title: Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation.
abstract: OBJECTIVE
  The purpose of this study was to investigate IL-6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi.
  
  
  RESULTS
  Six LABs were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus lactis KR-050L inhibited IL-6-induced STAT3 luciferase activity. Fifteen compounds were isolated from the EtOAc extract of culture broth though column chromatography and preparative high-performance liquid chromatography, and they were identified as 2,5-diketopipperazine structures by spectroscopic analyses (MS,1H- and13C-NMR). They also showed inhibitory activities on IL-6-induced STAT3 activation, and showed the different in activity according to the presence of a phenylalanine residue, hydroxyl groups and isometric structure.
  
  
  CONCLUSIONS
  The six new LABs isolated from Gajuknamu kimchi, and Lc. lactis KR-050L was selected as candidate IL-6/STAT3 inhibitors. The activity levels of 15 2,5-DKPs isolated from Lc. lactis KR-050L were verified.
  
  
  CONCLUSIONS
  This study constitutes the first attempt to isolate various LABs from Gajuknamu kimchi and to discover IL-6/STAT3 inhibitors in the EtOAc extract of Lc. lactis KR-050L culture broth. Moreover, our data provide useful biochemical information regarding the commercialization of Lc. lactis isolated from Gajuknamu kimchi as an approach to use functional foods for the treatment of various diseases via IL-6/STAT3 activation.
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.|College of Agriculture and Life Sciences, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, Korea.
authors.forename: J T
authors.initials: JT
authors.lastname: Hwang
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.
authors.forename: C S
authors.initials: CS
authors.lastname: Park
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.affiliation: College of Agriculture and Life Sciences, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, Korea.
authors.forename: C-H
authors.initials: CH
authors.lastname: Lim
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.
authors.forename: S W
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk, 56212, Korea.
authors.forename: M-C
authors.initials: MC
authors.lastname: Rho
mesh_terms: D001685:Biological Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001937:Brassica / Q000382:microbiology*; D002460:Cell Line; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D013294:Lactococcus lactis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015394:Molecular Structure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014675:Vegetables / Q000382:microbiology*; D000093242:Interleukin-6 Inhibitors
chemical_list: D001685:Biological Factors; D015850:Interleukin-6; D012336:RNA, Ribosomal, 16S; D050796:STAT3 Transcription Factor; D000093242:Interleukin-6 Inhibitors
keywords: 2,5-diketopipperazine; Gajuknamu kimchi; IL-6; Lactococcus lactis KR-050L; STAT3