title: Optogenetic control with a photocleavable protein, PhoCl.
abstract: To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada.|Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Lohman
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Yevgeniya
authors.initials: Y
authors.lastname: Zhuravlova
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Xiaocen
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Wiens
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Hiofan
authors.initials: H
authors.lastname: Hoi
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.
authors.forename: Sine
authors.initials: S
authors.lastname: Yaganoglu
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.
authors.forename: Manuel A
authors.identifier: http://orcid.org/0000-0002-5189-541X
authors.initials: MA
authors.lastname: Mohr
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.|Alberta Glycomics Centre, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Kitova
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.|Alberta Glycomics Centre, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: John S
authors.initials: JS
authors.lastname: Klassen
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.
authors.forename: Periklis
authors.identifier: http://orcid.org/0000-0002-8367-9332
authors.initials: P
authors.lastname: Pantazis
authors.affiliation: Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada.|Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Roger J
authors.identifier: http://orcid.org/0000-0002-7019-7246
authors.initials: RJ
authors.lastname: Thompson
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Robert E
authors.identifier: http://orcid.org/0000-0003-0604-092X
authors.initials: RE
authors.lastname: Campbell
mesh_terms: D017630:Connexins / Q000235:genetics / Q000378:metabolism; D019020:Directed Molecular Evolution; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D019913:Nuclear Localization Signals / Q000235:genetics; D062308:Optogenetics / Q000379:methods*; D018408:Patch-Clamp Techniques; D010777:Photochemistry / Q000379:methods; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism; D000097573:Red Fluorescent Protein; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D017630:Connexins; D049452:Green Fluorescent Proteins; D018841:HSP90 Heat-Shock Proteins; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D019913:Nuclear Localization Signals; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D000097573:Red Fluorescent Protein; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C492764:PANX1 protein, human