Transient Receptor Potential Melastatin 7 as an Independent Prognostic Factor in Human Esophageal Squamous Cell Carcinoma.
Transient receptor potential melastatin 7 (TRPM7) is a Mg2+/Ca2+-permeable channel with kinase activity and is expressed in many solid tumor types. However, it has not been evaluated in esophageal squamous cell carcinoma (ESCC). The aim of this study was to evaluate the prognostic impact of TRPM7 in ESCC and to determine the role of TRPM7 in proliferation, migration and invasion of ESCC.



Immunohistochemistry of TRPM7 for 52 primary tumor samples obtained from patients with ESCC undergoing esophagectomy was performed. Expression of TRPM7 in human ESCC cell lines was analyzed by real-time polymerase chain reaction (PCR). Knockdown experiments were conducted with TRPM7 siRNA, and the effect on cell proliferation, migration and invasion was analyzed.



Immunohistochemical staining showed that TRPM7 was found in the cytoplasm of carcinoma cells, and that expression was an independent prognostic factor of good postoperative survival. Suppression of TRPM7 expression with TRPM7-siRNA increased the proliferation, migration and invasion of TE5 ESCC cells.



The expression of TRPM7 has an impact on the prognosis of patients with ESCC.
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Shingo
S
Nakashima
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan shiozaki@koto.kpu-m.ac.jp.
Atsushi
A
Shiozaki
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Daisuke
D
Ichikawa
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Shoichiro
S
Hikami
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Toshiyuki
T
Kosuga
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Hirotaka
H
Konishi
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Shuhei
S
Komatsu
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Hitoshi
H
Fujiwara
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Kazuma
K
Okamoto
Department of Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Mitsuo
M
Kishimoto
Department of Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Eiichi
E
Konishi
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Eigo
E
Otsuji
D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D050053:TRPM Cation Channels / Q000235:genetics* / Q000378:metabolism*; D016896:Treatment Outcome
D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D050053:TRPM Cation Channels; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human
TRPM7; esophageal cancer; melastatin 7; prognostic factor