title: Patch Angioplasty in the Rat Aorta or Inferior Vena Cava.
abstract: Pericardial patches are commonly used in vascular surgery to close vessels. To facilitate studies of the neointimal hyperplasia that forms on the patch, we developed a rat model of patch angioplasty that can be used in either a vein or an artery, creating a patch venoplasty or a patch arterioplasty, respectively. Technical aspects of this model are discussed. The infra-renal IVC or aorta are dissected and then clamped proximally and distally. A 3 mm venotomy or arteriotomy is performed in the infrarenal inferior vena cava or aorta of 6 to 8 week-old Wistar rats. A bovine pericardial patch (3 mm x 1.5 mm x 0.6 mm) is then used to close the site using a 10-0 nylon suture. Compared to arterial patches, venous patches show increased neointimal thickness on postoperative day 7. This novel model of pericardial patch angioplasty can be used to examine neointimal hyperplasia on vascular biomaterials, as well as to compare the differences between the arterial and venous environments.
authors.affiliation: Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University; Basic Medical College of Zhengzhou University; VA Connecticut Healthcare Systems, West Haven, CT.
authors.forename: Hualong
authors.initials: H
authors.lastname: Bai
authors.affiliation: Department of Vascular Surgery, Xiangya Second Hospital of Central South University, Changsha, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; VA Connecticut Healthcare Systems, West Haven, CT.
authors.forename: Takuya
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; VA Connecticut Healthcare Systems, West Haven, CT.
authors.forename: Haidi
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; VA Connecticut Healthcare Systems, West Haven, CT.
authors.forename: Trenton R
authors.initials: TR
authors.lastname: Foster
authors.affiliation: Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; VA Connecticut Healthcare Systems, West Haven, CT.
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Hanisch
authors.affiliation: Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; VA Connecticut Healthcare Systems, West Haven, CT.
authors.forename: Jeans M
authors.initials: JM
authors.lastname: Santana
authors.affiliation: Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; VA Connecticut Healthcare Systems, West Haven, CT; alan.dardik@yale.edu.
authors.forename: Alan
authors.initials: A
authors.lastname: Dardik
mesh_terms: D017130:Angioplasty / Q000379:methods*; D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery*; D002417:Cattle; D008297:Male; D023421:Models, Animal; D010496:Pericardium / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D014656:Vascular Surgical Procedures / Q000379:methods*; D014682:Vena Cava, Inferior / Q000601:surgery*