Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke.
Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice and these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it is essential to understand the traits of iSCs in the poststroke human brain for possible applications in stem cell-based therapies for stroke patients. In this study, we report for the first time that iSCs can be isolated from the poststroke human brain. Putative iSCs were derived from poststroke brain tissue obtained from elderly stroke patients requiring decompressive craniectomy and partial lobectomy for diffuse cerebral infarction. Immunohistochemistry showed that these iSCs were localized near blood vessels within poststroke areas containing apoptotic/necrotic neurons and expressed both the stem cell marker nestin and several pericytic markers. Isolated iSCs expressed these same markers and demonstrated high proliferative potential without loss of stemness. Furthermore, isolated iSCs expressed other stem cell markers, such as Sox2, c-myc, and Klf4, and differentiated into multiple cells in vitro, including neurons. These results show that iSCs, which are likely brain pericyte derivatives, are present within the poststroke human brain. This study suggests that iSCs can contribute to neural repair in patients with stroke.
1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan .
Kotaro
K
Tatebayashi
1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan .|2 Institute for Advanced Medical Sciences , Hyogo College of Medicine, Nishinomiya, Japan .
Yasue
Y
Tanaka
2 Institute for Advanced Medical Sciences , Hyogo College of Medicine, Nishinomiya, Japan .
Akiko
A
Nakano-Doi
2 Institute for Advanced Medical Sciences , Hyogo College of Medicine, Nishinomiya, Japan .
Rika
R
Sakuma
2 Institute for Advanced Medical Sciences , Hyogo College of Medicine, Nishinomiya, Japan .
Saeko
S
Kamachi
1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan .
Manabu
M
Shirakawa
1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan .
Kazutaka
K
Uchida
1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan .
Hiroto
H
Kageyama
1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan .
Toshinori
T
Takagi
1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan .
Shinichi
S
Yoshimura
2 Institute for Advanced Medical Sciences , Hyogo College of Medicine, Nishinomiya, Japan .
Tomohiro
T
Matsuyama
2 Institute for Advanced Medical Sciences , Hyogo College of Medicine, Nishinomiya, Japan .
Takayuki
T
Nakagomi
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001921:Brain / Q000473:pathology* / Q000601:surgery; D002545:Brain Ischemia / Q000473:pathology; D002469:Cell Separation; D005260:Female; D006801:Humans; D000090062:Kruppel-Like Factor 4; D008297:Male; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology*; D020286:Pericytes / Q000473:pathology; D020521:Stroke / Q000473:pathology* / Q000601:surgery
human; ischemia; multipotent stem cells; pericyte; stroke