title: Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy.
abstract: The interactions between proteins or peptides and inorganic materials lead to several interesting processes. For example, combining proteins with minerals leads to the formation of composite materials with unique properties. In addition, the undesirable process of biofouling is initiated by the adsorption of biomolecules, mainly proteins, on surfaces. This organic layer is an adhesion layer for bacteria and allows them to interact with the surface. Understanding the fundamental forces that govern the interactions at the organic-inorganic interface is therefore important for many areas of research and could lead to the design of new materials for optical, mechanical and biomedical applications. This paper demonstrates a single-molecule force spectroscopy technique that utilizes an AFM to measure the adhesion force between either peptides or amino acids and well-defined inorganic surfaces. This technique involves a protocol for attaching the biomolecule to the AFM tip through a covalent flexible linker and single-molecule force spectroscopy measurements by atomic force microscope. In addition, an analysis of these measurements is included.
authors.affiliation: Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem.
authors.forename: Priyadip
authors.initials: P
authors.lastname: Das
authors.affiliation: Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem.
authors.forename: Tal
authors.initials: T
authors.lastname: Duanias-Assaf
authors.affiliation: Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem; meital.reches@mail.huji.ac.il.
authors.forename: Meital
authors.initials: M
authors.lastname: Reches
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D010455:Peptides; D011506:Proteins