title: Effects of SDF-1/CXCR4 on Acute Lung Injury Induced by Cardiopulmonary Bypass.
abstract: Acute lung injury (ALI) is one of the most important complications after cardiopulmonary bypass (CPB) and the complex pathophysiology remains to be resolved incomplete. SDF-1/CXCR4 chemokine axis can chemotactically accumulate inflammatory cell to local tissue and regulate the release of inflammatory factors, and SDF-1 has a strong chemotaxis effect on neutrophils with CXCR4. Since CPB animal model was difficult to establish, there was still no report about the effect of SDF-1/CXCR4 on neutrophil chemotaxis in ALI after CPB. Here, a stable CPB rat model was constructed to clarify the role of SDF-1/CXCR4 axis in the CPB-induced ALI. Real-time quantitative PCR (RT-qPCR), Western blot analysis, and enzyme-linked immunosorbent assay (ELISA) were used to detect the changes of SDF-1 and CXCR4 in lung tissues, blood, bronchoalveolar lavage (BALF), and/or isolated neutrophils. SDF-1/CXCR4 was increased after CPB, both of that were increased in blood; CXCR4 was increased in neutrophils; SDF-1/CXCR4 was also increased in BALF of CPB model. Results indicated that SDF-1/CXCR4 axis played a key role in the process of early ALI after CPB, also showed that lung injury was significantly reduce after blocking SDF-1/CXCR4 axis, suggest that CXCR4 might be a new target for ALI treatment.
authors.affiliation: Department of Cardiothoracic Surgery, The Third People's Hospital of Nantong, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Hai
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Cardiothoracic Surgery, The Third People's Hospital of Nantong, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Rujian
authors.initials: R
authors.lastname: Lu
authors.affiliation: Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China. ntfywangfei@sina.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China. ntliukun@163.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology*; D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D054377:Chemokine CXCL12 / Q000032:analysis / Q000494:pharmacology*; D002633:Chemotaxis; D008297:Male; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000032:analysis / Q000502:physiology*
chemical_list: C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D019718:Receptors, CXCR4
keywords: CXCR4; SDF-1; cardiopulmonary bypass; chemokine; lung injury