Therapeutic effect of green tea extract on alcohol induced hepatic mitochondrial DNA damage in albino wistar rats.
The present study principally sought to investigate the effect of green tea extract (GTE) supplementation on hepatic mitochondrial DNA (mtDNA) damage in alcohol receiving rats. MtDNA was isolated from hepatic tissues of albino wistar rats after alcohol treatment with and without GTE supplementation. Entire displacement loop (D-loop) of mtDNA was screened by PCR-Sanger's sequencing method. In addition, mtDNA deletions and antioxidant activity were measured in hepatic tissue of all rats. Results showed increased frequency of D-loop mutations in alcoholic rats (ALC). DNAmfoldanalysis predicted higher free energy for15507Cand16116Calleles compared to their corresponding wild alleles which represents less stable secondary structures with negative impact on overall mtDNA function. Interestingly, D-loop mutations observed in ALC rats were successfully restored on GTE supplementation. MtDNA deletions were observed in ALC rats, but intact native mtDNA was found in ALC + GTE group suggesting alcohol induced oxidative damage of mtDNA and ameliorative effect of GTE. Furthermore, markedly decreased activities of glutathione peroxidise, superoxide dismutase, catalase and glutathione content were identified in ALC rats; however, GTE supplementation significantly (P< 0.05) restored these levels close to normal. In conclusion, green tea could be used as an effective nutraceutical against alcohol induced mitochondrial DNA damage.
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515 003, India.
Hymavathi
H
Reddyvari
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515 003, India.
Suresh
S
Govatati
Department of Environmental Sciences, Andhra University, Visakhapatnam 530 003, India.
Sumanth Kumar
SK
Matha
Department of Biotechnology, Dr BR Ambedkar University, Srikakulam 532 410, India.
Swapna Vahini
SV
Korla
Department of Biochemistry, Krishna University Dr. MRAR PG Center, Nuzvid 521 201, India.
Sravanthi
S
Malempati
Department of Advanced Research Centre, Narayana Medical College and Hospital, Nellore 524 003, India.
Sreenivasa Rao
SR
Pasupuleti
Department of Biochemistry, Osmania University, Hyderabad 500 007, India.
Manjula
M
Bhanoori
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur 515 003, India.
Varadacharyulu
V
Nallanchakravarthula
Alcohol; Antioxidant; D-loop; Green tea extract; Mitochondrial DNA; ROS