title: In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
abstract: Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting. Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied. Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content. The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent. The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image. LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins. The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, China. Electronic address: zhen.ma@mail.mcgill.ca.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Blvd West, St. Hyacinthe, Quebec J2S 8E3, Canada.
authors.forename: Joyce I
authors.initials: JI
authors.lastname: Boye
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Hu
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005504:Food Analysis; D005511:Food Handling; D018525:Germination; D006358:Hot Temperature; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D009753:Nutritive Value; D000078724:Pea Proteins / Q000032:analysis*; D018532:Pisum sativum / Q000737:chemistry*; D012525:Saskatchewan; D013213:Starch / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013634:Tannins / Q000032:analysis; D014357:Trypsin / Q000032:analysis
chemical_list: D000596:Amino Acids; D000078724:Pea Proteins; D013634:Tannins; D013213:Starch; D014357:Trypsin
keywords: In vitro digestibility; Pisum sativum; Processing; Protein composition; SDS-polyacrylamide gel electrophoresis; Techno-functional property