Evaluation of iron chelating and antioxidant potential of Epilobium hirsutum for the management of iron overload disease.
The present study deals with the investigation of iron chelating and antioxidant potential of Epilobium hirsutum in iron-overloaded rats. Iron overload was induced by 6 IP injections of Iron dextran (12.5mg/100g) administered uniformly over a period of 30 days. Different fractions of E. hirsutum were given orally and deferoxamine (DFO) subcutaneously for 30 days. The extent of iron chelation and various biochemical parameters were estimated on 15th and 30th day of treatment. In-vitro study was assessed by EDTA and DFO method; the results exhibited a dose-dependant iron chelation. The methanolic fraction of methanolic extract (MFME) and methanolic fraction of aqueous extract (MFAE) of E. hirsutum showed significant (p<0.01) iron chelating and antioxidant potential as compared to disease control (DC) rats. The animals treated with MFME and MFAE of E. hirsutum showed significant (p<0.01) vital organ protection as compared to DC rats. The animals treated for longer duration (30th day) reveals better iron chelation potential than shorter ones (15th day). Superior iron chelation was seen at higher dose (300mg/kg) as compared to lower dose (150mg/kg). Taken into an account, our result reveals the reversible iron chelating and antioxidant ability of E. hirsutum and gives some evidence for its possible mechanism via excretion of iron in urine and feces.
Department of Pharmacology, KYDSCT's College of Pharmacy, Sakegaon, Bhusawal 425201, Maharashtra, India; Department of Pharmacology, School of Pharmacy, RK University, Bhavnagar Highway, Kasturbadham, Rajkot 360020, Gujarat, India. Electronic address: wsheikh2@gmail.com.
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Sheikh
Department of Pharmacology, School of Pharmacy, RK University, Bhavnagar Highway, Kasturbadham, Rajkot 360020, Gujarat, India. Electronic address: trdesai@rku.ac.in.
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Desai
Department of Pharmacology, School of Pharmacy, RK University, Bhavnagar Highway, Kasturbadham, Rajkot 360020, Gujarat, India. Electronic address: pravin.tirgar@rku.ac.in.
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Tirgar
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003676:Deferoxamine / Q000494:pharmacology; D031666:Epilobium / Q000737:chemistry*; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology*; D019190:Iron Overload / Q000188:drug therapy*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D007502:Iron Chelating Agents; D007501:Iron; D003676:Deferoxamine
Antioxidant agent; Epilobium hirsutum; Iron chelating agents; Iron overload diseases; Onagraceae