title: Long non-coding RNA HNF1A-AS1 promotes hepatocellular carcinoma cell proliferation by repressing NKD1 and P21 expression.
abstract: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Recent evidences have demonstrated that long non-coding RNAs (lncRNAs) act as key regulators of tumor development and progression including HCC. In the study, we showed that the expression level of HNF1A-AS1 was up-regulated in HCC cell lines. Furthermore, CCK-8 cell proliferation assays and cell cycle analysis showed that HNF1A-AS1 over-expression facilitated HCC cell proliferation by promoting the cell proliferation and S-phase progression, whereas HNF1A-AS1 knockdown had the opposite effect. Western-blotting analysis revealed that knockdown of HNF1A-AS1 inhibited the cycle-relative protein cyclin-D1 and PCNA expression in HCC cells. Mechanism, RNA immunoprecipitation (RIP) and Chromatin immunoprecipitation (ChIP) assays showed that by interacting with enhancer of zeste homolog 2 (EZH2), HNF1A-AS1 promoted HCC cell proliferation by repressing the NKD1 and p21 expression. These results suggested that HNF1A-AS1 may contribute to HCC progression, which may be an effective therapeutic target for patients.
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Affiliated Hospital of Qinghai University, Xining, 810001, China. Electronic address: wangcong112@21cn.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Affiliated Traditional Chinese Medicine Hospital Southwest Medical University, 182 Chunhui Road,Longmatan District,Luzhou,646000,China. Electronic address: moullin1978@21cn.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Mou
authors.affiliation: Department of Oncology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, PR China. Electronic address: chaihai12566@sina.com.
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Chai
authors.affiliation: Department of Gynecology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, PR China. Electronic address: wfwfwf12366@sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Imaging Department, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, PR China. Electronic address: 13971098006@163.com.
authors.forename: Yun-Zhi
authors.initials: YZ
authors.lastname: Yin
authors.affiliation: Division of Gastrointestinal Surgery, Department of General Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, Huai'an, PR China. Electronic address: zhangxiaoyu_ey@163.com.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008565:Membrane Proteins; D011485:Protein Binding; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C452880:Atp5md protein, rat; C493956:CDKN1A protein, human; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D008565:Membrane Proteins; C449091:NKD1 protein, human; D062085:RNA, Long Noncoding
keywords: Cell proliferation; HNF1A-AS1; Hepatocellular carcinoma; NKD1; P21