title: Monensin interferes with the determination of the mesodermal cell line in embryos of Patella vulgata.
abstract: In embryos of the equally cleaving marine gastropod Patella vulgata, the mesodermal stem cell is determined during the interval between the fifth and sixth cleavage by means of cellular interactions between one of the four vegetally located macromeres with the overlying animal micromeres. Shortly before and during this interaction phase an extracellular matrix (ECM) is present between the interacting cells. In this study the glycosylation-perturbing ionophore monensin was used to investigate the possible morphogenetic significance of the ECM. Incubation of 32-cell-stage Patella embryos in 10-6M monensin results in radialized embryos in which none of the four macromeres interacts with the overlying animal micromeres. None of the macromeres is determined, therefore, to form mesoderm in such embryos. Trochophore larvae reared from these embryos retain their radial symmetry, as is indicated by the presence of four shell glands and four blastopore- or stomodeum-like invaginations in these larvae. The monensin-treated embryos probably secrete abnormal ECM that does not provide the proper conditions for the blastomeres to stretch and interact with the micromeres. Changes in intracellular ionic concentrations may also be involved.
authors.affiliation: Department of Experimental Zoology, University of Utrecht, Padualaan 8, NL-3584, CH Utrecht, The Netherlands.
authors.forename: Wiel M
authors.initials: WM
authors.lastname: Kühtreiber
authors.affiliation: Department of Experimental Zoology, University of Utrecht, Padualaan 8, NL-3584, CH Utrecht, The Netherlands.
authors.forename: Eveline H
authors.initials: EH
authors.lastname: van Til
authors.affiliation: Department of Experimental Zoology, University of Utrecht, Padualaan 8, NL-3584, CH Utrecht, The Netherlands.
authors.forename: Cees A M
authors.initials: CA
authors.lastname: van Dongen
keywords: Determination; Extracellular matrix; Mesoderm; Mollusca; Monensin