title: Assessment and Optimizations of Candida albicansIn VitroBiofilm Assays.
abstract: Candida albicansbiofilms have a significant medical impact due to their rapid growth on implanted medical devices, their resistance to antifungal drugs, and their ability to seed disseminated infections. Biofilm assays performedin vitroallow for rapid, high-throughput screening of gene deletion libraries or antifungal compounds and typically serve as precursors toin vivostudies. Here, we compile and discuss the protocols for several recently publishedC. albicansin vitrobiofilm assays. We also describe improved versions of these protocols as well as novelin vitroassays. Finally, we consider some of the advantages and disadvantages of these different types of assays.
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.|Department of Biology, BioSynesis, Inc., San Francisco, California, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Lohse
authors.affiliation: Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced, Merced, California, USA.
authors.forename: Megha
authors.initials: M
authors.lastname: Gulati
authors.affiliation: Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced, Merced, California, USA.|Quantitative and Systems Biology Graduate Program, University of California, Merced, Merced, California, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Valle Arevalo
authors.affiliation: Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced, Merced, California, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Fishburn
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.|Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Johnson
authors.affiliation: Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced, Merced, California, USA cnobile@ucmerced.edu.
authors.forename: Clarissa J
authors.initials: CJ
authors.lastname: Nobile
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000187:drug effects* / Q000472:pathogenicity*; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D000077336:Caspofungin; D002448:Cell Adhesion / Q000502:physiology*; D054714:Echinocandins / Q000494:pharmacology; D006801:Humans; D055666:Lipopeptides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D044085:Microfluidics / Q000379:methods*; D016459:Prosthesis-Related Infections / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D000077336:Caspofungin
keywords: Candida albicans; biofilm; biofilm methods; biofilm protocols; biofilm screens; in vitro biofilm assays