title: Trypsin-specific Inhibitors from the Macrolepiota procera, Armillaria mellea and Amanita phalloides wild mushrooms.
abstract: Wild growing mushrooms are a rich source of novel proteins with unique features. We have isolated and characterized trypsin inhibitors from two edible mushrooms, the honey fungus (Armillaria mellea) and the parasol mushroom (Macrolepiota procera), and from the poisonous death cap (Amanita phalloides). The trypsin inhibitors isolated: armespin, macrospin and amphaspin, have similar molecular masses, acidic isoelectric points and are not N-glycosylated. They are very strong trypsin inhibitors and weak chymotrypsin inhibitors. They are resistant to exposure to high temperatures and withstand extreme pH values. These exceptional characteristics are advantageous for their potential use in biotechnology, agriculture and medicine.
authors.affiliation: Department of Biotechnology, Jožef Stefan Institute, SI1000 Ljubljana, Slovenia.
authors.forename: Tjaša
authors.initials: T
authors.lastname: Lukanc
authors.affiliation: Department of Biotechnology, Jožef Stefan Institute, SI1000 Ljubljana, Slovenia.
authors.forename: Jože
authors.initials: J
authors.lastname: Brzin
authors.affiliation: Department of Biotechnology, Jožef Stefan Institute, SI1000 Ljubljana, Slovenia.|Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, SI1000 Lubljana, Slovenia.
authors.forename: Janko
authors.initials: J
authors.lastname: Kos
authors.affiliation: Department of Biotechnology, Jožef Stefan Institute, SI1000 Ljubljana, Slovenia.
authors.forename: Jerica
authors.initials: J
authors.lastname: Sabotič
mesh_terms: D000545:Amanita / Q000737:chemistry*; D055392:Armillaria / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D014361:Trypsin Inhibitors
keywords: basidiomycete; inhibitor; mushroom; thermostable; trypsin; trypsin inhibitor