title: Physical and biological properties of a novel anti-adhesion material made of thermally cross-linked gelatin film: Investigation of the usefulness as anti-adhesion material.
abstract: To create more useful, effective and safer anti-adhesion materials, we developed a thermally cross-linked gelatin film. In this study, we examined the physical properties of the film such as the physical strength and the adhesiveness to reveal the handling properties and biological properties, such as the anti-adhesion effect, the influence on cell proliferation, and the cytotoxicity to reveal the anti-adhesion mechanism, especially in comparison with the conventional hyaluronic acid and carboxymethylcellulose film (the conventional film). A tensile test under dry and wet conditions and shearing stress test showed that the gelatin film has significant higher maximum tensile stress and fracture strain than the conventional film. In the study using a rat model of cecum adhesion, the anti-adhesion effect of the gelatin film was significantly superior to that of the conventional film. In the cell proliferation test, the number of fibroblast cells on the gelatin film increased at each time point, while no cell proliferation was observed on the conventional film. Furthermore, in the cytotoxicity test using a colony assay and Live/Dead assay, the extract of the gelatin film had no cytotoxicity, while the extract of the conventional film had cytotoxicity considerably. These results suggest that the gelatin film provides better handling than the conventional film, due to better physical strength and ductility of the film. In addition, the gelatin film has a significantly greater anti-adhesion effect than the conventional film without any cytotoxicity. Therefore, the gelatin film is quite favorable as an anti-adhesion material. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 689-696, 2018.
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Tsunehito
authors.initials: T
authors.lastname: Horii
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsujimoto
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Yamanaka
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Torii
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ozamoto
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Takamori
authors.affiliation: Division of Biomedical Engineering, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Nakamachi
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ikada
authors.affiliation: Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
authors.forename: Akeo
authors.initials: A
authors.lastname: Hagiwara
mesh_terms: D000268:Adhesiveness / Q000187:drug effects*; D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry / Q000494:pharmacology; D002432:Cecum / Q000187:drug effects / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013718:Tensile Strength; D000267:Tissue Adhesions / Q000188:drug therapy / Q000473:pathology
chemical_list: D003432:Cross-Linking Reagents; D011108:Polymers; D005780:Gelatin; D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium
keywords: anti-adhesion effect; cytotoxicity; physical strength; thermally cross-linked gelatin film