title: Characterization of hsa_circ_0004277 as a New Biomarker for Acute Myeloid Leukemia via Circular RNA Profile and Bioinformatics Analysis.
abstract: Circular RNAs (circRNAs) represent a widespread class of non-coding RNAs, which drew little attention in the past. Recently, limited data showed their promising future to act as biomarkers in human cancer, but the characteristics and functions remain largely unknown in hematopoietic malignancies, especially in leukemia. In this study, with the help of circRNA microarray, we demonstrated the expression profile of circRNAs in acute myeloid leukemia (AML) patients, and identified a large number of circRNAs possibly expressed in a leukemia specific manner. We also described a circRNA signature related to AML risk-status based on the bioinformatics prediction. In particular, a downregulated circRNA, hsa_circ_0004277, was characterized and functionally evaluated in a cohort of 115 human samples, thus offering a potential diagnostic marker and treatment target in AML. Interestingly, we found chemotherapy could significantly restore the expression of hsa_circ_0004277, indicating the increasing level of hsa_circ_0004277 was associated with successful treatment. Furthermore, a detailed circRNA-miRNA-mRNA interaction network was presented for hsa_circ_0004277, allowing us to better understand its underlying mechanisms for function in AML.
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China. liwei_medicine@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China. zhongchq07@126.com.
authors.forename: Chaoqin
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan 250012, China. jiaojun206@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China. pengli85@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan 250012, China. cuibaoxia@sdu.edu.cn.
authors.forename: Baoxia
authors.initials: B
authors.lastname: Cui
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China. jichunyan@sdu.edu.cn.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Ji
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China. daoxinma@sdu.edu.cn.
authors.forename: Daoxin
authors.initials: D
authors.lastname: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D000079962:RNA, Circular
chemical_list: D014408:Biomarkers, Tumor; D000079962:RNA, Circular; D012313:RNA
keywords: AML; biomarker; circRNAs; hsa_circ_0004277