title: Protein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP).
abstract: Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically different translation rates, in liveCaenorhabditis elegansanimals.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Pathology, NYU School of Medicine, New York, USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kourtis
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece.
authors.forename: Nektarios
authors.initials: N
authors.lastname: Tavernarakis
keywords: Caenorhabditis elegans; FRAP; Messenger RNA; Protein synthesis; Protein translation