Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors.
In this chapter, you will learn how to use translocation biosensors to investigate protease functions in living cells. We here present modular protein translocation biosensors tailored to investigate protease activity and protein-protein interactions. Besides the mapping of protease function, the biosensors are also applicable to identify chemicals and/or (nano)materials modulating the respective protein activities and can also be exploited for RNAi-mediated genetic screens.
Molecular and Cellular Oncology, ENT/University Medical Center Mainz, Langenbeckstr. 1, 55131, Mainz, Germany. angelinahahlbrock@uni-mainz.de.
Angelina
A
Hahlbrock
Molecular and Cellular Oncology, ENT/University Medical Center Mainz, Langenbeckstr. 1, 55131, Mainz, Germany.
Dorothée
D
Gößwein
Molecular and Cellular Oncology, ENT/University Medical Center Mainz, Langenbeckstr. 1, 55131, Mainz, Germany.
Roland H
RH
Stauber
D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods; D055808:Drug Discovery / Q000379:methods*; D010450:Endopeptidases / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D057166:High-Throughput Screening Assays; D008856:Microscopy, Fluorescence; D011480:Protease Inhibitors / Q000494:pharmacology*; D011485:Protein Binding; D021381:Protein Transport; D059748:Proteolysis; D013379:Substrate Specificity
D011480:Protease Inhibitors; D010450:Endopeptidases
Chemical biology; Fluorescence microscopy; High content screening; Protease; Protein-protein interaction; Translocation