Protection and antibody reactivity following vaccination of lumpfish (Cyclopterus lumpus L.) against atypical Aeromonas salmonicida.
Atypical Aeromonas salmonicida is frequently associated with disease and mortality in farmed lumpfish (Cyclopterus lumpus L). Challenge experiments using different modes of exposure identified both high and low pathogenic isolates. Intraperitoneal vaccination induced production of high levels of specific antibodies particularly in fish given multiple injections. The immune sera contained antibodies cross reactive with both high and low pathogenic isolates. SDS-PAGE and LC/MSMS analyses showed that the highly virulent isolate expressed the virulence array protein (A-layer) while the less virulent isolate did not. Vaccines, containing the highly virulent isolate, formulated as a monovalent or as a trivalent vaccine, provided 73 and 60 relative percent survival (RPS) respectively, following intraperitoneal challenge. The detection of high levels of specific antibodies in immune sera and the protection provided by the test vaccines strongly indicate that it is possible to vaccinate lumpfish against atypical A. salmonicida and most probably also against other infectious bacterial diseases.
Department of Biology, University of Bergen, Bergen High-Technology Centre, PO Box 7803, NO-5020 Bergen, Norway. Electronic address: anita.ronneseth@uib.no.
Anita
A
Rønneseth
Department of Biology, University of Bergen, Bergen High-Technology Centre, PO Box 7803, NO-5020 Bergen, Norway.
Gyri T
GT
Haugland
Department of Biology, University of Bergen, Bergen High-Technology Centre, PO Box 7803, NO-5020 Bergen, Norway; Norwegian Veterinary Institute, Oslo, Norway.
Duncan J
DJ
Colquhoun
PHARMAQ, Oslo, Norway.
Espen
E
Brudal
Department of Biology, University of Bergen, Bergen High-Technology Centre, PO Box 7803, NO-5020 Bergen, Norway.
Heidrun I
HI
Wergeland
D048409:Aeromonas salmonicida / Q000276:immunology* / Q000472:pathogenicity*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D005399:Fishes; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D007274:Injections, Intraperitoneal / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary*; D014774:Virulence
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines
A-layer; Adaptive immunity; Bacterial infection; Fish pathogen; Lumpfish; Mortality; Vaccination; vapA