title: Protective Effect of Amygdalin on LPS-Induced Acute Lung Injury by Inhibiting NF-κB and NLRP3 Signaling Pathways.
abstract: The acute lung injury (ALI) is a leading cause of morbidity and mortality in critically ill patients. Amygdalin is derived from the bitter apricot kernel, an efficacious Chinese herbal medicine. Although amygdalin is used by many cancer patients as an antitumor agent, there is no report about the effect of amygdalin on acute lung injury. Here we explored the protective effect of amygdalin on ALI using lipopolysaccharide (LPS)-induced murine model by detecting the lung wet/dry ratio, the myeloperoxidase (MPO) in lung tissues, inflammatory cells in the bronchoalveolar lavage fluid (BALF), inflammatory cytokines production, as well as NLRP3 and NF-κB signaling pathways. The results showed that amygdalin significantly reduced LPS-induced infiltration of inflammatory cells and the production of TNF-α, IL-1β, and IL-6 in the BALF. The activity of MPO and lung wet/dry ratio were also attenuated by amygdalin. Furthermore, the western blotting analysis showed that amygdalin remarkably inhibited LPS-induced NF-κB and NLRP3 activation. These findings indicate that amygdalin has a protective effect on LPS-induced ALI in mice. The mechanism may be related to the inhibition of NF-κB and NLRP3 signaling pathways.
authors.affiliation: Department of Intensive Care Unit, First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Ao
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Intensive Care Unit, First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Pan
authors.affiliation: Department of Intensive Care Unit, First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Ophthalmology, First Hospital of Jilin University, Changchun, 130021, China. wuhuihuigz@sina.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced; D000678:Amygdalin / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein
chemical_list: D000678:Amygdalin; D000972:Antineoplastic Agents, Phytogenic; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011956:Receptors, Cell Surface; C492716:Nlrp3 protein, mouse; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein
keywords: ALI; LPS; NF-κB; NLRP3; amygdalin