Regulation of airway inflammation and remodeling in asthmatic mice by TLR3/TRIF signal pathway.
This paper aims to investigate the effect of Toll-like receptors 3 (TLR3)/TIR-domain-containing adapter-inducing interferon-β (TRIF) signal pathway on the airway inflammation and remodeling in asthmatic mice. C57BL/6 and TLR3-/-mice were randomly divided into three groups (10 mice per group), including Control group (mice inhaled phosphate buffer saline (PBS)), Asthma group (mice inhaled ovalbumin (OVA)) and polyriboinosinic-ribocytidylic acid (poly (I: C)) group (asthmatic mice were injected intraperitoneally with TLR3 agonist poly (I: C)). Hematoxylin-eosin (HE) staining, Wright-Giemsa staining, Enzyme-linked immunosorbent assay (ELISA), Immunohistochemistry, Hydroxyproline assay, quantitative real time polymerase chain reaction (qRT-PCR) and Western blot were used to assess for the indices of airway inflammation and remodeling. In terms of WT mice, all asthma groups with or without the addition of poly (I: C) showed exaggerated inflammation and remodeling in the airways as compared to Control group, which were more seriously in poly (I: C) group than Asthma group. Furthermore, we observed the significant inhibition of airway inflammation and remodeling in the TLR3-/-mice in both Asthma no matter with or without addition of poly (I: C) than the WT mice. TLR3 knockout could obviously relieve the airway inflammation and remodeling in asthma through inhibiting TLR3/TRIF signaling pathway.
Department of critical care medicine, The Third People's Hospital of Jinan, Jinan 250132, Shandong, PR China. Electronic address: yangmeiyyy@sina.com.
Mei
M
Yang
Department of critical care medicine, The Third People's Hospital of Jinan, Jinan 250132, Shandong, PR China.
Hao-Ying
HY
Wang
Department of emergency, Shandong Provincial Western Hospital, Jinan 250021, Shandong, PR China.
Jian-Chang
JC
Chen
Department of cardiology, Qilu Hospital Affiliated to Shandong University, Jinan 250012, PR China.
Jing
J
Zhao
D033942:Adaptor Proteins, Vesicular Transport / Q000276:immunology* / Q000378:metabolism; D056151:Airway Remodeling / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011014:Pneumonia / Q000276:immunology / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology; D051196:Toll-Like Receptor 3 / Q000276:immunology* / Q000378:metabolism
D033942:Adaptor Proteins, Vesicular Transport; D051196:Toll-Like Receptor 3; C476286:Ticam1 protein, mouse; C519703:TLR3 protein, mouse
Airway inflammation; Airway remodeling; Asthma; TLR3; TRIF