Ultrasensitive detection of clenbuterol by a covalent imprinted polymer as a biomimetic antibody.
As an ideal biomimetic antibody, molecularly imprinted polymer (MIP) has shown great promise in immunoassays. Here, we developed a covalent imprinting method to prepare MIP artificial antibody towards clenbuterol on the well surface of a microtiter plate. Template molecule (clenbuterol) was splited by hydrolysis with functional monomer and removed by methanol/acetic acid solution, and then three-dimensional cavities were fabricated in the MIP, which can rebind template molecule via hydrogen bond interaction. Using this artificial antibody, we developed a novel biomimetic enzyme-linked immunosorbent assay (ELISA) with excellent sensitivity and specificity. The linear range for clenbuterol was 10-5to 1000μgL-1, with a detection limit of 10-7μgL-1. Cross reactivity of this MIP artificial antibody to other four structural analogs was less than 0.93%. This method was applied to determine clenbuterol in real samples with satisfactory result, suggesting a promising application of the biomimetic ELISA in food and environmental analysis.
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China; Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver V6T 1Z4, Canada. Electronic address: tangyiwei81@163.com.
Yiwei
Y
Tang
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China.
Jingwen
J
Gao
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China.
Xiuying
X
Liu
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China.
Xue
X
Gao
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China. Electronic address: matao-09@163.com.
Tao
T
Ma
Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver V6T 1Z4, Canada. Electronic address: xiaonan.lu@ubc.ca.
Xiaonan
X
Lu
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China. Electronic address: lijr6491@163.com.
Jianrong
J
Li
D000318:Adrenergic beta-Agonists / Q000032:analysis / Q000737:chemistry*; D000906:Antibodies; D032701:Biomimetics / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis* / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*
D000318:Adrenergic beta-Agonists; D000906:Antibodies; D011108:Polymers; D002976:Clenbuterol
Artificial antibody; Biomimetic ELISA; Clenbuterol; Molecularly imprinted polymer