title: Difference in protein synthesis of ovaries indicates predetermined sex inChrysomya rufifacies (Diptera, Calliphoridae).
abstract: Sex in the monogenic blowflyChrysomya rufifacies is under the control of a germ-line autonomous maternal effect sex realizer. In order to identify the ovarian poly(A)+RNA that might be related to sex predetermination, we analysed the patterns of cell-free translation products of total poly(A)+RNA from female and male predetermined ovaries and oocytes. During vitellogenesis we observed one transient sex-linked difference in a stable pattern composed of more than 800 in vitro translated proteins. This difference, however, was no longer detectable in mature oocytes.
authors.affiliation: Biologiezentrum, Zoologisches Institut der Universität, Olshausenstraße 40-60, D-2300, Kiel, Federal Republic of Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Kirchhoff
keywords: Maternal effect; Oogenesis; Sex determination; Two-dimensional protein patterns