title: Detection of Cytomegalovirus (CMV) Infection in Wheezing Infants by Urine DNA and Serum IgG Testing.
abstract: BACKGROUND The aim of this study was to investigate the involvement of CMV infection in wheezing infants and the association between CMV-DNA and immunoglobulins (Igs). MATERIAL AND METHODS A total of 243 wheezing infants and 3,000 parturients were enrolled in this study. The infants were randomly grouped to receive blood HCMV-DNA tests (n=46) or urine HCMV-DNA tests (n=197). Furthermore, all participants had serum CMV-specific IgM and IgG testing. Afterwards, 10 HCMV-IgG positive infants were randomly selected for simultaneous blood and urine HCMV-DNA tests, and 25 HCMV-IgG positive puerperants were randomly selected for urine HCMV-DNA tests. RESULTS The detection rate of urine HCMV-DNA was significantly higher than that of blood HCMV-DNA (67.5% vs. 13.0%, p<0.001). Fifteen (6.2%) and 190 (80.0%) infants showed positive CMV-specific IgM and IgG results (p<0.001), respectively. Among the 10 HCMV-IgG positive infants tested further, only two infants had positive HCMV-DNA blood tests, while all of the 10 infants had positive HCMV-DNA urine tests. However, HCMV-DNA was not detected in the urine of the 25 randomly selected parturients positive for HCMV-IgG. CONCLUSIONS CMV infection may be one of the causes of wheezing in infants; CMV infection can be detected by urine-HCMV-DNA and serum HCMV-IgG testing. Infants were more susceptible to CMV infection than parturients.
authors.affiliation: Department of Pediatrics, No.101 Hospital of the PLA, Wuxi, Jiangsu, China (mainland).
authors.forename: Zhao-Cheng
authors.initials: ZC
authors.lastname: Zeng
authors.affiliation: Department of Pediatrics, Wuxi No. 8 People's Hospital, Wuxi, Jiangsu, China (mainland).
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.affiliation: Department of Pediatrics, No.101 Hospital of the PLA, Wuxi, Jiangsu, China (mainland).
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Sun
authors.affiliation: Department of Pediatrics, No.101 Hospital of the PLA, Wuxi, Jiangsu, China (mainland).
authors.forename: Ming-Mei
authors.initials: MM
authors.lastname: Song
authors.affiliation: Department of Pediatrics, No.101 Hospital of the PLA, Wuxi, Jiangsu, China (mainland).
authors.forename: Xin-Ling
authors.initials: XL
authors.lastname: Jin
authors.affiliation: Department of Pediatrics, No.101 Hospital of the PLA, Wuxi, Jiangsu, China (mainland).
authors.forename: Shu-Ya
authors.initials: SY
authors.lastname: Jiang
authors.affiliation: Department of Pediatrics, No.101 Hospital of the PLA, Wuxi, Jiangsu, China (mainland).
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000097:blood* / Q000175:diagnosis / Q000276:immunology / Q000652:urine*; D004279:DNA, Viral / Q000097:blood / Q000652:urine*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D008297:Male; D012135:Respiratory Sounds / Q000209:etiology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M