The Development and Validation of Novel, Simple High-Performance Liquid Chromatographic Method with Refractive Index Detector for Quantification of Memantine Hydrochloride in Dissolution Samples.
The present study was aimed to develop an analytical method for quantification of memantine (MEM) hydrochloride in dissolution samples using high-performance liquid chromatography with refractive index (RI) detector. The chromatographic separation was achieved on C18 (250 × 4.5 mm, 5 μm) column using isocratic mobile phase comprises of buffer (pH 5.2):methanol (40:60 v/v) pumped at a flow rate of 1.0 mL/min. The column effluents were monitored using RI detector. The retention time of MEM was found to be ~6.5 ± 0.3 min. The developed chromatographic method was validated and found to be linear over the concentration range of 5.0-45.0 μg/mL for MEM. Mean recovery of MEM was found to be 99.2 ± 0.5% (w/w). The method was found to be simple, fast, precise and accurate, which can be utilized for the quantification of MEM in dissolution samples.
Wockhardt Research Centre (Global Technical Services), Aurangabad 431210, Maharashtra, India.|Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, Maharashtra, India.
Tukaram B
TB
Sawant
Dr. Babasaheb Ambedkar Marathwada University, Aurangabad431004, Maharashtra, India.
Vikas S
VS
Wakchaure
Wockhardt Research Centre (Global Technical Services), Aurangabad 431210, Maharashtra, India.|Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, Maharashtra, India.
Udyakumar K
UK
Rakibe
Wockhardt Research Centre (Global Technical Services), Aurangabad431210, Maharashtra, India.
Prashant B
PB
Musmade
Zulal Bhilajirao Patil College, Z.B.Patil road, Deopur, Dhule424002, Maharashtra, India.
Bhata R
BR
Chaudhari
Dr. Babasaheb Ambedkar Marathwada University, Aurangabad431004, Maharashtra, India.
Dhananjay V
DV
Mane
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D008559:Memantine / Q000032:analysis* / Q000737:chemistry; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility
D008559:Memantine