Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening.
Applying the right acquisition method in a fluorescence imaging-based screening context is of great importance to obtain an appropriate readout and to select the right scale of the screen. In order to save imaging time and data, we have developed routines for multiscale targeted imaging, providing both a broad overview of a sample and additional in-depth information for targets of interest identified within the screen. These objects can be identified and acquired on-the-fly by an interconnection of image acquisition and image analysis.
BioQuant Center, ViroQuant-CellNetworks RNAi Screening Facility, University of Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany. manuel.gunkel@bioquant.uni-heidelberg.de.
Manuel
M
Gunkel
BioQuant Center, ViroQuant-CellNetworks RNAi Screening Facility, University of Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany.
Jan Philipp
JP
Eberle
BioQuant Center, ViroQuant-CellNetworks RNAi Screening Facility, University of Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany.
Holger
H
Erfle
D015415:Biomarkers; D002460:Cell Line; D017930:Genes, Reporter; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010641:Phenotype; D012984:Software; D057188:Workflow
D015415:Biomarkers
Automated imaging; High content screening; High-throughput screening; KNIME; Targeted imaging