Distribution of Iron Oxide Core-Titanium Dioxide Shell Nanoparticles in VX2 Tumor Bearing Rabbits Introduced by Two Different Delivery Modalities.
This work compares intravenous (IV) versus fluoroscopy-guided transarterial intra-catheter (IC) delivery of iron oxide core-titanium dioxide shell nanoparticles (NPs) in vivo in VX2 model of liver cancer in rabbits. NPs coated with glucose and decorated with a peptide sequence from cortactin were administered to animals with developed VX2 liver cancer. Two hours after NPs delivery tumors, normal liver, kidney, lung and spleen tissues were harvested and used for a series on histological and elemental analysis tests. Quantification of NPs in tissues was done both by bulk inductively coupled plasma mass spectrometry (ICP-MS) analysis and by hard X-ray fluorescence microscopy. Both IV and IC NPs injection are feasible modalities for delivering NPs to VX2 liver tumors with comparable tumor accumulation. It is possible that this is an outcome of the fact that VX2 tumors are highly vascularized and hemorrhagic, and therefore enhanced permeability and retention (EPR) plays the most significant role in accumulation of nanoparticles in tumor tissue. It is, however, interesting to note that IV delivery led to increased sequestration of NPs by spleen and normal liver tissue, while IC delivery lead to more NP positive Kupffer cells. This difference is most likely a direct outcome of blood flow dynamics. Armed with this knowledge about nanoparticle delivery, we plan to test them as radiosensitizers in the future.
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. tamerabdelrhman2012@u.northwestern.edu.|Clinical Oncology Department and Pathology Department, Faculty of Medicine, Alexandria University, Alexandria 21131, Egypt. tamerabdelrhman2012@u.northwestern.edu.
Tamer
T
Refaat
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. derek.west@northwestern.edu.
Derek
D
West
Clinical Oncology Department and Pathology Department, Faculty of Medicine, Alexandria University, Alexandria 21131, Egypt. samarelachy@yahoo.com.
Samar
S
El Achy
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. v-parini@northwestern.edu.
Vamsi
V
Parimi
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. jasmine.may@northwestern.edu.
Jasmine
J
May
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. LunXin2015@u.northwestern.edu.
Lun
L
Xin
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. k-r-harris@northwestern.edu.
Kathleen R
KR
Harris
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. will.c.liu@gmail.com.
William
W
Liu
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. m-wanzer@northwestern.edu.
Michael Beau
MB
Wanzer
X-ray Sciences Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA. lfinney@aps.anl.gov.
Lydia
L
Finney
X-ray Sciences Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA. emaxey@aps.anl.gov.
Evan
E
Maxey
X-ray Sciences Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA. svogt@aps.anl.gov.
Stefan
S
Vogt
Department of Radiology and Radiological Sciences, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. reed.omary@vanderbilt.edu.
Reed A
RA
Omary
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. d-procissi@northwestern.edu.
Daniele
D
Procissi
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. a-larson@northwestern.edu.
Andrew C
AC
Larson
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. tpaunesku@northwestern.edu.
Tatjana
T
Paunesku
Radiation Oncology Department, Radiology Department and Pathology Core Facility, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. g-woloschak@northwestern.edu.
Gayle E
GE
Woloschak
core–shell nanoparticle; rabbit VX2 liver cancer model; transarterial intra-catheter delivery