title: Role of thymic B cells in the development of thymus-derived regulatory T cell in vitro.
abstract: The thymus contains a low frequency of B cells, about 0.1-0.5% of total population of thymocytes that were shown to contribute in thymic negative selection. The fact that B cells in the periphery have been contributed in the generation of the T regulatory cell compartment emerged the idea that this process may indeed be initiated firstly within the thymus. The results of this study revealed that activated thymic B cells maintained the high percentage of nTreg generation or counteracted the decrease of this percentage observed in non-activated culture, and both activators (LPS and IMQ) have the same effect in the process of nTreg generation by B cells. In addition, the activated cultures showed increase of the level of expression of Foxp3 transcription factor. In this study we confirmed that thymic B cells in the condition of our experiments did not influence the generation of nTregs, but rather maintain their viability when activated by both activators.
authors.affiliation: University of Warsaw, Faculty of Biology, Department of Immunology, Miecznikowa 1, 02-096 Warsaw, Poland; Department of Biology, College of Science, University of Kerbala, Kerbala, Iraq. Electronic address: haidgen@yahoo.com.
authors.forename: Haider H
authors.initials: HH
authors.lastname: Mohammed Ali
authors.affiliation: University of Warsaw, Faculty of Biology, Department of Immunology, Miecznikowa 1, 02-096 Warsaw, Poland.
authors.forename: Nadzieja
authors.initials: N
authors.lastname: Drela
mesh_terms: D000634:Aminoquinolines / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D000077271:Imiquimod; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D060168:Thymocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*
chemical_list: D000634:Aminoquinolines; D015703:Antigens, CD; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D000077271:Imiquimod
keywords: Costimulatory molecules; IMQ; LPS; MHC II; Thymic B cells; nTregs