title: Modelling T cell proliferation: Dynamics heterogeneity depending on cell differentiation, age, and genetic background.
abstract: Cell proliferation is the common characteristic of all biological systems. The immune system insures the maintenance of body integrity on the basis of a continuous production of diversified T lymphocytes in the thymus. This involves processes of proliferation, differentiation, selection, death and migration of lymphocytes to peripheral tissues, where proliferation also occurs upon antigen recognition. Quantification of cell proliferation dynamics requires specific experimental methods and mathematical modelling. Here, we assess the impact of genetics and aging on the immune system by investigating the dynamics of proliferation of T lymphocytes across their differentiation through thymus and spleen in mice. Our investigation is based on single-cell multicolour flow cytometry analysis revealing the active incorporation of a thymidine analogue during S phase after pulse-chase-pulse experiments in vivo, versus cell DNA content. A generic mathematical model of state transition simulates through Ordinary Differential Equations (ODEs) the evolution of single cell behaviour during various durations of labelling. It allows us to fit our data, to deduce proliferation rates and estimate cell cycle durations in sub-populations. Our model is simple and flexible and is validated with other durations of pulse/chase experiments. Our results reveal that T cell proliferation is highly heterogeneous but with a specific "signature" that depends upon genetic origins, is specific to cell differentiation stages in thymus and spleen and is altered with age. In conclusion, our model allows us to infer proliferation rates and cell cycle phase durations from complex experimental 5-ethynyl-2'-deoxyuridine (EdU) data, revealing T cell proliferation heterogeneity and specific signatures.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM, Immunology-Immunopathology-Immunotherapy (I3) UMRS959; Paris, France.
authors.forename: Julien
authors.identifier: 0000-0002-2370-3302
authors.initials: J
authors.lastname: Vibert
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM, Immunology-Immunopathology-Immunotherapy (I3) UMRS959; Paris, France.
authors.forename: Véronique
authors.identifier: 0000-0001-7521-8635
authors.initials: V
authors.lastname: Thomas-Vaslin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics* / Q000276:immunology; D003198:Computer Simulation; D000068617:Genetic Background; D055261:Immunogenetic Phenomena / Q000235:genetics; D051379:Mice; D008957:Models, Genetic; D018448:Models, Immunological; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*