title: The specification of metameric order in the insectCallosobruchus maculatus Fabr. (Coleoptera) : II. The effects of temporary constriction on segment number.
abstract: Mechanically dividing an insect egg into anterior and posterior fragments results in a segment gap (Sander 1976), a loss of non-terminal segments in the constricted region. By altering the stage and duration of constriction, we produced different types of egg fragments in the pea beetleCallosobruchus. The patterns formed by these fragments suggest the existence of interactions between anterior and posterior egg regions that influence segment patterning and placement. Segments in excess of the numbers expected on the basis of permanent constrictions were produced in fragments when: (1) the constriction was released before cellularization occurred and (2) in addition the complementary fragment degenerated. Apparently the degenerating fragment induced the formation of excess segments in the developing fragment. Differences in the time and extent of excess segment formation in anterior versus posterior fragments suggest an asymmetric distribution of prerequisites for segment formation. This conclusion is consistent with our finding that a partial reversal of segment sequence (double abdomen formation) can be induced only in posterior fragments by a degenerating fragment, but not in anterior fragments (see companion paper).The formation of excess segments shows that the segment gap observed after permanent separation cannot be due to non-specific damage, caused by the process of constriction as such, to the egg or to localized putative segment precursors.
authors.affiliation: Department of Zoology, Catholic University, Toernooiveld, Nijmegen, The Netherlands.|Department of Zoology, Duke University, Durham, North Carolina, USA.
authors.forename: Jitse Michiel
authors.initials: JM
authors.lastname: van der Meer
authors.affiliation: Department of Zoology, Catholic University, Toernooiveld, Nijmegen, The Netherlands.|Department of Zoology, Duke University, Durham, North Carolina, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Miyamoto
keywords: Egg constriction; Gap phenomenon; Insect embryogenesis; Interaction between egg regions; Segment pattern formation