title: Fabrication of Lab-on-Paper Using Porous Au-Paper Electrode: Application to Tumor Marker Electrochemical Immunoassays.
abstract: A simple, low-cost, and sensitive electrochemical lab-on-paper assay is developed based on a novel gold nanoparticle modified porous paper working electrode for use in point-of-care testing (POCT). Electrochemical methods are introduced for lab-on-paper based on screen-printed paper electrodes. To further improve specificity, performance, and sensitivity for point-of-care testing, a novel porous Au-paper working electrode (Au-PWE) is designed for lab-on-paper using growth of an interconnected Au nanoparticle (NP) layer on the surface of cellulose fibers in order to enhance the conductivity of the paper sample zone and immobilize the primary antibodies (Ab1). With a sandwich-type immunoassay format, Pd-Au bimetallic nanoparticles possessing peroxidase-like activity are used as a matrix to immobilize secondary antibodies (Ab2) for rapid detection of targets. This lab-on-paper based immunodevice is applied to the diagnosis of a cancer biomarker in clinical serum samples.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, No. 336, West Road NanxinZhuang, Jinan, 250022, China.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, No. 336, West Road NanxinZhuang, Jinan, 250022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, No. 336, West Road NanxinZhuang, Jinan, 250022, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, No. 336, West Road NanxinZhuang, Jinan, 250022, China.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Jinan, No. 336, West Road NanxinZhuang, Jinan, 250022, China. ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
mesh_terms: D000906:Antibodies / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D014408:Biomarkers, Tumor; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D018796:Immunoconjugates; D053768:Metal Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis; D010209:Paper; D016062:Porosity; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018796:Immunoconjugates; D006046:Gold
keywords: Electrochemical immunoassay; Lab-on-paper; Porous Au-paper electrode; Tumor marker