title: Abrogated Cell Contact Guidance on Amino-Functionalized Microgrooves.
abstract: Topographical and chemical features of biomaterial surfaces affect the cell physiology at the interface and are promising tools for the improvement of implants. The dominance of the surface topography on cell behavior is often accentuated. Striated surfaces induce an alignment of cells and their intracellular adhesion-mediated components. Recently, it could be demonstrated that a chemical modification via plasma polymerized allylamine was not only able to boost osteoblast cell adhesion and spreading but also override the cell alignment on stochastically machined titanium. In order to discern what kind of chemical surface modifications let the cell forget the underlying surface structure, we used an approach on geometric microgrooves produced by deep reactive ion etching (DRIE). In this study, we systematically investigated the surface modification by (i) methyl-, carboxyl-, and amino functionalization created via plasma polymerization processes, (ii) coating with the extracellular matrix protein collagen-I or immobilization of the integrin adhesion peptide sequence Arg-Gly-Asp (RGD), and (iii) treatment with an atmospheric pressure plasma jet operating with argon/oxygen gas (Ar/O2). Interestingly, only the amino functionalization, which presented positive charges at the surface, was able to chemically disguise the microgrooves and therefore to interrupt the microtopography induced contact guidance of the osteoblastic cells MG-63. However, the RGD peptide coating revealed enhanced cell spreading as well, with fine, actin-containing protrusions. The Ar/O2-functionalization demonstrated the best topography handling, e.g. cells closely attached even to features such as the sidewalls of the groove steps. In the end, the amino functionalization is unique in abrogating the cell contact guidance.
authors.affiliation: Department of Cell Biology, University Medical Center Rostock , Schillingallee 69, 18057 Rostock, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Mörke
authors.affiliation: Department of Cell Biology, University Medical Center Rostock , Schillingallee 69, 18057 Rostock, Germany.
authors.forename: Henrike
authors.initials: H
authors.lastname: Rebl
authors.affiliation: Leibniz-Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Strasse 2, 17489 Greifswald, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Finke
authors.affiliation: Biomaterials Department, INNOVENT e. V. , Pruessingstrasse 27B, 07745 Jena, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Dubs
authors.affiliation: Institute of Physics, University of Greifswald, Felix-Hausdorff-Strasse 6, 17487 Greifswald, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Nestler
authors.affiliation: Institute of Materials Sciences of Mulhouse (IS2M), CNRS UMR7361, 15 rue jean starcky, BP2488, 68057 Mulhouse cedex, France.
authors.forename: Aissam
authors.identifier: http://orcid.org/0000-0003-1839-0038
authors.initials: A
authors.lastname: Airoudj
authors.affiliation: Institute of Materials Sciences of Mulhouse (IS2M), CNRS UMR7361, 15 rue jean starcky, BP2488, 68057 Mulhouse cedex, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Roucoules
authors.affiliation: Biomaterials Department, INNOVENT e. V. , Pruessingstrasse 27B, 07745 Jena, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Schnabelrauch
authors.affiliation: Institute of Electronic Appliances and Circuits, University of Rostock , Albert-Einstein-Strasse 2, 18059 Rostock, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Körtge
authors.affiliation: Institute of Materials Sciences of Mulhouse (IS2M), CNRS UMR7361, 15 rue jean starcky, BP2488, 68057 Mulhouse cedex, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
authors.affiliation: Institute of Physics, University of Greifswald, Felix-Hausdorff-Strasse 6, 17487 Greifswald, Germany.
authors.forename: Christiane A
authors.initials: CA
authors.lastname: Helm
authors.affiliation: Department of Cell Biology, University Medical Center Rostock , Schillingallee 69, 18057 Rostock, Germany.
authors.forename: J Barbara
authors.identifier: http://orcid.org/0000-0003-2662-0905
authors.initials: JB
authors.lastname: Nebe
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D010006:Osteoblasts; D013499:Surface Properties; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D014025:Titanium
keywords: RGD; actin cytoskeleton; contact guidance; correlative microscopy; geometric microgrooves; physical plasma; water contact angle; zeta potential