Prenatal noise and restraint stress interact to alter exploratory behavior and balance in juvenile rats, and mixed stress reverses these effects.
We aimed to investigate in adolescent rats the individual and combined effects of prenatal noise and restraint stress on balance control, exploration, locomotion and anxiety behavior. Three groups of pregnant rats were exposed to daily repeated stress from day 11 to day 19 of pregnancy: 3 min noise (Noise Stress, NS); 10 min restraint (restraint stress, RS); or 3 min noise followed by 10 min restraint (mixed stress, MS). On postnatal days (PND) 44, 45 and 46, four groups of male rats (Control, NS, RS:, MS; 16 rats each), were tested as follows: (1) beam walking (BW), (2) open field (OF) and (3) elevated plus maze (EPM). Our results show that the NS group had significantly impaired balance control, locomotion and both horizontal and vertical exploration (p < .01 for all measures). The RS group showed only a decrease in vertical exploration (p < .05). In contrast, locomotion and balance were not affected in the MS group (OF: crossed squares: p = .34, missteps: p = .18). However, MS rats exhibited significantly higher anxiety levels (less time in EPM open arms: p < .05), and took more time to complete BW: p < .05). Hence, combined prenatal stressors exert non-additive effects on locomotion, exploration and balance control, but induce greater anxiety through additive effects. Terminal plasma ACTH concentration was increased by prenatal stress, especially noise, which group had the largest adrenal glands. Overall, contrary to expectation, combined prenatal stressors can interact to increase anxiety level, but diminish alteration of exploration, locomotion and impaired balance control, which were strongly induced by noise stress. Lay summary: Experience of stress in pregnancy can have negative effects on the offspring that are long-lasting. Here, we used laboratory rats to see whether repeated episodes of exposure to loud noise or preventing free movement, alone or together, during pregnancy had different effects on behaviors of the adolescent offspring. Using standard tests, we found the prenatal stresses caused the offspring to be anxious, and not to balance when moving around as well as normal offspring; the degree of impairment depended on the type of stress - loud noise exposure had the greatest effects, but if the stresses were combined the effects were not worse. The results point to the need to aim to avoid stress in pregnant women.
a Laboratory of Biochemistry and Applied Microbiology, Department of Biochemistry, Faculty of Science , University Badji Mokhtar-Annaba , Algeria.
Soumeya
0000-0002-0361-5735
S
Badache
b Department of Biochemistry, Faculty of Science , University Badji Mokhtar-Annaba , Algeria.
Slim
0000-0002-4765-1463
S
Bouslama
c Department of Medicine, Faculty of Medical Sciences , University Badji Mokhtar-Annaba , Algeria.
Oualid
0000-0002-7457-6569
O
Brahmia
d Laboratory of Applied Neuroendocrinology, Department of Biology, Faculty of Science , University Badji Mokhtar-Annaba , Algeria.
Abdel Madjid
0000-0003-1935-5648
AM
Baïri
d Laboratory of Applied Neuroendocrinology, Department of Biology, Faculty of Science , University Badji Mokhtar-Annaba , Algeria.
Abdel Krim
0000-0001-7114-9556
AK
Tahraoui
a Laboratory of Biochemistry and Applied Microbiology, Department of Biochemistry, Faculty of Science , University Badji Mokhtar-Annaba , Algeria.
Ali
0000-0002-7540-366X
A
Ladjama
D000818:Animals; D001007:Anxiety / Q000523:psychology*; D001522:Behavior, Animal; D005106:Exploratory Behavior; D005260:Female; D008124:Locomotion; D008297:Male; D009622:Noise; D004856:Postural Balance; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000523:psychology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000523:psychology*; D013997:Time Factors
Anxiety; balance; beam walking; locomotion; noise; prenatal stress