title: Evaluation of antioxidant and anticancer activity of copper oxide nanoparticles synthesized using medicinally important plant extracts.
abstract: Copper oxide (CuO) nanoparticles were synthesized by green chemistry approach using different plant extracts obtained from the leaves of Azadirachta indica, Hibiscus rosa-sinensis, Murraya koenigii, Moringa oleifera and Tamarindus indica. In order to compare their efficiency, the same copper oxide nanoparticles was also synthesized by chemical method. Phytochemical screening of the leaf extracts showed the presence of carbohydrates, flavonoids, glycosides, phenolic compounds, saponins, tannins, proteins and amino acids. FT IR spectra confirmed the possible biomolecules responsible for the formation of copper oxide nanoparticles. The surface plasmon resonance absorption band at 220-235nm in the UV-vis spectra also supports the formation of copper oxide nanoparticles. XRD patterns revealed the monoclinic phase of the synthesized copper oxide nanoparticles. The average size, shape and the crystalline nature of the nanoparticles were determined by SEM, TEM and SAED analysis. EDX analysis confirmed the presence of elements in the synthesized nanoparticles. The antioxidant activity was evaluated by three different free radical scavenging assays. The cytotoxicity of copper oxide nanoparticles was evaluated against four cancer cell lines such as human breast (MCF-7), cervical (HeLa), epithelioma (Hep-2) and lung (A549), and one normal human dermal fibroblast (NHDF) cell line. The morphological changes were evaluated using Hoechst 33258 staining assay. Copper oxide nanoparticles synthesized by green method exhibited high antioxidant and cytotoxicity than that synthesized by chemical method.
authors.affiliation: Post-Graduate and Research Department of Chemistry, The New College (Autonomous), Chennai 600 014, India; Department of Chemistry, Justice Basheer Ahmed Sayeed College for Women (Autonomous), Chennai 600 018, India.
authors.forename: Dilaveez
authors.initials: D
authors.lastname: Rehana
authors.affiliation: Post-Graduate and Research Department of Chemistry, The New College (Autonomous), Chennai 600 014, India.
authors.forename: D
authors.initials: D
authors.lastname: Mahendiran
authors.affiliation: Department of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy, Tiruchengodu 637 205, India.
authors.forename: R Senthil
authors.initials: RS
authors.lastname: Kumar
authors.affiliation: Post-Graduate and Research Department of Chemistry, The New College (Autonomous), Chennai 600 014, India. Electronic address: akrahmanjkr@gmail.com.
authors.forename: A Kalilur
authors.initials: AK
authors.lastname: Rahiman
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D000975:Antioxidants / Q000138:chemical synthesis / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D064209:Phytochemicals; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D003300:Copper; C030973:cupric oxide
keywords: Antioxidant activity; Apoptosis; CuO nanoparticles; Green synthesis; MTT assay