title: Overexpression of Rsf-1 correlates with poor survival and promotes invasion in non-small cell lung cancer.
abstract: Rsf-1 (HBXAP) was recently reported to play roles in tumorigenesis and tumor progression. There have been many reports referred to Rsf-1 overexpression in various cancers and associated with the malignant behavior of cancer cells. However, the molecular mechanism of Rsf-1 in non-small cell lung cancer aggressiveness remains ambiguous. In the present study, we found that there was a significant association between Rsf-1 overexpression and poor overall survival (p = 0.028) in lung cancer. Furthermore, knockdown of Rsf-1 expression in H1299 and H460 cells with high endogenous Rsf-1 expression inhibited cell migration and invasion and downregulated MMP2 expression and nuclear levels of NF-κB. NF-κB inhibitor could also block the effect of Rsf-1 in regulation of MMP2 expression. Further experiments demonstrated that Rsf-1 depletion restrained NF-κB reporter luciferase activity and downregulated bcl-2 and p-IκB protein level. In conclusion, we demonstrated that Rsf-1 was overexpressed in lung cancer and associated with poor survival. Rsf-1 regulated cell invasion through MMP2 and NF-κB pathway.
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.|Department of Pathology, The 4th Affiliated Hospital of China Medical University, Shenyang, 110032, China.
authors.forename: Xiuwei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Urology, The 4th Affiliated Hospital of China Medical University, Shenyang, 110032, China.
authors.forename: Dongwei
authors.initials: D
authors.lastname: Xue
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Hao
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Jiani
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Junda
authors.initials: J
authors.lastname: Gai
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China. liqingch@mail.cmu.edu.cn.
authors.forename: Qingchang
authors.identifier: http://orcid.org/0000-0002-5386-9776
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Pathology, The 1st Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, People's Republic of China.
authors.forename: Enhua
authors.initials: E
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000401:mortality / Q000473:pathology*; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000401:mortality / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011379:Prognosis; D015534:Trans-Activators / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D016328:NF-kappa B; D009687:Nuclear Proteins; C452851:RSF1 protein, human; D015534:Trans-Activators; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: MMP2; NF-κB; Non-small cell lung cancer; Rsf-1