The long non-coding RNA LINC01013 enhances invasion of human anaplastic large-cell lymphoma.
Anaplastic large-cell lymphoma (ALCL) is a rare type of highly malignant, non-Hodgkin lymphoma (NHL). Currently, only studies on the chimeric oncogene NPM-ALK have reported a link to ALCL progression. However, the specific molecular mechanisms underlying the invasion of ALCL are still unclear. Here, we sought to investigate differentially expressed, long non-coding RNAs (lncRNAs) in ALCL and their potential biological function. Our microarray analyses revealed that LINC01013, a novel non-coding RNA gene, was highly expressed in clinical specimens of ALCL and was significantly upregulated in invasive ALCL cell lines. Knockdown of LINC01013 suppressed tumor cell invasion; conversely, its overexpression enhanced tumor cell invasion. LINC01013-induced invasion was mediated by activation of the epithelial-to-mesenchymal transition (EMT)-associated proteins, snail and fibronectin. Specifically, LINC01013 induced snail, resulting in activation of fibronectin and enhanced ALCL cell invasion. Collectively, these findings support a potential role for LINC01013 in cancer cell invasion through the snail-fibronectin activation cascade and suggest that LINC01013 could potentially be utilized as a metastasis marker in ALCL.
Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan (R.O.C.).
I-Hsiao
IH
Chung
Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan (R.O.C.).|Department of Dermatology, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan (R.O.C.).
Pei-Hsuan
PH
Lu
Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan (R.O.C.).
Yang-Hsiang
YH
Lin
Department of Nursing, Chang Gung University of Science and Technology, Taoyuan, Taiwan (R.O.C.).|Department of General Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan (R.O.C.).
Ming-Ming
MM
Tsai
Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan (R.O.C.).
Yun-Wen
YW
Lin
Liver Research Center, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan (R.O.C.).
Chau-Ting
CT
Yeh
Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan (R.O.C.). khlin@mail.cgu.edu.tw.|Liver Research Center, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan (R.O.C.). khlin@mail.cgu.edu.tw.|Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan (R.O.C.). khlin@mail.cgu.edu.tw.
Kwang-Huei
KH
Lin
D058750:Epithelial-Mesenchymal Transition; D005353:Fibronectins / Q000378:metabolism; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D017728:Lymphoma, Large-Cell, Anaplastic / Q000473:pathology*; D046228:Microarray Analysis; D009361:Neoplasm Invasiveness; D062085:RNA, Long Noncoding / Q000378:metabolism*; D000071250:Snail Family Transcription Factors / Q000378:metabolism
D005353:Fibronectins; D062085:RNA, Long Noncoding; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors