title: The role of interleukin-1b and its antagonist (diacerein) in estradiol benzoate-induced endometrial hyperplasia and atypia in female rats.
abstract: Endometrial hyperplasia (EH) is a common gynecological condition and may progress to carcinoma. We investigated the effect of diacerein (DIA) on estradiol benzoate (EB)-induced EH and atypia. DIA (50 mg/kg/day) was administered orally to rats for 4 weeks, in the presence or absence of EH induced by intramuscular injection of EB (60 μg/100 g) three times per week for 4 weeks. We measured levels of serum total cholesterol, uterine tissue malondialdehyde (MDA), total nitrites (NOx), superoxide dismutase (SOD) activity, caspase-3, interleukin-1b (IL-1b) immunoexpressions, and histopathology. Results showed that EB-induced EH and atypia manifested by significant increase in serum total cholesterol with increase in MDA and NOxlevels. EB showed the typical histopathological changes of EH and atypia. In addition, there was reduction in SOD activity and decrease in caspase-3 immunoexpressions but increase in IL-1b immunoexpressions. DIA was able to reduce EB-induced pathological changes.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, El-Minia University, 61511, El-Minia, Egypt.
authors.forename: Marwa M M
authors.identifier: http://orcid.org/0000-0003-0216-5020
authors.initials: MMM
authors.lastname: Refaie
authors.affiliation: Department of Pathology, Faculty of Medicine, El-Minia University, 61511, El-Minia, Egypt.
authors.forename: Maram
authors.initials: M
authors.lastname: El-Hussieny
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D004714:Endometrial Hyperplasia / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000633:toxicity; D005260:Female; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000880:Anthraquinones; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; C074283:estradiol 3-benzoate; C025292:diacerein; D004958:Estradiol
keywords: caspase-3; diacerein; endometrial hyperplasia; estradiol benzoate; interleukin-1b