title: Treatment of Plants with Gaseous Ethylene and Gaseous Inhibitors of Ethylene Action.
abstract: The gaseous nature of ethylene affects not only its role in plant biology but also how you treat plants with the hormone. In many ways, it simplifies the treatment problem. Other hormones have to be made up in solution and applied to some part of the plant hoping the hormone will be taken up into the plant and translocated throughout the plant at the desired concentration. Because all plant cells are connected by an intercellular gas space the ethylene concentration you treat with is relatively quickly reached throughout the plant. In some instances, like mature fruit, treatment with ethylene initiates autocatalytic synthesis of ethylene. However, in most experiments, the exogenous ethylene concentration is saturating, usually >1 μL L-1, and the synthesis of additional ethylene is inconsequential. Also facilitating ethylene research compared with other hormones is that there are inhibitors of ethylene action 1-MCP (1-methylcyclopropene) and 2,5-NBD (2,5-norbornadiene) that are also gases wherein you can achieve nearly 100% inhibition of ethylene action quickly and with few side effects. Inhibitors for other plant hormones are applied as a solution and their transport and concentration at the desired site is not always known and difficult to measure. Here, our focus is on how to treat plants and plant parts with the ethylene gas and the gaseous inhibitors of ethylene action.
authors.affiliation: Soybean Genomics and Improvement Lab, USDA/ARS, Bldg 006, BARC-West, 10300 Baltimore Ave, Beltsville, MD, 20705, USA. Mark.Tucker@ars.usda.gov.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Tucker
authors.affiliation: Department of Cell Biology and Molecular Genetics, Biosciences Research Bldg, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Joonyup
authors.initials: J
authors.lastname: Kim
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Chi-Kuang
authors.initials: CK
authors.lastname: Wen
mesh_terms: D003521:Cyclopropanes / Q000494:pharmacology; D005030:Ethylenes / Q000737:chemistry / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000737:chemistry / Q000494:pharmacology*; D010944:Plants / Q000187:drug effects*
chemical_list: D003521:Cyclopropanes; D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene; C412563:1-methylcyclopropene
keywords: 1-MCP; 1-methylcyclopropene; Ethephon; Ethylene; Gaseous treatment; Norbornadiene