Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity.
In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH3)2]+) containing 1 mM AgNO3. Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet-visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium.
Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, 515003, Andhra Pradesh, India.
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Gowramma
Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, 515003, Andhra Pradesh, India.
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Keerthi
Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, 515003, Andhra Pradesh, India.
Mokula
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Rafi
Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, 515003, Andhra Pradesh, India. rao.muralidhara@gmail.com.
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Muralidhara Rao
Antimicrobial activity; Diamine silver; EDX; Native strain; SEM; Silver nanoparticles; TEM