title: Sodium tanshinone IIA sulfonate prevents hypoxic trophoblast-induced endothelial cell dysfunction via targeting HMGB1 release.
abstract: Preeclampsia (PE) is a serious blood pressure disorder of pregnancy. Systemic endothelial cell dysfunction, a hallmark of PE, is previously estimated to be induced by hypoxic trophoblast high mobility group box 1 (HMGB1). In the present study, we investigated the protective effect of sodium tanshinone IIA sulfonate (STS), the soluble form of tanshinone IIA isolated from danshen, against hypoxic trophoblast HMGB1-induced human umbilical vein endothelial cell (HUVEC) dysfunction. Our results showed that HMGB1 expression and release were significantly decreased in STS-treated hypoxic JEG-3 cells. A further study revealed hypoxic trophoblast HMGB1-induced cytotoxicity and leukostasis of HUVEC as well as higher expression of cell adhesion molecules (VCAM-1 and ICAM-1) could be reversed by pretreatment with STS. In conclusion, our study suggests that STS is an effective agent against hypoxic trophoblast-induced cell injury of HUVEC via targeting HMGB1 release and forms the basis of the development of such a compound in treating PE.
authors.affiliation: Department of Obstetrics & Gynecology, Wuxi Maternity and Child Health Hospital Affiliated Nanjing Medical University, Wuxi, 214002, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics & Gynecology, Wuxi Maternity and Child Health Hospital Affiliated Nanjing Medical University, Wuxi, 214002, People's Republic of China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Obstetrics & Gynecology, Wuxi Maternity and Child Health Hospital Affiliated Nanjing Medical University, Wuxi, 214002, People's Republic of China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Pathology, Wuxi Maternity and Child Health Hospital Affiliated Nanjing Medical University, Wuxi, 214002, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Pathology, Wuxi Maternity and Child Health Hospital Affiliated Nanjing Medical University, Wuxi, 214002, People's Republic of China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Pathology, Wuxi Maternity and Child Health Hospital Affiliated Nanjing Medical University, Wuxi, 214002, People's Republic of China.
authors.forename: Daozhen
authors.initials: D
authors.lastname: Chen
mesh_terms: D015687:Cell Hypoxia / Q000187:drug effects; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D024243:HMGB1 Protein / Q000592:standards*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010616:Phenanthrenes / Q000494:pharmacology*; D011225:Pre-Eclampsia / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D010616:Phenanthrenes; C024894:tanshinone II A sodium sulfonate
keywords: high mobility group box 1 (HMGB1); preeclampsia; sodium tanshinone IIA sulfonate