Association of Nitric Oxide Synthase2 gene polymorphisms with leprosy reactions in northern Indian population.
The pathogen Mycobacterium leprae causes leprosy that affects mainly skin and nerves. Polymorphisms of certain genes are substantiated to be associated with the susceptibility/resistance to leprosy. The present investigation addressed the association of Nitric Oxide Synthase2 gene polymorphisms and leprosy in a population from northern part of India. A total of 323 leprosy cases and 288 healthy controls were genotyped for four NOS2 promoter variants (rs1800482, rs2779249, rs8078340 and rs2301369) using FRET technology in Real Time PCR. None of these SNPs in promoter sites was associated with susceptibility/resistance to leprosy. NOS2 rs1800482 was found to be monomorphic with GG genotype. However, NOS2-1026T allele was observed to be in higher frequency with leprosy cases (BL and LL) who were not suffering from any reactional episodes compared to cases with ENL reaction {OR=0.30, 95% CI (0.10-0.86), p=0.024}. NOS2-1026GT genotype was more prevalent in cases without reaction (BT, BB and BL) compared to RR reactional patients {OR=0.38, 95% CI (0.17-0.86), p=0.02}. Although haplotype analysis revealed that no haplotype was associated with leprosy susceptibility/resistance with statistical significance, GTG haplotype was noted to be more frequent in healthy controls. These SNPs are observed to be in linkage disequilibrium. Although, these SNPs are not likely to influence leprosy vulnerability, -1026G>T SNP was indicated to have noteworthy role in leprosy reactions.
Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: amitbiotech83@gmail.com.
Amit
A
Dubey
Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: sanjay_sls2000@yahoo.com.
Sanjay Kumar
SK
Biswas
Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: sinhaekata@rediffmail.com.
Ekata
E
Sinha
Clinical Division National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: drjkna@yahoo.com.
Joy Kumar
JK
Chakma
Clinical Division National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: rajushikamal@rediffmail.com.
Raj
R
Kamal
Clinical Division National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: mamtaarora_aroram@rediffmail.com.
Mamta
M
Arora
Clinical Division National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: drharishkumarsagar@gmail.com.
Harish
H
Sagar
Histopathlogy Division National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: mohannatrajan13@gmail.com.
Mohan
M
Natarajan
School of Biotechnology, Jiwaji University, Gwalior 474001, India. Electronic address: sameerbhagyawant@yahoo.com.
Sameer S
SS
Bhagyawant
Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. Electronic address: mohantysk@icmr.org.in.
Keshar Kunja
KK
Mohanty
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D007194:India; D007918:Leprosy / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D009166:Mycobacterium leprae / Q000472:pathogenicity / Q000502:physiology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
Heterozygous genotype; Leprosy; NOS2; Polymorphism; Reactions