title: Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle.
abstract: Upon acquirement of pulmonary circulation, the ancestral heart may have been remodelled coincidently with, or accompanied by, the production and rearrangement of progenitor cells. However, the progenitor populations that give rise to the left ventricle (LV) and sinus venosus (SV) are still ambiguous. Here we show that the expression of Secreted frizzled-related protein Sfrp5 in the mouse identifies common progenitors for the outflow tract (OFT), LV, atrium and SV but not the right ventricle (RV). Sfrp5 expression begins at the lateral sides of the cardiac crescent, excluding early differentiating regions, and continues in the venous pole, which gives rise to the SV. Lineage-tracing analysis revealed that descendants of Sfrp5-expressing cells at E7.5 contribute not only to the SV but also to the LV, atria and OFT and are found also in the dorsal splanchnic mesoderm accompanied by the expression of the secondary heart field marker, Islet1. These findings provide insight into the arrangement of cardiac progenitors for systemic circulation.
authors.affiliation: Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima 734-8551, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
authors.affiliation: Department of Mammalian Development, National Institute of Genetics, Mishima 411-8540 Japan.|The Graduate University for Advanced Studies (SOKENDAI), Hayama, Kanagawa 240-0193, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Sakaguchi
authors.affiliation: Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima 734-8551, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kamata
authors.affiliation: Department of Forensic Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima 734-8551, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Nagao
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, 1-3-2 Kagamiyama, Higashi-hiroshima 739-8511, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kikuchi
authors.affiliation: Department of Medicine, School of Medicine UC San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Evans
authors.affiliation: Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima 734-8551, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshizumi
authors.affiliation: Center for Developmental Biology (CDB), RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe 650-0047, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Shimono
authors.affiliation: Department of Mammalian Development, National Institute of Genetics, Mishima 411-8540 Japan.|The Graduate University for Advanced Studies (SOKENDAI), Hayama, Kanagawa 240-0193, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saga
authors.affiliation: Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima 734-8551, Japan.
authors.forename: Hiroki
authors.identifier: 0000-0002-7216-3066
authors.initials: H
authors.lastname: Kokubo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019521:Body Patterning / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D058948:Cell Tracking / Q000379:methods; D054326:Coronary Sinus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D004622:Embryo, Mammalian; D051526:Fibroblast Growth Factor 10 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006325:Heart Atria / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D006352:Heart Ventricles / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D064326:MEF2 Transcription Factors / Q000235:genetics / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D051526:Fibroblast Growth Factor 10; D036341:Intercellular Signaling Peptides and Proteins; D060850:LIM-Homeodomain Proteins; D064326:MEF2 Transcription Factors; D020825:T-Box Domain Proteins; D014157:Transcription Factors; C496464:Fgf10 protein, mouse; C578041:Mef2c protein, mouse; C487972:Sfrp5 protein, mouse; D000094742:Secreted Frizzled-Related Proteins; C491573:Tbx1 protein, mouse; C063843:insulin gene enhancer binding protein Isl-1