title: A Mini-Electrochemical System with Integrated Micropipet Tip and Pencil Graphite Electrode for Measuring Cytotoxicity.
abstract: A novel mini-electrochemical system has been developed for evaluating cytotoxicity of anticancer drugs based on trace cell samples. The mini-electrochemical system was integrated by using pencil graphite modified with threonine as working electrode, an Ag/AgCl reference electrode and a micropipet tip as electrochemical cell. The mini-electrochemical system dramatically reduces sample volumes from 500 μL in a traditional electrochemical system to 10 μL, and exhibits excellent electrocatalytic activity toward oxidation of purine from MCF-7 cells due to increased sensitivity provided by threonine. Moreover, the relationship between peak current and the cell concentration in the range from 3.0 × l03to 7.0 × l06cells/mL was studied, and a nonlinear exponential relationship between them was established over a wide concentration range. In evaluating the effect of anticancer drugs on cell viability, the results of drug cytotoxicity test based on cyclophosphamide were in close agreement with classical 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays. The proposed device is so simple, cheap, and easy to operate that it could be applied to single-use applications. The mini-electrochemical system proved to be a useful tool and can be applied to electrochemical studies of cancer cells as well as other biological samples such as proteins and DNA.
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China. dmwu@jmsu.edu.cn.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wu
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Guo
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Jin-Lian
authors.initials: JL
authors.lastname: Li
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Cui
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Zhou
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154000, China.
authors.forename: Su-E
authors.initials: SE
authors.lastname: Hao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006108:Graphite; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells
chemical_list: D000970:Antineoplastic Agents; D006108:Graphite
keywords: Anticancer drug sensitivity; MCF-7 cells; Micropipet tip; Mini-electrochemical system; Pencil graphite electrode