Potential application of waste from castor bean (Ricinus communis L.) for production for xylanase of interest in the industry.
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds-1using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6.0, after 120 h of fermentation. The influence of PEG molar mass (1000-8000 g mol-1), phase concentrations (PEG 20.0-24.0 % w/w and sodium citrate 15-20 % w/w) and pH (6.0-8.0) on partition coefficient, purification factor, yield and selectivity of XY were determinate. Enzyme partitioning into the PEG rich phase was favored by MPEG8000 (g mol-1), CPEG24 % (w/w), CC20 % (w/w) and pH 8.0, resulting in partition coefficient of 50.78, activity yield of 268 %, 7.20-fold purification factor and selectivity of 293. A. japonicus URM5620 has a potential role in the development of a bioprocess for the XY production using low-cost media. In addition, the present study proved it is feasible to extract xylanase from SSF by adopting the one step ATPS consisting of PEG/citrate.
Mycology Department, Federal University of Pernambuco, Recife, PE, Brazil.|Animal Morphology and Physiology Department, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s/n, Recife, PE, 52171-900, Brazil.
Polyanna Nunes
PN
Herculano
Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Garanhuns, PE, Brazil.
Keila Aparecida
KA
Moreira
Animal Morphology and Physiology Department, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s/n, Recife, PE, 52171-900, Brazil. rpbezerra@yahoo.com.br.
Raquel Pedrosa
RP
Bezerra
Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Garanhuns, PE, Brazil.
Tatiana Souza
TS
Porto
Mycology Department, Federal University of Pernambuco, Recife, PE, Brazil.
Cristina Maria
CM
de Souza-Motta
Animal Morphology and Physiology Department, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s/n, Recife, PE, 52171-900, Brazil.
Ana Lúcia Figueiredo
AL
Porto
Aqueous two phase systems; Aspergillus japonicus; Production; Solid-state fermentation; Xylanase