title: Root microbiota drive direct integration of phosphate stress and immunity.
abstract: Plants live in biogeochemically diverse soils with diverse microbiota. Plant organs associate intimately with a subset of these microbes, and the structure of the microbial community can be altered by soil nutrient content. Plant-associated microbes can compete with the plant and with each other for nutrients, but may also carry traits that increase the productivity of the plant. It is unknown how the plant immune system coordinates microbial recognition with nutritional cues during microbiome assembly. Here we establish that a genetic network controlling the phosphate stress response influences the structure of the root microbiome community, even under non-stress phosphate conditions. We define a molecular mechanism regulating coordination between nutrition and defence in the presence of a synthetic bacterial community. We further demonstrate that the master transcriptional regulators of phosphate stress response in Arabidopsis thaliana also directly repress defence, consistent with plant prioritization of nutritional stress over defence. Our work will further efforts to define and deploy useful microbes to enhance plant performance.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Castrillo
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Paulo José Pereira Lima
authors.initials: PJ
authors.lastname: Teixeira
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Sur Herrera
authors.initials: SH
authors.lastname: Paredes
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Theresa F
authors.initials: TF
authors.lastname: Law
authors.affiliation: Department of Plant Molecular Genetics, Centro Nacional de Biotecnología, CNB-CSIC, Darwin 3, 28049 Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: de Lorenzo
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Meghan E
authors.initials: ME
authors.lastname: Feltcher
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Omri M
authors.initials: OM
authors.lastname: Finkel
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Natalie W
authors.initials: NW
authors.lastname: Breakfield
authors.affiliation: Department of Genetics, University of North Carolina, Chapel Hill, North Carolina, USA.|Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Piotr
authors.initials: P
authors.lastname: Mieczkowski
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Department of Genetics, University of North Carolina, Chapel Hill, North Carolina, USA.|Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Corbin D
authors.initials: CD
authors.lastname: Jones
authors.affiliation: Department of Plant Molecular Genetics, Centro Nacional de Biotecnología, CNB-CSIC, Darwin 3, 28049 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Paz-Ares
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.|Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Dangl
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D064307:Microbiota / Q000276:immunology / Q000502:physiology*; D009154:Mutation; D010710:Phosphates / Q000378:metabolism*; D057865:Plant Immunity / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism* / Q000382:microbiology*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C514695:PHR1 protein, Arabidopsis; D010710:Phosphates; D014157:Transcription Factors