Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies.
To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types.
nanoTherics Limited, Keele University Science Park, Keele, ST5 5NL, UK. mahendran@nanotherics.com.
Mahendran
M
Subramanian
nanoTherics Limited, Keele University Science Park, Keele, ST5 5NL, UK. aimeejayne@nanotherics.com.
Aimee-Jayne
AJ
Tyler
nanoTherics Limited, Keele University Science Park, Keele, ST5 5NL, UK. eva@nanotherics.com.
Eva Maria
EM
Luther
Takeda Cambridge, 418 Cambridge Science Park Milton Rd, Milton, Cambridge, CB4 0PZ, UK. elena.didaniel@ndm.ox.ac.uk.
Elena Di
ED
Daniel
Biological and Environmental Sciences, School of Natural Sciences, University of Stirling, Stirling, FK9 4LA, UK. jenson.lim@stir.ac.uk.
Jenson
J
Lim
J. Crayton Pruitt Family Department of Biomedical Engineering, Department of Material Science and Engineering, and Institute for Cell Engineering and Regenerative Medicine-ICERM, University of Florida, P.O. Box 116131, Gainesville, Florida, FL 32611, USA. jdobson@bme.ufl.edu.
Jon
J
Dobson
SH-SY5Y; biomagnetics; cortical neurons; gradient magnetic field; hippocampal neurons; magnetic nanoparticles; nanomagnetic gene transfection; non-viral gene delivery; permanent magnets; transfection