title: Enhancing the effect of 4MeV electron beam using gold nanoparticles in breast cancer cells.
abstract: Gold nanoparticles (GNPs) have been applied as radiosensitizer in radiotherapy. Limited reports have shown that GNPs may be effective as a dose enhancer agent for electron radiation therapy. Some Monte Carlo Simulation studies have shown that selecting suitable size of GNPs and electron energies are critical for effective dose enhancement. The aim of this study was to assess possible radiosensitization effect of GNPs on cancer cell treated with 4MeV electron beams. Approximately 10nm GNPs were synthesized and characterized by electron microscope and dynamic light scattering. MCF-7 and MDA-MB-231 breast cancer cells were used and their viability was measured by MTT assay. Radiosensitization effect of GNPs under 4MeV electron beams was measured by clonogenic assay. The result showed a concentration dependent uptake of GNPs without reducing cell viability at concentrations ≤50mg/L. Incubation of cancer cells with GNPs caused a significant decrease in their viability following exposure to electron beams as well as a decrease in their survival fraction when compared to control. The sensitizer enhancement ratio (SER) by electron beams in MCF-7 cells was 1.43 and 1.40 in presence of 25 and 50mg/L GNPs, respectively. For MDA-MB-231 cells, it was 1.62 in presence of 25mg/L GNPs. Our data demonstrated the significant dose enhancement of the GNPs in combination with 4MeV electron beams that could be applicable for the treatment of superficial tumors and intra operative radiation therapy.
authors.affiliation: Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Somayeh Sadat
authors.initials: SS
authors.lastname: Mehrnia
authors.affiliation: Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: bhashemi@modars.ac.ir.
authors.forename: Bijan
authors.initials: B
authors.lastname: Hashemi
authors.affiliation: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Mowla
authors.affiliation: Department of Radiotherapy, Imam Hossein (A.S.) Hospital, Shahid Beheshti University of Medical Science, Tehran, Iran.
authors.forename: Azim
authors.initials: A
authors.lastname: Arbabi
mesh_terms: D001943:Breast Neoplasms / Q000532:radiotherapy*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D000067493:Dynamic Light Scattering; D004583:Electrons / Q000627:therapeutic use*; D006046:Gold; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D008854:Microscopy, Electron; D011829:Radiation Dosage; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage* / Q000138:chemical synthesis
chemical_list: D011838:Radiation-Sensitizing Agents; D006046:Gold
keywords: Breast cancer; Electron beams; Gold nanoparticles; Irradiation; Radiosensitization