title: The integrin-binding defective FGF2 mutants potently suppress FGF2 signalling and angiogenesis.
abstract: We recently found that integrin αvβ3 binds to fibroblast growth factor (FGF)-αvβ31 (FGF1), and that the integrin-binding defective FGF1 mutant (Arg-50 to glutamic acid, R50E) is defective in signalling and antagonistic to FGF1 signalling. R50E suppressed angiogenesis and tumour growth, suggesting that R50E has potential as a therapeutic. However, FGF1 is unstable, and we had to express R50E in cancer cells for xenograft study, since injected R50E may rapidly disappear from circulation. We studied if we can develop antagonist of more stable FGF2. FGF2 is widely involved in important biological processes such as stem cell proliferation and angiogenesis. Previous studies found that FGF2 bound to αvβ3 and antagonists to αvβ3 suppressed FGF2-induced angiogenesis. However, it is unclear how FGF2 interacts with integrins. Here, we describe that substituting Lys-119/Arg-120 and Lys-125 residues in the predicted integrin-binding interface of FGF2 to glutamic acid (the K119E/R120E and K125E mutations) effectively reduced integrin binding to FGF2. These FGF2 mutants were defective in signalling functions (ERK1/2 activation and DNA synthesis) in NIH3T3 cells. Notably they suppressed, FGF2 signalling induced by WT FGF2 in endothelial cells, suggesting that the FGF2 mutants are antagonists. The FGF2 mutants effectively suppressed tube formationin vitro, sprouting in aorta ring assaysex vivoand angiogenesisin vivoThe positions of amino acids critical for integrin binding are different between FGF1 and FGF2, suggesting that they do not interact with integrins in the same manner. The newly developed FGF2 mutants have potential as anti-angiogenic agents and useful tools for studying the role of integrins in FGF2 signalling.
authors.affiliation: Department of Medical Technology, Faculty of Health Sciences, Morinomiya University of Medical Sciences, 1-26-16 Nanko-kita, Suminoe, Osaka 559-8611, Japan.|Department of Molecular Pathology, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Mori
authors.affiliation: Department of Molecular Pathology, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Hatori
authors.affiliation: Department of Molecular Pathology, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Naomasa
authors.initials: N
authors.lastname: Kawaguchi
authors.affiliation: Department of Molecular Pathology, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshinosuke
authors.initials: Y
authors.lastname: Hamada
authors.affiliation: Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California 95817, United States of America.
authors.forename: Tsung-Chieh
authors.initials: TC
authors.lastname: Shih
authors.affiliation: Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California 95817, United States of America.
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California 95817, United States of America.
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
authors.affiliation: Department of Molecular Pathology, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Nariaki
authors.initials: N
authors.lastname: Matsuura
authors.affiliation: Department of Molecular Pathology, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California 95817, United States of America.|Department of Dermatology, School of Medicine, University of California Davis, Sacramento, California 95817, United States of America.|The PhD Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan, R.O.C.
authors.forename: Yoko K
authors.initials: YK
authors.lastname: Takada
authors.affiliation: Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California 95817, United States of America ytakada@ucdavis.edu.|Department of Dermatology, School of Medicine, University of California Davis, Sacramento, California 95817, United States of America.|The PhD Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan, R.O.C.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Takada
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000737:chemistry / Q000378:metabolism*; D020014:K562 Cells; D007700:Kinetics; D051379:Mice; D008958:Models, Molecular; D020125:Mutation, Missense; D041681:NIH 3T3 Cells; D018919:Neovascularization, Physiologic / Q000235:genetics; D011485:Protein Binding; D000072417:Protein Domains; D051381:Rats; D015398:Signal Transduction / Q000235:genetics
chemical_list: D000596:Amino Acids; D039302:Integrin alphaVbeta3; D016222:Fibroblast Growth Factor 2
keywords: angiogenesis; antagonists; fibroblast growth factors; integrins