title: The ameliorative effects of vinpocetine on apoptosis and HSP-70 expression in testicular torsion in rats.
abstract: Vinpocetine is a potent antioxidant and free radical scavenger. We investigated the effects of vinpocetine on torsion/detorsion (T/D) induced testicular damage, HSP-70 expression and germ cell apoptosis in rats. Sixty Wistar albino adult male rats were divided into five groups of 12. The groups comprised a control group, a sham treated group, a T/D group, a vinpocetine treated group, and a T/D plus vinpocetine treated group. The left testis of each rat was subjected to unilateral torsion followed by detorsion after 2 h. Vinpocetine was administered intraperitoneally immediately and for 10 days following detorsion. At the end of the study, the rats were sacrificed and their testes removed and processed. HSP-70 expression, apoptosis and histopathological damage scores were determined for each group. Testicular T/D caused significant increases in apoptosis and HSP-70 expression, and a significant decrease in Johnsen's testicular biopsy scores and mean seminiferous tubule diameter. Vinpocetine ameliorated testicular histopathology and HSP-70 expression in the T/D + vinpocetine group. Consequently, vinpocetine may prevent testicular injury following testicular torsion owing to its antioxidant effects.
authors.affiliation: a Departments of Histology and Embryology.
authors.forename: M F
authors.initials: MF
authors.lastname: Sönmez
authors.affiliation: a Departments of Histology and Embryology.
authors.forename: Ş
authors.initials: Ş
authors.lastname: Ozdemir
authors.affiliation: b Pediatric Surgery, Faculty of Medicine , Erciyes University , Kayseri , Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Guzel
authors.affiliation: a Departments of Histology and Embryology.
authors.forename: E
authors.initials: E
authors.lastname: Kaymak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D005854:Germ Cells / Q000187:drug effects / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000188:drug therapy*; D013737:Testis / Q000187:drug effects*; D014748:Vinca Alkaloids / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D014748:Vinca Alkaloids; C013983:vinpocetine
keywords: HSP-70; antioxidant; apoptosis; rat; testicular torsion; vinpocetine