Lignin from bamboo shoot shells as an activator and novel immobilizing support for α-amylase.
This study examined the feasibility of α-amylase activation and immobilization, using lignin from bamboo shoot shells (BSS). Our results demonstrated that BSS lignin is an excellent α-amylase activator and it elevated α-amylase activity more than two-fold at a concentration of 5mg/ml. For immobilization of α-amylase via adsorption, BSS lignin was incubated in an α-amylase solution (5mg/ml) for 20min, and the maximum specific activity, amount of loaded protein and activity recovery were 92.4U/mg, 19.0mg/g and 111%, respectively. In contrast to its free counterpart, immobilized α-amylase improved the catalytic efficiency and storage stability, under comparable working conditions (temperature and pH). Regarding its convenient usage, immobilized enzyme can be suspended in advance, but a suspension incubated at 60°C should be used within 30min. The residual activity after 14 re-uses remained at a reasonable level (53.2%). In conclusion, this study reveals a novel support for enzyme immobilization.
College of Food Science, Southwest University, Chongqing 400715, People's Republic of China; Normal College of Jishou University, Jishou 416000, People's Republic of China.
Weihua
W
Gong
College of Food Science, Southwest University, Chongqing 400715, People's Republic of China.
Zhanxiang
Z
Ran
College of Food Science, Southwest University, Chongqing 400715, People's Republic of China.
Fayin
F
Ye
College of Food Science, Southwest University, Chongqing 400715, People's Republic of China; Chongqing Engineering Research Centre of Regional Foods, Chongqing 400716, People's Republic of China. Electronic address: zhaogh@swu.edu.cn.
Guohua
G
Zhao
D031723:Bambusa / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry*; D000516:alpha-Amylases / Q000378:metabolism*
D004800:Enzymes, Immobilized; D008031:Lignin; D000516:alpha-Amylases
Activator; Bamboo shoot shell; Immobilized; Lignin; α-Amylase