Stilbene content and expression of stilbene synthase genes in cell cultures of Vitis amurensis treated with cinnamic and caffeic acids.
It has previously been shown that exogenous application of p-coumaric acid (CA), a precursor of phenolic compounds, improved stilbene production in cell cultures of Vitis amurensis. This study examines the effect of cinnamic (Cin) and caffeic (Caf) acids, which are also phenolic precursors, on stilbene biosynthesis in the cell cultures. Five stilbenes, t-resveratrol diglucoside, t-piceid (t-resveratrol glucoside), t-resveratrol, t-ε-viniferin, and t-δ-viniferin, were found in the treated and untreated cells. Cin acid increased the total stilbene production in the grape cell cultures 2.3-3.5 times in comparison with that in the untreated cells. Caf acid increased the total stilbene production by 1.8- to 1.9-fold, but this increase was not considerably different from stilbene production in the untreated cells. Cin acid affected the total stilbene production via a marked increase in the content of t-resveratrol diglucoside (up to 2.2 times), t-piceid (up to three times), t-resveratrol (up to 5.1 times), t-ε-viniferin (up to eight times), and t-δ-viniferin (up to 9.2 times). Transcription levels of VaSTS5, 6, 7, 8, and 10 genes considerably increased under 0.1, 0.25, and 0.5 mM Cin acid. These results indicate that Cin acid increased stilbene production in V. amurensis calli via a selective enhancement of STS gene expression.
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
Alexey P
AP
Tyunin
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.|Department of Biochemistry and Biotechnology, Far Eastern Federal University, Vladivostok, Russia.
Nikolay N
NN
Nityagovsky
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
Valeria P
VP
Grigorchuk
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.|Department of Biochemistry and Biotechnology, Far Eastern Federal University, Vladivostok, Russia.
Konstantin V
KV
Kiselev
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D002109:Caffeic Acids / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002934:Cinnamates / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D013267:Stilbenes / Q000032:analysis / Q000378:metabolism*; D027843:Vitis / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D002109:Caffeic Acids; D002934:Cinnamates; D010940:Plant Proteins; D013267:Stilbenes; C029010:cinnamic acid; D000217:Acyltransferases; C022674:stilbene synthase; C040048:caffeic acid
caffeic acid; callus culture; cinnamic acid; piceid; resveratrol; stilbene precursors; stilbene synthase; stilbenes; viniferins, Vitis amurensis