title: Chitosan nerve conduits seeded with autologous bone marrow mononuclear cells for 30 mm goat peroneal nerve defect.
abstract: In the current research, to find if the combination of chitosan nerve conduits seeded with autologous bone marrow mononuclear cells (BM-MNCs) can be used to bridge 30 mm long peroneal nerve defects in goats, 15 animals were separated into BM-MNC group (n = 5), vehicle group (n = 5), and autologous nerve graft group (n = 5). 12 months after the surgery, animals were evaluated by behavioral observation, magnetic resonance imaging tests, histomorphological and electrophysiological analysis. Results revealed that animals in BM-MNC group and autologous nerve graft group achieved fine functional recovery; magnetic resonance imaging tests and histomorphometry analysis showed that the nerve defect was bridged by myelinated nerve axons in those animals. No significant difference was found between the two groups concerning myelinated axon density, axon diameter, myelin sheath thickness and peroneal nerve action potential. Animals in vehicle group failed to achieve significant functional recovery. The results indicated that chitosan nerve conduits seeded with autologous bone marrow mononuclear cells have strong potential in bridging long peripheral nerve defects and could be applied in future clinical trials.
authors.affiliation: Department of tissue engineering, School of fundamental sciences, China Medical University, Shenyang, Liaoning Province, 110122, China.|Department of spine surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Aikeremujiang
authors.initials: A
authors.lastname: Muheremu
authors.affiliation: Department of Neurosurgery, Tsinghua University Yuquan Hospital, Beijing, 100049, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of tissue engineering, China Medical University, Shenyang, Liaoning Province, 110122, China.
authors.forename: Xiyuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of tissue engineering, School of fundamental sciences, China Medical University, Shenyang, Liaoning Province, 110122, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Neurosurgery, Tsinghua University Yuquan Hospital, Beijing, 100049, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Gong
authors.affiliation: Department of tissue engineering, School of fundamental sciences, China Medical University, Shenyang, Liaoning Province, 110122, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ao
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001853:Bone Marrow; D017690:Cell Transplantation / Q000379:methods*; D048271:Chitosan / Q000008:administration & dosage*; D004195:Disease Models, Animal; D006041:Goats; D006651:Histocytochemistry; D008279:Magnetic Resonance Imaging; D010523:Peripheral Nervous System Diseases / Q000628:therapy*; D010543:Peroneal Nerve / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan