[Effect of low dose erythromycin on the proliferation of granulation tissue after tracheal injury].
Objective:To investigate the effect of low dose erythromycin on the proliferation of granulation tissue after tracheal injury.Methods:Forty-two rabbits were randomly divided into 7 groups (n=6 each), group A (saline control group), group B (penicillin group), group C (low dose erythromycin group), group D (low dose erythromycin and penicillin group), group E (budesonide group), group F (low dose erythromycin and budesonide group), group G (low dose erythromycin, penicillin and budesonide group). All rabbits received tracheotomy, and the tracheal mucosa was scraped with a nylon brush 20 times for tracheal stenosis model. Rabbits were treated with corresponding drugs from a week before operation to 9 days after operation. The serum concentrations of transforming growth factor - beta 1 (TGF-β(1)), vascular endothelial growth factor (VEGF), interleukin (IL) -6, IL-8 were determined and the tracheal specimens were harvested for measuring degree of stenosis on the 10th day after operation.Results:Serum concentrations of TGF-β(1) in group A, B, C, D, E, F and G were (17.6±1.3), (18.2±3.1), (13.0±1.1), (14.0±1.0), (21.0±6.1), (13.6± 3.5), (8.2±1.3) ng/L; VEGF were (88.1±4.1), (85.8±4.3), (58.1±6.3), (56.5±2.4), (87.8±2.8), (57.0±3.7), (34.3±6.7) ng/L; IL-6 were (67.8±4.0), (66.1±3.5), (54.1±4.8), (52.1±3.2), (64.6±4.9), (49.4±4.2), (35.9±3.7) ng/L; IL-8 were (112.8±5.2), (116.6±4.1), (88.0±6.2), (85.5±3.5), (114.4±4.6), (82.6±3.8), (55.9±6.0) ng/L, respectively. The serum concentrations of TGF-β(1), VEGF, IL-6 and IL-8 in group C, D, F and G were significantly lower than those in group A, B and E (allP<0.05). Compared with the other groups, the serum concentrations in group G were the lowest (allP<0.05). All 42 rabbits had tracheal stenosis with different degrees of proliferation of granulation tissue. The degree of tracheal stenosis in Group A, B, C, D, E, F and G were (53.3±4.4)%, (48.2±5.0)%, (24.3±4.4)%, (29.5±3.2)%, (47.8±6.5)%, (27.9±3.1)%, (15.6±2.0)%, respectively. The degree of tracheal stenosis in group C, D, F and G was significantly lower than that in group A, B and E, which had statistical differences (allP<0.05). Compared with the other groups, the degree of tracheal stenosis in group G was the lowest (allP<0.05).Conclusions:Low dose of erythromycin can effectively inhibit the proliferation of granulation tissue after tracheal injury in rabbits. And it has better effectiveness when combined with other antibiotics and hormone.
Department of Respiratory, Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, China.
L H
LH
Li
M P
MP
Xu
L M
LM
Gan
Y
Y
Li
Y L
YL
Liang
W T
WT
Li
E Y
EY
Qin
J H
JH
Gan
G N
GN
Liu
D000818:Animals; D000900:Anti-Bacterial Agents; D019819:Budesonide; D049109:Cell Proliferation; D004917:Erythromycin / Q000031:analogs & derivatives; D006097:Granulation Tissue; D015850:Interleukin-6; D011817:Rabbits; D014132:Trachea; D014135:Tracheal Stenosis; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A
D000900:Anti-Bacterial Agents; D015850:Interleukin-6; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; C474718:erythromycin G; D019819:Budesonide; D004917:Erythromycin
Erythromycin; Granulation tissue; Inflammation; Tracheal stenosis; Wounds and injuries