title: UVA radiation augments cytotoxic activity of psoralens in melanoma cells.
abstract: Melanoma is an aggressive form of skin cancer. The aim of the study was to evaluate the influence of UVA radiation and psoralens: 5-methoxypsoralen (5-MOP) or 8-methoxypsoralen (8-MOP) on melanoma cells viability.
  
  
  
  The amelanotic C32 and melanotic COLO829 human melanoma cell lines were exposed to increasing concentrations of psoralens (0.1-100 μM) in the presence or absence of UVA radiation. Cell viability was evaluated by the WST-1 assay.
  
  
  
  We demonstrated that 8-MOP, in contrast to 5-MOP, has no cytotoxic effect on both melanoma cell lines. Simultaneous exposure of cells to 8-MOP and UVA radiation caused significant cytotoxic response in C32 cells where the EC50value was estimated to be 131.0 μM (UVA dose: 1.3 J/cm2) and 105.3 μM (UVA dose: 2.6 J/cm2). The cytotoxicity of 5-MOP on both C32 and COLO829 cells was significantly augmented by UVA radiation - the EC50was estimated to be 22.7 or 7.9 μM (UVA dose: 1.3 J/cm2) and 24.2 or 7.0 μM (UVA dose: 2.6 J/cm2), respectively.
  
  
  
  The demonstrated high cytotoxic response after simultaneous exposure of melanoma cells to psoralens and UVA radiation in vitro suggests the usefulness of PUVA therapy to treat melanoma in vivo.
authors.affiliation: a Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Wrześniok
authors.affiliation: a Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Beberok
authors.affiliation: a Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Rok
authors.affiliation: a Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Delijewski
authors.affiliation: b Student Scientific Society at the Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Hechmann
authors.affiliation: b Student Scientific Society at the Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Oprzondek
authors.affiliation: a Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Zuzanna
authors.initials: Z
authors.lastname: Rzepka
authors.affiliation: c Department of Pharmaceutical Botany and Herbal Medicine, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Bacler-Żbikowska
authors.affiliation: a Department of Pharmaceutical Chemistry , Medical University of Silesia in Katowice , Sosnowiec , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Buszman
mesh_terms: D000078223:5-Methoxypsoralen; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004357:Drug Synergism; D006801:Humans; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology*; D008730:Methoxsalen / Q000008:administration & dosage / Q000031:analogs & derivatives; D011701:PUVA Therapy / Q000379:methods*; D011879:Radiotherapy Dosage; D016896:Treatment Outcome
chemical_list: D000078223:5-Methoxypsoralen; D008730:Methoxsalen
keywords: 5-methoxypsoralen; 8-methoxypsoralen; Melanoma; UVA radiation; cell viability