title: IP3R-mediated Ca2+release regulates protein metabolism inDrosophilaneuroendocrine cells: implications for development under nutrient stress.
abstract: Successful completion of animal development is fundamentally reliant on nutritional cues. Surviving periods of nutritional insufficiency requires adaptations that are coordinated, in part, by neural circuits. As neuropeptides secreted by neuroendocrine (NE) cells modulate neural circuits, we investigated NE cell function during development under nutrient stress. StarvedDrosophilalarvae exhibited reduced pupariation if either insulin signaling or IP3/Ca2+signaling were downregulated in NE cells. Moreover, an IP3R (inositol 1,4,5-trisphosphate receptor) loss-of-function mutant displayed reduced protein synthesis, which was rescued by overexpression of either InR (insulin receptor) or IP3R in NE cells of the mutant, suggesting that the two signaling pathways might be functionally compensatory. Furthermore, cultured IP3R mutant NE cells, but not neurons, exhibited reduced protein translation. Thus cell-specific regulation of protein synthesis by IP3R in NE cells influences protein metabolism. We propose that this regulation helps developing animals survive in poor nutritional conditions.
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India.
authors.lastname: Megha
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India gaiti@ncbs.res.in.
authors.forename: Gaiti
authors.identifier: 0000-0001-7194-383X
authors.initials: G
authors.lastname: Hasan
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D005502:Food; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D009154:Mutation / Q000235:genetics; D055099:Neuroendocrine Cells / Q000166:cytology* / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D011679:Pupa / Q000187:drug effects / Q000254:growth & development; D011972:Receptor, Insulin / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects; D013395:Sucrose / Q000494:pharmacology
chemical_list: D053496:Inositol 1,4,5-Trisphosphate Receptors; D013395:Sucrose; D011972:Receptor, Insulin; D002118:Calcium
keywords: ER Ca2+ stores; Insulin signaling; Pupariation; Starvation; dILP5