title: Gold and Hairpin DNA Functionalization of Upconversion Nanocrystals for Imaging and In Vivo Drug Delivery.
abstract: Although multifunctional upconversion imaging probes have recently attracted considerable interest in biomedical research, there are currently few methods for stabilizing these luminescent nanoprobes with oligonucleotides in biological systems. Herein, a method to robustly disperse upconversion nanoprobes in physiological buffers based on rational design and synthesis of nanoconjugates comprising hairpin-DNA-modified gold nanoparticles is presented. This approach imparts the upconversion nanoprobes with excellent biocompatibility and circumvents the problem of particle agglomeration. By combining single-band anti-Stokes near-infrared emission and the photothermal effect mediated by the coupling of gold to upconversion nanoparticles, a simple, versatile nanoparticulate system for simultaneous deep-tissue imaging and drug molecule release in vivo is demonstrated.
authors.affiliation: Department of Orthopedic Surgery, National University of Singapore, Singapore, 119228, Singapore.|Department of Chemistry, National University of Singapore, Singapore, 117543, Singapore.
authors.forename: Sanyang
authors.initials: S
authors.lastname: Han
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore, 138667, Singapore.
authors.forename: Animesh
authors.initials: A
authors.lastname: Samanta
authors.affiliation: Key Laboratory of Flexible Electronics & Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, 211816, P. R. China.
authors.forename: Xiaoji
authors.initials: X
authors.lastname: Xie
authors.affiliation: Key Laboratory of Flexible Electronics & Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, 211816, P. R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore, 138667, Singapore.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Peng
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore, 138667, Singapore.
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Singapore Institute of Neurotechnology (SINAPSE), Singapore, 117456, Singapore.
authors.forename: Daniel Boon Loong
authors.initials: DBL
authors.lastname: Teh
authors.affiliation: Molecular Imaging & Therapy Branch, National Cancer Center, Gyeonggi-do, 10408, Republic of Korea.
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore, 138667, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.affiliation: Department of Orthopedic Surgery, National University of Singapore, Singapore, 119228, Singapore.|Singapore Institute of Neurotechnology (SINAPSE), Singapore, 117456, Singapore.|Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
authors.forename: Angelo Homayoun
authors.initials: AH
authors.lastname: All
authors.affiliation: Division of Chemistry and Biological Chemistry, Nanyang Technological University, Singapore, 637371, Singapore.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Division of Chemistry and Biological Chemistry, Nanyang Technological University, Singapore, 637371, Singapore.
authors.forename: Bengang
authors.initials: B
authors.lastname: Xing
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore, 117543, Singapore.|SZU-NUS Collaborative Innovation Center for Optoelectronic Science & Technology, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, P. R. China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Liu
mesh_terms: D004247:DNA; D016503:Drug Delivery Systems; D006046:Gold; D049449:Luminescence; D053768:Metal Nanoparticles; D009690:Nucleic Acid Conformation
chemical_list: D006046:Gold; D004247:DNA
keywords: deep tissue imaging; drug delivery; hairpin DNA; surface functionalization; upconversion nanoprobes