title: An expression vector inhibits gene expression in Xenopus embryos by antisense RNA.
abstract: An expression vector was constructed containing the entire bovine papilloma virus (BPV-1) genome and part of the a-actin gene of Xenopus laevis cloned in the antisense orientation into the neomycin resistance gene under the control of the herpes simplex virus (HSV) thymidine kinase (TK) promoter. When this vector is microinjected into X. laevis embryos it replicates extrachromosomally, at least up to the tadpole stage, and a fusion RNA is synthesized after the mid blastula transition (MBT). The expression of the antisense gene results in a morphological abnormality of somites demonstrating that antisense RNA generated by an episomal replicating expression vector can inhibit the expression of a selected gene during early embryogenesis of X. laevis.
authors.affiliation: Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, Ob dem Himmelreich 7, W-7400, Tübingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmid
authors.affiliation: Deutsches Krebsforschungszentrum, Institut für Experimentelle Pathologie, Heidelberg, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Steinbeisser
authors.affiliation: Institut für Anatomie und Zellbiologie, Universität Heidelberg, Heidelberg, Germany.
authors.forename: Hans-Henning
authors.initials: HH
authors.lastname: Epperlein
authors.affiliation: Deutsches Krebsforschungszentrum, Institut für Experimentelle Pathologie, Heidelberg, Germany.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Trendelenburg
authors.affiliation: Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, Ob dem Himmelreich 7, W-7400, Tübingen, Germany.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Lipps
keywords: Actin gene; Antisense RNA; BPV; Episomal expression vector