title: Proteomic analysis of Taenia ovis metacestodes by high performance liquid chromatography-coupled tandem mass spectrometry.
abstract: Taenia ovis metacestodes reside in the muscle of sheep and goats, and may cause great economic loss due to condemnation of carcasses if not effectively controlled. Although advances have been made in the control of T. ovis infection, our knowledge of T. ovis biology is limited. Herein the protein profiling of T. ovis metacestodes was determined by liquid chromatography-linked tandem mass spectrometry. A total of 966 proteins were identified and 25.1% (188/748) were annotated to be associated with metabolic pathways. Consistently, GO analysis returned a metabolic process (16.27%) as one of two main biological process terms. Moreover, it was found that 24 proteins, including very low-density lipoprotein receptor, enolase, paramyosin and endophilin B1, were abundant in T. ovis metacestodes. These proteins may be associated with motility, metabolism, signaling, stress, drug resistance and immune responses. Furthermore, comparative analysis of 5 cestodes revealed the presence of Taenia-specific enolases. These data provide clues for better understanding of T. ovis biology, which is informative for effective control of infection.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: zhengyadong@caas.cn.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D015801:Helminth Proteins / Q000235:genetics*; D007814:Larva / Q000235:genetics; D020543:Proteome / Q000235:genetics*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D013621:Taenia / Q000235:genetics* / Q000254:growth & development; D013622:Taeniasis / Q000469:parasitology / Q000662:veterinary; D053719:Tandem Mass Spectrometry / Q000662:veterinary
chemical_list: D015801:Helminth Proteins; D020543:Proteome
keywords: Metacestode; Proteome; Taenia ovis