title: Enzymatic site-selectivity enabled by structure-guided directed evolution.
abstract: Biocatalytic site-selective (regioselective) organic transformations have been practiced for decades, but the traditional limitations of enzymes regarding narrow substrate acceptance and the often observed insufficient degree of selectivity have persisted until recently. With the advent of directed evolution, it is possible to engineer site-selectivity to suit the needs of organic chemists. This review features recent progress in this exciting research area, selected examples involving P450 monooxygenases, halogenases and Baeyer-Villiger monooxygenases being featured for illustrative purposes. The complementary nature of enzymes and man-made catalysts is emphasized.
authors.affiliation: Department of Chemistry, Philipps-University Marburg, Hans-Meerwein Strasse 4, 35032, Marburg, Germany and Max-Plank-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim, Germany. reetz@mpi-muelheim.mpg.de.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Philipps-University Marburg, Hans-Meerwein Strasse 4, 35032, Marburg, Germany and Max-Plank-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim, Germany. reetz@mpi-muelheim.mpg.de.
authors.forename: Guangyue
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Chemistry, Philipps-University Marburg, Hans-Meerwein Strasse 4, 35032, Marburg, Germany and Max-Plank-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim, Germany. reetz@mpi-muelheim.mpg.de.
authors.forename: Manfred T
authors.identifier: http://orcid.org/0000-0002-3451-2421
authors.initials: MT
authors.lastname: Reetz
mesh_terms: D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D013237:Stereoisomerism
chemical_list: D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases