c-Fos activity in the insular cortex, nucleus accumbens and basolateral amygdala following the intraperitoneal injection of saccharin and lithium chloride.
This study examined c-Fos expression in selected brain areas consequent to intraperitoneal (IP) administration of saccharin and lithium chloride. Rats were tested for aversion to the saccharin as measured by flavor consumption and orofacial reactions in the taste reactivity (TR) test. It was found that intraperitoneal conditioning resulted in the reduction in voluntary consumption but not in the production of aversive orofacial responses to the saccharin. The immunohistochemistry quantification revealed increased c-Fos activity in the insular cortex, the shell and core regions of the nucleus accumbens, and the basolateral nucleus of the amygdala. These results show that a conditioned taste aversion can be induced without direct oropharyngeal gustatory stimulation at the time of conditioning. In addition, this study provide evidence of increased neural activity in response to intraperitoneal saccharin injections.
Department of Psychology, University of Oviedo, Oviedo, Spain.
Alberto
A
Soto
Department of Psychology, University of Oviedo, Oviedo, Spain.
Patricia
P
Gasalla
Department of Psychology, University of Oviedo, Oviedo, Spain.
Azucena
A
Begega
Department of Psychology, University of Oviedo, Oviedo, Spain. Electronic address: mlopez@uniovi.es.
Matías
M
López
D000818:Animals; D001362:Avoidance Learning; D066272:Basolateral Nuclear Complex / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D018021:Lithium Chloride / Q000008:administration & dosage*; D008297:Male; D009714:Nucleus Accumbens / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D017208:Rats, Wistar; D012439:Saccharin / Q000008:administration & dosage*; D013649:Taste
D016760:Proto-Oncogene Proteins c-fos; D012439:Saccharin; D018021:Lithium Chloride
Injected flavor; Rats; Taste aversion; Taste reactivity; c-Fos activity