Residues of Salbutamol and Identification of Its Metabolites in Beef Cattle.
Salbutamol, a selective β2-agonist, endangers the safety of animal products because of its illegal use in food animals. In this work, residues of salbutamol and its metabolites were investigated to select appropriate targets and marker residues for monitoring the illegal use of salbutamol. Ten metabolites of salbutamol were identified from plasma, urine, liver, and kidney samples; of these, six were newly identified. There were significant differences (P < 0.01) between the parent (nonconjugated) and total (conjugated + nonconjugated) salbutamol concentrations in plasma, urine, liver, and kidney tissues. Salbutamol residues in urine were relatively higher than those in plasma and other internal tissues during the dosing period and were rapidly eliminated from plasma, heart, spleen, and kidney tissues during the withdrawal time. Total salbutamol was identified as more preferable than parent salbutamol as a marker residue, and urine and eye tissues were found to be more suitable as targets for preslaughter and postslaughter monitoring of the illegal use of salbutamol in beef cattle.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture , Beijing 100193, China.
Kai
K
Zhang
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture , Beijing 100193, China.
Chaohua
C
Tang
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture , Beijing 100193, China.
Qingshi
Q
Meng
Agilent Technologies (China) Co., Ltd. , Beijing 100102, China.
Wei
W
Du
Agilent Technologies (China) Co., Ltd. , Beijing 100102, China.
Tao
T
Bo
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture , Beijing 100193, China.
Qingyu
Q
Zhao
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture , Beijing 100193, China.
Xiaowei
X
Liang
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture , Beijing 100193, China.
Shengsheng
S
Liu
Agilent Technologies (China) Co., Ltd. , Beijing 100102, China.
Zhixu
Z
Zhang
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture , Beijing 100193, China.
Junmin
http://orcid.org/0000-0002-8405-0536
J
Zhang
D058666:Adrenergic beta-2 Receptor Agonists / Q000097:blood / Q000737:chemistry* / Q000378:metabolism* / Q000652:urine; D000420:Albuterol / Q000097:blood / Q000737:chemistry* / Q000378:metabolism* / Q000652:urine; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000737:chemistry* / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry
D058666:Adrenergic beta-2 Receptor Agonists; D000420:Albuterol
cattle; metabolites; monitoring; residues; salbutamol