title: Inhibition of potassium currents is involved in antiarrhythmic effect of moderate ethanol on atrial fibrillation.
abstract: Excessive consumption of alcohol is a well-established risk factor of atrial fibrillation (AF). However, the effects of moderate alcohol drinking remain to be elucidated. This study was designed to determine the effects of moderate ethanol ingestion on atrial fibrillation and the electrophysiological mechanisms. In acetylcholine-induced canine and mouse AF models, the moderate ethanol prevented the generation and persistence of AF through prolonging the latent period of AF and shortening the duration of AF. The action potential duration (APD) was remarkably prolonged under the concentration range of 12.5-50.0mM ethanol in guinea pig atrial myocytes. Ultra-rapid delayed rectified potassium currents (IKv1.5) were markedly inhibited by 12.5-50.0mM ethanol in a concentration-dependent manner. Ethanol with 50.0mM could inhibit rapid delayed rectifier potassium currents (IhERG). Ethanol under 6.25-50.0mM did not affect on inward rectifier potassium currents (IKir2.1). Collectively, the present study provided an evidence that moderate ethanol intake can prolong the APD of atrial myocytes by inhibition of IKv1.5and IhERG, which contributed to preventing the development and duration of AF.
authors.affiliation: Department of Pharmacology, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Baode
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Chenxing
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Sun
authors.affiliation: Basic Medical Experiment Teaching Center, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Xinghui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Basic Medical Experiment Teaching Center, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Health Science Center, Xi'an Jiaotong University, Xi'an, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Health Science Center, Xi'an Jiaotong University, Xi'an, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, Health Science Center, Xi'an Jiaotong University, Xi'an, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, China. Electronic address: huhao@mail.xjtu.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000008:administration & dosage*; D001281:Atrial Fibrillation / Q000188:drug therapy* / Q000503:physiopathology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage*; D006168:Guinea Pigs; D057809:HEK293 Cells; D006801:Humans; D051666:Kv1.5 Potassium Channel / Q000037:antagonists & inhibitors* / Q000502:physiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D026902:Potassium Channel Blockers / Q000008:administration & dosage*
chemical_list: D000889:Anti-Arrhythmia Agents; C496981:KCNA5 protein, human; D051666:Kv1.5 Potassium Channel; D026902:Potassium Channel Blockers; D000431:Ethanol
keywords: Action potential duration; Atrial fibrillation; Ethanol; Ultra-rapid delayed rectifying potassium current