Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome Footprinting.
Quantitatively detecting correlations of multiple protein-protein interactions (PPIs) in vivo is a big challenge. Here we introduce a novel method, termed Protein-interactome Footprinting (PiF), to simultaneously measure multiple PPIs in one cell. The principle of PiF is that each target physical PPI in the interactome is simultaneously transcoded into a specific DNA sequence based on dimerization of the target proteins fused with DNA-binding domains. The interaction intensity of each target protein is quantified as the copy number of the specific DNA sequences bound by each fusion protein dimers. Using PiF, we quantitatively reveal dynamic patterns of PPIs and their correlation network in E. coli two-component systems.
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science &Technology of China, Hefei, China.
Si-Wei
SW
Luo
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science &Technology of China, Hefei, China.
Zhi
Z
Liang
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science &Technology of China, Hefei, China.|School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Jia-Rui
JR
Wu
D001665:Binding Sites; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D023302:Protein Footprinting / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D055503:Protein Multimerization
D029968:Escherichia coli Proteins