edu.cn.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. chenyx239@mail2.sysu.edu.cn.|Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China. chenyx239@mail2.sysu.edu.cn.
authors.forename: Yan-Xiu
authors.initials: YX
authors.lastname: Chen
authors.affiliation: Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, China. lisana121406@163.com.
authors.forename: Jian-Chen
authors.initials: JC
authors.lastname: Yu
authors.affiliation: Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, China. yuanjie@mail.sysu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.affiliation: School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China. ceslhj@mail.sysu.edu.cn.
authors.forename: Hou-Jin
authors.initials: HJ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Avenida Wai Long, Taipa 519020, Macau (SAR), China. wzma@must.edu.mo.
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Ma
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. ramidawa@yahoo.com.
authors.forename: Ramida
authors.initials: R
authors.lastname: Watanapokasin
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. Hukch@mail2.sysu.edu.cn.|Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China. Hukch@mail2.sysu.edu.cn.
authors.forename: Kun-Chao
authors.initials: KC
authors.lastname: Hu
authors.affiliation: Institute of Chemical Sciences, Gomal University, D.I.Khan 29050, Pakistan. shah_iram2000@Yahoo.com.
authors.forename: Shah Iram
authors.initials: SI
authors.lastname: Niaz
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. lssydp@mail.sysu.edu.cn.|Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China. lssydp@mail.sysu.edu.cn.
authors.forename: De-Po
authors.initials: DP
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. lanwj@mail.sysu.edu.cn.|Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China. lanwj@mail.sysu.edu.cn.
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Lan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D059001:Aquatic Organisms; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D054659:Diketopiperazines / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009025:Morpholines / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004718:Saccharomycetales / Q000737:chemistry / Q000378:metabolism*; D064210:Secondary Metabolism / Q000502:physiology*; D013329:Structure-Activity Relationship; D013440:Sulfides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D054659:Diketopiperazines; D009025:Morpholines; D013440:Sulfides; C584130:bis-N-norgliovictin; C085610:lateritin
keywords: Dichotomomyces sp.; cytotoxic activity; secondary metabolites; structure elucidation