title: Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant.
abstract: The anaerobic protozoan parasite Entamoeba histolytica has mitosomes that are mitochondria lacking some canonical functions and organelle DNA. Mitosomes play an important role in the life cycle of the parasite. The distribution of proteins in mitosomes is not uniform, and how mitosomes are maintained and retained is unknown. To answer these questions, we developed a transplant method for mitosomes with hemagglutinin-tagged protein into recipient cells containing mitosomes with Myc-tagged protein. Immunofluorescence staining showed that the two protein tags colocalized in single mitosomes in some recipient cells. These results suggest that our transplant method can be used in anaerobic protozoa and that donor mitosomes may obtain recipient proteins through fusion with other mitosomes or through de novo synthesis of proteins in recipient cells.
authors.affiliation: Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kazama
authors.affiliation: Support Center for Medical Research and Education, Tokai University, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Sanae
authors.initials: S
authors.lastname: Ogiwara
authors.affiliation: Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Makiuchi
authors.affiliation: Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Nakada-Tsukui
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.|Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8577, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Nozaki
authors.affiliation: Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tachibana
mesh_terms: D000693:Anaerobiosis; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000235:genetics / Q000378:metabolism*; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism* / Q000637:transplantation; D063154:Mitochondrial Dynamics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D053058:Trophozoites / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D004272:DNA, Mitochondrial; D006388:Hemagglutinins; D016271:Proto-Oncogene Proteins c-myc; D015800:Protozoan Proteins