title: Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice.
abstract: Ovarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.
authors.affiliation: School of Biological Sciences, University of Hong Kong.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Li
authors.affiliation: School of Biological Sciences, University of Hong Kong.
authors.forename: Carman K M
authors.initials: CK
authors.lastname: Ip
authors.affiliation: Department of Mechanical Engineering, University of Hong Kong.
authors.forename: Matthew Y H
authors.initials: MY
authors.lastname: Tang
authors.affiliation: Department of Mechanical Engineering, University of Hong Kong.
authors.forename: Samuel K H
authors.initials: SK
authors.lastname: Sy
authors.affiliation: Department of Medicine, University of Hong Kong.
authors.forename: Susan
authors.initials: S
authors.lastname: Yung
authors.affiliation: Department of Medicine, University of Hong Kong.
authors.forename: Tak-Mao
authors.initials: TM
authors.lastname: Chan
authors.affiliation: Department of Biomedical Sciences, Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong.
authors.forename: Mengsu
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Mechanical Engineering, University of Hong Kong; ashum@hku.hk.
authors.forename: Ho Cheung
authors.initials: HC
authors.lastname: Shum
authors.affiliation: School of Biological Sciences, University of Hong Kong; awong1@hku.hk.
authors.forename: Alice S T
authors.initials: AS
authors.lastname: Wong
mesh_terms: D045744:Cell Line, Tumor; D018450:Disease Progression; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008954:Models, Biological; D009362:Neoplasm Metastasis / Q000175:diagnosis; D010051:Ovarian Neoplasms / Q000473:pathology*; D010529:Peritoneal Cavity / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000175:diagnosis* / Q000556:secondary; D018874:Spheroids, Cellular / Q000473:pathology*; D059016:Tumor Microenvironment