title: Age-related changes in skeletal muscle composition: A pilot nuclear magnetic resonance spectroscopy study in mice.
abstract: The composition of skeletal muscle was investigated in the quadriceps and gastrocnemius muscle of 13-month-old (n=15) and 23-month-old (n=19) mice by means of high-resolution nuclear magnetic resonance (NMR) spectroscopy. Muscle specimens were dissected out, frozen in liquid nitrogen and extracted in chloroform/methanol, and proton NMR spectra of the resulting aqueous and organic fractions were obtained at 600MHz. Several metabolites were unambiguously identified and quantified. Multivariate ANOVA (factor: age, muscle, age×muscle) showed a significant main effect of age (P=0.031) on the amount of muscle metabolites, suggesting that the aging process affects the composition of skeletal muscle. Univariate tests showed significant differences for lactate, acetate, taurine, and uridine in 13- and 23-month-old mice. A trend for the effect of muscle (quadriceps vs. gastrocnemius; P=0.128) was also found. No significant muscle x age interaction was present. When the same data were used in principal component analysis, the first two principal components separated muscles (quadriceps and gastrocnemius) and ages (13- and 23-month-old), explaining 66.7% of total variance. The results of this pilot study show that high-resolution NMR spectroscopy is able to detect age-associated changes in skeletal muscle metabolites, thereby paving the way to future detailed metabolomics investigation in sarcopenia of aging.
authors.affiliation: Magnetic Resonance Laboratory "Annalaura Segre", Institute of Chemical Methodologies, National Research Council, via Salaria km 29.300, I-00015 Monterotondo, Rome, Italy. Electronic address: Anatoly.sobolev@cnr.it.
authors.forename: Anatoly P
authors.initials: AP
authors.lastname: Sobolev
authors.affiliation: Magnetic Resonance Laboratory "Annalaura Segre", Institute of Chemical Methodologies, National Research Council, via Salaria km 29.300, I-00015 Monterotondo, Rome, Italy; Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, I-00185 Rome, Italy. Electronic address: Luisa.mannina@uniroma1.it.
authors.forename: Luisa
authors.initials: L
authors.lastname: Mannina
authors.affiliation: Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy. Electronic address: Manuela.costanzo@univr.it.
authors.forename: Manuela
authors.initials: M
authors.lastname: Costanzo
authors.affiliation: Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy. Electronic address: Barbara.cisterna@univr.it.
authors.forename: Barbara
authors.initials: B
authors.lastname: Cisterna
authors.affiliation: Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy. Electronic address: Manuela.malatesta@univr.it.
authors.forename: Manuela
authors.initials: M
authors.lastname: Malatesta
authors.affiliation: Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy. Electronic address: Carlo.zancanaro@univr.it.
authors.forename: Carlo
authors.initials: C
authors.lastname: Zancanaro
mesh_terms: D000085:Acetates / Q000032:analysis; D000375:Aging / Q000502:physiology*; D000818:Animals; D019344:Lactic Acid / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010865:Pilot Projects; D025341:Principal Component Analysis; D052097:Quadriceps Muscle / Q000378:metabolism*; D013654:Taurine / Q000032:analysis; D014529:Uridine / Q000032:analysis
chemical_list: D000085:Acetates; D013654:Taurine; D019344:Lactic Acid; D014529:Uridine
keywords: Aging; Gastrocnemius muscle; Nuclear magnetic resonance spectroscopy; Quadriceps muscle