How to induce defense responses in wild plant populations? Using bilberry (Vaccinium myrtillus) as example.
Inducible plant defense is a beneficial strategy for plants, which imply that plants should allocate resources from growth and reproduction to defense when herbivores attack. Plant ecologist has often studied defense responses in wild populations by biomass clipping experiments, whereas laboratory and greenhouse experiments in addition apply chemical elicitors to induce defense responses. To investigate whether field ecologists could benefit from methods used in laboratory and greenhouse studies, we established a randomized block-design in a pine-bilberry forest in Western Norway. We tested whether we could activate defense responses in bilberry (Vaccinium myrtillus) by nine different treatments using clipping (leaf tissue or branch removal) with or without chemical treatment by methyljasmonate (MeJA). We subsequently measured consequences of induced defenses through vegetative growth and insect herbivory during one growing season. Our results showed that only MeJA-treated plants showed consistent defense responses through suppressed vegetative growth and reduced herbivory by leaf-chewing insects, suggesting an allocation of resources from growth to defense. Leaf tissue removal reduced insect herbivory equal to the effect of the MeJa treatments, but had no negative impact on growth. Branch removal did not reduce insect herbivory or vegetative growth. MeJa treatment and clipping combined did not give an additional defense response. In this study, we investigated how to induce defense responses in wild plant populations under natural field conditions. Our results show that using the chemical elicitor MeJA, with or without biomass clipping, may be a better method to induce defense response in field experiments than clipping of leaves or branches that often has been used in ecological field studies.
Faculty of Engineering and Science Sogn & Fjordane University College Sogndal Norway.
Tarald
T
Seldal
Faculty of Engineering and Science Sogn & Fjordane University College Sogndal Norway.
Stein Joar
SJ
Hegland
Faculty of Engineering and Science Sogn & Fjordane University College Sogndal Norway.
Knut
K
Rydgren
Department of Entomology Rutgers University Chatsworth NJ USA.
Cesar
C
Rodriguez-Saona
Faculty of Engineering and Science Sogn & Fjordane University College Sogndal Norway; Norwegian Institute of Nature Research Bergen Norway.
Joachim Paul
JP
Töpper
Vaccinium; bilberry; chemical elicitor; clipping experiment; deer; field experiment; growth; herbivory; insects; methyljasmonate