title: Effect of Wnt Signaling on the Differentiation of Isletβ-Cells from Adipose-Derived Stem Cells.
abstract: The Wnt signaling is critical for pancreatic development and islet function; however, its precise effects on the development and function of theβ-cells remain controversial. Here we examined mRNA and protein expression of components of the Wnt signaling throughout the differentiation of isletβ-cells from adipose-derived stem cells (ADSCs). After induction, ADSCs expressed markers ofβ-cells, including theinsulin,PDX1, andglucagongenes, and the PDX1, CK19, nestin, insulin, and C-peptide proteins, indicating their successful differentiation. Compared with pancreatic adult stem cells (PASCs), the quantities ofinsulin,GLUT2, andIrs2mRNA decreased, whereasGcg,Gck,and Irs1mRNA increased. Over time, during differentiation,insulinmRNA and protein expression increased,GcgandGckmRNA expression increased,Irs1mRNA expression decreased and then increased, andIrs2mRNA increased and then decreased (allP< 0.05). The expression ofDvl-2,LRP5, andGSK3βmRNA as well as the Dvl-2, GSK3β, and p-GSK3βproteins also increased (P< 0.05). Expression ofTCF7L2(6-10 d) andβ-cateninmRNA as well as theβ-catenin protein increased but not significantly (P> 0.05). Our results indicate that the Wnt signaling is activated during ADSC differentiation into isletβ-cells, but there was no obvious enrichment of nonphosphorylatedβ-catenin protein.
authors.affiliation: National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China; Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China.
authors.forename: Hefei
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ren
authors.affiliation: National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ma
authors.affiliation: National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: National Research Center for Animal Transgenic Biotechnology, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China.
authors.forename: Dongjun
authors.identifier: 0000-0002-3201-0559
authors.initials: D
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000235:genetics; D051176:beta Catenin / Q000096:biosynthesis*
chemical_list: D007328:Insulin; D012333:RNA, Messenger; D051176:beta Catenin