title: Knockdown of Long Non-Coding RNA-ZFAS1 Protects Cardiomyocytes Against Acute Myocardial Infarction Via Anti-Apoptosis by Regulating miR-150/CRP.
abstract: ZFAS1 is one of cardiac-specific or cardiac-related lncRNAs. This study was to explore the functional involvement of ZFAS1 and its regulatory role in AMI. In this study, the models of AMI rat and myocardial cell cultured under hypoxia were made. The expression of ZFAS1 and miR-150 of myocardial infarction tissue or cardiac myocytes was determined by quantitative real time PCR. The regulatory role of ZFAS1 on miR-150 was examined by RNA pull down assay. The effect of miR-150 or ZFAS1 expression on cell viability was analyzed by MTT assay. The relative expression of ZFAS1 in the myocardium infracted zone and border zone was significantly upregulated at 1-48 h of AMI rats, but it downregulated at 1 week and 2 weeks; miR-150 was significantly downregulated at AMI-1-48 h and upregulated at 1 and 2 weeks after model establishment. The result of RNA pull down assay indicated that ZFAS1 could interact directly with miR-150. C-reactive protein (CRP) was regulated by ZFAS1/miR-150 axis and negatively targeted by miR-150. Hypoxia caused the decrease of cell viability and the upregulation of CRP at mRNA and protein levels; whereas this upregulation could be attenuated by miR-150 mimic or si-ZFAS1 in H9C2 cells and cardiomyocytes. Knockdown of ZFAS1 or miR-150 overexpression effectively relieved AMI-induced myocardial infarction in AMI-1 week rats. The ZFAS1/miR-150 axis was involved in the molecular mechanism of AMI induced cardiomyocytes apoptosis via regulating CRP. J. Cell. Biochem. 118: 3281-3289, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Jiangxi Health Vocational College, Nanchang 330006, Jiangxi Province, China.
authors.forename: Dan
authors.identifier: 0000-0002-1550-969X
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zou
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Xiaoshu
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Hong
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Renqiang
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Yunde
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, 330006, Jiangxi Province, China.
authors.forename: Yingzhang
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D015687:Cell Hypoxia; D002460:Cell Line; D055785:Gene Knockdown Techniques; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002352:Carrier Proteins; C030929:Crp protein, rat; C551974:MIRN150 microRNA, rat; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: ACUTE MYOCARDIAL INFARCTION; C-REACTIVE PROTEIN; ZFAS1; miR-150