title: Proteomics analysis reveals the molecular mechanism underlying the transition from primary to secondary growth of poplar.
abstract: Wood is the most important natural source of energy and also provides fuel and fiber. Considering the significant role of wood, it is critical to understand how wood is formed. Integration of knowledge about wood development at the cellular and molecular levels will allow more comprehensive understanding of this complex process. In the present study, we used a comparative proteomic approach to investigate the differences in protein profiles between primary and secondary growth in young poplar stems using tandem mass tag (TMT)-labeling. More than 10,816 proteins were identified, and, among these, 3106 proteins were differentially expressed during primary to secondary growth. Proteomic data were validated using a combination of histochemical staining, enzyme activity assays, and quantitative real-time PCR. Bioinformatics analysis revealed that these differentially expressed proteins are related to various metabolic pathways, mainly including signaling, phytohormones, cell cycle, cell wall, secondary metabolism, carbohydrate and energy metabolism, and protein metabolism as well as redox and stress pathways. This large proteomics dataset will be valuable for uncovering the molecular changes occurring during the transition from primary to secondary growth. Further, it provides new and accurate information for tree breeding to modify wood properties.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, No. 26 Hexing Road, Harbin 150040, China. Electronic address: liy080822@ibcas.ac.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing 100093, China. Electronic address: jinfeng@ibcas.ac.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing 100093, China. Electronic address: chaoqing@ibcas.ac.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chao
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing 100093, China. Electronic address: wangbc@ibcas.ac.cn.
authors.forename: Bai-Chen
authors.initials: BC
authors.lastname: Wang
mesh_terms: D019295:Computational Biology; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D032107:Populus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D010940:Plant Proteins
keywords: Poplar; Primary growth; Proteomics; Secondary growth; TMT