Evaluation of the bioNexia Legionella Test, Including Impact of Incubation Time Extension, for Detection of Legionella pneumophila Serogroup 1 Antigen in Urine.
In this study, we compared the bioNexia test (bioMérieux, Marcy-l'Étoile, France), a new immunochromatographic assay for the detection ofLegionella pneumophilaserogroup 1 in urine, with the BinaxNOW urinary antigen test (Alere, Waltham, Massachusetts, USA). After 15 min of incubation (in accordance with the manufacturers' instructions), the sensitivities and specificities were, respectively, 76.5% and 97.2% for the bioNexia test and 87.1% and 100% for the BinaxNOW test. After a prolonged incubation time of 60 min, the sensitivities and specificities increased to, respectively, 89.4% and 97.2% for the bioNexia test and 91.8% and 100% for the BinaxNOW test. When the tests were read after 15 min, the concentration of discrepant urine samples increased the sensitivities to 94.1% for both tests. In conclusion, we found that although the bioNexia test showed lower sensitivity for the detection ofL. pneumophilaantigen in nonconcentrated urine compared to the BinaxNOW test, a prolonged incubation time as well as the use of concentrated samples showed comparable sensitivities for both tests.
Regional Public Health Laboratory Kennemerland, Boerhaavelaan, Haarlem, the Netherlands p.badoux@streeklabhaarlem.nl.
Paul
P
Badoux
Regional Public Health Laboratory Kennemerland, Boerhaavelaan, Haarlem, the Netherlands.
Sjoerd M
SM
Euser
Regional Public Health Laboratory Kennemerland, Boerhaavelaan, Haarlem, the Netherlands.
Jacob P
JP
Bruin
Regional Public Health Laboratory Kennemerland, Boerhaavelaan, Haarlem, the Netherlands.
Patrick P G
PPG
Mulder
Regional Public Health Laboratory Kennemerland, Boerhaavelaan, Haarlem, the Netherlands.
Ed P F
EPF
Yzerman
D000942:Antigens, Bacterial / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D016952:Legionella pneumophila / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D065288:Serogroup; D013997:Time Factors; D014556:Urine / Q000737:chemistry*
D000942:Antigens, Bacterial
Legionella; Legionnaires' disease; urinary antigen test