Serum Wisteria Floribunda Agglutinin-Positive Sialylated Mucin 1 as a Marker of Progenitor/Biliary Features in Hepatocellular Carcinoma.
Histological molecular classification of hepatocellular carcinoma (HCC) is clinically important for predicting the prognosis. However, a reliable serum marker has not been established. The aim of this study was to evaluate the diagnostic value of serum Wisteria Floribunda agglutinin-positive sialylated mucin 1 (WFA-sialylated MUC1), which is a novel biliary marker, as a marker of HCC with hepatic progenitor cell (HPC)/biliary features and of prognosis. A total of 144 consecutive patients who underwent complete radiofrequency ablation of primary HCC were enrolled. A serum WFA-sialylated MUC1 level of 900 μL/mL was determined as the optimal cutoff value for prediction of immunohistochemical staining for HPC/biliary features [sialylated MUC1 and cytokeratin 19 (CK19)]. Positive staining rate of sialylated MUC1 and CK19 was significantly higher in patients with WFA-sialylated MUC1 ≥900 than those with WFA-sialylated MUC1 <900. Furthermore, cumulative incidence of HCC recurrence was significantly higher in patients with WFA-sialylated MUC1 ≥900 and on multivariate analysis, serum WFA-sialylated MUC1 levels was an independent predictor of HCC recurrence. These results revealed that serum WFA-sialylated MUC1 was associated with histological feature of HCC and recurrence after curative therapy and it could be a novel marker of HPC/biliary features in HCC and of prognosis.
Department of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Tokyo, Japan.
Nobuharu
N
Tamaki
Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan.
Atsushi
A
Kuno
Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan.
Atsushi
A
Matsuda
Department of Pathology, Keio University School of medicine, Tokyo, Japan.
Hanako
H
Tsujikawa
Department of Pathology, Keio University School of medicine, Tokyo, Japan.
Ken
K
Yamazaki
Department of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Tokyo, Japan.
Yutaka
Y
Yasui
Department of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Tokyo, Japan.
Kaoru
K
Tsuchiya
Department of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Tokyo, Japan.
Hiroyuki
H
Nakanishi
Department of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Tokyo, Japan.
Jun
J
Itakura
Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Chiba, Japan.
Masaaki
M
Korenaga
Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Chiba, Japan.
Masashi
M
Mizokami
Department of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Tokyo, Japan.
Masayuki
M
Kurosaki
Department of Pathology, Keio University School of medicine, Tokyo, Japan.
Michiie
M
Sakamoto
Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan.
Hisashi
H
Narimatsu
Department of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Tokyo, Japan. izumi012@musashino.jrc.or.jp.
Namiki
N
Izumi
D000368:Aged; D015415:Biomarkers / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000473:pathology* / Q000628:therapy; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D053539:Keratin-19 / Q000032:analysis; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000097:blood*; D037121:Plant Lectins / Q000097:blood*; D011379:Prognosis; D034781:Receptors, N-Acetylglucosamine / Q000097:blood*; D012008:Recurrence; D044967:Serum / Q000737:chemistry*
D015415:Biomarkers; D053539:Keratin-19; D018396:Mucin-1; D037121:Plant Lectins; D034781:Receptors, N-Acetylglucosamine; C040129:wisteria lectin