title: An integrated strategy using UPLC-QTOF-MSEand UPLC-QTOF-MRM (enhanced target) for pharmacokinetics study of wine processed Schisandra Chinensis fructus in rats.
abstract: Currently the pharmacokinetic (PK) research of herbal medicines is still limited and facing critical technical challenges on quantitative analysis of multi-components from biological matrices which often accompanied by lacking of authentic standards and low concentration. This present work contributes to the development of an integrated strategy for extensive pharmacokinetics assessments, and a selective and sensitive method independent of authentic standards for multi-components analysis based on the use of ultra-performance liquid chromatography/quadrupole-time-of-flight/MSE(UPLC-TOF-MSE) and UPLC-TOF-MRM (rnhanced target). Initially, phytochemicals were identified by UPLC-TOF-MSEanalysis, subsequently the identified components were matched with authentic standards and pre-classified, and UPLC-QTOF-MRM method optimized and developed. To guarantee reliable results, three rules are necessary: (1) detection with a mass error of less than 5ppm; (2) same class chemical compositions with structural high similarity between analytes with and without authentic reference substance; (3) a matching retention time between TOF-MRM mode and TOF-MSEwithin 0.2min. The developed and validated method was applied for the simultaneous determination of 12 lignans in rat plasma after administered with wine processed Schisandra Chinensis fructus (WPSCF) extract. Such an approach was found capable of providing extensive pharmacokinetic profiles of multi-components absorbed into blood after oral administrated with WPSCF extract. The results also indicated that significant difference in pharmacokinetics parameters of dibenzocyclooctadiene lignans was observed between schizandrin and gomisin compounds. For lignans, the absorption via gastrointestinal tract were all rapid and maintained relatively long retention time, especially for schisantherin A and schisantherin B with higher plasma exposure.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Kuangyi
authors.initials: K
authors.lastname: Liu
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Yonggui
authors.initials: Y
authors.lastname: Song
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Mi
authors.initials: M
authors.lastname: Peng
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Hanyun
authors.initials: H
authors.lastname: Li
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Li
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Bingwei
authors.initials: B
authors.lastname: Feng
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Xu
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China. Electronic address: sud94@aliyun.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Su
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D032141:Schisandra / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014920:Wine / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Herbal medicine; Pharmacokinetics; QTOF-MRM; QTOF-MS(E); Wine processed Schisandra Chinensis fructus