Synthesis and evaluation of a tag-free photoactive phospho-ceramide analogue-1 (PCERA-1) probe to study immunomodulation in macrophages.
Phospho-ceramide analogue-1 (PCERA-1), a synthetic analogue of ceramide-1-phosphate (C1P), has been previously shown to act as a potent modulator of macrophage activity and inflammation. We have developed an efficient synthesis of PCERA-1 from readily available starting materials, and designed and prepared derivatives of this analogue, including a photoaffinity probe to tag and identify putative proteins that bind PCERA-1.
Dept. of Chemistry and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel. meijler@bgu.ac.il.
Rambabu
R
Dandela
Dept. of Chemistry and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel. meijler@bgu.ac.il.
Roi
R
Mashiach
Dept. of Chemistry and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel. meijler@bgu.ac.il.
Raju
R
Adepu
Dept. of Chemistry and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel. meijler@bgu.ac.il.
Rachel
R
Gregor
Department of Biochemistry & Molecular Biology, Life Sciences Institute, Tel-Aviv University, Tel-Aviv 69978, Israel. tsaffyz@tauex.tau.ac.il and The Triangle Regional Research and Development Center, P.O. Box 2167, Kfar Qari', 30075, Israel.
Muhammad
M
Athamna
Department of Biochemistry & Molecular Biology, Life Sciences Institute, Tel-Aviv University, Tel-Aviv 69978, Israel. tsaffyz@tauex.tau.ac.il.
Efrat
E
Zecharia
Department of Biochemistry & Molecular Biology, Life Sciences Institute, Tel-Aviv University, Tel-Aviv 69978, Israel. tsaffyz@tauex.tau.ac.il.
Orna
O
Ernst
Department of Biochemistry & Molecular Biology, Life Sciences Institute, Tel-Aviv University, Tel-Aviv 69978, Israel. tsaffyz@tauex.tau.ac.il.
Tsaffrir
T
Zor
Dept. of Chemistry and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel. meijler@bgu.ac.il.
Michael M
http://orcid.org/0000-0001-7095-1467
MM
Meijler
D000818:Animals; D002518:Ceramides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D056747:Immunomodulation / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D055668:Photochemical Processes; D000067996:RAW 264.7 Cells; D013329:Structure-Activity Relationship
D002518:Ceramides; D008070:Lipopolysaccharides; D015335:Molecular Probes; C541330:PCERA-1