title: Distribution ofcfrinStaphylococcusspp. andEscherichia coliStrains from Pig Farms in China and Characterization of a Novelcfr-Carrying F43:A-:B- Plasmid.
abstract: The multi-resistance genecfris widely distributed among various gram-positive and gram-negative species in livestock in China. To better understand the epidemiology ofcframongStaphylococcusspp. andE. coliisolates, 254Staphylococcusspp. and 398E. colistrains collected from six swine farms in China were subjected to prevalence and genetic analysis. Forty (15.7%)Staphylococcusspp. isolates, including 38Staphylococcus sciuristrains, oneStaphylococcus chromogenesstrain, and oneStaphylococcus lentusstrain, and two (0.5%)E. coliisolates were found to contain thecfrgene. Most of the 38S. sciuristrains were clonally unrelated; however, clonal dissemination ofcfr-positiveS. sciuriwas detected at the same farm. In eight randomly selectedcfr-positive staphylococci, acfr-harboring module (IS21-558-cfr-ΔtnpB) was detected in sixS. sciuriisolates;cfrwas bracketed by two copies of ISEnfa4or IS256in the remaining twoS. sciuriisolates. In the twoE. coliisolates, EP25 and EP28,cfrwas flanked by two IS26elements in the same or opposite orientation, respectively. Complete sequence analysis of the novel F43:A-:B- plasmid pHNEP28 revealed that it contains two multi-resistance regions:cfrtogether withfloR,qnrS1interspersed with IS26, ΔISCR2and ISKpn19, andblaTEM-1together withtet(M) interspersed with IS26, ISApl1, ΔTn2, and ΔIS1B. The coexistence ofcfrwith other resistance genes on a conjugative plasmid may contribute to the dissemination of these genes by co-selection. Thus, rational drug use and continued surveillance ofcfrin swine farms are warranted.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Zhao
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Liu
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Zhen-Ling
authors.initials: ZL
authors.lastname: Zeng
keywords: Escherichia coli; F43:A-:B- plasmid; Staphylococcus spp.; cfr; swine