Structural Changes Induced in Grapevine (Vitis vinifera L.) DNA by Femtosecond IR Laser Pulses: A Surface-Enhanced Raman Spectroscopic Study.
In this work, surface-enhanced Raman spectra of ten genomic DNAs extracted from leaf tissues of different grapevine (Vitis viniferaL.) varieties, respectively, are analyzed in the wavenumber range 300-1800 cm-1. Furthermore, structural changes induced in grapevine genomic nucleic acids upon femtosecond (170 fs) infrared (IR) laser pulse irradiation (λ = 1100 nm) are discussed in detail for seven genomic DNAs, respectively. Surface-enhanced Raman spectroscopy (SERS) signatures, vibrational band assignments and structural characterization of genomic DNAs are reported for each case. As a general observation, the wavenumber range between 1500 and 1660 cm-1of the spectra seems to be modified upon laser treatment. This finding could reflect changes in the base-stacking interactions in DNA. Spectral shifts are mainly attributed to purines (dA, dG) and deoxyribose. Pyrimidine residues seem to be less affected by IR femtosecond laser pulse irradiation. Furthermore, changes in the conformational properties of nucleic acid segments are observed after laser treatment. We have found that DNA isolated from Feteasca Neagra grapevine leaf tissues is the most structurally-responsive system to the femtosecond IR laser irradiation process. In addition, using unbiased computational resources by means of principal component analysis (PCA), eight different grapevine varieties were discriminated.
National Institute for Research & Development of Isotopic and Molecular Technologies, Donat 67-103, 400293 Cluj-Napoca, Romania. nicoleta.dina@itim-cj.ro.
Nicoleta E
NE
Dina
National Institute for Research & Development of Isotopic and Molecular Technologies, Donat 67-103, 400293 Cluj-Napoca, Romania. cmuntean@itim-cj.ro.
Cristina M
CM
Muntean
Babeş-Bolyai University, Faculty of Physics, Kogălniceanu 1, 400084 Cluj-Napoca, Romania. nicolae.leopold@phys.ubbcluj.ro.
Nicolae
N
Leopold
National Institute for Research & Development of Isotopic and Molecular Technologies, Donat 67-103, 400293 Cluj-Napoca, Romania. alexandra.falamas@itim-cj.ro.
Alexandra
A
Fălămaș
National Institute of Research and Development for Biological Sciences, branch Institute of Biological Research, Republicii Street 48, 400015 Cluj-Napoca, Romania. adela.halmagyi@icbcluj.ro.
Adela
A
Halmagyi
National Institute of Research and Development for Biological Sciences, branch Institute of Biological Research, Republicii Street 48, 400015 Cluj-Napoca, Romania. ana.coste@icbcluj.ro.
Ana
A
Coste
femtosecond infrared (IR) laser pulses; genomic DNA; principal component analysis discrimination; silver colloid; surface-enhanced Raman spectroscopy