title: Effect of azithromycin on gingival overgrowth induced by cyclosporine A + nifedipine combination therapy: A morphometric analysis in rats.
abstract: BACKGROUND
  Drug-induced gingival overgrowth (DIGO) is a well-known adverse effect of cyclosporine A (CsA) and nifedipine (Nf) therapy. The aim of the present morphometric study was to evaluate the effect of azithromycin (Azi) on the combined GO in rats induced by CsA + Nf combination.
  
  
  METHODS
  Thirty Sprague-Dawley male rats were randomly divided equally into three groups. Group 1 (control) received olive oil only; Group 2 received a combination of CsA and Nf in olive oil throughout the study period; Group 3 received CsA + Nf combination therapy, and Azi was added for 1 week in the 5thweek. All the drugs were delivered by oral route. Impressions of the mandibular central incisal regions were taken, and study models were prepared at baseline and biweekly up to the 8 weeks. Statistical analysis was done by one-way analysis of variance and intergroup comparisons were made using Tukey'spost hocanalysis.
  
  
  RESULTS
  Significant GO was evident in Group 2 and Group 3 rats when compared to Group 1. However, in Group 3 (Azi), GO was observed up to the 4thweek, but a significant decrease in GO was noticed during 6-8thweek after the administration of Azi in 5thweek.
  
  
  CONCLUSIONS
  Azi is an effective drug in the remission of DIGO induced by combined therapy of CsA + Nf and thereby can be considered as a useful therapeutic regimen in minimizing the DIGO in transplant patients.
authors.affiliation: Department of Periodontology, Government College of Dentistry, Indore, Madhya Pradesh, India.
authors.forename: Madhu Singh
authors.initials: MS
authors.lastname: Ratre
authors.affiliation: Department of Periodontology, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
authors.forename: Dhoom Singh
authors.initials: DS
authors.lastname: Mehta
keywords: Animal; azithromycin; cyclosporine; gingival overgrowth; model; nifedipine