Teratogenic responses of zebrafish embryos to decabromodiphenyl ether (BDE-209) in the presence of nano-SiO2particles.
This study investigated the influence of nano-SiO2particles (nSiO2) on the teratogenic responses of zebrafish embryos to decabromodiphenyl ether (BDE-209). Zebrafish embryos were exposed to BDE-209 in the absence and presence of nSiO2for 96 h post fertilization (hpf). Results showed that formation of nSiO2-BDE-209 associates promoted both extracellular and intracellular uptake of BDE-209 by zebrafish embryos, thereby increasing the bioconcentration of BDE-209 on the chorion surface and the embryos. Results also showed embryos delay hatching temporarily when co-exposure to BDE-209 and nSiO2at 60 hpf. Furthermore, there was heartbeat decline (28.3 beats/10s) and increase in irregular heartbeat (45.8%) in zebrafish larvae at 96 hpf, compared to the sole exposure to BDE-209 (32.7 beats/10s and 0%). Malformation in terms of spinal curvature (SC), pericardial edema (PE) and yolk sac edema (YSE) were observed on zebrafish larvae at 33.9, 23.4, and 18%, respectively. Overall, abnormal development of zebrafish was apparent when co-exposure to BDE-209 and nSiO2. All relevant evidence considered, nSiO2could facilitate the transport of BDE-209 towards zebrafish embryos and negatively impact the development of zebrafish.
Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan.
Shu-Ju
SJ
Chao
Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan; Department of Civil and Environmental Engineering, University of Delaware, Newark, DE 19716, USA.
Chin Pao
CP
Huang
Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan.
Pei-Chung
PC
Chen
Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan. Electronic address: cphuang@mail.nctu.edu.tw.
Chihpin
C
Huang
D000015:Abnormalities, Multiple / Q000139:chemically induced; D000818:Animals; D004337:Drug Carriers / Q000494:pharmacology; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D055768:Halogenated Diphenyl Ethers / Q000493:pharmacokinetics / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D007814:Larva / Q000254:growth & development; D053758:Nanoparticles; D012822:Silicon Dioxide / Q000494:pharmacology*; D013723:Teratogens / Q000494:pharmacology*; D015027:Zebrafish / Q000196:embryology* / Q000254:growth & development
D004337:Drug Carriers; D055768:Halogenated Diphenyl Ethers; D013723:Teratogens; D012822:Silicon Dioxide; C010902:decabromobiphenyl ether
Bioconcentration; Decabromodiphenyl ether; Nano-SiO(2) particles; Teratogenic responses; Zebrafish embryos