title: Platynosomum fastosum(Trematoda: Dicrocoeliidae) from Cats in Vietnam: Morphological Redescription and Molecular Phylogenetics.
abstract: The present study was performed to reveal the morphological characteristics and molecular phylogenetic position ofPlatynosomum fastosumKossack, 1910. A total 167 specimens ofP. fastosumwere collected in 8 (4.9%) out of 163 sets of gall-bladders and bile ducts of cats. The number of worms was 1-105 per infected cat. This species was characterized by having a long and slender body, slightly larger ventral sucker than the oral sucker, indistinct prepharynx, small pharynx, short esophagus, bifurcation midway between 2 suckers, and ceca extending to the posterior end of the body. The length of the partial sequences of ITS1 and 5.8S rDNA ofP. fastosumwere 990 bp, GC-rich. AT/GC ratio was 0.9, there were 9 polymorphic sites, and intraspecific variations ranged from 0.1% to 0.9%. Phylogenetic analyses by neighbor-joining phylogram inferred from ITS1 rDNA sequences revealed that the genetic distance betweenP. fastosumspecimens ranged from 0.3 to 1.5% while the smallest interspecific distance among dicrocoeliid species was 20.9 %. The redescription and genetic characters ofP. fastosumare taxonomically important to recognize future different species of the genusPlatynosomumshowing high intraspecific and morphological variability.
authors.affiliation: Department of Parasitology, Institute of Ecology and Biological Resources (IEBR), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
authors.forename: Hung Manh
authors.initials: HM
authors.lastname: Nguyen
authors.affiliation: Department of Parasitology, Institute of Ecology and Biological Resources (IEBR), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
authors.forename: Hien
authors.initials: H
authors.lastname: Van Hoang
authors.affiliation: Department of Molecular Systematics and Conservation Genetics, Institute of Ecology and Biological Resources (IEBR), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
authors.forename: Loan Thi
authors.initials: LT
authors.lastname: Ho
mesh_terms: D000818:Animals; D001482:Base Composition; D001652:Bile Ducts / Q000469:parasitology; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004012:Dicrocoeliidae / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D005704:Gallbladder / Q000469:parasitology; D008853:Microscopy; D010802:Phylogeny; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*; D014744:Vietnam
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S
keywords: Platynosomum fastosum; dicrocoeliid; hepatic trematode; morphometry; nuclear ribosomal DNA; phylogenetics