title: BFPTool: a software tool for analysis of Biomembrane Force Probe experiments.
abstract: BACKGROUND
  The Biomembrane Force Probe is an approachable experimental technique commonly used for single-molecule force spectroscopy and experiments on biological interfaces. The technique operates in the range of forces from 0.1 pN to 1000 pN. Experiments are typically repeated many times, conditions are often not optimal, the captured video can be unstable and lose focus; this makes efficient analysis challenging, while out-of-the-box non-proprietary solutions are not freely available.
  
  
  RESULTS
  This dedicated tool was developed to integrate and simplify the image processing and analysis of videomicroscopy recordings from BFP experiments. A novel processing feature, allowing the tracking of the pipette, was incorporated to address a limitation of preceding methods. Emphasis was placed on versatility and comprehensible user interface implemented in a graphical form.
  
  
  CONCLUSIONS
  An integrated analytical tool was implemented to provide a faster, simpler and more convenient way to process and analyse BFP experiments.
authors.affiliation: Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, Prague, 14220 Czech Republic.|Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Kateřinská 2, Prague, 12000 Czech Republic.|Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 9 Quai Saint Bernard, Paris, 75005 France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Šmít
authors.affiliation: Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 9 Quai Saint Bernard, Paris, 75005 France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Fouquet
authors.affiliation: Sorbonne Université, UPMC Univ Paris 06, CNRS, Biological Adaptation and Ageing - Institut de Biologie Paris Seine (B2A - IBPS), 7 Quai Saint Bernard, Paris, 75005 France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Doulazmi
authors.affiliation: Laboratoire de Physique Statistique, École Normale Supérieure, PSL Research University, Paris, France.|Université Paris Diderot Sorbonne Paris Cité, Paris, France.|Sorbonne Universités UPMC Univ Paris 06, CNRS, 24 rue Lhomond, Paris, 75005 France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Pincet
authors.affiliation: Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 9 Quai Saint Bernard, Paris, 75005 France.
authors.forename: Alain
authors.initials: A
authors.lastname: Trembleau
authors.affiliation: Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, Prague, 14220 Czech Republic.|Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Kateřinská 2, Prague, 12000 Czech Republic.
authors.forename: Martin
authors.identifier: 0000-0002-0437-2528
authors.initials: M
authors.lastname: Zapotocky
keywords: Biomembrane Force Probe; Cell mechanics; Force spectroscopy; Image analysis; Motion tracking