Melaxillines A and B, monoterpenoid indole alkaloids from Melodinus axillaris.
Two new monoterpenoid indole alkaloids, melaxillines A (1) and B (2), together with four known alkaloids (3-6), were isolated from the roots of Melodinus axillaris. The structures were determined by extensive spectroscopic methods, including 1D and 2D-NMR spectroscopy, high resolution mass spectrometric data, and by comparison to literature data. New compounds 1-2 might be derived from a common precursor (3) by proposing their biogenetic route, and compound 1 represents the first Melodinus alkaloid featuring an ethyl acetate group skeleton at C-18. The anti-inflammatory effects of all the isolated compounds were evaluated by measuring the inhibitory ratios of β-glucuronidase release in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor. Compounds 1-4 showed significant anti-inflammatory activities with IC50values ranging from 1.51 to 21.56μM, respectively.
College of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China; Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China.
Lei
L
Fang
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China.
Shan-Ming
SM
Tian
College of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China; Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China. Electronic address: zhoujie8761@163.com.
Jie
J
Zhou
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China.
Yun-Liang
YL
Lin
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China.
Zhi-Wei
ZW
Wang
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China. Electronic address: wxjn1998@126.com.
Xiao
X
Wang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification; D029062:Apocynaceae / Q000737:chemistry*; D002478:Cells, Cultured; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D007962:Leukocytes / Q000187:drug effects; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry* / Q000302:isolation & purification
D000893:Anti-Inflammatory Agents; D046948:Secologanin Tryptamine Alkaloids; C000616671:melaxilline A; C000616672:melaxilline B; D005966:Glucuronidase
Anti-inflammatory; Apocynaceae; Melodinus axillaris; Monoterpenoid indole alkaloid