KRAS and VEGF gene 3'-UTR single nucleotide polymorphisms predicted susceptibility in colorectal cancer.
Single nucleotide polymorphisms (SNPs) in tumor-related genes have been reported to play important roles in cancer development. Recent studies have shown that 3'-untranslated regions (UTR) polymorphisms are associated with the occurrence and prognosis of cancers. The aim of this study is to analyze the association between KRAS and VEGF gene 3'-UTR SNPs and genetic susceptibility to colorectal cancer (CRC). In this case-control study of 371 CRC cases and 246 healthy controls, we analyzed the association between one SNP (rs1137188G > A) in the KRAS gene and four SNPs (rs3025039C > T, rs3025040C > T, rs3025053G > A and rs10434A > G) in the VEGF gene and CRC susceptibility by the improved multiplex ligase detection reaction (iMLDR) method. We checked the selected SNPs' minor allele frequency and its distribution in the frequency of Chinese people by Hap-map database and Hardy-Weinberg equilibrium, and used multivariate logistic regression models to estimate adjusted odds ratios (AORs) and 95% confidence intervals (95% CIs). We found that the rs3025039C variant genotype in the VEGF gene was associated with a significant protection for CRC (AOR = 0.693, 95% CI = 0.485-0.989; P = 0.043 for CC and CT+TT). Nevertheless, the difference was no longer significant after Bonferroni correction (Bonferroni-adjusted P = 0.172). In genetic polymorphisms analysis, we found that the KRAS rs1137188 variant AA genotype had higher portion of tumor size (≥ 5 cm) (P = 0.01; Bonferroni-adjusted P = 0.04), which suggested that the rs1137188 variant AA genotype may significantly be associated with increased progression of CRC. In conclusion, our study suggested that these five SNPs in the KRAS gene and the VEGF gene were not associated with CRC susceptibility in Han Chinese in Sichuan province.
College of Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China.
Minnan
M
Yang
Department of Pathology, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
Xiuli
X
Xiao
College of Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China.
Xiaorui
X
Xing
Department of Gastrointestinal surgery, Chengdu University of Traditional Chinese Medicine Affiliated Hospital, Chengdu, Sichuan, China.
Xin
X
Li
Department of Pathology, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
Tian
T
Xia
Department of Pathology, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.|Department of Science and Technology, Southwest Medical University, Luzhou, Sichuan, China.
Hanan
H
Long
D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D055815:Young Adult
D020413:3' Untranslated Regions; C117307:KRAS protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016283:Proto-Oncogene Proteins p21(ras)