A distinct bacterial dysbiosis associated skin inflammation in ovine footrot.
Ovine footrot is a highly prevalent bacterial disease caused by Dichelobacter nodosus and characterised by the separation of the hoof horn from the underlying skin. The role of innate immune molecules and other bacterial communities in the development of footrot lesions remains unclear. This study shows a significant association between the high expression of IL1β and high D. nodosus load in footrot samples. Investigation of the microbial population identified distinct bacterial populations in the different disease stages and also depending on the level of inflammation. Treponema (34%), Mycoplasma (29%) and Porphyromonas (15%) were the most abundant genera associated with high levels of inflammation in footrot. In contrast, Acinetobacter (25%), Corynebacteria (17%) and Flavobacterium (17%) were the most abundant genera associated with high levels of inflammation in healthy feet. This demonstrates for the first time there is a distinct microbial community associated with footrot and high cytokine expression.
University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington, United Kingdom.
Grazieli
G
Maboni
University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington, United Kingdom.
Adam
A
Blanchard
Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.|Department of Microbiology, National Veterinary Institute (SVA), Uppsala, Sweden.
Sara
S
Frosth
University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington, United Kingdom.
Ceri
C
Stewart
University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington, United Kingdom.|Advanced Data Analysis Centre (ADAC), University of Nottingham, United Kingdom.
Richard
R
Emes
University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington, United Kingdom.
Sabine
S
Tötemeyer
D000150:Acinetobacter / Q000302:isolation & purification / Q000472:pathogenicity; D000818:Animals; D016022:Case-Control Studies; D060085:Coinfection / Q000378:metabolism / Q000382:microbiology; D020376:Dichelobacter nodosus / Q000472:pathogenicity; D005535:Foot Rot / Q000378:metabolism / Q000382:microbiology*; D053583:Interleukin-1beta / Q000378:metabolism; D064307:Microbiota; D009174:Mycoplasma / Q000302:isolation & purification / Q000472:pathogenicity; D017861:Porphyromonas / Q000302:isolation & purification / Q000472:pathogenicity; D012756:Sheep; D012757:Sheep Diseases / Q000378:metabolism / Q000382:microbiology*; D014208:Treponema / Q000302:isolation & purification / Q000472:pathogenicity
D053583:Interleukin-1beta