title: Live cell imaging of bacterial cells: Pyrenoylpyrrole-based fluorescence labeling.
abstract: A novel substituted pyrenoylpyrroles was synthesized by the reaction of pyrenoyl chalcone, TosMIC and methyl iodide under mild condition. All the synthesized compounds were screened for their bioactivity, and the MIC was determined, among which few compounds showed moderate antibacterial activity toward Gram-positive as well as Gram-negative bacteria. Further, cytotoxicity assay ascertained that these compounds were non-toxic to mammalian cells as well. The pyrene chromophore in the synthesized compounds (3a-e) and (5a-e) is responsible for the good photophysical properties which have an absorbance at λ 340 nm and emission at λ 410 nm. Hence, two of the selected novel synthesized compounds with non-cytotoxic nature prospected for bio-imaging of bacterial cells using high-content screening analysis show that the molecule is suitable for microbial imaging in pathological diagnostic studies.
authors.affiliation: Department of Organic Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
authors.forename: Mathiyazhagan
authors.initials: M
authors.lastname: Arun Divakar
authors.affiliation: Department of Organic Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
authors.forename: Sivakumar
authors.identifier: http://orcid.org/0000-0003-4087-7686
authors.initials: S
authors.lastname: Shanmugam
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000166:cytology* / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D061848:Optical Imaging; D011721:Pyrenes / Q000737:chemistry* / Q000494:pharmacology; D011758:Pyrroles / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D011721:Pyrenes; D011758:Pyrroles; C030984:pyrene
keywords: 
  IC
  50
  ; antibacterial; cell imaging; pyrene; pyrrole