title: Antinociceptive pharmacological profile of Dysphania graveolens in mouse.
abstract: This work evaluates the potential antinociceptive activity of Dysphania graveolens, traditional medicinal plant used in Mexico to treat stomach pain. A CH2Cl2-MeOH extract, infusion and essential oil from aerial parts of Dysphania graveolens were evaluated in hot plate and writhing tests in mice. The metabolites pinostrobin, pinocembrin and chrysin were isolated from the Dysphania graveolens infusion; next, they were evaluated in both nociceptive tests. To confirm the antinociceptive activity and explore the possible participation of opioid, GABA and serotonin receptors in the pharmacological mechanism, a formalin test was used. Oral administration of Dysphania graveolens CH2Cl2-MeOH extract, infusion and essential oil (31-316mg/kg) produced an antinociceptive response to thermic and chemical algesic stimuli. Essential oil was the most active partition of this plant. In addition, the secondary metabolites pinostrobin, pinocembrin and chrysin possess a significant antinociceptive effect. This response was confirmed by the formalin test for the CH2Cl2-MeOH extract of Dysphania graveolens and chrysin. In both cases, the antinociceptive activity was reverted in the presence of naltrexone, flumazenil and bicuculline antagonists. The 5-HT2A/2Creceptors did not participate in the antinociceptive response of this plant. The overall information tends to support the efficacy of Dysphania graveolens as an analgesic and its cultural use in abdominal pain.
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Ciudad de México 11340, México. Electronic address: myrnadeciga@hotmail.com.
authors.forename: Myrna
authors.initials: M
authors.lastname: Déciga-Campos
authors.affiliation: Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, México. Electronic address: rachel@unam.mx.
authors.forename: Rachel
authors.initials: R
authors.lastname: Mata
authors.affiliation: Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, México. Electronic address: riveroic@unam.mx.
authors.forename: Isabel
authors.initials: I
authors.lastname: Rivero-Cruz
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry*; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000517:prevention & control; D010147:Pain Measurement / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D010936:Plant Extracts
keywords: Antinociception; Chrysin; Dysphania graveolens; Epazote; Flavonoids