title: Global metabolome changes induced by cyanobacterial blooms in three representative fish species.
abstract: Cyanobacterial blooms induce important ecological constraints for aquatic organisms and strongly impact the functioning of aquatic ecosystems. In the past decades, the effects of the cyanobacterial secondary metabolites, so called cyanotoxins, have been extensively studied in fish. However, many of these studies have used targeted approaches on specific molecules, which are thought to react to the presence of these specific cyanobacterial compounds. Since a few years, untargeted metabolomic approaches provide a unique opportunity to evaluate the global response of hundreds of metabolites at a glance. In this way, our study provides the first utilization of metabolomic analyses in order to identify the response of fish exposed to bloom-forming cyanobacteria. Three relevant fish species of peri-urban lakes of the European temperate regions were exposed for 96h either to a microcystin (MC)-producing or to a non-MC-producing strain of Microcystis aeruginosa and metabolome changes were characterized in the liver of fish. The results suggest that a short-term exposure to those cyanobacterial biomasses induces metabolome changes without any response specificity linked to the fish species considered. Candidate metabolites are involved in energy metabolism and antioxidative response, which could potentially traduce a stress response of fish submitted to cyanobacteria. These results are in agreement with the already known information and could additionally bring new insights about the molecular interactions between cyanobacteria and fish.
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.. Electronic address: bsotton@mnhn.fr.
authors.forename: Benoît
authors.initials: B
authors.lastname: Sotton
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Paris
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Le Manach
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Blond
authors.affiliation: UMR iEES Paris (CNRS, UPMC, INRA, IRD, AgroParisTech, UPEC), Institute of Ecology and Environmental Sciences - Paris, Université Pierre et Marie Curie, Paris, France; UMS 3194 - CEREEP Ecotron IDF (CNRS, ENS), Ecole Normale Supérieure, Saint-Pierre-Lès-Nemours, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Lacroix
authors.affiliation: UMS 3194 - CEREEP Ecotron IDF (CNRS, ENS), Ecole Normale Supérieure, Saint-Pierre-Lès-Nemours, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Millot
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Duval
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.
authors.forename: Qin
authors.initials: Q
authors.lastname: Qiao
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Catherine
authors.affiliation: UMR 7245 MNHN/CNRS Molécules de communication et adaptation des microorganismes, équipe Cyanobactéries, Cyanotoxines et Environnement, Muséum National d'Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France.. Electronic address: bmarie@mnhn.fr.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Marie
mesh_terms: D000818:Animals; D017753:Ecosystem; D005060:Europe; D005068:Eutrophication; D005399:Fishes / Q000378:metabolism / Q000382:microbiology*; D060106:Lakes; D008099:Liver / Q000382:microbiology; D055442:Metabolome; D052998:Microcystins / Q000494:pharmacology*; D046931:Microcystis
chemical_list: D052998:Microcystins
keywords: Cyanobacteria; Ecotoxicology; Fish; Metabolomics; Microcystin