title: ROS Scavenging and Anti-Wrinkle Effects of Clitocybin A Isolated from the Mycelium of the MushroomClitocybe aurantiaca.
abstract: Clitocybin A, an isoindolinone fromClitocybe aurantiaca, was investigated to assess its anti-wrinkle properties, through reactive oxygen species (ROS)-scavenging and elastase inhibitory activities, procollagen synthesis, and matrix metalloproteinase-1 (MMP-1) expression, in human primary dermal fibroblast-neonatal (HDF-N) cells. Clitocybin A exhibited no significant cytotoxicity up to 10 ppm in HDF-N cells, with cell viability and cell proliferation activity greater than 94.6% and 91.9%, respectively. Strong and concentration-dependent ROS radical scavenging activities of clitocybin A were observed following irradiation with UVB at 30 mJ/cm2. Furthermore, clitocybin A treatment of cells at 0.1, 1, and 10 ppm exhibited decreased elastase activity, in a concentration-dependent manner, by 1.97%, 6.6%, and 8.31%, respectively, versus the control group. The effects of clitocybin A on procollagen synthesis and MMP-1 expression were investigated. Clitocybin A treatment of cells at 1, 5, and 10 ppm increased procollagen synthesis, by 67.9%, 74.4%, and 112.9%, respectively, versus the control group. At these concentrations, MMP-1 expression decreased significantly following UV irradiation. Together, these findings suggest that clitocybin A may be an effective ingredient for use in anti-wrinkle cosmetic products.
authors.affiliation: National Research Laboratory for Cardiovascular Stem Cell, Seoul National University College of Medicine and Department of Internal Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.
authors.forename: Joo-Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors.forename: Ik-Soo
authors.initials: IS
authors.lastname: Lee
authors.affiliation: Innoskin Co., Ltd. BVC, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
authors.forename: Kwan-Chul
authors.initials: KC
authors.lastname: Kim
authors.affiliation: Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
authors.forename: Ick-Dong
authors.initials: ID
authors.lastname: Yoo
authors.affiliation: National Research Laboratory for Cardiovascular Stem Cell, Seoul National University College of Medicine and Department of Internal Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.
authors.forename: Han-Mo
authors.initials: HM
authors.lastname: Yang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D054833:Isoindoles / Q000008:administration & dosage / Q000037:antagonists & inhibitors* / Q000737:chemistry; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects; D025282:Mycelium / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000187:drug effects / Q000378:metabolism; D011347:Procollagen / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism* / Q000528:radiation effects; D012542:Scattering, Radiation; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D016166:Free Radical Scavengers; D054833:Isoindoles; D011347:Procollagen; D017382:Reactive Oxygen Species; C535086:clitocybin A; D010196:Pancreatic Elastase; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: Clitocybin A; anti-wrinkle; elastase; matrix metalloproteinase; procollagen synthesis; reactive oxygen species