Culture of non-typeable Haemophilus influenzae from the nasopharynx: Not all media are equal.
The efficacy of chocolate agar, versus bacitracin, vancomycin, clindamycin, chocolate agar (BVCCA) for the isolation of non-typeable Haemophilus influenzae (NTHi) from nasopharyngeal swabs was determined. BVCCA cultured NTHi from 97.3% of NTHi-positive swabs, compared to 87.1% for chocolate agar. To maximise culture sensitivity, the use of both media is recommended.
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia. Electronic address: tegan.harris@menzies.edu.au.
Tegan M
TM
Harris
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia. Electronic address: angela.rumaseb@menzies.edu.au.
Angela
A
Rumaseb
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia. Electronic address: jemima.beissbarth@menzies.edu.au.
Jemima
J
Beissbarth
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia. Electronic address: federica.barzi@menzies.edu.au.
Federica
F
Barzi
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia. Electronic address: amanda.leach@menzies.edu.au.
Amanda J
AJ
Leach
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia; School of Medicine, Griffith University, Gold Coast, Queensland, Australia. Electronic address: heidi.smith-vaughan@menzies.edu.au.
Heidi C
HC
Smith-Vaughan
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D006192:Haemophilus Infections / Q000382:microbiology; D006193:Haemophilus influenzae / Q000166:cytology* / Q000187:drug effects / Q000302:isolation & purification; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D003470:Culture Media
Agar culture medium sensitivity; Bacitracin, vancomycin, clindamycin, chocolate agar (BVCCA); Chocolate agar; Nasopharynx; Non-typeable Haemophilus influenzae (NTHi)