title: [Migration of somitic cells into the somatopleural mesoderm of the limb anlage].
abstract: In chick embryos, observations were made on serial semithin transverse sections of the wing level. In addition homo- or heterotopic replacements of the wing or leg somitic mesoderm by labelled somitic or nonsomitic mesoderm were made in 2-to 2.5-day embryos. The nuclear label used was either natural (quail donor embryos in heterotopic transplantations) or isotopic (chick donors labelled with tritiated thymidine).Histological examination revealed that the first somitic cells to leave somite 15 apparently did so at the 20 to 22 somite stage, while the last ones to leave somite 20 apparently did so shortly before the 36 somite stage.Transplantation experiments with labelled donor cells revealed the routes of migratory somitic cells and the time-course of their invasion into the outgrowing limb bud (non-somitic graft cells did not noticeably invade the limb anlage). They showed furthermore that the somitic mesoderm is not regionalized with respect to its limb myogenic properties.These results are compared with those obtained in other classes of vertebrates.
authors.affiliation: Equipe de recherche associée au C.N.R.S. n0 621: «Morphogenèse expérimentale», Laboratoire de Zoologie et Biologie animale, Université scientifique et médicale de Grenoble, B.P. 53, F-38041, Grenoble Cedex, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Chevallier
keywords: Limb development; Migration; Origin of limb musculature; Somitic mesoderm