title: Pouteria torta epicarp as a useful source of α-amylase inhibitor in the control of type 2 diabetes.
abstract: Type 2 diabetes plays a major role in public health, affecting about 400 million adults. One of the used strategies to control type 2 diabetes is the inhibition of α-amylase activity to reduce post-prandial blood glucose levels. Therefore, in past decades, the search of new α-amylase inhibitors has led to the evaluation of natural products as a source of these compounds. Pouteria torta (Sapotaceae) is widespread in Brazil and bears edible fruits. Epicarp and pulp crude extracts of fresh fruits were studied for in vitro α-amylase inhibition activity. The pulp did not present activity while epicarp, usually considered as waste, showed a high α-amylase inhibitory capacity when compared with acarbose and Triticum aestivum. Therefore, an assay-guided fractionation study of epicarp crude extract was performed. Fraction VI shows very high inhibitory activity with IC50of 9 μg/mL. However, subsequent fractionation led to lower inhibition potential (IC50of 22.1 μg/mL). The qualitative characterization of fraction VI were performed by chromatographic and spectrometric analysis and showed the presence of epicatechin, catechin, sucrose, glucose, and fructose. Total phenolic and flavonoid contents and antioxidant capacity were also assessed and there seemed to be no correlation between phenolic or flavonoids-rich fractions and antioxidant capacity or α-amylase inhibitory activity.
authors.affiliation: Laboratório Central do Distrito Federal/LACEN, Secretaria de Saúde do Distrito Federal, Lotes O e P, Sgan 601, Asa Norte, CEP 70830-010 Brasília, Brazil.
authors.forename: Paloma Michelle
authors.initials: PM
authors.lastname: de Sales
authors.affiliation: Laboratorio de Produtos Naturais, Faculdade de Ciências da Saúde, Universidade de Brasília, Asa Norte, CEP 70910-900 Brasília, Brazil.
authors.forename: Paula Monteiro
authors.initials: PM
authors.lastname: de Souza
authors.affiliation: Laboratorio de Produtos Naturais, Faculdade de Ciências da Saúde, Universidade de Brasília, Asa Norte, CEP 70910-900 Brasília, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Dartora
authors.affiliation: Instituto de Química, Universidade de Brasília, Asa Norte, CEP 70910-900, Brasília, Brazil.
authors.forename: Inês Sabioni
authors.initials: IS
authors.lastname: Resck
authors.affiliation: Laboratorio de Produtos Naturais, Faculdade de Ciências da Saúde, Universidade de Brasília, Asa Norte, CEP 70910-900 Brasília, Brazil.
authors.forename: Luiz Alberto
authors.initials: LA
authors.lastname: Simeoni
authors.affiliation: Laboratorio de Produtos Naturais, Faculdade de Ciências da Saúde, Universidade de Brasília, Asa Norte, CEP 70910-900 Brasília, Brazil.
authors.forename: Yris Maria
authors.initials: YM
authors.lastname: Fonseca-Bazzo
authors.affiliation: Laboratorio de Produtos Naturais, Faculdade de Ciências da Saúde, Universidade de Brasília, Asa Norte, CEP 70910-900 Brasília, Brazil.
authors.forename: Pérola
authors.initials: P
authors.lastname: de Oliveira Magalhães
authors.affiliation: Laboratorio de Produtos Naturais, Faculdade de Ciências da Saúde, Universidade de Brasília, Asa Norte, CEP 70910-900 Brasília, Brazil. Electronic address: damaris@unb.br.
authors.forename: Dâmaris
authors.initials: D
authors.lastname: Silveira
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D001938:Brazil; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D005638:Fruit; D006801:Humans; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D032132:Pouteria / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases
keywords: Agroindustrial waste; Diabetes; Pouteria torta; Sapotaceae; α-amylase inhibitor