title: Sizing Up Protein-Ligand Complexes: The Rise of Structural Mass Spectrometry Approaches in the Pharmaceutical Sciences.
abstract: Capturing the dynamic interplay between proteins and their myriad interaction partners is critically important for advancing our understanding of almost every biochemical process and human disease. The importance of this general area has spawned many measurement methods capable of assaying such protein complexes, and the mass spectrometry-based structural biology methods described in this review form an important part of that analytical arsenal. Here, we survey the basic principles of such measurements, cover recent applications of the technology that have focused on protein-small-molecule complexes, and discuss the bright future awaiting this group of technologies.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109; email: bruotolo@umich.edu.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Eschweiler
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109; email: bruotolo@umich.edu.
authors.forename: Richard
authors.initials: R
authors.lastname: Kerr
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109; email: bruotolo@umich.edu.
authors.forename: Jessica
authors.initials: J
authors.lastname: Rabuck-Gibbons
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109; email: bruotolo@umich.edu.
authors.forename: Brandon T
authors.initials: BT
authors.lastname: Ruotolo
mesh_terms: D055808:Drug Discovery; D007477:Ions / Q000737:chemistry; D008024:Ligands; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*
chemical_list: D007477:Ions; D008024:Ligands; D004364:Pharmaceutical Preparations; D011506:Proteins
keywords: collision-induced unfolding; drug discovery; ion mobility; native mass spectrometry; structural biology