title: Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase.
abstract: Although aerobic CO dehydrogenases (CODHs) might be applicable in various fields, their practical applications have been hampered by low activity and no heterologous expression. We, for the first time, could functionally express recombinant PsCODH in E. coli and obtained a highly concentrated recombinant enzyme using an easy and convenient method. Its electron acceptor spectra, optimum conditions (pH 6.5 and 30 °C), and kinetic parameters (kcatof 12.97 s-1, Kmof 0.065 mM, and specific activity of 0.86 Umg-1) were examined. Blast furnace gas (BFG) containing 20% CO, which is a waste gas from the steel-making process, was tested as a substrate for PsCODH. Even with BFG, the recombinant PsCODH retained 88.2% and 108.4% activity compared with those of pure CO and 20% CO, respectively. The results provide not only a promising strategy to utilize CO-containing industrial waste gases as cheap, abundant, and renewable resources but also significant information for further studies about cascade reactions producing value-added chemicals via CO2as an intermediate produced by a CODH-based CO-utilization system, which would ultimately expand the versatility of CODH.
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.|Department of Biological Science, College of Natural Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Eun Sil
authors.initials: ES
authors.lastname: Choi
authors.affiliation: School of Energy and Chemical Engineering, UNIST, Ulsan 44919, Republic of Korea.
authors.forename: Kyoungseon
authors.initials: K
authors.lastname: Min
authors.affiliation: Department of Biological Science, College of Natural Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Geun-Joong
authors.initials: GJ
authors.lastname: Kim
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
authors.forename: Inchan
authors.initials: I
authors.lastname: Kwon
authors.affiliation: School of Energy and Chemical Engineering, UNIST, Ulsan 44919, Republic of Korea.
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis*; D002245:Carbon Dioxide / Q000737:chemistry* / Q000378:metabolism; D002248:Carbon Monoxide / Q000737:chemistry* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis; D007700:Kinetics; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D020636:Pantoea / Q000737:chemistry* / Q000145:classification / Q000201:enzymology; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D056804:Biofuels; D007220:Industrial Waste; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D002248:Carbon Monoxide; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase