title: SPARC synthesis in pre-implantation and early post-implantation mouse embryos.
abstract: SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin and BM-40, is a secreted protein associated with a variety of embryonic and adult tissue and cell types, including placenta, parietal and visceral endoderm, certain epithelia (e.g. gut, skin, glandular epithelia), and regions of active chondrogenesis and osteogenesis. Although much is known concerning the tissue distribution of this protein, neither the time and location of its initial appearance nor its functions during embryogenesis have been clearly established. We identified the location of SPARC on two-dimensional protein gels. By using two-dimensional gel analysis of both pre- and post-implantation stage mouse embryos, we find that SPARC is initially synthesized between 3.5 and 4.5 days of embryogenesis. This is the earliest time during development at which synthesis of SPARC has been demonstrated. Inner cell masses isolated from 4.5 day blastocysts synthesize SPARC indicating that either primitive ectoderm, primitive endoderm, or both produce this protein. SPARC synthesis is also detectable in isolated trophoblast vesicles. Thus, SPARC is synthesized not only in placenta, parietal endoderm, and visceral endoderm, but in the precursors of these tissues as well. Examination of 7.5 day embryos reveals that SPARC is synthesized in isolated parietal yolk sac and in whole extraembryonic and embryonic regions. Relative to other proteins, synthesis of SPARC was most prevalent in the parietal yolk sac. The possible implications of SPARC synthesis as early as 4.5 days are discussed.
authors.affiliation: The Wistar Institute of Anatomy and Biology, 36th and Spruce Streets, 19104, Philadelphia, PA, USA.
authors.forename: Keith E
authors.initials: KE
authors.lastname: Latham
authors.affiliation: The Wistar Institute of Anatomy and Biology, 36th and Spruce Streets, 19104, Philadelphia, PA, USA.
authors.forename: Chin C
authors.initials: CC
authors.lastname: Howe
keywords: Mouse embryo; Osteonectin; SPARC; Two-dimensional gel electrophoresis