title: Biocompatibility and Effectiveness Evaluation of a New Hemostatic Embolization Agent: Thrombin Loaded Alginate Calcium Microsphere.
abstract: Background. Until now, there has been no ideal embolization agent for hemorrhage in interventional treatment. In this study, the thrombin was encapsulated in alginate calcium microsphere using electrostatic droplet technique to produce new embolization agent: thrombin loaded alginate calcium microspheres (TACMs).Objectives. The present work was to evaluate the biocompatibility and hemostatic efficiency of TACMs.Methods.Cell cytotoxicity, hemolysis, and superselective embolization of dog liver arteries were performed to investigate the biocompatibility of TACMs. To clarify the embolic effect of TACMs mixed thrombus in vivo, hepatic artery injury animal model of 6 beagles was established and transcatheter artery embolization for bleeding was performed.Results. Coculture with VECs revealed the noncytotoxicity of TACMs, and the hemolysis experiment was negligible. Moreover, the histological study of TACMs in liver blood vessel showed signs of a slight inflammatory reaction. The results of transcatheter application of TACMs mixed thrombus for bleeding showed that the blood flow was shut down completely after the TACMs mixed thrombus was delivered and the postprocedural survival rate of animal models at 12 weeks was 100%.Conclusions. With their good biocompatibility and superior hemostatic efficiency, TACMs might be a promising new hemostatic agent with a wide range of potential applications.
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China; Electrocardiogram Laboratory, Dezhou People's Hospital, Dezhou, Shandong 253000, China.
authors.forename: Fengqi
authors.initials: F
authors.lastname: Xuan
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Rong
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liang
authors.affiliation: Department of Ophthalmology, Dezhou People's Hospital, Dezhou, Shandong 253000, China.
authors.forename: Xuwen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Jingyang
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Xiaozeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The General Hospital of Shenyang Military Region, Shenyang, Liaoning 110016, China.
authors.forename: Yaling
authors.identifier: 0000-0002-4790-9559
authors.initials: Y
authors.lastname: Han
mesh_terms: D000464:Alginates / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D004285:Dogs; D004621:Embolization, Therapeutic; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry; D006470:Hemorrhage / Q000188:drug therapy* / Q000473:pathology; D006487:Hemostasis; D006490:Hemostatics; D006499:Hepatic Artery / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008863:Microspheres; D013917:Thrombin / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000464:Alginates; D006490:Hemostatics; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D013917:Thrombin