The role of miR-2∼13∼71 cluster in resistance to deltamethrin in Culex pipiens pallens.
Excessive and continuous application of deltamethrin has resulted in the development of deltamethrin resistance among mosquitoes, which becomes a major obstacle for mosquito control. In a previous study, differentially expressed miRNAs between deltamethrin-susceptible (DS) strain and deltamethrin-resistant (DR) strain using illumina sequencing in Culex pipiens pallens were identified. In this study, we applied RNAi and the Centers for Disease Control and Prevention (CDC) bottle bioassay to investigate the relationship between miR-2∼13∼71 cluster (miR-2, miR-13 and miR-71) and deltamethrin resistance. We used quantitative real-time PCR (qRT-PCR) to measure expression levels of miR-2∼13∼71 clusters. MiR-2∼13∼71 cluster was down regulated in adult female mosquitoes from the DR strain and played important roles in deltamethrin resistance through regulating target genes, CYP9J35 and CYP325BG3. Knocking down CYP9J35 and CYP325BG3 resulted in decreased mortality of DR mosquitoes. This study provides the first evidence that miRNA clusters are associated with deltamethrin resistance in mosquitoes. Moreover, we investigated the regulatory networks formed between miR-2∼13∼71 cluster and its target genes, which provide a better understanding of the mechanism involved in deltamethrin resistance.
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Qin
Q
Guo
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Yun
Y
Huang
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China; Microbiology and Immunology Department, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, PR China.
Feifei
F
Zou
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China; Department of Clinical Laboratory, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu, 225001, PR China.
Bingqian
B
Liu
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Mengmeng
M
Tian
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Wenyun
W
Ye
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Juxin
J
Guo
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Xueli
X
Sun
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Dan
D
Zhou
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Yan
Y
Sun
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China.
Lei
L
Ma
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China. Electronic address: shenbo@njmu.edu.cn.
Bo
B
Shen
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, PR China. Electronic address: clzhu@njmu.edu.cn.
Changliang
C
Zhu
D000818:Animals; D003465:Culex / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides; D035683:MicroRNAs / Q000378:metabolism*; D009570:Nitriles; D011722:Pyrethrins
D019476:Insect Proteins; D007306:Insecticides; D035683:MicroRNAs; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System
CYP325BG3; CYP9J35; Culex pipiens pallens; Deltamethrin resistance; miR-2∼13∼71 cluster