Dual recognition of chromatin and microtubules by INCENP is important for mitotic progression.
The chromosomal passenger complex (CPC), composed of inner centromere protein (INCENP), Survivin, Borealin, and the kinase Aurora B, contributes to the activation of the mitotic checkpoint. The regulation of CPC function remains unclear. Here, we reveal that in addition to Survivin and Borealin, the single α-helix (SAH) domain of INCENP supports CPC localization to chromatin and the mitotic checkpoint. The INCENP SAH domain also mediates INCENP's microtubule binding, which is negatively regulated by Cyclin-dependent kinase-mediated phosphorylation of segments flanking the SAH domain. The microtubule-binding capacity of the SAH domain is important for mitotic arrest in conditions of suppressed microtubule dynamics, and the duration of mitotic arrest dictates the probability, but not the timing, of cell death. Although independent targeting of INCENP to microtubules or the kinetochore/centromere promotes the mitotic checkpoint, it is insufficient for a robust mitotic arrest. Altogether, our results demonstrate that dual recognition of chromatin and microtubules by CPC is important for checkpoint maintenance and determination of cell fate in mitosis.
Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065.
Michael S
MS
Wheelock
Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065.|Department of Biology, The College of New Jersey, Ewing, NJ 08628.
David J
DJ
Wynne
Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065.|The School of Life Sciences, The University of Nevada Las Vegas, Las Vegas, NV 89154.
Boo Shan
0000-0001-7563-0232
BS
Tseng
Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065 funabih@rockefeller.edu.
Hironori
0000-0003-4831-4087
H
Funabiki
D064107:Aurora Kinase B / Q000378:metabolism; D045744:Cell Line, Tumor; D002503:Centromere / Q000378:metabolism / Q000502:physiology; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism* / Q000502:physiology; D008938:Mitosis / Q000502:physiology*; D010766:Phosphorylation / Q000502:physiology; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D008869:Microtubule-Associated Proteins; D064107:Aurora Kinase B; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases