title: [Performance of GeneXpert MTB / RIF®in the diagnosis of extrapulmonary tuberculosis in Dakar: 2010-2015].
abstract: BACKGROUND
  The challenge facing developing countries is the availability of methods for rapid and accurate diagnosis of tuberculosis. Some molecular techniques offer this advantage, so we used GeneXpert MTB / RIF test in the diagnosis of extra-pulmonary tuberculosis to evaluate its performance compared with conventional methods.
  
  
  METHODS
  Between 2010 and 2015, 544 extrapulmonary clinical specimens were collected and analyzed by microscopy, culture and GeneXpert. The evaluation of antitubercular susceptibility testing was performed using the MGIT 960 system. Genotype MTBDRplus was used to confirm the cases of rifampicin resistance detected by the GX system.
  
  
  RESULTS
  The study population included 544 patients, 55.15% men and 44.85% women. Patients age ranged from 1-92 years with the majority in the 18-45 age group. The sensitivity and the overall specificity of microscopy was 43.86% and 98.36%, 94.74% and 97.95% for GeneXpert®respectively (95% CI). There were two discrepant rifampicin-resistant results between GeneXpert test and phenotypic method. Among these cases MTBDRplus test results showed 100% agreement with those of the MGIT 960.
  
  
  CONCLUSIONS
  This study shows that the GeneXpert test exhibits high sensitivity for routine diagnosis of extra-pulmonary tuberculosis and should be used instead of microscopy. The cases of rifampicin resistance detected by GeneXpert should be confirmed by other molecular testing methods before initiating treatment.
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal; Département des Sciences Biologiques, Université Cheikh Anta Diop, Dakar, Sénégal.
authors.forename: Awa Ba
authors.initials: AB
authors.lastname: Diallo
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Abdoulkader Issifi
authors.initials: AI
authors.lastname: Kollo
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal; Département des Sciences Biologiques, Université Cheikh Anta Diop, Dakar, Sénégal.
authors.forename: Makhtar
authors.initials: M
authors.lastname: Camara
authors.affiliation: UFR Sciences de la Santé, Université Gaston Berger, Saint Louis.
authors.forename: Seynabou
authors.initials: S
authors.lastname: Lo
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Gedeon Walbang
authors.initials: GW
authors.lastname: Ossoga
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Moustapha
authors.initials: M
authors.lastname: Mbow
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Farba
authors.initials: F
authors.lastname: Karam
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Mame Yacine Fall
authors.initials: MY
authors.lastname: Niang
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Aliou
authors.initials: A
authors.lastname: Thiam
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Awa Ndiaye
authors.initials: AN
authors.lastname: Diawara
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Souleymane
authors.initials: S
authors.lastname: Mboup
authors.affiliation: Laboratoire de Bactériologie, Virologie du CHNU Aristide Le Dantec, Sénégal.
authors.forename: Aissatou Gaye
authors.initials: AG
authors.lastname: Diallo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000995:Antitubercular Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D012189:Retrospective Studies; D012293:Rifampin / Q000494:pharmacology*; D012675:Senegal; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents; D012293:Rifampin
keywords: Dakar; GeneXpert MTB/RIF; extra-pulmonary tuberculosis