title: PTPN22 -1123G>C polymorphism and anti-cyclic citrullinated protein antibodies in rheumatoid arthritis.
abstract: OBJECTIVE
  The protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene encodes an important negative regulator of T-cell activation, lymphoid-specific phosphatase -Lyp- and has been associated with different autoimmune disorders. The PTPN22 -1123G>C polymorphism appears to affect the transcriptional control of this gene, but to date, the biological significance of this polymorphisms on rheumatoid arthritis (RA) risk remains unknown. We evaluate the association of PTPN22 -1123G>C polymorphism with anti-cyclic citrullinated protein antibodies (anti-CCP) and risk for RA in population from Western Mexico.
  
  
  METHODS
  A transversal analytic study, which enrolled 300 RA patients classified according to ACR-EULAR criteria and 300 control subjects (CS) was conducted. The -1123 G>C polymorphism was genotyped by PCR-RFLP. The anti-CCP antibodies levels were quantified by ELISA kit.
  
  
  RESULTS
  We found a higher prevalence of homozygous PTPN22 -1123CC genotype in CS than in RA patients (OR 0.41; 95% confidence interval 0.24-0.71; P=.001), suggesting a potential protective effect against RA. Concerning anti-CCP levels, the CC genotype carriers showed the lowest median levels in RA (P<.05).
  
  
  CONCLUSIONS
  The PTPN22 -1123CC genotype is a protector factor to RA in a Mexican-mestizo population and is associated with low anti-CCP antibodies.
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México. Electronic address: biologiamolecular@hotmail.com.
authors.forename: José Francisco
authors.initials: JF
authors.lastname: Muñoz-Valle
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Jorge Ramón
authors.initials: JR
authors.lastname: Padilla-Gutiérrez
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Hernández-Bello
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Yeniley
authors.initials: Y
authors.lastname: Ruiz-Noa
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Yeminia
authors.initials: Y
authors.lastname: Valle
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Claudia Azucena
authors.initials: CA
authors.lastname: Palafox-Sánchez
authors.affiliation: Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México.
authors.forename: Isela
authors.initials: I
authors.lastname: Parra-Rojas
authors.affiliation: Servicio de Reumatología, OPD Hospital Civil de Guadalajara Fray Antonio Alcalde, Guadalajara, Jalisco, México.
authors.forename: Sergio Ramón
authors.initials: SR
authors.lastname: Gutiérrez-Ureña
authors.affiliation: Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Ocotlán, Jalisco, México.
authors.forename: Hector
authors.initials: H
authors.lastname: Rangel-Villalobos
mesh_terms: D000328:Adult; D000075422:Anti-Citrullinated Protein Antibodies / Q000097:blood*; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D060005:Genotyping Techniques; D006720:Homozygote; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D065840:Protective Factors; D054596:Protein Tyrosine Phosphatase, Non-Receptor Type 22 / Q000235:genetics*
chemical_list: D000075422:Anti-Citrullinated Protein Antibodies; D015415:Biomarkers; D005819:Genetic Markers; C516765:PTPN22 protein, human; D054596:Protein Tyrosine Phosphatase, Non-Receptor Type 22
keywords: Anti-cyclic citrullinated protein antibodies; Anticuerpos antipéptido citrulinado cíclico; Artritis reumatoide; Lymphoid-specific phosphatase (Lyp); PTPN22; Proteína linfoide tirosina fosfatasa (Lyp); Rheumatoid arthritis