Identification of genotoxic compounds in crude oil using fractionation according to distillation, polarity and Kow.
We examined the degree of DNA damage caused by fractions of crude oil in accordance with the boiling points, polarity and log Kow. Relatively high DNA damage was observed in the aromatic fraction (290-330°C) and resin and polar fraction (350-400°C). The resin and polar fraction showed relatively high genotoxicity compared with the aliphatic and aromatic fraction at the 1-4 log Kowrange. At the 6-7 log Kowrange, the aromatic fraction showed relatively high DNA damage compared with the aliphatic and resin and polar fraction. In particular, every detailed fraction in accordance with the log Kowvalues (aliphatic and aromatic (310-320°C) and resins and polar fractions (370-380°C)) showed one or less than one DNA damage. However, the fractions before separation in accordance with log Kowvalues (aliphatic and aromatic (310-320°C) and resin and polar (370-380°C) fractions) showed high DNA damage. Thus, we confirm the synergistic action between the detailed compounds.
Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea.
Shin Yeong
SY
Park
Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea; Institute of Marine Industry College of Marine Science, Gyeongsang National University, Tongyeong, Republic of Korea.
Hyo Jin
HJ
Lee
School of Earth and Environmental Sciences, Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
Jong Seong
JS
Khim
Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea; Institute of Marine Industry College of Marine Science, Gyeongsang National University, Tongyeong, Republic of Korea. Electronic address: kgb@gnu.ac.kr.
Gi Beum
GB
Kim
D000818:Animals; D005591:Chemical Fractionation; D004249:DNA Damage; D057168:Distillation; D005432:Flounder; D010578:Petroleum
D010578:Petroleum
Boiling point; Comet assay; Crude oil; Fractionation; Log K(ow); Polarity