Contribution of MAML1 in esophageal squamous cell carcinoma tumorigenesis.
BACKGROUND
Notch signaling pathway is involved in different cellular and developmental processes including cell proliferation, differentiation and apoptosis. Mastermind like1 (MAML1) is a critical key transcription coactivator of this pathway. In this study, we aimed to examine MAML1 protein expression in esophageal squamous cell carcinoma (ESCC) and reveal its association with clinicopathological variables of the patients.


METHODS
Tumoral and their margin normal tissues from 56 ESCC patients were recruited for protein expression analysis using immunohistochemistry (IHC). Furthermore, MAML1 expression was analyzed in ESCC cell line KYSE-30 using immunocytochemistry.


RESULTS
Overexpression of MAML1 was detected in 59% of tumor samples. It was significantly associated with different indices of poor prognosis including depth of tumor invasion (P=0.026), grade of tumor differentiation (P=0.002), stage of tumor progression (P=0.004) and sex (P=0.027).


CONCLUSIONS
Beside the appearing evidences explaining MAML1 role in different cellular processes and its deviations in different malignancies and also based on its correlation with different clinicopathological variables of ESCC, MAML1 can be proposed as potentially novel molecular marker for ESCC progression and tumorigenesis as well as therapeutic target to inhibit and reverse progression and development of the disease.
Department of Biology, Damghan branch, Islamic Azad University, Damghan, Iran.; Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: mahnaz_biochemistry@yahoo.com.
Mahnaz
M
Hashemi Bidokhti
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abbaszadeganmr@mums.ac.ir.
Mohammad Reza
MR
Abbaszadegan
Department of Pathology, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: sharifin@mums.ac.ir.
Noorieh
N
Sharifi
Department of Biology, Damghan branch, Islamic Azad University, Damghan, Iran.; Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: sabbasisani@yahoo.com.
Soodabeh
S
Abbasi Sani
Department of Biology, Damghan branch, Islamic Azad University, Damghan, Iran.. Electronic address: forghanifard@gmail.com.
Mohammad Mahdi
MM
Forghanifard
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D049109:Cell Proliferation / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics*
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C430872:MAML1 protein, human; D014157:Transcription Factors
Esophageal squamous cell carcinoma (ESCC); Immunocytochemistry; Immunohistochemistry (IHC); Mastermind like1 (MAML1); Notch intracellular domain (NICD); Notch signaling pathway