title: Genetic dissection of the maize (Zea mays L.) MAMP response.
abstract: Loci associated with variation in maize responses to two microbe-associated molecular patterns (MAMPs) were identified. MAMP responses were correlated. No relationship between MAMP responses and quantitative disease resistance was identified. Microbe-associated molecular patterns (MAMPs) are highly conserved molecules commonly found in microbes which can be recognized by plant pattern recognition receptors. Recognition triggers a suite of responses including production of reactive oxygen species (ROS) and nitric oxide (NO) and expression changes of defense-related genes. In this study, we used two well-studied MAMPs (flg22 and chitooctaose) to challenge different maize lines to determine whether there was variation in the level of responses to these MAMPs, to dissect the genetic basis underlying that variation and to understand the relationship between MAMP response and quantitative disease resistance (QDR). Naturally occurring quantitative variation in ROS, NO production, and defense genes expression levels triggered by MAMPs was observed. A major quantitative traits locus (QTL) associated with variation in the ROS production response to both flg22 and chitooctaose was identified on chromosome 2 in a recombinant inbred line (RIL) population derived from the maize inbred lines B73 and CML228. Minor QTL associated with variation in the flg22 ROS response was identified on chromosomes 1 and 4. Comparison of these results with data previously obtained for variation in QDR and the defense response in the same RIL population did not provide any evidence for a common genetic basis controlling variation in these traits.
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, China.|Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA.
authors.forename: Xinye
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Division of Plant Science and Biochemistry, University of Missouri, Columbia, MO, 65211, USA.|Laboratorio de Genomica Funcional de Leguminosas, FES Iztacala, UNAM, Tlalnepantla, 54090, Mexico.
authors.forename: Oswaldo
authors.initials: O
authors.lastname: Valdés-López
authors.affiliation: Statistics Department, North Carolina State University, Raleigh, NC, 27695, USA.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Arellano
authors.affiliation: Division of Plant Science and Biochemistry, University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Stacey
authors.affiliation: Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA. Peter.Balint-Kurti@ars.usda.gov.|U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) Plant Science Research Unit, Raleigh, NC, USA. Peter.Balint-Kurti@ars.usda.gov.
authors.forename: Peter
authors.identifier: http://orcid.org/0000-0002-3916-194X
authors.initials: P
authors.lastname: Balint-Kurti
mesh_terms: D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D014644:Genetic Variation; D009569:Nitric Oxide / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics*; D040641:Quantitative Trait Loci; D017382:Reactive Oxygen Species / Q000378:metabolism; D051192:Receptors, Pattern Recognition / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D017382:Reactive Oxygen Species; D051192:Receptors, Pattern Recognition; D009569:Nitric Oxide