title: Eight new γ-lactam alkaloids from the roots of the Hemerocallis minor Mill.
abstract: Eight new γ-lactam alkaloids, hemerominors A-H (1-8), including two pair of epimers (1-4), together with six known compounds (9-14) were isolated from the roots of Hemerocallis minor Mill. The structures of 1-8 were established on the basis of extensive NMR studies and HR-MS measurements as well as comparison with literature data. The absolute configurations of 1-8 were determined by CD spectral analysis and modified Mosher's method. All of compounds were evaluated for their inhibitory effects against LPS-induced NO production in RAW264.7 macrophages. Among them, compound 13 exhibited moderate inhibitory activity against NO production and with IC50value of 18.0 μM.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xin-Cai
authors.initials: XC
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yang-Guo
authors.initials: YG
authors.lastname: Xie
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Fan
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Shi-Kai
authors.initials: SK
authors.lastname: Yan
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: zhangyan_sjtu@sjtu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: kimhz@sjtu.edu.cn.
authors.forename: Hui-Zi
authors.initials: HZ
authors.lastname: Jin
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; Institute of Interdisciplinary Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D031421:Hemerocallis / Q000737:chemistry*; D007769:Lactams / Q000737:chemistry* / Q000302:isolation & purification; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide; D018517:Plant Roots / Q000737:chemistry; D000067996:RAW 264.7 Cells
chemical_list: D000470:Alkaloids; D007769:Lactams; D009569:Nitric Oxide
keywords: Anti-inflammatory activities; Hemerocallis minor Mill.; γ-lactam alkaloids