title: ABNORMAL INFLORESCENCE MERISTEM1 Functions in Salicylic Acid Biosynthesis to Maintain Proper Reactive Oxygen Species Levels for Root Meristem Activity in Rice.
abstract: Root meristem activity determines root growth and root architecture and consequently affects water and nutrient uptake in plants. However, our knowledge about the regulation of root meristem activity in crop plants is very limited. Here, we report the isolation and characterization of a short root mutant in rice (Oryza sativa) with reduced root meristem activity. This root growth defect is caused by a mutation inABNORMAL INFLORESCENCE MERISTEM1(AIM1), which encodes a 3-hydroxyacyl-CoA dehydrogenase, an enzyme involved in β-oxidation. The reduced root meristem activity ofaim1results from reduced salicylic acid (SA) levels and can be rescued by SA application. Furthermore, reduced SA levels are associated with reduced levels of reactive oxygen species (ROS) inaim1, likely due to increased expression of redox and ROS-scavenging-related genes, whose increased expression is (at least in part) caused by reduced expression of the SA-inducible transcriptional repressors WRKY62 and WRKY76. Like SA, ROS application substantially increased root length and root meristem activity inaim1These results suggest that AIM1 is required for root growth in rice due to its critical role in SA biosynthesis: SA maintains root meristem activity through promoting ROS accumulation by inducing the activity of WRKY transcriptional repressors, which repress the expression of redox and ROS-scavenging genes.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China.|Key Laboratory of Plant Nutrition and Fertilizers, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
authors.forename: Lei
authors.identifier: http://orcid.org/0000-0003-4157-0686
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China.|Key Laboratory of Plant Nutrition and Fertilizers, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Nutrition and Fertilizers, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Ruan
authors.affiliation: The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Minjuan
authors.initials: M
authors.lastname: Deng
authors.affiliation: The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Peng
authors.affiliation: College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Nutrition and Fertilizers, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, China yikeke@gmail.com.|The State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Keke
authors.identifier: http://orcid.org/0000-0002-3916-477X
authors.initials: K
authors.lastname: Yi
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018519:Meristem / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species