title: Cytotoxic Effect of Thymus caramanicus Jalas on Human Oral Epidermoid Carcinoma KB Cells.
abstract: Identifying new chemotherapeutic agents with fewer side effects is a major concern for scientists today. Thymus caramanicus Jalas (Lamiaceae family) is one of the species of Thymus that grows wild in different regions of Iran. Traditionally, leaves of this plant are used in the treatment of diabetes, arthritis and cancer. Here was investigated the cytotoxic property of Thymus caramanicus essential oil and extract in human oral epidermoid carcinoma KB cells. Cell viability was measured by MTT and neutral red assays. The cells were exposed to different concentrations of essential oil (0.05-1 µL/mL) and extract (25-150 µg/mL) for 24 h. Doxorubicin was used as anticancer control drug. The data showed that the essential oil (IC50=0.44 µL/mL) and extract (IC50=105 µg/mL) induce potent cytotoxic property. Surprisingly, cytotoxic effects of essential oil and extract of this plant on KB cancer cells were greater than those on normal gingival HGF1-PI1 cell line. In addition, Thymus caramanicus could potentiate the effect of doxorubicin in sub-effective concentrations. The results of the present study indicate that essential oils and extracts of Thymus caramanicus have potential anti-proliferative property on KB cells and can be used as pharmaceutical case study for oral cancer treatments.
authors.affiliation: Department of Periodontology, Laser Research Center in Medical Sciences (LRCMS), AJA University of Medical Sciences, Tehran, Iran.|International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Fekrazad
authors.affiliation: Persian Gulf Oral and Dental Disease Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.|Laser research center in medical Sciences, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Mehrad
authors.initials: M
authors.lastname: Afzali
authors.affiliation: Exercise Physiology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.
authors.forename: Hamzeh
authors.initials: H
authors.lastname: Pasban-Aliabadi
authors.affiliation: Laboratory of Molecular Neuroscience, Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.|Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Esmaeili-Mahani
authors.affiliation: Department of Biotechnology, Shiraz University, Shiraz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Aminizadeh
authors.affiliation: Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mostafavi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009062:Mouth Neoplasms / Q000473:pathology*; D009822:Oils, Volatile / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D046930:Thymus Plant / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D009822:Oils, Volatile; D010936:Plant Extracts; D004317:Doxorubicin