title: Electrophoretic and histochemical analysis of esterase synthesis in the ovariole of the milkweed bugOncopeltus fasciatus (Dall.).
abstract: The synthesis of esterases was analysed in the ovariole of the milkweed bug using electrophoretic and histochemical methods. We found a total of 12 esterases from whole ovaries. Using the inhibitors p-hydroxymercuribenzoate, diisopropyl phosphorofluoridate and eserine, 3 of the bands were classed as acetylesterases, 7 carboxylesterases, and 2 arylesterases. No change in esterase patterns occurred during oogenesis.The esterase activity in the follicular epithelium was primarily arylesterase and carboxylesterase. A carboxylesterase was also identified from the central region of the follicle cell. The yolk cells contained mainly carboxylesterase. There was an apparent activation of enzyme activity following pre-treatment with eserine.
authors.affiliation: Department of Zoology, University of Illinois, USA.
authors.forename: P
authors.initials: P
authors.lastname: Cho
authors.affiliation: Department of Zoology, University of Illinois, USA.
authors.forename: R
authors.initials: R
authors.lastname: Davenport
authors.affiliation: Department of Zoology, University of Illinois, USA.
authors.forename: G S
authors.initials: GS
authors.lastname: Whitt
authors.affiliation: Department of Entomology, University of Illinois, 61801, Urbana, Illinois.|Illinois Natural History Survey, Room 163, Natural Resources Building, 61801, Urbana, Illinois.
authors.forename: G M
authors.initials: GM
authors.lastname: Booth
authors.affiliation: Department of Entomology, University of Illinois, 61801, Urbana, Illinois.
authors.forename: J R
authors.initials: JR
authors.lastname: Larsen