title: Optimising Terpene Synthesis with Flow Biocatalysis.
abstract: Sesquiterpenes are an important family of natural products, many of which exhibit important pharmaceutical and agricultural properties. They are biosynthesised from farnesyl diphosphate in sesquiterpene synthase catalysed reactions. Here, we report the development of a highly efficient segmented flow system for the enzyme-catalysed continuous flow production of sesquiterpenes. Design of experiment (DoE) methods were used to optimise the performance of the flow biocatalysis, and quantitative yields were achieved by using an operationally simple but highly effective segmented flow system.
authors.affiliation: School of Chemistry Cardiff University Park Place, Main Building CF10 3AT Cardiff UK.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Tang
authors.affiliation: School of Chemistry Cardiff University Park Place, Main Building CF10 3AT Cardiff UK.
authors.forename: Rudolf K
authors.initials: RK
authors.lastname: Allemann
authors.affiliation: School of Chemistry Cardiff University Park Place, Main Building CF10 3AT Cardiff UK.
authors.forename: Thomas
authors.identifier: 0000-0002-8990-0667
authors.initials: T
authors.lastname: Wirth
keywords: Biocatalysis; Enzyme catalysis; Flow chemistry; Segmented flow; Synthetic methods; Terpenoids