PCR-Independent Method of Transformation-Associated Recombination Reveals the Cosmomycin Biosynthetic Gene Cluster in an Ocean Streptomycete.
The transformation-associated recombination cloning methodology facilitates the genomic capture and heterologous expression of natural product biosynthetic gene clusters (BGCs). We have streamlined this procedure by introduction of synthetic DNA gene blocks for the efficient capture of BGCs. We show the successful capture and expression of the aromatic polyketide antitumor agent cosmomycin from streptomycete bacteria and the discovery of new cosmomycin analogues by mass spectral molecular networking.
Center for Marine Biotechnology & Biomedicine, Scripps Institution of Oceanography, University of California at San Diego , La Jolla, California 92037, United States.|Biomedical Sciences Graduate Program, University of California at San Diego , La Jolla, California 92093, United States.
Charles B
CB
Larson
Center for Marine Biotechnology & Biomedicine, Scripps Institution of Oceanography, University of California at San Diego , La Jolla, California 92037, United States.
Max
M
Crüsemann
Center for Marine Biotechnology & Biomedicine, Scripps Institution of Oceanography, University of California at San Diego , La Jolla, California 92037, United States.|Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego , La Jolla, California 92093, United States.
Bradley S
0000-0002-4652-1253
BS
Moore
D001688:Biological Products / Q000378:metabolism; D023281:Genomics; D015394:Molecular Structure; D005810:Multigene Family; D009792:Oceans and Seas; D061065:Polyketides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
D001688:Biological Products; D061065:Polyketides; C000621480:cosmomycin