Comparative subproteomic analysis of clinically acquired fluoroquinolone resistance and ciprofloxacin stress in Salmonella Typhimurium DT104B.
Antimicrobial resistance is a worldwide public health threat and Salmonella enterica subsp. enterica serotype Typhimurium phage type DT104B multiresistant strains with additional quinolone resistance have been responsible for global outbreaks and high mortality. Quinolone resistance is known to be multifactorial but is still far from a complete understanding. To give new insights about the resistance mechanisms involved, this work aimed to evaluate subproteome changes between an S. Typhimurium DT104B clinical strain that acquired fluoroquinolone resistance after treatment (Se20) and its pretreatment parental strain (Se6), and also subproteome variations in Se20 under ciprofloxacin (CIP) stress (Se20+CIP).



The proteomes were compared at the intracellular and membrane levels by a 2-DE∼LC-MS/MS and a shotgun LC-MS/MS approach, respectively.



In total, 14 differentially abundant proteins were identified when comparing Se6 with Se20 and 91 were identified between Se20 and Se20+CIP. Several proteins with known and possible roles in quinolone resistance (AAC(6')-Ib-cr4, OmpD, OmpX, GlmS, GlmU, H-NS, etc.) were identified and discussed.



The great number of proteins identified in this study provides important information about mechanism-related differential protein expression which supports the current knowledge and might lead to new testable hypotheses on the mechanism of action of fluoroquinolone drugs.
Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Veterinary Science Department, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|UCIBIO-REQUIMTE, Faculty of Science and Technology, Nova University of Lisbon, Lisbon, Portugal.
Susana
S
Correia
Institut National de la Recherche Agronomique (INRA), Université Clermont Auvergne (UCA), UMR Microbiologie Environnement Digestif et Santé (MEDiS), site de Theix, France.|Institut National de la Recherche Agronomique (INRA), UR370 QuaPA, Plate-Forme d'Exploration du Métabolisme composante protéomique, site de Theix, France.
Michel
M
Hébraud
Institut National de la Recherche Agronomique (INRA), Université Clermont Auvergne (UCA), UMR Microbiologie Environnement Digestif et Santé (MEDiS), site de Theix, France.
Ingrid
I
Chafsey
Institut National de la Recherche Agronomique (INRA), UR370 QuaPA, Plate-Forme d'Exploration du Métabolisme composante protéomique, site de Theix, France.
Christophe
C
Chambon
Institut National de la Recherche Agronomique (INRA), UR370 QuaPA, Plate-Forme d'Exploration du Métabolisme composante protéomique, site de Theix, France.
Didier
D
Viala
Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, Logrono, Spain.
Yolanda
Y
Sáenz
UCIBIO-REQUIMTE, Faculty of Science and Technology, Nova University of Lisbon, Lisbon, Portugal.|ProteoMass Scientific Society, Faculty of Sciences and Technology, Caparica Campus, Caparica, Portugal.
José Luis
JL
Capelo
Veterinary Science Department, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|UCIBIO-REQUIMTE, Faculty of Science and Technology, Nova University of Lisbon, Lisbon, Portugal.
Patrícia
P
Poeta
Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|UCIBIO-REQUIMTE, Faculty of Science and Technology, Nova University of Lisbon, Lisbon, Portugal.
Gilberto
0000-0002-6365-0735
G
Igrejas
D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D013312:Stress, Physiological / Q000187:drug effects*
D002939:Ciprofloxacin
Antimicrobial resistance; Ciprofloxacin stress; Comparative subproteomics; Fluoroquinolone resistance; Salmonella Typhimurium DT104B