Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH).
The implementation of quantitative approaches in telomere chromosome-oriented FISH (telomeric CO-FISH) allows the assessment of the relative efficiency of lagging versus leading strand telomere replication and thus provides information on the implicated mechanisms. Here we describe a simple method for telomere strand-specific analyses and discuss its potential applications.
Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d'Ulm, 75248, Paris, France.|UPMC Univ. Paris 06, F-75005, Paris, France.
Isabelle
I
Ourliac-Garnier
Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d'Ulm, 75248, Paris, France.|UPMC Univ. Paris 06, F-75005, Paris, France.
Arturo
A
Londoño-Vallejo
D002875:Chromosomes / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D016615:Telomere / Q000235:genetics*
CO-FISH; FISH; LNA; Lagging; Leading; PNA; Q-CO-FISH; Replication; Telomere