title: Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor.
abstract: Reepithelialization and wound closure are the desired outcome for several ulcerative conditions. Such resolution reduces the possibility of wound contamination and maintenance of the injury and improves the reestablishment of tissue morphology and functions. Investigators are seeking adjuvant therapies that can accelerate wound healing and are developing new strategies for clinical applications. This study compared the effects of epidermal growth factor (EGF) application and low-level laser therapy (LLLT) on cultured epithelial cells. Cells were seeded in 24-well plates. After a 24-h incubation, the epithelial cells were either treated with EGF (100 μM in serum-free DMEM for 72 h) or subjected to LLLT (780 nm, 25 mW, 0.5, 1.5, and 3 J/cm2) by three applications every 24 h. Seventy-two hours after cells were treated with EGF or LLLT, cell migration, viability, proliferation, and collagen synthesis were assessed. Cells treated with EGF showed increased cell viability, proliferation, and collagen synthesis compared with those cells that received no treatment. LLLT enhanced cell migration; however, no significant effects of laser irradiation on other cell functions were observed. Comparison of both therapies demonstrated that EGF and LLLT enhanced specific epithelial cell activities related to wound healing.
authors.affiliation: Department of Physiology and Pathology, Araraquara School of Densitry, UNESP, R. Humaita, 1680, Araraquara, SP, Brazil. fergbasso@gmail.com.
authors.forename: Fernanda Gonçalves
authors.initials: FG
authors.lastname: Basso
authors.affiliation: Department of Physiology and Pathology, Araraquara School of Densitry, UNESP, R. Humaita, 1680, Araraquara, SP, Brazil.
authors.forename: Taisa Nogueira
authors.initials: TN
authors.lastname: Pansani
authors.affiliation: Department of Physiology and Pathology, Araraquara School of Densitry, UNESP, R. Humaita, 1680, Araraquara, SP, Brazil.
authors.forename: Lais Medeiros
authors.initials: LM
authors.lastname: Cardoso
authors.affiliation: Department of Physiology and Pathology, Araraquara School of Densitry, UNESP, R. Humaita, 1680, Araraquara, SP, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Citta
authors.affiliation: Department of Physiology and Pathology, Araraquara School of Densitry, UNESP, R. Humaita, 1680, Araraquara, SP, Brazil.
authors.forename: Diana Gabriela
authors.initials: DG
authors.lastname: Soares
authors.affiliation: Department of Pediatric Dentistry, Araraquara School of Densitry, UNESP, Araraquara, SP, Brazil.
authors.forename: Débora Salles
authors.initials: DS
authors.lastname: Scheffel
authors.affiliation: Department of Pediatric Dentistry, Araraquara School of Densitry, UNESP, Araraquara, SP, Brazil.
authors.forename: Josimeri
authors.initials: J
authors.lastname: Hebling
authors.affiliation: Department of Physiology and Pathology, Araraquara School of Densitry, UNESP, R. Humaita, 1680, Araraquara, SP, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: de Souza Costa
mesh_terms: D002465:Cell Movement / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D028022:Low-Level Light Therapy
chemical_list: D004815:Epidermal Growth Factor; D003094:Collagen
keywords: Cell migration; Cell proliferation; Epidermal growth factor; Epithelial cells; Low-level laser therapy