title: Immunomonitoring reveals interruption of anergy after vaccination in a case of type-2-papillary renal cell carcinoma.
abstract: With the enormous and growing interest in the clinical application of immunotherapy, we are currently facing the need to accurately monitor the immune function of cancer patients. Here, we describe changes in the immune status of a patient with metastatic type-2-papillary renal cell carcinoma, before and after surgery and subsequent immunotherapy with a dendritic cell-tumor cell hybrid vaccine. Through the accurate assessment of monocyte-derived dendritic cells (Mo-DCs) function, we show that Mo-DCs were freed from tumor-induced maturation blockage by tumor resection surgery, while Mo-DCs-tumor induced suppression and anergy were only interrupted by the vaccination treatment. Our data suggest that the evaluation of Mo-DCs' function may provide a powerful and precise tool to monitor immune restoration in cancer patients.
authors.affiliation: Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 1730, São Paulo, SP CEP 05508-900, Brazil.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Clavijo-Salomon
authors.affiliation: Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 1730, São Paulo, SP CEP 05508-900, Brazil.
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Bergami-Santos
authors.affiliation: Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 1730, São Paulo, SP CEP 05508-900, Brazil.|Cell & Molecular Therapy Center NUCEL-NETCEM, University of Sao Paulo, Sao Paulo, SP - Brazil.
authors.forename: José Alexandre
authors.initials: JA
authors.lastname: M Barbuto
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000502:physiology* / Q000637:transplantation; D006801:Humans; D007165:Immunosuppression Therapy; D007167:Immunotherapy / Q000379:methods*; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008297:Male; D015166:Monitoring, Immunologic; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D019139:Tumor Escape / Q000187:drug effects; D014611:Vaccination
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D016207:Cytokines
keywords: immunomonitoring; monocyte-derived dendritic cells; type-2-papillary renal cell carcinoma