title: Differential heat shock gene expression in chick blastula.
abstract: The component areas of chick blastula show differential expression of heat shock genes. The area opaca (ao), marginal zone (mz) and central area (ca) components of the epiblast display distinct quantitative and minor qualitative differences in the heat-induced and heat-repressible proteins, but are clearly different from the primary hypoblast (endoderm) in their expression of a given stress protein (hsp) as a response to heat shock. The major proteins synthesized in the component areas of epiblast in response to heat shock include hsp 18, 24, 70 and 89 Kd. Two-dimensional electrophoresis shows that each of these proteins consists of multiple charged species. The hypoblast expresses only hsp 70 Kd at non-significant levels and shows marked inhibition in the level of synthesis of heat-shock-repressible proteins. Heat shock during the blastula stage results in an increase in the size of the blastoderm and disrupts normal embryonic development. The heat shock genes provide an important molecular marker, which attests to regional specification in the chick blastula.
authors.affiliation: Division of Genetics and Cell and Developmental Biology, Department of Biology, University of Patras, Patras, Greece.
authors.forename: Nikolas
authors.initials: N
authors.lastname: Zagris
authors.affiliation: Laboratory of General Biology, Medical School, University of Ioannina, Ioannina, Greece.
authors.forename: Demetrios
authors.initials: D
authors.lastname: Matthopoulos
keywords: Chick blastula; Differentiation; Heat shock; Region specific gene expression