title: Family cord blood banking for sickle cell disease: a twenty-year experience in two dedicated public cord blood banks.
abstract: Efforts to implement family cord blood banking have been developed in the past decades for siblings requiring stem cell transplantation for conditions such as sickle cell disease. However, public banks are faced with challenging decisions about the units to be stored, discarded, or used for other endeavors. We report here 20 years of experience in family cord blood banking for sickle cell disease in two dedicated public banks. Participants were pregnant women who had a previous child diagnosed with homozygous sickle cell disease. Participation was voluntary and free of charge. All mothers underwent mandatory serological screening. Cord blood units were collected in different hospitals, but processed and stored in two public banks. A total of 338 units were stored for 302 families. Median recipient age was six years (11 months-15 years). Median collected volume and total nucleated cell count were 91 mL (range 23-230) and 8.6×108(range 0.7-75×108), respectively. Microbial contamination was observed in 3.5% (n=12), positive hepatitis B serology in 25% (n=84), and homozygous sickle cell disease in 11% (n=37) of the collections. Forty-four units were HLA-identical to the intended recipient, and 28 units were released for transplantation either alone (n=23) or in combination with the bone marrow from the same donor (n=5), reflecting a utilization rate of 8%. Engraftment rate was 96% with 100% survival. Family cord blood banking yields good quality units for sibling transplantation. More comprehensive banking based on close collaboration among banks, clinical and transplant teams is recommended to optimize the use of these units.
authors.affiliation: Eurocord, Paris-Diderot University EA 3518, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France hanadi.rafii-elayoubi@aphp.fr.|Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Hanadi
authors.initials: H
authors.lastname: Rafii
authors.affiliation: Department of Pediatrics, Referral Center for Sickle Cell Disease, Centre Hospitalier Intercommunal, Paris XII University, Créteil, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Bernaudin
authors.affiliation: Cell Therapy Facility, EFS Ile de France, Créteil, France.
authors.forename: Helene
authors.initials: H
authors.lastname: Rouard
authors.affiliation: Cell Therapy Facility, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Biotherapy Clinical Investigation Center, Paris-Diderot University, Sorbonne Paris Cité, INSERM, F-75010, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Vanneaux
authors.affiliation: Eurocord, Paris-Diderot University EA 3518, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Ruggeri
authors.affiliation: Biotherapy Department, Necker Children's Hospital, Assistance Publique-Hôpitaux de Paris, France.|Biotherapy Clinical Investigation Center, Groupe Hospitalier Universitaire Ouest, Assistance Publique-Hôpitaux de Paris, INSERM, France.|Paris Descartes-Sorbonne Paris Cité University, Imagine Institute, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Cavazzana
authors.affiliation: Fédération Parisienne Pour le Dépistage et la Prévention des Handicaps de l'Enfant (FPDPHE), Necker Children's Hospital, Assistance Publique-Hôpitaux de Paris, France.
authors.forename: Valerie
authors.initials: V
authors.lastname: Gauthereau
authors.affiliation: Biotherapy Department, Necker Children's Hospital, Assistance Publique-Hôpitaux de Paris, France.|Biotherapy Clinical Investigation Center, Groupe Hospitalier Universitaire Ouest, Assistance Publique-Hôpitaux de Paris, INSERM, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Stanislas
authors.affiliation: Department of Pediatrics, Referral Center for Sickle Cell Disease, Robert Debré Hospital, Assistance Publique-Hôpitaux de Paris, France.
authors.forename: Malika
authors.initials: M
authors.lastname: Benkerrou
authors.affiliation: Department of Pediatrics, Necker Children's Hospital, Assistance Publique-Hôpitaux de Paris, France.
authors.forename: Mariane
authors.initials: M
authors.lastname: De Montalembert
authors.affiliation: Department of Stem Cell Transplantation, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.
authors.forename: Christele
authors.initials: C
authors.lastname: Ferry
authors.affiliation: Department of Hemato-Biology, Tenon Hospital, Assistance Publique-Hôpitaux de Paris, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Girot
authors.affiliation: Department of Pediatrics, Referral Center for Sickle Cell Disease, Centre Hospitalier Intercommunal, Paris XII University, Créteil, France.
authors.forename: Cecile
authors.initials: C
authors.lastname: Arnaud
authors.affiliation: Department of Pediatrics, Referral Center for Sickle Cell Disease, Centre Hospitalier Intercommunal, Paris XII University, Créteil, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Kamdem
authors.affiliation: Department of Gynecology, Centre Hospitalier Intercommunal, Créteil, France.
authors.forename: Joelle
authors.initials: J
authors.lastname: Gour
authors.affiliation: Department of Gynecology, Centre Hospitalier Intercommunal, Créteil, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Touboul
authors.affiliation: Cell Therapy Facility, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Biotherapy Clinical Investigation Center, Paris-Diderot University, Sorbonne Paris Cité, INSERM, F-75010, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Cras
authors.affiliation: Department of Hematology, Groupe Hospitalier Universitaire Henri-Mondor, Créteil, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Kuentz
authors.affiliation: Unité d'Hémovigilance, Groupe Hospitalier Universitaire Henri-Mondor, Créteil, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Rieux
authors.affiliation: Eurocord, Paris-Diderot University EA 3518, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Volt
authors.affiliation: Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Barbara
authors.initials: B
authors.lastname: Cappelli
authors.affiliation: Eurocord, Paris-Diderot University EA 3518, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Karina T
authors.initials: KT
authors.lastname: Maio
authors.affiliation: Eurocord, Paris-Diderot University EA 3518, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Paviglianiti
authors.affiliation: Eurocord, Paris-Diderot University EA 3518, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Chantal
authors.initials: C
authors.lastname: Kenzey
authors.affiliation: Cell Therapy Facility, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Biotherapy Clinical Investigation Center, Paris-Diderot University, Sorbonne Paris Cité, INSERM, F-75010, France.
authors.forename: Jerome
authors.initials: J
authors.lastname: Larghero
authors.affiliation: Eurocord, Paris-Diderot University EA 3518, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris, France.|Monacord, International Observatory for Sickle Cell Disease, Centre Scientifique de Monaco, Monaco.
authors.forename: Eliane
authors.initials: E
authors.lastname: Gluckman
mesh_terms: D000293:Adolescent; D000328:Adult; D000755:Anemia, Sickle Cell / Q000628:therapy*; D001771:Blood Banks / Q000592:standards; D002648:Child; D002675:Child, Preschool; D036101:Cord Blood Stem Cell Transplantation / Q000592:standards*; D005190:Family; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006085:Graft Survival; D006648:Histocompatibility; D006801:Humans; D007223:Infant; D008297:Male; D011247:Pregnancy; D035781:Siblings; D015996:Survival Rate; D014019:Tissue Donors; D055815:Young Adult; D000094902:Blood Banking / Q000379:methods*