title: NEUROD2 RegulatesStim1Expression and Store-Operated Calcium Entry in Cortical Neurons.
abstract: Calcium signaling controls many key processes in neurons, including gene expression, axon guidance, and synaptic plasticity. In contrast to calcium influx through voltage- or neurotransmitter-gated channels, regulatory pathways that control store-operated calcium entry (SOCE) in neurons are poorly understood. Here, we report a transcriptional control ofStim1(stromal interaction molecule 1) gene, which is a major sensor of endoplasmic reticulum (ER) calcium levels and a regulator of SOCE. By using a genome-wide chromatin immunoprecipitation and sequencing approach in mice, we find that NEUROD2, a neurogenic transcription factor, binds to an intronic element within theStim1gene. We show that NEUROD2 limitsStim1expression in cortical neurons and consequently fine-tunes the SOCE response upon depletion of ER calcium. Our findings reveal a novel mechanism that regulates neuronal calcium homeostasis during cortical development.
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Gokhan
authors.identifier: 0000-0002-5875-5815
authors.initials: G
authors.lastname: Guner
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Gizem
authors.identifier: 0000-0002-6227-3501
authors.initials: G
authors.lastname: Guzelsoy
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Fatma Sadife
authors.identifier: 0000-0002-3388-3597
authors.initials: FS
authors.lastname: Isleyen
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Gulcan Semra
authors.identifier: 0000-0002-5855-8547
authors.initials: GS
authors.lastname: Sahin
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Akkaya
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Efil
authors.initials: E
authors.lastname: Bayam
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Eser Ilgin
authors.identifier: 0000-0001-9028-979X
authors.initials: EI
authors.lastname: Kotan
authors.affiliation: Physics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Alkan
authors.identifier: 0000-0002-9831-3632
authors.initials: A
authors.lastname: Kabakcioglu
authors.affiliation: Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
authors.forename: Gulayse
authors.identifier: 0000-0003-1352-990X
authors.initials: G
authors.lastname: Ince-Dunn
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism; D017124:Conserved Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D007438:Introns; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D009687:Nuclear Proteins; D011485:Protein Binding; D000071737:Stromal Interaction Molecule 1 / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002118:Calcium; D018797:Cell Cycle Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D000071737:Stromal Interaction Molecule 1; D014157:Transcription Factors; C560898:MCIDAS protein, human; C098995:Neurod2 protein, mouse; C100757:Stim1 protein, mouse
keywords: Neurod2; calcium; genomics; store-operated calcium entry; transcription factor