title: Immunohistochemical detection of subunit A(M) of lactate dehydrogenase in tissues of the developing chick.
abstract: The distribution of A(M) subunits of lactate dehydrogenase (mainly LDH5) in developing muscle, heart, liver, lung, kidney and cartilage tissue of chicken embryos was examined by the indirect fluorescent antibody technique. Antibodies against porcine LDH5, purified by affinity chromatography, were used for this purpose. In special areas of newly formed myofibrils in somitic myoblasts fluorescence was already detected after 4 days of incubation, and located at the same place in muscle tissue of all advanced developmental stages examined. During the myotube stage of muscle development staining was also located in the peripheral thickened cytoplasma of the myotubes. The myocardium did not exhibit any fluorescent staining in the developmental stages examined. Endocardium, epicardium and pericardium, however, were fluorescent in young developmental stages. The liver showed fluorescence in 5- to 8-day embryos mainly in the endothelial cells of the blood sinusoids. In 9- to 12-day embryos the bile ducts became fluorescent. In lungs after 9- to 12-day development the epithelium and the surrounding tissues of bronchi exhibited strong immunofluorescence. The mesonephros exhibited faint granular fluorescence in tubule-forming cells and their membranes after 4-9 days of incubation. Advanced developmental stages only exhibited fluorescent blood cells. This latter staining is at least partly due to non-specific reactions of blood cell membranes with FITC-conjugated anti-rabbit IgG. Cartilage is characterized by non-specific fluorescence, but in embryos older than 8 days strong granular fluorescence of chondrocytes and staining of the perichondrium distinguished sections treated with anti-LDH5antibodies from control sections reacted only with FITC-conjugated anti-rabbit IgG. In addition, strong fluorescent staining was detectable in certain areas of the 5-day neural tube and faint staining in the mucosa of the intestine from embryos older than 10 days.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Ilse
authors.initials: I
authors.lastname: Faulhauber
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Heide
authors.initials: H
authors.lastname: Mohs
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33.
authors.forename: Irene
authors.initials: I
authors.lastname: Öchsner
keywords: Developing chicken embryos; Immunohistochemistry; Tissue distribution of LDH5