DNAzyme-aptamer or aptamer-DNAzyme paradigm: Biochemical approach for aflatoxin analysis.
DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to differentiate between these two conjugation patterns. Results showed that DNAzyme-aptamer has more catalytic activity and efficiency than aptamer-DNAzyme. Thereby, DNAzyme-aptamer with its superior efficiency can be used for design and development of more sensitive aflatoxin B1 DNA based biosensors.
Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Marzieh
M
Jafari
Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.|Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Mohsen
M
Rezaei
Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Heibatullah
H
Kalantari
Protein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Maryam
M
Tabarzad
Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Bahram
B
Daraei
D016604:Aflatoxin B1 / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001560:Benzidines / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002863:Chromogenic Compounds / Q000032:analysis; D021881:DNA, Catalytic / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry
D052157:Aptamers, Nucleotide; D001560:Benzidines; D002863:Chromogenic Compounds; D021881:DNA, Catalytic; C021758:3,3',5,5'-tetramethylbenzidine; D016604:Aflatoxin B1; D006861:Hydrogen Peroxide; D009195:Peroxidase
DNAzyme; aptamer; peroxidase activity