title: TP53 and ATM mRNA expression in skin and skeletal muscle after low-level laser exposure.
abstract: Low-level lasers are widespread in regenerative medicine, but the molecular mechanisms involved in their biological effects are not fully understood, particularly those on DNA stability. Therefore, this study aimed to investigate mRNA expression of genes related to DNA genomic stability in skin and skeletal muscle tissue from Wistar rats exposed to low-level red and infrared lasers. For this, TP53 (Tumor Protein 53) and ATM (Ataxia Telangiectasia Mutated gene) mRNA expressions were evaluated by real-time quantitative PCR (RT-qPCR) technique 24 hours after low-level red and infrared laser exposure. Our data showed that relative TP53 mRNA expression was not significantly altered in both tissues exposed to lasers. For ATM, relative mRNA expression in skin tissue was not significantly altered, but in muscle tissue, laser exposure increased relative ATM mRNA expression. Low-level red and infrared laser radiations alter ATM mRNA expression related to DNA stability in skeletal muscle tissue.
authors.affiliation: a Departamento de Ciências Fisiológicas , Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Guedes de Almeida
authors.affiliation: b Departamento de Biofísica e Biometria , Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Luiz Philippe da Silva
authors.initials: LPDS
authors.lastname: Sergio
authors.affiliation: c Departamento de Morfologia , Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora , Juiz de Fora , Brazil.
authors.forename: Flavia
authors.initials: F
authors.lastname: de Paoli
authors.affiliation: b Departamento de Biofísica e Biometria , Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Andre Luiz
authors.initials: AL
authors.lastname: Mencalha
authors.affiliation: a Departamento de Ciências Fisiológicas , Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro , Rio de Janeiro , Brazil.|b Departamento de Biofísica e Biometria , Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro , Rio de Janeiro , Brazil.|d Centro de Ciências da Saúde , Centro Universitário Serra dos Órgãos , Rio de Janeiro , Brazil.
authors.forename: Adenilson de Souza
authors.initials: AS
authors.lastname: da Fonseca
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins / Q000378:metabolism*; D015870:Gene Expression; D028022:Low-Level Light Therapy; D018482:Muscle, Skeletal / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D064007:Ataxia Telangiectasia Mutated Proteins
keywords: DNA; RT-qPCR; Wistar rats; gene expression; laser