Rosmarinic acid plays a protective role in the embryogenesis of zebrafish exposed to food colours through its influence on aurora kinase A level.
OBJECTIVE
To evaluate the protective nature of the rosmarinic acid from Sphaeranthus amaranthoides during zebra fish embryogenesis.


METHODS
Rosmarinic acid was isolated from the S. amaranthoides. An accurate, sensitive and simple LC-MS analysis was performed to determine the rosmarinic acid from S. amaranthoides. In the present study, zebrafish embryos were exposed to crimson red and sunset yellow at a concentration of 0.1 and 0.5mg/l and the effect of these food colours on the levels of aurora kinase A was studied individually.


RESULTS
Aurora kinase A levels are crucial for embryogenesis in zebrafish which is used as model in this study. The decrease of aurora kinase A levels in food colour treated embryos influences the embryogenesis, resulting in short and bent trunk leading to cell death and growth retardation. Elevated levels of aurora kinase A in rosmarinic acid treated groups can be attributed to the restoration of normal growth in zebra fish embryos with well developed brain and eyes. Further insilico docking studies were carried out and target was identified as rosmarinic acid. From the docking studies the docking poses and binding energy confirms that aurora kinase A is the target for rosmarinic acid.


CONCLUSIONS
Rosmarinic acid was found to play a protective role in the embryogenesis of zebra fish exposed to food colours (crimson red and sunset yellow) through its influence on aurora kinase A levels.
Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, India. Electronic address: lokiswarna@gmail.com.
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Swarnalatha
Centre for Drug Discovery and Development, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, India. Electronic address: jerrine.jj@gmail.com.
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Jerrine Joseph
Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, India. Electronic address: jayakrish.t@gmail.com.
Tippabathani
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Jayakrishna
D000818:Animals; D064096:Aurora Kinase A / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D003116:Color; D053630:Depsides / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D000097123:Rosmarinic Acid
D064096:Aurora Kinase A; D002934:Cinnamates; D053630:Depsides; D020011:Protective Agents; D000097123:Rosmarinic Acid
Aurora kianse A; Column chromatography; Discovery Studio; Docking; Rosmarinic acid