Effects of butyric acid and arsenic on isolated pancreatic islets and liver mitochondria of male mouse.
OBJECTIVE
The aim of the present study was to evaluate the different doses of Butyric acid (BA) and Arsenic (As) in liver mitochondria oxidative stress and pancreatic islet insulin secretion of male mouse.


BACKGROUND
BA is found in many foods and As as a toxic metal is present in drinking water. They can induce oxidative stress in tissues.


METHODS
In this experimental study, Liver mitochondria were isolated by administration of the different centrifugation method and pancreatic islets were isolated by collagenase method. Mitochondria were incubated by BA (35, 75, 150, 300 μM) and As (20, 50, 100, 200 μM) as the islets were incubated by BA (250, 500, 1000, 1500 μM) and As (50, 100, 200 μM) for 1 hour. At the end of the experiment, mitochondrial viability and membrane potential, ROS, MDA, GSH and islets insulin secretion were measured by their specific methods.


RESULTS
BA and As administration increased mitochondrial levels of ROS, MDA and decreased GSH and pancreatic islet insulin secretion in a dose dependent manner (p<0.05). The doses of BA 75μM and As 100μM have been revealed the most mitochondria toxic concentrations. Also, the doses of 1000μM for BA and 100μM for As were considered as reducing concentrations for islets insulin secretion. Additionally, co administration of them intensified more these effects.


CONCLUSIONS
Alone or in combination administration of BA and As induced oxidative stress in liver mitochondria and decreased insulin secretion of pancreatic islets.
Health Research Institute, Diabetes Research Center, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Akram
A
Ahangarpour
Department of Physiology, Student Research Committee of Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.
Ali Akbar
AA
Oroojan
Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Mohsen
M
Rezae
Department of Pharmacology and Toxicology, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mohammad Javad
MJ
Khodayar
Department of Pharmacology, School of Pharmacy, Student Research Committee of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Soheila
S
Alboghobeish
Department of Toxicology , School of Pharmacy , Student Research Committee of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Marzieh
M
Zeinvand
Arsenic; Butyric acid; Islet; Liver mitochondria; Mouse