Layer-by-layer self-assembly of gold nanoparticles/thiols β-cyclodextrin coating as the stationary phase for enhanced chiral differentiation in open tubular capillary electrochromatography.
The popularity of capillary electrochromatography (CEC) has led to an increasing number of studies on the development and evaluation of enantioselective CEC systems. Herein, a novel, simple, and economical method for the preparation of chiral stationary phases for enantioselective open tubular capillary electrochromatography (OTCEC) was reported for the first time. This novel capillary column was fabricated through layer-by-layer self-assembly of GNPs on a 3-mercaptopropyl-trimethoxysilane (MPTMS)-modified fused-silica capillary and subsequent surface functionalization of the GNPs through self-assembly of thiols β-cyclodextrin (SH-β-CD). With this method, monolayer and multilayer GNPs film capillary columns were prepared and evaluated using meptazinol and its three intermediate enantiomers as templates. Consequently, the three-layer GNPs capillary column was found to exhibit favorable enantioseparation performance. Factors that influence the chiral separation resolution were examined. Under the optimized conditions, enantioselectivity was obtained for meptazinol and its three intermediate enantiomers (intermediates Ⅱ, Ⅲ and Ⅳ) in less than 20min with resolutions of 2.05, 0.36, 1.67, 1.53, respectively. The proposed column revealed adequate repeatability concerning run-to-run and day-to-day. In brief, these results confirmed the use of GNPs as the electrochromatographic support can enhance the phase ratio of OTC column in capillary electrochromatography (CEC). Then, this proposed method was well validated with good linearity (≥0.999), recovery (92.0-94.5%) and repeatability, and was successfully used for enantioseparation of meptazinol in spiked urine samples, which indicated the new column's potential usage in biological analysis.
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, PR China.
Lin-Lin
LL
Fang
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, PR China.
Ping
P
Wang
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, PR China.
Xiao-Li
XL
Wen
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, PR China.
Xin
X
Guo
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, PR China.
Lin-da
LD
Luo
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, PR China. Electronic address: Yujia_yc@163.com.
Jia
J
Yu
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, PR China. Electronic address: gxjhyz@gmail.com.
Xing-Jie
XJ
Guo
D053801:Capillary Electrochromatography / Q000379:methods*; D005233:Fatty Alcohols / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D005233:Fatty Alcohols; D013438:Sulfhydryl Compounds; D047392:beta-Cyclodextrins; C000591091:thiol-beta-cyclodextrin; C118975:1,10-decanediol; D006046:Gold; D012822:Silicon Dioxide
Chiral stationary phase; Enantioseparation; Gold nanoparticles; Open tubular capillary electrochromatography