Establishment of Reference Interval for Alkaline Phosphatase in Healthy Children of Various Ethnicities, Aged 0-12 Years.
BACKGROUND
To establish a reference interval of alkaline phosphatase for healthy children of various ethnicities, aged 0 years to 12 years in China.


METHODS
According to the Clinical and Laboratory Standards Institute guideline C28-A3, blood specimens were collected in the morning after overnight fasting from 502 healthy children to determine the alkaline phosphatase (ALP) level, using the Beckman AU5800 automatic biochemical analyzer. Children were grouped by sex and age. The age groups were as follows: 0 to 1 year, 1 to 3 years, 3 to 6 years, and 6 to 12 years.


RESULTS
There were no significant differences between the sex and age groups for the age groups of 0 to 1 year, 1 to 3 years, and 3 to 6 years; as a result, we combined those groups into a single group. The reference interval of ALP is 81.9 to 350.3 U per L in the group aged 0 to 6 years and 48.8 to 445.9 U per L in the group aged 6 to 12 years.


CONCLUSIONS
The results of this study establish a reference interval for ALP in healthy children, which will provide a basis for screening, diagnosing, and monitoring bone or liver-related diseases in children.
Department of Clinical Laboratory, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University School of Medicine, Nanjing University, Nanjing, China.|Department of Clinical Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
Gu
G
Wanjian
Department of Clinical Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
Hu
H
Jie
Department of Clinical Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
Ge
G
Liang
Department of Clinical Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
Wang
W
Cheng
Department of Clinical Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
Xu
X
Tian
Department of Clinical Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
Shen
S
Jianjiang
Department of Clinical Laboratory, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University School of Medicine, Nanjing University, Nanjing, China.
Zhang
Z
Chunni
D000469:Alkaline Phosphatase / Q000097:blood*; D001774:Blood Chemical Analysis / Q000592:standards*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008111:Liver Function Tests; D008297:Male; D012016:Reference Values
D000469:Alkaline Phosphatase
alkaline phosphatase; children; liver function; performance verification; reference interval; skeletal disease