title: Microsomal glutathione transferase 1 attenuated ROS-induced lipid peroxidation in Apostichopus japonicus.
abstract: Microsomal glutathione transferase (mGST) is a membrane bound glutathione transferase in multifunctional detoxification isoenzymes family and also plays crucial roles in innate immunity. In the present study, a novel microsomal GST homology was identified from Apostichopus japonicus (designated as AjmGST1) by RACE approaches. The full-length cDNA of AjmGST1 was of 1296 bp encoded a protein of 169 amino acids residues. Multiple sequence alignment and phylogenetic analysis together supported that AjmGST1 belonged to a new member in invertebrates mGST family. Spatial expression analysis revealed that AjmGST1was ubiquitously expressed in all examined tissues with the larger magnitude in tentacle. Time-course expression of AjmGST1 mRNA in coelomocytes was up-regulated after Vibrio splendidus challenge from 6 h until 72 h with the peak expression in 24 h, compared with that in the control group. Similarly, the induced expression of AjmGST1 expression was also detected in lipopolysaccharide (LPS) exposed primary coelomocytes. The purified recombinant protein of AjmGST1 showed high activity with GST substrate at pH of 7.0 and temperature of 35 °C. Meantime, the recombinant AjmGST1 depressed H2O2-induced MDA production both in vivo and in vitro. All of these results indicated that AjmGST1 was an important regulator in elimination of lipid peroxidation under immune response.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Zhimeng
authors.initials: Z
authors.lastname: Lv
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Yina
authors.initials: Y
authors.lastname: Shao
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China. Electronic address: qiuqiongfen@nbu.edu.cn.
authors.forename: Qiongfen
authors.initials: Q
authors.lastname: Qiu
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Duan
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D005982:Glutathione Transferase / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D015227:Lipid Peroxidation / Q000276:immunology; D017382:Reactive Oxygen Species / Q000276:immunology; D047528:Stichopus / Q000276:immunology*
chemical_list: D017382:Reactive Oxygen Species; D005982:Glutathione Transferase
keywords: Apostichopus japonicus; Gene expression; Immune response; MDA; Microsomal glutathione S-Transferase