title: Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS.
abstract: Deletion of the biosynthetic 4,6-dehydratase gene, jadT, present in the angucycline jadomycin dideoxysugar biosynthetic pathway, led to the isolation of a novel C12 glucosylated jadomycin. JadS was identified as the catalyst responsible for glucosylation due to a loss of production of the glucosylated natural product in a ΔjadSΔjadT deletion strain. This study demonstrates that a 2,6-dideoxy-l-sugar glycosyltransferase is able to transfer d-glucose, exemplifying remarkable substrate tolerance.
authors.affiliation: Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada. David.Jakeman@dal.ca.
authors.forename: S M
authors.initials: SM
authors.lastname: Forget
authors.affiliation: Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada. David.Jakeman@dal.ca.
authors.forename: Jungwook
authors.initials: J
authors.lastname: Na
authors.affiliation: College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: N E
authors.initials: NE
authors.lastname: McCormick
authors.affiliation: Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada. David.Jakeman@dal.ca and College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: D L
authors.identifier: http://orcid.org/0000-0003-3002-3388
authors.initials: DL
authors.lastname: Jakeman
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D017353:Gene Deletion; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D006836:Hydro-Lyases / Q000235:genetics* / Q000378:metabolism; D007546:Isoquinolines / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008968:Molecular Conformation; D013379:Substrate Specificity
chemical_list: D001688:Biological Products; D007546:Isoquinolines; C587310:jadomycin S; D016695:Glycosyltransferases; D006836:Hydro-Lyases