M protein is sufficient for assembly and release of Peste des petits ruminants virus-like particles.
Peste des petits ruminants virus (PPRV), belonging to paramyxoviruses, has six structure proteins (such as matrix protein (M), nucleocapsid proteins (N), fusion protein (F) and hemagglutinin protein (H)) and could cause high morbidity and mortality in sheep and goats. Although a vaccine strain of PPRV has been rescued and co-expression of M and N could yield PPRV-like particles, the roles of structure proteins in virion assembly and release have not been investigated in detail. In this study, plasmids carrying PPRV cDNA sequences encoding the N, M, H, and F proteins were expressed in Vero cells. The co-expression of all four proteins resulted in the release of virus-like particles (VLPs) with similar release efficiency to that of authentic virions. Moreover, the co-expression of M together with F also resulted in efficient VLPs release. In the absence of M protein, the expression of no combination of the other proteins resulted in particle release. In summary, a VLPs production system for PPRV has been established and M protein is necessary for promoting the assembly and release of VLPs, of which the predominant protein is M protein. Further study will be focused on the immunogenicity of the VLPs.
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Qiuxia
Q
Wang
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
Changbo
C
Ou
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Yongxi
Y
Dou
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Lei
L
Chen
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Xuelian
X
Meng
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
Xingyou
X
Liu
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
Yan
Y
Yu
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
Jinqing
J
Jiang
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
Jinyou
J
Ma
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Zhidong
Z
Zhang
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
Jianhe
J
Hu
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China; China Institute of Veterinary Drugs Control, Beijing 100000, China. Electronic address: caixp@vip.163.com.
Xuepeng
X
Cai
D000818:Animals; D000914:Antibodies, Viral; D002522:Chlorocebus aethiops / Q000378:metabolism / Q000502:physiology; D018076:DNA, Complementary; D004279:DNA, Viral; D006389:Hemagglutinins, Viral / Q000378:metabolism / Q000502:physiology; D051379:Mice; D019590:Nucleocapsid Proteins / Q000378:metabolism / Q000502:physiology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000502:physiology*; D014709:Vero Cells / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000378:metabolism / Q000502:physiology; D014763:Viral Matrix Proteins / Q000378:metabolism*
D000914:Antibodies, Viral; D018076:DNA, Complementary; D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D019590:Nucleocapsid Proteins; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins
Assembly; M protein; Peste des petits ruminants virus; Release; Virus-like particles