title: Characterization of a new badnavirus from Wisteria sinensis.
abstract: The complete genome sequence of a previously undescribed badnavirus isolated from a wisteria plant exhibiting mosaic and crinkle symptoms in Beijing, China, was determined. The circular double-stranded DNA genome of this virus was 7362 bp in size with four open reading frames (ORFs 1 to 4) on the plus strand. Sequence analysis showed that this virus shared the highest (69%) nucleotide (nt) sequence identity with pagoda yellow mosaic associated virus (PYMAV). In the RT-RNase H region of the ORF-3 encoded polyprotein, this virus shared 74% nt sequence identity with PYMAV. Phylogenetic analysis provided further evidence that the virus identified in this study is a member of a new species in the genus Badnavirus. The name wisteria badnavirus 1 (WBV1) is proposed for this new virus.
authors.affiliation: College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China. lyq@bua.edu.cn.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China.
authors.forename: Congliang
authors.initials: C
authors.lastname: Deng
authors.affiliation: Beijing Plant Protection Station, Beijing, 100029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Chinese Society of Inspection and Quarantine, Beijing, 101312, China. zhouq@bjciq.gov.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D019195:Badnavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D032447:Wisteria / Q000821:virology*
chemical_list: D004279:DNA, Viral