title: Desulfurization with Thialkalivibrio versutus immobilized on magnetic nanoparticles modified with 3-aminopropyltriethoxysilane.
abstract: OBJECTIVE
  Thialkalivibrio versutus D301 cells were immobilized on Fe3O4nanoparticles (NPs) synthesized by an improved chemical coprecipitation method and modified with 3-aminopropyltriethoxysilane (APTES), then the immobilized cells were used in sulfur oxidation.
  
  
  RESULTS
  The prepared Fe3O4-APTES NPs had a narrow size distribution (10 ± 2 nm) and were superparamagnetic, with a saturation magnetization of 60.69 emu/g. Immobilized cells had a saturation magnetization of 34.95 emu/g and retained superparamagnetism. The optimum conditions for cell immobilization were obtained at pH 9.5 and 1 M Na+. The immobilization capacity of Fe3O4-APTES NPs was 7.15 g DCW/g-NPs that was 2.3-fold higher than that of Fe3O4NPs. The desulfurization efficiency of the immobilized cells was close to 100%, having the same sulfur oxidation capacity as free cells. Further, the immobilized cells could be reused at least eight times, retaining more than 85% of their desulfurization efficiency.
  
  
  CONCLUSIONS
  Immobilization of cells with the modified magnetic NPs efficiently increased cell controllability, have no effect on their desulfurization activity and could be effectively used in large-scale industrial applications.
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Tingzhen
authors.initials: T
authors.lastname: Mu
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Yueping
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Maohua
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China. jmxing@ipe.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, China. jmxing@ipe.ac.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D018914:Cells, Immobilized / Q000378:metabolism*; D042001:Ectothiorhodospiraceae / Q000378:metabolism*; D017743:Equipment Reuse; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000382:microbiology*; D010084:Oxidation-Reduction; D010316:Particle Size; D011437:Propylamines / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry / Q000378:metabolism*
chemical_list: D058185:Magnetite Nanoparticles; D011437:Propylamines; D012821:Silanes; D013455:Sulfur; C477625:amino-propyl-triethoxysilane
keywords: 3-Aminopropyltriethoxysilane; Cell immobilization; Desulfurization; Fe3O4 nanoparticles; Thialkalivibrio versutus D301