title: Amino-group carrier-protein-mediated secondary metabolite biosynthesis in Streptomyces.
abstract: Amino-group carrier proteins (AmCPs) mediate the biosynthesis of lysine and arginine in some bacteria and archaea. Here we demonstrate that an uncharacterized AmCP-mediated biosynthetic system functions to biosynthesize the previously uncharacterized and nonproteinogenic amino acid (2S,6R)-diamino-(5R,7)-dihydroxy-heptanoic acid (DADH) in Streptomyces sp. SANK 60404. DADH is incorporated into a novel peptide metabolite, vazabitide A, featuring an azabicyclo-ring structure, by nonribosomal peptide synthetases and successive modification enzymes in this bacterium. As the AmCP-mediated machinery for DADH biosynthesis is widely distributed in bacteria, further analysis of uncharacterized AmCP-containing gene clusters will lead to the discovery of novel bioactive compounds and novel biosynthetic enzymes.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Fumihito
authors.initials: F
authors.lastname: Hasebe
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuda
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Shiraishi
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Japan.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Futamura
authors.affiliation: Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Wako, Japan.|Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakano
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Tomita
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Ishigami
authors.affiliation: Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Taka
authors.affiliation: Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Mineki
authors.affiliation: Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fujimura
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Kuzuyama
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishiyama
mesh_terms: D001120:Arginine / Q000096:biosynthesis* / Q000737:chemistry; D002352:Carrier Proteins / Q000378:metabolism*; D008239:Lysine / Q000096:biosynthesis* / Q000737:chemistry; D064210:Secondary Metabolism; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D001120:Arginine; D008239:Lysine