Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study.
Adipose-derived stem/stromal cells (ASCs) reside in the stromal vascular fraction (SVF) of adipose tissue (AT) and can be easily isolated. However, extraction of the SVF from lipoaspirate is a critical step in generating ASC, and semiautomated devices have been developed to enhance the efficacy and reproducibility of the outcomes and to decrease manipulation and contamination. In this study, we compared the reference method used in our lab for SVF isolation from lipoaspirate, with three medical devices: GID SVF-1™, Puregraft™, and Stem.pras®. Cell yield and their viability were evaluated as well as their phenotype with flow cytometry. Further on, we determined their proliferative potential using population doublings (PD), PD time (PDT), and clonogenicity assay (CFU-F). Finally, we checked their genetic stability using RT-qPCR for TERT mRNA assay and karyotyping as well as their multilineage potential including adipogenic, chondrogenic, and osteogenic differentiation. Our results demonstrate that all the devices allow the production of SVF cells with consistent yield and viability, in less time than the reference method. Expanded cells from the four methods showed no significant differences in terms of phenotype, proliferation capabilities, differentiation abilities, and genetic stability.
Banque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5 Place d'Arsonval, Pavillon I, 69437 Lyon, France; Lyon University, CarMeN Laboratory, INSERM U1060, 69008 Lyon, France.
Jonathan
0000-0003-3201-3407
J
Rodriguez
Banque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5 Place d'Arsonval, Pavillon I, 69437 Lyon, France.
Anne-Sophie
AS
Pratta
Banque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5 Place d'Arsonval, Pavillon I, 69437 Lyon, France.
Nacira
N
Abbassi
Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR CNRS 5305, Université Lyon 1, Lyon, France; Laboratory for Regenerative Technologies, Department of Biomedical Engineering, University of Basel, 4123 Allschwil, Switzerland.
Hugo
H
Fabre
Banque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5 Place d'Arsonval, Pavillon I, 69437 Lyon, France.
Fanny
F
Rodriguez
Banque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5 Place d'Arsonval, Pavillon I, 69437 Lyon, France.
Cyrille
C
Debard
Laboratoire Central d'Anatomie Pathologique, Hôpital Édouard Herriot, Lyon, France.
Jacqueline
J
Adobati
Department of Plastic, Reconstructive and Aesthetic Surgery, Croix Rousse Hospital, Hospices Civils de Lyon, University of Lyon, UCBL1, Lyon, France.
Fabien
F
Boucher
Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR CNRS 5305, Université Lyon 1, Lyon, France.
Frédéric
F
Mallein-Gerin
Banque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5 Place d'Arsonval, Pavillon I, 69437 Lyon, France.
Céline
C
Auxenfans
Banque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5 Place d'Arsonval, Pavillon I, 69437 Lyon, France.
Odile
O
Damour
Lyon University, CarMeN Laboratory, INSERM U1060, 69008 Lyon, France; Department of Plastic, Reconstructive and Aesthetic Surgery, Croix Rousse Hospital, Hospices Civils de Lyon, University of Lyon, UCBL1, Lyon, France.
Ali
A
Mojallal