title: Mutation pattern in the genome ofNeisseria gonorrhoeaeand its association with multidrug-resistant isolates from Delhi, India.
abstract: Mutations in PenA, PorB, MtrE and MtrR genes responsible for antimicrobial resistance were checked in 27 drug-resistant clinical isolates of Neisseria gonorrhoeae (NG). Phenotype PIB (88.88%) and mutation at G120 and A121 positions of porB were recurrent. N122K, a novel mutation, was observed in PorB in three resistant isolates. Substitution H105Y in MtrR was widespread (37% of clinical isolates). The presence of a novel mutation (L33V), along with G45D mutation in MtrR, was associated with less-resistant isolates, in contrast to isolates with G45D mutation alone. African-type penicillinase-producing NG plasmid was observed most frequently (17/27) in penicillin-resistant isolates.
authors.affiliation: Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, New Delhi, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Sachdev
authors.affiliation: Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, New Delhi, India.
authors.forename: Indu
authors.initials: I
authors.lastname: Kumari
authors.affiliation: Training and Research Centre, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Bala
authors.affiliation: Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, New Delhi, India.
authors.forename: Vineet
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, New Delhi, India.
authors.forename: Daman
authors.initials: D
authors.lastname: Saluja
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006801:Humans; D007194:India; D008297:Male; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins