Limnothrix sp. KO05: A newly characterized cyanobacterial biosorbent for cadmium removal: the enzymatic and non-enzymatic antioxidant reactions to cadmium toxicity.
In this study, we isolated five indigenous cyanobacterial strains from different aqueous environments, with heavy metals contamination, in East Azerbaijan Province (northwest portion of Iran). A strain was identified by morphological and 16S rRNA sequence analysis as Limnothrix sp. KO05 and selected for further studies as having the greatest potential for cadmium uptake. Scanning electron microscopy (SEM) demonstrated cyanobacterium Limnothrix sp. KO05 forms filamentous structures and is straight or curved to some extent. The utmost biosorption capacity was found to be 82.18±1.22mgg-1at a Cd (II) concentration level of 150mgL-1. Langmuir adsorption isotherm indicated a better fit to the experimental data. Response surface methodology (RSM) on the basis of four independent variables and the predicted maximum biosorption efficiency was 98.7% under the optimum condition. FT-IR spectroscopy profile of the Cd treated sample as demonstrated in confirmation of the benefits of various functional groups of proteins and polysaccharides of cyanobacterial biomass, involved in surface binding of Cd. The determination of catalase (CAT) activity in strain KO05 exposed to Cd (II) concentrations of 2, 5 and 10mgL-1showed an increase in enzyme activity after 24h exposure compared to unexposed cells. Correspondingly, CAT activity showed a significant decrease after 48h of treatment with Cd (II) concentrations of 5 and 10mgL-1. CAT activity was decreased significantly at all concentrations within 72h after exposure to Cd. On the contrary, while ascorbate peroxidase (APX) gave the expected lower activity compared to the CAT within 24h after Cd treatment, its activity lasted up to 72h. Limnothrix sp. KO05 cells treated with 5 and 10mgL-1Cd (II) over 72h exposure showed a reduction in chlorophyll a contents compared to the controls. However, following exposure to Cd, chlorophyll a and carotenoid contents is reduced and after overcoming stress and deployment of an adaptation mechanism, the amounts of these pigments is gradually increased in the cells. The reduction was slower for chlorophyll a pigment compared to carotenoids that may be an indication of the physiological importance of chlorophyll pigment for the phtosynthetic cells. Results related to lipid peroxidation in Limnothrix sp. KO05 represent a significant increase of MDA in the first 24h after exposure to the different concentrations of Cd (2, 5 and 10mgL-1). However, the MDA levels were decreased over time and no significant difference attained after 72h exposure to Cd concentrations of 2 and 10mgL-1compared to control.
Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
Omid
O
Haghighi
Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
Shahab
S
Shahryari
Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
Mojgan
M
Ebadi
Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
Sima
S
Modiri
Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
Hossein Shahbani
HS
Zahiri
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Hadi
H
Maleki
Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran. Electronic address: Akbari@nigeb.ac.ir.
Kambiz Akbari
KA
Noghabi
D000975:Antioxidants / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001692:Biological Transport; D018533:Biomass; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D000458:Cyanobacteria / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D004784:Environmental Monitoring; D007492:Iran; D008962:Models, Theoretical; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D000975:Antioxidants; D014874:Water Pollutants, Chemical; D002104:Cadmium
Ascorbate proxidase (APX); Biosorption; Cadmium; Catalase (CAT); Kinetic/thermodynamic; Limnothrix sp. KO05; Malondialdehyde (MDA)