A novel polyaniline/polypyrrole/graphene oxide fiber for the determination of volatile organic compounds in headspace gas of lung cell lines.
Exploration of volatile organic compounds (VOCs) generated by lung cell lines is a powerful and non-invasive tool for the detection of potential volatile biomarkers of lung cancer. In this study, a simple and sensitive solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) method was developed for the determination of VOCs in the headspace gas of lung cell lines. For the purpose of preconcentration, a novel polyaniline/polypyrrole/graphene oxide (PANI/PPy/GO) coating was prepared on the surface of stainless steel fiber via in-situ electrochemical deposition for the first time. The characteristic properties of the coating were studied and the results revealed that the coating possessed large surface area, high extraction efficiency, excellent thermal and mechanical stability as well as long lifespan. Some parameters affecting the extraction efficiency such as synthesis conditions, extraction and desorption conditions were optimized. Under the optimal conditions, the method displayed relatively wide linear range (three or four orders of magnitude) with correlation coefficients above 0.9916. Low detection limits from 1.0 to 12ngL-1were obtained. Relative standard deviations ranged from 1.2% to 18.0% indicating good repeatability and reproducibility of the method. This method has been successfully applied to analyze VOCs in the headspace gas of lung adenocarcinoma epithelial cell line (A549) and human embryonic fibroblast cell line (MRC-5).
Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China.
JingHong
J
Li
Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. Electronic address: huixu@mail.ccnu.edu.cn.
Hui
H
Xu
D000230:Adenocarcinoma / Q000378:metabolism*; D000077192:Adenocarcinoma of Lung; D000814:Aniline Compounds / Q000737:chemistry*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000302:isolation & purification*
D000814:Aniline Compounds; D011108:Polymers; D011758:Pyrroles; D055549:Volatile Organic Compounds; C416807:polyaniline; C067635:polypyrrole; D006108:Graphite
Conducting polymer; Gas chromatography-mass spectrometry; Graphene oxide; Lung cell lines; Solid phase microextraction; Volatile organic compounds