title: New platform for simple and rapid protein-based affinity reactions.
abstract: We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.
authors.affiliation: Graduate School of Engineering, Kyoto University, Kyoto, Japan.|Analytical and Quality Evaluation Research Laboratories, Daiichi Sankyo Co., Ltd., Hiratsuka, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Kubota
authors.affiliation: Graduate School of Engineering, Kyoto University, Kyoto, Japan. kubo@anchem.mc.kyoto-u.ac.jp.
authors.forename: Takuya
authors.identifier: 0000-0002-9274-3295
authors.initials: T
authors.lastname: Kubo
authors.affiliation: Graduate School of Engineering, Kyoto University, Kyoto, Japan.|Chemco Scientific Co., Ltd., Osaka, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanigawa
authors.affiliation: Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Naito
authors.affiliation: Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Koji
authors.identifier: 0000-0003-1088-0569
authors.initials: K
authors.lastname: Otsuka
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000295:instrumentation*; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D008689:Methacrylates / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry / Q000378:metabolism; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D007074:Immunoglobulin G; D008689:Methacrylates; D011095:Polyethylenes; D011108:Polymers; D013205:Staphylococcal Protein A; C542536:poly(ethylene-co-glycidyl methacrylate); D010434:Pepsin A