title: Biocatalysis engineering: the big picture.
abstract: In this tutorial review we describe a holistic approach to the invention, development and optimisation of biotransformations utilising isolated enzymes. Increasing attention to applied biocatalysis is motivated by its numerous economic and environmental benefits. Biocatalysis engineering concerns the development of enzymatic systems as a whole, which entails engineering its different components: substrate engineering, medium engineering, protein (enzyme) engineering, biocatalyst (formulation) engineering, biocatalytic cascade engineering and reactor engineering.
authors.affiliation: Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Johannesburg 2050, South Africa. roger.sheldon@wits.ac.za.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Sheldon
authors.forename: Pedro C
authors.initials: PC
authors.lastname: Pereira
mesh_terms: D000818:Animals; D055162:Biocatalysis; D004798:Enzymes / Q000378:metabolism*; D015202:Protein Engineering
chemical_list: D004798:Enzymes