Moderate selenium dosing inhibited chromium (VI) toxicity in chicken liver.
This study aimed to clarify the effect of selenium (Se) on chromium (VI) [Cr(VI)]-induced damage in chicken liver. A total of 105 chickens were randomly divided into seven groups of 15. Group I received deionized water; group II received Cr(VI) (7.83 mg/kg/d) alone; and other groups orally received both Cr(VI) (7.83 mg/kg/d) and Se of different doses (0.14, 0.29, 0.57, 1.14, and 2.28 mg/kg/d). The levels of superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), Ca2+-ATPase, and mitochondrial membrane potential (MMP) were measured. Results showed that Cr(VI) increased MDA content and decreased GSH content, T-SOD activity, Ca2+-ATPase activity, and MMP level. Meanwhile, Se co-treatment (0.14, 0.29, and 0.57 mg/kg/d) increased the viability of the above indicators compared with Cr(VI)-treatment alone. In addition, histopathologic examination revealed that Cr(VI) can cause liver damage, whereas Se supplementation of moderate dose inhibited this damage. This study confirmed that Se exerted protective effect against Cr(VI)-induced liver damage.
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Yang
Y
Wang
Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Yongxia
Y
Liu
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Huiyu
H
Wan
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Yiran
Y
Zhu
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Peng
P
Chen
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Pan
P
Hao
Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Ziqiang
Z
Cheng
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong, 271018, People's Republic of China.
Jianzhu
J
Liu
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002645:Chickens; D002857:Chromium / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D012643:Selenium / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D002857:Chromium; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium
chromium (VI); histopathological studies; liver damage; oxidative stress; selenium