title: miR-155 as a potential target of IL-3 signaling in primary AML cells.
abstract: miR-155 has emerged as one of the key microRNAs (miRNAs) involved in normal and malignant myelopoiesis, and high expression of this miRNA has been flagged as a strong independent prognostic marker in Acute Myeloid Leukemia (AML). While elevated expression of miR-155 has been associated with FLT3-ITD mutations, other mechanisms which may regulate expression of this miRNA in AML remain largely unknown. Here, we present new evidence that miR-155 may be a prime target of IL-3 signaling in primary AML cells. This finding, together with the increasingly apparent role for miR-155 in oncogenesis, and the upregulation of the IL-3 receptor alpha subunit in AML, lead us to propose this pathway may significantly contribute to the leukemic transformation.
authors.affiliation: Division of Haematology, Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia; School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, South Australia, Australia. Electronic address: teresa.sadras@sahmri.com.
authors.forename: Teresa
authors.initials: T
authors.lastname: Sadras
authors.affiliation: Division of Haematology, Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia; School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Chung H
authors.initials: CH
authors.lastname: Kok
authors.affiliation: Division of Haematology, Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia; School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Perugini
authors.affiliation: Division of Immunology, Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia.
authors.forename: Hayley S
authors.initials: HS
authors.lastname: Ramshaw
authors.affiliation: Division of Haematology, Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia; School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, South Australia, Australia; School of Pharmacy and Medical Sciences, The University of South Australia, Adelaide, South Australia, Australia.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: D'Andrea
mesh_terms: D002471:Cell Transformation, Neoplastic; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism*; D053650:Interleukin-3 Receptor alpha Subunit / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: C508596:IL3 protein, human; C115022:IL3RA protein, human; D007377:Interleukin-3; D053650:Interleukin-3 Receptor alpha Subunit; C524942:MIRN155 microRNA, human; D035683:MicroRNAs
keywords: AML; interleukin-3; miR-155