title: [Evaluation of hemogram in patients with homozygous sickle cell disease: about 87 cases].
abstract: Homozygous sickle cell disease is one of the most frequent haemoglobinopathies in Morocco. Sickle cell disease is characterized by a large clinical and biological expression variability which depends on modulating genetic and environmental factors. Clinical manifestation includes regenerative anemia whose severity may vary among individuals. In the absence of treatment, it results in premature death. Sickle cell disease is characterized by a large clinical and biological expression variability which depends on genetic and environmental factors. A severe clinical picture marked by high early transfusion frequency, severe infectious complications and early mortality. A constant inflammatory condition characterized by elevated inflammatory proteins and compromised nutritional status. The objective of this study is to determine the hematological parameters profile in moroccan patients with homozygous sickle cell (SS) disease during stationary phases. We conducted a cross-sectional descriptive study of 87 patients with sickle cell (SS) disease. We performed a biological study based on: Hemogram with morphological assessment of red blood cells stained with MGG and automated reticulocyte counting; Hemoglobin electrophoresis test performed on alkaline agarose gel (pH 8.8) and densitometric integration. The average age is 13.22 years ± 16.36, ranging betrween 0.6 and 36 years, with a sex ratio (M/F) of 1.175. Biological effects of anemia were intense in 88.5% of patients; 67.8% of patients had normocytic anemia compared with 29.9% with microcytosis, and 2.3% with macrocytosis. The degree of anisocytosis was related to the degree of anemia, very evocative in patients with homozygous S/S (95.4%). Reticulocytosis was observed in 81.6% of patients; 52.9% of patients had thrombocytosis. Leukocytosis was observed in 64.4% of patients; 80.5% of patients had neutropenia. The parameters of the hemogram will serve as a basis for comparison during crises and will make it possible to evaluate the effectiveness of patient management. High white blood cell count, platelets and MCHC seem to be determinant of sickle cell anemia severity in Morocco. The haematological profile of moroccan patients with sickle cell disease exhibits data similar to those reported in literature relating to patients with leucocytosis from Central Africa. The results of our study suggest that sickle cell anemia is the most common health problem in Morocco and they are similar to those for major sickle cell syndrome.
authors.affiliation: Équipe de Recherche en Hématologie, Laboratoire d'Hématologie, Faculté de Médecine et de Pharmacie, Université Mohammed V, Rabat, Maroc; Laboratoire Central d'Hématologie, Centre Hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Fatima
authors.initials: F
authors.lastname: Dahmani
authors.affiliation: Équipe de Recherche en Hématologie, Laboratoire d'Hématologie, Faculté de Médecine et de Pharmacie, Université Mohammed V, Rabat, Maroc; Laboratoire Central d'Hématologie, Centre Hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Souad
authors.initials: S
authors.lastname: Benkirane
authors.affiliation: Laboratoire Central d'Hématologie, Centre Hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Jaafar
authors.initials: J
authors.lastname: Kouzih
authors.affiliation: Laboratoire Central d'Hématologie, Centre Hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Aziz
authors.initials: A
authors.lastname: Woumki
authors.affiliation: Laboratoire Central d'Hématologie, Centre Hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Hassan
authors.initials: H
authors.lastname: Mamad
authors.affiliation: Équipe de Recherche en Hématologie, Laboratoire d'Hématologie, Faculté de Médecine et de Pharmacie, Université Mohammed V, Rabat, Maroc; Laboratoire Central d'Hématologie, Centre Hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Azlarab
authors.initials: A
authors.lastname: Masrar
mesh_terms: D000293:Adolescent; D000328:Adult; D000755:Anemia, Sickle Cell / Q000503:physiopathology*; D001772:Blood Cell Count / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007223:Infant; D007964:Leukocytosis / Q000453:epidemiology*; D008297:Male; D009018:Morocco; D009503:Neutropenia / Q000453:epidemiology*; D045262:Reticulocytosis / Q000502:physiology; D012720:Severity of Illness Index; D013922:Thrombocytosis / Q000453:epidemiology; D055815:Young Adult
keywords: Morocco; Sickle cell anemia; anemia; hematology