Natural product inhibitors of carbonic anhydrase I and II isoenzymes: osajin and pomiferin.
The aim of this study is to purify carbonic anhydrase I and II isoenzymes from human erythrocyte, isolate two natural products osajin (OSJ) and pomiferin (PMF) from Maclura pomifera fruits, and evaluate the in vitro effect of these natural metabolites on these isoenzymes. These natural products may be used as starting points for drug discovery (like drugs used in several therapeutic applications, including antiglaucoma activity). For the purification procedure, the Sepharose-4B-l-tyrosine-sulphonamide affinity chromatography was used. Column chromatography and thin layer chromatography methods were used for isolation of OSJ and PMF from M. pomifera fruits and their chemical structures were elucidated by IR, 1D, and 2D NMR methods. We compared inhibitory effects of these natural products with inhibitory effects of phenolic compounds and found that these products demonstrated average inhibition effects. We thought that this study will give inspiration to scientists interested in this issue.
a Faculty of Pharmacy, Department of Biochemistry , Erzincan University , Erzincan , Turkey.
Esra
E
Dilek
b Faculty of Veterinary, Department of Biochemistry , Ataturk University , Erzurum , Turkey.
Hüseyin Serkan
HS
Erol
c Faculty of Science, Department of Chemistry , Kilis 7 Aralık University , Kilis , Turkey.
Ahmet
A
Cakir
d Animal Production Schools , Bozok University , Yozgat , Turkey.
Murat
http://orcid.org/0000-0002-1156-0068
M
Koc
b Faculty of Veterinary, Department of Biochemistry , Ataturk University , Erzurum , Turkey.
Mesut Bünyami
MB
Halici
D001578:Benzopyrans / Q000737:chemistry*; D001688:Biological Products / Q000494:pharmacology*; D024401:Carbonic Anhydrase I / Q000037:antagonists & inhibitors*; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D007529:Isoflavones / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
D001578:Benzopyrans; D001688:Biological Products; D002257:Carbonic Anhydrase Inhibitors; D007529:Isoflavones; D010936:Plant Extracts; C474837:pomiferin; C485800:osajin; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II
Carbonic anhydrase I; carbonic anhydrase II; inhibition; osajin; pomiferin