title: Fabrication of Electrospun Mucoadhesive Membranes for Therapeutic Applications in Oral Medicine.
abstract: Oral mucosal lesions are related to several etiologies, including trauma, infection, and immunologic and neoplastic diseases. Their prevalence varies greatly depending on ethnicity, gender, and exposure to risk factors. Currently, most oral mucosal lesions are treated with creams, mouthwashes, or gels containing suitable drugs. However, topical medications may be relatively ineffective as they are removed rapidly from oral surfaces, limiting drug contact times. Systemic medications might be more effective but are associated with unacceptable off-target side effects. The aim of this study was to produce novel polymeric mucoadhesive membranes for therapeutic applications on the oral mucosa using electrospinning. Poly(vinylpyrrolidone) (PVP) and Eudragit RS100 (RS100) were used for the fabrication of membranes, whereas dextran (Dex) or poly(ethylene oxide) (PEO) particles were incorporated to enhance their mucoadhesive properties. An electrospun poly(caprolactone) (PCL) backing layer (BL) was added to create a dual-layer system. Solution properties were studied using rheometry, and membranes were characterized using differential thermal analysis and scanning electron microscopy. Solubility, surface hydrophobicity, and adhesion properties were also investigated. The solution viscosity varied depending on the composition and concentration, affecting fiber production. The addition of RS100 to PVP resulted in reduced membrane porosity and solubility, and increased surface hydrophobicity and in vitro adhesion times. Dex and PEO particles were located on the surface of the fibers. A PCL BL was successfully produced, with enhanced attachment between layers achieved through thermal treatment. PVP homopolymer membranes did not adhere to plastic or porcine mucosa, whereas PVP/RS100 membranes with and without PEO or Dex were tightly adherent. In conclusion, PVP and RS100 may be combined to tailor membrane properties. Furthermore, electrospinning facilitated the production of membranes consisting of mucoadhesive-fabricated fibers displaying increased surface area and long-lasting adhesive properties. These novel compositions exhibit great potential for the fabrication of mucoadhesive patches for therapeutic applications in oral medicine.
authors.affiliation: School of Clinical Dentistry, University of Sheffield , 19 Claremont Crescent, S10 2TA Sheffield, U.K.
authors.forename: Martin E
authors.initials: ME
authors.lastname: Santocildes-Romero
authors.affiliation: School of Clinical Dentistry, University of Sheffield , 19 Claremont Crescent, S10 2TA Sheffield, U.K.
authors.forename: Lucie
authors.initials: L
authors.lastname: Hadley
authors.affiliation: School of Clinical Dentistry, University of Sheffield , 19 Claremont Crescent, S10 2TA Sheffield, U.K.
authors.forename: Katharina H
authors.initials: KH
authors.lastname: Clitherow
authors.affiliation: Dermtreat ApS , Abildgaardsvej 174, 2830 Virum, Denmark.
authors.forename: Jens
authors.initials: J
authors.lastname: Hansen
authors.affiliation: School of Clinical Dentistry, University of Sheffield , 19 Claremont Crescent, S10 2TA Sheffield, U.K.
authors.forename: Craig
authors.identifier: http://orcid.org/0000-0001-9724-122X
authors.initials: C
authors.lastname: Murdoch
authors.affiliation: School of Clinical Dentistry, University of Sheffield , 19 Claremont Crescent, S10 2TA Sheffield, U.K.
authors.forename: Helen E
authors.initials: HE
authors.lastname: Colley
authors.affiliation: School of Clinical Dentistry, University of Sheffield , 19 Claremont Crescent, S10 2TA Sheffield, U.K.|Dermtreat ApS , Abildgaardsvej 174, 2830 Virum, Denmark.
authors.forename: Martin H
authors.initials: MH
authors.lastname: Thornhill
authors.affiliation: School of Clinical Dentistry, University of Sheffield , 19 Claremont Crescent, S10 2TA Sheffield, U.K.
authors.forename: Paul V
authors.initials: PV
authors.lastname: Hatton
mesh_terms: D000269:Adhesives; D000818:Animals; D008566:Membranes; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D013552:Swine; D014783:Viscosity
chemical_list: D000269:Adhesives; D011108:Polymers
keywords: electrospinning; membranes; mucoadhesive; oral medicine; oral patches