Enzyme IIANtrRegulates Salmonella Invasion Via 1,2-Propanediol And Propionate Catabolism.
Many Proteobacteria possess a nitrogen-metabolic phosphotransferase system (PTSNtr) consisting of EINtr, NPr, and EIIANtr(encoded by ptsP, ptsO, and ptsN, respectively). The PTSNtrplays diverse regulatory roles, but the substrate phosphorylated by EIIANtrand its primary functions have not yet been identified. To comprehensively understand the roles of PTSNtrin Salmonella Typhimurium, we compared the whole transcriptomes of wild-type and a ΔptsN mutant. Genome-wide RNA sequencing revealed that 3.5% of the annotated genes were up- or down-regulated by three-fold or more in the absence of EIIANtr. The ΔptsN mutant significantly down-regulated the expression of genes involved in vitamin B12synthesis, 1,2-propanediol utilization, and propionate catabolism. Moreover, the invasiveness of the ΔptsN mutant increased about 5-fold when 1,2-propanediol or propionate was added, which was attributable to the increased stability of HilD, the transcriptional regulator of Salmonella pathogenicity island-1. Interestingly, an abundance of 1,2-propanediol or propionate promoted the production of EIIANtr, suggesting the possibility of a positive feedback loop between EIIANtrand two catabolic pathways. These results demonstrate that EIIANtris a key factor for the utilization of 1,2-propanediol and propionate as carbon and energy sources, and thereby modulates the invasiveness of Salmonella via 1,2-propanediol or propionate catabolism.
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Woongjae
W
Yoo
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Dajeong
D
Kim
Department of Molecular Science and Technology, Department of Applied Chemistry and Biological Engineering, Ajou University, Suwon 16499, Korea.
Hyunjin
H
Yoon
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.|Institute of Food Industrialization, Institute of Green Bio Science &Technology, Seoul National University, Pyeongchang, Gangwon, Korea.
Sangryeol
S
Ryu
D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D053858:Metabolic Networks and Pathways; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism*; D019946:Propylene Glycol / Q000378:metabolism*; D012475:Salmonella / Q000472:pathogenicity / Q000502:physiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D059467:Transcriptome; D037521:Virulence Factors
D011422:Propionates; D037521:Virulence Factors; D019946:Propylene Glycol; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System