title: A new LC-MS/MS bioanalytical method for atenolol in human plasma and milk.
abstract: OBJECTIVE
  A new sensitive LC-MS/MS method for the quantification of atenolol in human plasma and milk has been developed for clinical lactation studies.
  
  
  RESULTS
  Atenolol and the internal standard, phenazone, were extracted from biological matrices by protein precipitation. A Phenomenex®C-18 column and gradient chromatographic conditions were used for separation of the analyte, followed by detection with MS. Stability of samples was confirmed for atenolol in human plasma and milk for up to 3 months. Linearity range of 1-800 ng/ml (r2= 0.9995), the precision within 15% CV and the recovery of the analyte (80-100% range) were achieved.
  
  
  CONCLUSIONS
  A new validated analytical method for atenolol in plasma and milk was developed.
authors.affiliation: School of Pharmacy & Medical Sciences, Reid Building, City East Campus, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Ei Mon
authors.initials: EM
authors.lastname: Phyo Lwin
authors.affiliation: School of Pharmacy & Medical Sciences, Reid Building, City East Campus, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Cobus
authors.initials: C
authors.lastname: Gerber
authors.affiliation: School of Pharmacy & Medical Sciences, Reid Building, City East Campus, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Song
authors.affiliation: Women's & Children's Hospital, SA Pharmacy, Adelaide, SA 5006, Australia.
authors.forename: Catherine
authors.initials: C
authors.lastname: Leggett
authors.affiliation: Women's & Children's Hospital, SA Pharmacy, Adelaide, SA 5006, Australia.
authors.forename: Usha
authors.initials: U
authors.lastname: Ritchie
authors.affiliation: Women's & Children's Hospital, SA Pharmacy, Adelaide, SA 5006, Australia.
authors.forename: Sean
authors.initials: S
authors.lastname: Turner
authors.affiliation: School of Pharmacy & Medical Sciences, Reid Building, City East Campus, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Garg
mesh_terms: D000818:Animals; D001262:Atenolol / Q000032:analysis*; D002138:Calibration; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008892:Milk / Q000378:metabolism*; D010949:Plasma / Q000378:metabolism*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001262:Atenolol
keywords: LC–MS/MS; atenolol; lactation; milk; plasma