Glycine post-synthetic modification of MIL-53(Fe) metal-organic framework with enhanced and stable peroxidase-like activity for sensitive glucose biosensing.
A facile and rapid post-synthetic strategy was proposed to prepare a glycine functionalized MIL-53(Fe), namely glycine-MIL-53(Fe), by a simple mixing of water dispersible MIL-53(Fe) and glycine. The FT-IR, SEM, XRD and zeta potential were used to characterize the glycine-MIL-53(Fe). The result showed that glycine post-synthetic modification of MIL-53(Fe) did not change in the morphology and crystal structure of MIL-53(Fe). Interestingly, compared with MIL-53(Fe), the glycine-MIL-53(Fe) exhibits an enhanced peroxidase-like activity, which could catalyze the oxidation of TMB by H2O2to produce an intensive color reaction. Kinetic analysis indicated that the Kmof glycine-MIL-53(Fe) for TMB was one-tenth of that of MIL-53(Fe). The glycine-MIL-53(Fe) as peroxidase mimetic displays better stability under alkaline or acidic conditions than MIL-53(Fe). The good performance of glycine-MIL-53(Fe) over MIL-53(Fe) may be attributed to the increase of affinity between TMB and the glycine-MIL-53(Fe). With these characteristics, a simple and sensitive method was developed for the detection of H2O2and glucose. The linear detection range for H2O2is 0.10-10μM with a detection limit of 49nM, and glucose could be linearly detected in the range from 0.25 to 10μM with a detection limit of 0.13μM. The proposed method was successfully used for glucose detection in human serum samples.
The Key Laboratory of Luminescence and Real-time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Wenfei
W
Dong
The Key Laboratory of Luminescence and Real-time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Liaoyuan
L
Yang
The Key Laboratory of Luminescence and Real-time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuminghuang2000@yahoo.com.
Yuming
Y
Huang
D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis; D005947:Glucose / Q000032:analysis*; D005998:Glycine / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D057230:Limit of Detection; D000073396:Metal-Organic Frameworks / Q000138:chemical synthesis / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism*
D001786:Blood Glucose; D000073396:Metal-Organic Frameworks; D007501:Iron; D009195:Peroxidase; D005947:Glucose; D005998:Glycine
Biosensing; Enhanced peroxidase-like activity; Glucose; Glycine modification; Metal–organic framework