Pseudomonas sesami sp. nov., a plant growth-promoting Gammaproteobacteria isolated from the rhizosphere of Sesamum indicum L.
A novel Gram-stain negative, aerobic, motile, rod-shaped bacterium was isolated from Sesame (Sesamum indicum L.) rhizosphere soil. Based on the 16S rRNA gene similarity value (99.4-98.6%) obtained with phylogenetically closely related strains and through analyses of their house keeping genes (atpD, infB and rpoB), the strain SI-P133Twas delineated among the species of the genus Pseudomonas and was subjected to polyphasic taxonomic analysis. It was a chemoorganotroph which grew at wide range of temperature (4-45 °C), pH (5.5-9.5) and NaCl concentrations (0-7% (w/v). DNA-DNA hybridization values with closely related type strains DSM 9751T, DSM 19095T, DSM 21509T, ICMP 9151Tand DSM 6929Tranged from 23.1 to 44.2%. The most abundant fatty acids were C16:0, C10:03-OH, summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), C17:0cyclo and C12:03-OH. The major isoprenoid quinone system was ubiquinone 9 (Q-9) and the G+C content was 61.3 mol%. The major polar lipids of the strain SI-P133Twere diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. With regard to prospective use in agriculture, plant growth-promoting properties of the strain were tested and plant growth-promotion was demonstrated under in vitro conditions. Based on the various polyphasic taxonomic traits analysed, the strain SI-P-133Twas novel and placed within the genus Pseudomonas. Hence we propose a novel species named Pseudomonas sesami sp. nov., for which the type strain is SI-P133T(=NCIMB 14519T= KCTC 22518T).
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore, Tamilnadu, 641 003, India. mmadhaiyan@hotmail.com.|Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore. mmadhaiyan@hotmail.com.
Munusamy
http://orcid.org/0000-0002-0274-8931
M
Madhaiyan
Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore, Tamilnadu, 641 003, India.|Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore.
Selvaraj
S
Poonguzhali
Department of Microbiology, Indira Gandhi College of Arts and Science, Kathirkamam, Pondicherry, 605009, India.
Venkatakrishnan Sivaraj
VS
Saravanan
Department of Microbiology, Indira Gandhi College of Arts and Science, Kathirkamam, Pondicherry, 605009, India.
Kumaran
K
Selvapravin
Addiriyah Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia.
Veeramuthu
V
Duraipandiyan
Addiriyah Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia.
Naif Abdullah
NA
Al-Dhabi
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial; D005227:Fatty Acids; D020563:Gammaproteobacteria / Q000254:growth & development*; D010802:Phylogeny; D011446:Prospective Studies; D011549:Pseudomonas / Q000235:genetics / Q000502:physiology; D012336:RNA, Ribosomal, 16S; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D031685:Sesamum
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
ACC deaminase; P-solubilization; Plant growth-promotion; Pseudomonas sesami; Sesame