title: Thermosensitive porphyrin-incorporated hydrogel with four-arm PEG-PCL copolymer (II): doxorubicin loaded hydrogel as a dual fluorescent drug delivery system for simultaneous imaging tracking in vivo.
abstract: Visualization of a drug delivery system could reveal the pharmacokinetic properties, which is essential for the design of a novel drug delivery system. In vivo optical imaging offers an advanced tool to monitor the drug release process and the therapeutic effect by the combination of fluorescence imaging and bioluminescence imaging. Multispectral fluorescence imaging can separate the drug and the carrier without interference. Herein, a dual fluorescent anti-tumor drug delivery system was monitored with the doxorubicin-loaded hydrogel to further explore the application of the porphyrin-incorporated hydrogel with four-arm PEG-PCL copolymer as a drug carrier, based on the beneficial fluorescence and good biocompatibility of the porphyrin incorporated hydrogel. Using nude mice bearing luciferase expressed hepatic tumor as models, the whole process from the drug delivery to the tumor therapeutic effects were real time visualized simultaneously after administration at interval from 0 to 18 d. The imaging results suggest that the fluorescence signals of the drug and the carrier can be separated and unmixed from the drug-loaded hydrogel successfully, avoiding the interference of the fluorescence signals. The tumor growth or inhibition can be real time tracked and analyzed quantitatively by bioluminescence imaging. Noninvasive continuous tracking the in vivo drug delivery process simultaneously is a potential trend for the precise drug delivery and treatment.
authors.affiliation: a Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin , PR China and.
authors.forename: Xia
authors.initials: X
authors.lastname: Dong
authors.affiliation: b School of Life Science and Technology, Xinxiang Medical University , Xinxiang , Henan , PR China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Chen
authors.affiliation: b School of Life Science and Technology, Xinxiang Medical University , Xinxiang , Henan , PR China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Qin
authors.affiliation: a Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin , PR China and.
authors.forename: Chang
authors.initials: C
authors.lastname: Wei
authors.affiliation: a Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin , PR China and.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: a Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin , PR China and.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Liu
authors.affiliation: a Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin , PR China and.
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
authors.affiliation: a Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin , PR China and.
authors.forename: Feng
authors.initials: F
authors.lastname: Lv
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers; D004339:Drug Compounding; D005456:Fluorescent Dyes / Q000737:chemistry*; D020100:Hydrogels; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D061848:Optical Imaging / Q000379:methods*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D005456:Fluorescent Dyes; D020100:Hydrogels; D011091:Polyesters; D011166:Porphyrins; C016240:polycaprolactone; D011092:Polyethylene Glycols; D004317:Doxorubicin; D008156:Luciferases
keywords: Imaging tracking; drug delivery; hydrogel; multispectral fluorescence; porphyrin