title: Immobilization of a15N-labeled nitrate addition by decomposing forest litter.
abstract: Effects of chronic HNO3and H2SO4additions on decomposition of senesced birch leaf, beech leaf, spruce needle, and wood chip litters were examined. Litters were incubated for up to 4 years in fiberglass mesh (1 mm) bags on experimental plots in a mixed-species forest near the Bear Brooks Watershed Manipulation (BBWM) site in eastern Maine, United States. Plot treatments included HNO3additions at 28 and 56 kg N·ha-1·year-1, H2SO4additions at 128 kg S·ha-1·year-1, and a combined HNO3and H2SO4treatment at 28 kg N and 64 kg S ·ha-1·year-1. The15N content of all NO3added was artificially increased to 344% δ15N. Litter bags were collected each fall and analyzed for organic matter loss, nitrogen concentration, and15N abundance throughout the 4-year experiment. Extractive (non-polar-soluble+water-soluble), cellulose (acid-soluble), and lignin (acid-insoluble) fractions were analyzed for the first 2 years. In wood chips, nitrogen additions increased mass loss and N concentration, but not the mass of N after 4 years. Neither N nor S additions had large effects on mass loss, N concentration, or N content of leaf litters. All litters immobilized and mineralized N simultaneously, but we were able to place a lower bound on gross N immobilization by mass balancing15N additions. Birch and spruce litters showed net mineralization, while beech leaf and wood chip litters showed net immobilization. Net immobilizing litters were those with the highest initial cellulose concentration (wood chips=80% beech leaves=54%), and we attribute the higher capacity for immobilization to more readily available carbon. Lignin mass increased initially in all litter types except spruce needles. Also, extractives in net immobilizing litters either increased initially (wood chips) or decreased at a slower rate than bulk litter (beech leaves). We calculate the potential of decomposing litter to immobilize exogenous nitrate in this system to be 1-1.5 kg N·ha-1·year-1, which is about half of the usual NO3deposition at this site, but only a small fraction of the experimental addition.
authors.affiliation: Marine Biological Laboratory, The Ecosystems Center, 02543, Woods Hole, MA, USA.
authors.forename: Martha R
authors.initials: MR
authors.lastname: Downs
authors.affiliation: Marine Biological Laboratory, The Ecosystems Center, 02543, Woods Hole, MA, USA.
authors.forename: Knute J
authors.initials: KJ
authors.lastname: Nadelhoffer
authors.affiliation: Marine Biological Laboratory, The Ecosystems Center, 02543, Woods Hole, MA, USA.
authors.forename: Jerry M
authors.initials: JM
authors.lastname: Melillo
authors.affiliation: Complex Systems Research Center, University of New Hampshire, 03824, Durham, NH, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Aber
keywords: 15N; Decomposition; N immobilization; N saturation; Nitrate