title: Dual-Recognition Förster Resonance Energy Transfer Based Platform for One-Step Sensitive Detection of Pathogenic Bacteria Using Fluorescent Vancomycin-Gold Nanoclusters and Aptamer-Gold Nanoparticles.
abstract: The effective monitoring, identification, and quantification of pathogenic bacteria is essential for addressing serious public health issues. In this study, we present a universal and facile one-step strategy for sensitive and selective detection of pathogenic bacteria using a dual-molecular affinity-based Förster (fluorescence) resonance energy transfer (FRET) platform based on the recognition of bacterial cell walls by antibiotic and aptamer molecules, respectively. As a proof of concept, Vancomycin (Van) and a nucleic acid aptamer were employed in a model dual-recognition scheme for detecting Staphylococcus aureus (Staph. aureus). Within 30 min, by using Van-functionalized gold nanoclusters and aptamer-modified gold nanoparticles as the energy donor and acceptor, respectively, the FRET signal shows a linear variation with the concentration of Staph. aureus in the range from 20 to 108cfu/mL with a detection limit of 10 cfu/mL. Other nontarget bacteria showed negative results, demonstrating the good specificity of the approach. When employed to assay Staph. aureus in real samples, the dual-recognition FRET strategy showed recoveries from 99.00% to the 109.75% with relative standard derivations (RSDs) less than 4%. This establishes a universal detection platform for sensitive, specific, and simple pathogenic bacteria detection, which could have great impact in the fields of food/public safety monitoring and infectious disease diagnosis.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Mengqun
authors.initials: M
authors.lastname: Yu
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Fu
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Linyao
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Gan
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Yang
authors.identifier: 0000-0001-7716-9216
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, State University of New York , Buffalo, New York 14260, United States.
authors.forename: Mark T
authors.identifier: 0000-0002-9652-687X
authors.initials: MT
authors.lastname: Swihart
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Erqun
authors.identifier: 0000-0003-4026-090X
authors.initials: E
authors.lastname: Song
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013499:Surface Properties; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D014640:Vancomycin; D006046:Gold