title: A novel model to assess lamellar signaling relevant to preferential weight bearing in the horse.
abstract: Supporting limb laminitis (SLL) is a devastating sequela to severe unilateral lameness in equine patients. The manifestation of SLL, which usually only affects one limb, is unpredictable and the etiology is unknown. A novel, non-painful preferential weight bearing model designed to mimic the effects of severe unilateral forelimb lameness was developed to assess lamellar signaling events in the supporting limb (SL). A custom v-shaped insert was attached to the shoe of one forelimb to prevent normal weight bearing and redistribute weight onto the SL. Testing of the insert using a custom scale platform built into the floor of stocks confirmed increased distribution of weight on the SL compared with the unloaded forelimb (UL) and the contralateral (CH) and ipsilateral (IH) hind limbs in six Standardbred horses. In a second part of the study, eight healthy Standardbred horses were fitted with the insert and tied with consistent monitoring and free access to hay and water for 48 h, after which the lamellae were harvested. Real-time qPCR was performed to assess lamellar mRNA concentrations of inflammatory genes and immunoblotting and immunofluorescence were performed to assess lamellar protein concentration and cellular localization of hypoxia-related proteins, respectively. Lamellar mRNA concentrations of inflammatory signaling proteins did not differ between SL and either CH or IH samples. HIF-1α concentrations were greater (P < 0.05) in the SL compared to the CH. This work establishes an experimental model to study preferential weight bearing and initial results suggest that lamellar hypoxia may occur in the SL.
authors.affiliation: College of Veterinary Medicine, The Ohio State University, 601 Vernon L. Tharp Street, Columbus, OH 43201, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Gardner
authors.affiliation: School of Veterinary Science, The University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia.
authors.forename: A W
authors.initials: AW
authors.lastname: van Eps
authors.affiliation: College of Veterinary Medicine, The Ohio State University, 601 Vernon L. Tharp Street, Columbus, OH 43201, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Watts
authors.affiliation: College of Veterinary Medicine, The Ohio State University, 601 Vernon L. Tharp Street, Columbus, OH 43201, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Burns
authors.affiliation: College of Veterinary Medicine, The Ohio State University, 601 Vernon L. Tharp Street, Columbus, OH 43201, USA. Electronic address: belknap.16@osu.edu.
authors.forename: J K
authors.initials: JK
authors.lastname: Belknap
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D005534:Foot Diseases / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006724:Hoof and Claw / Q000503:physiopathology; D006734:Horse Diseases / Q000175:diagnosis* / Q000503:physiopathology; D006736:Horses; D015151:Immunoblotting / Q000662:veterinary; D008297:Male; D008954:Models, Biological; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D015398:Signal Transduction / Q000502:physiology; D016474:Weight-Bearing / Q000502:physiology*; D001613:beta 2-Microglobulin / Q000378:metabolism
chemical_list: D000199:Actins; D001613:beta 2-Microglobulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
keywords: Cell signaling; HIF-1α; Hypoxia; Preferential weight bearing; Supporting limb laminitis