title: A Study to Investigate the Role ofGULP/ CED 6Gene in "Eat Me" Signaling in Cellular Efferocytosis and Immunosurveillance.
abstract: In this report, investigations were done to study humanGULP/ CED 6genes role in presenting cancer cells to scavenger cells. CED 6 SiRNA was used to knock out the gene in Astrocytoma (HTB-12) cell lines to study its effects on expression of various "eat me" signals on these cells including Phosphatidyl serine (PtdSer) expression, nitric oxide (NO) signaling and Leukotrine B4 (LTB4) expression and Caspase 3 activation. Investigations were done by fluorescence microscopy techniques, ELISA assay and colorimetric assays using a standard microplate reader and spectrophotometer. Initial results showed all the above mentioned "eat me" signals were significantly decreased in CED 6 knock out cell lines. ThereforeCED 6gene must have a role in cancer cell clearance, pathway involved in the cross talk betweenCED 6and other genes in this process is a matter of farther investigation.
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: H
authors.initials: H
authors.lastname: Banerjee
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: B
authors.initials: B
authors.lastname: Bazemore
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: A
authors.initials: A
authors.lastname: Barfield
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: D
authors.initials: D
authors.lastname: Crummity
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: C
authors.initials: C
authors.lastname: Krauss
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: G
authors.initials: G
authors.lastname: Payne
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: J
authors.initials: J
authors.lastname: Rousch
authors.affiliation: Department of Natural Pharmacy and Health Sciences, Elizabeth City State University Campus, University of North Carolina, Elizabeth City, USA.
authors.forename: V
authors.initials: V
authors.lastname: Manglik
keywords: Astrocytoma; Fluorescence; Scavenger cells; Spectrophotometer