title: Induced Systemic Resistance againstBotrytis cinereabyBacillus cereusAR156 through a JA/ET- andNPR1-Dependent Signaling Pathway and Activates PAMP-Triggered Immunity inArabidopsis.
abstract: Induced resistance response is a potent and cost effective plant defense against pathogen attack. The effectiveness and underlying mechanisms of the suppressive ability byBacillus cereusAR156 toPseudomonas syringaepv.tomatoDC3000 (PstDC3000) inArabidopsishas been investigated previously; however, the strength of induced systemic resistance (ISR) activity againstBotrytis cinerearemains unknown. Here, we show that root-drench application of AR156 significantly reduces disease incidence through activation of ISR. This protection is accompanied with multilayered ISR defense response activated via enhanced accumulation of PR1 protein expression in a timely manner, hydrogen peroxide accumulation and callose deposition, which is significantly more intense in plants with both AR156 pretreatment andB. cinereainoculation than that in plants with pathogen inoculation only. Moreover, AR156 can trigger ISR insid2-2andNahGmutants, but not injar1, ein2andnpr1mutant plants. Our results indicate that AR156-induced ISR depends on JA/ET-signaling pathway andNPR1, but not SA. Also, AR156-treated plants are able to rapidly activate MAPK signaling andFRK1/WRKY53gene expression, both of which are involved in pathogen associated molecular pattern (PAMP)-triggered immunity (PTI). The results indicate that AR156 can induce ISR by the JA/ET-signaling pathways in anNPR1-dependent manner and involves multiple PTI components.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural UniversityNanjing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of EducationNanjing, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Nie
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural UniversityNanjing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of EducationNanjing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural UniversityNanjing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of EducationNanjing, China.
authors.forename: Shune
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural UniversityNanjing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of EducationNanjing, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural UniversityNanjing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of EducationNanjing, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural UniversityNanjing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Ministry of EducationNanjing, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Niu
keywords: Bacillus cereus AR156; ethylene (ET); induced systemic resistance; jasmonic acid (JA); salicylic acid (SA)