title: Diospyros lotus leaf and grapefruit stem extract synergistically ameliorate atopic dermatitis-like skin lesion in mice by suppressing infiltration of mast cells in skin lesions.
abstract: Atopic dermatitis, a chronic relapsing and pruritic inflammation of the skin also thought to be involved in, or caused by immune system destruction is an upsetting health problem due to its continuously increasing incidence especially in developed countries. Mast cell infiltration in atopic dermatitis skin lesions and its IgE-mediated activation releases various cytokines and chemokines that have been implicated in the pathogenesis of atopic dermatitis. This study was aimed at investigating synergistic anti-inflammatory, anti-pruritic and anti-atopic dermatitis effects of Diospyros lotus leaf extract (DLE) and Muscat bailey A grapefruit stem extract (GFSE) in atopic dermatitis-like induced skin lesions in mice. Combinations of DLE and GFSE inhibited TNF-α and IL-6 production more than DLE or GFSE in PMA plus calcium ionophore A23187-activated HMC-1 cells. DLE and GFSE synergistically inhibited compound 48/80-induced dermal infiltration of mast cells and reduced scratching behavior than DLE or GFSE. Furthermore, DLE and GFSE synergistically showed a stronger ameliorative effect in skin lesions by reducing clinical scores; dermal infiltration of mast cells; ear and dorsal skin thickness; serum IgE and IL-4 production in atopic dermatitis-like mice. Collectively, these results suggest that DLE and GFSE synergistically exhibit anti-atopic dermatitis effects in atopic dermatitis-like skin lesions in mice.
authors.affiliation: Research Institute, Ato Q&A Corporation, Jeonju 55069, Republic of Korea; Department of Health Care & Science, Jeonju University, Jeonju 55069, Republic of Korea.
authors.forename: Byoung Ok
authors.initials: BO
authors.lastname: Cho
authors.affiliation: Department of Health Care & Science, Jeonju University, Jeonju 55069, Republic of Korea.
authors.forename: Denis Nchang
authors.initials: DN
authors.lastname: Che
authors.affiliation: Research Institute, Ato Q&A Corporation, Jeonju 55069, Republic of Korea.
authors.forename: Hong Hua
authors.initials: HH
authors.lastname: Yin
authors.affiliation: Department of Health Care & Science, Jeonju University, Jeonju 55069, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Research Institute, Ato Q&A Corporation, Jeonju 55069, Republic of Korea; Department of Health Care & Science, Jeonju University, Jeonju 55069, Republic of Korea; Agri-Bio Institute, Jeonju University, Jeonju 55069, Republic of Korea. Electronic address: sonjjang@jj.ac.kr.
authors.forename: Seon Il
authors.initials: SI
authors.lastname: Jang
mesh_terms: D000818:Animals; D032083:Citrus paradisi / Q000737:chemistry*; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000473:pathology; D029788:Diospyros / Q000737:chemistry*; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*
chemical_list: D010936:Plant Extracts
keywords: Atopic dermatitis; Diospyros lotus leaf; Grapefruit stem; Mast cell; Scratching behavior