title: De NovoHuman Cardiac Myocytes for Medical Research: Promises and Challenges.
abstract: The advent of cellular reprogramming technology has revolutionized biomedical research.De novohuman cardiac myocytes can now be obtained from direct reprogramming of somatic cells (such as fibroblasts), from induced pluripotent stem cells (iPSCs, which are reprogrammed from somatic cells), and from human embryonic stem cells (hESCs). Suchde novohuman cardiac myocytes hold great promise forin vitrodisease modeling and drug screening andin vivocell therapy of heart disease. Here, we review the technique advancements for generatingde novohuman cardiac myocytes. We also discuss several challenges for the use of such cells in research and regenerative medicine, such as the immature phenotype and heterogeneity ofde novocardiac myocytes obtained with existing protocols. We focus on the recent advancements in addressing such challenges.
authors.affiliation: University of Ottawa Heart Institute & Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Veronique
authors.initials: V
authors.lastname: Hamel
authors.affiliation: Department of Cardiology, Xi'an No. 3 Hospital, Xi'an, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, Xi'an Central Hospital, Xi'an, China.
authors.forename: Shudan
authors.initials: S
authors.lastname: Liao
authors.affiliation: University of Ottawa Heart Institute & Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada; Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Aizhu
authors.initials: A
authors.lastname: Lu
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: University of Ottawa Heart Institute & Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Yucai
authors.initials: Y
authors.lastname: Xie
authors.affiliation: University of Ottawa Heart Institute & Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Wenbin
authors.identifier: 0000-0002-7272-9920
authors.initials: W
authors.lastname: Liang