title: Prostaglandin F2α-PTGFR signalling activation, growth factor expression and cell proliferation in bovine endometrial explants.
abstract: The endometrium of domestic animals undergoes regular periods of regeneration and degeneration and exhibits a remarkable capacity for self-repair during the oestrous cycle. The endometrial growth pattern is also observed during in the implantation period and early pregnancy, but the mechanism underlying endometrial growth in these processes remains unclear. A positive correlation between endometrial growth in these processes and prostaglandin (PG) F2αsecretion has been reported, but the roles that PGF2αplays in endometrial growth is less studied. In the present study, cell proliferation and the responses of a series of growth factors essential for endometrial growth to PGF2αreceptor (PTGFR) activation were investigated in bovine endometrial explants in vitro. Using real-time reverse transcription-polymerase chain reaction and western blotting, mRNA and protein expression of connective tissue growth factor, fibroblast growth factor2, interleukin8, matrix metalloproteinase2, transforming growth factor β1 and vascular endothelial growth factor A was increased (P<0.05) and cell proliferation, including epithelial and fibroblast proliferation, was induced in response to increased levels of proliferating cell nuclear antigen, cytokeratin-18 and fibroblast-specific protein-1 (P<0.05) following PTGFR activation by adding fluprostenol (10-9-10-5M) into culture medium of bovine endometrial explants. However, caspase-3 protein expression was reduced following treatment of explants with fluprostenol (10-9-10-5M, P<0.05). These results may help define the possible roles the PGF2α-PTGFR signalling pathway plays in endometrial growth.
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Shuangyi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Long
authors.initials: L
authors.lastname: Gao
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Mao
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Changqi
authors.initials: C
authors.lastname: Fu
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Duritahala
authors.initials: D
authors.lastname: Duritahala
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018, Hohhot, China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D011461:Prostaglandins F, Synthetic; D011982:Receptors, Prostaglandin; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D055513:Connective Tissue Growth Factor; C006326:fluprostenol; D015237:Dinoprost; D053148:Caspase 3; D020778:Matrix Metalloproteinase 2