title: A Light-Addressable Potentiometric Sensor for Odorant Detection Using Single Bioengineered Olfactory Sensory Neurons as Sensing Element.
abstract: A light-addressable potentiometric sensor (LAPS), a silicon-based surface potential detector, is combined with bioengineered olfactory sensory neurons (OSN) for odorant detection. A LAPS chip is used as a transducer to monitor cell membrane potential changes. In addition, a focused movable laser with a diameter comparable to cell sizes is employed to select the desirable single cell for measurement under a microscope. Bioengineered OSNs are coupled to the LAPS surface and employed as sensing elements, which are prepared by the expression of an olfactory receptor of C. elegans, ODR-10, on the plasma membrane of rat primary OSNs via transient transfection. The responses of bioengineered OSNs to diacetyl, isoamyl acetate, and acetic acid are monitored by extracellular recording using the LAPS chip. Features of the recorded extracellular potential firings are analyzed in frequency and time domains. We have shown that bioengineered OSNs can generate specific response signals upon the stimulation of diacetyl, which is the natural ligand of ODR-10. Moreover, different concentrations of diacetyl can elicit different temporal firing patterns in bioengineered OSNs, which permits the concentration detection of specific odorant molecules in solution.
authors.affiliation: Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wu
authors.affiliation: Institute of Medical Engineering, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Du
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China. cnpwang@zju.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017173:Caenorhabditis elegans; D018929:Cell Culture Techniques; D002469:Cell Separation; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D008027:Light; D008564:Membrane Potentials; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000502:physiology* / Q000528:radiation effects*; D051381:Rats; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism; D013223:Statistics as Topic / Q000379:methods
chemical_list: D018035:Receptors, Odorant
keywords: Bioengineered cell; Biosensor; Light-addressable potentiometric sensor; Odorant; Olfactory sensory neuron