title: CD4+memory T cells retain surface expression of CD31 independently of thymic function in patients with lymphoproliferative disorders following autologous hematopoietic stem-cell transplantation.
abstract: High-dose chemotherapy with autologous hematopoietic stem-cell transplantation (AHSCT) causes severe and long-lasting immunodeficiency in patients with lymphoproliferative disorders. The thymus begins to restore the T-cell repertoire approximately from the sixth month post-transplant. We assessed the dynamics of post-transplant recovery of CD4+CD45RA+CD31+T cells, "recent thymic emigrants" (RTEs), and a poorly described subtype of CD4+CD45RA-CD31+T cells in 90 patients with lymphoproliferative disorders following high-dose chemotherapy with AHSCT. Relative and absolute counts of CD4+CD31+naïve and memory T cells were evaluated before AHSCT, at the day of engraftment, and 6- and 12-month post-transplant. The pre-transplant count of CD4+CD45RA+CD31+T cells was lower than in healthy controls, and did not reach donors' values during the 12-month period. The pre-transplant number of CD4+CD45RA-CD31+T cells was higher than in healthy controls and was restored rapidly following AHSCT. Post-transplant mediastinal radiotherapy reduced counts of RTEs and elongated recovery period. Non-thymic tissue irradiation did not reduce this subset. The obtained data indicate that homeostatic proliferation may decrease the significance of CD31 expression on CD4+CD45RA+T cells as a marker of RTEs, and suggest that evaluation of RTEs recovery by flow cytometry requires an accurate gating strategy to exclude CD31+memory T cells.
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation. Ebatorov@gmail.com.
authors.forename: Egor V
authors.identifier: http://orcid.org/0000-0003-2902-9336
authors.initials: EV
authors.lastname: Batorov
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Marina A
authors.initials: MA
authors.lastname: Tikhonova
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Kryuchkova
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Vera V
authors.initials: VV
authors.lastname: Sergeevicheva
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Sizikova
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Galina Y
authors.initials: GY
authors.lastname: Ushakova
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Dariya S
authors.initials: DS
authors.lastname: Batorova
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Gilevich
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Ostanin
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Ekaterina Y
authors.initials: EY
authors.lastname: Shevela
authors.affiliation: Research Institute of Fundamental and Clinical Immunology, 14 Yadrintsevskaya St, 630099, Novosibirsk, Russian Federation.
authors.forename: Elena R
authors.initials: ER
authors.lastname: Chernykh
mesh_terms: D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D016022:Case-Control Studies; D002462:Cell Membrane / Q000378:metabolism; D003131:Combined Modality Therapy; D005260:Female; D015870:Gene Expression; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D018655:Lymphocyte Count; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D013934:Thymectomy; D013950:Thymus Gland / Q000276:immunology* / Q000601:surgery; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Autologous hematopoietic stem-cell transplantation; CD31; Immune reconstitution; Lymphoproliferative disorders; Recent thymic emigrants