title: Analysis of microsatellite instability in CRISPR/Cas9 editing mice.
abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated (Cas) protein 9 system is a novel and powerful tool which is widely used for genome editing. CRISPR/Cas9 is RNA-guided and can lead to desired genomic modifications. However, whether the CRISPR/Cas9-mediated genome editing causes genomic alterations and genomic instability, such as microsatellite instability (MSI), is still unknown. Here we detected MSI in 21 CRISPR/Cas9 mouse strains using a panel of 42 microsatellite loci which were selected from our previous studies. Surprisingly, MSI occurrence was common in CRISPR/Cas9 modified genome, and most of the strains (19/21, 90.5%) examined showed MSI. Of 42 loci examined, 8 loci (8/42, 19.05%) exhibited MSI in the Cas9 editing mice. The Ttll9 (4/42, 9.5%) were the most unstable strains, and D10Mit3 and D10Mit198 (9/21, 42.9%) were considered to be the most "hot" loci in the Cas9 strains we tested. Through analyzing the mutation of microsatellite loci, we provide new insights into the genomic alterations of CRISPR/Cas9 models and it will help us for a better understanding of this powerful technology.
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Xueyun
authors.initials: X
authors.lastname: Huo
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Du
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China; Department of Laboratory Animal, Capital Medical University, Beijing 100069, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Guo
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Shuangyue
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Basic Medical Science, Capital Medical University, Beijing 100069, China; Department of Laboratory Animal, Capital Medical University, Beijing 100069, China. Electronic address: duduyan@ccmu.edu.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Du
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D000072669:Gene Editing; D008810:Mice, Inbred C57BL; D053842:Microsatellite Instability
chemical_list: D064130:CRISPR-Associated Proteins
keywords: C57BL/6J mice; CRISPR/Cas9; Microsatellite instability (MSI)