title: The role of transplanted visceral fat from the long-lived growth hormone receptor knockout mice on insulin signaling.
abstract: Growth hormone receptor knockout mice (GHRKO) are characterized by high insulin sensitivity and extended lifespan. Interestingly, the secretory activity of visceral fat in GHRKO mice is altered, stimulating whole body insulin sensitivity. In this study, we transplanted normal (N) mice with visceral fat pads from GHRKO or N mice to determine the role of visceral fat on the insulin signaling. We found that the transplant of visceral fat from GHRKO mice to N mice (N-GHRKO) improved whole body insulin sensitivity when comparing with sham-operated mice (N-S) and with mice that received visceral fat from N mice (N-N). This was associated with increased hepatic insulin sensitivity as observed by the increased phosphorylated insulin receptor and increased hepatic expression of Pparα and Pparγ. In conclusion, we demonstrated that visceral fat transplant from GHRKO mice into normal mice enhanced insulin sensitivity and glucose tolerance. These results further confirm the differential physiological role played by visceral adipose tissue from GH receptor deficient mice, indicating that the increase of this fat depot can be associated with beneficial effects on insulin signaling and longevity.
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
authors.forename: Mohammed T
authors.initials: MT
authors.lastname: Bennis
authors.affiliation: Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Augusto
authors.initials: A
authors.lastname: Schneider
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
authors.forename: Berta
authors.initials: B
authors.lastname: Victoria
authors.affiliation: Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, 33431, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Do
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.|Department of Medical Biochemistry and Molecular Biology, University of Saarland, 66421, Homburg, Germany.|Department of Biotechnology, University of Applied Sciences Kaiserslautern, 66482, Zweibrücken, Germany.
authors.forename: Denise S
authors.initials: DS
authors.lastname: Wiesenborn
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
authors.forename: Lina
authors.initials: L
authors.lastname: Spinel
authors.affiliation: Department of Oncological Endocrinology, Medical University of Lodz, Lodz, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Gesing
authors.affiliation: Edison Biotechnology Institute, Department of Biomedical Sciences, Ohio University, Athens, OH, 45701, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Kopchick
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
authors.forename: Shadab A
authors.initials: SA
authors.lastname: Siddiqi
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA. michal.masternak@ucf.edu.|Department of Head and Neck Surgery, The Greater Poland Cancer Centre, Poznan, Poland. michal.masternak@ucf.edu.
authors.forename: Michal M
authors.initials: MM
authors.lastname: Masternak
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D005951:Glucose Tolerance Test; D013006:Growth Hormone / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance / Q000235:genetics*; D050152:Intra-Abdominal Fat / Q000637:transplantation*; D008136:Longevity; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010766:Phosphorylation; D012313:RNA / Q000235:genetics*; D011897:Random Allocation; D011986:Receptors, Somatotropin / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D007328:Insulin; D011986:Receptors, Somatotropin; D012313:RNA; D013006:Growth Hormone
keywords: GHR; GHRKO; Insulin resistance; Longevity; Type 2 diabetes