MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process.
In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3'UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.
Department of Radiotherapy, The Tumor Hospital of Jilin Province, Changchun 130021, China.
Ying
Y
Dong
Department of Thoracic, The Tumor Hospital of Jilin Province, Changchun 130021, China.
Xintian
X
Jin
Department of Invasive Technology, The Tumor Hospital of Jilin Province, Changchun 130021, China.
Zhiqiang
Z
Sun
Department of Oncology, The Tumor Hospital of Jilin Province, Changchun 130021, China.
Yueming
Y
Zhao
Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, China.
Xianjing
X
Song
D020413:3' Untranslated Regions; D000072283:A549 Cells; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*
D020413:3' Untranslated Regions; C533096:MIRN186 microRNA, human; D035683:MicroRNAs
EMT; MiR-186; NSCLC; cdc42; migration