title: Characterisation of the human oral microbiome in patients with coronary artery disease using next-generation sequencing of 16SrRNA amplicons.
abstract: BACKGROUND
  Oral health is suspected to be linked to heart disease since species of bacteria that cause periodontitis and dental caries have been found in the atherosclerotic plaque in arteries in the heart.
  
  
  OBJECTIVE
  The aim of this study was to characterize the oral microbiome in patients with coronary artery disease (CAD) and in a patient with dental caries (DC) without any clinical symptoms of CAD.
  
  
  METHODS
  DNA was extracted from the oral swabs collected from the patients and sequencing was performed by next generation sequencing method using Illumina (MiSeq) platform. The resulting sequencing data set was analysed using QIIME.
  
  
  RESULTS
  A total of 31 phyla were found in all the samples. The predominant phylum found in both CAD and DC was Firmicutes (46.09% & 38.98%), Proteobacteria (17.73% & 9.79%), Fusobacteria (13.44% & 17.95%), Bacteroidetes (11.82% & 22.73%), Actinobacteria (8.33% & 7.71%) and TM7 (2.25% & 2.71%). We found a similarity in the bacterial diversity in the two groups of patients.
  
  
  CONCLUSIONS
  A comparison of the oral microbiome in patients with CAD and DC shows a similarity in the composition of the oral microbiota with variations in the proportion of a few genera.
authors.affiliation: Department of Microbiology, Dr. A.L.M. PG Institute of Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu, India.
authors.forename: Thangam
authors.initials: T
authors.lastname: Menon
authors.affiliation: Department of Microbiology, Dr. A.L.M. PG Institute of Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu, India.
authors.forename: Sathya Narayanan
authors.initials: SN
authors.lastname: Gopalakrishnan
authors.affiliation: Department of Microbiology, Dr. A.L.M. PG Institute of Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu, India.
authors.forename: Rayvathy
authors.initials: R
authors.lastname: Balasubramanian
authors.affiliation: Department of Cardiology, Madras Medical College and Rajiv Gandhi General Hospital, Chennai, Tamil Nadu, India.
authors.forename: Stalin Roy
authors.initials: SR
authors.lastname: Justin
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D003324:Coronary Artery Disease; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003731:Dental Caries; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D064307:Microbiota; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S