Antiproliferation effect of guanosine on HCT 116 cells involves MAPK and AMPK pathways.
This study aims to investigate the mechanisms associated with the antiproliferation effect of guanosine on human colon carcinoma HCT 116 cells. In this study, guanosine induced more drastic cell cycle arrest effect than cell death effect on HCT 116 cells. The cell cycle arrest effect of guanosine on HCT 116 cells appeared to be associated with the increased activation of mitogen-activated protein kinases (MAPK) such as ERK1/2, p38 and JNK. The decrease of AMP-activated protein kinase (AMPK) activation and cyclin D1 expression was also involved. Thus, the antiproliferation of colon cancer cells of guanosine could be mediated by the disruption of MAPK and AMPK pathways.
a Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
Wuen Yew
WY
Teoh
b Biology Division , Centre for Foundation Studies in Science, University of Malaya , Kuala Lumpur , Malaysia.
Norhanom Abdul
NA
Wahab
a Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
Kae Shin
KS
Sim
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006151:Guanosine / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism* / Q000493:pharmacokinetics; D010766:Phosphorylation / Q000187:drug effects
D000970:Antineoplastic Agents; D006151:Guanosine; D019938:Cyclin D1; D020928:Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases
Guanosine; HCT 116 cells; cell cycle; cell signalling