title: Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes.
abstract: Exosomes are extracellular vesicles (50-100 nm) circulating in biofluids as intercellular signal transmitters. Although the potential of cancerous exosomes as tumor biomarkers is promising, sensitive and rapid detection of exosomes remains challenging. Herein, we combined the strengths of advanced aptamer technology, DNA-based nanostructure, and portable electrochemical devices to develop a nanotetrahedron (NTH)-assisted aptasensor for direct capture and detection of hepatocellular exosomes. The oriented immobilization of aptamers significantly improved the accessibility of an artificial nucleobase-containing aptamer to suspended exosomes, and the NTH-assisted aptasensor could detect exosomes with 100-fold higher sensitivity when compared to the single-stranded aptamer-functionalized aptasensor. The present study provides a proof-of-concept for sensitive and efficient quantification of tumor-derived exosomes. We thus expect the NTH-assisted electrochemical aptasensor to become a powerful tool for comprehensive exosome studies.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology , Beijing 100029, China.|Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Sai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.|Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Liqin
authors.identifier: 0000-0002-3632-4117
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wan
authors.affiliation: Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Sena
authors.initials: S
authors.lastname: Cansiz
authors.affiliation: Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cui
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.|Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Ren
authors.initials: R
authors.lastname: Cai
authors.affiliation: Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Hong
authors.affiliation: Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: I-Ting
authors.initials: IT
authors.lastname: Teng
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.|Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Muling
authors.initials: M
authors.lastname: Shi
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.|Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.|Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Weihong
authors.identifier: 0000-0002-8066-1524
authors.initials: W
authors.lastname: Tan
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D055354:Exosomes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis*; D010316:Particle Size; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D061925:Immobilized Nucleic Acids; D006046:Gold
keywords: aptasensor; electrochemistry; exosomes; expanded nucleotide; nanotetrahedron