title: Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA.
abstract: Purpose. To compare the biocompatibility and antimicrobial effectiveness of the new Fast-Set MTA (FS-MTA) with ProRoot MTA (RS-MTA).Methods. The agar overlay method with neutral red dye was used. L929 mouse fibroblast cells were cultured. The liquid and oil extracts and solid test material were placed on the agar overlay, four samples for each material. Phenol was used as the positive control and cottonseed oil and MEM extracts were used as negative controls. Cytotoxicity was examined by measuring the zones of decolorization and evaluating cell lysis under an inverted microscope using the established criteria after 24 and 48 hours. The antimicrobial test was performed using the Kirby-Bauer disk-diffusion method againstS. mutans,E. faecalis,F. nucleatum,P. gingivalis, andP. intermedia. The size of the zone of inhibition was measured in millimeters.Results.There was no zone of decolorization seen under or around the test materials for FS-MTA and RS-MTA at 24 and 48 hours. The antimicrobial test demonstrated no inhibitory effect of FS-MTA or RS-MTA on any bacterial species after 24 and 48 hours.Conclusions.There was no cytotoxicity or bacterial inhibition observed by the new Fast-Set MTA when compared to the ProRoot MTA after setting.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Shin
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: Jung-Wei
authors.initials: JW
authors.lastname: Chen
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan.
authors.forename: Chi-Yang
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Biomechanics Research Laboratory, Center for Dental Research, Orthodontics and Dentofacial Orthopedics, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: Raydolfo
authors.initials: R
authors.lastname: Aprecio
authors.affiliation: Dental Education Services, School of Dentistry, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Yochim
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan; Division of Oral Rehabilitation and Center of Pediatric Dentistry, Department of Dentistry, Taipei Medical University Hospital, 252 Wu-Hsing Street, Taipei, Taiwan.
authors.forename: Naichia
authors.identifier: 0000-0003-0361-7404
authors.initials: N
authors.lastname: Teng
authors.affiliation: Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Torabinejad
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000472:pathogenicity; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D051379:Mice; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate