In vivo evaluation of Mg-6Zn and titanium alloys on collagen metabolism in the healing of intestinal anastomosis.
There is a great clinical need for biodegradable materials, which were used as pins of circular staplers, for gastrointestinal reconstruction in medicine. In this work we compared the effects of the Mg-6Zn and the titanium alloys on collagen metabolism in the healing of the intestinal tract in vivo. The study included Sprague-Dawley rats and their effect was compared on rat's intestinal tract, using serum magnesium, radiology, and immunohistochemistry in vivo. Radiographic and scanning electron microscope evaluation confirmed the degradation by Mg-6Zn alloy during the implantation period. Biochemical measurements including serum magnesium, creatinine, blood urea nitrogen and glutamic-pyruvic-transaminase proved that degradation of Mg-6Zn alloy showed no impact on serum magnesium and the function of other important organs. Superior to titanium alloy, Mg-6Zn alloy enhanced the expression of collagen I/III and relatively suppressed the expression of MMP-1/-13 in the healing tissues, leading to more mature collagen formation at the site of anastomosis. In conclusion, Mg-6Zn alloy performed better than titanium alloy on collagen metabolism and promoted the healing of intestinal anastomosis. Hence, Mg-6Zn may be a promising candidate for use of stapler pins for intestinal reconstruction in the clinically.
Department of Urology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Xiao-Hu
XH
Wang
Department of Urology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Jian-Shu
JS
Ni
Department of Urology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Nai-Long
NL
Cao
Department of General Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Song
S
Yu
Department of General Surgery, Nanjing Medical University Affiliated Wuxi No. 2 People's Hospital, Nanjing 214002, China.
Yi-Gang
YG
Chen
Suzhou Origin Medical Technology Co. Ltd., Suzhou 215513, China.
Shao-Xiang
SX
Zhang
Department of Urology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Bao-Jun
BJ
Gu
Department of General Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Jun
J
Yan
D000497:Alloys / Q000737:chemistry / Q000627:therapeutic use; D000714:Anastomosis, Surgical; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D015415:Biomarkers; D003094:Collagen / Q000378:metabolism*; D007150:Immunohistochemistry; D007422:Intestines / Q000000981:diagnostic imaging / Q000502:physiology* / Q000601:surgery / Q000648:ultrastructure; D011859:Radiography; D051381:Rats; D014025:Titanium / Q000737:chemistry; D014945:Wound Healing
D000497:Alloys; D001672:Biocompatible Materials; D015415:Biomarkers; C000592758:Mg-6Zn alloy; D003094:Collagen; D014025:Titanium