Gordonia sp.: a salt tolerant bacterial inoculant for growth promotion of pearl millet under saline soil conditions.
In the resent study, a diazotrophic bacterial isolate JPA2 having the ability to tolerate salinity (6 % NaCl) and plant growth-promoting features was isolated from rhizospheric soil of weed Chenopodium murale growing in saline soil of Pindara (EC 11.47 dS m-1), district Jind (Haryana). The nitrogen fixing ability of the isolate was confirmed by nifH gene amplification and acetylene reduction assay (38.9 nmol ethylene h-1mg-1protein). The potential of strain JPA2 to promote growth of pearl millet was investigated by inoculation experiment which showed significant increase in plant height (51.1, 39.9 and 28.8 %) and dry weight (55.9, 36.4 and 35.5 %) over uninoculated control plants at EC 0, 6, 8 dS m-1, respectively. The strain JPA2 was Gram +ve and identified as Gordonia sp. on the basis of partial 16S rRNA gene sequencing and biochemical characterization. It is concluded that salt tolerant diazotrophic Gordonia sp. can be considered as a beneficial microbe for agriculture in saline soils.
Department of Microbiology, CCS Haryana Agricultural University, Hisar, 125004, India.
Monika
M
Kayasth
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, 173234, Himachal Pradesh, India.
Varun
V
Kumar
Department of Microbiology, CCS Haryana Agricultural University, Hisar, 125004, India. rajeshgera1967@gmail.com.
Rajesh
R
Gera
Gordonia sp.; Pearl millet; Plant growth-promoting rhizobacterium; Salt tolerant; nifH