title: Cell-Free translation of Balbiani ring RNA (75S) ofChironomus tentans salivary glands into high molecular weight products.
abstract: Cell-free translation of salivary gland RNA or of purified Balbiani ring RNA (75S) in a reticulocyte lysate system gives rise to high molecular weight translational products (HMTP). In addition to a common size (approx. 1×106daltons) HMTP share imunogenic determinants with the giant secretory proteins of salivary glands. This suggests that HMTP correspond to in vivo secreted proteins and thus, corroborates the notion that 75S-RNA is the messenger for these proteins. The time course of HMTP synthesis and the lack of appearance of lower molecular weight components as translational products of 75S-RNA indicate that the synthesis of HMTP (and of secretory proteins) occurs in one piece by an uninterrupted process. HMTP are regarded the largest polypeptides so far synthesized in a cell-free system.
authors.affiliation: Institute for Cell Biology, Swiss Federal Institute of Technology, Hönggerberg, CH-8093, Zurich, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Weber
authors.affiliation: Institute for Cell Biology, Swiss Federal Institute of Technology, Hönggerberg, CH-8093, Zurich, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Mähr
authors.affiliation: Institute for Cell Biology, Swiss Federal Institute of Technology, Hönggerberg, CH-8093, Zurich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Meyer
authors.affiliation: Institute for Cell Biology, Swiss Federal Institute of Technology, Hönggerberg, CH-8093, Zurich, Switzerland.
authors.forename: H M
authors.initials: HM
authors.lastname: Eppenberger
authors.affiliation: Institute for Cell Biology, Swiss Federal Institute of Technology, Hönggerberg, CH-8093, Zurich, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Lezzi
keywords: Balbiani ring; Cell-free translation; Chironomus messenger RNA; Secretory proteins