title: NCAPH plays important roles in human colon cancer.
abstract: Colon cancer (CC) is one of the major malignancies worldwide, whose pathogenesis is complex and requires the accumulated alteration of multiple genes and signaling pathways. Condensins are multi-protein complexes that play pivotal roles in chromosome assembly and segregation during mitosis, meiosis and even tumorigenesis. Using tissue microarrays by immunohistochemistry and hematoxylin-eosin staining, we found that non-SMC condensin I complex subunit H (NCAPH) in colon cancerous tissues was higher than that in all corresponding adjacent non-cancerous tissues. We then characterized the exact function of the NCAPH in CC. We provided evidences showing that NCAPH is highly expressed in colorectal cancer cell lines comparing with normal human colonic epithelial cells, and identified many NCAPH mutations in CC patients. We found that depletion of NCAPH inhibits CC cell proliferation, migration in vitro and xenograft tumor formation in vivo. Furthermore, NCAPH knockdown promotes cell apoptosis and cell cycle arrest at G2/M phase. Interestingly, the NCAPH high expression in tumor tissues of colon patients had a significantly better prognosis and survival rate than low-expression patients, suggesting that NCAPH high expression promotes colonic cancerous cell proliferation; on the other hand, it may also sensitize these cells responding to chemo- or radio-therapies. Collectively, these findings reveal an important role of NCAPH in CC, indicating that NCAPH could be used as a new therapeutic target in future.
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming 650223, China.|The Third Affiliated Hospital of Kunming Medical University, Kunming 650223, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Jiao Chang Dong Lu 32, Kunming 650223, China.|Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Jiao Chang Dong Lu 32, Kunming 650223, China.|Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qiu-Shuo
authors.initials: QS
authors.lastname: Shen
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming 650223, China.
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Xiong
authors.affiliation: College of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhuo
authors.affiliation: College of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Long-Long
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Hai-Jing
authors.initials: HJ
authors.lastname: Yu
authors.affiliation: Faculty of Medicine, Kunming University of Science & Technology, Chenggong County, Kunming 650500, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Faculty of Medicine, Kunming University of Science & Technology, Chenggong County, Kunming 650500, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.affiliation: The Third Affiliated Hospital of Kunming Medical University, Kunming 650223, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
authors.forename: Qing-Peng
authors.initials: QP
authors.lastname: Kong
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Jiao Chang Dong Lu 32, Kunming 650223, China.
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Jiao Chang Dong Lu 32, Kunming 650223, China.
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Chen
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000502:physiology; D063646:Carcinogenesis / Q000378:metabolism / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000502:physiology; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints / Q000502:physiology; D006801:Humans; D008297:Male; D008540:Meiosis / Q000502:physiology; D008875:Middle Aged; D008938:Mitosis / Q000502:physiology; D046912:Multiprotein Complexes / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011379:Prognosis; D015996:Survival Rate
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C109974:NCAPH protein, human; D009687:Nuclear Proteins; C108312:condensin complexes; D000251:Adenosine Triphosphatases