Port-site infections by nontuberculous mycobacterium: A retrospective clinico-microbiological study.
Port-site infection (PSI) is a prevailing, chronic, nagging, treatment refractory complication of laparoscopic surgery (LS). It neutralizes the advantages of minimally invasive surgery and increases morbidity, treatment cost of patient, leading to loss of confidence on operating surgeon. PSIs are preventable with appropriate preoperative, intraoperative, and postoperative measures. Atypical mycobacterium is most commonly associated with nonhealing postlaparoscopic wound infections, causing outbreaks or sporadic cases worldwide.



We retrospectively studied the occurrence of nontuberculous mycobacterium (NTM) from PSIs following LS that did not respond to antibiotics used for pyogenic infections and having sterile routine aerobic cultures and their antimicrobial susceptibility pattern to guide proper management.



The study was done in a tertiary care hospital of Eastern India over a 1-year period which included PSI cases with delayed onset not responding to antibiotics, following different types of LS. Pus/discharge from 32 patients was collected and examined for isolation and identification of the causative agents. Gram stain and Ziehl-Neelsen staining methods were used for direct examination. Culture media included blood agar, Robertson's cooked meat broth, MacConkey agar, and Lowenstein-Jensen medium. Isolates from the cases were identified using biochemical tests or molecular methods and studied the antimicrobial susceptibility pattern by the standard microbiologic procedures.



Mycobacterium abscessus (13) and Mycobacterium fortuitum (2) were isolated from 15 serosanguinous drainage obtained from 32 cases by routine microbiological techniques. All isolates analyzed for antimicrobial susceptibility pattern were highly sensitive to clarithromycin (93.3%), amikacin (93.3%), and imipenem (80%) but were variable to ciprofloxacin, ofloxacin, and linezolid.



Our present study shows frequent association of NTM with laparoscopic port-site nonhealing chronic infection or wound dehiscence. Although direct microscopy can give us a clue to diagnosis, culture isolation is required for speciation and antimicrobial susceptibility testing, which helps formulate therapeutic regimen.
Department of Microbiology, ESI-PGIMSR and ESIC Medical College, Kolkata, West Bengal, India.
Roumi
R
Ghosh
Department of General Surgery, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.
Soumen
S
Das
Department of Microbiology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.
Asmita
A
De
Department of General Surgery, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.
Harish
H
Kela
Department of General Surgery, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.
Makhan Lal
ML
Saha
Department of Microbiology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.
Prasanta Kumar
PK
Maiti
D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002939:Ciprofloxacin / Q000494:pharmacology; D005260:Female; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007194:India / Q000453:epidemiology; D010535:Laparoscopy / Q000009:adverse effects; D000069349:Linezolid / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology / Q000382:microbiology*; D000073358:Mycobacterium abscessus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D019907:Mycobacterium fortuitum / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification*; D012189:Retrospective Studies; D013530:Surgical Wound Infection / Q000453:epidemiology / Q000382:microbiology*; D062606:Tertiary Care Centers; D055815:Young Adult
D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D015378:Imipenem; D000069349:Linezolid