Increased Oxidative Stress in Living Kidney Donors: Correlation of Renal Functions With Antioxidant Capacity.
BACKGROUND
Substantial attention has recently been paid to the possibility of an increased risk of chronic kidney disease (CKD) in living kidney donors. It has been demonstrated that CKD patients suffer from increased oxidative stress, which have been reported to show a strong association with several clinical problems such as accelerated atherosclerosis. The purpose of the current cross-sectional, single-center study was to evaluate the relationship between renal functions of living kidney donors and systemic oxidative stress.


METHODS
A total of 55 living kidney donors operated at least 1 year ago and 40 age- and sex-matched healthy individuals were enrolled in this study. Plasma malondialdehyde (MDA) levels were determined as oxidative stress marker. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured as antioxidants. Renal function parameters and proteinuria were also assessed.


RESULTS
Mean serum creatinine levels were higher (P = .022) and 24-hour creatinine clearance was lower (P = .016) in living kidney donors compared with healthy controls. Serum MDA levels were higher (P = .034), and SOD and GPx activities were lower (P = .023 and P < .001, respectively). There was a significant positive correlation between serum GPx activity and 24-hour creatinine clearance levels (r = 0.524, P < .01). A linear regression analysis showed that serum GPx activity was still significantly and positively correlated with creatinine clearance (regression coefficient = 0.416, P < .001).


CONCLUSIONS
Our data demonstrated that living kidney donors exhibit slightly reduced kidney function, increased oxidative stress, and decreased antioxidant activity. We propose that 24-hour creatinine clearance is positively correlated with antioxidant enzyme GPx. To our knowledge, this is the first study to demonstrate the association between renal functions and antioxidant activity in kidney donors.
Department of Transplantation, Kartal Training Hospital, Istanbul, Turkey.
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Yildirim
Department of Surgery, Kartal Training Hospital, Istanbul, Turkey.
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Karahan
Department of Transplantation, Kartal Training Hospital, Istanbul, Turkey.
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Kucuk
Department of Transplantation, Kartal Training Hospital, Istanbul, Turkey.
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Demir
Department of Biochemistry, Yuzuncu Yil University, Van, Turkey.
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Demir
Department of Biochemistry, Yuzuncu Yil University, Van, Turkey.
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Turan
Department of Nephrology, Tekirdag State Hospital, Tekirdag, Turkey.
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Yavuz
Department of Nephrology, Kartal Training Hospital, Istanbul, Turkey. Electronic address: elifaribakir@gmail.com.
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Ari
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D019520:Living Donors; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D011183:Postoperative Complications / Q000378:metabolism; D051436:Renal Insufficiency, Chronic / Q000209:etiology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase