Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA.
Here we described the preparation and characterization by atomic force microscopy of dumbbell-shaped conjugates between 450 bp double-stranded DNA polymer, poly(dG)-poly(dC), and 5 nm gold nanoparticles (GNPs). We have demonstrated that the size of the nanoparticles in the conjugates can be increased in a controlled fashion. Application of the conjugates for measuring the electrical conductivity of DNA is discussed.
Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel. g.eidelshtein@gmail.com.
Gennady
G
Eidelshtein
Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel. moranfattal@gmail.com.
Moran
M
Fattal
Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel. gabihem@walla.co.il.
Gavriel
G
Avishai
Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel. kempinski@mail.tau.ac.il.
Benjamin
B
Kempinski
Department of Chemistry, University of Milan, via Golgi 19, 20133 Milan, Italy. clelia.giannini@unimi.it.
Clelia
C
Giannini
Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel. s2shak@post.tau.ac.il.
Alexander
A
Kotlyar
DNA nanotechnology; atomic force microscopy; gold nanoparticles; poly(dG)-poly(dC)