title: Rationale for Determining the Functional Potency of Mesenchymal Stem Cells in Preventing Regulated Cell Death for Therapeutic Use.
abstract: Mesenchymal stem (stromal) cells (MSCs) are being investigated for treating degenerative and inflammatory disorders because of their reparative and immunomodulatory properties. Intricate mechanisms relate cell death processes with immune responses, which have implications for degenerative and inflammatory conditions. We review the therapeutic value of MSCs in terms of preventing regulated cell death (RCD). When cells identify an insult, specific intracellular pathways are elicited for execution of RCD processes, such as apoptosis, necroptosis, and pyroptosis. To some extent, exacerbated RCD can provoke an intense inflammatory response and vice versa. Emerging studies are focusing on the molecular mechanisms deployed by MSCs to ameliorate the survival, bioenergetics, and functions of unfit immune or nonimmune cells. Given these aspects, and in light of MSC actions in modulating cell death processes, we suggest the use of novel functional in vitro assays to ensure the potency of MSCs for preventing RCD. Such analyses should be associated with existing functional assays measuring the anti-inflammatory capabilities of MSCs in vitro. MSCs selected on the basis of two in vitro functional criteria (i.e., prevention of inflammation and RCD) could possess optimal therapeutic efficacy in vivo. In addition, we underline the implications of these perspectives in clinical studies of MSC therapy, with particular focus on acute respiratory distress syndrome. Stem Cells Translational Medicine 2017;6:713-719.
authors.affiliation: Center for Innovative and Translational Medicine, Kochi Medical School, Kochi University, Kochi, Japan.|Department of Environmental Medicine, Kochi Medical School, Kochi University, Kochi, Japan.
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Naji
authors.affiliation: Center for Innovative and Translational Medicine, Kochi Medical School, Kochi University, Kochi, Japan.|Department of Environmental Medicine, Kochi Medical School, Kochi University, Kochi, Japan.
authors.forename: Narufumi
authors.initials: N
authors.lastname: Suganuma
authors.affiliation: STROMALab, INSERM U1031, EFS Pyrénées-Méditerranée, Université de Toulouse, Toulouse, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Espagnolle
authors.affiliation: Science Research Center, Division of Biological Research, Life Sciences and Functional Materials, Kochi Medical School, Kochi University, Kochi, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Yagyu
authors.affiliation: Center for Innovative and Translational Medicine, Kochi Medical School, Kochi University, Kochi, Japan.
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Baba
authors.affiliation: STROMALab, INSERM U1031, EFS Pyrénées-Méditerranée, Université de Toulouse, Toulouse, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Sensebé
authors.affiliation: STROMALab, INSERM U1031, EFS Pyrénées-Méditerranée, Université de Toulouse, Toulouse, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Deschaseaux
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016923:Cell Death; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*
chemical_list: D015415:Biomarkers
keywords: Cell death; Cellular therapy; Clinical translation; Degenerative disorder; Functional potency; Inflammatory disorder; Mesenchymal stem cells; Selection technologies