title: Flexible and powerful strategy for qualitative and quantitative analysis of valepotriates in Valeriana jatamansi Jones using high-performance liquid chromatography with linear ion trap Orbitrap mass spectrometry.
abstract: Valeriana jatamansi Jones is an important medicinal plant and its quality is closely related to its region of origin. In the current study, we utilized a flexible and powerful strategy for comprehensive evaluation of the quality diversity for 15 regions in China. The method was based on a hybrid linear ion trap-Orbitrap mass spectrometry platform. For structure characterization, fragmentation patterns were detected by analyzing a series of standard compounds using data dependent multistage mass spectrometry acquisition. A fragment ion database for valepotriates was established, and the acquired data were high throughput filtered by fragment ion search for compound identification. For quantitative purposes, we normalized the mass spectrometry data of 15 samples using SIEVE 2.0 and the differences in composition were analyzed using principal component analysis combined with hierarchical clustering analysis. The results identified a total of 92 compounds from Valeriana jatamansi Jones. Samples from Dali, Kunming, and Baoshan have better qualities and concentrations of the main active constituents. To verify our strategy, we compared the valtrate, acevaltrate, and baldrinal contents using high-performance liquid chromatography with diode array detector. We developed and validated a comprehensive qualitative and quantitative analytical method to achieve quality control of Valeriana jatamansi Jones.
authors.affiliation: Beijing Institute of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Chunguo
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xinqi
authors.initials: X
authors.lastname: Deng
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Ma
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Shi
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D039823:Iridoids / Q000032:analysis*; D013058:Mass Spectrometry; D010946:Plants, Medicinal / Q000737:chemistry; D014632:Valerian / Q000737:chemistry*
chemical_list: D039823:Iridoids; C009643:valtrates (combination)
keywords: Valeriana jatamansi Jones; fragment ion search; hierarchical clustering analysis; principal component analysis; valepotriates