title: Fingerprint Analysis and Identification of Strains ST309 as a Potential High Risk Clone in aPseudomonas aeruginosaPopulation Isolated from Children with Bacteremia in Mexico City.
abstract: Pseudomonas aeruginosais an opportunistic pathogen and is associated with nosocomial infections. Its ability to thrive in a broad range of environments is due to a large and diverse genome of which its accessory genome is part. The objective of this study was to characterizeP. aeruginosastrains isolated from children who developed bacteremia, using pulse-field gel electrophoresis, and in terms of its genomic islands, virulence genes, multilocus sequence type, and antimicrobial susceptibility. Our results showed thatP. aeruginosastrains presented the seven virulence genes:toxA, lasB, lecA, algR, plcH, phzA1, andtoxR, a type IV pilin alleles (TFP) group I or II. Additionally, we detected a novel pilin and accessory gene, expanding the number of TFP alleles to group VI. All strains presented the PAPI-2 Island and the majority wereexoU+ andexoS+ genotype. Ten percent of the strains were multi-drug resistant phenotype, 18% extensively drug-resistant, 68% moderately resistant and only 3% were susceptible to all the antimicrobial tested. The most prevalent acquired β-Lactamase was KPC. We identified a group of ST309 strains, as a potential high risk clone. Our finding also showed that the strains isolated from patients with bacteremia have important virulence factors involved in colonization and dissemination as: a TFP group I or II; the presence of theexoUgene within the PAPI-2 island and the presence of theexoSgene.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México Mexico City, Mexico.
authors.forename: Rosario
authors.initials: R
authors.lastname: Morales-Espinosa
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México Mexico City, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Delgado
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México Mexico City, Mexico.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Espinosa
authors.affiliation: Servicio de Pediatría, Hospital Regional 36 San Alejandro, IMSS Puebla, Mexico.
authors.forename: Dassaev
authors.initials: D
authors.lastname: Isselo
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México Mexico City, Mexico.
authors.forename: José L
authors.initials: JL
authors.lastname: Méndez
authors.affiliation: Laboratorio de Bacteriología, Facultad de Veterinaria y Zootecnia, Universidad Nacional Autónoma de México Mexico City, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodriguez
authors.affiliation: Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Unidad de Investigación en Epidemiología Hospitalaria Mexico City, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Miranda
authors.affiliation: Global Evaluative Sciences USA, Inc. Seattle, WA, USA.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cravioto
keywords: Pseudomonas aeruginosa; ST309; TFP alleles; bacteremia; children; exoS and exoU genes; genomics island