title: spalt is functionally conserved in Locusta and Drosophila to promote wing growth.
abstract: Locusta has strong fly wings to ensure its long distance migration, but the molecular mechanism that regulates the Locusta wing development is poorly understood. To address the developmental mechanism of the Locusta flying wing, we cloned the Dpp target gene spalt (sal) and analyzed its function in wing growth in the Locusta. The Locusta wing size is apparently reduced with vein defects when sal is interfered by injection of dsRNA, indicating that sal is required for locust wing growth and vein formation. This function is conserved during the Drosophila wing development. To better understand sal's function in wing growth, we then used Drosophila wing disc as a model for further study. We found that sal promotes cell proliferation in the whole wing disc via positive regulation of a microRNA bantam. Our results firstly unravel sal's function in the Locusta wing growth and confirm a highly conserved function of sal in Locusta and Drosophila.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Suning
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Wangpeng
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation; D003001:Cloning, Molecular; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D048188:Locusta migratoria / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D035683:MicroRNAs; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014921:Wings, Animal / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D035683:MicroRNAs; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins; D014157:Transcription Factors; C471146:bantam microRNA, Drosophila; C064196:dpp protein, Drosophila; C085793:salm protein, Drosophila