title: Visual detection and differentiation of Classic Swine Fever Virus strains using nucleic acid sequence-based amplification (NASBA) and G-quadruplex DNAzyme assay.
abstract: The split G-quadruplex DNAzyme has emerged as a valuable tool for visual DNA detection. Here, we successfully integrated colorimetric split G-quadruplex DNAzyme assay with nucleic acid sequence-based amplification to generate a novel detection approach, allowing visual and rapid detection for the RNA of Shimen and HCLV strains of Classic Swine Fever Virus (CSFV). CSFV is a RNA virus that causes a highly contagious disease in domestic pigs and wild boar. With this method, we were able to detect as little as 10 copies/ml of CSF viral RNA within 3 h in serum samples taken from the field. No interference was encountered in the amplification and detection of Classic Swine Fever Virus in the presence of non-target RNA or DNA. Moreover, Shimen and HCLV strains of Classic Swine Fever Virus could be easily differentiated using the NASBA-DNAzyme system. These findings indicate the NASBA-DNAzyme system is a rapid and practical technique for detecting and discriminating CSFV strains and may be applied to the detection of other RNA viruses.
authors.affiliation: School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan 430074, P. R. China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Life Sciences, South-central University for Nationalities, Wuhan 430074, P.R. China.
authors.forename: Xueyao
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Life Sciences, South-central University for Nationalities, Wuhan 430074, P.R. China.
authors.forename: Gege
authors.initials: G
authors.lastname: Wu
authors.affiliation: College of Life Sciences, South-central University for Nationalities, Wuhan 430074, P.R. China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Wu
authors.affiliation: College of Life Sciences, South-central University for Nationalities, Wuhan 430074, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Qin
authors.affiliation: College of Life Sciences, South-central University for Nationalities, Wuhan 430074, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D006691:Classical Swine Fever / Q000097:blood / Q000235:genetics / Q000821:virology; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000378:metabolism; D054856:G-Quadruplexes; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics*; D013552:Swine
chemical_list: D012367:RNA, Viral