Phillyrin lowers body weight in obese mice via the modulation of PPAR<beta>/<delta>-ANGPTL 4 pathway.
Previous investigations have shown that the peroxisome proliferator activated receptor beta/delta (PPAR<beta>/<delta>)-angiopoietin-like protein 4 (ANGPTL4) pathways may be a new pharmacologic target for treatment of obesity. The present study was conducted to test the effect of phillyrin, ‎a glucoside, on obesity in mice.



Fifty mice were randomly divided into 5 groups (n=10): control group (C57BL/6J mice), obese mice group, two groups of obese mice treated with phillyrin (15 or 45mg/kg/day), one group of obese mice treated with PPAR<beta>/<delta> agonist GW0742 (3mg/kg/day). Twelve weeks after treatment, body weight, liver weight, fat weight, lipid levels in the liver, serum levels of tumour necrosis factor-<alpha>(TNF-<alpha>), leptin, and insulin, expression of PPAR<beta>/<delta>, ANGPTL4, and AMP-activated protein kinase (AMPK) were determined.



Treatment with phillyrin (15 or 45mg/kg) significantly decreased body weight, liver weight, fat weight, hepatic total cholesterol, free fatty acid, and triglyceride concentrations, serum levels of TNF-<alpha>, leptin, and insulin concomitantly with up-regulated expression of PPAR<beta>/<delta>, ANGPTL4, and p-AMPK-<alpha>. In addition, GW0742 has similar effect of phillyrin.



The present results suggest that phillyrin could regulate the PPAR<beta>/<delta>-ANGPTL 4 pathway to lower body weight in obese C57BL/6J mice.
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. Electronic address: xhbzhb@yahoo.com.
Hong-Bo
HB
Xiao
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
Guo-Guang
GG
Sui
Hunan Province University Key Laboratory for Agricultural Biochemistry and Biotransformation, Hunan Agricultural University, Changsha 410128, China; Hunan Co-Innovation Center for Ultilization of Botanical Functional Ingredients, Changsha 410128, China.
Xiang-Yang
XY
Lu
D042682:Angiopoietins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D004195:Disease Models, Animal; D005960:Glucosides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D047493:PPAR alpha / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects*
D042682:Angiopoietins; D005960:Glucosides; D047493:PPAR alpha; C118117:angiopoietin 4; C075528:phillyrin
Angiopoietin-like protein 4; Forsythia suspense; Obese C57BL/6J mice; Peroxisome proliferator-activated receptor beta/delta; Phillyrin