Multi-substrate biodegradation of chlorophenols by defined microbial consortium.
In the present study, a defined mixed microbial consortium was investigated for their ability to utilize three different monochlorophenols (MCPs) and 2,4-DCP individually and in the mixture. None of the individual strains were able to utilize 3-CP and 4-CP, but when they were mixed to form defined consortium, they have shown great potential and degradation of high concentration of 3-CP and 4-CP. Spectrophotometric analysis of metabolites during MCPs degradation establishes the presence of 2-chloromaleylacetate. Multi-substrate degradation study of 2,4-DCP in the presence of three MCPs showed the great prospect of microbial consortium for in situ bioremediation. During multi-substrate degradation, the biodegradation rate (mg L-1day-1) was observed in the order of 2,4-DCP > 2CP > 3CP > 4CP. Biodegradation kinetic of three MCPs using Andrew's model showed maximum removal rate (Rm) of 2.78, 0.91, 1.82 mg L-1h-1for 2-CP, 3-CP and 4-CP, respectively.
Value Addition, Research and Development Department, National Innovation Foundation-India, Satellite Complex, Jodhpur Tekra, Ahmedabad, 380015, Gujarat, India. bhishmaptl@gmail.com.
Bhishma P
BP
Patel
Environmental Pollution Abatement Lab, Chemical Engineering Department, National Institute of Technology, Rourkela, 769008, Odisha, India.
Arvind
A
Kumar
2,4-Dichlorophenol; Biodegradation; Kinetics; Monochlorophenol; Multi-substrate degradation