title: Spatial and quantitative datasets of the pancreatic β-cell mass distribution in lean and obese mice.
abstract: A detailed understanding of pancreatic β-cell mass distribution is a key element to fully appreciate the pathophysiology of models of diabetes and metabolic stress. Commonly, such assessments have been performed by stereological approaches that rely on the extrapolation of two-dimensional data and provide very limited topological information. We present ex vivo optical tomographic data sets of the full β-cell mass distribution in cohorts of obese ob/ob mice and their lean controls, together with information about individual islet β-cell volumes, their three-dimensional coordinates and shape throughout the volume of the pancreas between 4 and 52 weeks of age. These data sets offer the currently most comprehensive public record of the β-cell mass distribution in the mouse. As such, they may serve as a quantitative and topological reference for the planning of a variety of in vivo or ex vivo experiments including computational modelling and statistical analyses. By shedding light on intra- and inter-lobular variations in β-cell mass distribution, they further provide a powerful tool for the planning of stereological sampling assessments.
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, Umeå S-90187, Sweden.
authors.forename: Saba
authors.initials: S
authors.lastname: Parween
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, Umeå S-90187, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Eriksson
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, Umeå S-90187, Sweden.
authors.forename: Christoffer
authors.initials: C
authors.lastname: Nord
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, Umeå S-90187, Sweden.
authors.forename: Elena
authors.initials: E
authors.lastname: Kostromina
authors.affiliation: Umeå Centre for Molecular Medicine, Umeå University, Umeå S-90187, Sweden.
authors.forename: Ulf
authors.initials: U
authors.lastname: Ahlgren
mesh_terms: D000818:Animals; D007515:Islets of Langerhans / Q000000981:diagnostic imaging*; D051379:Mice; D008820:Mice, Obese; D013045:Species Specificity; D041622:Tomography, Optical