Detection and genotyping ofToxoplasma gondiiisolated from soil in Ahvaz, southwest of Iran.
To detection and genotype ofToxoplasma gondiiisolated from soil in Ahvaz, southwest of Iran. Between August 2011 and May 2012 at different sites located in the area of the Ahvaz city south west Iran. A total of 200 soil samples were taken from different points of the region. Oocysts were recovered using the flotation method. Then, PCR reactions targeting the GRA6 gene were performed for specificT. gondiidetection. The positive samples were studied by RFLP (random amplified fragment length polymorphism) usingMseIenzymes to confirm the parasite linage.ToxoplasmaDNA was found in 18 samples. Among them, 12 samples were successfully genotyped as GRA6 type III and 6 as GRA6 Type II. This is the first investigation detecting and genotypingT. gondiioocyst in environmental soil samples of Ahvaz, South west of Iran. The results of this study indicated that soil contaminated withT. gondiioocysts especially in public park may play a role in the epidemiology of human toxoplasmosis in southwest of Iran.
Health Research Institute, Infectious and Tropical Diseases Research Center & Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
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J
Saki
Health Research Institute, Infectious and Tropical Diseases Research Center & Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.|Department of Medical Parasitology and Mycology and Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, PO Box: 613715794, Iran.
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S
Khademvatan
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
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E
Yousefi
Health Research Institute, Infectious and Tropical Diseases Research Center & Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
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M
Tavalla
Health Research Institute, Infectious and Tropical Diseases Research Center & Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
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R
Abdizadeh
Iran; PCR; Soil; Toxoplasma gondii