title: Dysbiosis of Gut Microbiota Associated with Clinical Parameters in Polycystic Ovary Syndrome.
abstract: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. Gut microbiota has been implicated to play a critical role in metabolic diseases and may modulate the secretion of mediators of the brain-gut axis. Interaction between gut microbiota and the endocrine and biochemical disturbances in PCOS still remains elusive. Here, we showed an altered gut microbiota significantly correlated with PCOS phenotype. There were 33 patients with PCOS (non-obese PCOS individuals, PN,n= 12; obese PCOS individuals, PO,n= 21) as well as 15 control subjects (non-obese control individuals, CN,n= 9; obese control individuals, CO,n= 6) enrolled in our study. The plasma levels of serotonin, ghrelin, and peptide YY (PYY) were significantly decreased in patients with PCOS compared with controls, and have a significantly negative correlation with waist circumference and testosterone. Sequencing of the V3-V4 region of the 16S rRNA gene in fecal samples revealed the substantial differences of gut microbial species between the PCOS and non-obese controls. Bacterial species were clustered into 23 co-abundance groups (CAGs) based on the SparCC correlation coefficients of their relative abundance. The CAGs increased in PCOS, including the bacteria belonging toBacteroides, Escherichia/ShigellaandStreptococcus, were negatively correlated with ghrelin, and positively correlated with testosterone and BMI. Furthermore, the CAGs that were decreased in PCOS, including the bacteria fromAkkermansiaand Ruminococcaceae, showed opposite relationship with body-weight, sex-hormone, and brain-gut peptides. In conclusion, gut microbial dysbiosis in women with PCOS is associated with the disease phenotypes.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Chenhong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology and Metabolism, Qidong People's Hospital Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Linxia
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Huaqing
authors.initials: H
authors.lastname: Fu
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.affiliation: Obesity and Metabolism Laboratory, Jean-Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston MA, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Reeves
authors.affiliation: Obesity and Metabolism Laboratory, Jean-Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston MA, USA.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Greenberg
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Yongde
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Ding
keywords: ghrelin; gut microbiota; obesity; polycystic ovary syndrome; serotonin; testosterone