title: Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments.
abstract: BACKGROUND
  Guide Picker ( https://www.deskgen.com/guide-picker/ ) serves as a meta tool for designing CRISPR experiments by presenting ten different guide RNA scoring functions in one simple graphical interface. It allows investigators to simultaneously visualize and sort through every guide targeting the protein-coding regions of any mouse or human gene.
  
  
  RESULTS
  Utilizing a multidimensional graphical display featuring two plots and four axes, Guide Picker can analyze all guides while filtering based on four different criteria at a time. Guide Picker further facilitates the CRISPR design process by using pre-computed scores for all guides, thereby offering rapid guide RNA generation and selection.
  
  
  CONCLUSIONS
  The ease-of-use of Guide Picker complements CRISPR itself, matching a powerful and modular biological system with a flexible online web tool that can be used in a variety of genome editing experimental contexts.
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK. sorenh@desktopgenetics.com.
authors.forename: Soren H
authors.initials: SH
authors.lastname: Hough
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK.
authors.forename: Kris
authors.initials: K
authors.lastname: Kancleris
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK.
authors.forename: Leigh
authors.initials: L
authors.lastname: Brody
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Humphryes-Kirilov
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK.
authors.forename: Joseph
authors.initials: J
authors.lastname: Wolanski
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK.
authors.forename: Keith
authors.initials: K
authors.lastname: Dunaway
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK.
authors.forename: Ayokunmi
authors.initials: A
authors.lastname: Ajetunmobi
authors.affiliation: Desktop Genetics, Ltd., 28 Hanbury Street, London, E1 6QR, UK.
authors.forename: Victor
authors.initials: V
authors.lastname: Dillard
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D000072669:Gene Editing; D006801:Humans; D020407:Internet; D051379:Mice; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics / Q000378:metabolism*; D014584:User-Computer Interface
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems
keywords: CRISPR; Cas9; Functional genomics; Genome editing; RNA guide design software; sgRNA