title: Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction.
abstract: Matrix Metalloproteinases (MMPs) are a family of proteolytic enzymes whose endopeptidase activity is dependent on the presence of specific metal ions. MT1-MMP (or MMP-14), which has been implicated in tumor progression and cellular invasion, contains a membrane-spanning region located C-terminal to a hemopexin-like domain and an N-terminal catalytic domain. We recombinantly expressed the catalytic domain of human MT1-MMP in E. coli and purified it from inclusion bodies using a refolding protocol that yielded significant quantities of active protein. Crystals of MT1-MMP were obtained using the vapour diffusion method. Here, we describe the protocols used for crystallization and the data analysis together with the resulting diffraction pattern.
authors.affiliation: Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, 45470, Mülheim an der Ruhr, Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Decaneto
authors.affiliation: Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, 45470, Mülheim an der Ruhr, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lubitz
authors.affiliation: Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, 45470, Mülheim an der Ruhr, Germany. hideaki.ogata@cec.mpg.de.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ogata
mesh_terms: D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D053511:Matrix Metalloproteinase 14 / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D011487:Protein Conformation; D015202:Protein Engineering; D058849:Protein Refolding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: Crystallization; Diffraction; MMP-14; MT1-MMP; Metalloproteinase