The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of ZoonoticStreptococcus suis.
Like eukaryotes, bacteria express one or more serine/threonine kinases (STKs) that initiate diverse signaling networks. The STK fromStreptococcus suisis encoded by a single-copystkgene, which is crucial in stress response and virulence. To further understand the regulatory mechanism of STK inS. suis, astkdeletion strain (Δstk) and its complementary strain (CΔstk) were constructed to systematically decode STK characteristics by applying whole transcriptome RNA sequencing (RNA-Seq) and phosphoproteomic analysis. Numerous genes were differentially expressed in Δstkcompared with the wild-type parental strain SC-19, including 320 up-regulated and 219 down-regulated genes. Particularly, 32 virulence-associated genes (VAGs) were significantly down-regulated in Δstk. Seven metabolic pathways relevant to bacterial central metabolism and translation are significantly repressed in Δstk. Phosphoproteomic analysis further identified 12 phosphoproteins that exhibit differential phosphorylation in Δstk. These proteins are associated with cell growth and division, glycolysis, and translation. Consistently, phenotypic assays confirmed that the Δstkstrain displayed deficient growth and attenuated pathogenicity. Thus, STK is a central regulator that plays an important role in cell growth and division, as well asS. suismetabolism.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
Chunyan
C
Zhang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
Wen
W
Sun
Veterinary Medicine Laboratory, Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences Nanchang, China.
Meifang
M
Tan
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
Mengmeng
M
Dong
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
Wanquan
W
Liu
Veterinary Medicine Laboratory, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences Wuhan, China.
Ting
T
Gao
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China; Cooperative Innovation Center of Sustainable Pig ProductionWuhan, China.
Lu
L
Li
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China; Cooperative Innovation Center of Sustainable Pig ProductionWuhan, China.
Zhuofei
Z
Xu
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China; Cooperative Innovation Center of Sustainable Pig ProductionWuhan, China.
Rui
R
Zhou
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D015964:Gene Expression Regulation, Bacterial; D053858:Metabolic Networks and Pathways; D051379:Mice; D009154:Mutation; D010766:Phosphorylation; D000072417:Protein Domains; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013290:Streptococcal Infections / Q000382:microbiology*; D017011:Streptococcus suis / Q000502:physiology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism; D015047:Zoonoses
D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors; D017346:Protein Serine-Threonine Kinases
RNA-Seq; Streptococcus suis; eukaryote-like serine/threonine kinase; growth; metabolism; phosphoproteome; phosphorylation; virulence