title: Analysis of Ethylene Receptors: Ethylene-Binding Assays.
abstract: Plant ethylene receptors bind ethylene with high affinity. Most of the characterization of ethylene binding to the receptors has been carried out using a radioligand-binding assay on functional receptors expressed in yeast. In this chapter, we describe methods for expressing ethylene receptors in yeast and conducting ethylene-binding assays on intact yeast and yeast membranes. The ethylene-binding assays can be modified to analyze ethylene binding to intact plants and other organisms as well as membranes isolated from any biological source.
authors.affiliation: Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, M407 Walters Life Sciences, 1414 Cumberland Ave., Knoxville, TN, 37996, USA. bbinder@utk.edu.
authors.forename: Brad M
authors.initials: BM
authors.lastname: Binder
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, NH, 03755, USA.
authors.forename: G Eric
authors.initials: GE
authors.lastname: Schaller
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism*; D007553:Isotope Labeling; D008024:Ligands; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D005030:Ethylenes; D008024:Ligands; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant
keywords: Ethylene binding; Ethylene receptor; Radioligand; Yeast expression