Antibacterial and antibiotic resistance modulatory activities of leaves and bark extracts of Recinodindron heudelotii (Euphorbiaceae) against multidrug-resistant Gram-negative bacteria.
BACKGROUND
Recinodindron heudelotii (Euphorbiaceae) is a plant used in Africa, particularly in Cameroon to treat various ailments including bacterial infections. In this study, we evaluated the extracts of the leaves (RHL) and bark (RHB) of R. heudelotii for their antibacterial and antibiotic resistance modulating activities against 29 Gram-negative bacteria, including multidrug-resistant (MDR) phenotypes.


METHODS
The broth micro-dilution assay was used to evaluate the antibacterial activity, and the antibiotic resistance modulating effects of the plant extracts.


RESULTS
RHL displayed the most important spectrum of activity with minimal inhibitory concentrations (MICs) values ranging from 256 to 1024 μg/mL against 75.86% of the 29 tested bacteria strains while RHB was not active. RHL also showed killing effects with minimal bactericidal concentrations (MBCs) ranging from 256 to 1024 μg/mL. The activities of tetracycline and kanamycin associated with RHL were improved on 88.89% and 77.78% of the tested MDR bacteria, at MIC/2 at MIC/4 respectively, with 2 to 16-folds decreasing of MIC. This suggests the antibiotic resistance modulating effects of these antibiotics.


CONCLUSIONS
The present study provides data indicating a possible use of the leaves extract of Recinodindron heudelotii alone or in association with common antibiotics in the fight against bacterial infections including those involving MDR bacteria.
Department of Biochemistry, Faculty of science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Aimé Gabriel
AG
Fankam
Department of Biochemistry, Faculty of science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Jules-Roger
JR
Kuiate
Department of Biochemistry, Faculty of science, University of Dschang, P.O. Box 67, Dschang, Cameroon. kuetevictor@yahoo.fr.
Victor
V
Kuete
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D019665:Euphorbiaceae / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D007612:Kanamycin; D013752:Tetracycline
Recinodindron heudelotii, Gram-negative bacteria, MDR bacteria; antibacterial activity; antibiotic modulators