PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents-Synthesis, physicochemical characterization and in vitro evaluation.
We describe the synthesis of cellulose nanocrystals (CNCs) complexed to siRNA. PEI (600Da) was first covalently attached to CNCs by reductive amination reaction, then CNCs-PEI were loaded with siRNA, resulting in the formation of CNCs-PEI-siRNA particles, strongly stabilized by ionic interactions. Efficient cellular uptake of these particles was monitored by ethidium bromide staining. Not only did CNCs-PEI show no cytotoxicity at the studied concentrations, but they also protected siRNA from degradation and favored its delivery into the cells. This siRNA (siRNA killer) is able to silence the expression of cell cycle genes and to induce cell death by apoptosis. Therefore, this study suggests that these CNCs-PEI are promising non-viral nanovehicles for siRNA delivery and for efficient anti-tumor strategy.
Université de Limoges, LCSN, EA 1069, F-87000 Limoges, France.
Gautier M A
GM
Ndong Ntoutoume
Université de Limoges, UGMA, INRA, F-87000 Limoges, France.
Vincent
V
Grassot
Université de Limoges, LCSN, EA 1069, F-87000 Limoges, France.
Frédérique
F
Brégier
Université de Limoges, UGMA, INRA, F-87000 Limoges, France.
Julien
J
Chabanais
Université de Limoges, UGMA, INRA, F-87000 Limoges, France.
Jean-Michel
JM
Petit
Université de Limoges, LCSN, EA 1069, F-87000 Limoges, France.
Robert
R
Granet
Université de Limoges, LCSN, EA 1069, F-87000 Limoges, France. Electronic address: vincent.sol@unilim.fr.
Vincent
V
Sol
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002482:Cellulose / Q000737:chemistry*; D020868:Gene Silencing; D005822:Genetic Vectors; D051379:Mice; D053758:Nanoparticles; D011094:Polyethyleneimine / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry*
D034741:RNA, Small Interfering; D011094:Polyethyleneimine; D002482:Cellulose
Cellulose nanocrystals; Nanoparticles; PEI; Transfected cells; siRNA delivery