Cytotoxic Steroids from the Vietnamese Soft Coral Sinularia leptoclados.
Fifteen steroids, including two new compounds, leptosteroid (1) and 5,6β-epoxygorgosterol (2), were isolated and structurally elucidated from the Vietnamese soft coral Sinularia leptoclados. Their cytotoxic effect against a panel of eight human cancer cell lines was evaluated using sulforhodamine B (SRB) method. Significant cytotoxicity against hepatoma cancer (HepG2, IC50=21.13±0.70 µM) and colon adenocarcinoma (SW480, IC50=28.65±1.53 µM) cell lines were observed for 1 and against acute leukemia (HL-60, IC50=20.53±2.26 µM) and SW480 (IC50=26.61±1.59 µM) for ergost-5-en-3β,7β-diol (8). In addition, 3β,7β-dihydroxyergosta-5,24(28)-diene (13) showed significant cytotoxic activity on all tested cell lines with IC50values ranging from 13.45±1.81 to 29.01±3.21 µM.
Advanced Center for Bioorganic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).|Graduate University of Science and Technology, VAST.
Ninh Thi
NT
Ngoc
Advanced Center for Bioorganic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).
Tran Thi Hong
TTH
Hanh
Advanced Center for Bioorganic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).
Nguyen Van
NV
Thanh
Institute of Biotechnology, VAST.
Do Thi
DT
Thao
Advanced Center for Bioorganic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).|Graduate University of Science and Technology, VAST.
Nguyen Xuan
NX
Cuong
Advanced Center for Bioorganic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).|Graduate University of Science and Technology, VAST.
Nguyen Hoai
NH
Nam
Institute of Marine Environment and Resources, VAST.
Do Cong
DC
Thung
Advanced Center for Bioorganic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).
Phan Van
PV
Kiem
Advanced Center for Bioorganic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).
Chau Van
CV
Minh
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D013256:Steroids / Q000032:analysis*; D014744:Vietnam
D000970:Antineoplastic Agents; D013256:Steroids
Alcyoniidae; Sinularia leptoclados; cytotoxic activity; soft coral; steroid