Bactericide, Immunomodulating, and Wound Healing Properties of TransgenicKalanchoe pinnataSynergize with Antimicrobial Peptide Cecropin P1 In Vivo.
Procedure of manufacturingK. pinnatawater extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract ofK. pinnata+ CecP1 exhibits favorable effect on healing of wounds infected withS. aureus(equal to Cefazolin) and with a combination ofS. aureuswithP. aeruginosa(better than Cefazolin). Wild-typeK. pinnataextract exhibited evident microbicide activity againstS. aureuswithP. aeruginosabut it was substantially strengthened inK. pinnata+ CecP1 extract.K. pinnataextracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-typeK. pinnataextract accelerated granulation of the wound bed and marginal epithelialization even better thanK. pinnata+ CecP1 extract. Immunomodulating and microbicide activity ofK. pinnatasynergizes with microbicide activity of CecP1 accelerating elimination of bacteria.
Russian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, Russia.
A A
AA
Lebedeva
Russian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, Russia.
N S
NS
Zakharchenko
Kursk State University, Kursk, Russia; Kursk State Medical University, Kursk, Russia.
E V
EV
Trubnikova
Kursk State Medical University, Kursk, Russia.
O A
OA
Medvedeva
National Institute for Health Development, Tallinn, Estonia.
T V
0000-0002-7285-5038
TV
Kuznetsova
Kazan Federal University, Kazan, Russia.
G A
GA
Masgutova
Russian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, Russia; Emanuel Institute of Biochemical Physics, Moscow, Russia.
M V
MV
Zylkova
Russian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, Russia.
Y I
YI
Buryanov
Kursk State Medical University, Kursk, Russia.
A S
AS
Belous
D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D002437:Cefazolin / Q000627:therapeutic use; D006801:Humans; D056747:Immunomodulation; D031962:Kalanchoe / Q000235:genetics*; D008297:Male; D010455:Peptides / Q000235:genetics / Q000627:therapeutic use*; D010936:Plant Extracts / Q000627:therapeutic use*; D030821:Plants, Genetically Modified; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use*; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000502:physiology*; D013552:Swine; D014945:Wound Healing / Q000187:drug effects; D014946:Wound Infection / Q000188:drug therapy*
D000890:Anti-Infective Agents; D010455:Peptides; D010936:Plant Extracts; D011994:Recombinant Proteins; D002437:Cefazolin; C070016:cecropin P1-LI