Ultrastructural immunohistochemical study of L-type amino acid transporter 1-4F2 heavy chain in tumor microvasculatures of N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder carcinoma.
Angiogenesis is essential for tumor growth, and an enhanced vasculature supplying nutrients and oxygen might reflect malignant potential. L-type amino acid transporter 1 (LAT1/4F2hc) comprises a major nutrient transport system responsible for the Na+-independent transport of large neutral amino acids. Seventy five to seventy eight percent N-butyl-N-(4-hydroxybutyl) nitrosamine-induced rat bladder carcinoma cells showed high LAT1/4F2hc expression. While the intracarcinoma microvasculatures of fenestrated endothelial cells highly expressing LAT1/4F2hc might progressively transport essential amino acids from the microvasculatures to the extracellular matrix, non-fenestrated endothelial cells and pericytes did not. The present study revealed that the tumor angiogenesis is one of target anti-L-type amino acid transporter 1 drug.
Azabu University School of Veterinary Medicine, Kanagawa, Japan.
Eisuke
E
Kume
Toxicol and Pathol Res, SRICC, Sapporo, Japan.
Tomoko
T
Mutou
Azabu University School of Veterinary Medicine, Kanagawa, Japan.
Norio
N
Kansaku
Department of Pathology, The Jikei University School of Medicine, Tokyo, Japan.
Hitoyuki
H
Takahashi
School of Pharmacy, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Michael F
MF
Wempe
Department of Pathology, The Jikei University School of Medicine, Tokyo, Japan.
Masahiro
M
Ikegami
Department of Pharmacology, Osaka University Graduate School of Medicine, Osaka, Japan, and.
Yoshikatsu
Y
Kanai
J-Pharma Co., Ltd., Tokyo, Japan.
Hitoshi
H
Endou
Azabu University School of Veterinary Medicine, Kanagawa, Japan.|Department of Pathology, The Jikei University School of Medicine, Tokyo, Japan.
Shin
S
Wakui
D000818:Animals; D002085:Butylhydroxybutylnitrosamine / Q000009:adverse effects*; D027282:Fusion Regulatory Protein 1, Heavy Chain / Q000648:ultrastructure*; D007150:Immunohistochemistry / Q000379:methods; D027283:Large Neutral Amino Acid-Transporter 1 / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D055806:Microvessels / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D001749:Urinary Bladder Neoplasms / Q000098:blood supply* / Q000139:chemically induced / Q000648:ultrastructure*
D027282:Fusion Regulatory Protein 1, Heavy Chain; D027283:Large Neutral Amino Acid-Transporter 1; D002085:Butylhydroxybutylnitrosamine
4F2hc; BBN; LAT1; microvasculature of bladder carcinoma; rat; ultrastructural immunohistochemistry