Evaluation of antibacterial activity of the bark and leaf extracts of Brosimum gaudichaudii Trécul against multidrug resistant strains.
Brosimum gaudichaudii Trécul., a plant that belongs to Moraceae family, is found throughout the Brazilian Cerrado. The antimicrobial activities of ethanolic bark and leaf extracts of B. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (DFIs). Antimicrobial activity of the extracts was evaluated by agar disc diffusion (DD) and broth dilution (BD) methods. By BD method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial activity against both gram-negative and gram-positive bacteria. Increased antimicrobial activity was observed when bark and leaf extracts were tested against Staphylococcus aureus (63.64%) and Pseudomonas aeruginosa (66.67%). Statistical analyses of bark and leaf extract demonstrated antimicrobial activity against both gram-positive (p = 0.000) and gram-negative bacteria (p = 0.012). Extract of bark (p = 0.075) or leaf (p = 0.005) associated with ACA antibiotic showed antimicrobial activity against gram-positive bacteria. Our study suggests that the bark and leaf extracts contain bioactive compounds with antimicrobial activity against multidrug resistant strains.
a Universidade Federal do Tocantins , Palmas-Tocantins , Brazil.
Joelma da Costa
JDC
Borges
a Universidade Federal do Tocantins , Palmas-Tocantins , Brazil.
Michele Cezimbra
MC
Perim
b Cell Cycle and Cancer Biology Research Program , Oklahoma Medical Research Foundation , Oklahoma City , OK , USA.
Rodrigo Orlandini
RO
de Castro
a Universidade Federal do Tocantins , Palmas-Tocantins , Brazil.|c Departamento de Ciências Farmacêuticas , Universidade Federal de Pernambuco , Recife , Brazil.
Thiago Antônio de Sousa
TAS
Araújo
c Departamento de Ciências Farmacêuticas , Universidade Federal de Pernambuco , Recife , Brazil.
Tadeu José da Silva
TJDS
Peixoto Sobrinho
d Hospital de Base do Distrito Federal, SMHS , Brasília , Brazil.
Ana Carolina Oliveira
ACO
da Silva
a Universidade Federal do Tocantins , Palmas-Tocantins , Brazil.
Sandra Maria Botelho
SMB
Mariano
a Universidade Federal do Tocantins , Palmas-Tocantins , Brazil.
Solange Cristina
SC
Carreiro
e Departamento de Genética e Evolução , Universidade Federal de São Carlos , São Carlos , Brazil.
Maria Cristina da Silva
MCDS
Pranchevicius
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001938:Brazil; D018432:Drug Resistance, Multiple / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D029586:Moraceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
D000900:Anti-Bacterial Agents; D010936:Plant Extracts
Multidrug resistant bacteria; antimicrobial activity; diabetic foot infection; pharmacological potential, medicinal plants