title: Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion.
abstract: Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and Wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to GSIS determination. Conditioned media from insulin-treated versus nontreated myotubes had higher RANTES but lower IL6, IL8, and MCP1 concentration. Qualitative analyses revealed that conditioned media from noninsulin- and insulin-treated myotubes expressed 32 and 23 out of 80 myokines, respectively. Islets incubated with conditioned media from noninsulin-treated myotubes had higher GSIS versus control islets (p< 0.05). Meanwhile, conditioned media from insulin-treated myotubes did not influence GSIS. In beta cells, GSIS was similar across conditions. In conclusion, factors being present in noninsulin-stimulated muscle cell-derived media appear to influence GSIS in mice islets.
authors.affiliation: Departamento de Nutrición, Diabetes y Metabolismo, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Maria L
authors.identifier: 0000-0002-6396-8823
authors.initials: ML
authors.lastname: Mizgier
authors.affiliation: Departamento de Nutrición, Diabetes y Metabolismo, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Luis R
authors.initials: LR
authors.lastname: Cataldo
authors.affiliation: Departamento de Nutrición, Diabetes y Metabolismo, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Juan
authors.initials: J
authors.lastname: Gutierrez
authors.affiliation: Departamento de Nutrición, Diabetes y Metabolismo, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: José L
authors.initials: JL
authors.lastname: Santos
authors.affiliation: Centro de Estudios Moleculares de la Célula, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Mariana
authors.initials: M
authors.lastname: Casas
authors.affiliation: Centro de Estudios Moleculares de la Célula, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile; Institute for Research in Dental Sciences, Facultad de Odontología, Universidad de Chile, Santiago, Chile.
authors.forename: Paola
authors.identifier: 0000-0003-1383-7961
authors.initials: P
authors.lastname: Llanos
authors.affiliation: Exercise Science Laboratory, School of Kinesiology, Faculty of Medicine, Universidad Finis Terrae, Santiago, Chile.
authors.forename: Ariel E
authors.initials: AE
authors.lastname: Contreras-Ferrat
authors.affiliation: INSERM UMR1048, Institut des Maladies Métaboliques et Cardiovasculaires, Université Paul Sabatier, Toulouse, France.
authors.forename: Cedric
authors.initials: C
authors.lastname: Moro
authors.affiliation: Departement de Génétique et Développement, CMU, Université de Genève, Genève, Switzerland; UMR DIATHEC, EA 7294, Centre Européen d'Etude du Diabète, Université de Strasbourg, Strasbourg, France.
authors.forename: Karim
authors.initials: K
authors.lastname: Bouzakri
authors.affiliation: Departamento de Nutrición, Diabetes y Metabolismo, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile; UDA-Ciencias de la Salud, Carrera de Nutrición y Dietética, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Jose E
authors.identifier: 0000-0001-9793-8561
authors.initials: JE
authors.lastname: Galgani
mesh_terms: D000818:Animals; D018946:Chemokine CCL5 / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018946:Chemokine CCL5; D017077:Culture Media, Conditioned; D007328:Insulin; D015850:Interleukin-6; D016209:Interleukin-8; D005947:Glucose