Determination of Meloxicam in Egg Whites and Yolks Using Reverse Phase Chromatography.
A new method of analysis has been developed and validated for the determination of meloxicam in egg whites and yolks. Following a liquid extraction for the whites and a solid phase extraction for the yolks, samples were separated on an XBridge C18 column and quantified using ultraviolet detection at 360 nm. The mobile phase was a mixture of water with glacial acetic acid and acetonitrile, with a flow rate of 1 mL/min. The procedure produced a linear graph over the concentration range 5-1500 ng/mL with a lower limit of quantification of 5 ng/mL. Intra- and inter-assay variability was 10% or less for both the whites and yolks. The average recovery for whites was 96% and the average recovery in yolks was 97%.
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
Sherry
S
Cox
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
Joan
J
Bailey
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
Molly
M
White
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
Kristen
K
Gordon
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
Marcy
M
Souza
D019342:Acetic Acid; D000097:Acetonitriles; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004529:Egg White / Q000737:chemistry*; D004530:Egg Yolk / Q000737:chemistry*; D016014:Linear Models; D000077239:Meloxicam; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D013843:Thiazines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013844:Thiazoles / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D000097:Acetonitriles; D013843:Thiazines; D013844:Thiazoles; D019342:Acetic Acid; D000077239:Meloxicam; C032159:acetonitrile