title: EMA, an epithelial membrane-associated antigen during early development and morphogenesis ofXenopus laevis.
abstract: We raised monoclonal antibodies against a membrane fraction ofXenopus neurulae in order to detect tissue-specific cell-surface markers. Here we describe a monoclonal antibody that recognizes an epithelial membrane-associated antigen (EMA) in immunohistological stainings. The tissue-specific and membrane-associated antigen detected in immunohistological stainings could serve as useful marker in epithelium differentiation and membrane organization of the early embryo. In tadpoles and adults EMA was found in specific epithelial tissues derived from different germ layers such as kidney, skin, gut, pancreas, epiphysis and choroid plexus. In the cleaving embryo this antibody stained newly formed membranes between blastomeres from the two-cell stage onwards. Cytoplasmic staining in large oocytes and early embryos was also observed. The possibility that the cytoplasmic signal represents a maternal store of membrane material is discussed.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freien Universität, Arnimallee 22, 1000, Berlin 33, Germany.
authors.forename: Bärbel
authors.initials: B
authors.lastname: Kiene
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freien Universität, Arnimallee 22, 1000, Berlin 33, Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Wedlich
keywords: Epithelium marker; Monoclonal antibody; Plasma membrane; Xenopus development