title: Attempt to produce a chick/mouse heteroclass musculature.
abstract: Heteroclass chick/mouse chimaeras were prepared by transplanting somitic presumptive myogenic cells or limb bud myoblasts from donor mouse embryos into chick hosts, to replace (1) previously extirpated brachial somitic mesoderm or (2) experimentally deleted limb premuscular masses. Since mouse and chick cells can be distinguished by differential staining affinities, this parameter was used to verify the viability of the implant and to assess its fate. Our analyses showed that transplanted mouse somitic myogenic stem cells or limb bud myoblasts did not participate in the host brachial musculature, whatever the experimental conditions.
authors.affiliation: Unité Associée au CNRS n 682 "Morphogenèse expérimentale", Département de Biologie, Université de Grenoble I, BP68, F-38402, Saint Martin d'Hères, France.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Kieny
authors.affiliation: Unité Associée au CNRS n 682 "Morphogenèse expérimentale", Département de Biologie, Université de Grenoble I, BP68, F-38402, Saint Martin d'Hères, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Chevallier
authors.affiliation: Unité Associée au CNRS n 682 "Morphogenèse expérimentale", Département de Biologie, Université de Grenoble I, BP68, F-38402, Saint Martin d'Hères, France.
authors.forename: Marie Paule
authors.initials: MP
authors.lastname: Pautou
keywords: Embryonic chick/mouse chimaeras; Muscle ontogenesis; Premuscular mass transplants; Somite exchange