title: FBLN-4 and BCRP genes as two prognostic markers are downregulated in breast cancer tissue.
abstract: BACKGROUND
  Fibulin-4 (FBLN-4) is an extracellular glycoprotein that is upregulated in some cancer and is khown as prognostic marker in ovarian and cervical cancer. Breast cancer resistance protein (BCRP) is an ATP-binding cassette transporter that facilitates the efflux of various anticancer drugs from the cell and cause MDR phenotype in breast tumors. Many studies are available that indicat overexpression of BCRP gene in breast cancer.
  
  
  OBJECTIVE
  In the present study we aimed to analyze the expression level of FBLN-4 and BCRP in Iranian breast cancer patients.
  
  
  METHODS
  We collected 40 samples of breast cancer and normal tissue from Tehran Khatam-al-Anbia hospital. To analyze the gene expression by using Real Time RT-PCR FBLN-4 and BCRP gene expression level were measured and then the association of gene expression with breast cancer were determined.
  
  
  RESULTS
  Surprisingly the expression level of FBLN-4 and BCRP genes were downregulated in tumor tissues compared to adjacent normal tissues. Comparison of the gene expression and clinico-pathology reports indicate FBLN-4 gene expression was associated with breast cancer histological grade. We found no correlation between the expressions of BCRP gene with any clinico-pathological characters.
  
  
  CONCLUSIONS
  Interestingly and in contrast with our expectation, we found that the expression level of FBLN-4 and BCRP were downregulated in tumor compared to adjacent normal tissues. FBLN-4 was associated with grade histology and therefore can be considered as a potential prognostic biomarker.
authors.affiliation: Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Motalebzadeh
authors.affiliation: Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Frouzandeh
authors.initials: F
authors.lastname: Mahjoubi
authors.affiliation: Iran University of Medical Science, RasoleAkram Hospital, Tehran, Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Nafissi
authors.affiliation: Department of Surgery, Milad Hospital, Tehran, Iran.
authors.forename: Maria
authors.initials: M
authors.lastname: Hashemian
authors.affiliation: Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Taheri
authors.affiliation: Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Younes
authors.initials: Y
authors.lastname: Hosseinpour
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000235:genetics*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D011379:Prognosis
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; C060820:fibulin
keywords: BCRP; Fibulin-4; breast cancer; prognostic marker