title: Developmental potential for tissue differentiation of fully dissociated cells of the ascidian embryo.
abstract: Initially, each tissue-progenitor blastomere of embryos of the ascidian Halocynthia was identified and isolated manually at the 110-cell (late-blastula) stage, the time at which most of the blastomeres have assumed a particular fate, such that each gives rise to a single type of tissue. The isolates were allowed to develop as partial embryos, then tissue differentiation was examined by monitoring the expression of specific molecular markers for differentiation of epidermis, endoderm, muscle and notochord. Essentially, all of the precursor blastomeres of these four kinds of tissue expressed the appropriate features of tissue differentiation in isolation, indicating that determination is already complete in most of the blastomeres by the 110-cell stage. Next, in order to evaluate the absolute capacity of cells for autonomous development, embryos were maintained continuously in a dissociated state from the first cleavage to the 110-cell stage, then the cells were allowed to develop into partial embryos. Tissue differentiation in the partial embryos was examined. The results showed the striking autonomy of the processes of segregation of developmental potential, as well as the autonomy of the processes of expression of differentiated phenotypes, namely those of epidermis and endoderm. Autonomous muscle differentiation was also observed; however, excess formation of "muscle" partial embryos occurred. The hypothesis that fate determination is mediated by localized maternal information in the egg cytoplasm is supported by the evidence of development of these tissues. By contrast, no evidence of notochord differentiation was observed in the partial embryos.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, Nagatsuda, Midori-ku, 227, Yokohama, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nishida
keywords: Ascidian embryos; Autonomous development; Blastomere dissociation; Blastomere isolation