title: The effect of tripterygium glucoside tablet on pharmacokinetics of losartan and its metabolite EXP3174 in rats.
abstract: Losartan and tripterygium glucoside tablet (TGT) are often simultaneously used for reducing urine protein excretion in clinic. However, it is unknown whether there is potential herb-drug interaction between losartan and TGT. The aim of this study was to investigate their potential herb-drug interaction, and clarify the mechanism of the effect of TGT on the pharmacokinetics of losartan and its metabolite EXP3174 in rats. The plasma concentrations of losartan and EXP3174 were determined by LC-MS, and the main pharmacokinetic parameters were calculated. The Cmax, t1/2and AUC(0-t)of losartan became larger after co-administration, while the Cmaxand AUC(0-t)of EXP3174 became smaller, suggesting that TGT could influence the pharmacokinetics of losartan and EXP3174. The effects of TGT and its main components on the metabolic rate of losartan were further investigated in rat liver microsomes. Results indicated that TGT and its two main ingredients could decrease the metabolic rate of losartan. Therefore, it was speculated that TGT might increase the plasma concentration of losartan and decrease the concentration of EXP3174 by inhibiting the metabolism of losartan. The results could provide references for clinical medication guidance of losartan and TGT to avoid the occurrence of adverse reactions.
authors.affiliation: The 118th Hospital of PLA, Wenzhou, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Hu
authors.affiliation: The 118th Hospital of PLA, Wenzhou, China.
authors.forename: Xuexue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The 118th Hospital of PLA, Wenzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.affiliation: The 118th Hospital of PLA, Wenzhou, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Shi
authors.affiliation: The 118th Hospital of PLA, Wenzhou, China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Ye
authors.affiliation: Department of Pharmacy, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Hai
authors.identifier: http://orcid.org/0000-0002-1318-780X
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005960:Glucosides / Q000737:chemistry / Q000493:pharmacokinetics*; D041743:Herb-Drug Interactions; D019808:Losartan / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013607:Tablets; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D005960:Glucosides; D010936:Plant Extracts; D013607:Tablets; C066026:losartan carboxylic acid; D019808:Losartan
keywords: LC-MS; herb-drug interaction; losartan; pharmacokinetics; tripterygium glucoside tablet