Antimicrobial, Antioxidant, and Anticancer Activities of Biosynthesized Silver Nanoparticles Using Marine Algae Ecklonia cava.
Green synthesis of silver nanoparticles (AgNPs) has gained great interest as a simple and eco-friendly alternative to conventional chemical methods. In this study, AgNPs were synthesized by using extracts of marine algaeEcklonia cavaas reducing and capping agents. The formation of AgNPs using aqueous extract ofEcklonia cavawas confirmed visually by color change and their surface plasmon resonance peak at 418 nm, measured by UV-visible spectroscopy. The size, shape, and morphology of the biosynthesized AgNPs were observed by transmission electron microscopy and dynamic light scattering analysis. The biosynthesized AgNPs were nearly spherical in shape with an average size around 43 nm. Fourier transform-infrared spectroscopy (FTIR) analysis confirmed the presence of phenolic compounds in the aqueous extract ofEcklonia cavaas reducing and capping agents. X-ray diffraction (XRD) analysis was also carried out to demonstrate the crystalline nature of the biosynthesized AgNPs. Antimicrobial results determined by an agar well diffusion assay demonstrated a significant antibacterial activity of the AgNPs againstEscherichia coliandStaphylococcus aureus. Antioxidant results determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay revealed an efficient antioxidant activity of the biosynthesized AgNPs. The biosynthesized AgNPs also exhibited a strong apoptotic anticancer activity against human cervical cancer cells. Our findings demonstrate that aqueous extract ofEcklonia cavais an effective reducing agent for green synthesis of AgNPs with efficient antimicrobial, antioxidant, and anticancer activities.
Division of Bioengineering, Incheon National University, Incheon 406-772, Korea. venkatjchem@gmail.com.
Jayachandran
J
Venkatesan
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea. sknkim@pknu.ac.kr.
Se-Kwon
SK
Kim
Division of Bioengineering, Incheon National University, Incheon 406-772, Korea. msshim@inu.ac.kr.
Min Suk
MS
Shim
Ecklonia cava; anticancer; antimicrobial; antioxidant; biosynthesis; nanoparticle