Yolk-shell nanostructured Fe3O4@C magnetic nanoparticles with enhanced peroxidase-like activity for label-free colorimetric detection of H2O2and glucose.
Herein, we have developed a simple and facile method to synthesize yolk-shell nanostructured Fe3O4@C nanoparticles (NPs) as a multifunctional biosensing platform for the label-free colorimetric detection of H2O2and glucose. It was demonstrated that Fe3O4@C yolk-shell nanostructures (YSNs) retained the magnetic properties that can be used for separation and concentration. Also importantly, the Fe3O4@C YSNs exhibited an intrinsic peroxidase-like activity that could quickly catalyze the enzyme substrate in the presence of H2O2and produce a blue color. Compared to other similar ferric oxide-based NPs with different structures, Fe3O4@C YSNs exhibited greatly enhanced catalytic activities due to their unique structural features. Moreover, steady-state kinetics indicated the catalytic behaviors in agreement with the classic Michaelis-Menten models. Taking advantage of the high catalytic activity, Fe3O4@C YSNs were employed as novel peroxidase mimetics for label-free, rapid, sensitive, and specific colorimetric sensing of H2O2and glucose, suggesting that Fe3O4@C YSNs have the potential for construction of portable sensors in the application of point-of-care (POC) diagnosis and on-site tests.
School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
Na
http://orcid.org/0000-0001-5771-247X
N
Lu
College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. zhangmin@sues.edu.cn.
Min
M
Zhang
College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. zhangmin@sues.edu.cn.
Lei
L
Ding
College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. zhangmin@sues.edu.cn.
Jing
J
Zheng
School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
Caixia
C
Zeng
Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China.
Yanli
Y
Wen
Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China.
Gang
G
Liu
Chemistry Department, King Saud University, Riyadh 11451, Saudi Arabia.
Ali
A
Aldalbahi
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. wanglihua@sinap.ac.cn.
Jiye
J
Shi
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. wanglihua@sinap.ac.cn.
Shiping
S
Song
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. wanglihua@sinap.ac.cn.
Xiaolei
X
Zuo
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. wanglihua@sinap.ac.cn.
Lihua
L
Wang
D003124:Colorimetry; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000032:analysis*; D058185:Magnetite Nanoparticles; D009195:Peroxidase / Q000737:chemistry*
D058185:Magnetite Nanoparticles; D006861:Hydrogen Peroxide; D009195:Peroxidase; D005947:Glucose