title: The Influence of α-Tocopherol on Serum Biochemical Markers During Experimentally Induced Pleuritis in Rats Exposed to Dioxin.
abstract: Toxicity of dioxins is wide ranging. Amongst the organs, the liver is the most susceptible to damage by dioxins. Damage caused to liver cells results in promoting inflammatory processes. The aim of this work was to evaluate whether high doses of tocopherol will change the inflammatory response, monitored by biochemical indicators, by improving liver function in rats exposed to tetrachlorodibenzo-p-dioxin (TCDD). The study was conducted on a population of female Buffalo rats. The animals were divided into the following groups: Control Group A-representing physiological norms for the studied diagnostic indicators; Control Group B-subjects were administered a 1% ceragenin solution to induce pleuritis; Study Group 1-where rats were administered α-tocopherol acetate for 3 weeks, after which pleuritis was induced; Study Group 2-rats were administered a single dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), while 3 weeks later, pleuritis was induced; and Study Group 3-rats were administered a single dose of TCDD and next, were administered α-tocopherol acetate for 3 weeks, followed by pleuritis induction. The results clearly show that administering tocopherol in the course of inflammation causes changes to the distribution and ratio of in the serum protein fractions, including acute phase proteins. The latter proteins are indicative to the improvement in liver function and linked to protein synthesis and stimulation of the antibody-mediated immunity. Moreover, in the course of inflammation caused by exposure of rats to TCDD, tocopherol significantly affected the acute phase protein concentration.
authors.affiliation: Independent Laboratory of Neurotoxicology and Environmental Diagnostics, Wroclaw Medical University, 51-618, Wroclaw, Poland. ireneusz.calkosinski@gmail.com.
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Całkosiński
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wroclaw, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Gostomska-Pampuch
authors.affiliation: Department of Laboratory Diagnostics, 4th Military Hospital, 50-981, Wroclaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Majda
authors.affiliation: Independent Laboratory of Neurotoxicology and Environmental Diagnostics, Wroclaw Medical University, 51-618, Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Leśków
authors.affiliation: Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375, Wroclaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Janeczek
authors.affiliation: Department of Animal Anatomy, National University of Life and Environmental Sciences of Ukraine, Kiev, Ukraine.
authors.forename: Oleg P
authors.initials: OP
authors.lastname: Melnyk
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wroclaw, Poland.|Department of Medical Biochemistry, Wroclaw Medical University, 50-368, Wroclaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D004147:Dioxins / Q000633:toxicity*; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D010998:Pleurisy / Q000139:chemically induced / Q000175:diagnosis / Q000188:drug therapy*; D051381:Rats; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000209:Acute-Phase Proteins; D000975:Antioxidants; D015415:Biomarkers; D004147:Dioxins; D024502:alpha-Tocopherol
keywords: TCDD; dioxin; inflammatory reaction; pleuritis; serum proteins; α-tocopherol