title: Vasoactive effect of fibronectin-derived epiviosamine-1 and related peptides in quiescent and stress models.
abstract: Following thermal burn injury, plasma fibronectin degrades within the interstitium; one possible product is EVA-1, PSHISKYILRWRPK found within the FNIII1. EVA-1 ameliorates thermal burn injury progression, and binds to and enhances PDGF-BB in promoting cell metabolism, growth and survival; shorter related peptides lose these abilities. Here we study the effect of EVA-1 and shorter peptides for their vasoactivity under quiescent and stress conditions.
  
  
  
  Using the hamster cheek pouch intravital microscopy model, five EVA-1 related peptides were applied to small arterioles via micropipette (10-16-10-4mol L-1) in quiescent tissue and after defined stress: nitric oxide or heat.
  
  
  
  Peak dilation occurred with nanomolar doses (longer peptides) or below (shorter peptides), blocked by propranolol (beta-adrenergic receptor antagonist). Micromolar doses of the same peptides induced only constriction, not antagonized by phentolamine (alpha-adrenergic receptor antagonist). Scrambled variants of two peptides yielded only constriction, suggesting constriction might be due peptide charge. Each stressor caused a left shift in dilation response, blocked by carazolol.
  
  
  
  Thus, this important region of FNIII1contains sequences that have a gradation of biological functions dependent on the length of the peptide sequence, with increased efficacy for dilation following stressors.
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USA.|Department of Physiology and Biophysics, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Mary D
authors.identifier: 0000-0003-3890-2221
authors.initials: MD
authors.lastname: Frame
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Fubao
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Dewar
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USA.|Department of Dermatology, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Richard A F
authors.initials: RAF
authors.lastname: Clark
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D005353:Fibronectins / Q000378:metabolism* / Q000494:pharmacology; D006358:Hot Temperature; D000069416:Intravital Microscopy; D009569:Nitric Oxide; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D013312:Stress, Physiological; D014661:Vasoconstriction / Q000187:drug effects*; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D005353:Fibronectins; D010455:Peptides; D009569:Nitric Oxide
keywords: bioactive peptides; endothelial dysfunction; fibroblast; fibronectin