title: A quantitative analysis of multilineage-differentiating stress-enduring (Muse) cells in human adipose tissue and efficacy of melanocytes induction.
abstract: We have shown previously that multilineage-differentiating stress-enduring (Muse) cells in neonatal fibroblasts can differentiate into functional melanocytes. In this study, we quantitate Muse cells in adipose-mesenchymal stem cells (adipose-MSCs) of human subcutaneous tissue obtained from 11 subjects of various ages, and measured efficacy of melanocytes induction from Adipose-MSC-derived Muse cells (hASC-Muse cells). There was a statistically significant negative correlation between the age of donors and the numbers of adipose-MSCs recovered per g fat as well as the percentage of SSEA3+ cells in the adipose-MSC populations, but isolated hASC-Muse cells showed pluripotency and growth curves equally regardless the age of donors. Adipose-Muse cells sequentially expressed melanocyte-related genes including KIT, MITF, TYRP1 PMEL, DCT, melanocortin 1 receptor (MC1R), and TYR at a comparable level to melanocytes during 6-week culture. Parallel with MC1R expression, adipose-Muse cells increased melanin content by α-MSH stimulation. By quantitating the cell numbers recovered at each step, we found that 10g of adipose tissue could produce at least 2.5×106melanocytes after 6 weeks of culture. These studies suggest that induction of melanocytes from adipose-Muse is a novel approach to obtain sufficient numbers of melanocytes for clinical application and in vitro study of melanocyte differentiation.
authors.affiliation: Department of Dermatology, Tohoku University Graduate School of Medicine, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamauchi
authors.affiliation: Department of Dermatology, Tohoku University Graduate School of Medicine, Japan. Electronic address: kyamasaki@med.tohoku.ac.jp.
authors.forename: Kenshi
authors.initials: K
authors.lastname: Yamasaki
authors.affiliation: Department of Dermatology, Tohoku University Graduate School of Medicine, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Tsuchiyama
authors.affiliation: Department of Dermatology, Tohoku University Graduate School of Medicine, Japan.
authors.forename: Saaya
authors.initials: S
authors.lastname: Koike
authors.affiliation: Department of Dermatology, Tohoku University Graduate School of Medicine, Japan.
authors.forename: Setsuya
authors.initials: S
authors.lastname: Aiba
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D006801:Humans; D008024:Ligands; D008543:Melanins / Q000378:metabolism*; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000187:drug effects* / Q000378:metabolism; D044102:Receptor, Melanocortin, Type 1 / Q000819:agonists / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D050151:Subcutaneous Fat / Q000166:cytology*; D013997:Time Factors; D000521:alpha-MSH / Q000494:pharmacology*
chemical_list: D008024:Ligands; D008543:Melanins; D044102:Receptor, Melanocortin, Type 1; D000521:alpha-MSH