title: Heterotypic induction of stomach-like glands in the urogenital sinus endoderm under the influence of stomach mesenchyme of foetal rats.
abstract: Urogenital sinus endoderm of 16.5-day rat foetuses was combined with stomach mesenchyme and the recombinants were either treated with testosterone and grown in vitro or cultured beneath the kidney capsule of adult male rats of the same strain. It was found that testosterone stimulated mitosis in the urogenital endoderm. In recombinants grown under the kidney capsule a stratified squamous epithelium and stomach-like glands were induced under the influence of the forestomach and glandular stomach mesenchymes. However, the induced glands expressed neither rat pepsinogen nor rat ventral prostatic antigen. They did not produce mRNA for the prostatic steroid-binding protein C1. Thus, stomach mesenchyme of rat foetuses induces organ-specific morphogenesis but not functional differentiation in the heterologous endoderm, indicating that cytodifferentiation does not always accompany morphogenesis.
authors.affiliation: Department of Biology, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, 199-01, Kanagawa, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Mizuno
authors.affiliation: Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, 108, Tokyo, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Furihata
authors.affiliation: Zoological Institute, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takeda
authors.affiliation: Zoological Institute, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Suematsu
authors.affiliation: Strangeways Research Laboratory, Worts Causeway, CB1 4RN, Cambridge, England.
authors.forename: Ilse
authors.initials: I
authors.lastname: Lasnitzki
keywords: Heterotypic induction; Mesenchymal-epithelial interaction; Morphogenesis; Pepsinogen; Stomach