title: Purity Evaluation of Curcuminoids in the Turmeric Extract Obtained by Accelerated Solvent Extraction.
abstract: Curcuminoids, the active principle of Curcuma longa L, is one of the most researched subjects worldwide for its broad-spectrum biological activities. Being traditionally known for their anticancer properties and issues related to bioavailability, the curcuminoids, including diferuloylmethane (curcumin), have gained special attention. Thus, the current study focused on the purity profiling of curcuminoids when extracted by accelerated solvent extraction, which was run with turmeric rhizome powder (20 g) at 1500 psi and at 50°C, with a static time of 10 min and with three cycles. The performance of ethanol, ethyl acetate, and acetone as extraction solvents was comparatively evaluated. Once extracted, the individual curcuminoids (curcumin, demethoxycurcumin, and bisdemethoxycurcumin) were purified by column chromatography, followed by preparative TLC, and the compounds were characterized by spectroscopic and chromatographic techniques. The HPLC method was standardized by using a gradient mobile phase of water and acetonitrile containing 0.1% formic acid. The LODs were calculated as 0.27, 0.33, and 0.42 μg/mL for curcumin, demethoxycurcumin, and bisdemethoxycurcumin, respectively. Accuracy (relative percentage error) and precision RSD values of the developed HPLC method were below 5%. The intraday accuracy ranged between -0.9 and -3.63%. The physical yield was the highest in ethanol (8.4%) extraction, followed by ethyl acetate (7.4%) and acetone (6.6%). Maximum purity was recorded in acetone (46.2%), followed by ethanol (43.4%) and ethyl acetate (38.8%), with no significant differences across the individual curcuminoids. This research will be useful for future applications related to the extraction of curcuminoids at a commercial level and to their profiling in food matrixes.
authors.affiliation: Indian Council of Agricultural Research-Indian Agricultural Research Institute, Division of Agricultural Chemicals, New Delhi 110 012, India.
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Yadav
authors.affiliation: Indian Council of Agricultural Research-Indian Agricultural Research Institute, Division of Agricultural Chemicals, New Delhi 110 012, India.
authors.forename: Khushbu
authors.initials: K
authors.lastname: Sharma
authors.affiliation: Indian Council of Agricultural Research-Indian Agricultural Research Institute, Division of Agricultural Chemicals, New Delhi 110 012, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Dutta
authors.affiliation: Indian Council of Agricultural Research-Indian Agricultural Research Institute, Division of Agricultural Chemicals, New Delhi 110 012, India.
authors.forename: Aditi
authors.initials: A
authors.lastname: Kundu
authors.affiliation: Indian Council of Agricultural Research-Indian Agricultural Research Institute, Division of Agricultural Chemicals, New Delhi 110 012, India.
authors.forename: Akanksha
authors.initials: A
authors.lastname: Awasthi
authors.affiliation: Indian Council of Agricultural Research-National Research Centre for Grapes, National Referral Laboratory, Pune 412 307, India.
authors.forename: Arnab
authors.initials: A
authors.lastname: Goon
authors.affiliation: Indian Council of Agricultural Research-National Research Centre for Grapes, National Referral Laboratory, Pune 412 307, India.
authors.forename: Kaushik
authors.initials: K
authors.lastname: Banerjee
authors.affiliation: Indian Council of Agricultural Research-Indian Agricultural Research Institute, Division of Agricultural Chemicals, New Delhi 110 012, India.
authors.forename: Supradip
authors.initials: S
authors.lastname: Saha
mesh_terms: D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012997:Solvents
chemical_list: D010936:Plant Extracts; D012997:Solvents; C012596:turmeric extract; D003474:Curcumin