Anti-oxidative Responses on Hepatic Tissue of Zebrafish (Danio rerio) in a Short Duration of Sub-lethal Concentrations of Cadmium Exposure.
The aim of the present study was to identify whether the responses of oxidative stress in zebrafish liver are similar to those in mammalians upon low doses of Cd2+exposure in short durations. Fish were exposed to 1.78 μM Cd2+(treatment) and 0.0 μM Cd2+(control) for 0, 1, 3, and 6 h. The reactive oxygen species (ROS) and lipid peroxidation (LPO) of hepatic tissues significantly increased after 3 and 6 h of Cd2+exposure, respectively. Antioxidants glutathione peroxidase (gpx1a), superoxide dismutase (sod), and catalase (cat) were up regulated after 1-3 h, and metallothionein isoforms (smtB and mt2) increased after 3-6 h of Cd2+exposure. The caspase-3 and p53 mRNA expressions significantly increased threefolds after 1 h of Cd2+exposure. Results confirmed that oxidative stress in the hepatic tissue was induced by Cd2+within 3 h. However, anti-oxidative functions immediately up regulated, causing cell apoptosis levels to decrease after 6 h of Cd2+exposure.
Department of Aquatic Biosciences, National Chiayi University, Chiayi, 600, Taiwan, Republic of China. sumei@mail.ncyu.edu.tw.
Su Mei
SM
Wu
Department of Aquatic Biosciences, National Chiayi University, Chiayi, 600, Taiwan, Republic of China.
Li-Hsin
LH
Shu
Department of Aquatic Biosciences, National Chiayi University, Chiayi, 600, Taiwan, Republic of China.
Jia-Hao
JH
Liu
Graduate Institute of Biomedical and Biopharmaceutical Sciences, National Chiayi University, Chiayi, 600, Taiwan, Republic of China.
Ching Hsein
CH
Chen
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D002104:Cadmium
Apoptosis; Cadmium; Metallothionein; Oxidative stress; Zebrafish