title: Strain differences in arsenic-induced oxidative lesion via arsenic biomethylation between C57BL/6J and 129X1/SvJ mice.
abstract: Arsenic is a common environmental and occupational toxicant with dramatic species differences in its susceptibility and metabolism. Mouse strain variability may provide a better understanding of the arsenic pathological profile but is largely unknown. Here we investigated oxidative lesion induced by acute arsenic exposure in the two frequently used mouse strains C57BL/6J and 129X1/SvJ in classical gene targeting technique. A dose of 5 mg/kg body weight arsenic led to a significant alteration of blood glutathione towards oxidized redox potential and increased hepatic malondialdehyde content in C57BL/6J mice, but not in 129X1/SvJ mice. Hepatic antioxidant enzymes were induced by arsenic in transcription in both strains and many were higher in C57BL/6J than 129X1/SvJ mice. Arsenic profiles in the liver, blood and urine and transcription of genes encoding enzymes involved in arsenic biomethylation all indicate a higher arsenic methylation capacity, which contributes to a faster hepatic arsenic excretion, in 129X1/SvJ mice than C57BL/6J mice. Taken together, C57BL/6J mice are more susceptible to oxidative hepatic injury compared with 129X1/SvJ mice after acute arsenic exposure, which is closely associated with arsenic methylation pattern of the two strains.
authors.affiliation: Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, Liaoning, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, Liaoning, China.
authors.forename: Xiafang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, Liaoning, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, Liaoning, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fang
authors.affiliation: Environment and Non-communicable Disease Research Center, School of Public Health, China Medical University, Shenyang, Liaoning, P. R. China.
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Experimental Teaching Center, School of Public Health, China Medical University, Shenyang, Liaoning, P. R. China.
authors.forename: Lanyue
authors.initials: L
authors.lastname: Gao
authors.affiliation: Environment and Non-communicable Disease Research Center, School of Public Health, China Medical University, Shenyang, Liaoning, P. R. China.
authors.forename: Guifan
authors.initials: G
authors.lastname: Sun
authors.affiliation: Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, Liaoning, China.|Experimental Teaching Center, School of Public Health, China Medical University, Shenyang, Liaoning, P. R. China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Pi
authors.affiliation: Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, Liaoning, China.|Experimental Teaching Center, School of Public Health, China Medical University, Shenyang, Liaoning, P. R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000410:Alanine Transaminase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D020261:Arsenic Poisoning / Q000097:blood* / Q000473:pathology / Q000652:urine; D018053:Arsenites / Q000493:pharmacokinetics / Q000633:toxicity*; D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism; D001711:Biotransformation; D005786:Gene Expression Regulation / Q000187:drug effects*; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008745:Methylation; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017670:Sodium Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite; D008315:Malondialdehyde; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C560749:GCLM protein, mouse; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione