Classico-molecular targeting of oligopeptidase B, cysteine protease and variable surface glycoprotein (VSG) genes ofTrypanosoma evansi.
Trypanosomosis or Surra can rightly be attributed as the most economically important vector-borne haemoprotozoan disease encountering India. Surra infected chronic cases show almost similar types of signs and symptoms often confusing it with other haemoprotozoan infections, thereby, making it prerequisite for the development of aspecific and sensitive technique for its detection in susceptible animals. Blood microscopy and serology suffers from the hands of lack of sensitivity and specificity thereby leaving molecular detection techniques as one of the promising alternative. Alongside, there is utmost need for exploring of new molecular gene targets for the development of a putative alternative for diagnosis and immunoprophylaxsis. The present communication describes the identification and amplification of oligopeptidase B, cysteine protease and variable surface glycoprotein genes ofT. evansiso as to exploit them in future as potential candidates for immune protection and/or molecular detection.
Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001 India.
Ruchi Singh
RS
Gaur
Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001 India.
Vikrant
V
Sudan
Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001 India.
Amit Kumar
AK
Jaiswal
Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001 India.
Amit
A
Singh
Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001 India.
Daya
D
Shanker
Cysteine protease; Oligopeptidase B; Trypanosoma evansi; Variable surface glycoprotein