title: Methylene blue, curcumin and ion pairing nanoparticles effects on photodynamic therapy of MDA-MB-231 breast cancer cell.
abstract: OBJECTIVE
  The aim of current study was to use methylene blue-curcumin ion pair nanoparticles and single dyes as photosensitizer for comparison of photodynamic therapy (PDT) efficacy on MDA-MB-231 cancer cells, also various light sources effect on activation of photosensitizer (PS) was considered.
  
  
  METHODS
  Ion pair nanoparticles were synthesized using opposite charge ions precipitation and lyophilized. The PDT experiments were designed and the effect of PSs and light sources (Red LED (630nm; power density: 30mWcm-2) and blue LED (465nm; power density: 34mWcm-2)) on the human breast cancer cell line were examined. The effect of PS concentration (0-75μg.mL-1), incubation time, irradiation time and light sources, and priority in irradiation of blue or red lights were determined.
  
  
  RESULTS
  The results show that the ion pairing of methylene blue and curcumin enhance the photodynamic activity of both dyes and the cytotoxicity of ion pair nanoparticles on the MDA-231 breast cancer cell line. Blue and red LED light sources were used for photo activation of photosensitizers. The results demonstrated that both dyes can activate using red light LED better than blue light LED for singlet oxygen producing.
  
  
  CONCLUSIONS
  Nano scale ion pair precipitating of methylene blue-curcumin enhanced the cell penetrating and subsequently cytotoxicity of both dyes together.
authors.affiliation: Medical Laser Research Center, ACECR, Tehran, Iran. Electronic address: r.hoseinzadeh@ut.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Hosseinzadeh
authors.affiliation: Medical Laser Research Center, ACECR, Tehran, Iran; Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
authors.forename: Khatereh
authors.initials: K
authors.lastname: Khorsandi
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008751:Methylene Blue / Q000008:administration & dosage*; D053758:Nanoparticles / Q000008:administration & dosage*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: D017319:Photosensitizing Agents; D003474:Curcumin; D008751:Methylene Blue
keywords: Curcumin; Ion pairing; Methylene blue; Nanoparticle; Photodynamic therapy; Photosensitizer; Singlet oxygen