title: Loblolly pine grown under elevated CO2affects early instar pine sawfly performance.
abstract: Seedlings of loblolly pine Pinus taeda (L.), were grown in open-topped field chambers under three CO2regimes: ambient, 150 μl l-1CO2above ambient, and 300 μl l-1CO2above ambient. A fourth, non-chambered ambient treatment was included to assess chamber effects. Needles were used in 96 h feeding trials to determine the performance of young, second instar larvae of loblolly pine's principal leaf herbivore, red-headed pine sawfly, Neodiprion lecontei (Fitch). The relative consumption rate of larvae significantly increased on plants grown under elevated CO2, and needles grown in the highest CO2regime were consumed 21% more rapidly than needles grown in ambient CO2. Both the significant decline in leaf nitrogen content and the substantial increase in leaf starch content contributed to a significant increase in the starch:nitrogen ratio in plants grown in elevated CO2. Insect consumption rate was negatively related to leaf nitrogen content and positively related to the starch:nitrogen ratio. Of the four volatile leaf monoterpenes measured, only β-pinene exhibited a significant CO2effect and declined in plants grown in elevated CO2. Although consumption changed, the relative growth rates of larvae were not different among CO2treatments. Despite lower nitrogen consumption rates by larvae feeding on the plants grown in elevated CO2, nitrogen accumulation rates were the same for all treatments due to a significant increase in nitrogen utilization efficiency. The ability of this insect to respond at an early, potentially susceptible larval stage to poorer food quality and declining levels of a leaf monoterpene suggest that changes in needle quality within pines in future elevated-CO2atmospheres may not especially affect young insects and that tree-feeding sawflies may respond in a manner similar to herb-feeding lepidopterans.
authors.affiliation: Department of Biological Sciences, University of South Carolina, 29208, Columbia, SC, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Williams
authors.affiliation: Department of Biological Sciences, University of South Carolina, 29208, Columbia, SC, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Lincoln
authors.affiliation: Botany Department, Duke University, 27706, Durham, NC, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Thomas
keywords: Elevated CO2; Neodiprion lecontei; Nitrogen utilization; Pinus taeda; Plant-insect interaction