title: Optimizing non-invasive radiofrequency hyperthermia treatment for improving drug delivery in 4T1 mouse breast cancer model.
abstract: Interactions of high-frequency radio waves (RF) with biological tissues are currently being investigated as a therapeutic platform for non-invasive cancer hyperthermia therapy. RF delivers thermal energy into tissues, which increases intra-tumoral drug perfusion and blood-flow. Herein, we describe an optical-based method to optimize the short-term treatment schedules of drug and hyperthermia administration in a 4T1 breast cancer model via RF, with the aim of maximizing drug localization and homogenous distribution within the tumor microenvironment. This method, based on the analysis of fluorescent dyes localized into the tumor, is more time, cost and resource efficient, when compared to current analytical methods for tumor-targeting drug analysis such as HPLC and LC-MS. Alexa-Albumin 647 nm fluorphore was chosen as a surrogate for nab-paclitaxel based on its similar molecular weight and albumin driven pharmacokinetics. We found that RF hyperthermia induced a 30-40% increase in Alexa-Albumin into the tumor micro-environment 24 h after treatment when compared to non-heat treated mice. Additionally, we showed that the RF method of delivering hyperthermia to tumors was more localized and uniform across the tumor mass when compared to other methods of heating. Lastly, we provided insight into some of the factors that influence the delivery of RF hyperthermia to tumors.
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Ware
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.|Department Biophysics Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Krzykawska-Serda
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Chak-Shing Ho
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.|Interdepartmental program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Jared
authors.initials: J
authors.lastname: Newton
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Suki
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Law
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.
authors.forename: Lam
authors.initials: L
authors.lastname: Nguyen
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.
authors.forename: Vazrik
authors.initials: V
authors.lastname: Keshishian
authors.affiliation: Institute of Chemistry, University of Silesia, Katowice, Poland.|Rice University, Department of Chemistry, Houston, TX, USA.
authors.forename: Maciej
authors.initials: M
authors.lastname: Serda
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Taylor
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.|Rice University, Department of Mechanical Engineering and Materials Science, Houston, TX, USA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Curley
authors.affiliation: Baylor College of Medicine, Department of Surgery, Houston, TX, USA.|Rice University, Department of Chemistry, Houston, TX, USA.|University of Houston, Department of Biomedical Engineering, Houston, TX, USA.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Corr
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001943:Breast Neoplasms / Q000628:therapy*; D004195:Disease Models, Animal; D004358:Drug Therapy / Q000379:methods*; D006979:Hyperthermia, Induced / Q000379:methods*; D051379:Mice; D061848:Optical Imaging; D011846:Radio Waves; D013194:Staining and Labeling
chemical_list: D000970:Antineoplastic Agents