Identification, characterization and modulation of ferritin-H in the sub-Antarctic Notothenioid Eleginops maclovinus challenged with Piscirickettsia salmonis.
Ferritin is a major iron storage protein essential not only in the infectious process, but also in any circumstance generating oxidative stress. In this study, the cDNA coding sequence of ferritin-H was obtained from the sub-Antarctic Notothenioid fish Eleginops maclovinus through transcriptomic analysis of the head kidney. This sequence contained a 534 bp open reading frame that coded for a 177 amino acid protein with a molecular weight of 20,786.2 Da and a theoretical pI of 5.56. The protein displayed a region of iron putative response elements in the 5'UTR, two putative ferritin iron-binding region signatures, and seven characteristic amino acids with ferroxidase functions. Phylogenetic analysis related this sequence to ferritin-H sequences of other Antarctic Notothenioid fish, sharing 96.61% similarity. Constitutive gene expression analysis in different organs revealed increased ferritin-H gene expression in the gills, spleen, muscle, and liver. After infection with two bacterial strains of Piscirickettsia salmonis (LF-89 and Austral-005), ferritin-H was differentially expressed depending on bacterial strain and tissue. This study provides relevant information towards understanding the iron metabolism of a sub-Antarctic Notothenioid fish.
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Av. Los Pinos s/n Balneario Pelluco, Puerto Montt, Chile; Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile. Electronic address: danixapamela@gmail.com.
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Martínez
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Av. Los Pinos s/n Balneario Pelluco, Puerto Montt, Chile; Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile.
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Oyarzún
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Programa de Magister en Microbiología, Universidad Austral de Chile, Valdivia, Chile; Centro Fondap Interdisciplinary Center for Aquaculture Research (INCAR), Universidad Austral de Chile, Valdivia, Chile.
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Vargas-Lagos
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile.
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Pontigo
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile.
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Soto-Dávila
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Av. Los Pinos s/n Balneario Pelluco, Puerto Montt, Chile.
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J
Saravia
Centro Fondap Interdisciplinary Center for Aquaculture Research (INCAR), Universidad Austral de Chile, Valdivia, Chile; Instituto de Patología Animal, Universidad Austral de Chile, Valdivia, Chile.
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Romero
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile.
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JJ
Núñez
Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile; Centro Fondap Interdisciplinary Center for Aquaculture Research (INCAR), Universidad Austral de Chile, Valdivia, Chile.
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Yáñez
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile. Electronic address: luis.vargas@uach.cl.
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Vargas-Chacoff
D000818:Animals; D001052:Apoferritins / Q000502:physiology*; D005393:Fish Diseases / Q000276:immunology* / Q000378:metabolism; D005399:Fishes / Q000502:physiology*; D007501:Iron / Q000378:metabolism; D053522:Piscirickettsia; D044225:Piscirickettsiaceae Infections / Q000662:veterinary; D059467:Transcriptome
D001052:Apoferritins; D007501:Iron
Eleginops maclovinus; Ferritin; Gene expression; Iron; Notothenioid