Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.
The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future 'pan-genomic' comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Wen
W
Xie
BGI-Shenzhen, Shenzhen 518083, China.
Chunhai
C
Chen
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Zezhong
Z
Yang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Litao
L
Guo
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Xin
X
Yang
BGI-Shenzhen, Shenzhen 518083, China.
Dan
D
Wang
BGI-Shenzhen, Shenzhen 518083, China.
Ming
M
Chen
BGI-Shenzhen, Shenzhen 518083, China.
Jinqun
J
Huang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Yanan
Y
Wen
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Yang
Y
Zeng
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Yating
Y
Liu
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Jixing
J
Xia
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Lixia
L
Tian
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Hongying
H
Cui
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Qingjun
Q
Wu
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Shaoli
S
Wang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Baoyun
B
Xu
School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.
Xianchun
X
Li
Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Xinqiu
X
Tan
Department of Entomology, Volcani Center, Bet Dagan 5025001, Israel.
Murad
M
Ghanim
Key Lab of Bio-pesticide Creation and Application, South China Agricultural University, Guangzhou 510642, China.
Baoli
B
Qiu
Key Lab of Bio-pesticide Creation and Application, South China Agricultural University, Guangzhou 510642, China.
Huipeng
H
Pan
College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China.
Dong
D
Chu
Cirad, UMR PVBMT, Saint-Pierre, La Réunion, France.
Helene
H
Delatte
Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK.
M N
MN
Maruthi
Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Feng
F
Ge
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Xueping
X
Zhou
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Xiaowei
X
Wang
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Fanghao
F
Wan
School of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China.
Yuzhou
Y
Du
Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China.
Chen
C
Luo
Collaborative Innovation Center of Henan Grain Crops, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Fengming
F
Yan
Department of Biological Sciences, University of Rhode Island, Kingston, Rhode Island 02881, USA.
Evan L
EL
Preisser
College of Life Sciences, Hubei University, Wuhan 430062, China.
Xiaoguo
X
Jiao
United States Department of Agriculture, Agricultural Research Service, Corn Insects & Crop Genetics Research Unit, Ames, IA 50011, USA.
Brad S
BS
Coates
BGI-Shenzhen, Shenzhen 518083, China.
Jinyang
J
Zhao
BGI-Shenzhen, Shenzhen 518083, China.
Qiang
Q
Gao
BGI-Shenzhen, Shenzhen 518083, China.
Jinquan
J
Xia
BGI-Shenzhen, Shenzhen 518083, China.
Ye
Y
Yin
Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Yong
Y
Liu
School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.
Judith K
JK
Brown
Department of Entomology, S-225 Agricultural Science Center North, University of Kentucky, Lexington, KY 40546-0091, USA.
Xuguo Joe
XJ
Zhou
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
Youjun
Y
Zhang
D000818:Animals; D005260:Female; D015723:Gene Library; D049750:Genome, Insect; D006430:Hemiptera / Q000235:genetics*; D008297:Male; D017422:Sequence Analysis, DNA
Annotation; Assembly; Genomics; Whitefly Bemisia tabaci