title: Dynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquifer.
abstract: To further understand the diversity and dynamics of SRB in response to substrate amendment, we sequenced genes coding for the dissimilatory sulfite reductase (dsrA) in groundwater samples collected after an emulsified vegetable oil (EVO) amendment, which sustained U(VI)-reducing conditions for one year in a fast-flowing aquifer. EVO amendment significantly altered the composition of groundwater SRB communities. Sequences having no closely related-described species dominated (80%) the indigenous SRB communities in nonamended wells. After EVO amendment, Desulfococcus, Desulfobacterium, and Desulfovibrio, known for long-chain-fatty-acid, short-chain-fatty-acid and H2oxidation and U(VI) reduction, became dominant accounting for 7 ± 2%, 21 ± 8%, and 55 ± 8% of the SRB communities, respectively. Succession of these SRB at different bioactivity stages based on redox substrates/products (acetate, SO4-2, U(VI), NO3-, Fe(II), and Mn(II)) was observed. Desulfovibrio and Desulfococcus dominated SRB communities at 4-31 days, whereas Desulfobacterium became dominant at 80-140 days. By the end of the experiment (day 269), the abundance of these SRB decreased but the overall diversity of groundwater SRB was still higher than non-EVO controls. Up to 62% of the SRB community changes could be explained by groundwater geochemical variables, including those redox substrates/products. A significant (P < 0.001) correlation was observed between groundwater U(VI) concentrations and Desulfovibrio abundance. Our results showed that the members of SRB and their dynamics were correlated significantly with slow EVO biodegradation, electron donor production and maintenance of U(VI)-reducing conditions in the aquifer.
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Ping
authors.identifier: 0000-0002-9455-2265
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Zhili
authors.initials: Z
authors.lastname: He
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Joy D
authors.initials: JD
authors.lastname: Van Nostrand
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.|Research Center for Eco-Environmental Science, Chinese Academy of Sciences , Beijing 100085, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Liyou
authors.initials: L
authors.lastname: Wu
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.|Department of Marine Sciences, Ocean College, Zhejiang University , Zhejiang, China.
authors.forename: Qichao
authors.initials: Q
authors.lastname: Tu
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.|State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences , Nanjing 210008, China.
authors.forename: Jianjun
authors.identifier: 0000-0001-7039-7136
authors.initials: J
authors.lastname: Wang
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Schadt
authors.affiliation: Center for Biofilm Engineering, Montana State University , Bozeman, Montana 59717, United States.
authors.forename: Matthew
authors.identifier: 0000-0001-9053-1849
authors.initials: M
authors.lastname: W Fields
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Civil and Environmental Engineering, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors.forename: Terry C
authors.initials: TC
authors.lastname: Hazen
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Arkin
authors.affiliation: Department of Civil and Environmental Engineering, University of Washington , Seattle, Washington 98105, United States.
authors.forename: David A
authors.initials: DA
authors.lastname: Stahl
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, and School of Civil Engineering and Environmental Sciences, University of Oklahoma , Norman, Oklahoma 73019, United States.|Earth and Environmental Sciences, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University , Beijing 100084, China.
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D060587:Groundwater / Q000737:chemistry*; D010084:Oxidation-Reduction; D013431:Sulfates / Q000737:chemistry; D013461:Sulfur Oxides; D014501:Uranium / Q000378:metabolism*
chemical_list: D013431:Sulfates; D013461:Sulfur Oxides; D014501:Uranium