Long non-coding RNA 00312 regulated by HOXA5 inhibits tumour proliferation and promotes apoptosis in Non-small cell lung cancer.
Non-small cell lung cancer (NSCLC) is the most prevalent type of lung cancer. The abnormal expression of many long non-coding RNAs (lncRNAs) has been reported involved in the progression of various tumours, which can be used as diagnostic indicators or antitumour targets. Here, we found that the long non-coding RNA 00312 was down-regulated in paired NSCLC tissues and correlated with poor clinical outcome; decreased linc00312 expression in NSCLC was associated with larger and later stage tumours. Functional experiments showed that linc00312 could inhibit cell proliferation and promote apoptosis in vitro and in vivo. Furthermore, we found that HOXA5 could bind in the promoter of linc00312 and up-regulated the expression of it. Moreover, linc00312 was down-regulated in the plasma of NSCLC patients compared with that of healthy volunteers or other pulmonary diseases patients. Taken together, our findings indicated that linc00312 could be a novel diagnosis biomarker and a promising therapeutic target for NSCLC.
Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.|Nanjing University Institute of Respiratory Medicine, Nanjing, China.
Qingqing
Q
Zhu
Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.|Nanjing University Institute of Respiratory Medicine, Nanjing, China.
Tangfeng
T
Lv
Department of Respiratory Medicine, Zhongda Hospital, Southeast University School of Medicine, Nanjing, China.
Ying
Y
Wu
Department of Respiratory Medicine, Huzhou Central Hospital, Huzhou, China.
Xuefei
X
Shi
Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.|Nanjing University Institute of Respiratory Medicine, Nanjing, China.
Hongbing
H
Liu
Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.|Nanjing University Institute of Respiratory Medicine, Nanjing, China.
Yong
Y
Song
D000107:Acetylation; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D030541:Databases, Genetic; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008745:Methylation; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
C489213:HOXA5 protein, human; D018398:Homeodomain Proteins; C470228:LINC00312 long non-coding RNA, human; D062085:RNA, Long Noncoding

NCSLC
; HOXA5; apoptosis; linc00312; lncRNA; proliferation