pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity.
Experimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as a model to study multiple sclerosis immunopathology. EAE has also been extensively employed to evaluate potentially therapeutic schemes. Considering the presence of an immune response directed to heat shock proteins (hsps) in autoimmune diseases and the immunoregulatory potential of these molecules, we evaluated the effect of a previous immunization with a genetic vaccine containing the mycobacterial hsp65 gene on EAE development. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and 14 days later were submitted to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG35-55) emulsified in Complete Freund's Adjuvant. Vaccinated mice presented significant lower clinical scores and lost less body weight. MOG35-55immunization also determined less inflammation in lumbar spinal cord but did not change CD4+CD25+Foxp3+ T cells frequency in spleen and CNS. Infiltrating cells from the CNS stimulated with rhsp65 produced significantly higher levels of IL-10. These results suggest that the ability of pVAXhsp65 vaccination to control EAE development is associated with IL-10 induction.
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Sofia Fernanda Gonçalves
0000-0001-9030-0768
SF
Zorzella-Pezavento
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Fernanda
F
Chiuso-Minicucci
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Thais Graziela Donegá
0000-0001-8307-1311
TG
França
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Larissa Lumi Watanabe
0000-0003-3481-2181
LL
Ishikawa
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Larissa Camargo
0000-0002-4849-8835
LC
da Rosa
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Priscila Maria
0000-0002-1619-6728
PM
Colavite
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Bianca
0000-0002-0676-3012
B
Balbino
Flow Cytometry Laboratory, Amaral Carvalho Foundation, Jaú, SP, Brazil.
Camila
C
Marques
Flow Cytometry Laboratory, Amaral Carvalho Foundation, Jaú, SP, Brazil.
Maura Rosane Valerio
MR
Ikoma
Department of Biochemistry and Immunology, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.
Ana Paula
AP
Masson
Department of Biochemistry and Immunology, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.
Célio Lopes
CL
Silva
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Alexandrina
0000-0003-4557-3331
A
Sartori
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D018834:Chaperonin 60 / Q000235:genetics*; D003001:Cloning, Molecular; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000517:prevention & control; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000276:immunology* / Q000517:prevention & control; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology; D009187:Myelitis / Q000276:immunology* / Q000517:prevention & control; D010446:Peptide Fragments / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D001426:Bacterial Proteins; D018834:Chaperonin 60; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D019444:Vaccines, DNA; C074119:heat-shock protein 65, Mycobacterium; C445081:myelin oligodendrocyte glycoprotein (35-55)