9-cis-Neoxanthin in Light Harvesting Complexes of Photosystem II Regulates the Binding of Violaxanthin and Xanthophyll Cycle.
The light-harvesting chlorophylla/bcomplex of photosystem II (LHCII) is able to switch to multiple functions under different light conditions (i.e. harvesting solar energy for photosynthesis and dissipating excess excitation energy for photoprotection). The role of the different carotenoids bound to LHCII in regulating the structure and function of the complex is a long-lasting question in photosynthesis research. 9-cis-Neoxanthin (Nx) is one of the important carotenoids, which can only be found in the LHCIIs. High-resolution structural analysis of LHCII shows that Nx is located between different monomeric LHCIIs, with one side protruding into the lipid membrane. In this study, the various functional significances of this unique feature of Nx binding in LHCII are studied with the in vitro reconstituted LHCIIs both with and without Nx and the native complexes isolated either from wild-type Arabidopsis (Arabidopsis thaliana) or from its mutantaba4-3lacking Nx Our results reveal that the binding of Nx affects the binding affinity of violaxanthin (Vx) to LHCII significantly. In the absence of Nx, Vx has a much higher binding affinity to trimeric LHCII. The strong coordination between Nx and Vx at the interfaces of adjacent monomers of LHCII plays an important role both in operating the xanthophyll cycle and in the transient modulation of nonphotochemical quenching.
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (K.W., W.T., C.L., Y.R., C.Y.).|University of the Chinese Academy of Sciences, Beijing 100049, China (K.W.); and.|Key Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China (Z.G.).
Ke
K
Wang
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (K.W., W.T., C.L., Y.R., C.Y.).|University of the Chinese Academy of Sciences, Beijing 100049, China (K.W.); and.|Key Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China (Z.G.).
Wenfeng
W
Tu
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (K.W., W.T., C.L., Y.R., C.Y.).|University of the Chinese Academy of Sciences, Beijing 100049, China (K.W.); and.|Key Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China (Z.G.).
Cheng
C
Liu
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (K.W., W.T., C.L., Y.R., C.Y.).|University of the Chinese Academy of Sciences, Beijing 100049, China (K.W.); and.|Key Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China (Z.G.).
Yan
Y
Rao
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (K.W., W.T., C.L., Y.R., C.Y.).|University of the Chinese Academy of Sciences, Beijing 100049, China (K.W.); and.|Key Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China (Z.G.).
Zhimin
Z
Gao
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (K.W., W.T., C.L., Y.R., C.Y.); yangch@ibcas.ac.cn.|University of the Chinese Academy of Sciences, Beijing 100049, China (K.W.); and yangch@ibcas.ac.cn.|Key Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China (Z.G.) yangch@ibcas.ac.cn.
Chunhong
http://orcid.org/0000-0003-1087-8135
C
Yang
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D009154:Mutation; D010788:Photosynthesis / Q000235:genetics / Q000528:radiation effects; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D011485:Protein Binding / Q000528:radiation effects; D013053:Spectrophotometry; D024341:Xanthophylls / Q000378:metabolism*
D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D024341:Xanthophylls; D002734:Chlorophyll; C005613:violaxanthin; C011947:neoxanthin