title: Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier.
abstract: The controlled delivery of drug/imaging agents to cells is critical for the development of therapeutics and for the study of cellular signaling processes. Recently, nanoparticles (NPs) have shown significant promise in the development of such delivery systems. Here, a liquid crystal NP (LCNP)-based delivery system has been employed for the controlled delivery of a water-insoluble dye, 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO), from within the NP core to the hydrophobic region of a plasma membrane bilayer. During the synthesis of the NPs, the dye was efficiently incorporated into the hydrophobic LCNP core, as confirmed by multiple spectroscopic analyses. Conjugation of a PEGylated cholesterol derivative to the NP surface (DiO-LCNP-PEG-Chol) enabled the binding of the dye-loaded NPs to the plasma membrane in HEK 293T/17 cells. Time-resolved laser scanning confocal microscopy and Förster resonance energy transfer (FRET) imaging confirmed the passive efflux of DiO from the LCNP core and its insertion into the plasma membrane bilayer. Finally, the delivery of DiO as a LCNP-PEG-Chol attenuated the cytotoxicity of DiO; the NP form of DiO exhibited ~30-40% less toxicity compared to DiOfree delivered from bulk solution. This approach demonstrates the utility of the LCNP platform as an efficient modality for the membrane-specific delivery and modulation of hydrophobic molecular cargos.
authors.affiliation: Center for Biomolecular Science and Engineering, Naval Research Laboratory.
authors.forename: Okhil K
authors.initials: OK
authors.lastname: Nag
authors.affiliation: Center for Biomolecular Science and Engineering, Naval Research Laboratory.
authors.forename: Jawad
authors.initials: J
authors.lastname: Naciri
authors.affiliation: Optical Sciences Division, Naval Research Laboratory; Sotera Defense Solutions.
authors.forename: Eunkeu
authors.initials: E
authors.lastname: Oh
authors.affiliation: Center for Biomolecular Science and Engineering, Naval Research Laboratory.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Spillmann
authors.affiliation: Center for Biomolecular Science and Engineering, Naval Research Laboratory; james.delehanty@nrl.navy.mil.
authors.forename: James B
authors.initials: JB
authors.lastname: Delehanty
mesh_terms: D002462:Cell Membrane; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000295:instrumentation*; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D050866:Liquid Crystals; D053758:Nanoparticles / Q000737:chemistry*