Biological Effect of Licochalcone C on the Regulation of PI3K/Akt/eNOS and NF-κB/iNOS/NO Signaling Pathways in H9c2 Cells in Response to LPS Stimulation.
Polyphenols compounds are a group molecules present in many plants. They have antioxidant properties and can also be helpful in the management of sepsis. Licochalcone C (LicoC), a constituent ofGlycyrrhiza glabra, has various biological and pharmacological properties. In saying this, the effect of LicoC on the inflammatory response that characterizes septic myocardial dysfunction is poorly understood. The aim of this study was to determine whether LicoC exhibits anti-inflammatory properties on H9c2 cells that are stimulated with lipopolysaccharide. Our results have shown that LicoC treatment represses nuclear factor-κB (NF-κB) translocation and several downstream molecules, such as inducible nitric oxide synthase (iNOS), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Moreover, LicoC has upregulated the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/endothelial nitric oxide synthase (eNOS) signaling pathway. Finally, 2-(4-Morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride (LY294002), a specific PI3K inhibitor, blocked the protective effects of LicoC. These findings indicate that LicoC plays a pivotal role in cardiac dysfunction in sepsis-induced inflammation.
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti 66100, Italy. s.franceschelli@unich.it.
Sara
S
Franceschelli
Department of Psychological, Health and Territorial Sciences, University "G. D'Annunzio", Chieti 66100, Italy. alessio.ferrone@yahoo.it.
Mirko
M
Pesce
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti 66100, Italy. danielagatta@unich.it.
Alessio
A
Ferrone
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti 66100, Italy. antonia.patruno@unich.it.
Daniela Maria Pia
DM
Gatta
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti 66100, Italy. maria.delutiis@unich.it.
Antonia
A
Patruno
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti 66100, Italy. mfelaco@unich.it.
Maria Anna De
MA
Lutiis
Department of Physiology, Institute of Nutrition and Food Technology "José Mataix", Biomedical Research Centre, University of Granada, Granada 18071, Spain. lorenza.speranza@unich.it.
José Luis
JL
Quiles
Department of Psychological, Health and Territorial Sciences, University "G. D'Annunzio", Chieti 66100, Italy. mirkopesce@unich.it.
Alfredo
A
Grilli
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti 66100, Italy. algrilli@unich.it.
Mario
M
Felaco
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti 66100, Italy. jlquiles@ugr.es.
Lorenza
L
Speranza
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D047188:Chalcones / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D047188:Chalcones; D018799:Intercellular Adhesion Molecule-1; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; C000600260:licochalcone C
Akt; adhesion molecule; cardiomyocytes; inflammation; licochalcone C; nitric oxide; nuclear factor-κB