title: Prevalence of adeno-associated virus and human papillomavirus DNA in Iranian women with and without cervical cancer.
abstract: There is plenty of substantial evidence to support anti-tumor activity of viruses. Adeno-associated virus (AAV) may interact with human papillomavirus (HPV) to modify the risk of cervical neoplasia. The seroprevalence of AAV among women with cervical cancer has been reported to be lower than healthy ones. In spite of this finding, detection of AAV DNA in cervical biopsies does not entirely support the inverse association between AAV seropositivity and cervical cancer. This association is still controversial and requires more thorough evaluation in different countries. The aim of this case-control study was to find the prevalence of AAV and HPV DNA sequences in Iranian women with and without cervical cancer to assess the probable association of AAV infection and cervical cancer. In this study, paraffin-embedded tissue samples of 61 cervical cancer cases and 50 healthy controls (HCs) were investigated for AAV and HPV DNA by semi-nested and nested PCRs respectively. AAV DNA was detected in 7 cases (14%) of HCs and 9 specimens (14.8%) of case group. According to the branching in the phylogenetic tree, AAV2 was the only type detected in this study. Moreover, HPV DNA was detected in 8 cases (16%) of HCs and 44 specimens (72.13%) of case group. In conclusion, a low proportion of cervical biopsies from Iranian women contained AAV-2 genome. No significant difference in correlation between HPV and cervical cancer in presence or absence of AAV genome in cervix was found.
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nazanin Zahra
authors.initials: NZ
authors.lastname: Shafiei-Jandaghi
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Jila
authors.initials: J
authors.lastname: Yavarian
authors.affiliation: Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Faghihloo
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Ghavami
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Zohreh Yousefi
authors.initials: ZY
authors.lastname: Ghalejoogh
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Jalal
authors.initials: SJ
authors.lastname: Kiani
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Somayeh Shatizadeh
authors.initials: SS
authors.lastname: Malekshahi
authors.affiliation: Pathology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Shahsiah
authors.affiliation: Pathology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Eisa
authors.initials: E
authors.lastname: Jahanzad
authors.affiliation: Epidemiology and Biostatistics Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: mokhtari@tums.ac.ir.
authors.forename: Talat Mokhtari
authors.initials: TM
authors.lastname: Azad
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics; D000229:Dependovirus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D016612:Paraffin Embedding; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000821:virology
chemical_list: D004279:DNA, Viral
keywords: AAV; Cervical cancer; HPV; Iran