Protein Array-Based Detection of Proteins in Kidney Tissues from Patients with Membranous Nephropathy.
Membranous nephropathy (MN) is an autoimmune inflammatory disease in which proteins related with plenty of biological processes play an important role. However, the role of these proteins in the pathogenesis of MN is still unclear. This study aimed to screen differential proteins in kidney tissue samples from MN patients by using protein arrays and determine the pathways involved in the pathogenesis of MN. This study first tested a quantitative protein array (QAH-INF-3) and two semiquantitative protein arrays (L-493 and L-507) with normal renal tissue and identified L-493 as the most appropriate assay to compare protein levels between MN tissues and normal control tissues. The L-493 array identified 66 differentially expressed proteins (DEPs) that may be associated with MN. The gene oncology (GO) and protein-protein interaction (PPI) analyses revealed several processes potentially involved in MN, including extracellular matrix disassembly and organization, cell adhesion, cell-cell signaling, cellular protein metabolic process, and immune response (P< 0.05). We suggest that these different pathways work together via protein signaling and result in the pathogenesis and progression of MN.
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Shuqiang
0000-0002-4264-781X
S
Wang
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Yang
Y
Lu
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Quan
0000-0002-6839-7695
Q
Hong
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Xiaodong
0000-0003-1273-4979
X
Geng
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Xu
0000-0003-4254-4244
X
Wang
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Wei
0000-0002-2415-0594
W
Zheng
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Chengcheng
0000-0002-7370-6165
C
Song
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Chunling
0000-0003-2974-628X
C
Liu
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Meng
M
Fan
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Yue
0000-0002-1453-2716
Y
Xi
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Mandi
0000-0001-9996-5416
M
Guo
Department of Nephrology, PLA General Hospital, Institute of Nephrology, Beijing Key Laboratory of Kidney Disease, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Di
0000-0001-8490-3722
D
Wu
D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D063990:Gene Ontology; D015433:Glomerulonephritis, Membranous / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D008297:Male; D040081:Protein Array Analysis / Q000379:methods*; D060066:Protein Interaction Maps / Q000235:genetics*