Concentrate supplementation of a diet based on medium-quality grass silage for 4 weeks prepartum: Effects on cow performance, health, metabolic status, and immune function.
Because negative energy balance (EB) contributes to transition-period immune dysfunction in dairy cows, dietary management strategies should aim to minimize negative EB during this time. Prepartum diets that oversupply energy may exacerbate negative EB in early lactation, with detrimental effects on immune function. However, with lower body condition score (BCS) cows, it has been shown that offering concentrates in addition to a grass silage-based diet when confined during an 8-wk dry period resulted in increased neutrophil function in early lactation. The aim of this study was to examine if similar benefits occur when concentrate feeding was restricted to a 4-wk period prepartum. Twenty-six multiparous and 22 primiparous Holstein-Friesian cows were offered ad libitum access to medium-quality grass silage until 28 d before their predicted calving dates (actual mean of 32 d prepartum; standard deviation = 6.4). At this time multiparous cows had a mean BCS of 2.9 (standard deviation = 0.12) and primiparous cows a mean BCS of 3.0 (standard deviation = 0.14) on a 1 to 5 scale. Cows were then allocated in a balanced manner to 1 of 2 treatments (13 multiparous cows and 11 primiparous cows on each treatment): silage only (SO) or silage plus concentrates (S+C) until calving. Cows on SO were offered the same grass silage ad libitum. Cows on S+C were offered an ad libitum mixed ration of the same grass silage and additional concentrates in a 60:40 dry matter (DM) ratio, which provided a mean concentrate DM intake (DMI) of 4.5 kg/cow per d. After calving, all cows were offered a common mixed ration (grass silage and concentrates, 40:60 DM ratio) for 70 d postpartum. Offering concentrates in addition to grass silage during the 4 wk prepartum increased prepartum DMI (12.0 versus 10.1 kg/cow per d), EB (+40.0 versus +10.6 MJ/cow per d), and body weight (BW; 640 versus 628 kg), and tended to increase BCS (3.02 versus 2.97). However, postpartum DMI, milk yield, milk composition, BW change, BCS change, serum nonesterified fatty acid, and β-hydroxybutryrate concentrations, health, and corpus luteum measures were unaffected by treatment. The in vitro assays of neutrophil phagocytosis, neutrophil oxidative burst, and interferon gamma production, conducted on blood samples obtained at d 14 prepartum and d 3, 7, 14, and 21 postpartum, were unaffected by treatment. Primiparous cows had higher phagocytic fluorescence intensity at d 14 prepartum and d 3 and 7 postpartum; a higher percentage of neutrophils undergoing oxidative burst at d 3, 7, and 21 postpartum; and a higher oxidative burst fluorescence intensity at d 14 prepartum and d 7, 14, and 21 postpartum compared with multiparous cows. This suggests that neutrophil function of primiparous cows was less sensitive to the changes occurring during the transition period than that of multiparous cows. In conclusion, offering concentrates during the 4-wk period prepartum had no effect on postpartum DMI, milk yield, body tissue mobilization, EB, measures of neutrophil or lymphocyte function, health, or corpus luteum activity.
Sustainable Agri-Food Sciences Division, Agri-Food and Biosciences Institute, Large Park, Hillsborough, BT26 6DR, United Kingdom; School of Biological Sciences, Institute for Global Food Security, Queen's University Belfast, 18-30 Malone Road, Belfast, BT9 5BN, United Kingdom. Electronic address: mark.little@afbini.gov.uk.
M W
MW
Little
School of Biological Sciences, Institute for Global Food Security, Queen's University Belfast, 18-30 Malone Road, Belfast, BT9 5BN, United Kingdom.
N E
NE
O'Connell
Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stoney Road, Belfast, BT4 3SD, United Kingdom.
M D
MD
Welsh
School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
F J
FJ
Mulligan
Sustainable Agri-Food Sciences Division, Agri-Food and Biosciences Institute, Large Park, Hillsborough, BT26 6DR, United Kingdom.
C P
CP
Ferris
D000821:Animal Feed; D000818:Animals; D002417:Cattle; D004032:Diet; D002149:Energy Intake; D004734:Energy Metabolism; D005260:Female; D007774:Lactation / Q000502:physiology*; D008892:Milk; D009504:Neutrophils / Q000502:physiology*; D010298:Parity; D006109:Poaceae; D049590:Postpartum Period; D011247:Pregnancy; D012820:Silage; D013997:Time Factors
dairy cow; immunity; metabolic status; transition period