title: Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts.
abstract: OBJECTIVE
  To investigate in human dental pulp fibroblasts (HDPF) the expression of factors involved in dental pulp physiopathological processes and in an experimental model of cell activation called nemosis, and to compare the behaviour of pulp cell activation with sound lung fibroblast MRC5, employed as a reference model for nemosis.
  
  
  METHODS
  Nemotic response was induced in three-dimensional cultures of HDPF and lung fibroblasts. The expressions of molecules involved in physiological (alkaline phosphatase, type I collagen) and in inflammatory processes (IL-6, CXCL8, CCL20, COX-2) were studied using real-time PCR. Concentrations of IL-6 and CXCL8 were analysed during 4 days with ELISA. Nonparametric tests were used to determine statistical differences between groups.
  
  
  RESULTS
  A significant decrease (P < 0.001) in type I collagen and alkaline phosphatase was observed in MRC5 and HDPF nemotic responses. Although the amounts of mRNA differed between these cell types, there was an increase in CCL20, CXCL8 and COX-2 expression (P < 0.001). Unlike HDPF, MRC5 spheroids displayed significant amounts of IL-6 concentrations and mRNA expression. Notably, increased concentrations of CXCL8 were recorded in all three-dimensional cultures compared with monolayers as a function of time (P < 0.05).
  
  
  CONCLUSIONS
  Although the nemotic responses observed were not identical in the pulpal and lung fibroblasts, similarities occurred in the expression of chemokines and cyclooxygenase-2. Nemotic reactions and inflammatory processes in pulp diseases share similarities in terms of the expression of factors. Thus, this in vitro model could constitute a powerful tool to study intercellular relations within the dental pulp and to develop new local treatments to counteract the inflammatory reaction that occurs during pulpitis.
authors.affiliation: Faculté d'Odontologie, Equipe Verres et Céramiques, UMR CNRS 6226, Institut des Sciences Chimiques de Rennes, Université de Rennes 1, Rennes, France.|Service d'Odontologie Conservatrice et Endodontie, Centre Hospitalier Universitaire, Rennes, France.
authors.forename: J
authors.identifier: http://orcid.org/0000-0001-6025-8167
authors.initials: J
authors.lastname: Le Clerc
authors.affiliation: Faculté d'Odontologie, Equipe Verres et Céramiques, UMR CNRS 6226, Institut des Sciences Chimiques de Rennes, Université de Rennes 1, Rennes, France.
authors.forename: S
authors.initials: S
authors.lastname: Tricot-Doleux
authors.affiliation: Faculté d'Odontologie, Equipe Verres et Céramiques, UMR CNRS 6226, Institut des Sciences Chimiques de Rennes, Université de Rennes 1, Rennes, France.
authors.forename: P
authors.initials: P
authors.lastname: Pellen-Mussi
authors.affiliation: Faculté d'Odontologie, Equipe Verres et Céramiques, UMR CNRS 6226, Institut des Sciences Chimiques de Rennes, Université de Rennes 1, Rennes, France.|Service d'Odontologie Conservatrice et Endodontie, Centre Hospitalier Universitaire, Rennes, France.
authors.forename: M
authors.initials: M
authors.lastname: Pérard
authors.affiliation: Faculté d'Odontologie, Equipe Verres et Céramiques, UMR CNRS 6226, Institut des Sciences Chimiques de Rennes, Université de Rennes 1, Rennes, France.|Service de Parodontologie, Centre Hospitalier Universitaire, Rennes, France.
authors.forename: S
authors.initials: S
authors.lastname: Jeanne
authors.affiliation: Service d'Odontologie Conservatrice et Endodontie, Centre Hospitalier Universitaire, Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Pérez
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D016923:Cell Death / Q000502:physiology*; D054418:Chemokine CCL20 / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D054418:Chemokine CCL20; D024042:Collagen Type I; D015850:Interleukin-6; D016209:Interleukin-8; D051546:Cyclooxygenase 2; D000469:Alkaline Phosphatase
keywords: dental pulp diseases; dental pulp fibroblasts; inflammation; nemosis; programmed necrosis; spheroids