title: Exploiting oleuropein for inhibiting collagen fibril formation.
abstract: Collagen fibrils accumulate in excessive amounts and impair the normal functioning of the organ; therefore it stimulates the interest for identifying the compounds that could prevent the formation of fibrils. Herein, inhibition of self-assembly of collagen using oleuropein has been studied. The changes in the physico-chemical characteristics of collagen on interaction with increasing concentration of oleuropein has been studied using techniques like viscosity, UV-vis, CD and FT-IR. The inhibitory effect of oleuropein on fibril formation of collagen was proved using SEM. Circular dichroism and FT-IR spectra elucidates the alterations in the secondary structure of collagen suggesting non-covalent interactions between oleuropein and collagen. The decreased rate of collagen fibril formation also confirms the inhibition in the self-assembly of collagen. Hence, our study suggests that inhibition of the self-assembly process using oleuropein may unfold new avenues to treat fibrotic diseases.
authors.affiliation: Inorganic and Physical Chemistry Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, India.
authors.forename: H
authors.initials: H
authors.lastname: Bharathy
authors.affiliation: Inorganic and Physical Chemistry Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, India. Electronic address: nishad@clri.res.in.
authors.forename: N Nishad
authors.initials: NN
authors.lastname: Fathima
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000494:pharmacology*; D066329:Protein Aggregates / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D051381:Rats
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; D066329:Protein Aggregates; C002769:oleuropein; D003094:Collagen
keywords: Collagen fibril formation; FT-IR; Oleuropein; SEM; Self-assembly