title: Prediction of human dietary δ15N intake from standardised food records: validity and precision of single meal and 24-h diet data.
abstract: Natural stable isotope ratios (δ15N) of humans can be used for nutritional analyses and dietary reconstruction of modern and historic individuals and populations. Information about an individual's metabolic state can be obtained by comparison of tissue and dietary δ15N. Different methods have been used to estimate dietary δ15N in the past; however, the validity of such predictions has not been compared to experimental values. For a total of 56 meals and 21 samples of 24-h diets, predicted and experimental δ15N values were compared. The δ15N values were predicted from self-recorded food intake and compared with experimental δ15N values. Predicted and experimental δ15N values were in good agreement for meals and preparations (r = 0.89, p < .001) as well as for the 24-h diets (r = 0.76, p < .001). Dietary δ15N was mainly determined by the amount of fish, whereas the contribution of meat to dietary δ15N values was less pronounced. Prediction of human dietary δ15N values using standardised food records and representative δ15N data sets yields reliable data for dietary δ15N intake. A differentiated analysis of the primary protein sources is necessary when relating the proportion of animal-derived protein in the diet by δ15N analysis.
authors.affiliation: a Institute of Biochemistry , German Sport University Cologne , Cologne , Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Hülsemann
authors.affiliation: b Department of Nutrition and Health Sciences , University of Nebraska-Lincoln , Lincoln , NE , USA.
authors.forename: Karsten
authors.initials: K
authors.lastname: Koehler
authors.affiliation: c Department of Hygiene , Social and Environmental Medicine Ruhr-University Bochum , Bochum , Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Wittsiepe
authors.affiliation: c Department of Hygiene , Social and Environmental Medicine Ruhr-University Bochum , Bochum , Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wilhelm
authors.affiliation: d Research Institute of Child Nutrition (FKE) , University of Bonn , Dortmund , Germany.
authors.forename: Annett
authors.initials: A
authors.lastname: Hilbig
authors.affiliation: d Research Institute of Child Nutrition (FKE) , University of Bonn , Dortmund , Germany.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Kersting
authors.affiliation: e German Research Centre of Elite Sports , German Sport University Cologne , Cologne , Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Braun
authors.affiliation: a Institute of Biochemistry , German Sport University Cologne , Cologne , Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Flenker
authors.affiliation: a Institute of Biochemistry , German Sport University Cologne , Cologne , Germany.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Schänzer
mesh_terms: D003611:Dairy Products / Q000032:analysis; D004032:Diet; D004044:Dietary Proteins / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry; D005247:Feeding Behavior; D005504:Food Analysis / Q000379:methods*; D005858:Germany; D006801:Humans; D008460:Meat / Q000032:analysis; D009587:Nitrogen Isotopes / Q000032:analysis*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D014675:Vegetables / Q000737:chemistry
chemical_list: D004044:Dietary Proteins; D009587:Nitrogen Isotopes
keywords: Diet study; food; human; nitrogen-15; protein