title: Endocrine disrupting compounds modulates adiponectin secretion, expression of its receptors and action on steroidogenesis in ovarian follicle.
abstract: We determined the effect of dioxin-like polychlorinated naphtalenes (PCN) (Halowax 1051) (100pg/ml), benzo(a)pyrene [B(a)P] (2.5ng/ml), hexachlorobenzene (HCBz) (0.2ng/ml) and non-dioxin like polybrominated diphenyl ether (BDE-47) (25ng/ml) and bisphenol A (BPA) (20ng/ml) on ovarian adiponectin secretion (ELISA) and its receptors expression (Western blot). Ovarian cells co-culture was used to examine action of endocrine disruptors (EDCs) on adiponectin (10μg/ml) stimulated steroidogenesis. B(a)P, HCBz, testosterone (T) decreased adiponectin secretion and receptors expression, BPA, BDE-47, estradiol (E2) had the opposite effect, while PCN had inhibitory effect only on adiponectin secretion. In adiponectin stimulated cells dioxin-like compounds decreased E2 and except of PCN had no effect on T, while non-dioxin like increased E2 and decreased T secretion. Results indicated to modulatory role of EDCs on adiponectin and its receptor and its action on ovarian steroidogenesis, suggest that dioxin- like compounds may contribute to the ovarian dysfunction in obesity-related disorders.
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Krakow, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Rak
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Krakow, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Zajda
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Krakow, Poland. Electronic address: ewa.gregoraszczuk@uj.edu.pl.
authors.forename: Ewa Łucja
authors.initials: EŁ
authors.lastname: Gregoraszczuk
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000494:pharmacology; D002478:Cells, Cultured; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity; D006581:Hexachlorobenzene / Q000633:toxicity; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity; D009281:Naphthalenes / Q000633:toxicity; D009765:Obesity / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D010636:Phenols / Q000633:toxicity; D054419:Receptors, Adiponectin / Q000378:metabolism*; D013552:Swine; D013739:Testosterone / Q000378:metabolism* / Q000494:pharmacology; D000098826:Bisphenol A Compounds
chemical_list: D052242:Adiponectin; D001559:Benzhydryl Compounds; D001564:Benzo(a)pyrene; D052244:Endocrine Disruptors; D004958:Estradiol; D055768:Halogenated Diphenyl Ethers; D006581:Hexachlorobenzene; D006843:Hydrocarbons, Chlorinated; D009281:Naphthalenes; D010636:Phenols; D054419:Receptors, Adiponectin; D013739:Testosterone; C571106:halowax 1051; C511295:2,2',4,4'-tetrabromodiphenyl ether; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Adiponectin; Endocrine disrupting chemicals; Ovarian follicles; Steroidogenesis