title: Developmental regulation of presence of binding sites for neoglycoproteins and endogenous lectins in various embryonic stages of human lung, liver and heart.
abstract: Protein-carbohydrate interactions are supposed to play key roles in the mechanisms of cell adhesion, biosignalling and intracellular routing, warranting the analysis of the developmental course of expression of epitopes of this system. Thus, a panel of carrier-immobilized carbohydrate ligands was used as probes, namely lactose,N-acetylgalactosamine,N-acetylglucosamine, mannose, fucose and maltose. Additionally, an antibody to an endogenous β-galactoside-binding lectin (anti-galectin-1), the biotinylated lectin and two further human lectins, namely the macrophage migration inhibitory factor-binding sarcolectin and serum amyloid P component (SAP) that displays selectivity for sulphated sugars and mannose-6-phosphate, were included. They enabled us to assess the extent of the presence of respective binding sites in fixed sections from human lungs (pulmonary epithelial cells), livers (hepatocytes) and hearts (myocard cells) of 10-50 weeks gestation. Invariably, specific binding was detected in the three organ types, at least in certain stages. In most of the cases, the intensity of staining exhibited developmental regulation. The apparent patterns reveal similarities between the different cell types, as seen with immobilizedN-acetylglucosamine as well as with labelled galectin-1 and sarcolectin. However, drastic differences among such patterns with nearly opposite developmental courses do also occur, as detected for carrier-attached mannose and maltose residues. These results point to a potential importance for the detected glycohistochemical features in human development and substantiate the possibility of differential regulation of the presence of binding sites for distinct sugars within a certain organ and between the individual cell types of the monitored organs.
authors.affiliation: Abteilung Pathologie, Thoraxklinik, Amalienstrasse 5, D-69126, Heidelberg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Kayser
authors.affiliation: Institut für Physiologische Chemie, Ticrärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, D-80539, Munchen, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.affiliation: Institut für klinische Pathologie der Universität, Währinger Gürtel 18-20, A-1090, Wien, Austria.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Böhm
authors.affiliation: Institut für Physiologische Chemie, Ticrärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, D-80539, Munchen, Germany.
authors.forename: Sonia
authors.initials: S
authors.lastname: Donaldo-Jacinto
authors.affiliation: Institut fur Pathologie, Robert-Bosch-Krankenhaus, Auerbachstrasse 110, D-70376, Stuttgart, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Fritz
authors.affiliation: Institut für Physiologische Chemie, Ticrärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, D-80539, Munchen, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Kaltner
authors.affiliation: Abteilung Pathologie, Thoraxklinik, Amalienstrasse 5, D-69126, Heidelberg, Germany.
authors.forename: Gian
authors.initials: G
authors.lastname: Kayser
authors.affiliation: Institut fur Pathologie, Postfach 1553, D-58655, Hemer, Germany.
authors.forename: Wolf-Peter
authors.initials: WP
authors.lastname: Kunze
authors.affiliation: Pathologisches Institut der Universität, Thalkirchner Strasse 36, D-80337, Munchen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Nehrlich
authors.affiliation: Institut für Physiologische Chemie, Ticrärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, D-80539, Munchen, Germany.
authors.forename: Fu-Yue
authors.initials: FY
authors.lastname: Zeng
authors.affiliation: Abteilung Pathologie, Thoraxklinik, Amalienstrasse 5, D-69126, Heidelberg, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
keywords: Development; Histochemistry; Lectin; Neoglycoprotein Glycoconjugate