In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide.
This study was designed to determine whether nano-sized ZnO has the potential to cause acute cutaneous irritation using cultured HaCaT keratinocytes and a human skin equivalent as in vitro models, compared to non-nanomaterials. Commercial nano ZnO with different sizes (50 nm and 100 nm) was characterized by dynamic light scattering (DLS) and microscopy (SEM) in different media. Nano ZnO reduced the cell viability of HaCaT in a dose-dependent and time-dependent manner, in a similar way to macro ZnO. However, the 3D-epidermis model revealed no irritation at 1 mg/mL after 24 h of exposure. In conclusion, nano-sized ZnO does not irritate skin, in a similar manner to non-nano ZnO.
Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Av. Joan XXIII 27-31, Barcelona 08028, Spain. mpvinardellmh@ub.edu.
Maria Pilar
MP
Vinardell
Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Av. Joan XXIII 27-31, Barcelona 08028, Spain. hectorllanasmarco@gmail.com.
Hector
H
Llanas
Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Av. Joan XXIII 27-31, Barcelona 08028, Spain. laura.marfa.89@gmail.com.
Laura
L
Marics
Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Av. Joan XXIII 27-31, Barcelona 08028, Spain. montsemitjans@ub.edu.
Montserrat
M
Mitjans
3D-epidermal model; cytotoxicity; keratinocytes; skin irritation; zinc oxide