title: Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System.
abstract: 1-Aminocyclopropane-1-carboxylic acid (ACC) is a biosynthetic precursor of ethylene. The movement of ACC across the plasma membrane (PM) has been implicated in various physiological contexts during environmental adaptation and differentiation in higher plants. A PM-localized transporter in Arabidopsis thaliana, LYSINE HISTIDINE TRANSPORTER1 (LHT1) participates in the uptake of ACC, implicating a class of amino-acid transporters in the transport of ACC. Here, we describe the method for assaying uptake of ACC into the plant cells using an Arabidopsis mesophyll protoplast system.
authors.affiliation: Department of Life Science, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Song
authors.affiliation: Department of Integrative Bioscience and Biotechnology, College of Life Science, Sejong University, Seoul, Republic of Korea.
authors.forename: Sumin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Integrative Bioscience and Biotechnology, College of Life Science, Sejong University, Seoul, Republic of Korea. soh@sejong.ac.kr.
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Soh
mesh_terms: D000598:Amino Acids, Cyclic / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D002458:Cell Fractionation / Q000379:methods; D018515:Plant Leaves; D011523:Protoplasts / Q000378:metabolism*
chemical_list: D000598:Amino Acids, Cyclic; C023863:1-aminocyclopropane-1-carboxylic acid
keywords: ACC uptake; Amino acid transporter; Arabidopsis; Plasma membrane; Protoplast