title: Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Antimicrobial Activity against E. coli ATCC 11775, S. maltophilia ATCC 13636 and S. enteritidis ATCC 13076.
abstract: Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides againstEscherichia coliATCC 11775,Stenotrophomonas maltophiliaATCC 13636, andSalmonella enteritidisATCC 13076 was evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The synthetic bovine lactoferricin exhibited antibacterial activity againstE. coliATCC 11775 andS. enteritidisATCC 13076. The dimeric peptide (RRWQWR)₂K-Ahx exhibited the highest antibacterial activity against the tested bacterial strain. The monomeric, cyclic, tetrameric, and palindromic peptides containing the RWQWR motif exhibited high and specific activity againstE. coliATCC 11775. The results suggest that short peptides derived from lactoferricin B could be considered as potential candidates for the development of antibacterial agents against infections caused byE. coli.
authors.affiliation: Chemistry Department, Universidad Nacional de Colombia, Bogotá Carrera 45 No 26-85, Building 451, office 409, Bogotá 11321, Colombia. njhuertasm@unal.edu.co.
authors.forename: Nataly De Jesús
authors.initials: NJ
authors.lastname: Huertas Méndez
authors.affiliation: Bacteriology Department, Universidad Colegio Mayor de Cundinamarca, Bogotá Calle 28 No. 5B-02, Bogotá 110311; Colombia. yerlycasanova@hotmail.com.
authors.forename: Yerly
authors.initials: Y
authors.lastname: Vargas Casanova
authors.affiliation: Bacteriology Department, Universidad Colegio Mayor de Cundinamarca, Bogotá Calle 28 No. 5B-02, Bogotá 110311; Colombia. anyelithgomez_26@hotmail.com.
authors.forename: Anyelith Katherine
authors.initials: AK
authors.lastname: Gómez Chimbi
authors.affiliation: Bacteriology Department, Universidad Colegio Mayor de Cundinamarca, Bogotá Calle 28 No. 5B-02, Bogotá 110311; Colombia. edhernandez@unicolmayor.edu.co.
authors.forename: Edith
authors.initials: E
authors.lastname: Hernández
authors.affiliation: Medicine Faculty, Universidad Nacional de Colombia, Bogotá Carrera 45 No 26-85, Building 471, Bogotá 11321, Colombia. allealc@unal.edu.co.
authors.forename: Aura Lucia
authors.initials: AL
authors.lastname: Leal Castro
authors.affiliation: Chemistry Department, Universidad Nacional de Colombia, Bogotá Carrera 45 No 26-85, Building 451, office 409, Bogotá 11321, Colombia. jmmelod@unal.edu.co.
authors.forename: Javier Mauricio
authors.initials: JM
authors.lastname: Melo Diaz
authors.affiliation: Chemistry Department, Universidad Nacional de Colombia, Bogotá Carrera 45 No 26-85, Building 451, office 409, Bogotá 11321, Colombia. zjriveram@unal.edu.co.
authors.forename: Zuly Jenny
authors.initials: ZJ
authors.lastname: Rivera Monroy
authors.affiliation: Pharmacy Department, Universidad Nacional de Colombia, Bogotá Carrera 45 No 26-85, Building 450, office 203, Bogotá 11321, Colombia. jaegarciaca@unal.edu.co.
authors.forename: Javier Eduardo
authors.initials: JE
authors.lastname: García Castañeda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D002417:Cattle; D002942:Circular Dichroism; D004926:Escherichia coli / Q000187:drug effects*; D007781:Lactoferrin / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; C082171:lactoferricin B; D007781:Lactoferrin
keywords: E. coli; Lactoferricin B; S. enteritidis; S. maltophilia; antibacterial activity; synthetic peptides