title: Fission yeast tmsl protein abrogates normal development in Xenopus laevis embryos.
abstract: Recently we cloned tms1 (a putative dehydrogenase) by complementation of a human tumour-derived mutant p53 induced growth arrest in fission yeast. Microinjection of purified tmsl protein into Xenopus laevis embryos abrogated normal embryo development by causing cleavage retardation or cleavage arrest of injected blastomeres in a concentration dependant manner, whereas injection of specific affinity purified tms1 antiserum showed no significant morphological defects. Microinjection of tms1 protein together with affinity purified tms1 antibody resulted in a significantly reduced number of cleavage arrested embryos.
authors.affiliation: Medizinische Biochemie, Universität des Saarlandes, Gebdude 44, D-66421, Homburg/Saar, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Wagner
authors.affiliation: Abteilung Biochemie, Universität Ulm, Albert Einstein Allee 11, D-89069, Ulm, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hoever
authors.affiliation: Medizinische Biochemie, Universität des Saarlandes, Gebdude 44, D-66421, Homburg/Saar, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Appel
authors.affiliation: Abteilung Biochemie, Universität Ulm, Albert Einstein Allee 11, D-89069, Ulm, Germany.
authors.forename: Walter
authors.initials: W
authors.lastname: Knöchel
authors.affiliation: Medizinische Biochemie, Universität des Saarlandes, Gebdude 44, D-66421, Homburg/Saar, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Montenarh
keywords: Xenopus laevis development; fission yeast; tms1