HIV-1 susceptibility of transgenic rat-derived primary macrophage/T cells and a T cell line that express human receptors, CyclinT1 and CRM1 genes.
We developed transgenic (Tg) rats that express human CD4, CCR5, CXCR4, CyclinT1, and CRM1 genes. Tg rat macrophages were efficiently infected with HIV-1 and supported production of infectious progeny virus. By contrast, both rat primary CD4+T cells and established T cell lines expressing human CD4, CCR5, CyclinT1, and CRM1 genes were infected inefficiently, but this was ameliorated by inhibition of cyclophilin A. The infectivity of rat T cell-derived virus was lower than that of human T cell-derived virus.
Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
Hisatoshi
http://orcid.org/0000-0001-9061-7327
H
Shida
Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
Hiroyuki
H
Okada
Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
Hajime
H
Suzuki
Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
Xianfeng
X
Zhang
Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, 060-0815, Japan.
Jing
J
Chen
Department of Medical Technology, Tokyo University of Technology, 5-23-22 Nishikamata, Ohta-ku, Tokyo, 144-8535, Japan.
Yasuko
Y
Tsunetsugu-Yokota
Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa, 903-0125, Japan.
Yuetsu
Y
Tanaka
Department of Medicinal Chemistry, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Fumika
F
Yakushiji
Department of Medicinal Chemistry, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Yoshio
Y
Hayashi
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D002460:Cell Line; D002478:Cells, Cultured; D056906:Cyclin T / Q000235:genetics / Q000378:metabolism*; D004198:Disease Susceptibility; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000472:pathogenicity; D006801:Humans; D028884:Karyopherins / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000821:virology; D051381:Rats; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D000097606:Exportin 1 Protein
D056906:Cyclin T; D028884:Karyopherins; D019718:Receptors, CXCR4; D018160:Receptors, Cytoplasmic and Nuclear; D000097606:Exportin 1 Protein