title: The 5'-AG5CC-3' Fragment from the Human CPEB3 Ribozyme Forms an Ultrastable Parallel RNA G-Quadruplex.
abstract: An unusually thermostable G-quadruplex is formed by a sequence fragment of a naturally occurring ribozyme, the human CPEB3 ribozyme. Strong evidence is provided for the formation of a uniquely stable intermolecular G-quadruplex structure consisting of five tetrad layers, by using CD spectroscopy, UV melting curves, 2D NMR spectroscopy, and gel shift analysis. The cationic porphyrin TMPyP4 destabilizes the complex.
authors.affiliation: LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk-Strasse 1, 53121, Bonn, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Kulikov
authors.affiliation: Institute for Inorganic Chemistry, University of Bonn, Gerhard-Domagk-Strasse 1, 53121, Bonn, Germany.
authors.forename: Senada
authors.initials: S
authors.lastname: Nozinovic
authors.affiliation: LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk-Strasse 1, 53121, Bonn, Germany.
authors.forename: Stephanie
authors.identifier: 0000-0002-5180-360X
authors.initials: S
authors.lastname: Kath-Schorr
mesh_terms: D002623:Chemistry Techniques, Analytical; D054856:G-Quadruplexes; D006801:Humans; D015394:Molecular Structure; D011166:Porphyrins / Q000494:pharmacology; D012313:RNA / Q000737:chemistry; D020871:RNA Stability / Q000187:drug effects; D016337:RNA, Catalytic / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry*
chemical_list: C513866:CPEB3 protein, human; D011166:Porphyrins; D016337:RNA, Catalytic; D016601:RNA-Binding Proteins; C021096:tetra(4-N-methylpyridyl)porphine; D012313:RNA
keywords: CPEB3 ribozyme; G-quadruplexes; RNA; RNA structures; circular dichroism