title: Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription-reverse transcription concerted method in patients with low rectal cancer.
abstract: OBJECTIVE
  Positive carcinoembryonic antigen (CEA) messenger RNA (mRNA) in peritoneal lavage is associated with poor prognosis in patients with colon cancer. However, there are no reports about rectal cancer. Therefore we aimed to evaluate the frequency of positive CEA mRNA in peritoneal lavage and the significance of CEA mRNA in patients with low rectal cancer.
  
  
  METHODS
  A total of 55 patients with low rectal cancer who received curative surgical resection were enrolled. CEA mRNA in peritoneal lavage was measured using the transcription-reverse transcription concerted method, a quantitative RNA amplification method. The correlation between CEA mRNA and overall and peritoneal recurrence-free survival was evaluated.
  
  
  RESULTS
  Among 55 patients, 6 (10.9%) had positive CEA mRNA in peritoneal lavage. Patients with positive CEA mRNA resulted in significantly higher recurrence rate than those with negative CEA mRNA (p=0.007). Similarly, the local recurrence rate was significantly higher in the positive CEA mRNA group than in the negative CEA mRNA group (p=0.0009). Lymph node metastasis and positive CEA mRNA were independent risk factors for overall and local recurrence.
  
  
  CONCLUSIONS
  Positive CEA mRNA in low rectal cancer is a factor that predisposes patients to a high risk for overall recurrence, especially for local recurrence.
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan. Electronic address: muronok-sur@h.u-tokyo.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Murono
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Ishihara
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kawai
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kaneko
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Otani
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yasuda
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishikawa
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Tomomichi
authors.initials: T
authors.lastname: Kiyomatsu
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Hata
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nozawa
authors.affiliation: Department of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Satoh
authors.affiliation: Department of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Kurihara
authors.affiliation: Department of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality / Q000628:therapy; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000378:metabolism*; D003131:Combined Modality Therapy; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000378:metabolism; D010533:Peritoneal Lavage; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D012004:Rectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality / Q000628:therapy; D048348:Reverse Transcription; D015996:Survival Rate
chemical_list: D002272:Carcinoembryonic Antigen; D012333:RNA, Messenger
keywords: carcinoembryonic antigen messenger RNA; low rectal cancer; micrometastasis; peritoneal lavage; transcription–reverse transcription concerted method