title: Brachypodium distachyonBdPP2CA6 Interacts with BdPYLs and BdSnRK2 and Positively Regulates Salt Tolerance in TransgenicArabidopsis.
abstract: The phytohormone abscisic acid (ABA) is essential in plant responding to biotic and abiotic stresses. Although ABA signaling model is well established inArabidopsis, ABA receptor PYL family and clade A PP2C subfamily are not yet characterized in monocot model plantBrachypodium distachyon. In this study, we identified 12PYLsand 8 clade APP2CsfromB. distachyongenome and successfully cloned 12PYLsand 7 clade APP2Cs. Bioinformatic and expression analyses showed that most of the identified genes respond to several signal molecules and abiotic stresses. Protein-protein interaction analysis revealed that many BdPYLs and BdPP2CAs participate in the classic ABA-PYL-PP2C-SnRK2 signaling pathway. A clade A PP2C, designated BdPP2CA6, interacted with BdPYL11 in the absence of ABA and localized in nucleus. Most clade A PP2C members fromArabidopsisshowed negatively regulation in ABA signaling pathway, whereasBdPP2CA6-overexpression transgenicArabidopsisshowed ABA hypersensitive phenotype, resulting in enhanced stomatal closure and salinity tolerance. Our results indicate thatBdPP2CA6positively regulates ABA and stress signal pathway in transgenicArabidopsisplant seedlings.
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Wei
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Jiaochun
authors.initials: J
authors.lastname: Shi
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Jin
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Qingchen
authors.initials: Q
authors.lastname: Luo
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Chang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Guangxiao
authors.initials: G
authors.lastname: Yang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: He
keywords: ABA; Brachypodium distachyon; PP2C; abiotic stress; salinity