title: Ameliorative effect of chromium-d-phenylalanine complex on indomethacin-induced inflammatory bowel disease in rats.
abstract: Present study was designed to evaluate the effect of chromium-d-phenylalanine complex (Cr (d-phe)3) on indomethacin-induced inflammatory bowel disease (IBD) in rats. Adult Wistar rats were pretreated with vehicle/Cr (d-phe)3(30, 60 and 90μg/kg, p.o.) for 11days. On day 8 and 9, after one h of the above mentioned treatment, indomethacin (7.5mg/kg/day,s.c.) was administered to induce IBD. On day 12, blood samples were collected from animals for lactate dehydrogenase (LDH) estimation and ileum was isolated for macroscopic scoring, biochemical estimation (lipid peroxidation, reduced glutathione and myeloperoxidase activity) and histopathological study. Administration of indomethacin significantly altered the serum LDH, macroscopic and microscopic appearance and biochemical parameters in ileum tissue. Cr (d-phe)3, at all the tested doses, caused a significant reversal of changes induced by indomethacin. Present study demonstrates the protective effect of Cr (d-phe)3against indomethacin-induced IBD in rats. The observed protective effect might be attributed to the antioxidant and anti-inflammatory properties of Cr (d-phe)3.
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, 572 102, Karnataka, India.
authors.forename: S
authors.initials: S
authors.lastname: Nagarjun
authors.affiliation: D.S.T.S. Mandal's College of Pharmacy, Solapur, 413 004, Maharashtra, India. Electronic address: sharanapharma@hotmail.com.
authors.forename: Shivsharan B
authors.initials: SB
authors.lastname: Dhadde
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, 572 102, Karnataka, India.
authors.forename: Veeresh P
authors.initials: VP
authors.lastname: Veerapur
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, 572 102, Karnataka, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Thippeswamy
authors.affiliation: D.S.T.S. Mandal's College of Pharmacy, Solapur, 413 004, Maharashtra, India.
authors.forename: Baburao N
authors.initials: BN
authors.lastname: Chandakavathe
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D017608:Chromium Compounds / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione; D007213:Indomethacin / Q000633:toxicity*; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced*; D015227:Lipid Peroxidation; D009195:Peroxidase / Q000378:metabolism; D010649:Phenylalanine / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012460:Sulfasalazine / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D017608:Chromium Compounds; D012460:Sulfasalazine; D010649:Phenylalanine; D009195:Peroxidase; D005978:Glutathione; D007213:Indomethacin
keywords: Anti-inflammatory; Antioxidant; Inflammatory bowel disease; Myeloperoxidase