title: Development of a novel ultrasound-assisted alkali pretreatment strategy for the production of bioethanol and xylanases from chili post harvest residue.
abstract: A novel ultrasound-assisted alkali pretreatment strategy was developed which could effectively remove lignin and hemicelluloses and improve the sugar yield from chili post harvest residue. Operational parameters that affect the pretreatment efficiency were studied and optimized. Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, 5-hydroxymethyl furfural as well as organic acids like citric acid, succinic acid and propionic acid were absent. Hence fermentation can be carried out without detoxification of the hydrolyzate. Changes in structural properties of the biomass were studied in relation to the pretreatment process using Scanning Electron Microscopy (SEM) and the changes in chemical composition were also monitored. The biomass pretreated with the optimized novel method could yield 0.428g/g of reducing sugars upon enzymatic hydrolysis. The hydrolyzate obtained by this novel pretreatment strategy was found to be suitable for bioethanol and xylanase production.
authors.affiliation: Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India. Electronic address: sindhurgcb@gmail.com.
authors.forename: Raveendran
authors.initials: R
authors.lastname: Sindhu
authors.affiliation: Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India.
authors.forename: Parameswaran
authors.initials: P
authors.lastname: Binod
authors.affiliation: Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India.
authors.forename: Anil Kuruvilla
authors.initials: AK
authors.lastname: Mathew
authors.affiliation: Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India.
authors.forename: Amith
authors.initials: A
authors.lastname: Abraham
authors.affiliation: Ecole Polytechnique Federale de Lausanne, Institute of Urban and Regional Sciences, GC A3, Station 18, CH-1015 Lausanne, Switzerland.
authors.forename: Edgard
authors.initials: E
authors.lastname: Gnansounou
authors.affiliation: Institute of Bioresources and Sustainable Development (IBSD), Imphal 795001, Manipur, India.
authors.forename: Sabeela Beevi
authors.initials: SB
authors.lastname: Ummalyma
authors.affiliation: Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India.
authors.forename: Leya
authors.initials: L
authors.lastname: Thomas
authors.affiliation: Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India; Center of Innovative and Applied Bioprocessing, C-127, II Floor, Phase 8, Industrial Area, SAS Nagar, Mohali 160 071, Punjab, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
mesh_terms: D000468:Alkalies; D000431:Ethanol; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin
chemical_list: D000468:Alkalies; D000431:Ethanol; D008031:Lignin
keywords: Bioethanol; Chili; Pretreatment; Saccharification; Xylanases