title: Retention of Ag-specific memory CD4+T cells in the draining lymph node indicates lymphoid tissue resident memory populations.
abstract: Several different memory T-cell populations have now been described based upon surface receptor expression and migratory capabilities. Here we have assessed murine endogenous memory CD4+T cells generated within a draining lymph node and their subsequent migration to other secondary lymphoid tissues. Having established a model response targeting a specific peripheral lymph node, we temporally labelled all the cells within draining lymph node using photoconversion. Tracking of photoconverted and non-photoconverted Ag-specific CD4+T cells revealed the rapid establishment of a circulating memory population in all lymph nodes within days of immunisation. Strikingly, a resident memory CD4+T cell population became established in the draining lymph node and persisted for several months in the absence of detectable migration to other lymphoid tissue. These cells most closely resembled effector memory T cells, usually associated with circulation through non-lymphoid tissue, but here, these cells were retained in the draining lymph node. These data indicate that lymphoid tissue resident memory CD4+T-cell populations are generated in peripheral lymph nodes following immunisation.
authors.affiliation: Institute of Immunology & Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: Clare L
authors.initials: CL
authors.lastname: Marriott
authors.affiliation: Institute of Immunology & Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: Emma E
authors.initials: EE
authors.lastname: Dutton
authors.affiliation: Laboratory of Immunology, Faculty of Pharmacy, Osaka-Ohtani University 3-11-1 Nishikiorikita, Tondabayashi-city, Osaka prefecture, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Tomura
authors.affiliation: Institute of Immunology & Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: David R
authors.identifier: 0000-0003-3757-7594
authors.initials: DR
authors.lastname: Withers
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology; D007114:Immunization; D007156:Immunologic Memory; D008198:Lymph Nodes / Q000033:anatomy & histology / Q000166:cytology / Q000276:immunology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology*; D051379:Mice; D016176:T-Lymphocyte Subsets / Q000276:immunology
keywords: Cell trafficking; Lymph nodes; Memory CD4+ T cells; Memory cells