title: Comparative Susceptibility of Different Mouse Strains to Liver-Stage Infection With Plasmodium berghei Sporozoites Assessed Using In Vivo Imaging.
abstract: The liver stages of Plasmodium parasites are important targets for the discovery and development of prophylactic drugs.
  
  
  
  A real-time in vivo imaging system was used to determine the level of luminescence measured from firefly luciferase expression by sporozoites developing in hepatocytes in different strains of mice.
  
  
  
  The luminescence values (photon counts/sec) measured from the anatomical liver location in the untreated mice infected with 10,000 Plasmodium berghei sporozoites were 8.15 × 105for C57BL/6 Albino, 2.12 × 105for C3H/HeNCrL, 0.91 × 105for C57BL/6 WT, 0.28 × 105for BALB/c, and 0.16 × 105for ICR/CD-1 mice. This data suggests that the C57BL/6 Albino strain is most susceptible to luminescent photon, mainly because the less light scattering and absorption from deeper tissues and the skin in the strain of mouse. The photon count observed in black C57BL/6 wild type mice was shown to be 88.83% lower compared to C57BL/6 Albino mice. Although the highest growth rate of sporozoites in hepatocytes was found for C57BL/6 wild type mice in this study, the black skin of this mouse significantly reduced parasite-associated bioluminescence.
  
  
  
  The minimal light scattering and absorption and also enhanced susceptibility to liver infection of C57BL/6 Albino mice makes this strain preferable sensitivity for discovery and development of prophylactic antimalarial drugs.
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Qigui
authors.initials: Q
authors.lastname: Li
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Lisa
authors.initials: L
authors.lastname: Xie
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Diana
authors.initials: D
authors.lastname: Caridha
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Norma
authors.initials: N
authors.lastname: Roncal
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Mark
authors.initials: M
authors.lastname: Hickman
authors.affiliation: Experimental Therapeutics Branch, Military Malaria Research Program, Walter Reed Army Institute of Research, 503 Robert Grant Avenue Silver Spring, MD 02910.
authors.forename: Lisa
authors.initials: L
authors.lastname: Read
mesh_terms: D000818:Animals; D004198:Disease Susceptibility / Q000503:physiopathology*; D005260:Female; D008099:Liver / Q000503:physiopathology*; D008297:Male; D051379:Mice / Q000469:parasitology*; D008807:Mice, Inbred BALB C / Q000469:parasitology; D008809:Mice, Inbred C3H / Q000469:parasitology; D008810:Mice, Inbred C57BL / Q000469:parasitology; D008813:Mice, Inbred ICR / Q000469:parasitology; D010962:Plasmodium berghei / Q000472:pathogenicity*