Synthesis of a GlcNAcylated arginine building block for the solid phase synthesis of death domain glycopeptide fragments.
Herein we describe the synthesis of glycopeptide fragments from the death domains of TRADD and FADD bearing the recently discovered Nω-GlcNAc-β-arginine post-translational modification. TRADD and FADD glycopeptides were accessed through the use of a suitably protected synthetic glycosylamino acid 'cassette' that could be directly incorporated into conventional solid phase peptide synthesis (SPPS) protocols.
School of Chemistry, The University of Sydney, Sydney 2006, Australia.
Siyao
S
Wang
School of Chemistry, The University of Sydney, Sydney 2006, Australia.
Leo
L
Corcilius
ACRF Chemical Biology Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, VIC 3052, Australia.
Phillip P
PP
Sharp
School of Chemistry, The University of Sydney, Sydney 2006, Australia. Electronic address: richard.payne@sydney.edu.au.
Richard J
RJ
Payne
D001120:Arginine / Q000737:chemistry*; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D060327:Solid-Phase Synthesis Techniques
D006020:Glycopeptides; D001120:Arginine
Arginine; FADD; GlcNAcylation; Glycopeptides; Glycosylation; SPPS; TRADD