title: Antithrombotic Effect and Mechanism of Radix Paeoniae Rubra.
abstract: The compounds of Radix Paeoniae Rubra (RPR) were isolated and identified by bioassay-guided method, and antithrombotic effects and mechanism were investigated by the acute blood stasis rat model. The RPR extract was evaluated by APTT, TT, PT, and FIB assays in vitro. Results indicated that RPR extract exhibited the anticoagulant activity. In order to find active compounds, six compounds were isolated and identified, and four compounds, paeoniflorin (Pae), pentagalloylglucose (Pen), albiflorin (Ali), and protocatechuic acid (Pro), exhibited the anticoagulant activity in vitro. Therefore, the antithrombosis effects of RPR extract and four active compounds were investigated in vivo by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of TXB2, 6-Keto-PGF1α, eNOS, and ET-1 were detected. Results suggested that RPR extract and four active compounds had the inhibition effect on thrombus formation, and the antithrombotic effects were associated with the regulation of vascular endothelium active substance, activating blood flow and anticoagulation effect.
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China.
authors.forename: Pingyao
authors.initials: P
authors.lastname: Xie
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Cui
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shan
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China.
authors.forename: Wen-Yi
authors.identifier: 0000-0002-1822-6249
authors.initials: WY
authors.lastname: Kang
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000737:chemistry; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000627:therapeutic use; D001681:Biological Assay; D001952:Bridged-Ring Compounds / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D019332:Endothelin-1 / Q000737:chemistry; D005260:Female; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000627:therapeutic use*; D005960:Glucosides / Q000737:chemistry; D047348:Hydrolyzable Tannins / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry; D008297:Male; D039821:Monoterpenes / Q000737:chemistry; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D029596:Paeonia / Q000737:chemistry*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000188:drug therapy*; D013929:Thromboxane B2 / Q000737:chemistry; D014783:Viscosity; D006027:Glycosides
chemical_list: D015121:6-Ketoprostaglandin F1 alpha; D000925:Anticoagulants; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D005343:Fibrinolytic Agents; D005960:Glucosides; D047348:Hydrolyzable Tannins; D062385:Hydroxybenzoates; D039821:Monoterpenes; D052250:Nitric Oxide Synthase Type III; D013929:Thromboxane B2; C015423:peoniflorin; D006027:Glycosides; C009091:protocatechuic acid; C014959:albiflorin; C435084:pentagalloylglucose; C496316:Nos3 protein, rat