title: Nanoparticle mediated PPARγ gene delivery on dental implants improves osseointegration via mitochondrial biogenesis in diabetes mellitus rat model.
abstract: Diabetes mellitus (DM) has a detrimental effect on osseointegration, stability and longevity of implants due to osteoporosis. In this study, PPARγ-loaded dental implants were investigated for the improvement of osseointegration and peri-implantitis. Chitosan gold nanoparticles conjugated with PPARγ cDNA were introduced on titanium mini-implant surfaces for PPARγ release to rat mandibular. DM-induced rat mandible showed structural changes such as decreased bone mass and increased inflammatory molecules, and diminution of PPARγ expression and bone formation molecules compared to normal rats. PPARγ induced bone formation via reduction of inflammatory molecules even under glucose oxidative stress. Furthermore, PPARγ strongly activated mitochondrial biogenesis and cell viability via p-AMK and Wnt/β-catenin signaling. Consequently, PPARγ gene delivery on regional dental implants contributed osseointegration, new bone formation and mineralization in DM-induced rats. This study demonstrates that PPARγ can be used as a therapeutic gene with dental implantation in diabetic patients since regional PPARγ expression enhances osseointegration and implant longevity.
authors.affiliation: Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Jeong-Seok
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Dental Materials, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Department of Preventive Dentistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Jae-Gyu
authors.initials: JG
authors.lastname: Jeon
authors.affiliation: Division of Advanced Materials Engineering, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Il-Song
authors.initials: IS
authors.lastname: Park
authors.affiliation: Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea. Electronic address: yihokn@chonbuk.ac.kr.
authors.forename: Ho-Keun
authors.initials: HK
authors.lastname: Yi
mesh_terms: D000818:Animals; D001846:Bone Development; D015921:Dental Implants; D003920:Diabetes Mellitus; D018014:Gene Transfer Techniques; D008334:Mandible; D053758:Nanoparticles; D001678:Organelle Biogenesis; D016348:Osseointegration; D010024:Osteoporosis / Q000150:complications; D047495:PPAR gamma / Q000235:genetics*; D051381:Rats; D014025:Titanium
chemical_list: D015921:Dental Implants; D047495:PPAR gamma; D014025:Titanium
keywords: AMPK/Wnt signal; Chitosan gold nanoparticles; Gene delivery; Implant longevity; Implant surface modification; Inflammation