Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells.
We describe a comprehensive method for imaging and analysis of local protein dynamics at single sites of exocytosis in living cultured endocrine cells. This method is well suited to quantitatively map the complex dynamics of individual molecules at single sites of vesicle fusion in live cells.
Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Adam J
AJ
Trexler
Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, Room 3312, 50 South Drive, Bethesda, MD, 20892, USA. justin.taraska@nih.gov.
Justin W
JW
Taraska
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D055098:Endocrine Cells / Q000378:metabolism*; D005089:Exocytosis; D017930:Genes, Reporter; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*
D008164:Luminescent Proteins
Endocrine cells; Exocytosis; Image analysis; Live-cell imaging; TIRF; Vesicle fusion