Redox and Reactive Oxygen Species Network in Acclimation for Salinity Tolerance in Sugar Beet.
Fine-tuned and coordinated regulation of transport, metabolism and redox homeostasis allows plants to acclimate to osmotic and ionic stress caused by high salinity. Sugar beet is a highly salt tolerant crop plant and is therefore an interesting model to study sodium chloride (NaCl) acclimation in crops. Sugar beet plants were subjected to a final level of 300 mM NaCl for up to 14 d in hydroponics. Plants acclimated to NaCl stress by maintaining its growth rate and adjusting its cellular redox and reactive oxygen species (ROS) network. In order to understand the unusual suppression of ROS accumulation under severe salinity, the regulation of elements of the redox and ROS network was investigated at the transcript level. First, the gene families of superoxide dismutase (SOD), peroxiredoxins (Prx), alternative oxidase (AOX), plastid terminal oxidase (PTOX) and NADPH oxidase (RBOH) were identified in the sugar beet genome. Salinity induced the accumulation of Cu-Zn-SOD, Mn-SOD, Fe-SOD3, all AOX isoforms, 2-Cys-PrxB, PrxQ, and PrxIIF. In contrast, Fe-SOD1, 1-Cys-Prx, PrxIIB and PrxIIE levels decreased in response to salinity. Most importantly, RBOH transcripts of all isoforms decreased. This pattern offers a straightforward explanation for the low ROS levels under salinity. Promoters of stress responsive antioxidant genes were analyzed in silico for the enrichment of cis-elements, in order to gain insights into gene regulation. The results indicate that special cis-elements in the promoters of the antioxidant genes in sugar beet participate in adjusting the redox and ROS network and are fundamental to high salinity tolerance of sugar beet.
Department of Biochemistry and Physiology of Plants, Faculty of Biology, University of Bielefeld, D-33501 Bielefeld, Germany.
M Sazzad
MS
Hossain
Department of Biochemistry and Physiology of Plants, Faculty of Biology, University of Bielefeld, D-33501 Bielefeld, Germany.|Biochemistry Department, Faculty of Agriculture, Zagazig University, 44519 Zagazig, Egypt.
Abdelaleim Ismail
AI
ElSayed
Department of Biochemistry and Physiology of Plants, Faculty of Biology, University of Bielefeld, D-33501 Bielefeld, Germany.
Marten
M
Moore
Department of Biochemistry and Physiology of Plants, Faculty of Biology, University of Bielefeld, D-33501 Bielefeld, Germany.
Karl-Josef
KJ
Dietz
D000064:Acclimatization; D027461:Beta vulgaris / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D010084:Oxidation-Reduction; D010802:Phylogeny; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055049:Salt Tolerance; D017422:Sequence Analysis, DNA
D017382:Reactive Oxygen Species
Alternative oxidase; NADPH oxidase; RBOH; antioxidant defense; peroxiredoxin; salinity stress; sugar beet; superoxide dismutase