Transplantation of mesenchymal stem cells overexpressing IL10 attenuates cardiac impairments in rats with myocardial infarction.
Mesenchymal stem cell (MSC) has been well known to exert therapeutic potential for patients with myocardial infarction (MI). In addition, interleukin-10 (IL10) could attenuate MI through suppressing inflammation. Thus, the combination of MSC implantation with IL10 delivery may extend health benefits to ameliorate cardiac injury after MI. Here we established overexpression of IL10 in bone marrow-derived MSC through adenoviral transduction. Cell viability, apoptosis, and IL10 secretion under ischemic challenge in vitro were examined. In addition, MSC was transplanted into the injured hearts in a rat model of MI. Four weeks after the MI induction, MI, cardiac functions, apoptotic cells, and inflammation cytokines were assessed. In response to in vitro oxygen-glucose deprivation (OGD), IL10 overexpression in MSC (Ad.IL10-MSC) enhanced cell viability, decreased apoptosis, and increased IL10 secretion. Consistently, the implantation of Ad.IL10-MSCs into MI animals resulted in more reductions in myocardial infarct size, cardiac impairment, and cell apoptosis, compared to the individual treatments of either MSC or IL10 administration. Moreover, the attenuation of both systemic and local inflammations was most prominent for Ad.IL10-MSC treatment. IL10 overexpression and MSC may exert a synergistic anti-inflammatory effect to alleviate cardiac injury after MI.
Department of Ultrasonography, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Xin
X
Meng
Department of Radiation Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Jianping
J
Li
Department of Ultrasonography, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Ming
M
Yu
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Jian
J
Yang
Department of Ultrasonography, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Minjuan
M
Zheng
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Jinzhou
J
Zhang
Department of Ultrasonography, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Chao
C
Sun
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Hongliang
H
Liang
Department of Ultrasonography, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Liwen
http://orcid.org/0000-0002-6632-8290
L
Liu
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia; D002470:Cell Survival; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D005947:Glucose / Q000172:deficiency; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014161:Transduction, Genetic; D014162:Transfection; D015854:Up-Regulation; D016277:Ventricular Function, Left; D017725:Ventricular Pressure
D016753:Interleukin-10; D005947:Glucose
cardiac injury; inflammation; interleukin-10; mesenchymal stem cell; myocardial infarction