title: Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq.
abstract: Dendrobium officinale L. is an important traditional herb with high commercial value in China. Several bioactive constituents, including polysaccharides and alkaloids, reportedly make major contributions toward the excellent medicinal effect of D. officinale. In this study, the contents of polysaccharides and alkaloids in various organs of D. officinale were measured and compared. We took advantage of transcriptomes from four organs to explore biological mechanisms in the organ-specific distribution of active ingredients in D. officinale. Based on Kyoto Encyclopedia of Genes and Genomes pathways, unigenes related to the enzymes involved in fructose and mannose metabolism and unigenes associated with putative upstream elements of the alkaloid biosynthetic pathway were identified. A large number of candidates, including 35 full-length glycosyltransferase genes and 49 full-length P450 genes, were also identified based on the transcriptome data, and the organ-specific expression pattern of these genes was determined. Furthermore, differential expression of all candidate genes was analyzed in two Dendrobium species, D. nobile L. and D. officinale. The data will supply important clues to exploit useful genes involved in polysaccharide and alkaloid synthesis.
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, China.|Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Chenjia
authors.initials: C
authors.lastname: Shen
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, China.|Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Geratology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, 310007, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Foreign Languages, Zhejiang Gongshang University, Hangzhou, 310018, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, China.|Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Meng
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, China.|Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Jiangjie
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, China. shangguo007@126.com.|Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China. shangguo007@126.com.
authors.forename: Shangguo
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, China. whz62@163.com.|Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China. whz62@163.com.
authors.forename: Huizhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis; D053898:Biosynthetic Pathways; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D031667:Dendrobium / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D016695:Glycosyltransferases / Q000235:genetics; D009928:Organ Specificity; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D011134:Polysaccharides / Q000096:biosynthesis; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D000470:Alkaloids; D010940:Plant Proteins; D011134:Polysaccharides; D003577:Cytochrome P-450 Enzyme System; D016695:Glycosyltransferases