title: Microfluidic Arrayed Lab-On-A-Chip for Electrochemical Capacitive Detection of DNA Hybridization Events.
abstract: A microfluidic electrochemical lab-on-a-chip (LOC) device for DNA hybridization detection has been developed. The device comprises a 3 × 3 array of microelectrodes integrated with a dual layer microfluidic valved manipulation system that provides controlled and automated capabilities for high throughput analysis of microliter volume samples. The surface of the microelectrodes is functionalized with single-stranded DNA (ssDNA) probes which enable specific detection of complementary ssDNA targets. These targets are detected by a capacitive technique which measures dielectric variation at the microelectrode-electrolyte interface due to DNA hybridization events. A quantitative analysis of the hybridization events is carried out based on a sensing modeling that includes detailed analysis of energy storage and dissipation components. By calculating these components during hybridization events the device is able to demonstrate specific and dose response sensing characteristics. The developed microfluidic LOC for DNA hybridization detection offers a technology for real-time and label-free assessment of genetic markers outside of laboratory settings, such as at the point-of-care or in-field environmental monitoring.
authors.affiliation: Department of Biomedical Engineering, Ben-Gurion University of the Negev, Beer Sheva, 8410501, Israel. benyoav@bgu.ac.il.
authors.forename: Hadar
authors.initials: H
authors.lastname: Ben-Yoav
authors.affiliation: MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, College Park, MD, 20742, USA.|Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Dykstra
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
authors.affiliation: MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, College Park, MD, 20742, USA.|Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghodssi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D015342:DNA Probes; D004277:DNA, Single-Stranded; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded
keywords: Biosensor; DNA hybridization sensing; Dielectric spectroscopy; Electrical equivalent model; Electrochemical impedance spectroscopy; Faradaic redox reaction; Lab-on-a-chip; Label-free detection; Microfluidics