title: Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality.
abstract: Novel, hybrid fibrinogen/polylactic acid (FBG/PLA) nanofibers with different configuration (random vs aligned) and dimensionality (2-D vs 3-D environment) were used to control the overall behavior and the osteogenic differentiation of human adipose-derived mesenchymal stem cells (ADMSCs). Aligned nanofibers in both the 2-D and 3-D configurations are proved to be favored for osteodifferentiation. Morphologically, we found that on randomly configured nanofibers, the cells developed a stellate-like morphology with multiple projections; however, time-lapse analysis showed significantly diminished cell movements. Conversely, an elongated cell shape with advanced cell spreading and extended actin cytoskeleton accompanied with significantly increased cell mobility were observed when cells attached on aligned nanofibers. Moreover, a clear tendency for higher alkaline phosphatase activity was also found on aligned fibers when ADMSCs were switched to osteogenic induction medium. The strongest accumulation of Alizarin red (AR) and von Kossa stain at 21 days of culture in osteogenic medium were found on 3-D aligned constructs while the rest showed lower and rather undistinguishable activity. Quantitative reverse transcription-polymerase chain reaction analysis for Osteopontin (OSP) and RUNX 2 generally confirmed this trend showing favorable expression of osteogenic genes activity in 3-D environment particularly in aligned configuration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2065-2074, 2017.
authors.affiliation: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors.forename: Dencho
authors.initials: D
authors.lastname: Gugutkov
authors.affiliation: Department of Orthopaedic Surgery, Experimental Orthopaedics, Medical University Innsbruck, Innrain 36, Innsbruck, Austria.
authors.forename: Firas
authors.initials: F
authors.lastname: Awaja
authors.affiliation: Tissue Bank BulGen, Str. Hristo Blagoev 25, Sofia, 1330, Bulgaria.
authors.forename: Kalina
authors.initials: K
authors.lastname: Belemezova
authors.affiliation: Institute for Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Milena
authors.initials: M
authors.lastname: Keremidarska
authors.affiliation: Institute for Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Natalia
authors.initials: N
authors.lastname: Krasteva
authors.affiliation: Tissue Bank BulGen, Str. Hristo Blagoev 25, Sofia, 1330, Bulgaria.
authors.forename: Stanimir
authors.initials: S
authors.lastname: Kyurkchiev
authors.affiliation: Center for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Valencia, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: Gallego-Ferrer
authors.affiliation: Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara, Turkey.
authors.forename: Sukran
authors.initials: S
authors.lastname: Seker
authors.affiliation: Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara, Turkey.
authors.forename: Ayşe Eser
authors.initials: AE
authors.lastname: Elçin
authors.affiliation: Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara, Turkey.
authors.forename: Yaşar Murat
authors.initials: YM
authors.lastname: Elçin
authors.affiliation: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.|ICREA (Institucio Catalana de Recerca i Estudis Avançats), Barcelona, Spain.
authors.forename: George
authors.initials: G
authors.lastname: Altankov
mesh_terms: D002454:Cell Differentiation; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*
chemical_list: D011091:Polyesters; C033616:poly(lactide); D005340:Fibrinogen
keywords: cell movements; fibrinogen; mesenchymal stem cells; nanofibers; osteogenic