Tridimensional Visualization and Analysis of Early Human Development.
Generating a precise cellular and molecular cartography of the human embryo is essential to our understanding of the mechanisms of organogenesis in normal and pathological conditions. Here, we have combined whole-mount immunostaining, 3DISCO clearing, and light-sheet imaging to start building a 3D cellular map of the human development during the first trimester of gestation. We provide high-resolution 3D images of the developing peripheral nervous, muscular, vascular, cardiopulmonary, and urogenital systems. We found that the adult-like pattern of skin innervation is established before the end of the first trimester, showing important intra- and inter-individual variations in nerve branches. We also present evidence for a differential vascularization of the male and female genital tracts concomitant with sex determination. This work paves the way for a cellular and molecular reference atlas of human cells, which will be of paramount importance to understanding human development in health and disease. PAPERCLIP.
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France.
Morgane
M
Belle
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France.
David
D
Godefroy
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France.
Gérard
G
Couly
UMR-S 1172, JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, University of Lille, Lille 59000, France; INSERM, UMR-S 1172, Laboratory of Development and Plasticity of the Neuroendocrine Brain, Lille 59000, France.
Samuel A
SA
Malone
FHU 1,000 Days for Health, School of Medicine, University of Lille, Lille 5900, France; Gynaecology Service, Hospital Jeanne de Flandres, CHU Lille, Lille 59000, France.
Francis
F
Collier
UMR-S 1172, JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, University of Lille, Lille 59000, France; INSERM, UMR-S 1172, Laboratory of Development and Plasticity of the Neuroendocrine Brain, Lille 59000, France; FHU 1,000 Days for Health, School of Medicine, University of Lille, Lille 5900, France.
Paolo
P
Giacobini
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France. Electronic address: alain.chedotal@inserm.fr.
Alain
A
Chédotal
D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005333:Fetus / Q000166:cytology*; D006803:Human Development; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D038081:Organogenesis; D017933:Peripheral Nervous System / Q000166:cytology / Q000254:growth & development
3DISCO; database; embryo; fetus; human; iDISCO; light sheet microscopy; nervous system; tissue clearing; urogenital system