[The influence and mechanisms of lysine-specific demethylase 1 (LSD1) on invasion and metastasis of colon cancer cells].
Objective:To investigate the effect and molecular mechanisms of LSD1 on proliferation and metastasis of colon cancer.Methods:The influence of down-regulated LSD1 expression on proliferation, invasion and apoptosis of colon cancer cells were detected by transwell invasion assay, cell proliferation assay and apoptosis assay, respectively.Results:Three independent siRNAs targeting LSD1 (siRNA-1554, siRNA-705, and siRNA-1973) were transfected to SW620 cells to detect gene-silencing efficiency, and the result showed that the knockdown effect of siRNA-705 were better than the other two siRNAs at both mRNA and protein levels. Using siRNA-705 and pargyline (2.5 mmol/L), we performed transwell invasion assay, cell proliferation assay and apoptosis assay in SW620 cell lines, and found the significant suppression of invasion and growth. Cell apoptosis were induced by siRNA and pargyline (P<0.05). Interestingly, up-regulation of E-cadherin and down-regulation of N-cadherin were observed after treated with siRNA-705 and pargyline for 72 hours.Conclusion:Inhibition of LSD1 could impair proliferation and invasiveness, and induce apoptosis of colon cancer cellsin vitro. It leads to up-regulation of E-cadherin and down-regulation of N-cadherin. All of above may play important roles in invasion and metastasis of colon cancer.
Department of Gastrointestinal Surgery, Gui Zhou Provincial People's Hospital, Guiyang 550002, China.
J
J
Ding
Z M
ZM
Zhang
Q
Q
Dong
R
R
Mi
K S
KS
Xu
X F
XF
Yang
G Q
GQ
Liao
D015703:Antigens, CD; D017209:Apoptosis; D015820:Cadherins; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms; D015536:Down-Regulation; D056466:Histone Demethylases; D006801:Humans; D008239:Lysine; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D034741:RNA, Small Interfering; D014162:Transfection
D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D034741:RNA, Small Interfering; D056466:Histone Demethylases; C495195:KDM1A protein, human; D008239:Lysine
E-cadherin; Metastasis; N-cadherin; Rectal neoplasms; lysine-specific demethylase 1