title: Melanin as a natural germ cell marker for intraspecific transplantation experiments in Ambystoma mexicanum (Urodela, Amphibia).
abstract: In urodele amphibians, the lack of a reliable germ cell marker restricts the experimental study of the germ lineage. In the present work, we conducted genetic and histological analyses in order to demonstrate that melanin from oocytes constitutes a germ cell marker available for intraspecific experiments in Ambystoma mexicanum. Then, using this marker, we implanted germ cells from undifferentiated gonads (stage 48) into the blastocoel of host embryos and investigated their fate and determined state. Our results show that, from this stage on, the donor cells do not differentiate into other cell types; therefore, they are restricted in developmental capacity and irreversibly determined as germ cells. On the other hand, exogenous germ cells were found in an isotopic position until the young tail-bud stage, and then were found in an ectopic position; these results suggest that, from the middle tail-bud stage on, an active process contributes to migration of primordial germ cells to the gonadal territory.
authors.affiliation: Laboratoire de Biologie animale, Université Pierre et Marie Curie, 9 quai Saint-Bernard, F-75252, Paris Cedex 05, France.
authors.forename: Michelle
authors.initials: M
authors.lastname: Lesimple
authors.affiliation: Laboratoire de Biologie animale, Université Pierre et Marie Curie, 9 quai Saint-Bernard, F-75252, Paris Cedex 05, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Dournon
authors.affiliation: Laboratoire de Biologie animale, Université Pierre et Marie Curie, 9 quai Saint-Bernard, F-75252, Paris Cedex 05, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Houillon
keywords: Ambystoma mexicanum; Germ cell marker; Germ cells; Melanin; Transplantation