title: Calcium dependent regulation of protein ubiquitination - Interplay between E3 ligases and calcium binding proteins.
abstract: The ubiquitination status of proteins and intracellular calcium levels are two factors which keep changing inside any living cell. These two events appear to be independent of each other but recent experimental evidences show that ubiquitination of cellular proteins are influenced by calcium, Calmodulin, Calmodulin-dependent kinase II and other proteins of calcium dependent pathways. E3 ligases like Nedd4, SCF complex, APC, GP78 and ITCH are important regulators of calcium mediated processes. A bioinformatics analysis to inspect sequences and interacting partners of 242 candidate E3 ligases show the presence of calcium and/or Calmodulin binding motifs/domains within their sequences. Building a protein-protein interaction (PPI) network of human E3 ligase proteins identifies Ca2+related proteins as direct interacting partners of E3 ligases. Review of literature, analysis of E3 ligase sequences and their interactome suggests an interconnectivity between calcium signaling and the overall UPS system, especially emphasizing that a subset of E3 ligases have importance in physiological pathways modulated by calcium.
authors.affiliation: Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India.
authors.forename: Rukmini
authors.initials: R
authors.lastname: Mukherjee
authors.affiliation: Structural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Jadavpur, Kolkata 700032, India.
authors.forename: Aneesha
authors.initials: A
authors.lastname: Das
authors.affiliation: Structural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Jadavpur, Kolkata 700032, India. Electronic address: saikat@iicb.res.in.
authors.forename: Saikat
authors.initials: S
authors.lastname: Chakrabarti
authors.affiliation: Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India. Electronic address: oishee.chakrabarti@saha.ac.in.
authors.forename: Oishee
authors.initials: O
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D002147:Calmodulin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D060066:Protein Interaction Maps; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D002147:Calmodulin; D044767:Ubiquitin-Protein Ligases
keywords: Calcium; Calcium mediated regulation; Calmodulin; Ubiquitin E3 ligase