title: Real-Time In Vivo Hepatotoxicity Monitoring through Chromophore-Conjugated Photon-Upconverting Nanoprobes.
abstract: Drug toxicity is a long-standing concern of modern medicine. A typical anti-pain/fever drug paracetamol often causes hepatotoxicity due to peroxynitrite ONOO-. Conventional blood tests fail to offer real-time unambiguous visualization of such hepatotoxicity in vivo. Here we report a luminescent approach to evaluate acute hepatotoxicity in vivo by chromophore-conjugated upconversion nanoparticles. Upon injection, these nanoprobes mainly accumulate in the liver and the luminescence of nanoparticles remains suppressed owing to energy transfer to the chromophore. ONOO-can readily bleach the chromophore and thus recover the luminescence, the presence of ONOO-in the liver leads to fast restoring of the near-infrared emission. Taking advantages of the high tissue-penetration capability of near-infrared excitation/emission, these nanoprobes achieve real-time monitoring of hepatotoxicity in living animals, thereby providing a convenient screening strategy for assessing hepatotoxicity of synthetic drugs.
authors.affiliation: State Key Laboratory of Natural Medicines, School of Basic Medical Sciences and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China.|Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A* STAR), 138667, Singapore, Singapore.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Peng
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A* STAR), 138667, Singapore, Singapore.
authors.forename: Animesh
authors.initials: A
authors.lastname: Samanta
authors.affiliation: Department of Chemistry, National University of Singapore, 117543, Singapore, Singapore.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Chemistry, National University of Singapore, 117543, Singapore, Singapore.|Department of Orthopedic Surgery, National University of Singapore, 119228, Singapore, Singapore.
authors.forename: Sanyang
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Chemistry, National University of Singapore, 117543, Singapore, Singapore.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A* STAR), 138667, Singapore, Singapore.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Su
authors.affiliation: Singapore Institute of Neurotechnology (SINAPSE), National University of Singapore, 117456, Singapore, Singapore.
authors.forename: Daniel Teh Boon
authors.initials: DT
authors.lastname: Loong
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A* STAR), 138667, Singapore, Singapore.
authors.forename: Nam-Young
authors.initials: NY
authors.lastname: Kang
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A* STAR), 138667, Singapore, Singapore.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Orthopedic Surgery, National University of Singapore, 119228, Singapore, Singapore.|Departments of Biomedical Engineering and Neurology, Johns Hopkins University, Baltimore, USA.
authors.forename: Angelo Homayoun
authors.initials: AH
authors.lastname: All
authors.affiliation: Department of Orthopedic Surgery, National University of Singapore, 119228, Singapore, Singapore.
authors.forename: Wenxuan
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, National University of Singapore, 117543, Singapore, Singapore.|State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of Chemistry, National University of Singapore, 117543, Singapore, Singapore.|Institute of Materials Research and Engineering, Agency for Science Technology and Research (A* STAR), 117602, Singapore, Singapore.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A* STAR), 138667, Singapore, Singapore.|Department of Chemistry, National University of Singapore, 117543, Singapore, Singapore.
authors.forename: Young-Tae
authors.identifier: 0000-0002-1927-3688
authors.initials: YT
authors.lastname: Chang
keywords: biosensors; fluorescent probes; hepatotoxicity; nanotechnology; paracetamol