miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5.
The present study intended to investigate the biological effects of miR-330-5p on glioblastoma (GBM) cell proliferation and invasiveness by targeting integrin α5 (ITGA5). The expressions of miR-330-5p and ITGA5 mRNA in GBM cell lines (U87, U251, and U373) and normal brain glial cell line (HEB) were detected using RT-qPCR. Protein expression of ITGA5 was examined using Western blot. The present study used MTT assay, colony formation assay, Transwell assay, wound healing assay, and flow cytometry analysis in order to determine the biological functions of GBM cells (including cell proliferation, invasion, migration, apoptosis, and cell cycle). The present study applied dual-luciferase reporter gene assay to identify the target relationship between miR-330-5p and ITGA5. miR-330-5p was low-expressed in GBM cell lines while ITGA5 was high-expressed compared with HEB. miR-330-5p could directly target ITGA5 as well as suppress its expression in GBM cells. Up-regulation of miR-330-5p and down-regulation of ITGA5 both have an inhibitory effect on cell proliferation, invasion, and migration. Meanwhile, they could also promote GBM cell apoptosis. miR-330-5p could suppress proliferation and invasion of GBM cells through targeting ITGA5.
Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.|Department of Neurosurgery, Taixing People's Hospital, Taixing, Jiangsu 225400, China.
Linsen
L
Feng
Department of Neurosurgery, Taixing People's Hospital, Taixing, Jiangsu 225400, China.
Jianhua
J
Ma
Department of Neurosurgery, Taixing People's Hospital, Taixing, Jiangsu 225400, China.
Haiming
H
Ji
Department of Neurosurgery, Taixing People's Hospital, Taixing, Jiangsu 225400, China.
Yichun
Y
Liu
Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China weixing_hu479@sina.com.
Weixing
W
Hu
D017209:Apoptosis; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D015854:Up-Regulation
D039482:Integrin alpha5; C570805:MIRN330 microRNA, human; D035683:MicroRNAs
ITGA5; glioblastoma (GBM); invasiveness; miR-330-5p; proliferation