title: Optical Projection Tomography as a Novel Method to Visualize and Quantitate Whole-Brain Patterns of Cell Proliferation in the Adult Zebrafish Brain.
abstract: How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using FIJI and IMARIS software.
authors.affiliation: Australian Regenerative Medicine Institute, Monash University , Clayton, Australia .
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Lindsey
authors.affiliation: Australian Regenerative Medicine Institute, Monash University , Clayton, Australia .
authors.forename: Jan
authors.initials: J
authors.lastname: Kaslin
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000254:growth & development; D049109:Cell Proliferation; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software; D041622:Tomography, Optical / Q000379:methods / Q000662:veterinary*; D015027:Zebrafish / Q000033:anatomy & histology* / Q000254:growth & development
keywords: brain development; clearing; neurogenesis; regeneration; stem cell proliferation; three-dimensional imaging