title: Central roles of iron in the regulation of oxidative stress in the yeast Saccharomyces cerevisiae.
abstract: Oxygen is essential for aerobic organisms but causes cytotoxicity probably through the generation of reactive oxygen species (ROS). In this study, we screened for the genes that regulate oxidative stress in the yeast Saccharomyces cerevisiae, and found that expression of CTH2/TIS11 caused an increased resistance to ROS. CTH2 is up-regulated upon iron starvation and functions to remodel metabolism to adapt to iron starvation. We showed here that increased resistance to ROS by CTH2 would likely be caused by the decreased ROS production due to the decreased activity of mitochondrial respiration, which observation is consistent with the fact that CTH2 down-regulates the mitochondrial respiratory proteins. We also found that expression of CTH1, a paralog of CTH2, also caused an increased resistance to ROS. This finding supported the above view, because mitochondrial respiratory proteins are the common targets of CTH1 and CTH2. We further showed that supplementation of iron in medium augmented the growth of S. cerevisiae under oxidative stress, and expression of CTH2 and supplementation of iron collectively enhanced its growth under oxidative stress. Since CTH2 is regulated by iron, these findings suggested that iron played crucial roles in the regulation of oxidative stress in S. cerevisiae.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Matsuo
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Mizobuchi
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.
authors.forename: Maya
authors.initials: M
authors.lastname: Nakashima
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Miki
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan. mifuji@yokohama-cu.ac.jp.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051816:Tristetraprolin / Q000235:genetics / Q000378:metabolism
chemical_list: C102098:CTH1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; C091790:TIS11 protein, S cerevisiae; D014157:Transcription Factors; D051816:Tristetraprolin; D007501:Iron
keywords: CTH2/TIS11; Iron; Oxidative stress; Paraquat; Respiration; Saccharomyces cerevisiae