title: BRASSINOSTEROID INSENSITIVE2 negatively regulates cellulose synthesis inArabidopsisby phosphorylating cellulose synthase 1.
abstract: The deposition of cellulose is a defining aspect of plant growth and development, but regulation of this process is poorly understood. Here, we demonstrate that the protein kinase BRASSINOSTEROID INSENSITIVE2 (BIN2), a key negative regulator of brassinosteroid (BR) signaling, can phosphorylateArabidopsiscellulose synthase A1 (CESA1), a subunit of the primary cell wall cellulose synthase complex, and thereby negatively regulate cellulose biosynthesis. Accordingly, point mutations of the BIN2-mediated CESA1 phosphorylation site abolished BIN2-dependent regulation of cellulose synthase activity. Hence, we have uncovered a mechanism for how BR signaling can modulate cellulose synthesis in plants.
authors.affiliation: Department of Biology, Eidgenössiche Technische Hochschule Zurich, 8092 Zurich, Switzerland.
authors.forename: Clara
authors.initials: C
authors.lastname: Sánchez-Rodríguez
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557.
authors.forename: KassaDee
authors.initials: K
authors.lastname: Ketelaar
authors.affiliation: School of Biosciences, University of Melbourne, Parkville 3010, VIC, Australia.
authors.forename: Rene
authors.initials: R
authors.lastname: Schneider
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Villalobos
authors.affiliation: Energy Biosciences Institute, University of California, Berkeley, CA 94720 crs@berkeley.edu staffan.persson@unimelb.edu.au iwallace@unr.edu.
authors.forename: Chris R
authors.initials: CR
authors.lastname: Somerville
authors.affiliation: School of Biosciences, University of Melbourne, Parkville 3010, VIC, Australia; crs@berkeley.edu staffan.persson@unimelb.edu.au iwallace@unr.edu.
authors.forename: Staffan
authors.initials: S
authors.lastname: Persson
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557; crs@berkeley.edu staffan.persson@unimelb.edu.au iwallace@unr.edu.
authors.forename: Ian S
authors.initials: IS
authors.lastname: Wallace
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017354:Point Mutation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D029681:Arabidopsis Proteins; D002482:Cellulose; D005964:Glucosyltransferases; D011494:Protein Kinases; C110227:CESA1 protein, Arabidopsis; C478648:cellulose synthase; C448985:At4g18710 protein, Arabidopsis
keywords: brassinosteroid; cellulose synthase; plant cell wall; protein kinase; regulation