title: Selective fluorescence sensing and quantification of uric acid by naphthyridine-based receptor in biological sample.
abstract: Naphthyridine-based fluorescent probe H1 was synthesized and characterized for the quantification and selective detection of Uric Acid (UA) in live cell. In presence of UA, H1 forms the specific host-guest complex mainly through intermolecular hydrogen bonding and aromatic stacking which produces "turn-off" fluorescence. The probe and UA is found to be 1:1 stoichiometry on the basis of absorption and fluorescence titrations. The probe H1 has been shown to detect UA up to 0.6µM at pH 7.4. DFT-TDDFT calculations were performed in order to demonstrate the sensing mechanism and the electronic properties of the receptor-donor complex. The selectivity was evaluated in Vero cells in the presence of UA with other purine derivatives, structurally similar to UA. It was found to exhibit no cytotoxicity effect in tested concentration of H1 and good membrane permeability for the detection of UA in living cell system. The unknown concentration of UA in serum and urine can be measured easily using the fluorescence property of probe H1.
authors.affiliation: Department of Chemistry, Visva-Bharati, Santiniketan-731235, West Bengal, India. Electronic address: prithidipa@hotmail.com.
authors.forename: Prithidipa
authors.initials: P
authors.lastname: Sahoo
authors.affiliation: Department of Chemistry, Visva-Bharati, Santiniketan-731235, West Bengal, India.
authors.forename: Sujoy
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Chemistry, Visva-Bharati, Santiniketan-731235, West Bengal, India.
authors.forename: Himadri Sekhar
authors.initials: HS
authors.lastname: Sarkar
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711103, India.
authors.forename: Kalipada
authors.initials: K
authors.lastname: Maiti
authors.affiliation: Department of Microbiology, University of Calcutta, Kolkata 700019, India.
authors.forename: Md Raihan
authors.initials: MR
authors.lastname: Uddin
authors.affiliation: Department of Microbiology, University of Calcutta, Kolkata 700019, India.
authors.forename: Sukhendu
authors.initials: S
authors.lastname: Mandal
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D008958:Models, Molecular; D009287:Naphthyridines / Q000737:chemistry*; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence; D014527:Uric Acid / Q000032:analysis*; D014709:Vero Cells
chemical_list: D005456:Fluorescent Dyes; D009287:Naphthyridines; D014527:Uric Acid
keywords: DFT study; Fluorescence “On-Off”; Live cell imaging; Naphthyridine-based chemosensor; Quantification; Uric acid