title: Evidence against the participation of a pharmacokinetic interaction in the protective effect of single-dose curcumin against gastrointestinal damage induced by indomethacin in rats.
abstract: To determine the role of a pharmacokinetic interaction in the protective effect of curcumin against the gastric damage induced by indomethacin administration as such or as its prodrug acemetacin.
  
  
  
  Wistar rats orally received single dose of indomethacin (30 mg/kg) with and without curcumin (30 mg/kg); gastric injury was evaluated by determining the total damaged area. Additional groups of rats received an oral single dose of indomethacin (30 mg/kg) or its prodrug acemetacin (34.86 mg/kg) in the presence or absence of curcumin (30 mg/kg). Indomethacin and acemetacin concentrations in plasma from blood draws were determined by high-performance liquid chromatography.Plasma concentration-against-time curves were constructed, and bioavailability parameters, maximal concentration (Cmax) and area under the curve to the last sampling time (AUC0-t) were estimated.
  
  
  
  Concomitant administration of indomethacin and curcumin resulted in a significantly reduced gastric damage compared to indomethacin alone. However, co-administration of curcumin did not produce any significant alteration in the bioavailability parameters of indomethacin and acemetacin after administration of either the active compound or the prodrug.
  
  
  
  Curcumin exhibits a protective effect against indomethacin-induced gastric damage, but does not produce a reduction of the bioavailability of this nonsteroidal anti-inflammatory drug, indomethacin. Data thus suggest that a pharmacokinetic mechanism of action is not involved in curcumin gastroprotection.
authors.affiliation: Department of Pharmacology, Center for Research and Advanced Studies of the National Polytechnic Institute, 07360 Mexico City, Mexico.
authors.forename: Liliana
authors.initials: L
authors.lastname: Zazueta-Beltrán
authors.affiliation: Department of Pharmacology, Center for Research and Advanced Studies of the National Polytechnic Institute, 07360 Mexico City, Mexico.
authors.forename: Lorena
authors.initials: L
authors.lastname: Medina-Aymerich
authors.affiliation: Department of Pharmacology, Center for Research and Advanced Studies of the National Polytechnic Institute, 07360 Mexico City, Mexico.
authors.forename: Nadia
authors.initials: N
authors.lastname: Estela Díaz-Triste
authors.affiliation: Laboratory of Pharmacology, Institutional Program in Molecular Biomedicine, National School of Medicine and Homeopathy of the National Polytechnic Institute, Academic Area of Medicine of the Institute of Health Sciences, 07320 Mexico City, Mexico.
authors.forename: Aracely Evangelina
authors.initials: AE
authors.lastname: Chávez-Piña
authors.affiliation: Department of Pharmacology, Center for Research and Advanced Studies of the National Polytechnic Institute, 07360 Mexico City, Mexico.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Castañeda-Hernández
authors.affiliation: Superior Studies Faculty of Zaragoza, Universidad National Autonomous of Mexico, 09230 Mexico City, Mexico.
authors.forename: Leticia
authors.initials: L
authors.lastname: Cruz-Antonio
mesh_terms: D000818:Animals; D001682:Biological Availability; D003474:Curcumin / Q000494:pharmacology*; D004347:Drug Interactions; D007213:Indomethacin / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: C026784:acemetacin; D003474:Curcumin; D007213:Indomethacin