title: Zebrafish Caudal Haematopoietic Embryonic Stromal Tissue (CHEST) Cells Support Haematopoiesis.
abstract: Haematopoiesis is an essential process in early vertebrate development that occurs in different distinct spatial locations in the embryo that shift over time. These different sites have distinct functions: in some anatomical locations specific hematopoietic stem and progenitor cells (HSPCs) are generated de novo. In others, HSPCs expand. HSPCs differentiate and renew in other locations, ensuring homeostatic maintenance. These niches primarily control haematopoiesis through a combination of cell-to-cell signalling and cytokine secretion that elicit unique biological effects in progenitors. To understand the molecular signals generated by these niches, we report the generation of caudal hematopoietic embryonic stromal tissue (CHEST) cells from 72-hours post fertilization (hpf) caudal hematopoietic tissue (CHT), the site of embryonic HSPC expansion in fish. CHEST cells are a primary cell line with perivascular endothelial properties that expand hematopoietic cells in vitro. Morphological and transcript analysis of these cultures indicates lymphoid, myeloid, and erythroid differentiation, indicating that CHEST cells are a useful tool for identifying molecular signals critical for HSPC proliferation and differentiation in the zebrafish. These findings permit comparison with other temporally and spatially distinct haematopoietic-supportive zebrafish niches, as well as with mammalian haematopoietic-supportive cells to further the understanding of the evolution of the vertebrate hematopoietic system.
authors.affiliation: California State University, Chico, Department of Biological Sciences, Chico, CA, 95929, USA.
authors.forename: Anja
authors.initials: A
authors.lastname: Wolf
authors.affiliation: California State University, Chico, Department of Biological Sciences, Chico, CA, 95929, USA.
authors.forename: Julian
authors.initials: J
authors.lastname: Aggio
authors.affiliation: Department of Cellular and Molecular Medicine, University of California at San Diego School of Medicine, La Jolla, CA, 92093, USA.
authors.forename: Clyde
authors.initials: C
authors.lastname: Campbell
authors.affiliation: California State University, Chico, Department of Biological Sciences, Chico, CA, 95929, USA.
authors.forename: Francis
authors.initials: F
authors.lastname: Wright
authors.affiliation: California State University, Chico, Department of Biological Sciences, Chico, CA, 95929, USA.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Marquez
authors.affiliation: Department of Cellular and Molecular Medicine, University of California at San Diego School of Medicine, La Jolla, CA, 92093, USA.
authors.forename: David
authors.initials: D
authors.lastname: Traver
authors.affiliation: California State University, Chico, Department of Biological Sciences, Chico, CA, 95929, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Stachura
mesh_terms: D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology*
chemical_list: D016222:Fibroblast Growth Factor 2; D012643:Selenium