Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein-Protein Interactions.
Studying protein-protein interactions is critical to our understanding of signaling pathways. Telomere Interactome is assembled around telomeres and consists of proteins and factors from diverse pathways. Dissecting how this protein network contributes to telomere protection and length regulation requires the elucidation of the complex and dynamic interactions between the proteins within the interactome. Here, we focus on three assays, Bi-molecular Fluorescence Complementation (BiFC), Gaussia Luciferase Protein-fragment Complementation Assay (GLuc PCA), and OPAL peptide array, which have proven vital in our studies of telomere protein interaction networks.
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, 132 East Waihuan Rd., Higher Education Mega Center, Guangzhou, People's Republic of China.
Wenbin
W
Ma
Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea.|Severance Integrative Research Institute for Cerebral and Cardiovascular Diseases, Yonsei University Health System, Seoul, Republic of Korea.
Ok-Hee
OH
Lee
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Hyeung
H
Kim
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA. songyang@bcm.edu.
Zhou
Z
Songyang
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005453:Fluorescence; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D025941:Protein Interaction Mapping / Q000379:methods; D015398:Signal Transduction / Q000502:physiology; D016615:Telomere / Q000378:metabolism*
D008164:Luminescent Proteins; D010455:Peptides; D008156:Luciferases
BiFC; GLuc PCA; Peptide array; Protein fragment complementation (PCA); Protein-protein interaction; Telomere; Telomere interactome