A receptor and neuron that activate a circuit limiting sucrose consumption.
The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption inDrosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding inhibition depends on the IR60b receptor, as shown by behavioral analysis and Ca2+imaging of anIR60bmutant. TheIR60bphenotype shows a high degree of chemical specificity when tested with a broad panel of tastants. An automated analysis of feeding behavior in freely moving flies shows that IR60b limits the duration of individual feeding bouts. This receptor and neuron provide the molecular and cellular underpinnings of a new element in the circuit logic of feeding regulation. We propose a dynamic model in which sucrose acts via IR60b to activate a circuit that inhibits feeding and prevents overconsumption.
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
Ryan M
0000-0001-8203-7154
RM
Joseph
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
Jennifer S
0000-0002-4274-0504
JS
Sun
Department of Biomedical Engineering, Yale University, New Haven, United States.
Edric
E
Tam
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
John R
0000-0002-0244-5180
JR
Carlson
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004187:Disaccharides / Q000378:metabolism / Q000494:pharmacology; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000502:physiology; D004435:Eating / Q000502:physiology*; D025461:Feedback, Physiological; D005247:Feeding Behavior / Q000502:physiology*; D015870:Gene Expression; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D009005:Monosaccharides / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061848:Optical Imaging; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology*; D013649:Taste / Q000502:physiology; D055697:Taste Perception / Q000502:physiology
D004187:Disaccharides; D029721:Drosophila Proteins; D009005:Monosaccharides; D043562:Receptors, G-Protein-Coupled; D013395:Sucrose; D005990:Glycerol; D002118:Calcium
D. melanogaster; chromosomes; dietary regulation; feeding inhibition; genes; gustatory receptor; ionotropic receptor; neuroscience; pharynx; taste