title: Carbapenem nonsusceptibility with modifiedOprDin clinical isolates ofPseudomonas aeruginosafrom India.
abstract: This study was undertaken to investigate OprD porin-mediated carbapenem nonsusceptibility in clinical isolates of Pseudomonas aeruginosa from a tertiary referral hospital of Northeast India. A total of 267 nonduplicate, consecutive clinical isolates of P. aeruginosa were obtained. Mutation and expression levels of OprD gene were determined in carbapenem-nonsusceptible carbapenemase-nonproducing isolates. Among 19 carbapenem-nonsusceptible carbapenemase-nonproducing isolates, 11 of them demonstrated variable band pattern while performing denaturing gradient gel electrophoresis with amplified products of OprD gene. Sequencing of variable band products revealed three mutation patterns in three isolates. Relevant decrease in expression of OprD gene could also be observed in them. All the three isolates exhibited a higher minimum inhibitory concentration for imipenem (64-128 μg/mL) compared to meropenem (16-64 μg/mL). Inactivating mutation and decreased expression of OprD contribute mainly to imipenem resistance as well as to meropenem.
authors.affiliation: Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India.
authors.forename: Debarati
authors.initials: D
authors.lastname: Choudhury
authors.affiliation: Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India.
authors.forename: Anupam Das
authors.initials: AD
authors.lastname: Talukdar
authors.affiliation: Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India.
authors.forename: Manabendra Dutta
authors.initials: MD
authors.lastname: Choudhury
authors.affiliation: Department of Microbiology, Assam University, Silchar, Assam, India.
authors.forename: Anand Prakash
authors.initials: AP
authors.lastname: Maurya
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, Assam, India.
authors.forename: Debadatta Dhar
authors.initials: DD
authors.lastname: Chanda
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, Assam, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Chakravorty
authors.affiliation: Department of Microbiology, Assam University, Silchar, Assam, India.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Bhattacharjee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D004586:Electrophoresis; D020869:Gene Expression Profiling; D060005:Genotyping Techniques; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007194:India; D008826:Microbial Sensitivity Tests; D009154:Mutation; D018272:Porins / Q000235:genetics*; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D062606:Tertiary Care Centers
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D018272:Porins; C077157:OprD protein, Pseudomonas aeruginosa; D015378:Imipenem