title: Electrochemical Lateral Flow Paper Strip for Oxidative-Stress Induced DNA Damage Assessment.
abstract: The phrase "oxidative-stress induced DNA damage" is commonly used in both the scientific literature and common media outlets, and is frequently linked to detrimental elements of aging as well as the onset of illnesses. Due to the growing focus on this topic, a clear need has emerged to develop a quantitative, low-cost methodology to allow for periodic monitoring of oxidative-stress induced DNA damage within individuals. Recent literature examining the link between oxidative stress and the onset of various cancers has made monitoring an even more pressing need. The mechanism of oxidative-stress induced DNA damage originates in chronic inflammation, which in turn activates various transcription factors and diseases that influence the onset of tumor development, chemoresistance, radioresistance, and other harmful cellular processes. While current technologies that aim to provide quantitative metrics require extremely expensive equipment and significant technical expertise, our laboratory has designed a low-cost methodology utilizing a combination of carbon nanotubes, paper electrodes, and immunochromatographic strips.
authors.affiliation: Nanobioengineering & Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL, 33174, USA.
authors.forename: Jared
authors.initials: J
authors.lastname: Leichner
authors.affiliation: Nanobioengineering & Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL, 33174, USA.
authors.forename: Mehenur
authors.initials: M
authors.lastname: Sarwar
authors.affiliation: Nanobioengineering & Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL, 33174, USA.
authors.forename: Amirali
authors.initials: A
authors.lastname: Nilchian
authors.affiliation: Nanobioengineering & Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL, 33174, USA.
authors.forename: Xuena
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing, 100875, China.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, China.
authors.forename: Shaomin
authors.initials: S
authors.lastname: Shuang
authors.affiliation: Nanobioengineering & Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL, 33174, USA. licz@fiu.edu.|School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, China. licz@fiu.edu.
authors.forename: Chen-Zhong
authors.initials: CZ
authors.lastname: Li
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D037742:Nanotubes, Carbon; D018384:Oxidative Stress; D010209:Paper; D017382:Reactive Oxygen Species; D011934:Reagent Strips
chemical_list: D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D011934:Reagent Strips; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-OHdG; Cancer; Carbon nanotubes; Chronoamperometric; Colorimetric; DNA oxidative damage; Lateral flow immunosensor; Paper strip; ROS