Production of bioethanol from Napier grass via simultaneous saccharification and co-fermentation in a modified bioreactor.
The aim of this study was to use a modified bioreactor system for simultaneous saccharification of cellulose and bioethanol production. We tested Aspergillus niger and Trichoderma reesei for cellulose saccharification and Zymomonas mobilis for bioethanol production simultaneously in this modified bioreactor. The results showed that various carboxymethylcellulose (CMC) concentrations (10, 15, or 20 g/L) as a substrate for A. niger and T. reesei yielded bioethanol production of 0.51, 0.78, and 0.89 g/L and a CMC conversion rate of 10.2%, 10.7%, and 8.89%, respectively. These data suggested that at 10 g/L CMC as a substrate, the CMC conversion rate was higher than that at the other concentrations. When CMC concentration exceeded 15 g/L, bioethanol production was prolonged to 40 h. These results were attributed to the viscosity of CMC. This study also tested Napier grass (an agricultural byproduct) for bioethanol production. The results revealed bioethanol production and the theoretical Napier grass conversion rate were 0.38 g/L and 12.6%, respectively, for 13-h cultivation when the feeding concentration of Napier grass was 10 g/L. When Napier grass concentration was increased to 15 g/L, bioethanol production and the Napier grass conversion rate reached 0.51 g/L and 23%, respectively, after 14-h cultivation. Eventually, the experimental results indicated using agricultural waste for bioethanol production has been become a potential strategy.
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., TaoYuan City 33302, Taiwan, ROC.
Yu-Kuo
YK
Liu
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, No. 135 Yuan-Tung Road, Chung-Li Dist., Taoyuan City 32003, Taiwan, ROC.
Wei-Chuan
WC
Chen
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, No. 135 Yuan-Tung Road, Chung-Li Dist., Taoyuan City 32003, Taiwan, ROC.
Yu-Ching
YC
Huang
Department & Graduate Institute of Chemical Engineering & Graduate Institute of Biochemical Engineering, Ming Chi University of Technology, No. 84 Gungjuan Rd, Taishan Dist., New Taipei City 24301, Taiwan, ROC.
Yu-Kaung
YK
Chang
Department of Chemical Engineering, National Tsing Hwa University, No. 101 Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan, ROC.
I-Ming
IM
Chu
Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43 Keelung Rd., Sec. 4, Da'an Dist., Taipei City 10607, Taiwan, ROC.
Shen-Long
SL
Tsai
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, No. 135 Yuan-Tung Road, Chung-Li Dist., Taoyuan City 32003, Taiwan, ROC. Electronic address: yhwei@saturn.yzu.edu.tw.
Yu-Hong
YH
Wei
D001234:Aspergillus niger / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D002482:Cellulose / Q000378:metabolism; D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D031782:Pennisetum / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D014242:Trichoderma / Q000378:metabolism*; D017355:Zymomonas / Q000378:metabolism*
D000431:Ethanol; D002482:Cellulose
Bioethanol; Carboxymethylcellulose; Modified bioreactor; Napier grass; Saccharification