title: Hydroxyapatite reinforced inherent RGD containing silk fibroin composite scaffolds: Promising platform for bone tissue engineering.
abstract: Replacement and repair of ectopic bone defects and traumatized bone tissues are done using porous scaffolds and composites. The prerequisites for such scaffolds include high mechanical strength, osseoconductivity and cytocompatibility. The present work is designed to address such requirements by fabricating a reinforced cytocompatible scaffold. Biocompatible silk protein fibroin collected from tropical non-mulberry tasar silkworm (Antheraea mylitta) is used to fabricate fibroin-hydroxyapatite (HAp) nanocomposite particles using chemical precipitation method. In situ reinforcement of fibroin-HAp nanocomposite and external deposition of HAp particles on fibroin scaffold is carried out for comparative evaluations of bio-physical and biochemical characteristics. HAp deposited fibroin scaffolds provide greater mechanical strength and cytocompatibility, when compared with fibroin-HAp nanoparticles reinforced fibroin scaffolds. Minimal immune responses of both types of composite scaffolds are observed using osteoblast-macrophage co-culture model. Nanocomposite reinforced fibroin scaffold can be tailored further to accommodate different requirements depending on bone type or bone regeneration period.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur.
authors.forename: Sibaram
authors.initials: S
authors.lastname: Behera
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur.
authors.forename: Deboki
authors.initials: D
authors.lastname: Naskar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur.
authors.forename: Sunaina
authors.initials: S
authors.lastname: Sapru
authors.affiliation: Department of Material Science, Indian Institute of Technology, Kharagpur.
authors.forename: Promita
authors.initials: P
authors.lastname: Bhattacharjee
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur; Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University. Electronic address: tulidey@unipune.ac.in.
authors.forename: Tuli
authors.initials: T
authors.lastname: Dey
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur.
authors.forename: Ananta K
authors.initials: AK
authors.lastname: Ghosh
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology, Kharagpur.
authors.forename: Mahitosh
authors.initials: M
authors.lastname: Mandal
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur. Electronic address: kundu@hijli.iitkgp.ernet.in.
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones; D017886:Durapatite; D005349:Fibroins; D053758:Nanoparticles; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D005349:Fibroins; D017886:Durapatite
keywords: Bone tissue engineering; HAp; Nanoparticles; Non-mulberry silk fibroin