title: Therapy with Bone Marrow-Derived Autologous Adult Stem Cells in Quadriparesis due to Motor Neuron Disease.
abstract: OBJECTIVE
  To report the safety and therapeutic effectiveness of application of concentrated bone marrow aspirate in three bedridden patients with weakness in both legs, and monitor potential improvement in neurological outcomes.
  
  
  METHODS
  Case report. Intervention: Five infusions of 3x108mononuclear cells were administrated with 12 week intervals. Bone marrow (240ML) were obtained from the posterior superior iliac spine and Bone marrow mononuclear cells were enriched by standard manual close method under aseptic condition.
  
  
  RESULTS
  During the follow-up study of one year after stem cell implantation, the conditions of all three patients were improved and were confirmed by physical assessment, muscle charting and Electromyography (EMG). One year after stem cell implantation patients who were bedridden before treatment could sit without support and walk with support up to 200 feet at a stretch.
  
  
  CONCLUSIONS
  The local application of a cocktail of regenerative cell population found in an MNC fraction of bone marrow was safe and effective in improving quality of life and muscle strength in ALS patients. This case opens the need for further investigations on Autogenic stem cell transplant therapies for MND disease.
authors.affiliation: Mother Cell, Anupam Hospital, Rudrapur, Uttarakhand, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Bansal
authors.affiliation: Sir Ganga Ram Hospital, New Delhi, India.
authors.forename: Lipi
authors.initials: L
authors.lastname: Singh
authors.affiliation: Mother Cell, Anupam Hospital, Rudrapur, Uttarakhand, India.
authors.forename: Anupama
authors.initials: A
authors.lastname: Agrawal
authors.affiliation: Advanced Health Institute, Puerto Rico, USA.
authors.forename: Jerry
authors.initials: J
authors.lastname: Leon
authors.affiliation: Department of Anthropology, Wayne State University, Detroit, Michigan, USA.
authors.forename: I Birgitta
authors.initials: IB
authors.lastname: Sundell
authors.affiliation: Haffkine Institute, Mumbai, India.
authors.forename: Prasad S
authors.initials: PS
authors.lastname: Koka
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology; D001854:Bone Marrow Cells / Q000166:cytology*; D004576:Electromyography; D006801:Humans; D008297:Male; D016472:Motor Neuron Disease / Q000150:complications*; D011782:Quadriplegia / Q000209:etiology* / Q000628:therapy*; D033581:Stem Cell Transplantation / Q000009:adverse effects; D014182:Transplantation, Autologous
keywords: autologous bone marrow; motor neurons; stem cells