Potential Protective Effects of Bioactive Constituents from Chinese Propolis against Acute Oxidative Stress Induced by Hydrogen Peroxide in Cardiac H9c2 Cells.
Chinese propolis (CP) is known as a health food but its beneficial effects in protecting cardiomyocytes remain elusive. Here, we investigated the effects of CP and its active compounds on hydrogen peroxide (H2O2) induced rats cardiomyocytes (H9c2) oxidative injury. Cell viability decreases induced by H2O2were mitigated by different CP extracts using various solvents. From these active fractions, six active compounds were separated and identified. Among tested isolated compound, the cytoprotective activities of three caffeates, caffeic acid phenethyl ester (CAPE), benzyl caffeate (BZC), and cinnamyl caffeate (CNC), exerted stronger effects than chrysin, pinobanksin, and 3,4-dimethoxycinnamic acid (DMCA). These three caffeates also increased H9c2 cellular antioxidant potential, decreased intracellular calcium ion ([Ca2+]i) level, and prevented cell apoptosis. Overall, the cardiovascular protective effects of the CP might be attributed to its caffeates constituents (CAPE, BZC, and CNC) and provide evidence for its usage in complementary and alternative medicine.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China.
Liping
0000-0003-0132-8932
L
Sun
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Kai
0000-0003-0553-5293
K
Wang
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China.
Xiang
X
Xu
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China.
Miaomiao
M
Ge
College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Yifan
0000-0003-2628-5764
Y
Chen
College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Fuliang
0000-0002-2457-7475
F
Hu