title: Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing.
abstract: Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.
authors.affiliation: Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College, Japan. Electronic address: kazu_imai@ndmc.ac.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Imai
authors.affiliation: Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Tarumoto
authors.affiliation: Department of Pediatric Emergency Medicine, Osaka City General Hospital, Osaka, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Amo
authors.affiliation: Department of Plastic Surgery, Osaka City General Hospital, Osaka, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Department of Infectious Diseases, Tokyo Metropolitan Bokutoh General Hospital, Tokyo, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.affiliation: Department of Infectious Diseases, Tokyo Metropolitan Bokutoh General Hospital, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kosaka
authors.affiliation: Division of Preparedness and Emerging Infections, Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College, Japan; Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Mikita
authors.affiliation: Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Sakai
authors.affiliation: Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan; Department of Microbiology, Saitama Medical University, Saitama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Murakami
authors.affiliation: Department of Computational Biology and Medical Science, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan.
authors.forename: Shigefumi
authors.initials: S
authors.lastname: Maesaki
authors.affiliation: Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan; Department of Microbiology, Saitama Medical University, Saitama, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Maeda
mesh_terms: D002675:Child, Preschool; D000076263:Communicable Diseases, Imported / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D007564:Japan; D007892:Leishmania braziliensis / Q000302:isolation & purification*; D007895:Leishmania tropica / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D058608:Nanopores; D021141:Nucleic Acid Amplification Techniques; D017422:Sequence Analysis, DNA; D013593:Syria; D014687:Venezuela
keywords: Cutaneous leishmaniasis;; Diagnosis; LAMP;; Leishmania braziliensis; Leishmania tropica; MinION™