Roles of Budding Yeast Hrr25 in Recombination and Sporulation.
Hrr25, a casein kinase 1 δ/ε homolog in budding yeast, is essential to set up mono-orientation of sister kinetochores during meiosis. Hrr25 kinase activity coordinates sister chromatid cohesion via cohesin phosphorylation. Here, we investigated the prophase role of Hrr25 using the auxin-inducible degron system and by ectopic expression of Hrr25 during yeast meiosis. Hrr25 mediates nuclear division in meiosis I but does not affect DNA replication. We also found that initiation of meiotic double-strand breaks as well as joint molecule formation were normal inHRR25-deficient cells. Thus, Hrr25 is essential for termination of meiotic division but not homologous recombination.
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Min-Su
MS
Lee
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Jeong Hwan
JH
Joo
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Keunpil
K
Kim
D047389:Casein Kinase I / Q000235:genetics* / Q000378:metabolism*; D048750:Cell Nucleus Division / Q000235:genetics; D020090:Chromosome Segregation; D053903:DNA Breaks, Double-Stranded; D059765:Homologous Recombination; D008540:Meiosis; D011418:Prophase / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D013172:Spores, Fungal / Q000502:physiology
D029701:Saccharomyces cerevisiae Proteins; D047389:Casein Kinase I; C070775:HRR25 protein, S cerevisiae
Hrr25; homologous recombination; meiosis