title: The X-ray induced G2arrest in mouse eggs: a maternal effect involving a lack of polypeptide phosphorylation.
abstract: In some strains of mice, eggs when X irradiated during the pronuclear stage, undergo a mitotic block in the G2phase of the first cell cycle and cleave when the second division takes place in controls. The importance of this effect varies considerably with the strain and depends exclusively on the maternal genotype. In previous work, two-dimensional electrophoresis showed that eggs blocked at the one-cell stage after irradiation, undergo the same modifications in polypeptide synthesis as two-cell controls of the same age, except at the time of normal first mitosis, where three polypeptide sets of 30, 35 and 45 kDa appear only in cleaving controls. In the present study, we have found phosphorylations in dividing controls, on polypeptides of 30, 35 and 45 kDa. These phosphorylations are not seen in blocked irradiated eggs.
authors.affiliation: Department of Biology, Nuclear Studies Center, CEN/SCK, Boeretang 200, B-2400, Mol, Belgium.
authors.forename: Simone
authors.initials: S
authors.lastname: Grinfeld
authors.affiliation: Department of Biology, Nuclear Studies Center, CEN/SCK, Boeretang 200, B-2400, Mol, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: Jacquet
authors.affiliation: Department of Biology, Nuclear Studies Center, CEN/SCK, Boeretang 200, B-2400, Mol, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Gilles
authors.affiliation: Department of Biology, Nuclear Studies Center, CEN/SCK, Boeretang 200, B-2400, Mol, Belgium.
authors.forename: Lucile
authors.initials: L
authors.lastname: Baugnet-Mahieu
keywords: Cell cycle; Maternal effect; Mouse egg; Phosphorylation; X irradiation