title: Bone Regeneration Using Adipose-Derived Stem Cells with Fibronectin in Dehiscence-Type Defects Associated with Dental Implants: An Experimental Study in a Dog Model.
abstract: OBJECTIVE
  To determine the bone regeneration potential of a ceramic biomaterial coated with fibronectin and adipose-derived stem cells covered in three-wall critical-size defects associated with dental implants.
  
  
  METHODS
  In a total of 18 dogs, four dehiscence-type and critical-size defects were created surgically in the edentulous alveolar ridge with the simultaneous placement of dental implants. Defects were randomly regenerated using biomaterials coated with particulate ß-tricalcium phosphate (β-TCP), β-TCP with fibronectin (Fn) (β-TCP-Fn), and β-TCP with a combination of Fn and autologous adipose-derived stem cells (ADSCs) (β-TCP-Fn-ADSCs), leaving one defect as the control. The animals were divided into three groups according to the time of euthanasia (1, 2, or 3 months).
  
  
  RESULTS
  Statistically significant differences between the three study groups (β-TCP, β-TCP-Fn, β-TCP-Fn-ADSCs) and the control group in the total area of bone regeneration and mineralized and nonmineralized tissue at 1, 2, and 3 months of healing were not observed. At 2 months, defects treated with β-TCP-Fn-ADSCs showed a significant decrease in the percentage of bone-to-implant contact (BIC) as compared with the β-TCP-Fn (P = .041) and control (P = .012) groups. At 3 months of healing, however, significant differences in BIC between the three study groups and controls were not found (P = .388).
  
  
  CONCLUSIONS
  The use of ADSCs in the bone regeneration processes of dehiscencetype defects associated with simultaneous implant insertion does not seem to improve the area of bone regeneration or the percentage of BIC compared with other biomaterials or the control alveolar defect.
authors.forename: Maria Àngels
authors.initials: MÀ
authors.lastname: Sánchez-Garcés
authors.forename: Joaquín
authors.initials: J
authors.lastname: Alvira-González
authors.forename: Claudia Müller
authors.initials: CM
authors.lastname: Sánchez
authors.forename: Joan R
authors.initials: JR
authors.lastname: Barbany Cairó
authors.forename: Manuel Reina
authors.initials: MR
authors.lastname: Del Pozo
authors.forename: Cosme
authors.initials: C
authors.lastname: Gay-Escoda
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D016301:Alveolar Bone Loss / Q000628:therapy*; D000539:Alveolar Process / Q000601:surgery; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000502:physiology*; D002130:Calcium Phosphates / Q000627:therapeutic use; D002516:Ceramics; D003758:Dental Implantation, Endosseous / Q000379:methods*; D015921:Dental Implants; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005353:Fibronectins / Q000627:therapeutic use*; D020007:Implants, Experimental; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D015921:Dental Implants; D005353:Fibronectins; C485817:beta-tricalcium phosphate