title: Secreted phosphoprotein 24kD (Spp24) inhibits growth of hepatocellular carcinoma in vivo.
abstract: Several studies have shown that secreted phosphoprotein 24kD (Spp24) inhibits tumor growth. However, the effects of spp24 on hepatocellular carcinoma are not quite clear. In this study, we observed the inhibitory effect of spp24 on hepatocellular carcinoma in vivo. A subcutaneous hepatocellular carcinoma mice model was established by using Hep G2 cells. After sacrifice at day 40, tumor growth was assessed and tumor cell apoptosis and tumor cells proliferation were assessed by TUNEL assay and immunochemical analysis, respectively. BMP2 slightly stimulated the subcutaneous tumor growth compared with the control. Spp24 significantly inhibited the tumor growth and also abolished the BMP2-induced tumor growth (p<0.05). TUNEL assay and immunochemical analysis further showed that spp24 could enhance tumor cell apoptosis and inhibit cell proliferation (p<0.01). Our data show that spp24 can inhibit the growth of hepatocellular carcinoma. Spp24 may have great potential for cancer treatment.
authors.affiliation: Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Lao
authors.affiliation: Department of Orthopaedic Surgery, University of California at Los Angeles (UCLA), Los Angeles, CA, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Orthopaedic Surgery, University of California at Los Angeles (UCLA), Los Angeles, CA, USA.
authors.forename: Haijun
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address: renjispine@163.com.
authors.forename: Guibin
authors.initials: G
authors.lastname: Zhong
authors.affiliation: Geriatric Research, Education, and Clinical Center, VA Greater Los Angeles Health Care System, Sepulveda, CA, USA; Department of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Biomedical Engineering Interdepartmental Program, University of California, Los Angeles, Los Angeles, CA, USA.
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Murray
authors.affiliation: Department of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009368:Neoplasm Transplantation; D010750:Phosphoproteins / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D055396:Bone Morphogenetic Protein 2; D010750:Phosphoproteins; C091340:secreted phosphoprotein 24
keywords: Bone morphogenetic protein2; Hepatocellular carcinoma; Secreted phosphoprotein 24kD