Recent Advances in Development of Genetically Encoded Fluorescent Sensors.
Genetically encoded fluorescent sensors are essential tools in modern biological research, and recent advances in fluorescent proteins (FPs) have expanded the scope of sensor design and implementation. In this review we compare different sensor platforms, including Förster resonance energy transfer (FRET) sensors, fluorescence-modulated single FP-based sensors, translocation sensors, complementation sensors, and dimerization-based sensors. We discuss elements of sensor design and engineering for each platform, including the incorporation of new types of FPs and sensor screening techniques. Finally, we summarize the wide range of sensors in the literature, exploring creative new sensor architectures suitable for different applications.
University of Colorado Boulder, Boulder, CO, United States.
Lynn
L
Sanford
University of Colorado Boulder, Boulder, CO, United States. Electronic address: amy.palmer@colorado.edu.
Amy
A
Palmer
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics
D005456:Fluorescent Dyes; D008164:Luminescent Proteins
Complementation sensor; Dimerization sensor; FRET sensor; Fluorescent protein; Fluorescent sensor; Genetically encoded sensor; Sensor engineering; Single FP sensor; Translocation sensor