title: NMR-Based Lipid Profiling of High Density Lipoprotein Particles in Healthy Subjects with Low, Normal, and Elevated HDL-Cholesterol.
abstract: Recent studies suggest that the cholesterol content of HDL (high density lipoproteins) may provide limited information on their antiatherogenic properties and that the composition and particles' structure provide more information on their functionality. We used NMR-based (nuclear magnetic resonance-based) lipidomics to study the relationships of serum HDL-C (HDL-cholesterol) levels with the lipid composition of HDL particles in three groups of subjects selected on the basis of their HDL-C levels. Subjects with low and high HDL-C levels exhibited differences in HDL lipidome compared to those with normal HDL-C levels. In pattern recognition analysis, the discrimination power among all groups was of high significance. The low HDL-C group presented enrichment of the core in triglycerides and depletion in cholesterol esters, whereas the high HDL-C group showed a decrease in triglycerides content. Additionally, as HDL-C increases, all lipid classes are esterified with higher percentage of unsaturated than saturated fatty acids. In addition to the aforementioned differences, the surface layer is enriched in sphingomyelin and free cholesterol in the high HDL-C level group. NMR-based lipidomic analysis of HDL can be particularly useful since it provides insights into molecular features and helps in the characterization of the atheroprotective function of HDL lipoproteins and in the identification of novel biomarkers of cardiovascular risk.
authors.affiliation: Laboratory of Clinical Chemistry and ‡Department of Internal Medicine, Faculty of Medicine, School of Health Sciences, University of Ioannina , 451 10, Ioannina, Greece.
authors.forename: Christina E
authors.identifier: 0000-0002-3784-0076
authors.initials: CE
authors.lastname: Kostara
authors.affiliation: Laboratory of Clinical Chemistry and ‡Department of Internal Medicine, Faculty of Medicine, School of Health Sciences, University of Ioannina , 451 10, Ioannina, Greece.
authors.forename: Vasilis
authors.initials: V
authors.lastname: Tsimihodimos
authors.affiliation: Laboratory of Clinical Chemistry and ‡Department of Internal Medicine, Faculty of Medicine, School of Health Sciences, University of Ioannina , 451 10, Ioannina, Greece.
authors.forename: Moses S
authors.initials: MS
authors.lastname: Elisaf
authors.affiliation: Laboratory of Clinical Chemistry and ‡Department of Internal Medicine, Faculty of Medicine, School of Health Sciences, University of Ioannina , 451 10, Ioannina, Greece.
authors.forename: Eleni T
authors.initials: ET
authors.lastname: Bairaktari
mesh_terms: D000328:Adult; D000368:Aged; D002318:Cardiovascular Diseases / Q000097:blood* / Q000235:genetics / Q000473:pathology; D008076:Cholesterol, HDL / Q000097:blood*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood; D010743:Phospholipids / Q000097:blood; D013109:Sphingomyelins / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008055:Lipids; D008075:Lipoproteins, HDL; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins
keywords: HDL; NMR; lipid composition; lipidomics; lipoproteins; phosphatidylcholine; phospholipids; sphingomyelin