title: Tissue-specific daily variation in the oxidative status of sturgeon (Acipenser naccarii) and rainbow trout (Oncorhynchus mykiss): a comparative study.
abstract: The oxidative status is associated with animal lifespan, metabolism, activity and circadian rhythms. The objective of this work is to study the time course of the oxidative status over a daily cycle in the plasma, liver and brain, and the changes in the plasma cortisol levels of sturgeon and trout. The knowledge of daily oxidative status will provide a better understanding of the trout and sturgeon physiology and adequate maintenance and food supply of farmed fish in relation to photoperiod. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase, DT-diaphorase activities, lipid peroxidation and cortisol were measured. Our results showed that the antioxidative enzyme activities and lipid peroxidation in the liver of trout and sturgeon changed through the day, with increased levels in lipid peroxidation of liver in the dark period for sturgeon. This could be related to the different activity time in both species, an issue to be taken into account when designing the guidelines of the maintenance of these species in fish farms. On the contrary, there was not clear influence of the daily rhythms on brain oxidative status. The higher efficiency of the antioxidant defences in the brain of sturgeon, which displays less lipid peroxidation and higher antioxidative activity, could be related to its longer life expectancy. The absence of any apparent daily rhythm in the plasma cortisol levels in sturgeon could indicate a lower susceptibility to stress, and that mechanisms involved in cortisol secretion in chondrosteans could be different from that described for teleosteans.
authors.affiliation: Department of Zoology, School of Science, Campus Fuentenueva, University of Granada, 18071, Granada, Spain. chidalgo@ugr.es.
authors.forename: M C
authors.initials: MC
authors.lastname: Hidalgo
authors.affiliation: Department of Cell Biology, School of Science, Campus Fuentenueva, University of Granada, 18071, Granada, Spain.
authors.forename: C E
authors.initials: CE
authors.lastname: Trenzado
authors.affiliation: Department of Zoology, School of Science, Campus Fuentenueva, University of Granada, 18071, Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Furné
authors.affiliation: Department of Zoology, School of Science, Campus Fuentenueva, University of Granada, 18071, Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Beltrán
authors.affiliation: Department of Zoology, School of Science, Campus Fuentenueva, University of Granada, 18071, Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Manzaneda
authors.affiliation: Department of Zoology, School of Science, Campus Fuentenueva, University of Granada, 18071, Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Gallego
authors.affiliation: Department of I+D, Piscifactoría "Sierra Nevada" S.L., Riofrío, Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Domezain
authors.affiliation: Department of Zoology, School of Science, Campus Fuentenueva, University of Granada, 18071, Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Sanz
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D005399:Fishes / Q000145:classification* / Q000378:metabolism*; D006854:Hydrocortisone / Q000378:metabolism; D015227:Lipid Peroxidation; D018384:Oxidative Stress / Q000502:physiology*; D013045:Species Specificity
chemical_list: D006854:Hydrocortisone
keywords: Brain; Cortisol; Fish; Liver; Oxidative status; Plasma