title: Developmental studies on two ecdysone deficient mutants ofDrosophila melanogaster.
abstract: This paper describes two ecdysone-deficient, recessive-lethal mutants,lethal(1)giant ring gland (grg) andlethal(1)suppressor of forkedmad-ts(mad-ts: Jürgens and Gateff 1979) and compares their ecdysteroid titers with that of the wild-type. Mutant larvae show a much reduced ecdysteroid content, amounting to 1/10 to 1/30 of the wild-type values, but never a true titer peak. They fail to pupate and die after 1-3 weeks. Ecdysteroid feeding elicits different responses in the larvae of the two mutants.mad-ts larvae pupate within 24 h, thus showing that their low ecdysteroid titer is directly connected to their inability to pupate.mad-ts resembles the mutantlethal (3)ecdysone-1ts(Garen et al. 1977). Thegrg mutant larvae, on the other hand, fail to pupate after 20-hydroxyecdysone feeding as well as injection. The primary defect of thegrg mutant is not entirely clear. Thegrg larval salivary gland cells appear to possess normal ecdysteroid receptors. Furthermore, the low ecdysteroid titer ingrg is not the result of an increased ecdysteroid catabolism. The primary defect in the mutant may lie in the malfunctioning neurosecretory cells which do not show neurosecretion in histological preparations. Further support for this notion comes from electronmicrographs of the enlargedgrg ring glands which, in contrast to the wild-type, do not possess nerve endings.In the wild-type three ecdysteroid peaks were found: one shortly before puparium formation, the second at approximately 12 h and the third at about 30 h after pupation. The ecdysteroid titer peak in late third instar, wild-type larvae is mainly due to the presence of 20-dydroxyecdysone as shown by radioimmunoassays after thin layer chromatography and derivatization followed by gas liquid chromatography and mass spectroscopy. In addition, a number of unidentified polar and apolar metabolites were also present.
authors.affiliation: Institut für Zoologie, Technische Hochschule Darmstadt, Schnittspahnstr. 3, D-6100, Darmstadt.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Klose
authors.affiliation: Biologisches Institut I (Zoologie), Albert-Ludwigs Universität, Schänzlestr. 1, D-7800, Freiburg i. Br.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Gateff
authors.affiliation: Institut für Zoologie, Technische Hochschule Darmstadt, Schnittspahnstr. 3, D-6100, Darmstadt.
authors.forename: Hans
authors.initials: H
authors.lastname: Emmerich
authors.affiliation: Zentrallabor für Mutagenitätsforschung der Deutschen Forschungsgemeinschaft, Breisacherstr. 33, D-7800, Freiburg i.Br., Federal Republic of Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Beikirch
keywords: Drosophila; Ecdysone deficient mutants; Ecdysteroid titer; Fine structure; Ring gland