title: Over-expression of CHAF1A in Epithelial Ovarian Cancer can promote cell proliferation and inhibit cell apoptosis.
abstract: Chromatin Assembly Factor 1, subunit A (CHAF1A) can regulate cell proliferation, DNA repair and epigenetic changes in embryonic stem cells and it has been reported that over-expression of CHAF1A is associated with several human diseases including cancer. However, the expression and function of CHAF1A in Epithelial Ovarian Cancer (EOC) are rarely reported at present. In this study, we found that the positive staining of CHAF1A in EOC was higher than that in normal tissues and over-expression of CHAF1A was strongly associated with cancer stage and lymph node metastasis. Knockdown of CHAF1A by siRNA in EOC inhibited cell proliferation, reduced colony formation, caused G0/G1 phase arrest and promoted cell apoptosis. Taken together, the high expression of CHAF1A promotes cell proliferation and inhibits cell apoptosis and CHAF1A may be developed as a prognosis biomarker and potential therapeutic target of EOC.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Xia
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Wenxue
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Fangqian
authors.initials: F
authors.lastname: Shen
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Jufang
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Na
authors.initials: N
authors.lastname: Du
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China.
authors.forename: Yunyan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital, 650# XinSongJiang Road, Shanghai, 201600, China. Electronic address: xixiaowei1966@126.com.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xi
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D000077216:Carcinoma, Ovarian Epithelial; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056504:Chromatin Assembly Factor-1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D014157:Transcription Factors
chemical_list: C489780:CNOT8 protein, human; D056504:Chromatin Assembly Factor-1; D014157:Transcription Factors
keywords: Apoptosis; CHAF1A; Cell cycle; Epithelial Ovarian Cancer; Proliferation