title: The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System.
abstract: Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects.
authors.affiliation: Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza.
authors.forename: Enrica
authors.initials: E
authors.lastname: Bertin
authors.affiliation: Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza.
authors.forename: Martina
authors.initials: M
authors.lastname: Piccoli
authors.affiliation: Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza.
authors.forename: Chiara
authors.initials: C
authors.lastname: Franzin
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital.
authors.forename: Andras
authors.initials: A
authors.lastname: Nagy
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital.
authors.forename: Maria
authors.initials: M
authors.lastname: Mileikovsky
authors.affiliation: Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital.
authors.forename: Paolo
authors.initials: P
authors.lastname: De Coppi
authors.affiliation: Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza; m.pozzobon@irpcds.org.
authors.forename: Michela
authors.initials: M
authors.lastname: Pozzobon
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D005260:Female; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D011247:Pregnancy; D011270:Pregnancy, Animal