Binding of TEM-1 beta-lactamase to beta-lactam antibiotics by frontal affinity chromatography.
TEM-1 beta-lactamases can accurately catalyze the hydrolysis of the beta-lactam rings in beta-lactam antibiotics, which make beta-lactam antibiotics lose its activity, and the prerequisite for the hydrolysis procedure in the binding interaction of TEM-1 beta-lactamases with beta-lactam antibiotics is the beta-lactam rings in beta-lactam antibiotics. Therefore, the binding of TEM-1 beta-lactamase to three beta-lactam antibiotics including penicillin G, cefalexin as well as cefoxitin was explored here by frontal affinity chromatography in combination with fluorescence spectra, adsorption and thermodynamic data in the temperature range of 278-288K under simulated physiological conditions. The results showed that all the binding of TEM-1 beta-lactamase to the three antibiotics were spontaneously exothermic processes with the binding constants of 8.718×103, 6.624×103and 2.244×103(mol/L), respectively at 288K. All the TEM-1 beta-lactamases were immobilized on the surface of the stationary phase in the mode of monolayer and there existed only one type of binding sites on them. Each TEM-1 beta-lactamase bound with only one beta-lactam antibiotic and hydrogen bond interaction and Van der Waals force were the main forces between them. This work provided an insight into the binding interactions between TEM-1 beta-lactamases and beta-lactam antibiotics, which may be beneficial for the designing and developing of new substrates resistant to TEM-1 beta-lactamases.
College of Life Science, Northwest University, Xi'an 710069, PR China.
Xiu
X
Chen
College of Life Science, Northwest University, Xi'an 710069, PR China.
Yuhua
Y
Li
College of Life Science, Northwest University, Xi'an 710069, PR China.
Yan
Y
Zhang
College of Life Science, Northwest University, Xi'an 710069, PR China; Weapon Industry 521 Hospital, Xi'an 710065, PR China.
Jianting
J
Yang
College of Life Science, Northwest University, Xi'an 710069, PR China. Electronic address: bianliujiao@sohu.com.
Liujiao
L
Bian
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002440:Cefoxitin / Q000378:metabolism; D002506:Cephalexin / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000378:metabolism; D010400:Penicillin G / Q000378:metabolism; D011485:Protein Binding; D013816:Thermodynamics; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D004800:Enzymes, Immobilized; D047090:beta-Lactams; D002440:Cefoxitin; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; D002506:Cephalexin; D010400:Penicillin G
Beta-lactam antibiotics; Binding; Frontal affinity chromatography; TEM-1 beta-lactamase