Morphology heterogeneity within a Campylobacter jejuni helical population: the use of calcofluor white to generate rod-shaped C. jejuni 81-176 clones and the genetic determinants responsible for differences in morphology within 11168 strains.
Campylobacter jejuni helical shape is important for colonization and host interactions with straight mutants having altered biological properties. Passage on calcofluor white (CFW) resulted in C. jejuni 81-176 isolates with morphology changes: either a straight morphology from frameshift mutations and single nucleotide polymorphisms in peptidoglycan hydrolase genes pgp1 or pgp2 or a reduction in curvature due a frameshift mutation in cjj81176_1105, a putative peptidoglycan endopeptidase. Shape defects were restored by complementation. Whole genome sequencing of CFW-passaged strains showed no specific changes correlating to CFW exposure. The cjj81176_1279 (recR; recombinational DNA repair) and cjj81176_1449 (unknown function) genes were highly variable in all 81-176 strains sequenced. A frameshift mutation in pgp1 of our laboratory isolate of the straight genome sequenced variant of 11168 (11168-GS) was also identified. The PG muropeptide profile of 11168-GS was identical to that of Δpgp1 in the original minimally passaged 11168 strain (11168-O). Introduction of wild type pgp1 into 11168-GS did not restore helical morphology. The recR gene was also highly variable in 11168 strains. Microbial cell-to-cell heterogeneity is proposed as a mechanism of ensuring bacterial survival in sub-optimal conditions. In certain environments, changes in C. jejuni morphology due to genetic heterogeneity may promote C. jejuni survival.
Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3.
Emilisa
http://orcid.org/0000-0002-9710-9410
E
Frirdich
Institute for Cell and Molecular Biosciences, The Centre for Bacterial Cell Biology, Newcastle University, Newcastle Upon Tyne, NE2 4AX, UK.
Jacob
J
Biboy
Agricultural Research Service, U.S. Department of Agriculture, Produce Safety and Microbiology Research Unit, Albany, CA, 94710, USA.
Steven
S
Huynh
Agricultural Research Service, U.S. Department of Agriculture, Produce Safety and Microbiology Research Unit, Albany, CA, 94710, USA.
Craig T
http://orcid.org/0000-0002-7719-8882
CT
Parker
Institute for Cell and Molecular Biosciences, The Centre for Bacterial Cell Biology, Newcastle University, Newcastle Upon Tyne, NE2 4AX, UK.
Waldemar
W
Vollmer
Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3.
Erin C
EC
Gaynor
D001426:Bacterial Proteins / Q000378:metabolism; D001557:Benzenesulfonates; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000166:cytology* / Q000235:genetics*; D002999:Clone Cells; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D010457:Peptidoglycan / Q000378:metabolism
D001426:Bacterial Proteins; D001557:Benzenesulfonates; D010457:Peptidoglycan; C007061:C.I. Fluorescent Brightening Agent 28