title: Effects of cycloheximide on cellular elongation in aManduca sexta cell line.
abstract: A clone (GV1) of the CHIManduca sexta cell line responds to 20-hydroxy-ecdysone by changing cellular shape from an epithelial-like form to an elongated fibroblast-like form. We have determined that this morphological response to hormone is prevented by treatment with cycloheximide. The inhibition of the elongation response by cycloheximide may relate to a requirement for the synthesis of specific proteins that play a role in the formation of cytoskeletal structure.
authors.affiliation: Insect Attractants, Behavior and Basic Biology Research Laboratory, Agricultural Research, Science and Education Administration, USDA, 32604, Gainesville, Florida, USA.
authors.forename: H
authors.initials: H
authors.lastname: Oberlander
authors.affiliation: Insect Attractants, Behavior and Basic Biology Research Laboratory, Agricultural Research, Science and Education Administration, USDA, 32604, Gainesville, Florida, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Leach
authors.affiliation: Department of Entomology, University of Florida, 32611, Gainesville, Florida, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Lynn
keywords: 20-hydroxy-ecdysone, insect tissue culture; Cycloheximide; Manduca sexta