Short Communication: Manα1-2Man-Binding Anti-HIV Lectins Enhance the Exposure of V2i and V3 Crown Neutralization Epitopes on the V1/V2 and V3 Hypervariable Loops of HIV-1 Envelope.
This study aimed to explore the contribution of high-mannose glycans in the masking of conserved V3 crown (GPG) and V2i epitopes on the hypervariable loops of most exposed distal surface of HIV-1 Env. Using lectins specific to Manα1-2Man residue containing Man6-9GlcNAc2glycans extensively decorating HIV-1 Env, we found that Manα1-2Man-binding lectins enhance the exposure of these partially and transiently exposed epitopes and consequentially increase the neutralization strength of antibodies against these epitopes.
1 Department of Immunopathology, Post Graduate Institute of Medical Education and Research , Chandigarh, India .|2 Department of Infectious Diseases, Icahn School of Medicine , Mount Sinai, New York.
Muzafar
M
Jan
2 Department of Infectious Diseases, Icahn School of Medicine , Mount Sinai, New York.
Chitra
C
Upadhyay
3 Department of Internal Medicine, Post Graduate Institute of Medical Education and Research , Chandigarh, India .
Aman
A
Sharma
2 Department of Infectious Diseases, Icahn School of Medicine , Mount Sinai, New York.
Catarina E
CE
Hioe
1 Department of Immunopathology, Post Graduate Institute of Medical Education and Research , Chandigarh, India .
Sunil K
SK
Arora
D004187:Disaccharides / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D037102:Lectins / Q000276:immunology*; D008358:Mannose / Q000276:immunology*; D008362:Mannosides / Q000276:immunology; D009500:Neutralization Tests / Q000379:methods; D011134:Polysaccharides / Q000276:immunology
D004187:Disaccharides; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D037102:Lectins; D008362:Mannosides; D011134:Polysaccharides; C475460:mannosyl alpha(1-6)-mannoside; D008358:Mannose
HIV-1; N-linked glycans; anti-HIV lectins; envelope; hypervariable loop epitopes