Early response of wheat antioxidant system with special reference to Fusarium head blight stress.
Fusarium head blight (FHB) is a destructive fungal disease of wheat (Triticum aestivum L.) that causes significant grain yield losses and end-use quality reduction associated with contamination by the mycotoxin deoxynivalenol (DON). Three winter wheat varieties ('Vulkan', 'Kraljica' and 'Golubica') were screened for FHB resistance using artificial inoculation technique under field conditions. The aim of this study was to examine a relationship between FHB resistance and the effectiveness of enzyme antioxidant system of wheat varieties under different sampling times (3, 15, 24, 48, 96, 120 and 336 hai). In the time-course experiments FHB-resistant variety 'Vulkan' showed rapid induction of ascorbate peroxidase (APX) and polyphenol oxidase (PPO) activity in the early stages after infection (3 hai) and it seems that in 'Vulkan' FHB-resistance is associated with antioxidative enzymes activity. Moderately FHB resistant variety 'Kraljica' showed the higher guaiacol peroxidase (POD) activity and higher H2O2content after 24 hai, increased malondialdehyde (MDA) content at the beginning of infection (3, 15 hai) while induction of catalase (CAT), APX and PPO was delayed. FHB-susceptible variety 'Golubica' involved antioxidant enzymes in defense response much later. Based on our results the activity of antioxidant enzymes (APX and PPO) was more pronounced in 'Vulkan' than in FHB-medium resistant variety 'Kraljica' and FHB-susceptible 'Golubica'. The differences in antioxidant response of wheat varieties under Fusarium infestation could be the result of genetic properties.
Agricultural Institute Osijek, Juzno Predgradje 17, HR-31000 Osijek, Croatia. Electronic address: valentina.spanic@poljinos.hr.
Valentina
V
Spanic
Agricultural Institute Osijek, Juzno Predgradje 17, HR-31000 Osijek, Croatia.
Marija
M
Viljevac Vuletic
Agricultural Institute Osijek, Juzno Predgradje 17, HR-31000 Osijek, Croatia.
Ivan
I
Abicic
Subdepartment of Biology and Microbiology, Faculty of Food Technology Osijek, Franje Kuhaca 20, HR-31000 Osijek, Croatia.
Tihana
T
Marcek
D000975:Antioxidants / Q000378:metabolism*; D005670:Fusarium; D015971:Gene Expression Regulation, Enzymologic; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000378:metabolism / Q000382:microbiology*
D000975:Antioxidants; D010940:Plant Proteins
Antioxidant system; Fusarium; Wheat