title: MiR-9-5p Down-Regulates PiT2, but not PiT1 in Human Embryonic Kidney 293 Cells.
abstract: PiT1 (SLC20A1) and PiT2 (SLC20A2) are members of the mammalian type-III inorganic phosphate transporters and recent studies linked SLC20A2 mutations with primary brain calcifications. MicroRNAs (miRNAs) are endogenous noncoding regulatory RNAs and MicroRNA-9 (miR-9) modulates neurogenesis but is also involved with different types of cancer. We evaluated possible interactions between miR-9 and the phosphate transporters (PiT1 and PiT2). SLC20A2, platelet-derived growth factor receptor beta (PDGFRB) and Fibrillin-2 (FBN2) showed binding sites with high affinity for mir-9, In silico. miR-9 mimic was transfected into HEK293 cells and expression was confirmed by RT-qPCR. Overexpression of miR-9 in these cells caused a significant reduction in PiT2 and FBN2. PDGFRB appeared to be decreased, but was not significantly down-regulated. PiT1 showed no significant difference relative to controls. The down-regulation of PiT2 protein by miR-9 was confirmed by western blotting. In conclusion, we showed that miR-9 can down-regulate PiT2, in HEK293 cells. [corrected].
authors.affiliation: Keizo Asami Laboratory, Federal University of Pernambuco, Recife, PE, Brazil. darlenepaiva@gmail.com.
authors.forename: D P
authors.initials: DP
authors.lastname: Bezerra
authors.affiliation: Keizo Asami Laboratory, Federal University of Pernambuco, Recife, PE, Brazil. keasey@etsu.edu.|Department of Biomedical Sciences - Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA. keasey@etsu.edu.
authors.forename: M
authors.initials: M
authors.lastname: Keasey
authors.affiliation: Keizo Asami Laboratory, Federal University of Pernambuco, Recife, PE, Brazil. joao.ricardo@ufpe.br.|Neuropsychiatry Department, Federal University of Pernambuco, Recife, PE, Brazil. joao.ricardo@ufpe.br.
authors.forename: J R M
authors.initials: JRM
authors.lastname: Oliveira
mesh_terms: D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D050611:Sodium-Phosphate Cotransporter Proteins, Type III / Q000235:genetics* / Q000378:metabolism
chemical_list: C541874:MIRN92 microRNA, human; D035683:MicroRNAs; C493586:SLC20A1 protein, human; C493589:SLC20A2 protein, human; D050611:Sodium-Phosphate Cotransporter Proteins, Type III
keywords: HEK293 cells; PiT2; miR-9