title: Preparation and characterization of a novel polysialic acid-hyaluronan graft copolymer potential as dermal filler.
abstract: Polysialic acid (PSA) and hyaluronan (HA) are non-immunogenic and biodegradable natural polysaccharides, but HA belongs to glycosaminoglycans and can be immediately degraded by human enzymes. In this study, we synthesized a novel PSA-HA draft copolymer to improve HA stability in vivo. This draft copolymer was characterized by SEM, element analysis and Zeta potential. Cytotoxicity assays, as well as pyrogen and hemolysis tests, were also conducted to test its biological functions further. Results showed that PSA-HA draft copolymer satisfies the medical requirement for biomaterials. In vivo degradation test proved that this copolymer can reduce the irritation rate and prolong the duration of cross-linked HA in skin. These results indicated that PSA-HA draft copolymer can be potentially used as an alternative for free HA in dermal filler (dual-phase cross-linked HA system).
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Fu
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China. Electronic address: xbzhan@yahoo.com.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Zhan
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D000067548:Dermal Fillers / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D006207:Half-Life; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D012794:Sialic Acids / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000378:metabolism
chemical_list: D000067548:Dermal Fillers; D011108:Polymers; D012794:Sialic Acids; C021319:polysialic acid; D006820:Hyaluronic Acid
keywords: Graft copolymer; Hyaluronan; Polysialic acid