title: MiR-409-3p regulates cell proliferation and tumor growth by targeting E74-like factor 2 in osteosarcoma.
abstract: Recent evidence has shown that miR-409-3p was down-regulated in several types of cancer, including osteosarcoma. However, the potential role of miR-409-3p in osteosarcoma remains largely unknown. In the present study, we showed that overexpression of miR-409-3p in osteosarcoma cells inhibited cell proliferationin vitroand suppressed tumor growthin vivo, and the restoration of miR-409-3p promoted G1/S cell cycle arrest and induced cell apoptosis. Additionally, E74-like factor 2 (ELF2) was recognized as a new target of miR-409-3p by dual-luciferase reporter assay. Restoration of ELF2 rescued the inhibitory effect of miR-409-3p on cell proliferation in osteosarcoma cells. Moreover, ELF2 was up-regulated in osteosarcoma tissues and negatively associated with miR-409-3p levels. Taken together, our findings collectively indicate that miR-409-3p may be a tumor suppressor in osteosarcoma and may serve as a promising therapeutic target for osteosarcoma.
authors.affiliation: Department of Orthopaedics The First Affiliated Hospital of Xinxiang Medical College Weihui Henan China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics The First Affiliated Hospital of Xinxiang Medical College Weihui Henan China.
authors.forename: Wengen
authors.initials: W
authors.lastname: Hou
authors.affiliation: Department of Orthopaedics The First Affiliated Hospital of Xinxiang Medical College Weihui Henan China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Orthopaedics The First Affiliated Hospital of Xinxiang Medical College Weihui Henan China.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics The First Affiliated Hospital of Xinxiang Medical College Weihui Henan China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
keywords: E74‐like factor 2; miR‐409‐3p; osteosarcoma; tumor suppressor