title: [The HIF1a polymorphism is a diagnostic marker of ischemic stroke].
abstract: To study a variant of the C1772T polymorphism of the hypoxia inducible factor-1a (HIF1a) gene as a molecular genetic predictor of increased risk of ischemic stroke.
  
  
  
  The study included patients with ischemic stroke which was confirmed clinically and by MRI (n=1200), the control group consisted of 500 patients without cardiovascular disease. DNA was extracted from the venous blood using the phenol-chloroform method. Determination of alleles was performed using allele-specific RealTime PCR as well as PCR/RFLP diagnosis.
  
  
  
  The carriage of one or two copies of the allele T significantly increases the risk of ischemic stroke (OR=1.603, p=0.01). At the same time, the minor allele was not associated with the prognosis of the disease outcome.
  
  
  
  The results allow us to consider the HIF1 C1772T polymorphism as a new molecular genetic predictor of ischemic stroke.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Kovalevа
authors.affiliation: Novosibirsk State Medical University of Russian Ministry of Health, Novosibirsk, Russia.
authors.forename: B M
authors.initials: BM
authors.lastname: Doronin
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Morozov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Seryapina
mesh_terms: D000483:Alleles; D002545:Brain Ischemia / Q000175:diagnosis* / Q000235:genetics; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012306:Risk; D020521:Stroke / Q000175:diagnosis* / Q000235:genetics
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit