Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis.
BACKGROUND
Lysine-specific histone demethylase 1 (LSD1) modulates chromatin status through demethylation of H3K4 and H3K9. It has been demonstrated that LSD1 is hyperphosphorylated and dissociates from chromatin during mitosis. However, the molecular mechanism of LSD1 detachment is unknown.


RESULTS
In this report, we found that polo-like kinase 1 (PLK1) directly interacted with LSD1 and phosphorylated LSD1 at Ser-126 . Nocodazole-induced metaphase arrest promoted release of LSD1 from chromatin, and the phosphorylation-defective mutant LSD1 (S126A) failed to dissociate from chromatin upon nocodazole treatment.


CONCLUSIONS
Taken together, our findings demonstrate that phosphorylation of LSD1 at Ser-126 by PLK1 promotes its release from chromatin during mitosis.
Beijing Key Laboratory of DNA Damage Response and College of Life Sciences, Capital Normal University, Beijing, 100048 China.|Guangdong Key Laboratory of Genome Stability & Disease Prevention, Shenzhen University School of Medicine, Shenzhen, 518060 Guangdong China.
Bin
B
Peng
Beijing Key Laboratory of DNA Damage Response and College of Life Sciences, Capital Normal University, Beijing, 100048 China.
Ruifeng
R
Shi
Beijing Key Laboratory of DNA Damage Response and College of Life Sciences, Capital Normal University, Beijing, 100048 China.
Weiwei
W
Jiang
National Institute of Biological Sciences, Beijing, 102206 China.
Yue-He
YH
Ding
National Institute of Biological Sciences, Beijing, 102206 China.
Meng-Qiu
MQ
Dong
Guangdong Key Laboratory of Genome Stability & Disease Prevention, Shenzhen University School of Medicine, Shenzhen, 518060 Guangdong China.
Wei-Guo
WG
Zhu
Beijing Key Laboratory of DNA Damage Response and College of Life Sciences, Capital Normal University, Beijing, 100048 China.|Guangdong Key Laboratory of Genome Stability & Disease Prevention, Shenzhen University School of Medicine, Shenzhen, 518060 Guangdong China.
Xingzhi
X
Xu
Chromatin release; Lysine-specific demethylase LSD1; Mitosis; PLK1; Phosphorylation