title: Transmission of cytokinesis forces via E-cadherin dilution and actomyosin flows.
abstract: During epithelial cytokinesis, the remodelling of adhesive cell-cell contacts between the dividing cell and its neighbours has profound implications for the integrity, arrangement and morphogenesis of proliferative tissues. In both vertebrates and invertebrates, this remodelling requires the activity of non-muscle myosin II (MyoII) in the interphasic cells neighbouring the dividing cell. However, the mechanisms that coordinate cytokinesis and MyoII activity in the neighbours are unknown. Here we show that in the Drosophila notum epithelium, each cell division is associated with a mechanosensing and transmission event that controls MyoII dynamics in neighbouring cells. We find that the ring pulling forces promote local junction elongation, which results in local E-cadherin dilution at the ingressing adherens junction. In turn, the reduction in E-cadherin concentration and the contractility of the neighbouring cells promote self-organized actomyosin flows, ultimately leading to accumulation of MyoII at the base of the ingressing junction. Although force transduction has been extensively studied in the context of adherens junction reinforcement to stabilize adhesive cell-cell contacts, we propose an alternative mechanosensing mechanism that coordinates actomyosin dynamics between epithelial cells and sustains the remodelling of the adherens junction in response to mechanical forces.
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.|Graduate Program in Areas of Basic and Applied Biology, Abel Salazar Biomedical Sciences Institute, University of Porto, Portugal.
authors.forename: Diana
authors.initials: D
authors.lastname: Pinheiro
authors.affiliation: Cavendish Laboratory, Department of Physics, J. J. Thomson Avenue, University of Cambridge, Cambridge CB3 0HE, UK.|The Wellcome Trust/Cancer Research UK Gurdon Institute, Tennis Court Rd, University of Cambridge, Cambridge CB2 1QN, UK.
authors.forename: Edouard
authors.initials: E
authors.lastname: Hannezo
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Herszterg
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Floris
authors.initials: F
authors.lastname: Bosveld
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Gaugue
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Balakireva
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Inês
authors.initials: I
authors.lastname: Cristo
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Stéphane U
authors.initials: SU
authors.lastname: Rigaud
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Olga
authors.initials: O
authors.lastname: Markova
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, France.
authors.forename: Yohanns
authors.initials: Y
authors.lastname: Bellaïche
mesh_terms: D000205:Actomyosin / Q000378:metabolism*; D022005:Adherens Junctions / Q000378:metabolism*; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D002455:Cell Division; D048749:Cytokinesis; D004331:Drosophila melanogaster / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D024462:Myosin Type II / Q000378:metabolism*
chemical_list: D015820:Cadherins; D000205:Actomyosin; D024462:Myosin Type II