title: Live birth after replacement of an embryo obtained from a spontaneously in vitro matured metaphase-I oocyte.
abstract: This case report describes a live birth after the fresh replacement of an embryo obtained from a spontaneously in vitro matured oocyte. The patient was subjected to controlled ovarian stimulation for IVF treatment, obtaining two oocytes. One was found to be immature at the time of denudation, at metaphase-I. This immature oocyte was kept in culture overnight in standard conditions along with the second oocyte - which was mature but failed to fertilize - spontaneously achieving metaphase-II, and was subjected to ICSI. The resulting embryo was replaced on the second day of development, producing a pregnancy that resulted in a healthy live birth. Post-denudation in vitro maturation could be considered as a tool to improve reproductive outcomes in selected patients, such as poor responders.
authors.affiliation: a Clínicas Ginemed Sevilla , Seville , Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Piqueras
authors.affiliation: b Clínicas Ginemed , Lisboa , Lisbon , Portugal.
authors.forename: Miguel
authors.initials: M
authors.lastname: Gallardo
authors.affiliation: a Clínicas Ginemed Sevilla , Seville , Spain.
authors.forename: María
authors.initials: M
authors.lastname: Hebles
authors.affiliation: a Clínicas Ginemed Sevilla , Seville , Spain.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Jiménez
authors.affiliation: a Clínicas Ginemed Sevilla , Seville , Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Migueles
authors.affiliation: b Clínicas Ginemed , Lisboa , Lisbon , Portugal.
authors.forename: Lorena
authors.initials: L
authors.lastname: Montero
authors.affiliation: a Clínicas Ginemed Sevilla , Seville , Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Sánchez-Martín
authors.affiliation: a Clínicas Ginemed Sevilla , Seville , Spain.
authors.forename: Pascual
authors.initials: P
authors.lastname: Sánchez-Martín
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D050498:Live Birth; D008297:Male; D011247:Pregnancy
keywords: Human oocyte; immature; in vitro maturation; post-denudation; spontaneous