title: Flavonoids from Potentilla parvifolia Fisch. and Their Neuroprotective Effects in Human Neuroblastoma SH-SY5Y Cells in vitro.
abstract: Potentilla parvifolia Fisch. (Rosaceae) is a traditional medicinal plant in P. R. China. In this study, seven flavonoids, ayanin (1), tricin (2), quercetin (3), tiliroside (4), miquelianin (5), isoquercitrin (6), and astragalin (7), were separated and purified from ethyl acetate extractive fractions from ethanol extracts of P. parvifolia using a combination of sevaral chromatographic methods. The human neuroblastoma SH-SY5Y cells were differentiated with all trans-retinoic acid and treated with okadaic acid to induce tau protein phosphorylation and synaptic atrophy, which could establish an Alzheimer's disease cell model. The neuroprotective effects of these flavonoids in cellular were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3 and 4 had the better neuroprotective effects.
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Guangxiang
authors.initials: G
authors.lastname: Luan
authors.affiliation: Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai, 264005, P. R. China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai, 264005, P. R. China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Hao
authors.affiliation: Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai, 264005, P. R. China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, P. R. China.
authors.forename: Yourui
authors.initials: Y
authors.lastname: Suo
authors.affiliation: Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai, 264005, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, P. R. China.|State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810008, P. R. China.
authors.forename: Honglun
authors.initials: H
authors.lastname: Wang
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology; D018696:Neuroprotective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D010766:Phosphorylation; D010936:Plant Extracts / Q000737:chemistry; D031987:Potentilla / Q000737:chemistry*; D016875:tau Proteins / Q000378:metabolism
chemical_list: D005419:Flavonoids; D018696:Neuroprotective Agents; D010936:Plant Extracts; D016875:tau Proteins
keywords: Flavonoids; Neuroprotective effects; Potentilla parvifolia Fisch.; SH-SY5Y cells; Tau phosphorylation