Nanozyme-based bio-barcode assay for high sensitive and logic-controlled specific detection of multiple DNAs.
Since HCV and HIV share a common transmission path, high sensitive detection of HIV and HCV gene is of significant importance to improve diagnosis accuracy and cure rate at early stage for HIV virus-infected patients. In our investigation, a novel nanozyme-based bio-barcode fluorescence amplified assay is successfully developed for simultaneous detection of HIV and HCV DNAs with excellent sensitivity in an enzyme-free and label-free condition. Here, bimetallic nanoparticles, PtAuNPs, present outstanding peroxidase-like activity and act as barcode to catalyze oxidation of nonfluorescent substrate of amplex red (AR) into fluorescent resorufin generating stable and sensitive "Turn On" fluorescent output signal, which is for the first time to be integrated with bio-barcode strategy for fluorescence detection DNA. Furthermore, the provided strategy presents excellent specificity and can distinguish single-base mismatched mutant from target DNA. What interesting is that cascaded INHIBIT-OR logic gate is integrated with biosensors for the first time to distinguish individual target DNA from each other under logic function control, which presents great application in development of rapid and intelligent detection.
Key laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Xiaodong
X
Lin
Key laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: yaqingliu@tust.edu.cn.
Yaqing
Y
Liu
Key laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Zhanhui
Z
Tao
Key laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Jinting
J
Gao
Key laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Jiankang
J
Deng
Key laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Jinjin
J
Yin
Key laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: s.wang@tust.edu.cn.
Shuo
S
Wang
D015374:Biosensing Techniques; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification*; D006678:HIV / Q000737:chemistry / Q000302:isolation & purification*; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology; D016174:Hepacivirus / Q000737:chemistry / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis / Q000821:virology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D004279:DNA, Viral
Bio-barcode assay; Biosensors; Molecular logic gate; Multiple DNA detection; Nanozyme