title: Molecular diagnosis of the human immunodeficiency, Hepatitis B and C viruses among blood donors in Lomé (Togo) by multiplex real time PCR.
abstract: This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo. A total of 440 blood samplesfrom volunteer were collected and tested by ELISA and multiplex PCR for HBV, HCV and HIV detection. Among the 440 volunteer blood donors, 83% were female and 17% were male. Age range of 20-29 years was more represented (73%). Whereas, multiplex PCR detected more cases of HBV than ELISA (50% vs 33%, P=0.0155);ELISA more detected HCV than PCR (34% vs 3%, P<0.0001) and HIV (26% vs 7%, P<0.0001). Confirming these observations our data showed that multiplex PCR was more sensitive in the detection of HBV. The sensitivity of ELISA for the detection of HCV and HIV was elevated compared to multiplex PCR. Multiplex PCR was more specific that ELISA for the detection of HCV and HIV.Interestingly, our data showed that the gender do not influenced the sensitivity of either ELISA or multiplex PCR to detect these viruses. This study showed the limit of both ELISA and multiplex PCR in the detection of HBV, HCV and HIV.
authors.affiliation: Center for Biomolecular Research Pietro Annigoni, CERBA/LABIOGENE, University of Ouagadougou, Burkina Faso.
authors.forename: Maléki
authors.initials: M
authors.lastname: Assih
authors.affiliation: National Center for Blood Transfusion (CNTS), Lomé, Togo.
authors.forename: Lochina
authors.initials: L
authors.lastname: Feteke
authors.affiliation: Center for Biomolecular Research Pietro Annigoni, CERBA/LABIOGENE, University of Ouagadougou, Burkina Faso; Laboratory of Molecular and Cellular Biology, University of Sciences and Techniques of Masuku (USTM), Franceville, Gabon.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Bisseye
authors.affiliation: Center for Biomolecular Research Pietro Annigoni, CERBA/LABIOGENE, University of Ouagadougou, Burkina Faso.
authors.forename: Djeneba
authors.initials: D
authors.lastname: Ouermi
authors.affiliation: Center for Biomolecular Research Pietro Annigoni, CERBA/LABIOGENE, University of Ouagadougou, Burkina Faso.
authors.forename: Florencia
authors.initials: F
authors.lastname: Djigma
authors.affiliation: Center for Biomolecular Research Pietro Annigoni, CERBA/LABIOGENE, University of Ouagadougou, Burkina Faso; High School of Food and Biological Techniques (ESTBA-UL), University of Lomé, Togo.
authors.forename: Simplice Damintoti
authors.initials: SD
authors.lastname: Karou
authors.affiliation: Center for Biomolecular Research Pietro Annigoni, CERBA/LABIOGENE, University of Ouagadougou, Burkina Faso.
authors.forename: Jacques
authors.initials: J
authors.lastname: Simpore
mesh_terms: D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006678:HIV / Q000302:isolation & purification; D015658:HIV Infections / Q000175:diagnosis*; D016174:Hepacivirus / Q000302:isolation & purification; D006509:Hepatitis B / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000302:isolation & purification; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014037:Togo; D055815:Young Adult
keywords: HBV; HCV; HIV; Multiplex real time PCR