title: Hypothermic storage of isolated spermatogonia and oogonia from rainbow trout (Oncorhynchus mykiss).
abstract: A growing number of fish species are endangered due to human activities. A short- or long-time preservation of gametes could conserve genetic resources of threatened fish species. The aim of this study was to evaluate a hypothermic condition for short-term preservation of spermatogonia and oogonia cells isolated from immature transgenic rainbow trout, Oncorhynchus mykiss, and to determine the maximum time point for further transplantation. Viability rate of germ cells was investigated after isolation and during storage at 4 °C up to 24 h. Dulbecco's modification of Eagle's medium supplemented with Hepes fetal bovine serum and l-glutamine was used as hypothermic storage media. The results showed that while viability decreased following 24 h storage, the remaining viable cells did not vary morphologically as well as GFP intensity retained similar to those observed in freshly isolated cells. The hypothermal storage study indicated that culture medium is suitable for preserving germ cells in the short periods of time. Simplicity, easily available culture media and low cost provide new insight into hypothermic conditions for preserving and transporting of germ cells for next applied and basic studies.
authors.affiliation: Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran. Electronic address: falahatkar@guilan.ac.ir.
authors.forename: Bahram
authors.initials: B
authors.lastname: Falahatkar
authors.affiliation: Fisheries Department, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Iran. Electronic address: s.poursaeid@yahoo.com.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Poursaeid
authors.affiliation: Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Kitada
authors.affiliation: Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Yoshizaki
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D003080:Cold Temperature; D003470:Culture Media; D005260:Female; D005973:Glutamine; D008297:Male; D017686:Oncorhynchus mykiss; D009867:Oogonia; D044967:Serum; D013093:Spermatogonia; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D003470:Culture Media; D005973:Glutamine
keywords: Breeding program; Cold storage; Germ cells; Salmonids