title: Influence of ultrafiltration membrane on ophiopogonins and homoisoflavonoids in Ophiopogon japonicus as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry.
abstract: Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus (L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off (MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone (PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate (%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China; Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Technology Development, TianJin Tasly Pride Pharmaceutical Co., Ltd., Tianjin, 300410, China.
authors.forename: Da-Zheng
authors.initials: DZ
authors.lastname: Zhou
authors.affiliation: Department of Technology Development, TianJin Tasly Pride Pharmaceutical Co., Ltd., Tianjin, 300410, China. Electronic address: juach@tasly.com.
authors.forename: Ai-Chun
authors.initials: AC
authors.lastname: Ju
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: boyangyu59@163.com.
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Yu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal; D007529:Isoflavones / Q000032:analysis*; D015394:Molecular Structure; D008970:Molecular Weight; D031441:Ophiopogon / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011108:Polymers; D012503:Saponins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013450:Sulfones; D014462:Ultrafiltration / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D010936:Plant Extracts; D011108:Polymers; D012503:Saponins; D013450:Sulfones; C022840:polyether sulfone
keywords: Homoisoflavonoids; Ophiopogon japonicus (L.f) Ker-Gawl.; Ophiopogonins; UFLC-IT-TOF/MS; Ultrafiltration