GTPase-activating protein Elmod2 is essential for meiotic progression in mouse oocytes.
Meiotic failure in oocytes is the major determinant of human zygote-originated reproductive diseases, the successful accomplishment of meiosis largely relay on the normal functions of many female fertility factors. Elmod2 is a member of the Elmod family with the strongest GAP (GTPase-activating protein) activity; although it was identified as a possible maternal protein, its actual physiologic role in mammalian oocytes has not been elucidated. Herein we reported that among Elmod family proteins, Elmod2 is the most abundant in mouse oocytes, and that inhibition of Elmod2 by specific siRNA caused severe meiotic delay and abnormal chromosomal segregation during anaphase. Elmod2 knockdown also significantly decreased the rate of oocyte maturation (to MII, with first polar body extrusion), and significantly greater numbers of Elmod2-knockdown MII oocytes were aneuploid. Correspondingly, Elmod2 knockdown dramatically decreased fertilization rate. To investigate the mechanism(s) involved, we found that Elmod2 knockdown caused significantly more abnormal mitochondrial aggregation and diminished cellular ATP levels; and we also found that Elmod2 co-localized and interacted with Arl2, a GTPase that is known to maintain mitochondrial dynamics and ATP levels in oocytes. In summary, we found that Elmod2 is the GAP essential to meiosis progression of mouse oocytes, most likely by regulating mitochondrial dynamics.
a State Key Lab of Reproductive Medicine , Nanjing Medical University , Nanjing , Jiangsu , P.R. China.
Chun-Xiang
CX
Zhou
a State Key Lab of Reproductive Medicine , Nanjing Medical University , Nanjing , Jiangsu , P.R. China.
Li-Ya
LY
Shi
b Liuzhou Worker's Hospital , Liuzhou , Guangxi , China.
Rui-Chao
RC
Li
c The Second Affiliated Hospital , Nanjing Medical University , Nanjing , Jiangsu , China.
Ya-Hong
YH
Liu
c The Second Affiliated Hospital , Nanjing Medical University , Nanjing , Jiangsu , China.
Bo-Qun
BQ
Xu
d Department of Gynecology , Zhejiang Provincial People's Hospital , Hangzhou , Zhejiang , China.
Jin-Wei
JW
Liu
e Wenxi Agriculture Committee , Yuncheng , Shanxi , China.
Bo
B
Yuan
a State Key Lab of Reproductive Medicine , Nanjing Medical University , Nanjing , Jiangsu , P.R. China.
Zhi-Xia
ZX
Yang
c The Second Affiliated Hospital , Nanjing Medical University , Nanjing , Jiangsu , China.
Xiao-Yan
XY
Ying
a State Key Lab of Reproductive Medicine , Nanjing Medical University , Nanjing , Jiangsu , P.R. China.
Dong
D
Zhang
D000255:Adenosine Triphosphate / Q000378:metabolism; D000782:Aneuploidy; D000818:Animals; D020090:Chromosome Segregation; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005260:Female; D005306:Fertilization; D019204:GTP-Binding Proteins / Q000378:metabolism; D055785:Gene Knockdown Techniques; D008540:Meiosis; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding
D003598:Cytoskeletal Proteins; C000622217:ELMOD2 protein, mouse; D000255:Adenosine Triphosphate; C485819:Arl2 protein, mouse; D019204:GTP-Binding Proteins
ATP; Arl2; Elmod2; GTPase-activating protein; meiosis; mitochondria; oocyte