title: Injury-stimulated and self-restrained BMP signaling dynamically regulates stem cell pool size duringDrosophilamidgut regeneration.
abstract: Many adult organs rely on resident stem cells to maintain homeostasis. Upon injury, stem cells increase proliferation, followed by lineage differentiation to replenish damaged cells. Whether stem cells also change division mode to transiently increase their population size as part of a regenerative program and, if so, what the underlying mechanism is have remained largely unexplored. Here we show that injury stimulates the production of two bone morphogenetic protein (BMP) ligands, Dpp and Gbb, which drive an expansion of intestinal stem cells (ISCs) by promoting their symmetric self-renewing division inDrosophilaadult midgut. We find that BMP production in enterocytes is inhibited by BMP signaling itself, and that BMP autoinhibition is required for resetting ISC pool size to the homeostatic level after tissue repair. Our study suggests that dynamic BMP signaling controls ISC population size during midgut regeneration and reveals mechanisms that precisely control stem cell number in response to tissue needs.
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
authors.forename: Aiguo
authors.identifier: 0000-0001-5011-6034
authors.initials: A
authors.lastname: Tian
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390; jin.jiang@UTSouthwestern.edu.|Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004330:Drosophila / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D012038:Regeneration / Q000235:genetics; D015398:Signal Transduction; D013234:Stem Cells / Q000502:physiology; D013312:Stress, Physiological; D015854:Up-Regulation
chemical_list: D019485:Bone Morphogenetic Proteins; D001761:Bleomycin
keywords: BMP; Dpp; midgut; regeneration; stem cell