Ischemia deteriorates spike encoding at cortical GABAergic neurons and cerebellar Purkinje cells by increasing the intracellular Ca2.
GABAergic neurons play a critical role in the central nervous system, such as well-organized behaviors. The ischemic cell death is presumably initiated by neuronal excitotoxicity resulted from the dysfunction of GABAergic neurons. It is not clear how ischemia influences different types of GABAergic neurons and whether intracellular Ca2+plays a key role in the ischemic excitotoxicity. We have investigated this issue at cortical GABAergic neurons and cerebellar Purkinje cells by whole-cell recording in mouse brain slices, and the roles of intracellular Ca2+are examined by BABTA infusion. Compare with the data from a group of control, ischemia causes by lowering purfusion rate lowers spike encoding at cortical GABAergic neurons and enhances encoding ability at cerebellar Purkinje cells. These differential effects of ischemia on spike encoding are mechanistically associated with the changes in the refractory periods and threshold potentials of sequential spikes. These ischemia-induced dysfunction of spike encoding at two types of GABAergic cells are prevented by BABTA infusion. Therefore, the ischemia destabilizes the spike encoding of GABAergic cells via raising intracellular Ca2+. Our findings indicate that ischemia preferentially causes the dysfunction of spike encoding at GABAergic neurons by the up-regulation of intracellular Ca2+level, which leads to neuronal excitotoxicity.
Department of Pathophysiology, Bengbu Medical College, Bengbu, Anhui 233030, China.
Li
L
Huang
Department of Endocrinology, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233040, China.
Chun
C
Wang
Department of Pathophysiology, Bengbu Medical College, Bengbu, Anhui 233030, China.
Rongjing
R
Ge
Department of Pathophysiology, Bengbu Medical College, Bengbu, Anhui 233030, China.
Hong
H
Ni
Department of Pathophysiology, Bengbu Medical College, Bengbu, Anhui 233030, China. Electronic address: zi05@163.com.
Shidi
S
Zhao
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000503:physiopathology; D002545:Brain Ischemia / Q000503:physiopathology*; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D059330:GABAergic Neurons / Q000502:physiology*; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D008815:Mice, Inbred Strains; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000378:metabolism / Q000502:physiology*; D015854:Up-Regulation; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005680:gamma-Aminobutyric Acid; D002118:Calcium
Action potential; GABAergic neuron; Intracellular Ca(2+); Ischemia; Purkinje cells; Whole-cell recording