title: Cumene hydroperoxide induced changes in oxidation-reduction potential in fresh and frozen seminal ejaculates.
abstract: Oxidation-reduction potential (ORP) is a newer integrated measure of the balance between total oxidants (reactive oxygen species-ROS) and reductants (antioxidants) that reflects oxidative stress in a biological system. This study measures ORP and evaluates the effect of exogenous induction of oxidative stress by cumene hydroperoxide (CH) on ORP in fresh and frozen semen using the MiOXSYS Analyzer. Semen samples from healthy donors (n = 20) were collected and evaluated for sperm parameters. All samples were then flash-frozen at -80°C. Oxidative stress was induced by CH (5 and 50 μmoles/ml). Static ORP (sORP-(mV/106sperm/ml) and capacity ORP (cORP-μC/106sperm/ml) were measured in all samples before and after freezing. All values are reported as mean ± SEM. Both 5 and 50 μmoles/ml of CH resulted in a significant decline in per cent motility compared to control in pre-freeze semen samples. The increase in both pre-freeze and post-thaw semen samples for sORP was higher in the controls than with 50 μmoles/ml of CH. The change from pre-freeze to post-thaw cORP was comparable. The system is a simple, sensitive and portable tool to measure the seminal ORP and its dynamic impact on sperm parameters in both fresh and frozen semen specimens.
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: A
authors.identifier: http://orcid.org/0000-0003-0585-1026
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: R
authors.identifier: http://orcid.org/0000-0002-9664-6978
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Department of Medical Bioscience, University of the Western Cape, Bellville, South Africa.
authors.forename: R
authors.identifier: http://orcid.org/0000-0003-1128-2982
authors.initials: R
authors.lastname: Henkel
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.|Department of Life Science and Bioinformatics, Assam University, Silchar, India.
authors.forename: S
authors.initials: S
authors.lastname: Roychoudhury
authors.affiliation: Tulane University Health Sciences Center, New Orleans, LA, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Sikka
authors.affiliation: Medical Physiology, Stellenbosch University, Tygerberg, South Africa.
authors.forename: S
authors.initials: S
authors.lastname: du Plessis
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Y B
authors.initials: YB
authors.lastname: Sarda
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: C
authors.initials: C
authors.lastname: Speyer
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: M
authors.initials: M
authors.lastname: Nouh
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: C
authors.initials: C
authors.lastname: Douglas
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Kayali
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: A
authors.initials: A
authors.lastname: Elshaer
authors.affiliation: Department of Urology, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: E
authors.initials: E
authors.lastname: Sabanegh
mesh_terms: D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods; D008297:Male; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D055101:Semen Analysis; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D016877:Oxidants; D017382:Reactive Oxygen Species; C007164:cumene hydroperoxide
keywords: antioxidants; cumene hydroperoxide; oxidation-reduction potential; oxidative stress; seminal ejaculate; sperm