title: Autophagosome formation: Where the secretory and autophagy pathways meet.
abstract: The upregulation of autophagosome formation in response to nutrient deprivation requires significant intracellular membrane rearrangements that are poorly understood. Recent findings have implicated COPII-coated vesicles, well known as ER-Golgi cargo transport carriers, as key players in macroautophagy. The role of COPII vesicles in macroautophagy and how they interact with autophagy-related (Atg) proteins was unknown. In our recent report, we show that during nutrient deprivation, phosphorylation of the membrane-distal surface of the COPII coat subunit Sec24 facilitates the interaction of Sec24 with the Atg machinery (specifically, Atg9) to regulate the abundance of autophagosomes during starvation. Phosphorylation of Sec24 is specifically required for macroautophagy, but not ER-Golgi transport. These findings begin to unravel the unique function of COPII vesicles during starvation-induced macroautophagy.
authors.affiliation: a College of Life Science and Bioengineering , Beijing University of Technology , Beijing , China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: b Department of Cellular and Molecular Medicine , University of California at San Diego , La Jolla , CA , USA.
authors.forename: Saralin
authors.initials: S
authors.lastname: Davis
authors.affiliation: b Department of Cellular and Molecular Medicine , University of California at San Diego , La Jolla , CA , USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhu
authors.affiliation: c MRC Laboratory of Molecular Biology , Cambridge , UK.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Miller
authors.affiliation: b Department of Cellular and Molecular Medicine , University of California at San Diego , La Jolla , CA , USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Ferro-Novick
mesh_terms: D000818:Animals; D000071182:Autophagosomes / Q000378:metabolism*; D001343:Autophagy / Q000502:physiology*; D001692:Biological Transport / Q000502:physiology*; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D010588:Phagosomes / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: COPII vesicles; autophagosome formation; autophagy and secretory pathway crosstalk; cell homeostasis; phosphorylation