title: Bacterial magnetic particles improve testes-mediated transgene efficiency in mice.
abstract: Nano-scaled materials have been proved to be ideal DNA carriers for transgene. Bacterial magnetic particles (BMPs) help to reduce the toxicity of polyethylenimine (PEI), an efficient gene-transferring agent, and assist tissue transgene ex vivo. Here, the effectiveness of the BMP-PEI complex-conjugated foreign DNAs (BPDs) in promoting testes-mediated gene transfer (TMGT) in mouse was compared with that of liposome-conjugated foreign DNAs. The results proved that through testes injection, the clusters of BPDs successfully reached the cytoplasm and the nuclear of spermatogenesis cell, and expressed in testes of transgene founder mice. Additionally, the ratio of founder mice obtained from BPDs (88%) is about 3 times higher than the control (25%) (p < 0.05). Interestingly, the motility of sperms recovered from epididymis of the founder mice from BPD group were significantly improved, as compared with the control (p < 0.01). Based on classic breeding, the ratio of transgene mice within the first filial was significantly higher in BPDs compared with the control (73.8% versus 11.6%, p < 0.05). TMGT in this study did not produce visible histological changes in the testis. In conclusion, nano-scaled BPDs could be an alternative strategy for efficiently producing transgene mice in vivo.
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Sun
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Nana
authors.initials: N
authors.lastname: Kong
authors.affiliation: b School of Basic Medical Science, Capital Medical University , Beijing , China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Chi
authors.affiliation: b School of Basic Medical Science, Capital Medical University , Beijing , China.|c Department of Pathology , Eye Hospital of Hebei Province , Hebei , China , and.
authors.forename: Leilei
authors.initials: L
authors.lastname: Yang
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Xin
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Teng
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: d Ningxia Key Laboratory of Cerebrocranial Diseases, Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Ningxia Medical University , Yinchuan , China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Wen
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: a State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xia
mesh_terms: D000818:Animals; D018703:Founder Effect; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics*; D007097:Imines / Q000737:chemistry / Q000378:metabolism; D008081:Liposomes; D055944:Magnetosomes / Q000235:genetics* / Q000378:metabolism; D042201:Magnetospirillum / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D011095:Polyethylenes / Q000737:chemistry / Q000378:metabolism; D013081:Sperm Motility; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D019076:Transgenes
chemical_list: D007097:Imines; D008081:Liposomes; D011095:Polyethylenes; C505405:poly(ethylene imine); D049452:Green Fluorescent Proteins
keywords: Testis; bacterial magnetic particle; efficient; mice; transgene