Isolation, characterization and bioactivities of the polysaccharides from Dicliptera chinensis (L.) Juss.
The polysaccharides of Dicliptera chinensis (L.) Juss. (DCP-1 and DCP-2) were extracted and isolated using the methods of water extract-ethanol precipitate and sephadex column chromatography and characterized by gel permeation chromatography (GPC), Fourier transform infrared spectrometry (FT-IR) and gas chromatography (GC), respectively. The antioxidant activity of DCPs was evaluated by scavenging activity of DPPH, hydroxyl, superoxide anion and ABTS radical. Moreover, the anti-aging activity of DCP-2 was investigated using an aging model-induced by D-galactose (D-gal) in mice. The results show that the weight average molecular weight (Mw) of DCP-2 was 2 273Da with a narrow polydispersity index of 1.01, and it was a heteropolysaccharide and consisted of glucose, galactose, arabinose, rhamnose and mannose with a molar ratio of 3.20:2.54:1.69:1.58:1.00. DCP-2 had stronger antioxidant activity against DPPH, hydroxyl, superoxide anion and ABTS radical, while DCP-1 had hardly any antioxidant activity and DCP had weaker antioxidant activity. Furthermore, DCP-2 can enhance antioxidant capacity and had anti-aging activity against D-gal induced aging mice. These results proposed that DCP-2 might be developed as a potential functional food with the activity of anti-aging.
School of Pharmacy, Guilin Medical University, Guilin 541004, China.
Yourui
Y
Xu
School of Pharmacy, Guilin Medical University, Guilin 541004, China.
Ya
Y
Gao
School of Pharmacy, Guilin Medical University, Guilin 541004, China.
Mingli
M
Zhong
Department of Obstetrics & Gynecology, National University of Singapore, 119074, Singapore.
Jun
J
Li
School of Pharmacy, Guilin Medical University, Guilin 541004, China.
Houkang
H
Cao
School of Pharmacy, Guilin Medical University, Guilin 541004, China.
Simao
S
Huang
School of Pharmacy, Guilin Medical University, Guilin 541004, China. Electronic address: 113038799@qq.com.
Riming
R
Wei
School of Pharmacy, Guilin Medical University, Guilin 541004, China. Electronic address: xueshengcailiao@163.com.
Kefeng
K
Zhang
D000375:Aging / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D005260:Female; D051379:Mice; D008970:Molecular Weight; D028201:Pinaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D011134:Polysaccharides; D014867:Water; D000431:Ethanol
Anti-aging activity; Antioxidant activity; Dicliptera chinensis (L.) Juss.