title: Stage-specific gene expression in asynchronous tetraploid mouse embryos formed by fusion of blastomeres and fertilized eggs.
abstract: Asynchronous tetraploid mouse embryos were generated by electrofusion of fertilized eggs with blastomeres from different cleavage stages. The majority of the cytoplasm was always contributed by the egg. The best development was observed when eggs were fused with 2-cell blastomeres. Both genomes became active in fusion embryos (at least the genes for glucose phosphate isomerase did). Stage-specific protein synthesis seemed to be more adjusted to the developmental stage of the egg's than of the blastomere's genome, but at the 2-cell stage both contributed slightly differently to the protein patterns. Also, the time range of the first appearance of the stage-specific embryonic antigen SSEA-1 was wider in fusion embryos than in controls. It seems that the two genomes are not completely synchronized in these tetraploid embryos, a further indication that, in the mouse, the cytoplasm of fertilized eggs might not be compatible with older embryonic nuclei.
authors.affiliation: Fachbereich Biologic, Philipps-Universität, Postfach 1929, W-3550, Marburg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Eid
authors.affiliation: Fachbereich Biologic, Philipps-Universität, Postfach 1929, W-3550, Marburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Petzoldt
keywords: Asynchronous tetraploidy; Electrofusion; Mouse eggs; Stage-specific gene expression