HPLC-DAD analysis, antinociceptive and anti-inflammatory properties of the ethanolic extract of Hyptis umbrosa in mice.
Hyptis umbrosa(syn.Mesosphaerum sidifolium) (Lamiaceae Family) has been used to treat several conditions such as gastrointestinal disorders, skin infections, nasal congestion, fever and cramps. The objective of this study was to evaluate the chemical composition, analgesic and anti-inflammatory profiles of ethanol extract from leaves ofHyptis umbrosa(EEB). HPLC-DAD was used to determine the fingerprint chromatogram of the extract. Male Swiss mice were orally pretreated with EEB (100, 200 or 400 mg/kg; 60 min before initiating algesic stimulation) and antinociceptive activity was assessed using the acetic acid-induced writhing model, formalin test and hyperalgesia induced by glutamate or capsaicin. Also, peritonitis was induced by the intrathoracic injection of carrageenan to quantify the total number of leukocytes. The presence of phenolic compounds in the extract was confirmed using HPLC-DAD. The treatment with EEB, at all doses, produced a significant analgesic effect against acetic acid-induced antinociceptive activity. In the formalin test, only the 400-mg/kg-dose of EEB had a significant effect in the first phase. However, all doses tested were able to reverse nociception in the second phase. The effect of all doses of EEB also showed a significant antinociceptive effect in the glutamate and capsaicin tests and inhibited the carrageenan-induced leukocyte migration to the peritoneal cavity. The present study suggests that the EEB possesses peripheral analgesic action and showed potential in reducing the spreading of the inflammatory processes. Also, it seems to be related with vanilloid and glutamate receptors.
Department of Physiology. Laboratory of Neurosciences and Pharmacological Assays Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Klécia S
KS
Dos Anjos
Department of Physiology. Laboratory of Neurosciences and Pharmacological Assays Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Heitor G
HG
Araújo-Filho
Department of Physiology. Laboratory of Neurosciences and Pharmacological Assays Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Marcelo C
MC
Duarte
Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
Vicente C O
VC
Costa
Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
Josean F
JF
Tavares
Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
Marcelo S
MS
Silva
Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
Jackson R G S
JR
Almeida
Analytical Center of Drugs, Medicines and Food - CAFMA. Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
Nathália A C
NA
Souza
Analytical Center of Drugs, Medicines and Food - CAFMA. Federal University of San Francisco Valley, Petrolina, Pernambuco, Brazil.
Larissa A
LA
Rolim
Department of Biological Chemistry. Regional University of Cariri, Crato, CE, Brazil.
Irwin R A
IR
Menezes
Department of Biological Chemistry. Regional University of Cariri, Crato, CE, Brazil.
Henrique D M
HD
Coutinho
Department of Physiology. Laboratory of Neurosciences and Pharmacological Assays Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Jullyana S S
JS
Quintans
Department of Physiology. Laboratory of Neurosciences and Pharmacological Assays Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Lucindo J
LJ
Quintans-Júnior
inflammatory pain; medicinal plants; nociception; phenolic compounds