title: Transgenic markers for mammalian chimeras.
abstract: We describe the construction of aggregation chimeras between normal and transgenic embryos containing multiple copies of mouse β-globin genes. The transgenic component of the chimeras is then detected in tissue sections by a DNA-DNA in situ hybridization technique, using a biotinylated DNA β-globin probe and an avidin-linked alkaline phosphatase detection system. The general advantages of transgenic markers for chimeras are discussed.
authors.affiliation: The Wistar Institute, 36th at Spruce Street, 19104, Philadelphia, PA, USA.
authors.forename: James Alexander
authors.initials: JA
authors.lastname: Thomson
authors.affiliation: The Wistar Institute, 36th at Spruce Street, 19104, Philadelphia, PA, USA.
authors.forename: Davor
authors.initials: D
authors.lastname: Solter
keywords: Chimeras; In situ hybridization; Mice; Transgenic