title: Phytotoxic activity and chemical composition of Cassia absus seeds and aerial parts.
abstract: The present study was conducted to assess the phytotoxic potential and the phytochemical composition of Cassia absus. Aqueous extracts caused significant reduction in root growth of Lactuca sativa. Seed extract was more effective than aerial part extract. Successive extractions of this plant were performed using solvents with increasing polarities. The methanolic seed extract exerted strong phytotoxic effect on seedling growth, followed by petroleum ether extract of the aerial part. The phytochemical investigation showed that among the organic extracts, methanol extracts of seeds and aerial parts contained the highest amounts of total phenolics and proanthocyanidins. Seeds were rich in linoleic acid followed by palmitic acids. Palmitic, stearic and arachidic acids were the major fatty acids in aerial parts. HPLC-DAD analysis of the methanolic extracts revealed the presence of luteolin in C. absus aerial parts.
authors.affiliation: a Higher Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.|b Department of Biological Sciences and Plant Protection, Higher Institute of Agronomy of Chott Meriem , University of Sousse , Chott-Mariem , Tunisia.
authors.forename: I
authors.initials: I
authors.lastname: Zribi
authors.affiliation: b Department of Biological Sciences and Plant Protection, Higher Institute of Agronomy of Chott Meriem , University of Sousse , Chott-Mariem , Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Sbai
authors.affiliation: a Higher Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.|b Department of Biological Sciences and Plant Protection, Higher Institute of Agronomy of Chott Meriem , University of Sousse , Chott-Mariem , Tunisia.
authors.forename: N
authors.initials: N
authors.lastname: Ghezal
authors.affiliation: c General and Organic Chemistry Unit, Gembloux Agro-Bio Tech , University of Liège , Gembloux , Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Richard
authors.affiliation: c General and Organic Chemistry Unit, Gembloux Agro-Bio Tech , University of Liège , Gembloux , Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Trisman
authors.affiliation: c General and Organic Chemistry Unit, Gembloux Agro-Bio Tech , University of Liège , Gembloux , Belgium.
authors.forename: M L
authors.initials: ML
authors.lastname: Fauconnier
authors.affiliation: b Department of Biological Sciences and Plant Protection, Higher Institute of Agronomy of Chott Meriem , University of Sousse , Chott-Mariem , Tunisia.
authors.forename: R
authors.initials: R
authors.lastname: Haouala
mesh_terms: D002366:Cassia / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D010636:Phenols / Q000032:analysis; D064209:Phytochemicals / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D044945:Proanthocyanidins / Q000032:analysis; D012639:Seeds / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts; D044945:Proanthocyanidins; D012997:Solvents
keywords: Cassia absus; GC-FID; WESIA; phytochemicals; phytotoxicity