Structural and genetic characterization of the O-antigen of Enterobacter cloacae C5529 related to the O-antigen of E. cloacae G3054.
On mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C5529, the O-polysaccharide chain was cleaved at the linkages of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Psep5Ac7Ac). The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with1H and13C NMR spectroscopy, and the following structure of the tetrasaccharide repeating unit of the O-polysaccharide was established: →4)-β-Psep5Ac7Ac-(2 → 3)-β-d-Galp-(1 → 6)-β-d-Galf-(1 → 3)-α-d-Galp-(1→ It differs from a structurally related O-polysaccharide of E. cloacae G3045 studied early (Perepelov, A. V.; Wang, M.; Filatov, A. V.; Guo, X.; Shashkov, A. S.; Wang, L.; Knirel, Y. A. Carbohydr. Res. 2015; 407:59-62) in positions of substitution of β-Psep5Ac7Ac (O-4 vs. O-8) and β-Galp (O-3 vs. O-6) and the absence of a side-chain α-Galp residue. The O-antigen gene clusters of E. cloacae C5529 and G3045 are organized identically and include genes with the same putative functions in the O-polysaccharide synthesis. Based on these and serological data, it is suggested to combine E. cloacae C5529 and G3054 in one O-serogroup as two subgroups.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Runhua
R
Han
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
Andrei V
AV
Perepelov
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Yuanyuan
Y
Wang
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Andrei V
AV
Filatov
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Min
M
Wang
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Alexander S
AS
Shashkov
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China; Tianjin Biochip Corporation, TEDA, Tianjin, 300457, China.
Lei
L
Wang
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Yuriy A
YA
Knirel
D002240:Carbohydrate Sequence; D016972:Enterobacter cloacae / Q000737:chemistry* / Q000235:genetics*; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*
D019081:O Antigens
Bacterial polysaccharide structure; Enterobacter cloacae; O-antigen; O-antigen gene cluster; Pseudaminic acid