title: Sodium houttuyfonate inhibits LPS-induced inflammatory response via suppressing TLR4/NF-ĸB signaling pathway in bovine mammary epithelial cells.
abstract: Mastitis is an inflammatory reaction caused by microorganisms in the mammary gland which usually leads to the decrease of the dairy production. It vastly makes bad effect on the cattle industry all over the world. Nowadays, an increasing number of scientists keep a watchful eye on natural compounds to prevent mastitis. Sodium houttuyfonate (SH) has been reported to have antibacterial and anti-inflammatory effects. This experiment aimed to investigate the anti-inflammatory properties of SH on LPS-stimulated primary bovine mammary epithelial cells (bMEC). The effects of SH on TNF-α, IL-1β, and IL-6 production were detected by qRT-PCR. Western blot analysis was used for detecting the effects of SH on TLR4/NF-κB signal pathways. The results showed that SH significantly inhibited LPS-stimulated TNF-α, IL-1β and IL-6 production. Furthermore, SH significantly inhibited LPS-induced TLR4 expression and NF-ĸB activation. In summary, these results suggested that SH inhibited LPS-induced inflammatory response by inhibiting TLR4/NF-ĸB signaling pathway. SH is a potential agent for the treatment of mastitis.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, People's Republic of China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, People's Republic of China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shen
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, People's Republic of China.
authors.forename: Naisheng
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, People's Republic of China. Electronic address: fuyunhesky@sina.com.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Fu
mesh_terms: D000473:Alkanes / Q000037:antagonists & inhibitors*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000187:drug effects; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000187:drug effects; D008414:Mastitis, Bovine / Q000188:drug therapy; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D013447:Sulfites / Q000037:antagonists & inhibitors*; D051197:Toll-Like Receptor 4 / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000187:drug effects
chemical_list: D000473:Alkanes; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D013447:Sulfites; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C473296:sodium houttuyfonate
keywords: Bovine mammary epithelial cells; LPS; TLR4