title: MALDI mass spectrometry reveals that cumulus cells modulate the lipid profile of in vitro-matured bovine oocytes.
abstract: The influence of cumulus cells (CC) on the lipid profile of bovine oocytes matured in two different lipid sources was investigated. Cumulus-oocyte complexes (COC) or denuded oocytes (DO) were matured in tissue culture medium (TCM) supplemented with fetal bovine serum (FBS) or serum substitute supplement (SSS). Lipid profiles of TCM, serum supplements, immature CC and oocyte (IO), and in vitro-matured oocytes from COC and DO were then analyzed by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and submitted to partial least squares-discriminant analysis (PLS-DA). The developmental competence of such oocytes was also assessed. Differences in lipid composition were observed between two types of sera and distinctly influenced the lipid profile of CC. As revealed by PLS-DA, the abundance of specific ions corresponding to triacylglycerols (TAG) or phospholipids (PL) were higher in COC compared to DO both supplemented with FBS or SSS and to some extent affected the subsequent DO in vitro embryo development. DO exposed to SSS had however a marked diminished ability to develop to the blastocyst stage. These results indicate a modulation by CC of the oocyte TAG and PL profiles associated with a specific cell response to the serum supplement used for in vitro maturation.
authors.affiliation: a Department of Obstetrics and Gynecology, School of Medicine of Ribeirão Preto , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Alessandra A
authors.initials: AA
authors.lastname: Vireque
authors.affiliation: b ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry , University of Campinas , Campinas , SP , Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Tata
authors.affiliation: b ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry , University of Campinas , Campinas , SP , Brazil.
authors.forename: Katia Roberta A
authors.initials: KR
authors.lastname: Belaz
authors.affiliation: c Embrapa Dairy Cattle Research Center , Juiz de Fora , MG , Brazil.
authors.forename: João Gabriel V
authors.initials: JG
authors.lastname: Grázia
authors.affiliation: b ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry , University of Campinas , Campinas , SP , Brazil.
authors.forename: Fábio N
authors.initials: FN
authors.lastname: Santos
authors.affiliation: d In Vitro Brasil Ltda , Mogi Mirim , SP , Brazil.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Arnold
authors.affiliation: d In Vitro Brasil Ltda , Mogi Mirim , SP , Brazil.
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Basso
authors.affiliation: b ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry , University of Campinas , Campinas , SP , Brazil.
authors.forename: Marcos N
authors.initials: MN
authors.lastname: Eberlin
authors.affiliation: a Department of Obstetrics and Gynecology, School of Medicine of Ribeirão Preto , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Marcos Felipe
authors.initials: MF
authors.lastname: Silva-de-Sá
authors.affiliation: a Department of Obstetrics and Gynecology, School of Medicine of Ribeirão Preto , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Rui A
authors.initials: RA
authors.lastname: Ferriani
authors.affiliation: a Department of Obstetrics and Gynecology, School of Medicine of Ribeirão Preto , University of São Paulo , Ribeirão Preto , SP , Brazil.
authors.forename: Ana Carolina J
authors.initials: AC
authors.lastname: Sá Rosa-E-Silva
mesh_terms: D000818:Animals; D002417:Cattle; D002450:Cell Communication; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000378:metabolism*; D016002:Discriminant Analysis; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques; D016018:Least-Squares Analysis; D009865:Oocytes / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D010743:Phospholipids; D014280:Triglycerides
keywords: Fetal bovine serum; in vitro maturation; lipidomics; serum substitute supplement