Phenolic Fractions from Muscadine Grape "Noble" Pomace can Inhibit Breast Cancer Cell MDA-MB-231 Better than those from European Grape "Cabernet Sauvignon" and Induce S-Phase Arrest and Apoptosis.
Tons of grape pomace which still contained a rich amount of plant polyphenols, is discarded after winemaking. Plant polyphenols have multi-functional activities for human body. In this study, polyphenols of pomaces from Muscadinia rotundifolia "Noble" and Vitis vinifera "Cabernet Sauvignon" were extracted and fractionated, and then they were analyzed with LC-MS and the inhibitory effects on breast cancer cells were compared. The inhibition on MDA-MB-231 cells of fractions from "Noble" was further evaluated. The results showed that polyphenols from 2 grape pomaces could be separated into 3 fractions, and ellagic acid and/or ellagitannins were only detected in fractions from "Noble" pomace. All 3 fractions from "Noble" pomace inhibited MDA-MB-231 better than MCF-7. But fraction 2 from "Cabernet Sauvignon" inhibited MCF-7 better while fraction 1 and fraction 3 inhibited both 2 cells similarly. Moreover, the fractions from "Noble" pomace rather than "Cabernet Sauvignon" can inhibit MDA-MB-231 better. Finally, fractions from "Noble" pomace can induce S-phase arrest and apoptosis on MDA-MB-231. These findings suggested the extracts from grape pomace especially those from "Noble," are potential to be utilized as health beneficial products or even anti-breast cancer agents.
The Viticulture and Enology Program, College of Food Science and Nutritional Engineering, China Agricultural Univ., Beijing, China.|Dept. of Food Science and Engineering, Jinan Univ., Guangzhou, China.
Jianming
J
Luo
The Viticulture and Enology Program, College of Food Science and Nutritional Engineering, China Agricultural Univ., Beijing, China.|Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Guangxi Academy of Agricultural Science, Guangxi, China.
Zheng
Z
Wei
The Viticulture and Enology Program, College of Food Science and Nutritional Engineering, China Agricultural Univ., Beijing, China.
Shengyu
S
Zhang
Dept. of Food Science and Engineering, Jinan Univ., Guangzhou, China.
Xichun
X
Peng
Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Guangxi Academy of Agricultural Science, Guangxi, China.
Yu
Y
Huang
The Viticulture and Enology Program, College of Food Science and Nutritional Engineering, China Agricultural Univ., Beijing, China.
Yali
Y
Zhang
Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Guangxi Academy of Agricultural Science, Guangxi, China.|Center for Viticulture and Enology, School of Agriculture and Biology, Shanghai Jiao Tong Univ., Shanghai, China.
Jiang
J
Lu
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D005260:Female; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols; D010936:Plant Extracts
apoptosis; breast cancer cell; cell cycle; ellagic acid; muscadine grape pomace