Comparison of the Protective Effects of Individual Components of Particulatedtrans-Sialidase (PTCTS), PTC and TS, against High Cholesterol Diet-Induced Atherosclerosis in Rabbits.
Previous studies showed the presence ofMycoplasma pneumoniae(M. pneumoniae) and membrane-shed microparticles (MPs) in vulnerable atherosclerotic plaques. H&S Science and Biotechnology developed PTCTS, composed by natural particles from medicinal plants (PTC) combined withtrans-Sialidase (TS), to combat MPs andMycoplasma pneumoniae. Our aim was to determine the effects of the different components of PTCTS in a rabbit model of atherosclerosis. Rabbits were fed with high cholesterol diet for 12 weeks and treated during the last 6 weeks with either vehicle, PTC, TS, or PTCTS. Lipid profile and quantification of MPs positive forMycoplasma pneumoniaeand oxidized LDL antigens were carried out. Aortas and organs were then histologically analyzed. PTCTS reduced circulating MPs positive forMycoplasma pneumoniaeand oxidized LDL antigens, reduced the plaque area in the abdominal aorta, and caused positive remodeling of the ascendant aorta. PTC caused positive remodeling and reduced plaque area in the abdominal aorta; however, TS had a lipid lowering effect. PTCTS components combined were more effective against atherosclerosis than individual components. Our data reinforce the infectious theory of atherosclerosis and underscore the potential role of circulating MPs. Therefore, the removal ofMycoplasma-derived MPs could be a new therapeutic approach in the treatment of atherosclerosis.
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Shérrira M
SM
Garavelo
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Maria de Lourdes
ML
Higuchi
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Jaqueline J
JJ
Pereira
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Marcia M
MM
Reis
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Joyce T
JT
Kawakami
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Renata N
RN
Ikegami
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Suely A P
SA
Palomino
Laboratory of Cardiac Pathology, Heart Institute, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Nilsa S Y
0000-0001-7566-0212
NS
Wadt
Pharmaceutical Sciences Section, College of Pharmacy, Qatar University, P.O. Box 2713, Doha, Qatar.
Abdelali
0000-0002-8363-1582
A
Agouni
D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects / Q000473:pathology; D050197:Atherosclerosis / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D001688:Biological Products / Q000008:administration & dosage / Q000737:chemistry; D002791:Cholesterol, Dietary / Q000494:pharmacology; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D009177:Mycoplasma pneumoniae / Q000187:drug effects* / Q000472:pathogenicity; D009439:Neuraminidase / Q000008:administration & dosage* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D058226:Plaque, Atherosclerotic / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D011817:Rabbits
D001688:Biological Products; D002791:Cholesterol, Dietary; D006023:Glycoproteins; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C069715:trans-sialidase; D009439:Neuraminidase