title: Human very Small Embryonic-like Cells Support Vascular Maturation and Therapeutic Revascularization Induced by Endothelial Progenitor Cells.
abstract: Very small embryonic-like stem cells (VSELs) are major pluripotent stem cells defined as cells of small size being Lineage- negative, CD133-positive, and CD45-negative. We previously described that human bone marrow VSELs were able to differentiate into endothelial cells and promoted post-ischemic revascularization in mice with surgically induced critical limb ischemia. In the present work, we isolated bone marrow VSELs from patients with critical limb ischemia and studied their ability to support endothelial progenitor cells therapeutic capacity and revascularization potential. Sorted bone marrow VSELs cultured in angiogenic media were co-injected with endothelial progenitor cells and have been show to trigger post-ischemic revascularization in immunodeficient mice, and support vessel formation in vivo in Matrigel implants better than human bone marrow mesenchymal stem cells. In conclusion, VSELs are a potential new source of therapeutic cells that may give rise to cells of the endothelial and perivascular lineage in humans. VSELs are the first real vasculogenic stem cells able to differentiate in endothelial and perivascular lineage in human adult described from now. Thus, because VSELs presence have been proposed in adult tissues, we think that VSELs are CD45 negative stem cells able to give rise to vascular regeneration in human tissues and vessels.
authors.affiliation: National Cytometry Platform, Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.
authors.forename: Coralie L
authors.initials: CL
authors.lastname: Guerin
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Paris, France.|Inserm UMR-S1140, Paris, France.
authors.forename: Elisa
authors.initials: E
authors.lastname: Rossi
authors.affiliation: Cellular and Molecular Imaging Facility, Inserm US 25, CNRS UMS 3612, Faculty of Pharmacy of Paris, Paris Descartes University, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Saubamea
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Paris, France.|Inserm UMR-S1140, Paris, France.|Cell Therapy Department, AP-HP, Saint Louis Hospital, Paris, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Cras
authors.affiliation: Cellular and Molecular Imaging Facility, Inserm US 25, CNRS UMS 3612, Faculty of Pharmacy of Paris, Paris Descartes University, Paris, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Mignon
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Paris, France.|Inserm UMRS-970, Paris Centre de Recherche Cardiovasculaire, Paris, France.
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Silvestre
authors.affiliation: Université Paris Descartes, Sorbonne Paris Cité, Paris, France. david.smadja@aphp.fr.|Inserm UMR-S1140, Paris, France. david.smadja@aphp.fr.|Hematology Department, AP-HP, European Georges Pompidou Hospital, Paris, France. david.smadja@aphp.fr.
authors.forename: David M
authors.initials: DM
authors.lastname: Smadja
mesh_terms: D000818:Animals; D066026:Endothelial Progenitor Cells / Q000378:metabolism / Q000637:transplantation; D005260:Female; D064593:Heterografts; D006614:Hindlimb / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007511:Ischemia / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic
keywords: ECFC; Endothelial progenitor cells; Perivascular cells; VSEL; Vasculogenesis; Very small embryonic like cells