A validated fast difference spectrophotometric method for 5-hydroxymethyl-2-furfural (HMF) determination in corn syrups.
Corn syrups, important ingredients used in food and beverage industries, often contain high levels of 5-hydroxymethyl-2-furfural (HMF), a toxic contaminant. In this work, an in house validation of a difference spectrophotometric method for HMF analysis in corn syrups was developed using sophisticated statistical tools by the first time. The methodology showed excellent analytical performance with good selectivity, linearity (R2=99.9%, r>0.99), accuracy and low limits (LOD=0.10mgL-1and LOQ=0.34mgL-1). An excellent precision was confirmed by repeatability (RSD (%)=0.30) and intermediate precision (RSD (%)=0.36) estimates and by Horrat value (0.07). A detailed study of method precision using a nested design demonstrated that variation sources such as instruments, operators and time did not interfere in the variability of results within laboratory and consequently in its intermediate precision. The developed method is environmentally friendly, fast, cheap and easy to implement resulting in an attractive alternative for corn syrups quality control in industries and official laboratories.
Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil. Electronic address: jucimarakulekdeandrade@gmail.com.
Jucimara Kulek
JK
de Andrade
Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil.
Camila Kulek
CK
de Andrade
Chemistry Department, University of Manitoba, 144 Dysart Road, R3T 2N2 Winnipeg, MB, Canada.
Emy
E
Komatsu
Chemistry Department, University of Manitoba, 144 Dysart Road, R3T 2N2 Winnipeg, MB, Canada.
Hélène
H
Perreault
Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil.
Yohandra Reyes
YR
Torres
Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil.
Marcos Roberto
MR
da Rosa
Departamento de Química, Universidade Estadual do Centro-Oeste - UNICENTRO, 85040-080 Guarapuava, PR, Brazil.
Maria Lurdes
ML
Felsner
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003314:Corn Oil / Q000032:analysis*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry; D066248:High Fructose Corn Syrup / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*
D066248:High Fructose Corn Syrup; C008046:5-hydroxymethylfurfural; D003314:Corn Oil; D005662:Furaldehyde
Corn syrups; Difference spectrophotometry; HMF; In house validation; Nested design