title: Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model.
abstract: Whole-organ regeneration has great potential for the replacement of dysfunctional organs through the reconstruction of a fully functional bioengineered organ using three-dimensional cell manipulation in vitro. Recently, many basic studies of whole-tooth replacement using three-dimensional cell manipulation have been conducted in a mouse model. Further evidence of the practical application to human medicine is required to demonstrate tooth restoration by reconstructing bioengineered tooth germ using a postnatal large-animal model. Herein, we demonstrate functional tooth restoration through the autologous transplantation of bioengineered tooth germ in a postnatal canine model. The bioengineered tooth, which was reconstructed using permanent tooth germ cells, erupted into the jawbone after autologous transplantation and achieved physiological function equivalent to that of a natural tooth. This study represents a substantial advancement in whole-organ replacement therapy through the transplantation of bioengineered organ germ as a practical model for future clinical regenerative medicine.
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.|Department of Molecular Biology and Biochemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Ono
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.|Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan.|RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Oshima
authors.affiliation: Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan.|RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan.|Organ Technologies Inc., Tokyo, 105-0001, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Sonoyama
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Emilio Satoshi
authors.initials: ES
authors.lastname: Hara
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Oida
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Shinkawa
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Nakajima
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.|Department of Fixed Prosthodontics, Osaka University Graduate School of Dentistry, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mine
authors.affiliation: Department of Orthodontics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Hayano
authors.affiliation: Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, 980-8575, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukumoto
authors.affiliation: Section of Oral Implantology and Regenerative Dental Medicine, Graduate School of Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-8549, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Kasugai
authors.affiliation: Section of Oral Pathology, Department of Oral Restitution, Graduate School of Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 113-8549, Japan.|Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yamaguchi
authors.affiliation: Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan.|RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan.|Organ Technologies Inc., Tokyo, 105-0001, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.
authors.forename: Takuo
authors.initials: T
authors.lastname: Kuboki
mesh_terms: D000818:Animals; D001698:Biomedical Engineering / Q000639:trends; D004285:Dogs; D006801:Humans; D009805:Odontogenesis / Q000502:physiology; D012038:Regeneration / Q000502:physiology; D044968:Regenerative Medicine; D013234:Stem Cells; D023822:Tissue Engineering; D014070:Tooth / Q000254:growth & development / Q000637:transplantation*; D014078:Tooth Eruption; D014083:Tooth Germ / Q000502:physiology / Q000637:transplantation*; D014090:Tooth Replantation; D014182:Transplantation, Autologous / Q000379:methods