title: Structural analysis and enhanced production of fusaricidin from Paenibacillus kribbensis CU01 isolated from yellow loess.
abstract: A bacterial strain showing strong antifungal activity was isolated from yellow loess and was identified as Paenibacillus kribbensis CU01. Insoluble mucoidal polymers were separated from M9 culture medium via low-speed centrifugation. Most antifungal activity was associated with substances in the insoluble precipitate, which was purified by reverse phase high performance liquid chromatography. Purified fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Two major ion peaks with mass-to-charge ratio values (m/z) at 883.6 and 897.6 were revealed. After alkaline hydrolysis and sequence analysis, two cyclic depsipeptides were identified as, fusaricidin A and fusaricidin B. Their production was significantly increased by the addition of glucose, Fe2+, and Mn2+to M9 medium. Maximum concentrations of produced fusaricidin A and fusaricidin B at flask-scale comprised 460 mg L-1and 118 mg L-1, respectively: the highest production concentrations yet reported in the literature. This demonstrates that P. kribbensis CU01 has enormous commercial potential for the mass production of fusaricidin.
authors.affiliation: Department of Energy Convergence, Chosun University, Gwangju, Republic of Korea.
authors.forename: Jaewon
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Department of Energy Convergence, Chosun University, Gwangju, Republic of Korea.
authors.forename: Jong M
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Chul W
authors.initials: CW
authors.lastname: Lee
authors.affiliation: Department of Energy Convergence, Chosun University, Gwangju, Republic of Korea.|Department of Environmental Engineering, Chosun University, Gwangju, Republic of Korea.
authors.forename: Si W
authors.identifier: http://orcid.org/0000-0001-8539-6777
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology; D005947:Glucose / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology; D056507:Paenibacillus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D047630:Depsipeptides; D008670:Metals; C099200:fusaricidin A; C106436:fusaricidin B; D005947:Glucose
keywords: Paenibacillus kribbensis; antifungal substance; fusaricidin; glucose; metal ions; structural analysis