Are cells from a snowman realistic? Cryopreserved tissues as a source for single-cell RNA-sequencing experiments.
A recently published study in Genome Biology shows that cells isolated from cryopreserved tissues are a reliable source of genetic material for single-cell RNA-sequencing experiments.Please see related Method article: http://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1171-9.
Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. fvb@sanger.ac.uk.
Felipe A
FA
Vieira Braga
Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.|European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SD, UK.|Cavendish Laboratory, Cambridge University, JJ Thomson Avenue, Cambridge, CB3 0HE, UK.
Sarah A
SA
Teichmann
Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. ms31@sanger.ac.uk.
Michael J T
MJ
Stubbington
D000818:Animals; D006801:Humans; D015925:Cryopreservation; D012313:RNA / Q000235:genetics; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis / Q000379:methods
D012313:RNA