title: Pomegranate Cultivars: Identification of the New IgE-Binding Protein Pommaclein and Analysis of Antioxidant Variability.
abstract: The consumption of pomegranate is increasing as it is considered a health-promoting food. Nevertheless, it can trigger allergic reactions, sometimes severe. The LTP Pun g 1 is the only pomegranate allergen so far reported. Based on preliminary clinical observations, the main aim of this study was the investigation of still unknown allergens contained in this fruit. Pommaclein, a homologue of peamaclein, the peach allergen Pru p 7, was isolated, identified by protein sequencing, and characterized as an IgE-binding protein by different test systems. RP-HPLC protein profiles revealed significant variations of LTP and pommaclein content in the red pulp of selected cultivars and accessions. Conversely, the mesocarp appeared free of proteins and much richer in antioxidants. In conclusion, a new allergen has been identified, and it could contribute to improving allergy diagnosis. The study highlights that pomegranate mesocarp could represent a rich and safe source of nutraceuticals also for allergic subjects.
authors.affiliation: Institute of Biosciences and BioResources, CNR , I-80131 Naples, Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Tuppo
authors.affiliation: Associated Centers for Molecular Allergology , Rome, Italy.|Center for Molecular Allergology, IDI-IRCCS , Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Alessandri
authors.affiliation: Fruit Trees Research Unit, CREA , I-81100 Caserta, Italy.
authors.forename: Maria Silvia
authors.initials: MS
authors.lastname: Pasquariello
authors.affiliation: Fruit Trees Research Unit, CREA , I-81100 Caserta, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Petriccione
authors.affiliation: Institute of Biosciences and BioResources, CNR , I-80131 Naples, Italy.
authors.forename: Ivana
authors.initials: I
authors.lastname: Giangrieco
authors.affiliation: Institute of Biosciences and BioResources, CNR , I-80131 Naples, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Tamburrini
authors.affiliation: Associated Centers for Molecular Allergology , Rome, Italy.|Center for Molecular Allergology, IDI-IRCCS , Rome, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Mari
authors.affiliation: Institute of Biosciences and BioResources, CNR , I-80131 Naples, Italy.
authors.forename: Maria Antonietta
authors.identifier: http://orcid.org/0000-0002-7203-0554
authors.initials: MA
authors.lastname: Ciardiello
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000975:Antioxidants / Q000032:analysis*; D005512:Food Hypersensitivity / Q000276:immunology*; D005638:Fruit / Q000737:chemistry / Q000276:immunology; D037502:Galectin 3 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D029561:Lythraceae / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment
chemical_list: D052179:Antigens, Plant; D000975:Antioxidants; D037502:Galectin 3; D010940:Plant Proteins; D007073:Immunoglobulin E
keywords: Pru p 3; Pru p 7; Pun g 1; Pun g 7; antioxidant variability; peamaclein; pomegranate; pommaclein