Detection of Brucellosis in Sika Deer ( Cervus nippon ) through Loop-mediated Isothermal Amplification (LAMP).
Brucellosis (Brucella bovis) in sika deer ( Cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer. We developed a LAMP assay targeting the omp25 gene sequence to detect brucellosis in sika deer. The reaction can be completed in 60 min at 63 C and, with a detection limit of 17 pg, it was more sensitive than conventional PCR, with its detection limit of 1.7 ng. No cross-reactivity was observed with four bacteria: Escherichia coli , Salmonella enterica subsp. enterica, Clostridium pasteurianum , and Pseudomonas aeruginosa . We used 263 samples of blood to evaluate the reaction. The percentage of agreement between LAMP and PCR reached 91%; relative specificity reached 87%, and relative sensitivity reached 100%. The results indicate LAMP can be a simple and rapid diagnostic tool for detecting brucellosis in sika deer, particularly in the field, where it is essential to control brucellosis in deer with a rapid and accurate diagnosis for removal of positive animals.
1 Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.|2 Key Lab of Preventive Veterinary Medicine in Jilin Provence, Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.
Qianhong
Q
Liu
1 Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.
Jie
J
Wei
1 Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.
Qingsong
Q
Sun
1 Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.
Ben
B
Wang
1 Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.
Yuting
Y
Wang
1 Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.
Ying
Y
Hu
1 Jilin Agricultural Science and Technology University, No. 77, The Academician Road, Economic Development Zone, Jilin City, Jilin Province, 132101, People's Republic of China.
Wenrong
W
Wu
D000818:Animals; D002002:Brucella / Q000302:isolation & purification*; D002006:Brucellosis / Q000662:veterinary*; D003670:Deer / Q000821:virology*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D015047:Zoonoses
Brucella bovis; LAMP; brucellosis; loop-mediated isothermal amplification; sika deer