title: Phytochemical Profile and Biological Activities of Helianthemum canum l. baumg. from Turkey.
abstract: In the current study, antioxidant, antibacterial activities, and the phenolic compositions of extracts from Helianthemum canum L. Baumg. (Apiaceae) aerial parts were investigated for the first time. The H. canum was extracted with 70% methanol (HCMeOH) and water (HCW). Both extracts were determined by total phenolic contents (3 mg/ml), flavonoids (1.5 mg/ml), flavonols (1.5 mg/ml), qualitative-quantitative compositions, iron (II) chelation activities (0.1 - 5 mg/ml), free radical scavenging activities (DPPH•: 0.01 - 0.6 mg/ml and ABTS+•: 0.125 - 0.5 mg/ml) and the effect upon inhibition of β-carotene/linoleic acid co-oxidation (1 mg/ml). The peroxidation level was also determined using the thiobarbituric acid method (0.01 - 1.5 mg/ml). The results of the activity tests given as IC50values were estimated from non-linear algorithm and compared with standards. Antibacterial activities of extracts and standards were evaluated against Gram-negative and -positive ten standard strains using disc diffusion and broth microdilution methods. The MIC results (312.5 - 2500 μg/ml) against tested microorganisms varied from 625 to 2500 μg/ml. In HPLC analysis, 3,5-dihydroxybenzoic acid was found as the main substance in both extracts. These results showed that HCMeOH was richer in phenolic compounds (284.13 ± 0.30 mg GAE/g extract) from HCW (244.55 ± 0.35 mg GAE/g extract).In conclusion, H. canum extracts showed in vitro antibacterial and antioxidant activities.
authors.affiliation: Department of Pharmaceutical Botany, Erciyes University, TR-38039, Kayseri.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Baldemir
authors.affiliation: Department of Pharmacognosy, Erciyes University, TR-38039, Kayseri.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Gökşen
authors.affiliation: Department of Pharmaceutical Microbiology, Erciyes University, TR-38039, Kayseri.
authors.forename: Nilay
authors.initials: N
authors.lastname: Ildız
authors.affiliation: Department of Pharmacognosy, Erciyes University, TR-38039, Kayseri.
authors.forename: Gökçe Şeker
authors.initials: GŞ
authors.lastname: Karatoprak
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Eastern Mediterranean University, Gazimağusa, K.K.T.C.
authors.forename: Müberra
authors.initials: M
authors.lastname: Koşar
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D030601:Cistaceae / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000302:isolation & purification; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D064209:Phytochemicals / Q000032:analysis* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D012118:Resorcinols / Q000302:isolation & purification; D014421:Turkey
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D062385:Hydroxybenzoates; D007502:Iron Chelating Agents; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts; D012118:Resorcinols; C076950:alpha-resorcylic acid; D000432:Methanol
keywords: 
  Helianthemum canum
  ; 3,5-Dihydroxybenzoic acid; Antibacterial activities; Antioxidant activities; Apiaceae