title: Respiratory DNA viruses are undetectable in nasopharyngeal secretions from adenotonsillectomized children.
abstract: Respiratory viruses are frequently detected in association with chronic tonsillar hypertrophy in the absence of symptoms of acute respiratory infection (ARI). The present analysis was done in follow-up to a previous clinical study done by this same group. Nasopharyngeal washes (NPWs) were obtained from 83 of 120 individuals at variable times post adenotonsillectomy, in the absence of ARI symptoms. A look back at virus detection results in NPWs from the same 83 individuals at the time of tonsillectomy revealed that 73.5% (61/83) were positive for one or more viruses. The overall frequency of respiratory virus detection in post-tonsillectomy NPWs was 58.8%. Rhinovirus (RV) was the agent most frequently detected, in 38 of 83 subjects (45.8%), followed by enterovirus in 7 (8.4%), human metapneumovirus in 6 (7.2%), human respiratory syncytial virus in 3 (3.6%) and human coronavirus in 1 (1.2%). Remarkably, there was no detection of adenovirus (HAdV) or human bocavirus (HBoV) in asymptomatic individuals in follow-up of adenotonsillectomy. In keeping with persistence of respiratory DNA viruses in human tonsils, tonsillectomy significantly reduces asymptomatic shedding of HAdV and HBoV in NPWs.
authors.affiliation: Departments of Cell Biology, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Ronaldo Bragança
authors.identifier: http://orcid.org/0000-0002-8902-5962
authors.initials: RB
authors.lastname: Martins
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Lucas Penna
authors.initials: LP
authors.lastname: Rocha
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Mirela Moreira
authors.initials: MM
authors.lastname: Prates
authors.affiliation: Departments of Cell Biology, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Talita Bianca
authors.initials: TB
authors.lastname: Gagliardi
authors.affiliation: Departments of Cell Biology, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Balduino
authors.initials: B
authors.lastname: Biasoli
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Marcelo Junqueira
authors.initials: MJ
authors.lastname: Leite
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Buzatto
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Miguel Angelo
authors.initials: MA
authors.lastname: Hyppolito
authors.affiliation: Department of Pediatrics, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Davi Casale
authors.initials: DC
authors.lastname: Aragon
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Edwin
authors.initials: E
authors.lastname: Tamashiro
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Fabiana Cardoso Pereira
authors.initials: FC
authors.lastname: Valera
authors.affiliation: Departments of Cell Biology, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Eurico
authors.initials: E
authors.lastname: Arruda
authors.affiliation: Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Wilma Terezinha
authors.initials: WT
authors.lastname: Anselmo-Lima
mesh_terms: D000234:Adenoids / Q000601:surgery*; D002648:Child; D004267:DNA Viruses / Q000302:isolation & purification*; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D014068:Tonsillectomy