title: Phomopsis longicolla RNA virus 1 - Novel virus at the edge of myco- and plant viruses.
abstract: The complete nucleotide sequence of a new RNA mycovirus in the KY isolate of Phomopsis longicolla Hobbs 1985 and its protoplasts subcultures p5, p9, and ME711 was discovered. The virus, provisionally named Phomopsis longicolla RNA virus 1 (PlRV1), was localized in mitochondria and was determined to have a genome 2822 nucleotides long. A single open reading frame could be translated in silico by both standard and mitochondrial genetic codes into a product featuring conservative domains for an RNA-dependent RNA polymerase (RdRp). The RdRp of PlRV1 has no counterpart among mycoviruses, but it is about 30% identical with the RdRp of plant ourmiaviruses. Recently, new mycoviruses related to plant ourmiaviruses and forming one clade with PlRV1 have been discovered. This separate clade could represent the crucial link between plant and fungal viruses.
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic; Department of Genetics, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 31a, 370 05 České Budějovice, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Hrabáková
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Igor
authors.initials: I
authors.lastname: Koloniuk
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Petrzik
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000821:virology*; D001483:Base Sequence; D000067148:Fungal Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: Mitovirus; Ourmiavirus; Ourmycovirus; Phomopsis longicolla; Phytopathogenic fungus; RNA mycovirus