Chloroform-free permeabilization for improved detection of β-galactosidase activity in Vibrio cholerae.
LacZ (β-galactosidase) is used to monitor the transcription of genes in reporter strains carrying the lacZ gene under the control of a promotor of interest. This protocol for LacZ activity determinations in Vibrio cholerae following detergent lysis results in 2.5-fold increase of LacZ activities compared to lysis with chloroform.
Institute of Microbiology, University of Hohenheim (Stuttgart), 70599 Stuttgart, Germany.
Charlotte
C
Toulouse
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Claudia C
CC
Häse
Institute of Microbiology, University of Hohenheim (Stuttgart), 70599 Stuttgart, Germany. Electronic address: julia.steuber@uni-hohenheim.de.
Julia
J
Steuber
D001426:Bacterial Proteins / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D002725:Chloroform / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D017930:Genes, Reporter; D007763:Lac Operon; D009596:Nitrophenols / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011480:Protease Inhibitors / Q000494:pharmacology; D014158:Transcription, Genetic; D014734:Vibrio cholerae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D003902:Detergents; D009596:Nitrophenols; D011480:Protease Inhibitors; D002725:Chloroform; D001616:beta-Galactosidase
Bacterial cell lysis; LacZ; Vibrio cholerae; β-Galactosidase