LC-ESI-QTOF-MS identification of novel antioxidant peptides obtained by enzymatic and microbial hydrolysis of vegetable proteins.
Bioactive antioxidant peptides are more and more attracting the attention of food manufacturers for their potential to transform food in functional food, able to prevent a variety of chronic diseases associated with oxidative stress. In the present study proteins extracted from different vegetable sources (KAMUT® khorasan wheat, emmer, lupine and pea) were hydrolyzed with commercial enzymes and Lactobacillus spp. strains. Hydrolysates were separated by size exclusion chromatography and purified fractions were analyzed for their antioxidant activity. Peptides from the fractions with the highest activity were identified by nanoLC-ESI-QTOF-MS and thirteen peptides were selected for synthesis on the basis of their sequence. Four peptides (VLPPQQQY, TVTSLDLPVLRW, VTSLDLPVLRW, FVPY) were found able to scavenge superoxide anion and hydroxyl radicals, organic nitro-radicals (ABTS, DPPH) and to inhibit lipid peroxidation. The impact of this work is targeted to add hydrolysed vegetable proteins to reformulated functional food or to produce health-promoting ingredients and nutraceuticals.
Department of Agri-Food Sciences and Technologies, Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy. Electronic address: elena.babini2@unibo.it.
Elena
E
Babini
Department of Life Sciences, University of Modena and Reggio Emilia, Via Amendola 2, 42122 Reggio Emilia, Italy. Electronic address: davide.tagliazucchi@unimore.it.
Davide
D
Tagliazucchi
Department of Life Sciences, University of Modena and Reggio Emilia, Via Amendola 2, 42122 Reggio Emilia, Italy. Electronic address: serena.martini@unimore.it.
Serena
S
Martini
Department of Agri-Food Sciences and Technologies, Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy. Electronic address: lucilla2.deipiu@gmail.com.
Lucilla
L
Dei Più
Department of Agri-Food Sciences and Technologies, Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy. Electronic address: andrea.gianotti@unibo.it.
Andrea
A
Gianotti
D000975:Antioxidants / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D006868:Hydrolysis; D015227:Lipid Peroxidation; D013058:Mass Spectrometry / Q000379:methods*; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D014674:Plant Proteins, Dietary / Q000737:chemistry*
D000975:Antioxidants; D010455:Peptides; D014674:Plant Proteins, Dietary
Antioxidant peptides; Enzymatic hydrolysis; Lactobacillus; Microbial hydrolysis; Nano LC-ESI-QTOF-MS; Vegetable proteins