Isolation and characterization of a novel putative human polyomavirus.
The small double-stranded DNA polyomaviruses (PyVs) form a family of 73 species, whose natural hosts are primarily mammals and birds. So far, 13 PyVs have been isolated in humans, and some of them have clearly been associated with several diseases, including cancer. In this study, we describe the isolation of a novel PyV in human skin using a sensitive degenerate PCR protocol combined with next-generation sequencing. The new virus, named Lyon IARC PyV (LIPyV), has a circular genome of 5269 nucleotides. Phylogenetic analyses showed that LIPyV is related to the raccoon PyV identified in neuroglial tumours in free-ranging raccoons. Analysis of human specimens from cancer-free individuals showed that 9 skin swabs (9/445; 2.0%), 3 oral gargles (3/140; 2.1%), and one eyebrow hair sample (1/439; 0.2%) tested positive for LIPyV. Future biological and epidemiological studies are needed to confirm the human tropism and provide insights into its biological properties.
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Tarik
T
Gheit
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Sankhadeep
S
Dutta
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Javier
J
Oliver
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Alexis
A
Robitaille
Department of Cancer Epidemiology, Moffitt Cancer Center, Tampa, FL, USA.
Shalaka
S
Hampras
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Jean-Damien
JD
Combes
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Sandrine
S
McKay-Chopin
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Florence
F
Le Calvez-Kelm
Department of Dermatology & Cutaneous Surgery, University of South Florida, Morsani College of Medicine, Tampa, FL, USA; Department of Cutaneous Oncology, Moffitt Cancer Center, Tampa, FL, USA.
Neil
N
Fenske
Department of Dermatology & Cutaneous Surgery, University of South Florida, Morsani College of Medicine, Tampa, FL, USA; Department of Cutaneous Oncology, Moffitt Cancer Center, Tampa, FL, USA.
Basil
B
Cherpelis
Center for Infection Research in Cancer, Moffitt Cancer Center, Tampa, FL, USA.
Anna R
AR
Giuliano
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Silvia
S
Franceschi
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
James
J
McKay
Department of Cancer Epidemiology, Moffitt Cancer Center, Tampa, FL, USA.
Dana E
DE
Rollison
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France. Electronic address: icb@iarc.fr.
Massimo
M
Tommasino
D000595:Amino Acid Sequence; D000818:Animals; D016679:Genome, Viral; D005910:Glioma / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D027601:Polyomavirus Infections / Q000821:virology*; D011821:Raccoons / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins
Human; New polyomavirus; Skin