Cutaneous applied nano-ZnO reduce the ability of hair follicle stem cells to differentiate.
The ability of metal oxide nanoparticles to penetrate the skin has aroused a great deal of interest during the past decade due to concerns over the safety of topically applied sunscreens that contain physical UV-resistant metal particles, such as nano-Zinc oxide (nZnO). Previous studies demonstrate that metal oxide nanoparticles accumulate in skin furrows and hair follicles following topical application while little is known about the consequence of these nanoparticles on skin homeostasis. The current investigation tested the effects of nZnO (0.5 mg/day mouse) on hair follicle physiology. Topical application of Vaseline containing nZnO, bulk ZnO (bZnO), or ionized Zn to newborn mice vibrissa pad over a period of 7 consecutive days revealed that nZnO accumulated within hair follicles, and this induced the apoptosis of hair follicle stem cells (HFSCs). In vitro studies also indicated that nZnO exposure caused obvious DNA damage and induced apoptosis in HFSCs. Furthermore, it was found that nZnO exposure perturbed genes associated with HFSC apoptosis, cell communication, and differentiation. HFSCs transplantation assay demonstrated that the potential of HFSCs to differentiate was reduced. This investigation indicates a potential risk of topically applied ZnO nanoparticles on skin homeostasis.
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Wei
W
Ge
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.|b College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , Qingdao , Shandong , China.
Yong
Y
Zhao
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Fang-Nong
FN
Lai
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Jing-Cai
JC
Liu
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Yuan-Chao
YC
Sun
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Jun-Jie
JJ
Wang
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Shun-Feng
SF
Cheng
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Xi-Feng
XF
Zhang
c Reproductive Center, Weifang City People's Hospital , Weifang , Shandong , China.
Li-Lan
LL
Sun
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Lan
L
Li
d Department of Animal Sciences , Auburn University , Auburn , AL , USA.
Paul W
PW
Dyce
a Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University , Qingdao , Shandong , China.
Wei
W
Shen
D000279:Administration, Cutaneous; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D004249:DNA Damage; D018859:Hair Follicle / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D053758:Nanoparticles / Q000633:toxicity*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D012869:Skin Absorption; D013234:Stem Cells / Q000187:drug effects* / Q000473:pathology; D013473:Sunscreening Agents / Q000493:pharmacokinetics / Q000633:toxicity*; D015034:Zinc Oxide / Q000493:pharmacokinetics / Q000633:toxicity*
D013473:Sunscreening Agents; D015034:Zinc Oxide
Nano-ZnO particles; RNA seq; cytotoxicity; differentiation potential; hair follicle stem cell