title: Lipid droplets coated with milk fat globule membrane fragments: Microstructure and functional properties as a function of pH.
abstract: Processed lipid droplets coated by milk fat globule membrane (MFGM) material are of primary interest to mimic the specific functions provided by the fat globules in milk and dairy products. The objectives were to investigate, as a function of pH, the properties and microstructure of MFGM-coated lipid droplets prepared with an ingredient rich in MFGM containing polar lipids and proteins. The samples were prepared in water and in milk ultrafiltrate. The combination of microscopy techniques, zeta potential and particle size measurements, and rheological determinations was used. We showed that all the components of the ingredient were highly sensitive to pH. Both the polar lipids and proteins contributed to the isoelectric point of the MFGM-rich ingredient at pI=4.2. Lipid droplets were coated with MFGM fragments both adsorbed at the surface of fat and protruding in the aqueous phase. Below pH5.5 the microstructure of the emulsions was affected by aggregation of the lipid droplets and formation of a gel. The emulsions prepared in water did not show coalescence upon 30days storage, while those prepared in milk ultrafiltrate showed coalescence for pH below 5.5. This study demonstrates that the MFGM-rich ingredient has excellent emulsifying properties and will contribute in the development of emulsions containing MFGM-coated lipid droplets for techno-functional, nutritional and health benefits (e.g. in infant formulas).
authors.affiliation: STLO, INRA, Agrocampus Ouest, 35000 Rennes, France. Electronic address: Christelle.Lopez@inra.fr.
authors.forename: Christelle
authors.initials: C
authors.lastname: Lopez
authors.affiliation: STLO, INRA, Agrocampus Ouest, 35000 Rennes, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Cauty
authors.affiliation: STLO, INRA, Agrocampus Ouest, 35000 Rennes, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Rousseau
authors.affiliation: STLO, INRA, Agrocampus Ouest, 35000 Rennes, France.
authors.forename: Marielle
authors.initials: M
authors.lastname: Blot
authors.affiliation: STLO, INRA, Agrocampus Ouest, 35000 Rennes, France.
authors.forename: Antinéa
authors.initials: A
authors.lastname: Margolis
authors.affiliation: STLO, INRA, Agrocampus Ouest, 35000 Rennes, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Famelart
mesh_terms: D002079:Butter; D045703:Emulsifying Agents / Q000737:chemistry*; D004655:Emulsions; D005511:Food Handling / Q000379:methods*; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D066292:Lipid Droplets / Q000737:chemistry*; D010316:Particle Size; D012212:Rheology; D013499:Surface Properties
chemical_list: D045703:Emulsifying Agents; D004655:Emulsions; D006017:Glycolipids; D006023:Glycoproteins; C110367:milk fat globule; D002079:Butter
keywords: Emulsion; Milk fat globule membrane; Milk polar lipid; Sphingomyelin