title: Gab3 overexpression in human glioma mediates Akt activation and tumor cell proliferation.
abstract: This current study tested expression and potential biological functions of Gab3 in human glioma. Gab3 mRNA and protein expression was significantly elevated in human glioma tissues and glioma cells. Its level was however low in normal brain tissues and primary human astrocytes. In both established (U251MG cell line) and primary human glioma cells, Gab3 knockdown by shRNA/siRNA significantly inhibited Akt activation and cell proliferation. Reversely, forced Gab3 overexpression in U251MG cells promoted Akt activation and cell proliferation. In vivo, the growth of U251MG tumors in nude mice was inhibited following expressing Gab3 shRNA. Akt activation in cancer tissues was also suppressed by Gab3 shRNA. Together, we conclude that Gab3 overexpression in human glioma mediates Akt activation and cancer cell proliferation.
authors.affiliation: Department of Neurosurgery, RuiJin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Pifeng
authors.initials: P
authors.lastname: Jia
authors.affiliation: Department of Neurosurgery, RuiJin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, RuiJin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Weiting
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, RuiJin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, RuiJin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yu
authors.identifier: http://orcid.org/0000-0002-1519-910X
authors.initials: Y
authors.lastname: Cai
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D004789:Enzyme Activation; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000378:metabolism*; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051057:Proto-Oncogene Proteins c-akt; D012333:RNA, Messenger; C443673:GAB3 protein, human