title: Daughters from daughterless mothers - Rescuing a female-lethal maternal effect by cytoplasmic transplantation inDrosophila embryos.
abstract: Daughterless is a temperature-sensitive, maternal effect mutation ofDrosophila melanogaster. Homozygousdaughterless females raised at temperatures above 22°C do not produce any female progeny. It was possible to rescue these female embryos by injecting cytoplasm from non-mutant unfertilized eggs into embryos fromdaughterless mothers. Cytoplasm from unfertilized eggs laid by homozygousdaughterless mothers was ineffective. Surprisingly, the cytoplasm from developing embryos with either wild-type ordaughterless mothers could also effect rescue. Based upon this data, we suggest that male and female embryos ofdaughterless mothers differ in their ability to initiate the synthesis of a product during the nuclear multiplication (cleavage) stage of embroniic development in the absence of a putativeda+maternally-synthesized factor, and that this is the basis for the sex-specific action of theda maternal effect.
authors.affiliation: Center for Pathobiology, University of California, Irvine, 92717, Irvine, California, USA.
authors.forename: M
authors.initials: M
authors.lastname: Bownes
authors.affiliation: Center for Pathobiology, University of California, Irvine, 92717, Irvine, California, USA.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Cline
authors.affiliation: Center for Pathobiology, University of California, Irvine, 92717, Irvine, California, USA.
authors.forename: Howard A
authors.initials: HA
authors.lastname: Schneiderman
keywords: Cytoplasmic transplantation; Female-lethal; Maternal effect