title: iTRAQ-MS/MS Proteomic Analysis Reveals Differentially Expressed Proteins During Post-harvest Maturation of the White Button Mushroom Agaricus bisporus.
abstract: Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify protein expression changes that occur during the post-harvest maturation of Agaricus bisporus fruitbodies at 0, 6, 12, and 48 h after harvest. A total of 5878 unique peptides, representing 1063 proteins, were identified. Quantitative data were obtained from 1012 out of the 1063 proteins. A total of 102, 106, and 160 differentially expressed proteins (>twofolds differences) were identified from samples collected at 6, 12, and 48 h compared to sample from 0 h post harvest, accounting for 10.1, 10.5, and 15.8% of the total proteins identified, respectively. All identified proteins including the differentially expressed proteins among different post-harvest stages were subjected to bioinformatic analysis. Furthermore, seven representative proteins either up or down-regulated at different post-harvest stages were analyzed by real-time PCR. Three out of the seven proteins, the mismatched base pair and cruciform DNA recognition protein, hydrophobin-B and protein transporter, exhibited similar expression patterns as their corresponding proteins. The 260 differentially expressed proteins identified from our study laid a foundation for future studies aiming to understand the post-harvest maturation process of A. bisporus and eventually, might help in the development of breeding program to identify strains with extended shelf life.
authors.affiliation: The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, No.10, 95 Lane, Qianheng Rd., Fuzhou, 350014, People's Republic of China.
authors.forename: Meiyuan
authors.initials: M
authors.lastname: Chen
authors.affiliation: The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, No.10, 95 Lane, Qianheng Rd., Fuzhou, 350014, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liao
authors.affiliation: The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, No.10, 95 Lane, Qianheng Rd., Fuzhou, 350014, People's Republic of China.
authors.forename: Hongrong
authors.initials: H
authors.lastname: Li
authors.affiliation: The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, No.10, 95 Lane, Qianheng Rd., Fuzhou, 350014, People's Republic of China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Cai
authors.affiliation: The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, No.10, 95 Lane, Qianheng Rd., Fuzhou, 350014, People's Republic of China.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Sylvan Research, West Hills Industrial Park, Bldg 2, Kittanning, PA, 16201, USA.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Wach
authors.affiliation: The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, No.10, 95 Lane, Qianheng Rd., Fuzhou, 350014, People's Republic of China. zeshengwang1026@126.com.
authors.forename: Zesheng
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000364:Agaricus / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D048690:Fruiting Bodies, Fungal; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D010641:Phenotype; D020543:Proteome; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D005656:Fungal Proteins; D020543:Proteome