title: Novel adipokines: methodological utility in human obesity research.
abstract: Adipokines could pose a link between adiposity, systemic inflammation and metabolic disease risk. However, it is unclear whether representative biomarkers are methodologically suitable for use in human obesity research.
  
  
  
  We assessed the intra-individual reproducibility of selected adipokines in a sample of 207, apparently healthy, participants with available biosample collections over a 4-month period. Concentrations of the following adipokines were measured at each sampling time point: fatty-acid binding protein-4 (FABP-4), lipocalin-2, monocyte chemoattractant protein 1 (MCP-1), procalcitonin, progranulin, vaspin and visfatin/Nampt. We calculated intraclass correlation coefficients (ICC) and examined Bland-Altman plots.
  
  
  
  The analyses suggested an overall good to excellent biomarker reproducibility over 4 months: FABP-4: ICC=0.73 (95% confidence interval: 0.65, 0.78), lipocalin-2: 0.64 (0.55, 0.71), MCP-1: 0.85 (0.81; 0.89), procalcitonin: 0.78 (0.72, 0.83), progranulin: 0.59 (0.50, 0.68) and vaspin: 0.86 (0.82, 0.89). A good agreement of the repeated measurements was further supported by the Bland-Altman plots. No substantial differences in biomarker performance according to adiposity status could be observed. Reliability of visfatin/Nampt could not be assessed due to a high number of measurements below the lower limit of detection.
  
  
  
  Results suggest that single measurements of the evaluated adipokines could be used in population-based studies aimed to assess links between obesity, inflammation and metabolic diseases.
authors.affiliation: Nutrition, Immunity and Metabolism Start-up Lab, Department of Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.
authors.forename: F
authors.identifier: 0000-0002-3975-5596
authors.initials: F
authors.lastname: Eichelmann
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, DIfE, Nuthetal, Germany.|Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany.|Spital Bülach, Bülach, Switzerland.|German Center of Diabetes Research (DZD), Germany.
authors.forename: N
authors.initials: N
authors.lastname: Rudovich
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, DIfE, Nuthetal, Germany.|Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany.|German Center of Diabetes Research (DZD), Germany.
authors.forename: A F
authors.initials: AF
authors.lastname: Pfeiffer
authors.affiliation: German Center of Diabetes Research (DZD), Germany.|Department of Molecular Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.
authors.forename: M B
authors.initials: MB
authors.lastname: Schulze
authors.affiliation: Institute of Epidemiology, Christian-Albrechts University Kiel, Kiel, Germany.
authors.forename: R D
authors.initials: RD
authors.lastname: Giuseppe
authors.affiliation: Department of Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Boeing
authors.affiliation: Nutrition, Immunity and Metabolism Start-up Lab, Department of Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Aleksandrova
mesh_terms: D054392:Adipokines / Q000378:metabolism*; D050154:Adiposity / Q000502:physiology*; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D035843:Biomedical Research / Q000379:methods* / Q000639:trends; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005858:Germany; D006801:Humans; D007249:Inflammation / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D007333:Insulin Resistance; D008297:Male; D008659:Metabolic Diseases / Q000209:etiology / Q000503:physiopathology*; D008875:Middle Aged; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D009765:Obesity / Q000150:complications / Q000378:metabolism / Q000503:physiopathology*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D015203:Reproducibility of Results; D055105:Waist Circumference
chemical_list: D054392:Adipokines; D015415:Biomarkers; D016207:Cytokines; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human