Evaluation of Phenolic Compounds and Antioxidant and Antimicrobial Activities of Some Common Herbs.
The study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion (Allium cepa), garlic (Allium sativum), mint (Mentha spicata), thyme (Thymus vulgaris), oak (Quercus), aloe vera (Aloe barbadensisMiller), and ginger (Zingiber officinale). All extracts showed a wide range of total phenolic contents, that is, 4.96 to 98.37 mg/100 g gallic acid equivalents, and total flavonoid contents, that is, 0.41 to 17.64 mg/100 g catechin equivalents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system, and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Different extracts inhibited oxidation of linoleic acid by 16.6-84.2% while DPPH radical scavenging activity (IC50values) ranged from 17.8% to 79.1μg/mL. Reducing power at 10 mg/mL extract concentration ranged from 0.11 to 0.84 nm. Furthermore the extracts of these medicinal herbs in 80% methanol, 80% ethanol, 80% acetone, and 100% water were screened for antimicrobial activity by disc diffusion method against selected bacterial strains,Staphylococcus aureus,Escherichia coli,Bacillus subtilis, andPasteurella multocida, and fungal strains,Aspergillus niger,Aspergillus flavus, Rhizopus solani, andAlternaria alternata. The extracts show better antimicrobial activity against bacterial strains as compared to fungal strains. Results of various assays were analyzed statistically by applying appropriate statistical methods.
Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
Muhammad
M
Abdul Qadir
Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
Syeda Kiran
0000-0002-7041-5776
SK
Shahzadi
Department of Chemistry, Minhaj University, Lahore 54590, Pakistan.
Asad
A
Bashir
Department of Chemistry, Minhaj University, Lahore 54590, Pakistan.
Adil
A
Munir
Institute of Chemistry, University of the Punjab, Lahore 54590, Pakistan.
Shabnam
0000-0002-1786-5559
S
Shahzad