Genotoxic and cytotoxic evaluation of Jatropha dioica Sessé ex Cerv. by the micronucleus test in mouse peripheral blood.
Jatropha dioica Sessé ex Cerv. is a medicinal plant credited with low cytotoxicity in vitro. Thus, the objective of this work was to evaluate the possible genotoxic and cytotoxic effect in vivo of the J. dioica aqueous extract by means of micronucleus assay in mouse peripheral blood. Four different J. dioica aqueous extract dose-units were evaluated (30, 60, 100, and 300 mg/kg). The extract was administered orally to male Balb-C-strain mice every 24 h during 5 days. Blood samples were taken at 0, 24, 48, 72, 96, and 120 h from the mouse's tail and were performed in duplicate extensions. The number of Polychromatic Erythrocytes (PCE), Polychromatic Micronucleus Erythrocytes (PCEMN), and Micronucleus Erythrocytes (MNE) was determined at the different sampling times in the different study groups. Our results showed that the group that received 60 mg/kg of cyclophosphamide (positive control) presented a significant decrease in the PCE (p = 0.044) proportion and a significant increase in MNE (p = 0.032, p = 0.0001). The groups that received the different J. dioica aqueous extract doses did not present either a PCE decrease or an increase in PCEMN and MNE. J. dioica exerts neither a genotoxic nor a cytotoxic effect on mouse peripheral blood at high doses.
Maestría en Ciencias Biomédicas, Universidad Autónoma de Zacatecas, Zacatecas, Mexico.
Diana Isela
DI
Araujo-Espino
Instituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
Ana Lourdes
AL
Zamora-Perez
Laboratorio de Mutagenesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico.
Guillermo Moisés
GM
Zúñiga-González
Doctorado en Farmacología Médica y Molecular, Unidad Académica de Medicina Humana, Universidad Autónoma de Zacatecas, Zacatecas, Mexico.
Rosalinda
R
Gutiérrez-Hernández
Instituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Doctorado en Farmacología, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
Gabriela
G
Morales-Velazquez
Laboratorio de Etnofarmacología, Unidad Académica de Ciencias Químicas, Universidad Autónoma de Zacatecas, Zacatecas, Mexico. Electronic address: blancalazalde@gmail.com.
Blanca Patricia
BP
Lazalde-Ramos
D000818:Animals; D004249:DNA Damage; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D031286:Jatropha / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000633:toxicity*
D010936:Plant Extracts
Cytotoxicity; Erythrocytes; Genotoxicity; Jatropha dioica Sessé ex Cerv.; Mice; Micronuclei; Peripheral blood