Anti-hyperglycaemic activity ofMoringa oleiferais partly mediated by carbohydrase inhibition and glucose-fibre binding.
Moringa oleiferahas potential anti-hyperglycaemic effects that have been reported earlier by different scientific groups using animal models of diabetes. We aimed to explore the possible mechanisms of action ofM. oleiferaextract through different methods. Primarily, we measured fasting blood glucose and performed glucose tolerance test, in Type 2 diabetic rats. Further, we studied the effects of extracts on pancreatic insulin concentration. Extracts' effect on carbohydrate breakdown was assayed using α-amylase inhibition assays and assay of six different segments of gastrointestinal (GI) tracts. Anin situintestinal perfusion model and a glucose fibre assay were performed to see the potentiality ofM. oleiferaon glucose absorption.M. oleiferashowed no significant change in insulin secretionin vivoAdditionally, substantial effect of the extract was seen on retarded glucose absorption and in thein situperfusion study of rat intestinal model. α-amylase action was inhibited by the extract, yet again, these findings were further confirmed via the Six Segment assay, where sucrose digestion was found to be inhibited throughout the length of the GI tract. A combinedin vitro,in vivoandin situtests justified the potential of anti-hyperglycaemic activity ofM. oleiferaand its tissue level mechanism is also justified.
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka 1229, Bangladesh.
Sohel Bin
SB
Azad
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka 1229, Bangladesh chemist89ansari@gmail.com.
Prawej
P
Ansari
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka 1229, Bangladesh.
Shofiul
S
Azam
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka 1229, Bangladesh.
Saad Mosharraf
SM
Hossain
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka 1229, Bangladesh.
Mohammad Ibtida-Bin
MI
Shahid
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka 1229, Bangladesh.
Mahmudul
M
Hasan
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka 1229, Bangladesh.
J M A
JMA
Hannan
D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007328:Insulin / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D029588:Moringa oleifera / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D020318:Rats, Long-Evans
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D006026:Glycoside Hydrolases; C019822:carbohydrase; D005947:Glucose
ELISA; glucose tolerance; hypoglycemia; type 2 diabetes