title: Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4+CD25highFoxp3+regulatory T-cells in psoriasis.
abstract: BACKGROUND
  CD39 and CD73 are two novel cell surface markers of CD25highFoxp3+regulatory T-cells (Tregs). Concordant expression of these two ectoenzymes not only discriminate Tregs from other cell populations, but also generates pericellular adenosine, which has been reported to suppress proliferation of activated T effector (Teff) cells. Because it is currently unclear whether human ectoenzymes (CD39/CD73) are involved in the impaired suppressive activity of Tregs in psoriasis, we examined the frequencies and phenotypes of CD39/CD73-expressing Tregs and related receptor adenosine receptor 2A (A2AR) in peripheral blood of patients with different types of psoriasis.
  
  
  METHODS
  Peripheral blood mononuclear cells (PMBC) were prepared from patients with three different types of psoriasis (psoriasis vulgaris, pustular psoriasis and erythrodermic psoriasis). CD4+cells were separated from PBMC by negative selection on midiMACS columns, and the frequencies and phenotypes of CD39 and CD73 expressing Tregs, and A2AR expressing Teff were all determined by flow cytometry analysis. Blood from healthy volunteers served as controls.
  
  
  RESULTS
  The expression of single CD73+Tregs was markedly reduced (approximately 50%) in psoriasis vulgaris, compared to normal controls. In pustular psoriasis, the mean numbers of CD39+Tregs and A2AR+Teff was significantly lower than in normal controls. Among three different types of psoriasis, CD39 expression was strikingly reduced in the blood Treg population of pustular psoriasis patients. Decreased CD73+Tregs levels were observed in psoriasis vulgaris compared to pustular psoriasis and erythrodermic psoriasis.
  
  
  CONCLUSIONS
  The differences in the expression of CD39-and CD73-Tregs may be a factor in the pathogenesis of psoriasis.
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai, China.|Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.|Department of Dermatology, Seirei Yokohama General Hospital, Yokohama, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Sugiyama
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai, China.
authors.forename: Kexiang
authors.initials: K
authors.lastname: Yan
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: McCormick
authors.affiliation: Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.|The Murdough Family Center for Psoriasis, University Hospital Case Medical Center, Cleveland, Ohio, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Cooper
authors.affiliation: Department of Dermatology, Huashan Hospital, Shanghai, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Huang
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D018791:CD4 Lymphocyte Count; D016022:Case-Control Studies; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011565:Psoriasis / Q000097:blood / Q000276:immunology*; D043683:Receptors, Adenosine A2 / Q000097:blood*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D058851:GPI-Linked Proteins; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D043683:Receptors, Adenosine A2; D015720:5'-Nucleotidase; C548241:NT5E protein, human; D001081:Apyrase; C067869:CD39 antigen
keywords: A2AR; CD39; CD73; Tregs; ectoenzymes; psoriasis