title: Rapid Identification of Unknown Organic Iodine in Small-Volume Complex Biological Samples Based on Nanospray Mass Spectrometry Coupled with in-Tube Solid Phase Microextraction.
abstract: A new method for rapid screening of unknown organic iodine (OI) in small-volume complex biological samples was developed using in-tube solid phase microextraction (SPME) nanospray mass spectrometry (MS). The method proposed a new identification scheme for OI based on nanospray high-resolution mass spectrometry (HR-MS). The mass ranges of OI ions were confirmed using the t-MS2scan mode first; then, the possible precursor ions of OI were selected and identified orderly in full MS/ddMS2and t-MS2scan modes. Besides, in-tube SPME was used for the pretreatment of small-volume biological samples, and it was the first time in-tube SPME combined with nanospray MS for OI identification. The whole analysis procedure took only 8 min and consumed 50 μL per sample. Using the new method, six kinds of OI added to urine and an unknown OI C12H23O11I in human milk were successfully identified. Moreover, the proposed identification scheme is also suitable for other ambient mass spectrometry (AMS) to determine unknown compounds with characteristic fragment ions.
authors.affiliation: Faculty of Materials Science and Chemistry, ‡State Key Laboratory of Biogeology and Environmental Geology, and §Faculty of Earth Sciences, China University of Geosciences , Wuhan 430074, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.affiliation: Faculty of Materials Science and Chemistry, ‡State Key Laboratory of Biogeology and Environmental Geology, and §Faculty of Earth Sciences, China University of Geosciences , Wuhan 430074, P.R. China.
authors.forename: Yue'e
authors.identifier: 0000-0002-1278-7918
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Faculty of Materials Science and Chemistry, ‡State Key Laboratory of Biogeology and Environmental Geology, and §Faculty of Earth Sciences, China University of Geosciences , Wuhan 430074, P.R. China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Yun
authors.affiliation: Faculty of Materials Science and Chemistry, ‡State Key Laboratory of Biogeology and Environmental Geology, and §Faculty of Earth Sciences, China University of Geosciences , Wuhan 430074, P.R. China.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Faculty of Materials Science and Chemistry, ‡State Key Laboratory of Biogeology and Environmental Geology, and §Faculty of Earth Sciences, China University of Geosciences , Wuhan 430074, P.R. China.
authors.forename: Shenghong
authors.initials: S
authors.lastname: Hu
authors.affiliation: Faculty of Materials Science and Chemistry, ‡State Key Laboratory of Biogeology and Environmental Geology, and §Faculty of Earth Sciences, China University of Geosciences , Wuhan 430074, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Shuai
mesh_terms: D004105:Diiodotyrosine / Q000032:analysis*; D006801:Humans; D007462:Iodobenzenes / Q000032:analysis*; D013058:Mass Spectrometry; D008895:Milk, Human / Q000737:chemistry; D007470:Monoiodotyrosine / Q000032:analysis*; D036103:Nanotechnology; D052617:Solid Phase Microextraction; D013974:Thyroxine / Q000032:analysis*; D014285:Triiodothyronine, Reverse / Q000032:analysis*
chemical_list: C523964:3-iodotyrosine; D007462:Iodobenzenes; D014285:Triiodothyronine, Reverse; D004105:Diiodotyrosine; D007470:Monoiodotyrosine; D013974:Thyroxine