title: Overexpressed connective tissue growth factor in cardiomyocytes attenuates left ventricular remodeling induced by angiotensin II perfusion.
abstract: To evaluate the improving effects of specifically overexpressed connective tissue growth factor (CTGF) in cardiomyocytes on mice with hypertension induced by angiotensin II (AngII) perfusion, 24 transgenic mice with cardiac-restricted overexpression of CTGF (Tg-CTGF) were divided into two equal groups that were perfused with acetic acid and AngII, respectively, for 7 days. Another 24 cage-control wild-type C57BL/6 mice (NLC) were divided and treated identically. Blood pressure was detected by caudal artery cannulation. Cardiac structural and functional changes were observed by echocardiography. Cardiac fibrosis was detected by Masson staining. After AngII perfusion, blood pressures of NLC and Tg-CTGF mice, especially those of the formers, significantly increased. Compared with NLC + AngII group, Tg-CTGF + AngII group had significantly lower left ventricular posterior wall thickness at end-diastole and left ventricular posterior wall thickness at end-systole as well as significantly higher left ventricular end-systolic diameter and left ventricular end-diastolic diameter (P < 0.05). Reverse transcription-polymerase chain reaction (RT-PCR) showed that Tg-CTGF + AngII group had significantly lower collagen I, α-SMA, and TGF-β mRNA expressions in cardiac tissues (P < 0.05). Tg-CTGF can protect AngII-induced cardiac remodeling of mice with hypertension by mitigating inflammatory response. CTGF may be a therapy target for hypertension-induced myocardial fibrosis, but the detailed mechanism still needs in-depth studies.
authors.affiliation: a First Ward of West Yard , Shaanxi Provincial People's Hospital , Xi'an , Shaanxi Province , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a First Ward of West Yard , Shaanxi Provincial People's Hospital , Xi'an , Shaanxi Province , China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.affiliation: b Department of Cardiovasology , Shaanxi Provincial People's Hospital , Xi'an , Shaanxi Province , China.
authors.forename: Gong-Chang
authors.initials: GC
authors.lastname: Guang
authors.affiliation: b Department of Cardiovasology , Shaanxi Provincial People's Hospital , Xi'an , Shaanxi Province , China.
authors.forename: Zheng-Rong
authors.initials: ZR
authors.lastname: Deng
mesh_terms: D000199:Actins / Q000187:drug effects / Q000235:genetics; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006332:Cardiomegaly / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology; D024042:Collagen Type I / Q000187:drug effects / Q000235:genetics; D055513:Connective Tissue Growth Factor / Q000235:genetics*; D004452:Echocardiography; D005355:Fibrosis; D006321:Heart / Q000000981:diagnostic imaging / Q000187:drug effects; D006352:Heart Ventricles / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D006973:Hypertension / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000235:genetics; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D020257:Ventricular Remodeling / Q000187:drug effects* / Q000235:genetics
chemical_list: D000199:Actins; C526318:CCN2 protein, mouse; D024042:Collagen Type I; D016212:Transforming Growth Factor beta; D014662:Vasoconstrictor Agents; C544013:alpha-smooth muscle actin, mouse; D000804:Angiotensin II; D055513:Connective Tissue Growth Factor
keywords: Angiotensin II; cardiomyocyte; connective tissue growth factor; hypertension; mouse; overexpression