title: Tissue engineering with peripheral blood-derived mesenchymal stem cells promotes the regeneration of injured peripheral nerves.
abstract: Peripheral nerve injury repair can be enhanced by Schwann cell (SC) transplantation, but clinical applications are limited by the lack of a cell source. Thus, alternative systems for generating SCs are desired. Herein, we found the peripheral blood-derived mesenchymal stem cells (PBMSCs) could be induced into SC like cells with expressing SC-specific markers (S100, P75NTR and CNPase) and functional factors (NGF, NT-3, c-Fos, and Krox20). When the induced PBMSCs (iPBMSCs) were transplanted into crushed rat sciatic nerves, they functioned as SCs by wrapping the injured axons and expressing myelin specific marker of MBP. Furthermore, iPBMSCs seeded in an artificial nerve conduit to bridge a 10-mm defect in a sciatic nerve achieved significant nerve regeneration outcomes, including axonal regeneration and remyelination, nerve conduction recovery, and restoration of motor function, and attenuated myoatrophy and neuromuscular junction degeneration in the target muscle. Overall, the data from this study indicated that PBMSCs can transdifferentiate towards SC-like cells and have potential as grafting cells for nerve tissue engineering.
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Pan
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xianghai
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yijing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Guangdong Institutes of Biomedicine and Health, Chinese Academy Sciences, Guangzhou 510530, China.
authors.forename: Shangtao
authors.initials: S
authors.lastname: Cao
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jinkun
authors.initials: J
authors.lastname: Wen
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Qian
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhenqi
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Tan
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Joint Laboratory of Jinan University and The University of Hong Kong, GHM, Institute of CNS Regeneration, Jinan University, Guangzhou 510632, China; School of Biomedical Sciences, Li KaShing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China; State Key Laboratory of Brain and Cognitive Sciences, Li KaShing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
authors.forename: Wutian
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China; Key Laboratory of Tissue Construction and Detection of Guangdong Province, Guangzhou 510515, China; Institute of Bone Biology, Academy of Orthopedics, Guangdong Province, Guangzhou 510665, China. Electronic address: jiasongguo@aliyun.com.
authors.forename: Jiasong
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009186:Myelin Sheath / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D059348:Peripheral Nerve Injuries / Q000628:therapy*; D020127:Recovery of Function / Q000187:drug effects* / Q000502:physiology; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing
keywords: Differentiation; Nerve regeneration; Peripheral blood-derived mesenchymal cells; Peripheral nerve injury; Schwann cells; Tissue engineering