RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape.
Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 (SHP1). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv. SLM046. Molecular analysis of T2 transgenic plants by RT-PCR and Real-time PCR showed that expression of the BnSHP alleles was highly decreased in comparison with control plants. Morphologically, transgenic plants were normal and produced seeds at greenhouse conditions. At ripening, stage pods failed to shatter, and a finger pressure was needed for pod opening.
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.|Ramin University of Agricultural and Natural Resources, Ahvaz, Iran.
Hadis
H
Kord
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran. a_shakib@abrii.ac.ir.
Ali Mohammad
AM
Shakib
Ramin University of Agricultural and Natural Resources, Ahvaz, Iran.
Mohammad Hossein
MH
Daneshvar
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Pejman
P
Azadi
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Vahid
V
Bayat
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Mohsen
M
Mashayekhi
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Mahboobeh
M
Zarea
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Alireza
A
Seifi
Department of Tissue culture and Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Mana
M
Ahmad-Raji
BnSHP gene; Gene silencing; Oilseed rape; Pod shattering; RNAi