Population genetic structure and comparative diversity of smallmouth bass Micropterus dolomieu: congruent patterns from two genomes.
Genetic diversity and divergence patterns of smallmouth bass Micropterus dolomieu spawning groups are analysed across its northern native range with mtDNA cytochrome b gene sequences and eight unlinked nuclear DNA microsatellite loci. Results reveal high levels of genetic variability and significant differences in allelic representation among populations (mtDNA: mean ± s.e., HD= 0·50 ± 0·06, mean ± s.e., θST= 0·41 ± 0·02 and microsatellites: mean ± s.e. HO= 0·46 ± 0·03, mean ± s.e. θST= 0·25 ± 0·01). The distributions of 28 variant mtDNA haplotypes, which differ by an average of 3·94 nucleotides (range = 1-8), denote divergent representation among geographic areas. Microsatellite data support nine primary population groups, whose high self-assignment probabilities likewise display marked divergence. Genetic patterns demonstrate: (1) high genetic diversity in both genomes, (2) significant divergence among populations, probably resulting from natal site homing and low lifetime migration, (3) support for three post-glacial refugia that variously contributed to the current northern populations, which remain evident today despite waterway connectivity and (4) a weak yet significant genetic isolation by geographic distance pattern, indicating that other processes affect the differences among populations, such as territoriality and site fidelity.
Great Lakes Genetics/Genomics Laboratory, Department of Environmental Sciences, University of Toledo, Toledo, OH, 43606, U.S.A.|NOAA Pacific Marine Environmental Laboratory (PMEL), 7600 Sand Point Way NE, Seattle, WA, 98115, U.S.A.
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CA
Stepien
Great Lakes Genetics/Genomics Laboratory, Department of Environmental Sciences, University of Toledo, Toledo, OH, 43606, U.S.A.
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Karsiotis
Great Lakes Genetics/Genomics Laboratory, Department of Environmental Sciences, University of Toledo, Toledo, OH, 43606, U.S.A.
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TJ
Sullivan
Great Lakes Genetics/Genomics Laboratory, Department of Environmental Sciences, University of Toledo, Toledo, OH, 43606, U.S.A.
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Klymus
D000483:Alleles; D000818:Animals; D001492:Bass / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D016678:Genome; D006239:Haplotypes; D018895:Microsatellite Repeats
D004272:DNA, Mitochondrial
Great Lakes; Micropterus dolomieu; cytochrome b; microsatellites; phylogeography; smallmouth bass