A Combination of Soybean and Haematococcus Extract Alleviates Ultraviolet B-Induced Photoaging.
Soybean-derived isoflavones have been investigated for their preventative effects against UV-induced symptoms of skin damage including wrinkle formation and inflammation. Haematococcus pluvialis is a freshwater species of Chlorophyta that contains high concentrations of the natural carotenoid pigment astaxanthin. Astaxanthin is known to be involved in retinoic acid receptor (RAR) signaling and previously been associated with the inhibition of activator protein (AP)-1 dependent transcription. Based on previous studies, we hypothesized that a combination of soy extract (SE) and Haematococcus extract (HE) may prevent UVB-induced photoaging through specific signaling pathways, as measured by UVB-induced wrinkling on hairless mice skin and expression changes in human dermal fibroblasts (HDFs). The 1:2 ratio of SE and HE mixture (SHM) showed the optimal benefit in vivo. SHM was found to inhibit wrinkle formation via the downregulation of matrix metalloproteinase (MMP)-1 mRNA and protein expression. SHM also inhibited mitogen-activated protein kinase (MAPK) phosphorylation and the transactivation of AP-1 which plays an important role in regulating MMP expression. These results highlight the potential for SHM to be developed as a therapeutic agent to prevent UVB-induced skin wrinkling.
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea. 123456wldms@hanmail.net.
Jieun
J
Shin
Research Institute of Biotechnology and Medical Converged Science, Dongguk University (Seoul),Goyang 10326, Korea. kjparka@pulmuone.com.
Jong-Eun
JE
Kim
The Food and Culture Institute, Pulmuone Co., Ltd., Seoul 03722, Korea. jikang@pulmuone.com.
Kum-Ju
KJ
Pak
The Food and Culture Institute, Pulmuone Co., Ltd., Seoul 03722, Korea. tskim@pulmuone.com.
Jung Il
JI
Kang
The Food and Culture Institute, Pulmuone Co., Ltd., Seoul 03722, Korea. sylee@pulmuone.com.
Tae-Seok
TS
Kim
The Food and Culture Institute, Pulmuone Co., Ltd., Seoul 03722, Korea. hyeo@pulmuone.com.
Sang-Yoon
SY
Lee
The Food and Culture Institute, Pulmuone Co., Ltd., Seoul 03722, Korea. kiwon@snu.ac.kr.
Ik-Hyun
IH
Yeo
Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea. jyoon@hallym.ac.kr.
Jung Han Yoon
JH
Park
Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea. killrose@hotmail.com.
Jong Hun
JH
Kim
School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea. njkang@knu.ac.kr.
Nam Joo
NJ
Kang
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea. kiwon@snu.ac.kr.|Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea. kiwon@snu.ac.kr.
Ki Won
KW
Lee
D000284:Administration, Oral; D000818:Animals; D016922:Cellular Senescence / Q000187:drug effects / Q000528:radiation effects; D000460:Chlorophyta / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D059748:Proteolysis; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects*; D013025:Glycine max / Q000737:chemistry*; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D010936:Plant Extracts; D019715:Tissue Inhibitor of Metalloproteinase-1; D003094:Collagen; D051057:Proto-Oncogene Proteins c-akt; D020781:Matrix Metalloproteinase 1
Haematococcus; photoaging; soybean; ultraviolet B