title: The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes.
abstract: Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.
authors.affiliation: a Laboratory of Clinical Pharmacology , Rigshospitalet , Copenhagen , Denmark.
authors.forename: Anne-Sofie
authors.initials: AS
authors.lastname: Sørensen
authors.affiliation: a Laboratory of Clinical Pharmacology , Rigshospitalet , Copenhagen , Denmark.|b Department of Clinical Pharmacology , Bispebjerg and Frederiksberg Hospital , Copenhagen , Denmark.|g Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark.
authors.forename: Laura Kofoed
authors.initials: LK
authors.lastname: Kjær
authors.affiliation: b Department of Clinical Pharmacology , Bispebjerg and Frederiksberg Hospital , Copenhagen , Denmark.
authors.forename: Kasper Meidahl
authors.initials: KM
authors.lastname: Petersen
authors.affiliation: a Laboratory of Clinical Pharmacology , Rigshospitalet , Copenhagen , Denmark.|b Department of Clinical Pharmacology , Bispebjerg and Frederiksberg Hospital , Copenhagen , Denmark.
authors.forename: Trine
authors.initials: T
authors.lastname: Henriksen
authors.affiliation: a Laboratory of Clinical Pharmacology , Rigshospitalet , Copenhagen , Denmark.|b Department of Clinical Pharmacology , Bispebjerg and Frederiksberg Hospital , Copenhagen , Denmark.|g Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark.
authors.forename: Vanja
authors.initials: V
authors.lastname: Cejvanovic
authors.affiliation: c Novo Nordisk Foundation Center for Basic Metabolic Research, Section of Metabolic Genetics, Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark.
authors.forename: Oluf
authors.initials: O
authors.lastname: Pedersen
authors.affiliation: c Novo Nordisk Foundation Center for Basic Metabolic Research, Section of Metabolic Genetics, Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark.
authors.forename: Torben
authors.initials: T
authors.lastname: Hansen
authors.affiliation: d Department of Internal Medicine and Endocrinology , Lillebaelt Hospital , Vejle , Denmark.
authors.forename: Cramer Kjeldahl
authors.initials: CK
authors.lastname: Christensen
authors.affiliation: e Department of Clinical Immunology and Biochemistry , Lillebaelt Hospital , Vejle , Denmark.|f Faculty of Health Sciences, Institute of Regional Health Research, University of Southern Denmark , Odense , Denmark.
authors.forename: Ivan
authors.initials: I
authors.lastname: Brandslund
authors.affiliation: a Laboratory of Clinical Pharmacology , Rigshospitalet , Copenhagen , Denmark.|b Department of Clinical Pharmacology , Bispebjerg and Frederiksberg Hospital , Copenhagen , Denmark.|g Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark.
authors.forename: Henrik Enghusen
authors.initials: HE
authors.lastname: Poulsen
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002853:Chromatography, Liquid / Q000379:methods; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D012907:Smoking; D053719:Tandem Mass Spectrometry
chemical_list: D006151:Guanosine; C046215:8-hydroxyguanosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-oxo-7,8-dihydro-2′-deoxyguanosine; 8-oxo-7,8-dihydroguanosine; 8-oxoGuo; 8-oxodG; diabetes mellitus type 2; oxidative stress; tobacco