title: IgE, IgG1and IgG4response to specific allergens in sensitized subjects showing different clinical reactivity toAnisakis simplex.
abstract: Background.Anisakis simplex hypersensitive subjects may be sensitized without clinical allergy, or experience acute symptoms or chronic urticaria induced by raw fish. We studied whether the 3 subgroups differ in IgE, IgG1or IgG4reactivity to specific Anisakis simplex allergens.Methods.28 Anisakis simplex-hypersensitive adults, 11 with acute symptoms, 9 with chronic urticaria, and 8 sensitized were studied. IgE, IgG1and IgG4to rAni s 1, 5, 9 and 10 were sought by ELISA. IgE and IgG4to nAni s 4 were determined by WB.Results.IgE to Ani s 1, 4, 5, 9, and 10 were found in 8, 3, 2, 5, and 9 sera, respectively. Nine sera did not react to any allergen. IgG1to Ani s 1, 5, 9, and 10 were detected in 5, 16, 14, and 4 sera, respectively. Four sera did not react to any of the 4 allergens. IgG4to Ani s 1, 4, 5, 9, and 10 were detected in 10, 0, 2, 6 and 1 sera, respectively. Fifteen subjects did not react to any of the 5 allergens. On ELISA sensitized subjects showed lower IgE and IgG1levels than patients. IgG4levels were highest in the sensitized group. The prevalence of IgE, IgG1or IgG4reactivity to any of the studied allergens did not differ between the 3 subgroups.Conclusion.The clinical expression of Anisakis simplex sensitization does not seem to depend on IgE reactivity to a specific allergen of the parasite, nor on the presence of IgG antibodies possibly related with blocking activity.
authors.affiliation: Interdisciplinary Department of Medicine (DIM), University of Bari Medical School, Policlinico, Bari, Italy.
authors.forename: M T
authors.initials: MT
authors.lastname: Ventura
authors.affiliation: Hospital La Paz Institute for Health Research, IdiPAZ, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Rodriguez-Perez
authors.affiliation: La Paz Hospital, Allergy Department, IdiPAZ, Madrid, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Caballero
authors.affiliation: Hospital La Paz Institute for Health Research, IdiPAZ, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Garcia-Alonso
authors.affiliation: U.O. Allergologia, Ospedali Riuniti, Ancona, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Antonicelli
authors.affiliation: Ambulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano, Italy. Phone: +39 02 9903 8470 Fax: +39 02 9903 8223 E-mail: r.asero@libero.it.
authors.forename: R
authors.initials: R
authors.lastname: Asero
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017129:Anisakiasis / Q000276:immunology* / Q000469:parasitology; D017163:Anisakis / Q000276:immunology*; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology* / Q000469:parasitology; D016266:Food Parasitology; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D007159:Immunologic Tests; D008297:Male; D008875:Middle Aged; D017747:Seafood / Q000469:parasitology*; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015415:Biomarkers; D015801:Helminth Proteins; D007074:Immunoglobulin G; D007073:Immunoglobulin E
keywords: Allergens; Anisakis simplex; Food allergy; Immune response; Immunoglobulins