Proteomic analysis of physiological function response to hot summer in liver from lactating dairy cows.
Lactation performance of dairy cattle is susceptible to heat stress. The liver is one of the most crucial organs affected by high temperature in dairy cows. However, the physiological adaption by the liver to hot summer conditions has not been well elucidated in lactating dairy cows. In the present study, proteomic analysis of the liver in dairy cows in spring and hot summer was performed using a label-free method. In total, 127 differentially expressed proteins were identified; most of the upregulated proteins were involved in protein metabolic processes and responses to stimuli, whereas most of the downregulated proteins were related to oxidation-reduction. Pathway analysis indicated that 3 upregulated heat stress proteins (HSP90α, HSP90β, and endoplasmin) were enriched in the NOD-like receptor signaling pathway, whereas several downregulated NADH dehydrogenase proteins were involved in the oxidative phosphorylation pathway. The protein-protein interaction network indicated that several upregulated HSPs (HSP90α, HSP90β, and GRP78) were involved in more interactions than other proteins and were thus considered as central hub nodes. Our findings provide novel insights into the physiological adaption of liver function in lactating dairy cows to natural high temperature.
Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Qiangjun
Q
Wang
Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Xiaowei
X
Zhao
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Zijun
Z
Zhang
Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Huiling
H
Zhao
Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Dongwei
D
Huang
Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Guanglong
G
Cheng
Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China. Electronic address: yyongxin@yahoo.com.
Yongxin
Y
Yang
D000222:Adaptation, Physiological; D000818:Animals; D002417:Cattle / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D007774:Lactation; D008099:Liver / Q000502:physiology*; D060066:Protein Interaction Maps; D040901:Proteomics; D012621:Seasons; D015398:Signal Transduction; D013312:Stress, Physiological
D006360:Heat-Shock Proteins
Animal proteomics; Cow; Hot summer; LC-MS/MS; Liver tissue