title: Functional characterization of cytochrome P450 monooxygenases in the cereal head blight fungus Fusarium graminearum.
abstract: Fusarium graminearum is a prominent plant pathogenic fungus causing Fusarium head blight in major cereal crops worldwide. To understand the molecular mechanisms underlying fungal development and virulence, large collections of F. graminearum mutants have been constructed. Cytochrome P450 monooxygenases (P450s) are widely distributed in organisms and are involved in a diverse array of molecular/metabolic processes; however, no systematic functional analysis of P450s has been attempted in filamentous fungi. In this study, we constructed a genome-wide deletion mutant set covering 102 P450s and analyzed these mutants for changes in 38 phenotypic categories, including fungal development, stress responses and responses to several xenobiotics, to build a comprehensive phenotypic dataset. Most P450 mutants showing defective phenotypes were impaired in a single phenotypic trait, demonstrating that our mutant library is a good genetic resource for further fungal genetic studies. In particular, we identified novel P450s specifically involved in virulence (5) and both asexual (1) and sexual development (2). Most P450s seem to play redundant roles in the degradation of xenobiotics in F. graminearum. This study is the first phenome-based functional analysis of P450s, and it provides a valuable genetic resource for further basic and applied biological research in filamentous fungi and other plant pathogens.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Shin
authors.affiliation: Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Duc-Cuong
authors.initials: DC
authors.lastname: Bui
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Yoonji
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Nam
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Soyun
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Miao
authors.initials: M
authors.lastname: Fang
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186, Republic of Korea.
authors.forename: Jin-Cheol
authors.initials: JC
authors.lastname: Kim
authors.affiliation: Microbial Safety Team, National Academy of Agricultural Science, Rural Development Administration, Wanju, 55365, Republic of Korea.
authors.forename: Theresa
authors.initials: T
authors.lastname: Lee
authors.affiliation: Eco-friendly New Materials Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, 34114, Republic of Korea.
authors.forename: Hun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Eco-friendly New Materials Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, 34114, Republic of Korea.
authors.forename: Gyung Ja
authors.initials: GJ
authors.lastname: Choi
authors.affiliation: Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Hokyoung
authors.initials: H
authors.lastname: Son
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Yin-Won
authors.initials: YW
authors.lastname: Lee
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D002523:Edible Grain / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics*; D005670:Fusarium / Q000235:genetics* / Q000472:pathogenicity; D055786:Gene Knockout Techniques; D025282:Mycelium / Q000235:genetics / Q000254:growth & development; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology; D017384:Sequence Deletion; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development; D014908:Triticum / Q000382:microbiology; D014774:Virulence; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System