title: Production of Odd-Carbon Dicarboxylic Acids in Escherichia coli Using an Engineered Biotin-Fatty Acid Biosynthetic Pathway.
abstract: Dicarboxylic acids are commodity chemicals used in the production of plastics, polyesters, nylons, fragrances, and medications. Bio-based routes to dicarboxylic acids are gaining attention due to environmental concerns about petroleum-based production of these compounds. Some industrial applications require dicarboxylic acids with specific carbon chain lengths, including odd-carbon species. Biosynthetic pathways involving cytochrome P450-catalyzed oxidation of fatty acids in yeast and bacteria have been reported, but these systems produce almost exclusively even-carbon species. Here we report a novel pathway to odd-carbon dicarboxylic acids directly from glucose in Escherichia coli by employing an engineered pathway combining enzymes from biotin and fatty acid synthesis. Optimization of the pathway will lead to industrial strains for the production of valuable odd-carbon diacids.
authors.affiliation: Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.|Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Haushalter
authors.affiliation: Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.|Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Phelan
authors.affiliation: Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.|Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Kristina M
authors.initials: KM
authors.lastname: Hoh
authors.affiliation: Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.|Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Cindy
authors.initials: C
authors.lastname: Su
authors.affiliation: Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.|Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: George
authors.initials: G
authors.lastname: Wang
authors.affiliation: Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.|Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Edward E K
authors.initials: EE
authors.lastname: Baidoo
authors.affiliation: Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.|Physical Bioscience Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Jay D
authors.identifier: 0000-0002-4910-2222
authors.initials: JD
authors.lastname: Keasling
mesh_terms: D053898:Biosynthetic Pathways; D001710:Biotin / Q000096:biosynthesis* / Q000737:chemistry; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D003998:Dicarboxylic Acids / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure; D015202:Protein Engineering
chemical_list: D003998:Dicarboxylic Acids; D005227:Fatty Acids; D001710:Biotin; D002244:Carbon