title: Evaluation of Bone Marrow Processing Protocol for Therapeutic Applications via Culture and Characterization of Mesenchymal Stem Cells.
abstract: BACKGROUND
  Human mesenchymal stem cells from bone marrow (hMSCs) have broad therapeutic potential. These cells can be are readily isolated from bone marrow by their property to adhere to tissue culture treated culture wares. However, the proliferation rates and other properties of the cells gradually change during expansion. This study aims to validate the protocol of isolation and differentiation of hMSCs from bone marrow for therapeutic applications.
  
  
  METHODS
  Sixty ml of bone marrow was extracted from 5 patients and MSCs were isolated. These were characterized by Flow Cytometry, CFU assay and were differentiated into bone, fat cells and neurocytes.
  
  
  RESULTS
  The cells were having healthy morphology. These were positive for the markers CD105, CD90 and CD73 and negative for CD45, CD34 and HLA-DR. The cells could differentiate into fat, bone and neural cells.
  
  
  CONCLUSIONS
  MSCs from the bone marrow were isolated and differentiated. These cells were morphologically healthy and passed CFU assay. The cells exhibited differentiation potential into bone, fat and neural tissue. These cells can be used in therapeutic applications.
authors.affiliation: RegennMed Research and Therapeutics, Delhi, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Verma
authors.affiliation: RegennMed Research and Therapeutics, Delhi, India.|Mother Cell, Anupam Hospital, Rudrapur, Uttarakhand, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Bansal
authors.affiliation: RegennMed Research and Therapeutics, Delhi, India.|Mother Cell, Anupam Hospital, Rudrapur, Uttarakhand, India.
authors.forename: Anupama
authors.initials: A
authors.lastname: Agrawal
authors.affiliation: Advanced Health Institute, Puerto Rico, USA.
authors.forename: Jerry
authors.initials: J
authors.lastname: Leon
authors.affiliation: Department of Anthropology, Wayne State University, Detroit, Michigan, USA.
authors.forename: I Birgitta
authors.initials: IB
authors.lastname: Sundell
authors.affiliation: Haffkine Institute, Mumbai, India.
authors.forename: Prasad S
authors.initials: PS
authors.lastname: Koka
mesh_terms: D050156:Adipogenesis; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D048430:Cell Shape; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D013194:Staining and Labeling
chemical_list: D015415:Biomarkers