Canine tick-borne diseases in pet dogs from Romania.
BACKGROUND
Tick-borne diseases are of substantial concern worldwide for animals as well as humans. Dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. Tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. In addition, co-infections with two or more pathogens enhance this problem further. Molecular methods are useful to disentangle co-infections and to accurately describe prevalence and geographical distribution of tick-borne diseases. At this point, this information is lacking in many areas worldwide. Romania is one such area, where prevalence and distribution of several important pathogens need to be further investigated. To address this, we screened blood samples from 96 sick dogs with molecular methods for eight different pathogens including Babesia spp., Theileria spp., Hepatozoon spp., Anaplasma spp., Ehrlichia spp., "Candidatus Neoehrlichia mikurensis", Mycoplasma spp., and Borrelia spp.


RESULTS
As many as 45% (43/96) of the dogs in the study were infected with protozoan parasites. Babesia canis was the most frequent of these (28 infected dogs), whereas Hepatozoon canis was detected in 15% (14/96) and Babesia gibsoni was found in a single sample. Bacterial infection with Mycoplasma spp. occurred in 18% (17/96) of the sampled dogs. Obtained bacterial sequences revealed the occurrence of two species: Mycoplasma canis and "Candidatus Mycoplasma haematoparvum". In several cases co-infection with protozoan parasites and Mycoplasma sp. were detected. All dogs were negative for Anaplasma spp., Ehrlichia spp., "Ca. Neoehrlichia mikurensis", and for Borrelia spp.


CONCLUSIONS
The results from the present study reinforce the notion that Babesia canis is an important pathogen in the Romanian dog population. However, more surprisingly, another protozoan species, H. canis, seems to be infecting dogs to a larger extent than previously recognized in Romania. Well-known tick-borne bacterial disease agents such as Anaplasma spp. and Borrelia spp. were not detected. In contrast, less well-studied bacteria such as hemotropic Mycoplasma spp. were detected frequently. Moreover, co-infection might aggravate disease and complicate diagnosis and should be further studied in dogs.
Center for Ecology and Evolution in Microbial Model Systems (EEMiS), Linnaeus University, SE-391 82, Kalmar, Sweden.
Martin O
MO
Andersson
Center for Ecology and Evolution in Microbial Model Systems (EEMiS), Linnaeus University, SE-391 82, Kalmar, Sweden.
Conny
C
Tolf
Institute for Diagnosis and Animal Health, Bucharest, Romania.
Paula
P
Tamba
PAUMI-VET Private Veterinary Clinics, Snagov, Ilfov County, Romania.
Mircea
M
Stefanache
Center for Ecology and Evolution in Microbial Model Systems (EEMiS), Linnaeus University, SE-391 82, Kalmar, Sweden.
Jonas
J
Waldenström
Bundeswehr Institute of Microbiology, German Center of Infection Research (DZIF) Partner, Neuherbergstrasse 11, D-80937, Munich, Germany. gerharddobler@msn.com.
Gerhard
G
Dobler
Institute for Diagnosis and Animal Health, Bucharest, Romania.|Bundeswehr Institute of Microbiology, German Center of Infection Research (DZIF) Partner, Neuherbergstrasse 11, D-80937, Munich, Germany.
Lidia
L
Chițimia-Dobler
D000818:Animals; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000469:parasitology; D004285:Dogs; D057805:Pets; D015995:Prevalence; D011529:Protozoan Infections, Animal / Q000453:epidemiology* / Q000469:parasitology; D012383:Romania / Q000453:epidemiology; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary*
Canis familiaris; Dogs; Romania; Tick-borne diseases; Vector-borne diseases