First evidences of interaction between pyranoanthocyanins and salivary proline-rich proteins.
The contribution of other classes of polyphenol compounds besides tannins to the overall perception of astringency is still poorly understood. So, this work aimed to study the interaction between a family of salivary proline-rich proteins (aPRPs) and representative pyranoanthocyanins in red wines [pyranomalvidin-3-glucoside (vitisin B), pyranomalvidin-3-glucoside-catechol, and pyranomalvidin-3-glucoside-epicatechin] using saturation transfer difference-NMR and MALDI-TOF. For vitisin B KDwas of 1.74mM; for pyranomalvidin-3-glucoside-catechol was 1.17mM and for pyranomalvidin-3-glucoside-epicatechin it was 0.87mM. The presence of the flavanol structural unit in the pyranoanthocyanins led to an increase in their interaction with aPRPs. Further, it is also interesting that the values obtained were in the range of KDobtained previously reported for the interaction between the human saliva proline-rich peptides (IB714and IB937) and procyanidins. Overall, the results obtained suggest that, along with tannins, other polyphenols present in red wine, namely pyranoanthocyanins, could actively contribute to red wine global astringency.
REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal. Electronic address: ignacio.estevez@fc.up.pt.
Ignacio
I
García-Estévez
REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal. Electronic address: luis.cruz@fc.up.pt.
Luís
L
Cruz
REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal. Electronic address: jsoliveira@fc.up.pt.
Joana
J
Oliveira
REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal. Electronic address: nbmateus@fc.up.pt.
Nuno
N
Mateus
REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal. Electronic address: vfreitas@fc.up.pt.
Victor
V
de Freitas
REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal. Electronic address: susana.soares@fc.up.pt.
Susana
S
Soares
D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D055273:Salivary Proline-Rich Proteins / Q000737:chemistry / Q000378:metabolism*
D000872:Anthocyanins; D055273:Salivary Proline-Rich Proteins
Acidic proline-rich proteins; Interaction; MALDI-mass spectrometry; Pyranoanthocyanins; STD-NMR