title: The influence of ozone and nutrition on δ13C in Betula pendula.
abstract: In the cellulose of stems and leaves, δ13C was investigated in a birch clone (Betula pendula), which was exposed throughout the growing season to either <3 (control) or 90/40 nl O31-1(day/night). Each regime was split into plants under high or low nutrient supply. δ13C was increased (becoming less negative), in stems rather than leaves, by both high nutrition (+2‰) and O3stress (+1‰). Whereas high nutrition raised the wateruse efficiency (WUE) while lowering the CO2concentration in the inner leaf air space (ci), WUE decreased and ciincreased under O3stress. Therefore, only the nutritional effect on the carbon isotope fractionation was reproduced by the model of Farquhar et al. (1982) which estimates WUE by means of δ13C based on ci. ciwas not biased by 'patchiness' in respect to stomatal opening. The latter was verified by microscopical analysis and the complete water infiltration of the birch leaves through the stomata, independent of the diurnal course of the leaf conductance for water vapour. Under low nutrient supply, the activity of phosphoenol pyruvate carboxylase (PEPC) was roughly doubled by ozone to about 1.3% of the total carboxylation capacity (by PEPC + rubisco), and was increased to 1.7% under high nutrition. The fractionation model, extended to account for varying activities of the carboxylating enzymes, indicated that stimulated PEPC was the cause of elevated δ13C, although ciwas increased under O3stress. The stimulation of PEPC and, as a consequence, elevated δ13C are discussed as part of a whole-plant acclimation to O3stress.
authors.affiliation: Paul Scherrer Institute, CH-5232, Villigen, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Saurer
authors.affiliation: Swiss Federal Institute for Forest, Snow and Landscape Research, Zürcherstrasee 111, CH-8903, Birmensdorf ZH, Switzerland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Maurer
authors.affiliation: Swiss Federal Institute for Forest, Snow and Landscape Research, Zürcherstrasee 111, CH-8903, Birmensdorf ZH, Switzerland.
authors.forename: Rainer
authors.initials: R
authors.lastname: Matyssek
authors.affiliation: Swiss Federal Institute for Forest, Snow and Landscape Research, Zürcherstrasee 111, CH-8903, Birmensdorf ZH, Switzerland.
authors.forename: Werner
authors.initials: W
authors.lastname: Landolt
authors.affiliation: Swiss Federal Institute for Forest, Snow and Landscape Research, Zürcherstrasee 111, CH-8903, Birmensdorf ZH, Switzerland.
authors.forename: Madeleine S
authors.initials: MS
authors.lastname: Günthardt-Goerg
authors.affiliation: Physics Institute, University of Berne, Sidlerstr. 5, CH-3012, Berne, Switzerland.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Siegenthaler
keywords: Betula pendula; Carboxylation; Nutrition; Ozone; δ13C