title: Nuclear-Shell Biopolymers Initiated by Telomere Elongation for Individual Cancer Cell Imaging and Drug Delivery.
abstract: Here, we propose a strategy for unique nuclear-shell biopolymers initiated by telomere elongation for telomerase activity detection and precise drug delivery to individual cancer cells. Telomerase-triggered DNA rolling-circle amplification (RCA) is used to assemble nuclear-shell biopolymers with signal molecules for selective cancer cell recognition and efficient drug delivery to targeted individual cells. This strategy not only should allow the creation of clustered 5-carboxyfluorescein (FAM)-fluorescence spots in response to human-telomerase activity in individual cancer cells but also could efficiently deliver drugs to reduce the undesired death of healthy cells. These findings offer new opportunities to improve the efficacy of cancer cell imaging and therapy.
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University , Linyi 276000, P. R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University , Jinan 250014, P. R. China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, P. R. of China.
authors.forename: Mengting
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University , Linyi 276000, P. R. China.
authors.forename: Shusheng
authors.identifier: 0000-0002-8416-8331
authors.initials: S
authors.lastname: Zhang
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D002470:Cell Survival; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000235:genetics; D016503:Drug Delivery Systems; D005260:Female; D005452:Fluoresceins / Q000737:chemistry*; D005453:Fluorescence; D006367:HeLa Cells; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000000981:diagnostic imaging*; D061986:MCF-7 Cells; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000737:chemistry / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000000981:diagnostic imaging*
chemical_list: D001704:Biopolymers; D004273:DNA, Neoplasm; D005452:Fluoresceins; D012822:Silicon Dioxide; C045132:4-carboxyfluorescein; D019098:Telomerase