Antimicrobial photodynamic therapy combined to periodontal treatment: Experimental model.
BACKGROUND
Antimicrobial photodynamic therapy (aPDT) has been used as an adjuvant treatment for periodontitis. It combines a photosensitizer with a light source to induce reactive oxygen species and kill microbial cells. PpNetNI is a protoporphyrin derivative, and it has a chemical binding site at biofilm and great affinity to microbial cells. The aim of this study was to investigate the effects of aPDT as an adjuvant treatment for periodontitis.


METHODS
Thirty healthy male rats Wistar (Rattus norvegicus) were used in this study (Approved by UNINOVE Ethical committee AN0029/2015). Periodontitis was induced by placing a cotton ligature around the first mandibular molar in a subgengival position. The contralateral mandibular first molar received neither a ligature nor any treatment, and was used as a control. After 7 days, the ligature was removed and all animals received scaling and root planing (SRP) and were divided according to the following treatments: SRP group (received SRP and irrigation with PpNetNI, 10μM) and aPDT group (PpNetNI 10μM followed by LED irradiation). aPDT was performed with a LED (630nm) with an output power of 400mW (fluence-rate 200mW/cm2; fluence 18J/cm2). Rats were euthanized at 24h, 48h and 7days postoperatively. The area of bone loss in vestibular region of the first molar was evaluated by Optical Coherence Tomography (OCT, THORLABS LTD., Ely, UK). Data were analyzed statistically (ANOVA and Tukey tests, p<0.05).


RESULTS
The animals treated by aPDT showed bone gain of approximately 30% compared to the SRP group following 7days from the treatment.


CONCLUSIONS
aPDT promoted bone recovery 7days after periodontal intervention.
Dep. of Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Estéfani L
EL
Belinello-Souza
Dep. of Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil; School of Dentistry, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil. Electronic address: leticiahalvarenga@uni9.pro.br.
Letícia H
LH
Alvarenga
Dep. of Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil; School of Dentistry, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Cintia
C
Lima-Leal
Dep. of Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Patrícia
P
Almeida
School of Dentistry, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Carolina Guimarães
CG
Leite
School of Dentistry, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Tairine R
TR
Lima
Dep. of Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil; School of Dentistry, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Bianca
B
Godoy-Miranda
School of Dentistry, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Jhosepher
J
Previati-Oliveira
Center for Lasers and Applications - IPEN-CNEN/SP, São Paulo, Brazil.
Lucas
L
de Pretto
Center for Lasers and Applications - IPEN-CNEN/SP, São Paulo, Brazil.
Anderson Zanardi
AZ
de Freitas
Universidade Anhembi Morumbi, São Paulo, Brazil.
Adjaci U
AU
Fernandes
Dep. of Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Rodrigo
R
Labat Marcos
Dep. of Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil; School of Dentistry, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil. Electronic address: pratesra@uni9.pro.br.
Renato A
RA
Prates
D000818:Animals; D003131:Combined Modality Therapy / Q000379:methods; D012534:Dental Scaling / Q000379:methods*; D004195:Disease Models, Animal; D004203:Disinfection / Q000379:methods*; D008297:Male; D010518:Periodontitis / Q000188:drug therapy* / Q000473:pathology / Q000628:therapy*; D010778:Photochemotherapy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
Antimicrobial photodynamic therapy; LED; Periodontitis; Photosensitizer; Porphyrin