Molecular Characterization and Antioxidant Potential of Three Wild Culinary-Medicinal Mushrooms from Tripura, Northeast India.
The aim of this study was to characterize 3 wild culinary-medicinal mushrooms using molecular tools and to analyze their antioxidant activity. Antioxidant properties were studied by evaluating free radical scavenging, reducing power, and chelating effect. The mushrooms were identified as Lentinus squarrosulus, L. tuber-regium, and Macrocybe gigantean by amplifying internal transcribed spacer regions of ribosomal DNA. The results demonstrated that the methanolic extract of M. gigantean has the highest free radical scavenging effect and chelating effect, whereas the methanolic extract of L. squarrosulus has the highest reducing power. The highest total phenol content and the most ascorbic acid were found in the M. gigantean extracts. Among the 3 mushroom extracts, M. gigantean displayed the most potent antioxidant activity. Molecular characterization using the nuclear ribosomal internal transcribed spacer region as a universal DNA marker was an effective tool in the identification and phylogenetic analysis of the studied mushrooms. The study also indicated that these wild macrofungi are rich sources of natural antioxidants.
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India.
Aparajita Roy
AR
Das
Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya, India.
Madhusmita
M
Borthakur
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India.
Ajay Krishna
AK
Saha
Microbiology Laboratory, Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong-793022, Meghalaya, India.
Santa Ram
SR
Joshi
Microbiology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India.
Panna
P
Das
D000363:Agaricales / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D016166:Free Radical Scavengers / Q000032:analysis*; D007194:India; D010636:Phenols / Q000032:analysis; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D000975:Antioxidants; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D016166:Free Radical Scavengers; D010636:Phenols; D001205:Ascorbic Acid