title: Selenium Polysaccharide SPMP-2a fromPleurotus geesteranusAlleviates H2O2-Induced Oxidative Damage in HaCaT Cells.
abstract: Selenium- (Se-) enriched polysaccharide SPMP-2a was extracted and purified fromPleurotus geesteranus. SPMP-2a is a white flocculent polysaccharide and soluble in water, with a molecular weight of 3.32 × 104Da. Fourier transform infrared spectroscopy spectral analysis indicated that it belongs to an acid Se polysaccharide withα-D-glucopyranoside bond. The effects of Se polysaccharide SPMP-2a inP. geesteranusagainst hydrogen peroxide- (H2O2-) induced oxidative damage in human keratinocytes (HaCaT) cells were evaluated further. Reduced cell viability and elevated apoptotic rates in H2O2-treated HaCaT cells were proven by MTT and flow cytometry assays. Hoechst 33342 staining revealed chromatin condensations in the nuclei of HaCaT cells. However, with the addition of SPMP-2a, cell viability improved, nuclear condensation declined, and cell apoptotic rates dropped significantly. Ultrastructural observation consistently revealed that treatments with SPMP-2a reduced the number of swollen and vacuolar mitochondria in the H2O2-treated cells compared with the controls. Furthermore, SPMP-2a increased the superoxide dismutase (SOD) and catalase (CAT) activities and reduced reactive oxygen species (ROS) content. Western blot analysis showed that SPMP-2a treatment effectively increased B-cell lymphoma 2 (Bcl-2) protein expression. Therefore, SPMP-2a could improve cellular antioxidant enzyme activities, reduce ROS levels, and increase Bcl-2 protein expression levels, thereby reducing cell apoptosis and protecting HaCaT cells from H2O2-induced oxidative damage.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei 230036, China; School of Life Science, Anhui Science and Technology University, Fengyang 233100, China.
authors.forename: Yujun
authors.identifier: 0000-0002-9034-2674
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Life Science, Anhui Science and Technology University, Fengyang 233100, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhou
authors.affiliation: School of Life Science, Anhui Science and Technology University, Fengyang 233100, China.
authors.forename: Shoucheng
authors.initials: S
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Changjun
authors.identifier: 0000-0002-4097-8295
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D017209:Apoptosis; D002374:Catalase / Q000378:metabolism; D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008970:Molecular Weight; D016566:Organoselenium Compounds / Q000737:chemistry*; D018384:Oxidative Stress; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; C000595707:BCL2 protein, human; D016566:Organoselenium Compounds; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D012643:Selenium