title: Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients.
abstract: Lepidium meyenii (Maca), originated from Peru, has been cultivated widely in China as a popular health care food. However, the chemical and effective studies of Maca were less in-depth, which restricted its application seriously. To ensure the quality of Maca, a feasible and accurate strategy was established. One hundred and sixty compounds including 30 reference standards were identified in 6 fractions of methanol extract of Maca by UHPLC-ESI-Orbitrap MS. Among them, 15 representative active compounds were simultaneously determined in 17 samples by UHPLC-ESI-QqQ MS. The results suggested that Maca from Yunnan province was the potential substitute for the one from Peru. Meanwhile, the neuroprotective effects of Maca were investigated. Three fractions and two pure compounds showed strong activities in the 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced zebrafish model. Among them, 80% methanol elution fraction (Fr5) showed significant neuroprotective activity, followed by 100% part (Fr6). The inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was a possible mechanism of its neuroprotective effect.
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Chinese Medical Sciences, University of Macau, Macau, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Pharmaceutical Sciences Pharmacognosy, University of Graz, Graz, Austria.
authors.forename: Adelheid
authors.initials: A
authors.lastname: Brantner
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: Shandong Rosemed Biopharm LTC, Yanzhou, Shandong province, China.
authors.forename: Xinbin
authors.initials: X
authors.lastname: Shu
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Si
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Baolin
authors.initials: B
authors.lastname: Bian
mesh_terms: D000818:Animals; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D029686:Lepidium / Q000737:chemistry*; D057230:Limit of Detection; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D015027:Zebrafish
chemical_list: D001688:Biological Products; D002800:Cholinesterase Inhibitors; D018696:Neuroprotective Agents