title: Characterization of a new oda3 allele, oda3-6, defective in assembly of the outer dynein arm-docking complex in Chlamydomonas reinhardtii.
abstract: We have used an insertional mutagenesis approach to generate new C. reinhardtii motility mutants. Of 56 mutants isolated, one is a new allele at the ODA3 locus, called oda3-6. Similar to the previously characterized oda3 alleles, oda3-6 has a slow-jerky swimming phenotype and reduced swimming speed. The oda3-6 mutant fails to assemble the outer dynein arm motor and outer dynein arm-docking complex (ODA-DC) in the ciliary axoneme due to an insertion in the 5' end of the DCC1 gene, which encodes the DC1 subunit of the ODA-DC. Transformation of oda3-6 with the wild-type DCC1 gene rescues the mutant swimming phenotype and restores assembly of the ODA-DC and the outer dynein arm in the cilium. This is the first oda3 mutant to be characterized at the molecular level and is likely to be very useful for further analysis of DC1 function.
authors.affiliation: Department of Biology, Salem State University, Salem, Massachusetts, United States of America.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Brown
authors.affiliation: University of Mississippi Medical Center, Department of Biochemistry, Jackson, Mississippi, United States of America.
authors.forename: Matthew
authors.initials: M
authors.lastname: Mosley
authors.affiliation: Department of Biology, Salem State University, Salem, Massachusetts, United States of America.
authors.forename: Daniela
authors.initials: D
authors.lastname: Montes-Berrueta
authors.affiliation: University of Massachusetts Medical School, Department of Cell and Developmental Biology, Worcester, Massachusetts, United States of America.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Hou
authors.affiliation: University of Mississippi Medical Center, Department of Biochemistry, Jackson, Mississippi, United States of America.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: University of Mississippi Medical Center, Department of Biochemistry, Jackson, Mississippi, United States of America.
authors.forename: Chasity
authors.initials: C
authors.lastname: Scarbrough
authors.affiliation: University of Massachusetts Medical School, Department of Cell and Developmental Biology, Worcester, Massachusetts, United States of America.
authors.forename: George B
authors.initials: GB
authors.lastname: Witman
authors.affiliation: University of Mississippi Medical Center, Department of Biochemistry, Jackson, Mississippi, United States of America.
authors.forename: Maureen
authors.identifier: http://orcid.org/0000-0002-2998-2023
authors.initials: M
authors.lastname: Wirschell
mesh_terms: D000483:Alleles; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism*; D017343:Genes, Plant
chemical_list: D004398:Dyneins