[Periodontal inflammation affects the mechanical and immune barrier functions of mice gut].
OBJECTIVE
To explore the effects of periodontal inflammation on the functions of gut barrier (ecological barrier, mechanical barrier, and immune barrier) in mice.


METHODS
Twenty male C57BL/6J mice were randomly divided into perio-dontitis (P) or control (C) groups. The P group was subjected under a 10-day ligation with Porphyromonas gingivalis to induce periodontitis, whereas the C group was ligated with sham. Maxillae were obtained to assess alveolar bone loss. The phylogenetic structure and diversity of microbial communities in the gut were analyzed by 16s rRNA pyrosequencing. Immunohisto-chemical analysis was performed to determine the expressions of occludin, claudin2, and NOD2 in the ileum.


RESULTS
Com-pared with the C group, the P group displayed significant alveolar bone loss (P<0.001). In addition, no significant influence on the main phyla and genus Parabacteroides of the two groups was observed (P>0.05). However, the ileum of the P group showed significantly upregulated occludin, claudin2, and NOD2 (P=0.039, P=0.011, and P=0.039, respectively).


CONCLUSIONS
Periodontal inflammation influences to some extent the mechanical and immune barrier functions of the mice gut.  .
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China.
Xie
X
Meilian
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Guangzhou Medical University, Guangzhou 510150, China.
Yu
Y
Ting
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China.
Zhuo
Z
Ying
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China.
Huang
H
Xin
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China.
Xie
X
Baoyi
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China;Dept. of Periodontology, Hangzhou Dental Hospital, Savaid Medical School, University of Chinese Academy of Sciences, Hangzhou 310006, China.
Xuan
X
Dongying
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China;Dept. of Periodontology, Hangzhou Dental Hospital, Savaid Medical School, University of Chinese Academy of Sciences, Hangzhou 310006, China.
Zhang
Z
Jincai
D016301:Alveolar Bone Loss; D000818:Animals; D007249:Inflammation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010518:Periodontitis; D010802:Phylogeny; D016966:Porphyromonas gingivalis; D012336:RNA, Ribosomal, 16S
D012336:RNA, Ribosomal, 16S
gut microbiota; inflammatory bowel diseases; mechanical barrier; periodontitis