title: Enterococcus faeciumFC-K Derived from Kimchi Is a Probiotic Strain That Shows Anti-Allergic Activity.
abstract: A rise in the occurrence of allergic diseases is attributed to the dysregulated balance of type 1/type 2 immunity, where type 2 T-helper (Th2) cells predominate over type 1 T-helper (Th1) cells, leading to an abnormally increased production of IgE in response to unharmful antigens. Kimchi, a traditional Korean fermented food, is a rich source of beneficial lactic acid bacteria. In this study, we investigated the ability ofEnterococcus faeciumFC-K derived from kimchi to induce type I immunity in the presence of Th2 polarizing conditions in vitro and in vivo. Stimulation of mouse peritoneal macrophages withE. faeciumFC-K induced the production of tumor necrosis factor alpha, interleukin (IL)-6, and IL-12. Under the in vitro Th2 conditions in which splenic T cells were activated in the presence of IL-4,E. faeciumFC-K enhanced the ability of T cells to produce interferon (IFN)-γ. Using the ovalbumin (OVA)-induced allergy model, male BALB/c mice receivingE. faeciumFC-K reduced the serum level of total IgE, but not that of OVA-specific IgE. Furthermore, the population of activated splenic B cells during OVA immunization was decreased inE. faeciumFC-K-treated mice, accounting for a reduction of total IgE in the serum. Restimulating splenocytes from OVA-immunized mice with OVA ex vivo resulted in an increased production of IFN-γ, with no effect on IL-4, inE. faeciumFC-Ktreated mice. These observations provide the evidence thatE. faeciumFC-K can be a beneficial probiotic strain that can modulate the Th2-mediated pathologic response.
authors.affiliation: Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Man-Kwang
authors.initials: MK
authors.lastname: Rho
authors.affiliation: Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Kim
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Hyun-In
authors.initials: HI
authors.lastname: Rho
authors.affiliation: Cellbion Co., Ltd, Sungnam 13605, Republic of Korea.
authors.forename: Tae-Rahk
authors.initials: TR
authors.lastname: Kim
authors.affiliation: Department of Oriental Dermatology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Yoon-Bum
authors.initials: YB
authors.lastname: Kim
authors.affiliation: Department of Oriental Dermatology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Sung
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Taw-Woo
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Dae-Ok
authors.initials: DO
authors.lastname: Kim
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Hee
authors.initials: H
authors.lastname: Kang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D016984:Enterococcus faecium / Q000276:immunology* / Q000302:isolation & purification*; D000074421:Fermented Foods / Q000382:microbiology*; D005516:Food Microbiology; D006967:Hypersensitivity / Q000276:immunology / Q000628:therapy*; D007073:Immunoglobulin E / Q000097:blood; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D019936:Probiotics / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D018418:Th2 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: Enterococcus faecium; IgE; T cells; allergy; kimchi