Identification of microorganisms in biofluids of individuals with periodontitis and chronic kidney disease using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Chronic kidney disease (CKD) and periodontitis (PD) are important health issues. There is a large variety of microorganisms related to the pathogenesis of periodontitis, and optimising the time and the cost of laboratory assays to detect these organisms is highly valuable in the medical field.



Bacteria were isolated from saliva and oral biofilm of 30 adolescents and young adults with definite medical and dental diagnosis of CKD and PD, respectively, and proteins were extracted for microorganism identification by means of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) technique.



The results showed that the most incident microorganisms were Actinomyces dentalis (43%), Acinetobacter ursingi (60%), Aggregatibacter actinomycetencomitans (60%), Corynebacterium argentoctens (63%), Staphylococcus aureus (93%), Streptococcus salivarius (97%) and Tannerella forsythensis (43%). The analysis of oral biofilm showed higher incidences for Actinomyces dentalis (33%), Acinetobacter ursingi (50%), Aggregatibacter actinomycetencomitans (50%), Corynebacterium argentoctens (70%), Pseudomonas aeruginosa (40%), Staphylococcus aureus (73%) and Streptococcus salivarius (87%).



Based on these results, we concluded that the MALDI Biotyper protocol proves useful as a rapid and reliable assay for distinguishing different microorganisms possibly related to CKD and PD. Copyright © 2016 John Wiley & Sons, Ltd.
Orthodontics and Paediatric Department, Dental School, University of São Paulo (USP), Brazil.
Levy Anderson César
LA
Alves
Orthodontics and Paediatric Department, Dental School, University of São Paulo (USP), Brazil.
Rafael Celestino
RC
Souza
Orthodontics and Paediatric Department, Dental School, University of São Paulo (USP), Brazil.
Taciana Mara Couto
TM
da Silva
Paediatric Nephrology Department, University of São Paulo (USP), Brazil.
Andreia
A
Watanabe
LSCP - Chemical Engineering Department, University of São Paulo (USP), Brazil.
Meriellen
M
Dias
LSCP - Chemical Engineering Department, University of São Paulo (USP), Brazil.
Maria Anita
MA
Mendes
Orthodontics and Paediatric Department, Dental School, University of São Paulo (USP), Brazil.
Ana Lídia
AL
Ciamponi
D001419:Bacteria / Q000302:isolation & purification*; D006801:Humans; D010518:Periodontitis / Q000382:microbiology*; D051436:Renal Insufficiency, Chronic / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*