title: Generation of trophoblast-like cells from the amnion in vitro: A novel cellular model for trophoblast development.
abstract: Despite the high incidence of trophoblast-related diseases, the molecular mechanism of inadequate early trophoblast development is still unclear due to the lack of an appropriate cellular model in vitro. In the present study, we reprogrammed the amniotic cells to be induced pluripotent stem cells (iPSCs) via a non-virus and non-integrated method and subsequently differentiated them into trophoblast-like cells by a modified BMP4 strategy in E6 medium. Compared with the previously studied trophoblast-like cells from ESCs, the iPSCs derived trophoblast-like cells behave similarly in terms of gene expression profiles and biofunctions. Also we confirmed the differentiating tendency from iPSCs to be syncytiotrophoblasts-like cells might be caused by inappropriate differentiating oxygen condition. Additionally, we preliminarily indicated in vitro "artificial" differentiation of iPSCs also undergoing a possible trophoblastic stem cell stage, as witnessed in vivo. In conclusion, we provided an in vitro cellular model to study early trophoblast development for specific individual, by using the feasible amnion.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510530, China. Electronic address: davidwei.smu@foxmail.com.
authors.forename: Yanxing
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510530, China. Electronic address: 1010199007@qq.com.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China. Electronic address: 695181799@qq.com.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Huang
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Affiliated Hospital of Hainan Medical College, Haikou, Hainan, 570102, China. Electronic address: 1071545806@qq.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Long
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510530, China. Electronic address: 939992238@qq.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Xiao
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China. Electronic address: 674200462@qq.com.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510530, China. Electronic address: zhongmei@smu.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China. Electronic address: pan_guangjin@gibh.ac.cn.
authors.forename: Guangjin
authors.initials: G
authors.lastname: Pan
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510530, China; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Affiliated Hospital of Hainan Medical College, Haikou, Hainan, 570102, China. Electronic address: mayl1990@foxmail.com.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510530, China. Electronic address: yuyh1010@hotmail.com.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000328:Adult; D000650:Amnion / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D005260:Female; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D059467:Transcriptome / Q000187:drug effects; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D055415:Bone Morphogenetic Protein 4
keywords: Amnion; BMP4; Differentiation; Induced pluripotent stem cell; Trophoblast