title: Expression and significance of dendritic cells and Th17/Treg in serum and placental tissues of patients with intrahepatic cholestasis of pregnancy.
abstract: OBJECTIVE
  Dendritic cells (DCs) are involved in immune system, which can also regulate the differentiation of T helper 17 (Th17) and regulatory T cells (Treg). DCs and Th17/Treg participate in preeclampsia and recurrent spontaneous abortion (RSA), but there is still lack of research in intrahepatic cholestasis of pregnancy (ICP). The aim was to evaluate the expression and significance of CD83+DCs, CD1a+DCs, interleukin-17 (IL-17) and IL-35 in serum and placental tissues of patients with ICP.
  
  
  METHODS
  Thirty cases of mild ICP, 25 cases of severe ICP were selected, and 30 cases of normal pregnant women were selected as control group. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) were used to detect the expression of CD83+DCs, CD1a+DCs, IL-17 and IL-35 in serum and placenta tissues, respectively.
  
  
  RESULTS
  There were more CD83+DCs, IL-17 expressed in placenta from women with ICP than in normal pregnancies, while the number of decidual CD1a+DCs, IL-35 was significantly lower in ICP than in normal pregnant women. The comparison within three groups had statistical difference (p < .05). Serum CD83+DCs and CD1a+DCs levels had no significance. IL-17 was higher in ICP, while IL-35 was lower.
  
  
  CONCLUSIONS
  DCs are involved in damaging the maternal-fetal immune tolerance by changing the phenotype and mature state, which may affect the differentiation of Th17/Treg to cause ICP.
authors.affiliation: a Department of Obstetrics and Gynecology , Medical College of Yangzhou University , Yangzhou , China.
authors.forename: Xiang
authors.identifier: http://orcid.org/0000-0002-9657-250X
authors.initials: X
authors.lastname: Kong
authors.affiliation: a Department of Obstetrics and Gynecology , Medical College of Yangzhou University , Yangzhou , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Kong
authors.affiliation: a Department of Obstetrics and Gynecology , Medical College of Yangzhou University , Yangzhou , China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: a Department of Obstetrics and Gynecology , Medical College of Yangzhou University , Yangzhou , China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: a Department of Obstetrics and Gynecology , Medical College of Yangzhou University , Yangzhou , China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D002780:Cholestasis, Intrahepatic / Q000097:blood / Q000145:classification / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D010920:Placenta / Q000276:immunology* / Q000473:pathology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000145:classification / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D007136:Immunoglobulins; D020381:Interleukin-17; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen
keywords: CD1a+DCs; CD83+DCs; Th17/Treg; immune tolerance; intrahepatic cholestasis of pregnancy