Lipoic Acid Gold Nanoparticles Functionalized with Organic Compounds as Bioactive Materials.
Water soluble gold nanoparticles protected by lipoic acid were obtained and further functionalized by standard coupling reaction with 1-naphtylamine, 4-aminoantipyrine, and 4'-aminobenzo-15-crown-5 ether. Derivatives of lipoic acid with 1-naphtylamine, 4-aminoantipyrine, and 4'-aminobenzo-15-crown-5 ether were also obtained and characterized. All these were tested for their antimicrobial activity, as well as for their influence on mammalian cell viability and cellular cycle. In all cases a decreased antimicrobial activity of the obtained bioactive nanoparticles was observed as compared with the organic compounds, proving that a possible inactivation of the bioactive groups could occur during functionalization. However, both the gold nanoparticles as well as the functionalized bioactive nanosystems proved to be biocompatible at concentrations lower than 50 µg/mL, as revealed by the cellular viability and cell cycle assay, demonstrating their potential for the development of novel antimicrobial agents.
Department of Organic Chemistry, Biochemistry and Catalysis, University of Bucharest, 90-92 Panduri, 050663 Bucharest, Romania. oana_turcu@yahoo.com.
Ioana
I
Turcu
Department of Organic Chemistry, Biochemistry and Catalysis, University of Bucharest, 90-92 Panduri, 050663 Bucharest, Romania. zarafuirina@yahoo.fr.
Irina
I
Zarafu
Microbiology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Way, 060101 Bucharest, Romania. bmarcelica@yahoo.com.|Research Institute of the University of Bucharest-ICUB, 91-95 Spl. Independentei, 050095 Bucharest, Romania. bmarcelica@yahoo.com.
Marcela
M
Popa
Microbiology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Way, 060101 Bucharest, Romania. carmen_balotescu@yahoo.com.|Research Institute of the University of Bucharest-ICUB, 91-95 Spl. Independentei, 050095 Bucharest, Romania. carmen_balotescu@yahoo.com.
Mariana Carmen
MC
Chifiriuc
Microbiology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Way, 060101 Bucharest, Romania. cbleotu@yahoo.com.|Romanian Academy, Ştefan S. Nicolau Institute of Virology-IVN, 285 Mihai Bravu Avenue, Sector 3, PO 77, PO Box 201, 030304 Bucharest, Romania. cbleotu@yahoo.com.
Coralia
C
Bleotu
Institute of Physical Chemistry, 202 Spl. Independentei, 060021 Bucharest, Romania. danaculita@yahoo.co.uk.
Daniela
D
Culita
National Institute of Materials Physics, Laboratory of Atomic Structures and Defects in Advanced Materials, Atomistilor 105 bis, 077125 Magurele, Romania. cghica@infim.ro.
Corneliu
C
Ghica
Department of Organic Chemistry, Biochemistry and Catalysis, University of Bucharest, 90-92 Panduri, 050663 Bucharest, Romania. p_ionita@yahoo.co.uk.|Institute of Physical Chemistry, 202 Spl. Independentei, 060021 Bucharest, Romania. p_ionita@yahoo.co.uk.
Petre
P
Ionita
antimicrobial; cellular cycle; cytotoxicity; gold nanoparticles; lipoic acid; thioctic acid