title: Characterization of the Physicochemical Properties of β-Cyclodextrin-Divinyl Sulfone Polymer Carrier-Bile Acid Systems.
abstract: Herein, we report on the systematic design and characterization of cross-linked polymer carriers containing β-cyclodextrin (β-CD) and divinyl sulfone (DVS). The polymer carriers were prepared at variable feed ratios (β-CD-DVS; 1:1, 1:2, 1:3, and 1:6) and characterized using spectroscopy (IR,1H solution NMR, and13C CP-MAS solids NMR spectroscopy), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and a dye decolorization method using phenolphthalein. Uptake studies were carried out at pH 9.00 for the polymer carriers using single component bile acids (cholic acid, deoxycholic acid, glycodeoxycholic acid, and taurodeoxycholic acid). Equilibrium uptake results were evaluated by the Langmuir isotherm model where variable equilibrium parameters were related to the relative apolar character of the bile acid. The Langmuir model yields a carrier/bile acid binding affinity of ∼103M-1where the lipophilic inclusion sites of the polymer play a prominent role, while the DVS linker framework sites have a lower adsorption affinity, in accordance with the greater hydrophilic character of such sites.
authors.affiliation: Department of Chemistry, University of Saskatchewan , 110 Science Place, Saskatoon, Saskatchewan S7N 5C9, Canada.
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Mohamed
authors.affiliation: Department of Chemistry, University of Saskatchewan , 110 Science Place, Saskatoon, Saskatchewan S7N 5C9, Canada.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Water Science and Technology Directorate, Environment and Climate Change Canada , 11 Innovation Boulevard, Saskatoon, Saskatchewan S7N 3H5, Canada.
authors.forename: Kerry M
authors.initials: KM
authors.lastname: Peru
authors.affiliation: Water Science and Technology Directorate, Environment and Climate Change Canada , 11 Innovation Boulevard, Saskatoon, Saskatchewan S7N 3H5, Canada.
authors.forename: John V
authors.initials: JV
authors.lastname: Headley
authors.affiliation: Department of Chemistry, University of Saskatchewan , 110 Science Place, Saskatoon, Saskatchewan S7N 5C9, Canada.
authors.forename: Lee D
authors.identifier: 0000-0002-0688-3102
authors.initials: LD
authors.lastname: Wilson
mesh_terms: D000086:Acetazolamide / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D050091:Dendrimers / Q000737:chemistry; D005909:Glioblastoma / Q000378:metabolism; D006801:Humans; D008823:Micelles; D011108:Polymers / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D018874:Spheroids, Cellular / Q000187:drug effects; D000077204:Temozolomide; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D008823:Micelles; D011108:Polymers; D047392:beta-Cyclodextrins; D003606:Dacarbazine; D011794:Quercetin; D000086:Acetazolamide; D000077204:Temozolomide
keywords: adsorption; bile acids; cyclodextrin; divinyl sulfone; isotherm