title: Antifungal Activity of the Ethanol Extract fromFlos Rosae Chinensiswith Activity against Fluconazole-Resistant ClinicalCandida.
abstract: This study was designed to investigate the antifungal activity of a hydroalcoholic extract fromFlos Rosae Chinensis(FRC) combined with fluconazole (FCZ) against clinical isolates ofCandida albicansresistant to FCZ. The minimum inhibitory concentration (MIC) of FRC was determined using a checkerboard microdilution assay. The synergistic effects of the combination of FRC and FCZ against clinical isolates ofC. albicansresistant to FCZ were further confirmed by constructing time-growth curves and performing an agar diffusion test. FRC alone exerted efficient antifungal activities againstC. albicanswithin a MIC80ranging from 20μg/ml to 40μg/ml. FRC failed to enhance the effects of FCZ against sensitiveC. albicansstrains, although it rendered FCZ-resistantC. albicansmore sensitive. These results were further confirmed by the result of in vivo study. Our study is the first to discover that FRC can inhibit the growth ofC. albicansto a certain degree. An FRC antifungal mechanism study showed that FRC strengthens FCZ to inhibit the action of ergosterol biosynthesis by promoting the transformation of lanosterol to eburicol, suggesting that the antifungal mechanism of FRC involves the inhibition of ergosterol biosynthesis.
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Lulu
authors.identifier: 0000-0003-1560-9508
authors.initials: L
authors.lastname: Zhang
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Baodi
authors.initials: B
authors.lastname: Dai
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yan
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: YongBing
authors.identifier: 0000-0002-2344-1832
authors.initials: Y
authors.lastname: Cao
authors.affiliation: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Yuan-Ying
authors.identifier: 0000-0003-1187-0434
authors.initials: YY
authors.lastname: Jiang