title: Sequential Injection Chromatography with an Ultra-short Monolithic Column for the Low-Pressure Separation of α-Tocopherol and γ-Oryzanol in Vegetable Oils and Nutrition Supplements.
abstract: A low-pressure separation procedure of α-tocopherol and γ-oryzanol was developed based on a sequential injection chromatography (SIC) system coupled with an ultra-short (5 mm) C-18 monolithic column, as a lower cost and more compact alternative to the HPLC system. A green sample preparation, dilution with a small amount of hexane followed by liquid-liquid extraction with 80% ethanol, was proposed. Very good separation resolution (Rs= 3.26), a satisfactory separation time (10 min) and a total run time including column equilibration (16 min) were achieved. The linear working range was found to be 0.4 - 40 μg with R2being more than 0.99. The detection limits of both analytes were 0.28 μg with the repeatability within 5% RSD (n = 7). Quantitative analyses of the two analytes in vegetable oil and nutrition supplement samples, using the proposed SIC method, agree well with the results from HPLC.
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University.
authors.forename: Sujitra
authors.initials: S
authors.lastname: Thaithet
authors.forename: Supaporn
authors.initials: S
authors.lastname: Kradtap Hartwell
authors.forename: Somchai
authors.initials: S
authors.lastname: Lapanantnoppakhun
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D019587:Dietary Supplements; D010666:Phenylpropionates / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000032:analysis*
chemical_list: D010666:Phenylpropionates; D010938:Plant Oils; D024502:alpha-Tocopherol; C013172:gamma-oryzanol