title: Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells.
abstract: BACKGROUND
  Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP-13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis.
  
  
  METHODS
  Bone marrow-derived dendritic cells were obtained from C57BL/6 mice. One short-interfering RNA specific for MMP-13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry.
  
  
  RESULTS
  Compared to the control DCs, MMP-13-silenced DCs increased expression of anti-apoptosis-related genes, BAG1 (control group vs. MMP-13-silenced group: 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008), BCL-2 (control group vs. MMP-13-silenced group: 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036), and TP73 (control group vs. MMP-13-silenced group: 4.33 ± 0.29 vs. 5.60 ± 0.32, P = 0.001) and decreased apoptosis-related genes, CASP1 (control group vs. MMP-13-silenced group: 3.79 ± 0.67 vs. 2.54 ± 0.39, P = 0.019), LTBR (control group vs. MMP-13-silenced group: 9.23 ± 1.25 vs. 6.24 ± 1.15, P = 0.012), and CASP4 (control group vs. MMP-13-silenced group: 2.07 ± 0.56 vs. 0.35 ± 0.35, P = 0.002). Protein levels confirmed the same expression pattern. MMP-13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability.
  
  
  CONCLUSIONS
  These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
authors.affiliation: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Xin-Rui
authors.initials: XR
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Zhi-Hao
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Department of Dermatology Key Laboratory of Immunodermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Duo
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Dong
authors.affiliation: Department of Dermatology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Oncology, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, China.
authors.forename: Xian-Dong
authors.initials: XD
authors.lastname: Zeng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism* / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D034741:RNA, Small Interfering
chemical_list: D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D053509:Matrix Metalloproteinase 13