title: Corymbulosins D-H, 2-Hydroxy- and 2-Oxo-clerodane Diterpenes from the Bark of Laetia corymbulosa.
abstract: A bioactive CH3OH-CH2Cl2(1:1) extract of the bark of Laetia corymbulosa provided five new clerodane diterpenes with an isozuelanin skeleton, designated as corymbulosins D-H (1-5), as well as the known corymbulosins B (6) and C (7), for which the relative configurations were not previously determined. The structures of 1-5 were characterized on the basis of 1D and 2D NMR spectroscopic and HRMS analysis. The absolute configurations of all isolated compounds 1-7 were verified through chemical methods, including modified Mosher esterifications or oxidation of the hydroxy group at C-2, ECD experiments, and spectroscopic data comparison. The isolated compounds were evaluated for antiproliferative activity against a small panel of human cancer cell lines.
authors.affiliation: School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192, Japan.
authors.forename: Airi
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Saito
authors.affiliation: School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Fukuyoshi
authors.affiliation: Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-7568, United States.
authors.forename: Masuo
authors.initials: M
authors.lastname: Goto
authors.affiliation: Tokyo University of Pharmacy and Life Sciences , 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Miyake
authors.affiliation: NIH Special Volunteer , Wayne, Pennsylvania 19087, United States.
authors.forename: David J
authors.initials: DJ
authors.lastname: Newman
authors.forename: Barry R
authors.initials: BR
authors.lastname: O'Keefe
authors.affiliation: Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-7568, United States.|Chinese Medicine Research and Development Center, China Medical University and Hospital , 2 Yuh-Der Road, Taichung, 40447, Taiwan.
authors.forename: Kuo-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.affiliation: School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University , Kanazawa 920-1192, Japan.|Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-7568, United States.
authors.forename: Kyoko
authors.identifier: 0000-0002-1642-6538
authors.initials: K
authors.lastname: Nakagawa-Goto
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010568:Peru; D024301:Plant Bark / Q000737:chemistry*; D031308:Salicaceae / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane