title: High-resolution phenotypic profiling of natural products-induced effects on the single-cell level.
abstract: Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.
authors.affiliation: Red Sea Research Center, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
authors.forename: Stephan
authors.initials: S
authors.lastname: Kremb
authors.affiliation: Red Sea Research Center, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
authors.forename: Christian R
authors.initials: CR
authors.lastname: Voolstra
mesh_terms: D001688:Biological Products / Q000009:adverse effects* / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects; D057166:High-Throughput Screening Assays; D006801:Humans; D010641:Phenotype; D059010:Single-Cell Analysis
chemical_list: D001688:Biological Products