Organism and Microbiome Analysis: Techniques and Implications for Chronic Rhinosinusitis.
Modern advances in DNA sequencing have allowed for the development of culture-independent techniques with application to infectious and inflammatory conditions, such as rhinosinusitis. Although paradigm-changing discoveries have resulted from molecular microbiologic methods for a number of diseases, insights provided into the role of bacteria in chronic rhinosinusitis have yet to be fully understood to the point of impacting clinical diagnosis and management. As culture-independent techniques continue to evolve and become more refined, it is likely that a better understanding will emerge of how the microbiome influences chronic rhinosinusitis pathogenesis and response to therapy.
Department of Otolaryngology-Head and Neck Surgery, University of Southwestern Medical Center, 5303 Harry Hines Blvd, Dallas, TX 75390-9035, USA.
Ashleigh A
AA
Halderman
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins School of Medicine, 6th Floor, 601 North Caroline Street, Baltimore, MD 21287-0910, USA. Electronic address: alane3@jhmi.edu.
Andrew P
AP
Lane
D001419:Bacteria / Q000145:classification / Q000235:genetics; D002908:Chronic Disease; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D054892:Metagenome / Q000235:genetics; D064307:Microbiota; D010256:Paranasal Sinuses / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012220:Rhinitis / Q000382:microbiology*; D012852:Sinusitis / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S
Chronic rhinosinusitis; Metagenome; Microbiota; RNA, Ribosomal, 16S; Sequence analysis, DNA