title: Assessment of Immunomodulatory Activities and in vitro Toxicity of New Quinolone 7-ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo[3,4-h]quinoline-7-carboxylate.
abstract: Our previous studies on leukemia cells L1210 and cervical cancer HeLa cells revealed cytotoxic effects of the 7-ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo[3,4-h]quinoline-7-carboxylate (E2h), a new synthetically prepared quinolone derivative, toward selected cancer cell lines. The aim of the present study was to examine the cytotoxicity of E2h toward next cell lines and tissues; that is, human cancer HL-60 and A549 cells, human non-cancer fibroblast BHNF-1 cells, and reconstructed human epidermis tissues. Further we investigated the immunomodulatory activity of E2h on murine macrophage RAW 264.7 cells. Selenadiazoloquinolone E2h induced specific antiproliferative/cytotoxic activity against leukemia HL-60 cells and is the potent inducer of apoptotic cell death. Quinolone derivative demonstrated the immunomodulatory activities on RAW 264.7 cell line murine macrophages. The immunobiological studies revealed time- and concentration-dependent effective immunomodulation of pro- and anti-inflammatory cytokines' release and antiproliferative/cytotoxic effect following exposure of RAW 264.7 cells to E2h.
  
  
  BACKGROUND
  DMEM, Dulbecco's modified eagle medium; DMSO, Dimethylsulfoxide; EtBr, Ethidium bromide; PI, Propidium iodide; E2h, 7-ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo[3,4-h]quinoline-7-carboxylate.
authors.affiliation: a Institute of Biochemistry, Nutrition and Health Protection , Faculty of Chemical and Food Technology, Slovak University of Technology , Bratislava , Slovak Republic.
authors.forename: Soňa
authors.initials: S
authors.lastname: Jantová
authors.affiliation: b Institute of Chemistry, Center for Glycomics , Slovak Academy of Sciences , Bratislava , Slovak Republic.
authors.forename: Ema
authors.initials: E
authors.lastname: Paulovičová
authors.affiliation: b Institute of Chemistry, Center for Glycomics , Slovak Academy of Sciences , Bratislava , Slovak Republic.
authors.forename: Lucia
authors.initials: L
authors.lastname: Paulovičová
authors.affiliation: a Institute of Biochemistry, Nutrition and Health Protection , Faculty of Chemical and Food Technology, Slovak University of Technology , Bratislava , Slovak Republic.|c Institute of Experimental Pharmacology and Toxicology , Slovak Academy of Sciences , Bratislava , Slovak Republic.
authors.forename: Dominika
authors.initials: D
authors.lastname: Topoľská
authors.affiliation: d Institute of Management, Slovak University of Technology , Bratislava , Slovak Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pánik
authors.affiliation: e Institute of Organic Chemistry, Catalysis and Petrochemistry , Faculty of Chemical and Food Technology, Slovak University of Technology , Bratislava , Slovak Republic.
authors.forename: Viktor
authors.initials: V
authors.lastname: Milata
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000188:drug therapy*; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D018922:HL-60 Cells; D006801:Humans; D056747:Immunomodulation; D007938:Leukemia / Q000188:drug therapy*; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D016566:Organoselenium Compounds / Q000138:chemical synthesis / Q000494:pharmacology*; D015363:Quinolones / Q000138:chemical synthesis / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
chemical_list: C000613243:7-ethyl 9-ethyl-6-oxo-6,9-dihydro(1,2,5)selenadiazolo(3,4-h)quinoline-7-carboxylate; D000970:Antineoplastic Agents; D016566:Organoselenium Compounds; D015363:Quinolones
keywords: Anticancer; HL-60; apoptosis; cytokines; cytotoxicity; immunomodulation; proliferation; quinolones