Effect of a novel quaternary ammonium silane cavity disinfectant on durability of resin-dentine bond.
OBJECTIVE
The present study examined the effect of a quaternary ammonium silane (QAS) cavity disinfectant on the viability of human dental pulp cells, dentine bond durability and nanoleakage of simplified etch-and-rinse adhesives.


METHODS
Etched dentine surface of third molars were randomly divided into two adhesive groups, Adper™ Single Bond 2 and Prime & Bond®NT™. For each adhesive, the teeth were randomly assigned to five cavity disinfectant groups (N=6): Group 1: deionised water (control); Group 2: 2% chlorhexidine (CHX); Group 3: 2% QAS; Group 4: 5% QAS and Group 5: 10% QAS. The cavity disinfectants were applied on etched dentine surfaces for 20s, followed by adhesive application. The bonded teeth were sectioned for bond strength testing at 24h, 6 months and 12 months. Viability of human dental pulpal cells was examined using MTT assay. Bond strength data were analysed using 3-way ANOVA and Tukey test. Interfacial nanoleakage was evaluated after 24h and 12 months and analysed using Kruskal-Wallis test.


RESULTS
Significant differences in bond strength were observed for the factors disinfectants (p<0.001) and time (p<0.001); while the factor, adhesive, was not significantly different (p=0.203). The 2% QAS cavity disinfectant preserved bond strength of both adhesives and reduced interfacial nanoleakage after 12 months. Cell viability was the lowest for 2% CHX, followed by 2% QAS and the control.


CONCLUSIONS
The 2% QAS cavity disinfectant demonstrated greater cell viability compared to 2% CHX, with no adverse effect on immediate bond strength and preserved bond stability over time.


CONCLUSIONS
Incorporation of 2% quaternary ammonium silane cavity disinfectant in the resin-dentine bonding protocol enhances the success rate of bonded restorations.
Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong Special Administrative Region.
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D
Daood
Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong Special Administrative Region. Electronic address: ckyyiu@hkucc.hku.hk.
C K Y
CKY
Yiu
Melbourne Dental School, The University of Melbourne, Australia.
M F
MF
Burrow
State Key Laboratory of Military Stomatology, Department of Prosthodontics, Fourth Military Medical University, Xi'an, Shaanxi, PR China.
L-N
LN
Niu
Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
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FR
Tay
D000134:Acid Etching, Dental / Q000379:methods; D000328:Adult; D064751:Ammonium Compounds / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002710:Chlorhexidine / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003731:Dental Caries; D003738:Dental Cements / Q000737:chemistry; D003763:Dental Leakage / Q000145:classification; D003764:Dental Materials / Q000737:chemistry; D003782:Dental Pulp; D003799:Dental Stress Analysis; D003804:Dentin / Q000737:chemistry* / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004202:Disinfectants / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D020782:Matrix Metalloproteinases; D008964:Molar, Third; D010539:Permeability; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012821:Silanes / Q000494:pharmacology*; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D055815:Young Adult
C554641:Adper single bond 2; D064751:Ammonium Compounds; D003738:Dental Cements; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D004202:Disinfectants; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; D012117:Resins, Synthetic; D012821:Silanes; D020782:Matrix Metalloproteinases; D002710:Chlorhexidine
Cysteine cathepsins; Dentine; Matrix metalloproteinases; Quaternary ammonium silane