title: Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells.
abstract: Polylactic and glycolic acid nanoparticles (PLGA-NPs), coated with L-ferritin, are exploited for the simultaneous delivery of paclitaxel and an amphiphilic Gd based MRI contrast agent into breast cancer cells (MCF7). L-ferritin has been covalently conjugated to the external surface of PLGA-NPs exploiting NHS activated carboxylic groups. The results confirmed that nanoparticles decorated with L-ferritin have many advantages with respect to both albumin-decorated and nondecorated particles. Ferritin moieties endow PLGA-NPs with targeting capability, exploiting SCARA5 receptors overexpressed by these tumor cells, that results in an increased paclitaxel cytotoxicity. Moreover, protein coating increased nanoparticle stability, thus reducing the fast and aspecific drug release before reaching the target. The theranostic potential of the nanoparticles has been demonstrated by evaluating the signal intensity enhancement on T1-weighted MRI images of labeled MCF7 cells. The results were compared with that obtained with MDA cells used as negative control due to their lower SCARA5 expression.
authors.affiliation: Laboratorio de Química Fina, Instituto de Desarrollo Tecnológico para la Industria Química (INTEC), Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Predio CCT-CONICET , Ruta Nacional 168 Km. 0, 3000 Santa Fe, Argentina.
authors.forename: Ludmila N
authors.initials: LN
authors.lastname: Turino
authors.affiliation: University of Turin , Department of Molecular Biotechnology and Health Sciences, via Nizza 52, 10126, Turin, Italy.|SAET S.p.A , via Torino 213, 10040 Leinì, Turin, Italy.
authors.forename: Maria R
authors.identifier: 0000-0003-2374-6692
authors.initials: MR
authors.lastname: Ruggiero
authors.affiliation: University of Turin , Department of Molecular Biotechnology and Health Sciences, via Nizza 52, 10126, Turin, Italy.
authors.forename: Rachele
authors.initials: R
authors.lastname: Stefanìa
authors.affiliation: University of Turin , Department of Molecular Biotechnology and Health Sciences, via Nizza 52, 10126, Turin, Italy.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Cutrin
authors.affiliation: University of Turin , Department of Molecular Biotechnology and Health Sciences, via Nizza 52, 10126, Turin, Italy.
authors.forename: Silvio
authors.initials: S
authors.lastname: Aime
authors.affiliation: University of Turin , Department of Molecular Biotechnology and Health Sciences, via Nizza 52, 10126, Turin, Italy.
authors.forename: Simonetta
authors.identifier: 0000-0003-2998-5424
authors.initials: S
authors.lastname: Geninatti Crich
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology; D003287:Contrast Media / Q000008:administration & dosage / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D005293:Ferritins / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D017239:Paclitaxel / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011100:Polyglycolic Acid / Q000737:chemistry*; D051117:Scavenger Receptors, Class A
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003287:Contrast Media; D004337:Drug Carriers; C569833:SCARA5 protein, human; D051117:Scavenger Receptors, Class A; D011100:Polyglycolic Acid; D005293:Ferritins; D017239:Paclitaxel