Highly photosensitive colorimetric immunoassay for tumor marker detection based on Cu2+doped Ag-AgI nanocomposite.
Visual detection of tumor markers with accuracy and selectivity exhibits significant advantages for cancer early diagnosis due to its low-cost and simple analytical instruments. Herein, a highly photosensitive colorimetric immunoassay for carcino embryonie antigen (CEA, as a model) detection based on Cu2+doped Ag-AgI nanocomposite was constructed. First, Cu2+doped Ag-AgI nanocomposite (Cu2+/Ag-AgI) owned the ability of oxidization of TMB was synthesized by impregnation method, which was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD), UV-Vis diffuse reflectance spectroscopy (DRS) and X-ray photoelectron spectroscopy (XPS). Second, based on sandwich-type immunoassay, the immunocomplex was built on the surface of magnetic bead by utilizing Cu2+/Ag-AgI as labels. The blue color of TMBoxwas obtained under visible irradiation and ultraviolet spectrum scanning was carried out in ABS buffer solution. Under optimal conditions, the absorbance values increased with the increasing of CEA concentrations in sample with the linearity range of 1.0pgmL-1-5.0ngmL-1. The detection limit was 0.13pgmL-1. More importantly, the developed colorimetric immunoassay exhibited good selectivity, repeatability, stability and possible applications in the real serum sample analysis.
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: zhangbing01@tyut.edu.cn.
Bing
B
Zhang
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Xue
X
Wang
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China; Medical Imaging Department, Shanxi Medical University, Taiyuan 030001, China.
Yanling
Y
Zhao
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Jia
J
Lv
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Hongyun
H
Meng
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Honghong
H
Chang
Medical Imaging Department, Shanxi Medical University, Taiyuan 030001, China. Electronic address: yxyxxx@126.com.
Hui
H
Zhang
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Wenlong
W
Wei
D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood; D003124:Colorimetry / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007454:Iodides / Q000737:chemistry*; D008027:Light; D053761:Nanocomposites / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry*
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D007454:Iodides; D018030:Silver Compounds; D012834:Silver; D003300:Copper; C030584:silver iodide
Ag-AgI; Colorimetric; Cu(2+); Immunoassay; Photoresponsive