Analysis of phylogeny, distribution, and pathogenicity of Botryosphaeriaceae species associated with gummosis of Anacardium in Brazil, with a new species of Lasiodiplodia.
Netto, M. S. B., Lima, W. G., Correia, K. C., da Silva, C. F. B., Thon, M., Martins, R. B., Miller, R. N. G., Michereff, S. J., and Câmara, M. P. S. 2016. Analysis of phylogeny, distribution, and pathogenicity of Botryosphaeriaceae species associated with gummosis of Anacardium in Brazil, with a new species of Lasiodiplodia. We identified Botryosphaeriaceae species associated with gummosis on Anacardium in Brazil. Isolates were sampled and identified on the basis morphology and phylogeny, through analysis of a partial translation elongation factor 1-α sequence, ribosomal DNA internal transcribed spacers, and β-tubulin gene sequence. Ten taxa were identified, namely, Lasiodiplodia brasiliense, L. euphorbicola, L. gonubiensis, L. iraniensis, L. jatrophicola, L. gravistriata sp. nov., L. pseudotheobromae, L. theobromae, Neofusicoccum batangarum, and Pseudofusicoccum stromaticum. Lasiodiplodia theobromae has been previously reported in cashew and is the most prevalent species observed. All the other species are reported here for the first time on this host. All species of Botryosphaeriaceae were pathogenic on detached green cashew shoots. Differences in aggressiveness were observed among the species, with N. batangarum, L. iraniensis, L. jatrophicola, and L. gravistriata characterized as the most aggressive species, whilst L. euphorbicola and L. pseudotheobromae were identified as the least aggressive.
Departamento de Agronomia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, Pernambuco, Brazil.
Mariote S B
MS
Netto
Departamento de Agronomia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, Pernambuco, Brazil.
Waléria G
WG
Lima
Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, 63133-610 Crato, Ceará, Brazil.
Kamila C
KC
Correia
Embrapa Agroindústria Tropical, 60511-110 Fortaleza, Ceará, Brazil.
Christiana F B
CF
da Silva
Centro Hispano Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, 37185 Villamayor, Spain.
Michael
M
Thon
Campus Arapiraca, Universidade Federal de Alagoas, 57309-005 Arapiraca, Alagoas, Brazil.
Ricardo B
RB
Martins
Departamento de Biologia Celular, Universidade de Brasília, 70910-900 Brasília, D.F., Brazil.
Robert N G
RN
Miller
Departamento de Agronomia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, Pernambuco, Brazil.
Sami J
SJ
Michereff
Departamento de Agronomia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, Pernambuco, Brazil. Electronic address: marcos.camara@ufrpe.br.
Marcos P S
MP
Câmara
D031021:Anacardium / Q000382:microbiology*; D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D001938:Brazil; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D014404:Tubulin
Aggressiveness; Cashew; EF1-α; ITS; Tropical fruit