title: Traditional Chinese medicine Bushen-Jianpi-Huoxue decoction prevents diabetic osteoporosis in rats via Wnt and nuclear factor-kappa B signaling pathways.
abstract: OBJECTIVE
  The aim of this study was to evaluate the therapeutic effect of a traditional Chinese medicine (TCM), Bushen-Jianpi-Huoxue decoction (BJHD), on diabetic osteoporosis (DOP) and the action mechanisms likely mediated by nuclear factor-kappa B (NF-κB) and Wnt signaling pathways.
  
  
  METHODS
  Fifty-five male Wistar rats were used in this study; they were divided into normal control (n = 10) and established DOP model (n = 45) groups. The DOP model was induced using a combination high carbohydrate - high fat diet and intraperitoneal injections of streptozotocin (STZ). The successfully induced animals were randomized to the model, Western medicine, TCM and control groups. Levels of fasting blood glucose; insulin; serum Ca, P and alkaline phosphatase, and the femoral bone mineral density (BMD) were measured. Furthermore, messenger RNA (mRNA) levels of cytokines in the Wnt and NF-κB signaling pathways were measured using reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Thirty rats were successfully established as the DOP model (10/group). After treatment, the levels of fasting blood glucose, insulin resistance and alkaline phosphatase in the TCM group rats were lower, while P and BMD were higher than those in the model groups. The mRNA levels of cytokines in the Wnt signaling pathway were higher in the TCM group than those in the model group. Moreover, the expressions of factors in the NF-κB pathway were markedly lower in the TCM group than they were in the model group.
  
  
  CONCLUSIONS
  Bushen-Jianpi-Huoxue decoction relieved DOP by activating the Wnt signaling pathway while inhibiting NF-κB signaling.
authors.affiliation: Department of Orthopedics, Shouguang Traditional Chinese Medicine Hospital, Shouguang, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Shouguang Traditional Chinese Medicine Hospital, Shouguang, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Shouguang Traditional Chinese Medicine Hospital, Shouguang, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Healthcare, Affiliated Hospital of ShanDong University of TCM, Jinan, Shandong, China.
authors.forename: Zetao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Traditional Chinese Medicine, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liang
authors.affiliation: Department of Traditional Chinese Medicine, Taishan Coal Sanatorum, Taian, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Endocrinology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shandong Academy of Medical Sciences, Jinan, Shandong, China.
authors.forename: Yihang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D015519:Bone Density / Q000187:drug effects*; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D059305:Diet, High-Fat; D004040:Dietary Carbohydrates; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D060492:Low Density Lipoprotein Receptor-Related Protein-5 / Q000235:genetics / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010024:Osteoporosis / Q000097:blood / Q000000981:diagnostic imaging / Q000209:etiology / Q000517:prevention & control*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D013311:Streptozocin; D048029:TNF Receptor-Associated Factor 6 / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D050071:Bone Density Conservation Agents; D050659:Core Binding Factor Alpha 1 Subunit; C495268:Ctnnb1 protein, rat; D004040:Dietary Carbohydrates; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D007328:Insulin; D060492:Low Density Lipoprotein Receptor-Related Protein-5; C555484:Lrp5 protein, rat; D016328:NF-kappa B; C584440:NFATC1 protein, rat; D012333:RNA, Messenger; C493717:Runx2 protein, rat; D048029:TNF Receptor-Associated Factor 6; D014157:Transcription Factors; D051176:beta Catenin; D013311:Streptozocin
keywords: Bushen Jianpi Huoxue decoction; NF-kappa B signaling pathway; Wnt signaling pathway; bone metabolism; diabetic osteoporosis