title: Evaluation of Surface Roughness and Bacterial Adhesion on Tooth Enamel Irradiated With High Intensity Lasers.
abstract: The aim was to evaluate the surface roughness and bacterial adhesion on enamel irradiated with high intensity lasers, associated or not to a fluoride varnish. Eighty fragments of bovine enamel were equally divided in 8 groups (n=10). Group 1 was not treated and Group 2 received only a 5% fluoride varnish application. The other groups were irradiated with an Er:Cr:YSGG (8.92 J/cm2), an Nd:YAG (84.9 J/cm2) and a diode laser (199.04 J/cm2), associated or not to a 5% fluoride varnish. The surface roughness was measured before and after treatments. Afterward, all samples were incubated in a suspension of S. mutans at 37 °C for 24 h. The colony-forming units (CFU) were counted by a stereoscope and the results were expressed in CFU/mm2. One-way ANOVA and the Tukey´s test compared the roughness data and the Student´s test compared the results obtained in the bacterial adhesion test (a=5%). The results showed that the irradiated samples without varnish presented the same roughness and the same bacterial adhesion that the non-irradiated samples. However, samples irradiated in the presence of fluoride varnish showed higher surface roughness and higher bacterial adhesion than the non-irradiated samples and those irradiated without varnish. Presence of pigments in the varnish increased the lasers' action on the enamel surface, which produced ablation in this hard tissue and significantly increased its surface roughness. For this reason, the enamel's susceptibility to bacterial adhesion was higher when the irradiation of the samples was made in presence of fluoride varnish.
authors.affiliation: Biopathology Division, Dental School, UNIUBE - Universidade de Uberaba, Uberaba, MG, Brazil.
authors.forename: Ruchele D
authors.initials: RD
authors.lastname: Nogueira
authors.affiliation: Biopathology Division, Dental School, UNIUBE - Universidade de Uberaba, Uberaba, MG, Brazil.
authors.forename: Camilla B
authors.initials: CB
authors.lastname: Silva
authors.affiliation: Clinical Dentistry, Dental School, UNIUBE - Universidade de Uberaba, Uberaba, MG, Brazil.
authors.forename: Cesar P
authors.initials: CP
authors.lastname: Lepri
authors.affiliation: Department of Restorative Dentistry, Ribeirão Preto Dental School, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Regina Guenka
authors.initials: RG
authors.lastname: Palma-Dibb
authors.affiliation: Clinical Dentistry, Dental School, UNIUBE - Universidade de Uberaba, Uberaba, MG, Brazil.
authors.forename: Vinicius R
authors.initials: VR
authors.lastname: Geraldo-Martins
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000528:radiation effects*; D002417:Cattle; D015169:Colony Count, Microbial; D003743:Dental Enamel / Q000528:radiation effects*; D066298:In Vitro Techniques; D053844:Lasers, Solid-State; D013295:Streptococcus mutans / Q000302:isolation & purification / Q000502:physiology / Q000528:radiation effects*; D013499:Surface Properties