title: Design and Development of Acetylthiocholine Electrochemical Biosensor Based on Zinc Oxide-Cerium Oxide Nanohybrid Modified Platinum Electrode.
abstract: Acetylcholinesterase (AChE) enzyme has been predominantly used for the detection of pesticides and metal ions. But, these sensors respond to pesticides as well as metal ions at certain concentration, which results in poor selectivity. Hence in this work, the amount of thiocholine produced during AChE inhibition has been estimated to detect the residual activity of AChE enzyme in-turn to enhance the efficiency of the biosensor. In this context, Pt/ZnO-CeO2/AChE/Chitosan based biosensor has been developed for sensitive voltammetric quantification of thiocholine in AChE. The sensor exhibited enhanced electron transfer rate, good conductivity and biocompatibility. Both the intrinsic and extrinsic parameters were simultaneously optimized using second order polynomial regression to get the best conditions for ATCh determination. Under optimized experimental conditions, the redox peak current was linear over the concentration range of 0.1-1.5 mM with detection and quantification limit of 0.05 and 0.15 μM respectively and the sensitivity of 1.47 μA mM-1.
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Manju Bhargavi
authors.initials: MB
authors.lastname: Gumpu
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Noel
authors.initials: N
authors.lastname: Nesakumar
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Srinidhi
authors.initials: S
authors.lastname: Nagarajan
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Sadhana
authors.initials: S
authors.lastname: Ramanujam
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: K Jayanth
authors.initials: KJ
authors.lastname: Babu
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India. rjbosco@ece.sastra.edu.|School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India. rjbosco@ece.sastra.edu.|Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India. rjbosco@ece.sastra.edu.
authors.forename: John Bosco Balaguru
authors.initials: JB
authors.lastname: Rayappan
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000122:Acetylthiocholine / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D002563:Cerium / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000378:metabolism; D004566:Electrodes; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis; D010984:Platinum / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013860:Thiocholine / Q000032:analysis; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D002800:Cholinesterase Inhibitors; D010575:Pesticides; D002563:Cerium; D000122:Acetylthiocholine; D010984:Platinum; C030583:ceric oxide; D013860:Thiocholine; D048271:Chitosan; D000110:Acetylcholinesterase; D015034:Zinc Oxide
keywords: Acetylcholinesterase; Acetylthiocholine; Cyclic voltammetry; Electrochemical biosensor; Multivariate optimization; Nanohybrid