Life-Span Extension by Axenic Dietary Restriction Is Independent of the Mitochondrial Unfolded Protein Response and Mitohormesis in Caenorhabditis elegans.
In Caenorhabditis elegans, a broad range of dietary restriction regimens extend life span to different degrees by separate or partially overlapping molecular pathways. One of these regimens, axenic dietary restriction, doubles the worm's life span but currently, almost nothing is known about the underlying molecular mechanism. Previous studies suggest that mitochondrial stress responses such as the mitochondrial unfolded protein response (UPRmt) or mitohormesis may play a vital role in axenic dietary restriction-induced longevity. Here, we provide solid evidence that axenic dietary restriction treatment specifically induces an UPRmt response in C elegans but this induction is not required for axenic dietary restriction-mediated longevity. We also show that reactive oxygen species-mediated mitohormesis is not involved in this phenotype. Hence, changes in mitochondrial physiology and induction of a mitochondrial stress response are not necessarily causal to large increases in life span.
Laboratory of Aging Physiology and Molecular Evolution, Biology Department, Ghent University, Belgium.
Huaihan
H
Cai
Laboratory of Aging Physiology and Molecular Evolution, Biology Department, Ghent University, Belgium.
Madina
M
Rasulova
Laboratory of Aging Physiology and Molecular Evolution, Biology Department, Ghent University, Belgium.
Lieselot
L
Vandemeulebroucke
Laboratory of Aging Physiology and Molecular Evolution, Biology Department, Ghent University, Belgium.
Lea
L
Meagher
Laboratory of Aging Physiology and Molecular Evolution, Biology Department, Ghent University, Belgium.
Caroline
C
Vlaeminck
Laboratory of Aging Physiology and Molecular Evolution, Biology Department, Ghent University, Belgium.
Ineke
I
Dhondt
Laboratory of Aging Physiology and Molecular Evolution, Biology Department, Ghent University, Belgium.
Bart P
BP
Braeckman
D000367:Age Factors; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D031204:Caloric Restriction; D008017:Life Expectancy; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D056811:Unfolded Protein Response
D017382:Reactive Oxygen Species
Axenic culture; Longevity; UPRmt