Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection.
The detection of pathogenic bacteria is essential in various fields, such as food safety, water environmental analysis, or clinical diagnosis. Although rapid and selective techniques have been achieved based on the fast and specific binding of recognitions elements and target, the sensitive detection of bacterial pathogens was limited by their low targets-binding efficiency. The three-dimensional (3D) nano-biointerface, compared with the two-dimensional (2D) flat substrate, has a much higher binding capacity, which can offer more reactive sites to bind with bacterial targets, resulting in a great improvement of detection sensitivity. Herein, a lectin functionalized ZnO nanorod (ZnO-NR) array has been fabricated and employed as a 3D nano-biointerface for Escherichia coli (E. coli) capture and detection by multivalent binding of concanavalin A (ConA) with polysaccharides on the cellular surface of E. coli. The 3D lectin functionalized ZnO-NR array-based assay shows reasonable detection limit and efficiently expanded linear range (1.0×103to 1.0×107cfumL-1) for pathogen detection. The platform has a potential for further applications and provides an excellent sensitivity approach for detection of pathogenic bacteria.
Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of the Chinese Academy of Science, Beijing 100039, China.
Laibao
L
Zheng
Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Yi
Y
Wan
Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Peng
P
Qi
Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Yan
Y
Sun
Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: zhangdun@qdio.ac.cn.
Dun
D
Zhang
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Qingdao Collaborative Innovation Center of Marine Science and Technology, Ocean University of China, Qingdao 266100, China.
Liangmin
L
Yu
D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D037102:Lectins / Q000737:chemistry*; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
D037102:Lectins; D011135:Polysaccharides, Bacterial; D003208:Concanavalin A; D015034:Zinc Oxide
3D nano-interface; Bacterial detection; Lectin functionalization; ZnO-NR arrays