AGR2 promotes the proliferation, migration and regulates epithelial-mesenchymal transition in salivary adenoid cystic carcinoma.
Salivary adenoid cystic carcinoma (AdCC) is a common head and neck cancer with the propensity for local spread and distant metastasis. In our previous study, elevated expression of Anterior gradient 2 (AGR2) was detected in head and neck squamous cell carcinoma (HNSCC), associated with epithelial-mesenchymal transition (EMT) and cancer stemness. However, to date, the expression and function of AGR2 in AdCC has yet to be elucidated. In the present study, human AdCC tissue microarrays including 18 cases of normal salivary gland (NSG), 12 cases of pleomorphic adenoma (PMA) and 72 cases of AdCC were employed for immunohistochemical staining analysis. Results indicated that AGR2, which was remarkably correlated with Ki-67, transforming growth factor beta-1 (TGF-β1) and CD147, was significantly elevated in human salivary AdCC tissues. Knockdown of AGR2 significantly repressed the proliferation and migration of human SACC-83 and SACC-LM cell lines. Additionally, AGR2 silencing obviously reversed the EMT phenomena induced by TGF-β1. Taken together, our present study revealed the potential pro-metastasis role of AGR2 in AdCC, indicating that AGR2 might be a novel therapeutic target of AdCC with distant metastasis.
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education.
Si-Rui
SR
Ma
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education.
Liang
L
Mao
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education.
Wei-Wei
WW
Deng
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education.
Yi-Cun
YC
Li
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education.
Lin-Lin
LL
Bu
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education.
Guang-Tao
GT
Yu
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral BiomedicineMinistry of Education; Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan UniversityWuhan, China.
Wen-Feng
WF
Zhang
The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral BiomedicineMinistry of Education; Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan UniversityWuhan, China.
Zhi-Jun
ZJ
Sun
Adenoid cystic carcinoma; Anterior gradient 2; epithelial-mesenchymal transition; migration; proliferation