Antimicrobial resistance, penicillin-binding protein sequences, and pilus islet carriage in relation to clonal evolution of Streptococcus pneumoniae serotype 19A in Russia, 2002-2013.
Clonal changes of serotype 19A pneumococci have been appreciated in conjunction with growing prevalence of this serotype after implementation of the seven-valent pneumococcal conjugate vaccine (PCV7). In the present study, we characterized serotype 19A pneumococci collected in Russia within a decade preceding the implementation of PCV vaccination and described their clonal evolution. We retrospectively analyzed non-invasive serotype 19A isolates collected in 2002-2013. All isolates were subjected to multilocus sequence typing, antimicrobial susceptibility testing, determination of macrolide resistance genotype, molecular detection of pilus islet (PI) carriage, sequencing of penicillin-binding protein (PBP) genes. A total of 49 serotype 19A isolates represented 25 sequence types, of which 14 were newly described. The majority of isolates were distributed among clonal complex (CC) 663 (28%), CC230 (25%), CC156, and CC320 (14% each). CC663 and CC156 dominated in 2003, but were replaced by CC230 and CC320 later on; CC320 was only evident starting 2010. All isolates of CC663 and CC156 carried PI1; CC320 possessed both PI1 and PI2. The overall rate of altered amino acids in penicillin-nonsusceptible isolates was 13·9%, 7·2%, and 8·7% for PBP1a, PBP2b, and PBP2x, respectively. Our findings demonstrate that the clonal structure of serotype 19A pneumococci may evolve without PCV pressure.
Scientific Centre of Children's Health 119991,Lomonosovskiy avenue, 2,Moscow,Russia.
N
N
Mayanskiy
Scientific Centre of Children's Health 119991,Lomonosovskiy avenue, 2,Moscow,Russia.
T
T
Savinova
Scientific Centre of Children's Health 119991,Lomonosovskiy avenue, 2,Moscow,Russia.
N
N
Alyabieva
Scientific Centre of Children's Health 119991,Lomonosovskiy avenue, 2,Moscow,Russia.
O
O
Ponomarenko
Scientific Centre of Children's Health 119991,Lomonosovskiy avenue, 2,Moscow,Russia.
E
E
Brzhozovskaya
Scientific Centre of Children's Health 119991,Lomonosovskiy avenue, 2,Moscow,Russia.
A
A
Lazareva
Scientific Centre of Children's Health 119991,Lomonosovskiy avenue, 2,Moscow,Russia.
L
L
Katosova
Institute of Antimicrobial Chemotherapy, Smolensk State Medical University 214019,46-A Kirova Street,P.O. Box 5,Smolensk,Russia.
R
R
Kozlov
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002675:Child, Preschool; D002999:Clone Cells; D024881:Drug Resistance, Bacterial; D010861:Fimbriae, Bacterial / Q000502:physiology*; D014644:Genetic Variation; D000069443:Heptavalent Pneumococcal Conjugate Vaccine / Q000276:immunology; D006801:Humans; D007223:Infant; D046915:Penicillin-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011008:Pneumococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D015995:Prevalence; D012189:Retrospective Studies; D012426:Russia / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D065288:Serogroup; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000276:immunology / Q000502:physiology*
D000890:Anti-Infective Agents; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D046915:Penicillin-Binding Proteins
Antimicrobial resistance; multilocus sequence typing; penicillin-binding protein; pneumococcus; serotype 19A