A review of the current state of antimicrobial susceptibility test methods for Brachyspira.
The re-emergence of swine dysentery (Brachyspira-associated muco-haemorrhagic colitis) since the late 2000s has illuminated diagnostic challenges associated with this genus. The methods used to detect, identify, and characterize Brachyspira from clinical samples have not been standardized, and laboratories frequently rely heavily on in-house techniques. Particularly concerning is the lack of standardized methods for determining and interpreting the antimicrobial susceptibility of Brachyspira spp. The integration of laboratory data into a treatment plan is a critical component of prudent antimicrobial usage. Therefore, the lack of standardized methods is an important limitation to the evidence-based use of antimicrobials. This review will focus on describing the methodological limitations and inconsistencies between current susceptibility testing schemes employed for Brachyspira, provide an overview of what we do know about the susceptibility of these organisms, and suggest future directions to improve and standardize diagnostic strategies.
Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.|Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
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Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.|Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
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D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017400:Brachyspira / Q000187:drug effects*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D008826:Microbial Sensitivity Tests; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D000890:Anti-Infective Agents
Brachyspira; diagnostics vétérinaires; dysenterie porcine; method standardization; standardisation de méthodes; susceptibility test; swine dysentery; test de susceptibilité; veterinary diagnostics