title: Th17 and IL-17 Cause Acceleration of Inflammation and Fat Loss by Inducing α2-Glycoprotein 1 (AZGP1) in Rheumatoid Arthritis with High-Fat Diet.
abstract: Rheumatoid arthritis (RA) is a chronic autoimmune disorder that affects the joints. High-fat diet (HFD) is a risk factor for RA and is related to inflammation but responds minimally to medication. Given the association between HFD and inflammation, it is important to understand the function of inflammation-related T cells in RA with HFD. Collagen-induced arthritis (CIA), a model of RA, was induced in HFD mice by injection of collagen II, and metabolic markers and T cells were analyzed. The metabolic index and IgG assay results were higher in HFD-CIA mice than in nonfat diet-CIA mice. Numbers of inflammation-related T cells and macrophages, such as Th1 and Th17 cells and M1 macrophages, were higher in spleens of HFD-CIA mice. HFD-CIA mice had a high level of α2-glycoprotein 1 (Azgp1), a soluble protein that stimulates lipolysis. To examine the association between Azgp1 and Th17 cells, the reciprocal effects of Azgp1 and IL-17 on Th17 differentiation and lipid metabolism were measured. Interestingly, Azgp1 increased the Th17 population of splenocytes. Taken together, our data suggest that the acceleration of fat loss caused by Azgp1 in RA with metabolic syndrome is related to the increase of IL-17. Mice injected with the Azgp1-overexpression vector exhibited more severe CIA compared with the mock vector-injected mice.
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hyun Sik
authors.initials: HS
authors.lastname: Na
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Kwon
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: JooYeon
authors.initials: J
authors.lastname: Jhun
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.affiliation: IMPACT Biotech, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: KyungAh
authors.initials: K
authors.lastname: Jung
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Center for Rheumatic Disease, Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Park
authors.affiliation: Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; Laboratory of Immune Network, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea; IMPACT Biotech, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: iammila@catholic.ac.kr.
authors.forename: Mi-La
authors.initials: ML
authors.lastname: Cho
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced; D001172:Arthritis, Rheumatoid / Q000209:etiology*; D002454:Cell Differentiation / Q000502:physiology; D024043:Collagen Type II / Q000633:toxicity; D059305:Diet, High-Fat / Q000009:adverse effects*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D020381:Interleukin-17 / Q000494:pharmacology / Q000502:physiology*; D050356:Lipid Metabolism / Q000502:physiology; D008297:Male; D008659:Metabolic Diseases / Q000503:physiopathology; D008811:Mice, Inbred DBA; D011994:Recombinant Proteins / Q000494:pharmacology; D029589:Seminal Plasma Proteins / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000502:physiology; D058504:Th17 Cells / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D024043:Collagen Type II; D007136:Immunoglobulins; D020381:Interleukin-17; D011994:Recombinant Proteins; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein; D006003:Glycogen