title: Dipeptidyl peptidase IV is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in Aspergillus aculeatus.
abstract: We screened for factors involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes from approximately 12,000 Aspergillus aculeatus T-DNA insertion mutants harboring a transcriptional fusion between the FIII-avicelase gene (cbhI) promoter and the orotidine 5'-monophosphate decarboxylase gene. Analysis of 5-fluoroorodic acid (5-FOA) sensitivity, cellulose utilization, and cbhI expression of the mutants revealed that a mutant harboring T-DNA at the dipeptidyl peptidase IV (dppIV) locus had acquired 5-FOA resistance and was deficient in cellulose utilization and cbhI expression. The deletion of dppIV resulted in a significant reduction in the cellulose-responsive expression of both cbhI as well as genes controlled by XlnR-independent and XlnR-dependent signaling pathways at an early phase in A. aculeatus. In contrast, the dppIV deletion did not affect the xylose-responsive expression of genes under the control of XlnR. These results demonstrate that DppIV participates in cellulose-responsive induction in A. aculeatus.
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Tani
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Yuki
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Kunitake
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.
authors.forename: Jun-Ichi
authors.initials: JI
authors.lastname: Sumitani
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawaguchi
mesh_terms: D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D044602:Cellulases / Q000235:genetics* / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism* / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D016254:Mutagenesis, Insertional; D009963:Orotic Acid / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014994:Xylose / Q000378:metabolism / Q000494:pharmacology
chemical_list: D004269:DNA, Bacterial; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C036483:T-DNA; D009963:Orotic Acid; C001242:5-fluoroorotic acid; D002482:Cellulose; D014994:Xylose; D044602:Cellulases; D018819:Dipeptidyl Peptidase 4; D009964:Orotidine-5'-Phosphate Decarboxylase
keywords: T-DNA insertion; cellulase-related genes; dipeptidyl peptidase IV; gene regulation