Evaluation of the pharmacokinetics of imipenem following regional limb perfusion using the saphenous and the cephalic veins in standing horses.
This prospective experimental study goal was to determine the pharmacokinetics of imipenem after intravenous regional limb perfusion (IV-RLP) in standing horses. Nine horses participated in the study; that was approved by the University Animal Care and Use Committee. One thoracic limb or one pelvic limb of each horse was randomly selected. After the veins were catheterized, an Esmarch bandage tourniquet was applied and the catheter was injected with a solution containing 500mg of imipenem. Synovial fluid samples were collected from the fetlock joint and blood samples were collected from the jugular vein. All samples were analyzed for imipenem concentration using liquid chromatography mass spectrometry. Cmax of imipenem in the fetlock joint using the cephalic and the saphenous vein was 87 and 60μg⁄mL, respectively. The results indicate that by performing IV-RLP using the cephalic/saphenous, one can achieve imipenem concentrations in the fetlock joint that are well above the MIC of most susceptible pathogens including resistant bacteria such as Methicillin Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. Thus, with selective; judicious use, RLP with imipenem can markedly increase treatment efficacy of severe distal limb infections in horses.
Large Animal Department, Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel. Electronic address: gal.kelmer@mail.huji.ac.il.
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Kelmer
Large Animal Department, Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
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Tatz
Large Animal Department, Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
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Kdoshim
The National Residue Control Laboratory, Kimron Veterinary Institute, Bet Dagan, Israel.
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Britzi
Small Animal Department, Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
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Segev
D061605:Administration, Intravenous / Q000662:veterinary; D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D004333:Drug Administration Routes / Q000662:veterinary; D005260:Female; D005552:Forelimb / Q000098:blood supply* / Q000378:metabolism*; D006736:Horses / Q000378:metabolism*; D015378:Imipenem / Q000493:pharmacokinetics*; D008297:Male; D010477:Perfusion; D011446:Prospective Studies; D013582:Synovial Fluid / Q000737:chemistry
D000900:Anti-Bacterial Agents; D015378:Imipenem
Antimicrobials; Horse; Imipenem; Pharmacokinetics; Regional limb perfusion