Neuroprotective Lignans from the Fruits of Schisandra bicolor var. tuberculata.
Nine new lignans (1-9) and ten known analogues (10-19) were isolated from the fruits of Schisandra bicolor var. tuberculata. The structures of compounds 1-9 were established on the basis of spectroscopic data analysis. The absolute configuration of compound 1 was determined by single-crystal X-ray diffraction analysis with Cu Kα irradiation techniques, and the absolute configurations of compounds 2-9 were deduced by comparing their experimental ECD spectra and optical rotations with those of compound 1 or similar compounds. All isolates were evaluated for their neuroprotective activities against CoCl2, H2O2, and Aβ25-35-induced SH-SY5Y cell injury, and were found to exhibit different degrees of neuroprotective effects. At a low concentration of 3.2 nM, compounds 3, 8, 9, and 14-19 in CoCl2-induced, compounds 7, 8, 13, 17, and 18 in H2O2-induced, and compounds 2, 6, 7, 9, 10, and 12-19 in Aβ25-35-induced SH-SY5Y cell injury models, showed statistically significant neuroprotective activities, when compared with each negative control group.
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
Ye
Y
Liu
Department of Pharmacy, Tongji Hospital Affiliated Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
Heng-Yi
HY
Yu
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
Yan-Mei
YM
Wang
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
Tian
T
Tian
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
Wen-Ming
WM
Wu
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
Ming
M
Zhou
College of Chemistry, Central China Normal University , Wuhan 430079, People's Republic of China.
Xiang-Gao
XG
Meng
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
Han-Li
0000-0003-0882-1009
HL
Ruan
D016229:Amyloid beta-Peptides / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008968:Molecular Conformation; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010446:Peptide Fragments / Q000187:drug effects; D032141:Schisandra / Q000737:chemistry*; D014961:X-Ray Diffraction
D016229:Amyloid beta-Peptides; D017705:Lignans; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D006861:Hydrogen Peroxide