Ionic liquid-regenerated macroporous cellulose monolith: Fabrication, characterization and its protein chromatography.
Macroporous cellulose monolith as chromatographic support was successfully fabricated from an ionic liquid dissolved cellulose solution by an emulsification method and followed by the cross-linking reaction and DEAE modification. With the physical characterization, the cellulose monolith featured by both the interconnected macropores in range of 0.5-2.5μm and the diffusion pores centered at about 10nm. Given the bimodal pore system, the monolith possessed the specific surface area of 36.4m2g-1and the column permeability of about 7.45×10-14m2. After the DEAE modification, the anion cellulose monolith was evaluated for its chromatography performances. It demonstrated that the static and dynamic adsorption capacity of BSA reached about 66.7mgmL-1and 43.9mgmL-1at 10% breakthrough point, respectively. The results were comparable to other chromatographic adsorbent. In addition, the proteins mixture with different pI was well separated at high flow velocity (611.0cmh-1) and high protein recovery (over 97%), proving the macroporous cellulose monolith had excellent separation performance. In this way, the prepared cellulose monolith with bimodal pores system is expected for the potential application in high-speed chromatography.
Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: kfdu@scu.edu.cn.
Kaifeng
K
Du
D000327:Adsorption; D002482:Cellulose / Q000138:chemical synthesis / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D004058:Diffusion; D052578:Ionic Liquids / Q000737:chemistry*; D007477:Ions; D010539:Permeability; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
D052578:Ionic Liquids; D007477:Ions; D011506:Proteins; D002482:Cellulose
Cellulose; Emulsification; Ionic liquid; Porous monolith; Protein chromatography