title: Forensically Important Blow FliesChrysomya pinguis,C. villeneuvi, andLucilia porphyrina(Diptera: Calliphoridae) in a Case of Human Remains in Thailand.
abstract: This is the first study to reportChrysomya pinguis(Walker) andLucilia porphyrina(Walker) (Diptera: Calliphoridae) as forensically important blow fly species from human cadavers in Thailand, in addition toChrysomya villeneuvi(Patton) already known in Thailand. In 2016, a fully decomposed body of an unknown adult male was discovered in a high mountainous forest during winter in Chiang Mai province. The remains were infested heavily with thousands of blow fly larvae feeding simultaneously on them. Morphological identification of adults reared from the larvae, and molecular analysis based on sequencing of 1,247 bp partial mitochondrial cytochromecoxidase subunit 1 gene (CO1) of the larvae and puparia, confirmed the above mentioned 3 species. The approving forensic fly evidence by molecular approach was described for the first time in Thailand. Moreover, neighbor-joining phylogenetic analysis of theCO1was performed to compare the relatedness of the species, thereby affirming the accuracy of identification. As species of entomofauna varies among cases in different geographic and climatic circumstances,C. pinguisandL. porphyrinawere added to the list of Thai forensic entomology caseworks, including colonizers of human remains in open, high mountainous areas during winter. Further research should focus on these 3 species, for which no developmental data are currently available.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Tawatchai
authors.initials: T
authors.lastname: Monum
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Kabkaew L
authors.initials: KL
authors.lastname: Sukontason
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Pongruk
authors.initials: P
authors.lastname: Sribanditmongkol
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Kom
authors.initials: K
authors.lastname: Sukontason
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Chutharat
authors.initials: C
authors.lastname: Samerjai
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Kwankamol
authors.initials: K
authors.lastname: Limsopatham
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Suttida
authors.initials: S
authors.lastname: Suwannayod
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Tunwadee
authors.initials: T
authors.lastname: Klong-Klaew
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Wannasan
mesh_terms: D000328:Adult; D000818:Animals; D000071298:Body Remains / Q000469:parasitology*; D004175:Diptera / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D003576:Electron Transport Complex IV / Q000235:genetics; D004772:Entomology / Q000379:methods*; D044707:Forensic Sciences / Q000379:methods*; D006801:Humans; D007814:Larva / Q000145:classification / Q000235:genetics / Q000254:growth & development; D008297:Male; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D003576:Electron Transport Complex IV
keywords: Chrysomya pinguis; Chrysomya villeneuvi; Lucilia porphyrina; Thailand; forensic entomology; molecular identification