Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae.
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.
TU Dresden, Dresden, Germany. Electronic address: roger.dumke@tu-dresden.de.
Roger
R
Dumke
Centers for Disease Control and Prevention, Atlanta, GA, USA.
Alvaro J
AJ
Benitez
Public Health England, London, United Kingdom.
Victoria
V
Chalker
Centre for Research and Development, Uppsala University/Region Gävleborg, Sweden.
Karolina
K
Gullsby
Universität Düsseldorf, Düsseldorf, Germany.
Birgit
B
Henrich
HGC Biomedical Research Diagnostics S.L., Huelva, Spain.
Carlos
C
Hidalgo-Grass
Laboratory of Pediatrics, Department of Pediatrics, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
Theo
T
Hoogenboezem
University of Ljubljana, Ljubljana, Slovenia.
Darja
D
Kese
Universitair Ziekenhuis Antwerpen, Edegem, Belgium.
Katherine
K
Loens
Viroscience, Erasmus MC University Medical Center, Rotterdam, The Netherlands.
Jolanda
J
Maaskant
Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Ayelet
A
Michael-Gayego
Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Allon E
AE
Moses
Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Ran
R
Nir-Paz
Viroscience, Erasmus MC University Medical Center, Rotterdam, The Netherlands.
Suzan D
SD
Pas
University of Bordeaux, Bordeaux, France.
Sabine
S
Pereyre
Statens Serum Institut, Copenhagen, Denmark.
Randi F
RF
Petersen
Universität Düsseldorf, Düsseldorf, Germany.
Meike
M
Rosenblatt
Division of Infectious Diseases and Immunology, Department of Pediatrics, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
Annemarie M C
AMC
van Rossum
Statens Serum Institut, Copenhagen, Denmark.
Søren A
SA
Uldum
Laboratory of Pediatrics, Department of Pediatrics, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
Wendy W J
WWJ
Unger
Universitair Ziekenhuis Antwerpen, Edegem, Belgium.
Dominique
D
Ursi
Centers for Disease Control and Prevention, Atlanta, GA, USA.
Jonas M
JM
Winchell
University of Bordeaux, Bordeaux, France.
Cecile
C
Bebear
D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D006801:Humans; D009177:Mycoplasma pneumoniae / Q000235:genetics* / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial
Detection; Multi-center evaluation; Mycoplasma pneumoniae; Real-time PCR