Upregulation of TSPAN12 is associated with the colorectal cancer growth and metastasis.
Tetraspanin 12 (TSPAN12), as an earliest member of tetraspanin family, has been recently shown to be highly expressed in several malignant tumors, such as lung cancer and breast cancer, which plays an important role in regulating cell proliferation, migration and invasion. However, the functional roles of TSPAN12 in colorectal cancer (CRC) remain largely unclear. In this study, the expression of TSPAN12 was up-regulated compared to that in paracarcinoma tissues. Higher TSPAN12 expression was significantly correlated with TNM stage, tumor size and lymph node metastasis. The vitro functional analysis, including MTT, colony formation, flow cytometry and transwell assays indicated that lentivirus-mediated TSPAN12 knockdown significantly suppressed cell proliferation, migration and invasion, induced cell apoptosis of CRC cells. In addition, knockdown of TSPAN12 remarkably decreased the growth of subcutaneously inoculated tumors in nude mice. Our findings for the first time supported that TSPAN12 might play a positive role in the regulation of CRC cell proliferation, migration and invasion. The inhibition of TSPAN12 may serve as a novel promising therapeutic strategy against human CRC.
Department of Critical Care Medicine, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, China.
Jiao
J
Liu
Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, China.
Chuang
C
Chen
Department of Critical Care Medicine, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, China.
Guang
G
Li
Deparment of Emergency and Critical Care Medicine, Shanghai Changzheng Hospital, Second Military Medical University Shanghai 200003, China.
Dechang
D
Chen
Department of Critical Care Medicine, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, China.
Qingshan
Q
Zhou
Colorectal cancer; TSPAN12; cell proliferation; invasion; shRNA