title: Bacterial Detection Using Peptide-Based Platform and Impedance Spectroscopy.
abstract: Antimicrobial peptides have the ability to function as bio-recognition elements in the detection of bacteria. For instance, we showed that Leucocin A, an antimicrobial peptide from class IIa bacteriocins, binds gram-positive Listeria monocytogenes with higher affinity than other gram-positive bacteria like S. aureus, L. innocua, and E. faecalis. The binding was detected using impedance spectroscopy when Leucocin A immobilized on impedance electrodes binds bacteria from a sample. Here we highlight the strength of utilizing Leucocin A as a bio-recognition probe in biosensor platforms and provide details on its application in real-time bacterial detection using electrochemical impedance spectroscopy. A simple new generation impedance array analyzer is utilized that works at very low frequencies to identify interactions between peptide and the target bacteria.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada, T6G 2E1.|Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada, T6G 2V4.
authors.forename: Hashem
authors.initials: H
authors.lastname: Etayash
authors.affiliation: Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada, T6G 2V4.
authors.forename: Thomas
authors.initials: T
authors.lastname: Thundat
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada, T6G 2E1. kkaur@chapman.edu.|Chapman University School of Pharmacy (CUSP), Chapman University, Harry and Diane Rinker Health Science Campus, 9401 Jeronimo Road, Irvine, CA, 92618-1908, USA. kkaur@chapman.edu.
authors.forename: Kamaljit
authors.initials: K
authors.lastname: Kaur
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry; D001419:Bacteria / Q000737:chemistry; D001430:Bacteriocins / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods; D008089:Listeria monocytogenes; D010455:Peptides / Q000737:chemistry; D013223:Statistics as Topic / Q000379:methods
chemical_list: D023181:Antimicrobial Cationic Peptides; D001430:Bacteriocins; D010455:Peptides
keywords: Antimicrobial peptide; Bacterial detection; Impedance spectroscopy; Leucocin A; Listeria monocytogenes; Peptide-based biosensors