title: Ectopic expression of Arabidopsis FD and FD PARALOGUE in rice results in dwarfism with size reduction of spikelets.
abstract: Key flowering genes, FD and FD PARALOGUE (FDP) encoding bZIP transcription factors that interact with a FLOWERING LOCUS T (FT) in Arabidopsis were ectopically expressed in rice since we found AtFD and AtFDP also interact with HEADING DATE 3a (Hd3a) and RICE FLOWERING LOCUS T 1 (RFT1). Transgenic rice plants overexpressing AtFD and AtFDP caused reduction in plant height and spikelet size with decreased expression of genes involved in cell elongation without significant flowering time alteration in spite of increased expression of OsMADS14 and OsMADS15, rice homologues of APETALA1 (AP1) in the leaves. Simultaneous overexpression of AtFD and AtFDP enhanced phenotypes seen with overexpression of either single gene while transgenic rice plants expressing AtFD or AtFDP under the control of phloem-specific Hd3a promoter were indistinguishable from wild-type rice. Candidate genes responsible for the phenotypes were identified by comparison of microarray hybridization and their expression pattern was also examined in WT and transgenic rice plants. It has so far not been reported that AtFD and AtFDP affect cell elongation in plants, and our findings provide novel insight into the possible roles of AtFD and AtFDP in the mesophyll cells of plants, and potential genetic tools for manipulation of crop architecture.
authors.affiliation: Biotechnology Center in Southern Taiwan (BCST), No. 59 Siraya Blvd., Xinshi Dist., Tainan 74145/Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 11529, Taiwan.|Institute of Tropical Plant Science, National Cheng Kung University, No. 1 University Road, East Dist., Tainan 70101, Taiwan.
authors.forename: Seonghoe
authors.initials: S
authors.lastname: Jang
authors.affiliation: Biotechnology Center in Southern Taiwan (BCST), No. 59 Siraya Blvd., Xinshi Dist., Tainan 74145/Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 11529, Taiwan.
authors.forename: Hsing-Yi
authors.initials: HY
authors.lastname: Li
authors.affiliation: Biotechnology Center in Southern Taiwan (BCST), No. 59 Siraya Blvd., Xinshi Dist., Tainan 74145/Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 11529, Taiwan.
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Kuo
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D000066630:Ectopic Gene Expression / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D017440:Photoperiod; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; C503117:FD protein, Arabidopsis; D014157:Transcription Factors