title: Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters.
abstract: The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.
authors.affiliation: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.|Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Hussam Hassan
authors.identifier: 0000-0001-6660-0509
authors.initials: HH
authors.lastname: Nour-Eldin
authors.affiliation: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.|Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Svend Roesen
authors.initials: SR
authors.lastname: Madsen
authors.affiliation: Bayer CropScience NV, Innovation Center, Zwijnaarde, Belgium.
authors.forename: Steven
authors.initials: S
authors.lastname: Engelen
authors.affiliation: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.|Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Morten Egevang
authors.identifier: 0000-0001-6503-0495
authors.initials: ME
authors.lastname: Jørgensen
authors.affiliation: Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Carl Erik
authors.initials: CE
authors.lastname: Olsen
authors.affiliation: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.|Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Jonathan Sonne
authors.initials: JS
authors.lastname: Andersen
authors.affiliation: Bayer CropScience NV, Innovation Center, Zwijnaarde, Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Seynnaeve
authors.affiliation: Bayer CropScience NV, Innovation Center, Zwijnaarde, Belgium.
authors.forename: Thalia
authors.initials: T
authors.lastname: Verhoye
authors.affiliation: Bayer CropScience Inc., Seed Quality Lab, Saskatoon, Saskatchewan, Canada.
authors.forename: Rudy
authors.initials: R
authors.lastname: Fulawka
authors.affiliation: Bayer CropScience NV, Innovation Center, Zwijnaarde, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Denolf
authors.affiliation: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.|Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Barbara Ann
authors.initials: BA
authors.lastname: Halkier
mesh_terms: D001937:Brassica / Q000235:genetics*; D024861:Genetic Enhancement / Q000379:methods*; D005961:Glucosinolates / Q000032:analysis / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009154:Mutation; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000235:genetics*
chemical_list: D005961:Glucosinolates; D009004:Monosaccharide Transport Proteins; D010938:Plant Oils