title: Development and validation of a UHPLC-MS/MS method for the simultaneous determination of five bioactive flavonoids in rat plasma and comparative pharmacokinetic study after oral administration of Xiaochaihu Tang and three compatibilities.
abstract: An accurate, rapid, and reliable ultra high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous determination of baicalin, wogonoside, baicalein, wogonin, and oroxylin A in rat plasma. Then, the stability of baicalin and baicalein in the preparation of plasma sample was systematically investigated. The Waters BEH C18column was used with a gradient mobile phase system of acetonitrile and water containing 0.1% formic acid. The analytes were detected in the multiple reaction monitoring mode with positive electrospray ionization. 100 μL fresh plasma was added with 50 μL antioxidant reagent (1 mol/L HCl containing 0.5% Vitamin C), and liquid-liquid extraction with ethyl acetate was used to extract the analytes from plasma. Lower limits of quantification of baicalin, wogonoside, baicalein, wogonin, and oroxylin A were 21.9, 4.80, 1.20, 0.848, and 0.800 ng/mL, respectively. The mean extract recoveries of five flavonoids were 69.1∼89.2%, and the precision and accuracy were within the acceptable limits. This method was further successfully applied to the comparative pharmacokinetic study of these five flavonoids in rats after oral administration of Xiaochaihutang and three compatibilities. The obtained results may be helpful to reveal the mechanism of Xiaochaihutang formula compatibility.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Bo
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.
authors.forename: Longshan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.
authors.forename: Chunfu
authors.initials: C
authors.lastname: Wu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C550200:xiaochaihu
keywords: comparative pharmacokinetics; flavonoids; tandem mass spectrometry; traditional Chinese medicine; ultra high performance liquid chromatography