title: Dissecting Pistil Responses to Incompatible and Compatible Pollen in Self-Incompatibility Brassica oleracea Using Comparative Proteomics.
abstract: Angiosperms have developed self-incompatibility (SI) systems to reject self-pollen, thereby promoting outcrossing. The Brassicaceae belongs to typical sporophytic system, having a single S-locus controlled SI response, and was chosen as a model system to study SI-related intercellular signal transduction. In this regard, the downstream factor of EXO70A1 was unknown. Here, protein two-dimensional electrophoresis (2-DE) method and coupled with matrix-assisted laser desorption ionization/time of flight of flight mass spectrometry (MALDI-TOF -MS) and peptide mass fingerprinting (PMF) was used to further explore the mechanism of SI responses in Brassica oleracea L. var. capitata L. at protein level. To further confirm the time point of protein profile change, total proteins were collected from B. oleracea pistils at 0 min, 1 h, and 2 h after self-pollination. In total 902, 1088 and 1023 protein spots were separated in 0 min, 1 h and 2 h 2-DE maps, respectively. Our analyses of self-pollination profiles indicated that proteins mainly changed at 1 h post-pollination in B. oleracea. Moreover, 1077 protein spots were separated in cross-pollinated 1 h (CP) pistil 2-DE map. MALDI-TOF-MS and PMF successfully identified 34 differentially-expressed proteins (DEPs) in SP and CP 1 h 2-DE maps. Gene ontology and KEGG analysis revealed an array of proteins grouped in the following categories: stress and defense response (35%), protein metabolism (18%), carbohydrate and energy metabolism (12%), regulation of translation (9%), pollen tube development (12%), transport (9%) and cytoskeletal (6%). Sets of DEPs identified specifically in SP or only up-regulated expressed in CP pistils were chosen for funther investigating in floral organs and during the process of self- and cross-pollination. The function of these DEPs in terms of their potential involvement in SI in B. oleracea is discussed.
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.affiliation: College of Horticulture and landscape Architecture, Southwest University, Chongqing, 400716, China.
authors.forename: Qiguo
authors.initials: Q
authors.lastname: Gao
authors.affiliation: College of Horticulture and landscape Architecture, Southwest University, Chongqing, 400716, China.
authors.forename: Songmei
authors.initials: S
authors.lastname: Shi
authors.affiliation: College of Horticulture and landscape Architecture, Southwest University, Chongqing, 400716, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lian
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, 45267-0524, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Converse
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Hecui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Horticulture and landscape Architecture, Southwest University, Chongqing, 400716, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Horticulture and landscape Architecture, Southwest University, Chongqing, 400716, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Ren
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China. zhuliquan@swu.edu.cn.
authors.forename: Liquan
authors.initials: L
authors.lastname: Zhu
mesh_terms: D001937:Brassica / Q000737:chemistry* / Q000378:metabolism; D035264:Flowers / Q000378:metabolism*; D010449:Peptide Mapping; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000737:chemistry* / Q000378:metabolism; D054817:Pollination; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins
keywords: Brasssic oleracea; Functional classification; Incompatible and compatible pollination; Pistil proteins