You are what you get from your fungi: nitrogen stable isotope patterns in Epipactis species.
Partially mycoheterotrophic plants are enriched in 13 C and 15 N compared to autotrophic plants. Here, it is hypothesized that the type of mycorrhizal fungi found in orchid roots is responsible for variation in 15 N enrichment of leaf tissue in partially mycoheterotrophic orchids.



The genus Epipactis was used as a case study and carbon and nitrogen isotope abundances of eight Epipactis species, fungal sporocarps of four Tuber species and autotrophic references were measured. Mycorrhizal fungi were identified using molecular methods. Stable isotope data of six additional Epipactis taxa and ectomycorrhizal and saprotrophic basidiomycetes were compiled from the literature.



The 15 N enrichment of Epipactis species varied between 3·2 ± 0·8 ‰ ( E. gigantea ; rhizoctonia-associated) and 24·6 ± 1·6 ‰ ( E. neglecta ; associated with ectomycorrhizal ascomycetes). Sporocarps of ectomycorrhizal ascomycetes (10·7 ± 2·2 ‰) were significantly more enriched in 15 N than ectomycorrhizal (5·2 ± 4·0 ‰) and saprotrophic basidiomycetes (3·3 ± 2·1 ‰).



As hypothesized, it is suggested that the observed gradient in 15 N enrichment of Epipactis species is strongly driven by 15 N abundance of their mycorrhizal fungi; i.e. ɛ 15 N in Epipactis spp. associated with rhizoctonias < ɛ 15 N in Epipactis spp. with ectomycorrhizal basidiomycetes < ɛ 15 N in Epipactis spp. with ectomycorrhizal ascomycetes and basidiomycetes < ɛ 15 N in Epipactis spp. with ectomycorrhizal ascomycetes.
Laboratory of Isotope Biogeochemistry, Bayreuth Center of Ecology and Environmental Research (BayCEER), University of Bayreuth, 95440 Bayreuth, Germany.
Julienne M-I
JM
Schiebold
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.|Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK.
Martin I
MI
Bidartondo
Deutsche Gesellschaft für Mykologie (German Mycological Society), Kirchl 78, 94545 Hohenau, Germany.
Peter
P
Karasch
Naturalis Biodiversity Center, Leiden, the Netherlands.
Barbara
B
Gravendeel
Laboratory of Isotope Biogeochemistry, Bayreuth Center of Ecology and Environmental Research (BayCEER), University of Bayreuth, 95440 Bayreuth, Germany.
Gerhard
G
Gebauer
D002247:Carbon Isotopes / Q000032:analysis; D005658:Fungi / Q000737:chemistry; D038821:Mycorrhizae / Q000737:chemistry*; D009587:Nitrogen Isotopes / Q000032:analysis*; D029595:Orchidaceae / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes
Ascomycetes; Epipactis; Orchidaceae; Tuber; basidiomycetes; carbon; mycorrhiza; nitrogen; partial mycoheterotrophy; stable isotopes