Characterization of wavefront errors in mouse cranial bone using second-harmonic generation.
Optical aberrations significantly affect the resolution and signal-to-noise ratio of deep tissue microscopy. As multiphoton microscopy is applied deeper into tissue, the loss of resolution and signal due to propagation of light in a medium with heterogeneous refractive index becomes more serious. Efforts in imaging through the intact skull of mice cannot typically reach past the bone marrow ( ? 150 ?? ? m of depth) and have limited resolution and penetration depth. Mechanical bone thinning or optical ablation of bone enables deeper imaging, but these methods are highly invasive and may impact tissue biology. Adaptive optics is a promising noninvasive alternative for restoring optical resolution. We characterize the aberrations present in bone using second-harmonic generation imaging of collagen. We simulate light propagation through highly scattering bone and evaluate the effect of aberrations on the point spread function. We then calculate the wavefront and expand it in Zernike orthogonal polynomials to determine the strength of different optical aberrations. We further compare the corrected wavefront and the residual wavefront error, and suggest a correction element with high number of elements or multiconjugate wavefront correction for this highly scattering environment.
University of Georgia, Regenerative Bioscience Center, Rhodes Center for ADS, Athens, Georgia, United States.
Kayvan Forouhesh
KF
Tehrani
University of Georgia, College of Engineering, Athens, Georgia, United States.
Peter
P
Kner
University of Georgia, Regenerative Bioscience Center, Rhodes Center for ADS, Athens, Georgia, United StatesbUniversity of Georgia, College of Engineering, Athens, Georgia, United States.
Luke J
LJ
Mortensen
D000465:Algorithms; D000818:Animals; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods*; D051379:Mice; D008853:Microscopy; D055095:Optics and Photonics; D012031:Refractometry; D012886:Skull / Q000000981:diagnostic imaging*