title: Nitrile-hydrolyzing enzyme from Meyerozyma guilliermondii and its potential in biosynthesis of 3-hydroxypropionic acid.
abstract: 3-Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis. Traditionally, 3-HP was produced by chemical methods and fermentation process. In this work, a novel enzymatic method was developed for green synthesis of 3-HP. A yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-HPN) as the sole nitrogen source. It was identified to be Meyerozyma guilliermondii CGMCC12935 by sequencing of the 18S ribosomal DNA and internal transcribed spacer, together with analysis of the morphology characteristics. The catalytic properties of M. guilliermondii CGMCC12935 resting cells were determined, and the optimum activity was achieved at 55 °C and pH 7.5. The enzyme showed broad substrate specificity towards nitriles, especially 3-HPN, aminoacetonitrile and 3-cyanopyridine. The presence of Ag+, Pb2+and excess substrate inhibited the enzyme activity, whereas 5% (v/v) ethyl acetate had a positive effect on the enzyme activity. M. guilliermondii CGMCC12935 resting cells by addition of 3% glucose could thoroughly hydrolyze 500 mM 3-HPN into 3-HP within 100 h and the maximal accumulative production of 3-HP reached 216.33 mM, which was over twofolds than the control group with no additional glucose. And this work would lay the foundation for biological production of 3-HP in industry.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Gong
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Dong
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Wen-Fang
authors.initials: WF
authors.lastname: Dou
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.|National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Lu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Shi
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China. zhenghxu@jiangnan.edu.cn.|National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. zhenghxu@jiangnan.edu.cn.
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Xu
mesh_terms: D002175:Candida; D019344:Lactic Acid / Q000031:analogs & derivatives; D009570:Nitriles; D011725:Pyridines
chemical_list: D009570:Nitriles; D011725:Pyridines; D019344:Lactic Acid; C031601:hydracrylic acid; C020729:3-cyanopyridine
keywords: 3-Hydroxypropionic acid; 3-Hydroxypropionitrile; Meyerozyma guilliermondii; Nitrile-hydrolyzing enzyme