Analysis ofG3BP1andVEZTExpression in Gastric Cancer and Their Possible Correlation with Tumor Clinicopathological Factors.
OBJECTIVE
This study aimed to analyzeG3BP1andVEZTexpression profiles in patients with gastric cancer, and examine the possible relationship between the expressions of each gene and clinicopathological factors.


METHODS
Expression of these genes in formalin-fixed paraffin embedded (FFPE) tissues, collected from 40 patients with gastric cancer and 40 healthy controls, was analyzed. Differences in gene expression among patient and normal samples were identified using the GraphPad Prism 5 software. For the analysis of real-time polymerase chain reaction products, GelQuantNET software was used.


RESULTS
Our findings demonstrated that bothVEZTandG3BP1mRNA expression levels were downregulated in gastric cancer samples compared with those in the normal controls. No significant relationship was found between the expression of these genes and gender (P-value, 0.4835 vs. 0.6350), but there were significant changes associated with age (P-value, 0.0004 vs. 0.0001) and stage of disease (P-value, 0.0019 vs. 0.0001). In addition, there was a direct relationship betweenVEZTgene expression and metastasis (P-value, 0.0462), in contrast toG3BP1that did not demonstrate any significant correlation (P-value, 0.1833).


CONCLUSIONS
The results suggest that expression profiling ofVEZTandG3BP1can be used for diagnosis of gastric cancer, and specifically,VEZTgene could be considered as a biomarker for the detection of gastric cancer progression.
Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran .
Mohammadreza
M
Beheshtizadeh
Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran .
Elham
E
Moslemi
G3BP1; Gene expression; Stomach neoplasms; VEZT