title: ELF5-Mediated AR Activation Regulates Prostate Cancer Progression.
abstract: The transcription factor E74-like factor 5 (ELF5) is a potent antioncogene that can prevent epithelial-mesenchymal transition (EMT) and metastasis in prostate cancer (PCa). However, little is known how it suppress the tumor growth and if it can interact with androgen receptor (AR). In this study, we find that the ELF5 is frequently expressed in AR activated PCa cells, where it binds to AR acting as a physiological partner and negatively regulates its transcriptional activity. In addition, the interaction between ELF5 and AR is androgen-dependent. Downregulation of ELF5 by shRNA increases the expression of AR-response genes and the progression of PCa. Moreover, ELF5 is a AR-dependent gene that its expression can be induced by androgen and suppressed by antiandrogen treatment. Notably, forced reduction of ELF5 in LNCaP cells facilitates the binding of AR to ARE in ELF5 gene and enabling its transcription, so that low level ELF5 can turn up its own expression by the negative feedback loop.
authors.affiliation: Department of Urology, Tianjin Institute of Urology, Tianjin Medical University Second Hospital, Tianjin 300211, China.|Department of Urology, Tianjin Third Central Hospital, Tianjin 300170, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Urology, Tianjin Institute of Urology, Tianjin Medical University Second Hospital, Tianjin 300211, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Urology, Tianjin Institute of Urology, Tianjin Medical University Second Hospital, Tianjin 300211, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Urology, Tianjin Institute of Urology, Tianjin Medical University Second Hospital, Tianjin 300211, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Urology, Tianjin Institute of Urology, Tianjin Medical University Second Hospital, Tianjin 300211, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000728:Androgens / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D025461:Feedback, Physiological; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011485:Protein Binding; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000728:Androgens; D004268:DNA-Binding Proteins; C500759:Elf5 protein, mouse; D011944:Receptors, Androgen; D014157:Transcription Factors