title: Reverse lectin ELISA for detecting fucosylated forms of α1-acid glycoprotein associated with hepatocellular carcinoma.
abstract: Altered fucosylation of glycoproteins is associated with development of hepatocellular carcinoma (HCC). Lectins have been commonly used to assay changes in fucosylation of plasma glycoproteins. In the present study a recombinantly engineered form of the fucose binding lectin Aleuria aurantia (AAL) consisting of a single binding site for fucose (S2), was used to construct a reverse lectin ELISA method. Microtiter plates coated with the S2 lectin were used to capture glycoproteins from plasma samples followed by antibody detection of S2-bound fucosylated α1-acid glycoprotein (S2-bound AGP). The method was used to compare the level of S2-bound AGP in serum samples from a small cohort of patients with hepatitis, cirrhosis or HCC. Using the reverse S2 lectin ELISA it was shown that the levels of S2-bound AGP was significantly higher in HCC patients compared to non-cancer patients and that there was also a significant elevation of S2-bound AGP in HCC patients compared to cirrhosis patients. There was no correlation between the level of S2-bound AGP and total AGP concentration. The performance of S2-bound AGP in differentiating HCC from cirrhosis samples or hepatitis samples were compared to other markers. A combination of S2-bound AGP, α-fetoprotein and AGP concentration showed performances giving area under receiver operating curves of 0.87 and 0.95 respectively.
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Eva
authors.identifier: http://orcid.org/0000-0002-3258-4042
authors.initials: E
authors.lastname: Åström
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Stål
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Robin
authors.initials: R
authors.lastname: Zenlander
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Pia
authors.initials: P
authors.lastname: Edenvik
authors.affiliation: Department of Infectious Diseases, Kalmar County Hospital, Kalmar, Sweden.
authors.forename: Catharina
authors.initials: C
authors.lastname: Alexandersson
authors.affiliation: Department of Infectious Diseases, Kalmar County Hospital, Kalmar, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Haglund
authors.affiliation: Department of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden.
authors.forename: Ingvar
authors.initials: I
authors.lastname: Rydén
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Påhlsson
mesh_terms: D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005643:Fucose / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000737:chemistry*
chemical_list: D037102:Lectins; D009961:Orosomucoid; D005643:Fucose