Design and synthesis of affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase.
Arachidonic acid (AA) is converted to biologically active metabolites by different pathways, one of the most important of which is initiated by 5-lipoxygenase (5-LO). 5-Hydroxyeicosatetraenoic acid (5-HETE), although possessing only weak biological activity itself, is oxidized to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), a potent chemoattractant for eosinophils and neutrophils. Our main goal is to determine how the biosynthesis of 5-oxo-ETE is regulated and to determine its pathophysiological roles. To achieve this task, we designed and synthesized affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase (5-HEDH), the enzyme responsible for the formation of 5-oxo-ETE.
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Chintam
C
Nagendra Reddy
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Qiuji
Q
Ye
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Pranav
P
Patel
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Sashikala
S
Sivendran
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Shishir
S
Chourey
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Rui
R
Wang
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA.
Jaganmohan R
JR
Anumolu
Meakins-Christie Laboratories, Centre for Translational Biology, McGill University Hospital Centre Research Institute, 1001 Decarie Blvd, Montreal, QC H4A 3J1, Canada.
Gail E
GE
Grant
Meakins-Christie Laboratories, Centre for Translational Biology, McGill University Hospital Centre Research Institute, 1001 Decarie Blvd, Montreal, QC H4A 3J1, Canada.
William S
WS
Powell
Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne FL 32901, USA. Electronic address: jrokach@fit.edu.
Joshua
J
Rokach
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D001095:Arachidonic Acids / Q000378:metabolism; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D008024:Ligands; D009504:Neutrophils / Q000378:metabolism
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D008024:Ligands; C080828:5-oxo-6,8,11,14-eicosatetraenoic acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D000429:Alcohol Oxidoreductases; C099244:5-hydroxyeicosanoid dehydrogenase
5-HEDH; 5-HETE; 5-Oxo-ETE; Affinity chromatography ligand; Asthma; Eosinophil; Neutrophil