title: Injection of wild-type cytoplasm and poly(A)+RNA provokes phenotype rescue in spätzle mutant Drosophila embryos.
abstract: spätzle (spz), a maternal effect gene of Drosophila, is involved in the establishment of the dorso-ventral axis during embryogenesis. Eggs from females lacking the spz gene product develop into completely dorsalized embryos, i.e. the ventral and lateral pattern elements fail to develop. Upon injection of either cytoplasm or poly(A)+RNA from early wild-type embryos, spz embryos develop lateral pattern elements represented by Filzkörper and in the case of injected cytoplasm additional ventral pattern elements represented by ventral setae. Wild-type cytoplasm retains the rescuing activity longer than the poly(A)+RNA fraction does, and cytoplasm is always more effective in provoking the rescue than poly(A)+RNA. Mosaic females containing spz germ cells surrounded by spz+tissues were generated by pole cell transplantations; a mutant genotype in the germ cells is sufficient to produce all aspects of the spz mutant phenotype, suggesting that the maternal source of spz gene product is the germ line.
authors.affiliation: Abteilung Biochemie, Max-Planck-Institut für Entwicklungsbiologie, Spemannstraße 35, D-7400, Tübingen, Federal Republic of Germany.
authors.forename: Eveline
authors.initials: E
authors.lastname: Seifert
authors.affiliation: Abteilung Biochemie, Max-Planck-Institut für Entwicklungsbiologie, Spemannstraße 35, D-7400, Tübingen, Federal Republic of Germany.
authors.forename: Felix
authors.initials: F
authors.lastname: Müller-Holtkamp
authors.affiliation: Abteilung Genetik, Max-Planck-Institut für Entwicklungsbiologie, Spemannstraße 35, D-7400, Tübingen, Federal Republic of Germany.
authors.forename: David
authors.initials: D
authors.lastname: Marcey
authors.affiliation: Abteilung Biochemie, Max-Planck-Institut für Entwicklungsbiologie, Spemannstraße 35, D-7400, Tübingen, Federal Republic of Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Jäckle
keywords: Cytoplasm; Dorsalizing mutant; Drosophila; Phenotypic rescue; Poly(A)+ RNA