title: Oxidative modifications of blood serum proteins in myasthenia gravis.
abstract: Myasthenia gravis (MG) is an autoimmune disease caused by production of antibodies against acetylcholine receptors of the neuromuscular junction (Ab). The aim of this study was to ascertain if oxidative stress accompanies MG by estimation of the several independent parameters of oxidative damage, mainly the levels of oxidative modifications of blood serum proteins. The group studied consisted of 50 MG patients (28 females and 22 males), 24 with ocular MG (OMG) and 26 with generalized MG (GMG), of mean age of 66.7 (30-81)years (y), mean disease duration of 9.5 (0.5-34)y, mean level of Ab of 8.9 (0.1-85)nmol/ml, and 25 age-matched healthy controls. MG patients were stratified into groups according to disease duration (<5y or >5y), Ab level (low, <3 or high, >3nmol/l) as well as symptoms (GMG or OMG). Glycophore fluorescence was increased in OMGa. Dityrosine was increased in both types of MGc, in patients ill <5band >5cy, with lowcand highclevels of Ab. N-formylkynurenine was increased in OMGaand GMGb, in both disease duration groupsa, in the group of low Aba. Kynurenine was increased in the group with high Aba. Tryptophan fluorescence was decreased in OMGband GMGc, in patients ill for <5band >5ay, with lowaand highcAb. Serum thiol group concentration were decreased in GMGc, in patients ill for >5yb. AOPP level was elevated in OMGa, in patients ill for >5yawith high Aba. Protein carbonyls were increased in both OMGcand GMGc, in patients ill for >5ay, with lowband highbAb. FRAP and ABTS•scavenging by fast antioxidants were unchanged, but ABTS•scavenging by slow antioxidants was lower in OMGband GMGc, in patients ill for >5cy, in patients with lowcand highbAb (ap<0.05,bp<0.01,cp<0.001). These results demonstrate systemic oxidative stress in MG, suggesting therapeutic use of antioxidants.
authors.affiliation: Department of Neurology in Zabrze, Medical University of Silesia, 3-go Maja St. 13-15, Zabrze 41-800, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Adamczyk-Sowa
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Zelwerowicza St. 4, 35-601 Rzeszów, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Bieszczad-Bedrejczuk
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Zelwerowicza St. 4, 35-601 Rzeszów, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Galiniak
authors.affiliation: Department of Neurology in Zabrze, Medical University of Silesia, 3-go Maja St. 13-15, Zabrze 41-800, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Rozmiłowska
authors.affiliation: Department of Thoracic Surgery, SMDZ in Zabrze, Medical University of Silesia, 3-go Maja St. 13-15, Zabrze 41-800, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Czyżewski
authors.affiliation: Department of Thoracic Surgery, SMDZ in Zabrze, Medical University of Silesia, 3-go Maja St. 13-15, Zabrze 41-800, Poland; Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska St. 141/143, 90-236 Lodz, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Zelwerowicza St. 4, 35-601 Rzeszów, Poland. Electronic address: isadowska@poczta.fm.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sadowska-Bartosz
mesh_terms: D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood; D052160:Benzothiazoles / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D007737:Kynurenine / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009157:Myasthenia Gravis / Q000097:blood* / Q000601:surgery; D018384:Oxidative Stress / Q000502:physiology*; D050050:Protein Carbonylation; D011950:Receptors, Cholinergic / Q000276:immunology; D013451:Sulfonic Acids / Q000378:metabolism; D013934:Thymectomy; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D062107:Advanced Oxidation Protein Products; D001323:Autoantibodies; D052160:Benzothiazoles; D001798:Blood Proteins; D006021:Glycophorins; D011950:Receptors, Cholinergic; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D007737:Kynurenine; C002744:3-nitrotyrosine; D014443:Tyrosine
keywords: AOPP; Myasthenia gravis; Oxidative stress; Protein carbonylation; Protein glycoxidation; Serum total antioxidant capacity