Immunophenotyping of blood mononuclear cells fromP.berghei(NK-65) infected and immunized BALB/c mice.
The present study demonstrates the alterations in the frequencies of helper, cytotoxic and suppressor T cells in blood ofP.bergheiinfected and TPA immunized rodent host towards the induction of protective immune response. Statistically significant (p< 0.005) number of CD4+T cells was recorded on D9 [Post Challenge (PC)] meanwhile CD4+Treg cells were found to be declined in immunized mice as compared to infected. This increase in frequencies of T helper cells points towards the stimulation of strong Th2 immune response in immunized mice. Statistically significant (p< 0.005) decline was observed in the frequencies of CD8+T cells on D9 (PC) in immunized mice. In infected controls cytotoxic T cells were also found to be increased after 24 h post infection. Due to strong Th2 immune response, evident from lower frequencies of CD4+Treg cells in immunized mice, complete protection was obtained. T reg population was found to be higher in infected controls and all control mice died.
Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014 India.
Vineet
V
Kumar
Department of Translational and Regenerative Medicine, PGIMER, Chandigarh, 160012 India.
Aruna
A
Rakha
Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014 India.
Ruchika
R
Saroa
Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014 India.
Upma
U
Bagai
Plasmodium berghei; T cytotoxic; T helper; T suppressor cells