title: Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247.
abstract: Aneurinibacillus: sp. YR247 was newly isolated from the deep-sea sediment inside the Calyptogena community at a depth of 1171 m in Sagami Bay. The strain exhibited antifungal activity against the filamentous fungus Aspergillus brasiliensis NBRC9455. A crude extract prepared from the YR247 cells by ethanol extraction exhibited broad antimicrobial activities. The antifungal compound is stable at 4-70 °C and pH 2.0-12.0. After treatment with proteinase K, the antifungal activity was not detected, indicating that the antifungal compound of strain YR247 is a peptidic compound. Electrospray ionization mass spectrometry of the purified antifungal compound indicated that the peptidic compound has an average molecular weight of 1167.9. The molecular weight of the antifungal compound from strain YR247 is different from those of antimicrobial peptides produced by the related Aneurinibacillus and Bacillus bacteria. The antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247 may be useful as a biocontrol agent.
authors.affiliation: Faculty of Agriculture, Kindai University, 3327-204 Nakamachi, Nara City, Nara, 631-8505, Japan. kurata090401@nara.kindai.ac.jp.
authors.forename: Atsushi
authors.identifier: http://orcid.org/0000-0002-0235-0261
authors.initials: A
authors.lastname: Kurata
authors.affiliation: Faculty of Agriculture, Kindai University, 3327-204 Nakamachi, Nara City, Nara, 631-8505, Japan.
authors.forename: Yuto
authors.initials: Y
authors.lastname: Yamaura
authors.affiliation: Faculty of Agriculture, Kindai University, 3327-204 Nakamachi, Nara City, Nara, 631-8505, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka, 237-0061, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
authors.affiliation: Faculty of Engineering, Kindai University, 1 Takaya Umenobe, Higashi-Hiroshima City, Hiroshima, 739-2116, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Nakasone
authors.affiliation: Faculty of Agriculture, Kindai University, 3327-204 Nakamachi, Nara City, Nara, 631-8505, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kishimoto
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects; D056449:Bacillales / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D019015:Geologic Sediments / Q000382:microbiology; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010802:Phylogeny; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000935:Antifungal Agents; D010455:Peptides
keywords: Antifungal activity; Calyptogena community; Deep-sea bacterium Aneurinibacillus sp. YR247; Peptidic compound; Sagami Bay