Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay.
Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies.
Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia.
Eliana
E
Baez-Jurado
Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia.
Gina Guio
GG
Vega
Institute of Physiologically Active Compounds Russian Academy of Sciences, Chernogolovka, 142432, Russia.|GALLY International Biomedical Research Consulting LLC, San Antonio, TX, 78229, USA.|School of Health Science and Healthcare Administration, University of Atlanta, Johns Creek, GA, 30097, USA.
Gjumrakch
G
Aliev
Institute of Pharmacy and Translational Medicine, Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya st., 119991, Moscow, Russia.
Vadim V
VV
Tarasov
Facultad Medicina Veterinaria y Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia.
Paula
P
Esquinas
Facultad Ciencias de la Salud, Universidad San Sebastián, Lientur 1457, 4030000, Concepción, Chile.
Valentina
V
Echeverria
Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia. gsampaio@javeriana.edu.co.|Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Santiago, Chile. gsampaio@javeriana.edu.co.
George E
GE
Barreto
D000328:Adult; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D000078787:Neuroglobin / Q000037:antagonists & inhibitors* / Q000502:physiology*; D018696:Neuroprotective Agents / Q000008:administration & dosage; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010812:Physical Stimulation / Q000009:adverse effects; D055815:Young Adult
D017077:Culture Media, Conditioned; C000627156:NGB protein, human; D000078787:Neuroglobin; D018696:Neuroprotective Agents
Adipose tissue; Astrocytes; Conditioned medium; Mesenchymal stem cells; Neuroglobin; Scratch assay