title: A web-based microsatellite database for the Magnaporthe oryzae genome.
abstract: Microsatellites have been widely utilized for molecular marker development. Codominant and multiallelic nature of these simple repeats have several advantages over other types of molecular markers. Their broad applicability in the area of molecular biology like gene mapping, genome characterization, genome evolution, and gene regulation has been reported in various crop plants, animals and fungi. Considering these benefits of the SSR markers, a MMDB (Magnaporthe oryzae Microsatellite Database) was developed to help in understanding about the pathogen and its diversity at strains level of a particular geographic region, which can help us to make a proper utilization of blast resistance genes in the region. This microsatellite database is based on whole genome sequence of two M. oryzae isolates, RML-29 (2665 SSRs from 43037792 bp) and RP-2421 (3169 SSRs from 45510614 bp). Although, first M. oryzae genome (70-15) was sequenced in 2005, but this sequenced isolate is not a true field isolate of M. oryzae. Therefore, MMDB has great potential in the study of diversification and characterization of M. oryzae and other related fungi.
  
  
  BACKGROUND
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authors.affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi 110012, India; Department of Bioscience and Biotechnology, Banasthali University, Tonk, Rajasthan 304 022, India.
authors.forename: Pankaj Kumar
authors.initials: PK
authors.lastname: Singh
authors.affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi 110012, India.
authors.forename: Akshay
authors.initials: A
authors.lastname: Singh
authors.affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi 110012, India.
authors.forename: Deepak V
authors.initials: DV
authors.lastname: Pawar
authors.affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi 110012, India.
authors.forename: B N
authors.initials: BN
authors.lastname: Devanna
authors.affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi 110012, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Singh
authors.affiliation: Department of Bioscience and Biotechnology, Banasthali University, Tonk, Rajasthan 304 022, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Sharma
authors.affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi 110012, India.
authors.forename: Tilak R
authors.initials: TR
authors.lastname: Sharma
keywords: M. oryzae; MMDB; SSRs; rice blast