title: Construction of Small-Diameter Vascular Graft by Shape-Memory and Self-Rolling Bacterial Cellulose Membrane.
abstract: Bacterial cellulose (BC) membranes with shape-memory properties allow the rapid preparation of artificial small-diameter blood vessels when combined with microfluidics-based patterning with multiple types of cells. Lyophilization of a wet multilayered rolled BC tube endows it with memory to recover its tubular shape after unrolling. The unrolling of the BC tube yields a flat membrane, and subsequent patterning with endothelial cells, smooth muscle cells, and fibroblast cells is carried out by microfluidics. The cell-laden BC membrane is then rerolled into a multilayered tube. The different cells constituting multiple layers on the tubular wall can imitate blood vessels in vitro. The BC tubes (2 mm) without cell modification, when implanted into the carotid artery of a rabbit, maintain thrombus-free patency 21 d after implantation. This study provides a novel strategy for the rapid construction of multilayered small-diameter BC tubes which may be further developed for potential applications as artificial blood vessels.
authors.affiliation: CAS Center of Excellence for Nanoscience, Beijing Engineering Research Center for BioNanotechnology for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China.|National Engineering Research Center for Nano-Medicine, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute and Hospital of Hepatobiliary Surgery, Key Laboratory of Digital Hepatobiliary Surgery of Chinese PLA, Chinese PLA Medical School, Chinese PLA General Hospital, Beijing, 100190, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Institute and Hospital of Hepatobiliary Surgery, Key Laboratory of Digital Hepatobiliary Surgery of Chinese PLA, Chinese PLA Medical School, Chinese PLA General Hospital, Beijing, 100190, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.affiliation: Institute and Hospital of Hepatobiliary Surgery, Key Laboratory of Digital Hepatobiliary Surgery of Chinese PLA, Chinese PLA Medical School, Chinese PLA General Hospital, Beijing, 100190, China.
authors.forename: Jinzhe
authors.initials: J
authors.lastname: Liu
authors.affiliation: CAS Center of Excellence for Nanoscience, Beijing Engineering Research Center for BioNanotechnology for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: College of Chemical Engineering, Qingdao University, Qingdao, 266071, China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: CAS Center of Excellence for Nanoscience, Beijing Engineering Research Center for BioNanotechnology for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China.|National Engineering Research Center for Nano-Medicine, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Junchuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: CAS Center of Excellence for Nanoscience, Beijing Engineering Research Center for BioNanotechnology for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China.
authors.forename: Zhaohe
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Ecole Normale Supérieure, 24 rue Lhomond, Paris, 75231, France.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: CAS Center of Excellence for Nanoscience, Beijing Engineering Research Center for BioNanotechnology for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China.
authors.forename: Nuoxin
authors.initials: N
authors.lastname: Wang
authors.affiliation: Institute and Hospital of Hepatobiliary Surgery, Key Laboratory of Digital Hepatobiliary Surgery of Chinese PLA, Chinese PLA Medical School, Chinese PLA General Hospital, Beijing, 100190, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: CAS Center of Excellence for Nanoscience, Beijing Engineering Research Center for BioNanotechnology for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China.
authors.forename: Wenfu
authors.initials: W
authors.lastname: Zheng
authors.affiliation: National Engineering Research Center for Nano-Medicine, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: CAS Center of Excellence for Nanoscience, Beijing Engineering Research Center for BioNanotechnology for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D002339:Carotid Arteries / Q000166:cytology / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry*; D041822:Gluconacetobacter xylinus / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008567:Membranes, Artificial; D011817:Rabbits
chemical_list: D008567:Membranes, Artificial; D002482:Cellulose
keywords: artificial blood vessel; bacterial cellulose; self-rolling; shape-memory