Development and validation of a LC-MS/MS method for the determination of isoeugenol in finfish.
A simple, rapid, selective and sensitive LC-MS/MS method was developed and validated for the determination of isoeugenol in finfish using an off-line dansylation derivatization step to enhance signal intensity. The sample was extracted with 10mL acetone using a tissue homogenizer, followed by derivatization with dansyl chloride and analysis by liquid chromatography tandem mass spectrometry. The method detection limit ranged from 0.2 to 0.7ngg-1in six fish species. The limit of quantification was 2.5ngg-1in each species. Recoveries of isoeugenol at three different levels (5, 10 and 20ngg-1) over two different days ranged from 91.2% to 108.0%. Precision (%RSDR) values determined at 10ngg-1were 4.3%, 8.0%, 5.3%, 5.1%, 2.6% and 8.0% for tilapia, catfish, trout, salmon, hybrid striped bass and yellow perch, respectively. Studies of linearity in the range 2.5-40ngg-1produced determination coefficients (R2) above 0.997 for all matrices.
U.S. Food and Drug Administration, Southeast Regional Laboratory, 60 8th Street NE, Atlanta, GA 30309, USA. Electronic address: lin.ye@fda.hhs.gov.
Lin
L
Ye
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005054:Eugenol / Q000031:analogs & derivatives* / Q000737:chemistry; D005399:Fishes; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005054:Eugenol; C036643:isoeugenol
Finfish; Isoeugenol; Isoeugenol (PubChem CID: 853433); LC-MS/MS; Validation parameters