Overexpression of Drosha, DiGeorge syndrome critical region gene 8 (DGCR8), and Dicer mRNAs in the pathogenesis of psoriasis.
BACKGROUND
Psoriasis is a complex autoimmune inflammatory disease that occurs in genetically susceptible individuals and presents with the development of inflammatory plaques on the skin. Recent studies have indicated that microRNAs (miRNAs) play important roles in psoriasis.


OBJECTIVE
To investigate whether expression of Drosha, DGCR8, and Dicer mRNAs is involved in the pathogenesis of psoriasis.


METHODS
Biopsies were obtained from involved psoriatic skin (PP), noninvolved psoriatic skin (PN), and healthy skin (NN). Expression of Drosha, Dicer, and DGCR8 was assessed with real-time quantitative real-time PCR in 25 patients with psoriasis and 25 healthy volunteers.


RESULTS
We observed that expression levels of Drosha, Dicer, and DGCR8 were upregulated in patients with psoriasis compared to the control group. However, the Drosha and Dicer expression levels were higher in PP tissues and PN tissues compared to NN tissues, but they were more upregulated in PP tissues compared to PN tissues (P<.001). Although the DGCR8 expression was higher in PP tissues and PN tissues compared to NN tissues, it was more upregulated in PN tissues compared to PP tissues (P<.001).


CONCLUSIONS
Our data demonstrate that upregulated expression of Drosha, DGCR8, and Dicer mRNAs may be involved in the pathogenesis of psoriasis.
Department of Dermatology, Imam Khomeini Hospital, Ardebil University of Medical Sciences, Ardabil, Iran.
Majid
M
Rostami Mogaddam
Department of Midwifery, Ardebil Branch, Islamic Azad University, Ardebil, Iran.
Nastaran
N
Safavi Ardabili
Department of Surgery, Imam Khomeini Hospital, Ardebil University of Medical Sciences, Ardebil, Iran.
Yousef
Y
Shafaeei
Department of Endocrine and Metabolic Diseases, The Persian Gulf Tropical Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.
Nasrollah
http://orcid.org/0000-0003-1511-7052
N
Maleki
Markey Cancer Center, University of Kentucky, Lexington, USA.
Naser
N
Jafari
Department of Dermatology, Imam Khomeini Hospital, Ardebil University of Medical Sciences, Ardabil, Iran.
Alireza
A
Jafari
D000328:Adult; D016022:Case-Control Studies; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics*; D043244:Ribonuclease III / Q000235:genetics*; D012867:Skin / Q000378:metabolism; D015854:Up-Regulation
C474043:DGCR8 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C408087:DICER1 protein, human; C517918:DROSHA protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
biopsy; microRNAs; psoriasis; skin; upregulation