title: Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145.
abstract: The Streptomyces genus is well known for its ability to produce bio-active secondary metabolites of great medical interest. However, the metabolic features accompanying these bio-productions remain to be defined. In this study, the comparison of related model strains producing differing levels of actinorhoddin (ACT), showed that S. lividans, a weak producer, had high TriAcylGlycerol (TAG) content indicative of a glycolytic metabolism. In contrast, the strong producer, S. coelicolor, was characterized by low TAG content, active consumption of its polyphosphate (PolyP) stores and extremely high ATP/ADP ratios. This indicated highly active oxidative metabolism that was correlated with induction of ACT biosynthesis. Interestingly, in conditions of phosphate limitation, the ppk mutant had TAG content and ACT production levels intermediary between those of S. lividans and S. coelicolor. This strain was characterized by high ADP levels indicating that Ppk was acting as an Adenosine Di Phosphate Kinase. Its absence resulted in energetic stress that is proposed to trigger an activation of oxidative metabolism to restore its energetic balance. This process, which is correlated with ACT biosynthesis, requires acetylCoA to fuel the Krebs cycle and phosphate for ATP generation by the ATP synthase coupled to the respiratory chain, resulting in low TAG and polyP content of the ACT producing strains.
authors.affiliation: Group "Energetic Metabolism of Streptomyces", Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, INRA, Université Paris-Saclay, F-91198, Gif-sur-Yvette Cedex, France.|Sorbonne Universités, UPMC Univ Paris 06, UFR 927, Sciences de la vie, F-75005, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Esnault
authors.affiliation: Group "Energetic Metabolism of Streptomyces", Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, INRA, Université Paris-Saclay, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Dulermo
authors.affiliation: Group "Energetic Metabolism of Streptomyces", Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, INRA, Université Paris-Saclay, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Aleksey
authors.initials: A
authors.lastname: Smirnov
authors.affiliation: Group "Energetic Metabolism of Streptomyces", Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, INRA, Université Paris-Saclay, F-91198, Gif-sur-Yvette Cedex, France.|Department of Microbiology, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Askora
authors.affiliation: Group "Energetic Metabolism of Streptomyces", Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, INRA, Université Paris-Saclay, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Michelle
authors.initials: M
authors.lastname: David
authors.affiliation: Laboratoire de Chimie Physique, Université Paris-Sud, F-91405, Orsay Cedex, France.
authors.forename: Ariane
authors.initials: A
authors.lastname: Deniset-Besseau
authors.affiliation: Group "Energetic Metabolism of Streptomyces", Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, INRA, Université Paris-Saclay, F-91198, Gif-sur-Yvette Cedex, France.
authors.forename: Ian-Barry
authors.initials: IB
authors.lastname: Holland
authors.affiliation: Group "Energetic Metabolism of Streptomyces", Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, INRA, Université Paris-Saclay, F-91198, Gif-sur-Yvette Cedex, France. marie-joelle.virolle@i2bc.paris-saclay.fr.
authors.forename: Marie-Joelle
authors.initials: MJ
authors.lastname: Virolle
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D006019:Glycolysis; D018384:Oxidative Stress; D011122:Polyphosphates / Q000378:metabolism; D064210:Secondary Metabolism; D048372:Streptomyces coelicolor / Q000378:metabolism*; D048170:Streptomyces lividans / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D011122:Polyphosphates; D014280:Triglycerides; C013390:actinorhodin; D000095622:Benzoisochromanequinones