Parthenolide attenuates cerebral ischemia/reperfusion injury via Akt/GSK-3β pathway in PC12 cells.
Parthenolide (PN), a sesquiterpene lactone isolated from the herbal medicine feverfew (Tanacetum parthenium), was reported to possess neuroprotective activity. However, the neuroprotective effect of PN against cerebral ischemia/reperfusion (I/R) injury remains unclear. Therefore, the aim of the present study was to explore the neuroprotective effects of PN against oxygen-glucose deprivation (OGD)-induced apoptosis in PC12 cells and the underlying mechanisms. Our results demonstrated that PN ameliorated OGD/R-evoked neuronal injury and oxidative stress in PC12 cells. In addition, PN notably decreased HIF-1α expression, as well as inhibited apoptosis in PC12 cells after OGD/R. Furthermore, PN pretreatment significantly enhanced the phosphorylation of Akt and GSK-3β in PC12 cells exposed to OGD/R. In conclusion, the present study demonstrated that PN exhibits a neuroprotective effect against OGD/R through activation of the Akt/GSK-3β signaling pathway. Our findings suggest that PN has the potential to serve as a novel therapeutic agent for cerebral I/R injury.
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Jun-Feng
JF
Zhang
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Li
L
Zhang
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Li-Li
LL
Shi
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Zhao-Hua
ZH
Zhao
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Hao
H
Xu
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Fei
F
Liang
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Hong-Bo
HB
Li
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China.
Yan
Y
Zhao
Department of Human Anatomy, Xi'an Medical University, Xi'an 710021, People's Republic of China. Electronic address: xx_anato@163.com.
Xi
X
Xu
Department of Anesthesiology, The No.1 Hospital of Xi'an, Xi'an 710002, People's Republic of China. Electronic address: yangkeke_yk@163.com.
Ke
K
Yang
College of Life Sciences, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Ying-Fang
YF
Tian
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D005947:Glucose / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D000066829:Neuroprotection / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D016716:PC12 Cells; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
D018696:Neuroprotective Agents; D012717:Sesquiterpenes; C002669:parthenolide; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose; D010100:Oxygen
Apoptosis; Cerebral ischemia/reperfusion (I/R); Oxygen-glucose deprivation (OGD); Parthenolide (PN)