title: Identification and differentiation of major components in three different "Sheng-ma" crude drug species by UPLC/Q-TOF-MS.
abstract: Cimicifugae Rhizoma (Sheng ma) is a Ranunculaceae herb belonging to a composite family and well known in China. has been widely used in traditional Chinese medicine. ThePharmacopoeia of the People׳s Republic of Chinacontains three varieties (Cimicifuga dahurica(Turcz.),Cimicifuga foetidaL. andCimicifuga heracleifoliaKom.) which have been used clinically as "Sheng-ma". However, the chemical constituents of three components of "Sheng-ma" have never been documented. In this study, a rapid method for the analysis of the main components of "Sheng-ma" was developed using ultra-high performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS). The present study reveals the major common and distinct chemical constituents ofC. dahurica,C. foetidaandC. heracleifoliaand also reports principal component and statistical analyses of these results. The components were identified by comparing the retention time, accurate mass, mass spectrometric fragmentation characteristic ions and matching empirical molecular formula with that of the published compounds. A total of 32 common components and 8 markers for different "Sheng-ma" components were identified. These findings provide an important basis for the further study and clinical utilities of the three "Sheng-ma" varieties.
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China; Nanjing Haichang Chinese Medicine Group Co. Ltd., Nanjing 210061, China.
authors.forename: Mengxue
authors.initials: M
authors.lastname: Fan
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China; Nanjing Haichang Chinese Medicine Group Co. Ltd., Nanjing 210061, China.
authors.forename: Kunming
authors.initials: K
authors.lastname: Qin
authors.affiliation: Nanjing Haichang Chinese Medicine Group Co. Ltd., Nanjing 210061, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China; Nanjing Haichang Chinese Medicine Group Co. Ltd., Nanjing 210061, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China; Nanjing Haichang Chinese Medicine Group Co. Ltd., Nanjing 210061, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China; Nanjing Haichang Chinese Medicine Group Co. Ltd., Nanjing 210061, China.
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
keywords: Chemical marker; Chemical profiling; Cimicifuga; Cimicifugae Rhizoma; Sheng-ma; UPLC/Q-TOF-MS