title: Transcriptomic analysis of basidiocarp development in Ustilago maydis (DC) Cda.
abstract: Previously, we demonstrated that when Ustilago maydis (DC) Cda., a phytopathogenic basidiomycete and the causal agent of corn smut, is grown in the vicinity of maize embryogenic calli in a medium supplemented with the herbicide Dicamba, it developed gastroid-like basidiocarps. To elucidate the molecular mechanisms involved in the basidiocarp development by the fungus, we proceeded to analyze the transcriptome of the process, identifying a total of 2002 and 1064 differentially expressed genes at two developmental stages, young and mature basidiocarps, respectively. Function of these genes was analyzed with the use of different databases. MIPS analysis revealed that in the stage of young basidiocarp, among the ca. two thousand differentially expressed genes, there were some previously described for basidiocarp development in other fungal species. Additional elements that operated at this stage included, among others, genes encoding the transcription factors FOXO3, MIG3, PRO1, TEC1, copper and MFS transporters, and cytochromes P450. During mature basidiocarp development, important up-regulated genes included those encoding hydrophobins, laccases, and ferric reductase (FRE/NOX). The demonstration that a mapkk mutant was unable to form basidiocarps, indicated the importance of the MAPK signaling pathway in this developmental process.
authors.affiliation: Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico.
authors.forename: C G
authors.initials: CG
authors.lastname: León-Ramírez
authors.affiliation: Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico. Electronic address: jlcabre@yahoo.com.mx.
authors.forename: J L
authors.initials: JL
authors.lastname: Cabrera-Ponce
authors.affiliation: Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico.
authors.forename: D
authors.initials: D
authors.lastname: Martínez-Soto
authors.affiliation: Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico; Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolas de los Garza, Nuevo León, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Sánchez-Arreguin
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolas de los Garza, Nuevo León, Mexico.
authors.forename: E T
authors.initials: ET
authors.lastname: Aréchiga-Carvajal
authors.affiliation: Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico. Electronic address: jruiz@ira.cinvestav.mx.
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Herrera
mesh_terms: D003996:Dicamba / Q000494:pharmacology*; D048690:Fruiting Bodies, Fungal / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000096:biosynthesis; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology; D059467:Transcriptome / Q000187:drug effects*; D014588:Ustilago / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D003313:Zea mays / Q000382:microbiology
chemical_list: D005656:Fungal Proteins; D003996:Dicamba
keywords: Basidiocarps; Copper transport; Dicamba; MAPK pathway; Transcription factors; Ustilago maydis