title: Development of new microsatellites for the hookworm Ancylostoma caninum and analysis of genetic diversity in Brazilian populations.
abstract: Considering the great efforts towards formulating a vaccine against hookworms, and the concerns about the spread of drug resistance through hookworm populations, it is justified to study the molecular diversity and population genetic structure of these nematodes. This work had the aim to develop microsatellite markers to investigate the genetic structure and the molecular diversity of Brazilian populations of Ancylostoma caninum. Seven microsatellites markers were successfully used to characterize five Brazilian populations. These findings may contribute to a better comprehension of the ecology, patterns of transmission, drug resistances and development of immunotherapeutic strategies in hookworms.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: rabelo@icb.ufmg.br.
authors.forename: Élida Mara Leite
authors.initials: ÉML
authors.lastname: Rabelo
authors.affiliation: Curso de Ciências Biológicas, Centro de Ciências Agrárias e Biológicas - CBAB, Universidade Estadual Vale do Acaraú, Av. da Universidade 850, campus Betânia, CEP 62040-370 Sobral, Ceará, Brazil.
authors.forename: Rodrigo Rodrigues Cambraia de
authors.initials: RRC
authors.lastname: Miranda
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Luis Fernando Viana
authors.initials: LFV
authors.lastname: Furtado
authors.affiliation: HTS Laboratory, The Francis Crick Institute, 1 Midland Road NW1 1AT, London, United Kingdom.
authors.forename: Rodrigo Aparecido Fernandes
authors.initials: RAF
authors.lastname: Redondo
authors.affiliation: Department of Integrative Biology, Oregon State University, Corvallis, OR, United States.
authors.forename: Jacob Adam
authors.initials: JA
authors.lastname: Tennessen
authors.affiliation: Department of Integrative Biology, Oregon State University, Corvallis, OR, United States.
authors.forename: Michael Scott
authors.initials: MS
authors.lastname: Blouin
mesh_terms: D000722:Ancylostoma / Q000235:genetics*; D000724:Ancylostomiasis / Q000469:parasitology; D000818:Animals; D001938:Brazil; D018509:DNA, Helminth / Q000235:genetics*; D030541:Databases, Genetic; D004285:Dogs; D056426:Genetic Loci; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats
chemical_list: D018509:DNA, Helminth
keywords: Ancylostoma caninum; Genetic diversity; Microsatellite