title: Time-course changes of nLDL-induced erectile dysfunction.
abstract: Hyperlipidemia is an important risk factor for atherosclerosis and is frequently seen in patients with erectile dysfunction (ED). This study was designed to evaluate whether the acute effect of native low-density lipoprotein (nLDL) on intracavernosal pressure (ICP) is reversible and related to plasma asymmetrical dimethylarginine (ADMA), endogenous inhibition of endothelial nitric oxide synthase (eNOS) levels and eNOS expression in cavernous tissues. Hyperlipidemia was induced by a single dose of intravenous 4 mg kg-1nLDL. Experiments were performed 72 h (72H), 2 weeks (2W) and 8 weeks (8W) after nLDL injection. Endothelium-dependent relaxations, the ratio of ICP to mean arterial pressure (MAP; ICP/MAP), plasma ADMA levels and eNOS mRNA and protein levels were evaluated. The ICP/MAP ratio decreased in both the 2W and 8W groups. Endothelium-dependent relaxation responses to acetylcholine in the rat thoracic aorta were damaged in the 8W group. Plasma ADMA levels increased in the 8W group. mRNA expression of eNOS decreased in a time-dependent manner, whereas the protein expression increased. These results suggest that acute nLDL injection-induced impairments in erectile functions during an 8-week period are irreversible and might be related to an increase in ADMA levels and changes in the regulation of the eNOS/NO pathway.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Durmus
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Akdeniz, Izmir, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Toylu
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Evcim
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of University of Konya NE, Konya, Turkey.
authors.forename: B C
authors.initials: BC
authors.lastname: Soner
authors.affiliation: Department of Urology, Faculty of Medicine, Dokuz Eylul University, Balcova, Izmir, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Demir
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Dokuz Eylul University, Balcova, Izmir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Kahraman
authors.affiliation: Department of Endocrinology, Faculty of Medicine, Dokuz Eylul University, Balcova, Izmir, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Demir
authors.affiliation: Izmir Metropolitan Municipality Eşrefpaşa Hospital, Clinic of Urology, Izmir, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Irer
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Gidener
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Dokuz Eylul University, Balcova, Izmir, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Atabey
authors.affiliation: Department of Urology, Faculty of Medicine, Dokuz Eylul University, Balcova, Izmir, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Esen
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000503:physiopathology*; D007172:Erectile Dysfunction / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D006949:Hyperlipidemias / Q000150:complications; D008077:Lipoproteins, LDL / Q000009:adverse effects* / Q000097:blood; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D008077:Lipoproteins, LDL; C018524:N,N-dimethylarginine; D001120:Arginine; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat