title: Briarenols C-E, New Polyoxygenated Briaranes from the Octocoral Briareum excavatum.
abstract: Three new polyoxygenated briarane diterpenoids, briarenols C-E (1-3), were isolated from the octocoralBriareum excavatum. The structures of briaranes1-3were elucidated by interpretation of spectroscopic data, and the methylenecyclohexane ring in1was found to exist in a twisted boat conformation. Briarenol D (2) displayed an inhibitory effect on the release of elastase by human neutrophils with an IC50value of 4.65 μM. Briarenol E (3) was found to inhibit the protein expression of pro-inflammatory inducible nitric oxide synthase (iNOS) in a murine macrophage-like cell line, RAW 264.7, stimulated with lipopolysaccharides (LPS).
authors.affiliation: Division of Neurosurgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 802, Taiwan. chen06688@gmail.com.|Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan. chen06688@gmail.com.
authors.forename: Nan-Fu
authors.initials: NF
authors.lastname: Chen
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. gobetter04@gmail.tw.
authors.forename: Yin-Di
authors.initials: YD
authors.lastname: Su
authors.affiliation: Graduate Institute of Natural Products, College of Medicine and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.|Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.|Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. liao771107@gmail.com.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 804, Taiwan. liao771107@gmail.com.
authors.forename: Zuo-Jian
authors.initials: ZJ
authors.lastname: Liao
authors.affiliation: Department of Obstetrics and Gynecology, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan. khtsui60@gmail.com.|Department of Obstetrics and Gynecology and Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan. khtsui60@gmail.com.|Department of Biological Science, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. khtsui60@gmail.com.|Department of Pharmacy and Master Program, College of Pharmacy and Health Care, Tajen University, Pingtung 907, Taiwan. khtsui60@gmail.com.
authors.forename: Kuan-Hao
authors.initials: KH
authors.lastname: Tsui
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 804, Taiwan. wzh@mail.nsysu.edu.tw.|Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. wzh@mail.nsysu.edu.tw.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.affiliation: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. yachwu@kmu.edu.tw.|Research Center for Natural Products and Drug Development, Kaohsiung Medical University, Kaohsiung 807, Taiwan. yachwu@kmu.edu.tw.|Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. yachwu@kmu.edu.tw.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.|Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. pjsung@nmmba.gov.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. pjsung@nmmba.gov.tw.|Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan. pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry*; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D004224:Diterpenes
keywords: Briareum excavatum; anti-inflammatory; briarane; briarenol; elastase; iNOS; octocoral