title: Chrysin as an Anti-Cancer Agent Exerts Selective Toxicity by Directly Inhibiting Mitochondrial Complex II and V in CLL B-lymphocytes.
abstract: We investigated the effect of chrysin on isolated normal and chronic lymphocytic leukemia (CLL) B-lymphocytes and their isolated mitochondria. We report that a selective and significant increase in cytotoxicity, intracellular reactive oxygen species, mitochondrial membrane potential collapse, ADP/ATP ratio, caspase 3 activation and finally apoptosis in chrysin-treated CLL B- lymphocytes. Also we determined that chrysin selectively inhibits complex II and ATPases in cancerous mitochondria. In this study we proved that the ability of chrysin to promote apoptosis in CLL B-lymphocytes performed by selectively targeting of mitochondria. Our findings may provide a potential therapeutic approach for using chrysin to target mitochondria in CLL B-lymphocytes.
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacology and Toxicology , Shahid Beheshti University of Medical Sciences , Tehran , Iran.|b Department of Pharmacology and Toxicology , School of Pharmacy, Ardabil University of Medical Science , Ardabil , Iran.|c Students Research Committee, School of Pharmacy , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Salimi
authors.affiliation: d Biotechnology Research Center, Paramedicine Faculty , Guilan University of Medical Science , Rasht , Iran.
authors.forename: Mehryar Habibi
authors.initials: MH
authors.lastname: Roudkenar
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacology and Toxicology , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Enayatollah
authors.initials: E
authors.lastname: Seydi
authors.affiliation: e Emam Hosein Educational Hospital Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Sadeghi
authors.affiliation: f Faculty of Paramedicine, Department of Laboratory Sciences , Mazandaran University of Medical Sciences , Sari , Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Mohseni
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacology and Toxicology , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Nahal
authors.initials: N
authors.lastname: Pirahmadi
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacology and Toxicology , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Jalal
authors.initials: J
authors.lastname: Pourahmad
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004354:Drug Screening Assays, Antitumor; D042963:Electron Transport Complex II / Q000037:antagonists & inhibitors*; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008565:Membrane Proteins / Q000037:antagonists & inhibitors*; D025261:Mitochondrial Proton-Translocating ATPases; D008933:Mitochondrial Swelling / Q000187:drug effects; D010085:Oxidative Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D002352:Carrier Proteins; D005419:Flavonoids; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; C043561:chrysin; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D042963:Electron Transport Complex II; C505407:CASP3 protein, human; D053148:Caspase 3; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; C020439:oligomycin sensitivity-conferring protein
keywords: CLL; Chrysin; complex II; complex V; mitochondria