title: Sex-Dependent Effects of HO-1 Deletion from Adipocytes in Mice.
abstract: Induction of heme oxygenase-1 (HO-1) has been demonstrated to decrease body weight and improve insulin sensitivity in several models of obesity in rodents. To further study the role of HO-1 in adipose tissue, we created an adipose-specific HO-1 knockout mouse model. Male and female mice were fed either a control or a high-fat diet for 30 weeks. Body weights were measured weekly and body composition, fasting blood glucose and insulin levels were determined every six weeks. Adipocyte-specific knockout of HO-1 had no significant effect on body weight in mice fed a high-fat diet but increased body weight in female mice fed a normal-fat diet. Although body weights were not different in females fed a high fat diet, loss of HO-1 in adipocytes resulted in significant alterations in body composition. Adipose-specific HO-1 knockout resulted in increased fasting hyperglycemia and insulinemia in female but not male mice on both diets. Adipose-specific knockout of HO-1 resulted in a significant loss of HO activity and a decrease in the protein levels of adiponectin in adipose tissue. These results demonstrate that loss of HO-1 in adipocytes has greater effects on body fat and fasting hyperglycemia in a sex-dependent fashion and that expression of HO-1 in adipose tissue may have a greater protective role in females as compared to males.
authors.affiliation: Department of Physiology & Biophysics, Center for Excellence in Cardiovascular-Renal Research, University of Mississippi Medical Center, 2500 North State St, Jackson, MS 39216, USA. hosickp@mail.montclair.edu.|Department of Exercise Science and Physical Education, Montclair State University, Montclair, NJ 07043, USA. hosickp@mail.montclair.edu.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Hosick
authors.affiliation: Department of Physiology & Biophysics, Center for Excellence in Cardiovascular-Renal Research, University of Mississippi Medical Center, 2500 North State St, Jackson, MS 39216, USA. mfweeks@crimson.ua.edu.
authors.forename: Mary Frances
authors.initials: MF
authors.lastname: Weeks
authors.affiliation: Department of Physiology & Biophysics, Center for Excellence in Cardiovascular-Renal Research, University of Mississippi Medical Center, 2500 North State St, Jackson, MS 39216, USA. mhankins@umc.edu.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Hankins
authors.affiliation: Department of Physiology & Biophysics, Center for Excellence in Cardiovascular-Renal Research, University of Mississippi Medical Center, 2500 North State St, Jackson, MS 39216, USA. kmoore4@umc.edu.
authors.forename: Kyle H
authors.initials: KH
authors.lastname: Moore
authors.affiliation: Department of Physiology & Biophysics, Center for Excellence in Cardiovascular-Renal Research, University of Mississippi Medical Center, 2500 North State St, Jackson, MS 39216, USA. dstec@umc.edu.
authors.forename: David E
authors.initials: DE
authors.lastname: Stec
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000483:Alleles; D000818:Animals; D015415:Biomarkers; D001786:Blood Glucose; D001823:Body Composition / Q000235:genetics; D001835:Body Weight; D059305:Diet, High-Fat; D004789:Enzyme Activation; D005215:Fasting; D005260:Female; D018390:Gene Targeting; D051547:Heme Oxygenase-1 / Q000172:deficiency* / Q000378:metabolism; D006946:Hyperinsulinism / Q000097:blood / Q000235:genetics; D018836:Inflammation Mediators / Q000378:metabolism; D007333:Insulin Resistance / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity; D009928:Organ Specificity / Q000235:genetics; D012737:Sex Factors
chemical_list: D052242:Adiponectin; D015415:Biomarkers; D001786:Blood Glucose; D018836:Inflammation Mediators; D051547:Heme Oxygenase-1
keywords: Cre recombinase; adiponectin; bilirubin; diabetes; insulin resistance; obesity