Correlations betweenACEsingle nucleotide polymorphisms and prognosis of patients with septic shock.
The aim of the present study is to investigate association between septic shock (SS) and angiotensin I-converting enzyme (ACE) single nucleotide polymorphisms (SNPs). From October 2009 to December 2016, 238 SS patients and 242 healthy individuals were selected for our study. ACE activity was detected,ACErs4291 and rs4646994 polymorphisms were detected using PCR-restriction fragment length polymorphism (PCR-RFLP). The Kaplan-Meier survival curve was employed to evaluate the association betweenACESNPs and patients' survival and univariate and multivariate analyses to estimate risk factors for SS. ACE activity in the case group was increased in comparison with the control group. Allele and genotype frequencies of rs4291 and rs4646994 were different between the case and control groups. The TT genotype frequency of the rs4291 polymorphisms and the DD genotype of the rs4646994 polymorphisms of the case group were higher than those in the control group. The AT and TT genotypes indicated a significant elevation of ACE activity than the AA genotype, while a significant decline was found in the DI and II genotypes in comparison with the DI genotype. Patients with TT or DD genotypes had increased fatality rate within 7 and 30 days when compared with those with non-TT or non-DD genotypes. Lower sepsis-related organ failure assessment (SOFA) scores, rs4291, serum ACE and rs4646994 were all considered as risky factors for SS patients. The study demonstrates that TT genotype of rs4291 or DD genotype of rs4646994 may be indicative of a higher risk of SS and a poorer prognosis in SS patients.
Life Sciences School of Lanzhou University, Lanzhou 730000, P.R. China.|Department of General Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, P.R. China.
Xin-Man
XM
Dou
Department of General Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, P.R. China.|Key Laboratory of Digestive System Tumors of Gansu Province, Lanzhou 730030, P.R. China.
Hui-Juan
HJ
Cheng
Department of General Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, P.R. China.
Ling
L
Meng
Nursing School of Lanzhou University, Lanzhou 730000, P.R. China.
Lin-Lin
LL
Zhou
Nursing School of Lanzhou University, Lanzhou 730000, P.R. China.
Yi-Hong
YH
Ke
Department of Intensive Care Unit, First Hospital of Lanzhou University, Lanzhou 730030, P.R. China.
Li-Ping
LP
Liu
Life Sciences School of Lanzhou University, Lanzhou 730000, P.R. China drliyumin@163.com.|Department of General Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, P.R. China.|Key Laboratory of Digestive System Tumors of Gansu Province, Lanzhou 730030, P.R. China.
Yu-Min
YM
Li
D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012307:Risk Factors; D012772:Shock, Septic / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D016019:Survival Analysis
D007703:Peptidyl-Dipeptidase A
Angiotensin I-converting enzyme; Prognosis; Septic shock; Single nucleotide polymorphisms