Cell-cell and cell-extracellular matrix adhesions cooperate to organize actomyosin networks and maintain force transmission during dorsal closure.
Tissue morphogenesis relies on the coordinated action of actin networks, cell-cell adhesions, and cell-extracellular matrix (ECM) adhesions. Such coordination can be achieved through cross-talk between cell-cell and cell-ECM adhesions.Drosophiladorsal closure (DC), a morphogenetic process in which an extraembryonic tissue called the amnioserosa contracts and ingresses to close a discontinuity in the dorsal epidermis of the embryo, requires both cell-cell and cell-ECM adhesions. However, whether the functions of these two types of adhesions are coordinated during DC is not known. Here we analyzed possible interdependence between cell-cell and cell-ECM adhesions during DC and its effect on the actomyosin network. We find that loss of cell-ECM adhesion results in aberrant distributions of cadherin-mediated adhesions and actin networks in the amnioserosa and subsequent disruption of myosin recruitment and dynamics. Moreover, loss of cell-cell adhesion caused up-regulation of cell-ECM adhesion, leading to reduced cell deformation and force transmission across amnioserosa cells. Our results show how interdependence between cell-cell and cell-ECM adhesions is important in regulating cell behaviors, force generation, and force transmission critical for tissue morphogenesis.
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Katharine
K
Goodwin
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Emily E
EE
Lostchuck
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Kaitlyn M L
KML
Cramb
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada.|Ted Rogers Centre for Heart Research, University of Toronto, Toronto, ON M5G 1M1, Canada.
Teresa
T
Zulueta-Coarasa
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada.|Ted Rogers Centre for Heart Research, University of Toronto, Toronto, ON M5G 1M1, Canada.|Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada.|Developmental and Stem Cell Biology Program, The Hospital for Sick Children, Toronto, ON M5S 1X8, Canada.
Rodrigo
R
Fernandez-Gonzalez
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada tanentz@mail.ubc.ca.
Guy
G
Tanentzapf
D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D021982:Cell-Matrix Junctions / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D047108:Embryonic Development / Q000502:physiology; D004817:Epidermis / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D009218:Myosins / Q000378:metabolism
D000199:Actins; D015820:Cadherins; D029721:Drosophila Proteins; D000205:Actomyosin; D009218:Myosins