Determination of hymexazol in 26 foods of plant origin by modified QuEChERS method and liquid chromatography tandem-mass spectrometry.
A rapid and sensitive method based on modified QuEChERS for hymexazol determination in 26 plant-derived foods using liquid chromatography tandem-mass spectrometry (LC-MS/MS) was developed. Variables affecting the separation (LC column, mobile phase additives) and clean-up effects of various dispersive phases, such as PSA, C18, GCB, MWCNTs, PEP-2, Al2O3, Florisil, and PVPP were evaluated. The method was validated using 26 matrices at spiked levels of 0.01 or 0.02, 0.05, 0.1, and 0.5mg/kg (0.05, 0.2, 0.5, and 1.0mg/kg for green tea). Mean recoveries were between 71.2% and 113.8%, and intra and inter-day precisions were below 14.8%. The limit of quantitation for 26 matrices ranged from 10 to 50μg/kg. Matrix-matched calibration was used. The method was subsequently applied for real sample analysis, and hymexazol was detected in a cucumber (below the LOQ) and was not detected in any other sample. The method is simple and effective, and meets the routine monitoring requirements for hymexazol residue in foods.
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: jiangzejun55@163.com.
Zejun
Z
Jiang
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: nkylihui@163.com.
Hui
H
Li
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: aicaoxiaolin@126.com.
Xiaolin
X
Cao
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: dupengfei2011@163.com.
Pengfei
P
Du
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: nkshaohua@163.com.
Hua
H
Shao
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: jinfenbj@163.com.
Fen
F
Jin
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: katonking@163.com.
Maojun
M
Jin
Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Research Center of Quality & Standards for Agro-products, Ministry of Agriculture, Beijing 100081, PR China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: w_jing2001@126.com.
Jing
J
Wang
D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D010080:Oxazoles / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010080:Oxazoles; C531211:hymexazol
Acetonitrile (PubChem CID: 6342); Ammonium acetate (PubChem CID: 517165); Foods of plant origin; Formic acid (PubChem CID: 284); Hymexazol (PubChem CID: 24781); Hymexazol residue; LC–MS/MS; Magnesium sulfate (PubChem CID: 24083); Matrix-matched calibration; QuEChERS; Sodium chloride (PubChem CID: 5234)