title: DCEBIO facilitates myogenic differentiation via intermediate conductance Ca2+activated K+channel activation in C2C12 myoblasts.
abstract: Membrane hyperpolarization is suggested to be a trigger for skeletal muscle differentiation. We investigated whether DCEBIO, an opener of the small/intermediate conductance Ca2+activated K+(SKCa/IKCa) channels, increase myogenic differentiation in C2C12 skeletal myoblasts. DCEBIO significantly increased myotube formation, protein expression level of myosin heavy chain II, and mRNA expression level of myogenin in C2C12 myoblasts cultured in differentiation medium. DCEBIO induced myotube formation and hyperpolarization were reduced by the IKCachannel blocker TRAM-34, but not by the SKCachannel blocker apamin. These findings show that DCEBIO increases myogenic differentiation by activating IKCachannels.
authors.affiliation: Department of Pharmacology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, Fukushima, 960-1295, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Department of Pharmacology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, Fukushima, 960-1295, Japan; Emergency and Critical Care Medical Center, Fukushima Medical University Hospital, 1 Hikarigaoka, Fukushima, Fukushima, 960-1295, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ono
authors.affiliation: Department of Pharmacology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, Fukushima, 960-1295, Japan. Electronic address: kazuho@fmu.ac.jp.
authors.forename: Kazuho
authors.initials: K
authors.lastname: Sakamoto
mesh_terms: D001030:Apamin / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018485:Muscle Fibers, Skeletal / Q000187:drug effects; D018482:Muscle, Skeletal / Q000166:cytology; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D024681:Potassium Channels, Calcium-Activated / Q000037:antagonists & inhibitors / Q000187:drug effects*; D011720:Pyrazoles / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C507351:5,6-dichloro-1-ethyl-1,3-dihydro-2H-benzimidazol-2-one; D001562:Benzimidazoles; D018008:Myogenin; D024681:Potassium Channels, Calcium-Activated; D011720:Pyrazoles; D012333:RNA, Messenger; C411671:TRAM 34; D001030:Apamin; D018995:Myosin Heavy Chains
keywords: Ca(2+) activated K(+) channel; Myogenic differentiation; Skeletal muscle