Multi-spectroscopic and molecular modeling studies of bovine serum albumin interaction with sodium acetate food additive.
Sodium acetate (SA) has been used as a highly effective protectant in food industry and the possible effect of this additive on the binding to albumin should be taken into consideration. Therefore, for the first time, the mechanism of SA interaction with bovine serum albumin (BSA) has been investigated by multi-spectroscopic and molecular modeling methods under physiological conditions. Stern-Volmer fluorescence quenching analysis showed an increase in the fluorescence intensity of BSA upon increasing the amounts of SA. The high affinity of SA to BSA was demonstrated by a binding constant value (1.09×103at 310°K). The thermodynamic parameters indicated that hydrophobic binding plays a main role in the binding of SA to Albumin. Furthermore, the results of UV-vis spectra confirmed the interaction of this additive to BSA. In addition, molecular modeling study demonstrated that A binding sites of BSA play the main role in the interaction with acetate.
Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Hossein
H
Mohammadzadeh-Aghdash
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: ezzatij@tbzmed.ac.ir.
Jafar
J
Ezzati Nazhad Dolatabadi
Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: dehghan.nut@gmail.com.
Parvin
P
Dehghan
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Vahid
V
Panahi-Azar
Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran.
Abolfazl
A
Barzegar
D000818:Animals; D002417:Cattle; D005503:Food Additives / Q000737:chemistry*; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019346:Sodium Acetate / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005503:Food Additives; D012710:Serum Albumin, Bovine; D019346:Sodium Acetate
Bovine serum albumin; Fluorescence quenching; Molecular docking; Sodium acetate