title: Aggregative properties of different cell types of the fresh-water spongeEphydatia fluviatilis isolated on ficoll gradients.
abstract: Cell suspensions of the fresh-watersponge Ephydatia fluviatilis have been fractionated by means ofFicoll gradient centrifugation. Three fractions were isolated. The densest contains archeocyte-like cells only; the intermediate fraction is very rich in choanocytes, and the lightest is a mixture of cell types. Earch fraction shows specificaggregative properties and potentialities to reconstitute functional sponges.It appears that the sequence of reconstitution events can be selectively altered by certain disequilibria in the cell populationThese preliminary results constitute a first approach to the analysis ofcell type specificity in sponges.
authors.affiliation: Laboratoire de Biologie animale et cellulaire, Université libre de Bruxelles, 50, av. F.D. Roosevelt, 1050, Bruxelles, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: De Sutter
authors.affiliation: Laboratoire de Biologie animale et cellulaire, Université libre de Bruxelles, 50, av. F.D. Roosevelt, 1050, Bruxelles, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Van de Vyver
keywords: Aggregative Properties; Cell type specificity; Ficoll gradient; Sponge