title: Molecular Phylogenetics ofCentrocestus formosanus(Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand.
abstract: This study aimed to investigate the morphology and reconstruct the phylogenetic relationships ofCentrocestus formosanusoriginating from 5 species of freshwater fish, i.e.,Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, andCarassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adultC. formosanusfrom day 5 after infection in chicks.C. formosanusoriginated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those fromC. auratuswhich revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability ofC. formosanusalso occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity ofC. formosanuswith 34 circumoral spines originating fromC. auratusin the future.
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.|Science and Technology Research Institute, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Chalobol
authors.initials: C
authors.lastname: Wongsawad
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Pheravut
authors.initials: P
authors.lastname: Wongsawad
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Kom
authors.initials: K
authors.lastname: Sukontason
authors.affiliation: Department of Animal and Aquatic Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Worawit
authors.initials: W
authors.lastname: Maneepitaksanti
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Nattawadee
authors.initials: N
authors.lastname: Nantarat
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes; D005618:Fresh Water; D006578:Heterophyidae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008853:Microscopy; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013785:Thailand; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV
keywords: Centrocestus formosanus; Chiang Mai; Thailand; freshwater fish; molecular phylogeny