Alterations in Gastric Microbiota After H. Pylori Eradication and in Different Histological Stages of Gastric Carcinogenesis.
The role of bacteria other than Helicobacter pylori (HP) in the stomach remains elusive. We characterized the gastric microbiota in individuals with different histological stages of gastric carcinogenesis and after receiving HP eradication therapy. Endoscopic gastric biopsies were obtained from subjects with HP gastritis, gastric intestinal metaplasia (IM), gastric cancer (GC) and HP negative controls. Gastric microbiota was characterized by Illumina MiSeq platform targeting the 16 S rDNA. Apart from dominant H. pylori, we observed other Proteobacteria including Haemophilus, Serratia, Neisseria and Stenotrophomonas as the major components of the human gastric microbiota. Although samples were largely converged according to the relative abundance of HP, a clear separation of GC and other samples was recovered. Whilst there was a strong inverse association between HP relative abundance and bacterial diversity, this association was weak in GC samples which tended to have lower bacterial diversity compared with other samples with similar HP levels. Eradication of HP resulted in an increase in bacterial diversity and restoration of the relative abundance of other bacteria to levels similar to individuals without HP. In conclusion, HP colonization results in alterations of gastric microbiota and reduction in bacterial diversity, which could be restored by antibiotic treatment.
Department of Medicine, The University of Hong Kong, Hong Kong.
Tung Hiu
TH
Li
Centre for Genomic Sciences, The University of Hong Kong, Hong Kong.
Youwen
Y
Qin
Centre for Genomic Sciences, The University of Hong Kong, Hong Kong.
Pak Chung
PC
Sham
Department of Medicine, The University of Hong Kong, Hong Kong.
K S
KS
Lau
Department of Surgery, The University of Hong Kong, Hong Kong.
Kent-Man
KM
Chu
Department of Medicine, The University of Hong Kong, Hong Kong.
Wai K
WK
Leung
D044822:Biodiversity; D002471:Cell Transformation, Neoplastic; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology* / Q000502:physiology*; D016481:Helicobacter Infections / Q000150:complications* / Q000382:microbiology*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D064307:Microbiota; D013274:Stomach Neoplasms / Q000209:etiology* / Q000473:pathology*