Microscale Symmetrical Electroporator Array as a Versatile Molecular Delivery System.
Successful developments of new therapeutic strategies often rely on the ability to deliver exogenous molecules into cytosol. We have developed a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential intracellular delivery of multiple molecules into various cell types at low voltage in a dosage-controlled manner. Micro-patterned planar electrodes permit substantial reduction in operational voltages and seamless integration with an existing microfluidic technology. Equipped with real-time process visualization functionality, the system enables on-chip optimization of electroporation parameters for cells with varying properties. Moreover, the system's dosage control and multi-molecular delivery capabilities facilitate intracellular delivery of various molecules as a single agent or in combination and its utility in biological research has been demonstrated by conducting RNA interference assays. We envision the system to be a powerful tool, aiding a wide range of applications, requiring single-cell level co-administrations of multiple molecules with controlled dosages.
Rowland Institute at Harvard University, 100 Edwin H. Land Blvd., Cambridge, MA 02142, USA.
Mengxing
M
Ouyang
Rowland Institute at Harvard University, 100 Edwin H. Land Blvd., Cambridge, MA 02142, USA.
Winfield
W
Hill
Massachusetts General Hospital, Charlestown, MA 02129, USA.
Jung Hyun
JH
Lee
Rowland Institute at Harvard University, 100 Edwin H. Land Blvd., Cambridge, MA 02142, USA.
Soojung Claire
SC
Hur
D002232:Carbocyanines / Q000378:metabolism; D045744:Cell Line, Tumor; D018274:Electroporation / Q000295:instrumentation*; D004867:Equipment Design; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism; D055616:Microtechnology / Q000295:instrumentation*; D010047:Ovalbumin / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
C569686:Alexa Fluor 647; D002232:Carbocyanines; D035683:MicroRNAs; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; D010047:Ovalbumin