title: Effect of Whole Grain Qingke (Tibetan Hordeum vulgare L. Zangqing 320) on the Serum Lipid Levels and Intestinal Microbiota of Rats under High-Fat Diet.
abstract: This study investigated the hypolipidemic effect of whole grain Qingke (WGQ) and its influence on intestinal microbiota. Changes in the serum lipid, intestinal environment, and microbiota of Sprague-Dawley rats fed high-fat diets supplemented with different doses of WGQ were determined. Results showed that high doses of WGQ significantly decreased (P < 0.05) the Lee's index, serum total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol levels whereas they increased the body weight of the rats. Cecal weight and short-chain fatty acid (SCFA) concentration increased with increasing WGQ dose. An Illumina-based sequencing approach showed that the relative abundance of putative SCFA-producing bacteria Prevotella and Anaerovibrio increased in the rats fed the WGQ diet. Principal component analysis revealed a significant difference in intestinal microbiota composition after the administration of the WGQ diet. These findings provide insights into the contribution of the intestinal microbiota to the hypolipidemic effect of WGQ.
authors.affiliation: College of Food Science, Southwest University , Chongqing 400715, China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Xia
authors.affiliation: College of Biotechnology, Southwest University , Chongqing 400715, China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Food Science, Southwest University , Chongqing 400715, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Food Science, Southwest University , Chongqing 400715, China.
authors.forename: Tingyuan
authors.initials: T
authors.lastname: Ren
authors.affiliation: College of Food Science, Southwest University , Chongqing 400715, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Zheng
authors.affiliation: College of Food Science, Southwest University , Chongqing 400715, China.
authors.forename: Jianquan
authors.identifier: http://orcid.org/0000-0003-4960-7308
authors.initials: J
authors.lastname: Kan
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D059305:Diet, High-Fat / Q000009:adverse effects; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D001467:Hordeum / Q000737:chemistry / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000067348:Whole Grains / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008055:Lipids
keywords: Illumina MiSeq sequencing; Prevotella; Qingke; serum lipid; short-chain fatty acid