title: New platelet functional method for identification of pathogenic antibodies in HIT patients.
abstract: Heparin-induced thrombocytopenia (HIT) is a thrombotic complication of heparin therapy. The most used functional method for HIT diagnosis is serotonin release assay (SRA). A different functional method based on ATP release with luciferin/luciferase long-life and stable luminescent signal is used here, which is shown to be comparable for accuracy with SRA in both negative (patients 4Ts ≤3, and negative for both anti-PF4/heparin immunoassay and SRA) and positive (4Ts >3, and positive for both PF4/heparin antibodies and SRA) patients. Our results show that ATP release is higher in washed platelets activated by sera from positive patients than in platelets activated by sera from negative patients. In conclusion, we demonstrate that ATP release assay is a valid alternative method to SRA for the identification of pathogenic anti-PF4/heparin antibodies.
authors.affiliation: a Department of Experimental Medicine , Sapienza University of Rome , Rome , Italy.
authors.forename: M L
authors.initials: ML
authors.lastname: Guarino
authors.affiliation: a Department of Experimental Medicine , Sapienza University of Rome , Rome , Italy.
authors.forename: I
authors.initials: I
authors.lastname: Massimi
authors.affiliation: a Department of Experimental Medicine , Sapienza University of Rome , Rome , Italy.
authors.forename: S
authors.initials: S
authors.lastname: Mardente
authors.affiliation: b Cardiovascular and Anesthesia-Intensive Care and Department of Cardiac and Heart Transplantation San Camillo-Forlanini Hospital , Rome , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Lappa
authors.affiliation: b Cardiovascular and Anesthesia-Intensive Care and Department of Cardiac and Heart Transplantation San Camillo-Forlanini Hospital , Rome , Italy.
authors.forename: S
authors.initials: S
authors.lastname: Donfrancesco
authors.affiliation: c T2 Biosystems, Inc ., Lexington , MA , USA.
authors.forename: G P
authors.initials: GP
authors.lastname: Visentin
authors.affiliation: a Department of Experimental Medicine , Sapienza University of Rome , Rome , Italy.
authors.forename: F M
authors.initials: FM
authors.lastname: Pulcinelli
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood*; D000925:Anticoagulants / Q000009:adverse effects* / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology / Q000473:pathology; D005260:Female; D049454:Firefly Luciferin / Q000737:chemistry; D005500:Follow-Up Studies; D015870:Gene Expression; D006493:Heparin / Q000009:adverse effects* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D049409:Luciferases, Firefly / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010978:Platelet Factor 4 / Q000235:genetics / Q000276:immunology; D012701:Serotonin / Q000097:blood*; D013921:Thrombocytopenia / Q000097:blood / Q000139:chemically induced / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000906:Antibodies; D000925:Anticoagulants; C485197:luciferase, Photinus; D012701:Serotonin; D010978:Platelet Factor 4; D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D006493:Heparin; D049409:Luciferases, Firefly
keywords: ATP release; HIT