title: STAT3 potentiates the ability of airway smooth muscle cells to promote angiogenesis by regulating VEGF signalling.
abstract: What is the central question of this study? Airway angiogenesis occurs in asthma, and airway smooth muscle (ASM) cells have been reported to be capable of promoting airway angiogenesis. What is the potential mechanism by which ASM cells harvested from patients with asthma are capable of promoting airway angiogenesis? What is the main finding and its importance? Endogenous STAT3 mediated the pro-angiogenic ability of ASM cells by directly activating VEGF signalling. These findings contribute to the understanding of airway angiogenesis in pathology and could represent a possible therapeutic target for asthma. Airway angiogenesis indicates the specific vascular structure remodelling that occurs in asthma. Airway smooth muscle (ASM) cells have been reported to be capable of promoting airway angiogenesis; however, the potential mechanism is not yet fully defined. Herein, we investigated the role of signal transducer and activator of transcription 3 (STAT3) in the progress of airway angiogenesis. Western blot analysis showed that STAT3 activation was aberrantly upregulated in ASM tissues of patients with asthma and ASM cells that were exposed to cytokines to imitate the airway conditions in patients with asthma. Compared with the control group, both the inhibition of STAT3 activation and the silencing of endogenous STAT3 in ASM cells significantly reduced the proliferation, migration and tube-forming ability of human lung microvascular endothelial cells induced by the conditioned medium (CM) of ASM cells. The increased proliferation and migration of human aortic vascular smooth muscle cells were also repressed by inhibition of STAT3 in ASM cells. Besides, the increased activity of VEGF signalling was observed in ASM cells and the CM by RT-PCR and Western blotting assay, whereas this increased activity was reduced by STAT3 silencing. Further studies indicated that STAT3 regulated VEGF activation by directly interacting with the binding site on the 5' region of the VEGF gene. The increase in STAT3-induced pro-angiogenic activity of ASM cells was significantly decreased by administration of VEGF neutralizing antibody. In conclusion, we provided evidence that endogenous STAT3 mediates the pro-angiogenic ability of ASM cells by directly activating VEGF signalling, which could represent a possible therapeutic target for asthma.
authors.affiliation: Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, China.
authors.forename: Baohua
authors.identifier: 0000-0003-1664-9763
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, China.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Mai
authors.affiliation: Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Du
mesh_terms: D001011:Aorta / Q000378:metabolism / Q000503:physiopathology; D001249:Asthma / Q000378:metabolism / Q000503:physiopathology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000503:physiopathology; D009130:Muscle, Smooth / Q000378:metabolism* / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D050796:STAT3 Transcription Factor; D042461:Vascular Endothelial Growth Factor A
keywords: STAT3; VEGF; airway angiogenesis; airway smooth muscle cells; asthma