title: Demonstration of sucrase immunoreactivity of the brush border induced by duodenal mesenchyme in chick stomach endoderm.
abstract: When stomach endoderm of chick embryos was recombined and cultured with duodenal mesenchyme, the endoderm developed a brush border structure over a large area and also differentiated into mucous cells in a small area according to its own developmental fate. In the present investigation, we examined whether the induced brush border structure expressed sucrase antigen by immunoelectron microscopy using the antiserum raised against chicken sucrase. Sucrase immunoreactivity could be detected as ferritin particles in the region where the brush border was induced, whereas it was never detected on microvilli of endodermal cells which differentiated into the mucous cells. Thus, almost all of the endodermal cells could be identified as either small intestine-type cells possessing the sucrase antigen or stomach-type cells possessing mucous granules but not the sucrase antigen. The results indicate that stomach endodermal cells of chick embryos can differentiate not only morphologically but also functionally into typical intestinal epithelial cells under the inductive influence of the duodenal mesenchyme.
authors.affiliation: Department of Anatomy, Dokkyo University School of Medicine, 321-02, Mibu, Tochigi, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ishizuya-Oka
authors.affiliation: Department of Biology, Faculty of Pharmaceutical Sciences, Teikyo University, 199-01, Sagamiko, Kanagawa, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Mizuno
keywords: Immunoelectron microscopy; Mesenchymal induction; Stomach endoderm; Sucrase-Brush border