title: Bioinspired Nanofeatured Substrates: Suitable Environment for Bone Regeneration.
abstract: Bone mimicking coatings provide a complex microenvironment in which material, through its inherent properties (such as nanostructure and composition), affects the commitment of stem cells into bone lineage and the production of bone tissue regulating factors required for bone healing and regeneration. Herein, a bioactive mineral/biopolymer composite made of calcium phosphate/chitosan and hyaluronic acid (CaP-CHI-HA) was elaborated using a versatile simultaneous spray coating of interacting species. The resulting CaP-CHI-HA coating was mainly constituted of bioactive, carbonated and crystalline hydroxyapatite with 277 ± 98 nm of roughness, 1 μm of thickness, and 2.3 ± 1 GPa of stiffness. After five days of culture, CaP-CHI-HA suggested a synergistic effect of intrinsic biophysical features and biopolymers on stem cell mechanobiology and nuclear organization, leading to the expression of an early osteoblast-like phenotype and the production of bone tissue regulating factors such as osteoprotegerin and vascular endothelial growth factor. More interestingly, amalgamation with biopolymers conferred to the mineral a bacterial antiadhesive property. These significant data shed light on the potential regenerative application of CaP-CHI-HA bioinspired coating in providing a suitable environment for stem cell bone regeneration and an ideal strategy to prevent implant-associated infections.
authors.affiliation: EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne , 51100 Reims, France.|UFR d'Odontologie, Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: H
authors.initials: H
authors.lastname: Rammal
authors.affiliation: EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne , 51100 Reims, France.|UFR d'Odontologie, Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: M
authors.initials: M
authors.lastname: Dubus
authors.affiliation: EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne , 51100 Reims, France.|UFR de Pharmacie, Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: L
authors.initials: L
authors.lastname: Aubert
authors.affiliation: EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne , 51100 Reims, France.|UFR de Pharmacie, Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: F
authors.initials: F
authors.lastname: Reffuveille
authors.affiliation: EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne , 51100 Reims, France.|UFR d'Odontologie, Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: D
authors.initials: D
authors.lastname: Laurent-Maquin
authors.affiliation: Plateforme d'Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: C
authors.initials: C
authors.lastname: Terryn
authors.affiliation: INSERM, UMR-S 1121, "Biomatériaux et Bioingénierie", Fédération de médecine translationnelle de Strasbourg, Faculté de Chirurgie Dentaire, Université de Strasbourg , 67000 Strasbourg, France.|CNRS, Institut Charles Sadron UPR 22, Université de Strasbourg , 23 rue du Loess, 67034 Strasbourg Cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Schaaf
authors.affiliation: CNRS, UMR 7198, Institut Jean Lamour (IJL), Université de Lorraine , 54500 Vandoeuvre Lès Nancy, France.
authors.forename: H
authors.initials: H
authors.lastname: Alem
authors.affiliation: CNRS, UMR 7564, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Université de Lorraine , 54500 Vandoeuvre Lès Nancy, France.
authors.forename: G
authors.initials: G
authors.lastname: Francius
authors.affiliation: CNRS, UMR 7564, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Université de Lorraine , 54500 Vandoeuvre Lès Nancy, France.
authors.forename: F
authors.initials: F
authors.lastname: Quilès
authors.affiliation: EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne , 51100 Reims, France.|UFR de Pharmacie, Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: S C
authors.initials: SC
authors.lastname: Gangloff
authors.affiliation: CNRS, Institut Charles Sadron UPR 22, Université de Strasbourg , 23 rue du Loess, 67034 Strasbourg Cedex, France.
authors.forename: F
authors.identifier: http://orcid.org/0000-0002-4934-9276
authors.initials: F
authors.lastname: Boulmedais
authors.affiliation: EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne , 51100 Reims, France.|UFR d'Odontologie, Université de Reims Champagne Ardenne , 51100 Reims, France.
authors.forename: H
authors.identifier: http://orcid.org/0000-0002-8072-9728
authors.initials: H
authors.lastname: Kerdjoudj
mesh_terms: D001861:Bone Regeneration; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D049329:Nanostructures; D010006:Osteoblasts; D010012:Osteogenesis; D013499:Surface Properties; D042461:Vascular Endothelial Growth Factor A
chemical_list: D020099:Coated Materials, Biocompatible; D042461:Vascular Endothelial Growth Factor A; D017886:Durapatite
keywords: bioinspired coating; bone regeneration; human stem cells; mechanobiology; nanomedicine; stem cell differentiation