Magnetic nanoparticles for efficient cell transduction with Semliki Forest virus.
Semliki Forest virus (SFV) is a potential cancer gene therapy vector capable of providing high and transient expression of heterologous proteins in mammalian cells. However, SFV has shown suboptimal transduction levels in several cancer cell types as well as wide biodistribution of SFV has been observed after in vivo applications. Magnetic nanoparticles (MNPs) have been shown to increase cell transduction with several viral vectors in vitro under an external magnetic field and enhance magnetically guided viral vector delivery. Here, we examined a panel of MNPs for enhanced cancer cell transduction with SFV vector. Magneto-transduction using positively charged MNPs increased Semliki Forest virus transduction in TS/A mouse mammary carcinoma cells in vitro in the presence of fetal bovine serum. Positively charged MNPs efficiently captured SFV particles independently of capturing medium, and MNPs-SFV complexes were successfully separated from suspension by magnetic precipitation. These results reveal the potential application of MNPs for enhanced gene delivery by SFV vector as well as proposes magnetic precipitation for efficient concentration of SFV particles from different media.
Latvian Biomedical Research and Study Centre, Ratsupites Street 1, LV-1067 Riga, Latvia.
Baiba
B
Kurena
Latvian Biomedical Research and Study Centre, Ratsupites Street 1, LV-1067 Riga, Latvia.
Aleksandra
A
Vežāne
Latvian Biomedical Research and Study Centre, Ratsupites Street 1, LV-1067 Riga, Latvia.
Dace
D
Skrastiņa
Latvian Biomedical Research and Study Centre, Ratsupites Street 1, LV-1067 Riga, Latvia.
Olga
O
Trofimova
Latvian Biomedical Research and Study Centre, Ratsupites Street 1, LV-1067 Riga, Latvia. Electronic address: anna@biomed.lu.lv.
Anna
A
Zajakina
D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D058185:Magnetite Nanoparticles; D008325:Mammary Neoplasms, Experimental / Q000821:virology; D051379:Mice; D012672:Semliki forest virus / Q000235:genetics*; D014161:Transduction, Genetic / Q000379:methods*
D058185:Magnetite Nanoparticles
Alphavirus; Cancer gene therapy; Magnetic nanoparticles; Transduction; Virus