title: MicroRNA-24 Modulates Staphylococcus aureus-Induced Macrophage Polarization by Suppressing CHI3L1.
abstract: Macrophages play a crucial role in host innate anti-Staphylococcus aureus defense, which is tightly regulated by multiple factors, including microRNAs. A recent study showed that miR-24 plays an important role in macrophage polarization. Here, we investigated the biological function of miR-24 in S. aureus-stimulated macrophages. The results revealed that miR-24 expression was significantly decreased in both human and mouse macrophage cell lines with S. aureus stimulation in a time-dependent manner. Moreover, miR-24 overexpression significantly decreased the production of M1 phenotype markers, such as IL-6, iNOS, TNF-α, CD86, and CD80, whereas it increased the production of M2 markers, such as Arg1, CCL17, CCL22, CD163, and CD206, in S. aureus-stimulated macrophages. Conversely, knockdown of miR-24 promoted M1 macrophage polarization but diminished M2 macrophage polarization in S. aureus-stimulated macrophages. Furthermore, CHI3L1 was predicted as a target gene of miR-24 using bioinformatics software and identified by luciferase reporter assay. Additionally, miR-24 overexpression inhibited CHI3L1 expression and downregulated the downstream MAPK pathway in S. aureus-stimulated macrophages. Finally, CHI3L1 overexpression rescued macrophage polarization and MAPK pathway inhibition induced by miR-24 mimic transfection in S. aureus-stimulated macrophages. In conclusion, the data suggest that miR-24 serves as a molecular regulator in S. aureus-induced macrophage polarization through targeting of CHI3L1 and regulation of the MAPK pathway, which may provide a promising therapeutic target for S. aureus-related infections and inflammatory diseases.
authors.affiliation: Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine in Henan Province, School of Laboratory Medicine, Xinxiang Medical University, No. 601 Jinsui Road, Hongqi District, Xinxiang, 453003, Henan Province, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jingjing
authors.affiliation: Xinxiang Medical University, Xinxiang, 453003, Henan Province, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Nan
authors.affiliation: The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453003, Henan Province, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Wei
authors.affiliation: Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine in Henan Province, School of Laboratory Medicine, Xinxiang Medical University, No. 601 Jinsui Road, Hongqi District, Xinxiang, 453003, Henan Province, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Qinghe
authors.affiliation: The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453003, Henan Province, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Weijuan
authors.affiliation: The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453003, Henan Province, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Peng
authors.affiliation: Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine in Henan Province, School of Laboratory Medicine, Xinxiang Medical University, No. 601 Jinsui Road, Hongqi District, Xinxiang, 453003, Henan Province, China. xiaopengwangxpw@163.com.
authors.forename: Wang
authors.initials: W
authors.lastname: Xiangpeng
mesh_terms: D000818:Animals; D002460:Cell Line; D000071451:Chitinase-3-Like Protein 1 / Q000037:antagonists & inhibitors*; D006801:Humans; D008262:Macrophage Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: C084533:CHI3L1 protein, human; C509289:Chil1 protein, mouse; D000071451:Chitinase-3-Like Protein 1; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; C529080:Mirn24 microRNA, mouse; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: CHI3L1; MAPK pathway; Staphylococcus aureus; macrophage polarization; miR-24