title: Substitution mapping of QTLs controlling seed dormancy using single segment substitution lines derived from multiple cultivated rice donors in seven cropping seasons.
abstract: A permanent advanced population containing 388 SSSLs was used for genetic analysis of seed dormancy; 25 QTLs including eight stable, six major and five new were identified. Seed dormancy (SD) is not only a complex biological phenomenon, but also a key practical problem in agricultural production closely related with pre-harvest sprouting (PHS). However, the genetic mechanisms of SD remain elusive. Here, we report the genetic dissection of SD in rice using 388 single segment substitution lines (SSSLs) derived from 16 donor parents. Continuous variation and positive correlations in seed germination percentages were observed in seven seasons. Genetic analysis revealed the narrow sense heritability in different seasons varied from 31.4 to 82.2% with an average value of 56.8%. In addition, 49 SSSLs exhibited significant difference to recipient parent HJX74 on SD in at least two seasons, and 12 of them were stably identified with putative QTLs in all of their corresponding cropping seasons. Based on substitution mapping, a total of 25 dormancy QTLs were detected on 11 chromosomes except the chromosome 5 with an interval length of 1.1 to 31.3 cM. The additive effects of these QTLs changed from -0.31 to -0.13, and the additive effect contributions ranged from 16.7 to 41.4%. Six QTLs, qSD3-2, qSD4-1, qSD7-1, qSD7-2, qSD7-3 and qSD11-2, showed large additive effect contributions (≥30%). Five QTLs, qSD3-3, qSD7-1, qSD7-4, qSD9-1 and qSD10-1, may represent novel ones. Furthermore, linkage and recombinant analysis delimited qSD7-1 to a locus 1.5 cM away from marker Oi2 and a 355-kb fragment flanked by RM1134 and Ui159, respectively. Taken together, this work conducts a comprehensive genetic dissection of SD and will provide more selections for breeding elite PHS-resistant rice varieties.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Yuliang
authors.identifier: http://orcid.org/0000-0002-9211-6064
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Xie
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Cai
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Ru
authors.initials: R
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tan
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Guifu
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Shaokui
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Guiquan
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China. zengrz@scau.edu.cn.
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Zeng
mesh_terms: D002874:Chromosome Mapping; D008040:Genetic Linkage; D014644:Genetic Variation; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D057445:Plant Dormancy / Q000235:genetics*; D040641:Quantitative Trait Loci; D012621:Seasons