Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord.
Mesenchymal stromal cells (MSCs) are being investigated as a potential alternative for cellular therapy. This study was designed to compare the biological characteristics of MSCs isolated from amniotic membrane (A-MSCs), chorionic membrane (C-MSCs), placental decidua (D-MSCs) and umbilical cord (UC-MSCs) to ascertain whether any one of these sources is superior to the others for cellular therapy purposes.



MSCs were isolated from amniotic membrane, chorionic membrane, umbilical cord and placental decidua. Immunophenotype, differentiation ability, cell size, cell complexity, polarity index and growth kinetics of MSCs isolated from these four sources were analyzed.



MSCs were successfully isolated from all four sources. Surface marker profile and differentiation ability were consistent with human MSCs. C-MSCs in suspension were the smallest cells, whereas UC-MSCs presented the greatest length and least width. A-MSCs had the lowest polarity index and UC-MSCs, as more elongated cells, the highest. C-MSCs, D-MSCs and UC-MSCs exhibited similar growth capacity until passage 8 (P8); C-MSCs presented better lifespan, whereas insignificant proliferation was observed in A-MSCs.



Neonatal and maternal tissues can serve as sources of multipotent stem cells. Some characteristics of MSCs obtained from four neonatal tissues were compared and differences were observed. Amniotic membrane was the least useful source of MSCs, whereas chorionic membrane and umbilical cord were considered good options for future use in cell therapy because of the known advantages of immature cells.
Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil; Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: anelise_araujo@yahoo.com.br.
Anelise Bergmann
AB
Araújo
Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Gabrielle Dias
GD
Salton
Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Juliana Monteiro
JM
Furlan
Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Natália
N
Schneider
Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Melissa Helena
MH
Angeli
Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Álvaro Macedo
ÁM
Laureano
Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Lúcia
L
Silla
Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Eduardo Pandolfi
EP
Passos
Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Ana Helena
AH
Paz
D000650:Amnion / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology*; D003656:Decidua / Q000166:cytology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007231:Infant, Newborn; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011247:Pregnancy; D014470:Umbilical Cord / Q000166:cytology*
amniotic membrane; cell therapy; chorionic membrane; human placenta; mesenchymal stromal cell sources; mesenchymal stromal cells; neonatal tissues; placental decidua; umbilical cord