Validated RP-HPLC/DAD Method for the Quantification of Insect Repellent Ethyl 2-Aminobenzoate in Membrane-Moderated Matrix Type Monolithic Polymeric Device.
A simple, accurate and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the estimation of ethyl 2-aminobenzoate (EAB) in a matrix type monolithic polymeric device and validated as per the International Conference on Harmonization guidelines. The analysis was performed isocratically on a ZORBAX Eclipse plus C18 analytical column (250 × 4.4 mm, 5 μm) and a diode array detector (DAD) using acetonitrile and water (75:25 v/v) as the mobile phase by keeping the flow-rate constant at 1.0 mL/min. Determination of EAB was not interfered in the presence of excipients. Inter- and intra-day relative standard deviations were not higher than 2%. Mean recovery was between 98.7 and 101.3%. Calibration curve was linear in the concentration range of 0.5-10 µg/mL. Limits of detection and quantification were 0.19 and 0.60 µg/mL, respectively. Thus, the present report put forward a novel method for the estimation of EAB, an emerging insect repellent, by using RP-HPLC technique.
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.|Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam 786004, India.
Johirul
J
Islam
Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam 786004, India.
Kamaruz
K
Zaman
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.
Srijita
S
Chakrabarti
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.
Nilutpal
N
Sharma Bora
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.
Santa
S
Mandal
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.
Manash
M
Pratim Pathak
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.
Pakalapati
P
Srinivas Raju
Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam 784001, India.
Pronobesh
P
Chattopadhyay
D001566:Benzocaine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D007302:Insect Repellents / Q000032:analysis*; D016014:Linear Models; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007302:Insect Repellents; D008567:Membranes, Artificial; D011108:Polymers; D001566:Benzocaine