Synthesis of SiC/Ag/Cellulose Nanocomposite and Its Antibacterial Activity by Reactive Oxygen Species Generation.
We describe the synthesis of nanocomposites, based on nanofibers of silicon carbide, silver nanoparticles, and cellulose. Silver nanoparticle synthesis was achieved with chemical reduction using hydrazine by adding two different surfactants to obtain a nanocomposite with silver nanoparticles of different diameters. Determination of antibacterial activity was based on respiration tests. Enzymatic analysis indicates oxidative stress, and viability testing was conducted using an epifluorescence microscope. Strong bactericidal activity of nanocomposites was found against bacteriaEscherichia coliandBacillus cereus, which were used in the study as typical Gram-negative and Gram-positive bacteria, respectively. It is assumed that reactive oxygen species generation was responsible for the observed antibacterial effect of the investigated materials. Due to the properties of silicon carbide nanofiber, the obtained nanocomposite may have potential use in technology related to water and air purification. Cellulose addition prevented silver nanoparticle release and probably enhanced bacterial adsorption onto aggregates of the nanocomposite material.
Faculty of Geology, University of Warsaw, Żwirki i Wigury 93, 02-089 Warsaw, Poland. a.borkowski@uw.edu.pl.
Andrzej
A
Borkowski
Department of General and Environmental Microbiology, Poznań University of Life Sciences, ul. Szydłowska 50, 60-656 Poznań, Poland. tom.clapa@gmail.com.
Tomasz
T
Cłapa
Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego 2, 00-908 Warsaw, Poland. mateusz.szala@wat.edu.pl.
Mateusz
M
Szala
Faculty of Geology, University of Warsaw, Żwirki i Wigury 93, 02-089 Warsaw, Poland. agasin@uw.edu.pl.
Arkadiusz
A
Gąsiński
Department of General and Environmental Microbiology, Poznań University of Life Sciences, ul. Szydłowska 50, 60-656 Poznań, Poland. marek.selwet@gmail.com.
Marek
M
Selwet
Ag nanoparticles; antibacterial activity; cellulose; reactive oxygen species; silicon carbide nanocomposite