Effect of Pelargonidin isolated fromFicus benghalensisL. on phenotypic changes in zebrafish (Danio rerio) embryos.
In the present study, the extraction and isolation of Pelargonidin, an anthocyanin compound from stem bark ofFicus benghalensisare described.The study also involves evaluation of the effect of Pelargonidin on phenotypic variations in zebra fish embryos. Extraction and isolation of Pelargonidin were carried out by employing liquid-liquid extraction technique, phytochemical tests, column chromatography, UV and FT-IR. In the zebra fish embryo model, Paclitaxel was employed as a negative control. A series of phenotypic changes in different stages of embryonic development were studied with treatment concentrations of Pelargonidin between 3.0 and 20 ppm at 0-72-hour post-fertilization (hpf). The results of our studies indicate that, after exposure of zebra fish embryos to 3.3-20 ppm concentration of Pelargonidin for 72 h, a significant reduction in aortic development occurs. At the dose level of 0.5 ppm Paclitaxel and Pelargonidin in the dose range between 3.3 and 20 ppm, the zebra fish embryos were found to have bent tail, malformed eyes and developmental delays in vasculature. Based on the results obtained, we infer that Pelargonidin can exhibit phenotypic anti-angiogenic variations in embryonic stage of fish embryos and it can be applied in future for exploration of its anti-angiogenic potential. Furthermore, Pelargonidin could serve as a candidate drug forin vivoinhibition of angiogenesis and can be applied for the treatment of neovascular diseases and tumor.
Neuropharmacology Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor Darul Ehsan, Malaysia.
Uday
U
Kundap
Center for Excellence in Post-Harvest Technologies, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA.
Yogini
Y
Jaiswal
CHM Campus, Chandibai Himmathmal Mansukhani Road, Opp. Ulhasnagar Railway Station, Ulhasnagar, Maharashtra 421003, India.
Rachana
R
Sarawade
Center for Excellence in Post-Harvest Technologies, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA.
Leonard
L
Williams
Neuropharmacology Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor Darul Ehsan, Malaysia.
Mohd Farooq
MF
Shaikh
Angiogenesis; Anthocyanin; Pelargonidin; Phenotypic variation; Zebra fish embryos