title: Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus.
abstract: Understanding the human brain is the ultimate goal in neuroscience, but this is extremely challenging in part due to the fact that brain tissue obtained from autopsy is practically the only source of normal brain tissue and also since changes at different levels of biological organization (genetic, molecular, biochemical, anatomical) occur after death due to multiple mechanisms. Here we used metabolomic and anatomical techniques to study the possible relationship between post-mortem time (PT)-induced changes that may occur at both the metabolomics and anatomical levels in the same brains. Our experiments have mainly focused on the hippocampus of the mouse. We found significant metabolomic changes at 2 h PT, whereas the integrity of neurons and glia, at the anatomical/ neurochemical level, was not significantly altered during the first 5 h PT for the majority of histological markers.
authors.affiliation: CEMBIO (Centre for Metabolomics and Bioanalysis), Facultad de Farmacia, Universidad CEU San Pablo, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Gonzalez-Riano
authors.affiliation: Laboratorio Cajal de Circuitos Corticales (CTB), Universidad Politécnica de Madrid, Madrid, Spain.|Instituto Cajal (CSIC), Avenida Doctor Arce 37, 28002, Madrid, Spain.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Tapia-González
authors.affiliation: CEMBIO (Centre for Metabolomics and Bioanalysis), Facultad de Farmacia, Universidad CEU San Pablo, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: García
authors.affiliation: Laboratorio Cajal de Circuitos Corticales (CTB), Universidad Politécnica de Madrid, Madrid, Spain.|Instituto Cajal (CSIC), Avenida Doctor Arce 37, 28002, Madrid, Spain.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, Spain.|Department of Cell Biology, Complutense University, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Muñoz
authors.affiliation: Laboratorio Cajal de Circuitos Corticales (CTB), Universidad Politécnica de Madrid, Madrid, Spain. defelipe@cajal.csic.es.|Instituto Cajal (CSIC), Avenida Doctor Arce 37, 28002, Madrid, Spain. defelipe@cajal.csic.es.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, Spain. defelipe@cajal.csic.es.
authors.forename: Javier
authors.initials: J
authors.lastname: DeFelipe
authors.affiliation: CEMBIO (Centre for Metabolomics and Bioanalysis), Facultad de Farmacia, Universidad CEU San Pablo, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain. cbarbas@ceu.es.
authors.forename: Coral
authors.initials: C
authors.lastname: Barbas
mesh_terms: D000818:Animals; D001344:Autopsy; D015415:Biomarkers / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008810:Mice, Inbred C57BL; D009445:Neuroanatomy / Q000379:methods*; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D011180:Postmortem Changes; D013997:Time Factors
chemical_list: D015415:Biomarkers
keywords: 2-Arachidonoylglycerol; anandamide; Arginine; Aspartic acid; Astrocytes; Brain tissue; Cadaverine; Calbindin-D28K (CB); Creatine; GABA; GABAergic interneurons; GAD-65; GC–MS analysis; Gangliosides; Glutamate; Glycerophosphocholine; Glycerophospholipids; Hippocampus; Histology; LC–MS analysis; Metabolites; Metabolomic changes; Microglia; NADPH-diaphorase (NADPH-d) glutamatergic axon terminals; Neuronal markers; Parvalbumin; Phosphatidylcholine and phosphatidylethanolamine; Post-mortem delay; Putrescine; SMI-32