title: Circular Dichroism and Fluorescence Spectroscopy to Study Protein Structure and Protein-Protein Interactions in Ethylene Signaling.
abstract: Circular dichroism (CD) spectroscopy is an invaluable technique to analyze secondary structure and functional folding of recombinant purified proteins. CD spectroscopy can also be applied to detect changes in protein secondary structure related to the pH or redox conditions found in different cellular compartments or to the interaction with other molecules. Another biophysical technique to monitor conformational changes and interaction with small molecule ligands or biological macromolecules is protein fluorescence spectroscopy making use of the aromatic amino acid tryptophan as a sensitive intrinsic fluorescent probe. Here, we describe the application of CD and tryptophan fluorescence spectroscopy to study soluble and membrane proteins of the ethylene signaling pathway.
authors.affiliation: Institute of Biochemical Plant Physiology, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, D-40225, Düsseldorf, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Kessenbrock
authors.affiliation: Institute of Biochemical Plant Physiology, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, D-40225, Düsseldorf, Germany. georg.groth@hhu.de.
authors.forename: Georg
authors.initials: G
authors.lastname: Groth
mesh_terms: D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism / Q000295:instrumentation / Q000379:methods; D004268:DNA-Binding Proteins; D005030:Ethylenes / Q000737:chemistry* / Q000378:metabolism*; D008024:Ligands; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C106987:EIN3 protein, Arabidopsis; C494590:ETR1 protein, Arabidopsis; D005030:Ethylenes; D008024:Ligands; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D014364:Tryptophan
keywords: Circular dichroism; Conformational change; Fluorescence spectroscopy; Membrane protein; Protein interactions; Protein stability; Protein structure; Small molecule ligand; Soluble protein; Tryptophan