title: Synergistic Degradation of a Hyperuricemia-Causing Metabolite Using One-Pot Enzyme-Nanozyme Cascade Reactions.
abstract: Multi-enzyme cascade reactions are frequently found in living organisms, in particular when an intermediate should be eliminated. Recently, enzyme-mimic nanomaterials (nanozymes) received much attention for various applications, because they are usually more stable and cost-effective than enzymes. However, enzyme-nanozyme cascade reations have not been yet extensively exploited. Therefore, in this study, we investigated one-pot enzyme-nanozyme cascade reactions using urate oxidase (UOX) and catalase-mimic gold nanoparticle nanozyme (AuNP) with the ultimate goal of treatment of hyperuricemia. UOX degrades hyperuricemia-causing uric acid, but also generates hydrogen peroxide raising several health concerns. We successfully demonstrated that one-pot UOX-AuNP cascade systems degrade uric acid more than five times faster than UOX alone, by eliminating potentially cytotoxic hydrogen peroxide, similar to enzyme-enzyme reactions.
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
authors.forename: Secheon
authors.initials: S
authors.lastname: Jung
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
authors.forename: Inchan
authors.initials: I
authors.lastname: Kwon
mesh_terms: D002374:Catalase / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D033461:Hyperuricemia / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D013053:Spectrophotometry; D014503:Urate Oxidase / Q000378:metabolism*; D014527:Uric Acid / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D014527:Uric Acid; D006046:Gold; D006861:Hydrogen Peroxide; D002374:Catalase; D014503:Urate Oxidase