title: Phase II Conjugates of Urolithins Isolated from Human Urine and Potential Role ofβ-Glucuronidases in Their Disposition.
abstract: In recent years, many xenobiotics derived from natural products have been shown to undergo extensive metabolism by gut microbiota. Ellagitannins, which are high molecular polyphenols, are metabolized to dibenzo[b,d]pyran-6-one derivatives-urolithins. These compounds, in contrast with their parental compounds, have good bioavailability and are found in plasma and urine at micromolar concentrations. In vivo studies conducted for ellagitannin-containing natural products indicate their beneficial health effects toward inflammation and cancer, which are associated with the formation of urolithins. However, the great majority of in vitro experiments that have revealed the molecular mechanisms responsible for the observed effects were conducted for urolithin aglycones. These studies are thus incongruent with the results of pharmacokinetic studies that clearly indicate that glucuronide conjugates are the dominant metabolites present in plasma, tissue, and urine. The aim of this study was to isolate and structurally characterize urolithin conjugates from the urine of a volunteer who ingested ellagitannin-rich natural products, and to evaluate the potential role ofβ-glucuronidase-triggered cleavage in urolithin disposition. Glucuronides of urolithin A, iso-urolithin A, and urolithin B were isolated and shown to be cleaved by theβ-glucuronidases released by neutrophils from azurophilic granules uponN-formylmethionine-leucyl-phenylalanine stimulation as well as byEscherichia colistandard strains and clinical isolates from patients with urinary tract infections. These results justify the hypothesis that the selective activation of urolithin glucuronides byβ-glucuronidase, which are present at high concentrations at inflammation and infection sites and in the microenvironments of solid tumors, could locally increase the concentration of bioactive urolithin aglycones.
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy (J.P.P., I.S., S.G., A.K.K.), and Department of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT) (J.S.), Medical University of Warsaw, Warsaw, Poland; Primary Laboratory of Origin: Medical University of Warsaw, Faculty of Pharmacy jakub.piwowarski@wum.edu.pl.
authors.forename: Jakub P
authors.initials: JP
authors.lastname: Piwowarski
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy (J.P.P., I.S., S.G., A.K.K.), and Department of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT) (J.S.), Medical University of Warsaw, Warsaw, Poland; Primary Laboratory of Origin: Medical University of Warsaw, Faculty of Pharmacy.
authors.forename: Iwona
authors.initials: I
authors.lastname: Stanisławska
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy (J.P.P., I.S., S.G., A.K.K.), and Department of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT) (J.S.), Medical University of Warsaw, Warsaw, Poland; Primary Laboratory of Origin: Medical University of Warsaw, Faculty of Pharmacy.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Granica
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy (J.P.P., I.S., S.G., A.K.K.), and Department of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT) (J.S.), Medical University of Warsaw, Warsaw, Poland; Primary Laboratory of Origin: Medical University of Warsaw, Faculty of Pharmacy.
authors.forename: Joanna
authors.initials: J
authors.lastname: Stefańska
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy (J.P.P., I.S., S.G., A.K.K.), and Department of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT) (J.S.), Medical University of Warsaw, Warsaw, Poland; Primary Laboratory of Origin: Medical University of Warsaw, Faculty of Pharmacy.
authors.forename: Anna K
authors.initials: AK
authors.lastname: Kiss
mesh_terms: D000328:Adult; D001688:Biological Products / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism* / Q000652:urine*; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000378:metabolism; D008297:Male; D010949:Plasma / Q000378:metabolism; D014552:Urinary Tract Infections / Q000378:metabolism; D014556:Urine / Q000737:chemistry*
chemical_list: D001688:Biological Products; D003374:Coumarins; D047348:Hydrolyzable Tannins; C000595064:urolithin B; C026423:3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one; D005966:Glucuronidase