title: Differential Gene Expression of Longan Under Simulated Acid Rain Stress.
abstract: Differential gene expression profile was studied in Dimocarpus longan Lour. in response to treatments of simulated acid rain with pH 2.5, 3.5, and a control (pH 5.6) using differential display reverse transcription polymerase chain reaction (DDRT-PCR). Results showed that mRNA differential display conditions were optimized to find an expressed sequence tag (EST) related with acid rain stress. The potential encoding products had 80% similarity with a transcription initiation factor IIF of Gossypium raimondii and 81% similarity with a protein product of Theobroma cacao. This fragment is the transcription factor activated by second messenger substances in longan leaves after signal perception of acid rain.
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zheng
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Pan
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Cuilan
authors.initials: C
authors.lastname: Ma
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. qiudl1970@fafu.edu.cn.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Qiu
mesh_terms: D015258:Acid Rain; D000595:Amino Acid Sequence; D001483:Base Sequence; D020869:Gene Expression Profiling; D018515:Plant Leaves / Q000235:genetics*; D018749:RNA, Plant / Q000302:isolation & purification; D029631:Sapindaceae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D015258:Acid Rain; D018749:RNA, Plant
keywords: DDRT-PCR; Dimocarpus longan Lour.; EST