Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations.
Conventional photocatalysts are primarily stimulated using ultraviolet (UV) light to elicit reactive oxygen species and have wide applications in environmental and energy fields, including self-cleaning surfaces and sterilization. Because UV illumination is hazardous to humans, visible light-responsive photocatalysts (VLRPs) were discovered and are now applied to increase photocatalysis. However, fundamental questions regarding the ability of VLRPs to trigger DNA mutations and the mutation types it elicits remain elusive. Here, through plasmid transformation and β-galactosidase α-complementation analyses, we observed that visible light-responsive platinum-containing titania (TiO₂) nanoparticle (NP)-mediated photocatalysis considerably reduces the number ofEscherichia colitransformants. This suggests that such photocatalytic reactions cause DNA damage. DNA sequencing results demonstrated that the DNA damage comprises three mutation types, namely nucleotide insertion, deletion and substitution; this is the first study to report the types of mutations occurring after photocatalysis by TiO₂-VLRPs. Our results may facilitate the development and appropriate use of new-generation TiO₂ NPs for biomedical applications.
Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien 97004, Taiwan. dssun@mail.tcu.edu.tw.|Nanobiomedical Research Center, Tzu-Chi University, Hualien 97004, Taiwan. dssun@mail.tcu.edu.tw.
Der-Shan
DS
Sun
Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. tyh@mail.ntust.edu.tw.
Yao-Hsuan
YH
Tseng
Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien 97004, Taiwan. englishbiology@yahoo.com.tw.
Wen-Shiang
WS
Wu
Department of Materials Science and Engineering, National Dong-Hwa University, Hualien 97401, Taiwan. mswong@mail.ndhu.edu.tw.
Ming-Show
MS
Wong
Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien 97004, Taiwan. hhchang@mail.tcu.edu.tw.|Nanobiomedical Research Center, Tzu-Chi University, Hualien 97004, Taiwan. hhchang@mail.tcu.edu.tw.
Hsin-Hou
HH
Chang
DNA mutation; TiO2; lacZ α-complementation; nanoparticle; visible light-responsive photocatalyst