A novel versatile microbiosensor for local hydrogen detection by means of scanning photoelectrochemical microscopy.
The development of a versatile microbiosensor for hydrogen detection is reported. Carbon-based microelectrodes were modified with a [NiFe]-hydrogenase embedded in a viologen-modified redox hydrogel for the fabrication of a sensitive hydrogen biosensor By integrating the microbiosensor in a scanning photoelectrochemical microscope, it was capable of serving simultaneously as local light source to initiate photo(bio)electrochemical reactions while acting as sensitive biosensor for the detection of hydrogen. A hydrogen evolution biocatalyst based on photosystem 1-platinum nanoparticle biocomplexes embedded into a specifically designed redox polymer was used as a model for proving the capability of the developed hydrogen biosensor for the detection of hydrogen upon localized illumination. The versatility and sensitivity of the proposed microbiosensor as probe tip allows simplification of the set-up used for the evaluation of complex electrochemical processes and the rapid investigation of local photoelectrocatalytic activity of biocatalysts towards light-induced hydrogen evolution.
Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Fangyuan
F
Zhao
Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Felipe
F
Conzuelo
Plant Biochemistry, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Volker
V
Hartmann
Center for Electrochemical Sciences - Molecular Nanostructures, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Huaiguang
H
Li
Center for Electrochemical Sciences - Molecular Nanostructures, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Stefanie
S
Stapf
Plant Biochemistry, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Marc M
MM
Nowaczyk
Plant Biochemistry, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Matthias
M
Rögner
Center for Electrochemical Sciences - Molecular Nanostructures, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Nicolas
N
Plumeré
Max Planck Institut für Chemische Energiekonversion, Stiftstrasse 34-36, D-45470 Mülheim an der Ruhr, Germany.
Wolfgang
W
Lubitz
Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany. Electronic address: wolfgang.schuhmann@rub.de.
Wolfgang
W
Schuhmann
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D006859:Hydrogen / Q000302:isolation & purification* / Q000378:metabolism; D006864:Hydrogenase / Q000737:chemistry; D008853:Microscopy / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
D011108:Polymers; D002244:Carbon; D006859:Hydrogen; C043296:nickel-iron hydrogenase; D006864:Hydrogenase
Hydrogen biosensor; Hydrogenase; Oxygen-protection shield; PS1-Pt; SECM; Viologen-modified redox polymer