Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer.
Temporary single-cell coating is a useful tool for cell processing, allowing manipulation of cells to prevent cell attachment and agglomeration, before re-establishing normal cell function. In this work, a speckled coating method using a known polycation [poly(l-lysine), PLL] is described to induce cell surface electrostatic charges on three different cell types, namely, two bone cancer cell lines and fibroblasts. The morphology of the PLL speckled coating on the cell surface, internalization and metabolization of the polymer, and prevention of cellular aggregations are reported. Polymer concentration was found to be the key parameter controlling both capsule morphology and cell health. This approach allows a temporary cell coating over the course of 1-2 h, with cells exhibiting phenotypically normal behavior after ingesting and metabolizing the polymer. The process offers a fast and efficient alternative to aid single-cell manipulation for bioprocessing applications. Preliminary work on the application of PLL speckled cell coating in enabling reliable bioprinting is also presented.
School of Mechanical and Systems Engineering, Newcastle University , Newcastle Upon Tyne NE1 7RU, U.K.
Ricardo D C
http://orcid.org/0000-0003-1591-0499
RDC
Ribeiro
Deepali
D
Pal
David
D
Jamieson
Kenneth S
KS
Rankin
School of Mechanical and Systems Engineering, Newcastle University , Newcastle Upon Tyne NE1 7RU, U.K.
Matthew
M
Benning
School of Mechanical and Systems Engineering, Newcastle University , Newcastle Upon Tyne NE1 7RU, U.K.
Kenneth W
KW
Dalgarno
School of Mechanical and Systems Engineering, Newcastle University , Newcastle Upon Tyne NE1 7RU, U.K.
Ana M
http://orcid.org/0000-0002-7728-1619
AM
Ferreira
D002214:Capsules; D002412:Cations; D002460:Cell Line; D011107:Polylysine; D011108:Polymers / Q000737:chemistry*
D002214:Capsules; D002412:Cations; D011108:Polymers; D011107:Polylysine
bioprinting; bioprocessing; cellular uptake; coating; polycation; single cells; temporary