Translation Initiation Factor eIF4E and eIFiso4E Are Both Required forPeanut stripe virusInfection in Peanut (Arachis hypogaeaL.).
Peanut stripe virus(PStV) belongs to the genusPotyvirusand is the most important viral pathogen of cultivated peanut (Arachis hypogaeaL.). The eukaryotic translation initiation factor, eIF4E, and its isoform, eIF(iso)4E, play key roles during virus infection in plants, particularlyPotyvirus. In the present study, we cloned theeIF4EandeIF(iso)4Ehomologs in peanut and named these asPeaeIF4EandPeaeIF(iso)4E, respectively. Quantitative real-time PCR (qRT-PCR) analysis showed that these two genes were expressed during all growth periods and in all peanut organs, but were especially abundant in young leaves and roots. These also had similar expression levels. Yeast two-hybrid analysis showed that PStV multifunctional helper component proteinase (HC-Pro) and viral protein genome-linked (VPg) both interacted with PeaeIF4E and PeaeIF(iso)4E. Bimolecular fluorescence complementation assay showed that there was an interaction between HC-Pro and PeaeIF4E/PeaeIF(iso)4E in the cytoplasm and between VPg and PeaeIF4E/PeaeIF(iso)4E in the nucleus. Silencing eitherPeaeIF4EorPeaeIF(iso)4Eusing a virus-induced gene silencing system did not significantly affect PStV accumulation. However, silencing bothPeaeIF4EandPeaeIF(iso)4Egenes significantly weakened PStV accumulation. The findings of the present study suggest that PeaeIF4E and PeaeIF(iso)4E play important roles in the PStV infection cycle and may potentially contribute to PStV resistance.
Shandong Peanut Research InstituteQingdao, China; Fujian Agriculture and Forestry UniversityFuzhou, China.
Manlin
M
Xu
Shandong Peanut Research Institute Qingdao, China.
Hongfeng
H
Xie
Shandong Peanut Research Institute Qingdao, China.
Juxiang
J
Wu
Fujian Agriculture and Forestry University Fuzhou, China.
Lianhui
L
Xie
Open Project Program of Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences Qingdao, China.
Jinguang
J
Yang
Shandong Peanut Research Institute Qingdao, China.
Yucheng
Y
Chi
Peanut stripe virus; gene silencing; peanut; protein–protein interaction; translation initiation factor 4E