title: Decompaction and recompaction of mouse preimplantation embryos.
abstract: Early (non-compacted) and late (compacted) 8-cell embryos were observed after few hours of culture in vitro. The former embryos underwent compaction and the latter embryos were found decompacted. Cell counting suggested that decompaction preceded fourth cleavage division of any blastomere and lasted until the blastomeres divided.About one third of mouse morulae, which had about twenty cells, were found non-compacted upon obtaining from females. After few hours of culture in vitro these embryos underwent recompaction and cavitation. Increasing the contributions of mitosis-arrested and cytokinesisarrested cells within the morulae by culture with nocodazole and cytochalasin B respectively, did not delay recompaction.The data show that periods of decompaction and recompaction alternate in preimplantation development.
authors.affiliation: Department of Embryology, Zoological Institute, University of Warsaw, 00-927, Warszawa, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Skrzecz
authors.affiliation: Department of Embryology, Zoological Institute, University of Warsaw, 00-927, Warszawa, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Karasiewicz
keywords: Cytochalasin B; Decompaction; Mouse preimplantation embryo; Nocodazole; Recompaction