Ultrasound-assisted ionic liquid-based micellar extraction combined with microcrystalline cellulose as sorbent in dispersive microextraction for the determination of phenolic compounds in propolis.
An ionic liquid-(IL) based micellar extraction combined with microcrystalline cellulose- (MCC) assisted dispersive micro solid-phase extraction method was developed to extract phenolic compounds from propolis. A total of 20 target compounds were identified by ultra-high- performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. The main extraction parameters were optimized and included the ultrasonic power, ultrasonic time, sample pH, type of IL, the concentration of [C12mim]Br, extraction time, concentration of MCC, type of sorbent and type of elution solvents. Under the optimum conditions, the proposed method exhibited good linearities (r2≥ 0.999) for all plant phenolic compounds with the lower limits of detection in the range of 0.21-0.41 ng/mL. The recoveries ranged from 82.74% to 97.88% for pinocembrin, chrysin and galangin. Compared with conventional solvent extraction, the present method was simpler and more efficient and required less organic solvent and a shorter extraction time. Finally, the methodology was successfully used for the extraction and enrichment of phenolic compounds in propolis.
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
Jun
J
Cao
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Li-Qing
LQ
Peng
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Li-Jing
LJ
Du
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Qi-Dong
QD
Zhang
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Jing-Jing
JJ
Xu
D000327:Adsorption; D002482:Cellulose / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D059627:Liquid Phase Microextraction / Q000379:methods*; D008823:Micelles; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D011429:Propolis / Q000737:chemistry*; D000069453:Ultrasonic Waves
D052578:Ionic Liquids; D008823:Micelles; D010636:Phenols; D002482:Cellulose; D011429:Propolis; C109691:microcrystalline cellulose
Dispersive micro solid-phase extraction; Ionic liquid; Microcrystalline cellulose; Propolis; Quadrupole time-of-flight tandem mass spectrometry; Ultra-high- performance liquid chromatography