title: Properties of modified surface for biosensing interface.
abstract: Properties of modified surface, behavior against salting-out effect, suppressive effect for protein nonspecific adsorption, and wettability were examined using various mercapto compounds bearing methyloligoethylene glycol, oligoethylene glycol, alkyl oligoethylene glycol, alkyl phosphoryl choline, alkyl inverse phosphoryl choline, and alkyl sulfobetaine moieties. The behavior against salting-out effect was examined using gold nanoparticle with PBS and NaCl aqueous solution. The suppressive effect for protein nonspecific adsorption was evaluated by SPR, and the wettability was measured on the SPR chip. The gold nanoparticle modified with 8C3EG, 12C4EG, 12CPC, 6CCP, and 12CCP showed excellent behavior against salting-out effect. The suppression of protein nonspecific adsorption was effective with 6EG, 12C4EG, 12CPC, and 12CS. On the other hand, the modified surface possessed high wettability except for the surface modified with M6EG. The results indicate that incorporation of alkyl group into surface modification materials is effective for the enhancement of behavior against salting-out effect and suppressive effect for protein nonspecific adsorption regardless of wettability. Among the zwitter ionic derivatives, inverse phosphoryl choline derivatives showed intriguing properties, high behavior against salting-out effect with high wettability but low suppressive effect for protein nonspecific adsorption.
authors.affiliation: Health Research Institute, Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. Electronic address: mutsuo-tanaka@aist.go.jp.
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Health Research Institute, Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sawaguchi
authors.affiliation: Health Research Institute, Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hirata
authors.affiliation: Advanced Science Research Laboratory, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Niwa
authors.affiliation: School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Tawa
authors.affiliation: School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Sasakawa
authors.affiliation: Graduate School of Maritime Sciences, Kobe University, 5-1-1 Fukaeminami, Higashinada, Kobe, Hyogo 658-0022, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kuraoka
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
chemical_list: D013438:Sulfhydryl Compounds; D006046:Gold
keywords: Biosensing; Mercapto compound; Protein adsorption; Salting-out effect; Surface modification; Wettability