title: A Synthetic Strategy for Conjugation of Paromomycin to Cell-Penetrating Tat(48-60) for Delivery and Visualization into Leishmania Parasites.
abstract: A successful approach to deliver paromomycin, a poorly absorbed aminoglycoside antibiotic, to parasite cells is reported, based on selective protection of amino and hydroxyl groups followed by conjugation to a fluorolabeled, PEG-functionalized cell-penetrating Tat(48-60) peptide. The resulting construct is efficiently internalized into Leishmania cells, evidencing the fitness of cell-penetrating peptides as vectors for efficiently transporting low-bioavailability drugs into cells.
authors.affiliation: Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, 08003 Barcelona, Spain.
authors.forename: Sira
authors.initials: S
authors.lastname: Defaus
authors.affiliation: Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, 08003 Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Gallo
authors.affiliation: Centro de Investigaciones Biológicas (CSIC), 28040 Madrid, Spain.
authors.forename: María A
authors.initials: MA
authors.lastname: Abengózar
authors.affiliation: Centro de Investigaciones Biológicas (CSIC), 28040 Madrid, Spain.
authors.forename: Luis
authors.identifier: 0000-0002-2958-3233
authors.initials: L
authors.lastname: Rivas
authors.affiliation: Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, 08003 Barcelona, Spain.
authors.forename: David
authors.identifier: 0000-0002-6317-6666
authors.initials: D
authors.lastname: Andreu