title: Incorporation of calliphorin into the cuticle of the developing blowfly, Calliphora vicina.
abstract: The stage-specific appearance of calliphorin in cuticles of Calliphora vicina was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The fate of the protein, injected into last instar larvae, was pursued by autoradiography of histological sections. Fractionation of sclerotized pupal cuticle in buffer-soluble, urea-soluble and NaOH-soluble fractions shows that calliphorin forms covalent and non-covalent links with other cuticle components. Calliphorin traverses the epidermal cells and enters the cuticle in an undegraded state and appears to be an important constituent of the sclerotizing system.
authors.affiliation: Zoologisches Institut der Universität, Röntgenring 10, D-8700, Würzburg, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: König
authors.affiliation: Zoologisches Institut der Universität, Röntgenring 10, D-8700, Würzburg, Germany.
authors.forename: Om P
authors.initials: OP
authors.lastname: Agrawal
authors.affiliation: Zoologisches Institut der Universität, Röntgenring 10, D-8700, Würzburg, Germany.
authors.forename: Heide
authors.initials: H
authors.lastname: Schenkel
authors.affiliation: Zoologisches Institut der Universität, Röntgenring 10, D-8700, Würzburg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Scheller
keywords: Calliphora vicina; Calliphorin; Cuticle; Larval serum proteins (arylphorin); Sclerotization