Antigenotoxic Properties ofAgaricus blazeiagainst Hydrogen Peroxide in Human Peripheral Blood Cells.
The ability ofAgaricus blazeimushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties ofAgaricus blazeion the interventional level, respectively, after treatment.Agaricus blazeiin concentration of 250μg/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2induced DNA damage 15 min after the application ofA. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties ofAgaricus blazeirevealed strong•OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties ofAgaricus blazeiand its strong•OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Lada
0000-0002-8087-1927
L
Živković
Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
Sunčica
S
Borozan
Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Andrea
0000-0002-1182-4895
A
Čabarkapa
Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Dijana
D
Topalović
Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Ummi
U
Ciptasari
Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research "Vinca", University of Belgrade, Belgrade, Serbia.
Vladan
V
Bajić
Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Biljana
0000-0001-5570-883X
B
Spremo-Potparević
D000328:Adult; D000364:Agaricus / Q000737:chemistry* / Q000378:metabolism; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D017665:Hydroxyl Radical / Q000737:chemistry; D007700:Kinetics; D055815:Young Adult
D016587:Antimutagenic Agents; D000975:Antioxidants; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide