title: Rescue of nonlytic Newcastle Disease Virus (NDV) expressing IL-15 for cancer immunotherapy.
abstract: In order to test and enhance the antitumor activity against mice melanoma by NDV-modified tumor vaccine, a recombinant NDV expressing IL-15 (LX/(IL-15)) was generated by reverse genetics. Then, the expression level and biological activity of IL-15 were examined. Our results showed that mice tumor cell lines infected with LX/(IL-15) expressed IL-15 at a high level, and that expressed IL-15 was biologically active. Expression kinetics demonstrated that the highest expression level of IL-15 was at 48h post infection. The cytotoxicity assay showed that murine melanoma cells modified with LX/(IL-15) could significantly enhance the antitumor immune response in vitro. In vivo study also showed that murine melanoma cells modified with LX/(IL-15) could prevent melanoma growth in mice. Taken together, our data strongly indicated that recombinant LX/(IL-15) is a promising agent for cancer immunotherapy both for human and animal.
authors.affiliation: College of Basic Medicine and Biological Sciences, Medical Department, Soochow University, 215123 Suzhou, People's Republic of China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xu
authors.affiliation: Laboratory of Animal Infectious Diseases, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, People's Republic of China; Virus Research Unit, Department of Microbiology and Immunology, School of Medicine, University of Otago, New Zealand.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Basic Medicine and Biological Sciences, Medical Department, Soochow University, 215123 Suzhou, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Basic Medicine and Biological Sciences, Medical Department, Soochow University, 215123 Suzhou, People's Republic of China. Electronic address: zhaolixiang@suda.edu.cn.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D019409:Interleukin-15 / Q000235:genetics / Q000276:immunology*; D008546:Melanoma, Experimental / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D050130:Oncolytic Virotherapy / Q000379:methods; D050504:Oncolytic Viruses / Q000235:genetics / Q000276:immunology*; D059386:Reverse Genetics / Q000379:methods; D012878:Skin Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology; D019076:Transgenes
chemical_list: D019496:Cancer Vaccines; D019409:Interleukin-15
keywords: IL-15; Newcastle disease virus; Reverse genetics; Tumor; Vaccine