Curcuma longa Linn. extract and curcumin protect CYP 2E1 enzymatic activity against mercuric chloride-induced hepatotoxicity and oxidative stress: A protective approach.
The present investigation has been conducted to evaluate the therapeutic potential of Curcuma longa (200mgkg-1, po) and curcumin (80mgkg-1, po) for their hepatoprotective efficacy against mercuric chloride (HgCl2: 12μmolkg-1, ip; once only) hepatotoxicity. The HgCl2administration altered various biochemical parameters, including transaminases, alkaline phosphatase, lactate dehydrogenase, bilirubin, gamma-glutamyl transferase, triglycerides and cholesterol contents with a concomitant decline in protein and albumin concentration in serum which were restored towards control by therapy of Curcuma longa or curcumin. On the other hand, both treatments showed a protective effect on drug metabolizing enzymes viz. aniline hydroxylase (AH) and amidopyrine-N-demethylase (AND), hexobarbitone induced sleep time and BSP retention. Choleretic, 1,1-diphenyl-2-picryl-hydrazil (DPPH)-free radical scavenging activities and histological studies also supported the biochemical findings. The present study concludes that Curcuma longa extract or curcumin has the ability to alleviate the hepatotoxic effects caused by HgCl2in rats.
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research & School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, India; Department of Zoology, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh, India. Electronic address: deepmalajoshi953@gmail.com.
Deepmala
D
Joshi
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research & School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, India.
Deepak Kumar
DK
Mittal
Reproductive Biology and Toxicology Laboratory, UNESCO Satellite Center of Trace Element Research & School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, India.
Sangeeta
S
Shukla
Department of Zoology, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh, India.
Sunil Kumar
SK
Srivastav
School of Pharmacy and Technology Management (SPTM), Shri Vile Parle Kelavani Mandal's (SVKM's),Narsee Monjee Institute of Management Studies (NMIMS), Mukesh Patel Technology Park, Babulde, Bank of Tapi River, Mumbai-Agra Road, Shirpur, Dist. Dhule-India.
Vaibhav A
VA
Dixit
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury; D030024:Curcuma; D003474:Curcumin / Q000494:pharmacology; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects*; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D010936:Plant Extracts; D008627:Mercuric Chloride; C012596:turmeric extract; D019392:Cytochrome P-450 CYP2E1; D003474:Curcumin
Curcuma longa; Curcumin; Hepatoprotection; Histopathology; Mercury toxicity; Oxidative stress