Ferrocene-graphene sheets for high-efficiency quenching of electrochemiluminescence from Au nanoparticles functionalized cadmium sulfide flower-like three dimensional assemblies and sensitive detection of prostate specific antigen.
A signal-switchable electrochemiluminescence (ECL) aptasensor was presented for sensitive prostate specific antigen (PSA) assay using ferrocene-graphene sheets (Fc-GNs) for high-efficiency quenching of ECL from Au nanoparticles functionalized cadmium sulfide flower-like three dimensional (3D) assemblies (Au-CdS flower-like 3D assemblies). Au-CdS flower-like 3D assemblies were synthesized and employed as luminophore, exhibiting strong and stable ECL intensity, and followed by assembling captured DNA (cDNA) and hybridizing it with half of base sequence of PSA aptamer on the Au-CdS flower-like 3D assemblies modified electrode. The remaining part of the non-complementary base of the aptamer could preferentially adsorb GN with the signal switched "off" state. While in the presence of the PSA, the binding of PSA with aptamer caused desorption of aptamer from the surface of Fc-GNs and was then released from electrode surface, thus allowing the ECL signal enhancement. With the transformation of luminescence signal from "off" to "on", the aptasensor displays high sensitivity for PSA detection with a linear range from 1pgmL-1to 25ngmL-1and a detection limit of 0.38pgmL-1S/N=3). Moreover, this developed method could be successfully applied to the determination of PSA in human serum samples with recoveries of 85.8-104.0%, suggesting great potential applications in biochemical analysis.
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, PR China.
Jiu-Jun
JJ
Yang
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, PR China. Electronic address: jtcao11@163.com.
Jun-Tao
JT
Cao
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, PR China.
Hui
H
Wang
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, PR China. Electronic address: liuym9518@sina.com.
Yan-Ming
YM
Liu
Xinyang Central Hospital, Xinyang 464000, PR China.
Shu-Wei
SW
Ren
D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry*; D000075163:Metallocenes / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000378:metabolism; D013440:Sulfides / Q000737:chemistry*; D013890:Thiourea / Q000737:chemistry
D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D005296:Ferrous Compounds; D000075163:Metallocenes; D013440:Sulfides; C034939:cadmium sulfide; D006046:Gold; D006108:Graphite; D017430:Prostate-Specific Antigen; D013890:Thiourea; C004998:ferrocene
Au-CdS flower-like 3D assemblies; Electrochemiluminescence aptasensor; Ferrocene-graphene sheets; Prostate specific antigen; Signal-switchable strategy