title: Microarray technology reveals potentially novel genes and pathways involved in non-functioning pituitary adenomas.
abstract: Microarray data of non-functioning pituitary adenomas (NFPAs) were analyzed to disclose novel genes and pathways involved in NFPA tumorigenesis. Raw microarray data were downloaded from Gene Expression Omnibus. Data pre-treatment and differential analysis were conducted using packages inR. Functional and pathway enrichment analyses were performed using package GOs-tats. A protein-protein interaction (PPI) network was constructed using server STRING and Cytoscape. Known genes involved in pituitary adenomas (PAs), were obtained from the Comparative Toxicogenomics Database. A total of 604 differentially expressed genes (DEGs) were identifed between NFPAs and controls, including 177 up- and 427 down-regulated genes. Jak-STAT and p53 signaling pathways were significantly enriched by DEGs. The PPI network of DEGs was constructed, containing 99 up- and 288 down-regulated known disease genes (e.g. EGFRandESR1) as well as 16 up- and 17 down-regulated potential novel NFPAs-related genes (e.g. COL4A5,LHX3,MSN, andGHSR). Genes likeCOL4A5,LHX3,MSN, andGHSRand pathways such as p53 signaling and Jak-STAT signaling, might participate in NFPA development. Although further validations are required, these findings might provide guidance for future basic and therapy researches.
authors.affiliation: Department of Gastrointestinal Surgery, The Second Hospital of Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Department of Neurosurgery, The Second Hospital of Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Second Hospital of Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Second Hospital of Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, The Second Hospital of Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
keywords: Differentially expressed genes (DEGs); Functional enrichment analysis; Microarray; Non-functioning pituitary adenomas (NFPAs); Protein-protein interaction (PPI)