title: Stress assessment using hair cortisol of kangaroos affected by the lumpy jaw disease.
abstract: The aim of this study was to objectively assess stress of kangaroos affected by lumpy jaw disease (LJD) using plasma and hair cortisol concentrations. The plasma and hair samples were collected from kangaroos with LJD and healthy controls. Collected hair samples were extracted with methanol after washing with isopropanol, following which they were processed with the cortisol enzyme immunoassay kit. The plasma cortisol concentration of LJD animals tended to be higher than that of the control. Ventral hair cortisol, but not dorsal hair, of LJD animals was significantly higher than that of the control. In conclusion, stress in kangaroos infected with LJD could be assessed by measuring ventral hair cortisol.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.|Itozu no mori Zoological Park, 4-1-8 Kamiitozu, Kokurakita, Kitakyushu, Fukuoka 803-0845, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Sotohira
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sano
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Chigusa
authors.initials: C
authors.lastname: Arai
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Asakawa
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D006197:Hair / Q000737:chemistry*; D006854:Hydrocortisone / Q000032:analysis* / Q000097:blood; D007571:Jaw Diseases / Q000175:diagnosis / Q000662:veterinary*; D007614:Macropodidae; D010019:Osteomyelitis / Q000175:diagnosis / Q000523:psychology / Q000662:veterinary*; D013312:Stress, Physiological
chemical_list: D006854:Hydrocortisone
keywords: hair cortisol; kangaroo; lumpy jaw disease; objectively assessment; stress