Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection.
Distinct Trypanosoma cruzi genotypes have been considered relevant for patient management and therapeutic response of Chagas disease. However, typing strategies for genotype-specific serodiagnosis of Chagas disease are still unavailable and requires standardization for practical application. In this study, an innovative TcI/TcVI/TcII Chagas Flow ATE-IgG2a technique was developed with applicability for universal and genotype-specific diagnosis of T. cruzi infection. For this purpose, the reactivity of serum samples (percentage of positive fluorescent parasites-PPFP) obtained from mice chronically infected with TcI/Colombiana, TcVI/CL or TcII/Y strain as well as non-infected controls were determined using amastigote-AMA, trypomastigote-TRYPO and epimastigote-EPI in parallel batches of TcI, TcVI and TcII target antigens. Data demonstrated that "α-TcII-TRYPO/1:500, cut-off/PPFP = 20%" presented an excellent performance for universal diagnosis of T. cruzi infection (AUC = 1.0, Se and Sp = 100%). The combined set of attributes "α-TcI-TRYPO/1:4,000, cut-off/PPFP = 50%", "α-TcII-AMA/1:1,000, cut-off/PPFP = 40%" and "α-TcVI-EPI/1:1,000, cut-off/PPFP = 45%" showed good performance to segregate infections with TcI/Colombiana, TcVI/CL or TcII/Y strain. Overall, hosts infected with TcI/Colombiana and TcII/Y strains displayed opposite patterns of reactivity with "α-TcI TRYPO" and "α-TcII AMA". Hosts infected with TcVI/CL strain showed a typical interweaved distribution pattern. The method presented a good performance for genotype-specific diagnosis, with global accuracy of 69% when the population/prototype scenario include TcI, TcVI and TcII infections and 94% when comprise only TcI and TcII infections. This study also proposes a receiver operating reactivity panel, providing a feasible tool to classify serum samples from hosts infected with distinct T. cruzi genotypes, supporting the potential of this method for universal and genotype-specific diagnosis of T. cruzi infection.
Laboratório de Doença de Chagas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Instituto de Ciências Exatas e Biológicas (ICEB), Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil.
Glaucia Diniz
GD
Alessio
Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil.
Fernanda Fortes
FF
de Araújo
Laboratório de Doença de Chagas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Instituto de Ciências Exatas e Biológicas (ICEB), Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil.
Denise Fonseca
DF
Côrtes
Grupo de Genômica Funcional e Proteômica de Leishmania spp e Trypanosoma cruzi, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil.
Policarpo Ademar
PA
Sales Júnior
Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil.
Daniela Cristina
DC
Lima
Laboratório de Bioinformática e Análises Moleculares, Universidade Federal de Uberlândia, INGEB/FACOM, Campus Patos de Minas, Patos de Minas, Minas Gerais, Brazil.
Matheus de Souza
MS
Gomes
Laboratório de Bioinformática e Análises Moleculares, Universidade Federal de Uberlândia, INGEB/FACOM, Campus Patos de Minas, Patos de Minas, Minas Gerais, Brazil.
Laurence Rodrigues
LR
do Amaral
Grupo de Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil.
Marcelo Antônio Pascoal
MA
Xavier
Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil.
Andréa
A
Teixeira-Carvalho
Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil.
Olindo Assis
OA
Martins-Filho
Laboratório de Doença de Chagas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Instituto de Ciências Exatas e Biológicas (ICEB), Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil.
Marta
M
de Lana
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000175:diagnosis*; D005260:Female; D005838:Genotype; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D012372:ROC Curve; D012044:Regression Analysis; D012698:Serologic Tests / Q000379:methods*; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000276:immunology
D000953:Antigens, Protozoan; D007074:Immunoglobulin G