Supplemental description of Nyctotheroides pyriformis n. comb. (=Macrocytopharynxa pyriformis (Nie, 1932) Li et al. 2002) from frog hosts with consideration of the validity of the genus Macrocytopharynxa (Armophorea, Clevelandellida).
The morphological revisions of Macrocytopharynxa pyriformis (Nie, 1932) Li et al., 2002; collected from the rectum of Fejervarya limnocharis (=Rana limnocharis), are presented in this paper: (1) two surfaces of the organism are not identical - left side narrower and convex, right broader and flat or slightly concave; (2) infundibulum is large and well-developed with no "fold" or "plicature" present in the middle or posterior portion; (3) micronucleus is tiny and ovoid shaped and always embedded in the middle concavity of macronucleus, which can be well revealed by ammoniacal silver staining. Our phylogenetic analysis based on SSU-rDNA showed that M. pyriformis fell into the Nyctotheroides clade, within which four definite Nyctotheroides species were involved - N. cordiformis, N. deslierresae, N. parvus and N. hubeiensis. In combination with their morphological features, we discussed the reliability of using karyophore organelles or kinetal suture patterns as the generic taxonomic criteria. Besides, we considered that the genus Macrocytopharynxa is a junior synonym of Nyctotheroides and we transfer its type species to Nyctotheroides as Nyctotheroides pyriformis n. comb. The phylogenetic pattern of the family Nyctotheridae was also indicated in our work, but it will be necessary to analyze more species from fishes and reptiles before coming to a sound conclusion.
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Hubei Collaborative Innovation Centre for Freshwater Aquaculture, Wuhan 430070, China.
Ming
M
Li
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.
Can
C
Li
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.
J Norman
JN
Grim
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, Madrid 28040, Spain.
Francisco
F
Ponce-Gordo
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: gtwang@ihb.ac.cn.
Guitang
G
Wang
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Hong
H
Zou
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Wenxiang
W
Li
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Shangong
S
Wu
D000818:Animals; D001001:Anura / Q000469:parasitology*; D016798:Ciliophora / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D004275:DNA, Ribosomal / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D013045:Species Specificity
D004275:DNA, Ribosomal
Macrocytopharynxa; Morphology; Nyctotheroides; Nyctotherus; Phylogenetic analysis; SSU-rDNA