Minicircle classes heterogeneity within the TcIII and TcIV discrete typing units of Trypanosoma cruzi.
The taxon Trypanosoma cruzi, causative agent of Chagas disease, is composed of several discrete typing units (DTUs) named TcI-TcVI, and Tcbat. The history of the taxon T. cruzi is known, even though several controversial aspects remain as the relationships between TcIII and TcIV. We analyzed cloned T. cruzi stocks pertaining to the seven DTUs by filter hybridization tests of PCR amplicons from minicircle variable regions and kinetoplast DNA probes. Minicircle DNA blots from the cloned stocks and filter hybridization with one TcI, one TcII, one TcV, one TcVI, three TcIII, one TcIV from North America and one TcIV kinetoplast DNA probes from South America revealed minicircle variable region cross-reaction in some T. cruzi DTUs probed. TcIII was heterogeneous in minicircle class composition, even though two TcIII probes revealed that a small fraction of minicircles cross-hybridized with the minicircles from the TcIII, TcV and TcVI DTUs. The minicircles of TcIV from North America cross-reacted only with TcIV from North America but not with TcIV stocks from Brazil and Bolivia. The results on minicircle cross-hybridizations are discussed in the context of RNA editing, mitochondrial function in T. cruzi DTUs.
Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
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Ortiz
Programa de Microbiología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
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Osorio
Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile. Electronic address: asolari@med.uchile.cl.
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Solari
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology; D003001:Cloning, Molecular; D018105:DNA, Kinetoplast / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D058889:Molecular Typing; D009656:North America; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000235:genetics*; D013020:South America; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D018105:DNA, Kinetoplast; D016053:RNA, Protozoan
TcIII; TcIV; Trypanosoma cruzi; kDNA minicircles