title: Geodermatophilus daqingensis sp. nov., isolated from petroleum-contaminated soil.
abstract: A novel Gram-positive actinobacterium, designated WT-2-1T, was isolated from a sample of petroleum-contaminated soil collected in Daqing, Heilongjiang province, China and characterised using a polyphasic taxonomic approach. The optimal growth for strain WT-2-1Twas found to be at 25-35 °C and at pH 6.0-9.0 and with 0-4% (w/v) NaCl, forming blackish green-coloured colonies. Chemotaxonomic and molecular characteristics of the isolate match those described for members of the genus Geodermatophilus. The peptidoglycan was found to contain meso-diaminopimelic acid; galactose, glucose and xylose were detected as diagnostic sugars. The main phospholipids were identified as diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and phosphatidylglycerol; MK-9(H4) was the dominant menaquinone present. The major cellular fatty acids were identified as iso-C16:0and iso-C15:0. 16S rRNA gene sequence analysis showed that strain WT-2-1Tis a member of the genus Geodermatophilus, with high sequence similarities to Geodermatophilus aquaeductus BMG801T(98.4%), Geodermatophilus saharensis CF5/5T(98.4%), Geodermatophilus bullaregiensis BMG841T(98.3%) and Geodermatophilus normandii CF5/3T(98.3%). Based on the phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, the isolate is concluded to represent a novel species of the genus Geodermatophilus, for which the name Geodermatophilus daqingensis sp. nov. is proposed. The type strain is WT-2-1T(=CGMCC 4.7381T= DSM 104001T).
authors.affiliation: College of Resources and Environment, Northeast Agricultural University Harbin, Harbin, 150030, People's Republic of China.|Daqing Normal University, Daqing, 163712, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People's Republic of China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Mathematics and Information Sciences of Guangxi University, Nanning, 530004, People's Republic of China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Mathematics, Jiamusi University, Jiamusi, 154007, People's Republic of China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Mathematics, Jiamusi University, Jiamusi, 154007, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People's Republic of China.
authors.forename: Yuanling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People's Republic of China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People's Republic of China.
authors.forename: Cui
authors.initials: C
authors.lastname: Huang
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Daqing Normal University, Daqing, 163712, People's Republic of China.
authors.forename: Guoling
authors.initials: G
authors.lastname: Ren
authors.affiliation: College of Resources and Environment, Northeast Agricultural University Harbin, Harbin, 150030, People's Republic of China. xuxiuhong99@yeah.net.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Xu
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial; D005227:Fatty Acids; D010578:Petroleum; D010743:Phospholipids; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010578:Petroleum; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: 16S rRNA gene; Geodermatophilus daqingensis sp.nov.; Petroleum-contaminated soil; Polyphasic taxonomy