title: In vitro development of theDrosophila chorion in a chemically defined organ culture medium.
abstract: TheDrosophila chorion is produced normally in isolated follicles in Robb's chemically defined culture medium. The complex architecture of the shell developed in vitro from follicles as young as early stage 10 is completely normal morphologically. In addition, the time required for in vitro development closely approximates that observed for in vivo development. Comparisons of insect culture media developed by Robb, Grace, Schneider, and Echalier show large variations in their ability to supportDrosophila chorion development.
authors.affiliation: Biology Department, Boston College, 02167, Chestnut Hill, Massachusetts, USA.
authors.forename: William H
authors.initials: WH
authors.lastname: Petri
authors.affiliation: Biology Department, Boston College, 02167, Chestnut Hill, Massachusetts, USA.
authors.forename: Mihalis N
authors.initials: MN
authors.lastname: Mindrinos
authors.affiliation: Biology Department, Boston College, 02167, Chestnut Hill, Massachusetts, USA.
authors.forename: Mary F
authors.initials: MF
authors.lastname: Lombard
authors.affiliation: Department of Biology, University of Athens, Panepistemiopolis, Kouponia, (621), Athens, Greece.
authors.forename: Lukas H
authors.initials: LH
authors.lastname: Margaritis
keywords: Chorion; Drosophila; Eggshell; In vitro development; Tissue culture media