title: "Turn-on" fluorescent probe detection of Ca2+ions and applications to bioimaging.
abstract: Ca2+is intracellular divalent cation with the largest concentration variations and involved in many biological phenomena and often acted as a second messenger in signaling pathway. Therefore, the development of probes for specific Ca2+detection is of great importance. Herein, a novel turn-on fluorescent probe for the detection of Ca2+in MeCN-aqueous medium was designed and synthesized. The probe displayed responses to Ca2+with a fluorescence enhancement at 525nm, accompanying with a distinct fluorescence change from nearly colorless to bright yellow-green. Besides, the probe exhibited a rapid signal response time (within 25s), a good linearity range and a lower detection limit (2.70×10-7M). In addition, the ability of the probe to detect Ca2+in living cells (HeLa cells) via an enhancement of the fluorescence has also been demonstrated.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address: yincx@sxu.edu.cn.
authors.forename: Caixia
authors.initials: C
authors.lastname: Yin
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. Electronic address: huofj@sxu.edu.cn.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Huo
mesh_terms: D002118:Calcium / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D061848:Optical Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium
keywords: Bioimaging; Ca(2+); Detection; Fluorescent probe