title: Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression.
abstract: The thylakoid-associated kinases STN7 and STN8 are involved in short- and long-term acclimation of photosynthetic electron transport to changing light conditions. Here we report the identification of STN7/STN8 in vivo targets that connect photosynthetic electron transport with metabolism and gene expression. Comparative phosphoproteomics with the stn7 and stn8 single and double mutants identified two proteases, one RNA-binding protein, a ribosomal protein, the large subunit of Rubisco and a ferredoxin-NADP reductase as targets for the thylakoid-associated kinases. Phosphorylation of three of the above proteins can be partially complemented by STN8 in the stn7 single mutant, albeit at lower efficiency, while phosphorylation of the remaining three proteins strictly depends on STN7. The properties of the STN7-dependent phosphorylation site are similar to those of phosphorylated light-harvesting complex proteins entailing glycine or another small hydrophobic amino acid in the -1 position. Our analysis uncovers the STN7/STN8 kinases as mediators between photosynthetic electron transport, its immediate downstream sinks and long-term adaptation processes affecting metabolite accumulation and gene expression.
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle (Saale), Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Schönberg
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle (Saale), Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Rödiger
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle (Saale), Germany.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Mehwald
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle (Saale), Germany.
authors.forename: Johann
authors.initials: J
authors.lastname: Galonska
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle (Saale), Germany.
authors.forename: Gideon
authors.initials: G
authors.lastname: Christ
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle (Saale), Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Helm
authors.affiliation: Proteomeanalytik, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany.
authors.forename: Domenika
authors.initials: D
authors.lastname: Thieme
authors.affiliation: Proteomeanalytik, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Majovsky
authors.affiliation: Proteomeanalytik, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hoehenwarter
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Biozentrum, Weinbergweg 22, 06120, Halle (Saale), Germany.
authors.forename: Sacha
authors.initials: S
authors.lastname: Baginsky
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport / Q000235:genetics / Q000502:physiology*; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000235:genetics / Q000502:physiology; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C497212:STN7 protein, Arabidopsis; C504925:STN8 protein, Arabidopsis
keywords: STN7/STN8; chloroplast; long-term acclimation; phosphorylation targets; photosynthesis; state transitions