Peroxiredoxin Expression of Human Osteosarcoma Cells Is Influenced by Cold Atmospheric Plasma Treatment.
To evaluate the potential involvement of redox-specific signalling pathways in cold atmospheric plasma (CAP)-induced apoptosis on human osteosarcoma cells.



Osteosarcoma cell lines were treated with CAP with or without antioxidative agents and seeded in cell culture plates. Cell proliferation was determined by counting viable cells. Carrier gas-treated cells served as control. Peroxiredoxin (PRX) 1-3 expression and secretion were assessed.



CAP treatment exhibited strongly attenuated proliferation rates. This effect was significantly attenuated by the addition of N-acetylcysteine (NAC). CAP-treated cells exhibited an increase of PRX 1 and 2 10 sec after treatment. The ratio of oxidized to reduced PRX1 and PRX2 was significantly altered with increasing cellular concentration of the oxidized dimer.



Antioxidant supplementation with NAC increases proliferation of CAP-treated osteosarcoma cells, implicating an involvement of redox signalling. Activation of PRX1 and -2 indicate CAP affects redox homeostasis.
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany denis.guembel@uni-greifswald.de.|Department of Trauma and Orthopaedic Surgery, BG Klinikum Unfallkrankenhaus Berlin gGmbH, Berlin, Germany.
Denis
D
Gümbel
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany.
Nadine
N
Gelbrich
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany.
Matthias
M
Napp
Department of Dermatology, University Medicine Greifswald, Greifswald, Germany.
Georg
G
Daeschlein
Department of Hygiene and Environmental Medicine, University Medicine Greifswald, Greifswald, Germany.
Axel
A
Kramer
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany.
Axel
A
Sckell
Department of Urology, University Medicine Greifswald, Greifswald, Germany.
Martin
M
Burchardt
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany.|Department of Trauma and Orthopaedic Surgery, BG Klinikum Unfallkrankenhaus Berlin gGmbH, Berlin, Germany.
Axel
A
Ekkernkamp
Department of Urology, University Medicine Greifswald, Greifswald, Germany.
Matthias B
MB
Stope
D017209:Apoptosis; D001205:Ascorbic Acid / Q000737:chemistry; D001272:Atmosphere; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006706:Homeostasis; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism*; D010084:Oxidation-Reduction; D054464:Peroxiredoxins / Q000378:metabolism*; D058626:Plasma Gases; D055503:Protein Multimerization; D015398:Signal Transduction
D058626:Plasma Gases; C515940:PRDX1 protein, human; C086065:PRDX2 protein, human; D054464:Peroxiredoxins; D001205:Ascorbic Acid
Osteosarcoma; antitumour efficacy; apoptosis; cold atmospheric plasma; peroxiredoxin