title: No correlation between MTHFR c.677 C > T, MTHFR c.1298 A > C, and ABCB1 c.3435 C > T polymorphisms and methotrexate therapeutic outcome of rheumatoid arthritis in West Algerian population.
abstract: BACKGROUND
  The c.677 C > T and c.1298 A > C polymorphisms of methylenetatrahydrofolate reductase (MTHFR) gene and c.3435 C > T polymorphism of ATP-Binding cassette B1 (ABCB1) gene are reported as pharmacogenetic markers, influencing the methotrexate (MTX) therapeutic outcome in rheumatoid arthritis (RA) patients.
  
  
  OBJECTIVE
  The aims of this study were to determine the relationship between these polymorphisms and clinical response and/or adverse drug reaction (ADRs) to MTX treatment.
  
  
  METHODS
  The cohort of our study was composed of 110 RA patients of the West Algerian population. The clinical response was evaluated using the disease activity score 28 (DAS28) and the ADRs were collected after physical examination of patients. All samples were genotyped for theses polymorphisms by TaqMan®allelic discrimination assay.
  
  
  RESULTS
  Based on EULAR criteria, 59.09% RA patients were responders and ADRs were observed in 40.9% patients. The frequency distribution of these three polymorphisms was similar between the responders and the non-responders. The same result was found on ADRs study and no significant difference of distribution between the presence of ADRs group and absence of ADRs group was observed.
  
  
  CONCLUSIONS
  Our study joins the results that found in others population in the world.
  
  
  CONCLUSIONS
  We have demonstrated, for the first time in the West Algerian population, that these polymorphisms were not predictive for clinical response and/or ADRs to MTX therapeutic outcome.
authors.affiliation: Laboratoire de génétique moléculaire et cellulaire, Université des sciences et de la technologie d'Oran Mohamed Boudiaf (USTO-MB), BP 1505, El-M'naouar, 31000, Oran, Algeria. wefaboughrara@gmail.com.|Ecole préparatoire en science de la nature et de la vie d'Oran (EPSNVO), Oran, Algeria. wefaboughrara@gmail.com.
authors.forename: Wefa
authors.initials: W
authors.lastname: Boughrara
authors.affiliation: Service de Rhumatologie, Centre Hospitalo-universitaire d'Oran (CHUO), Université d'Oran 1, Oran, Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Benzaoui
authors.affiliation: Département de pharmacie, Université d'Oran 1, Oran, Algeria.
authors.forename: Meriem
authors.initials: M
authors.lastname: Aberkane
authors.affiliation: Laboratoire de génétique moléculaire et cellulaire, Université des sciences et de la technologie d'Oran Mohamed Boudiaf (USTO-MB), BP 1505, El-M'naouar, 31000, Oran, Algeria.
authors.forename: Fatima Zohra
authors.initials: FZ
authors.lastname: Moghtit
authors.affiliation: Laboratoire de génétique moléculaire et cellulaire, Université des sciences et de la technologie d'Oran Mohamed Boudiaf (USTO-MB), BP 1505, El-M'naouar, 31000, Oran, Algeria.
authors.forename: Samia
authors.initials: S
authors.lastname: Dorgham
authors.affiliation: Laboratoire de génétique moléculaire et cellulaire, Université des sciences et de la technologie d'Oran Mohamed Boudiaf (USTO-MB), BP 1505, El-M'naouar, 31000, Oran, Algeria.
authors.forename: Aicha Sarah
authors.initials: AS
authors.lastname: Lardjam-Hetraf
authors.affiliation: Laboratoire de génétique moléculaire et cellulaire, Université des sciences et de la technologie d'Oran Mohamed Boudiaf (USTO-MB), BP 1505, El-M'naouar, 31000, Oran, Algeria.
authors.forename: Hadjer
authors.initials: H
authors.lastname: Ouhaibi-Djellouli
authors.affiliation: GenHotel-EA3886, Evry-Val d'Essonne University, Evry-Genopole, France.
authors.forename: Elisabeth Petit
authors.initials: EP
authors.lastname: Teixeira
authors.affiliation: Laboratoire de génétique moléculaire et cellulaire, Université des sciences et de la technologie d'Oran Mohamed Boudiaf (USTO-MB), BP 1505, El-M'naouar, 31000, Oran, Algeria.
authors.forename: Abdallah
authors.initials: A
authors.lastname: Boudjema
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000328:Adult; D000368:Aged; D000462:Algeria; D018501:Antirheumatic Agents / Q000009:adverse effects / Q000627:therapeutic use*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000235:genetics*; D044383:Black People / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008727:Methotrexate / Q000009:adverse effects / Q000627:therapeutic use*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D016896:Treatment Outcome
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D018501:Antirheumatic Agents; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D008727:Methotrexate
keywords: ABCB1; Algeria; MTHFR; Methotrexate; Polymorphism; Rheumatoid arthritis