title: The Hydrogen Sulfide Releasing Molecule Acetyl Deacylasadisulfide Inhibits Metastatic Melanoma.
abstract: Melanoma is the most common form of skin cancer. Given its high mortality, the interest in the search of preventive measures, such as dietary factors, is growing significantly. In this study we tested,in vitroandin vivo, the potential anti-cancer effect of the acetyl deacylasadisulfide (ADA), a vinyl disulfide compound, isolated and purified from asafoetida a foul-smelling oleo gum-resin of dietary and medicinal relevance. ADA markedly suppressed proliferation of human melanoma cell lines by inducing apoptosis. Moreover, treatment of melanoma cells with ADA reduced nuclear translocation and activation of NF-κB, decreased the expression of the anti-apoptotic proteins c-FLIP, XIAP, and Bcl-2 and inhibited the phosphorylation and activation of both AKT and ERK proteins, two of the most frequently deregulated pathways in melanoma. Finally, the results obtainedin vitrowere substantiated by the findings that ADA significantly and dose-dependently reduced lung metastatic foci formation in C57BL/6 mice. In conclusion, our findings suggest that ADA significantly inhibits melanoma progressionin vivoand could represent an important lead compound for the development of new anti-metastatic agents.
authors.affiliation: Department of Pharmacy, University of Naples Federico II Naples, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: De Cicco
authors.affiliation: Department of Pharmacy, University of Naples Federico II Naples, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Panza
authors.affiliation: Department of Pharmacy, University of Naples Federico II Naples, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Armogida
authors.affiliation: Department of Pharmacy, University of Naples Federico II Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ercolano
authors.affiliation: Department of Pharmacy, University of Naples Federico II Naples, Italy.
authors.forename: Orazio
authors.initials: O
authors.lastname: Taglialatela-Scafati
authors.affiliation: Department of Pharmacognosy and Biotechnology, School of Pharmacy, Kermanshah University of Medical Sciences Kermanshah, Iran.
authors.forename: Yalda
authors.initials: Y
authors.lastname: Shokoohinia
authors.affiliation: Department of Experimental Oncology, National Cancer Institute -IRCCS "G.Pascale" Foundation Naples, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Camerlingo
authors.affiliation: Department of Experimental Oncology, National Cancer Institute -IRCCS "G.Pascale" Foundation Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pirozzi
authors.affiliation: Department of Pharmacy, University of Pisa Pisa, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Calderone
authors.affiliation: Department of Pharmacy, University of Naples Federico II Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cirino
authors.affiliation: Department of Pharmacy, University of Naples Federico II Naples, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Ianaro
keywords: apoptosis; hydrogen sulfide; melanoma; metastasis; skin cancer