Modulation of vascular function and anti-aggregation effect induced by (1→3) (1→6)-β-d-glucan of Saccharomyces cerevisiae and its carboxymethylated derivative in rats.
BACKGROUND
β-d-Glucans are polysaccharides found in the cell walls of yeasts, such as Saccharomyces cerevisiae, and they have been studied because of their beneficial effects on health, mainly in terms of immunomodulation. However, information on the action of these polymers on vascular and platelet function is still scarce. This study evaluate the effect of (1→3) (1→6) β-d-glucan (βG-Sc) and its carboxymethylated derivative (CM-G) on vascular and platelet function in rats.


METHODS
The animals received daily oral treatments with βG-Sc (20mg/kg) and CM-G (20mg/kg) for eight days. Next, cytokine quantification, vascular reactivity and adenosine diphosphate (ADP)- and collagen-induced platelet aggregation studies were performed. In vitro platelet aggregation and P-selectin exposition assays were conducted using 100 and 300μg/mL CM-G.


RESULTS
The CM-G-treated group had less IL-8 than did the control. In reactivity experiments, CM-G and βG-Sc treatments did not change the contractile response of the vessel induced by PHE. Moreover, only CM-G improved the vasorelaxation response to Nitroprusside (SPN, a nitric oxide donor). The in vitro aggregation studies showed that at the highest concentration (300μg/mL), CM-G inhibited the agonist-induced platelet aggregation with an effect similar to that of acetylsalicylic acid and without affecting P-selectin exposition. The treatments with βG-Sc or CM-G inhibited the platelet aggregation stimulated by ADP, but only βG-Sc treatment was effective in affect the collagen-stimulated aggregation.


CONCLUSIONS
These findings suggest that CM-G modulate positively the vascular function, mainly in responses NO-dependent. CM-G and βG-Sc have an anti-aggregation effect, being CM-G more selective to ADP-induced platelet aggregation.
Graduate Program in Nutritional Sciences, Health Sciences Center, Federal University of Paraíba (UFPB), João Pessoa, Paraíba, Brazil.
Lorena Soares
LS
Bezerra
Graduate Program in Nutritional Sciences, Health Sciences Center, Federal University of Paraíba (UFPB), João Pessoa, Paraíba, Brazil; Department of Chemical and Food Technology, Technology Center, UFPB, João Pessoa, Paraíba, Brazil.
Marciane
M
Magnani
Department of Sciences and Technology, State University of Londrina, Londrina, Paraná, Brazil.
Raul Jorge Hernan
RJH
Castro-Gomez
Department of Nutrition, Health Sciences Center, UFPB, João Pessoa, Paraíba, Brazil.
Hassler Clementino
HC
Cavalcante
Department of Pharmaceutical Sciences, Health Sciences Center, UFPB, João Pessoa, Paraíba, Brazil.
Tays Amanda Felisberto da
TAFD
Silva
Graduate Program in Nutritional Sciences, Health Sciences Center, Federal University of Paraíba (UFPB), João Pessoa, Paraíba, Brazil.
Renata Layne Paixão
RLP
Vieira
Department of Pharmaceutical Sciences, Health Sciences Center, UFPB, João Pessoa, Paraíba, Brazil; Graduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, UFPB, João Pessoa, Paraíba, Brazil.
Isac Almeida de
IA
Medeiros
Graduate Program in Nutritional Sciences, Health Sciences Center, Federal University of Paraíba (UFPB), João Pessoa, Paraíba, Brazil; Department of Pharmaceutical Sciences, Health Sciences Center, UFPB, João Pessoa, Paraíba, Brazil. Electronic address: drrobveras@gmail.com.
Robson Cavalcante
RC
Veras
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D019007:P-Selectin / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D047071:beta-Glucans / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*
D020030:Nitric Oxide Donors; D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D047071:beta-Glucans; D009599:Nitroprusside; D009569:Nitric Oxide; D000244:Adenosine Diphosphate; D003094:Collagen; D001241:Aspirin
Carboxymethylated derivative; Platelet aggregation; Saccharomyces cerevisiae; Vascular reactivity; β-d-glucan