A gold nanoparticle-mediated rapid in vitro assay of anti-aggregation reagents for amyloid β and its validation.
Herein, we report a rapid in vitro assay of amyloid β anti-aggregation reagents using gold nanoparticles as nucleation cores and optical reporters. Based on the colorimetric responses observed with the naked eye, effective anti-aggregation reagents were rapidly (<15 min) distinguished. For these reagents, conventional methods showed fewer amyloid β aggregates and an increased viability of neuroblastoma cells after treatment. We envision that our proposed method will facilitate the discovery of efficient drug components for the treatment of Alzheimer's disease.
Nanobiointerface Laboratory, Department of Life Science, University of Seoul, Dongdaemun-ku, Seoul 130-743, Republic of Korea. inheechoi1@uos.ac.kr.
Hye Young
HY
Kim
Donghee
D
Lee
Kwon-Yul
KY
Ryu
Inhee
I
Choi