title: Diffuse fluorescence fiber probe for in vivo detection of circulating cells.
abstract: There has been significant recent interest in the development of technologies for enumeration of rare circulating cells directly in the bloodstream in many areas of research, for example, in small animal models of circulating tumor cell dissemination during cancer metastasis. We describe a fiber-based optical probe that allows fluorescence detection of labeled circulating cells in vivo in a diffuse reflectance configuration. We validated this probe in a tissue-mimicking flow phantom model in vitro and in nude mice injected with fluorescently labeled multiple myeloma cells in vivo. Compared to our previous work, this design yields an improvement in detection signal-to-noise ratio of 10 dB, virtually eliminates problematic motion artifacts due to mouse breathing, and potentially allows operation in larger animals and limbs.
authors.affiliation: Northeastern University, Department of Electrical and Computer Engineering, Boston, Massachusetts, United StatesbBoston University, Department of Biomedical Engineering, Boston, Massachusetts, United States.
authors.forename: Vivian
authors.initials: V
authors.lastname: Pera
authors.affiliation: Northeastern University, Department of Electrical and Computer Engineering, Boston, Massachusetts, United States.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Tan
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Center for Systems Biology and Wellman Center for Photomedicine, Boston, Massachusetts, United States.
authors.forename: Judith
authors.initials: J
authors.lastname: Runnels
authors.affiliation: Northeastern University, Department of Electrical and Computer Engineering, Boston, Massachusetts, United States.
authors.forename: Neha
authors.initials: N
authors.lastname: Sardesai
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Center for Systems Biology and Wellman Center for Photomedicine, Boston, Massachusetts, United States.
authors.forename: Charles P
authors.initials: CP
authors.lastname: Lin
authors.affiliation: Northeastern University, Department of Electrical and Computer Engineering, Boston, Massachusetts, United States.
authors.forename: Mark
authors.initials: M
authors.lastname: Niedre
mesh_terms: D000818:Animals; D002452:Cell Count / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis / Q000175:diagnosis; D009369:Neoplasms / Q000175:diagnosis; D009360:Neoplastic Cells, Circulating; D019047:Phantoms, Imaging
chemical_list: D005456:Fluorescent Dyes