Physiological and biochemical responses of Salix integra Thunb. under copper stress as affected by soil flooding.
To explore the joint effect of copper (Cu) and flooding on Salix integra Thunb. (S. integra), the physiological and biochemical parameters of the seedlings grown in Cu amended soil (50, 150, 450 mg kg-1) with or without the flooding for 60 days were evaluated. The results suggested that the flooding significantly inhibited the root growth in terms of root length and root tips. The Cu exposures of 50 and 150 mg kg-1notably enhanced the root growth as compared to the control. Majority of Cu was accumulated in S. integra roots, while flooding significantly reduced the Cu content, except the 150 mg kg-1Cu treatment, but the iron (Fe) and manganese (Mn) content on the root surface were both markedly increased relative to non-flooded control. The malonaldehyde (MDA) and glutathione (GSH) contents in leaves showed a dose-response upon Cu exposure. Soil flooding enhanced the GSH level, which displayed 4.50-49.59% increases compared to its respective non-flooded treatment, while no difference was evident on MDA contents between the flooding and the non-flooded treatments. Both superoxide dismutase (SOD) and peroxidase (POD) activities were boosted while the catalase (CAT) was suppressed with increasing Cu exposure dose, and soil flooding reduced the POD and CAT activities. The elevated Cu level caused the evident increases of root calcium (Ca), potassium (K), and sulfur (S) concentrations and decreases of root phosphorus (P), sodium (Na), and zinc (Zn) concentrations. Soil flooding increased the concentrations of Fe, S, Na, Ca, and magnesium (Mg) in S. integra root. Taken together, our results suggested S. integra has high tolerance to the joint stress from Cu and flooding.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.
Yini
Y
Cao
Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, United States; Department of Analytical Chemistry, The Connecticut Agricultural Experiment Station, New Haven, CT 06504, United States.
Chuanxin
C
Ma
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China. Electronic address: guangcaichen@sohu.com.
Guangcai
G
Chen
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, China.
Jianfeng
J
Zhang
Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, United States.
Baoshan
B
Xing
D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000032:analysis / Q000633:toxicity*; D055868:Floods; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide; D007501:Iron / Q000032:analysis; D008315:Malondialdehyde; D008345:Manganese / Q000032:analysis; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000378:metabolism; D032108:Salix / Q000378:metabolism / Q000502:physiology*; D036226:Seedlings / Q000187:drug effects; D012987:Soil; D013482:Superoxide Dismutase / Q000378:metabolism; D018675:Toxicity Tests; D015032:Zinc / Q000032:analysis
D012987:Soil; D008345:Manganese; D008315:Malondialdehyde; D003300:Copper; D006861:Hydrogen Peroxide; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D015032:Zinc
Copper; Flooding; Nutrients; Oxidative stress; Phytoremediation; Willow