Does systemic administration of casein phosphopeptides affect orthodontic movement and root resorption in rats?
OBJECTIVE
To assess the potential effects of casein phosphopeptides (CPPs) on orthodontically induced iatrogenic root resorption (OIIRR) and orthodontic teeth movement.


METHODS
Forty Wistar rats (aged 11 weeks) were randomly divided into experimental group (EG; n = 20) that received a diet supplemented with CPP and control group (CG; n = 20) devoid of diet supplement. A 150 g force was applied using nickel titanium (NiTi) coil that was bonded on maxillary incisors and extended unilaterally to a maxillary first molar. At Day 28, animals in both groups were euthanized. Volumetric assessment of root resorption craters and linear measurement of maxillary first molars movement were blindly examined using a micro-computed tomography scan.


RESULTS
Nine rats were excluded from the experiment due to loss during general anesthesia or appliances' failure. Intra-operator reproducibility was high in both volumetric and linear measurements, 92.8 per cent and 98.5-97.6 per cent, respectively. The results reveal that dietary CPP has statistically insignificant effect on the overall OIIRR and orthodontic movement.


CONCLUSIONS
CPP seems to have statistically insignificant effect on the volume of OIIRR and orthodontic movement in rats. A long-term study with larger sample size using a different concentration of CPP is required to clarify the dentoalveolar effect of CPP.
Discipline of Orthodontics, Faculty of Dentistry, University of Sydney, New South Wales, Australia.
Lachlan
L
Crowther
Department of Orthodontics, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Gang
G
Shen
Discipline of Orthodontics, Faculty of Dentistry, University of Sydney, New South Wales, Australia.|Oxford University and UCL Hospitals NHS Foundation Trust, England, UK.
Mohammed
M
Almuzian
Electronic Microscopic Unit, University of Sydney, New South Wales.
Allan
A
Jones
Surgical and Orthopaedic Research Laboratories, University of New South Wales, Prince of Wales Hospital, Sydney.
William
W
Walsh
Surgical and Orthopaedic Research Laboratories, University of New South Wales, Prince of Wales Hospital, Sydney.
Rema
R
Oliver
Department of Statistics, Macquarie University, Sydney, New South Wales, Australia.
Peter
P
Petocz
Discipline of Orthodontics, Faculty of Dentistry, University of Sydney, New South Wales, Australia.
Nour E
NE
Tarraf
Discipline of Orthodontics, Faculty of Dentistry, University of Sydney, New South Wales, Australia.
M Ali
MA
Darendeliler
D000497:Alloys; D000818:Animals; D002364:Caseins / Q000627:therapeutic use*; D003722:Dental Alloys; D019587:Dietary Supplements; D007180:Incisor / Q000503:physiopathology; D008297:Male; D008963:Molar / Q000503:physiopathology; D010748:Phosphopeptides / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012391:Root Resorption / Q000209:etiology / Q000517:prevention & control*; D014087:Tooth Movement Techniques / Q000009:adverse effects* / Q000379:methods; D055114:X-Ray Microtomography / Q000379:methods
D000497:Alloys; D002364:Caseins; D003722:Dental Alloys; D010748:Phosphopeptides; C013616:nitinol