Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production.
A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.
Bioprospecting Group, Agharkar Research Institute , Pune, India.
Gulshan R
GR
Walke
Bioprospecting Group, Agharkar Research Institute , Pune, India.
Dnyanesh S
DS
Ranade
Bioprospecting Group, Agharkar Research Institute , Pune, India.
Shefali N
SN
Ramteke
Proteomics Laboratory, National Centre for Cell Science (NCCS), University of Pune Campus , Pune, India.
Srikanth
S
Rapole
Department of Chemical Sciences, University of Catania , Viale Andrea Doria 6, 95125 Catania, Italy.|Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici , via Celso Ulpiani, 27, 70125 Bari, Italy.
Cristina
http://orcid.org/0000-0001-5348-5863
C
Satriano
Department of Chemical Sciences, University of Catania , Viale Andrea Doria 6, 95125 Catania, Italy.|Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici , via Celso Ulpiani, 27, 70125 Bari, Italy.|Institute of Biostructures and Bioimages, National Council of Research , Via P. Gaifami 18, 95126 Catania, Italy.
Enrico
E
Rizzarelli
Department of Chemical Sciences, University of Catania , Viale Andrea Doria 6, 95125 Catania, Italy.
Gaetano A
http://orcid.org/0000-0002-6146-6934
GA
Tomaselli
Department of Chemical Sciences, University of Catania , Viale Andrea Doria 6, 95125 Catania, Italy.
Giuseppe
G
Trusso Sfrazzetto
Bioprospecting Group, Agharkar Research Institute , Pune, India.
Prasad P
http://orcid.org/0000-0002-3929-2990
PP
Kulkarni
D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology*; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
D016229:Amyloid beta-Peptides; D002614:Chelating Agents; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; C433241:amyloid beta-protein (1-16); C075222:amyloid beta-protein (1-42); D003300:Copper