title: FADD, Caspase-3, and Caspase-8 and Incidence of Coronary Events.
abstract: To investigate the relationship between 3 markers of apoptosis, that is, FADD (Fas-associated death domain-containing protein), caspase-3, and caspase-8, and incidence of coronary events (CEs) in a population-based cohort study.
  
  
  
  In vitro experiments were performed to assess the response of the apoptotic biomarkers after Fas stimulation of peripheral blood mononuclear cells. The experiments showed significantly increased releases of FADD, caspase-3, and caspase-8 after Fas stimulation. The relationship between FADD, caspase-3, and caspase-8, respectively, and incidence of CEs was studied in 4284 subjects from the population-based Malmö Diet and Cancer Study. Cox' proportional hazards regression was used to examine the association between the apoptotic biomarkers and incidence of CE over a mean follow-up of 19 years. A total of 381 individuals had CE during the follow-up. High FADD at baseline was significantly associated with incident CE. In the highest compared with the lowest quartile of FADD, the risk factor adjusted hazards ratio for CE was 1.82 (95% confidence interval, 1.35-2.46;Pfor trend <0.001). A significant association was also found between caspase-8 and CE; the hazards ratio (Q4 versus Q1) was 1.90 (95% confidence interval, 1.39-2.60;Pfor trend <0.001) after adjustment for risk factors. No association was found between caspase-3 and CEs.
  
  
  
  High levels of FADD and caspase-8, but not caspase-3, were associated with increased incidence of CE in subjects from the general population. The in vitro experiments support the view that these biomarkers could reflect activation of the extrinsic apoptotic pathway.
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.). xueling112001@163.com Gunnar.Engstrom@med.lu.se.
authors.forename: Ling
authors.initials: L
authors.lastname: Xue
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Yan
authors.initials: Y
authors.lastname: Borné
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Ingrid Yao
authors.initials: IY
authors.lastname: Mattisson
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Maria
authors.initials: M
authors.lastname: Wigren
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Olle
authors.initials: O
authors.lastname: Melander
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Marju
authors.initials: M
authors.lastname: Ohro-Melander
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Eva
authors.initials: E
authors.lastname: Bengtsson
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Gunilla Nordin
authors.initials: GN
authors.lastname: Fredrikson
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.).
authors.forename: Jan
authors.initials: J
authors.lastname: Nilsson
authors.affiliation: From the Department of Cardiovascular Diseases, the Second Hospital of Hebei Medical University, ShiJiaZhuang, China (L.X.); and Department of Clinical Sciences, Malmö, Lund University, Sweden (Y.B., I.Y.M., M.W., O.M., M.O.-M., E.B., G.N.F., J.N., G.E.). xueling112001@163.com Gunnar.Engstrom@med.lu.se.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Engström
mesh_terms: D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D053148:Caspase 3 / Q000097:blood*; D053181:Caspase 8 / Q000097:blood*; D002478:Cells, Cultured; D003327:Coronary Disease / Q000097:blood* / Q000000981:diagnostic imaging / Q000453:epidemiology* / Q000473:pathology; D053200:Fas-Associated Death Domain Protein / Q000097:blood*; D005260:Female; D006801:Humans; D015994:Incidence; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D012307:Risk Factors; D013548:Sweden / Q000453:epidemiology; D013997:Time Factors; D015854:Up-Regulation; D019014:fas Receptor / Q000494:pharmacology
chemical_list: D015415:Biomarkers; C505533:FADD protein, human; D053200:Fas-Associated Death Domain Protein; D019014:fas Receptor; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8
keywords: FADD; caspase-3; caspase-8; coronary events