title: Neural control of RNA synthesis in regenerating limbs of the adult newtTriturus viridescens.
abstract: Polyacrylamide gel electrophoresis was used to investigate the role of nerves in controlling patterns of RNA synthesis in regenerating limbs of the adult newt,Triturus viridescens. Denervation has the same effect on nerve-dependent and independent stages of regeneration, reducing by approximately 40-50% the synthesis of ribosomal and transfer RNA. Although a differential qualitative response of messenger RNA synthesis to denervation between nerve-dependent and independent stages has not been ruled out, the results would indicate that the effect of the nerve on RNA metabolism in individual blastema cells is the same over the whole process of regeneration. Since the one constant effect of denervation on regeneration is to inhibit regenerate growth in volume, the emancipation of blastemal morphogenetic activity from nerve requirements is more likely to be a function of attaining a critical mass of blastema cells, rather than a change in the metabolic response of blastema cells to the nerve.
authors.affiliation: Department of Zoology, University of Illinois, 61801, Urbana, Illinois.
authors.forename: Frank V
authors.initials: FV
authors.lastname: Morzlock
authors.affiliation: Department of Zoology, University of Illinois, 61801, Urbana, Illinois.
authors.forename: David L
authors.initials: DL
authors.lastname: Stocum