title: Characterization of two complete Isospora mitochondrial genomes from passerine birds: Isospora serinuse in a domestic canary and Isospora manorinae in a yellow-throated miner.
abstract: The genus term Isospora is now applied specifically to parasites of birds, with the term Cystoisospora preferred for parasites which infect mammals. Isospora is a common parasitic coccidian in birds worldwide, especially in passerine birds, in which it can cause systemic coccidiosis. The complete mitochondrial genome sequences from two recently identified Isospora species; Isospora serinuse in a domestic canary and Isospora manorinae in a yellow-throated miner, were sequenced and compared with those of other closely related coccidian species. The complete mitochondrial genome sequence for Isospora serinuse is 6260bp in size and 6223bp for Isospora manorinae. The mitochondrial genomes of Isospora serinuse and Isospora manorinae include three protein-coding genes (COI, COIII and CytB), 19 LSU and 14 SSU rDNA fragments, including one newly identified putative LSU fragment in Isospora sp. The arrangement of coding regions in these two Isospora species were identical to that of available Isospora sp. and Eimeria spp. mitochondrial genomes and the start codon usage for protein coding genes was conservative. Phylogenetic analysis of the mt genome of the two Isospora species based on the three coding regions further support that the monophyletic nature of avian Isospora.
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia. Electronic address: ryang@murdoch.edu.au.
authors.forename: Rongchang
authors.initials: R
authors.lastname: Yang
authors.affiliation: Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia, 6076, Australia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Brice
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Oskam
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: WA State Agricultural Biotechnology Centre, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Frances
authors.initials: F
authors.lastname: Brigg
authors.affiliation: WA State Agricultural Biotechnology Centre, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Dave
authors.initials: D
authors.lastname: Berryman
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D002171:Canaries / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D007549:Isospora / Q000235:genetics / Q000302:isolation & purification*; D046109:Passeriformes; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Isospora manorinae; Isospora serinuse; Mitochondrial genome; Next generation sequencing; Phylogenetic analysis; Sanger sequencing