Comparison of commercial methods of immunoblot, ELISA, and chemiluminescent immunoassay for detecting type-specific herpes simplex viruses-1 and -2 IgG.
BACKGROUND
Serology for type-specific herpes simplex virus (HSV) is based on the use of the respective glycoprotein G (gG).


METHODS
Chemiluminescent immunoassay (CLIA; BIO-FLASH®, Biokit, Spain), ELISA (HerpeSelect®, Focus, USA), and immunoblot (IB; Virotech, Germany) for detecting HSV-1- and HSV-2-specific IgG were compared using 384 serum samples received for HSV serology. The samples were classified as positive or negative according to a consensus criterion.


RESULTS
For HSV-1, 262 samples were positive and 118 were negative (four samples were unclassifiable). IB showed agreement, sensitivity, and specificity values of 98.68%, 98.47% and 99.15%, respectively. The corresponding figures for CLIA and ELISA were 98.95%, 99.24% and 98.31%, and 98.16%, 99.62% and 94.92%, respectively. For HSV-2, 106 samples were positive and 278 were negative. Agreement, sensitivity, and specificity of IB were 99.48%, 98.11%, and 100%, respectively. The corresponding figures for CLIA and ELISA were 99.48%, 99.06% and 99.64%, and 98.18%, 99.06% and 97.84%, respectively.


CONCLUSIONS
The three methods showed excellent and equivalent performance characteristics for the detection of type-specific IgG to HSV-1 and HSV-2.
Laboratorio de Serología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.|CIBER Epidemiology and Public Health, Madrid, Spain.
Fernando
http://orcid.org/0000-0002-6981-1261
F
de Ory
Laboratorio de Serología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.|CIBER Epidemiology and Public Health, Madrid, Spain.
María-Eulalia
ME
Guisasola
Laboratorio de Serología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.
Pilar
P
Balfagón
CIBER Epidemiology and Public Health, Madrid, Spain.|Laboratorio Regional de Salud Pública, Comunidad de Madrid, Madrid, Spain.
Juan Carlos
JC
Sanz
D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006561:Herpes Simplex; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D007074:Immunoglobulin G
chemiluminescent immunoassay; enzyme immunoassay; herpes simplex virus type 1; herpes simplex virus type 2; immunoblot