Phototherapeutic spectrum expansion through synergistic effect of mesoporous silica trio-nanohybrids against antibiotic-resistant gram-negative bacterium.
The extensive impact of antibiotic resistance has led to the exploration of new anti-bacterial modalities. We designed copper impregnated mesoporous silica nanoparticles (Cu-MSN) with immobilizing silver nanoparticles (SNPs) to apply photodynamic inactivation (PDI) of antibiotic-resistant E. coli. SNPs were decorated over the Cu-MSN surfaces by coordination of silver ions on diamine-functionalized Cu-MSN and further reduced to silver nanoparticles with formalin. We demonstrate that silver is capable of sensitizing the gram-negative bacteria E. coli to a gram-positive specific phototherapeutic agent in vitro; thereby expanding curcumin's phototherapeutic spectrum. The mesoporous structure of Cu-MSN remains intact after the exterior decoration with silver nanoparticles and subsequent curcumin loading through an enhanced effect from copper metal-curcumin affinity interaction. The synthesis, as well as successful assembly of the functional nanomaterials, was confirmed by various physical characterization techniques. Curcumin is capable of producing high amounts of reactive oxygen species (ROS) under light irradiation, which can further improve the silver ion release kinetics for antibacterial activity. In addition, the positive charged modified surfaces of Cu-MSN facilitate antimicrobial response through electrostatic attractions towards negatively charged bacterial cell membranes. The antibacterial action of the synthesized nanocomposites can be activated through a synergistic mechanism of energy transfer of the absorbed light from SNP to curcumin.
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
Yaswanth
Y
Kuthati
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan; College of Chemical Engineering, Huaqiao University, Xiamen, China.
Ranjith Kumar
RK
Kankala
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
Prabhakar
P
Busa
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
Shi-Xiang
SX
Lin
Department of Chemistry, Tamkang University, New Taipei City 251, Taiwan.
Jin-Pei
JP
Deng
Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
Chung-Yuan
CY
Mou
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan. Electronic address: chlee016@gms.ndhu.edu.tw.
Chia-Hung
CH
Lee
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry*; D003300:Copper; D003474:Curcumin / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000528:radiation effects; D004357:Drug Synergism; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000528:radiation effects; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D053761:Nanocomposites / Q000737:chemistry / Q000627:therapeutic use; D010789:Phototherapy / Q000379:methods / Q000592:standards*; D011838:Radiation-Sensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000528:radiation effects; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver
D000900:Anti-Bacterial Agents; D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species; D012834:Silver; D012822:Silicon Dioxide; D003300:Copper; D003474:Curcumin
Antibiotic resistance; Mesoporous silica; Nanoparticles; Photodynamic inactivation; Silver; Synergism