title: Clinicopathological and prognostic significance of aberrant Arpin expression in gastric cancer.
abstract: OBJECTIVE
  To detect the expression of Arpin, and determine its correlation with clinicopathological characteristics and the prognosis of gastric cancer (GC) patients.
  
  
  METHODS
  A total of 176 GC patients were enrolled as study subjects and classified into groups according to different clinicopathological variables. GC mucosal tissues were obtainedviasurgery. Another 43 paraffin-embedded tissue blocks of normal gastric epithelium (> 5 cm away from the edge of the tumor) were included in the control group. Immunohistochemistry (IHC) for the Arpin and Arp3 proteins was performed on the formalin-fixed, paraffin-embedded GC tissues. Additionally, expression of the Arpin protein in 43 normal gastric tissues was also determined using IHC.
  
  
  RESULTS
  Expression of the Arpin protein in GC was lower than that in normal gastric mucosa (30.68%vs60.47%,P< 0.001). Aχ2test of the 176 GC samples used for IHC showed that decreased Arpin expression was associated with advanced TNM stage (P< 0.01) and the presence or absence of lymph node metastasis (80.92%vs35.56%,P< 0.001). Additionally, a significant correlation was observed between the expression of Arpin and the presence of the Arp2/3 complex in GC tissues (χ2= 30.535,P< 0.001). Moreover, a multivariate Cox regression analysis revealed that Arpin expression [hazard ratio (HR) = 0.551,P= 0.029] and TNM stage (HR = 5.344,P= 0.001) were independent prognostic markers for overall survival of GC patients. Regarding the 3-year disease-free survival (DFS), the recurrence rate of GC patients with low Arpin expression levels (median DFS 19 mo) was higher than that in the high-Arpin-expression group (median DFS 34 mo,P= 0.022).
  
  
  CONCLUSIONS
  Low Arpin levels are associated with clinicopathological variables and a poor prognosis in GC patients. Arpin may be regarded as a potential prognostic indicator in GC.
authors.affiliation: Tao Li, Hong-Mei Zheng, Ying-Jian Jiang, Jiang Wang, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Tao Li, Hong-Mei Zheng, Ying-Jian Jiang, Jiang Wang, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zheng
authors.affiliation: Tao Li, Hong-Mei Zheng, Ying-Jian Jiang, Jiang Wang, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Nai-Mei
authors.initials: NM
authors.lastname: Deng
authors.affiliation: Tao Li, Hong-Mei Zheng, Ying-Jian Jiang, Jiang Wang, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Ying-Jian
authors.initials: YJ
authors.lastname: Jiang
authors.affiliation: Tao Li, Hong-Mei Zheng, Ying-Jian Jiang, Jiang Wang, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Tao Li, Hong-Mei Zheng, Ying-Jian Jiang, Jiang Wang, Dian-Liang Zhang, Center of Colon and Rectum, Qingdao Municipal Hospital, Qingdao University, Qingdao 266011, Shandong Province, China.
authors.forename: Dian-Liang
authors.initials: DL
authors.lastname: Zhang
mesh_terms: D051378:Actin-Related Protein 3 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D015331:Cohort Studies; D018572:Disease-Free Survival; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism*
chemical_list: C495928:ACTR3 protein, human; D051378:Actin-Related Protein 3; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; C000604273:arpin protein, human
keywords: Arp2/3 complex; Arpin; Clinicopathological characteristics; Gastric cancer; Prognosis