title: HLA Genotyping in Patients with End-Stage Renal Disease Waiting For Cadaveric Renal Transplantation in Federation of Bosnia and Herzegovina.
abstract: OBJECTIVE
  The research was conducted by genotyping two Human Leukocyte Antigen (HLA) gene classes. The main objective of this research was to investigate distribution and frequency of the allelic groups, genotypes and haplotypes in the gene loci of HLA class I (HLA-A*, -B*, -C*) and HLA class II (HLA-DRB1*, -DQB1*) in patients included in the program of cadaveric renal transplantation.
  
  
  METHODS
  Our study covered 186 blood samples of patients who are registered on the list for cadaveric renal transplantation in Federation of Bosnia and Herzegovina and included 59 control, healthy unrelated individuals. For the HLA typing, we have used three different methods: micro lymphocyte cytotoxicity test (MLCT), Polymerase Chain Reaction (PCR) - Sequence Specific Primers (SSP) and PCR - Sequence-Specific Oligonucleotides (SSO) or Luminex technology. All patients and cadaveric donors were tested using the three methods because the system is polymorphic.
  
  
  RESULTS
  Analysis of the results of genotyping HLA class I gene loci identified dominant HLA-A*02, HLA-B*35, HLA-C*07 allelic groups. Analysis of the HLA class II gene loci genotyping showed that HLA-DRB1*11 and HLA-DQB1*03 loci had the highest incidence in HLA class II.
  
  
  CONCLUSIONS
  Based on our results and previous research, there were no observed differences between allelic frequencies and genotypes of healthy people and people with ESRD. Differences between allelic groups occurred, but they were not statistically significant, except HLA-C*01 (p = 0.020).
authors.affiliation: Institute of Transfusion Medicine of Federation of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina.
authors.forename: Elma
authors.initials: E
authors.lastname: Fejzić
authors.affiliation: Department of Immunology, Clinical Center University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
authors.forename: Jasenko
authors.initials: J
authors.lastname: Karamehić
authors.affiliation: Department of Biochemistry and Physiology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
authors.forename: Izet
authors.initials: I
authors.lastname: Eminović
authors.affiliation: Department of Biochemistry and Physiology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
authors.forename: Damir
authors.initials: D
authors.lastname: Suljević
authors.affiliation: Department of Biochemistry and Physiology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
authors.forename: Andi
authors.initials: A
authors.lastname: Alijagić
authors.affiliation: Institute of Pathology, Clinical Center University of Tuzla, Tuzla, Bosnia and Herzegovina.
authors.forename: Semir
authors.initials: S
authors.lastname: Bećirević
authors.affiliation: Institute of Transfusion Medicine of Federation of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina.
authors.forename: Amela
authors.initials: A
authors.lastname: Šahović
authors.affiliation: Institute of Transfusion Medicine of Federation of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina.
authors.forename: Sanela
authors.initials: S
authors.lastname: Šišić
keywords: Allele groups; Genotype; Haplotype; Human Leukocyte Antigens (HLA); Major Histocompatibility Complex (MHC)