Novel Acylated Flavonol Tetraglycoside with Inhibitory Effect on Lipid Accumulation in 3T3-L1 Cells from Lu'an GuaPian Tea and Quantification of Flavonoid Glycosides in Six Major Processing Types of Tea.
A novel acylated flavonol tetraglycoside, kaempferol 3-O-[(E)-p-coumaroyl-(1→2)][α-l-arabinopyranosyl-(1→3)][β-d-glucopyranosyl (1→3)-α-l-rhamnopyranosyl(1→6)]-β-d-glucopyranoside (camellikaempferoside C, 1), together with 2 flavonols and 18 flavone and flavonol glycosides (FGs) (2-21) was isolated from the green tea Lu'an GuaPian (Camellia sinensis L.O. Kuntze). Their structures were identified by spectroscopic and chemical methods. Four acylated FGs (1, 7, 8, 9) were found to inhibit the proliferation and differentiation of 3T3-L1 preadipocytes at concentrations of 25, 50, and 100 μM (P < 0.05). Furthermore, we established a rapid UPLC method to quantify nine FGs in six major processing types of tea. The results showed that dark tea had the highest amount of 20 (0.70 ± 0.017 mg/g) and black tea had the highest amount of 8 (0.09 ± 0.012 mg/g), whereas the amounts of 10 and 16 basically decreased with the increasing degree of fermentation and could contribute to the discrimination of different processing types of tea.
Tea Natural Product Laboratory of International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, People's Republic of China.
Wu-Xia
WX
Bai
Tea Natural Product Laboratory of International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, People's Republic of China.
Chao
C
Wang
Tea Natural Product Laboratory of International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, People's Republic of China.
Yi-Jun
YJ
Wang
Tea Natural Product Laboratory of International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, People's Republic of China.
Wen-Jun
WJ
Zheng
Tea Natural Product Laboratory of International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, People's Republic of China.
Wei
W
Wang
Tea Natural Product Laboratory of International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, People's Republic of China.
Xiao-Chun
XC
Wan
Tea Natural Product Laboratory of International Joint Laboratory of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, People's Republic of China.
Guan-Hu
http://orcid.org/0000-0003-4336-5678
GH
Bao
3T3-L1 cells; Camellia sinensis; Lu’an GuaPian; camellikaempferoside C; flavone and flavonol glycosides (FGs)