title: Aspects of amino acid requirements for in vitro evagination of imaginal leg discs inDrosophila melanogaster.
abstract: A method of staging late third instar larvae on the basis of salivary gland morphology is described. Using this technique, we investigated stage related amino acid requirements forDrosophila leg disc evagination in vitro. It was found that the requirement for glutamine lasted longer than that of proline. The staging technique should help in the detailed exploration of the late 3rd instar time period in order to bridge the gap between biochemistry and morphogenesis in the initiation of disc evagination.
authors.affiliation: Department of Zoology, University of Toronto, 25 Harbord Street, M5S 1A1, Toronto, Ontario, Canada.
authors.forename: Bruce Sean
authors.initials: BS
authors.lastname: Silburt
authors.affiliation: Department of Zoology, University of Toronto, 25 Harbord Street, M5S 1A1, Toronto, Ontario, Canada.
authors.forename: Ellen Wynne
authors.initials: EW
authors.lastname: Rapport
keywords: Glutamine; In vitro evagination; Leg discs; Proline; Salivary Glands