Phycospheric Native BacteriaPelagibaca bermudensisandStappiasp. Ameliorate Biomass Productivity ofTetraselmis striata(KCTC1432BP) in Co-cultivation System through Mutualistic Interaction.
Effective sustainable algal cultivation techniques are essential for mass production of the marine microalgaTetraselmisfor biofuel and array of co-products. The phycospheric communities affect the microalgal growth and metabolism through various allelochemical and nutrient interactions; hence, their potential to affect the quantity and quality of both biomass and bioproducts is significant. In the present study, we have screened the phycospheric communities of biofuel producingTetraselmis striata(KCTC1432BP). A total of 26 bacterial strains were isolated and identified from the phycosphere ofT. striatamass culture. Then, each bacterial strain was tested in co-cultivation conditions withT. striatafor evaluating its growth promoting and inhibitory effects. Among these all strains, two promising strains (Pelagibaca bermudensisKCTC 13073BP andStappiasp. KCTC 13072BP) were selected because of their maximum growth promoting effects and mutualistic interactions. The growth rate, biomass productivity, lipid contents, and fatty acids were analyzed during their combined growth in O3 media and compared with axenic growth ofT. striata. Later, growth promoting mechanisms in the co-cultivation environment were investigated for these promising bacterial strains under replete and limited conditions of nutrients (nitrate, phosphate, and vitamin B12). The growth promoting potential ofP. bermudensiswas illustrated by the two fold enhancement in biomass productivity. These bacteria are promising for microalgal cultivation without any negative effects on the native seawater bacterial communities, as revealed by next generation sequencing analysis. This study represents, to date, the first report highlighting the role of phycospheric growth promoting bacteria of promising biofuel feedstockT. striata.
Department of Life Science, College of Natural Sciences and Research Institute for Natural Sciences, Hanyang University Seoul, South Korea.
Jungsoo
J
Park
Department of Life Science, College of Natural Sciences and Research Institute for Natural Sciences, Hanyang University Seoul, South Korea.
Bum Soo
BS
Park
Department of Life Science, College of Natural Sciences and Research Institute for Natural Sciences, Hanyang University Seoul, South Korea.
Pengbin
P
Wang
Department of Life Science, College of Natural Sciences and Research Institute for Natural Sciences, Hanyang University Seoul, South Korea.
Shailesh K
SK
Patidar
Department of Life Science, College of Natural Sciences and Research Institute for Natural Sciences, Hanyang University Seoul, South Korea.
Jin Ho
JH
Kim
Department of Life Science, College of Natural Sciences and Research Institute for Natural Sciences, Hanyang University Seoul, South Korea.
Sae-Hee
SH
Kim
Department of Life Science, College of Natural Sciences and Research Institute for Natural Sciences, Hanyang University Seoul, South Korea.
Myung-Soo
MS
Han
Tetraselmis; biofuel; biomass; growth promoting bacteria; lipid; phosphate; phycosphere