title: Abnormal ovarian morphogenesis in Drosophila melanogaster after injection of embryos with conditioned media from the Daudi cell line.
abstract: Drosophila melanogaster embryos were injected before the blastoderm stage with conditioned media from several male Burkitt's lymphoma human cell lines and the Daudi cell line. Such injections do not have any effect on the male genital apparatus or on the female tract. The Daudi conditioned medium modifies the ovarian morphogenesis of the flies and the rudimentary ovaries obtained look like nymphal gonads. Moreover, they have a drastically reduced number of germ cells. The ovaries that looked functional contain numerous necrotic germ cells and the mean number of ovarioles per fly is significantly smaller than that of the controls. The abnormalities observed resemble the results of experimental and genetic lack of germ cells. They disappear at very high dilution (1×10-6).
authors.affiliation: Laboratoire de Physiologie de la Reproduction, Université Pierre et Marie Curie, Bat A, 4 Place Jussieu 75, F-75252, Paris Cedex 05, France.
authors.forename: Noëlle
authors.initials: N
authors.lastname: Richard-Mercier
authors.affiliation: Laboratoire de Zoologie, Université Pierre et Marie Curie, Bat A, 4 Place Jussieu, 75252, Paris Cedex 05, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Thomas-Orillard
authors.affiliation: Unité d'immunogénétique humaine, INSERM U 276, Institut Pasteur, 36 rue du Docteur Roux, F-75015, Paris Cedex, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Fellous
keywords: Daudi conditioned medium; Drosophila; Injection of embryo; Mean number of ovarioles; Rudimentary ovaries