Ratiometric Visualization of NO/H2S Cross-Talk in Living Cells and Tissues Using a Nitroxyl-Responsive Two-Photon Fluorescence Probe.
It is of scientific significance to explore the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (H2S) because they exert similar and interdependent biological actions within the living organisms. Nevertheless, visualization of the NO/H2S crosstalk using effective molecular imaging tools remains challenging. To address this issue, and given that nitroxyl (HNO) has been implicated as the interdependent production of NO and H2S via a network of cascading chemical reactions, we herein design a ratiometric two-photon fluorescent probe for HNO, termed TP-Rho-HNO, which consists of benzo[h]chromene-rhodol scaffold as two-photon energy transfer cassette with phosphine moiety as specific HNO recognition unit. The newly proposed probe has been successfully applied in ratiometric two-photon bioimaging of endogenous HNO derived from NO and H2S interaction in the human umbilical vein cells (HUVECs) and as well as in rat brain tissues. Intriguingly, the imaging results consistently demonstrate that the mutually dependent upgeneration of H2S and NO are present in living biosystems, indicating that this molecular probe would provide a powerful approach to elucidate the chemical foundation for the anfractuous cross-talk between the NO and H2S signaling pathways in biology.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, P. R. China.
Yibo
0000-0003-1862-9926
Y
Zhou
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, P. R. China.
Xiufang
0000-0001-7837-8167
X
Zhang
Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha 410114, P. R. China.
Sheng
0000-0003-0867-6438
S
Yang
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, P. R. China.
Yuan
0000-0001-9312-0885
Y
Li
Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha 410114, P. R. China.
Zhihe
0000-0002-6268-225X
Z
Qing
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, P. R. China.
Jing
0000-0003-1747-5398
J
Zheng
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, P. R. China.
Jishan
0000-0001-8144-361X
J
Li
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, P. R. China.|Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha 410114, P. R. China.
Ronghua
0000-0001-7873-6892
R
Yang
D000818:Animals; D001921:Brain / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry* / Q000378:metabolism; D036641:Microscopy, Fluorescence, Multiphoton; D009569:Nitric Oxide / Q000737:chemistry* / Q000378:metabolism; D009589:Nitrogen Oxides / Q000737:chemistry*; D051381:Rats; D044004:Xanthones / Q000737:chemistry
D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; D044004:Xanthones; C000607488:rhodol; D009569:Nitric Oxide; C039900:nitroxyl; D006862:Hydrogen Sulfide