title: Involvement of PKA signalling in anti-inflammatory effects of chitosan oligosaccharides in IPEC-J2 porcine epithelial cells.
abstract: Weaning is characterized by intestinal inflammation, which is a big challenge in pig industry. Control of intestinal inflammation is important for improvement of growth performance and health. Therefore, the study was focused on the anti-inflammatory activity of low-molecular-weight chitosan oligosaccharide (LCOS) in a porcine small intestinal epithelial cell line (IPEC-J2). The results showed that TNF-α, as inflammation inducer, significantly upregulated the mRNA expression of IL-8 and MCP-1. Afterwards, LCOS significantly attenuated mRNA expression of IL-8 and MCP-1 induced by TNF-α in the cells. Mannose (MAN), as ligand of mannose receptor, had no effect on the anti-inflammatory activity of LCOS, which suggested that mannose receptor may not involve in the anti-inflammatory activity of LCOS in IPEC-J2 cells. Interestingly, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide 2HCl hydrate (H89), as PKA (protein kinase A)-specific inhibitor, reversed the mRNA expression of IL-8 when co-cultured with LCOS. Furthermore, LCOS concentration dependent downregulated the mRNA expression of claudin-1 compared with TNF-α treatment. However, the trans-epithelial electric resistance (TEER) was not affected by LCOS when co-cultured with TNF-α in 3 hr. In conclusion, LCOS have a potent anti-inflammatory activity, and as a feed additives, may be useful for the inhibition of inflammatory process in weaning period of pigs with intestinal inflammation occurring.
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: J W
authors.initials: JW
authors.lastname: Yang
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: G
authors.initials: G
authors.lastname: Tian
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: D W
authors.initials: DW
authors.lastname: Chen
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: P
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: X B
authors.initials: XB
authors.lastname: Mao
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Huang
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
authors.forename: B
authors.identifier: http://orcid.org/0000-0001-6605-6742
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D007413:Intestinal Mucosa / Q000166:cytology*; D008070:Lipopolysaccharides / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013552:Swine
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D048271:Chitosan; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: IPEC-J2 cells; chitosan oligosaccharide; inflammation