title: Screen-Printed All-Polymer Aptasensor for Impedance Based Detection of Influenza A Virus.
abstract: In this chapter a detailed description of the fabrication and testing of an aptasensor for influenza A virus detection is given. The sensor chip is an all-polymer chip fabricated with screen-printed poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. Chip substrates are made by CO2laser cutting of Poly(methyl methacrylate) (PMMA) sheets. Influenza A virus specific aptamers are immobilized onto the electrodes by UV cross-linking. Impedance based measurements at a single frequency, measured over time, are used to detect the virus in a buffer solution.
authors.affiliation: DTU Nanotech, Institut for Mikro- og Nanoteknologi, Ørsteds Plads, bygning 345Ø, DK-2800, Kgs. Lyngby, Denmark. julki@nanotech.dtu.dk.
authors.forename: Julie
authors.initials: J
authors.lastname: Kirkegaard
authors.affiliation: DTU Nanotech, Institut for Mikro- og Nanoteknologi, Ørsteds Plads, bygning 345Ø, DK-2800, Kgs. Lyngby, Denmark.
authors.forename: Noemi
authors.initials: N
authors.lastname: Rozlosnik
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D009980:Influenza A virus; D011108:Polymers / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D011108:Polymers
keywords: Aptamers; Aptasensor; Impedance; Influenza A virus; PEDOT:PSS; Screen printing