title: Structure and Biophysics of CBFβ/RUNX and Its Translocation Products.
abstract: The core binding factor (CBF) transcription factor is somewhat unique in that it is composed of a DNA binding RUNX subunit (RUNX1, 2, or 3) and a non-DNA binding CBFβ subunit, which modulates RUNX protein activity by modulating the auto-inhibition of the RUNX subunits. Since the discovery of this fascinating transcription factor more than 20 years ago, there has been a robust effort to characterize the structure as well as the biochemical properties of CBF. More recently, these efforts have also extended to the fusion proteins that arise from the subunits of CBF in leukemia. This chapter highlights the work of numerous labs which has provided a detailed understanding of the structure and function of this transcription factor and its fusion proteins.
authors.affiliation: Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
authors.forename: Tahir H
authors.initials: TH
authors.lastname: Tahirov
authors.affiliation: Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, 22908, USA. jhb4v@virginia.edu.
authors.forename: John
authors.initials: J
authors.lastname: Bushweller
mesh_terms: D000818:Animals; D001703:Biophysics; D050657:Core Binding Factor alpha Subunits / Q000235:genetics* / Q000378:metabolism*; D050658:Core Binding Factor beta Subunit / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: D050657:Core Binding Factor alpha Subunits; D050658:Core Binding Factor beta Subunit; D004268:DNA-Binding Proteins; D014157:Transcription Factors
keywords: CBFβ; NMR; RUNX; Structure; X-ray