title: Cell adhesion pattern created by OSTE polymers.
abstract: Engineering surfaces with functional polymers is a crucial issue in the field of micro/nanofabrication and cell-material interface studies. For many applications of surface patterning, it does not need cells to attach on the whole surface. Herein, we introduce a novel polymer fabrication protocol of off-stoichiometry thiol-ene (OSTE) polymers to create heterogeneity on the surface by utilizing 3D printing and soft-lithography. By choosing two OSTE polymers with different functional groups, we create a pattern where only parts of the surface can facilitate cell adhesion. We also study the hydrophilic property of OSTE polymers by mixing poly(ethylene glycol) (PEG) directly with pre-polymers and plasma treatments afterwards. Moreover, we investigate the effect of functional groups' excess ratio and hydrophilic property on the cell adhesion ability of OSTE polymers. The results show that the cell adhesion ability of OSTE materials can be tuned within a wide range by the coupling effect of functional groups' excess ratio and hydrophilic property. Meanwhile, by mixing PEG with pre-polymers and undergoing oxygen plasma treatment afterward can significantly improve the hydrophilic property of OSTE polymers.
authors.affiliation: School of Biomedical Engineering, Institute for Personalized Medicine, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Liu
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Li
authors.forename: Xianting
authors.initials: X
authors.lastname: Ding
mesh_terms: D000072283:A549 Cells; D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D058626:Plasma Gases; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D066330:Printing, Three-Dimensional; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D058626:Plasma Gases; D011108:Polymers; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols