Injectable nanoparticles/hydrogels composite as sustained release system with stromal cell-derived factor-1α for calvarial bone regeneration.
Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton. Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy. In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels. The SDF-1α encapsulated in CS/CMCS NPs within CS/GP hydrogels showed significantly sustained release effect. The cumulative release of SDF-1α was only 40% during 28d. The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01). Histological data also confirmed this difference. This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
Department of Preventive Dentisty, The Research Center of Stomatology, Stomatology Hospital, Xi'an Jiaotong University, No. 98 West-Five Road, Xi'an 710004, China; Department of Stomatology, Yulin First Hospital, No. 93 Yuxi Avenue, Yulin, 719000 Shaanxi Province, China.
Lei
L
Mi
Department of Stomatology, Yulin First Hospital, No. 93 Yuxi Avenue, Yulin, 719000 Shaanxi Province, China.
Huaiqin
H
Liu
Department of Stomatology, Yulin First Hospital, No. 93 Yuxi Avenue, Yulin, 719000 Shaanxi Province, China.
Yu
Y
Gao
Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
Hui
H
Miao
Department of Preventive Dentisty, The Research Center of Stomatology, Stomatology Hospital, Xi'an Jiaotong University, No. 98 West-Five Road, Xi'an 710004, China. Electronic address: ruanjianping501@163.com.
Jianping
J
Ruan
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D054377:Chemokine CXCL12 / Q000737:chemistry*; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry; D003692:Delayed-Action Preparations; D065546:Drug Liberation; D005990:Glycerol / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D055114:X-Ray Microtomography
D001672:Biocompatible Materials; D054377:Chemokine CXCL12; D003692:Delayed-Action Preparations; D020100:Hydrogels; C514968:carboxymethyl-chitosan; D048271:Chitosan; D005990:Glycerol
Bone regeneration; Chitosan; Hydrogels; Nanoparticles; Stromal cell-derived factor-1α