title: Sulforaphane attenuates di-N-butylphthalate-induced reproductive damage in pubertal mice: Involvement of the Nrf2-antioxidant system.
abstract: di-N-butylphthalate (DBP) is a ubiquitous environmental pollutant used for plastic coating and in the cosmetics industry. It has toxic effects on body health, especially the male reproductive system. Here, we investigated the effects of DBP on the male reproductive system of pubertal mice and explored the protective role of sulforaphane (SFN). The results showed that DBP significantly reduced the anogenital distance, testicular weight, sperm count and motility, and plasma and testicular testosterone levels and significantly increased the oxidative stress, sperm abnormalities, and testicular cell apoptosis. SFN supplementation ameliorated these effects. After DBP stimulation, the transcription factor nuclear factor erythroid-related factor 2 (Nrf2) was adaptively increased together with its target genes, such as HO-1 and NQO1. Upregulation of Nrf2 by SFN reduced the DBP-mediated intracellular oxidative toxicity and also increased testosterone secretion and spermatogenesis, which were decreased by DBP. These findings indicate that SFN can attenuate DBP-induced reproductive damage in pubertal mice via Nrf2-associated pathways.
authors.affiliation: Department of Urology, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, China.|Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Xu-Ping
authors.initials: XP
authors.lastname: Jiang
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.|Department of Urology, Wuxi Second People's Hospital, Nanjing Medical University, Wuxi, 214002, China.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Tang
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Han
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Qin
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Cheng-di
authors.initials: CD
authors.lastname: Yang
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Shang-Qian
authors.initials: SQ
authors.lastname: Wang
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Urology and Andrology, Drum Tower Hospital, Nanjing, 210008, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Urology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China.
authors.forename: Bai-Xin
authors.initials: BX
authors.lastname: Shen
authors.affiliation: Department of Urology, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhou
authors.affiliation: Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D003993:Dibutyl Phthalate / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D017879:Isothiocyanates / Q000494:pharmacology*; D008297:Male; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013454:Sulfoxides; D013737:Testis / Q000166:cytology / Q000187:drug effects; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D004785:Environmental Pollutants; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; D013454:Sulfoxides; D003993:Dibutyl Phthalate; C016766:sulforaphane
keywords: Nrf2; di-N-butylphthalate; oxidative stress; sulforaphane