Analysis of cannabinoids by liquid chromatography-mass spectrometry in milk, liver and hemp seed to ensure food safety.
A method for determining cannabinoids, Δ9-tetrahidrocannabinol (THC), 11-nor-9-carboxy-Δ9-THC (THC-COOH) and 11-hidroxy-Δ9-THC (THC-OH) in milk, liver and hemp seeds based on liquid chromatography tandem mass spectrometry has been optimized and validated. Analytes were extracted with methanol and the extracts cleaned-up by solid-phase extraction using Oasis HLB (60mg). The developed method was validated according to the Commission Decision 2002/657/EC. The decision limit (CCα) and detection capability (CCβ) ranged from 3.10-10.5ngg-1and 3.52-11.5ngg-1, the recoveries were 76-118% and matrix effect ranged from -17.8% to 19.9% in the three matrices studied. The method was applied to food samples obtaining positive results for THC in hemp seeds (average 0.82μgg-1) and three brands of junior formula milk at concentrations from 4.76 to 56.11ngg-1. The developed method was suitable achieving identification and quantification of cannabinoids in food matrices.
Environmental and Food Safety Research Group (SAMA-UV), Desertification Research Centre CIDE (CSIC-UV-GV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, Spain.
Úrsula
Ú
Escrivá
Environmental and Food Safety Research Group (SAMA-UV), Desertification Research Centre CIDE (CSIC-UV-GV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, Spain. Electronic address: m.jesus.andres@uv.es.
María Jesús
MJ
Andrés-Costa
Lanscape Chemistry and Environmental Forensics Group, CIDE (CSIC-UV-GV), Carretera Moncada, Náquera, Km. 4.5, Moncada, 46113 Valencia, Spain.
Vicente
V
Andreu
Environmental and Food Safety Research Group (SAMA-UV), Desertification Research Centre CIDE (CSIC-UV-GV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, Spain.
Yolanda
Y
Picó
D000818:Animals; D002186:Cannabinoids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D059022:Food Safety / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods
D002186:Cannabinoids
Cannabinoids; Food of animal origin; Food safety; Hemp seed; LC–MS/MS; Solid-phase extraction