title: Hot-Water Extracts from Roots of Vitis thunbergii var. taiwaniana and Identified ε-Viniferin Improve Obesity in High-Fat Diet-Induced Mice.
abstract: In this study, hot-water extracts (HW) from roots of Vitis thunbergii var. taiwaniana (VTT-R) were shown to lower levels of lipid accumulation significantly (P < 0.01 or 0.001) compared to the control in 3T3-L1 adipocytes. The VTT-R-HW (40 mg/kg) interventions concurrent with a high-fat (HF) diet in C57BL/6 mice over a 5 eek period were shown to reduce body weights significantly (P < 0.05) compared to those of mice fed a HF diet under the same food-intake regimen. The (+)-ε-viniferin isolated from VTT-R-HW was shown to reduce the size of lipid deposits significantly compared to the control (P < 0.05 or 0.001) in 3T3-L1 adipocytes, and dose-dependent 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitions showed that the 50% inhibitory concentration was calculated to be 96 μM. The two-stage (+)-ε-viniferin interventions (10 mg/kg, day 1 to day 38; 25 mg/kg, day 39 to day 58) were shown to lower mice body weights significantly (P < 0.05 or 0.001), the weight ratio of mesenteric fat, blood glucose, total cholesterol, and low-density lipoprotein compared to that of the HF group under the same food-intake regimen but without concurrent VTT-R-HW interventions. It might be possible to use VTT-R-HW or (+)-ε-viniferin as an ingredient in the development of functional foods for weight management, and this will need to be investigated further.
authors.forename: Yeh-Lin
authors.initials: YL
authors.lastname: Lu
authors.forename: Shyr-Yi
authors.initials: SY
authors.lastname: Lin
authors.forename: Sheng-Uei
authors.initials: SU
authors.lastname: Fang
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Hsieh
authors.affiliation: Department of Life Science, National Taitung University , Taitung 950, Taiwan.
authors.forename: Chiy-Rong
authors.initials: CR
authors.lastname: Chen
authors.affiliation: Taiwan Seed Improvement and Propagation Station, Council of Agriculture , Taichung 426, Taiwan.
authors.forename: Chi-Luan
authors.initials: CL
authors.lastname: Wen
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology , Pingtung 912, Taiwan.
authors.forename: Chi-I
authors.initials: CI
authors.lastname: Chang
authors.forename: Wen-Chi
authors.identifier: http://orcid.org/0000-0002-9565-7018
authors.initials: WC
authors.lastname: Hou
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D013267:Stilbenes / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D027843:Vitis / Q000737:chemistry*
chemical_list: D001572:Benzofurans; D001786:Blood Glucose; D010936:Plant Extracts; D013267:Stilbenes; C091891:epsilon-viniferin; D002784:Cholesterol
keywords: (+)-ε-viniferin; 3T3-L1 adipocyte; Vitis thunbergii var. taiwaniana; high-fat (HF) diet; obesity