DNA Aptamers for the Characterization of Histological Structure of Lung Adenocarcinoma.
Nucleic acid aptamers are becoming popular as molecular probes for identification and imaging pathology and, at the same time, as a convenient platform for targeted therapy. Recent studies have shown that aptamers may be effectively used for tumor characterization and as commercially available monoclonal antibodies. Here we present three DNA aptamers binding to whole transformed lung cancer tissues, including tumor cells, connective tissues, and blood vessels. Protein targets have been revealed using affinity purification followed by mass spectrometry analyses, and they have been validated using a panel of correspondent antibodies and 3D imaging of tumor tissues. Each of the proteins targeted by the aptamers is involved in cancer progression and most of them are crucial for lung adenocarcinoma. We propose the use of these aptamers in aptahistochemistry for the characterization of the histological structure of lung adenocarcinoma. The value of the presented aptamers is their application together or separately for indicating the spread of neoplastic transformation, for complex differential diagnostics, and for targeted therapy of the tumor itself as well as all transformed structures of the adjacent tissues. Moreover, it has been demonstrated that these aptamers could be used for intraoperative tumor visualization and margin assessment.
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia; Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, Krasnoyarsk 660036, Russia.
Galina S
GS
Zamay
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia.
Tatiana I
TI
Ivanchenko
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia.
Tatiana N
TN
Zamay
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia.
Valentina L
VL
Grigorieva
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia.
Yury E
YE
Glazyrin
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia; Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, Krasnoyarsk 660036, Russia.
Olga S
OS
Kolovskaya
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia; Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, Krasnoyarsk 660036, Russia.
Irina V
IV
Garanzha
OPTEC Group, Novosibirsk 630090, Russia.
Andrey A
AA
Barinov
Krasnoyarsk Regional Clinical Cancer Center, Krasnoyarsk 660022, Russia.
Alexey V
AV
Krat
Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Gleb G
GG
Mironov
Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Ana
A
Gargaun
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia; Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, Krasnoyarsk 660036, Russia.
Dmitry V
DV
Veprintsev
Krasnoyarsk Regional Clinical Pathological Anatomical Bureau, Krasnoyarsk 660022, Russia.
Sergey S
SS
Bekuzarov
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia.
Andrey K
AK
Kirichenko
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia; Krasnoyarsk Regional Clinical Cancer Center, Krasnoyarsk 660022, Russia.
Ruslan A
RA
Zukov
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia.
Marina M
MM
Petrova
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia; Krasnoyarsk Regional Clinical Cancer Center, Krasnoyarsk 660022, Russia.
Andrey A
AA
Modestov
Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada. Electronic address: maxim.berezovski@uottawa.ca.
Maxim V
MV
Berezovski
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia; Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, Krasnoyarsk 660036, Russia. Electronic address: aszamay@gmail.com.
Anna S
AS
Zamay
DNA-aptamer; histological structure; lung adenocarcinoma