Anti-Inflammatory Activity of the Essential Oil Citral in Experimental Infection withStaphylococcus aureusin a Model Air Pouch.
This study proposes to implement an alternative and effective strategy for local treatment of disease provoked byS. aureus. For the analysis of possible anti-inflammatory activity of essential oil, after establishing an air pouch model, 48 male mice of Balb/c were treated, infected, and euthanized at 4 and 8 h. Thus, the total and differential white blood cells were counted in the animal's blood, and cytokines IL-1β, IL-6, and TNF-αwere titrated using ELISA in the air pouch lavage. Moreover, TNF-α, IL-1β, and IL-6 gene expression was analyzed through an RT-qPCR array, andS. aureuswas quantified using qPCR. Our results,p< 0.05, showed that EOC reduced the quantity of microorganisms. The group of mice treated with essential oil citral showed a significant decrease in TNF-αlevels in tests demonstrating anti-inflammatory activity. There is no data about the mutual influence of the air pouch model, essential oil citral, andS. aureus. Thus, considering the interaction of these variables and the anti-inflammatory activity of the essential oil citral, we demonstrated, by alternative local treatment, a new antimicrobial agent that is not an antibiotic.
State University of Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Ilhéus, BA, Brazil.
Hellen Braga
HB
Martins
State University of Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Ilhéus, BA, Brazil.
Nathan das Neves
ND
Selis
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Clarissa Leal Silva E
CL
Souza
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Flávia S
FS
Nascimento
State University of Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Ilhéus, BA, Brazil.
Suzi Pacheco
SP
de Carvalho
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Lorena D'Oliveira
LD
Gusmão
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Jannine Dos Santos
JD
Nascimento
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Anne Karoline Pereira
AK
Brito
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Samira Itana
SI
de Souza
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Marcio Vasconcelos
MV
de Oliveira
Department of Microbiology, Institute of Biomedical Science, University of São Paulo (USP), São Paulo, SP, Brazil.
Jorge
J
Timenetsky
Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Regiane
R
Yatsuda
State University of Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Ilhéus, BA, Brazil.
Ana Paula T
0000-0003-3992-8474
AP
Uetanabaro
State University of Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Ilhéus, BA, Brazil; Multidisciplinary Institute of Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil.
Lucas M
0000-0002-8276-8149
LM
Marques