title: Development of Novel Piezoelectric Biosensor Using PZT Ceramic Resonator for Detection of Cancer Markers.
abstract: A novel biosensor based on piezoelectric ceramic resonator was developed for direct detection of cancer markers in the study. For the first time, a commercially available PZT ceramic resonator with high resonance frequency was utilized as transducer for a piezoelectric biosensor. A dual ceramic resonators scheme was designed wherein two ceramic resonators were connected in parallel: one resonator was used as the sensing unit and the other as the control unit. This arrangement minimizes environmental influences including temperature fluctuation, while achieving the required frequency stability for biosensing applications. The detection of the cancer markers Prostate Specific Antigen (PSA) and α-Fetoprotein (AFP) was carried out through frequency change measurement. The device showed high sensitivity (0.25 ng/ml) and fast detection (within 30 min) with small samples (1 μl), which is compatible with the requirements of clinical measurements. The results also showed that the ceramic resonator-based piezoelectric biosensor platform could be utilized with different chemical interfaces, and had the potential to be further developed into biosensor arrays with different specificities for simultaneous detection of multiple analytes.
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.|Key Laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen, 518057, China.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.|Key Laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen, 518057, China.
authors.forename: Chi-Chun
authors.initials: CC
authors.lastname: Fong
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.|Key Laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen, 518057, China.
authors.forename: Pik-Yuan
authors.initials: PY
authors.lastname: Cheung
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. bhmyang@cityu.edu.hk.|Key Laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen, 518057, China. bhmyang@cityu.edu.hk.
authors.forename: Mengsu
authors.initials: M
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002516:Ceramics / Q000737:chemistry*; D004867:Equipment Design; D019220:High-Energy Shock Waves; D006801:Humans; D007854:Lead / Q000737:chemistry*; D008962:Models, Theoretical; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; C065536:lead titanate zirconate; D007854:Lead; D015040:Zirconium; D014025:Titanium
keywords: Ceramic resonator; Piezoelectric; Prostate specific antigen; α-fetoprotein