title: Amyloid-β oligomers transiently inhibit AMP-activated kinase and cause metabolic defects in hippocampal neurons.
abstract: AMP-activated kinase (AMPK) is a key player in energy sensing and metabolic reprogramming under cellular energy restriction. Several studies have linked impaired AMPK function to peripheral metabolic diseases such as diabetes. However, the impact of neurological disorders, such as Alzheimer disease (AD), on AMPK function and downstream effects of altered AMPK activity on neuronal metabolism have been investigated only recently. Here, we report the impact of Aβ oligomers (AβOs), synaptotoxins that accumulate in AD brains, on neuronal AMPK activity. Short-term exposure of cultured rat hippocampal neurons orex vivohuman cortical slices to AβOs transiently decreased intracellular ATP levels and AMPK activity, as evaluated by its phosphorylation at threonine residue 172 (AMPK-Thr(P)172). The AβO-dependent reduction in AMPK-Thr(P)172levels was mediated by glutamate receptors of theN-methyl-d-aspartate (NMDA) subtype and resulted in removal of glucose transporters (GLUTs) from the surfaces of dendritic processes in hippocampal neurons. Importantly, insulin prevented the AβO-induced inhibition of AMPK. Our results establish a novel toxic impact of AβOs on neuronal metabolism and suggest that AβO-induced, NMDA receptor-mediated AMPK inhibition may play a key role in early brain metabolic defects in AD.
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and.
authors.forename: Gisele S
authors.initials: GS
authors.lastname: Seixas da Silva
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and.
authors.forename: Helen M
authors.initials: HM
authors.lastname: Melo
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and.|the Institute of Biophysics Carlos Chagas Filho.
authors.forename: Mychael V
authors.initials: MV
authors.lastname: Lourenco
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Lyra E Silva
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and.
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: de Carvalho
authors.affiliation: the Divisions of Neurology/Epilepsy Program and.
authors.forename: Soniza V
authors.initials: SV
authors.lastname: Alves-Leon
authors.affiliation: Neurosurgery, Clementino Fraga Filho Hospital, Federal University of Rio De Janeiro, Rio de Janeiro 21941-902, Brazil.
authors.forename: Jorge M
authors.initials: JM
authors.lastname: de Souza
authors.affiliation: the Department of Neurobiology, Northwestern University, Evanston, Illinois 60208-3520, and.
authors.forename: William L
authors.initials: WL
authors.lastname: Klein
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and.
authors.forename: Wagner S
authors.initials: WS
authors.lastname: da-Silva
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and.|the Institute of Biophysics Carlos Chagas Filho.
authors.forename: Sergio T
authors.initials: ST
authors.lastname: Ferreira
authors.affiliation: From the Institute of Medical Biochemistry Leopoldo de Meis and felice@bioqmed.ufrj.br.|the Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Fernanda G
authors.initials: FG
authors.lastname: De Felice
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism
chemical_list: C545487:APP protein, human; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D051246:Glucose Transport Proteins, Facilitative; D007328:Insulin; D010446:Peptide Fragments; D016194:Receptors, N-Methyl-D-Aspartate; C075222:amyloid beta-protein (1-42); D000255:Adenosine Triphosphate; D055372:AMP-Activated Protein Kinases
keywords: AMP-activated kinase (AMPK); ATP; Alzheimer disease; GLUTs; N-methyl-d-aspartate receptor (NMDA receptor, NMDAR); amyloid-β oligomers; energy metabolism