title: Potential Vaccine Targets against Rabbit Coccidiosis by Immunoproteomic Analysis.
abstract: The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites ofEimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially withE. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedaesera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits withE. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Song
authors.affiliation: Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 21009, China.
authors.forename: Ronglian
authors.initials: R
authors.lastname: Dong
authors.affiliation: Nantong Entry-Exit Inspection and Quarantine Bureau, Nantong 226004, China.
authors.forename: Baofeng
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Jing
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Shunxing
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Liucheng
authors.initials: L
authors.lastname: Wu
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Shengcun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Miao
authors.affiliation: Laboratory Animal Center of Nantong University, Nantong 226001, China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Shao
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology*; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D019295:Computational Biology; D004539:Eimeria / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D016052:Protozoan Vaccines / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D020543:Proteome; D016052:Protozoan Vaccines
keywords: Eimeria stiedae; immunoproteomics; rabbit; sporozoite; vaccine target