title: Comprehensive RNA-seq transcriptomic profiling in the malignant progression of gliomas.
abstract: Gliomas are the most common and lethal intracranial tumours. RNA sequencing technologies and advanced data analyses recently enabled the characterization of transcriptomic information, including protein-coding gene expression, non-coding gene expression, alternative splicing, and fusion gene detection, to facilitate detection of diseases and altered phenotypes. As a part of the Chinese Glioma Genome Atlas (CGGA) project, our aim was to delineate comprehensive transcriptome profiling in the malignant progression of human gliomas. Three hundred twenty five gliomas with different grades were collected over the past twelve years. Using the Illumina HiSeq 2,000 system, over 92 million high quality 101-bp paired-end reads were generated per sample, yielding a total of 30 billion reads. This comprehensive dataset will be useful to deepen the comprehensive understanding of gliomas, providing an opportunity to generate new therapies, diagnoses, and preventive strategies.
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Fanlin
authors.initials: F
authors.lastname: Meng
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China.|Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.|Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou 215123, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China.
authors.forename: Chuanbao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China.|Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.|Centre of Brain Tumour, Beijing Institute for Brain Disorders, Beijing 100069, China.|China National Clinical Research Centre for Neurological Diseases, Beijing 100050, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D017398:Alternative Splicing; D020869:Gene Expression Profiling; D005910:Glioma / Q000235:genetics / Q000473:pathology; D006801:Humans; D009362:Neoplasm Metastasis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D012334:RNA, Neoplasm