title: Changes in miRNA in the lung and whole blood after whole thorax irradiation in rats.
abstract: We used a rat model of whole thorax x-ray irradiation to profile the microRNA (miRNA) in lung and blood up to 4 weeks after radiation. MiRNA from normal and irradiated Wistar rat lungs and whole blood were analyzed by next-generation sequencing and the changes by radiation were identified by differential deRNA-seq 1, 2, 3 and 4 weeks after irradiation. The average total reads/library was 2,703,137 with a mean of 88% mapping to the rat genome. Detailed profiles of 100 of the most abundant miRNA in rat blood and lung are described. We identified upregulation of 4 miRNA, miR-144-5p, miR-144-3p, miR-142-5p and miR-19a-3p in rat blood 2 weeks after radiation that have not previously been shown to be altered after radiation to the lung. Ingenuity Pathway Analysis identified signaling of inflammatory response pathways. These findings will support development of early detection methods, as well as mechanism(s) of injury and mitigation in patients after radiotherapy or radiological accidents.
authors.affiliation: Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Physiology, Medical College of Wisconsin,Milwaukee, WI, USA.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Jayashree
authors.initials: J
authors.lastname: Narayanan
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin,Milwaukee, WI, USA.
authors.forename: Meiying
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Brian L
authors.initials: BL
authors.lastname: Fish
authors.affiliation: Department of Physiology, Medical College of Wisconsin,Milwaukee, WI, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical College of Wisconsin,Milwaukee, WI, USA.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Liang
authors.affiliation: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, USA.|Research Service, Department of Veterans Affairs, Zablocki Veterans Affairs Medical Center, Milwaukee, WI, USA.
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Jacobs
authors.affiliation: Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI, USA.|Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, USA.|Research Service, Department of Veterans Affairs, Zablocki Veterans Affairs Medical Center, Milwaukee, WI, USA.
authors.forename: Meetha
authors.initials: M
authors.lastname: Medhora
mesh_terms: D000818:Animals; D005260:Female; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000097:blood*; D011833:Radiation Injuries, Experimental / Q000097:blood*; D051381:Rats; D013909:Thorax / Q000378:metabolism* / Q000473:pathology; D014965:X-Rays / Q000009:adverse effects*
chemical_list: D035683:MicroRNAs