title: In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity.
abstract: The nervous system is composed of an enormous range of distinct neuronal types. These neuronal subpopulations are characterized by, among other features, their distinct dendritic morphologies, their specific patterns of axonal connectivity, and their selective firing responses. The molecular and cellular mechanisms responsible for these aspects of differentiation during development are still poorly understood. Here, we describe combined protocols for labeling and characterizing the structural connectivity and excitability of cortical neurons. Modification of the in utero electroporation (IUE) protocol allows the labeling of a sparse population of neurons. This, in turn, enables the identification and tracking of the dendrites and axons of individual neurons, the precise characterization of the laminar location of axonal projections, and morphometric analysis. IUE can also be used to investigate changes in the excitability of wild-type (WT) or genetically modified neurons by combining it with whole-cell recording from acute slices of electroporated brains. These two techniques contribute to a better understanding of the coupling of structural and functional connectivity and of the molecular mechanisms controlling neuronal diversity during development. These developmental processes have important implications on axonal wiring, the functional diversity of neurons, and the biology of cognitive disorders.
authors.affiliation: Department for Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC).
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Briz
authors.affiliation: Molecular Neurobiology Department, Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas (CSIC-UAM).
authors.forename: Marta
authors.initials: M
authors.lastname: Navarrete
authors.affiliation: Molecular Neurobiology Department, Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas (CSIC-UAM).
authors.forename: José A
authors.initials: JA
authors.lastname: Esteban
authors.affiliation: Department for Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC); mnlopez@cnb.csic.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Nieto
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D003712:Dendrites / Q000502:physiology; D018274:Electroporation / Q000379:methods*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques