title: Regulating miRNA-21 Biogenesis By Bifunctional Small Molecules.
abstract: We report a new strategy to regulate microRNAs (miRNAs) biogenesis by using bifunctional small molecules that consist of a pre-miRNA binding unit connected by a linker to a Dicer inhibiting unit. In this effort, fluorescence polarization-based screening was used to identify neomycin as a pre-miR-21 binding ligand. Although neomycin cannot inhibit miR-21 maturation, linking it to the RNase inhibitor 1 forms the bifunctional conjugate 7A, which inhibits the production of miR-21. We expect that this strategy will be applicable to design other molecules for miRNA regulation.
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , 300 Terrace Street NE, Albuquerque, New Mexico 87131, United States.
authors.forename: Hao
authors.identifier: http://orcid.org/0000-0002-5418-0516
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , 300 Terrace Street NE, Albuquerque, New Mexico 87131, United States.
authors.forename: Umesh
authors.initials: U
authors.lastname: Bhattarai
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , 300 Terrace Street NE, Albuquerque, New Mexico 87131, United States.
authors.forename: Zhi-Fo
authors.initials: ZF
authors.lastname: Guo
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico , 300 Terrace Street NE, Albuquerque, New Mexico 87131, United States.
authors.forename: Fu-Sen
authors.identifier: http://orcid.org/0000-0002-1793-0824
authors.initials: FS
authors.lastname: Liang