title: Detection of Matrix Metalloproteinases by Zymography.
abstract: Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis. They are also involved in the pathogenesis of a vast array of diseases, including but not limited to systemic inflammation, various cancers, and cardiovascular, neurological, and autoimmune diseases. Here, we describe gel zymography to detect MMPs in cell and tissue samples and in cell culture supernatants.
authors.affiliation: Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
authors.forename: Rajeev B
authors.initials: RB
authors.lastname: Tajhya
authors.affiliation: Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
authors.forename: Rutvik S
authors.initials: RS
authors.lastname: Patel
authors.affiliation: Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA. beeton@bcm.edu.
authors.forename: Christine
authors.initials: C
authors.lastname: Beeton
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000032:analysis*
chemical_list: D020782:Matrix Metalloproteinases
keywords: Detection; Protease; Semiquantitative; Zymogram; Zymography