title: Centrifugation of 2-cell mouse ova: cytoplasm stratification and recovery.
abstract: Two-cell mouse ova, which were centrifuged for l h at 70 000-90 000xg, showed a precise stratification of the cytoplasm and an elongation of the nucleus. The ova were fixed at different times and observed by light and electron microscopy using cytochemical methods and detergent extractions. Within 40 min after centrifugation the normal-looking morphology was recovered except for the persisting lipid caps at the centripetal poles of the blastomeres. Cleavage, compaction and blastulation were not prevented by centrifugation. Treatments with colcemid or cytochalasin D delayed but did not impair recovery. These results suggest that a resilient cytoskeletal structure may be involved in this kind of embryonic regulation.
authors.affiliation: Departamento de Biologia, Universidad de Chile, Casilla, 653, Santiago, Chile.
authors.forename: Verónica
authors.initials: V
authors.lastname: Téllez
authors.affiliation: Departamento de Biologia, Universidad de Chile, Casilla, 653, Santiago, Chile.
authors.forename: Ariel
authors.initials: A
authors.lastname: Ahumada
authors.affiliation: Departamento de Biologia, Universidad de Chile, Casilla, 653, Santiago, Chile.
authors.forename: Juan
authors.initials: J
authors.lastname: Muro
authors.affiliation: Departamento de Biologia, Universidad de Chile, Casilla, 653, Santiago, Chile.
authors.forename: Soledad
authors.initials: S
authors.lastname: Sepúlveda
authors.affiliation: Departamento de Biologia, Universidad de Chile, Casilla, 653, Santiago, Chile.
authors.forename: Luis
authors.initials: L
authors.lastname: Izquierdo
keywords: Centrifugation; Cytoskeleton; Mouse ova; Regulation