title: MiR-338-5p suppresses proliferation, migration, invasion, and promote apoptosis of glioblastoma cells by directly targeting EFEMP1.
abstract: OBJECTIVE
  We aimed to investigate the effect of miR-338-5p on proliferation, migration and invasion of glioblastoma (GBM) cells by regulating EFEMP1.
  
  
  METHODS
  The expression of miR-338-5p and EFEMP1 was measured by qRT-PCR and western blot. Transfection was conducted to regulate the expression of miR-338-5p and EFEMP1 in U87 cell lines. Cell proliferation, apoptosis, migration and invasion were evaluated using CCK-8 assay, flow cytometry and Transwell assay respectively. Dual luciferase reporter assay was performed to verify whether miR-338-5p directly targeted EFEMP1.
  
  
  RESULTS
  MiR-338-5p was significantly down-regulated in human GBM tumor tissues and cells while EFEMP1 was strongly upregulated (P<0.05). Upregulated miR-338-5p was able to suppress cell proliferation, migration, invasion, and promote cell apoptosis in GBM cells (P<0.05). Dual luciferase reporter gene assay determined that miR-338-5p directly targeted EFEMP1 (P<0.05).
  
  
  CONCLUSIONS
  MiR-338-5p suppressed proliferation, migration and invasion of GBM cells through inhibiting EFEMP1.
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China. Electronic address: liu_haiyi@126.com.
authors.forename: Deqiang
authors.initials: D
authors.lastname: Lei
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
authors.forename: Fangcheng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
authors.forename: Dongxiao
authors.initials: D
authors.lastname: Yao
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
authors.forename: Nanxiang
authors.initials: N
authors.lastname: Xiong
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000503:physiopathology
chemical_list: C119630:EFEMP1 protein, human; D016326:Extracellular Matrix Proteins; C532050:MIRN338 microRNA, human; D035683:MicroRNAs
keywords: EFEMP1; Glioblastoma; MiR-338-5p; Progression