title: Waterborne and Surface-Associated Carbohydrates as Settlement Cues for Larvae of the Specialist Marine Herbivore Alderia modesta.
abstract: Larvae of the specialist marine herbivore Alderia modesta (Opisthobranchia: Ascoglossa) metamorphose in response to a chemical settlement cue from the alga Vaucheria longicaulis, the obligate adult prey. Bioactivity coeluted with both high and low molecular weight carbohydrates in solution, and with insoluble high molecular weight carbohydrates associated with the algal cell wall. Larvae metamorphosed in response to water conditioned by V. longicaulis, as well as to frozen and homogenized algal tissue. The inducer was efficiently extracted from the algae with boiling water, but after all soluble activity was extracted, residual tissue still induced larval settlement. Ethanol precipitation of a boiled-water extract followed by gel filtration chromatography showed that the precipitate contained carbohydrates of >100,000 Da molecular weight, while the supernatant contained only low molecular weight carbohydrates (<2,000 Da); in both cases all activity was associated with the carbohydrate peak. An aqueous-insoluble 4% NaOH extract was chromatographed in 7 M urea to yield a bioactive high molecular weight carbohydrate peak. Activity was not affected by proteinase K or mild acid hydrolysis, but was significantly decreased by periodate treatment. The results indicate that larvae of A. modesta metamorphose in response to both water-soluble and surface-associated carbohydrates of V. longicaulis, and that the soluble cue exists as both high and low molecular weight isoforms.
authors.forename: P J
authors.initials: PJ
authors.lastname: Krug
authors.forename: A E
authors.initials: AE
authors.lastname: Manzi