title: Upregulated glutathione transferase omega-1 correlates with progression of urinary bladder carcinoma.
abstract: OBJECTIVE
  Newly discovered glutathione transferase omega 1 (GSTO1-1) plays an important role in the glutathionylation cycle, a significant mechanism of protein function regulation. GSTO1-1 expression pattern has not been studied in transitional cell carcinoma (TCC), as yet.
  
  
  METHODS
  A total of 56 TCC tumor and corresponding non-tumor specimens were investigated. Glutathione content and thioltransferase activity were measured spectrophotometrically. Protein-glutathione mixed disulfides were measured fluorimetrically. GSTO1-1 expression was determined by immunoblot and qPCR. Immunoprecipitation with GSTO1-1 antibody was followed by immunoblot using anti-GSTO1, GSTP1, c-Jun, JNK, Akt, phospho-Akt, and ASK1 antibody, while for the total S-glutathionylation levels non-reducing electrophoresis was performed.
  
  
  RESULTS
  The contents of reduced glutathione and thioltransferase activity were significantly increased in tumor compared to non-tumor tissue. The increased GSTO1 expression in tumor tissue showed clear correlation with grade and stage. However, decreased total protein glutathionylation level in tumor compared to non-tumor samples was found. Immunoprecipitation has shown an association of GSTO1-1 with GSTP1, Akt, phospho-Akt, and ASK1 proteins.
  
  
  CONCLUSIONS
  GSTO1 deglutathionylase activity suggests its potential important role in redox perturbations present in TCC. Increased GSTO1-1 expression might contribute to TCC development and/or progression supporting the notion that GSTO1-1 may be a promising novel cancer target.
authors.affiliation: a Institute of Medical and Clinical Biochemistry, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Tatjana
authors.identifier: http://orcid.org/0000-0003-1959-5115
authors.initials: T
authors.lastname: Djukic
authors.affiliation: a Institute of Medical and Clinical Biochemistry, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Simic
authors.affiliation: a Institute of Medical and Clinical Biochemistry, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Pljesa-Ercegovac
authors.affiliation: a Institute of Medical and Clinical Biochemistry, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Matic
authors.affiliation: a Institute of Medical and Clinical Biochemistry, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Sonja
authors.identifier: http://orcid.org/0000-0002-3743-1213
authors.initials: S
authors.lastname: Suvakov
authors.affiliation: a Institute of Medical and Clinical Biochemistry, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Coric
authors.affiliation: b Clinic of Urology, Clinical Centre of Serbia, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Dragicevic
authors.affiliation: a Institute of Medical and Clinical Biochemistry, Faculty of Medicine , University of Belgrade , Belgrade , Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Savic-Radojevic
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D010084:Oxidation-Reduction; D060888:Real-Time Polymerase Chain Reaction; D001743:Urinary Bladder / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione
keywords: Glutathione transferase omega 1; glutathione; glutathionylation; transitional cell carcinoma; uroepithelium