Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count.
Background.Dendritic cells could be involved in immune surveillance of highly immunogenic tumors such as melanoma. Their role in the progression melanocytic nevi to melanoma is however a matter of controversy.Methods.The number of dendritic cells within epidermis, in peritumoral zone, and within the lesion was counted on slides immunohistochemically stained for CD1a, CD1c, DC-LAMP, and DC-SIGN in 21 of dysplastic nevi, 27 in situ melanomas, and 21 invasive melanomas.Results.We found a significant difference in the density of intraepidermal CD1c+ cells between the examined lesions; the mean CD1c cell count was 7.00/mm2for invasive melanomas, 2.94 for in situ melanomas, and 13.35 for dysplastic nevi. The differences between dysplastic nevi and melanoma in situ as well as between dysplastic nevi and invasive melanoma were significant. There was no correlation in number of positively stained cells between epidermis and dermis. We did not observe any intraepidermal DC-LAMP+ cells neither in melanoma in situ nor in invasive melanoma as well as any intraepidermal DC-SIGN+ cells in dysplastic nevi.Conclusion.It was shown that the number of dendritic cells differs between dysplastic nevi, in situ melanomas, and invasive melanomas. This could eventually suggest their participation in the development of melanoma.
Chair of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, Grzegórzecka 16, 31-351 Kraków, Poland.
Grzegorz
G
Dyduch
II Chair of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Skawińska 8, 31-066 Kraków, Poland.
Katarzyna Ewa
KE
Tyrak
Chair of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, Grzegórzecka 16, 31-351 Kraków, Poland.
Anna
A
Glajcar
Chair of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, Grzegórzecka 16, 31-351 Kraków, Poland.
Joanna
J
Szpor
Chair of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, Grzegórzecka 16, 31-351 Kraków, Poland.
Magdalena
0000-0001-7233-2213
M
Ulatowska-Białas
Chair of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, Grzegórzecka 16, 31-351 Kraków, Poland.
Krzysztof
0000-0002-1921-5007
K
Okoń
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018949:Antigens, CD1 / Q000378:metabolism*; D002452:Cell Count; D002648:Child; D003713:Dendritic Cells / Q000473:pathology*; D004416:Dysplastic Nevus Syndrome / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008545:Melanoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged
D018949:Antigens, CD1; C437944:CD1C protein, human; D006023:Glycoproteins