title: An experimental and theoretical study on the interaction of DNA and BSA with novel Ni2+, Cu2+and VO2+complexes derived from vanillin bidentate Schiff base ligand.
abstract: In this investigation, the structure of bidentate N,N-Schiff base ligand of vanillin, (E)-4-(((2-amino-5-nitrophenyl)imino)methyl)-2-methoxyphenol (HL) was determined by single crystal X-ray diffraction. The interaction of new [CuL2], [NiL2] and [VOL2] complexes with DNA and BSA was explored through UV-Vis and fluorescence spectroscopy. The electronic spectra changes displayed an isosbestic point for the complexes upon titration with DNA. The Kbvalues for the complexes [CuL2], [NiL2] and [VOL2] were 2.4×105, 1.9×105and 4.2×104, respectively. [CuL2] complex was bound more toughly than [NiL2] and [VOL2] complexes. These complexes had a significant interaction with Bovine Serum Albumin (BSA) and the results demonstrated that the quenching mechanism was a static procedure. Also, the complexes interacted with BSA by more than one binding site (n>1). Finally, the theoretical studies were performed using the docking method to calculate the binding constants and recognize the binding site of the DNA and BSA with the complexes. The ligand and complexes including Ni2+, Cu2+and VO2+ions were colonized by fungal growth.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Dostani
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: akianfar@cc.iut.ac.ir.
authors.forename: Ali Hossein
authors.initials: AH
authors.lastname: Kianfar
authors.affiliation: School of Chemical Sciences, Universiti Sains Malaysia,11800 Minden, Penang, Malaysia.
authors.forename: Wan Ahmad Kamil
authors.initials: WA
authors.lastname: Mahmood
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Dinari
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Farrokhpour
authors.affiliation: Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Sabzalian
authors.affiliation: Department of Engineering, Ardakan University, Ardakan 89518-95491, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Abyar
authors.affiliation: School of Chemical Sciences, Universiti Sains Malaysia,11800 Minden, Penang, Malaysia.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Azarian
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D019216:Metals, Heavy / Q000737:chemistry / Q000378:metabolism; D062105:Molecular Docking Simulation; D011485:Protein Binding; D012545:Schiff Bases / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D001547:Benzaldehydes; D019216:Metals, Heavy; D012545:Schiff Bases; D012710:Serum Albumin, Bovine; D004247:DNA; C100058:vanillin
keywords: BSA; Bidentate N,N-Schiff base ligand; DNA; Fluorescence spectroscopy; Theoretical study