title: A Novel Antimicrobial Endolysin, LysPA26, againstPseudomonas aeruginosa.
abstract: The global increase in multidrug resistant (MDR) bacteria has led to phage therapy being refocused upon. A novel endolysin, LysPA26, containing a lysozyme-like domain, was screened againstPseudomonas aeruginosain this study. It had activity against MDRP. aeruginosawithout pretreatment with an outer-membrane permeabilizer. LysPA26 could kill up to 4 log unitsP. aeruginosain 30 min. In addition, temperature and pH effect assays revealed that LysPA26 had good stability over a broad range of pH and temperatures. Moreover, LysPA26 could kill other Gram-negative bacteria, such asKlebsiella pneumonia, Acinetobacter baumanniiandEscherichia coli, but not Gram-positive bacteria. Furthermore, LysPA26 could eliminateP. aeruginosain biofilm formation. Our current results show that LysPA26 is a new and promising antimicrobial agent for the combat of Gram-negative pathogens.
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Mingquan
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Clinical Medicine, Hangzhou Medical College Hangzhou, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Feng
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Xuran
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Yongzhang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Dong
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Xiaokui
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Qin
keywords: LysPA26; Pseudomonas aeruginosa; antimicrobial agent; bacteriophage; endolysin