title: Protein synthesis in enuleated fertilized and unfertilized mouse eggs.
abstract: Fertilized and unfertilized C57BL/6J eggs were microsurgically enucleated and then analyzed for their capacity to synthesize proteins using 2-dimensional polyacrylamide gel electrophoresis. In both types of enucleated eggs (cytoplasts), protein synthesis continued and was still detected up to three days in culture. Shortly after enucleation, the pattern of polypeptides remained similar to the respective non-operated control eggs but it later became gradually reduced in intensity and complexity. After two days of culture the appearance of some new proteins typical for 2-cell embryos was observed in enucleated fertilized eggs only. Our findings suggest that maternal mRNA stored during oogenesis is utilized during the preimplantation period.
authors.affiliation: Department of Animal Biology, University of Geneva, 154 Rte de Malagnou, CH-1224, Geneva, Switzerland.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Petzoldt
authors.affiliation: The Jackson Laboratory, 04609, Bar Harbor, Maine, USA.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hoppe
authors.affiliation: Department of Animal Biology, University of Geneva, 154 Rte de Malagnou, CH-1224, Geneva, Switzerland.
authors.forename: Karl
authors.initials: K
authors.lastname: Illmensee
keywords: 2-Dimensional polyacrylamide gel electrophoresis; Maternal mRNA; Microsurgical enucleation; Mouse eggs; Protein synthesis