Modeling multidimensional and multispecies biofilms in porous media.
Modeling multidimensional and multispecies biofilm in porous media at the pore scale is challenging due to the need to simultaneously track the microbial community in the biofilms and the interfaces between the biofilms and the fluid. Therefore, researchers usually assume that the model has only one dimension in space or has only one microbial species. This work uses bioremediation of U(VI)-contaminated groundwater as the context to develop a two-dimensional and multispecies biofilm model. The model simulates the transverse mixing zone in which U(VI) is mixed with propionate, a nutrient externally supplied to stimulate the growth of microorganisms. The model considers multiple interactions among fluid flow, transport and reaction of chemical species, and growth of biofilm. The biofilm consists of two types of active biomass (syntrophs and dissimilatory metal reducing bacteria [DMBR]) and inert biomass. The two types of active biomass collaboratively remove U(VI). The model outputs biomass distribution, chemical species concentrations, and fluid flow at the pore scale to fundamentally study the multiple interactions. The model also outputs the contaminant removal rate that can be potentially used for up-scaling studies. The simulated results are generally consistent with experimental observations from other studies in trend. The trend can be explained by the multiple interactions based on thermodynamics and microbial kinetics. Biotechnol. Bioeng. 2017;114: 1679-1687. © 2017 Wiley Periodicals, Inc.
Department of Civil and Environmental Engineering, Florida A&M University-Florida State University College of Engineering, Florida State University, Tallahassee, Florida.
Youneng
0000-0001-8494-2908
Y
Tang
School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
Haihu
H
Liu
D018407:Bacterial Physiological Phenomena; D018441:Biofilms / Q000254:growth & development*; D003198:Computer Simulation; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D060587:Groundwater / Q000382:microbiology; D064307:Microbiota / Q000502:physiology*; D008954:Models, Biological; D016062:Porosity; D013045:Species Specificity
D003470:Culture Media
biofilm model; bioremediation; cellular automaton; pore scale; syntroph; uranium