title: Polymorphism in 5' untranslated region of heat-shock protein 70 gene as marker of post-partum anoestrus in Murrah buffaloes.
abstract: The enormous production potential of buffaloes has never been accomplished due to various reproductive insufficiencies. Among them, post-partum anoestrus, a multifactorial disorder, is predominant but any genetic association is yet to be established. This study focused to identify novel polymorphisms in heat-shock protein 70 (HSP70) gene and its possible association with post-partum anoestrus in Murrah buffaloes. A 579-bp fragment from 5' untranslated region of HSP70 gene was amplified by polymerase chain reaction from blood genomic DNA of 614 animals maintained under similar management conditions. In phase-I experiment, custom sequencing and restriction enzyme (RE) digestion of the amplified fragment were performed in 40 buffaloes with similar post-partum oestrous conditions over previous consecutive three or more gestations-20 animals each showing post-partum anoestrus (>120 days after parturition) and normal cyclicity (<65 days after parturition). While in phase-II experiment, herd screening by RE analysis was performed in remaining 574 animals. Four transversions at T-75G, C+31G, T+38G and C+97A and three transition mutations at T-153C, T+33C and A+44G positions were observed. Polymorphism at T+38G site revealed significant (p < .05) variation, where homozygous G was present only in post-partum anoestrous animals while nucleotide T was present randomly in both groups of phase-I animals whereas phase-II experiments revealed homozygous G in 55 animals. Regression analysis in relation to average post-partum interval against genotypic frequencies at T+38G also depicted significant association. HSP70 gene polymorphism at T+38G position can therefore be used as genetic marker for excluding probable post-partum anoestrous buffaloes from herd for breeding programmes.
authors.affiliation: Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Veterinary Biochemistry, Ranchi Veterinary College, Birsa Agricultural University, Kanke, Ranchi, Jharkhand, India.
authors.forename: M
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry and Biochemistry, College of Basic Sciences and Humanities, CCSHAU, Hisar, Haryana, India.
authors.forename: N
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: A K
authors.identifier: http://orcid.org/0000-0003-3614-2599
authors.initials: AK
authors.lastname: Balhara
authors.affiliation: Department of Veterinary Physiology & Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.affiliation: Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: I
authors.initials: I
authors.lastname: Singh
mesh_terms: D020121:5' Untranslated Regions; D000780:Anestrus / Q000235:genetics*; D000818:Animals; D002020:Buffaloes / Q000235:genetics* / Q000502:physiology; D005260:Female; D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D049590:Postpartum Period; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions; D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins