Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques.
The development of techniques and methods for allergen purification is essential for diagnosis and the development of safe immunotherapeutic agents. The most common purification techniques include chromatographic methodologies. In this chapter, we review and describe the details of the methodologies of using ion-exchange, gel-filtration, and affinity chromatography to purify two well-known panallergens, profilin and parvalbumin.
Research and Development Department, Inmunotek S.L., Madrid, Spain.
Barbara
B
Cases
Department of Immunology, IIS Fundación Jiménez Diaz-UAM, Madrid, Spain.
Carlos
C
Pastor-Vargas
Institute for Applied Molecular Medicine (IMMA), School of Medicine, Universidad CEU San Pablo, Madrid, Spain. marina.perezgordo@ceu.es.
Marina
M
Perez-Gordo
D000485:Allergens / Q000737:chemistry* / Q000302:isolation & purification*; D001688:Biological Products / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans
D000485:Allergens; D001688:Biological Products
Allergen extraction; Allergen purification; Allergy; Chromatographic techniques; Chromatography; Parvalbumin; Profilin