title: Regulation of Rho GTPase activity at the leading edge of migrating cells by p190RhoGAP.
abstract: Cell migration, a key feature of embryonic development, immunity, angiogenesis, and tumor metastasis, is based on the coordinated regulation of actin dynamics and integrin-mediated adhesion. Rho GTPases play a major role in this phenomenon by regulating the onset and maintenance of actin-based protruding structures at cell leading edges (i.e. lamellipodia and filopodia) and contractile structures (i.e., stress fibers) at their trailing edge. While spatio-temporal analysis demonstrated the tight regulation of Rho GTPases at the migration front during cell locomotion, little is known about how the main regulators of Rho GTPase activity, such as GAPs, GEFs and GDIs, play a role in this process. In this review, we focus on a major negative regulator of RhoA, p190RhoGAP-A and its close isoform p190RhoGAP-B, which are necessary for efficient cell migration. Recent studies, including our, demonstrated that p190RhoGAP-A localization and activity undergo a complex regulatory mechanism, accounting for the tight regulation of RhoA, but also other members of the Rho GTPase family, at the cell periphery.
authors.affiliation: a Institut National de la Santé et de la Recherche Médicale , Bordeaux , France.|b Université de Bordeaux, Unité Mixte de Recherche 1053 Bordeaux Research In Translational Oncology , Bordeaux , France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Bidaud-Meynard
authors.affiliation: a Institut National de la Santé et de la Recherche Médicale , Bordeaux , France.|b Université de Bordeaux, Unité Mixte de Recherche 1053 Bordeaux Research In Translational Oncology , Bordeaux , France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Binamé
authors.affiliation: a Institut National de la Santé et de la Recherche Médicale , Bordeaux , France.|b Université de Bordeaux, Unité Mixte de Recherche 1053 Bordeaux Research In Translational Oncology , Bordeaux , France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Lagrée
authors.affiliation: a Institut National de la Santé et de la Recherche Médicale , Bordeaux , France.|b Université de Bordeaux, Unité Mixte de Recherche 1053 Bordeaux Research In Translational Oncology , Bordeaux , France.
authors.forename: Violaine
authors.initials: V
authors.lastname: Moreau
mesh_terms: D000818:Animals; D002465:Cell Movement; D020690:GTPase-Activating Proteins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D020690:GTPase-Activating Proteins; D007527:Isoenzymes; C085379:rho GTPase-activating protein; D020741:rho GTP-Binding Proteins
keywords: GTPase activating proteins; Rho GTPases; cell migration; lamellipodia; p190RhoGAP