title: Isolation of infectious microalga Prototheca wickerhamii from a carp (Cyprinus carpio) - a first confirmed case report of protothecosis in a fish.
abstract: Protothecosis is a rare infection caused by environmentally ubiquitous achlorophyllic microalgae of the genus Prototheca. Here, we describe a first case of protothecosis in a carp (Cyprinus carpio), which is at the same time the first case of protothecosis in a fish, confirmed by phenotype- and molecular-based methods, including PCR sequencing of the rDNA cluster and protein profiling using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: T
authors.identifier: http://orcid.org/0000-0001-9553-5742
authors.initials: T
authors.lastname: Jagielski
authors.affiliation: Department of Genetics, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Dyląg
authors.affiliation: Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Berlin, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Roesler
authors.affiliation: Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Murugaiyan
mesh_terms: D000818:Animals; D017756:Aquaculture; D002347:Carps; D048028:DNA, Algal / Q000235:genetics; D005393:Fish Diseases / Q000209:etiology*; D007239:Infections / Q000209:etiology / Q000662:veterinary*; D008297:Male; D058086:Microalgae / Q000235:genetics / Q000302:isolation & purification; D011525:Prototheca / Q000235:genetics / Q000302:isolation & purification*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D048028:DNA, Algal; D012337:RNA, Ribosomal, 18S
keywords: Cyprinus carpio; Prototheca wickerhamii; algae; carp; protothecosis