title: Protective Effect of Pistacia lentiscus Oil Against Bleomycin-Induced Lung Fibrosis and Oxidative Stress in Rat.
abstract: We aimed in the present study to investigate the protective effect of Pistacia lentiscus oil against bleomycin-induced lung fibrosis as well as the involvement of oxidative stress in such protection. In this respect, adult male Wistar rats were used and divided into three groups of twenty each: control (NaCl, 0.9%), bleomycin, and bleomycin (4 mg/kg b.w.) + P. lentiscus oil (3 g/kg, b.w.). Animals were pretreated for 30 days before the induction of fibrosis by bleomycin and 1 wk after the induction of fibrosis. The oil principal compounds detected by gas chromatography analysis are: Linoleic and palmitic acids (70.6 and 24.7%, respectively). Our data demonstrated that P. lentiscus oil protected against bleomycin-induced fibrosis as evidenced by TGFβ immunostaining increase in lungs fibrocytes as well as inflammatory infiltrate. We also showed that acute bleomycin-induced fibrosis was accompanied by an oxidative stress in lung tissue as assessed by an increase of lipid peroxidation as well as antioxidant enzyme activities depletion such as superoxide dismutase (SOD) and catalase (CAT). More importantly, P. lentiscus oil treatment reversed all bleomycin-induced oxidative stress parameters disturbances. In conclusion, we suggest that P. lentiscus oil had potent protective effects against bleomycin-induced fibrosis due in part to its antioxidant properties.
authors.affiliation: a Unit Research 03/UR/08-05, Pulmonary Fibrosis: Prevention & Treatment, Faculty of Medicine of Tunis , Tunis , Tunisia.
authors.forename: Anouar
authors.initials: A
authors.lastname: Abidi
authors.affiliation: b Laboratory of Functional Physiology and Valorization of Bioresources , High Institute of Biotechnology of Beja, University of Jendouba , Beja , Tunisia.
authors.forename: Nadhem
authors.initials: N
authors.lastname: Aissani
authors.affiliation: b Laboratory of Functional Physiology and Valorization of Bioresources , High Institute of Biotechnology of Beja, University of Jendouba , Beja , Tunisia.
authors.forename: Hichem
authors.initials: H
authors.lastname: Sebai
authors.affiliation: c National School of Health Sciences of Tunis , Tunis , Tunisia.
authors.forename: Raja
authors.initials: R
authors.lastname: Serairi
authors.affiliation: d Department of Anatomy and Pathology , Charles Nicolle Hospital , Tunis , Tunisia.
authors.forename: Nadia
authors.initials: N
authors.lastname: Kourda
authors.affiliation: a Unit Research 03/UR/08-05, Pulmonary Fibrosis: Prevention & Treatment, Faculty of Medicine of Tunis , Tunis , Tunisia.
authors.forename: Saloua
authors.initials: S
authors.lastname: Ben Khamsa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001761:Bleomycin; D002374:Catalase / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D027927:Pistacia / Q000737:chemistry*; D010938:Plant Oils / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000188:drug therapy*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D010938:Plant Oils; D020011:Protective Agents; D001761:Bleomycin; D002374:Catalase; D013482:Superoxide Dismutase