Comparative Evaluation of Sensititre YeastOne and CLSI M38-A2 Reference Method for Antifungal Susceptibility Testing of Aspergillus spp. against Echinocandins.
Sensititre YeastOne (YO) panels were assessed forin vitrosusceptibility testing of echinocandins against 39 isolates ofAspergillus fumigatus,A. flavus, andA. terreus, including two echinocandin-resistantA. fumigatusstrains, using different inocula (103, 104, and 105CFU/ml), incubation times (16 to 48 h), and endpoints (first blue or purple well) and compared to CLSI M38-A2. The best agreement was found with an inoculum of 104CFU/ml, incubation times of 20 h forA. flavusand of 30 h forA. fumigatusandA. terreus, and reading the first purple well. The reproducibility within ±1 2-fold dilutions was 100% for all three echinocandins. YO color endpoints were 2 to 3 2-fold dilutions lower than CLSI minimum effective concentrations (MECs) of caspofungin and 1 to 2 2-fold dilutions higher than CLSI MECs of micafungin. For anidulafungin, off-scale YO color endpoints were observed. Nevertheless,A. fumigatusechinocandin-resistant isolates were detected after 24 h of incubation.
Clinical Microbiology Laboratory, Attikon University General Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Maria
M
Siopi
Clinical Microbiology Laboratory, Attikon University General Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Spyros
S
Pournaras
Clinical Microbiology Laboratory, Attikon University General Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece jmeletiadis@med.uoa.gr.|Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands.
Joseph
J
Meletiadis
D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects*; D054714:Echinocandins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000592:standards*; D015203:Reproducibility of Results
D000935:Antifungal Agents; D054714:Echinocandins
Aspergillus spp.; MEC; Sensititre; antifungal susceptibility testing; echinocandins