title: Fat2 and Lar Define a Basally Localized Planar Signaling System Controlling Collective Cell Migration.
abstract: Collective migration of epithelial cells underlies diverse tissue-remodeling events, but the mechanisms that coordinate individual cell migratory behaviors for collective movement are largely unknown. Studying the Drosophila follicular epithelium, we show that the cadherin Fat2 and the receptor tyrosine phosphatase Lar function in a planar signaling system that coordinates leading and trailing edge dynamics between neighboring cells. Fat2 signals from each cell's trailing edge to induce leading edge protrusions in the cell behind, in part by stabilizing Lar's localization in these cells. Conversely, Lar signals from each cell's leading edge to stimulate trailing edge retraction in the cell ahead. Fat2/Lar signaling is similar to planar cell polarity signaling in terms of sub-cellular protein localization; however, Fat2/Lar signaling mediates short-range communication between neighboring cells instead of transmitting long-range information across a tissue. This work defines a key mechanism promoting epithelial migration and establishes a different paradigm for planar cell-cell signaling.
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
authors.forename: Kari
authors.initials: K
authors.lastname: Barlan
authors.affiliation: Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
authors.forename: Maureen
authors.initials: M
authors.lastname: Cetera
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA; Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA. Electronic address: shorne@uchicago.edu.
authors.forename: Sally
authors.initials: S
authors.lastname: Horne-Badovinac
mesh_terms: D000818:Animals; D015820:Cadherins / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D016764:Cell Polarity; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D006080:Ovarian Follicle / Q000166:cytology; D000072417:Protein Domains; D055550:Protein Stability; D011554:Pseudopodia / Q000378:metabolism; D054557:Receptor-Like Protein Tyrosine Phosphatases / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D015820:Cadherins; D029721:Drosophila Proteins; C546978:kug protein, Drosophila; C117978:Lar protein, Drosophila; D054557:Receptor-Like Protein Tyrosine Phosphatases
keywords: Drosophila; Fat2; Lar; cadherin; collective cell migration; egg chamber; epithelium