Plasma but not serum brain-derived neurotrophic factor concentration is decreased by oral glucose tolerance test-induced hyperglycemia in children.
BACKGROUND
Little is known regarding the relationships among circulating brain-derived neurotrophic factor (BDNF) levels and glucose or insulin in children and adolescents. The objective of this study was to investigate whether circulating BDNF levels would change during the oral glucose tolerance test (OGTT).


METHODS
We performed the OGTT and measured the serial changes in BDNF levels in both plasma and serum.


RESULTS
There were 22 subjects in the normal type (N) group and 20 in the borderline/diabetic type (B/D) group, defined by the results of the OGTT. Serum levels of BDNF were almost five times higher and plasma levels gradually decreased during the OGTT, whereas serum levels showed no significant change. The reduction of plasma BDNF level changes from baseline to 120 min were significantly different between the N and B/D groups (36.3% vs. 20.8%, p=0.023).


CONCLUSIONS
Our results showed that plasma levels of BDNF are more sensitive to acute changes in glucose or insulin levels than serum.
Shunsuke
S
Araki
Yukiyo
Y
Yamamoto
Reiko
R
Saito
Aoi
A
Kawakita
Mami
M
Eguchi
Motohide
M
Goto
Kazuyasu
K
Kubo
Rinko
R
Kawagoe
Yasusada
Y
Kawada
Koichi
K
Kusuhara
D000293:Adolescent; D015415:Biomarkers / Q000097:blood*; D001786:Blood Glucose / Q000032:analysis; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000503:physiopathology*; D005260:Female; D005951:Glucose Tolerance Test / Q000009:adverse effects*; D006801:Humans; D006943:Hyperglycemia / Q000209:etiology*; D007328:Insulin / Q000097:blood; D008297:Male
D015415:Biomarkers; D001786:Blood Glucose; D019208:Brain-Derived Neurotrophic Factor; D007328:Insulin; C579455:BDNF protein, human
brain-derived neurotrophic factor; child and adolescents; glucose tolerance test; insulin; platelets