title: Selection of Cholesterol-Lowering Lactic Acid Bacteria and its Effects on Rats Fed with High-Cholesterol Diet.
abstract: High cholesterol level in serum is a major factor of influence for coronary heart disease. The cholesterol-lowering ability of lactic acid bacteria (LAB) without side effects makes them more and more attractive. Seventy-nine strains of LAB isolated from fermented food were screened in vitro for their ability to assimilate cholesterol. Then, ten strains which exhibited higher ability of cholesterol assimilation were investigated with the characteristics of acidic resistance, bile salt tolerance, and cell adhesion. According to the results, the best strain LP96 was picked out, and used to evaluate its effects on the high-cholesterol diet-fed rats. The results demonstrated that the levels of serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol, and liver TC and TG were reduced significantly in the groups that received the strain LP96 solution compared with the model group, and that the serum high-density lipoprotein cholesterol levels were increased without any significant difference. Furthermore, LP96 also showed good antioxidative activity and improvement of intestinal microbial balance in the rats. Thus, LP96 may be a promising probiotics with potential cholesterol-lowering ability.
authors.affiliation: Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China.
authors.forename: Fengchun
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.affiliation: Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China. zhyouyang@sdau.edu.cn.
authors.forename: Zhengyou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000220:Adaptation, Biological; D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects; D001647:Bile Acids and Salts / Q000494:pharmacology; D001835:Body Weight; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D059305:Diet, High-Fat; D000069196:Gastrointestinal Microbiome; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D010802:Phylogeny; D019936:Probiotics; D051381:Rats
chemical_list: D000975:Antioxidants; D001647:Bile Acids and Salts; D002784:Cholesterol