title: Calcium-dependent protein kinase CPK31 interacts with arsenic transporter AtNIP1;1 and regulates arsenite uptake in Arabidopsis thaliana.
abstract: Although arsenite [As(III)] is non-essential and toxic for plants, it is effectively absorbed through various transporters into the roots. Here we identified a calcium-dependent protein kinase (CPK31) response for As(III) tolerance in Arabidopsis. We identified CPK31 as an interacting protein of a nodulin 26-like intrinsic protein (NIP1;1), an aquaporin involved in As(III) uptake. Similarly to the nip1;1 mutants, the loss-of-function mutants of CPK31 improved the tolerance against As(III) but not As(V), and accumulated less As(III) in roots than that of the wild-type plants. The promoter-β-glucuronidase and quantitative Real-Time PCR analysis revealed that CPK31 displayed overlapping expression profiles with NIP1;1 in the roots, suggesting that they might function together in roots. Indeed, the cpk31 nip1;1 double mutants exhibited stronger As(III) tolerance than cpk31 mutants, but similar to nip1;1 mutants, supporting the idea that CPK31 might serve as an upstream regulator of NIP1;1. Furthermore, transient CPK31 overexpression induced by dexamethasone caused the decrease in As(III) tolerance of transgenic Arabidopsis lines. These findings reveal that CPK31 is a key factor in As(III) response in plants.
authors.affiliation: College of Resources and Environmental Science, Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Ji
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry University, Fujian, Fuzhou, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Jinglong
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Resources and Environmental Science, Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Qinsong
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Haiman
authors.initials: H
authors.lastname: Ge
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Fugeng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, CA, United States of America.
authors.forename: Sheng
authors.initials: S
authors.lastname: Luan
authors.affiliation: State Key Laboratory for Pharmaceutical Biotechnology, Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Lan
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D002352:Carrier Proteins; D011494:Protein Kinases; D001151:Arsenic