title: Amino acid transport and protein synthesis in induced ectoderm of amphibian embryos.
abstract: Isolated gastrula ectoderm ofTriturus alpestris orAmbystoma mexicanum was induced by the vegetalizing factor. Protein synthesis in the induced and uninduced control explants was measured by double labelling with3H-and14C-amino acids after different periods of cultivation. Slight differences were observed in the pattern of nuclear proteins after 12 h of cultivation and in the pattern of cytoplasmic proteins after 48 h of cultivation.The uptake of leucine started to increase in induced explants after 48 h of cultivation and after 96 h was about 50 times greater than in uninduced control explants. The uptake is reduced under partially anaerobic conditions. Ouabain inhibits the uptake by about 50%.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, 1000, Berlin 33.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Minuth
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, 1000, Berlin 33.
authors.forename: Maria
authors.initials: M
authors.lastname: Minuth
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, 1000, Berlin 33.
authors.forename: Heinz
authors.initials: H
authors.lastname: Tiedemann
keywords: Amino acid transport; Amphibian ectoderm; Induction; Protein synthesis