title: A study of germinal mosaicism inDrosophila melanogaster.
abstract: Three-hundred and twenty fertile,pal-induced Y-chromosome mosaic males and females were obtained. Fractional analysis of the sons of 55 somatically mosaic flies that were also germinally mosaic tentatively suggests that the number of functional primordial germ cells inDrosophila melanogaster is variable and that it is seldom greater than 24. From the observed 0.17 frequency of germinal mosaicism it was estimated that the average number of pole cells at the end of blastoderm formation is 45. At present, the germ cells afford the only opportunity to compare genetic estimates of the number of blastoderm or primordial cells with available histological counts. The good agreement between them suggests that both the fractional and the mosaic frequency methods for estimating primordial or blastoderm cell numbers of various larval and imaginal anatomical structures provide reasonably close approximations of the actual values.
authors.affiliation: Department of Genetics, University of Wisconsin, 53706, Madison, Wisconsin, USA.
authors.forename: Moti
authors.initials: M
authors.lastname: Nissani
keywords: Drosophila; Germinal mosaicism; Number of primordial germ cells