title: OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments.
abstract: Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method. The method takes advantage of both homologous recombination enzymes in E. coli and overlap PCR. Using OEPR cloning, a long fragment (1-6 kb) or multiple fragments (2-4 fragments) can be easily constructed and simultaneously assembled into a target vector.
authors.affiliation: Research Center of Biological Information, College of Science, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Liu
authors.affiliation: Beijing Institute of Pharmaceutical Chemistry, Beijing 102205, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Research Center of Biological Information, College of Science, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Research Center of Biological Information, College of Science, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Zhu
authors.affiliation: Research Center of Biological Information, College of Science, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Er
authors.initials: E
authors.lastname: Meng
authors.affiliation: Research Center of Biological Information, College of Science, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Dong-Yi
authors.initials: DY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D059765:Homologous Recombination / Q000235:genetics*; D006801:Humans; D016254:Mutagenesis, Insertional / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats
chemical_list: D004247:DNA