title: Anti-HLA-DQ antibodies are highly and independently related to the C1q-binding capacity of HLA antibodies.
abstract: The complement-binding capacity of anti-HLA antibodies (HLAabs) is recognized as a key pathogenic factor. The aim of this study is to describe the patient characteristics associated to the presence of C1q+ as well as those of the Abs per se when associated to C1q binding.
  
  
  
  This is a cross-sectional, observational, descriptive study of patients with previous sensitizing factors and awaiting a kidney transplant (KT). We determined anti-HLA antibodies and their C1q binding capacity with the C1q assay.
  
  
  
  Among the 55 included patients, 26 (47.2%) had at least one C1q+ anti-HLAab. A previous transplant history, a greater number of HLAabs, a greater % of class I or class II PRA, the average MFI of all HLAabs, the MFI of the dominant HLAab and the HLAab antigenic specificities against HLA-B, -C and -DQ, all proved to be risk factors associated to the presence of C1q binding HLAabs (C1q+). In the total population, were detected 1268 HLAabs, 230 (18.1%) of which were C1q+. On multivariate analysis, both HLAabs against the HLA-DQ antigenic specificity (OR 9.82 95% CI 5.4-17.6, p<0.001) and the MFI documented by LABScreen®SAB (OR 1.2% CI 1.22-1.3, p<0.001), proved to be risk factors.
  
  
  
  Anti-HLA-DQ antibodies and the MFI (LABScreen®SAB) are highly and independently related to the C1q-binding capacity of HLA antibodies.
authors.affiliation: Department of Transplantation, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: José M
authors.initials: JM
authors.lastname: Arreola-Guerra
authors.affiliation: Department of Nephrology-Mineral Metabolism, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Morales-Buenrostro
authors.affiliation: Department of Transplantation, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Julio
authors.initials: J
authors.lastname: Granados
authors.affiliation: Histocompatibility Laboratory, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Castelán
authors.affiliation: Histocompatibility Laboratory, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Adrián
authors.initials: A
authors.lastname: de Santiago
authors.affiliation: Histocompatibility Laboratory, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Adriana
authors.initials: A
authors.lastname: Arvizu
authors.affiliation: Histocompatibility Laboratory, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Norma
authors.initials: N
authors.lastname: Gonzalez-Tableros
authors.affiliation: Histocompatibility Laboratory, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Mayra
authors.initials: M
authors.lastname: López
authors.affiliation: Department of Transplantation, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Vilatobá
authors.affiliation: Department of Transplantation, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, Mexico City 14080, Mexico. Electronic address: josefinaalberu@hotmail.com.
authors.forename: Josefina
authors.initials: J
authors.lastname: Alberú
mesh_terms: D000328:Adult; D015922:Complement C1q / Q000276:immunology*; D003430:Cross-Sectional Studies; D005260:Female; D006683:HLA-DQ Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged
chemical_list: D006683:HLA-DQ Antigens; D007518:Isoantibodies; D015922:Complement C1q