title: Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study.
abstract: Anesthetics utilized for the immobilization of pregnant mammals are prone to crossing the placental barrier and cause adverse effects to the fetuses. In this study, we develop a facile method employing high performance liquid chromatography (HPLC) for the study of Telazol crossing the placental barrier of pregnant pigs. The method mainly relies on the efficient extraction strategy that includes the mobile phase composed of 10 mM ammonium acetate aqueous solution-acetonitrile (1:4, v/v). When the injected dose of Telazol is 10 mg/kg (5 mg/kg of each constituent drug, zolazepem and tiletamine), zolazepam can cross the placental barrier as it is detected in both uterus and umbilical cord with approximately the same content. Conversely, tiletamine is detected in neither uterus nor umbilical cord, indicating the absence of placental transfer of tiletamine. The different absorption rates of the two dosage-equal compounds by pigs are found to be the main cause of their different abilities to cross the placental barrier.
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Gangshi
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Xie
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Ziyao
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Wuyang
authors.initials: W
authors.lastname: Gu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Xianpeng
authors.initials: X
authors.lastname: Shi
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Tianliang
authors.initials: T
authors.lastname: Tang
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Shengquan
authors.initials: S
authors.lastname: Ai
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Fu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Mengjiao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: De
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P. R. China.
authors.forename: Guangneng
authors.initials: G
authors.lastname: Peng
mesh_terms: D000777:Anesthetics / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary*; D004338:Drug Combinations; D005260:Female; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D013552:Swine / Q000097:blood*; D013992:Tiletamine / Q000097:blood / Q000493:pharmacokinetics*; D014599:Uterus / Q000378:metabolism; D015041:Zolazepam / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D000777:Anesthetics; D004338:Drug Combinations; C006131:tiletamine, zolazepam drug combination; D013992:Tiletamine; D015041:Zolazepam
keywords: HPLC; placental barrier; pregnant pigs; tiletamine; zolazepam