title: Characterization of bioactive chitosan and sulfated chitosan from Doryteuthis singhalensis (Ortmann, 1891).
abstract: Chitosan was extracted from the pen of squid Doryteuthis singhalensis and characterized using FT-IR, NMR, CHN, SEM and DSC analysis. Purified chitosan was sulfated with chlorosulfonic acid in N,N-dimethylformamide and the added sulfate group was confirmed with FT-IR analysis. The molecular weight and degree of deacetylation (DDA) of chitosan was found 226.6kDa and 83.76% respectively. Chitosan exhibited potent antioxidant activity evidenced by reducing power, chelating ability on ferrous ions and scavenging activity on DPPH, superoxide and hydroxyl radicals. The anticoagulant assay using activated partial thromboplastin time (APTT) and prothrombin time (PT) showed chitosan as a strong anticoagulant. The results of this study showed possibility of using D. singhalensis pen as a non-conventional source of natural antioxidants and anticoagulant which can be incorporated in functional food formulations.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India; Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605 014, India. Electronic address: ramkanth281@gmail.com.
authors.forename: Pasiyappazham
authors.initials: P
authors.lastname: Ramasamy
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India; Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603 103, India.
authors.forename: Namasivayam
authors.initials: N
authors.lastname: Subhapradha
authors.affiliation: Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605 014, India.
authors.forename: Thangadurai
authors.initials: T
authors.lastname: Thinesh
authors.affiliation: Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605 014, India.
authors.forename: Joseph
authors.initials: J
authors.lastname: Selvin
authors.affiliation: Department of Ecology & Environmental Sciences, School of Life Sciences, Pondicherry University, Puducherry 605 014, India.
authors.forename: Kanagaraj Muthamizh
authors.initials: KM
authors.lastname: Selvan
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India; Government College of Education, Vellore 632 006, Tamil Nadu, India.
authors.forename: Vairamani
authors.initials: V
authors.lastname: Shanmugam
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India.
authors.forename: Annaian
authors.initials: A
authors.lastname: Shanmugam
mesh_terms: D000107:Acetylation; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D049832:Decapodiformes / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D005609:Free Radicals; C480355:chitosan sulfate; D048271:Chitosan; D007501:Iron
keywords: Anticoagulant; Antioxidant; Chitosan; Deacetylation; Scavenging ability; Squid