title: Effects of bone morphogenetic protein 4 (BMP4) on in vitro development and survival of bovine preantral follicles enclosed in fragments ovarian tissue.
abstract: The aim of this study was to evaluate the effects of different concentrations of BMP4 on activation, development and mRNA expression of GDF9, BMP15, PCNA, Bax and Bcl2 in cultured bovine follicles enclosed in ovarian tissues. Ovarian tissue fragments were cultured for 6 days in α-MEM+ alone or supplemented with different concentrations of BMP4 (10, 50 or 100 ng/ml). Classical histology was performed to analyze follicle growth and morphology, while real-time PCR was used to analyze mRNA levels in fresh and cultured tissues. After 6 days, the culture of ovarian tissue in α-MEM+ alone or supplemented with 10, 50 or 100 ng/ml BMP4 promoted follicular activation. The different concentrations of BMP4 maintained the percentage of normal follicles similar to results of the control. The presence of 100 ng/ml BMP-4 in culture medium increased oocyte and follicular diameters of primary and secondary follicles when compared with those follicles from uncultured control or cultured in α-MEM+ alone (P < 0.05). The tissues cultured in the presence of increasing concentrations of BMP4 had an increase in mRNA expression of the tested genes, but despite this the differences were not statistically significant. In conclusion, 100 ng/ml BMP4 promotes an increase in diameters of follicles and oocytes of primary and secondary follicles after 6 days of in vitro culture.
authors.affiliation: Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Sobral,CE,Brazil.
authors.forename: Ellen de Vasconcelos
authors.initials: EV
authors.lastname: da Cunha
authors.affiliation: Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Sobral,CE,Brazil.
authors.forename: Glaucinete Borges
authors.initials: GB
authors.lastname: de Souza
authors.affiliation: Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Sobral,CE,Brazil.
authors.forename: José Renato de Sousa
authors.initials: JRS
authors.lastname: Passos
authors.affiliation: Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Sobral,CE,Brazil.
authors.forename: Anderson Weiny Barbalho
authors.initials: AWB
authors.lastname: Silva
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction (BioRep),Federal University of Santa Maria (UFSM) Santa Maria,RS,Brazil.
authors.forename: Andressa Minussi
authors.initials: AM
authors.lastname: Dau
authors.affiliation: Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Sobral,CE,Brazil.
authors.forename: Márcia Viviane Alves
authors.initials: MVA
authors.lastname: Saraiva
authors.affiliation: Brazilian Agricultural Research Corporation (EMBRAPA) Goats and Sheep,Sobral,CE,Brazil.
authors.forename: Raimundo Nonato Braga
authors.initials: RNB
authors.lastname: Lobo
authors.affiliation: Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Av. Comandante Maurocélio Rocha Ponte 100,CEP 62041-040,Sobral,CE,Brazil.
authors.forename: José Roberto Viana
authors.initials: JRV
authors.lastname: Silva
mesh_terms: D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology*; D002417:Cattle; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D055429:Growth Differentiation Factor 9 / Q000235:genetics; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology*; D010053:Ovary / Q000166:cytology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D046509:Tissue Culture Techniques / Q000379:methods*; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D055430:Bone Morphogenetic Protein 15; D055415:Bone Morphogenetic Protein 4; D055429:Growth Differentiation Factor 9; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein
keywords: Activation; Cow; Culture; Ovary; Primordial follicle