title: Fermentation and crystallization of succinic acid from Actinobacillus succinogenes ATCC55618 using fresh cassava root as the main substrate.
abstract: The fermentation of succinic acid from fresh cassava root using Actinobacillus succinogenes ATCC55618, and the recovery of the product using crystallization were investigated. Fresh cassava root is an ideal succinic acid feedstock due to its low price and high starch content. Saccharification was carried out using commercially available enzymes and diammonium phosphate was used as an inexpensive nitrogen source. Different fermentation modes were compared in terms of product yield and productivity. Results for fed-batch fermentations showed that a succinic acid titer of 151.44g/L, with yield and productivity of 1.51gSA/gglucoseand 3.22g/L/h could be obtained. Seeded batch cooling crystallization was investigated after pre-treatment using nanofiltration. A succinic acid crystal purity of 99.35% with a relative crystallinity of 96.77% was obtained from high seeding experiments. These results indicated that fresh cassava roots could be an economically alternative feedstock for a high quality succinic acid production.
authors.affiliation: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand.
authors.forename: Nguyen Thi Huong
authors.initials: NTH
authors.lastname: Thuy
authors.affiliation: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand.
authors.forename: Artit
authors.initials: A
authors.lastname: Kongkaew
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan District, Rayong 21210, Thailand.
authors.forename: Adrian
authors.initials: A
authors.lastname: Flood
authors.affiliation: School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand; Cassava Research Center, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand. Electronic address: apichat@sut.ac.th.
authors.forename: Apichat
authors.initials: A
authors.lastname: Boontawan
mesh_terms: D000188:Actinobacillus; D003460:Crystallization; D005285:Fermentation; D002365:Manihot; D019802:Succinic Acid
chemical_list: D019802:Succinic Acid
keywords: Actinobacillus succinogenes; Cassava; Crystallization; Fermentation; Succinic acid