title: Kisspeptin-10 treatment generated specific GnRH expression in cells differentiated from rhesus monkey derived Lyon NSCs.
abstract: Embryonic stem cells (ESCs) have enormous potential as novel cell-based therapies, but their effectiveness depends on stem cell differentiation and specific signaling regulators, which remain poorly understood. In this study, a kisspeptin peptide (KP-10) was used at different dosages to determine whether rhesus macaque-derived tau GFP-Lyon ES cells underwent kisspeptin-specific neuronal differentiation. It was found that KP-10 exhibited an anti-proliferative effect on the cells and led to morphological changes and cellular differentiation consistent with neuronal stem cell (NSC) development. The cells differentiated into Gonadotrophin Releasing Hormone (GnRH) neuronal-like cell types in response to the KP-10 treatment. There has been a previously observed connection between kisspeptin signaling, GnRH neurons and their dysfunction found in congenital disorders like idiopathic hypogonadotropic hypogonadism (IHH). Although therapeutics are a still a far-off goal, the formation and development of GnRH-positive neuronal-like cells following the application of KP-10 to Lyon NSC cells opens the door for future NSC-based therapies to treat specific reproductive disorders.
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China; Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan; Department of Genetics, Xuzhou Medical College, Xuzhou city, PR China; Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou city, PR China. Electronic address: tanzeelhuma@gmail.com.
authors.forename: Tanzeel
authors.initials: T
authors.lastname: Huma
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China; Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China; Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China.
authors.forename: Xintian
authors.initials: X
authors.lastname: Hu
authors.affiliation: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China.
authors.forename: Yuanye
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China.
authors.forename: Andrew
authors.initials: A
authors.lastname: Willden
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China.
authors.forename: Joshua
authors.initials: J
authors.lastname: Rizak
authors.affiliation: Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shahab
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China; Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China. Electronic address: wangzb@mail.kiz.ac.cn.
authors.forename: Zhengbo
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007006:Hypogonadism / Q000378:metabolism; D059648:Kisspeptins / Q000494:pharmacology*; D008253:Macaca mulatta; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: C106628:KISS1 protein, human; D059648:Kisspeptins; D007987:Gonadotropin-Releasing Hormone
keywords: GnRH; Lyon ES; kisspeptin; neural stem cells; proliferation