title: Mitigation Effect of Proanthocyanidin on Secondary Heart Injury in Rats Caused by Mechanical Trauma.
abstract: Multiple organ dysfunctional syndrome secondary to mechanical trauma (MT) has attracted considerable research attention. The heart is one of the most important organs of the body, and secondary cardiac insufficiency caused by MT seriously affects the quality of life. This study aims to investigate whether proanthocyanidin can alleviate myocardial injury and improve heart function in the process of MT leading to secondary cardiac insufficiency. Noble-Collip drum wasused to prepare MT model in rats. And myocardial apoptosis index was calculated after TUNEL staining. Ventricular intubation was employed to detect heart function. Changes in myocardial ultrastructure were observed using an electron microscope. ELISA was used to detect the content of TNF-α and reactive oxygen species generated from monocytes and cardiomyocytes. The changes in Ca2+concentration in cardiomyocyte were observed by confocal microscope. Compared with trauma group, the administration group had a decreased apoptosis index of cardiomyocytes, and increased ±dp/dtmax. Meanwhile, proanthocyanidin can inhibit monocytes' TNF-α production, and reduce plasma TNF-α concentration. Moreover, proanthocyanidin can attenuate the excessive oxidative stress reaction of cardiomyocyte, and inhibit calcium overload in cardiomyocytes. In conclusion, proanthocyanidin can effectively ease myocardial damage and improve cardiac function, through anti-inflammatory and antioxidant effects in secondary cardiac insufficiency caused by MT.
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Ma
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Cao
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Xintao
authors.initials: X
authors.lastname: Li
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Deqin
authors.initials: D
authors.lastname: Yu
authors.affiliation: Dalian Medical University, Department of Physiology, Dalian, 116044, China.
authors.forename: Shuzhuang
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D006335:Heart Injuries / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D006801:Humans; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D044945:Proanthocyanidins / Q000008:administration & dosage*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D044945:Proanthocyanidins; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C013221:proanthocyanidin