title: Antibacterial evaluation of Salvia miltiorrhizae on Escherichia coli by microcalorimetry coupled with chemometrics.
abstract: For seeking novel antibacterial agents with high efficacy and low toxicity to deal with drug resistance, the effects of Salvia miltiorrhizae from various sources on Escherichia coli were evaluated by microcalorimetry coupled with chemometrics. Firstly, the heat-flow power-time curves of E. coli growth affected by different S. miltiorrhizae samples were recorded. Then, some crucial quantitative thermo-kinetic parameters including growth rate constant, heat-flow power and heat output, etc. were obtained from theses curves and were further investigated by some powerful chemometric techniques including similarity analysis, multivariate analysis of variance, hierarchical clustering analysis and principle component analysis. By analyzing the principle parameters, growth rate constant of the second exponential phase (k2) and the heat-flow output powers of the second highest peak (P2), together with the derived parameter inhibitory ratio (I,  %), it could be quickly concluded that the tested S. miltiorrhizae samples from different sources in China exhibited strong antibacterial effects on E. coli and the samples from Beijing city exhibited the strongest anti-E. coli effects, which might be used as novel and underlying antibacterial candidates for the resistance of E. coli to the existing drugs in practice. This study provides a useful tool and helpful idea to accurately and rapidly evaluate the antibacterial effects of some complex matrices, offering some references for exploring new antibacterial agents.
authors.affiliation: Pharmacy College, Jinzhou Medical University, Jinzhou, 121001, China.|Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.
authors.forename: Guangyao
authors.initials: G
authors.lastname: Ying
authors.affiliation: Pharmacy College, Jinzhou Medical University, Jinzhou, 121001, China.|Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.|College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
authors.affiliation: Guizhou Xinbang Pharmaceutical Co. Ltd., Guiyang, 550014, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Guizhou Xinbang Pharmaceutical Co. Ltd., Guiyang, 550014, People's Republic of China.
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Wu
authors.affiliation: Pharmacy College, Jinzhou Medical University, Jinzhou, 121001, China. wyinguo@163.com.
authors.forename: Weiying
authors.initials: W
authors.lastname: Guo
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China. kongwj302@126.com.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
keywords: Antibacterial evaluation; Chemometrics; Escherichia coli; Microcalorimetry; Salvia miltiorrhizae