Repair of the Orbital Wall Fractures in Rabbit Animal Model Using Nanostructured Hydroxyapatite-Based Implant.
Cellular uptake and cytotoxicity of nanostructured hydroxyapatite (nanoHAp) are dependent on its physical parameters. Therefore, an understanding of both surface chemistry and morphology of nanoHAp is needed in order to be able to anticipate itsin vivobehavior. The aim of this paper is to characterize an engineered nanoHAp in terms of physico-chemical properties, biocompatibility, and its capability to reconstitute the orbital wall fractures in rabbits. NanoHAp was synthesized using a high pressure hydrothermal method and characterized by physico-chemical, structural, morphological, and optical techniques. X-ray diffraction revealed HAp crystallites of 21 nm, while Scanning Electron Microscopy (SEM) images showed spherical shapes of HAp powder. Mean particle size of HAp measured by DLS technique was 146.3 nm. Biocompatibility was estimated by the effect of HAp powder on the adhesion and proliferation of mesenchymal stem cells (MSC) in culture. The results showed that cell proliferation on powder-coated slides was between 73.4% and 98.3% of control cells (cells grown in normal culture conditions). Computed tomography analysis of the preformed nanoHAp implanted in orbital wall fractures, performed at one and two months postoperative, demonstrated the integration of the implants in the bones. In conclusion, our engineered nanoHAp is stable, biocompatible, and may be safely considered for reconstruction of orbital wall fractures.
Ophthalmology Department, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. sanzinici@yahoo.com.
Sinziana
S
Gradinaru
National R & D Institute for Non-ferrous and Rare Metals, 077145 Ilfov, Romania. mpopescu@imnr.ro.
Laura Madalina
LM
Popescu
National R & D Institute for Non-ferrous and Rare Metals, 077145 Ilfov, Romania. roxana@imnr.ro.
Roxana Mioara
RM
Piticescu
Pathology Department, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. sabina_zurac@yahoo.com.
Sabina
S
Zurac
Anatomy Department, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. raduciuluvica@yahoo.com.
Radu
R
Ciuluvica
Institute of Cellular Biology and Pathology "Nicolae Simionescu", 050568 Bucharest, Romania. sanda.burlacu@icbp.ro.
Alexandrina
A
Burlacu
Institute of Cellular Biology and Pathology "Nicolae Simionescu", 050568 Bucharest, Romania. raluca.tutuianu@icbp.ro.
Raluca
R
Tutuianu
National R & D Institute for Non-ferrous and Rare Metals, 077145 Ilfov, Romania. svalsan@imnr.ro.
Sorina-Nicoleta
SN
Valsan
National R & D Institute for Non-ferrous and Rare Metals, 077145 Ilfov, Romania. amotoc@imnr.ro.
Adrian Mihail
AM
Motoc
Ophthalmology Department, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. voineamliliana@yahoo.com.
Liliana Mary
LM
Voinea
implant; mesenchymal stem cells; nanostructured HAp; orbital wall fracture