title: Alterations of hepatocyte function with free radical generators and reparation or prevention with coffee polyphenols.
abstract: Liver diseases are linked in the majority of cases to oxidative stress that antioxidants could neutralize with reducing liver injury. Chlorogenic acid, a coffee polyphenol, possesses antioxidant prosperities. The aim of this study was to evaluate in vitro preventive and corrective effects of cholorogenic acid in hepatocyte toxicity induced by free radicals. Hepatocytes were isolated from adult male Wistar rats. To determine corrective effects and reparation, cells were first exposed to two free radical generators (hydrogen peroxide/iron sulfate for hydroxyl radical formation, and phenazine methosulfate/nicotinamide adenine dinucleotide for superoxide anion formation) for 12H and thereafter treated by chlorogenic acid (1 and 10 μM final concentration) for another 12H. To show preventive effects, cells were pretreated by chlorogenic acid and thereafter exposed to free radical generators. Hepatocyte proliferation, glucose uptake, ATP contents, membrane fluidity and integrity, and intracellular redox status were investigated after 24H culture. The results showed that chlorogenic acid reversed the decrease in cell proliferation, glucose uptake and ATP levels, the increased LDH release and the reduced membrane fluidity and restored the oxidant/antioxidant status under oxidative stress. When pre-treated with chlorogenic acid, hepatocytes became very resistant to oxidative conditions and cellular homeostasis was maintained. In conclusion, chlorogenic acid displayed not only corrective but also preventive effects in hepatocytes exposed to oxidative stress and could be beneficial in patients with or at risk of liver diseases.
authors.affiliation: a Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences , Earth and Universe, University ABOU-BEKR BELKAÏD , Tlemcen , Algeria.
authors.forename: Amel
authors.initials: A
authors.lastname: Saidi Merzouk
authors.affiliation: a Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences , Earth and Universe, University ABOU-BEKR BELKAÏD , Tlemcen , Algeria.
authors.forename: Merzouk
authors.initials: M
authors.lastname: Hafida
authors.affiliation: a Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences , Earth and Universe, University ABOU-BEKR BELKAÏD , Tlemcen , Algeria.
authors.forename: Amel
authors.initials: A
authors.lastname: Medjdoub
authors.affiliation: a Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences , Earth and Universe, University ABOU-BEKR BELKAÏD , Tlemcen , Algeria.
authors.forename: Bouchra
authors.initials: B
authors.lastname: Loukidi
authors.affiliation: a Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences , Earth and Universe, University ABOU-BEKR BELKAÏD , Tlemcen , Algeria.
authors.forename: Sabri
authors.initials: S
authors.lastname: Cherrak
authors.affiliation: b Department of Technical Sciences, Faculty of Engineering , University ABOU-BEKR BELKAÏD , Tlemcen , Algeria.
authors.forename: Sid Ahmed
authors.initials: SA
authors.lastname: Merzouk
authors.affiliation: c Laboratory of Bioorganic and Medicinal Chemistry , UMR 7509 CNRS - University of Strasbourg, ECPM , Strasbourg , Cedex 2 , France.
authors.forename: Mourad
authors.initials: M
authors.lastname: Elhabiri
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D002726:Chlorogenic Acid / Q000008:administration & dosage* / Q000737:chemistry; D003069:Coffee / Q000737:chemistry; D005609:Free Radicals / Q000008:administration & dosage / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003069:Coffee; D005609:Free Radicals; D059808:Polyphenols; D013481:Superoxides; D002726:Chlorogenic Acid; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide
keywords: Chlorogenic acid; culture; fluidity; hepatocytes; oxidant/antioxidant status; prevention; reparation