title: Bovine peripheral blood WC1+and WC1negγδ T lymphocytes modulate monocyte-derived macrophage effector functions during in vitro Mycobacterium avium subspecies paratuberculosis infection.
abstract: The importance of bovine γδ T lymphocytes during anti-mycobacterial immunity is recognized; however, the role of major subsets of γδ T lymphocytes (WC1+and WC1neg) in this process remains unclear. We investigated how WC1+and WC1negγδ T lymphocyte subsets of calves modulate monocyte-derived macrophage (MDM) functions during Map infection in vitro. To achieve this, Map-infected or uninfected MDMs from young calves were co-cultured with autologous WC1+or WC1negγδ T lymphocytes. Our data indicate that WC1+and WC1negγδ T lymphocytes of young calves modulate effector functions of MDMs with respect to Map killing, CD11b and MHC-II expression. We observed differences in IFN-γ production and CD25 expression on γδ T lymphocyte subsets, as well as MDM expression of CD1b when in contact with WC1negγδ T lymphocytes.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Pathobiology/AHL Building 89, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada. Electronic address: mbaquero@uguelph.ca.
authors.forename: Monica M
authors.initials: MM
authors.lastname: Baquero
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Pathobiology/AHL Building 89, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Brandon L
authors.initials: BL
authors.lastname: Plattner
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D058491:Bacterial Load; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis / Q000494:pharmacology; D018655:Lymphocyte Count; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis; D009000:Monocytes / Q000276:immunology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000254:growth & development / Q000276:immunology; D010283:Paratuberculosis / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C086500:DMBT1 protein, Bos taurus; D008562:Membrane Glycoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma
keywords: Ages; Cattle immunology; Macrophages; Mycobacterium avium subspecies paratuberculosis; WC1; γδ T lymphocytes