Molecular characterization and phylogenetic analysis ofPlasmodium vivax,Plasmodium falciparum,Plasmodium ovale,Plasmodium malariaeandPlasmodium cynomolgi.
18S ribosomal RNA gene sequences of different species ofPlasmodiumwere aligned and analyzed to determine the molecular diversity among different species ofPlasmodium. AT content ofP.cynomolgi,P.ovale,P.falciparum,P.vivaxandP.malariaeranged from 62.30 to 63.15, 63.90 to 65.29, 66.67 to 68.40, 61.66 to 63.25 and 64.09 to 76.36 in case respectively. GC content ofP.cynomolgi,P.ovale,P.falciparum,P.vivaxandP.malariaeranged from 36.85 to 37.70, 34.71 to 36.43, 31.60 to 33.27, 23.64 to 35.91 and 36.75 to 38.34 respectively. Molecular weight (Da) ofP.cynomolgi,P.ovale,P.falciparum,P.vivaxandP.malariaeranged from 1,160,127.00 to 1,400,332.00, 437,273.00 to 481,438.00, 668,021.00 to 685,243.00, 540,378.00 to 1,176,962.00 and 132,788.00 to 305,211.00 respectively. Relative melting temperature (Tm) ofP.cynomolgiandP.ovalevaried from 0.36799 to 0.36837 and 0.36712 to 0.36814 respectively. InP.falciparumandP.vivaxrelative Tm ranged from 0.36545 to 0.36626 and 0.36802 to 0.36866 respectively. Relative Tm ofP.malariaevaried from 0.36174 to 0.36790.
Parasitology and Microbiology Research Laboratory, Department of Zoology, Burdwan University, Burdwan, West Bengal India.
Soumendranath
S
Chatterjee
Parasitology and Microbiology Research Laboratory, Department of Zoology, Burdwan University, Burdwan, West Bengal India.
Priyanka
P
Mukhopadhyay
Department of Nutrition, A.K.P.C Mahavidyalaya, Bengai, Hooghly, West Bengal India.
Raktima
R
Bandyopadhyay
Parasitology and Microbiology Research Laboratory, Department of Zoology, Burdwan University, Burdwan, West Bengal India.
Paltu
P
Dhal
Parasitology and Microbiology Research Laboratory, Department of Zoology, Burdwan University, Burdwan, West Bengal India.
Debraj
D
Biswal
Parasitology Laboratory, Department of Zoology, University of Kalyani, Nadia, Kalyani, West Bengal India.
Prabir Kumar
PK
Bandyopadhyay
AT content; Different species of Plasmodium; GC content; Molecular phylogeny; Relative melting temperature (Tm)