title: Complete genome sequence ofPseudomonas citronellolisP3B5, a candidate for microbial phyllo-remediation of hydrocarbon-contaminated sites.
abstract: Pseudomonas citronellolisis a Gram negative, motile gammaproteobacterium belonging to the orderPseudomonadalesand the familyPseudomonadaceae. We isolated strain P3B5 from the phyllosphere of basil plants (Ocimum basilicumL.). Here we describe the physiology of this microorganism, its full genome sequence, and detailed annotation. The 6.95 Mbp genome contains 6071 predicted protein coding sequences and 96 RNA coding sequences.P. citronellolishas been the subject of many studies including the investigation of long-chain aliphatic compounds and terpene degradation. Plant leaves are covered by long-chain aliphates making up a waxy layer that is associated with the leaf cuticle. In addition, basil leaves are known to contain high amounts of terpenoid substances, hinting to a potential nutrient niche that might be exploited byP. citronellolis. Furthermore, the isolated strain exhibited resistance to several antibiotics. To evaluate the potential of this strain as source of transferable antibiotic resistance genes on raw consumed herbs we therefore investigated if those resistances are encoded on mobile genetic elements. The availability of the genome will be helpful for comparative genomics of the phylogenetically broad pseudomonads, in particular with the sequence of theP. citronellolistype strain PRJDB205 not yet publicly available. The genome is discussed with respect to a phyllosphere related lifestyle, aliphate and terpenoid degradation, and antibiotic resistance.
authors.affiliation: Agroscope, Institute for Food Sciences IFS, Wädenswil, Switzerland.
authors.forename: Mitja N P
authors.initials: MNP
authors.lastname: Remus-Emsermann
authors.affiliation: Agroscope, Institute for Plant Production Sciences IPS, Wädenswil, Switzerland.|Swiss Institute of Bioinformatics, Wädenswil, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmid
authors.affiliation: Agroscope, Institute for Food Sciences IFS, Wädenswil, Switzerland.|ETH Zurich, Institute of Food, Nutrition and Health, Zurich, Switzerland.
authors.forename: Maria-Theresia
authors.initials: MT
authors.lastname: Gekenidis
authors.affiliation: Agroscope, Institute for Plant Production Sciences IPS, Wädenswil, Switzerland.
authors.forename: Cosima
authors.initials: C
authors.lastname: Pelludat
authors.affiliation: Agroscope, Institute for Plant Production Sciences IPS, Wädenswil, Switzerland.
authors.forename: Jürg E
authors.initials: JE
authors.lastname: Frey
authors.affiliation: Agroscope, Institute for Plant Production Sciences IPS, Wädenswil, Switzerland.|Swiss Institute of Bioinformatics, Wädenswil, Switzerland.
authors.forename: Christian H
authors.initials: CH
authors.lastname: Ahrens
authors.affiliation: Agroscope, Institute for Food Sciences IFS, Wädenswil, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Drissner
keywords: Alkane degradation; Bioremediation potential; Complete genome; Nonhybrid de novo assembly; PacBio; Phyllosphere; Pseudomonad; Terpenoid degradation