title: Hypoxia reduces the E-cadherin expression and increases OSCC cell migration regardless of the E-cadherin methylation profile.
abstract: OBJECTIVE
  The purpose of the current study is to investigate the association between E-cadherin methylation status, hypoxia and OSCC.
  
  
  METHODS
  HaCat and SCC9 cell lines were submitted to hypoxic treatment, followed by methylation profile analysis (MS-PCR) and analysis of the expression of mRNA gene E-cadherin (RT-PCR). Study group samples comprise individuals affected by potentially malignant lesions Potential Malignant Oral Lesion (PMOL, n=18) and oral squamous cell carcinoma (OSCC, n=28). The control group oral mucosa (OM, n=15) of patients with an oral mucocele. Cell migration ability was evaluated a scratch wound assay in SCC9 and HaCat cell lines RESULTS: E-cadherin mRNA expression in the cell lines SCC9 and HaCat was significantly reduced under hypoxia, regardless of the methylation profile, when compared to the control group. No differences in methylation profile of the E-cadherin were observed among the groups OM, PMOL and OSCC. HaCat and SCC9 presented increases in cell migration rates under hypoxia.
  
  
  CONCLUSIONS
  The current study demonstrates that hypoxia reduces E-cadherin expression and increase cell migration, regardless of the methylation profile. Additionally, no differences in E-cadherin methylation patterns were observed among OM, PMOL and OSCC.
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Patrícia Luciana Batista
authors.initials: PLB
authors.lastname: Domingos
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Marcela Gonçalves
authors.initials: MG
authors.lastname: Souza
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Talita Antunes
authors.initials: TA
authors.lastname: Guimarães
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Eliane Sobrinho
authors.initials: ES
authors.lastname: Santos
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Lucyana Conceição
authors.initials: LC
authors.lastname: Farias
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: de Carvalho Fraga
authors.affiliation: Dean of Research, Associação Educativa do Brasil (SOEBRAS), Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Kimberly Marie
authors.initials: KM
authors.lastname: Jones
authors.affiliation: Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil.
authors.forename: Sérgio Henrique Souza
authors.initials: SHS
authors.lastname: Santos
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Alfredo Maurício Batista
authors.initials: AMB
authors.lastname: de Paula
authors.affiliation: Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil. Electronic address: andreluizguimaraes@gmail.com.
authors.forename: André Luiz Sena
authors.initials: ALS
authors.lastname: Guimarães
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008745:Methylation; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D012333:RNA, Messenger
keywords: Caspase; HIF-1α; Oral cancer; Proliferation; p53