Investigation of silk fibroin nanoparticle-decorated poly(l-lactic acid) composite scaffolds for osteoblast growth and differentiation.
Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(l-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment. The in vitro cytotoxicity test of SF/PLLA scaffolds in L929 mouse fibroblast cells indicated good biocompatibility. Then, the in vitro interplay between mouse preosteoblast cell (MC3T3-E1) and various topological structures and biochemical cues were evaluated. The cell adhesion, proliferation, osteogenic differentiation and their relationship with the structures as well as SF content were explored. The SF/PLLA weight ratio (2:8) significantly affected the MC3T3-E1 cells by improving the expression of key players in the regulation of bone formation, ie, alkaline phosphatase (ALP), osteocalcin (OC) and collagen 1 (COL-1). These results suggest not only the importance of surface topography and biochemical cues but also the potential of applying SF/PLLA composite scaffolds as biomaterials in bone tissue engineering.
Institute of Biomaterials and Tissue Engineering.
Biao-Qi
BQ
Chen
Institute of Biomaterials and Tissue Engineering; Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian.
Ranjith Kumar
RK
Kankala
Institute of Biomaterials and Tissue Engineering; Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian.
Ai-Zheng
AZ
Chen
Institute of Biomaterials and Tissue Engineering.
Ding-Zhu
DZ
Yang
Institute of Biomaterials and Tissue Engineering.
Xiao-Xia
XX
Cheng
Institute of Biomaterials and Tissue Engineering; Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian.
Ni-Na
NN
Jiang
Department of Cardiac Surgery, Zhongshan Hospital, Fudan University; Shanghai Institute of Cardiovascular Disease, Shanghai, People's Republic of China.
Kai
K
Zhu
Institute of Biomaterials and Tissue Engineering; Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian.
Shi-Bin
SB
Wang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D005349:Fibroins / Q000494:pharmacology*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D024042:Collagen Type I; D011091:Polyesters; D015675:Osteocalcin; C033616:poly(lactide); D003094:Collagen; D005349:Fibroins; D000469:Alkaline Phosphatase
alkaline phosphatase; bone engineering; cellular adhesion; super critical fluids; surface topography