title: A comparative analysis of the physiological properties of neurons in the anterolateral bed nucleus of the stria terminalis in the Mus musculus, Rattus norvegicus, and Macaca mulatta.
abstract: The anterolateral group of the bed nucleus of the stria terminalis (BNSTALG) is a critical modulator of a variety of rodent and primate behaviors spanning anxiety behavior and drug addiction. Three distinct neuronal cell types have been previously defined in the rat BNSTALGbased on differences in the voltage-response to hyperpolarizing and depolarizing current injection. Differences in genetic expression profile between these three cell types suggest electrophysiological cell type may be an indicator for functional differences in the circuit of the rat BNSTALG. Although the behavioral role of the BNST is conserved across species, it is unknown if the same electrophysiological cell types exist in the BNSTALGof the mouse and nonhuman primate. Here, we used whole-cell patch clamp electrophysiology and neuronal reconstructions of biocytin-filled neurons to compare and contrast the electrophysiological and morphological properties of neurons in the BNSTALGfrom the mouse, rat, and rhesus macaque. We provide evidence that the BNSTALGof all three species contains neurons that match the three defined cell types found in the rat; however, there are intriguing differences in the relative frequency of these cell types as well as electrophysiological and morphological properties of the BNSTALGneurons across species. This study suggests that the overall landscape of the BNSTALGin the primate and mouse may be similar to that of the rat in some aspects but perhaps significantly different in others.
authors.affiliation: Behavioral Neuroscience and Psychiatric Disorders, Yerkes National Primate Research Center, Atlanta, Georgia.|Department of Psychiatry and Behavioral Science, Emory University School of Medicine, Atlanta, Georgia.
authors.forename: Sarah E
authors.identifier: http://orcid.org/0000-0003-3010-1120
authors.initials: SE
authors.lastname: Daniel
authors.affiliation: Behavioral Neuroscience and Psychiatric Disorders, Yerkes National Primate Research Center, Atlanta, Georgia.|Department of Psychiatry and Behavioral Science, Emory University School of Medicine, Atlanta, Georgia.
authors.forename: Jidong
authors.initials: J
authors.lastname: Guo
authors.affiliation: Behavioral Neuroscience and Psychiatric Disorders, Yerkes National Primate Research Center, Atlanta, Georgia.|Department of Psychiatry and Behavioral Science, Emory University School of Medicine, Atlanta, Georgia.
authors.forename: Donald G
authors.initials: DG
authors.lastname: Rainnie
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008239:Lysine / Q000031:analogs & derivatives; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000145:classification* / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D012686:Septal Nuclei / Q000166:cytology*; D013045:Species Specificity
chemical_list: C013411:biocytin; D008239:Lysine
keywords: RRID: AB_2315383; RRID: SCR_001622; RRID: SCR_001775; RRID: SCR_002798; extended amygdala; morphology; mouse; nonhuman primate; patch-clamp electrophysiology; rat