On the potential of using peculiarities of the protein intrinsic disorder distribution in mitochondrial cytochromebto identify the source of animal meats.
This study was conducted to identify the source of animal meat based on the peculiarities of protein intrinsic disorder distribution in mitochondrial cytochromeb(mtCyt-b). The analysis revealed that animal and avian species can be discriminated based on the proportions of the two groups of residues, Leu+Ile, and Ser+Pro+Ala, in the amino acid sequences of their mtCyt-b. Although levels of the overall intrinsic disorder in mtCyt-bis not very high, the peculiarities of disorder distribution within the sequences of mtCyt-bfrom different species varies in a rather specific way. In fact, positions and intensities of disorder/flexibility "signals" in the corresponding disorder profiles are relatively unique for avian and animal species. Therefore, it is possible to devise a set of simple rules based on the peculiarities of disorder profiles of their mtCyt-bproteins to discriminate among species. This intrinsic disorder-based analysis represents a new technique that could be used to provide a promising solution for identification of the source of meats.
Department of Biological Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia; Department of Cell Biology, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Gizza, Egypt.
Haitham A
HA
Yacoub
Department of Biological Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia; EgyBlood, Vacsera, Cairo, Egypt.
Mahmoud A
MA
Sadek
Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia; Department of Molecular Medicine and USF Health Byrd Alzheimer Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Vladimir N
VN
Uversky
adulteration; animal species; authentication; avian species; food products; intrinsically disordered protein; intrinsically disordered region; meat; mitochondrial cytochrome b