Pseudomonas aeruginosa cytochrome c551denaturation by five systematic urea derivatives that differ in the alkyl chain length.
Reversible denaturation of Pseudomonas aeruginosa cytochrome c551(PAc551) could be followed using five systematic urea derivatives that differ in the alkyl chain length, i.e. urea, N-methylurea (MU), N-ethylurea (EU), N-propylurea (PU), and N-butylurea (BU). The BU concentration was the lowest required for the PAc551denaturation, those of PU, EU, MU, and urea being gradually higher. Furthermore, the accessible surface area difference upon PAc551denaturation caused by BU was found to be the highest, those by PU, EU, MU, and urea being gradually lower. These findings indicate that urea derivatives with longer alkyl chains are stronger denaturants. In this study, as many as five systematic urea derivatives could be applied for the reversible denaturation of a single protein, PAc551, for the first time, and the effects of the alkyl chain length on protein denaturation were systematically verified by means of thermodynamic parameters.
a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
Shinya
S
Kobayashi
a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
Sotaro
S
Fujii
a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
Aya
A
Koga
b Graduate School of Science Technology and Innovation , Kobe University , Kobe , Japan.
Satoshi
S
Wakai
c Graduate School of Engineering Science , Osaka University , Osaka , Japan.|d Elements Strategy Initiative for Catalysts and Batteries , Kyoto University , Kyoto , Japan.
Nobuyuki
N
Matubayasi
a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
Yoshihiro
Y
Sambongi
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003574:Cytochrome c Group / Q000737:chemistry* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008782:Methylurea Compounds / Q000737:chemistry* / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014508:Urea / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D008782:Methylurea Compounds; D011994:Recombinant Proteins; C017680:propylurea; C007685:ethylurea; D014508:Urea; C017167:cytochrome C(551); C006865:butylurea; C002352:methylurea
Pseudomonas aeruginosa; cytochrome c; protein denaturation; urea derivatives