Chloroplast microsatellite markers forPseudotaxus chieniideveloped from the whole chloroplast genome ofTaxus chinensisvar.mairei(Taxaceae).
OBJECTIVE
Pseudotaxus chienii(Taxaceae) is an old rare species endemic to China that has adapted well to ecological heterogeneity with high genetic diversity in its nuclear genome. However, the genetic variation in its chloroplast genome is unknown.


RESULTS
Eighteen chloroplast microsatellite markers (cpSSRs) were developed from the whole chloroplast genome ofTaxus chinensisvar.maireiand successfully amplified in fourP. chieniipopulations and oneT. chinensisvar.maireipopulation. Of these loci, 10 were polymorphic inP. chienii, whereas six were polymorphic inT. chinensisvar.mairei. The unbiased haploid diversity per locus ranged from 0.000 to 0.641 and 0.000 to 0.545 forP. chieniiandT. chinensisvar.mairei, respectively.


CONCLUSIONS
The 18 cpSSRs will be used to further investigate the chloroplast genetic structure and adaptive evolution inP. chieniipopulations.
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China; School of Medicine, Guangxi University of Science and Technology, Liuzhou 545005, People's Republic of China.
Qi
Q
Deng
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Hanrui
H
Zhang
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Yipeng
Y
He
College of Life Science, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Ting
T
Wang
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Yingjuan
Y
Su
Pseudotaxus chienii; Taxaceae; Taxus chinensis var. mairei; chloroplast microsatellite; genetic diversity