title: Embryonic expression of the engrailed homologue of Rhynchosciara americana.
abstract: The segment polarity gene engrailed is involved in the determination of segment posterior identity in Drosophila. engrailed has been largely used for comparative developmental studies due to its evolutionary conservation from nematodes to humans. By in situ hybridization of an engrailed cDNA probe from Drosophila to polytene chromosomes of fourth instar larvae of Rhynchosciara americana we have shown that engrailed-like sequences must be localized in band 6 of chromosome A in this species. The pattern of engrailed protein expression during R. americana embryo development is diffuse at first evolving into a nuclear striped pattern after quite a length of time. In addition, our results suggest a possible developmentally regulated molecular modification of engrailed protein in R. americana embryos.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Laboratorio de Biologia Molecular Maury Miranda, CCS, Bloco G, S-044, Cidade Universitaria, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: C E
authors.initials: CE
authors.lastname: Vanario-Alonso
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Laboratorio de Biologia Molecular Maury Miranda, CCS, Bloco G, S-044, Cidade Universitaria, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Sutton
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Laboratorio de Biologia Molecular Maury Miranda, CCS, Bloco G, S-044, Cidade Universitaria, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Carvalho
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Laboratorio de Biologia Molecular Maury Miranda, CCS, Bloco G, S-044, Cidade Universitaria, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Yussa
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Laboratorio de Biologia Molecular Maury Miranda, CCS, Bloco G, S-044, Cidade Universitaria, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: T M C
authors.initials: TM
authors.lastname: Silva
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Laboratorio de Biologia Molecular Maury Miranda, CCS, Bloco G, S-044, Cidade Universitaria, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Abdelhay
keywords: Embryonic development; Gene expression; Rhynchosciara americana; Segmentation; engrailed