The miR-24-3p/p130Cas: a novel axis regulating the migration and invasion of cancer cells.
MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression by suppressing translation or facilitating mRNA decay. Differential expression of miRNAs is involved in the pathogenesis of several diseases including cancer. Here, we investigated the role of-miR-24-3p as a downregulated miRNA in metastatic cancer. miR-24-3p was decreased in metastatic cancer and lower expression of miR-24-3p was related to poor survival of cancer patients. Consistently, ectopic expression of miR-24-3p suppressed the cell migration, invasion, and proliferation of MCF7, Hep3B, B16F10, SK-Hep1, and PC-3 cells by directly targeting p130Cas. Stable expression of p130Cas restored miR-24-3p-mediated inhibition of cell migration and invasion. These results suggest that miR-24-3p functions as a tumor suppressor and the miR-24-3p/p130Cas axis is a novel factor of cancer progression by regulating cell migration and invasion.
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Hoin
H
Kang
Department of Molecular Science and Technology, Ajou University, Suwon, South Korea.
Jun Gi
JG
Rho
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Chongtae
C
Kim
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Hyosun
H
Tak
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Heejin
H
Lee
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Eunbyul
E
Ji
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Sojin
S
Ahn
Catholic Cancer Research Institute, College of Medicine, The Catholic University of Korea, South Korea.
A-Ri
AR
Shin
Catholic Cancer Research Institute, College of Medicine, The Catholic University of Korea, South Korea.
Hyun-Il
HI
Cho
Department of Life Science, Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, South Korea.
Yun Hyun
YH
Huh
Department of Life Science, Bio Imaging and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, South Korea.
Woo Keun
WK
Song
Department of Molecular Science and Technology, Ajou University, Suwon, South Korea.
Wook
W
Kim
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.|Cancer Evolution Research Center, The Catholic University of Korea, Seoul, South Korea.
Eun Kyung
EK
Lee
D020413:3' Untranslated Regions; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D050719:Crk-Associated Substrate Protein / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality; D034622:RNA Interference; D059467:Transcriptome; D023041:Xenograft Model Antitumor Assays
D020413:3' Untranslated Regions; C493841:BCAR1 protein, human; D050719:Crk-Associated Substrate Protein; C543845:MIRN24 microRNA, human; D035683:MicroRNAs