title: Multiplex polymerase chain reaction for the detection of high-risk-human papillomavirus types in formalin-fixed paraffin-embedded cervical tissues.
abstract: Detecting high-risk-human papillomavirus (HPV) types has become an integral part of the cervical cancer screening programmes. This study aimed to develop a multiplex polymerase chain reaction (PCR) for identification of HPV types 16 and 18 along with the beta globin gene in formalin-fixed and paraffin-embedded cervical biopsy specimens. A total of 59 samples from patients with cervical abnormalities were tested. HPV 16 positivity was 50% in cervical cancers and 52.9% in cervical intraepithelial neoplasia. Our multiplex PCR protocol can be used as a simple and cost-effective tool for high-risk-HPV detection in cervical cancer screening programmes.
authors.affiliation: Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Mini P
authors.initials: MP
authors.lastname: Singh
authors.affiliation: Department of Cytology and Gynaecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Nalini
authors.initials: N
authors.lastname: Gupta
authors.affiliation: Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: T
authors.initials: T
authors.lastname: Deepak
authors.affiliation: Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Archit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Radha Kanta
authors.initials: RK
authors.lastname: Ratho
mesh_terms: D002278:Carcinoma in Situ / Q000175:diagnosis / Q000821:virology; D002584:Cervix Uteri / Q000821:virology*; D003365:Costs and Cost Analysis; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D057089:Pathology, Molecular / Q000379:methods*; D016707:Tissue Fixation; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000821:virology