title: Comprehensive investigation of aberrant microRNAs expression in cells culture model of MnCl2-induced neurodegenerative disease.
abstract: Manganese (Mn) is required in various human physiological processes. Excessive Mn exposure causes manganism, a progressive neurodegenerative disorder similar to idiopathic Parkinson's disease (IPD). However, the detailed mechanism of Mn-induced neurotoxicity is not yet fully understood. MicroRNAs (miRNAs) play important roles in gene expression regulation, and miRNA expression profile provides additional biological and prognostic information of diseases. In our study, RNA sequencing was performed to profile miRNAs in the SH-SY5Y cells following MnCl2treatment. Expressions of 73 miRNAs were altered following excessive Mn treatment. Furthermore, has-miR-4306 was identified to target 3'UTR of ATP13A2 (PARK9) directly. Inhibition of has-miR-4306 efficiently restored Mn-induced cytotoxicity. Thus, for the first time, we revealed the miRNA effects of Mn ions to neuron cells, highlighted the involvement of miRNA regulation in neurodegeneration caused by Mn exposure, and provided a potential application of miRNAs in future therapeutic intervention.
authors.affiliation: Institute of Biomedical and Pharmaceutical Sciences, Provincial Cooperative Innovation Center, College of Bioengineering, Hubei University of Technology, Wuhan, China.
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.affiliation: Department of Rheumatology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Xie
authors.affiliation: Institute of Biomedical and Pharmaceutical Sciences, Provincial Cooperative Innovation Center, College of Bioengineering, Hubei University of Technology, Wuhan, China.
authors.forename: Linyue
authors.initials: L
authors.lastname: Lv
authors.affiliation: Institute of Biomedical and Pharmaceutical Sciences, Provincial Cooperative Innovation Center, College of Bioengineering, Hubei University of Technology, Wuhan, China.
authors.forename: Yongqi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Biomedical and Pharmaceutical Sciences, Provincial Cooperative Innovation Center, College of Bioengineering, Hubei University of Technology, Wuhan, China.
authors.forename: Xianmin
authors.initials: X
authors.lastname: Xia
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China. Electronic address: xwchen@shou.edu.cn.
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Biomedical and Pharmaceutical Sciences, Provincial Cooperative Innovation Center, College of Bioengineering, Hubei University of Technology, Wuhan, China. Electronic address: zlei0222@gmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002712:Chlorides / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D017895:Manganese Compounds; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D020537:RNA, Small Nucleolar / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C514983:ATP13A2 protein, human; D002712:Chlorides; C000620292:MIRN4306 microRNA, human; D017895:Manganese Compounds; D035683:MicroRNAs; D012335:RNA, Ribosomal; D034741:RNA, Small Interfering; D020537:RNA, Small Nucleolar; D012343:RNA, Transfer; D006180:Proton-Translocating ATPases; C025340:manganese chloride
keywords: ATP13A2; Neurodegeneration; RNA-seq; microRNA; microRNA-4306