From rabbit antibody repertoires to rabbit monoclonal antibodies.
In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.
Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.
Justus
J
Weber
Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.
Haiyong
H
Peng
Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.
Christoph
C
Rader
D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques / Q000379:methods; D011817:Rabbits / Q000276:immunology*
D061067:Antibodies, Monoclonal, Humanized