title: TSPAN12 promotes chemoresistance and proliferation of SCLC under the regulation of miR-495.
abstract: Studies have shown that TSPAN12 serves as an oncogenic gene or tumor suppressor depending on the types of cancer. However, its role in the drug resistance of small cell lung cancer (SCLC) is still unknown. In this study, we investigated whether TSPAN12 regulates chemoresistance, proliferation and tumor growth of SCLC under the regulation of miR-495 using two drug resistant cell lines. The results showed that down-regulation of TSPAN12 inhibited cells chemoresistance, proliferation and tumor growth. Besides, TSPAN12 elevation in SCLC specimens correlated with poor pathologic stage and shorter survival time. In addition, the dual luciferase assay indicated that TSPAN12 was one of the directly targeted genes of miR-495. Our study revealed that TSPAN12 promoted chemoresistance of SCLC under the regulation of miR-495. TSPAN12 depletion is a promising strategy for inhibiting the chemoresistance in SCLC.
authors.affiliation: Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. Electronic address: 296698541@qq.com.
authors.forename: Minting
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. Electronic address: weitingyouyou@qq.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.affiliation: Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. Electronic address: 849761457@qq.com.
authors.forename: Qiongyao
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pathology, Guangdong Medical University, China. Electronic address: 99664310@qq.com.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of Xi'an JiaoTong University, Xi'an, Shanxi, China. Electronic address: 779673168@qq.com.
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Tang
authors.affiliation: Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. Electronic address: linlangg@yahoo.com.
authors.forename: Linlang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. Electronic address: zhuwl2017@163.com.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000368:Aged; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D055752:Small Cell Lung Carcinoma / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016019:Survival Analysis; D059470:Tetraspanins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; C577768:MIRN495 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C545100:TSPAN12 protein, human; D059470:Tetraspanins; D008156:Luciferases; D002945:Cisplatin
keywords: Chemoresistance; SCLC; TSPAN12; miR-495