Comparison of Selected Nonlethal Samples from Adult Steelhead for Detection of Infectious Hematopoietic Necrosis Virus Using Cell Culture.
Nonlethal sampling techniques have previously been evaluated for detection of a variety of viral salmonid pathogens. However, many of these studies have used molecular assays in lieu of widely accepted cell culture techniques to evaluate the sampled tissues. Samples were collected from female steelhead Oncorhynchus mykiss broodstock using three potential nonlethal sampling methods (mucus/skin scrape, pectoral fin clip, and gill tissue biopsy) and evaluated for the presence of infectious hematopoietic necrosis virus (IHNV) via cell culture techniques. The results were compared with those from samples collected using a standard lethal sampling method (pooled anterior kidney and spleen tissues) applied to the same fish. Of the three nonlethal sampling techniques that were evaluated, fin clipping was the easiest and least invasive method. Furthermore, fin tissue was as sensitive as or more sensitive than kidney/spleen tissue for detecting IHNV in this population of fish. However, with the exception of gill tissue, the nonlethal samples did not appear to be appropriate surrogates for lethally collected tissues with regard to identifying an active infection in a particular fish. Nevertheless, nonlethal sampling coupled with cell culture appears to be suitable for helping to define the IHNV status of a steelhead population. Received July 27, 2016; accepted December 11, 2016.
a Pacific States Marine Fisheries Commission, Eagle Fish Health Laboratory , 1800 Trout Road, Eagle , Idaho 83616 , USA.
David R
DR
Burbank
b Idaho Department of Fish and Game , Eagle Fish Health Laboratory , 1800 Trout Road, Eagle , Idaho 83616 , USA.
Tyson R
TR
Fehringer
b Idaho Department of Fish and Game , Eagle Fish Health Laboratory , 1800 Trout Road, Eagle , Idaho 83616 , USA.
Luciano V
LV
Chiaramonte
D000818:Animals; D002478:Cells, Cultured / Q000821:virology; D005260:Female; D005393:Fish Diseases / Q000175:diagnosis*; D005880:Gills; D029142:Infectious hematopoietic necrosis virus / Q000302:isolation & purification*; D017686:Oncorhynchus mykiss; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary*