title: Sensitivity to oxazolone induced dermatitis is transferable with gut microbiota in mice.
abstract: Atopic Dermatitis (AD) has been associated with gut microbiota (GM) dysbiosis in humans, indicating a causative role of GM in AD etiology. Furthermore, the GM strongly correlates to essential disease parameters in the well-known oxazolone-induced mouse model of AD. Here, we demonstrate that it is possible to transfer both a high-responding and a low-responding AD phenotype with GM from conventional mice to germ-free mice. The mice inoculated with the high-responding GM had significantly higher clinical score, increased ear thickness, and increased levels of IL-1β, TNFα, IL-4, IL-5, and IL-6 compared to the mice inoculated with the low-responding GM. The inter-individual variation was in general not affected by this increase in effect size. Germ-free mice induced with AD revealed a high disease response as well as high inter-individual variation indicating protective properties of certain microbial taxa in this model. This study underlines that the GM has a strong impact on AD in mouse models, and that the power of studies may be increased by the application of mice inoculated with a specific GM from high responders to increase the effect size.
authors.affiliation: Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
authors.forename: Line Fisker
authors.initials: LF
authors.lastname: Zachariassen
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Denmark.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Krych
authors.affiliation: The Bartholin Institute, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Kåre
authors.initials: K
authors.lastname: Engkilde
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Denmark.
authors.forename: Dennis Sandris
authors.initials: DS
authors.lastname: Nielsen
authors.affiliation: Department of Environmental Science, Faculty of Science and Technology, Aarhus University, Denmark.
authors.forename: Witold
authors.initials: W
authors.lastname: Kot
authors.affiliation: Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
authors.forename: Camilla Hartmann Friis
authors.initials: CH
authors.lastname: Hansen
authors.affiliation: Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
authors.forename: Axel Kornerup
authors.initials: AK
authors.lastname: Hansen
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D004195:Disease Models, Animal; D064806:Dysbiosis / Q000382:microbiology* / Q000473:pathology; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D006801:Humans; D051379:Mice; D010081:Oxazolone / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D010081:Oxazolone