title: Reductively Degradable Poly(2-hydroxyethyl methacrylate) Hydrogels with Oriented Porosity for Tissue Engineering Applications.
abstract: Degradable poly(2-hydroxyethyl methacrylate) hydrogels were prepared from a linear copolymer (Mw= 49 kDa) of 2-hydroxyethyl methacrylate (HEMA), 2-(acethylthio)ethyl methacrylate (ATEMA), and zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC). The deprotection of ATEMA thiol groups by triethylamine followed by their gentle oxidation with 2,2'-dithiodipyridine resulted in the formation of reductively degradable polymers with disulfide bridges. Finally, a hydrogel 3D structure with an oriented porosity was obtained by gelation of the polymer in the presence of needle-like sodium acetate crystals. The pore diameter and porosity of resulting poly(2-hydroxyethyl methacrylate-co-2-(acethylthio)ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] hydrogels varied between 59 and 65 μm and between 70 and 79.6 vol % according to Hg porosimetry, and complete degradation of these materials was reached in 86 days in 0.33 mmol solution of l-cysteine/L in phosphate buffer. The cross-linked P(HEMA-ATEMA-MPC) hydrogels were evaluated as a possible support for human mesenchymal stem cells (MSCs). No cytotoxicity was found for the un-cross-linked thiol-containing and protected P(HEMA-ATEMA-MPC) chains up to a concentration of 5 and 1 wt % in α-minimum essential medium, respectively.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Hana
authors.identifier: http://orcid.org/0000-0002-8334-4508
authors.initials: H
authors.lastname: Macková
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Plichta
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Hlídková
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Sedláček
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Rafal
authors.initials: R
authors.lastname: Konefal
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Zhansaya
authors.initials: Z
authors.lastname: Sadakbayeva
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Miroslava
authors.identifier: http://orcid.org/0000-0003-2684-9814
authors.initials: M
authors.lastname: Dušková-Smrčková
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Daniel
authors.identifier: http://orcid.org/0000-0002-6907-9701
authors.initials: D
authors.lastname: Horák
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic , Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Šárka
authors.initials: Š
authors.lastname: Kubinová
mesh_terms: D006801:Humans; D020100:Hydrogels; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D011102:Polyhydroxyethyl Methacrylate
keywords: 2-(acethylthio)ethyl methacrylate; 2-methacryloyloxyethyl phosphorylcholine; hydrogel; oriented porosity; poly(2-hydroxyethyl methacrylate); reductively degradable