Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks.
OBJECTIVE
To investigate various biochemical components of seminal plasma in Marwari stallions and Poitou Jacks and to find out their correlation with that of the seminal characteristics.


METHODS
In this study, semen was collected from six Marwari stallions and six Poitou jacks aged from 4 to 6 years and with known fertility status. The semen collection from the stallions were collected during the breeding season, i.e., between the months of April and June. From the collected semen ejaculates, we estimated the values of some biochemical components, viz., total protein content, total lipid content, and enzymes such as glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), alkaline phosphatase (ALP), acid phosphatase (ACP), and lactate dehydrogenase (LDH) as well as concentrations of glucose, cholesterol, total calcium (Ca), and phosphorus (P) and correlations among different seminal parameters were statistically examined using the Pearson correlation coefficient.


RESULTS
In this study, we found positive correlations between semen volume as well as sperm concentration and GOT, GPT, ALP and ACP for both the group stallions. Significant correlation between motility and glucose, GOT and GPT could be an indication for their role metabolism and protection against free radicals to the spermatozoa.


CONCLUSIONS
Based on the results, it is concluded that there is a positive correlation between some biochemical values such as glucose, Ca, ALP, and LDH and seminal parameters which play a key role in capacitation and onward movement of the spermatozoa.
Department of Animal Reproduction, Equine Production Campus, Indian Council of Agricultural Research-National Research Centre on Equines, Bikaner, Rajasthan-334001.
Thirumala Rao
TR
Talluri
Department of Biochemistry, Biochemistry Laboratory, Indian Council of Agricultural Research - Indian Veterinary Research Institute, Palampur - 176 061, Himachal Pradesh, India.
Gorakh
G
Mal
Department of Animal Reproduction, Equine Production Campus, Indian Council of Agricultural Research-National Research Centre on Equines, Bikaner, Rajasthan-334001.
Sanjay Kumar
SK
Ravi
Marwari; Poitou; alkaline phosphatase; biochemical components; lactate-dehydrogenase; seminal plasma; stallion