De novo sequencing and comparative analysis of leaf transcriptomes of diverse condensed tannin-containing lines of underutilized Psophocarpus tetragonolobus (L.) DC.
Condensed tannin (CT) or proanthocyanidin (PA) is a unique group of phenolic metabolite with high molecular weight with specific structure. It is reported that, the presence of high-CT in the legumes adversely affect the nutrients in the plant and impairs the digestibility upon consumption by animals. Winged bean (Psophocarpus tetragonolobus (L.) DC.) is one of the promising underutilized legume with high protein and oil-content. One of the reasons for its underutilization is due to the presence of CT. Transcriptome sequencing of leaves of two diverse CT-containing lines of P. tetragonolobus was carried out on Illumina Nextseq 500 sequencer to identify the underlying genes and contigs responsible for CT-biosynthesis. RNA-Seq data generated 102586 and 88433 contigs for high (HCTW) and low CT (LCTW) lines of P. tetragonolobus, respectively. Based on the similarity searches against gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) database revealed 5210 contigs involved in 229 different pathways. A total of 1235 contigs were detected to differentially express between HCTW and LCTW lines. This study along with its findings will be helpful in providing information for functional and comparative genomic analysis of condensed tannin biosynthesis in this plant in specific and legumes in general.
Plant Molecular Biology and Genetic Engineering Division, CSIR-National Botanical Research Institute, Lucknow-226 001 Uttar Pradesh, India.|Department of Biotechnology, Kumaun University, Nainital, Uttarakhand, 263001 India.
Vinayak
V
Singh
Plant Molecular Biology and Genetic Engineering Division, CSIR-National Botanical Research Institute, Lucknow-226 001 Uttar Pradesh, India.
Ridhi
R
Goel
Department of Biotechnology, Kumaun University, Nainital, Uttarakhand, 263001 India.
Veena
V
Pande
Plant Molecular Biology and Genetic Engineering Division, CSIR-National Botanical Research Institute, Lucknow-226 001 Uttar Pradesh, India.
Mehar Hasan
MH
Asif
Plant Molecular Biology and Genetic Engineering Division, CSIR-National Botanical Research Institute, Lucknow-226 001 Uttar Pradesh, India.
Chandra Sekhar
CS
Mohanty
D053898:Biosynthetic Pathways / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D020451:Contig Mapping; D007887:Fabaceae / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics; D058977:Molecular Sequence Annotation; D018515:Plant Leaves / Q000235:genetics*; D044945:Proanthocyanidins / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D014157:Transcription Factors / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*
D044945:Proanthocyanidins; D012333:RNA, Messenger; D014157:Transcription Factors