Nanoparticles Composed of Zn and ZnO InhibitPeronospora tabacinaSpore Germinationin vitroandP. tabacinaInfectivity on Tobacco Leaves.
Manufactured nanoparticles (NPs) are increasingly being used for commercial purposes and certain NP types have been shown to have broad spectrum antibacterial activity. In contrast, their activities against fungi and fungi-like oomycetes are less studied. Here, we examined the potential of two types of commercially available Zn NPs (Zn NPs and ZnO NPs) to inhibit spore germination and infectivity on tobacco leaves resulting from exposure to the fungi-like oomycete pathogenPeronospora tabacina(P. tabacina). Both types of NPs, as well as ZnCl₂ and bulk ZnO control treatments, inhibited spore germination compared to a blank control. ZnO ENMs were shown to be a much more powerful suppressor of spore germination and infectivity than bulk ZnO. ZnO and Zn NPs significantly inhibited leaf infection at 8 and 10 mg·L-1, respectively. Both types of NPs were found to provide substantially higher concentration dependent inhibition of spore germination and infectivity than could be readily explained by the presence of dissolved Zn. These results suggest that both NP types have potential for use as economic, low-dose, potentially non-persistent anti-microbial agents against the oomyceteP. tabacina.
Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA. george.wagner@uky.edu.
George
G
Wagner
Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA. vdkore0@uky.edu.
Victor
V
Korenkov
Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA. jonathan.judy@csiro.au.|CSIRO Land and Water, Urrbrae 5064, Australia. jonathan.judy@csiro.au.
Jonathan D
JD
Judy
Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA. paul.bertsch@csiro.au.|CSIRO Land and Water Flagship, CSIRO, Dutton Park 4102, Australia. paul.bertsch@csiro.au.
Paul M
PM
Bertsch
nanomaterials; nanopesticides; nanotechnology; nanotoxicity; nanotoxicology