title: Elevated microRNA-520g in pre-eclampsia inhibits migration and invasion of trophoblasts.
abstract: Pre-eclampsia (PE) is a common and severe obstetric complication. MicroRNAs (miRs) have emerged as molecules that are associated with the disease.
  
  
  
  Quantitative reverse transcription PCR (RT-qPCR) was used for serum miR-520g characterization from 19 severe pre-eclamptic and 19 normal pregnancies. In situ hybridation was adopted to localize microRNA-520g (miR-520g). Migration and invasion of HTR-8/SVneo cells were evaluated after miR-520g mimic treatment with transwell system. MiR-520g target gene was verified in luciferase reporter system.
  
  
  
  The expression of serum miR-520g displayed an upward trend as pregnancies progress. At first-trimester, miR-520g in pre-eclampsia was significantly higher than that in the control, but no significant differences were found in the second and last trimesters. MiR-520g localized in cytoplasm of early trimester placental trophoblasts. The migration and invasion of HTR8/SVneo were inhibited by miR-520g mimic treatment. Matrix metalloproteinase 2 (MMP2) was verified as a direct target of miR-520g.
  
  
  
  Elevated maternal serum level of miR-520g level in first trimester was detected in patients with severe PE. By suppressing the migration and invasion of trophoblast via at least partial inhibition of MMP2 translation inhibition, miR-520g might play a role in the defective spiral artery remodeling, and thus contribute to pre-eclampsia pathophysiology.
authors.affiliation: Department of Clinical Laboratory, Shanghai First People's Hospital Baoshan Branch, Shanghai, PR China.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, Shanghai First People's Hospital Baoshan Branch, Shanghai, PR China.
authors.forename: Anxiong
authors.initials: A
authors.lastname: Long
authors.affiliation: Department of Clinical Laboratory, Shanghai First People's Hospital Baoshan Branch, Shanghai, PR China.
authors.forename: Longyi
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Clinical Laboratory, Shanghai First People's Hospital Baoshan Branch, Shanghai, PR China.
authors.forename: Mao
authors.initials: M
authors.lastname: Hong
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People's Hospital Baoshan Branch, Shanghai, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Shanghai First People's Hospital Baoshan Branch, Shanghai, PR China.
authors.forename: Leiming
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Clinical Laboratory, Shanghai First People's Hospital Baoshan Branch, Shanghai, PR China. Electronic address: aqianmerry@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
mesh_terms: D000328:Adult; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D035683:MicroRNAs / Q000097:blood*; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: C575048:MIRN520 microRNA, human; D035683:MicroRNAs; D020778:Matrix Metalloproteinase 2
keywords: Biomarker; HTR8/SVneo; Matrix metalloproteinase 2; Pre-eclampsia; Trophoblast; microRNA-520g