title: Constructing Tissuelike Complex Structures Using Cell-Laden DNA Hydrogel Bricks.
abstract: Tissue engineering has long been a challenge because of the difficulty of addressing the requirements that such an engineered tissue must meet. In this paper, we developed a new "brick-to-wall" based on unique properties of DNA supramolecular hydrogels to fabricate three-dimensional (3D) tissuelike structures: different cell types are encapsulated in DNA hydrogel bricks which are then combined to build 3D structures. Signal responsiveness of cells through the DNA gels was evaluated and it was discovered that the gel permits cell migration in 3D. The results demonstrated that this technology is convenient, effective and reliable for cell manipulation, and we believe that it will benefit artificial tissue fabrication and future large-scale production.
authors.affiliation: Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University , Beijing 100084, China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University , Beijing 100084, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shao
authors.affiliation: School of Basic Medical Sciences, Lanzhou University , Lanzhou, Gansu 730000, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Ma
authors.affiliation: Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University , Beijing 100084, China.
authors.forename: Bini
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, University of Strathclyde , Glasgow G4 0NW, United Kingdom.
authors.forename: Alan
authors.initials: A
authors.lastname: Faulkner-Jones
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, University of Strathclyde , Glasgow G4 0NW, United Kingdom.
authors.forename: Wenmiao
authors.initials: W
authors.lastname: Shu
authors.affiliation: Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University , Beijing 100084, China.
authors.forename: Dongsheng
authors.identifier: http://orcid.org/0000-0002-2583-818X
authors.initials: D
authors.lastname: Liu
mesh_terms: D004247:DNA / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004247:DNA
keywords: DNA hydrogel; cell arrangements; cell migration; self-healing; supramolecular; tissue engineering