title: Simulated herbivory in chickpea causes rapid changes in defense pathways and hormonal transcription networks of JA/ethylene/GA/auxin within minutes of wounding.
abstract: Chickpea (C. arietinum L.) is an important pulse crop in Asian and African countries that suffers significant yield losses due to attacks by insects like H. armigera. To obtain insights into early responses of chickpea to insect attack, a transcriptomic analysis of chickpea leaves just 20 minutes after simulated herbivory was performed, using oral secretions of H. armigera coupled with mechanical wounding. Expression profiles revealed differential regulation of 8.4% of the total leaf transcriptome with 1334 genes up-regulated and 501 down-regulated upon wounding at log2-fold change (|FC| ≤ -1 and ≥1) and FDR value ≤ 0.05. In silico analysis showed the activation of defenses through up-regulation of genes of the phenylpropanoid pathway, pathogenesis, oxidases and CYTP450 besides differential regulation of kinases, phosphatases and transcription factors of the WRKY, MYB, ERFs, bZIP families. A substantial change in the regulation of hormonal networks was observed with up-regulation of JA and ethylene pathways and suppression of growth associated hormone pathways like GA and auxin within 20 minutes of wounding. Secondary qPCR comparison of selected genes showed that oral secretions often increased differential expression relative to mechanical damage alone. The studies provide new insights into early wound responses in chickpea.
authors.affiliation: Plant Gene Expression Lab, CSIR-National Botanical Research Institute, Lucknow-226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, Rafi Marg, New Delhi-110 001, India.
authors.forename: Saurabh Prakash
authors.initials: SP
authors.lastname: Pandey
authors.affiliation: Plant Gene Expression Lab, CSIR-National Botanical Research Institute, Lucknow-226001, India.
authors.forename: Shruti
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, Rafi Marg, New Delhi-110 001, India.|Dept of Bioinformatics CSIR-National Botanical Research Institute, Lucknow-226001, India.
authors.forename: Ridhi
authors.initials: R
authors.lastname: Goel
authors.affiliation: Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, Rafi Marg, New Delhi-110 001, India.|Dept of Bioinformatics CSIR-National Botanical Research Institute, Lucknow-226001, India.
authors.forename: Deepika
authors.initials: D
authors.lastname: Lakhwani
authors.affiliation: Plant Gene Expression Lab, CSIR-National Botanical Research Institute, Lucknow-226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, Rafi Marg, New Delhi-110 001, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Singh
authors.affiliation: Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, Rafi Marg, New Delhi-110 001, India.|Dept of Bioinformatics CSIR-National Botanical Research Institute, Lucknow-226001, India.
authors.forename: Mehar Hasan
authors.initials: MH
authors.lastname: Asif
authors.affiliation: Plant Gene Expression Lab, CSIR-National Botanical Research Institute, Lucknow-226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, Rafi Marg, New Delhi-110 001, India.
authors.forename: Aniruddha P
authors.initials: AP
authors.lastname: Sane
mesh_terms: D000818:Animals; D029863:Cicer / Q000235:genetics* / Q000276:immunology; D003517:Cyclopentanes / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D005030:Ethylenes / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics*; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism*; D060434:Herbivory / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D009036:Moths / Q000502:physiology; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012463:Saliva / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D005875:Gibberellins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; C011006:jasmonic acid; C036216:ethylene; C007842:gibberellic acid