Assessment of Mitophagy in iPS Cell-Derived Neurons.
Aberrant mitochondrial function is associated with many neurological diseases. Mitophagy is a key mechanism for the elimination of damaged mitochondria and maintenance of mitochondrial homeostasis. Induced pluripotent stem (iPS) cell technologies developed over the last decade have allowed us to analyze functions of the human neuron. Here we describe an efficient induction method from human iPS cells to neurons, followed by an image-based mitophagy assay.
Department of Neurology, Juntendo University School of Medicine, Bunkyo, Tokyo, 113-8421, Japan.
Kei-Ichi
KI
Ishikawa
Center for Genomic and Regenerative Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo, Tokyo, 113-8421, Japan.
Akihiro
A
Yamaguchi
Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, 160-8582, Japan.
Hideyuki
H
Okano
Center for Genomic and Regenerative Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo, Tokyo, 113-8421, Japan. awado@juntendo.ac.jp.
Wado
W
Akamatsu
D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D063306:Mitophagy; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism
Autophagy; Mitophagy; Neurons; Parkinson’s disease; iPS cells