title: Characterization of lettuce big-vein associated virus and Mirafiori lettuce big-vein virus infecting lettuce in Saudi Arabia.
abstract: During 2014 and 2015, 97 lettuce plants that showed big-vein-disease-like symptoms and seven weed plants were collected from the Riyadh region. DAS-ELISA revealed that 25% and 9% of the lettuce plants were singly infected with LBVaV and MiLBVV, respectively, whereas 63% had a mixed infection with both viruses. The results were confirmed by multiplex reverse transcription polymerase chain reaction using primers specific for LBVaV and MiLBVV. LBVaV and MiLBVV were also detected in Sonchus oleraceus and Eruca sativa, respectively. The nucleotide sequence of LBVaV and MiLBVV Saudi isolates ranged from 94.3-100%, and their similarities to isolates with sequences in the GenBank database ranged from 93.9 to 99.6% and 93.8 to 99.3%, respectively. Olpidium sp. was present in the roots of lettuce plants with big-vein disease and it was shown to facilitate transmission of both viruses.
authors.affiliation: Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: M
authors.initials: M
authors.lastname: Umar
authors.affiliation: Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia. ruamerm@ksu.edu.sa.|Viruses and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. ruamerm@ksu.edu.sa.
authors.forename: M A
authors.initials: MA
authors.lastname: Amer
authors.affiliation: Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: M A
authors.initials: MA
authors.lastname: Al-Saleh
authors.affiliation: Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: I M
authors.initials: IM
authors.lastname: Al-Shahwan
authors.affiliation: Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: M T
authors.initials: MT
authors.lastname: Shakeel
authors.affiliation: Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: A M
authors.initials: AM
authors.lastname: Zakri
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 269, 54124, Thessaloniki, Greece.
authors.forename: N I
authors.initials: NI
authors.lastname: Katis
mesh_terms: D018545:Lactuca / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018517:Plant Roots / Q000821:virology; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012529:Saudi Arabia; D012988:Soil Microbiology
keywords: DAS-ELISA; LBVaV; Lettuce; MiLBVV; Olpidium sp; RT-PCR; Sequence