Highly Emissive Dye-Sensitized Upconversion Nanostructure for Dual-Photosensitizer Photodynamic Therapy and Bioimaging.
Rare-earth-based upconversion nanotechnology has recently shown great promise for photodynamic therapy (PDT). However, the NIR-induced PDT is greatly restricted by overheating issues on normal bodies and low yields of reactive oxygen species (ROS,1O2). Here, IR-808-sensitized upconversion nanoparticles (NaGdF4:Yb,Er@NaGdF4:Nd,Yb) were combined with mesoporous silica, which has Ce6 (red-light-excited photosensitizer) and MC540 (green-light-excited photosensitizer) loaded inside through covalent bond and electrostatic interaction, respectively. When irradiated by tissue-penetrable 808 nm light, the IR-808 greatly absorb 808 nm photons and then emit a broadband peak which overlaps perfectly with the absorption of Nd3+and Yb3+. Thereafter, the Nd3+/Yb3+incorporated shell synergistically captures the emitted NIR photons to illuminate NaGdF4:Yb,Er zone and then radiate ultrabright green and red emissions. The visible emissions simultaneously activate the dual-photosensitizer to produce a large amount of ROS and, importantly, low heating effects. The in vitro and in vivo experiments indicate that the dual-photosensitizer nanostructure has trimodal (UCL/CT/MRI) imaging functions and high anticancer effectiveness, suggesting its potential clinical application as an imaging-guided PDT technique.
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Jiating
J
Xu
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Piaoping
0000-0002-9555-1803
P
Yang
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Mingdi
M
Sun
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Huiting
H
Bi
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Bin
B
Liu
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Dan
D
Yang
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Shili
S
Gai
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University , Harbin 150001, P. R. China.
Fei
F
He
State Key Laboratory of Rare Earth Resource Utilization, Changhcun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.
Jun
0000-0001-9572-2134
J
Lin
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D005260:Female; D006801:Humans; D008027:Light; D047028:Macrocyclic Compounds / Q000008:administration & dosage / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D008674:Metals, Rare Earth / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D064847:Multimodal Imaging / Q000379:methods; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000628:therapy*; D061848:Optical Imaging / Q000379:methods; D010316:Particle Size; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry; D016062:Porosity; D011744:Pyrimidinones / Q000008:administration & dosage / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D014057:Tomography, X-Ray Computed / Q000379:methods
D000970:Antineoplastic Agents; D003287:Contrast Media; D047028:Macrocyclic Compounds; D008674:Metals, Rare Earth; D017319:Photosensitizing Agents; D011744:Pyrimidinones; C003954:merocyanine dye; D012822:Silicon Dioxide
IR-808; bioimaging; dual-photosensitizer; photodynamic therapy; upconversion