title: Identification of Optimal Reference Genes for Normalization of RT-qPCR Data in Cancerous and Non-Cancerous Tissues of Human Uterine Cervix.
abstract: This study aimed to identify the most stably expressed reference genes from a panel of 32 candidate genes for normalization of reverse transcription-quantitative real-time polymerase chain reaction data in cancerous and non-cancerous tissues of human uterine cervix. Overall, PUM1, YWHAZ, and RPLP0 were identified as the most stably expressed genes in paired cancerous and non-cancerous tissues. The results were further stratified by the state of malignancy of the tissues, histopathological type of the cancer, and the human papillomavirus-type.
authors.affiliation: a Human Genome Centre, School of Medical Sciences, Health Campus , Universiti Sains Malaysia , Kelantan , Malaysia.
authors.forename: Shing Cheng
authors.initials: SC
authors.lastname: Tan
authors.affiliation: b Department of Obstetrics and Gynecology , School of Medical Sciences, Health Campus, Universiti Sains Malaysia , Kelantan , Malaysia.
authors.forename: Mohd Pazudin
authors.initials: MP
authors.lastname: Ismail
authors.affiliation: c Department of Obstetrics and Gynecology , Hospital Sultan Ismail , Johor , Malaysia.
authors.forename: Daniel Roza
authors.initials: DR
authors.lastname: Duski
authors.affiliation: d Department of Pathology , School of Medical Sciences, Health Campus, Universiti Sains Malaysia , Kelantan , Malaysia.
authors.forename: Nor Hayati
authors.initials: NH
authors.lastname: Othman
authors.affiliation: e Department of Nuclear Medicine , Radiotherapy and Oncology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia , Kelantan , Malaysia.
authors.forename: Venkata Murali Krishna
authors.initials: VM
authors.lastname: Bhavaraju
authors.affiliation: a Human Genome Centre, School of Medical Sciences, Health Campus , Universiti Sains Malaysia , Kelantan , Malaysia.
authors.forename: Ravindran
authors.initials: R
authors.lastname: Ankathil
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D002584:Cervix Uteri / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000592:standards; D052162:Human papillomavirus 16 / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000592:standards*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D012015:Reference Standards; D059467:Transcriptome; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000821:virology
keywords: Endogenous control genes; gene expression; normalization; quantitative real-time PCR; reference genes; uterine cervical neoplasms