title: DNA-mediated inhibition of peroxidase-like activities on platinum nanoparticles for simple and rapid colorimetric detection of nucleic acids.
abstract: In this research, we found that the peroxidase-like activities of noncovalent DNA-Pt hybrid nanoparticles could be obviously blocked, when Pt nanoparticles (PtNPs) were synthesized in situ using DNA as a template. Moreover, this self-assembled synthetic process was very convenient and rapid (within few mintues), and the inhibition mediated by DNA was also very effective. First, by the paper-based analytical device (PAD) we found the catalytic activities of DNA-Pt hybrid nanoparticles exhibited a linear response to the concentration of DNA in the range from 0.0075 to 0.25µM. Then, with the magnetic bead isolated system and target DNA-induced hybridization chain reaction (HCR), we realized the specific target DNA analysis with a low detection of 0.228nM, and demonstrated its effectivity in distinguishing the target DNA from other interferences. To our knowledge, this is the first report that used the nanoassembly between DNA and PtNPs for colorimetric detection of nucleic acids, which was based on DNA-mediated inhibition of catalytic activities of platinum nanoparticles. The results may be useful for understanding the interactions between DNA and metal nanoparticles, and for development of other convenient and effective analytical strategies.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China. Electronic address: fxech@fudan.edu.cn.
authors.forename: Xueen
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China. Electronic address: jlkong@fudan.edu.cn.
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry; D010984:Platinum / Q000737:chemistry
chemical_list: D009696:Nucleic Acids; D010984:Platinum; D006046:Gold; D004247:DNA; D009195:Peroxidase
keywords: DNA-Pt hybrid nanoparticles; Noncovalent assemble; Paper-based analytical device; Peroxidase-like activities