Irisin acts as a regulator of macrophages host defense.
OBJECTIVE
Irisin, the adipomyokine, released mainly by exercised muscles, participate in the browning of adipose tissue and contribute to the restriction of insulin resistance and diabetes mellitus 2 development. Because of the limited reports describing the effect of irisin on inflammation and immunocompetent cells activation, the present study attempted to assess the influence of various irisin concentrations on basic macrophage activity.


METHODS
Studies were carried out on murine RAW 264.7 macrophages cultured in medium enriched with irisin (0nM, 10nM, 50nM, or 100nM). General cell activity, viability, and proliferation were assessed along with phagocytosis process, and respiratory burst generation.


RESULTS
Irisin level positively correlates with general cell activity and cell cycle progression as well as with phagocytosis intensity, but negatively correlates with the intensity of respiratory burst generation. No influence of irisin on quiescent cell viability, including apoptosis or necrosis, was observed.


CONCLUSIONS
This research is the first to show that irisin modulates macrophage activity by reducing reactive oxygen species (ROS) overproduction, which could suggest its potential anti-inflammatory properties. Therefore, further studies are needed for the evaluation of influence of irisin on immunocompetent cell function.
Department of Ergonomics and Exercise Physiology, Faculty of Health Science, Jagiellonian University Medical College, Grzegorzecka 20, 31-531 Krakow, Poland. Electronic address: agnieszka.mazur@uj.edu.pl.
Agnieszka Irena
AI
Mazur-Bialy
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D005353:Fibronectins / Q000276:immunology / Q000494:pharmacology*; D007249:Inflammation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D000067996:RAW 264.7 Cells; D017382:Reactive Oxygen Species / Q000276:immunology*; D016897:Respiratory Burst / Q000187:drug effects* / Q000276:immunology
C571428:FNDC5 protein, mouse; D005353:Fibronectins; D017382:Reactive Oxygen Species
Immune responses; Inflammation; Irisin; Macrophage; Phagocytosis; Physical activity