title: Isolation and characterization of diterpene glycosides from Siegesbeckia pubescens.
abstract: Five new diterpenoid glycosides, siegesides A-E (1-5), along with the known compound darutoside (6) were isolated and characterized from the ethanol extract of Siegesbeckia pubescens. The structural elucidation of the isolates was accomplished by extensive HRESIMS and NMR analysis. Compounds 1 and 2 are epimers of 6. All isolates were evaluated for their inhibition on the migration of MB-MDA-231 breast cancer cells induced by the chemokine epithelial growth factor, and compound 2 showed superior inhibitory activities in comparison with the positive control drug with IC50value of 0.27μM.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100191, People's Republic of China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100191, People's Republic of China.
authors.forename: Kehui
authors.initials: K
authors.lastname: Xie
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy and Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, People's Republic of China.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100191, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Academy of Pharmaceutical Sciences, Jinan 250101, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100191, People's Republic of China. Electronic address: drhzfu@sina.com.
authors.forename: Hongzheng
authors.initials: H
authors.lastname: Fu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008958:Models, Molecular; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D006027:Glycosides; D010936:Plant Extracts; C062909:darutoside
keywords: Chemokine epithelial growth factor; Diterpenoid glycosides; MB-MDA-231 breast cancer cells; Siegesbeckia pubescens