Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination.
Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen- or fixed-section processing. Initial studies have described imaging of breast tissue using fluorescence confocal microscopy with small regions of interest, typically 750 × 750 ?? ? m 2 . We present exploration with a microscope, termed confocal strip-mosaicking microscope (CSM microscope), which images an area of 2 × 2 ?? cm 2 of tissue with cellular-level resolution in 10 min of excision. Using the CSM microscope, we imaged 34 fresh, human, large breast tissue specimens from 18 patients, blindly analyzed by a board-certified pathologist and subsequently correlated with the corresponding standard fixed histopathology. Invasive tumors and benign tissue were clearly identified in CSM strip-mosaic images. Thirty specimens were concordant for image-to-histopathology correlation while four were discordant.
Memorial Sloan Kettering Cancer Center, Dermatology Service, New York, New York, United States.
Sanjee
S
Abeytunge
Memorial Sloan Kettering Cancer Center, Dermatology Service, New York, New York, United StatesbDrew University, Physics Department, Madison, New Jersey, United States.
Bjorg
B
Larson
Memorial Sloan Kettering Cancer Center, Dermatology Service, New York, New York, United States.
Gary
G
Peterson
Memorial Sloan Kettering Cancer Center, Breast Service, New York, New York, United States.
Monica
M
Morrow
Memorial Sloan Kettering Cancer Center, Dermatology Service, New York, New York, United States.
Milind
M
Rajadhyaksha
Memorial Sloan Kettering Cancer Center, Breast Pathology, New York, New York, United States.
Melissa P
MP
Murray
D001940:Breast / Q000000981:diagnostic imaging*; D001941:Breast Diseases / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D018613:Microscopy, Confocal