title: The renoprotective effect of oral Tadalafil pretreatment on ischemia/reperfusion injury in rats.
abstract: To evaluate the effect of tadalafil in renal ischemia/reperfusion (I/R) injury in rats Methods: Group I/R saline rats (n=6) were subjected to 45 minutes of left renal ischemia and treated with saline; the I/R tadalafil rats (n=6) received oral 10mg/kg tadalafil microemulsion one hour before ischemia. In both groups, 8 hours after ischemia, laboratory analysis were performed Results: Better tissue perfusion was lower in ischemic left/kidney than in right/kidney in saline group, suggesting reduced kidney clearance. Fluorescence in left/kidneys of tadalafil treated rats was lower than in right/kidneys (difference not significant). The fluorescence signal intensity in kidneys of tadafil treated rats was higher than in saline rats. TNF-α levels were significantly lower in I/R tadalafil group rats compared to I/R saline group (154±10.3 vs 391.3±12.3), as well as IL-1β (163.4±13.2 vs 279±11.5pg/dL), and IL-6 (122.9±8.1 vs 173.7±6.3 respectively; p=0.0001). Urea, creatinine and C-reactive protein were significantly lower in tadafil treated rats then in saline group Conclusion: Tadalafil therapy decreased the expression of circulating pro-inflammatory cytokines in a renal I/R rodent model, while improving kidney function proofs.
authors.affiliation: Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Vanessa de Fátima Lima Paiva
authors.initials: VF
authors.lastname: Medeiros
authors.affiliation: Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Ítalo Medeiros
authors.initials: ÍM
authors.lastname: Azevedo
authors.affiliation: Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Marília Daniela Ferreira
authors.initials: MD
authors.lastname: Carvalho
authors.affiliation: Postgraduate Program in Health Sciences, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Cláudia Nunes
authors.initials: CN
authors.lastname: Oliveira
authors.affiliation: Department of Pharmacy, UFRN, Natal, RN, Brazil.
authors.forename: Eryvaldo Sócrates Tabosa do
authors.initials: ES
authors.lastname: Egito
authors.affiliation: Nucleus of Experimental Surgery, UFRN, Natal, RN, Brazil.
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D016207:Cytokines; D005453:Fluorescence; D007668:Kidney / Q000187:drug effects* / Q000293:injuries; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000517:prevention & control*; D000068581:Tadalafil / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D014665:Vasodilator Agents; D000068581:Tadalafil