Microarray-based analysis of gene expression profiles in peripheral blood of patients with acute primary angle closure.
We investigated the expression of molecules in peripheral blood mononuclear cells (PBMCs) and plasma of patients with acute primary angle closure (APAC).



Peripheral blood was collected from patients with APAC (n = 10) and age-matched controls (n = 5). The gene transcription profile was analyzed in PBMCs using microarrays and validated by real-time reverse transcription polymerase chain reaction (RT-PCR). The levels of secreted proteins were evaluated in plasma by ELISA.



347 gene transcripts were up-regulated by 2-fold or more, and 696 transcripts down-regulated 2-fold or more in PBMCs from patients compared to controls. The most highly up-regulated gene was thrombospondin-1 (TSP-1, 8.66-fold increase), and the most down-regulated gene was prostaglandin-endoperoxide synthase 2 (PTGS2, 9.09-fold decrease). Real-time RT-PCR assay confirmed the increase of TSP-1 and the decrease of PTGS2 in PBMCs of patients. ELISA revealed that the levels of TSP-1 and active transforming growth factor (TGF)-β1 that is activated by TSP-1 were elevated in plasma of patients, while the level of prostaglandin E2 (PGE2) that is converted by PTGS2 was reduced. The plasma level of TSP-1 was positively correlated with that of active TGF-β1.



Our data suggest that the molecular network including TSP-1, TGF-β1, and PGE2 might be involved in the pathogenesis of APAC and PACG.
a Department of Ophthalmology , Seoul National University Hospital , Seoul , South Korea.
Jin Wook
JW
Jeoung
b Laboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center , Seoul National University Hospital Biomedical Research Institute , Seoul , South Korea.
Jung Hwa
JH
Ko
a Department of Ophthalmology , Seoul National University Hospital , Seoul , South Korea.
Yu Jeong
YJ
Kim
a Department of Ophthalmology , Seoul National University Hospital , Seoul , South Korea.
Yong Woo
YW
Kim
a Department of Ophthalmology , Seoul National University Hospital , Seoul , South Korea.
Ki Ho
KH
Park
a Department of Ophthalmology , Seoul National University Hospital , Seoul , South Korea.|b Laboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center , Seoul National University Hospital Biomedical Research Institute , Seoul , South Korea.
Joo Youn
JY
Oh
D000208:Acute Disease; D000368:Aged; D051546:Cyclooxygenase 2 / Q000235:genetics; D015232:Dinoprostone / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D015812:Glaucoma, Angle-Closure / Q000097:blood* / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019700:Thrombospondin 1 / Q000097:blood* / Q000235:genetics; D059467:Transcriptome / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000097:blood*
D012333:RNA, Messenger; D019700:Thrombospondin 1; D053773:Transforming Growth Factor beta1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone
Acute primary angle closure; peripheral blood mononuclear cells; prostaglandin E2; thrombospondin-1; transforming growth factor-β1