title: Routine testing of Mycoplasma genitalium and Trichomonas vaginalis.
abstract: BACKGROUND
  Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) are common sexual transmitted infections (STI). However, most STI screening programmes do not include routinely detection of these pathogens. Consequently, epidemiological data about MG and TV in the general population is lacking. The current study aims to give insight into the prevalence of both infections, thereby guiding decisions whether testing for these pathogens should be included routinely.
  
  
  METHODS
  Between February 2013 and August 2015, all samples sent to the laboratory of Diakonessenhuis Utrecht for STI testing (i.e. testing for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG)) were additionally examined for the presence of MG and TV by means of a laboratory-developed RT-PCR. Samples were collected by our hospital or by regional general practitioners.
  
  
  RESULTS
  A total of 5628 PCR's were evaluated. In 7.5%, one or more STI were detected. CT was found in 5% and MG was positive in 1.9%. NG was detected in 0.5% and TV was detected in 0.6% of the samples. CT was found more often in primary care than in hospital setting (9.7% vs. 3.0%, p < .05). The same was shown for NG (1.1% vs. 0.2%, p < .05). More men than women were positive for CT (11.2% vs. 3.8%, p < .05) and NG (1.4% vs. 0.3%, p < .05).
  
  
  CONCLUSIONS
  MG is more prevalent than NG and TV in a regional Dutch population. Furthermore, TV is equally common as NG. Based on our prevalence data, including MG and TV in STI testing protocols should be considered in the future.
authors.affiliation: a Department of Obstetrics and Gynaecology , Diakonessenhuis , Utrecht , the Netherlands.
authors.forename: Marieke L M
authors.initials: ML
authors.lastname: Jenniskens
authors.affiliation: a Department of Obstetrics and Gynaecology , Diakonessenhuis , Utrecht , the Netherlands.
authors.forename: Jan H W
authors.initials: JH
authors.lastname: Veerbeek
authors.affiliation: a Department of Obstetrics and Gynaecology , Diakonessenhuis , Utrecht , the Netherlands.
authors.forename: Koen L
authors.initials: KL
authors.lastname: Deurloo
authors.affiliation: b Department of Medical Microbiology , St. Antonius Ziekenhuis, Nieuwegein , the Netherlands.
authors.forename: Erik J
authors.initials: EJ
authors.lastname: van Hannen
authors.affiliation: c Department of Medical Microbiology , Diakonessenhuis , Utrecht , the Netherlands.
authors.forename: Steven F T
authors.initials: SF
authors.lastname: Thijsen
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000208:ethnology; D045704:Mycoplasma genitalium / Q000235:genetics / Q000302:isolation & purification*; D009344:Neisseria gonorrhoeae; D009426:Netherlands / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012749:Sexually Transmitted Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D015231:Sexually Transmitted Diseases, Bacterial / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D014247:Trichomonas Vaginitis / Q000175:diagnosis / Q000453:epidemiology* / Q000208:ethnology; D014246:Trichomonas vaginalis / Q000235:genetics / Q000302:isolation & purification*
keywords: Mycoplasma genitalium; STI; Trichomonas vaginalis