title: Temperature-Controlled Three-Stage Switching of Wetting, Morphology, and Protein Adsorption.
abstract: The novel polymeric coatings of oligoperoxide-graft-poly(4-vinylpyridine-co-oligo(ethylene glycol)ethyl ether methacrylate246) [oligoperoxide-graft-P(4VP-co-OEGMA246)] attached to glass were successfully fabricated. The composition, thickness, morphology, and wettability of resulting coatings were analyzed using X-ray photoelectron spectroscopy, ellipsometry, atomic force microscopy, and contact angle measurements, respectively. In addition, adsorption of the bovine serum albumin was examined with fluorescence microscopy. The thermal response of wettability and morphology of the coatings followed by that of protein adsorption revealed two distinct transitions at 10 and 23 °C. For the first time, three stage switching was observed not only for surface wetting but also for morphology and protein adsorption. Moreover, the influence of the pH on thermo-sensitivity of modified surfaces was shown.
authors.affiliation: Lviv Polytechnic National University , S. Bandery 12, 79013 Lviv, Ukraine.
authors.forename: Yurij
authors.identifier: http://orcid.org/0000-0002-6498-2619
authors.initials: Y
authors.lastname: Stetsyshyn
authors.affiliation: Smoluchowski Institute of Physics, Jagiellonian University , Łojasiewicza 11, 30-348 Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Raczkowska
authors.affiliation: Lviv Polytechnic National University , S. Bandery 12, 79013 Lviv, Ukraine.
authors.forename: Ostap
authors.initials: O
authors.lastname: Lishchynskyi
authors.affiliation: Faculty of Physics and Applied Computer Science, AGH University of Science and Technology , Al. Mickiewicza 30, 30-049 Kraków, Poland.|Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology , Al. Mickiewicza 30, 30-059 Kraków, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bernasik
authors.affiliation: Lviv Academy of Commerce , Samtshuk 9, 79011 Lviv, Ukraine.
authors.forename: Andrij
authors.initials: A
authors.lastname: Kostruba
authors.affiliation: Lviv Polytechnic National University , S. Bandery 12, 79013 Lviv, Ukraine.
authors.forename: Khrystyna
authors.initials: K
authors.lastname: Harhay
authors.affiliation: Lviv Polytechnic National University , S. Bandery 12, 79013 Lviv, Ukraine.
authors.forename: Halyna
authors.initials: H
authors.lastname: Ohar
authors.affiliation: Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology , Al. Mickiewicza 30, 30-059 Kraków, Poland.
authors.forename: Mateusz M
authors.initials: MM
authors.lastname: Marzec
authors.affiliation: Smoluchowski Institute of Physics, Jagiellonian University , Łojasiewicza 11, 30-348 Kraków, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Budkowski
mesh_terms: D000327:Adsorption; D000818:Animals; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols; D011108:Polymers; D012710:Serum Albumin, Bovine; D013499:Surface Properties; D013696:Temperature; D017155:Wettability
chemical_list: D011108:Polymers; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols
keywords: LCST; POEGMA; copolymer; poly(4-vinylpyridine); protein adsorption; stimuli-responsive polymer coatings; wettability