Changes in expression of the CD200 tolerance-signaling molecule and its receptor (CD200R) by villus trophoblasts during first trimester missed abortion and in chronic histiocytic intervillositis.
Expression of CD200 at the feto-maternal interface is associated with successful murine and human pregnancy. CD200 binding to CD200 receptors on lymphomyeloid cells suppresses inflammation and induces Tregs. CD200 receptors are also expressed on mouse and human placental trophoblast cells. What is the expression of CD200 and CD200R in human missed abortions which have preserved Treg levels and in chronic histiocytic intervillositis (CHI) where maternal inflammatory cells cause IUGR?



Immunohistiochemistry for CD200, CD200R, and Ki67 using human placental sections from missed abortions, term placenta, and CHI. PCR testing was done for trisomy in missed abortion.



CD200 and CD200R were expressed by human villus trophoblasts from 2 weeks post-implantation to term. Cytotrophoblast proliferation (Ki-67+count) decreased at term. In first trimester missed abortion cases, CD200>CD200R villus trophoblasts accompanied missed abortion of non-trisomic male fetuses. CD200 and Ki67+trophoblast proliferation was preserved in CHI with maternal inflammatory cell infiltration but CD200R was greatly decreased.



Residual CD200 activity may prevent completion of abortions via induction of Treg cells. In CHI, infiltrating maternal effector T cells may block Treg induction. An autocrine role for CD200-CD200R interaction versus inhibition of soluble CD200 by soluble CD200R is discussed.
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.|Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
David A
0000-0002-8032-1028
DA
Clark
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
Jennifer M
JM
Dmetrichuk
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.|Genetics Services, Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, Ontario, Canada.
Elizabeth
E
McCready
Department of Oncology, McMaster University, Hamilton, Ontario, Canada.
Sukhbinder
S
Dhesy-Thind
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
Jorge L
JL
Arredondo
D000030:Abortion, Missed / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002824:Chorionic Villi / Q000276:immunology*; D005260:Female; D006644:Histiocytes / Q000276:immunology; D006801:Humans; D019394:Ki-67 Antigen / Q000276:immunology; D064446:Orexin Receptors; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000276:immunology*; D015398:Signal Transduction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014327:Trophoblasts / Q000276:immunology
D015703:Antigens, CD; D000954:Antigens, Surface; C477337:CD200R1 protein, human; D019394:Ki-67 Antigen; D064446:Orexin Receptors; D011956:Receptors, Cell Surface; C042739:antigens, CD200
CD200; CD200 receptor; chronic histiocytic intervillositis; missed abortion; tolerance; villus trophoblast