title: Quantum-dot-based suspension microarray for multiplex detection of lung cancer markers: preclinical validation and comparison with the Luminex xMAP®system.
abstract: A novel suspension multiplex immunoassay for the simultaneous specific detection of lung cancer markers in bronchoalveolar lavage fluid (BALF) clinical samples based on fluorescent microspheres having different size and spectrally encoded with quantum dots (QDEM) was developed. The designed suspension immunoassay was validated for the quantitative detection of three lung cancer markers in BALF samples from 42 lung cancer patients and 10 control subjects. Tumor markers were detected through simultaneous formation of specific immune complexes consisting of a capture molecule, the target antigen, and biotinylated recognition molecule on the surface of the different QDEM in a mixture. The immune complexes were visualized by fluorescently labeled streptavidin and simultaneously analyzed using a flow cytometer. Preclinical validation of the immunoassay was performed and results were compared with those obtained using an alternative 3-plex immunoassay based on Luminex xMAP®technology, developed on classical organic fluorophores. The comparison showed that the QDEM and xMAP®assays yielded almost identical results, with clear discrimination between control and clinical samples. Thus, developed QDEM technology can become a good alternative to xMAP®assays permitting analysis of multiple protein biomarkers using conventional flow cytometers.
authors.affiliation: Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russian Federation.
authors.forename: Regina
authors.initials: R
authors.lastname: Bilan
authors.affiliation: Department of Research and Development, Progenika Biopharma S.A., Derio, 48160 Spain.
authors.forename: Amagoia
authors.initials: A
authors.lastname: Ametzazurra
authors.affiliation: Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russian Federation.
authors.forename: Kristina
authors.initials: K
authors.lastname: Brazhnik
authors.affiliation: Department of Research and Development, Progenika Biopharma S.A., Derio, 48160 Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Escorza
authors.affiliation: Department of Research and Development, Progenika Biopharma S.A., Derio, 48160 Spain.
authors.forename: David
authors.initials: D
authors.lastname: Fernández
authors.affiliation: Department of Research and Development, Progenika Biopharma S.A., Derio, 48160 Spain.
authors.forename: María
authors.initials: M
authors.lastname: Uríbarri
authors.affiliation: Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russian Federation.|Laboratoire de Recherche en Nanosciences, LRN - EA4682, Université de Reims Champagne-Ardenne, 51096 Reims, France.
authors.forename: Igor
authors.initials: I
authors.lastname: Nabiev
authors.affiliation: Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russian Federation.|Laboratoire de Recherche en Nanosciences, LRN - EA4682, Université de Reims Champagne-Ardenne, 51096 Reims, France.
authors.forename: Alyona
authors.initials: A
authors.lastname: Sukhanova
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D015999:Multivariate Analysis; D045663:Quantum Dots / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor