title: Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method.
abstract: Ceftriaxone is a cephalosporin antibiotic with a potent antimicrobial activity and excellent penetration in most body fluids such as pleural, peritoneal, spinal and brain. These facts contribute to the application of ceftriaxone in the treatment of bacterial peritonitis, an abdominal disorder in veterinary medicine, with potential risk of death. The determination of ceftriaxone levels in plasma and peritoneal fluid may be used to assess the pharmacokinetic profile at various instances of administration and allows observing if the concentrations needed are being achieved. Therefore a method was developed and validated for the determination of ceftriaxone in plasma and peritoneal fluid which after was applied in a pharmacokinetic profile study. The bioanalytical method validation was performed according to widely acceptable experiments. Two horses were used as a model of the method applicability; ceftriaxone was intraperitoneally administered to these animals as a single dose. The plasma and peritoneal fluid analysis were performed using an UHPLC system in reverse phase chromatography mode in fully validated conditions. The methods have shown linearity between 0.49 and 500μg/mL for plasma, and between 0.24 and 500μg/mL for peritoneal fluid. The quantitative analysis of ceftriaxone in these matrices allows monitoring of the therapy. This method showed improved sensitivity as well as the quantitation in peritoneal fluid.
authors.affiliation: Department of Natural Active Principles and Toxicology, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Araraquara, Sao Paulo, Brazil.
authors.forename: Michel Leandro
authors.initials: ML
authors.lastname: Campos
authors.affiliation: Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, UNESP-Univ Estadual Paulista, Botucatu, Sao Paulo, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: de Moura Alonso
authors.affiliation: Department of Natural Active Principles and Toxicology, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Araraquara, Sao Paulo, Brazil.
authors.forename: Evelin
authors.initials: E
authors.lastname: Dos Santos Martins
authors.affiliation: Department of Natural Active Principles and Toxicology, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Araraquara, Sao Paulo, Brazil.
authors.forename: Jonata Augusto
authors.initials: JA
authors.lastname: Oliveira
authors.affiliation: Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, UNESP-Univ Estadual Paulista, Botucatu, Sao Paulo, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Hussni
authors.affiliation: Department of Natural Active Principles and Toxicology, School of Pharmaceutical Sciences, UNESP-Univ Estadual Paulista, Araraquara, Sao Paulo, Brazil. Electronic address: peccinin@fcfar.unesp.br.
authors.forename: Rosângela Gonçalves
authors.initials: RG
authors.lastname: Peccinini
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D001202:Ascitic Fluid / Q000737:chemistry*; D002443:Ceftriaxone / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D006736:Horses; D010949:Plasma / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D002443:Ceftriaxone
keywords: Bioanalytical method validation; Ceftriaxone; Pharmacokinetics; UHPLC