title: In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform.
abstract: Nanomaterials without chemical linkers or physical interactions that reside on a two-dimensional surface are attractive because of their electronic, optical and catalytic properties. An in situ method has been developed to fabricate gold nanoparticle (Au NP) films on different substrates, regardless of whether they are hydrophilic or hydrophobic surfaces, including glass, 96-well polystyrene plates, and polydimethylsiloxane (PDMS). A mixture of sodium formate (HCOONa) and chloroauric acid (HAuCl4) solution was used to prepare Au NP films at room temperature. An experimental study of the mechanism revealed that film formation is dependent on surface wettability and inter particle attraction. The as-fabricated Au NP films were further applied to the colorimetric detection of influenza virus. The response to the commercial target, New Caledonia/H1N1/1999 influenza virus, was linear in the range from 10 pg/ml to 10 μg/ml and limit of detection was 50.5 pg/ml. In the presence of clinically isolated influenza A virus (H3N2), the optical density of developed color was dependent on the virus concentration (10-50,000 PFU/ml). The limit of detection of this study was 24.3 PFU/ml, a limit 116 times lower than that of conventional ELISA (2824.3 PFU/ml). The sensitivity was also 500 times greater than that of commercial immunochromatography kits.
authors.affiliation: Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Syed Rahin
authors.initials: SR
authors.lastname: Ahmed
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Korea.
authors.forename: Jeonghyo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Korea.
authors.forename: Van Tan
authors.initials: VT
authors.lastname: Tran
authors.affiliation: Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Ontario, Canada.
authors.forename: Suresh
authors.initials: S
authors.lastname: Neethirajan
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Korea.
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
authors.affiliation: Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.|Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
mesh_terms: D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification* / Q000472:pathogenicity; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification* / Q000472:pathogenicity; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D053768:Metal Nanoparticles / Q000737:chemistry
chemical_list: D006046:Gold