title: Reassessing Escherichia coli as a cell factory for biofuel production.
abstract: Via metabolic engineering, industrial microorganisms have the potential to convert renewable substrates into a wide range of biofuels that can address energy security and environmental challenges associated with current fossil fuels. The user-friendly bacterium, Escherichia coli, remains one of the most frequently used hosts for demonstrating production of biofuel candidates including alcohol-, fatty acid- and terpenoid-based biofuels. In this review, we summarize the metabolic pathways for synthesis of these biofuels and assess enabling technologies that assist in regulating biofuel synthesis pathways and rapidly assembling novel E. coli strains. These advances maintain E. coli's position as a prominent host for developing cell factories for biofuel production.
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors.forename: Chonglong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: brian.pfleger@wisc.edu.
authors.forename: Brian F
authors.initials: BF
authors.lastname: Pfleger
authors.affiliation: Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea. Electronic address: swkim@gnu.ac.kr.
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
mesh_terms: D056804:Biofuels; D004926:Escherichia coli / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways
chemical_list: D056804:Biofuels; D000431:Ethanol