title: All-Electrical Graphene DNA Sensor Array.
abstract: Electrical sensing of biomolecules has been an important pursuit due to the label-free operation and chip-scale construct such sensing modality can enable. In particular, electrical biomolecular sensors based on nanomaterials such as semiconductor nanowires, carbon nanotubes, and graphene have demonstrated high sensitivity, which in the case of nanowires and carbon nanotubes can surpass typical optical detection sensitivity. Among these nanomaterials, graphene is well suited for a practical candidate for implementing a large-scale array of biomolecular sensors, as its two-dimensional morphology is readily compatible with industry standard top-down fabrication techniques. In our recent work published in 2014 Nature Communications, we demonstrated these benefits by creating DNA sensor arrays from chemical vapor deposition (CVD) graphene. The present chapter, which is a review of this recent work, outlines procedures demonstrating the use of individual graphene sites of the array in dual roles--electrophoretic electrodes for site specific probe DNA immobilization and field effect transistor (FET) sensors for detection of target DNA hybridization. The 100 fM detection sensitivity achieved in 7 out of 8 graphene FET sensors in the array combined with the alternative use of the graphene channels as electrophoretic electrodes for probe deposition represent steps toward creating an all-electrical multiplexed DNA array.
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Abbott
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
authors.forename: Donhee
authors.initials: D
authors.lastname: Ham
authors.affiliation: Department of Electrical and Computer Engineering, University of Massachusetts, 100 Natural Resources Road, Amherst, MA, 01003, USA. guangyux@umass.edu.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry; D015342:DNA Probes; D004560:Electricity; D004581:Electronics / Q000295:instrumentation / Q000379:methods*; D004586:Electrophoresis / Q000379:methods; D006108:Graphite / Q000737:chemistry; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D061848:Optical Imaging / Q000379:methods
chemical_list: D015342:DNA Probes; D006108:Graphite; D004247:DNA
keywords: Bioelectronics; Biomolecule sensing; DNA detection; DNA sensor array; Electrophoresis; Graphene