title: Enzyme-antibody dual-film modified gold nanoparticle probe for ultrasensitive detection of alpha fetoprotein.
abstract: In this study, we designed a comprehensive strategy for the ultrasensitive detection of alpha fetoprotein (AFP) with high specificity using gold nanoparticle (AuNP)-based enzyme-linked immunosorbent assay (ELISA). A dual-film modified probe was synthesized by coating AuNPs with horseradish peroxidase (HRP) on its surface. Anti-AFP monoclonal antibody (McAb) was immobilized on the surface of the enzyme using glutaraldehyde cross-linking method. AuNPs, employed as support for the immobilization of HRP. HRP was used not only as the enzymatic-amplified tracer but also as a bridge for loading McAb. The limit of detection was 2 ng mL-1. The developed probes can provide an alternative approach with high sensitivity and a simple process similar to that of the traditional HRP-McAb based ELISA for the ultrasensitive detection of AFP in serum.
authors.affiliation: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, China.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, China.
authors.forename: Suiping
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, China. Electronic address: fengbo@xtu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000509:alpha-Fetoproteins; D006046:Gold; D006735:Horseradish Peroxidase
keywords: AFP; ELISA; Gold nanoparticle; Nanoprobe