title: Burkholderia tropicaas a Potential Microalgal Growth-Promoting Bacterium in the Biosorption of Mercury from Aqueous Solutions.
abstract: The use of microalgal biomass is an interesting technology for the removal of heavy metals from aqueous solutions owing to its high metal-binding capacity, but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research was to investigate the potential ofBurkholderia tropicaco-immobilized withChlorellasp. in polyurethane discs for the biosorption of Hg(II) from aqueous solutions and to evaluate the influence of different Hg(II) concentrations (0.041, 1.0, and 10 mg/l) and their exposure to different contact times corresponding to intervals of 1, 2, 4, 8, 16, and 32 h. As expected, microalgal bacterial biomass adhered and grew to form a biofilm on the support. The biosorption data followed pseudo-second-order kinetics, and the adsorption equilibrium was well described by either Langmuir or Freundlich adsorption isotherm, reaching equilibrium from 1 h. In both bacterial and microalgal immobilization systems in the coimmobilization ofChlorellasp. andB. tropicato different concentrations of Hg(II), the kinetics of biosorption of Hg(II) was significantly higher before 60 min of contact time. The highest percentage of biosorption of Hg(II) achieved in the co-immobilization system was 95% at pH 6.4, at 3.6 g of biosorbent, 30 ± 1°C, and a mercury concentration of 1 mg/l before 60 min of contact time. This study showed that co-immobilization withB. tropicahas synergistic effects on biosorption of Hg(II) ions and merits consideration in the design of future strategies for the removal of toxic metals.
authors.affiliation: Doctoral Program in Applied Sciences - Coastal Marine Systems, Faculty of Marine Sciences and Biological Resources, University of Antofagasta, Antofagasta 1270300, Chile.|Laboratory of Microbial Complexity and Functional Ecology and Centre for Biotechnology and Bioengineering, Antofagasta Institute, University of Antofagasta, Antofagasta 1270300, Chile.|Microalgae Biotechnology Research Group, Faculty of Basic Sciences, University of Atlantico, Barranquilla 081007, Colombia.
authors.forename: Ana
authors.initials: A
authors.lastname: ZÁrate
authors.affiliation: Microalgae Biotechnology Research Group, Faculty of Basic Sciences, University of Atlantico, Barranquilla 081007, Colombia.|Centro i~mar & Centre for Biotechnology and Bioengineering, University of Los Lagos, Puerto Montt 5480000, Chile.
authors.forename: July
authors.initials: J
authors.lastname: Florez
authors.affiliation: Microalgae Biotechnology Research Group, Faculty of Basic Sciences, University of Atlantico, Barranquilla 081007, Colombia.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Angulo
authors.affiliation: Immunology and Molecular Biology Research Group, University of AtlÁntico, Barranquilla 081007, Colombia.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Varela-Prieto
authors.affiliation: Faculty of Pharmaceutical Sciences, Campus of Zaragocilla, University of Cartagena, Cartagena 130015, Colombia.
authors.forename: Cherlys
authors.initials: C
authors.lastname: Infante
authors.affiliation: Faculty of Pharmaceutical Sciences, Campus of Zaragocilla, University of Cartagena, Cartagena 130015, Colombia.
authors.forename: Fredy
authors.initials: F
authors.lastname: Barrios
authors.affiliation: Faculty of Health Sciences, CIUL, Universuty Libre, Barranquilla 080003, Colombia.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Barraza
authors.affiliation: Department of Mathematics, Faculty of Engineering, University of Atacama, Copiapó 1530000, Chile.
authors.forename: D I
authors.initials: DI
authors.lastname: Gallardo
authors.affiliation: Doctoral Program in Applied Sciences - Coastal Marine Systems, Faculty of Marine Sciences and Biological Resources, University of Antofagasta, Antofagasta 1270300, Chile.|Sedimentology and Paleoenvironmental Research Laboratory, Institute of Natural Sciences A. von Humboldt, Faculty of Marine Sciences and Biological Resources, University of Antofagasta, Av. Angamos 601, Antofagasta 1270330, Chile.|Doctoral Program in Science-Conservation and Management of Natural Resources, University of Los Lagos, Puerto Montt 5480000, Chile.
authors.forename: Jorge
authors.initials: J
authors.lastname: Valdés
mesh_terms: D065966:Absorption, Physicochemical; D000327:Adsorption; D001673:Biodegradation, Environmental; D018533:Biomass; D019117:Burkholderia / Q000502:physiology*; D018914:Cells, Immobilized; D002708:Chlorella / Q000254:growth & development / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008628:Mercury / Q000737:chemistry*; D058086:Microalgae / Q000254:growth & development / Q000502:physiology*; D011140:Polyurethanes; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011140:Polyurethanes; D014874:Water Pollutants, Chemical; D008628:Mercury
keywords: Burkholderia tropica; Metal biosorption; beta regression model; co-immobilization; isotherm modelling