Hepatoprotective Effects ofAntrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol-Induced Acute Liver Injury.
In the present study, the components ofA. cinnamomea(AC) mycelia were systematically analyzed. Subsequently, its hepatoprotective effects and the underlying mechanisms were explored using a mouse model of acute alcohol-induced liver injury. AC contained 25 types of fatty acid, 16 types of amino acid, 3 types of nucleotide, and 8 types of mineral. The hepatoprotective effects were observed after 2 weeks of AC treatment at doses of 75 mg/kg, 225 mg/kg, and 675 mg/kg in the mouse model. These effects were indicated by the changes in the levels of aspartate aminotransferase, alanine aminotransferase, several oxidation-related factors, and inflammatory cytokines in serum and/or liver samples. AC reduced the incidence rate of necrosis, inflammatory infiltration, fatty droplets formation, and cell apoptosis in liver detecting via histological and TUNEL assay. In addition, AC reduced the expression of cleaved caspase-3, -8, and -9 and the levels of phosphor-protein kinase B (Akt) and phosphor-nuclear factor-κB (NF-κB) in the liver samples. Collectively, AC-mediated hepatoprotective effects in a mouse model of acute alcohol-induced liver injury are the result of reduction in oxidative stress. This may be associated with Akt/NF-κB signaling. These results provide valuable evidence to support the use ofA. cinnamomeaas a functional food and/or medicine.
School of Life Sciences, Jilin University, Changchun 130012, China.
Yange
Y
Liu
School of Life Sciences, Jilin University, Changchun 130012, China.
Juan
J
Wang
School of Life Sciences, Jilin University, Changchun 130012, China.
Lanzhou
L
Li
School of Life Sciences, Jilin University, Changchun 130012, China.
Wenji
W
Hu
School of Life Sciences, Jilin University, Changchun 130012, China.
Yidi
Y
Qu
School of Life Sciences, Jilin University, Changchun 130012, China.
Yipei
Y
Ding
School of Life Sciences, Jilin University, Changchun 130012, China.
Lina
L
Meng
School of Life Sciences, Jilin University, Changchun 130012, China; Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China.
Lirong
0000-0003-1324-877X
L
Teng
School of Life Sciences, Jilin University, Changchun 130012, China; Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China.
Di
0000-0002-7322-4174
D
Wang
D000410:Alanine Transaminase / Q000097:blood; D000438:Alcohols / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D055491:Antrodia / Q000737:chemistry* / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
D000438:Alcohols; D000975:Antioxidants; D016207:Cytokines; D016328:NF-kappa B; D010936:Plant Extracts; D020011:Protective Agents; D017382:Reactive Oxygen Species; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9