title: Genetically engineered Escherichia coli Nissle 1917 synbiotic counters fructose-induced metabolic syndrome and iron deficiency.
abstract: Consumption of fructose leads to metabolic syndrome, but it is also known to increase iron absorption. Present study investigates the effect of genetically modified Escherichia coli Nissle 1917 (EcN) synbiotic along with fructose on non-heme iron absorption. Charles foster rats weighing 150-200 g were fed with iron-deficient diet for 2 months. Probiotic treatment of EcN (pqq) and EcN (pqq-glf-mtlK) was given once per week, 109cells after 2 months with fructose in drinking water. Iron levels, blood, and liver parameters for oxidative stress, hyperglycemia, and dyslipidemia were estimated. Transferrin-bound iron levels in the blood decreased significantly after 10 weeks of giving iron-deficient diet. Probiotic treatment of EcN (pqq-glf-mtlK) and fructose together led to the restoration of normal transferrin-bound iron levels and blood and hepatic antioxidant levels as compared to iron-deficient control group. The probiotic also led to the restoration of body weight along with levels of serum and hepatic lipid, blood glucose, and antioxidant in the blood and liver as compared to iron-deficient control group. Restoration of liver injury marker enzymes was also seen. Administration of EcN-producing PQQ and mannitol dehydrogenase enzyme together with fructose led to increase in the transferrin-bound iron levels in the blood and amelioration of consequences of metabolic syndrome caused due to fructose consumption.
authors.affiliation: Molecular Microbial Biochemistry Laboratory (M.M.B.L.), Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India.
authors.forename: Archana Somabhai
authors.initials: AS
authors.lastname: Chaudhari
authors.affiliation: Molecular Microbial Biochemistry Laboratory (M.M.B.L.), Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India.
authors.forename: Ruma
authors.initials: R
authors.lastname: Raghuvanshi
authors.affiliation: Molecular Microbial Biochemistry Laboratory (M.M.B.L.), Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India. gnaresh_k@yahoo.co.in.
authors.forename: G Naresh
authors.initials: GN
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001835:Body Weight; D004032:Diet; D050171:Dyslipidemias / Q000628:therapy; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005632:Fructose / Q000008:administration & dosage* / Q000378:metabolism; D005818:Genetic Engineering; D006943:Hyperglycemia / Q000628:therapy; D007501:Iron / Q000097:blood; D000090463:Iron Deficiencies; D008099:Liver / Q000378:metabolism; D008354:Mannitol Dehydrogenases / Q000378:metabolism; D024821:Metabolic Syndrome / Q000503:physiopathology / Q000628:therapy*; D018384:Oxidative Stress; D045542:PQQ Cofactor / Q000008:administration & dosage*; D019936:Probiotics; D051381:Rats; D058616:Synbiotics; D014168:Transferrin / Q000378:metabolism
chemical_list: D014168:Transferrin; D005632:Fructose; D045542:PQQ Cofactor; D007501:Iron; D008354:Mannitol Dehydrogenases
keywords: E. coli Nissle 1917; Fructose; Iron; SCFA; Synbiotic