[Intestinal coccidiosis in children admitted to a hospital in Peru and comparison of two methods for detecting Cryptosporidium spp].
A cross-sectional study was done between October 2014 and June 2015 to estimate the frequency of infection due to intestinal coccidiosis in children admitted to a hospital in Peru, and compare the modified acid-fast staining procedure with the enzyme-linked immunoassay (ELISA) method for the detection of Cryptosporidium spp. Coccidia were detected using the modified acid-fast staining procedure and the Cryptosporidium ELISA method (kit r-Biopharm) on seriated stool samples. Out of a total of 325 children, 5.5% had some type of intestinal coccidiosis: 3.7% involved Cryptosporidium spp. (using both techniques) and 1.8% involved Cyclospora cayetanensis (using the modified acid-fast staining procedure). The modified acid-fast staining procedure and ELISA method revealed a 0.955 consistency in the detection of Cryptosporidium spp. In conclusion, intestinal coccidiosis is frequent in children based on the population studied; similarly, both techniques can be used to detect Cryptosporidium spp., but the modified acid-fast staining procedure has an advantage over the other because it is cost-effective and can detect other coccidia in everyday practice.
Laboratorio de Parasitología, Metaxénicas y Zoonosis, Hospital Regional Lambayeque. Lambayeque, Perú.
Heber
H
Silva-Díaz
Facultad de Ciencias de la Salud, Universidad de Chiclayo. Lambayeque, Perú.
Hamer
H
Campos-Flores
Facultad de Ciencias de la Salud, Universidad de Chiclayo. Lambayeque, Perú.
Jean Pier
JP
Llagas-Linares
Laboratorio de Parasitología, Metaxénicas y Zoonosis, Hospital Regional Lambayeque. Lambayeque, Perú.
Dunalia
D
LLatas-Cancino
D002648:Child; D002675:Child, Preschool; D003048:Coccidiosis / Q000175:diagnosis* / Q000453:epidemiology; D003430:Cross-Sectional Studies; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces; D005260:Female; D006801:Humans; D008297:Male; D010568:Peru