Endothelial long non-coding RNAs regulated by oxidized LDL.
Oxidized low-density lipoprotein (oxLDL) plays a central role in the pathogenesis of atherosclerosis, in part via an effect to promote endothelial dysfunction. In the present study, we evaluated the expression profiles of long non-coding RNAs (lncRNAs) and protein-coding mRNAs in endothelial cells following oxLDL stimulation. LncRNAs and mRNAs from human umbilical vein endothelial cells (HUVECs) were profiled with the Arraystar Human lncRNA Expression Microarray V3.0 following 24 h of oxLDL treatment (100 µg/mL). Of the 30,584 lncRNAs screened, 923 were significantly up-regulated and 975 significantly down-regulated (P < 0.05) in response to oxLDL exposure. In the same HUVEC samples, 518 of the 26,106 mRNAs screened were up-regulated and 572 were down-regulated. Of these differentially expressed lncRNAs, CLDN10-AS1 and CTC-459I6.1 were the most up-regulated (~87-fold) and down-regulated (~28-fold), respectively. Bioinformatic assignment of the differentially regulated genes into functional groups indicated that many are involved in signaling pathways among which are the cytokine receptor, chemokine, TNF, MAPK and Ras signaling pathways, olfactory transduction, and vascular smooth muscle cell function. This is the first report profiling oxLDL-mediated changes in lncRNA and mRNA expression in human endothelial cells. The novel targets revealed substantially extend the list of potential candidate genes involved in atherogenesis.
Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada. singhk@smh.ca.|Division of Vascular Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, 5E21 KRCBS, 30 Bond Street, Toronto, ON, M5B 1W8, Canada. singhk@smh.ca.|Division of Endocrinology & Metabolism, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, Canada. singhk@smh.ca.|Department of Surgery, University of Toronto, Toronto, ON, Canada. singhk@smh.ca.
Krishna K
KK
Singh
Division of Cardiology, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, Canada.
Pratiek N
PN
Matkar
Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada.
Yi
Y
Pan
Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada.
Adrian
A
Quan
Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada.
Vijay
V
Gupta
Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada.|Division of Endocrinology & Metabolism, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, Canada.
Hwee
H
Teoh
Division of Vascular Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, 5E21 KRCBS, 30 Bond Street, Toronto, ON, M5B 1W8, Canada.|Department of Surgery, University of Toronto, Toronto, ON, Canada.|Department of Surgery, King Saud University and the King Saud University-Li Ka Shing Collaborative Research Program, Riyadh, Saudi Arabia.
Mohammed
M
Al-Omran
Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, M5B 1W8, Canada. vermasu@smh.ca.|Department of Surgery, University of Toronto, Toronto, ON, Canada. vermasu@smh.ca.|Department of Pharmacology & Toxicology, University of Toronto, Toronto, ON, Canada. vermasu@smh.ca.
Subodh
S
Verma
D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000378:metabolism*
D008077:Lipoproteins, LDL; D062085:RNA, Long Noncoding; C099252:oxidized low density lipoprotein
Endothelial cells; LncRNA; OxLDL