title: Methotrexate causes acute hyperplasia of enterochromaffin cells containing substance P in the intestinal mucosa of rats.
abstract: This study aimed to investigate the acute and chronic effect of methotrexate on the intestinal substance P metabolism after a single administration to rats. Methotrexate caused a significant increase in the number of substance P-containing cells in the ileal mucosa both at 24 and 96 h. Most of enterochromaffin cells expressing l-tryptophan hydroxylase contained substance P. The expression of Tac1 mRNA was increased by methotrexate at 24 h, but not at 96 h. Thus, methotrexate causes acute hyperplasia of enterochromaffin cells in the intestinal mucosa of rats with a transient increase in the production of substance P.
authors.affiliation: Department of Pharmacological Sciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Machida
authors.affiliation: Department of Pharmacological Sciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Yuho
authors.initials: Y
authors.lastname: Takano
authors.affiliation: Department of Pharmacological Sciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Iizuka
authors.affiliation: Division of Pharmacotherapy, Hokkaido Pharmaceutical University School of Pharmacy, Sapporo, Hokkaido 006-8590, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Machida
authors.affiliation: Department of Pharmacological Sciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan. Electronic address: hirafuji@hoku-iryo-u.ac.jp.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hirafuji
mesh_terms: D000818:Animals; D004759:Enterochromaffin Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006965:Hyperplasia / Q000139:chemically induced* / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D013373:Substance P / Q000378:metabolism*; D015320:Tachykinins / Q000235:genetics
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C054618:protachykinin; D013373:Substance P; D008727:Methotrexate
keywords: Enterochromaffin cells; Methotrexate; Substance P