Effects of microRNA-146a on the proliferation and apoptosis of human osteoarthritis chondrocytes by targetingTRAF6through the NF-κB signalling pathway.
The present study aims to investigate the effects ofmiR-146aon the proliferation and apoptosis of human osteoarthritis (OA) chondrocytes by targeting tumour necrosis factor receptor-associated factor 6 (TRAF6) through nuclear factor-κB (NF-κB) signalling pathway. Human normal and OA chondrocytes were selected and divided into the normal group, blank group, negative control (NC) group,miR-146amimics group,miR-146ainhibitors,miR-146ainhibitor + si-TRAF6 group and si-TRAF6 group. Quantitative real-time PCR (qRT-PCR) was applied to detect the expressions ofmiR-146a,TRAF6mRNA andNF-κBmRNA. Western blotting was used to detect the protein expressions of TRAF6 and NF-κB. CCK-8 assay and flow cytometry were used to detect cell proliferation and apoptosis. Compared with normal chondrocytes, the expression ofmiR-146adecreased, while the mRNA and protein expressions of TRAF6 and NF-κB increased in OA chondrocytes. OA chondrocytes had a lower proliferation rate and a higher apoptosis rate than the normal chondrocytes. Compared with the blank, NC and si-TRAF6 groups, the expression ofmiR-146aincreased in themiR-146amimics group, but decreased in themiR-146ainhibitors andmiR-146ainhibitor + si-TRAF6 groups. Compared with the blank, NC andmiR-146ainhibitor + si-TRAF6 groups, the mRNA and protein expressions of TRAF6 and NF-κB decreased, cell proliferation rate increased and cell apoptosis rate decreased in themiR-146amimics and si-TRAF6 groups, while opposite trends were observed in themiR-146ainhibitors group. Our study suggests thatmiR-146acould promote proliferation and inhibit apoptosis of OA chondrocytes by inhibiting TRAF6 expression and suppressing the activation of NF-κB signalling pathway.
Department of Surgery, Dongying People's Hospital, Dongying 257091, P.R. China.
Jun-Hua
JH
Zhong
Department of Surgery, Dongying People's Hospital, Dongying 257091, P.R. China.
Jing
J
Li
Department of Surgery, Dongying People's Hospital, Dongying 257091, P.R. China.
Cui-Fang
CF
Liu
Department of Surgery, Dongying People's Hospital, Dongying 257091, P.R. China.
Ning
N
Liu
Department of Joint Surgery, Dongying People's Hospital, Dongying 257091, P.R. China.
Rui-Xiang
RX
Bian
Department of Surgery, Dongying People's Hospital, Dongying 257091, P.R. China.
Shou-Mei
SM
Zhao
Department of Joint Surgery, Dongying People's Hospital, Dongying 257091, P.R. China.
Shu-Yi
SY
Yan
Department of Joint Surgery, Dongying People's Hospital, Dongying 257091, P.R. China zhangyb_175@163.com.
Yong-Bing
YB
Zhang
D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D016328:NF-kappa B / Q000032:analysis / Q000235:genetics*; D010003:Osteoarthritis / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000032:analysis / Q000235:genetics*
C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D012333:RNA, Messenger; D048029:TNF Receptor-Associated Factor 6
Chondrocyte; MicroRNA-146a; NF-κB signaling pathway; Osteoarthritis; Proliferation; TRAF6