title: A colorimetric aptasensor for sulfadimethoxine detection based on peroxidase-like activity of graphene/nickel@palladium hybrids.
abstract: A sensitive, rapid and label-free colorimetric aptasensor for sulfadimethoxine (SDM) detection was developed based on the tunable peroxidase-like activity of graphene/nickel@palladium nanoparticle (Gr/Ni@Pd) hybrids. The addition of the SDM aptamer could inhibit the peroxidase-like catalytic activity of the hybrids. However, the target SDM and aptamer could be triggered tightly and recover the catalytic activity of the Gr/Ni@Pd hybrids. Due to the peroxidase-like catalytic activity, Gr/Ni@Pd could catalyze the decomposition of H2O2with releasing hydroxyl radicals which further oxidized reagent 3, 3', 5, 5'-Tetramethylbenzidine (TMB) to oxTMB accompanied with a colorless-to-blue color change. The original color change could be applied to obtain quantitative detection of SDM, due to the relationship between the concentration of the target and the color difference. As a result, this approach performed a linear response for SDM from 1 to 500 ng/mL with a limit detection of 0.7 ng/mL (S/N = 3) under the optimized conditions and realized the detection of SDM in spiked lake water samples. Therefore, this colorimetric aptasensor was an alternative assay for SDM detection in real water. Moreover, with its design principle, this work might be applied to detecting other small molecule by employing appropriate aptamer.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Aicheng
authors.initials: A
authors.lastname: Wang
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China. Electronic address: zhaohuim@dlut.edu.cn.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China; Department of Urology, The First Affiliated Hospital of Dalian Medical University, Zhongshan Road 222, Dalian 116027, PR China. Electronic address: chenxiaochi315@163.com.
authors.forename: Xiaochi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Tan
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Yaobin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Xie
authors.initials: X
authors.lastname: Quan
mesh_terms: D052157:Aptamers, Nucleotide; D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D060106:Lakes / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D009532:Nickel / Q000737:chemistry*; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism*; D013412:Sulfadimethoxine / Q000032:analysis*; D014867:Water / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D001560:Benzidines; D014867:Water; D013412:Sulfadimethoxine; C021758:3,3',5,5'-tetramethylbenzidine; D010165:Palladium; D006108:Graphite; D009532:Nickel; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: Aptamer; Colorimetric detection; Graphene/nickel@palladium (Gr/Ni@Pd); Peroxidase-like activity; Sulfadimethoxine (SDM)