Enhanced podocyte vesicle transport in the nephrotic rat.
Albumin endocytosis is enhanced in the podocytes of minimal change nephrotic syndrome. We investigated that the endocytic vesicle transport in the podocyte using three-dimensional observation in a rat model of puromycin aminonucleoside (PAN)-induced nephrotic syndrome. At day 7, Evans Blue-labeled albumin was intravenously injected in PAN rats, and one kidney was fixed for a morphological analysis; the other was used for the isolation of glomeruli through sieving and protein analyses. Evans Blue-labeled albumin was found to accumulate in an increased number of vesicles in the podocytes of PAN rat. Continuous sections and its three-dimensional observation demonstrated that vesicles may be transported from the cytoplasm to the apical membrane of the podocytes. The increased protein bands in the gel electrophoresis of the sieved glomeruli of nephrotic rats were analyzed by mass spectrometry in comparison to the control rats. The major proteins increased in the nephrotic rats were cytoplasmic dynein 1 heavy chain, myosin IX, and myosin VIIb. In conclusion, the podocyte endocytic vesicles carrying albumin increased with glomerular cytoplasmic dynein and myosin in minimal change nephrotic rats.
Division of Nephrology and Endocrinology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan. akitojo-tky@umin.ac.jp.
Akihiro
http://orcid.org/0000-0002-9315-7546
A
Tojo
Division of Nephrology and Endocrinology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
Saaya
S
Hatakeyama
Division of Nephrology and Endocrinology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
Satoshi
S
Kinugasa
Laboratory of Electron Microscopy, The University of Tokyo, Tokyo, Japan.
Satoru
S
Fukuda
Sakai Electron Microscope Application Laboratory, Saitama, Japan.
Toshio
T
Sakai
D000418:Albumins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D056445:Cytoplasmic Dyneins / Q000378:metabolism; D004705:Endocytosis; D005070:Evans Blue / Q000737:chemistry; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D009218:Myosins / Q000378:metabolism; D009404:Nephrotic Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D050199:Podocytes / Q000378:metabolism* / Q000473:pathology; D020033:Protein Isoforms / Q000378:metabolism; D011692:Puromycin Aminonucleoside; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods; D022161:Transport Vesicles / Q000737:chemistry / Q000378:metabolism*
D000418:Albumins; D020033:Protein Isoforms; C113469:myosin IXB; D005070:Evans Blue; D011692:Puromycin Aminonucleoside; D009218:Myosins; D056445:Cytoplasmic Dyneins
Albumin; Dynein; Endocytosis; Myosin; Nephrotic syndrome; Podocyte; Proteinuria