title: Peptidomic Analysis of Fetal Heart Tissue for Identification of Endogenous Peptides Involved in Tetralogy of Fallot.
abstract: Tetralogy of fallot (TOF) is one of the most prevalent types of congenital heart diseases. As a category of bioactive molecules, peptides have been proved to participate in various biological processes. However, the role of endogenous peptides in the pathogenesis of TOF has not been studied. In this study, we performed a comparative peptidomic profile in the fetal heart of TOF and the control group for the first time by liquid chromatography-tandem mass spectrometry. Our data demonstrated that a total of 201 peptides derived from 176 precursor proteins were differentially expressed in the heart tissues of TOF fetuses compared with normal controls, including 41 upregulated peptides and 160 downregulated peptides. After analyzing the characteristics of these differentially expressed peptides and their precursor proteins, we found that these peptides were potentially involved in different biological processes, especially cardiogenesis and congenital anomaly of the cardiovascular system. Interestingly, we detected several extracellular matrix-derived peptides involved in our differentially expressed peptidomic profile. In summary, our study constructed a comparative peptidomic profile from the heart tissues of TOF fetuses and normal controls, and it identified a series of peptides that could potentially participate in heart development and TOF formation. The emergence of our peptidomics study indicated a new perspective to explore the pathogenesis of abnormal heart morphology, especially TOF.
authors.affiliation: 1 State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: 1 State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.affiliation: 2 Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Qing
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: 3 Department of Ultrasound, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.affiliation: 3 Department of Ultrasound, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 1 State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 4 Department of Pediatrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Shuping
authors.initials: S
authors.lastname: Han
authors.affiliation: 4 Department of Pediatrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Zhangbin
authors.initials: Z
authors.lastname: Yu
authors.affiliation: 5 Nanjing Maternal and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Xianwei
authors.initials: X
authors.lastname: Cui
authors.affiliation: 1 State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Tianhui
authors.initials: T
authors.lastname: Xu
authors.affiliation: 1 State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Dingyuan
authors.initials: D
authors.lastname: Ma
authors.affiliation: 1 State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.affiliation: 1 State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China .
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Xu
mesh_terms: D005260:Female; D005318:Fetal Heart / Q000378:metabolism* / Q000473:pathology; D063990:Gene Ontology; D006801:Humans; D010455:Peptides / Q000378:metabolism*; D011247:Pregnancy; D040901:Proteomics; D013771:Tetralogy of Fallot / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D010455:Peptides
keywords: fetal heart; liquid chromatography-tandem mass spectrometry; peptide; peptidomics; tetralogy of fallot