title: Anti-ListeriaActivity of Lactic Acid Bacteria in Two Traditional Sicilian Cheeses.
abstract: Listeria monocytogenesis a pathogen frequently found in dairy products, and its growth is difficult to control. Bacteriocin-like inhibitory substances (BLIS), produced by lactic acid bacteria (LAB), having provenin vitroanti-Listeriaactivity, could provide an innovative approach to controlL. monocytogenes; however, this application needs to be evaluatedin vivo. In this study, twenty LAB strains isolated from different Sicilian dairy environments were tested for control of growth ofL. monocytogenesin three different experimental trials. First, raw and UHT milk were inoculated with LAB strains alone, and LAB strains mixed withL. monocytogenes. Second, mini-cheeses containing LAB and/orL. monocytogeneswere produced. Third, two traditional Sicilian cheeses inoculated with a multi-strain LAB mixture combined withL. monocytogeneswere produced. The addition of BLIS produced by LAB to milk and in mini-cheese production was unable to inhibit the growth ofL. monocytogenes. However, an anti-Listeriaeffect was observed in thePecorino Sicilianocheeses, where, after 15 days of ripening, the cheeses with added LAB had fewerL. monocytogenescompared to the control cheeses with no added LAB, while in theVastedda della valle del Belìcecheeses, the multi-strain LAB mixture completely prevented the growth ofL. monocytogenes.
authors.affiliation: Institute for Experimental Veterinary Medicine of Sicily , Palermo, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Scatassa
authors.affiliation: Institute for Experimental Veterinary Medicine of Sicily, Palermo, Italy; Department of Agricultural and Forest Sciences, University of Palermo, Palermo, Italy.
authors.forename: Raimondo
authors.initials: R
authors.lastname: Gaglio
authors.affiliation: Institute for Experimental Veterinary Medicine of Sicily , Palermo, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Cardamone
authors.affiliation: Institute for Experimental Veterinary Medicine of Sicily , Palermo, Italy.
authors.forename: Giusi
authors.initials: G
authors.lastname: Macaluso
authors.affiliation: Local Health Unit , Palermo, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Arcuri
authors.affiliation: Department of Agricultural and Forest Sciences, University of Palermo , Palermo, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Todaro
authors.affiliation: Institute for Experimental Veterinary Medicine of Sicily , Palermo, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Mancuso
keywords: BLIS; In vivo applications; Listeria monocytogenes; Milk; Traditional Sicilian cheeses