title: Effect of lyophilized prune extract on hyperhomocysteinemia in mice.
abstract: Altered homocysteine metabolism defined as hyperhomocysteinemia is implicated as pathogenic factor in several cardiovascular diseases and atherosclerosis. The purpose of this study was to investigate the efficacy of prune extract, a good source of phenolic antioxidants, on lowering plasma homocysteine level in male hyperhomocysteinemic mice from average weight of 28 g. The administration of lyophilized prune extract was carried out by intraperitoneal injection one day preceding and one hour before sacrifice of mice. Prune extract decreased significantly plasma homocysteine level, correlated with an increased activity of S-adenosylhomocysteine (SAH) hydrolase and NAD(P)H: quinone oxydoreductase-1 activities. Our results suggest a beneficial effect of prune extract on hyperhomocysteinemia with reduction of homocysteine level by its conversion on to SAH by S-adenosylhomocysteine hydrolase, which is activated by NAD+, a by-product of NAD(P)H: quinone oxydo reductase-1.
authors.affiliation: Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Hayate
authors.initials: H
authors.lastname: Haddadi-Guemghar
authors.affiliation: Univ Paris Diderot, Sorbonne Paris Cité, Unité de Biologie Fonctionnelle et Adaptative (BFA), UMR 8251 CNRS, F-75205 Paris, France.
authors.forename: Asma
authors.initials: A
authors.lastname: Tlili
authors.affiliation: UMR 8601 CNRS, University Paris Descartes, Paris Sorbonne Cité, 75270 Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Dairou
authors.affiliation: AP-HP, Hôpital Européen Georges Pompidou, Service de Biochimie, 75015 Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Paul
authors.affiliation: Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Khodir
authors.initials: K
authors.lastname: Madani
authors.affiliation: Univ Paris Diderot, Sorbonne Paris Cité, Unité de Biologie Fonctionnelle et Adaptative (BFA), UMR 8251 CNRS, F-75205 Paris, France. Electronic address: janel@univ-paris-diderot.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Janel
mesh_terms: D043383:Adenosylhomocysteinase / Q000378:metabolism; D000818:Animals; D002726:Chlorogenic Acid / Q000494:pharmacology; D003541:Cystathionine beta-Synthase / Q000235:genetics; D005260:Female; D005612:Freeze Drying; D006710:Homocysteine / Q000097:blood; D020138:Hyperhomocysteinemia / Q000178:diet therapy* / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D000068242:Prunus domestica / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D006710:Homocysteine; D002726:Chlorogenic Acid; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse; D043383:Adenosylhomocysteinase; D003541:Cystathionine beta-Synthase
keywords: Homocysteine; Mice; NAD(P)H:quinone oxydo reductase-1; Prune extract; S-adenosylhomocysteine hydrolase