title: Comparison of proteomic profiles in the zebrafish retina during experimental degeneration and regeneration.
abstract: Zebrafish spontaneously regenerate the retina after injury. Although the gene expression profile has been extensively studied in this species during regeneration, this does not reflect protein function. To further understand the regenerative process in the zebrafish, we compared the proteomic profile of the retina during injury and upon regeneration. Using two-dimensional difference gel electrophoresis (2D-DIGE) and label-free quantitative proteomics (quadrupole time of flight LC-MS/MS), we analysed the retina of adult longfin wildtype zebrafish at 0, 3 and 18 days after Ouabain injection. Gene ontology analysis indicates reduced metabolic processing, and increase in fibrin clot formation, with significant upregulation of fibrinogen gamma polypeptide, apolipoproteins A-Ib and A-II, galectin-1, and vitellogenin-6 during degeneration when compared to normal retina. In addition, cytoskeleton and membrane transport proteins were considerably altered during regeneration, with the highest fold upregulation observed for tubulin beta 2 A, histone H2B and brain type fatty acid binding protein. Key proteins identified in this study may play an important role in the regeneration of the zebrafish retina and investigations on the potential regulation of these proteins may lead to the design of protocols to promote endogenous regeneration of the mammalian retina following retinal degenerative disease.
authors.affiliation: National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 9EL, United Kingdom.
authors.forename: Karen
authors.initials: K
authors.lastname: Eastlake
authors.affiliation: Centre for Translational Omics, UCL Great Ormond Street Institute of Child Health, 30 Guilford St, London WC1N 1EH, United Kingdom.
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Heywood
authors.affiliation: National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 9EL, United Kingdom.
authors.forename: Dhani
authors.initials: D
authors.lastname: Tracey-White
authors.affiliation: National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 9EL, United Kingdom.
authors.forename: Erika
authors.initials: E
authors.lastname: Aquino
authors.affiliation: Centre for Translational Omics, UCL Great Ormond Street Institute of Child Health, 30 Guilford St, London WC1N 1EH, United Kingdom.
authors.forename: Emily
authors.initials: E
authors.lastname: Bliss
authors.affiliation: Department of Microbiology and Immunology, University of Maryland School of Medicine and IMET, Columbus Center, 701 E, Pratt Street, 3061/3062, Baltimore, USA.
authors.forename: Gerardo R
authors.initials: GR
authors.lastname: Vasta
authors.affiliation: Centre for Translational Omics, UCL Great Ormond Street Institute of Child Health, 30 Guilford St, London WC1N 1EH, United Kingdom.
authors.forename: Kevin
authors.initials: K
authors.lastname: Mills
authors.affiliation: National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 9EL, United Kingdom.
authors.forename: Peng T
authors.initials: PT
authors.lastname: Khaw
authors.affiliation: National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 9EL, United Kingdom.
authors.forename: Mariya
authors.initials: M
authors.lastname: Moosajee
authors.affiliation: National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 9EL, United Kingdom.
authors.forename: G Astrid
authors.initials: GA
authors.lastname: Limb
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D063990:Gene Ontology; D006657:Histones / Q000378:metabolism; D007267:Injections; D010042:Ouabain / Q000008:administration & dosage / Q000494:pharmacology; D040901:Proteomics / Q000379:methods*; D012038:Regeneration / Q000187:drug effects; D015203:Reproducibility of Results; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012162:Retinal Degeneration / Q000378:metabolism* / Q000473:pathology; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D001053:Apolipoproteins; D003598:Cytoskeletal Proteins; D005136:Eye Proteins; D006657:Histones; D029961:Zebrafish Proteins; D010042:Ouabain; D005337:Fibrin