Planarian homolog of puromycin-sensitive aminopeptidase DjPsa is required for brain regeneration.
Puromycin-sensitive aminopeptidase (PSA) belongs to the M1 zinc metallopeptidase family. PSA is the most abundant aminopeptidase in the brain and plays a role in the metabolism of neuropeptides including those involved in neurodegeneration. A cDNA DjPsa was identified from the planarian Dugesia japonica cDNA library. It contains a 639-bp open reading frame corresponding to a deduced protein of 212 amino acids. Whole mount in situ hybridization revealed that DjPsa is expressed in the brain and ventral nerve cords of intact and regenerating animals and demonstrates a tissue and stage-specific expression pattern of DjPsa in developing embryos and larvae. Knocking down DjPsa gene expression with RNA interference during planarian regeneration inhibits the brain reformation completely. The results suggest that DjPsa is required for planarian brain regeneration.
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo, 255049, People's Republic of China.
Suge
S
Wu
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo, 255049, People's Republic of China.
Bin
B
Liu
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo, 255049, People's Republic of China.|School of Life Sciences, Shandong University of Technology, 266 Xincun Western Road, Zibo, 255049, People's Republic of China.
Zuoqing
Z
Yuan
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo, 255049, People's Republic of China.|School of Life Sciences, Shandong University of Technology, 266 Xincun Western Road, Zibo, 255049, People's Republic of China.
Xiufang
X
Zhang
School of Life Sciences, Shandong University of Technology, 266 Xincun Western Road, Zibo, 255049, People's Republic of China.
Hong
H
Liu
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo, 255049, People's Republic of China.|School of Life Sciences, Shandong University of Technology, 266 Xincun Western Road, Zibo, 255049, People's Republic of China.
Qiuxiang
Q
Pang
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo, 255049, People's Republic of China. zhaobosheng@sdut.edu.cn.|School of Life Sciences, Shandong University of Technology, 266 Xincun Western Road, Zibo, 255049, People's Republic of China. zhaobosheng@sdut.edu.cn.
Bosheng
B
Zhao
D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D001921:Brain / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D004625:Embryo, Nonmammalian; D015723:Gene Library; D007814:Larva; D010932:Planarians / Q000196:embryology / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D034622:RNA Interference / Q000502:physiology; D034741:RNA, Small Interfering / Q000494:pharmacology; D012038:Regeneration / Q000235:genetics*
D034741:RNA, Small Interfering; D000626:Aminopeptidases; C025579:enkephalin degrading enzyme
Brain; DjPsa; Embryo; Planarian; Regeneration