title: Effects of Low-Protein Diets Supplemented with Branched-Chain Amino Acid on Lipid Metabolism in White Adipose Tissue of Piglets.
abstract: This study evaluated the effect of branched-chain amino acid (BCAA) supplementation in low-protein diets on lipid metabolism in dorsal subcutaneous adipose (DSA), abdominal subcutaneous adipose (ASA), and perirenal adipose (PRA) tissues. A total of 24 piglets were allotted to four treatments, and each group was fed the adequate protein (AP) diet, low-protein (LP) diet, LP diet supplemented with BCAA (LP + B), or LP diet supplemented with twice BCAA (LP + 2B). Serum concentrations of leptin in the BCAA-supplemented treatments were higher (P < 0.01) than in the AP treatment, but lower (P < 0.01) than in the LP treatment. In DSA, the mRNA and protein levels for lipogenic-related genes were highest in the LP treatment and lowest in the LP + 2B treatment. However, in ASA and PRA, the expression levels for those genes were significantly elevated in the LP + 2B treatment. In conclusion, BCAA supplementation could alter the body fat condition, and this effect was likely modulated by the expression of lipid metabolic regulators in DSA, ASA, and PRA in a depot-specific manner.
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, China.|University of Chinese Academy of Sciences , Beijing 100039, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Sciences, Huazhong Agricultural University , Wuhan, Hubei 430070, China.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Wei
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, China.|Hunan Co-Innovation Center of Animal Production Safety, CICAPS; Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients , Changsha, Hunan 410128, China.
authors.forename: Fengna
authors.identifier: http://orcid.org/0000-0002-0040-9784
authors.initials: F
authors.lastname: Li
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, China.|University of Chinese Academy of Sciences , Beijing 100039, China.
authors.forename: Yehui
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, China.|University of Chinese Academy of Sciences , Beijing 100039, China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, China.|Laboratory of Animal Nutrition and Human Health, School of Biology, Hunan Normal University , Changsha, Hunan 410018, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism*; D000597:Amino Acids, Branched-Chain / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D018753:Diet, Protein-Restricted; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D020738:Leptin; D050356:Lipid Metabolism; D050155:Lipogenesis; D008297:Male; D013552:Swine / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000597:Amino Acids, Branched-Chain; D020738:Leptin
keywords: branched-chain amino acids; lipid metabolism; low-protein diet; piglet; white adipose tissue