title: The function of type IV collagen during Drosophila embryogenesis.
abstract: A collagen gene (Dcg1) was characterized in Drosophila melanogaster and shown to encode a peptide related to vertebrate basement membrane type IV collagen chains. To study the function of type IV collagen during Drosophila development, we transformed flies with a partially truncated Dcg1 gene under the control of a heat-shock promotor. This construct induced synthesis of shortened pro-α chains which associated with normal ones and thereby caused degradation of the shortened and normal pro-α chains through a process called "pro-collagen suicide". A large proportion of embryos expressing the transgene developed a phenotype exhibiting absence or partial retraction of the germ band with defects in nerve cord condensation and dorsal closure. Together these results indicated that, during embryogenesis, type IV collagen was an essential guiding factor for cell-matrix interactions in morphogenetic events.
authors.affiliation: Institut de Biologie du Développement de Marseille, CNRS-INSERM-Université Aix-Marseille II, Laboratoire de Génétique et Physiologic du Développement, UMCR 9943, F-13288, Faculté des Sciences de Luminy, Marseille Cedex 9, France.
authors.forename: C
authors.initials: C
authors.lastname: Borchiellini
authors.affiliation: Institut de Biologie du Développement de Marseille, CNRS-INSERM-Université Aix-Marseille II, Laboratoire de Génétique et Physiologic du Développement, UMCR 9943, F-13288, Faculté des Sciences de Luminy, Marseille Cedex 9, France.
authors.forename: J
authors.initials: J
authors.lastname: Coulon
authors.affiliation: Institut de Biologie du Développement de Marseille, CNRS-INSERM-Université Aix-Marseille II, Laboratoire de Génétique et Physiologic du Développement, UMCR 9943, F-13288, Faculté des Sciences de Luminy, Marseille Cedex 9, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Le Parco
keywords: Dorsal closure; Drosophila; Germ band retraction; Nerve cord condensation; Type IV collagen