Stenotrophomonas sp. RZS 7, a novel PHB degrader isolated from plastic contaminated soil in Shahada, Maharashtra, Western India.
This paper reports an isolation and identification of novel poly-β-hydroxybutyrate (PHB) degrading bacterium Stenotrophomonas sp. RZS 7 and studies on its extracellular PHB degrading depolymerase enzyme. The bacterium isolated from soil samples of plastic contaminated sites of municipal area in Shahada, Maharashtra, Western India. It was identified as Stenotrophomonas sp. RZS 7 based on polyphasic approach. The bacterium grew well in minimal salt medium (MSM) and produced a zone (4.2 mm) of PHB hydrolysis on MSM containing PHB as the only source of nutrient. An optimum yield of enzyme was obtained on the fifth day of incubation at 37 °C and at pH 6.0. Further increase in enzyme production was recorded with Ca2+ions, while other metal ions like Fe2+(1 mM) and chemical viz. mercaptoethanol severally affected the production of enzyme.
Department of Microbiology, PSGVP Mandal's, Arts, Science and Commerce College, Shahada, 425 409, Maharashtra, India.
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SJ
Wani
Department of Microbiology, PSGVP Mandal's, Arts, Science and Commerce College, Shahada, 425 409, Maharashtra, India.
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SS
Shaikh
Department of Zoology, Goverment Raza P G College, Rampur, 244 901, Uttar Pardesh, India.
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B
Tabassum
Microbial Prospection Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box 6, Palampur, 176 061, Himachal Pardesh, India.
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R
Thakur
Microbial Prospection Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box 6, Palampur, 176 061, Himachal Pardesh, India.
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A
Gulati
Department of Microbiology, PSGVP Mandal's, Arts, Science and Commerce College, Shahada, 425 409, Maharashtra, India. sayyedrz@gmail.com.
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RZ
Sayyed
Biodegradation; PHB depolymerase; Poly-β-hydroxybutyrate; Stenotrophomonas sp.