The trans-Golgi Network and the Golgi Stacks Behave Independently During Regeneration After Brefeldin A Treatment in Tobacco BY-2 Cells.
The trans-Golgi network (TGN) plays an essential role in intracellular membrane trafficking. In plant cells, recent live-cell imaging studies have revealed the dynamic behavior of the TGN independent from the Golgi apparatus. In order to better understand the relationships between the two organelles, we examined their dynamic responses to the reagent brefeldin A (BFA) and their recovery after BFA removal. Golgi markers responded to BFA similarly over a range of concentrations, whereas the behavior of the TGN was BFA concentration dependent. The TGN formed aggregates at high concentrations of BFA; however, TGN proteins relocalized to numerous small vesicular structures dispersed throughout the cytoplasm at lower BFA concentrations. During recovery from weak BFA treatment, the TGN started to regenerate earlier than the completion of the Golgi. The regeneration of the two organelles proceeded independently of each other for a while, and eventually was completed by their association. Our data suggest that there is some degree of autonomy for the regeneration of the TGN and the Golgi in tobacco BY-2 cells.
Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama, Japan.
Yoko
Y
Ito
Mass Spectrometry and Microscopy Unit, Technology Platform Division, RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa, Japan.
Kiminori
K
Toyooka
Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Masaru
M
Fujimoto
Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi, Japan.|Department of Basic Biology, Graduate University for Advanced Studies, Okazaki, Aichi, Japan.|Japan Science and Technology Agency (JST), PRESTO, Kawaguchi, Saitama, Japan.
Takashi
T
Ueda
Laboratory of Developmental Cell Biology, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Tomohiro
T
Uemura
Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama, Japan.|Laboratory of Developmental Cell Biology, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Akihiko
A
Nakano
D020126:Brefeldin A / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D059828:Plant Cells / Q000187:drug effects; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011726:Pyridinium Compounds / Q000493:pharmacokinetics; D000644:Quaternary Ammonium Compounds / Q000493:pharmacokinetics; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects*; D021601:trans-Golgi Network / Q000187:drug effects / Q000378:metabolism*
C092350:FM 4-64; D005456:Fluorescent Dyes; D010940:Plant Proteins; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D020126:Brefeldin A
BFA; BY-2 cells; Golgi; TGN