title: Transformation of chlorpyrifos in integrated recirculating constructed wetlands (IRCWs) as revealed by compound-specific stable isotope (CSIA) and microbial community structure analysis.
abstract: Carbon isotope analysis and 454 pyrosequencing methods were used to investigate in situ biodegradation of chlorpyrifos during its transport through three model integrated recirculating constructed wetlands (IRCWs). Results show that plant and Fe-impregnated biochar promoted degradation of chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP). Carbon isotope ratios in the IRCWs shifted to -31.24±0.58‰ (IRCW1, plant free), -26.82±0.60‰ (IRCW2, with plant) and -24.76±0.94‰ (IRCW3, with plant and Fe-biochar). The enrichment factors (Ɛbulk,c) were determined as -0.69±0.06‰ (IRCW1), -0.91±0.07‰ (IRCW2) and -1.03±0.09‰ (IRCW3). Microbial community analysis showed that IRCW3 was dominated by members of Bacillus, which can utilize and degrade chlorpyrifos. These results reveal that plant and Fe-biochar can induce carbon isotope fractionation and have a positive impact on the chlorpyrifos degradation efficiency by influencing the development of beneficial microbial communities.
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China; Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou, China. Electronic address: yangyang@jnu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China.
authors.forename: Wenda
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Corp and Soil Sciences, Cornell University, Ithaca, NY 14850, USA.
authors.forename: Murray B
authors.initials: MB
authors.lastname: McBride
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Tao
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China.
authors.forename: Yunv
authors.initials: Y
authors.lastname: Dai
mesh_terms: D001673:Biodegradation, Environmental; D002247:Carbon Isotopes; D004390:Chlorpyrifos; D053833:Wetlands
chemical_list: D002247:Carbon Isotopes; D004390:Chlorpyrifos
keywords: Chlorpyrifos; Compound-specific isotope analysis (CSIA); High-throughput sequencing; Integrated recirculating constructed wetland; Microbial community