Exploring novel sero-epidemiological tools-Effect of different storage conditions on longitudinal stability of microarray slides comprising influenza A-, measles- and Streptococcus pneumoniae antigens.
In this study we evaluated the long-term stability of a microarray-based serological screening platform, containing antigens of influenza A, measles and Streptococcus pneumoniae, as part of a preparedness research program aiming to develop assays for syndromic disease detection. Spotted microarray slides were kept at four different storage regimes with varying temperature and humidity conditions. We showed that under the standard storage condition in a temperature-controlled (21°C) and desiccated environment (0% relative humidity), microarray slides remained stable for at least 22 months without loss of antigen quality, whereas the other three conditions (37°C, desiccated; Room temperature, non-desiccated; Frozen, desiccated) produced acceptable results for some antigens (influenza A, S.pneumoniae), but not for others (measles). We conclude that these arrays for multiplex antibody testing can be prepared and stored for prolonged periods of time, which aids laboratory-preparedness and facilitates sero-epidemiological studies.
Viroscience Department, Erasmus Medical Center, Rotterdam, the Netherlands; Virology Department, Centre for Infectious Diseases Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3721 MA Bilthoven, the Netherlands. Electronic address: gudrun.freidl@rivm.nl.
Gudrun S
GS
Freidl
Viroscience Department, Erasmus Medical Center, Rotterdam, the Netherlands; Virology Department, Centre for Infectious Diseases Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3721 MA Bilthoven, the Netherlands. Electronic address: e.debruin@erasmusmc.nl.
Erwin de
E
Bruin
Department for Statistics, Informatics and Mathematical Modelling, National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3721 MA Bilthoven, the Netherlands. Electronic address: maarten.schipper@rivm.nl.
Maarten
M
Schipper
Viroscience Department, Erasmus Medical Center, Rotterdam, the Netherlands; Virology Department, Centre for Infectious Diseases Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3721 MA Bilthoven, the Netherlands. Electronic address: m.koopmans@erasmusmc.nl.
Marion
M
Koopmans
D000942:Antigens, Bacterial / Q000737:chemistry*; D000956:Antigens, Viral / Q000737:chemistry*; D003890:Desiccation; D004355:Drug Stability; D004356:Drug Storage; D006801:Humans; D007159:Immunologic Tests / Q000295:instrumentation; D009980:Influenza A virus / Q000737:chemistry; D007251:Influenza, Human / Q000175:diagnosis; D008457:Measles / Q000175:diagnosis; D008459:Measles virus / Q000737:chemistry; D011008:Pneumococcal Infections / Q000175:diagnosis; D040081:Protein Array Analysis; D016036:Seroepidemiologic Studies; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000942:Antigens, Bacterial; D000956:Antigens, Viral
Antigen; Humidity; Microarray; Serology; Stability; Storage; Temperature