title: The Effect of Chinese Herbal Medicine Formula mKG on Allergic Asthma by Regulating Lung and Plasma Metabolic Alternations.
abstract: Asthma is a chronic inflammatory disorder of the airway and is characterized by airway remodeling, hyperresponsiveness, and shortness of breath. Modified Kushen Gancao Formula (mKG), derived from traditional Chinese herbal medicines (TCM), has been demonstrated to have good therapeutic effects on experimental allergic asthma. However, its anti-asthma mechanism remains currently unknown. In the present work, metabolomics studies of biochemical changes in the lung tissue and plasma of ovalbumin (OVA)-induced allergic asthma mice with mKG treatment were performed using ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Partial least squares-discriminate analysis (PLS-DA) indicated that the metabolic perturbation induced by OVA was reduced after mKG treatment. A total of twenty-four metabolites involved in seven metabolic pathways were identified as potential biomarkers in the development of allergic asthma. Among them, myristic acid (L3orP2), sphinganine (L6orP4), and lysoPC(15:0) (L12orP16) were detected both in lung tissue and plasma. Additionally, l-acetylcarnitine (L1), thromboxane B2 (L2), 10-HDoHE (L10), and 5-HETE (L11) were first reported to be potential biomarkers associated with allergic asthma. The treatment of mKG mediated all of those potential biomarkers except lysoPC(15:0) (P16). The anti-asthma mechanism of mKG can be achieved through the comprehensive regulation of multiple perturbed biomarkers and metabolic pathways.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. yumeng.5555@163.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. 18911566112@126.com.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Jia
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. fengxiacui@163.com.
authors.forename: Feng-Xia
authors.initials: FX
authors.lastname: Cui
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. dsz200200@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. youngzhao91@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. mhyangmh@foxmail.com.
authors.forename: Mao-Hua
authors.initials: MH
authors.lastname: Yang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. zmzou@implad.ac.cn.
authors.forename: Zhong-Mei
authors.initials: ZM
authors.lastname: Zou
mesh_terms: D000108:Acetylcarnitine / Q000097:blood / Q000378:metabolism; D000818:Animals; D001249:Asthma / Q000209:etiology / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006967:Hypersensitivity / Q000150:complications / Q000378:metabolism*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D019814:Myristic Acid / Q000097:blood / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D013929:Thromboxane B2 / Q000097:blood / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D019814:Myristic Acid; D013929:Thromboxane B2; D000108:Acetylcarnitine; D013110:Sphingosine; C005682:safingol
keywords: Angelica oil; Glycyrrhiza uralensis; Sophora flavescens; UPLC-Q-TOF/MS; allergic asthma; biomarkers; mKG; metabolomics