title: Serum microRNA profiling in patients with glioblastoma: a survival analysis.
abstract: Because circulating microRNAs (miRNAs) have drawn a great deal of attention as promising novel cancer diagnostics and prognostic biomarkers, we sought to identify serum miRNAs significantly associated with outcome in glioblastoma patients. To do this, we performed global miRNA profiling in serum samples from 106 primary glioblastoma patients. The study subjects were randomly divided into two sets: set one (n = 40) and set two (n = 66). Using a Cox regression model, 3 serum miRNAs (miR-106a-5p, miR-182, and miR-145-5p) and 5 serum miRNAs (miR-222-3p, miR-182, miR-20a-5p, miR-106a-5p, and miR-145-5p) were identified significantly associated with 2-year patient overall survival and disease-free survival (P < 0.05) in both sets and the combined set. We then created the miRNA risk scores to assess the total impact of the significant serum miRNAs on survival. The high risk scores were associated with poor patient survival (overall survival: HR = 1.92, 95% CI: 1.19, 10.23, and disease-free survival: HR = 2.03, 95%CI: 1.24, 4.28), and were independent of other clinicopathological factors. Our results suggest that serum miRNAs could serve as prognostic predictors of glioblastoma.
authors.affiliation: Department of Epidemiology, The University of Texas MD Anderson Cancer Center, 1155 Pressler Street, Houston, TX, 77030, USA. hzhao2@mdanderson.org.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Epidemiology, The University of Texas MD Anderson Cancer Center, 1155 Pressler Street, Houston, TX, 77030, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Neuro-Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
authors.forename: Tiffany R
authors.initials: TR
authors.lastname: Hodges
authors.affiliation: Department of Epidemiology, The University of Texas MD Anderson Cancer Center, 1155 Pressler Street, Houston, TX, 77030, USA.
authors.forename: Renduo
authors.initials: R
authors.lastname: Song
authors.affiliation: Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
authors.forename: Gregory N
authors.initials: GN
authors.lastname: Fuller
authors.affiliation: Department of Neuro-Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
authors.forename: Amy B
authors.initials: AB
authors.lastname: Heimberger
mesh_terms: D014408:Biomarkers, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000097:blood / Q000235:genetics* / Q000401:mortality; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Glioblastoma; Serum microRNAs; and prognosis