title: Dietary Conjugated Linoleic Acid-c9t11 Prevents Collagen-Induced Arthritis, Whereas Conjugated Linoleic Acid-t10c12 Increases Arthritic Severity.
abstract: Two conjugated linoleic acid (CLA) isomers, cis-9, trans-11 (CLAc9t11) and trans-10, cis-12 (CLAt10c12), reduce inflammation in a number of animal models, including collagen-induced arthritis (CA). However, little is known about the ability of individual CLA isomers to prevent autoimmune disease onset. Evidence that mixed isomer CLA drives T helper cell (Th) 1 responses suggests that CLA, or a specific isomer, exacerbates onset of Th1 autoimmune diseases. In two experiments, we examined if prior dietary exposure to CLAt10c12 (experiment 1) or CLAc9t11 (experiment 2) affected the incidence or severity of CA. DBA/1 mice were fed a semi purified diet with either 6% corn oil (CO, w/w), 5.75% CO plus 0.25% CLAt10c12, or 5.5% CO plus 0.5% CLAc9t11 prior to arthritis development. Arthritis incidence and severity, anti-collagen antibodies, paw cytokines, and hepatic fatty acids were measured. CLAt10c12 had no effect on arthritis incidence but increased arthritic severity (42%, P = 0.02); however, CLAc9t11 decreased arthritis incidence 39% compared to CO fed mice (P = 0.01), but had no effect on disease severity. CLAt10c12-induced increase in anti-collagen type II IgG antibodies may be a mechanism by which this isomer increased arthritic severity, and CLAc9t11-induced increase in Th2 paw cytokines (IL-4 and IL-10, P ≤ 0.04) may explain how CLAc9t11 reduced the arthritis incidence. While both isomers are well known to reduce inflammation in arthritic mice, these new data suggest isomer differences when fed prior to autoimmune disease.
authors.affiliation: Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, Madison, WI, 53706, USA.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Muhlenbeck
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Butz
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA.
authors.forename: Jake M
authors.initials: JM
authors.lastname: Olson
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA.
authors.forename: Daniela
authors.initials: D
authors.lastname: Uribe-Cano
authors.affiliation: Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, Madison, WI, 53706, USA. mcook@wisc.edu.|Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI, 53706, USA. mcook@wisc.edu.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Cook
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000453:epidemiology* / Q000276:immunology / Q000517:prevention & control; D003314:Corn Oil / Q000008:administration & dosage* / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology; D004359:Drug Therapy, Combination; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage* / Q000494:pharmacology; D051379:Mice; D008811:Mice, Inbred DBA; D011897:Random Allocation; D012720:Severity of Illness Index; D016896:Treatment Outcome
chemical_list: D016207:Cytokines; D004041:Dietary Fats; D044243:Linoleic Acids, Conjugated; C496197:trans-10,cis-12-conjugated linoleic acid; C046938:9,11-linoleic acid; D003314:Corn Oil
keywords: Anti-collagen antibodies; Arthritis; Conjugated linoleic acid; Th1 cytokines; Th1/Th2; Th2 cytokines