SIDER2 retroposon-mediated mRNA decay in Leishmania is coupled to translation.
We previously reported that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily predominantly located within 3' untranslated regions (UTRs) of Leishmania transcripts promote rapid turnover that is initiated by endonucleolytic cleavage. Here, we investigated whether SIDER2-mediated mRNA decay is linked to translation. We show that preventing translation initiation by inserting a hairpin structure at the 5'-end of a SIDER2-containing mRNA blocks degradation. Similarly, global inhibition of translation elongation by cycloheximide or termination by puromycin causes stabilisation of SIDER2-containing transcripts. Altogether, these findings support that the mechanism of SIDER2-mediated decay is coupled to translation, possibly through the recruitment of decay factors to elongating ribosomes.
Research Center in Infectious Disease, CHU de Quebec Research Center, 2705 Laurier Blvd., Quebec G1V 4G2, QC, Canada; Department of Microbiology-Infectious Disease and Immunology, Faculty of Medicine, University Laval, Quebec, Canada.
Hiva
H
Azizi
Institute for Cell Biology and Neuroscience, Goethe University Frankfurt, Max-von-Laue-Str. 13, 60438 Frankfurt am Main, Frankfurt, Germany.
Michaela
M
Müller-McNicoll
Research Center in Infectious Disease, CHU de Quebec Research Center, 2705 Laurier Blvd., Quebec G1V 4G2, QC, Canada; Department of Microbiology-Infectious Disease and Immunology, Faculty of Medicine, University Laval, Quebec, Canada. Electronic address: barbara.papadopoulou@crchul.ulaval.ca.
Barbara
B
Papadopoulou
D020413:3' Untranslated Regions / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D007891:Leishmania / Q000187:drug effects / Q000235:genetics*; D018314:Leishmania infantum / Q000187:drug effects; D014176:Protein Biosynthesis / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects; D020086:Short Interspersed Nucleotide Elements / Q000502:physiology*; D014178:Translocation, Genetic / Q000187:drug effects
D020413:3' Untranslated Regions; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D003513:Cycloheximide
Cycloheximide; Leishmania; MS2 hairpin; Puromycin; SIDER2 retroposon; Translation; mRNA decay