Ginkgo biloba extract EGb761 attenuates brain death-induced renal injury by inhibiting pro-inflammatory cytokines and the SAPK and JAK-STAT signalings.
This study aimed to investigate the protective effects of EGb761, a Ginkgo Biloba extract, against brain death-induced kidney injury. Sixty male Sprague Dawley rats were randomly divided into six groups: sham, brain-death (BD), BD + EGb b48h (48 hours before BD), BD + EGb 2 h (2 hours after BD), BD + EGb 1 h, and BD + EGb 0.5 h. Six hours after BD, serum sample and kidney tissues were collected for analyses. The levels of blood urea nitrogen (BUN) and serum creatinine significantly elevated in the BD group than in sham group. In all the EGb761-treated BD animals except for the BD + Gb 2 h group, the levels of BUN and serum creatinine significantly reduced (all P < 0.01). EGb761 attenuated tubular injury and lowered the histological score. In addition, the longer duration of drug treatment was, the better protective efficacy could be observed. EGb761 significantly reduced IL-1β, IL-6, TNF-α, MCP-1, IP-10 mRNA expression and macrophage infiltration in the kidney. EGb761 treatment at 48 hour before brain death significantly attenuate the levels of p-JNK-MAPK, p-p38-MAPK, and p-STAT3 proteins (all P < 0.05, compared to BD group). In summary, our data showed that EGb761 treatment protected donor kidney from BD-induced damages by blocking SAPK and JAK-STAT signalings. Early administration of EGb761 can provide better protective efficacy.
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.|Organ Transplant Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Yifu
Y
Li
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Yunyi
Y
Xiong
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Huanxi
H
Zhang
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Jun
J
Li
Department of Clinical Laboratory, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Dong
D
Wang
Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Wenfang
W
Chen
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Xiaopeng
X
Yuan
Laboratory Animal Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Qiao
Q
Su
Laboratory Animal Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Wenwen
W
Li
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Huiting
H
Huang
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Zirong
Z
Bi
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Longshan
L
Liu
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, People's Republic of China.|Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Guangzhou 510080, People's Republic of China.|Institution of Organ Donation and Transplant Immunology, Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
Changxi
C
Wang
D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001926:Brain Death / Q000097:blood / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D020441:Ginkgo biloba; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects*; D020935:MAP Kinase Signaling System; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050791:STAT Transcription Factors / Q000378:metabolism; D014019:Tissue Donors; D019737:Transplants / Q000473:pathology / Q000592:standards*; D000096983:Ginkgo Extract
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010936:Plant Extracts; D050791:STAT Transcription Factors; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract