title: CUL5 and APOBEC3G Polymorphisms are Partially Implicated in HIV-1 Infection and Antiretroviral Therapy in a Brazilian Population.
abstract: BACKGROUND
  Host restriction factors are cellular proteins able to diminish or block viral replication in a cell-specific way.
  
  
  OBJECTIVE
  We evaluated the distribution of single nucleotide polymorphisms (SNPs) in APOBEC3G (rs3736685, rs2294367) and CUL5 (rs7117111, rs7103534, rs11212495) genes, among 264 HIV-1 infected (HIV-1+) and 259 unexposed- uninfected individuals from Northeast Brazil, looking for a possible association with susceptibility to HIV-1 infection, viral load during treatment, CD4+ T cell count and therapeutic success of the antiretroviral treatment.
  
  
  RESULTS
  The rs11212495 CUL5 G allele and the CUL5 rs7103534-rs7117111 CG haplotype were more frequent among unexposed-uninfected than in HIV-1+ individuals, suggesting an association with a lower HIV-1 infection susceptibility. The APOBEC3G rs2294367 G/C genotype correlated with delayed viral load suppression. Our results showed a great heterogeneity in relation to the literature findings, possibly due to ethnic differences among the studied populations, sample size used in the studies and, also, to the type of controls, i.e. in our study used unexposed-uninfected rather than exposed-uninfected individuals (rare and considered gold standard for susceptibility studies).
  
  
  CONCLUSIONS
  Our findings report genetic variants possibly associated with susceptibility to HIV-1 infection (CUL5 rs11212495, rs7103534, rs7117111) and partial viral load control (APOBEC3G rs2294367). Replica studies performed on higher number of subjects are envisaged to confirm our results.
authors.affiliation: Department of Genetics, Federal University of Pernambuco (UFPE), Recife. Brazil.|Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife. Brazil.
authors.forename: Ronaldo Celerino
authors.initials: RC
authors.lastname: da Silva
authors.affiliation: Department of Genetics, Federal University of Pernambuco (UFPE), Recife. Brazil.|Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife. Brazil.
authors.forename: Antonio Victor Campos
authors.initials: AVC
authors.lastname: Coelho
authors.affiliation: Department of Genetics, Federal University of Pernambuco (UFPE), Recife. Brazil.|Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife. Brazil.
authors.forename: Ronald Rodrigues
authors.initials: RR
authors.lastname: de Moura
authors.affiliation: Institute of Integral Medicine of Pernambuco Professor Fernando Figueira, Recife. Brazil.
authors.forename: Luiz Cláudio
authors.initials: LC
authors.lastname: Arraes
authors.affiliation: Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife. Brazil.|Department of Pathology, Federal University of Pernambuco (UFPE), Recife. Brazil.
authors.forename: Lucas André Cavalcanti
authors.initials: LAC
authors.lastname: Brandão
authors.affiliation: Department of Genetics, Federal University of Pernambuco (UFPE), Recife. Brazil.|Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife. Brazil.
authors.forename: Rafael Lima
authors.initials: RL
authors.lastname: Guimarães
authors.affiliation: Department of Genetics, Federal University of Pernambuco (UFPE), Recife. Brazil.|Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife. Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Crovella
mesh_terms: D000071480:APOBEC-3G Deaminase / Q000235:genetics*; D000328:Adult; D044966:Anti-Retroviral Agents / Q000627:therapeutic use*; D001938:Brazil; D018791:CD4 Lymphocyte Count; D044842:Cullin Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D015658:HIV Infections / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D016896:Treatment Outcome; D019562:Viral Load; D055815:Young Adult
chemical_list: D044966:Anti-Retroviral Agents; C496871:CUL5 protein, human; D044842:Cullin Proteins; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human
keywords: APOBEC3G; ART; CUL5; HIV-1; SNPs; viral load