title: Genomic confirmation of vancomycin-resistant Enterococcus transmission from deceased donor to liver transplant recipient.
abstract: In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing identified that donor and recipient VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison of de novo assembled isolate genomes was highly suggestive of transplant transmission rather than hospital-acquired transmission and also identified subtle internal rearrangements between donor and recipient missed by other genomic approaches. Given the improved resolution, whole-genome assembly of pathogen genomes is likely to become an essential tool for investigation of potential organ transplant transmissions.
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Ali
authors.identifier: http://orcid.org/0000-0002-5240-9604
authors.initials: A
authors.lastname: Bashir
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Oliver
authors.initials: O
authors.lastname: Attie
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Mitchell
authors.initials: M
authors.lastname: Sullivan
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Robert
authors.initials: R
authors.lastname: Sebra
authors.affiliation: Division of Infectious Diseases, Department of Medicine, The University of Texas Medical School, Houston, TX.
authors.forename: Kavindra V
authors.initials: KV
authors.lastname: Singh
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Deena
authors.initials: D
authors.lastname: Altman
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Theodore
authors.initials: T
authors.lastname: Pak
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Jayeeta
authors.initials: J
authors.lastname: Dutta
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Kieran
authors.initials: K
authors.lastname: Chacko
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Webster
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Martha
authors.initials: M
authors.lastname: Lewis
authors.affiliation: Department of Pathology, Icahn School of Medicine, New York, NY.
authors.forename: Camille
authors.initials: C
authors.lastname: Hamula
authors.affiliation: LiveOnNY, New York, NY.
authors.forename: Kristin W
authors.initials: KW
authors.lastname: Delli Carpini
authors.affiliation: Division of Infectious Diseases, Department of Medicine, The University of Texas Medical School, Houston, TX.
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Murray
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Andrew
authors.initials: A
authors.lastname: Kasarskis
authors.affiliation: Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Harm
authors.initials: H
authors.lastname: van Bakel
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
authors.forename: Shirish
authors.initials: S
authors.lastname: Huprikar
mesh_terms: D000368:Aged; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D014019:Tissue Donors; D065507:Vancomycin-Resistant Enterococci / Q000235:genetics*