title: Early apoptosis of monocytes induced by Helicobacter pylori infection through multiple pathways.
abstract: Only a small percentage of people infected with Helicobacter pylori (H. pylori) will develop overt chronic gastric diseases. To understand the pathological mechanism, the action of H. pylori on monocyte apoptosis was detected. H. pylori co-culturing with peripheral blood monocytes, THP-1 or U937 cells result in early apoptosis at 6, 12, and 24 h after infection. The phosphorylated Bad and JNK were increased, and Bcl-2 was declined at 6, 12, and 24 h in peripheral blood monocytes after H. pylori infection. The phosphorylated Akt was augmented at 6 and 12 h post-infection. A slow apoptotic response was induced by H. pylori via Bad and Bcl-2 regulators, activated caspase-8 and caspase-9, and JNK at 24 h in THP-1 cells. Meanwhile, only Bad and JNK were involved in regulating U937 cells apoptosis at 24 h after infection. These results supported a novel mechanism of H. pylori escaping from monocytes by upregulation of early apoptosis and inhibition of late apoptosis. The differences among the three cells may reveal why H. pylori-derived disease occurs in relatively few people and provide a pathological mechanism whereby a treatment for H. pylori-derived disease may be developed.
authors.affiliation: School of Basic Medical Sciences, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Basic Medical Sciences, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Basic Medical Sciences, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Basic Medical Sciences, Binzhou Medical University, Yantai, 264003, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Basic Medical Sciences, Binzhou Medical University, Yantai, 264003, China. Electronic address: liboqing@bzmc.edu.cn.
authors.forename: Boqing
authors.initials: B
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D016481:Helicobacter Infections / Q000276:immunology / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000382:microbiology / Q000473:pathology*
keywords: Apoptosis; Death receptor; Helicobacter pylori; Mitochondrial; Monocytes