Differential expression and characterization of cypermethrin-degrading potential proteins in Bacillus thuringiensis strain, SG4.
A cypermethrin-degrading bacterium (SG4) was isolated from the pesticide-contaminated soil in the agricultural field of the crop research centre of the University, and characterized as Bacillus thuringiensis strain, SG4. The bacterium degraded 78.9 % of cypermethrin (50 ppm) in 15 days when grown in a minimal medium. To understand the functional proteins of cypermethrin degradation in Bacillus thuringiensis strain SG4, a comparative proteomic analysis was performed in the presence/absence of cypermethrin after 5 days of incubation in minimal medium. More than 450 spots corresponding to different proteins were recorded by 2D electrophoresis. We report expression of 223 and 250 unique proteins under normal and induced conditions (cypermethrin stress), respectively. Identified proteins were categorized into different functional groups on the basis of their biological functions, viz., catabolic enzymes, translational and stress proteins, etc. Characterization of cypermethrin-specific proteins in a bacterial strain will help in biodegradation practices in situ.
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, US Nagar, Pantnagar, India. pankajbhatt.bhatt472@gmail.com.
http://orcid.org/0000-0002-0356-5715
Pankaj
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, US Nagar, Pantnagar, India.
Geeta
G
Negi
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, US Nagar, Pantnagar, India.
Saurabh
S
Gangola
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, US Nagar, Pantnagar, India.
Priyanka
P
Khati
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, US Nagar, Pantnagar, India.
Govind
G
Kumar
Department of Chemistry, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, US Nagar, Pantnagar, India.
Anjana
A
Srivastava
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, US Nagar, Pantnagar, India.
Anita
A
Sharma
Bacillus thuringiensis; Biodegradation; Cypermethrin; Proteomics; Two-dimensional gel electrophoresis