title: SERS substrates fabricated using ceramic filters for the detection of bacteria: Eliminating the citrate interference.
abstract: It was found that spectra obtained for bacteria on SERS substrates fabricated by filtering citrate-generated Ag nanoparticles (NPs) onto rigid, ceramic filters exhibited peaks due to citrate as well as the bacteria. In many cases the citrate spectrum overwhelmed that of the bacteria. Given the simplicity of the method to prepare these substrates, means of eliminating this citrate interference were explored. It was found that allowing a mixture of bacteria suspension and citrate-generated Ag NPs to incubate prior to filtering onto the ceramic filter eliminated this interference.
authors.affiliation: GEC, 5101B Backlick Rd., Annandale, VA 22003, United States. Electronic address: pboss@san.rr.com.
authors.forename: P A
authors.initials: PA
authors.lastname: Mosier-Boss
authors.affiliation: SPAWAR Systems Center Pacific, San Diego, CA 92152, United States.
authors.forename: K C
authors.initials: KC
authors.lastname: Sorensen
authors.affiliation: SPAWAR Systems Center Pacific, San Diego, CA 92152, United States.
authors.forename: R D
authors.initials: RD
authors.lastname: George
authors.affiliation: SPAWAR Systems Center Pacific, San Diego, CA 92152, United States.
authors.forename: P C
authors.initials: PC
authors.lastname: Sims
authors.affiliation: San Diego State University Foundation, San Diego, CA 92182, United States.
authors.forename: A
authors.initials: A
authors.lastname: O'braztsova
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000302:isolation & purification*; D019343:Citric Acid / Q000737:chemistry*; D005415:Flavins / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D005415:Flavins; D019343:Citric Acid; D012834:Silver
keywords: Bacteria; Ceramic filter; Citrate; Orbital raster scan; SERS