title: A salt-stress-regulator from the Poplar R2R3 MYB family integrates the regulation of lateral root emergence and ABA signaling to mediate salt stress tolerance in Arabidopsis.
abstract: The roles of most MYB transcription factors (TFs) in the poplar remain unclear. Here, we demonstrate that PtrSSR1, a salt-stress-regulator in the Populus trichocarpa R2R3 MYB gene family, mediates the tolerance of transgenic Arabidopsis plants to salt stress. The transcripts of PtrSSR1 could be induced by salt stress rapidly in poplar. Subcellular localization and yeast assays indicated that PtrSSR1 encoded a nuclear protein with transactivation activity. The Arabidopsis transformants overexpressing PtrSSR1 clearly displayed lateral root emergence (LRE) inhibition compared with wild-type (Wt) under normal conditions; while upon NaCl treatment, the transformants showed improved tolerance, and the LRs emerged faster from salt-induced inhibition. A strong correlation could exist between the LRE mediated by PtrSSR1 and abscisic acid (ABA), mainly because the transformants displayed more sensitivity to exogenous ABA during both seed germination and LRE, and had a distinctly increased level of endogenous ABA. Furthermore, several ABA- and salt-related genes, such as NCED3, ABI1 and CBL1, were up-regulated. Thus, our results suggest that elevation in the endogenous ABA content bring alteration of plant LR development, and that the poplar R2R3 MYB TF PtrSSR1 vitally improve salt stress tolerance by integrating the regulation of LRE and ABA signaling in Arabidopsis.
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China; Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China. Electronic address: fangqing2005@126.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Tianzhi
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Liangxiang
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Mao
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Xianqiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Yanjiao
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Guozheng
authors.initials: G
authors.lastname: Tian
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, School of Biological Science and Technology, Enshi 445000, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Di
authors.initials: D
authors.lastname: Fan
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China.
authors.forename: Bangjun
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education Chongqing, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address: kemingl@swu.edu.cn.
authors.forename: Keming
authors.initials: K
authors.lastname: Luo
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D032107:Populus / Q000187:drug effects / Q000502:physiology*; D055049:Salt Tolerance / Q000502:physiology*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: ABA; Lateral root; MYB transcription factor; Poplar; Salt stress