title: Oligodendrocyte precursor cells in the mouse optic nerve originate in the preoptic area.
abstract: The present study aims to examine the origin of oligodendrocyte progenitor cells (OPCs) in the mouse optic nerve (ON) by labeling OPCs in the fetal forebrain. The labeling of OPCs in the ON was performed by injection of a retrovirus vector carrying the lacZ gene into the lateral ventricle, or by inducible Cre/loxP of Olig2-positive cells. The retrovirus labeling revealed that ventricular zone-derived cells of the fetal forebrain relocated to the ON and differentiated into oligodendrocytes. In addition, lineage tracing of Olig2-positive cells and whole-mount staining of PDGFRα-positive cells demonstrated that OPCs appeared by E12.5 in the preoptic area, and spread caudally to enter the ON. Our results also suggest that OPCs generated during the early stage are depleted from the ON after maturation.
authors.affiliation: Department of Biology, Kyoto Prefectural University of Medicine, Kyoto, 606-0823, Japan. katsono@koto.kpu-m.ac.jp.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ono
authors.affiliation: Departments of Mathematics, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Yoshii
authors.affiliation: Department of Biology, Kyoto Prefectural University of Medicine, Kyoto, 606-0823, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tominaga
authors.affiliation: Department of Biology, Kyoto Prefectural University of Medicine, Kyoto, 606-0823, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Gotoh
authors.affiliation: Department of Biology, Kyoto Prefectural University of Medicine, Kyoto, 606-0823, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nomura
authors.affiliation: Division of Neurobiology and Anatomy, Niigata University, Niigata, Japan.
authors.forename: Hirohide
authors.initials: H
authors.lastname: Takebayashi
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ikenaka
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D005123:Eye / Q000166:cytology; D051379:Mice; D000073637:Oligodendrocyte Precursor Cells / Q000166:cytology*; D000076104:Oligodendrocyte Transcription Factor 2 / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology*; D009900:Optic Nerve / Q000378:metabolism; D011301:Preoptic Area / Q000166:cytology* / Q000196:embryology / Q000378:metabolism
chemical_list: C512773:Olig2 protein, mouse; D000076104:Oligodendrocyte Transcription Factor 2
keywords: CC1; Cre/loxP; EGFP; LacZ; Olig2; PDGFRα; Retrovirus vector