Effects of strontium ranelate treatment on osteoblasts cultivated onto scaffolds of trabeculae bovine bone.
Blocks of Bovine bone have shown promising results as implantable scaffolds to promote bone regeneration. Strontium ranelate (SrR) is both an antiresorptive and an anabolic drug that has been indicated for oral administration to treat osteoporosis. Few studies, however, have investigated the local effects of SrR and its use in association with biomaterials thus far. In this work, we investigated SrR effects in cultures of primary osteoblasts (PO, from Wistar rats calvaria) and immortalized osteoblasts (IO, from MC3T3-E1 cell line) cultivated as a monolayer or in association with scaffolds of bovine bone in mineralized (MBB) and demineralized (DBB) forms. The optimum dose to induce SrR effects on cell viability was established as 0.1 mM. Our results suggested that the local administration of SrR is biocompatible and non-cytotoxic. In addition, SrR appeared to accelerate primary osteoblast cell differentiation by enhancing alkaline phosphatase activity, the expression of osteogenic differentiation markers, the synthesis of the organic matrix, and a decrease of Ca2+ions in mineralized nodules. DBB was found to be a better scaffold material to promote PO and IO cell proliferation. Exposing the proteins of the demineralized bone matrix might improve scaffold osteoconductive properties. Our results indicated the importance of further investigation of the administration of SrR at sites of bone repair. The association of SrR and bone grafts suggests the possibility of using SrR as a co-adjuvant for bone tissue bioengineering and in bone regeneration therapies.
Instituto de Ciências Biológicas, Departamento de Morfologia, Laboratório de Biologia Oral e do Desenvolvimento, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
Gerluza Aparecida Borges
GAB
Silva
Instituto de Ciências Biológicas, Departamento de Morfologia, Laboratório de Biologia Oral e do Desenvolvimento, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
Bruno Machado
BM
Bertassoli
Instituto de Ciências Biológicas, Departamento de Morfologia, Laboratório de Biologia Oral e do Desenvolvimento, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
Cristiane Aparecida
CA
Sousa
Instituto de Ciências Biológicas, Departamento de Morfologia, Laboratório de Biologia Oral e do Desenvolvimento, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
Juliano Douglas
JD
Albergaria
Instituto de Ciências Biológicas, Departamento de Morfologia, Laboratório de Biologia Oral e do Desenvolvimento, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
Rayan Silva
RS
de Paula
Instituto de Ciências Biológicas, Departamento de Morfologia, Laboratório de Biologia Oral e do Desenvolvimento, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil. erika.cris.jorge@gmail.com.
Erika Cristina
EC
Jorge
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000071556:Cancellous Bone / Q000187:drug effects*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D007477:Ions; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D017208:Rats, Wistar; D013876:Thiophenes / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry*
D007477:Ions; D013876:Thiophenes; C081587:strontium ranelate; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium
Bone engineering; Bovine bone; Osteoblasts; Osteogenesis; Strontium