title: Glutathione peroxidase activity and expression levels are significantly increased in acute coronary syndromes.
abstract: High levels of the antioxidant enzyme, glutathione peroxidase (GPx), have been associated with improved outcomes following acute coronary syndromes (ACS), suggesting a protective role. How GPx levels are altered with coronary disease is not clearly established. This study examined GPx activity, protein, and mRNA levels in healthy controls, patients with stable coronary artery disease (CAD), and patients with ACS. We studied 20 individuals from each of the healthy control, stable CAD, and ACS groups. GPx activity and protein levels, along with oxidized low-density lipoprotein (oxLDL) were assayed in plasma. GPx mRNA levels from whole blood were quantified using real-time PCR. Levels of GPx activity in the plasma were higher in ACS (109±7.7 U/mL) compared with patients with stable CAD (95.2±16.4 U/mL, p<0.01) and healthy controls (87.6±8.3 U/mL, p<0.001). Plasma GPx protein levels were also elevated in ACS (21.6±9.5 µg/mL) compared with patients with stable CAD (16.5±2.8 µg/mL, p<0.05) and healthy controls (16.3±5.3 µg/mL, p<0.05). Levels ofGPX1,GPX3, andGPX4mRNA were significantly higher in the patients with ACS. Levels of oxLDL were also significantly higher in patients with ACS (61.9±22.2 U/L) than in patients with stable CAD (47.8±10.4 U/L, p<0.05) and healthy controls (48.9±11.9 U/L, p<0.05). Levels of oxLDL, GPx activity, protein, and mRNA are all significantly higher in patients with ACS compared with patients with stable CAD and healthy controls. These findings suggest that GPx may be upregulated in response to a change in oxidative stress during an ACS.
authors.affiliation: School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.|Wellington Cardiovascular Research Group, Wellington, New Zealand.
authors.forename: Ana
authors.initials: A
authors.lastname: Holley
authors.affiliation: School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
authors.forename: Janet
authors.initials: J
authors.lastname: Pitman
authors.affiliation: School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.|Wellington Cardiovascular Research Group, Wellington, New Zealand.
authors.forename: John
authors.initials: J
authors.lastname: Miller
authors.affiliation: School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.|Wellington Cardiovascular Research Group, Wellington, New Zealand.|Department of Cardiology, Wellington Hospital, Wellington, New Zealand.
authors.forename: Scott
authors.initials: S
authors.lastname: Harding
authors.affiliation: School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.|Wellington Cardiovascular Research Group, Wellington, New Zealand.|Department of Surgery and Anaesthesia, Otago University, Wellington, New Zealand.
authors.forename: Peter
authors.initials: P
authors.lastname: Larsen
mesh_terms: D054058:Acute Coronary Syndrome / Q000201:enzymology*; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein; C486484:GPX3 protein, human; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
keywords: Antioxidants; Clinical Research; Coronary Artery Disease