Genetic Polymorphisms in DEFB1 and miRNA202 Are Involved in Salivary Human β-Defensin 1 Levels and Caries Experience in Children.
The antimicrobial peptides human β-defensins (hBDs) are encoded by β-defensin genes (DEFBs) and are possibly involved in caries susceptibility. In this study we aimed (1) to investigate the relationship between salivary hBDs and caries and (2) to evaluate the association of genetic polymorphisms in DEFB1 and microRNA202 (miRNA202) with salivary levels of hBDs and caries experience. Two data sets were available for this study, totalizing 678 Brazilian children. Dental examination and saliva collection were performed in all included children. The salivary level for hDB1, hBD2, and hBD4 was assessed by ELISA sandwich technique in 168 children. The DNA was extracted from saliva, and polymorphisms in DEFB1 and miRNA202 were analyzed by real-time PCR. Statistical analysis was performed to investigate the associations between caries experience, hBD salivary level, genotype, and allele distribution, with an alpha of 0.05. The hBD1 level was significantly higher in caries-free children (p < 0.0001). The miRNA202 was associated with a lower level of salivary hBD1 (p < 0.05). Also, the polymorphic distribution of miRNA202 was associated with caries (p = 0.006). The polymorphisms in DEFB1 were not associated with hBD salivary level and caries experience (p > 0.05). In conclusion, our results indicate that genetic polymorphism in miRNA202 is involved in hBD1 salivary level as well as caries experience in children.
Clinical Research Unit, Fluminense Federal University, Niterói, Brazil.
Andrea
A
Lips
Leonardo Santos
LS
Antunes
Lívia Azeredo
LA
Antunes
Júlia Guimarães Barcellos de
JGB
Abreu
Driely
D
Barreiros
Daniela Silva Barroso de
DSB
Oliveira
Ana Carolina
AC
Batista
Paulo
P
Nelson-Filho
Léa Assed Bezerra da
LABD
Silva
Raquel Assed Bezerra da
RABD
Silva
Gutemberg Gomes
GG
Alves
Erika Calvano
EC
Küchler
D000483:Alleles; D001938:Brazil; D002675:Child, Preschool; D003731:Dental Caries / Q000235:genetics*; D003733:Dental Caries Susceptibility / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry; D023083:beta-Defensins / Q000235:genetics*
C406407:DEFB1 protein, human; D035683:MicroRNAs; D023083:beta-Defensins
Caries; Human β-defensins; Polymorphism