title: Pharmacological characterization of dopamine receptors in the rice striped stem borer, Chilo suppressalis.
abstract: Dopamine is an important neurotransmitter and neuromodulator in both vertebrates and invertebrates and is the most abundant monoamine present in the central nervous system of insects. A complement of functionally distinct dopamine receptors mediate the signal transduction of dopamine by modifying intracellular Ca2+and cAMP levels. In the present study, we pharmacologically characterized three types of dopamine receptors, CsDOP1, CsDOP2 and CsDOP3, from the rice striped stem borer, Chilo suppressalis. All three receptors show considerable sequence identity with orthologous dopamine receptors. The phylogenetic analysis also clusters the receptors within their respective groups. Transcript levels of CsDOP1, CsDOP2 and CsDOP3 were all expressed at high levels in the central nervous system, indicating their important roles in neural processes. After heterologous expression in HEK 293 cells, CsDOP1, CsDOP2 and CsDOP3 were dose-dependently activated by dopamine and synthetic dopamine receptor agonists. They can also be blocked by different series of antagonists. This study offers important information on three dopamine receptors from C. suppressalis that will provide the basis for forthcoming studies investigating their roles in behaviors and physiology, and facilitate the development of new insecticides for pest control.
authors.affiliation: State Key Laboratory of Rice Biology & Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: xugang0503@zju.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology & Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China; College of Plant Protection, Nanjing Agricultural University, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. Electronic address: wusf@njau.edu.cn.
authors.forename: Shun-Fan
authors.initials: SF
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Rice Biology & Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: gugx@zju.edu.cn.
authors.forename: Gui-Xiang
authors.initials: GX
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Rice Biology & Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: tzwbat@126.com.
authors.forename: Zi-Wen
authors.initials: ZW
authors.lastname: Teng
authors.affiliation: State Key Laboratory of Rice Biology & Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: chu@zju.edu.cn.
authors.forename: Gong-Yin
authors.initials: GY
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Rice Biology & Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: huangj@zju.edu.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018491:Dopamine Agonists / Q000737:chemistry; D018492:Dopamine Antagonists / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D011954:Receptors, Dopamine / Q000737:chemistry*; D017422:Sequence Analysis, DNA
chemical_list: D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D011954:Receptors, Dopamine
keywords: Chilo suppressalis; Dopamine receptor; Pharmacology; cAMP