title: The GAGA factor regulatory network: Identification of GAGA factor associated proteins.
abstract: The Drosophila GAGA factor (GAF) has an extraordinarily diverse set of functions that include the activation and silencing of gene expression, nucleosome organization and remodeling, higher order chromosome architecture and mitosis. One hypothesis that could account for these diverse activities is that GAF is able to interact with partners that have specific and dedicated functions. To test this possibility we used affinity purification coupled with high throughput mass spectrometry to identify GAF associated partners. Consistent with this hypothesis the GAF interacting network includes a large collection of factors and complexes that have been implicated in many different aspects of gene activity, chromosome structure and function. Moreover, we show that GAF interactions with a small subset of partners is direct; however for many others the interactions could be indirect, and depend upon intermediates that serve to diversify the functional capabilities of the GAF protein.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Lomaev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Mikhailova
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Maksim
authors.initials: M
authors.lastname: Erokhin
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Shaposhnikov
authors.affiliation: Department of Chemical Physiology, SR302B, The Scripps Research Institute, La Jolla, California, United States of America.
authors.forename: James J
authors.initials: JJ
authors.lastname: Moresco
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Blokhina
authors.affiliation: Department of Molecular Biology Princeton University, Princeton, NJ, United States of America.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wolle
authors.affiliation: Department of Molecular Biology Princeton University, Princeton, NJ, United States of America.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Aoki
authors.affiliation: Institute of Animal Physiology, Biochemistry and Nutrition, Borovsk, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Ryabykh
authors.affiliation: Department of Chemical Physiology, SR302B, The Scripps Research Institute, La Jolla, California, United States of America.
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Yulii V
authors.initials: YV
authors.lastname: Shidlovskii
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Georgiev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.|Department of Molecular Biology Princeton University, Princeton, NJ, United States of America.
authors.forename: Paul
authors.initials: P
authors.lastname: Schedl
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Darya
authors.identifier: http://orcid.org/0000-0003-0008-3814
authors.initials: D
authors.lastname: Chetverina
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000502:physiology*; D024202:Electrophoretic Mobility Shift Assay; D013058:Mass Spectrometry; D011817:Rabbits; D014157:Transcription Factors / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C063460:Trl protein, Drosophila; D000255:Adenosine Triphosphate