title: A Biocatalytic Nanomaterial for the Label-Free Detection of Virus-Like Particles.
abstract: The design of nanomaterials that are capable of specific and sensitive biomolecular recognition is an on-going challenge in the chemical and biochemical sciences. A number of sophisticated artificial systems have been designed to specifically recognize a variety of targets. However, methods based on natural biomolecular detection systems using antibodies are often superior. Besides greater affinity and selectivity, antibodies can be easily coupled to enzymatic systems that act as signal amplifiers, thus permitting impressively low detection limits. The possibility to translate this concept to artificial recognition systems remains limited due to design incompatibilities. Here we describe the synthesis of a synthetic nanomaterial capable of specific biomolecular detection by using an internal biocatalytic colorimetric detection and amplification system. The design of this nanomaterial relies on the ability to accurately grow hybrid protein-organosilica layers at the surface of silica nanoparticles. The method allows for label-free detection and quantification of targets at picomolar concentrations.
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: Sabine
authors.initials: S
authors.lastname: Sykora
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: M Rita
authors.initials: MR
authors.lastname: Correro
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: Negar
authors.initials: N
authors.lastname: Moridi
authors.affiliation: Laboratory of Virology, National Reference Center for Enteric Viruses, CHU F. Mitterrand, F and AgroSup Dijon, PAM UMR A 02.102, University of Burgundy-Franche-Comté, 26, Bd Docteur-Petitjean, 21079, Dijon, France.
authors.forename: Gaël
authors.initials: G
authors.lastname: Belliot
authors.affiliation: Laboratory of Virology, National Reference Center for Enteric Viruses, CHU F. Mitterrand, F and AgroSup Dijon, PAM UMR A 02.102, University of Burgundy-Franche-Comté, 26, Bd Docteur-Petitjean, 21079, Dijon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Pothier
authors.affiliation: INOFEA AG, Hochbergerstrasse 60C, 4057, Basel, Switzerland.
authors.forename: Yves
authors.initials: Y
authors.lastname: Dudal
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.|School of the Environment, Nanjing University, Xianlin Ave 63, 210093, Nanjing, China.
authors.forename: Philippe F-X
authors.initials: PF
authors.lastname: Corvini
authors.affiliation: School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
authors.forename: Patrick
authors.identifier: 0000-0002-1743-0387
authors.initials: P
authors.lastname: Shahgaldian
mesh_terms: D055162:Biocatalysis; D054802:Molecular Imprinting / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification*
keywords: biocatalysis; molecular recognition; nanoparticles; organosilica; viruses