title: The toxic effects of benzo[a]pyrene on activated mouse T cells in vitro.
abstract: Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon contaminant that is widely present in environmental sources, including food. This study aims to clarify the effects of B[a]P toxicity on activated mouse T cells in vitro. Our results show that B[a]P markedly inhibited Concanavalin A (ConA)-induced T lymphocyte proliferation and suppressed the production of the cytokines Interferon (IFN)-γ, Interleukin (IL)-2 and IL-4. Western blot and protein-DNA interaction assays were used to study how B[a]P affects signal transduction. The results revealed that B[a]P suppressed the ConA-induced activation of the Ca2+/CaM/NFκB and Ca2+/CaM/CaN/NFAT signal transduction pathways. These observations indicate that B[a]P has toxic effects on activated mouse T cells in vitro.
authors.affiliation: a Key Laboratory of Zoonosis , Ministry of Education, College of Veterinary Medicine, Jilin University , Changchun , People's Republic of China.|b College of Food Science and Engineering, Jilin University , Changchun , People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Guan
authors.affiliation: b College of Food Science and Engineering, Jilin University , Changchun , People's Republic of China.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: b College of Food Science and Engineering, Jilin University , Changchun , People's Republic of China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Feng
authors.affiliation: b College of Food Science and Engineering, Jilin University , Changchun , People's Republic of China.
authors.forename: Linli
authors.initials: L
authors.lastname: Xu
authors.affiliation: c Department of Pharmacology , College of Basic Medical Science, Jilin University , Changchun , China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Jin
authors.affiliation: a Key Laboratory of Zoonosis , Ministry of Education, College of Veterinary Medicine, Jilin University , Changchun , People's Republic of China.|b College of Food Science and Engineering, Jilin University , Changchun , People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D020013:Calcium Signaling / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology
chemical_list: D016207:Cytokines; D003208:Concanavalin A; D001564:Benzo(a)pyrene
keywords: B[a]P; NF-κB; NFAT; T-cell; toxic effect