Increased interleukin-1β and basic fibroblast growth factor levels in the cerebrospinal fluid during human herpesvirus-6B (HHV-6B) encephalitis.
Human herpesvirus 6B (HHV-6B) causes exanthema subitum in infants and is known to be mildly pathogenic. However, HHV-6B infection can induce febrile seizures in a high percentage of patients, and in rare cases, result in encephalitis. We detected higher levels of interleukin (IL)-1β and basic fibroblast growth factor (bFGF) in the cerebrospinal fluid (CFS) of patients with HHV-6B encephalitis when compared to those in patients with non-HHV-6B-induced febrile seizures. In vitro, IL-1β and bFGF enhanced HHV-6B gene expression in infected U373 astrocytes during the initial and maintenance phases of infection, respectively. These findings indicated that IL-1β and bFGF contribute to HHV-6B growth and the onset of encephalitis.
Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan; Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Masato
M
Tamai
Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan. Electronic address: kobayashi-n@jikei.ac.jp.
Nobuyuki
N
Kobayashi
Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Kazuya
K
Shimada
Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Naomi
N
Oka
Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Mayumi
M
Takahashi
Department of Obstetrics and Gynecology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Akiko
A
Tanuma
Department of Obstetrics and Gynecology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan; Department of General Medical Science, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba-shi, Chiba 260-8670, Japan.
Tomohiro
T
Tanemoto
Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Hiroyuki
H
Namba
Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Yoshihiro
Y
Saito
Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Yasuyuki
Y
Wada
Department of Obstetrics and Gynecology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Aikou
A
Okamoto
Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Hiroyuki
H
Ida
Department of Virology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan.
Kazuhiro
K
Kondo
D001253:Astrocytes / Q000378:metabolism / Q000821:virology; D016022:Case-Control Studies; D002460:Cell Line; D002675:Child, Preschool; D004279:DNA, Viral / Q000134:cerebrospinal fluid / Q000235:genetics*; D018792:Encephalitis, Viral / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000473:pathology / Q000821:virology; D005260:Female; D005346:Fibroblast Growth Factors / Q000134:cerebrospinal fluid / Q000235:genetics*; D015870:Gene Expression; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D006801:Humans; D007223:Infant; D053583:Interleukin-1beta / Q000134:cerebrospinal fluid / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000134:cerebrospinal fluid / Q000235:genetics; D003294:Seizures, Febrile / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000473:pathology / Q000821:virology
D004279:DNA, Viral; C508585:IL1B protein, human; D053583:Interleukin-1beta; D012333:RNA, Messenger; C108111:fibroblast growth factor 13; D005346:Fibroblast Growth Factors
Astrocytes; Basic fibroblast growth factor; Encephalitis; Human herpesvirus-6; Interleukin-1β