title: Influence of hematuria and infection on diagnostic accuracy of urinary LASP1: a new biomarker for bladder carcinoma.
abstract: OBJECTIVE
  To further promote the clinical use of urinary LASP1 as biomarker for urothelial carcinoma of the bladder regarding limitations and alternative testing systems.
  
  
  METHODS
  Urine stabilization, alternative measurement systems and limitations by erythrocyte contamination and infection were investigated in 246 patients.
  
  
  RESULTS
  Thimerosal allowed sufficient stabilization. Fluorescence-activated cell sorting analysis was not influenced by presence of erythrocytes or leukocytes and reliably urothelial carcinoma of the bladder but cell counts in specimen were low. Cut-off values of <25 leukocytes and <200 erythrocytes/µl resulted in sensitivity, specificity, positive and negative predictive values of 0.59, 0.80, 0.80 and 0.59, respectively.
  
  
  CONCLUSIONS
  Hematuria up to 200 erythrocytes/µl but not presence of leukocytes may be tolerated for this promising marker.
authors.affiliation: Institute for Experimental Biomedicine II, University Clinic of Wuerzburg, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Butt
authors.affiliation: Department of Urology, University Hospital Basel, Basel, Switzerland.
authors.forename: Jan
authors.initials: J
authors.lastname: Ebbing
authors.affiliation: Department of Pathology, University Hospital Basel, Basel, Switzerland.
authors.forename: Lukas
authors.initials: L
authors.lastname: Bubendorf
authors.affiliation: Department of Urology, University Hospital Basel, Basel, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Ardelt
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000652:urine*; D000368:Aged; D000369:Aged, 80 and over; D001437:Bacteriuria / Q000150:complications / Q000175:diagnosis*; D014408:Biomarkers, Tumor / Q000652:urine*; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000652:urine*; D003951:Diagnostic Errors; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006417:Hematuria / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D060588:LIM Domain Proteins / Q000652:urine*; D007962:Leukocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011237:Predictive Value of Tests; D001749:Urinary Bladder Neoplasms / Q000150:complications / Q000175:diagnosis* / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins
keywords: FACS; LASP1; biomarker; bladder cancer; urothelial carcinoma