title: Development of Urinary Bladder Pre-Neoplasia bySchistosoma haematobiumEggs and Chemical Carcinogen in Mice.
abstract: Schistosoma haematobiumis a biocarcinogen of human urinary bladder (UB). The present study investigated developing UB cancer mouse model by injectingS. haematobiumeggs into the bladder wall and introduction of chemical carcinogens. Histopathological findings showed mild hyperplasia to epithelial vacuolar change, and high grade dysplasia. Squamous metaplasia was observed in theS. haematobiumeggs+NDMA group at week 12 but not in other groups. Immunohistochemistry revealed significantly high expression ofKi-67in urothelial epithelial cells of theS. haematobiumeggs+BBN group at week 20. The qRT-PCR showed high expression ofp53gene inS. haematobiumeggs group at week 4 andS. haematobiumeggs+BBN group at week 20.E-cadherinandvimentinshowed contrasting expression inS. haematobiumeggs+BBN group. Such inverse expression ofE-cadherinandvimentinmay indicate epithelial mesenchymal transition in the UB tissue. In conclusion,S. haematobiumeggs and nitrosamines may transform UB cells into squamous metaplasia and dysplasia in correlation with increased expression of Ki-67. Marked decrease inE-cadherinand increase inp53andvimentinexpressions may support the transformation. The present study introduces a promising modified animal model for UB cancer study usingS. haematobiumeggs.
authors.affiliation: Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Bayissa
authors.initials: B
authors.lastname: Chala
authors.affiliation: Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Choi
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Kyung Chul
authors.initials: KC
authors.lastname: Moon
authors.affiliation: Department of Urology, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Hyung Suk
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Urology, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Cheol
authors.initials: C
authors.lastname: Kwak
authors.affiliation: Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Hong
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004195:Disease Models, Animal; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008813:Mice, Inbred ICR; D008853:Microscopy; D011230:Precancerous Conditions / Q000139:chemically induced / Q000469:parasitology / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D012548:Schistosoma haematobium / Q000472:pathogenicity*; D012553:Schistosomiasis haematobia / Q000150:complications*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000469:parasitology / Q000473:pathology*
chemical_list: D002273:Carcinogens; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53
keywords: N-butyl n-(4-hydroxybutyl) nitrosamine; N-nitrosodimethylamine; Schistosoma haematobium; bladder cancer; egg; mouse