Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture.
The commercial success of tissue engineering products requires efficacy, cost effectiveness, and the possibility of scaleup. Advances in tissue engineering require increased sophistication in the design of biomaterials, often challenging the current manufacturing techniques. Interestingly, several of the properties that are desirable for biomaterial design are embodied in the structure and function of plants. This study demonstrates that decellularized plant tissues can be used as adaptable scaffolds for culture of human cells. With simple biofunctionalization technique, it is possible to enable adhesion of human cells on a diverse set of plant tissues. The elevated hydrophilicity and excellent water transport abilities of plant tissues allow cell expansion over prolonged periods of culture. Moreover, cells are able to conform to the microstructure of the plant frameworks, resulting in cell alignment and pattern registration. In conclusion, the current study shows that it is feasible to use plant tissues as an alternative feedstock of scaffolds for mammalian cells.
Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Gianluca
G
Fontana
Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA.
Joshua
J
Gershlak
Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Michal
M
Adamski
Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Jae-Sung
JS
Lee
Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA.
Shion
S
Matsumoto
Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Hau D
HD
Le
Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Bernard
B
Binder
Olbrich Botanical Gardens, Madison, WI, 53704, USA.
John
J
Wirth
Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, 01609, USA.
Glenn
G
Gaudette
Biomedical Engineering, Material Sciences and Engineering, Department of Orthopedics and Rehabilitation, Department of Surgery, University of Wisconsin-Madison, Madison, WI, 53705, USA.
William L
WL
Murphy
D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D006801:Humans; D032426:Marantaceae / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D028528:Petroselinum / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
biofunctionalization; biomaterials; cell expansion; decellularized scaffolds; plants