Dysbiosis and compositional alterations with aging in the gut microbiota of patients with heart failure.
Emerging evidence has suggested a potential impact of gut microbiota on the pathophysiology of heart failure (HF). However, it is still unknown whether HF is associated with dysbiosis in gut microbiota. We investigated the composition of gut microbiota in patients with HF to elucidate whether gut microbial dysbiosis is associated with HF. We performed 16S ribosomal RNA gene sequencing of fecal samples obtained from 12 HF patients and 12 age-matched healthy control (HC) subjects, and analyzed the differences in gut microbiota. We further compared the composition of gut microbiota of 12 HF patients younger than 60 years of age with that of 10 HF patients 60 years of age or older. The composition of gut microbial communities of HF patients was distinct from that of HC subjects in both unweighted and weighted UniFrac analyses. Eubacterium rectale and Dorea longicatena were less abundant in the gut microbiota of HF patients than in that of HC subjects. Compared to younger HF patients, older HF patients had diminished proportions of Bacteroidetes and larger quantities of Proteobacteria. The genus Faecalibacterium was depleted, while Lactobacillus was enriched in the gut microbiota of older HF patients. These results suggest that patients with HF harbor significantly altered gut microbiota, which varies further according to age. New concept of heart-gut axis has a great potential for breakthroughs in the development of novel diagnostic and therapeutic approach for HF.
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Takehiro
T
Kamo
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Hiroshi
http://orcid.org/0000-0002-3574-9607
H
Akazawa
Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.|Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan.
Wataru
W
Suda
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Akiko
A
Saga-Kamo
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Yu
Y
Shimizu
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Hiroki
H
Yagi
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Qing
Q
Liu
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Seitaro
S
Nomura
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Atsuhiko T
AT
Naito
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Norifumi
N
Takeda
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Mutsuo
M
Harada
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Haruhiro
H
Toko
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.|Department of Advanced Clinical Science and Therapeutics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Hidetoshi
H
Kumagai
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Yuichi
Y
Ikeda
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Eiki
E
Takimoto
Department of Advanced Clinical Science and Therapeutics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Jun-Ichi
JI
Suzuki
Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan.|RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
Kenya
K
Honda
Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
Hidetoshi
H
Morita
Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan.|Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Masahira
M
Hattori
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Issei
I
Komuro
D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D041963:Bacteroidetes / Q000235:genetics; D016022:Case-Control Studies; D064806:Dysbiosis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D006333:Heart Failure / Q000382:microbiology*; D006801:Humans; D008297:Male; D064307:Microbiota / Q000235:genetics; D008875:Middle Aged; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S