title: A review of the methods for storing floral nectars in the field.
abstract: The knowledge of floral nectar sugar characteristics, such as concentration, ratio and mass, is essential to understand the complex nature of nectar production and pollination systems. Although nectar is commonly stored in ecology, storage reliability and effectiveness have rarely been quantified. Inappropriate nectar storage between sampling and analysis can alter nectar chemistry as a result of enzyme or microbial action. Our review of the literature indicates that measures to preserve nectar sugars before analysis include refrigeration, freezing, the addition of an antimicrobial agent, spotting and drying on filter paper, the addition of a desiccant or a combination of these storage treatments. Nectar stored on filter paper is removed by washing with a solvent before analysis. Elution methods are often complex, not standardised and poorly reported in published work. Existing storage methods have generally been used without an evaluation of their impact on results, but evidence suggests a potentially large impact on result accuracy. Future studies should report storage treatments and elution methods to legitimise comparison among independent studies and provide unbiased evaluation of the results. In view of the wide range of storage methods used and lack of verification of their appropriateness, is imperative that standardised and effective methods be developed to ensure that results are reliable. We recommend the prompt analysis of nectar, detailed description of methods, including size of filter paper and method of elution, and use of sterile techniques.
authors.affiliation: Natural and Built Environments, University of South Australia, Mawson Lakes, SA, Australia.
authors.forename: B
authors.initials: B
authors.lastname: Amato
authors.affiliation: Natural and Built Environments, University of South Australia, Mawson Lakes, SA, Australia.
authors.forename: S
authors.identifier: http://orcid.org/0000-0002-7984-5123
authors.initials: S
authors.lastname: Petit
mesh_terms: D035264:Flowers / Q000737:chemistry* / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D057048:Plant Nectar / Q000737:chemistry* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D057048:Plant Nectar; D005632:Fructose; D013395:Sucrose; D005947:Glucose
keywords: Elution; filter paper; nectar storage; nectar sugar; sampling flowers