Toll-like receptor 2brightcells identify circulating monocytes in human and non-human primates.
Polychromatic flow cytometry is a useful tool for monitoring circulating whole blood monocytes, although gating strategies often vary depending on the study. Increased analyses of the myeloid system have revealed monocytes to be more plastic than previously understood and uncovered changes among surface markers previously considered to be stable. The myeloid system has also been found to have disparate surface markers between mouse, human, and non-human primate studies, which further complicates examination between species. This study has found bright Toll-like receptor 2 (TLR2) expression to be a consistent surface marker of circulating whole blood monocytes in humans and two species of macaques. Furthermore, within our pigtailed macaque model of HIV-associated CNS disease, where monocyte surface markers have previously been shown to reorganize during acute infection, TLR2 remains stably expressed on the surface of classical, intermediate, and non-classical monocytes. Our findings demonstrate that TLR2 is a useful surface marker for including all monocytes during other phenotypic changes that may alter the expression of common surface receptors. These results provide a practical tool for studying all types of monocytes during inflammation and infection within humans and macaques. © 2017 International Society for Advancement of Cytometry.
Department of Molecular and Comparative Pathobiology, Johns Hopkins School of Medicine, Baltimore, Maryland.
Erin N
EN
Shirk
Division of General Internal Medicine, Department of Medicine, GeneSTAR Research Program, Johns Hopkins School of Medicine, Baltimore, Maryland.|Division of Cardiology, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland.
Brian G
BG
Kral
Department of Molecular and Comparative Pathobiology, Johns Hopkins School of Medicine, Baltimore, Maryland.
Lucio
L
Gama
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D008254:Macaca nemestrina; D009000:Monocytes / Q000378:metabolism*; D051195:Toll-Like Receptor 2 / Q000032:analysis / Q000096:biosynthesis*
D015415:Biomarkers; D051195:Toll-Like Receptor 2
Toll-like receptor 2; flow cytometry; macaca; monocytes