title: Tetraspanin CD151 mediates communication between PC3 prostate cancer cells and osteoblasts.
abstract: Invasion and migration of cancer cells are crucial for the formation of secondary lesions. These require activation of signalling cascades modulated by the number of regulatory molecules. One such molecule is CD151, a member of evolutionary conserved tetraspanin family. CD151 is involved in cell adhesion, motility and cancer progression due to formation of complexes with laminin-binding integrins and regulation of growth factor receptors function (e.g. HGFR, TGFβR, EGFR). Recent studies point to correlation between CD151 expression and high tumour grade in prostate cancer (PCa). Herein, we investigated a possible role of CD151 in communication between PC3 cancer cells and either cancer-associated fibroblasts (CAFs) or osteoblasts, an interplay which is significant for metastasis. The analysis showed that although CAFs strongly enhanced both migration and invasion of PC3 prostate cancer cells, the effect was not dependent on CD151. On the other hand, CD151 was found to promote 3D migration as well as invasive growth in response to osteoblasts-secreted growth factors. Obtained data revealed that knockdown of CD151 abolished activation of pro-migratory/pro-survival kinases (i.e FAK, Src, HSP27) triggered by osteoblasts, along with expression of matrix metalloproteinase-13. This suggests that CD151 participates in communication between PC3 cells and bone microenvironment and the process can be considered as a significant step of PCa progression and metastasis.
authors.affiliation: Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Grudowska
authors.affiliation: Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland.|Department of Cell Biology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Czaplińska
authors.affiliation: Department of Urology, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Polom
authors.affiliation: Department of Urology, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Matuszewski
authors.affiliation: Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Sądej
authors.affiliation: Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Andrzej C
authors.initials: AC
authors.lastname: Składanowski
mesh_terms: D000328:Adult; D001842:Bone and Bones / Q000473:pathology; D002450:Cell Communication; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement; D002470:Cell Survival; D005347:Fibroblasts / Q000473:pathology / Q000502:physiology; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D010006:Osteoblasts / Q000502:physiology*; D011471:Prostatic Neoplasms / Q000473:pathology / Q000503:physiopathology*; D060185:Tetraspanin 24 / Q000502:physiology*; D059470:Tetraspanins / Q000502:physiology
chemical_list: D060185:Tetraspanin 24; D059470:Tetraspanins
keywords: CD151; osteoblasts; prostate cancer