title: Live Cell Imaging Reveals the Relocation of dsRNA Binding Proteins Upon Viral Infection.
abstract: Viral infection triggers a range of plant responses such as the activation of the RNA interference (RNAi) pathway. The double-stranded RNA binding (DRB) proteins DRB3 and DRB4 are part of this pathway and aid in defending against DNA and RNA viruses, respectively. Using live cell imaging, we show that DRB2, DRB3, and DRB5 relocate from their uniform cytoplasmic distribution to concentrated accumulation in nascent viral replication complexes (VRC) that develop following cell invasion by viral RNA. Inactivation of the DRB3 gene in Arabidopsis by T-DNA insertion rendered these plants less able to repress RNA viral replication. We propose a model for the early stages of virus defense in which DRB2, DRB3, and DRB5 are invasion sensors that relocate to nascent VRC, where they bind to viral RNA and inhibit virus replication.
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Barton
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.|2 Institute of Molecular Biology, Mainz, Germany.
authors.forename: Elke F
authors.initials: EF
authors.lastname: Roovers
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.|3 Centre for AgriBioscience, Department of Animal, Plant and Soil Sciences, School of Life Sciences, La Trobe University, Victoria 3086.
authors.forename: Quentin
authors.identifier: 0000-0002-5142-7886
authors.initials: Q
authors.lastname: Gouil
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.|4 Universidade Federal do Rio Grande do Sul, RS, Brazil; and.
authors.forename: Guilherme C
authors.initials: GC
authors.lastname: da Fonseca
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Reis
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Craig
authors.initials: C
authors.lastname: Jackson
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Robyn L
authors.initials: RL
authors.lastname: Overall
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Fusaro
authors.affiliation: 1 University of Sydney, Sydney, NSW, 2006, Australia.|5 Queensland University of Technology, Brisbane, QLD, Australia.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Waterhouse
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D017799:Cucumovirus / Q000502:physiology; D054884:Host-Pathogen Interactions; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D010942:Plant Viruses / Q000145:classification / Q000502:physiology; D030821:Plants, Genetically Modified; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D059008:Time-Lapse Imaging / Q000379:methods; D017865:Tospovirus / Q000502:physiology; D017806:Tymovirus / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C561224:DRB2 protein, Arabidopsis; C581070:DRB3 protein, Arabidopsis; C581071:DRB5 protein, Arabidopsis; D008164:Luminescent Proteins; D016601:RNA-Binding Proteins