title: A comparison of transcriptomic profiles in endometrium during window of implantation between women with unexplained recurrent implantation failure and recurrent miscarriage.
abstract: The endometrium becomes receptive to the embryo only in the mid-luteal phase, but not in the other stages of the menstrual cycle. Endometrial factors play an important role in implantation. Women with recurrent miscarriage and recurrent implantation failure have both been reported to have altered expression of receptivity markers during the window of implantation. We aimed to compare the gene expression profiles of the endometrium in the window of implantation among women with unexplained recurrent implantation failures (RIF) and unexplained recurrent miscarriages (RM) by RNA sequencing (RNA-Seq). In total 20 patients (9 RIF and 11 RM) were recruited. In addition 4 fertile subjects were included as reference. Endometrium samples were precisely timed on the 7th day after luteal hormone surge (LH + 7). All the 24 endometrium samples were extracted for total RNA. The transcriptome was determined by RNA-Seq in the first 14 RNA samples (5 RIF, 6 RM and 3 fertile). Differentially expressed genes between RM and RIF were validated by quantitative real-time PCR (qPCR) in all 24 RNA samples (9 RIF, 11 RM and 4 fertile). Transcriptomic profiles of RM and RIF, but not control samples, were separated from each other by principle component analysis (PCA) and support vector machine (SVM). Complementary and coagulation cascades pathway was significantly up-regulated in RIF while down-regulated in RM. Differentially expressed genesC3, C4, C4BP, DAF, DFandSERPING1in complement and coagulation cascade pathway between RM and RIF were further validated by qPCR. This study compared endometrial transcriptome among patients with RIF and RM in the window of implantation; it identified differential molecular pathways in endometrium between RIF and RM, which potentially affect the implantation process.
authors.affiliation: Department of Obstetrics and Gynaecology.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Life SciencesThe Chinese University of Hong KongHong Kong SAR, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Obstetrics and Gynaecology.|Reproductive Medicine Centre of the Affiliated HospitalGuilin Medical University, Guilin, Guangxi, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynaecology.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynaecology.|Reproductive Medicine Centre of the Affiliated HospitalGuilin Medical University, Guilin, Guangxi, China.
authors.forename: Jiamiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Biomolecular Sciences Research CentreSheffield Hallam University, Sheffield, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Laird
authors.affiliation: Department of Obstetrics and Gynaecology.|Li Ka Shing Institute of Health Sciences.|School of Biomedical SciencesThe Chinese University of Hong Kong, Hong Kong SAR, China.
authors.forename: Chi Chiu
authors.initials: CC
authors.lastname: Wang
authors.affiliation: School of Life SciencesThe Chinese University of Hong KongHong Kong SAR, China.
authors.forename: Ting Fung
authors.initials: TF
authors.lastname: Chan
authors.affiliation: Department of Obstetrics and Gynaecology tinchiu.li@cuhk.edu.hk.
authors.forename: Tin Chiu
authors.initials: TC
authors.lastname: Li
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics* / Q000473:pathology; D000328:Adult; D002681:China / Q000453:epidemiology; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology*; D015995:Prevalence; D012008:Recurrence; D059467:Transcriptome; D017211:Treatment Failure
chemical_list: D007986:Luteinizing Hormone