title: Gemini-Type Tetraphenylethylene Amphiphiles Containing [12]aneN3and Long Hydrocarbon Chains as Nonviral Gene Vectors and Gene Delivery Monitors.
abstract: Four gemini amphiphiles decorated with triazole-[12]aneN3as the hydrophilic moiety and various long hydrocarbons as hydrophobic moieties, 1-4, were designed to form micelles possessing the aggregation-induced emission (AIE) property for gene delivery and tracing. All four amphiphiles give ultralow critical micelle concentrations, are pH-/photostable and biocompatible, and completely retard the migration of plasmid DNAs at low concentrations. The DNA-binding abilities of the micelles were fully assessed. The coaggregated nanoparticles of 1-4 with DNAs could convert back into AIE micelles. In vitro transfections indicated that lipids 1 and 2 and their originated liposomes bearing decent delivering abilities have great potentials as nonviral vectors. Finally, on the basis of the transfection and the transitions between condensates and micelles, lipid 2 was singled out as the first example for real-time tracing of the intracellular deliveries of nonlabeled DNA, which provides spatiotemporal messages about the processes of condensate uptake and DNA release.
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University , Beijing 100875, China.|College of Chemistry and Chemical Engineering, Xinyang Normal University , Xinyang 464000, China.
authors.forename: Ai-Xiang
authors.initials: AX
authors.lastname: Ding
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University , Beijing 100875, China.
authors.forename: Zheng-Li
authors.initials: ZL
authors.lastname: Tan
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University , Beijing 100875, China.
authors.forename: You-Di
authors.initials: YD
authors.lastname: Shi
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University , Beijing 100875, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University , Beijing 100875, China.|Department of Chemistry, State University of New York , Buffalo, New York 14260, United States.
authors.forename: Bing
authors.initials: B
authors.lastname: Gong
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University , Beijing 100875, China.
authors.forename: Zhong-Lin
authors.identifier: http://orcid.org/0000-0001-5473-2889
authors.initials: ZL
authors.lastname: Lu
mesh_terms: D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006838:Hydrocarbons; D008055:Lipids; D008823:Micelles; D013267:Stilbenes / Q000737:chemistry*; D014162:Transfection
chemical_list: D006838:Hydrocarbons; D008055:Lipids; D008823:Micelles; D013267:Stilbenes; C000617116:tetraphenylethylene
keywords: AIE micelles; gemini lipids; gene delivery tracing; micelle and nanoparticle transition; nonviral gene vectors