Potent immune responses induced by a Salmonella ghost delivery system that expresses the recombinant Stx2eB, FedF, and FedA proteins of the Escherichia coli-producing F18 and Shiga toxin in a murine model and evaluation of its protective effect as a porcine vaccine candidate.
BACKGROUND
In the pathogenicity of porcine edema disease (ED), which is caused by the Escherichia coli-producing F18 and Shiga toxin, F18+fimbrial adhesins and Shiga toxin 2e (Stx2e) play pivotal roles in the colonization and enterotoxicity of this pathogen.


OBJECTIVE
To develop a vaccine candidate against ED by combining three selected antigens of F18+E. coli.


METHODS
Genetically engineered Salmonella Typhimurium (ST) ghosts that express Stx2eB, FedF, and FedA were individually inserted in a ghost plasmid cassette, and the resultant plasmids were transformed into an attenuated ST (JOL912). The individual expression of Stx2eB, FedF, and FedA in JOL912 was validated by using an immunoblotting assay.


RESULTS
Immunization of the ghosts in BALB/c mice led to a significant increase in antigen-specific secretory IgA and serum IgG. Significantly marked elevation of the CD3+CD4+T cell subpopulation and lymphocyte proliferating activity in the primed splenocytes were also observed. Furthermore, mRNA of IL-4 and IFN-γ were highly upregulated in in vitro stimulated splenic T cells. Subsequently, the immunized mice showed significant protection efficacy against a lethal dose 50 of a virulent strain, resulting in approximately 85% and 92% survival rates in mice with a single- and double-dose immunization, respectively, compared to only 40% of the non-immunized controls.


CONCLUSIONS
A mixture of the ghosts expressing these three antigens is a potential vaccine candidate for protection against the porcine edema disease.
a College of Veterinary Medicine , Chonbuk National University , Iksan , Republic of Korea.
Gayeon
G
Won
a College of Veterinary Medicine , Chonbuk National University , Iksan , Republic of Korea.
Lee
L
John Hwa
D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D004488:Edema Disease of Swine / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000276:immunology*; D005260:Female; D036781:Fimbriae Proteins / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D012475:Salmonella / Q000276:immunology; D022641:Shiga Toxin 2 / Q000276:immunology*; D013552:Swine; D014614:Vaccines, Synthetic / Q000276:immunology
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; C113053:FedA protein, E coli; C440653:FedF protein, E coli; D022641:Shiga Toxin 2; D014614:Vaccines, Synthetic; D036781:Fimbriae Proteins
F18+ fimbrial adhesion; Porcine edema disease; Shiga toxin 2e; bacterial ghost; murine model; vaccine