title: Response of alternative splice isoforms of OsRad9 gene from Oryza sativa to environmental stress.
abstract: Rad9 protein plays an important role in cell-cycle checkpoint signal transduction in human and yeast cells, but knowledge about Rad9 in plants is limited. This study reports that the Rad9 gene of rice can generate the transcript products OsRad9.1 and OsRad9.2 through alternative splicing. OsRad9.1, with all nine exons, is the main cell-cycle checkpoint protein involved in the response of rice to genotoxic stresses (ultraviolet radiation and antibiotic stress), environmental stresses (drought, salt, and heavy metal stress), and auxin stimuli (2,4-D, IAA, and IBA). Meanwhile, transcript isoform OsRad9.2, which lost exon7 and exon8, showed different preferential stimulation effects on these stresses and pollen development duration. These results might indicat that besides the monitoring and repair of DNA damage, Rad9 might involve in the development of pollen.
authors.affiliation: College of Pharmacy and Biological Engineering, Chengdu University, 1 Shiling, Chengdu 610106, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, China.
authors.forename: Wenguo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Lab of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Fosheng
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Lab of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Lab of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Lab of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China, Tel.: +86 28 85417281, Fax: 86 028 85417281.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Lab of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D055864:Droughts; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D007210:Indoleacetic Acids / Q000494:pharmacology; D019216:Metals, Heavy / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014466:Ultraviolet Rays
chemical_list: D018797:Cell Cycle Proteins; D007210:Indoleacetic Acids; D019216:Metals, Heavy; D010937:Plant Growth Regulators; D010940:Plant Proteins; C069930:rad9 protein; D012965:Sodium Chloride
keywords: Rad9; abiotic stress; alternative splicing; auxin; gene expression