title: Isolation, molecular characterization and screening of indigenous lactobacilli for their abilities to produce bioactive conjugated linoleic acid (CLA).
abstract: Ingestion of conjugated linoleic acid poised many health benefits; however, amount of CLA one can get through generalized diet in is inadequate in exerting the desired benefits. Therefore, presence of CLA producing lactobacilli in dairy fermented foods has a tremendous potential to increase the CLA content. Therefore, present study was focused to isolate and characterize CLA producing lactobacilli from different dairy products and human faeces. Arguably, 283 lactobacilli were isolated from various sources and tested for CLA production. Fifty-seven CLA producing (≥20 µg/ml) lactobacilli were selected from screening in de Man, Rogosa and Sharpe (MRS) broth and reconstituted with skim milk (SM), supplemented with 0.5 mg/ml of linoleic acid. Positive strains were classified into-L. plantarum(44%),L. gasseri(30%),L. fermentum(21%) andL. salivarius(5%) species. Nineteen most efficient strains (CLA ≥25 µg/ml) were further assessed in SM for CLA production. Total 08 strains produced significantly higher CLA in SM than MRS and also producedcis9,trans11,trans10,cis12 andtrans9,trans11 isomers. Overall,L. plantarumHIF15 was reported as the best producer of CLA and other 08 lactobacilli may be utilized for the formulation of CLA-enriched functional foods to support these bacteria to synthesize CLA in the human gut.
authors.affiliation: Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana 132001 India.
authors.forename: Dinesh Kumar
authors.initials: DK
authors.lastname: Dahiya
authors.affiliation: Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana 132001 India.|College of Dairy Science and Technology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Puniya
keywords: Biohydrogenation; L. plantarum; Linoleic acid; c12; c9; t10; t11; t9