title: Molecular characterization of an Akabane virus isolate from West Java, Indonesia.
abstract: We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.
authors.affiliation: Disease Investigation Center Subang, Directorate General of Livestock and Animal Health Services, Jl. Terusan Garuda Blok Werasari, RT.33/RW.11, Subang, West Java 41212, Indonesia.
authors.forename: Suryo
authors.initials: S
authors.lastname: Purnomo Edi
authors.forename: Afif
authors.initials: A
authors.lastname: Ibrahim
authors.forename: Rinto
authors.initials: R
authors.lastname: Sukoco
authors.forename: Lukman
authors.initials: L
authors.lastname: Bunali
authors.forename: Masaji
authors.initials: M
authors.lastname: Taguchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kato
authors.forename: Tohru
authors.initials: T
authors.lastname: Yanase
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shirafuji
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle / Q000821:virology*; D002478:Cells, Cultured; D007214:Indonesia; D016851:Orthobunyavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification