[Expression and effect of S100A9 in the periodontium of diabetic rats].
OBJECTIVE
The study seeks to investigate the expression of S100A9 and its potential role in periodontal diseases induced by diabetes.


METHODS
A diabetic SD rat model was established through intraperitoneal injection of streptozotocin (STZ). Hematoxylin-eosin (HE) staining was performed to study the structure of the periodontium of diabetic rats. Using immunohistochemical staining, the distribution of S100A9 expression was detected in the periodontium of diabetic rats. Ex-pressions of Toll-like receptor 4 (TLR4) (ligands of S100A9) and p-P65/nuclear factor κB (NF-κB) were also measured.


RESULTS
The trabecular structure of alveolar bone was sparser, and lamina dura was disappeared in diabetic rats. Obviously higher expressions of S100A9 were observed in the periodontal ligament, alveolar bone, and gingival epithelial of diabetic rats than in the control rats. TLR4 expressions in the periodontal ligament, alveolar bone and gingival epithelial of the diabetic rats were also higher as compared to the control rats. p-P65 expression was not detected in the control rats, but was detected in the periodontal ligament and alveolar bone of the diabetic rats.


CONCLUSIONS
Periodontium lesions in diabetes mellitus may be induced by the activation of TLR4 and NF-κB signaling pathway meditated by S100A9.  .
College of Life Science, Sichuan University, Chengdu 610065, China;State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China.
Sun
S
Wenhua
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China.
Chen
C
Guoqing
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China.
Tian
T
Weidong
D000818:Animals; D003921:Diabetes Mellitus, Experimental; D005881:Gingiva; D016328:NF-kappa B; D010519:Periodontium; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013311:Streptozocin; D051197:Toll-Like Receptor 4
D016328:NF-kappa B; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D013311:Streptozocin
S100A9; Toll-like receptor 4; diabetes; nuclear factor κB; periodontal lesion