title: In Vivo Crystallization of Three-Domain Cry Toxins.
abstract: Bacillus thuringiensis(Bt) is the most successful, environmentally-friendly, and intensively studied microbial insecticide. The major characteristic of Bt is the production of proteinaceous crystals containing toxins with specific activity against many pests including dipteran, lepidopteran, and coleopteran insects, as well as nematodes, protozoa, flukes, and mites. These crystals allow large quantities of the protein toxins to remain stable in the environment until ingested by a susceptible host. It has been previously established that 135 kDa Cry proteins have a crystallization domain at their C-terminal end. In the absence of this domain, Cry proteins often need helper proteins or other factors for crystallization. In this review, we classify the Cry proteins based on their requirements for crystallization.
authors.affiliation: Department of Biotechnology, Lahore College for Women University, Lahore 54590, Pakistan. rooma.adalat@gmail.com.
authors.forename: Rooma
authors.initials: R
authors.lastname: Adalat
authors.affiliation: Department of Biotechnology, Lahore College for Women University, Lahore 54590, Pakistan. zoologist1pk@yahoo.com.
authors.forename: Faiza
authors.initials: F
authors.lastname: Saleem
authors.affiliation: School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9RH, UK. arshakoori.sbs@pu.edu.pk.
authors.forename: Neil
authors.initials: N
authors.lastname: Crickmore
authors.affiliation: Department of Biotechnology, Lahore College for Women University, Lahore 54590, Pakistan. drsnaz31@hotmail.com.
authors.forename: Shagufta
authors.initials: S
authors.lastname: Naz
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan. arshaksbs@yahoo.com.
authors.forename: Abdul Rauf
authors.initials: AR
authors.lastname: Shakoori
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D004731:Endotoxins / Q000737:chemistry* / Q000235:genetics; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D000072417:Protein Domains
chemical_list: D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins
keywords: Bacillus thuringiensis; C terminal domain; helper protein