title: Antibiotic and Desiccation Resistance ofCronobacter sakazakiiandC. malonaticusIsolates from Powdered Infant Formula and Processing Environments.
abstract: This study evaluated the antimicrobial and desiccation resistance ofCronobacter sakazakiiandCronobacter malonaticusisolates from powdered infant formula and processing environments. The antimicrobial susceptibility tests showed that the 70Cronobacterstrains, representing 19 sequence types, were susceptible to the most of the antibiotics except for amoxicillin-clavulanate, ampicillin, and cefazolin. Furthermore, the growth of sixC. sakazakiiand twoC. malonaticusstrains from different sequence types (STs) in hyperosmotic media was measured. The growth of the twoC. sakazakiistrains (CE1 and CE13) from the neonatal pathovars ST4 and ST8, were significantly higher (p< 0.05) than that of other strains.C. malonaticusstrain CM35 (ST201) was the slowest grower in all strains, and most could not grow in more than 8% NaCl solution. Also the survival of these strains under desiccation conditions was followed for 1 year. The viable count ofCronobacterspp. under desiccation conditions was reduced on average by 3.02 log cycles during 1 year, with CE13 (ST8) being the most desiccation resistant strain. These results will improve our understanding of the persistence of the two closely related speciesC. sakazakiiandC. malonaticuswhich are of concern for neonatal and adult health.
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University Harbin, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Fei
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityHarbin, China; National Research Center of Dairy Engineering and Technology, Northeast Agricultural UniversityHarbin, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University Harbin, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.affiliation: Pathogen Research Group, School of Science and Technology, Nottingham Trent University Nottingham, UK.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Forsythe
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University Harbin, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University Harbin, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University Harbin, China.
authors.forename: Chaoxin
authors.initials: C
authors.lastname: Man
keywords: C. malonaticus; C. sakazakii; antibiotic susceptibility; desiccation; powdered infant formula