title: Endometrial biopsy in Bos indicus beef heifers.
abstract: An endometrial biopsy allows for a comprehensive assessment of the uterine environment of a breeding female. Although routine in mares, devices used for endometrial biopsies are impracticable in heifers due to the size and structure of the cervix. This report describes the use of a human bronchoscopy biopsy device (Karl Storz®10366L) for collection of endometrial biopsies in Bos indicus beef heifers. The Storz®device is smaller and thinner and enabled the collection of an endometrial biopsy in 86% of heifers (n = 44/51). The biopsied tissue was of good quality and suitable for transcriptomic assessment of the endometrium, with total RNA yield and RNA integrity number (RIN) averaging 1.3 μg (range 0.4-5.3 μg) and 7.4 (range 5.7-8.4), respectively.
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton, Australia.
authors.forename: O
authors.identifier: http://orcid.org/0000-0003-4833-7094
authors.initials: O
authors.lastname: Ramirez-Garzon
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton, Australia.
authors.forename: N
authors.initials: N
authors.lastname: Satake
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton, Australia.
authors.forename: R E
authors.initials: RE
authors.lastname: Lyons
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Hill
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton, Australia.
authors.forename: M K
authors.initials: MK
authors.lastname: Holland
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton, Australia.
authors.forename: M
authors.initials: M
authors.lastname: McGowan
mesh_terms: D000818:Animals; D001706:Biopsy / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D004717:Endometrium / Q000502:physiology*; D005260:Female; D012313:RNA / Q000032:analysis; D020871:RNA Stability
chemical_list: D012313:RNA