title: SHISA3Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma.
abstract: The purpose of this study was to evaluate the contribution ofSHISA3promoter methylation to laryngeal squamous cell carcinoma (LSCC).SHISA3promoter methylation status and expression were determined using methylation-specific polymerase chain reaction (MSP) and quantitative real-time PCR (qRT-PCR) in 93 paired LSCC and adjacent normal tissues, respectively. Furthermore, the regulatory function of theSHISA3promoter fragment was analyzed using a luciferase reporter assay. The results reveal that there is a significant increase inSHISA3methylation in LSCC tissues compared with corresponding nontumor tissues (P= 4.58E- 12). The qRT-PCR results show a significant association betweenSHISA3methylation and expression in LSCC (P= 1.67E- 03). In addition, the area under the receiver operating characteristic curve was 0.91. Consequently, a log-rank test and multivariate Cox analysis suggest thatSHISA3promoter hypermethylation is a predictor of poor overall survival for LSCC (log-rankP= 0.024; HR = 2.71; 95% CI = 1.024-7.177;P= 0.047). The results indicate thatSHISA3promoter hypermethylation might increase the risk of LSCC through regulation of gene expression and is a potential diagnostic and prognostic biomarker for LSCC.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Zhisen
authors.identifier: 0000-0001-6660-0488
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Chongchang
authors.initials: C
authors.lastname: Zhou
authors.affiliation: School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Jinyun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Ye
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Deng
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Cao
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Hao
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Lexi
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China.
authors.forename: Yuna
authors.identifier: 0000-0002-3417-0484
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D016147:Genes, Tumor Suppressor; D057809:HEK293 Cells; D006258:Head and Neck Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D008565:Membrane Proteins / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic; D016016:Proportional Hazards Models; D012372:ROC Curve; D000077195:Squamous Cell Carcinoma of Head and Neck; D015533:Transcriptional Activation
chemical_list: D014408:Biomarkers, Tumor; D008565:Membrane Proteins