title: Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression.
abstract: This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >0.05 μg/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however, did not affect CYP450 3A37 mRNA levels. Supplementation with 2 μM SeMet protected against AFB1-induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in primary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Xingxiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Chaoping
authors.initials: C
authors.lastname: Che
authors.affiliation: Institute for Cell and Molecular Biosciences, Newcastle University , Newcastle upon Tyne NE4 5PL, U.K.
authors.forename: Viktor I
authors.initials: VI
authors.lastname: Korolchuk
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Gan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Pan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Kehe
authors.identifier: http://orcid.org/0000-0003-4132-3052
authors.initials: K
authors.lastname: Huang
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens; D065607:Cytochrome P-450 Enzyme Inhibitors / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D012645:Selenomethionine / Q000494:pharmacology*; D051151:Selenoprotein W / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D030161:Avian Proteins; D065607:Cytochrome P-450 Enzyme Inhibitors; D051151:Selenoprotein W; D003577:Cytochrome P-450 Enzyme System; D012645:Selenomethionine; D016604:Aflatoxin B1; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: AFB1; cytochrome P450 enzymes; primary chicken hepatocytes; selenomethionine; selenoprotein W; siRNA