Defense response enhancement in strawberry via elicitors.
In this study, cell-suspension culture of strawberry (Fragaria × ananassa), cultivars Camarosa, and Sweet Charlie has been established. Embryogenic callus was induced by incubating the in vitro juvenile leaf explants on medium, containing 2-mg/l picloram at dark. Suspension culture was initiated from 4-week-old embryogenic calli in the liquid MS medium with 1-mg/l 2,4-D and 2-mg/l picloram. Suspension culture was maintained by sub-culturing each 3 weeks into a fresh medium. At week 9 after third sub-cultures, torpedo and cotyledonary embryo stages were observed. Embryos were then developed into shoots on medium 1 mg/l of each BA and IBA. Obtained shoots were successfully rooted on 1-mg/ml GA3, 0.5-mg/ml BA, and 1-mg/ml IBA. To enhance the resistance availability in strawberry plants, elicitation was applied by adding the JA and SA elicitors to the suspension culture with two doses (0.5 and 1 mM) individually and in combination, in addition to the fungal homogenate of Macrophomina phasiolena at concentration of 106spor/ml. The fawrky-1-Camarosa gene, which has defense-related function, was detected in the different elicited strawberry tissues and isolated via RT-PCR. The isolated gene was submitted to GenBank with accession number (KX096885).
Gene Transfer Lab, Plant Genetic Transformation Department, Agricultural Genetic Engineering Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. gigihosny@gmail.com.
Gihan M H
GM
Hussein
Botany and Microbiolgy Department, Faculty of Science, Cairo University, Giza, Egypt.
Tahany M A
TM
Abdel-Rahman
Botany and Microbiolgy Department, Faculty of Science, Cairo University, Giza, Egypt.|Biology Department, College of Science, AL-Mustansiriya University, Baghdad, Iraq.
A H
AH
Alwan
Elicitors; Jasmonic acid; Salicyilic acid fawrky gene; Strawberry cell-suspension culture