title: Transcription factor Wilms' tumor 1 regulates developmental RNAs through 3' UTR interaction.
abstract: Wilms' tumor 1 (WT1) is essential for the development and homeostasis of multiple mesodermal tissues. Despite evidence for post-transcriptional roles, no endogenous WT1 target RNAs exist. Using RNA immunoprecipitation and UV cross-linking, we show that WT1 binds preferentially to 3' untranslated regions (UTRs) of developmental targets. These target mRNAs are down-regulated upon WT1 depletion in cell culture and developing kidney mesenchyme.Wt1deletion leads to rapid turnover of specific mRNAs. WT1 regulates reporter gene expression through interaction with 3' UTR-binding sites. Combining experimental and computational analyses, we propose that WT1 influences key developmental and disease processes in part through regulating mRNA turnover.
authors.affiliation: Medical Research Council Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom.
authors.forename: Ruthrothaselvi
authors.initials: R
authors.lastname: Bharathavikru
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, United Kingdom.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Dudnakova
authors.affiliation: Medical Research Council Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom.
authors.forename: Stuart
authors.initials: S
authors.lastname: Aitken
authors.affiliation: Medical Research Council Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom.
authors.forename: Joan
authors.initials: J
authors.lastname: Slight
authors.affiliation: Medical Research Council Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom.
authors.forename: Mara
authors.initials: M
authors.lastname: Artibani
authors.affiliation: Medical Research Council Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom.|Roslin Institute, The University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom.
authors.forename: Peter
authors.initials: P
authors.lastname: Hohenstein
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, United Kingdom.
authors.forename: David
authors.initials: D
authors.lastname: Tollervey
authors.affiliation: Medical Research Council Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom.
authors.forename: Nick
authors.initials: N
authors.lastname: Hastie
mesh_terms: D020413:3' Untranslated Regions / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D015536:Down-Regulation; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007668:Kidney / Q000166:cytology; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D000066450:Mouse Embryonic Stem Cells; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D009396:Wilms Tumor / Q000235:genetics* / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger
keywords: 3′ UTR; FLASH; RNA secondary structures; WT1; developmental pathways; hybrids