Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging.
Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.
Department of Genetics, Albert Einstein College of Medicine, Bronx, New York.
Hwa Jin
HJ
Jung
Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon.|Department of Biomolecular Science.
Kwang-Pyo
KP
Lee
Department of Genetics, Albert Einstein College of Medicine, Bronx, New York.
Brandon
B
Milholland
Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon.|Department of Functional Genomics, Korea University of Science and Technology, Daejeon.
Yeo Jin
YJ
Shin
Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon.|Department of Functional Genomics, Korea University of Science and Technology, Daejeon.
Jae Sook
JS
Kang
Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon.|Department of Functional Genomics, Korea University of Science and Technology, Daejeon.
Ki-Sun
KS
Kwon
Department of Genetics, Albert Einstein College of Medicine, Bronx, New York.
Yousin
Y
Suh
D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009133:Muscular Atrophy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012313:RNA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D015415:Biomarkers; D035683:MicroRNAs; D012313:RNA
Disuse muscle atrophy; MicroRNA; Muscle aging; Sarcopenia; Serum