Set up of cutoff thresholds for kinship determination using SNP loci.
The usefulness of single nucleotide polymorphism (SNP) loci for kinship testing has been demonstrated in many case works, and suggested as a promising marker for relationship identification. For interpreting results based on the calculation of the likelihood ratio (LR) in kinship testing, it is important to prepare cutoffs for respective relatives which are dependent on genetic relatedness. For this, analysis using true pedigree data is significant and reliable as it reflects the actual frequencies of markers in the population. In this study, the kinship index was explored through 1209 parent-child pairs, 1373 full sibling pairs, and 247 uncle-nephew pairs using 136 SNP loci. The cutoffs for LR were set up using different numbers of SNP loci with accuracy, sensitivity, and specificity. It is expected that this study can support the application of SNP loci-based kinship testing for various relationships.
Institute of Forensic Science, Seoul National University, College of Medicine, Seoul, South Korea.
Sohee
S
Cho
DNA Link, Seoul, South Korea.
Eun Soon
ES
Shin
DNA Link, Seoul, South Korea.
Hyung Jin
HJ
Yu
Department of Forensic Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
Ji Hyun
JH
Lee
Department of Forensic Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
Hee Jin
HJ
Seo
Department of Forensic Medicine, Seoul National University, College of Medicine, Seoul, South Korea.
Moon Young
MY
Kim
Institute of Forensic Science, Seoul National University, College of Medicine, Seoul, South Korea; Department of Forensic Medicine, Seoul National University, College of Medicine, Seoul, South Korea. Electronic address: sdlee@snu.ac.kr.
Soong Deok
SD
Lee
D044466:Asian People / Q000235:genetics; D053803:Forensic Genetics; D006801:Humans; D016013:Likelihood Functions; D015810:Linkage Disequilibrium; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D056910:Republic of Korea
Full-sibling; Kinship; Likelihood ratio; Single nucleotide polymorphism; Uncle-nephew