title: CaSR and calpain contribute to the ischemia reperfusion injury of spinal cord.
abstract: Spinal cord ischemia reperfusion injury (SCIRI) can cause spinal cord dysfunction and even devastating paraplegia. Calcium-sensing receptor (CaSR) and calpain are two calcium related molecules which have been reported to be involved in the ischemia reperfusion injury of cardiomyocytes and the subsequent apoptosis. Here, we studied the expression of CaSR and calpain in spinal cord neurons and tissues, followed by the further investigation of the role of CaSR/calpain axis in the cellular apoptosis process during SCIRI. The results of in vitro and in vivo studies showed that the expression of CaSR and calpain in spinal cord neurons increased during SCIRI. Moreover, the CaSR agonist GdCl3and antagonist NPS-2390 enhanced or decreased the expression of CaSR and calpain respectively. The expressions of CaSR and calpain were also consistent with the cellular apoptosis in spinal cord. Taken together, CaSR-calpain contributes to the SCIRI apoptosis, and CaSR antagonist might be a helpful drug for alleviating SCIRI.
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215006, Jiangsu, China.
authors.forename: Ji-Fu
authors.initials: JF
authors.lastname: Sun
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou 215006, Jiangsu, China. Electronic address: 15358587399@163.com.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Yang
authors.affiliation: Department of Orthopedic Surgery, Affiliated Hospital of Jiangsu University, Jiangsu, China.
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Department of Histology and Embryology, Medical School of Jiangsu University, Jiangsu, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, Affiliated Hospital of Jiangsu University, Jiangsu, China.
authors.forename: Xing-Bing
authors.initials: XB
authors.lastname: Cao
authors.affiliation: Department of Orthopedic Surgery, Affiliated Hospital of Jiangsu University, Jiangsu, China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, Affiliated Hospital of Jiangsu University, Jiangsu, China.
authors.forename: Hao-Ming
authors.initials: HM
authors.lastname: Shu
authors.affiliation: Department of Orthopedic Surgery, Affiliated Hospital of Jiangsu University, Jiangsu, China.
authors.forename: Run-Yu
authors.initials: RY
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002154:Calpain / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D032383:Myocytes, Cardiac / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D044169:Receptors, Calcium-Sensing / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D020760:Spinal Cord Ischemia / Q000378:metabolism*
chemical_list: D044169:Receptors, Calcium-Sensing; D002154:Calpain
keywords: Apoptosis; Calcium-sensing receptor (CaSR); Calpain; Spinal cord ischemia reperfusion injury (SCIRI)