title: rs35301225 polymorphism in miR-34a promotes development of human colon cancer by deregulation of 3'UTR in E2F1 in Chinese population.
abstract: BACKGROUND
  Previous reports have revealed that down-regulation of miR-34a expression can promote colorectal cancer (CRC) cell growth by targeting cell cycle-related transcriptional factor E2F1. To date, the function of the single nucleotide polymorphism (SNP) located in the mature region of miR-34a has not been investigated.
  
  
  METHODS
  We performed a case-control study including 685 CRC patients and 618 cancer-free controls. Genotyping, real-time PCR assay, cell transfection, and the dual luciferase reporter assay were used in our study. Cell proliferation and cell cycle analysis were measured in CRC cells including Hct-116 and SW480. The overall survival of different genotypes was also investigated.
  
  
  RESULTS
  We found that the rs35301225 polymorphism in miR-34a was involved in the occurrence of CRC by acting as a tumor suppressor by down-regulation of tumor-promoting gene E2F1. C/A SNP of miR-34a could promote CRC cell proliferation by up-regulation of E2F1. Also, C/A genotype can change the cell cycle by increasing the S phase percentage. Moreover, the SNP in rs35301225 of miR-34a was associated with tumor size and tumor differentiation, as well as metastasis in CRC patients; C/A SNP was related to the significantly enhanced expression of E2F1 and shorter survival in post-surgery CRC patients.
  
  
  CONCLUSIONS
  rs35301225 in miR-34a was highly associated with a decreased risk of CRC in a Chinese population and might serve as a novel biomarker for colon cancer.
authors.affiliation: Department of Clinical Laboratory, Jiangyin Hospital of Chinese Traditional Medicine, Wuxi, China.
authors.forename: Haiqiang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, Jiangyin Hospital of Chinese Traditional Medicine, Wuxi, China.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Ge
authors.affiliation: Department of Clinical Laboratory, Jiangyin Hospital of Chinese Traditional Medicine, Wuxi, China.
authors.forename: Beina
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory, Anting Hospital, Shanghai, China.
authors.forename: Lamei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Central Hospital of Jiading District, Shanghai, China.
authors.forename: Huijian
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Clinical Laboratory, Jiangyin Hospital of Chinese Traditional Medicine, Wuxi, China.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Wang
keywords: E2F1; Genotype; SNP; Tumor growth; miR-34a