title: A novel SERS-based magnetic aptasensor for prostate specific antigen assay with high sensitivity.
abstract: The accurate and highly sensitive detection of prostate specific antigen (PSA) is particularly important, especially for obese men and patients. In this report, we present a novel aptamer-based surface-enhanced Raman scattering (SERS) sensor that employs magnetic nanoparticles (MNPs) core-Au nanoparticles (AuNPs) satellite assemblies to detect PSA. The high specific biorecognition between aptamer and PSA caused the dissolution of the core-satellite assemblies, thus the concentration of functionalized AuNPs (signal probes) existing in the supernatant was on the rise with the continual addition of PSA. The aptamer-modified MNPs were used as supporting materials and separation tools in the present sensor. With the assistance of magnet, the mixture was removed from the supernatant for the concentration effects. It was found that the corresponding SERS signals from the supernatant were in direct correlation to PSA concentrations over a wide range and the limit of detection (LOD) was as low as 5.0pg/mL. Excellent recovery was also obtained to assess the feasibility of this method for human serum samples detection. All of these results show a promising application of this method. And this novel sensor can be used for the accurate and highly sensitive detection of PSA in clinic samples in the future.
authors.affiliation: MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Electronic address: yjhu@scnu.edu.cn.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Dong
authors.affiliation: Laboratory of Biosensors & Nanomachines, Département de Chimie, Université de Montréal, Québec, Canada.
authors.forename: Guichi
authors.initials: G
authors.lastname: Zhu
authors.affiliation: MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Tingfeng
authors.initials: T
authors.lastname: Zhu
authors.affiliation: MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Ningjing
authors.initials: N
authors.lastname: Jiang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood* / Q000302:isolation & purification; D011471:Prostatic Neoplasms / Q000097:blood* / Q000473:pathology
chemical_list: D052157:Aptamers, Nucleotide; D058185:Magnetite Nanoparticles; D017430:Prostate-Specific Antigen
keywords: Aptamer; Core-satellite assemblies; Magnetic nanoparticles; PSA; SERS