KRASdetection on archival cytological smears by the novel fully automated polymerase chain reaction-based Idylla mutation test.
BACKGROUND
Molecular techniques are relevant to modern cytopathology, but their implementation is difficult without molecular expertise and infrastructure. The assessment ofKRASmutational status on cytological preparations may be useful either to refine uncertain diagnoses on pancreatic aspirates or to yield predictive information to plan targeted treatment of metastatic colorectal cancer (mCRC). The novel test Idylla™ enables fully automatedKRASgenotyping in approximately 2 h, even in less experienced hands.


METHODS
This study aims to validate this methodology to detectKRASmutations on archival cytological preparations of pancreatic cancer (n= 9) and mCRC (n= 9) by comparing the Idylla™ performance to that of standard real-time polymerase chain reaction.


RESULTS
The same 11 mutations (n= 4: p.G12D;n= 2: p.G12V;n= 2: p.A59E/G/T;n= 1: p.G12R;n= 1: p.G13D;n= 1: p.Q61H) were detected by both techniques.


CONCLUSIONS
Even in less experienced laboratories, a cytopathologist may easily integrate morphological diagnostic report with accurateKRASmutation detection, which is relevant for diagnostic and treatment decisions.
Address: Department of Public Health, University of Naples Federico II, Naples, Italy.
Caterina
C
De Luca
Address: Department of Public Health, University of Naples Federico II, Naples, Italy.
Elena
E
Vigliar
Address: Department of Public Health, University of Naples Federico II, Naples, Italy.
Melania
M
d'Anna
Address: Department of Public Health, University of Naples Federico II, Naples, Italy.
Pasquale
P
Pisapia
Address: Department of Public Health, University of Naples Federico II, Naples, Italy.
Claudio
C
Bellevicine
Address: Department of Public Health, University of Naples Federico II, Naples, Italy.
Umberto
U
Malapelle
Address: Department of Public Health, University of Naples Federico II, Naples, Italy.
Giancarlo
G
Troncone
Aspiration cytology; Idylla; KRAS; molecular pathology