title: Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats.
abstract: Non-alcoholic fatty liver disease (NAFLD) is the most prevalent form of chronic liver diseases. This study sought to evaluate the insulin-sensitizing effect of paeoniflorin (PF) on high-fat diet-induced NAFLD and possible molecular mechanisms. Male Sprague Dawley rats were fed a high-fat diet (HFD) for 10 weeks to establish the NAFLD model, and PF (20 mg/kg/d) was gavaged to the NAFLD rats for another four weeks. Our results demonstrated that HFD resulted in hepatocellular ballooning, micro-/macrovesicular steatosis, and oxidative stress in the liver, accompanied by increased serum total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels and homeostasis model of insulin resistance (HOMA-IR) index. PF treatment improved the biochemical and histopathological changes in NAFLD rats. Moreover, we also found that PF could inhibit lipid ectopic deposition via regulating lipid metabolism (inhibiting lipid synthesis of cholesterol and de novo pathway), and exert insulin sensitizing effect by regulating the insulin signaling pathway IRS/Akt/GSK3β and anti-oxidation. The study findings suggest that PF has therapeutic potential against NAFLD and that it acts through multiple signaling pathways.
authors.affiliation: Yichun University, Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun, 336000, China.|Hebei University of Chinese Medicine, Department of Immunology and Pathobiology, Shijiazhuang, 050200, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Hebei Medical University, Department of Pharmacology, Shijiazhuang, 050017, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chu
authors.affiliation: Hebei General Hospital, Department of Infectious Diseases, Shijiazhuang, 050051, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Liu
authors.affiliation: The Second Hospital of Hebei Medical University, Department of Surgery, Shijiazhuang, 050000, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Wang
authors.affiliation: Hebei University of Chinese Medicine, Department of Physiology, Shijiazhuang, 050200, China.
authors.forename: Jieru
authors.initials: J
authors.lastname: Li
authors.affiliation: Hebei University of Chinese Medicine, Department of Immunology and Pathobiology, Shijiazhuang, 050200, China.
authors.forename: Wenzao
authors.initials: W
authors.lastname: Yao
authors.affiliation: Yichun University, Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun, 336000, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Yi
authors.affiliation: Beijing Institute of Radiation Medicine, Department of Pharmacology and Toxicology, Beijing, 100850, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D001835:Body Weight; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D007333:Insulin Resistance; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D039821:Monoterpenes / Q000737:chemistry / Q000494:pharmacology*; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D006027:Glycosides
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D019392:Cytochrome P-450 CYP2E1; D005960:Glucosides; D008055:Lipids; D039821:Monoterpenes; D017382:Reactive Oxygen Species; C015423:peoniflorin; D006027:Glycosides