Bacterial fauna associating with chironomid larvae from lakes of Bengaluru city, India - A 16s rRNA gene based identification.
Chironomid larvae that inhabit in aquatic sediments play an important role as vector for bacterial pathogens. Its life cycle consists of four stages i.e. eggs, larvae, pupae and adult. In the present study we identified bacterial species associated with whole larvae of chironomids from 11 lake sediments of Bangalore region using 16s rRNA gene Sanger sequencing. We found that larvae from all lake sediments associated with bacterial species which include key pathogens. Totally we identified 65 bacterial isolates and obtained GenBank accession numbers (KX980423 - KX980487). Phylogenetic tree constructed using MEGA 7 software and tree analysis highlight the predominant bacterial community associated with larvae which includeEnterobacteriaceae(43.08%; 28 isolates) andAeromonas(24.62%; 16 isolates),Shewanella,Delftia,Bacillus(6.15%; 4 isolates each),Pseudomonas(4.62%; 3 isolates) andExiguobacterium(3.08%; 2 isolates). Current findings state that among bacterial populationAeromonas,EnterobacterandEscherichiawith serotypes are commonly associated with larvae in maximum lake points. In other handVibrio,Pseudomonas,Klebsiella,Shigella,Bacillus, and other bacterial species were identified moderately in all lakes. Interestingly, we identified first timeShigellaGram negative, rod shaped pathogenic organism ofEnterobacteriaceaeandRheinheimeraGram negative, rod shaped organism associating chironomid larvae.
Eurofins Genomics India Pvt. Ltd, Bengaluru 560048, Karnataka, India.
Ramprasad
R
Kuncham
Eurofins Genomics India Pvt. Ltd, Bengaluru 560048, Karnataka, India.
Thiyagarajan
T
Sivaprakasam
Eurofins Genomics India Pvt. Ltd, Bengaluru 560048, Karnataka, India.
R
R
Puneeth Kumar
Eurofins Genomics India Pvt. Ltd, Bengaluru 560048, Karnataka, India.
P
P
Sreenath
Eurofins Genomics India Pvt. Ltd, Bengaluru 560048, Karnataka, India.
Ravi
R
Nayak
School of Biotechnology, Dr. G.R. Damodaran College of Science, Coimbatore 641014, Tamilnadu, India.
Tha
T
Thayumanavan
Department of Chemistry, JNTUACE, Pulivendula 516390, Andhra Pradesh, India.
Gopireddy V
GV
Subba Reddy
16s rRNA gene; Bacterial species; Chironomid larvae; MEGA 7 software; Phylogenetic tree; Sanger sequencing