title: Effects of palmitoyl-KVK-L-ascorbic acid on skin wrinkles and pigmentation.
abstract: Wrinkle formation and abnormal pigmentation are major clinical alterations associated with skin aging. As the aim of our study was to investigate the effects of palmitoyl-KVK-L-ascorbic acid on skin aging, the anti-wrinkle and depigmentation effects of palmitoyl-KVK-L-ascorbic acid were evaluated by measuring collagen expression in dermal fibroblast cells and inhibition of melanogenesis in B16F1 cells, respectively. The anti-aging effect of palmitoyl-KVK-L-ascorbic acid cream was also evaluated against a placebo cream in a clinical trial. Our results confirmed that the expression of type Ι collagen in dermal fibroblast cells treated with palmitoyl-KVK-L-ascorbic acid (0.1-4 μg/mL) increased in a dose-dependent manner. In B16F1 cells, treatment with 20 μg/mL palmitoyl-KVK-L-ascorbic acid reduced the melanin content by approximately 20% compared to alpha-melanocyte stimulating hormone treatment. In the clinical trial, application of palmitoyl-KVK-L-ascorbic acid cream led to an improvement in skin roughness and lightness in 12 and 8 weeks, respectively. Our data show that palmitoyl-KVK-L-ascorbic acid is an effective anti-aging agent that reduces wrinkles and abnormal skin pigmentation.
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Hyeong Mi
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Hyo Sun
authors.initials: HS
authors.lastname: An
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Jung-Soo
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Jung Yun
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Chi Ho
authors.initials: CH
authors.lastname: Choi
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Joo Hyuck
authors.initials: JH
authors.lastname: Lim
authors.affiliation: Celltrion Chemical Research Institute, Yongin, Republic of Korea.
authors.forename: Joon-Hun
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Celltrion Chemical Research Institute, Yongin, Republic of Korea.
authors.forename: Hyunnam
authors.initials: H
authors.lastname: Song
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Celltrion Chemical Research Institute, Yongin, Republic of Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Biotechnology Research Institute, R&D Division, Celltrion, Inc., Incheon, Republic of Korea.
authors.forename: Shin-Jae
authors.initials: SJ
authors.lastname: Chang
authors.affiliation: Department of Dermatology, Seoul Paik Hospital, Inje University College of Medicine, Mareunnae-ro 9, Jung-gu, Seoul, Republic of Korea. sun02ya@naver.com.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009842:Oligopeptides / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000503:physiopathology; D015595:Skin Aging / Q000187:drug effects* / Q000502:physiology; D062327:Skin Lightening Preparations / Q000494:pharmacology*
chemical_list: D009842:Oligopeptides; D062327:Skin Lightening Preparations; D003094:Collagen; D001205:Ascorbic Acid
keywords: L-ascorbic acid; Palmitoyl-KVK; Skin pigmentation; Skin wrinkles