Stepwise bioprocess for exopolysaccharide production using potato starch as carbon source.
Xanthan gum is a biopolymer produced by Xanthomonas sp. XC6. In this study, xanthan gum is produced from potato starch using a stepwise bioprocess design. Potato starch is hydrolyzed using Bacillus sp. having amylase activity and 30.2 g/L reducing sugar was released, while Xanthomonas sp. XC6 can release only 14.5 g/L. Bacillus sp. hydrolyzed potato starch extract was further used as a carbon source for xanthan gum biosynthesis using Xanthomonas sp. XC6. Yeast extract acts as the best nitrogen source, and 10.0 g/L xanthan gum was recovered. Downstreaming process after stepwise bioprocess resulted in 17.4 g/L xanthan gum production, which is 2.8 times higher as compared to single step process.
Department of Biotechnology, Indian Institute of Education, Ghanahatti, Shimla, 171011, India. shashibiotechhpu@gmail.com.|Department of Biotechnology, Himachal Pradesh University, Shimla, 171005, India. shashibiotechhpu@gmail.com.
Shashi Kant
SK
Bhatia
Department of Biotechnology, Indian Institute of Education, Ghanahatti, Shimla, 171011, India.
Narinder
N
Kumar
Department of Biotechnology, Himachal Pradesh University, Shimla, 171005, India.
Ravi Kant
RK
Bhatia
Biopolymer; Biosynthesis; Downstreaming process; Xanthan gum