title: The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
abstract: The enteric nervous system (ENS) can undergo adaptive and reparative changes in response to physiological and pathological stimuli. These manifest primarily as alterations in the levels of active substances expressed by the enteric neuron. While it is known that mycotoxins can affect the function of the central and peripheral nervous systems, knowledge about their influence on the ENS is limited. Therefore, the aim of the present study was to investigate the influence of low doses of zearalenone (ZEN) and T-2 toxin on calcitonin gene related peptide-like immunoreactive (CGRP-LI) neurons in the ENS of the porcine descending colon using a double immunofluorescence technique. Both mycotoxins led to an increase in the percentage of CGRP-LI neurons in all types of enteric plexuses and changed the degree of co-localization of CGRP with other neuronal active substances, such as substance P, galanin, nitric oxide synthase, and cocaine- and amphetamine-regulated transcript peptide. The obtained results demonstrate that even low doses of ZEN and T-2 can affect living organisms and cause changes in the neurochemical profile of enteric neurons.
authors.affiliation: Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 13, 10-718 Olsztyn, Poland. krystyna.makowska@uwm.edu.pl.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Makowska
authors.affiliation: Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 13, 10-718 Olsztyn, Poland. kazimierz.obremski@uwm.edu.pl.
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Obremski
authors.affiliation: Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 13, 10-718 Olsztyn, Poland. lukasz.zielonka@uwm.edu.pl.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Zielonka
authors.affiliation: Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 13, 10-718 Olsztyn, Poland. slawomir.gonkowski@uwm.edu.pl.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Gonkowski
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D044683:Colon, Descending / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D005260:Female; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D013552:Swine; D013605:T-2 Toxin / Q000633:toxicity*; D050494:Vesicular Acetylcholine Transport Proteins / Q000378:metabolism; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D050494:Vesicular Acetylcholine Transport Proteins; D013373:Substance P; D015025:Zearalenone; D052248:Nitric Oxide Synthase Type I; D013605:T-2 Toxin; D015740:Calcitonin Gene-Related Peptide
keywords: CGRP; ENS; T-2 toxin; descending colon; pig; zearalenone