title: Ultrasensitive Detection of DNA and Ramos Cell Using In Situ Selective Crystallization Based Quartz Crystal Microbalance.
abstract: Herein we introduce the first successful assay of biomolecule by in situ selective crystallization based quartz crystal microbalance (QCM). Selective crystallization of CaCO3on QCM sensor surface was utilized as an efficient mass amplification strategy and enhanced the sensitivity of QCM significantly. High specificity is guaranteed by the cooperation of two functional groups: -N(CH3)3and -COOH. Passivation of sensor surface is realized by self-assembly of -N(CH3)3, which effectively inhibited the nonspecific crystallization. The DNA target is detected through hybridization of probe DNA labeled with -COOH, which can effectively promot the in situ surface crystallization of CaCO3. The concentration of target DNA is reflected by the frequency shift of QCM which is directly induced by the surface crystallization. The selective crystallization based QCM platform is simple, straightforward, high sensitive, and high specific. We demonstrate the excellent LOD (2 aM DNA) and a linear range of 10aM to 1 nM for DNA. Detection of Ramos cells are also realized with a LOD of five cells and a linear range of 5-6000 cells.
authors.affiliation: Shandong Province Key Laboratory of Detection Technology of Tumor Markers, School of Chemistry and Chemical Engineering,, Linyi University , Linyi 276005, China.
authors.forename: Li-Shang
authors.initials: LS
authors.lastname: Liu
authors.affiliation: Shandong Province Key Laboratory of Detection Technology of Tumor Markers, School of Chemistry and Chemical Engineering,, Linyi University , Linyi 276005, China.|Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University , Jinan 250014, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wu
authors.affiliation: Shandong Province Key Laboratory of Detection Technology of Tumor Markers, School of Chemistry and Chemical Engineering,, Linyi University , Linyi 276005, China.
authors.forename: Shusheng
authors.identifier: 0000-0002-8416-8331
authors.initials: S
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques; D002119:Calcium Carbonate / Q000737:chemistry*; D045744:Cell Line, Tumor; D003460:Crystallization; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D010316:Particle Size; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties
chemical_list: D004273:DNA, Neoplasm; D002119:Calcium Carbonate