CRISPR Meets CAR T-cell Therapy.
Using CRISPR/Cas9 technology, researchers have devised a method to deliver a CAR gene to a specific locus,TRAC, in T cells. This targeted approach yielded therapeutic cells that were more potent even at low doses; in a mouse model of acute lymphoblastic leukemia, they outperformed CAR T cells created with a randomly integrating retroviral vector.