title: The application of capillary microsampling in GLP toxicology studies.
abstract: OBJECTIVE
  Capillary microsampling (CMS) to collect microplasma volumes is gradually replacing traditional, larger volume sampling from rats in GLP toxicology studies.
  
  
  METHODS
  About 32 µl of blood is collected with a capillary, processed to plasma and stored in a 10- or 4-µl capillary which is washed out further downstream in the laboratory. CMS has been standardized with respect to materials, assay validation experiments and application for sample analysis.
  
  
  CONCLUSIONS
  The implementation of CMS has resulted in blood volume reductions in the rat from 300 to 32 µl per time point and the elimination of toxicokinetic satellite groups in the majority of the rat GLP toxicology studies. The technique has been successfully applied in 26 GLP studies for 12 different projects thus far.
authors.affiliation: Development Bioanalysis, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Verhaeghe
authors.affiliation: Development Bioanalysis, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Lieve
authors.initials: L
authors.lastname: Dillen
authors.affiliation: Development Bioanalysis, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Hans
authors.initials: H
authors.lastname: Stieltjes
authors.affiliation: Pharmacokinetics, Dynamics & Metabolism, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Loeckie de
authors.initials: L
authors.lastname: Zwart
authors.affiliation: Preclinical Development & Safety, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Bianca
authors.initials: B
authors.lastname: Feyen
authors.affiliation: Development Bioanalysis, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Diels
authors.affiliation: Development Bioanalysis, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Ann
authors.initials: A
authors.lastname: Vroman
authors.affiliation: Development Bioanalysis, Janssen Research & Development, Division of Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Philip
authors.initials: P
authors.lastname: Timmerman
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D002196:Capillaries; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D064420:Drug-Related Side Effects and Adverse Reactions; D007753:Laboratories / Q000592:standards*; D004364:Pharmaceutical Preparations / Q000097:blood*; D051381:Rats; D014116:Toxicology / Q000592:standards*
chemical_list: D004364:Pharmaceutical Preparations
keywords: GLP; NC3Rs; assay validation; capillary microsampling; end-to-end capillaries; rat studies; toxicokinetics; toxicology