Determination of Polycyclic Aromatic Hydrocarbons in Commercial Parenteral Formulations and Medications Using High-Performance Liquid Chromatography with Diode Array Detection.
HPLC coupled to UV diode array detection (DAD) is proposed for the determination of polycyclic aromatic hydrocarbons (PAHs) in pharmaceutical products for parenteral administration. Because rubber is a possible source of PAHs for these products, samples stored in containers with rubber parts were selected for the analysis. The basis for method optimization was EPA Method 8310, which determines 16 priority PAHs in ground water and wastewater by HPLC using both UV and fluorescence detection. Using DAD, two channels were selected for detection, with one operating at 254 nm for the detection of nine PAHs and the other at 225 nm for the detection of seven PAHs. This method allowed for the detection of PAHs using external calibration with LODs and LOQs ranging from 0.001 to 0.060 &#x03BC;g/mL and from 0.003 to 0.167 &#x03BC;g/mL, respectively. Within-day precision, expressed as RSD, varied from 1.24 to 7.76% for PAH concentrations from 0.05 to 0.50 &#x03BC;g/mL, and intraday precision varied from 3.10 to 9.40% for the same concentration range. Method accuracy was confirmed by recoveries of 75&#x2013;120% of the spiked samples. This method was applied for the determination of PAHs in three commercial infusion solutions and in nine different medications stored in syringes prior to administration to patients. Twelve of 16 PAHs were found in these samples. Total PAH concentrations varied from 0.13 to 13.50 &#x03BC;g/mL. Pyrene was the most prevalent contaminant, being present in 11 of 12 samples in concentrations ranging from 0.17 to 4.80 &#x03BC;g/mL. This method presented good sensitivity for the measurement of PAH in the target samples, allowing for the determination of the 16 priority PAHs in one run and in 30 min.
Federal University of Santa Maria, Postgraduate Program in Pharmaceutical Sciences, Avenida Roraima 1000, Santa Maria 97105-900, Brazil.
Marcia M
MM
Barichello
Federal University of Santa Maria, Postgraduate Program in Pharmaceutical Sciences, Avenida Roraima 1000, Santa Maria 97105-900, Brazil.
Denise
D
Bohrer
Federal University of Santa Maria, Postgraduate Program in Pharmaceutical Sciences, Avenida Roraima 1000, Santa Maria 97105-900, Brazil.
Carine
C
Viana
Federal University of Santa Maria, Department of Chemistry, Avenida Roraima 1000, Santa Maria, 97105-900, Brazil.
Leandro M
LM
Carvalho
Federal University of Santa Maria, Department of Chemistry, Avenida Roraima 1000, Santa Maria, 97105-900, Brazil.
Paulo C
PC
Nascimento