title: Techniques for the experimental investigation of the protein corona.
abstract: Due to its enormous relevance the corona formation of adsorbed proteins around nanoparticles is widely investigated. A comparison of different experimental techniques is given. Direct measurements of proteins, such as typically performed with mass spectrometry, will be compared with indirect analysis, in which instead information about the protein corona is gathered from changes in the properties of the nanoparticles. The type of measurement determines also whether before analysis purification from unbound excess proteins is necessary, which may change the equilibrium, or if measurements can be performed in situ without required purification. Pros and contras of the different methods will be discussed.
authors.affiliation: CIC biomaGUNE, San Sebastian, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Carrillo-Carrion
authors.affiliation: CIC biomaGUNE, San Sebastian, Spain; Ikerbasque, Basque Foundation for Science, Bilbao, Spain.
authors.forename: Monica
authors.initials: M
authors.lastname: Carril
authors.affiliation: CIC biomaGUNE, San Sebastian, Spain; Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany; Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, China. Electronic address: wolfgang.parak@physik.uni-marburg.de.
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
mesh_terms: D000327:Adsorption; D001671:Biochemistry / Q000379:methods*; D013058:Mass Spectrometry; D053758:Nanoparticles / Q000737:chemistry; D000066970:Protein Corona / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry
chemical_list: D000066970:Protein Corona; D011506:Proteins