title: MicroRNA expression profiles in human CD3+T cells following stimulation with anti-human CD3 antibodies.
abstract: BACKGROUND
  Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays.
  
  
  RESULTS
  Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR-301a) were statistically significantly up- or down-regulated relative to untreated cells.
  
  
  CONCLUSIONS
  Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways.
authors.affiliation: Molecular Pathology Graduation Program, Medicine Faculty, University of Brasilia, Brasilia, Brazil.
authors.forename: Isabel Garcia
authors.initials: IG
authors.lastname: Sousa
authors.affiliation: Molecular Pathology Graduation Program, Medicine Faculty, University of Brasilia, Brasilia, Brazil.
authors.forename: Manuela Maragno
authors.initials: MM
authors.lastname: do Almo
authors.affiliation: Molecular Biology Graduation Program, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
authors.forename: Kelly Cristina Rodrigues
authors.initials: KC
authors.lastname: Simi
authors.affiliation: Molecular Biology Graduation Program, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
authors.forename: Maryani Andressa Gomes
authors.initials: MA
authors.lastname: Bezerra
authors.affiliation: Catholic University of Brasília, Brasilia, Brazil.
authors.forename: Rosângela Vieira
authors.initials: RV
authors.lastname: Andrade
authors.affiliation: Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.|Institute for Immunology Investigation, A National Institute of Science and Technology, Brasilia, Brazil.
authors.forename: Andréa Queiroz
authors.initials: AQ
authors.lastname: Maranhão
authors.affiliation: Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil. brigido@unb.br.|Institute for Immunology Investigation, A National Institute of Science and Technology, Brasilia, Brazil. brigido@unb.br.
authors.forename: Marcelo Macedo
authors.identifier: http://orcid.org/0000-0002-7136-7059
authors.initials: MM
authors.lastname: Brigido
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D035683:MicroRNAs
keywords: Anti-CD3; Immunoregulation; Immunosuppression; Recombinant antibody; miRNA