title: Transcriptional interference by RNA polymerase III affects expression of thePolr3egene.
abstract: Overlapping gene arrangements can potentially contribute to gene expression regulation. A mammalian interspersed repeat (MIR) nested in antisense orientation within the first intron of thePolr3egene, encoding an RNA polymerase III (Pol III) subunit, is conserved in mammals and highly occupied by Pol III. Using a fluorescence assay, CRISPR/Cas9-mediated deletion of the MIR in mouse embryonic stem cells, and chromatin immunoprecipitation assays, we show that the MIR affectsPolr3eexpression through transcriptional interference. Our study reveals a mechanism by which a Pol II gene can be regulated at the transcription elongation level by transcription of an embedded antisense Pol III gene.
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Meghdad
authors.initials: M
authors.lastname: Yeganeh
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland.|Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland.
authors.forename: Viviane
authors.initials: V
authors.lastname: Praz
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cousin
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Nouria
authors.initials: N
authors.lastname: Hernandez
mesh_terms: D000818:Animals; D017124:Conserved Sequence / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D053595:Embryonic Stem Cells; D005786:Gene Expression Regulation / Q000235:genetics*; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D007438:Introns / Q000235:genetics; D051379:Mice; D012319:RNA Polymerase II / Q000235:genetics; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D017384:Sequence Deletion
chemical_list: D016373:DNA, Antisense; D012333:RNA, Messenger; D012319:RNA Polymerase II; C000622088:Polr3e protein, mouse; D012320:RNA Polymerase III
keywords: Polr3e gene; RNA polymerase; SINE; antisense transcription; gene expression regulation; transcriptional interference