title: A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms.
abstract: A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymerase, which can be accomplished at a constant temperature. In step 2, the reaction product of step 1 is detected by a lateral flow biosensor, which is a rapid and cost effective tool for nuclei acid detection. Comparing with conventional methods, it requires no complicated machines. It is suitable for the use of point of care diagnostics. Therefore, this simple, cost effective, robust, and promising LFB detection method of SNP has great potential for the detection of genetic diseases, personalized medicine, cancer related mutations, and drug-resistant mutations of infectious agents.
authors.affiliation: Institute of Environment and Safety, Wuhan Academy of Agricultural Sciences, 1 Qinglingzhangjiawan, Hongshan District, Wuhan, China. zeng6@yahoo.com.
authors.forename: Lingwen
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Institute of Environment and Safety, Wuhan Academy of Agricultural Sciences, 1 Qinglingzhangjiawan, Hongshan District, Wuhan, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005838:Genotype; D006046:Gold; D018796:Immunoconjugates; D053768:Metal Nanoparticles; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D019095:Point-of-Care Systems; D020641:Polymorphism, Single Nucleotide
chemical_list: D018796:Immunoconjugates; D006046:Gold
keywords: Gold nanoparticle; Isothermal amplification; Lateral flow biosensor; Nucleic acid biosensor; Point-of-care test; SNP genotype