title: Anti-Inflammatory Effects of Schisandrin B on LPS-Stimulated BV2 Microglia via Activating PPAR-γ.
abstract: Schisandrin B (Sch B), a dibenzocyclooctadiene lignan isolated from Schisandra chinensis (Turcz.) Baill, has been shown to have anti-inflammatory effect. The purpose of this study was to evaluate the effect of Sch B on LPS-induced inflammation in microglia and to investigate the molecular targets of Sch B. BV2 cells were stimulated by LPS in the presence or absence of Sch B. The results showed that the levels of TNF-α, IL-6, IL-1β, and PGE2upregulated by LPS were significantly suppressed by Sch B. LPS-induced NF-κB activation was also inhibited by Sch B. Furthermore, Sch B was found to upregulate the expression of PPAR-γ in a concentration-dependent manner. In addition, the inhibition of Sch B on TNF-α, IL-6, IL-1β, and PGE2production were reversed by PPAR-γ antagonist GW9662. In conclusion, these results suggested that Sch B inhibited LPS-induced inflammatory response by activating PPAR-γ.
authors.affiliation: Department of Neurology, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212000, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Respiratory, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212001, China. zhengjinxu16@163.com.
authors.forename: Jin-Xu
authors.initials: JX
authors.lastname: Zheng
authors.affiliation: Department of Neurology, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212000, China.
authors.forename: Yuan-Su
authors.initials: YS
authors.lastname: Zhuang
authors.affiliation: Department of Neurology, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212000, China.
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Zhou
authors.affiliation: Department of Neurology, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212000, China.
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Zhao
authors.affiliation: Department of Respiratory, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212001, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002460:Cell Line; D034242:Cyclooctanes / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000517:prevention & control; D017705:Lignans / Q000494:pharmacology*; D008070:Lipopolysaccharides; D017628:Microglia / Q000187:drug effects*; D047495:PPAR gamma / Q000378:metabolism*; D011083:Polycyclic Compounds / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D034242:Cyclooctanes; D017705:Lignans; D008070:Lipopolysaccharides; D047495:PPAR gamma; D011083:Polycyclic Compounds; C015499:schizandrin B
keywords: LPS; NF-κB; PPAR-γ; TNF-α; schisandrin B