title: Plant Ethylene Detection Using Laser-Based Photo-Acoustic Spectroscopy.
abstract: Analytical detection of the plant hormone ethylene is an important prerequisite in physiological studies. Real-time and super sensitive detection of trace amounts of ethylene gas is possible using laser-based photo-acoustic spectroscopy. This Chapter will provide some background on the technique, compare it with conventional gas chromatography, and provide a detailed user-friendly hand-out on how to operate the machine and the software. In addition, this Chapter provides some tips and tricks for designing and performing physiological experiments suited for ethylene detection with laser-based photo-acoustic spectroscopy.
authors.affiliation: Faculty of Sciences, Laboratory of Functional Plant Biology, Department of Physiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium.
authors.forename: Bram
authors.initials: B
authors.lastname: Van de Poel
authors.affiliation: Faculty of Sciences, Laboratory of Functional Plant Biology, Department of Physiology, Ghent University, K.L. Ledeganckstraat 35, 9000, Gent, Belgium. dominique.vanderstraeten@ugent.be.
authors.forename: Dominique
authors.initials: D
authors.lastname: Van Der Straeten
mesh_terms: D002138:Calibration; D005030:Ethylenes / Q000032:analysis* / Q000378:metabolism; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D010937:Plant Growth Regulators / Q000032:analysis* / Q000378:metabolism; D012984:Software; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D013223:Statistics as Topic
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators
keywords: ETD-300; Ethylene; Laser-based photo-acoustic spectroscopy; Real-time measurements