Aroma compound diacetyl suppresses glucagon-like peptide-1 production and secretion in STC-1 cells.
Diacetyl is a volatile flavour compound that has a characteristic buttery aroma and is widely used in the flavour industry. The aroma of a food plays an important role in food palatability and thus intake. This study investigates the effect of diacetyl on the satiety hormone, glucagon-like peptide (GLP-1), using the enteroendocrine cell line, STC-1. Diacetyl decreased proglucagon mRNA and total GLP-1 from glucose stimulated STC-1 cells. This dampening effect on GLP-1 appears to be mediated by increasing intracellular cAMP levels, increasing synthesis of the G protein coupled receptor, GPR120, and its recruitment to the cell surface. Voltage gated Ca2+channels, K+ATPchannels and the α-gustducin taste pathway do not appear to be involved. These findings demonstrate that components contributing to food palatability suppress GLP-1. This ability of diacetyl to reduce satiety signals may contribute to overconsumption of some palatable foods.
Food for Health Ireland, Teagasc Food Research Centre Moorepark, Fermoy, Cork, Ireland; Food for Health Ireland, Dept of Anatomy and Neuroscience, University College Cork, Ireland. Electronic address: triona.mccarthy@hotmail.com.
Triona
T
McCarthy
Food for Health Ireland, Teagasc Food Research Centre Moorepark, Fermoy, Cork, Ireland. Electronic address: bruen_christine@lilly.com.
Christine
C
Bruen
Food for Health Ireland, Teagasc Food Research Centre Moorepark, Fermoy, Cork, Ireland. Electronic address: Fiona.OHalloran@cit.ie.
Fiona
F
O'Halloran
Food for Health Ireland, Dept of Anatomy and Neuroscience, University College Cork, Ireland. Electronic address: H.Schellekens@ucc.ie.
Harriet
H
Schellekens
Teagasc Food Research Centre Moorepark, Fermoy, Cork, Ireland. Electronic address: Kieran.Kilcawley@teagasc.ie.
Kieran
K
Kilcawley
Food for Health Ireland, Dept of Anatomy and Neuroscience, University College Cork, Ireland. Electronic address: J.Cryan@ucc.ie.
John F
JF
Cryan
Food for Health Ireland, Teagasc Food Research Centre Moorepark, Fermoy, Cork, Ireland. Electronic address: linda.giblin@teagasc.ie.
Linda
L
Giblin
D000818:Animals; D003931:Diacetyl / Q000737:chemistry*; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D012528:Satiety Response; D012903:Smell
D052216:Glucagon-Like Peptide 1; D003931:Diacetyl
Diacetyl; Diacetyl (PubChem CID:650); Flavour compound; GPR120; Glucagon-like peptide 1; Nicardipine (PubChem CID:41114); Nitrendipine (PubChem CID:4507); Satiety; Tolbutamide (PubChem CID:5505)