Knockdown of SKA1 gene inhibits cell proliferation and metastasis in human adenoid cystic carcinoma.
The spindle and kinetochore-associated complex subunit 1(SKA1) is a newly discovered gene, which has been associated with mitosis and tumorigenesis. However, its role insalivary adenoid cystic carcinoma (SACC) is still unknown, and the invasive and metastatic mechanism in SACC is still unclear. To explore the molecular mechanism of SKA1 in the process of malignant proliferation and metastasis in adenoid cystic cancer (ACC) cells, we employed lentivirus-mediated short hairpin RNA to knockdown SKA1 in SACC-83 cells. The results demonstrated that the lentivirus-mediated shRNA-targeting SKA1 lead to a significant down-regulation of SKA1 expression. Knockdown of SKA1 inhibited cell proliferation, cell invasion, migration and the cell cycle arrest. Furthermore, knockdown of SKA1 reduced the Ndc80, CDK4, Cyclin D1, Cyclin E1, Cyclin B1 and matrix metalloproteinase-9 (MMP-9) protein expression, but increased the p27 protein expression. These findings indicated that SKA1 might be a promising target for cancer gene therapy in human ACC.
School of Stomatology, Shandong University, Jinan, Shandong, 250100, China; Yantai Stomatological Hospital, Yantai, 264000, China.
Li-Juan
LJ
Zhao
Liaocheng People's Hospital and Clinical School of Taishan Medical University, Liaocheng, Shandong, 252000, China.
Hong-Li
HL
Yang
Liaocheng People's Hospital and Clinical School of Taishan Medical University, Liaocheng, Shandong, 252000, China.
Ke-Yi
KY
Li
School of Stomatology, Binzhou Medical College, Yantai, 264000, China.
Yue-Hua
YH
Gao
Yantai Stomatological Hospital, Yantai, 264000, China.
Kai
K
Dong
Yantai Stomatological Hospital, Yantai, 264000, China.
Zhong-Hao
ZH
Liu
School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia. Electronic address: lwang@csu.edu.au.
Le-Xin
LX
Wang
School of Stomatology, Shandong University, Jinan, Shandong, 250100, China; Liaocheng People's Hospital and Clinical School of Taishan Medical University, Liaocheng, Shandong, 252000, China. Electronic address: zhangbin252000@163.com.
Bin
B
Zhang
D003528:Carcinoma, Adenoid Cystic / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D055785:Gene Knockdown Techniques / Q000379:methods; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics
D002868:Chromosomal Proteins, Non-Histone; D034741:RNA, Small Interfering; C516427:SKA1 protein, human
Metastasis; Proliferation; RNA interference; Salivary adenoid cystic carcinoma (SACC); Spindle and kinetochore-associated complex subunit 1(SKA1)