title: Effects of traditional medical herbs "minor bupleurum decoction" on the non-specific immune responses of white shrimp (Litopenaeus vannamei).
abstract: This study is investigating the effect of minor bupleurum decoction (Xiao-Chai-Hu decoction) on the non-specific immune response of white shrimp (Litopenaeus vannamei). To determine prophenoloxidase activity (proPO), reactive oxygen species production (ROS), superoxide anion production (O2-), nitric oxide production (NO), phagocytic rate (PR), phagocytic index (PI), superoxide dismutase activity (SOD), total haemocyte count (THC) and differential haemocyte count (DHC). In this experiment, treating with different dosages (0, 0.25, 0.5 and, 1%) of minor bupleurum decoction to detect immune parameters on day 0, 1, 2, 4, 7, 14, 21 and 28. Result is shown that 0.25% treatment significantly enhanced the superoxide dismutase (SOD) activity and, 0.25 and 1% treatment significantly increased the ROS production, nitric oxide (NO) production and phagocytic rate (PR) moreover, 0.5 and 1% treatment induced the proPO activity and superoxide anion (O2-) production. Evidence exactly indicated that minor bupleurum decoction is able to enhance the non-specific immunity responses of white shrimp via in vivo examination.
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan, ROC.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Wu
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan, ROC.
authors.forename: Meng-Chou
authors.initials: MC
authors.lastname: Lee
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan, ROC.
authors.forename: Cheng-Ting
authors.initials: CT
authors.lastname: Huang
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan, ROC.
authors.forename: Tzu-Chi
authors.initials: TC
authors.lastname: Kung
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan, ROC.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan, ROC. Electronic address: fhnan@mail.ntou.edu.tw.
authors.forename: Fan-Hua
authors.initials: FH
authors.lastname: Nan
mesh_terms: D000818:Animals; D029972:Bupleurum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007113:Immunity, Innate / Q000187:drug effects*; D033561:Penaeidae / Q000187:drug effects* / Q000276:immunology*
chemical_list: D004365:Drugs, Chinese Herbal; C550200:xiaochaihu
keywords: Flow cytometry; Litopenaeus vannamei; Minor bupleurum decoction; Non-specific immune response