title: Application of a Nanostructured Enzymatic Biosensor Based on Fullerene and Gold Nanoparticles to Polyphenol Detection.
abstract: Electrochemical biosensors provide an attractive means of analyzing the content of a biological sample due to the direct conversion of a biological event to an electronic signal. The signal transduction and the general performance of electrochemical biosensors are often determined by the surface architectures that connect the sensing element to the biological sample at the nanometer scale. The most common surface modification techniques, the various electrochemical transduction mechanisms, and the choice of the recognition receptor molecules all influence the ultimate sensitivity of the sensor. We show herein a novel electrochemical biosensing platform based on the coupling of two different nanostructured materials (gold nanoparticles and fullerenols) displaying interesting electrochemical features. The use of these nanomaterials improved the electrochemical performance of the proposed biosensor.An application of the nanostructured enzyme-based biosensor has been developed for evaluating the detection of polyphenols either in buffer solution or in real wine samples.
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5, 00185, Roma, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Tortolini
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5, 00185, Roma, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sanzò
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5, 00185, Roma, Italy.
authors.forename: Riccarda
authors.initials: R
authors.lastname: Antiochia
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5, 00185, Roma, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 5, 00185, Roma, Italy. gabriele.favero@uniroma1.it.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002138:Calibration; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004798:Enzymes / Q000737:chemistry; D004867:Equipment Design; D037741:Fullerenes / Q000737:chemistry; D005707:Gallic Acid / Q000032:analysis; D006046:Gold / Q000737:chemistry; D042845:Laccase / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D059808:Polyphenols / Q000032:analysis*; D014920:Wine / Q000032:analysis
chemical_list: D004798:Enzymes; D037741:Fullerenes; D059808:Polyphenols; D005707:Gallic Acid; D006046:Gold; D042845:Laccase
keywords: Direct electron transfer; Enzymatic biosensor; Laccase; Nanomaterials; Polyphenols