title: Salmonella detection in powdered dairy products using a novel molecular tool.
abstract: In this study, we developed a rapid, specific, and sensitive loop-mediated isothermal amplification technique combined with a lateral flow dipstick (LAMP-LFD) method to detect Salmonella targeting the siiA gene in powdered infant formula (PIF). The specificity of the detection method (LAMP-LFD) approached 100% using 21 Salmonella and 31 non-Salmonella bacterial strains. This detection method exhibited high sensitivity limits for pure cultures at 3.7 cfu/mL and in PIF at 2.2 cfu/g without enrichment. To evaluate the applicability of the LAMP-LFD method, we detected 60 positive PIF samples and 20 negative PIF samples. The results showed that the method of LAMP-LFD had a high diagnostic specificity of 100% for detection of Salmonella in PIF. To reduce incidence of LAMP contamination, we applied propidium monoazide (PMA) to eliminate carryover contamination of LAMP. At the same time, we found that PMA does not affect observation of LFD for measurement of LAMP signal. The results verified that the method of LAMP-LFD targeting the siiA gene is rapid, accurate, and sensitive for Salmonella detection in PIF, and that PMA shows great potential to be widely used to eliminate the amplicon contamination risk generated by the highly sensitive LAMP reaction in the detection process.
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Pan
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Pang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China; National Research Center of Dairy Engineering and Technology, Northeast Agricultural University, Harbin, 150028, China. Electronic address: yujun_jiang@163.com.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address: mcxwh2006@qq.com.
authors.forename: Chaoxin
authors.initials: C
authors.lastname: Man
mesh_terms: D000818:Animals; D003611:Dairy Products; D041943:Infant Formula; D021141:Nucleic Acid Amplification Techniques; D012475:Salmonella / Q000235:genetics*; D012680:Sensitivity and Specificity
keywords: Salmonella; lateral flow dipstick; loop-mediated isothermal amplification; powdered infant formula