title: Alternative Splicing Profile and Sex-Preferential Gene Expression in the Female and Male Pacific Abalone Haliotis discus hannai.
abstract: In order to characterize the female or male transcriptome of the Pacific abalone and further increase genomic resources, we sequenced the mRNA of full-length complementary DNA (cDNA) libraries derived from pooled tissues of female and maleHaliotis discus hannaiby employing the Iso-Seq protocol of the PacBio RSII platform. We successfully assembled whole full-length cDNA sequences and constructed a transcriptome database that included isoform information. After clustering, a total of 15,110 and 12,145 genes that coded for proteins were identified in female and male abalones, respectively. A total of 13,057 putative orthologs were retained from each transcriptome in abalones. Overall Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyzed in each database showed a similar composition between sexes. In addition, a total of 519 and 391 isoforms were genome-widely identified with at least two isoforms from female and male transcriptome databases. We found that the number of isoforms and their alternatively spliced patterns are variable and sex-dependent. This information represents the first significant contribution to sex-preferential genomic resources of the Pacific abalone. The availability of whole female and male transcriptome database and their isoform information will be useful to improve our understanding of molecular responses and also for the analysis of population dynamics in the Pacific abalone.
authors.affiliation: Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung 25457, Korea. kimmiaecho@gmail.com.
authors.forename: Mi Ae
authors.initials: MA
authors.lastname: Kim
authors.affiliation: Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 22012, Korea. jsrhee@inu.ac.kr.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.affiliation: Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung 25457, Korea. kio1231@naver.com.
authors.forename: Tae Ha
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Korea. ljs@chonnam.ac.kr.
authors.forename: Jung Sick
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Phyzen Genomics Institute, Seongnam 13558, Korea. ahyoung@phyzen.com.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Choi
authors.affiliation: Phyzen Genomics Institute, Seongnam 13558, Korea. bschoi@phyzen.com.
authors.forename: Beom-Soon
authors.initials: BS
authors.lastname: Choi
authors.affiliation: Department of Agriculture and Life Industry, Kangwon National University, Chuncheon 24341, Korea. choii@kangwon.ac.kr.
authors.forename: Ik-Young
authors.initials: IY
authors.lastname: Choi
authors.affiliation: Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung 25457, Korea. ycsohn@gwnu.ac.kr.
authors.forename: Young Chang
authors.initials: YC
authors.lastname: Sohn
keywords: Haliotis discus hannai; PIS system; abalone; isoform; transcriptome