Effects of insulin and the glucagon-like peptide 1 receptor agonist liraglutide on the kidney proteome in db/db mice.
Diabetes mellitus (DM) is a worldwide disease that affects 9% of the adult world population and type 2 DM accounts for 90% of those. A common consequence of DM is kidney complications, which could lead to kidney failure. We studied the potential effects of treatment with insulin and the glucagon-like peptide 1 receptor (GLP-1R) agonist liraglutide on the diabetic kidney proteome through the use of the db/db mouse model system and mass spectrometry (MS). Multivariate analyses revealed distinct effects of insulin and liraglutide on the db/db kidney proteome, which was seen on the protein levels of, for example, pterin-4α-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor-1α(PCBD1), neural precursor cell expressed developmentally down-regulated-8 (NEDD8), transcription elongation factor-B polypeptide-1 (ELOC) and hepcidin (HEPC). Furthermore, the separation of the insulin, liraglutide and vehicle db/db mouse groups in multivariate analyses was not mainly related to the albumin excretion rate (AER) or the level of glycated hemoglobin A1c(HbA1c%) in the mice. In summary, we show that insulin and liraglutide give rise to separate protein profiles in the db/db mouse kidney.
Department of Immunotechnology, Lund University, Lund, Sweden leena.liljedahl@immun.lth.se.
Leena
L
Liljedahl
Novo Nordisk A/S, Måløv, Denmark.
Jenny
J
Norlin
Novo Nordisk A/S, Måløv, Denmark.
James N
JN
McGuire
Department of Immunotechnology, Lund University, Lund, Sweden.
Peter
P
James
D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D000075065:Elongin / Q000378:metabolism; D064451:Hepcidins / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D000069450:Liraglutide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D000075687:NEDD8 Protein / Q000378:metabolism; D009765:Obesity / Q000378:metabolism; D020543:Proteome / Q000187:drug effects*
C000616411:Eloc protein, mouse; D000075065:Elongin; D064451:Hepcidins; D007004:Hypoglycemic Agents; D007328:Insulin; D000075687:NEDD8 Protein; C513172:Nedd8 protein, mouse; D020543:Proteome; D000069450:Liraglutide; D006836:Hydro-Lyases; C076392:pterin-4a-carbinolamine dehydratase
GLP‐1R agonist liraglutide; OPLS‐DA; insulin; kidney proteome