title: [Detection of Autoantibodies Against the 1-Adrenergic Receptor in the Sera of Patients via the Competitive cell-Based Enzyme Linked Immunosorbent Assay].
abstract: This study aimed to assess the level of anti-1-adrenergic receptor autoantibodies in patients with ventricular arrhythmias with no signs of organic heart disease and with presence of cardiovascular pathology in comparison with a group of healthy volunteers.
  
  
  
  The study included 44 patients with ventricular arrhythmias with no signs of organic heart disease ("idiopathic"), 34 patients with diagnosed dilated cardiomyopathy (DCM) of inflammatory origin, 35 patients with coronary heart disease and ventricular arrhythmias, 12patients with coronary heart disease with no ventricular arrhythmias, and 19 healthy volunteers (control group). The level of autoantibodies against the 1-adrenergic receptor was determined by the developed competitive cell-based enzyme-linked immunosorbent assay (ELISA) and by the standard ELISA using peptides corresponding to the second extracellular loop of the 1-adrenergic receptor.
  
  
  
  Elevated level of autoantibodies detected by a competitive cell-based ELISA was observed in 62% of patients with DCM compared to 21% of healthy volunteers (p=0.0006). In patients with "idiopathic" ventricular arrhythmias, the level of 1-adrenergic receptor autoantibodies was lower than in healthy subjects (p=0.003). Coronary heart disease patients with or without ventricular arrhythmias exhibited no differences from the control group. The number of significantly positive signals in peptide-based ELISA did not exceed 10% in any of the groups. No correlation between the data from competitive cell-based ELISA and peptide-based ELISA was found.
  
  
  
  This study demonstrated that competitive cell-based ELISA technique can be applied for detection of 1-adrenergic receptor autoantibodies. The results in DCM patients generally correspond to the expected. Decreased level of autoantibodies in patients with "idiopathic" ventricular arrhythmias indicates that this disease is related to changes in the immune system. Such relation is not observed in the case of coronary heart disease patients.
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: A Y
authors.initials: AY
authors.lastname: Shevelev
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Kostiukevich
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Efremov
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Vlasik
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Mironova
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: K A
authors.initials: KA
authors.lastname: Zykov
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: N M
authors.initials: NM
authors.lastname: Kashirina
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: I B
authors.initials: IB
authors.lastname: Kuznetsova
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Sharf
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Mamochkina
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: L N
authors.initials: LN
authors.lastname: Lipatova
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Peklo
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: P N
authors.initials: PN
authors.lastname: Rutkevich
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Yanushevskaya
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: I N
authors.initials: IN
authors.lastname: Rybalkin
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Stukalova
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Malkina
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Belyaeva
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Kuznetsova
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Tkachev
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Zinchenko
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: E M
authors.initials: EM
authors.lastname: Gupalo
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: O Y
authors.initials: OY
authors.lastname: Agapova
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Yureneva-Tkhorzhevskaya
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Rvacheva
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Sidorova
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Sadgyan
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Tereshchenko
authors.affiliation: Russian Cardiology Scientific and Production Complex, Moscow, Russia.|Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Golitsyn
mesh_terms: D000328:Adult; D001145:Arrhythmias, Cardiac / Q000097:blood / Q000150:complications / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D002311:Cardiomyopathy, Dilated / Q000150:complications; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018342:Receptors, Adrenergic, beta-1 / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D018342:Receptors, Adrenergic, beta-1
keywords: 1-adrenergic receptor; autoantibodies; competitive cell-based ELISA; dilated cardiomyopathy; ventricular arrhythmias