title: Cloning, sequencing and characterization of the genome of a recombinant norovirus of the rare genotype GII.P7/GII.6 in China.
abstract: The genome sequence of a rare recombinant norovirus (NoV) genotype obtained from clinical samples in China was determined using one-step reverse transcription PCR. It was identified as the GII.P7/GII.6 genotype using both phylogenetic and SimPlot analyses. A high degree of variability was observed in the P2 subdomain, especially in the B-loop structure. The recombination breakpoints of all available GII.P7/GII.6 strains were mapped to two different positions within the RdRp region, both of which were at least 40 nt upstream of the overlap of ORF1 and 2. The GII.P7/GII.6 genotype appears to have been circulating in Asia for at least 10 years.
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cai
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.|Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.|Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.|Institute of Biochemistry and Molecular Cell Biology, University of Göttingen, Göttingen, Germany.
authors.forename: Shuling
authors.initials: S
authors.lastname: Yan
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai, China. yjwang@shou.edu.cn.|Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China. yjwang@shou.edu.cn.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D017250:Caliciviridae Infections / Q000821:virology*; D002681:China; D003001:Cloning, Molecular; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008297:Male; D029322:Norovirus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA