Enhanced production of chitin deacetylase by Penicillium oxalicum SAEM-51 through response surface optimization of fermentation conditions.
Optimization of the fermentation conditions for chitin deacetylase (CDA) production by Penicillium oxalicum SAEM-51 was undertaken in the present study using central composite design (CCD) under submerged condition. CDA is widely employed for bio-catalytic conversion of chitin to chitosan. Chitosan is a biopolymer with immense commercial potential in diverse industrial sectors, viz. pharmaceutics, food, agriculture, water treatment, etc. CDA production was significantly affected by all the variables studied, viz. pH, temperature, inoculum age and size. The optimal conditions that stimulating maximal CDA production were found to be: pH, 7.9; temperature, 28 °C; inoculum age, 90 h, and 11 % inoculum size. Under these optimized conditions, the actual maximal CDA production was 623.57 ± 8.2 Ul-1, which was in good agreement with the values predicted by the quadratic model (648.24 Ul-1), confirming the validity of the model. Optimization of fermentation conditions through CCD had resulted into 1.4-fold enhancement in CDA productivity (Qp= 4.3264 Ul-1h-1). Results of these experiments indicated that response surface methodology was proved to be a promising method for optimization of CDA production.
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India. nidhipareek2006@gmail.com.|Department of Chemistry, Umeå University, 90183, Umeå, Sweden. nidhipareek2006@gmail.com.
Nidhi
N
Pareek
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India. ghoshfbs@iitr.ernet.in.
Sanjoy
S
Ghosh
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India.
R P
RP
Singh
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India.|Protein Engineering and Proteomics Group, Department of Chemistry, Biotechnology and Food Sciences, Norwegian University of Life Sciences, 1430, Ås, Norway.
V
V
Vivekanand
Central composite design; Chitin deacetylase; Chitosan; Fermentation conditions; Penicillium oxalicum