title: Precise Photoremovable Perturbation of a Virus-Host Interaction.
abstract: Viruses utilize distinct binding interactions with a variety of host factors to gain entry into host cells. A chemical strategy is described to precisely perturb a specific molecular interaction between adeno-associated virus and its host cell, which can be rapidly reversed by light. This strategy enables pausing the virus entry process at a specific stage and then restart it rapidly with a non-invasive stimulus. The ability to synchronize the invading virus population at a discrete step in its entry pathway will be highly valuable for enabling facile experimental characterization of the molecular processes underlying this process. Additionally, adeno-associated virus has demonstrated outstanding potential for human gene therapy. This work further provides a potential approach to create therapeutic vectors that can be photoactivated in vivo with high spatial and temporal control.
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Sarah B
authors.initials: SB
authors.lastname: Erickson
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Raja
authors.initials: R
authors.lastname: Mukherjee
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Kelemen
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Chester J J
authors.initials: CJ
authors.lastname: Wrobel
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Xiaofu
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.
authors.forename: Abhishek
authors.identifier: 0000-0002-6231-5302
authors.initials: A
authors.lastname: Chatterjee
mesh_terms: D000229:Dependovirus / Q000737:chemistry*; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000737:chemistry; D000076662:Host Microbial Interactions; D006801:Humans; D055668:Photochemical Processes
keywords: adeno-associated viruses; genetic code expansion; optical regulation; viral entry; virus-host interactions