title: Novel fluorene-based supramolecular sensor for selective detection of amoxicillin in water and blood.
abstract: Synthesis, characterization and molecular recognition properties of fluorene based supramolecular cleft 1 is reported. The cleft molecule 1 was prepared in a single-step with good yield (85% yield), by linking Fluorene with 1-ethyl piperazine. The cleft molecule 1 was carefully characterized using various spectroscopic techniques such as NMR and mass spectrometry. The supramolecular interaction of cleft 1 with amoxicillin, 6APA, aspirin, captopril, cefotaxime, ceftriaxone, cefuroxime, diclofenac, penicillin, and cephradine was evaluated by fluorescent spectroscopy. The molecular recognition studies showed that amoxicillin selectively binds with cleft 1 in the presence of other drugs. The analytical method developed for the supramolecular interaction of molecular cleft 1 and amoxicillin was validated at varying pH, concentration and temperature during recognition process. Job's plots indicated that the stochiometry of the interactions between the cleft 1 and the amoxicillin was 1:1.
authors.affiliation: International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan. Electronic address: kiramat4s@gmail.com.
authors.forename: Kiramat
authors.initials: K
authors.lastname: Shah
authors.affiliation: Department of Chemistry, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Erum
authors.initials: E
authors.lastname: Hassan
authors.affiliation: International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Farid
authors.initials: F
authors.lastname: Ahmed
authors.affiliation: Department of Chemistry, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Itrat
authors.initials: I
authors.lastname: Anis
authors.affiliation: School of Packaging, Michigan State University, 448 Wilson Road, East Lansing, MI 48824-1223, United States of America (USA).
authors.forename: Muhammad
authors.initials: M
authors.lastname: Rabnawaz
authors.affiliation: International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan. Electronic address: raza.shah@iccs.edu.
authors.forename: Muhammad Raza
authors.initials: MR
authors.lastname: Shah
mesh_terms: D000658:Amoxicillin / Q000032:analysis* / Q000097:blood; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood; D005449:Fluorenes / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008962:Models, Theoretical; D010879:Piperazines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D005449:Fluorenes; D010879:Piperazines; D014874:Water Pollutants, Chemical; D000658:Amoxicillin
keywords: Amoxicillin; Fluorescence spectroscopy; Molecular recognition; Supramolecular hosts