title: A new material for tissue engineered vagina reconstruction: Acellular porcine vagina matrix.
abstract: Acellular matrix materials have been widely used to repair various tissues and organs. According to the plastic principle, when a part of the body is lost, it should be replaced with a similar material. Therefore, the use of a homologous organ-specific acellular vaginal tissue in vagina reconstruction repair surgery may show good results. However, the acellular vagina matrix (AVM) form large vertebrates is difficult to isolate. In this study, we described a multistep method to prepare porcine AVM and evaluated the efficacy of acellularization. We also investigated the biomechanical properties, biological activity elements, and biocompatibility of the porcine AVM. We then used this material to reconstruct a rat vagina and performed further morphologic and functional analyses. Small intestinal submucosa (SIS), which is a commonly used acellular matrix material, was used in a control group. Histological examination, DNA content analysis, and agarose gel electrophoresis revealed that the decellularization procedure was effective. The AVM had acceptable biomechanical properties and sufficient growth factor production (VEGF, FGF, TGF-β1, and PDGF-BB) compared with that of the SIS. Subcutaneous transplantation in rats showed that the AVM had good biocompatibility. The tissue-engineered vagina using the AVM more resembled normal-appearing tissue than did that using SIS following morphologic and functional analyses. The AVM has great potential for application in vaginal reconstructive surgery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1949-1959, 2017.
authors.affiliation: Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
authors.forename: Jing-Kun
authors.initials: JK
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
authors.forename: Run-Xuan
authors.initials: RX
authors.lastname: Du
authors.affiliation: Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
authors.forename: Ming-Le
authors.initials: ML
authors.lastname: Zhang
authors.affiliation: School of Mechanical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, 050018, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
authors.forename: Xiang-Hua
authors.initials: XH
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei, 050000, China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Xu
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism; D051381:Rats; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014621:Vagina
keywords: acellular matrix materials; acellular vagina matrix; small intestinal submucosa; tissue engineering; vagina reconstruction