title: Differential role for PAK1 and PAK4 during the invadopodia lifecycle.
abstract: PAK1 and PAK4 are members of the p-21 activated kinase family of serine/threonine kinases. PAK1 has previously been implicated in both the formation and disassembly of invasive cell protrusions, termed invadopodia. We recently reported a novel role for PAK4 during invadopodia maturation and confirmed a specific role for PAK1 in invadopodia formation; findings we will review here. Moreover, we found that PAK4 induction of maturation is delivered via interaction with the RhoA regulator PDZ-RhoGEF. We can now reveal that loss of PAK4 expression leads to changes in invadopodia dynamics. Ultimately we propose that PAK4 but not PAK1 is a key mediator of RhoA activity and provide further evidence that modulation of PAK4 expression levels leads to changes in RhoA activity.
authors.affiliation: a Division of Cancer Studies , New Hunts House, Guy's Campus, King's College London , London , UK.|b National Institute for Health Research (NIHR) Biomedical Research Centre, Guy's and St Thomas's Hospital and King's College London , London , UK.
authors.forename: Nicole S
authors.initials: NS
authors.lastname: Nicholas
authors.affiliation: a Division of Cancer Studies , New Hunts House, Guy's Campus, King's College London , London , UK.|b National Institute for Health Research (NIHR) Biomedical Research Centre, Guy's and St Thomas's Hospital and King's College London , London , UK.
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Pipili
authors.affiliation: a Division of Cancer Studies , New Hunts House, Guy's Campus, King's College London , London , UK.
authors.forename: Michaela S
authors.initials: MS
authors.lastname: Lesjak
authors.affiliation: a Division of Cancer Studies , New Hunts House, Guy's Campus, King's College London , London , UK.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Wells
mesh_terms: D002460:Cell Line; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D000069261:Podosomes / Q000378:metabolism*; D064067:Rho Guanine Nucleotide Exchange Factors / Q000378:metabolism; D054462:p21-Activated Kinases / Q000235:genetics / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: C577234:ARHGEF11 protein, human; D064067:Rho Guanine Nucleotide Exchange Factors; C066737:RHOA protein, human; C116367:PAK4 protein, human; C515903:PAK1 protein, human; D054462:p21-Activated Kinases; D020742:rhoA GTP-Binding Protein
keywords: PAK; RhoA; invadopodia