title: Study on external factors affecting egg yolk plasma by asymmetrical flow field-flow fractionation.
abstract: In this work, the ability of asymmetrical flow field-flow fractionation (AF4) coupled with UV-vis, multiangle light scattering (MALS), and quasi-elastic light scattering (QELS) (AF4-UV-MALS-QELS) for monitoring aggregation of components of egg yolk plasma was evaluated. The effect of external factors (i.e., pH, storage conditions, freezing and heat treatments) on the egg yolk plasma was studied. The results reveal that the aggregation mechanism of components of egg yolk plasma during heat and freezing treatment is different. The results suggest that the low density lipoproteins (LDLs) in egg yolk plasma undergo a 'clusters-fusion-gel' process under heat treatment at pH10. The alkaline conditions promote the formation of LDL aggregates. Also, storage conditions play a role in the formation of LDL aggregates. It was found that the hen eggs stored for 7days at room temperature contain less aggregate than those stored at 4°C for the same period. The combination of AF4 with online MALS-QELS provided conformational information in terms of the shape and size distribution of LDL aggregates. AF4-UV-MALS-QELS was proved to be a rapid and gentle method for the separation and characterization of egg yolk plasma aggregates.
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; School of Medicine, Hebei University, Baoding 071002, China. Electronic address: douhaiyang-1984@163.com.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Dou
authors.affiliation: School of Medicine, Hebei University, Baoding 071002, China.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Millet Improvement Center, Key Laboratory of Minor Cereal Crops of Hebei Province, Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Science, Shijiazhuang 050035, China.
authors.forename: Aixia
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Hannam University, Daejeon 34054, Republic of Korea.
authors.forename: Jaeyeong
authors.initials: J
authors.lastname: Choi
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China. Electronic address: shensg@hbu.edu.cn.
authors.forename: Shigang
authors.initials: S
authors.lastname: Shen
authors.affiliation: School of Medicine, Hebei University, Baoding 071002, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Medicine, Hebei University, Baoding 071002, China. Electronic address: lyueq@163.com.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Medicine, Hebei University, Baoding 071002, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ding
authors.affiliation: Department of Chemistry, Hannam University, Daejeon 34054, Republic of Korea. Electronic address: slee@hau.kr.
authors.forename: Seungho
authors.initials: S
authors.lastname: Lee
mesh_terms: D000818:Animals; D002645:Chickens; D000067493:Dynamic Light Scattering; D004530:Egg Yolk / Q000737:chemistry*; D061353:Food Storage / Q000379:methods*; D031521:Fractionation, Field Flow / Q000379:methods*; D005615:Freezing; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D008077:Lipoproteins, LDL / Q000737:chemistry*; D010949:Plasma; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D008077:Lipoproteins, LDL
keywords: Aggregation; Asymmetrical flow field-flow fractionation; Egg yolk plasma; Light scattering; Low density lipoprotein