Neuroprotective Role of Exogenous Brain-Derived Neurotrophic Factor in Hypoxia-Hypoglycemia-Induced Hippocampal Neuron Injury via Regulating Trkb/MiR134 Signaling.
Hypoxic-ischemic brain injury is an important cause of neonatal mortality and morbidity. Brain-derived neurotrophic factor (BDNF) has been reported to play a neuroprotective role in hypoxic-ischemic brain injury; however, the specific effects and mechanism of BDNF on hypoxic-hypoglycemic hippocampal neuron injury remains unknown. The current study investigated the action of BDNF in regulating cerebral hypoxic-ischemic injury by simulating hippocampal neuron ischemia and hypoxia. We found that BDNF, p-Trkb, and miR-134 expression levels decreased, and that exogenous BDNF increased survival and reduced apoptosis in hypoxic-hypoglycemic hippocampal neurons. The results also show that BDNF inhibits MiR-134 expression by activating the TrkB pathway. Transfection with TrkB siRNA and pre-miR-134 abrogated the neuroprotective role of BDNF in hypoxic-hypoglycemic hippocampal neurons. Our results suggest that exogenous BDNF alleviates hypoxic-ischemic brain injury through the Trkb/MiR-134 pathway. These findings may help to identify a potential therapeutic agent for the treatment of hypoxic-ischemic brain injury.
Department of Neurosurgery, Shaanxi Provincial People's Hospital, NO 256, Youyi West Road, Xi'an, 710068, China. weidonghuangsx@163.com.
Weidong
W
Huang
Department of Neurosurgery, Shaanxi Provincial People's Hospital, NO 256, Youyi West Road, Xi'an, 710068, China.
Facai
F
Meng
Department of Neurosurgery, Shaanxi Provincial People's Hospital, NO 256, Youyi West Road, Xi'an, 710068, China.
Jie
J
Cao
Department of Neurosurgery, Shaanxi Provincial People's Hospital, NO 256, Youyi West Road, Xi'an, 710068, China.
Xiaobin
X
Liu
Department of Neurosurgery, Shaanxi Provincial People's Hospital, NO 256, Youyi West Road, Xi'an, 710068, China.
Jie
J
Zhang
Department of Neurosurgery, Shaanxi Provincial People's Hospital, NO 256, Youyi West Road, Xi'an, 710068, China.
Min
M
Li
D000818:Animals; D017209:Apoptosis; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005947:Glucose / Q000172:deficiency; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D020813:Receptor, trkB / Q000378:metabolism*; D015398:Signal Transduction
D019208:Brain-Derived Neurotrophic Factor; C581995:MIRN134 microRNA, rat; D035683:MicroRNAs; D018696:Neuroprotective Agents; C000621230:Ntrk2 protein, rat; D020813:Receptor, trkB; D005947:Glucose; D010100:Oxygen
BDNF; Hypoxic–ischemic brain injury; TrkB; hippocampal neuron; miR-134