Synthesis of naproxen-imprinted polymer using Pickering emulsion polymerization.
For the last decades, molecular imprinting is developing intensively, especially in the case of the application of new imprinting techniques. In this work, for the first time, a Pickering emulsion polymerization was used to synthesize the S-naproxen-imprinted polymer spheres following a noncovalent protocol. To enhance the knowledge about imprinting process using mentioned technique, thorough analysis of the synthesis process was performed. Optimization of polymerization conditions included the selection of functional monomer, cross-linking agent, type of porogen, surfactant, and the choice of appropriate amount of the template and porogen. Prepared materials were characterized using scanning electron microscopy and nitrogen adsorption. To study the binding properties, the sorption studies, including adsorption isotherms and competitive binding, were performed. Investigation of the effect of the functional monomer on the selective recognition of S-naproxen showed that the interactions between the template molecule and 4-vinylpyridine resulted in the best recognizing ability. Moreover, the synthesis with application of ethylene glycol dimethacrylae as a cross-linker, toluene as a porogen, and Tween 20 as an additional emulsion stabilizer gave the most desired result. The optimal ratio of the porogen to monomers mixture was 0.1, due to the fact that the increase of the porogen volume resulted in the significant increase of nonspecific uptake. In addition, the tenfold molar excess of functional monomer relative to the template turned out to be optimal. Subsequent binding studies demonstrated that the material synthesized using optimized polymerization conditions consists of imprinted sites that are sensitive for the S-naproxen.
Faculty of Chemistry, Wrocław University of Science and Technology, Wrocław, Poland.|Division of Pure and Applied Biochemistry, Lund University, Lund, Sweden.
Małgorzata
0000-0003-0721-1487
M
Kujawska
Division of Pure and Applied Biochemistry, Lund University, Lund, Sweden.
Tongchang
T
Zhou
Faculty of Chemistry, Wrocław University of Science and Technology, Wrocław, Poland.
Andrzej W
AW
Trochimczuk
Division of Pure and Applied Biochemistry, Lund University, Lund, Sweden.
Lei
L
Ye
D000327:Adsorption; D006801:Humans; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D009288:Naproxen / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use; D009584:Nitrogen; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry
D011108:Polymers; D011725:Pyridines; D009288:Naproxen; C029351:4-vinylpyridine; D009584:Nitrogen
MIP; Pickering emulsion; Triton X-100; Tween 20