title: Structural insights into the EthR-DNA interaction using native mass spectrometry.
abstract: EthR is a transcriptional repressor that increases Mycobacterium tuberculosis resistance to ethionamide. In this study, the EthR-DNA interaction has been investigated by native electrospray-ionization mass spectrometry for the first time. The results show that up to six subunits of EthR are able to bind to its operator.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK. ca26@cam.ac.uk.
authors.forename: Daniel
authors.initials: D
authors.lastname: Shiu-Hin Chan
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK. ca26@cam.ac.uk.
authors.forename: Wei-Guang
authors.identifier: http://orcid.org/0000-0002-1138-6581
authors.initials: WG
authors.lastname: Seetoh
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK. ca26@cam.ac.uk.
authors.forename: Brendan N
authors.identifier: http://orcid.org/0000-0001-9002-9808
authors.initials: BN
authors.lastname: McConnell
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK. ca26@cam.ac.uk.
authors.forename: Dijana
authors.initials: D
authors.lastname: Matak-Vinković
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.
authors.forename: Sherine E
authors.initials: SE
authors.lastname: Thomas
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.
authors.forename: Vitor
authors.identifier: http://orcid.org/0000-0002-2734-2444
authors.initials: V
authors.lastname: Mendes
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.
authors.forename: Michal
authors.initials: M
authors.lastname: Blaszczyk
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK. ca26@cam.ac.uk.
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Coyne
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.
authors.forename: Tom L
authors.initials: TL
authors.lastname: Blundell
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK. ca26@cam.ac.uk.
authors.forename: Chris
authors.identifier: http://orcid.org/0000-0001-9174-1987
authors.initials: C
authors.lastname: Abell
mesh_terms: D002151:Calorimetry; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D005000:Ethionamide / Q000737:chemistry / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization; D013816:Thermodynamics
chemical_list: D004269:DNA, Bacterial; D005000:Ethionamide