title: Antileishmanial Activity ofHandroanthus serratifolius(Vahl) S. Grose (Bignoniaceae).
abstract: This study aimed to evaluate the leishmanicidal activity of ethanol extract, fractions, and isolated substance fromHandroanthus serratifoliusagainstLeishmania amazonensis. Furthermore, this activity was related to cytotoxicity, and the selectivity index was determined. The ethanol extract was obtained by maceration of the stem powder, and the extract was subjected to fractionation on chromatographic column. The lapachol was obtained by acid base extraction followed by purification in chromatographic column. The antipromastigote activity and cytotoxicity tests were carried out by the cell viability method (MTT). Modified THP-1 cells were infected withL. amazonensispromastigotes and treated for 24 h with different concentrations of the extract, fractions, and lapachol. The ethanol extract, dichloromethane, and ethyl acetate fractions were not active against promastigotes (IC50> 200μg/mL) or cytotoxic (CC50> 500μg/mL), and the selectivity index (SI) was greater than 2.5. The ethyl acetate fraction was active only in promastigotes; it is not cytotoxic (CC50> 500μg/mL, SI > 5). The lapachol was selectively active only against amastigote (IS > 2.5, CC50> 500μg/mL). In summary, lapachol and ethyl acetate fraction are promising against amastigote and promastigote forms, respectively.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, ICS, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: Erica Vanessa Souza
authors.initials: EV
authors.lastname: Costa
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, ICS, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: Heliton Patrick Cordovil
authors.initials: HP
authors.lastname: Brígido
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, ICS, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: João Victor da Silva E
authors.initials: JV
authors.lastname: Silva
authors.affiliation: Departamento de Botânica, Museu Paraense Emílio Goeldi, Belém, PA, Brazil.
authors.forename: Marlia Regina
authors.initials: MR
authors.lastname: Coelho-Ferreira
authors.affiliation: Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Geraldo Célio
authors.initials: GC
authors.lastname: Brandão
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, ICS, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: Maria Fâni
authors.identifier: 0000-0003-0804-5804
authors.initials: MF
authors.lastname: Dolabela