Synthesis of 1,3-distearoyl-2-oleoylglycerol by enzymatic acidolysis in a solvent-free system.
1,3-Distearoyl-2-oleoylglycerol (SOS) is widely used as a cocoa butter improver and an anti-blooming agent in the chocolate industry. In this study, an effective process was developed to prepare SOS by enzymatic acidolysis. Under optimal reaction conditions (substrate molar ratio of 12, NS40086 loading of 10%, a solvent-free system, 75°C for 4h), a SOS yield of 70.2% was obtained. Subsequently, two-step purification was applied to purify the crude SOS product. Free fatty acids were completely removed after molecular distillation. After acetone fractionation, the purity of SOS reached 92.2% with an 85.1% recovery. Comparison of various physicochemical properties of purified SOS and a commercial cocoa butter improver showed their properties to be similar. This study provides an effective and sustainable process for the synthesis of SOS product, which is expected to be used as a high-quality cocoa butter improver or an anti-blooming agent in the chocolate industry.
State Key Laboratory of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China. Electronic address: wxstongxue@163.com.
Xiaosan
X
Wang
State Key Laboratory of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Yang
Y
Chen
State Key Laboratory of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Liyou
L
Zheng
State Key Laboratory of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Qingzhe
Q
Jin
State Key Laboratory of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Xingguo
X
Wang
D004041:Dietary Fats; D010938:Plant Oils / Q000737:chemistry*; D014280:Triglycerides / Q000138:chemical synthesis* / Q000737:chemistry*
C117423:1,3-distearoyl-2-oleoylglycerol; D004041:Dietary Fats; D010938:Plant Oils; D014280:Triglycerides; C052387:cocoa butter
1,3-Distearoyl-2-oleoylglycerol; Acidolysis; Anti-blooming agent; Fractionation; NS40086 lipase