title: Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration.
abstract: Zebrafish can regenerate after brain injury, and the regenerative process is driven by resident stem cells. Stem cells are heterogeneous in the vertebrate brain, but the significance of having heterogeneous stem cells in regeneration is not understood. Limited availability of specific stem cells might impair the regeneration of particular cell lineages. We studied regeneration of the adult zebrafish cerebellum, which contains two major stem and progenitor cell types: ventricular zone and neuroepithelial cells. Using conditional lineage tracing we demonstrate that cerebellar regeneration depends on the availability of specific stem cells. Radial glia-like cells are thought to be the predominant stem cell type in homeostasis and after injury. However, we find that radial glia-like cells play a minor role in adult cerebellar neurogenesis and in recovery after injury. Instead, we find that neuroepithelial cells are the predominant stem cell type supporting cerebellar regeneration after injury. Zebrafish are able to regenerate many, but not all, cell types in the cerebellum, which emphasizes the need to understand the contribution of different adult neural stem and progenitor cell subtypes in the vertebrate central nervous system.
authors.affiliation: Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 01307, Germany jan.kaslin@monash.edu.|Australian Regenerative Medicine Institute, Monash University, Innovation Walk 15, Lvl1, 3800, Clayton, Melbourne, Australia.
authors.forename: Jan
authors.identifier: 0000-0002-6207-1889
authors.initials: J
authors.lastname: Kaslin
authors.affiliation: Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 01307, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Kroehne
authors.affiliation: Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 01307, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Ganz
authors.affiliation: Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 01307, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hans
authors.affiliation: Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 01307, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Brand
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D019070:Cell Lineage; D002531:Cerebellum / Q000473:pathology / Q000502:physiology*; D006706:Homeostasis; D008954:Models, Biological; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D046569:Neuroepithelial Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D012038:Regeneration; D015027:Zebrafish / Q000502:physiology*
keywords: Cerebellum; Neural stem cell; Neurogenesis; Radial glia; Regeneration; Zebrafish