G Protein-Coupled Receptor 30 (GPR30) Expression Pattern in Inflammatory Bowel Disease Patients Suggests its Key Role in the Inflammatory Process. A Preliminary Study.
OBJECTIVE
G protein-coupled receptor 30 (GPR30) is a recently de-orphanized estrogen receptor that mediates the effects of estrogens on different cells. It has been postulated that in inflammatory bowel diseases (IBD) activation of GPR30 blocks the pathways dependent on pro-inflammatory cytokines. The aim of our study was to investigate GPR30 expression in patients with IBD and its potential implication in future therapies.


METHODS
Fifty-seven patients were enrolled in our study: 20 subjects with Crohn's disease (CD), 22 with ulcerative colitis (UC) and 15 controls. In each subject, biopsies were taken from various left-colonic locations. Gene and protein expression of GPR30 was quantified using real time RT-PCR or Western blot.


RESULTS
GPR30 mRNA and protein expression were detected in all tested colonic tissues. No significant differences in GPR30 gene expression were observed. In non-inflamed areas, GPR30 protein was strongly increased in CD patients, but moderately in UC patients (p= 0.014 and p=0.143, respectively, vs. controls). In CD patients, a significantly lower GPR30 protein content in inflamed than in non-inflamed tissue was observed (p=0.039). The change was independent of patient gender.


CONCLUSIONS
Our observations indicate that GPR30 may play a role in the development and progression of inflammatory lesions in IBD, thus affecting disease severity, and consequently IBD treatment. Therefore, GPR30 may become an attractive target for novel anti-IBD drugs, particularly in CD.
Department of Biochemistry, Medical University of Lodz, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland. dr.mwlodarczyk@gmail.com.
Marcin
M
Włodarczyk
Department of Biochemistry, Medical University of Lodz;Department of Gastroenterology,Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Aleksandra
A
Sobolewska-Włodarczyk
Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Adam I
AI
Cygankiewicz
Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Damian
D
Jacenik
Department of Biochemistry, Medical University of Lodz;Department of Gastroenterology,Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Aleksandra
A
Piechota-Polańczyk
Department of Biochemistry, Medical University of Lodz;Department of Gastroenterology,Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Krystyna
K
Stec-Michalska
Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Wanda M
WM
Krajewska
Department of Biochemistry, Medical University of Lodz, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Jakub
J
Fichna
Department of Biochemistry, Medical University of Lodz;Department of Gastroenterology,Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Maria
M
Wiśniewska-Jarosińska
D000328:Adult; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D003424:Crohn Disease / Q000235:genetics / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010865:Pilot Projects; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D055815:Young Adult
C109584:GPER1 protein, human; D018836:Inflammation Mediators; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled