New approach for vertical bone regeneration using in situ gelling and sustained BMP-2 releasing poly(phosphazene) hydrogel system on peri-implant site with critical defect in a canine model.
An injectable hydrogel system with sustained bone morphogenetic protein 2 (BMP-2) release ability was developed for vertical bone regeneration at peri-implant sites and enhanced osseointegration of dental implants. In three young male beagle dogs, a pair of defects was created on both sides of the mandibular bone. Next, two implants were transplanted into each defect. In situ gelling polymer solutions with or without BMP-2 were applied to cover the implants and mandibular defects. The effects of the in situ gelling and sustained BMP-2 releasing (IGSR) hydrogel system on peri-implant bone regeneration were evaluated by radiologic examination, micro-computed tomography, and histomorphometric analysis. Twelve weeks after the treatment, significant bone generation at the peri-implant site occurred following BMP-2/IGSR hydrogel treatment. Bone volume and mineral density were increased by 1.7- and 1.3-fold, respectively (p < 0.01 and 0.05 vs. control, respectively) for the BMP-2/IGSR hydrogel system. And, 0.57-0.31 mm vertical bone generation was observed at the peri-implant site for the BMP-2/IGSR hydrogel system, while rare vertical bone generation occurred in the control group. The BMP-2/IGSR hydrogel system significantly increased bone to implant contact % between induced bone and existing bone (p < 0.05 and 0.01 vs. control). These vertical bone regeneration and higher osseointegration levels demonstrated the effectiveness of the BMP-2/IGSR hydrogel system. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 751-759, 2018.
Center for Biomaterials, Korea Institute of Science & Technology, Seoul, 130-650, Republic of Korea.|Department of Biomolecular Science, University of Science and Technology (UST), Seoul, 136-791, Republic of Korea.
Bo-Bae
BB
Seo
Center for Biomaterials, Korea Institute of Science & Technology, Seoul, 130-650, Republic of Korea.|Research Institute for Basic Sciences and Department of Chemistry, College of Sciences, Kyung Hee University, Seoul, 130-701, Republic of Korea.
Hae-Im
HI
Chang
Department of Pharmacology and Dental Therapeutics, and Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, 500-757, Republic of Korea.
Hyuck
H
Choi
Department of Pharmacology and Dental Therapeutics, and Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, 500-757, Republic of Korea.
Jeong-Tae
JT
Koh
Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, 504-190, Republic of Korea.
Kwi-Dug
KD
Yun
Research Institute for Basic Sciences and Department of Chemistry, College of Sciences, Kyung Hee University, Seoul, 130-701, Republic of Korea.
Jae-Yeol
JY
Lee
Center for Biomaterials, Korea Institute of Science & Technology, Seoul, 130-650, Republic of Korea.|Department of Biomolecular Science, University of Science and Technology (UST), Seoul, 136-791, Republic of Korea.
Soo-Chang
SC
Song
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D004285:Dogs; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008334:Mandible / Q000378:metabolism* / Q000473:pathology; D008338:Mandibular Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology
D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations; D020100:Hydrogels; D009943:Organophosphorus Compounds; D011108:Polymers; C108974:poly(phosphazene)
biodegradable hydrogel; bone morphogenetic protein-2 (BMP-2); in situ forming hydrogel; ossointegration; vertical bone regeneration