title: Identification and Validation of Gene Expression Patterns in Cystitis Glandularis Patients and Controls.
abstract: Our aim was to investigate differences in gene expression in bladder tissues between cystitis glandularis (CG) patients and healthy controls. Subsequent RNA was isolated from urinary bladder samples from CG patients and healthy controls, followed by RNA sequencing analysis. There were 4263 differentially expressed genes in urinary bladder between CG patients and controls, and 8 genes were verified with real-time PCR, Western blot, and enzyme-linked immunosorbent assay (ELISA) analysis. Gene set enrichment analysis (GSEA) revealed that 25 signaling pathways were upregulated in CG patients, and 17 signaling pathways were found upregulated in healthy controls. The mRNA expression levels of the indicated genes, including CCND1, CCNA1, EGFR, AR, CX3CL1, CXCL6, and CXCL1, were significantly increased in urinary bladder from CG and bladder cancer (BC) patients compared with healthy controls, while TP53 was decreased. CX3CL1, CXCL6, and CXCL1 concentrations in peripheral blood from CG and BC patients were significantly increased compared with healthy controls. The protein expression levels of CCND1, EGFR, and AR were significantly increased in urinary bladder from CG and BC patients compared with healthy controls. In conclusion, the gene expression profile of CG patients has established a foundation to study the gene mechanism of CG and BC progression.
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Chen-Long
authors.initials: CL
authors.lastname: Chu
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Chen-Hui
authors.initials: CH
authors.lastname: Zhao
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Wang
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Zhang
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: An-Qing
authors.initials: AQ
authors.lastname: Yang
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Ma
authors.affiliation: 2 Department of Pathology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Cai-Feng
authors.initials: CF
authors.lastname: Lu
authors.affiliation: 2 Department of Pathology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Mei-Zhen
authors.initials: MZ
authors.lastname: Gu
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Ren-Jie
authors.initials: RJ
authors.lastname: Cui
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Xin
authors.affiliation: 1 Department of Urology, Ruijin Hospital Luwan Branch affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: 3 Department of Urology, Ruijin Hospital affiliated with the Shanghai Jiaotong University Medical School, Shanghai, PR China.
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Zhou
mesh_terms: D000328:Adult; D015415:Biomarkers; D016022:Case-Control Studies; D019295:Computational Biology / Q000379:methods; D003556:Cystitis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D015415:Biomarkers
keywords: bladder cancer; cystitis glandularis; gene expression; signaling pathway