Postprandial triglyceride-rich lipoproteins promote lipid accumulation and apolipoprotein B-48 receptor transcriptional activity in human circulating and murine bone marrow neutrophils in a fatty acid-dependent manner.
Postprandial triglyceride-rich lipoproteins (TRLs) promote atherosclerosis. Recent research points the bone marrow (BM) as a primary site in atherosclerosis. We elucidated how the acute administration of monounsaturated fatty acids (MUFAs) MUFAs, omega-3 polyunsaturated fatty acids (PUFAs) PUFAs and saturated fatty acids (SFAs) affects human circulating and murine BM neutrophil lipid accumulation and functionality.



Postprandial hypertriglyceridemia was induced in healthy subjects and Apoe-/-mice by the acute administration of dietary fats enriched in MUFAs, PUFAs, or SFAs. Postprandial hypertriglyceridemia increased apolipoprotein-B48 receptor (ApoB48R) transcriptional activity that was linearly correlated with intracellular triglycerides (TGs) TGs accumulation in human circulating and murine BM neutrophils. MUFA and omega-3 PUFAs attenuated ApoB48R gene expression and intracellular TG accumulation compared to SFAs. TRLs induced apoB48R-dependent TG accumulation in human neutrophils ex vivo. Murine BM neutrophils showed a decrease in surface L-selectin and an increase in TNF-α and IL-1β mRNA expressions only after SFAs administration. TRLs enriched in SFAs induced BM neutrophil degranulation ex vivo suggesting cell priming/activation.



Postprandial TRLs disrupts the normal biology and function of circulating and BM neutrophils. MUFA- and omega-3 PUFA-rich dietary fats such as virgin olive oil or fish oil has the potential to prevent excessive neutrophil lipid accumulation and activation by targeting the fatty acid composition of TRLs.
Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
Almudena
A
Ortega-Gómez
Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
Lourdes M
LM
Varela
Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
Sergio
S
López
Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
Sergio
S
Montserrat de la Paz
Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
Rosario
R
Sánchez
Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
Francisco J G
FJG
Muriana
Department of Cell Biology, University of Seville, Sevilla, Spain (B.B).
Beatriz
0000-0002-7429-6567
B
Bermúdez
Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.
Rocío
R
Abia
D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D005227:Fatty Acids / Q000008:administration & dosage; D006801:Humans; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000378:metabolism*; D019518:Postprandial Period; D018110:Receptors, Lipoprotein / Q000235:genetics*; D014158:Transcription, Genetic; D014280:Triglycerides / Q000502:physiology*
D004041:Dietary Fats; D005227:Fatty Acids; D008074:Lipoproteins; D018110:Receptors, Lipoprotein; D014280:Triglycerides; C411291:apo B48 receptor; C030087:lipoprotein triglyceride
ApoB48R; Atherosclerosis; Bone marrow; MUFAs; Neutrophils; Omega-3 PUFAs; Postprandial triglyceride-rich lipoproteins; Saturated fatty acids