title: Exploring the Functions of 9-Lipoxygenase (DkLOX3) in Ultrastructural Changes and Hormonal Stress Response during Persimmon Fruit Storage.
abstract: Lipoxygenase (LOX) initiates the hydroperoxidation of polyunsaturated fatty acids and is involved in multiple physiological processes. In this study, investigation of various microscopic techniques showed that the fruit peel cellular microstructure of the two persimmon cultivars differed after 12 days of storage, resulting in fruit weight loss and an increased number and depth of microcracks. Analysis of subcellular localization revealed that greater amounts of DkLOX3-immunolabelled gold particles accumulated in "Fupingjianshi" than in "Ganmaokui" during storage. In addition, the expression ofDkLOX3was positively up-regulated by abscisic acid (ABA), concomitant with the promotion of ethylene synthesis and loss of firmness, and was suppressed by salicylic acid (SA), concomitant with the maintenance of fruit firmness, inhibition of ethylene production and weight loss. In particular, the expression ofDkLOX3differed from the ethylene trajectory after methyl jasmonate (MeJA) treatment. Furthermore, we isolated a 1105 bp 5' flanking region ofDkLOX3and the activity of promoter deletion derivatives was induced through various hormonal treatments. Promoter sequencecis-regulatory elements were analysed, and two conserved hormone-responsive elements were found to be essential for responsiveness to hormonal stress. Overall, these results will provide us with new clues for exploring the functions ofDkLOX3in fruit ripening and hormonal stress response.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. mengkunxn@163.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Meng
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. houyali1987@163.com.
authors.forename: Yali
authors.initials: Y
authors.lastname: Hou
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. hanyest1989@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. banqy1990@126.com.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Ban
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. hyh930111@163.com.
authors.forename: Yiheng
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. sjt_9527@163.com.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Suo
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. raojingpingxn@163.com.
authors.forename: Jingping
authors.initials: J
authors.lastname: Rao
mesh_terms: D001483:Base Sequence; D029788:Diospyros; D061353:Food Storage; D005638:Fruit / Q000378:metabolism / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008084:Lipoxygenase; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D010937:Plant Growth Regulators; D008084:Lipoxygenase
keywords: GUS activity; deletion derivatives; fruit peel; fruit ripening; immunolabelling; promoter