title: Growth inhibitory activity of Ankaferd hemostat on primary melanoma cells and cell lines.
abstract: OBJECTIVE
  Ankaferd hemostat is the first topical hemostatic agent about the red blood cell-fibrinogen relations tested in the clinical trials. Ankaferd hemostat consists of standardized plant extracts includingAlpinia officinarum, Glycyrrhiza glabra, Thymus vulgaris, Urtica dioica, andVitis vinifera. The aim of this study was to determine the effect of Ankaferd hemostat on viability of melanoma cell lines.
  
  
  METHODS
  Dissimilar melanoma cell lines and primary cells were used in this study. These cells were treated with different concentrations of Ankaferd hemostat to assess the impact of different dosages of the drug. All cells treated with different concentrations were incubated for different time intervals. After the data had been obtained, one-tailed T-test was used to determine whether the Ankaferd hemostat would have any significant inhibitory impact on cell growth.
  
  
  RESULTS
  We demonstrated in this study that cells treated with Ankaferd hemostat showed a significant decrease in cell viability compared to control groups. The cells showed different resistances against Ankaferd hemostat which depended on the dosage applied and the time treated cells had been incubated. We also demonstrated an inverse relationship between the concentration of the drug and the incubation time on one hand and the viability of the cells on the other hand, that is, increasing the concentration of the drug and the incubation time had a negative impact on cell viability.
  
  
  CONCLUSIONS
  The findings in our study contribute to our knowledge about the anticancer impact of Ankaferd hemostat on different melanoma cells.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
authors.forename: Seyhan
authors.initials: S
authors.lastname: Turk
authors.affiliation: Department of Hematology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Umit Yavuz
authors.initials: UY
authors.lastname: Malkan
authors.affiliation: Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ghasemi
authors.affiliation: Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
authors.forename: Helin
authors.initials: H
authors.lastname: Hocaoglu
authors.affiliation: Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Mutlu
authors.affiliation: Department of Hematology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Gursel
authors.initials: G
authors.lastname: Gunes
authors.affiliation: Department of Hematology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Salih
authors.initials: S
authors.lastname: Aksu
authors.affiliation: Department of Hematology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Ibrahim Celalettin
authors.initials: IC
authors.lastname: Haznedaroglu
keywords: Ankaferd hemostat; anticancer; melanoma