Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality.
Hyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects.



This prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/mL) used for denudation of sibling-oocytes for 22 women undergoing treatment for assisted reproduction by ICSI. A total of 192 oocytes were injected, being 104 for group I (diluted) and 88 for group II (normal). We analyzed fertilization rate, cleavage, embryo quality at 48 and 72 hours and number of transferred embryos in each group.



The diluted enzyme group showed better results in fertilization rates (92.3% vs. 80.6%), mean cleavage (4.18 ± 2.57 vs. 3.09 ± 1.90), in 48-hour embryos A and A + B (60.9% vs. 44.1% and 90.2% vs. 82.3%) and at 72 hours (45.6% vs. 36.8% and 77.1% vs 66.2%), and number of embryos selected for transfer (61.8% vs. 38.1%). The overall pregnancy rate was 59.1%.



This study demonstrates that the use of 8 IU/mL of hyaluronidase, according to the following protocol, is beneficial and can be successfully used for oocyte denudation, and it is also economically advantageous to the laboratory.
Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.|Universidade Anhembi Morumbi, São Paulo, SP, Brazil.
Bernardo R L de
BR
Moura
Universidade Anhembi Morumbi, São Paulo, SP, Brazil.
Mariana C A
MC
Gurgel
Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
Suelen Pp
SP
Machado
Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
Priscila A
PA
Marques
Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
Juliana R
JR
Rolim
Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
Marcelo C de
MC
Lima
Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
Lister L
LL
Salgueiro
D005260:Female; D005306:Fertilization; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology*; D054315:Oocyte Retrieval; D009865:Oocytes / Q000187:drug effects*; D011247:Pregnancy; D018873:Pregnancy Rate; D011897:Random Allocation; D020554:Sperm Injections, Intracytoplasmic
D006821:Hyaluronoglucosaminidase
Hyaluronidase; ICSI; cumulus- oocyte complex; oocyte denudation