title: Circulating Concentrations of Adipocytokines and Their Receptors in the Isolated Corpus Cavernosum and Femoral Artery from Trained Rats on a High-Fat Diet.
abstract: The aim of the present study was to evaluate different signaling pathways by which exercise training would interfere in endothelial function in obesity. Therefore, we examined adipocytokine levels and their receptors in the corpus cavernosum and femoral artery from trained rats on a high-fat diet.
  
  
  
  Functional experiments were performed in control sedentary and trained rats, and sedentary (h-SD) and trained male Wistar rats on a high-fat diet (h-TR). Nitric oxide (NO) and reactive oxygen species (ROS) were evaluated in vascular tissue. Circulating adipocytokines and their receptors were analyzed.
  
  
  
  In the h-SD group, the maximal responses to acetylcholine (ACh) were reduced in the femoral artery and corpus cavernosum as well as the electrical field stimulation, accompanied by an increase in circulating insulin, leptin, TNF-α, MCP-1, and PAI-1. Downregulation of ObR protein expression in the femoral artery was observed without alterations in AdipoR1 and TNFR1 in both preparations. A positive effect was observed in the h-TR group regarding the relaxation response to ACh and circulating adipocytokines, resulting in increased NO production and reduced ROS generation. Exercise restored the ObR protein expression only in the femoral artery.
  
  
  
  Aerobic exercise training ameliorated the inflammatory adipocytokines and restored the relaxation responses in the corpus cavernosum and femoral artery in rats on a high-fat diet.
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Amanda C S
authors.initials: AC
authors.lastname: Sponton
authors.forename: Fábio H
authors.initials: FH
authors.lastname: Silva
authors.forename: Hygor N
authors.initials: HN
authors.lastname: Araujo
authors.forename: Carmem P
authors.initials: CP
authors.lastname: Valgas da Silva
authors.forename: Camila
authors.initials: C
authors.lastname: de Moraes
authors.forename: Edson
authors.initials: E
authors.lastname: Antunes
authors.forename: Angelina
authors.initials: A
authors.lastname: Zanesco
authors.forename: Maria A
authors.initials: MA
authors.lastname: Delbin
mesh_terms: D054392:Adipokines / Q000097:blood*; D000818:Animals; D059305:Diet, High-Fat; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005263:Femoral Artery / Q000187:drug effects / Q000378:metabolism*; D018836:Inflammation Mediators / Q000097:blood; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010413:Penis / Q000098:blood supply*; D010805:Physical Conditioning, Animal; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D054417:Receptors, Adipokine / Q000378:metabolism*; D054419:Receptors, Adiponectin / Q000378:metabolism; D054411:Receptors, Leptin / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism; D057185:Sedentary Behavior; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D054392:Adipokines; D018836:Inflammation Mediators; D017382:Reactive Oxygen Species; D054417:Receptors, Adipokine; D054419:Receptors, Adiponectin; D054411:Receptors, Leptin; D047888:Receptors, Tumor Necrosis Factor, Type I; C506237:Tnfrsf1a protein, rat; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; C504339:adiponectin receptor 1, rat; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D013482:Superoxide Dismutase