title: Hierarchical templating in deposition of semi-covalently imprinted inverse opal polythiophene film for femtomolar determination of human serum albumin.
abstract: Nanostructured artificial receptor materials with unprecedented hierarchical structure for determination of human serum albumin (HSA) are designed and fabricated. For that purpose a new hierarchical template is prepared. This template allowed for simultaneous structural control of the deposited molecularly imprinted polymer (MIP) film on three length scales. A colloidal crystal templating with optimized electrochemical polymerization of 2,3'-bithiophene enables deposition of an MIP film in the form of an inverse opal. Thickness of the deposited polymer film is precisely controlled with the number of current oscillations during potentiostatic deposition of the imprinted poly(2,3'-bithiophene) film. Prior immobilization of HSA on the colloidal crystal allows formation of molecularly imprinted cavities exclusively on the internal surface of the pores. Furthermore, all binding sites are located on the surface of the imprinted cavities at locations corresponding to positions of functional groups present on the surface of HSA molecules due to prior derivatization of HSA molecules with appropriate functional monomers. This synergistic strategy results in a material with superior recognition performance. Integration of the MIP film as a recognition unit with a sensitive extended-gate field-effect transistor (EG-FET) transducer leads to highly selective HSA determination in the femtomolar concentration range.
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Dabrowski
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. Electronic address: mcieplak@ichf.edu.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Cieplak
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Piyush Sindhu
authors.initials: PS
authors.lastname: Sharma
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Borowicz
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Noworyta
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Lisowski
authors.affiliation: Department of Chemistry, University of North Texas, 1155 Union Circle No. 305070, Denton, TX 76203-5017, United States. Electronic address: francis.dsouza@unt.edu.
authors.forename: Francis
authors.initials: F
authors.lastname: D'Souza
authors.affiliation: Univ. Bordeaux, CNRS UMR 5255, Bordeaux INP, ENSCBP, 16 Avenue Pey Berland, 33607 Pessac, France. Electronic address: kuhn@enscbp.fr.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kuhn
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland; Faculty of Mathematics and Natural Sciences, School of Sciences, Cardinal Stefan Wyszynski University in Warsaw, Woycickiego 1/3, 01-815 Warsaw, Poland. Electronic address: wkutner@ichf.edu.pl.
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Kutner
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D000075462:Serum Albumin, Human / Q000302:isolation & purification*; D013876:Thiophenes / Q000737:chemistry
chemical_list: D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D000075462:Serum Albumin, Human
keywords: Extended-gate field-effect transistor (EG-FET); Human serum albumin (HSA); Inverse opals; Protein imprinting; Semi-covalent imprinting; Surface imprinting