title: Novel short antimicrobial peptide isolated from Xenopus laevis skin.
abstract: A rich source of bioactive peptides, including a large number of antimicrobial peptides, has been found in amphibian skin. In this study, a novel short antimicrobial peptide was purified from Xenopus laevis skin and characterised through reversed-phase high-performance liquid chromatography, Edman degradation and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The peptide was composed of six amino acids with a sequence of DEDLDE and thus named X. laevis antibacterial peptide-P2 (XLAsp-P2). Transmission electron microscopy revealed that this peptide showed potential antimicrobial abilities against bacteria by damaging the bacterial cell membrane. XLAsp-P2 maybe inhibit bacterial growth by binding to the microbial genomic DNA. The peptide also exhibited a weak haemolytic activity against rabbit red blood cells. Therefore, XLAsp-P2 is a novel short anionic antibacterial peptide with broad activities. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.|Five-Star Animal Health Pharmaceutical Factory of Jilin Province, Changchun, Jilin Province, 130062, China.
authors.forename: Songcai
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Nie
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Lv
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Wei
authors.affiliation: China National Corp of Traditional and Herbal Medicine Changbaishan Co. Ltd., Yanji, Jilin Province, 133000, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.affiliation: IACA Biological Technology Co. Ltd., Tianjin, 300475, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Hou
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin Province, 130062, China.
authors.forename: Linlin
authors.identifier: http://orcid.org/0000-0001-6629-7722
authors.initials: L
authors.lastname: Hao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002462:Cell Membrane / Q000187:drug effects; D056148:Chromatography, Reverse-Phase; D005260:Female; D008297:Male; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D012867:Skin / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D029867:Xenopus Proteins / Q000737:chemistry*; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides; D029867:Xenopus Proteins
keywords: Xenopus laevis; antimicrobial characterisation; antimicrobial peptides; cell haemolysis