Cytogenetic Analysis of Telomere Dysfunction.
Dysfunctional telomeres arising either through natural attrition due to telomerase deficiency or by the removal of telomere-binding proteins are recognized as double-stranded breaks (DSBs). Repair of DSBs is crucial for the maintenance of genome stability. In mammals, DSBs are repaired by either error-prone nonhomologous end joining (NHEJ) or error-free homologous recombination (HR) and can be visualized as chromosomal fusions.
Department of Laboratory Medicine, Yale University School of Medicine, 330 Cedar St., New Haven, CT, 06520, USA.
Rekha
R
Rai
Department of Laboratory Medicine, Yale University School of Medicine, 330 Cedar St., New Haven, CT, 06520, USA.
Asha S
AS
Multani
Department of Laboratory Medicine, Yale University School of Medicine, 330 Cedar St., New Haven, CT, 06520, USA. s.chang@yale.edu.
Sandy
S
Chang
D000818:Animals; D002478:Cells, Cultured; D020732:Cytogenetic Analysis / Q000379:methods; D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D042822:Genomic Instability / Q000235:genetics; D051379:Mice; D019098:Telomerase / Q000235:genetics; D016615:Telomere / Q000235:genetics*
D004268:DNA-Binding Proteins; D019098:Telomerase
Chromosomal fusion; PNA; Telomere FISH; Telomere protection