title: An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon.
abstract: Persimmon fruits accumulate a large amount of proanthocyanidins (PAs) during development. PAs cause a dry or puckering sensation due to its astringency. Pollination constant and non-astringent (PCNA) persimmon fruits can lose astringency during fruit ripening. However, little is known about the mechanism of natural de-astringency of Chinese PCNA (CPCNA). To gain insight into the molecular events of CPCNA natural de-astringency, we used mRNA-seq and iTRAQ-based quantitative proteomic analysis to measure changes in genes and proteins expression at two key stages of natural astringency removal (i.e. 10 and 20 weeks after bloom) and water-treated (i.e. 40 °C·12 h) de-astringency fruits. Our analyses show that the three predominantly process in CPCNA de-astringency: (1) water treatment strongly up-regulates glycolysis/acetaldehyde metabolism, (2) expression of genes/proteins involved in PA biosynthetic pathway was remarkably reduced in natural and water-treated de-astringency, (3) sugar metabolism and ethylene related pathway were quite abundant in natural de-astringency. We also found ethylene-related TFs were quite abundant in natural de-astringency, followed by WRKY and NAC transcription factors. These results provide an initial understanding of the predominantly biological processes underlying the natural de-astringency and "coagulation effect" in CPCNA.
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Chen
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Yalou
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.|Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang 438000, Hubei, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Luo
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D029788:Diospyros / Q000235:genetics* / Q000254:growth & development; D015536:Down-Regulation / Q000235:genetics; D005638:Fruit / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D006019:Glycolysis; D008954:Models, Biological; D054817:Pollination / Q000235:genetics*; D044945:Proanthocyanidins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015203:Reproducibility of Results; D012995:Solubility; D013634:Tannins / Q000032:analysis; D014157:Transcription Factors / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics; D014867:Water
chemical_list: D044945:Proanthocyanidins; D020543:Proteome; D013634:Tannins; D014157:Transcription Factors; D014867:Water; D000079:Acetaldehyde