Molecular characterization ofLeishmaniaparasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran.
Cutaneous leishmaniasis (CL) is vector borne parasitic disease, considered as public health problem especially in border of Iran and Iraq, Dehloran County (Musian district). The aim of this study was molecular identification ofLeishmaniaparasites in sandfly as vectors of Leishmaniasis. Totally 280 female sandflies were trapped by sticky traps from 7 rural areas of Musiyan in September-November 2012. All sandflies were identified using morphological characters of the head and abdominal terminalia. DNA was extracted from female sandflies andLeishmaniawas identified using PCR and sequencing. All 280 trapped sandflies were identified asPhelobotumus PapatasiandLeishmaniainfections were detected in 3.2 % out of 280 female sandflies. All leishmania were identified asL. majorand submitted in Gene bank as: LC014642.1, LC014641.1, LC014640.1 and LC014639.1. Frequency ofPhlebotomus Papatasiand infection withL. majorin studied regions showed that this vector is dominant in these areas.
Department of Medical Parasitology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Farzaneh
F
Kavarizadeh
Department of Medical Parasitology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.|Department of Medical Parasitology and Mycology and Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Shahram
S
Khademvatan
Department of Medical Entomology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Babak
B
Vazirianzadeh
Department of Medical Parasitology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mohammad Hossein
MH
Feizhaddad
Department of Medical Parasitology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mehdi
M
Zarean
Characterization; Iran; L. major; Sandfly