Detection of Polish clinical Aspergillus fumigatus isolates resistant to triazoles.
We studied the presence of triazole resistance of 121 Aspergillus fumigatus clinical isolates collected in two Polish cities, Warsaw and Wrocław, to determine if resistance is emerging in our country. We identified five itraconazole resistant isolates (4.13%) carrying the TR34/L98H alteration in Cyp51A gene, four of which were cross-resistant to posaconazole and one to voriconazole. One isolate was intermediate susceptible to itraconazole and harbored no Cyp51A alterations. The study confirms the presence of azole resistant A. fumigatus strains in Poland at a level that is comparative to other European countries.
Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 213, 50-566 Wroclaw, Poland.
Urszula
U
Nawrot
Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland.
Ewelina
E
Kurzyk
Unit of Mycology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark.|Department of Clinical Microbiology, Rigshospitalet, Copenhagen University, Copenhagen, Denmark.
Maiken Cavling
MC
Arendrup
Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland.
Martyna
M
Mroczynska
Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 213, 50-566 Wroclaw, Poland.
Katarzyna
K
Wlodarczyk
Department of Microbiology, Central Clinical Hospital in Warsaw, Medical University of Warsaw, Banacha 1A, 02-097 Warsaw, Poland.
Beata
B
Sulik-Tyszka
Department of Microbiology, Central Clinical Hospital in Warsaw, Medical University of Warsaw, Banacha 1A, 02-097 Warsaw, Poland.|Department of Dental Microbiology, Medical University of Warsaw, Banacha 1A, 02-097 Warsaw, Poland.
Marta
M
Wróblewska
Department of Pediatric Bone Marrow Transplantation, Oncology and Hematology, Wroclaw Medical University Borowska 213, 50-556 Wroclaw, Poland.
Marek
M
Ussowicz
Department of Immunology and Infectious Diseases, Institute of Immunology and Experimental Therapy, Weigla 12, 53-114 Wroclaw, Poland.
Przemyslaw
P
Zdziarski
Department of Anesthesiology and Intensive Therapy, Paediatric Intensive Care Unit Wroclaw Medical University, Chalubinskiego 1A, 50-368 Wroclaw, Poland.
Kinga
K
Niewinska
Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland.
Anna
A
Brillowska-Dabrowska
D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000302:isolation & purification*; D002947:Cities; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D025141:Drug Resistance, Fungal; D005656:Fungal Proteins / Q000235:genetics; D005838:Genotype; D006801:Humans; D020125:Mutation, Missense; D011044:Poland; D014230:Triazoles / Q000494:pharmacology*
D000935:Antifungal Agents; D005656:Fungal Proteins; D014230:Triazoles; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus
Aspergillus fumigatus; TR34/L98H; azole resistance; environmental genotype; mutations in Cyp51A