title: Using Small Angle X-Ray Scattering (SAXS) to Characterize the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs).
abstract: Small angle X-ray scattering (SAXS) provides information about the conformation and flexibility of proteins in solution, and hence provides complementary structural information to that obtained from X-ray crystallography and nuclear magnetic resonance spectroscopy. In this chapter, we describe the methods for the preparation of matrix metalloproteinase (MMP) samples for SAXS analyses, and for the acquisition, processing and interpretation of the SAXS data.
authors.affiliation: Institute of Biomedical and Biomolecular Science (IBBS) and School of Biological Sciences, University of Portsmouth, King Henry Building, King Henry I Street, Portsmouth, PO1 2DY, UK.
authors.forename: Louise E
authors.initials: LE
authors.lastname: Butt
authors.affiliation: Institute of Biomedical and Biomolecular Science (IBBS) and School of Biological Sciences, University of Portsmouth, King Henry Building, King Henry I Street, Portsmouth, PO1 2DY, UK.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Holland
authors.affiliation: Institute of Biomedical and Biomolecular Science (IBBS) and School of Biological Sciences, University of Portsmouth, King Henry Building, King Henry I Street, Portsmouth, PO1 2DY, UK.|Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, UK.
authors.forename: Nikul S
authors.initials: NS
authors.lastname: Khunti
authors.affiliation: Institute of Biomedical and Biomolecular Science (IBBS) and School of Biological Sciences, University of Portsmouth, King Henry Building, King Henry I Street, Portsmouth, PO1 2DY, UK.
authors.forename: Debra L
authors.initials: DL
authors.lastname: Quinn
authors.affiliation: Institute of Biomedical and Biomolecular Science (IBBS) and School of Biological Sciences, University of Portsmouth, King Henry Building, King Henry I Street, Portsmouth, PO1 2DY, UK. andy.pickford@port.ac.uk.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Pickford
mesh_terms: D020782:Matrix Metalloproteinases / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D053838:Scattering, Small Angle; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D020782:Matrix Metalloproteinases
keywords: Multi-domain; Oligomer; Protein dynamics; Protein flexibility; Structure–function relationship