Murine hepatocytes DNA changes as an assessment of the immunogenicity of potential anti-schistosomal vaccines experimentally.
Hepatic affection by granulomatous inflammation in schistosomiasis suggested that a potential anti-pathology vaccine could be generated based on limiting the presence of hazardous hepatocytes induced apoptosis and caused reduction of granulomas number and size . So, this work is concerned with experimental assessment of the efficacy of differentSchistosoma mansoniantigens (SEA, SWAP and combined SEA and SWAP) on murine liver after challenge bySchistosomainfection, histopathological, histochemical and molecular investigations were performed on sixty male laboratory bred Swiss Albino mice. A schedule of vaccination and challenge infection was followed and performed on 6 mice groups (each of ten); control normal (G1), control infected (G2), adjuvant received then infected (G3), SEA + adj. received then infected (G4), SWAP + adj. received then infected (G5) and SEA + SWAP + adj. received then infected (G6).Animals were euthanized 10 weeks post infection.Vaccination efficacy was assessed by histopathological, histochemical and molecular studies on murine hepatic tissues.Results showed that:The combined (SEA + SWAP) antigens were better in reducing the number and diameter of the hepatic granulomas, with more protection of the hepatocytes DNA, in addition to more decrease of hepatocytes induced apoptosis and fragmentation as demonstrated by molecular assay.
Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Samia E
SE
Etewa
Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Mohamed H A
MHA
Hegab
Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Ashraf S
AS
Metwally
Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Somia H
SH
Abd Allah
Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Sally M
SM
Shalaby
Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Amal S
AS
El-Shal
Histology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Mohamed
M
Baredy
Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Mahmoud A
MA
El Shafey
Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Howayda S F
HSF
Moawad
Fragmentation; Hepatocytes DNA; Induced apoptosis; Molecular study; Schistosoma mansoni