A DNA Logic Gate Automaton for Detection of Rabies and Other Lyssaviruses.
Immediate activation of biosensors is not always desirable, particularly if activation is due to non-specific interactions. Here we demonstrate the use of deoxyribozyme-based logic gate networks arranged into visual displays to precisely control activation of biosensors, and demonstrate a prototype molecular automaton able to discriminate between seven different genotypes of Lyssaviruses, including Rabies virus. The device uses novel mixed-base logic gates to enable detection of the large diversity of Lyssavirus sequence populations, while an ANDNOT logic gate prevents non-specific activation across genotypes. The resultant device provides a user-friendly digital-like, but molecule-powered, dot-matrix text output for unequivocal results read-out that is highly relevant for point of care applications.
Division of Experimental Therapeutics, Department of Medicine, Columbia University, 630 W 168thSt, New York, NY, 10032, USA.
Pavithra
P
Vijayakumar
Division of Experimental Therapeutics, Department of Medicine, Columbia University, 630 W 168thSt, New York, NY, 10032, USA.|Genecology Research Centre; Inflammation and Healing Research Cluster, School of Science and Engineering, University of the Sunshine Coast, Queensland, Australia.
Joanne
http://orcid.org/0000-0003-4624-3545
J
Macdonald
D001331:Automation; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D039301:Computers, Molecular; D021881:DNA, Catalytic / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D008128:Logic; D018114:Lyssavirus / Q000235:genetics / Q000302:isolation & purification*; D011820:Rabies virus / Q000235:genetics / Q000302:isolation & purification*
D021881:DNA, Catalytic; D004279:DNA, Viral
DNA logic gate; biosensors; molecular automaton; viral diagnostics; visual display