title: PEGylation corannulene enhances response of stress through promoting neurogenesis.
abstract: Carbon nanomaterials have been used to treat neurodegenerative diseases and neural disorders due to their diverse molecular structures. Corannulene is a three-dimensional π-bowl carbon nanomaterial that is different from planar PAHs, fullerenes and carbon nanotubes, but little is known about its biological functions. Herein, corannulene was functionalized with mPEG-DESP to prepare PEGylation corannulene nanoparticles (PEGylation CoNps). The synthesized PEGylation CoNps shows enhanced solubility and reduced aggregation when compared corannulene. Then, in vivo experiments were performed to determine the effects of PEGylation CoNps on the neural system. We found that PEGylation CoNps treatment increased short resting bouts, decreased locomotion activities and enhanced the response to stress. Most of these behavioral changes suggest that PEGylation CoNps lead to a greater reflection to stress, which is associated with neurotransmitter expression and neurogenesis. In line with the hypothesis, we found that PEGylation CoNps administration enhanced TH, DCX and MAP-2 expression in the hippocampus. These results indicated that PEGylation CoNps enhanced the neurogenesis of mice. Furthermore, pathological analysis showed that PEGylation CoNps caused little inflammation. These findings suggest that PEGylation CoNps are a potential functionalized carbon nanomaterial for promoting neurogenesis.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Li
authors.affiliation: The Institute of Robotics and Automatic Information Systems, Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Sun
authors.affiliation: Tianjin Key Laboratory of Tumour Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of Medicine, Nankai University, Tianjin 300071, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Dashuai
authors.initials: D
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Deling
authors.identifier: http://orcid.org/0000-0002-2961-9267
authors.initials: D
authors.lastname: Kong
authors.affiliation: The Institute of Robotics and Automatic Information Systems, Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: The Institute of Robotics and Automatic Information Systems, Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory of Tumour Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of Medicine, Nankai University, Tianjin 300071, China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn.
authors.forename: Xizeng
authors.identifier: http://orcid.org/0000-0003-1089-1161
authors.initials: X
authors.lastname: Feng
mesh_terms: D000818:Animals; D000089662:Doublecortin Protein; D005260:Female; D008124:Locomotion / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055495:Neurogenesis / Q000187:drug effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry* / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein; D011084:Polycyclic Aromatic Hydrocarbons; C434863:corannulene; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol