title: Effect of 5-aminolevulinic acid photodynamic therapy on keratinocyte proliferation and apoptosis in condyloma acuminatum.
abstract: BACKGROUND
  The effect of 5-aminolevulinic acid photodynamic therapy on keratinocyte proliferation and apoptosis in condyloma acuminatum tissues was evaluated.
  
  
  METHODS
  An immunohistochemical method and TdT-mediated dUTP nick end labeling were performed to detect the positive expression of the keratinocyte proliferation-related gene Ki-67 and apoptosis, respectively, in condyloma acuminatum tissues.
  
  
  RESULTS
  Of 52 cases, 44 showed positive expression of Ki-67 in condyloma acuminatum keratinocytes before the treatment, with a positive expression rate of 84.62% (44/52), an expression strength of mostly ++ - +++, and a Ki-67 proliferation index of 80.26±5.07%. After treatment, 22 cases showed positive expression of Ki-67 in condyloma acuminatum keratinocytes, with a positive expression rate of 42.31% (22/52), an expression strength of mostly - - ++, and a proliferation index of 42.67±3.06%. The differences in the positive expression rate, expression strength, and proliferation index in the before- and after-treatment groups were statistically significant (χ2=20.070, P<0.001). For visible apoptotic cells in condyloma acuminatum keratinocytes before the 5-aminolevulinic acid photodynamic treatment, the expression strength was mostly + - ++, and the average apoptotic index was 13.94±2.35%; after treatment, the expression strength was mostly ++ - +++, and the average apoptotic index was 73.88±7.65%; the difference in the apoptotic index between the before and after treatment groups was statistically significant (P<0.001).
  
  
  CONCLUSIONS
  5-Aminolevulinic acid photodynamic therapy can inhibit the proliferation and promote the apoptosis of keratinocytes, and represents an effective mechanism for treating condyloma acuminatum.
authors.affiliation: The First Affiliated Hospital of Zhengzhou University in China, China. Electronic address: gwyin67@126.com.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Yin
authors.affiliation: The First Affiliated Hospital of Zhengzhou University in China, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Sha
authors.affiliation: The First Affiliated Hospital of Zhengzhou University in China, China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Cai
authors.affiliation: The Third Affiliated Hospital of Zhengzhou University in China, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Zhengzhou University in China, China.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Zhengzhou University in China, China.
authors.forename: Xianxian
authors.initials: X
authors.lastname: Xia
authors.affiliation: The First Affiliated Hospital of Zhengzhou University in China, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Pan
mesh_terms: D000293:Adolescent; D000328:Adult; D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003218:Condylomata Acuminata / Q000188:drug therapy* / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D008875:Middle Aged; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid
keywords: 5-aminolevulinic acid photodynamic therapy; Apoptosis; Condyloma acuminatum; Ki-67; Proliferation