title: Bacteriophage SP6 encodes a second tailspike protein that recognizes Salmonella enterica serogroups C2and C3.
abstract: SP6 is a salmonella phage closely related to coliphage K1-5. K1-5 is notable in that it encodes two polysaccharide-degrading tailspike proteins, an endosialidase that allows it to infect E. coli K1, and a lyase that enables it to infect K5 strains. SP6 is similar to K1-5 except that it encodes a P22-like endorhamnosidase tailspike, gp46, allowing it to infect group B Salmonella. We show here that SP6 can also infect Salmonella serogroups C2and C3and that a mutation in a putative second tailspike, gp47, eliminates this specificity. Gene 47 was fused to the coding region of the N-terminal portion of the Pseudomonas aeruginosa R2 pyocin tail fiber and expressed in trans such that the fusion protein becomes incorporated into pyocin particles. These pyocins, termed AvR2-SP47, killed serogroups C2and C3Salmonella. We conclude that SP6 encodes two tail proteins providing it a broad host range among Salmonella enterica.
authors.affiliation: AvidBiotics Corporation, 100 Kimball Way, S., San Francisco, CA 94080, USA.
authors.forename: Dana
authors.initials: D
authors.lastname: Gebhart
authors.affiliation: AvidBiotics Corporation, 100 Kimball Way, S., San Francisco, CA 94080, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Williams
authors.affiliation: AvidBiotics Corporation, 100 Kimball Way, S., San Francisco, CA 94080, USA. Electronic address: dean@avidbiotics.com.
authors.forename: Dean
authors.initials: D
authors.lastname: Scholl
mesh_terms: D001434:Bacteriophage Typing; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism*; D019779:Salmonella enterica / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism / Q000821:virology*; D065288:Serogroup; D017376:Viral Tail Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017376:Viral Tail Proteins