title: Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and Other Prospective Peripheral Membrane Proteins.
abstract: Peripheral binding of proteins to lipid bilayers is critical not only in intracellular signaling but also in metalloproteinase shedding of signaling proteins from cell surfaces. Assessment of how proteins recognize fluid bilayers peripherally using crystallography or structure-based predictions has been important but incomplete. Assay of dynamic protein-bilayer interactions in solution has become feasible and reliable using paramagnetic NMR and site-directed fluor labeling. Details of preparations and assay protocols for these spectroscopic measurements of bilayer proximity or contact, respectively, are described.
authors.affiliation: Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO, 65211, USA.
authors.forename: Tara C
authors.initials: TC
authors.lastname: Marcink
authors.affiliation: Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO, 65211, USA.|Department of Medical Microbiology and Immunology, Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Rama K
authors.initials: RK
authors.lastname: Koppisetti
authors.affiliation: Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO, 65211, USA.
authors.forename: Yan G
authors.initials: YG
authors.lastname: Fulcher
authors.affiliation: Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO, 65211, USA. vandorens@missouri.edu.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Van Doren
mesh_terms: D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D045726:Metalloproteases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D012995:Solubility
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D045726:Metalloproteases
keywords: Environment-sensitive fluorophore; Metalloproteinase; NMR; Paramagnetic relaxation enhancement; Peripheral membrane protein; Protein–lipid interactions; Structure-based prediction