Early-postnatal iron deficiency impacts plasticity in the dorsal and ventral hippocampus in piglets.
In this study, we investigated whether alterations in plasticity markers such as brain-derived neurotrophic factor (BDNF), p75 neurotrophin receptor (p75NTR) and tyrosine receptor kinase B (TrkB) are underlying iron deficiency (ID)-induced cognitive impairments in iron depleted piglets. Newborn piglets were either fed an iron-depleted diet (21mg Fe/kg) or an iron-sufficient diet (88mg Fe/kg) for four weeks. Subsequently, eight weeks after iron repletion (190-240mg Fe/kg) we found a significant decrease in mature BDNF (14kDa) and proBDNF (18kDa and 24kDa) protein levels in the ventral hippocampus, whereas we found increases in the dorsal hippocampus. The phosphorylation of cAMP response element binding protein (CREB) follows the mature BDNF protein level pattern. No effects were found on BDNF and CREB protein levels in the prefrontal cortex. The protein levels of the high affinity BDNF receptor, TrkB, was significantly decreased in both dorsal and ventral hippocampus of ID piglets, whereas it was increased in the prefrontal cortex. Together, our data suggest a disrupted hippocampal plasticity upon postnatal ID.
Dept. of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.
Ellis
E
Nelissen
Dept. of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.
Jochen
J
De Vry
Behavior & Welfare Group (formerly Emotion & Cognition Group), Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Alexandra
A
Antonides
Dept. of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.
Dean
D
Paes
Dept. of Neuroimmunology and Biochemistry, BIOMED, Hasselt University, Hasselt, Belgium.
Melissa
M
Schepers
Behavior & Welfare Group (formerly Emotion & Cognition Group), Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Franz Josef
FJ
van der Staay
Dept. of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.
Jos
J
Prickaerts
Dept. of Neuroimmunology and Biochemistry, BIOMED, Hasselt University, Hasselt, Belgium. Electronic address: tim.vanmierlo@uhasselt.be.
Tim
T
Vanmierlo
D018798:Anemia, Iron-Deficiency / Q000150:complications* / Q000209:etiology; D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D050882:CREB-Binding Protein / Q000378:metabolism; D003072:Cognition Disorders / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D000076122:Disks Large Homolog 4 Protein / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006624:Hippocampus / Q000254:growth & development* / Q000378:metabolism*; D008297:Male; D009473:Neuronal Plasticity / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013552:Swine; D016708:Synaptophysin / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D000076122:Disks Large Homolog 4 Protein; D016708:Synaptophysin; D050882:CREB-Binding Protein; D010751:Phosphopyruvate Hydratase
BDNF; Iron deficiency; Neuronal plasticity; Pig