Putative model based on iTRAQ proteomics for Spirulina morphogenesis mechanisms.
The morphology of Spirulina during cultivation is susceptible to external interferences, but the morphogenesis mechanism is still unclear. Here the proteomic changes of linear Spirulina and spiral Spirulina were comparatively investigated via isobaric tag for relative and absolute quantitation (iTRAQ). Totally 165 and 167 differences in proteins expression were screened out from the TJSD2/TJSD3 and TJBC4-1/TJBC4-2 groups, respectively. Gene ontology and metabolic pathway analysis of differences in proteins expression uncovered the metabolic pathways (photosynthesis, carbon fixation, sugar metabolism) that were significantly enriched with the proteins correlated with Spirulina morphogenesis. The results of differences in proteins expression in metabolic pathway were verified by quantitative real-time PCR. We also built a putative model of Spirulina morphogenesis mechanism and thought multiple metabolic pathways interact and take part in Spirulina morphogenesis.
Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Fu-Shuang
FS
Wang
Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Shi-Rui
SR
Dong
Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Hong-Yu
HY
Zhang
Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. Electronic address: wsying@tjcu.edu.cn.
Su-Ying
SY
Wang
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D063990:Gene Ontology; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D053545:Spirulina / Q000254:growth & development / Q000378:metabolism*
D001426:Bacterial Proteins; D020543:Proteome
Morphogenesis; Proteomics; Putative model; Spirulina