title: Isolation of plasma membranes from Xenopus embryos.
abstract: Plasma membranes were isolated in high yield from Xenopus gastrulae by repeated sedimentation in discontinuous sucrose gradients. Most of the yolk was separated by lowspeed sedimentation before centrifugation on the discontinuous sucrose gradients. The isolation of plasma membranes was followed by covalent labelling of the surface of dissociated gastrula cells with diazoniobenzene sulphonate, by electron microscopy and the distribution of enzymatic markers. The isolated plasma membranes have a low neural inducing activity as compared to other cell constituents.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Günther
authors.initials: G
authors.lastname: Bretzel
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Jutta
authors.initials: J
authors.lastname: Janeczek
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Jochen
authors.initials: J
authors.lastname: Born
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Manorama
authors.initials: M
authors.lastname: John
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Heinz
authors.initials: H
authors.lastname: Tiedemann
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Hildegard
authors.initials: H
authors.lastname: Tiedemann
keywords: Amphibian embryogenesis; Neural induction; Plasma membranes; Xenopus laevis