title: Optimizing Fixation Protocols to Improve Molecular Analysis from FFPE Tissues.
abstract: Most Departments of Pathology around the world have a considerable archive of formalin-fixed paraffin-embedded (FFPE) tissue suitable for molecular assessment. This article points out the potential DNA damage that may occur if basic steps are not followed during processing and storage of these samples. Furthermore, it hopes to establish parameters to optimize quality and quantity of DNA extracted from FFPE tissues.
authors.affiliation: School of Dentistry, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Bruna Jalfim
authors.initials: BJ
authors.lastname: Maraschin
authors.affiliation: School of Dentistry, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Viviane Palmeira da
authors.initials: VP
authors.lastname: Silva
authors.affiliation: Faculty of Dentistry, Department of Oral Biological & Medical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Leigha
authors.initials: L
authors.lastname: Rock
authors.affiliation: Oral Cancer Prevention Program, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada.
authors.forename: Huichen
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Dentistry, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Visioli
authors.affiliation: School of Dentistry, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Pantelis Varvaki
authors.initials: PV
authors.lastname: Rados
authors.affiliation: Oral Cancer Prevention Program, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada.
authors.forename: Miriam P
authors.initials: MP
authors.lastname: Rosin
mesh_terms: D006801:Humans; D016612:Paraffin Embedding; D016707:Tissue Fixation