Chloroplast behaviour and interactions with other organelles inArabidopsis thalianapavement cells.
Chloroplasts are a characteristic feature of green plants. Mesophyll cells possess the majority of chloroplasts and it is widely believed that, with the exception of guard cells, the epidermal layer in most higher plants does not contain chloroplasts. However, recent observations onArabidopsis thalianahave shown a population of chloroplasts in pavement cells that are smaller than mesophyll chloroplasts and have a high stroma to grana ratio. Here, using stable transgenic lines expressing fluorescent proteins targeted to the plastid stroma, plasma membrane, endoplasmic reticulum, tonoplast, nucleus, mitochondria, peroxisomes, F-actin and microtubules, we characterize the spatiotemporal relationships between the pavement cell chloroplasts (PCCs) and their subcellular environment. Observations on the PCCs suggest a source-sink relationship between the epidermal and the mesophyll layers, and experiments with theArabidopsismutantsglabra2(gl2) andimmutans(im), which show altered epidermal plastid development, underscored their developmental plasticity. Our findings lay down the foundation for further investigations aimed at understanding the precise role and contributions of PCCs in plant interactions with the environment.
Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, Ontario N1G2W1, Canada.
Kiah A
KA
Barton
Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, Ontario N1G2W1, Canada.
Michael R
MR
Wozny
Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, Ontario N1G2W1, Canada.
Neeta
N
Mathur
Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, Ontario N1G2W1, Canada.
Erica-Ashley
EA
Jaipargas
Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, Ontario N1G2W1, Canada jmathur@uoguelph.ca.
Jaideep
0000-0002-8806-1486
J
Mathur
D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism* / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics; D015388:Organelles / Q000378:metabolism*; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism; D030821:Plants, Genetically Modified; D059008:Time-Lapse Imaging; D064201:Trichomes / Q000378:metabolism / Q000648:ultrastructure
D002734:Chlorophyll
Chloroplasts; Epidermis; Organelle interactions; Pavement cells; Stromules