title: Mycoplasma genitalium in Spain: prevalence of genital infection and frequency of resistance to macrolides.
abstract: The aim of this study was to determine the prevalence of Mycoplasma genitalium infection and the resistance to macrolides within a general population in Madrid in 2015.
  
  
  
  We collected 359 urine samples from a general population with symptoms of sexually transmitted infections (STIs). All samples underwent a real-time PCR. For the detection of macrolide resistance, a 283bp fragment of region V of the 23S rRNA gene of M. genitalium was amplified and sequenced.
  
  
  
  We found a prevalence of 3.34% of M. genitalium and a macrolide resistance rate of 20%. In males, the prevalence was 6.62% and in women 0.96%, being significantly higher in males.
  
  
  
  The prevalence obtained shows that it is a pathogen to consider in our environment. These findings stress the need for routine testing of M. genitalium infections and would seem to suggest the advisability of resistance testing.
authors.affiliation: Servicio de Microbiología, Hospital Universitario de Getafe, Getafe, Madrid, Spain.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Asenjo
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Johannes G
authors.initials: JG
authors.lastname: Kusters
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Tim T
authors.initials: TT
authors.lastname: Severs
authors.affiliation: Servicio de Microbiología, Hospital Universitario de Getafe, Getafe, Madrid, Spain; Universidad Europea, Villaviciosa de Odón, Madrid, Spain. Electronic address: nachoalos@telefonica.net.
authors.forename: Juan-Ignacio
authors.initials: JI
authors.lastname: Alós
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial; D005260:Female; D005831:Genital Diseases, Female / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D005832:Genital Diseases, Male / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D018942:Macrolides / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009175:Mycoplasma Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D045704:Mycoplasma genitalium / Q000187:drug effects*; D015995:Prevalence; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides
keywords: Bacterial drug resistance; Epidemiology; Epidemiología; Mycoplasma genitalium; Resistencia bacteriana a antibióticos