Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP).
Telomere chromatin immunoprecipitation (ChIP) is an experimental method used to determine whether proteins are associated with telomere DNA inside the nuclei of cells and tissues. Telomere-associated proteins are first covalently crosslinked to telomere DNA, and then immunoprecipitated using an antibody specific for the protein of interest. This method has become one of the most indispensable tools for investigating the protein complexes that associate with telomeres.
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, 132 East Waihuan Rd., Higher Education Mega Center, Guangzhou, People's Republic of China.
Feng
F
Liu
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, 132 East Waihuan Rd., Higher Education Mega Center, Guangzhou, People's Republic of China.
Xuyang
X
Feng
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, 132 East Waihuan Rd., Higher Education Mega Center, Guangzhou, People's Republic of China. mawenbin@mail.sysu.edu.cn.
Wenbin
W
Ma
D002843:Chromatin / Q000235:genetics*; D047369:Chromatin Immunoprecipitation / Q000379:methods; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D011485:Protein Binding / Q000235:genetics; D016615:Telomere / Q000235:genetics*
D002843:Chromatin; D004268:DNA-Binding Proteins; D004247:DNA
ChIP; Telomere chromatin immunoprecipitation; Telomere-associated proteins