T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens.
Characterization of allergen-specific T cells is critical to understand their contribution to disease pathogenesis. The identification of immunodominant T-cell epitopes is crucial for development of T-cell-based vaccines. Peptide-specific T-cell proliferation studies are usually performed in a library of short synthetic peptides (15mer or 20mer) with 3 or 5 offset spanning the entire length of the allergen. T-cell peptide epitopes lack the primary and tertiary structure of the native protein to cross-link IgE, but retain the ability to stimulate T cells. The peptides sequences can also be obtained either by in silico approaches and in vitro binding assays. The efficacy of T-cell epitope-based peptide immunotherapy has been proven in certain allergies. The present methodology describes T-cell proliferation assays using whole blood sample from allergic subjects.
Division of Allergy and Immunology, Department of Pediatrics, The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, Icahn Building, Room 11-23B, 1089, New York, NY, 10029, USA. madhan.masilamani@mssm.edu.|Immunology Institute and The Mindich Child Health and Development Institute, Mount Sinai School of Medicine, New York, NY, 10029, USA. madhan.masilamani@mssm.edu.
Madhan
M
Masilamani
Immunology Department, CDB, Hospital Clinic de Barcelona, Universitat de Barcelona, Barcelona, Spain.
Mariona
M
Pascal
Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Hugh A
HA
Sampson
D000485:Allergens / Q000276:immunology*; D049109:Cell Proliferation / Q000502:physiology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007167:Immunotherapy / Q000379:methods; D008213:Lymphocyte Activation / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000485:Allergens; D018984:Epitopes, T-Lymphocyte; D010455:Peptides; D007073:Immunoglobulin E
Epitopes; Peptides; T cell; T cell proliferation; Thymidine incorporation