title: Magnetic Resonance Spectroscopy as a Tool for Assessing Macromolecular Structure and Function in Living Cells.
abstract: Investigating the structure, modification, interaction, and function of biomolecules in their native cellular environment leads to physiologically relevant knowledge about their mechanisms, which will benefit drug discovery and design. In recent years, nuclear and electron magnetic resonance (NMR) spectroscopy has emerged as a useful tool for elucidating the structure and function of biomacromolecules, including proteins, nucleic acids, and carbohydrates in living cells at atomic resolution. In this review, we summarize the progress and future of in-cell NMR as it is applied to proteins, nucleic acids, and carbohydrates.
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Conggang
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Jiajing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Guohua
authors.initials: G
authors.lastname: Xu
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Zeting
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Department of Biochemistry and Biophysics, and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599.
authors.forename: Gary
authors.initials: G
authors.lastname: Pielak
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; email: conggangli@wipm.ac.cn , ml.liu@wipm.ac.cn.
authors.forename: Maili
authors.initials: M
authors.lastname: Liu
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D046188:Protein Modification, Translational; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D009696:Nucleic Acids; D004364:Pharmaceutical Preparations; D011506:Proteins
keywords: RNA; in-cell NMR; posttranslational modification; protein interaction; protein structure