[Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells].
OBJECTIVE
This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).


METHODS
Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.


RESULTS
Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P<0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) than that in the OIM group.


CONCLUSIONS
Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.
Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China.
Ma
M
Yue
Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China.
Ren
R
Aishu
Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
Fu
F
Gang
D001572:Benzofurans; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D015675:Osteocalcin; D010012:Osteogenesis; D010513:Periodontal Ligament; D053630:Depsides
D001572:Benzofurans; D015675:Osteocalcin; C076944:salvianolic acid B; D053630:Depsides
alkaline phosphatase activity; human periodontal ligament cells; mineralized nodules; osteogenic differentiation; salvianolic acid B