title: The association between Nd:YAG laser and desensitizing dentifrices for the treatment of dentin hypersensitivity.
abstract: This study aims to evaluate the association between Nd:YAG laser (with and without a photoabsorber) and two desensitizing dentifrices containing 15% NovaMin or 8% arginine, as potential treatments for dentin hypersensitivity (DH). DH was simulated by EDTA application for 2 min. Specimens were then analyzed with an environmental scanning electron microscope (ESEM) to ensure open dentin tubules (ODT), counted by using ImageJ software. Specimens were randomized into eight groups (n = 10): Laser (L), Laser+Photoabsorber (LP), Arginine (A), Arginine+Laser (AL), Arginine+Laser+Photoabsorber (ALP), NovaMin (N), NovaMin+Laser (NL), and NovaMin+Laser+Photoabsorber (NLP). Laser irradiation was performed with 1 W, 100 mJ, 10 Hz, ≅85 J/cm2; 4 irradiations of 10 s each, with 10 s intervals between them. After treatment, specimens were again analyzed by ESEM and submitted to erosive/abrasive cycling for 5 days. A final ESEM analysis was performed. Data were analyzed with two-way repeated measure ANOVA and Tukey tests (α = 0.05). After treatment, groups N, NL, and NLP presented the lower number of ODT, but they did not different from LP, ALP, and AL. Group A presented the highest number of ODT and it did not differ from group L. Groups L, AL, ALP, and LP presented intermediate results, without differing from each other. After cycling, group A presented the highest number of ODT and did not differ significantly from the other groups, except NLP. None of the associations tested presented better tubule occlusion than NovaMin by itself. Arginine was the only treatment that presented improved tubule occlusion when associated with Nd:YAG laser.
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Professor Lineu Prestes 2227, São Paulo, SP, Brazil.
authors.forename: Sandra Ribeiro
authors.initials: SR
authors.lastname: Cunha
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Professor Lineu Prestes 2227, São Paulo, SP, Brazil.
authors.forename: Stephanie Assimakopoulos
authors.initials: SA
authors.lastname: Garófalo
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Professor Lineu Prestes 2227, São Paulo, SP, Brazil.
authors.forename: Taís
authors.initials: T
authors.lastname: Scaramucci
authors.affiliation: Institute of Nuclear Research (IPEN), University of São Paulo - IPEN/CNEN, Av. Professor Lineu Prestes 2242, São Paulo, SP, Brazil.
authors.forename: Denise Maria
authors.initials: DM
authors.lastname: Zezell
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Professor Lineu Prestes 2227, São Paulo, SP, Brazil. acca@usp.br.|Special Laboratory of Lasers in Dentistry, Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes 2227, 05508-000, São Paulo, SP, Brazil. acca@usp.br.
authors.forename: Ana Cecilia Corrêa
authors.initials: ACC
authors.lastname: Aranha
mesh_terms: D003802:Dentifrices / Q000528:radiation effects*; D003804:Dentin / Q000528:radiation effects / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000494:pharmacology*; D003807:Dentin Sensitivity / Q000532:radiotherapy*; D005898:Glass; D006801:Humans; D053844:Lasers, Solid-State / Q000627:therapeutic use*
chemical_list: D003802:Dentifrices; D057133:Dentin Desensitizing Agents; C510984:NovaMin
keywords: Dentifrices; Dentin; Dentin hypersensitivity; Desensitizers; Laser; Nd:YAG