Rapid differentiating colorectal cancer and colorectal polyp using dried blood spot mass spectrometry metabolomic approach.
Colorectal cancer (CRC) is the third leading causes of cancer mortality, and the early-stage detection could significantly enhance survival rates. Cancer influences the important metabolic pathways and the changes in metabolite levels had been used in many studies as the potential biomarkers. This study is aimed at screening metabolite biomarkers with CRC diagnosis potentials. The direct infusion mass spectrometry (MS) metabolomic analysis based on dried blood spot was used to distinguish CRC from polyp. The target metabolites were composed of 23 amino acids and 26 acylcarnitines. The 21 metabolites in blood were selected via multivariate analysis. A regression model was established based on parameters C16, Arg, C4/C8, C5/C3, Val, Phe/Tyr, Ala, C4/C3. Tenfold cross validation (CV) method was used to test this model and showed sensitivity of 81.18% and specificity of 83.95%. The metabolomic analysis is a practicable method for CRC detection. The use of direct MS analysis in metabolite screening could be finished in several minutes and served as a higher-throughput method to distinguish CRC and polyps. © 2017 IUBMB Life, 69(5):347-354, 2017.
Jinzhou Medical University, Jinzhou, Liaoning, China.|The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.|Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.
Yu
Y
Jing
The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.|Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.
Xue
X
Wu
Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.|Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian.|Clinical Laboratory, Dalian Sixth People's Hospital, Dalian, People's Republic of China.
Peng
P
Gao
Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.|Department of Toxicology, School of Public Health, Tianjin Medical University, Heping District, Tianjin, China.
Zhongze
Z
Fang
Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.|Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian.
Jingjing
J
Wu
The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.|Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.
Qingjun
Q
Wang
The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.|Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.
Chen
C
Li
The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.|Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.
Zhitu
Z
Zhu
Key Laborotary of Liaoning Tumor Clinical Metabolomics (KLLTCM), Jinzhou, Liaoning, China.|Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian.|Key Laboratory of Contraceptives and Devices Research (NPFPC), Shanghai Engineer and Technology Research Center of Reproductive Health Drug and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai.
Yunfeng
Y
Cao
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000097:blood / Q000378:metabolism; D002331:Carnitine / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D003111:Colonic Polyps / Q000175:diagnosis*; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D059788:Dried Blood Spot Testing / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D015203:Reproducibility of Results
D000596:Amino Acids; C116917:acylcarnitine; D002331:Carnitine
colorectal cancer; mass spectrometry; metabolomics