ProbioticLactobacillus reuterihas antifungal effects on oralCandidaspeciesin vitro.
Background:An alternative approach for managingCandidainfections in the oral cavity by modulating the oral microbiota with probiotic bacteria has been proposed.Objective:The aim was to investigate the antifungal potential of the probiotic bacteriumLactobacillus reuteri(DSM 17938 and ATCC PTA 5289) against six oralCandidaspecies (C. albicans, C. glabrata, C. krusei, C. tropicalis, C. dubliniensis, andC. parapsilosis).Design:The lactobacilli were tested for their ability to co-aggregate with and inhibit the growth of the yeasts assessed by spectrophotometry and the agar overlay inhibition assay. Additionally, the pH was evaluated with microsensors, and the production of hydrogen peroxide (H2O2) by the lactobacilli was verified.Results:BothL. reuteristrains showed co-aggregation abilities with the yeasts. The lactobacilli almost completely inhibited the growth ofC. albicansandC. parapsilosis, but did not affectC. krusei. Statistically significant differences in co-aggregation and growth inhibition capacities between the twoL. reuteristrains were observed (p<0.001). The pH measurements suggested thatC. kruseican resist the acids produced by the lactobacilli.Conclusions:L. reuteriexhibited antifungal properties against five of the six most common oral Candida species. Further, the results reconfirms that the probiotic capacity ofL. reuteriis strain specific.
Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark.
Mette Rose
MR
Jørgensen
Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark.
Camilla
C
Kragelund
Department of Clinical Microbiology, Rigshospitalet , Copenhagen , Denmark.
Peter Østrup
PØ
Jensen
Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark.
Mette Kirstine
MK
Keller
Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark.
Svante
S
Twetman
Beneficial bacteria; antimycotic; candidiasis; co-aggregation; growth inhibition; yeast