title: Temporally and spatially restricted expression of a gland cell gene during regeneration and in vitro transdifferentiation in the hydrozoan Podocoryne carnea.
abstract: An antiserum to transdifferentiated striated muscle cells from the medusa of Podocoryne carnea was prepared and used to screen a λ gt11-expression library prepared from gonozoids of P. carnea. We isolated a cDNA clone termed Pod-EPPT with at least 63 tandem repeats of the tetrapeptide-motive glu-pro-pro-thr, named Pod-EPPT. Using Pod-EPPT as a molecular marker for head quality the morphological relationship between the two metagenic life stages of this hydroid, the polyp and the medusa, was studied. In situ hybridization demonstrated that expression of the gene corresponding is restricted to secretory cells in the endoderm of the oral hypostome region of polyps and medusae and, presumably, to progenitor cells of this type. Cells expressing Pod-EPPT could not be observed in the larval stage. During head regeneration in polyps, Pod-EPPT expression is upregulated soon after head removal in previously non-expressing cells and in newly differentiating secretory cells. This activation of a head-specific gene precedes the morphologically obvious events of head regeneration. Pod-EPPT is one of the genes that are activated during manubrium (mouth) regeneration from experimentally combined subumbrellar plate endoderm and striated muscle of the medusa.
authors.affiliation: Zoologisches Institut, Universität Basel, CH-4051, Basel, Switzerland.
authors.forename: C D
authors.initials: CD
authors.lastname: Baader
authors.affiliation: Zoologisches Institut, Universität Basel, CH-4051, Basel, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Schuchert
authors.affiliation: Zoologisches Institut, Universität Basel, CH-4051, Basel, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Schmid
authors.affiliation: Institut für Molekulare Neurobiochemie, Ruhruniversität Bochum, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Heiermann
authors.affiliation: Zoologisches Institut, Universität zu Köln, 50923, Köln, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Plickert
keywords: Cell type-specific gene expression; Head regeneration; Pattern formation; Podocoryne carnea; Transdifferentiation