MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars.
A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars 'Peach ND', 'Peach Red', and 'Vivid Scarlet' under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.
Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, Korea.
Eun Sun
ES
Kee
Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, Korea.
Aung Htay
AH
Naing
National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea.
Sun Hyung
SH
Lim
Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, Korea.
Jeung Sul
JS
Han
Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, Korea. ckkim@knu.ac.kr.
Chang Kil
CK
Kim
Anthocyanin; RT-PCR; Spectrophotometer; Transcription factor