title: Structural characters and protecting β-cells of a polysaccharide from flowers of Inula japonica.
abstract: Our previous studies found that the crude polysaccharides (IJP) from flowers of Inula japonica exhibited significantly anti-diabetic activity in alloxan or MLD-STZ induced diabetic mice. In this study, we will trace an active polysaccharide from IJP and investigate its physico-chemical property and its protective mechanism on islet cell damage. The result showed that an active polysaccharide (IJP-B-1) was isolated from IJP, its molecular mass was 3.7×104Da. IJP-B-1 was composed of rhamnose, arabinose, xylose, mannose, glucose, galactose and galactocuronic acid. Its major backbone structure was (1→3, 6)-linked-galactose and other branched residues. IJP-B-1 could protect pancreatic cells against STZ impairment at 50μg/mL and scavenge OH and O2radicals to decrease reactive oxygen generation in islet-cells in vitro. These results suggested that IJP-B-1 might be useful for protecting β-cells and against oxidative stress as an anti-diabetic candidate drug in future.
authors.affiliation: The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China. Electronic address: zcz54dr@163.com.
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: monkey514@126.com.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Diao
authors.affiliation: Institute of Radiation and Medicine, Academy of Military Medical Science, Beijing 100850, China. Electronic address: wangcz2002@aliyun.com.
authors.forename: Changzhen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: quws2000@sina.com.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qu
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: xn_02080589@aliyun.com.
authors.forename: Xiunan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: majordan@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Ma
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: shanjunjie001@126.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Shan
authors.affiliation: Department of Gastroenterology, Chinese PLA Postgraduate Medical School, 100853, China. Electronic address: sungh301xhk@163.com.
authors.forename: Guohui
authors.initials: G
authors.lastname: Sun
mesh_terms: D000818:Animals; D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects*; D035264:Flowers / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects*; D038143:Inula / Q000737:chemistry*; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical
keywords: Inula japonica; Physico-chemical property; Polysaccharide; β-cell