title: A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies.
abstract: Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by N-(3-dimethylaminopropyl)-N'-ethylcar-bodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to form probes. We used the IMB probe to separate the Brucella and labeled by the QD probe, and then detected the fluorescence intensity with a fluorescence spectrometer. The detection method takes 105 min with a limit of detection of 103CFU/mL and ranges from 10 to 105CFU/mL (R2= 0.9983), and it can be well used in real samples.
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Yushen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Public Health, Xuzhou Medical University, 221000, Xuzhou, China.
authors.forename: Dehui
authors.initials: D
authors.lastname: Yin
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, 130000, Changchun, China.
authors.forename: Renguo
authors.initials: R
authors.lastname: Xie
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Huiwen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Bao
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, 130021, Changchun, China. songxiuling@jlu.edu.cn.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Song
authors.affiliation: China-Japan Union Hospital, Jilin University, 130000, Changchun, China. songwz@jlu.edu.cn.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Song
keywords: Fluorescence; IgG; IgY; Immunomagnetic beads; Quantum dots