Fabrication of sensitive enzymatic biosensor based on multi-layered reduced graphene oxide added PtAu nanoparticles-modified hybrid electrode.
A highly sensitive amperometric glucose sensor was developed by immobilization of glucose oxidase (GOx) onto multi-layer reduced graphene oxide (MRGO) sheets decorated with platinum and gold flower-like nanoparticles (PtAuNPs) modified Au substrate electrode. The fabricated MRGO/PtAuNPs modified hybrid electrode demonstrated high electrocatalytic activities toward oxidation of H2O2, to which it had a wide linear response that ranged from 0.5 to 8 mM (R2 = 0.997), and high sensitivity of 506.25 μA/mMcm2. Furthermore, glucose oxidase-chitosan composite and cationic polydiallyldimethylammonium chloride (PDDA) were assembled by a casting method on the surface of MRGO/PtAuNPs modified electrode. This as-fabricated hybrid biosensor electrode exhibited high electrocatalytic activity for the detection of glucose in PBS. It demonstrated good analytical properties in terms of a low detection limit of 1 μM (signal-to-noise ratio of 3), short response time (3 s), high sensitivity (17.85 μA/mMcm2), and a wide linear range (0.01-8 mM) for glucose sensing. These results reveal that the newly developed sensing electrode offers great promise for new type enzymatic biosensor applications.
Department of Electronic Engineering, Kwangwoon University, Nowon Gu, Seoul, Korea.
Md Faruk
MF
Hossain
Department of Electronic Engineering, Kwangwoon University, Nowon Gu, Seoul, Korea.
Jae Y
JY
Park
D015374:Biosensing Techniques / Q000379:methods; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D059629:Signal-To-Noise Ratio
D004800:Enzymes, Immobilized; D005663:Furans; D010087:Oxides; C090072:graphenone; D010984:Platinum; D006046:Gold; D006108:Graphite; D048271:Chitosan; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose