title: miR319, miR390, and miR393 Are Involved in Aluminum Response in Flax (Linum usitatissimumL.).
abstract: Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax (Linum usitatissimumL.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl3solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified. miR319, miR390, and miR393 revealed expression alterations associated with Al treatment of flax plants. Moreover, for miR390 and miR393, the alterations were distinct in sensitive and resistant to Al genotypes. Expression level changes of miR319 and miR390 were confirmed using qPCR analysis. In flax, potential targets of miR319 are TCPs, miR390-TAS3 and GRF5, and miR393-AFB2-coding transcripts. TCPs, TAS3, GRF5, and AFB2 participate in regulation of plant growth and development. The involvement of miR319, miR390, and miR393 in response to Al stress in flax was shown here for the first time. We speculate that these microRNAs play an important role in Al responseviaregulation of growth processes in flax plants.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Alexey A
authors.identifier: 0000-0002-6827-9584
authors.initials: AA
authors.lastname: Dmitriev
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Anna V
authors.identifier: 0000-0002-3722-8207
authors.initials: AV
authors.lastname: Kudryavtseva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Nadezhda L
authors.identifier: 0000-0002-8379-623X
authors.initials: NL
authors.lastname: Bolsheva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Zyablitsin
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia; All-Russian Research Institute for Flax, Torzhok 172002, Russia.
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Rozhmina
authors.affiliation: All-Russian Research Institute for Flax, Torzhok 172002, Russia.
authors.forename: Natalya V
authors.identifier: 0000-0003-4454-6948
authors.initials: NV
authors.lastname: Kishlyan
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: George S
authors.identifier: 0000-0002-6493-8378
authors.initials: GS
authors.lastname: Krasnov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Speranskaya
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Anastasia A
authors.identifier: 0000-0002-0653-3655
authors.initials: AA
authors.lastname: Krinitsina
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Asiya F
authors.initials: AF
authors.lastname: Sadritdinova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Anastasiya V
authors.identifier: 0000-0002-4421-4364
authors.initials: AV
authors.lastname: Snezhkina
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Maria S
authors.identifier: 0000-0002-6893-4673
authors.initials: MS
authors.lastname: Fedorova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Olga Yu
authors.identifier: 0000-0003-4124-2367
authors.initials: OY
authors.lastname: Yurkevich
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Olga V
authors.identifier: 0000-0003-3150-8598
authors.initials: OV
authors.lastname: Muravenko
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Maxim S
authors.identifier: 0000-0002-6556-163X
authors.initials: MS
authors.lastname: Belenikin
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Nataliya V
authors.identifier: 0000-0001-8083-3018
authors.initials: NV
authors.lastname: Melnikova
mesh_terms: D000535:Aluminum / Q000737:chemistry*; D018556:Crops, Agricultural / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019597:Flax / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D005838:Genotype; D035683:MicroRNAs / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000378:metabolism*; D015398:Signal Transduction; D012987:Soil / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D012987:Soil; D000535:Aluminum