title: Lycium barbarum polysaccharide protects human keratinocytes against UVB-induced photo-damage.
abstract: Ultraviolet B (UVB) irradiation plays a key role in skin damage, which induces oxidative and inflammatory damages, thereby causing photoaging or photocarcinogenesis. Lycium barbarum polysaccharide (LBP), the most biologically active fraction of wolfberry, possesses significant antioxidative and anti-inflammatory effects on multiple tissues. In the present study, the photoprotective effects and potential underlying molecular mechanisms of LBP against UVB-induced photo-damage were investigated in immortalized human keratinocytes (HaCaT cells). The data indicated that pretreatment with LBP significantly attenuated UVB-induced decrease in cell viability, increase in ROS production and DNA damage. LBP also significantly suppressed UVB-induced p38 MAPK activation, and subsequently reversed caspase-3 activation and MMP-9 expression. Notably, LBP was found to induce Nrf2 nuclear translocation and increase the expression of Nrf2-dependent ARE target genes. Furthermore, the protective effects of LBP were abolished by siRNA-mediated Nrf2 silencing. These results showed that the antioxidant LBP could partially protect against UVB irradiation-induced photo-damage through activation of Nrf2/ARE pathway, thereby scavenging ROS and reducing DNA damage, and subsequently suppressing UVB-induced p38 MAP pathway. Thus, LBP can be potentially used for skincare against oxidative damage from environmental insults.
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Li
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Li
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.
authors.forename: Liqian
authors.initials: L
authors.lastname: Peng
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.|b Guangzhou First People's Hospital, Guangzhou Medical University , Guangzhou , China.
authors.forename: Na
authors.initials: N
authors.lastname: Jiang
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.|c The Fifth Affiliated Hospital of Guangzhou Medical University , Guangzhou , China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.
authors.forename: Erting
authors.initials: E
authors.lastname: Zhang
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.
authors.forename: Bihua
authors.initials: B
authors.lastname: Liang
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.
authors.forename: Runxiang
authors.initials: R
authors.lastname: Li
authors.affiliation: a Guangzhou Institute of Dermatology , Guangzhou , China.
authors.forename: Huilan
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D051267:NF-E2-Related Factor 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C500223:lycium barbarum polysaccharide
keywords: LBP; Nrf2; antioxidant; photoprotection; ultraviolet