title: Genetic and biochemical characterization of hyicin 3682, the first bacteriocin reported for Staphylococcus hyicus.
abstract: Hyicin 3682, the first bacteriocin reported for Staphylococcus hyicus, is a BsaCOLvariant produced by S. hyicus 3682, a strain isolated from bovine milk. Hyicin 3682 is found in the culture supernatant, is bactericidal and its producing strain exhibits a much broader spectrum of antimicrobial activity than the producing strain of BsaCOLagainst several Gram-positive bacteria, which include foodborne pathogens, food-spoilage microorganisms and bacterial species of medical and veterinary importance. Sequencing of the genome of S. hyicus 3682 provided the nucleotide sequence of the entire gene cluster involved in hyicin 3682 production, which seems to be located on pRJ109, the single plasmid carried by this strain. This gene cluster is expressed and consists of 8525bp and of eight genes (hyiA, hyiB, hyiC, hyiD, hyiP, hyiF, hyiE and hyiG) encoded on the same DNA strand. The mature lantibiotic exhibits 91% identity to BsaCOLand its molecular mass was found to be ∼26Da higher due to two amino acid substitutions. S. hyicus 3682 proved to be only partially immune to its cognate bacteriocin up to 1024 AU/ml. Therefore, hyicin 3682, the first Bsa variant reported in coagulase-negative staphylococci, does exhibit antimicrobial and siblicidal activities.
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Carlin Fagundes
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Ilana
authors.initials: I
authors.lastname: Nascimento de Sousa Santos
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Silva Francisco
authors.affiliation: Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Rodolpho
authors.initials: R
authors.lastname: Mattos Albano
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address: mcbastos@micro.ufrj.br.
authors.forename: Maria do Carmo
authors.initials: MD
authors.lastname: de Freire Bastos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D002417:Cattle; D023061:Gene Order; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D057788:Staphylococcus hyicus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins
keywords: Bacteriocin; Gene cluster; Hyicin 3682; Lantibiotic; Staphylococcus hyicus