title: The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum.
abstract: The ability of the wheat Lr34 multipathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen Puccinia purpurea and the hemibiotroph Colletotrichum sublineolum, respectively, occurred in transgenic plants expressing the Lr34res ABC transporter. Transgenic sorghum lines that highly expressed the wheat Lr34res gene exhibited immunity to sorghum rust compared to the low-expressing single copy Lr34res genotype that conferred partial resistance. Pathogen-induced pigmentation mediated by flavonoid phytoalexins was evident on transgenic sorghum leaves following P. purpurea infection within 24-72 h, which paralleled Lr34res gene expression. Elevated expression of flavone synthase II, flavanone 4-reductase and dihydroflavonol reductase genes which control the biosynthesis of flavonoid phytoalexins characterized the highly expressing Lr34res transgenic lines 24-h post-inoculation with P. purpurea. Metabolite analysis of mesocotyls infected with C. sublineolum showed increased levels of 3-deoxyanthocyanidin metabolites were associated with Lr34res expression, concomitant with reduced symptoms of anthracnose.
authors.affiliation: CSIRO Agriculture and Food, Canberra, ACT, Australia.
authors.forename: Wendelin
authors.initials: W
authors.lastname: Schnippenkoetter
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Clive
authors.initials: C
authors.lastname: Lo
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, St Lucia, QLD, Australia.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Liu
authors.affiliation: CSIRO Agriculture and Food, Canberra, ACT, Australia.
authors.forename: Katherine
authors.initials: K
authors.lastname: Dibley
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Wai Lung
authors.initials: WL
authors.lastname: Chan
authors.affiliation: Centre for Crop Health, University of Southern Queensland, Toowoomba, QLD, Australia.
authors.forename: Jodie
authors.initials: J
authors.lastname: White
authors.affiliation: CSIRO Agriculture and Food, Canberra, ACT, Australia.
authors.forename: Ricky
authors.initials: R
authors.lastname: Milne
authors.affiliation: CSIRO Data61, Canberra, ACT, Australia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Zwart
authors.affiliation: CSIRO Agriculture and Food, Canberra, ACT, Australia.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Kwong
authors.affiliation: Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Beat
authors.initials: B
authors.lastname: Keller
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, St Lucia, QLD, Australia.
authors.forename: Ian
authors.initials: I
authors.lastname: Godwin
authors.affiliation: Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Simon G
authors.initials: SG
authors.lastname: Krattinger
authors.affiliation: CSIRO Agriculture and Food, Canberra, ACT, Australia.|School of Life and Environmental Sciences, University of Sydney, Sydney, NSW, Australia.
authors.forename: Evans
authors.initials: E
authors.lastname: Lagudah
mesh_terms: D001487:Basidiomycota / Q000472:pathogenicity; D020231:Colletotrichum / Q000472:pathogenicity; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D005419:Flavonoids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D010858:Pigmentation; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D012717:Sesquiterpenes / Q000378:metabolism; D045868:Sorghum / Q000235:genetics*; D014908:Triticum / Q000201:enzymology / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000094162:Phytoalexins
chemical_list: D005419:Flavonoids; D010940:Plant Proteins; D012717:Sesquiterpenes; D003577:Cytochrome P-450 Enzyme System; C114032:cytochrome P-450 CYP93B1 (Glycyrrhiza echinata); D000094162:Phytoalexins
keywords: Lr34; anthracnose; flavonoid phytoalexin; multiple disease resistance; rust