Substance P expression in the gingival tissue after upper third molar extraction: effect of ketoprofen, a preliminary study.
The aim of this study was to evaluate substance P (SP) levels and the effect of a non-steroidal anti-inflammatory drug (NSAID), ketoprofen, on SP in the pericoronal gingival tissue after extraction of upper third molars. A sample of 20 young non-smoking systemically healthy adults of both sexes, with a healthy upper third molar to extract for orthodontic purposes, was selected. After extraction, a sample of the gingival tissue of the pericoronal region was collected with a sterile scalpel, placed into test tubes and kept frozen at -20°C until the SP determination. SP levels were determined by using a commercially available enzyme immunoassay (ELISA) kit. The subjects were randomly divided into two groups: group 1 received a single dose of ketoprofen 30 minutes prior to the experimental procedure. The subjects of group 2 did not receive any kind of drug administration before extraction. The patients were asked to complete a diary on the postoperative pain. A relevant amount of SP was measured in all the gingival samples. No statistically significant difference could be detected in SP expression between the two groups. In group 1 pain appearance was significantly delayed (6.2±0.13 hours) in comparison with group 2 (3.95±0.2 hours). In this small selected group of subjects and limited study design, preventive administration of ketoprofen did not significantly affect the gingival levels of SP, the clinical recommendation emerging is that of NSAID administration postoperatively but before pain appearance in order to optimize the management of pain of the patient.
Faculty of Dentistry and Oral Hygiene, University of Insubria, Varese, Italy.
G M
GM
Abbate
Faculty of Dentistry and Oral Hygiene, University of Insubria, Varese, Italy.
A
A
Mangano
Dipartimento di Scienze Farmacologiche e Biomolecolari, University of Milan, Milano, Italy.
P
P
Sacerdote
Dipartimento di Scienze Farmacologiche e Biomolecolari, University of Milan, Milano, Italy.
G
G
Amodeo
Dipartimento di Scienze Farmacologiche e Biomolecolari, University of Milan, Milano, Italy.
G
G
Moschetti
Faculty of Dentistry and Oral Hygiene, University of Insubria, Varese, Italy.
L
L
Levrini
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D005881:Gingiva / Q000187:drug effects / Q000294:innervation / Q000601:surgery; D006801:Humans; D007660:Ketoprofen / Q000627:therapeutic use*; D008297:Male; D008964:Molar, Third / Q000601:surgery*; D010147:Pain Measurement / Q000379:methods*; D010149:Postoperative Pain / Q000503:physiopathology / Q000517:prevention & control*; D013373:Substance P / Q000235:genetics* / Q000378:metabolism; D014081:Tooth Extraction
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013373:Substance P; D007660:Ketoprofen