Phospholipase Dζ Enhances Diacylglycerol Flux into Triacylglycerol.
Plant seeds are the primary source of triacylglycerols (TAG) for food, feed, fuel, and industrial applications. As TAG is produced from diacylglycerol (DAG), successful engineering strategies to enhance TAG levels have focused on the conversion of DAG to TAG. However, the production of TAG can be limited by flux through the enzymatic reactions that supply DAG. In this study, twoArabidopsis phospholipase Dζgenes (AtPLDζ1andAtPLDζ2) were coexpressed inCamelina sativato test whether the conversion of phosphatidylcholine to DAG impacts TAG levels in seeds. The resulting transgenic plants produced 2% to 3% more TAG as a component of total seed biomass and had increased 18:3 and 20:1 fatty acid levels relative to wild type. Increased DAG and decreased PC levels were examined through the kinetics of lipid assembly by [14C]acetate and [14C]glycerol incorporation into glycerolipids. [14C]acetate was rapidly incorporated into TAG in both wild-type and overexpression lines, indicating a significant flux of nascent and elongated acyl-CoAs into thesn-3 position of TAG. Stereochemical analysis revealed that newly synthesized fatty acids were preferentially incorporated into thesn-2 position of PC, but thesn-1 position of de novo DAG and indicated similar rates of nascent acyl groups into the Kennedy pathway and acyl editing. [14C]glycerol studies demonstrated PC-derived DAG is the major source of DAG for TAG synthesis in both tissues. The results emphasize that the interconversions of DAG and PC pools can impact oil production and composition.
Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (W.Y., G.W., J.L., X.W., D.K.A.); USDA-ARS, Plant Genetics Research Unit, St. Louis, Missouri 63132 (W.Y., D.K.A.); Department of Biology, University of Missouri, St. Louis, Missouri 63121 (G.W., X.W.); and Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406 (P.D.B.).
Wenyu
W
Yang
Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (W.Y., G.W., J.L., X.W., D.K.A.); USDA-ARS, Plant Genetics Research Unit, St. Louis, Missouri 63132 (W.Y., D.K.A.); Department of Biology, University of Missouri, St. Louis, Missouri 63121 (G.W., X.W.); and Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406 (P.D.B.).
Geliang
G
Wang
Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (W.Y., G.W., J.L., X.W., D.K.A.); USDA-ARS, Plant Genetics Research Unit, St. Louis, Missouri 63132 (W.Y., D.K.A.); Department of Biology, University of Missouri, St. Louis, Missouri 63121 (G.W., X.W.); and Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406 (P.D.B.).
Jia
J
Li
Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (W.Y., G.W., J.L., X.W., D.K.A.); USDA-ARS, Plant Genetics Research Unit, St. Louis, Missouri 63132 (W.Y., D.K.A.); Department of Biology, University of Missouri, St. Louis, Missouri 63121 (G.W., X.W.); and Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406 (P.D.B.).
Philip D
http://orcid.org/0000-0002-1291-3363
PD
Bates
Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (W.Y., G.W., J.L., X.W., D.K.A.); USDA-ARS, Plant Genetics Research Unit, St. Louis, Missouri 63132 (W.Y., D.K.A.); Department of Biology, University of Missouri, St. Louis, Missouri 63121 (G.W., X.W.); and Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406 (P.D.B.) swang@danforthcenter.org doug.allen@ars.usda.gov.
Xuemin
http://orcid.org/0000-0002-6251-6745
X
Wang
Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (W.Y., G.W., J.L., X.W., D.K.A.); USDA-ARS, Plant Genetics Research Unit, St. Louis, Missouri 63132 (W.Y., D.K.A.); Department of Biology, University of Missouri, St. Louis, Missouri 63121 (G.W., X.W.); and Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406 (P.D.B.) swang@danforthcenter.org doug.allen@ars.usda.gov.
Doug K
http://orcid.org/0000-0001-8599-8946
DK
Allen
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019607:Brassicaceae / Q000235:genetics / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D024861:Genetic Enhancement / Q000379:methods; D060847:Metabolic Engineering / Q000379:methods; D010713:Phosphatidylcholines / Q000378:metabolism; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
C011439:1,2-diacylglycerol; D029681:Arabidopsis Proteins; D004075:Diglycerides; D005227:Fatty Acids; D010713:Phosphatidylcholines; D014280:Triglycerides; D010739:Phospholipase D; C474583:phospholipase Dzeta1, Arabidopsis; C510354:phospholipase Dzeta2, Arabidopsis