title: Novel immunosuppressive pregnane glycosides from the leaves of Epigynum auritum.
abstract: Phytochemical investigation on the leaves of Epigynum auritum led to the isolation of three novel C21pregnane glycosides, epigynosides, E-G (1-3), together with two known pregnane glycosides, epigynosides A (4) and C (5). Their structures were elucidated based on extensive spectroscopic data (MS, IR, 1D and 2D NMR) analysis, as well as comparison with the reported data. The immunological activities of the new compounds was evaluated against concanavalin A (Con A)-stimulated proliferation of mice splenocyte in vitro. Compounds 1-3 displayed significant immunosuppressive activities, close to the efficacy of the positive control (dexamethasone) at the concentration of 50μM.
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China.
authors.forename: Yuan-Cheng
authors.initials: YC
authors.lastname: Yao
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China.
authors.forename: Sheng-Bao
authors.initials: SB
authors.lastname: Cai
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China.
authors.forename: Tian-Rui
authors.initials: TR
authors.lastname: Zhao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors.forename: Xiao-Nian
authors.initials: XN
authors.lastname: Li
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China. Electronic address: jxcao@kmust.edu.cn.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Cao
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 6505000, People's Republic of China. Electronic address: ggcheng@kmust.edu.cn.
authors.forename: Gui-Guang
authors.initials: GG
authors.lastname: Cheng
mesh_terms: D000818:Animals; D029062:Apocynaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D011278:Pregnanes / Q000737:chemistry* / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
chemical_list: D006027:Glycosides; D007166:Immunosuppressive Agents; D011278:Pregnanes; C574437:pregnane glycoside
keywords: Epigynosides E–G; Epigynum auritum; Immunosuppressive; Pregnane glycosides