Preparation and application of epitope magnetic molecularly imprinted polymers for enrichment of sulfonamide antibiotics in water.
Sulfonamides, which are widely used synthetic antibiotics, are hydrophilic and stable. They can easily migrate into the environment and aquatic animals, and increase the risk of cancer, drug resistance, and allergic symptoms if consumed by humans. Here, we developed an epitope magnetic imprinting approach to enrich multiple sulfonamide antibiotics from a water sample. Epitope magnetic molecularly imprinted polymers (EMMIPs) were prepared by free-radical polymerization using vinyl-functioned Fe3O4as a core, sulfanilamide (SA) as a dummy template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker. The performance of the EMMIPs was first evaluated by rebinding SA, and then an adsorption experiment was conducted to assess the extraction of multiple sulfonamide antibiotics containing the SA group. The binding experiments showed that the EMMIPs reached adsorption equilibrium in only 5 min with adsorption of SA at 2040 μg/g, compared with just 462 μg/g for the epitope magnetic non-imprinted polymers. EMMIPs were combined with HPLC for the detection of six sulfonamide antibiotics in surface water samples. The recoveries ranged from 79.3 to 92.4% and the relative standard deviations from 0.9 to 7.3%.
School of Food and Environment, Dalian University of Technology, Panjin, P. R. China.
Yufeng
Y
Hu
School of Food and Environment, Dalian University of Technology, Panjin, P. R. China.
Cheng
C
Wang
School of Food and Environment, Dalian University of Technology, Panjin, P. R. China.
Xiangdao
X
Li
School of Food and Environment, Dalian University of Technology, Panjin, P. R. China.
Lifen
L
Liu
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D000939:Epitopes; D052203:Ferrosoferric Oxide / Q000737:chemistry; D060328:Magnetic Phenomena; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*; D052616:Solid Phase Extraction / Q000379:methods; D013449:Sulfonamides / Q000302:isolation & purification*; D013499:Surface Properties; D014867:Water
D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D000939:Epitopes; D008689:Methacrylates; D011108:Polymers; D013449:Sulfonamides; D014867:Water; C008384:methacrylic acid; C004919:ethylene dimethacrylate; D052203:Ferrosoferric Oxide
Magnetic molecularly imprinted polymer; Sulfonamide antibiotics; Surface epitope imprinting