Rectal suction biopsy with calretinin immunohistochemistry in patients suspected with residual aganglionosis after operation for Hirschsprung disease.
BACKGROUND
This study investigates the use of rectal suction biopsy (RSB) with calretinin immunohistochemical staining (CIS) in patients suspected of having abnormally innervated bowel after pull through operation for Hirschsprung disease (HD).


METHODS
This study was conducted in Children's Hospital 2, Ho Chi Minh City from January 2015 through June 2016. Patients suspected with abnormally innervated bowel after pull through operation for HD were submitted for a RSB with CIS. Following histological results, the patients underwent an appropriate treatment (redo pull through operation or non-operative medical management) and followed up at least 6 months to evaluate their outcomes.


RESULTS
Ten patients were enrolled in our study. The median age was 5.6 (range 1.4-20) years. The diagnosis of normally innervated bowel was made in five patients by showing positive reactivity of CIS on RSB, whereas five others were diagnosed with abnormally innervated bowel. Final diagnosis of the five latter patients was confirmed by analyses of the resected bowel after a redo pull through operation (including 4 cases with residual aganglionosis and one case with transition zone bowel).


CONCLUSIONS
RSB with CIS is a reliable and simple method for diagnosis of abnormally innervated bowel after pull through operation for HD.


METHODS
III TYPE OF STUDY: Study of diagnostic test (evaluate the effectiveness of a diagnostic test or outcome assessment).
Department of Pediatric Surgery, Children's Hospital 2, 14 Ly Tu Trong St., Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam; Laboratory of Pediatrics, Queen Fabiola Children's University Hospital, Université Libre de Bruxelles (ULB), Avenue J.J. Crocq 15 - 1020, Brussels, Belgium. Electronic address: dr.tranquocviet@gmail.com.
Viet Quoc
VQ
Tran
Department of Pediatric Surgery, Children's Hospital 2, 14 Ly Tu Trong St., Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Dinh Quang
DQ
Truong
Department of Pediatric Surgery, Queen Fabiola Children's University Hospital, Université Libre de Bruxelles (ULB), Avenue J.J. Crocq 15 - 1020, Brussels, Belgium.
Philippe
P
Goyens
Laboratory of Pediatrics, Queen Fabiola Children's University Hospital, Université Libre de Bruxelles (ULB), Avenue J.J. Crocq 15 - 1020, Brussels, Belgium.
Henri
H
Steyaert
D000293:Adolescent; D001706:Biopsy / Q000379:methods; D064032:Calbindin 2 / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D013505:Digestive System Surgical Procedures; D005260:Female; D006627:Hirschsprung Disease / Q000378:metabolism* / Q000601:surgery*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D017063:Outcome Assessment, Health Care; D012007:Rectum / Q000737:chemistry* / Q000473:pathology; D013396:Suction; D055815:Young Adult
D064032:Calbindin 2
Calretinin immunohistochemistry; Hirschsprung disease; Rectal suction biopsy; Residual aganglionosis; Transition zone bowel