Involvement of testicular DAAM1 expression in zinc protection against cadmium-induced male rat reproductive toxicity.
In order to verify the effects of exposure to Cd and Zn on testicular DAAM1 gene and protein expression and also to ascertain their involvement in the protective role of Zn in prevent the testicular toxicity Cd-induced in male offspring rats at adult age after gestational and lactational exposure, male offspring rats, from mothers receiving either tap water, Cd, Zn, or Cd + Zn during gestation and lactation periods, were scarified on postnatal days (PND) 70. The reproductive organ (testis, epididymis, and vesicle seminal) were collected, weighed, and analyzed. The results showed that exposure to Cd in utero and through lactation decreased the relative reproductive organ weight, altered the testicular histology at the interstitial and tubular levels, and causing a significant reduction in the daily sperm production (DSP) per testis and per gram of testis, and other then altering the epididymal sperm quality. Furthermore, both mRNA and protein expression of rat testicular DAAM1 were also inhibited in Cd-treated group. Zn supply has completely corrected the most of these toxic effects. Our results imply that Zn could prevent Cd-induced testicular toxicity and sperm quality alteration in adult male rat after gestational and lactational exposure, probably via the restoration of the testicular DAAM1 expression inhibited by Cd.
LR11ES41: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, Université de Monastir, Monastir, Tunisia.
Marouane
M
Chemek
Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate "F. Bottazzi", Università della Campania Luigi Vanvitelli, Napoli, Italy.
Massimo
M
Venditti
LR11ES41: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, Université de Monastir, Monastir, Tunisia.
Sana
S
Boughamoura
LR11ES41: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, Université de Monastir, Monastir, Tunisia.
Safa B
SB
Mimouna
LR11ES41: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, Université de Monastir, Monastir, Tunisia.
Imed
I
Messaoudi
Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate "F. Bottazzi", Università della Campania Luigi Vanvitelli, Napoli, Italy.
Sergio
http://orcid.org/0000-0002-1007-7840
S
Minucci
D000367:Age Factors; D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D019610:Cytoprotection; D003598:Cytoskeletal Proteins; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007774:Lactation; D008297:Male; D018811:Maternal Exposure; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D017967:Zinc Compounds / Q000494:pharmacology*
D002712:Chlorides; D003598:Cytoskeletal Proteins; C000606312:Daam1 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D020011:Protective Agents; D012333:RNA, Messenger; D017967:Zinc Compounds; C016837:zinc chloride; D019256:Cadmium Chloride
DAAM1; cadmium; gestation and lactation; sperm; testis; zinc