Dietary Myrtle (Myrtus communis L.) improved non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (Oncorhynchus mykiss) fingerlings.
The present study examined the effects of dietary Myrtle (Myrtus communis L.) on non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (Oncorhynchus mykiss) fingerlings. Three hundred and sixty fingerlings (6.50 ± 0.55 g (were distributed in twelve cages (65 × 65 × 65 cm) with a metal framework. The study included four treatments repeated in triplicates. The treatments were feeding trouts with experimental diets containing different levels (0, 0.5, 1 and 1.5%) of Myrtle powder. The fingerlings were fed on experimental diet for sixty days and then skin mucus non-specific immune parameters as well as bactericidal activity were measured. At the end of the trial, the highest skin mucus soluble protein level was observed in group fed with 1.5% Myrtle (P < 0.05). The alkaline phosphatase (ALP) activity was significantly increased in fish groups fed 1 and 1.5% Myrtle compared with the control group (P < 0.05). However, evaluation of skin mucus lysozyme activity showed no significant difference between treatments and control group (P > 0.05). Also, no antibacterial activity was detected against Escherichia coli, Staphylococcus aureus and Salmonella enterica in all treatments and control group. Whereas skin mucus of rainbow trout showed antimicrobial activity against fish pathogens (Aeromonas hydrophila and Yersinia ruckeri) in 1 and 1.5% Myrtle treatments. These results indicated beneficial effects of dietary Myrtle on mucosal immune parameters of fingerling rainbow trout.
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: mansouritaee@gmail.com.
Hadis
H
Mansouri Taee
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: ahajimoradloo@yahoo.com.
Abdolmajid
A
Hajimoradloo
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Seyed Hossein
SH
Hoseinifar
Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khoram Abad, Iran.
Hassan
H
Ahmadvand
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D007113:Immunity, Innate; D009093:Mucus / Q000276:immunology*; D031651:Myrtus / Q000737:chemistry*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D012867:Skin / Q000276:immunology
D000900:Anti-Bacterial Agents; D010936:Plant Extracts
Bactericidal activity; Mucosal immunity; Myrtle; Non-specific immunity; Rainbow trout; Skin