title: Time-Lapse Imaging to Examine the Growth Kinetics of Arabidopsis Seedlings in Response to Ethylene.
abstract: Ethylene is well known to inhibit the growth of dark-grown eudicot seedlings. Most studies examine this inhibition after several days of exposure to ethylene. However, such end-point analysis misses transient responses and the dynamic nature of growth regulation. Here, high-resolution, time-lapse imaging is described as a method to gather data about ethylene growth kinetics and movement responses of the hypocotyls of dark-grown seedlings of Arabidopsis thaliana. These methods allow for the characterization of short-term kinetic responses and can be modified for the analysis of roots and seedlings from other species.
authors.affiliation: Department of Biochemistry & Cellular and Molecular Biology, M407 Walters Life Sciences, University of Tennessee, 1414 Cumberland Avenue, Knoxville, TN, 37996, USA. bbinder@utk.edu.
authors.forename: Brad M
authors.initials: BM
authors.lastname: Binder
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000254:growth & development*; D005030:Ethylenes / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects* / Q000254:growth & development*; D059008:Time-Lapse Imaging / Q000295:instrumentation / Q000379:methods
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene
keywords: Arabidopsis; Etiolated seedling; Growth; Hypocotyl; Nutation; Root; Time-lapse