Introduction to Modern Methods in Light Microscopy.
For centuries, light microscopy has been a key method in biological research, from the early work of Robert Hooke describing biological organisms as cells, to the latest in live-cell and single-molecule systems. Here, we introduce some of the key concepts related to the development and implementation of modern microscopy techniques. We briefly discuss the basics of optics in the microscope, super-resolution imaging, quantitative image analysis, live-cell imaging, and provide an outlook on active research areas pertaining to light microscopy.
LMU Munich, Biocenter Martinsried, Grosshadernerstr. 2, 82152, Martinsried, Munich, Germany.
Joel
J
Ryan
Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, QC, Canada.
Abby R
AR
Gerhold
University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Vincent
V
Boudreau
University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Lydia
L
Smith
Department of Biology, University of North Carolina at Chapel Hill, 4358 Genome Sciences Building, Chapel Hill, NC, 27599, USA. pmaddox@unc.edu.
Paul S
PS
Maddox
D000818:Animals; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008853:Microscopy / Q000145:classification / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D055095:Optics and Photonics
Image analysis; Live-cell; Microscopy; Super-resolution; Technology