CDK inhibitor SU9516 induces tetraploid blastocyst formation from parthenogenetically activated porcine embryos.
OBJECTIVE
To examine the effect of SU9516, a cyclin-dependent kinase inhibitor, on the induction of tetraploid blastocyst formation in porcine embryos by parthenogenetic activation.


RESULTS
Karyotype analysis of blastocysts showed that in the SU9516-treatment group 56% were tetraploid, whereas in the cytochalasin B (CB) group 67% were diploid. The level of maturation-promoting factor (MPF) in stimulated embryos treated with 10 µM SU9516 for 4 h was lower than in embryos treated with CB group (103 vs. 131 pg/ml). The mRNA expression levels of Nanog significantly increased in SU9516-treated embryos than CB group.


CONCLUSIONS
SU9516 can induce tetraploid blastocyst formation at high efficiency. SU9516 can significantly influence the in vitro developmental competence of porcine parthenogenetically activated embryos by influencing the level of MPF and the gene related apoptosis and pluripotency.
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Qing
Q
Guo
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Long
L
Jin
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Hai-Ying
HY
Zhu
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Xiao-Chen
XC
Li
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Yu-Chen
YC
Zhang
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Xiao-Xu
XX
Xing
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Guang-Lei
GL
Zhang
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Mei-Fu
MF
Xuan
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Qi-Rong
QR
Luo
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Zhao-Bo
ZB
Luo
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Jun-Xia
JX
Wang
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China.
Jin-Dan
JD
Kang
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133002, Jilin, China. yinxj33@msn.com.
Xi-Jun
XJ
Yin
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D003571:Cytochalasin B / Q000378:metabolism; D007093:Imidazoles / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D007621:Karyotyping; D047428:Protein Kinase Inhibitors / Q000378:metabolism*; D013552:Swine / Q000196:embryology; D057891:Tetraploidy
D007093:Imidazoles; D007211:Indoles; D047428:Protein Kinase Inhibitors; C433985:SU 9516; D003571:Cytochalasin B
Embryos; Maturation-promoting factor; Parthenogenetic activation; Pigs; Polar body; SU9516; Tetraploid embryos