title: Prevalence of Taxa of Pasteurellaceae Among Populations of Healthy Captive Psittacine Birds.
abstract: Sixty-two strains of Pasteurellaceae-like bacteria were isolated from the tracheas of 87 clinically healthy psittacine birds in two Danish zoos. The isolates were identified by a combination of rpoB and 16S rRNA gene sequencing and by matrix-assisted laser desorption-ionization time of flight. Twenty-eight strains belonged to the genus Volucribacter or were related to this genus and to the unnamed taxon 34 of Bisgaard, and 28 strains were related to the unnamed taxon 44 of Bisgaard. Four strains were identified as Pasteurella multocida , two isolates were classified with the related taxon 45 of Bisgaard, and a single isolate was classified as Pasteurella sp. The investigation documented an unrecognized reservoir of rarely reported and unclassified or unnamed species of Pasteurellaceae-like bacteria in psittacine birds. The results were in accordance with a recent report on isolation of Pasteurellaceae from diseased psittacine birds, and the investigation documented that the same taxa of Pasteurellaceae-like bacteria can be isolated from apparently healthy birds as well as from diseased birds.
authors.affiliation: A Bisgaard Consulting, Viby, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Bisgaard
authors.affiliation: B School of Life Science, Beijing Institute of Technology, Beijing 100081, People's Republic of China.|C Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
authors.forename: Di
authors.initials: D
authors.lastname: Xin
authors.affiliation: C Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.|D Center for Zoo and Wild Animal Health, Copenhagen Zoo, Roskildevej 38, DK-2000 Frederiksberg, Denmark.
authors.forename: M F
authors.initials: MF
authors.lastname: Bertelsen
authors.affiliation: C Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
authors.forename: A M
authors.initials: AM
authors.lastname: Bojesen
authors.affiliation: C Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Christensen
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D004269:DNA, Bacterial / Q000235:genetics; D015661:Pasteurellaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016871:Pasteurellaceae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D015995:Prevalence; D011563:Psittaciformes / Q000821:virology*
chemical_list: D004269:DNA, Bacterial
keywords: MALDI-TOF; Volucribacter psittacicida; rpoB; taxon 44 of Bisgaard