Evolution of the Genome 3D Organization: Comparison of Fused and Segregated Globin Gene Clusters.
The genomes are folded in a complex three-dimensional (3D) structure. Some features of this organization are common for all eukaryotes, but little is known about its evolution. Here, we have studied the 3D organization and regulation of zebrafish globin gene domain and compared its organization and regulation with those of other vertebrate species. In birds and mammals, the α- and β-globin genes are segregated into separate clusters located on different chromosomes and organized into chromatin domains of different types, whereas in cold-blooded vertebrates, including Danio rerio, α- and β-globin genes are organized into common clusters. The major globin gene locus of Danio rerio is of particular interest as it is located in a genomic area that is syntenic in vertebrates and is controlled by a conserved enhancer. We have found that the major globin gene locus of Danio rerio is structurally and functionally segregated into two spatially distinct subloci harboring either adult or embryo-larval globin genes. These subloci demonstrate different organization at the level of chromatin domains and different modes of spatial organization, which appears to be due to selective interaction of the upstream enhancer with the sublocus harboring globin genes of the adult type. These data are discussed in terms of evolution of linear and 3D organization of gene clusters in vertebrates.
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.|Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Anastasia P
AP
Kovina
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.
Natalia V
NV
Petrova
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.
Ekaterina S
ES
Gushchanskaya
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.
Konstantin V
KV
Dolgushin
Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Evgeny S
ES
Gerasimov
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.|Faculty of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, Moscow, Russia.|Institute for Information Transmission Problems (The Kharkevich Institute) of the Russian Academy of Sciences, Moscow, Russia.
Aleksandra A
AA
Galitsyna
Institute for Information Transmission Problems (The Kharkevich Institute) of the Russian Academy of Sciences, Moscow, Russia.|Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
Alexey A
AA
Penin
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.
Ilya M
IM
Flyamer
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.|LIA 1066 French-Russian Joint Cancer Research Laboratory, Villejuif, France.
Elena S
ES
Ioudinkova
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.
Alexey A
AA
Gavrilov
LIA 1066 French-Russian Joint Cancer Research Laboratory, Villejuif, France.|CNRSUMR8126, Institut Gustave Roussy, Université Paris Sud Paris Saclay, Villejuif, France.
Yegor S
YS
Vassetzky
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.|Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Sergey V
SV
Ulianov
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.|LIA 1066 French-Russian Joint Cancer Research Laboratory, Villejuif, France.
Olga V
OV
Iarovaia
Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.|Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.|LIA 1066 French-Russian Joint Cancer Research Laboratory, Villejuif, France.
Sergey V
SV
Razin
D000818:Animals; D005075:Biological Evolution; D001717:Birds / Q000235:genetics; D002843:Chromatin / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics; D019143:Evolution, Molecular; D016678:Genome; D005914:Globins / Q000235:genetics*; D008322:Mammals / Q000235:genetics; D008968:Molecular Conformation; D005810:Multigene Family / Q000235:genetics; D015027:Zebrafish / Q000235:genetics; D055542:alpha-Globins / Q000235:genetics; D055544:beta-Globins / Q000235:genetics
D002843:Chromatin; D055542:alpha-Globins; D055544:beta-Globins; D005914:Globins
3D organization of the genome; Danio rerio; chromatin; globin genes