title: Inhibition of ectopic microtubule assembly by the kinesin-13 KLP-7 prevents chromosome segregation and cytokinesis defects in oocytes.
abstract: In most species, oocytes lack centrosomes. Accurate meiotic spindle assembly and chromosome segregation - essential to prevent miscarriage or developmental defects - thus occur through atypical mechanisms that are not well characterized. Using quantitativein vitroandin vivofunctional assays in theC. elegansoocyte, we provide novel evidence that the kinesin-13 KLP-7 promotes destabilization of the whole cellular microtubule network. By counteracting ectopic microtubule assembly and disorganization of the microtubule network, this function is strictly required for spindle organization, chromosome segregation and cytokinesis in meiotic cells. Strikingly, when centrosome activity was experimentally reduced, the absence of KLP-7 or the mammalian kinesin-13 protein MCAK (KIF2C) also resulted in ectopic microtubule asters during mitosis inC. eleganszygotes or HeLa cells, respectively. Our results highlight the general function of kinesin-13 microtubule depolymerases in preventing ectopic, spontaneous microtubule assembly when centrosome activity is defective or absent, which would otherwise lead to spindle microtubule disorganization and aneuploidy.
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, Paris F-75205, France.
authors.forename: Emmanuelle
authors.identifier: 0000-0001-9098-0994
authors.initials: E
authors.lastname: Gigant
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, Paris F-75205, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Stefanutti
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, Paris F-75205, France.
authors.forename: Kimberley
authors.initials: K
authors.lastname: Laband
authors.affiliation: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
authors.forename: Agata
authors.initials: A
authors.lastname: Gluszek-Kustusz
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, Paris F-75205, France.
authors.forename: Frances
authors.initials: F
authors.lastname: Edwards
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, Paris F-75205, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Lacroix
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, Paris F-75205, France.
authors.forename: Gilliane
authors.initials: G
authors.lastname: Maton
authors.affiliation: Columbia University, Department of Pathology and Cell Biology, New York, NY 10032, USA.
authors.forename: Julie C
authors.initials: JC
authors.lastname: Canman
authors.affiliation: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
authors.forename: Julie P I
authors.initials: JPI
authors.lastname: Welburn
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cité, Paris F-75205, France julien.dumont@ijm.fr.
authors.forename: Julien
authors.identifier: 0000-0001-5312-9770
authors.initials: J
authors.lastname: Dumont
mesh_terms: D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D020090:Chromosome Segregation; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D016547:Kinesins / Q000378:metabolism*; D008540:Meiosis; D008870:Microtubules / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C502821:klp-7 protein, C elegans; D016547:Kinesins
keywords: Chromosome segregation; Cytoskeleton; Meiotic spindle; Microtubule depolymerase; Microtubule dynamics; Polar body extrusion