title: Immunoregulatory effect assessment of a novel melanin and its carboxymethyl derivative.
abstract: A novel melanin with low molecular weight (LIM205, 522Da) was isolated from the fermentation broth of Lachnum sp. and its carboxymethyl derivative (CLIM205) was prepared. The immunoregulatory effects of LIM205 and CLIM205 in immuno-compromised mice induced by cyclophosphamide were investigated. The results demonstrated that both LIM205 and CLIM205 could significantly increase the thymus and spleen indices, specific and nonspecific (including carbon clearance ability) immunity, humoral and cellular immunity of mice. Treatment with LIM205 and CLIM205 could increase activities of SOD, GSH-PX, CAT and decrease content of MDA in the mice. Furthermore, for all animal tests, the immunoregulatory activities of CLIM205 were more prominent than that of LIM205. In conclusion, our findings suggested that the natural products LIM205, as well as its carboxymethyl derivative CLIM205, had significant immunoregulatory activities, which might be a promising source of immunoregulator in healthcare field.
authors.affiliation: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Shi
authors.affiliation: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Jinglei
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Biological and Medical Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Can
authors.initials: C
authors.lastname: Xu
authors.affiliation: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Biological and Medical Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Shaikh
authors.affiliation: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: yeming123@sina.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D007111:Immunity, Cellular / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008543:Melanins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D008543:Melanins
keywords: Carboxymethylated melanin; Cyclophosphamide; Immune regulation effect; Lachnum