title: Controlled release of insulin from folic acid-insulin complex nanoparticles.
abstract: Associative interactions between folic acid and proteins are well known. This work leverages these interactions to engineer folic acid nanoparticles for controlled release of insulin during diabetes therapy. The insulin-loaded folic acid nanoformulation is synthesized during this study to achieve better insulin loading and encapsulation than previous strategies. The maximum insulin loading in the FA particles was kept at 6mg with less than 10% insulin loss during the synthesis process which is significantly better compare to previous strategies. The folic acid nanoparticles of 50-150nm size are further characterized in the present study. The release behaviour of insulin from the nanoparticles has been studied to quantify released insulin and folic acid with time using high performance liquid chromatography. Insulin release results suggest that more than 90% of the insulin is encapsulated and released within 24h from folic acid nanoparticles. The analysis of folic acid release along with insulin release indicates that the particles are formed by folic acid-insulin complexation at the molecular level. The release of insulin from nanoparticles is controllable with the change in the crosslinking salt concentration as well as the amount of folic acid loaded during particle synthesis. These results prove that folic acid nanocarriers are capable to control the release of therapeutic proteins.
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Delhi, New Delhi, 110016, India.
authors.forename: Rajat
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Delhi, New Delhi, 110016, India. Electronic address: sanat.mohanty@gmail.com.
authors.forename: Sanat
authors.initials: S
authors.lastname: Mohanty
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations / Q000737:chemistry; D003920:Diabetes Mellitus / Q000188:drug therapy / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D005492:Folic Acid / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D010316:Particle Size
chemical_list: D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D007328:Insulin; D053769:Nanocapsules; D005492:Folic Acid
keywords: Controlled release; Folic acid; HPLC; Insulin delivery; Nanoparticle