Evaluation ofToxoplasma gondiisoluble, whole and excretory/secretary antigens for diagnosis of toxoplasmosis by ELISA test.
The present study was performed to compare the soluble, whole and excretory/secretary antigens ofToxoplasma gondii(RH strain) in diagnosis of toxoplasmosis by ELISA method. Tachyzoites ofT. gondii, RH strain were injected in intra-peritoneal cavity of BALB/c mice, after 4 days tachyzoites were harvested by peritoneal washing of the mice. For soluble antigen, exudates were centrifuged and sediment sonicated and then centrifuged at 4 °C, 1 h, supernatant collected and density of protein determined by Bradford method. For whole antigen after collecting, washing and centrifuging of peritoneal fluid the tachyzoites sediment was counted. In excretory/secretary antigen 1.5 × 108tachyzoites were transferred in 1 ml tube of saline and incubated under mild agitation and after centrifuging, supernatant was collected and protein density determined by Bradford method. 176 human serum samples were evaluated forT. gondiiIgG antibody with prepared antigens, and finally serum samples were evaluated by commercial ELISA kit (Trinity, USA) which was considered as gold standard method. In this study sensitivity and specificity of prepared antigens compared with commercial kit in ELISA method. Sensitivity and specificity of soluble antigen was 91.4 and 74.5 %, in whole antigen these parameters were 77.1 and 77.3 % and in excretory/secretary antigen were 28.5 and 74.5 % respectively. Soluble antigen had high levels of sensitivity and specificity in ELISA method and the results were rather resemble to commercial kit (Trinity, USA).
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
S
S
Pishkari
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
S
S
Shojaee
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
H
H
Keshavarz
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
M
M
Salimi
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
M
M
Mohebali
ELISA; Excretory/secretary antigen; Soluble antigen; Toxoplasma gondii; Toxoplasmosis; Whole antigen