Interlaboratory trial for the measurement of total cobalt in equine urine and plasma by ICP-MS.
Cobalt is an essential mineral micronutrient and is regularly present in equine nutritional and feed supplements. Therefore, cobalt is naturally present at low concentrations in biological samples. The administration of cobalt chloride is considered to be blood doping and is thus prohibited. To control the misuse of cobalt, it was mandatory to establish an international threshold for cobalt in plasma and/or in urine. To achieve this goal, an international collaboration, consisting of an interlaboratory comparison between 5 laboratories for the urine study and 8 laboratories for the plasma study, has been undertaken. Quantification of cobalt in the biological samples was performed by inductively coupled plasma-mass spectrometry (ICP-MS). Ring tests were based on the analysis of 5 urine samples supplemented at concentrations ranging from 5 up to 500 ng/mL and 5 plasma samples spiked at concentrations ranging from 0.5 up to 25 ng/mL. The results obtained from the different laboratories were collected, compiled, and compared to assess the reproducibility and robustness of cobalt quantification measurements. The statistical approach for the ring test for total cobalt in urine was based on the determination of percentage deviations from the calculated means, while robust statistics based on the calculated median were applied to the ring test for total cobalt in plasma. The inter-laboratory comparisons in urine and in plasma were successful so that 97.6% of the urine samples and 97.5% of the plasma samples gave satisfactory results. Threshold values for cobalt in plasma and urine were established from data only obtained by laboratories involved in the ring test. Copyright © 2017 John Wiley & Sons, Ltd.
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Marie-Agnes
http://orcid.org/0000-0002-5179-9612
MA
Popot
Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Sha Tin, NT, Hong Kong, China.
Emmie N M
ENM
Ho
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Natali
N
Stojiljkovic
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Florian
F
Bagilet
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Pierre
P
Remy
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Pascal
P
Maciejewski
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Benoit
http://orcid.org/0000-0001-8306-5923
B
Loup
Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Sha Tin, NT, Hong Kong, China.
George H M
GHM
Chan
School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Sabine
S
Hargrave
School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Rick M
RM
Arthur
Racing Chemistry Laboratory, ChemCentre, PO Box 1250, Bentley Delivery Centre, Western Australia, 6983, Australia.
Charlie
C
Russo
Racing Chemistry Laboratory, ChemCentre, PO Box 1250, Bentley Delivery Centre, Western Australia, 6983, Australia.
James
J
White
Sport and Specialised Analytical Services, LGC Ltd, Newmarket Road, Fordham, Cambridgeshire, CB7 5WW, UK.
Pamela
P
Hincks
Sport and Specialised Analytical Services, LGC Ltd, Newmarket Road, Fordham, Cambridgeshire, CB7 5WW, UK.
Clive
C
Pearce
Racing Analytical Services Ltd, 400 Epsom Road, Flemington, Victoria, 3031, Australia.
George
G
Ganio
Racing Analytical Services Ltd, 400 Epsom Road, Flemington, Victoria, 3031, Australia.
Paul
P
Zahra
Racing Analytical Services Ltd, 400 Epsom Road, Flemington, Victoria, 3031, Australia.
David
D
Batty
Racing Science Centre, Cooksley St, Hamilton, QLD, 4007, Australia.
Mark
M
Jarrett
Canadian Pari-Mutuel Agency, 1130 Morrison Dr. Ottawa, Ontario, K2H 9N, Canada.|Canadian Food Inspection Agency, 960 Carling Av. Bldg#22 C.E.F. Ottawa, Ontario, K1A 0Y9.
Lydia
L
Brooks
Canadian Pari-Mutuel Agency, 1130 Morrison Dr. Ottawa, Ontario, K2H 9N, Canada.|Canadian Food Inspection Agency, 960 Carling Av. Bldg#22 C.E.F. Ottawa, Ontario, K1A 0Y9.
Lise-Anne
LA
Prescott
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Ludovic
http://orcid.org/0000-0002-0864-1959
L
Bailly-Chouriberry
Laboratoire des Courses Hippiques (LCH), 15 rue de Paradis, 91370, Verrières-le-Buisson, France.
Yves
Y
Bonnaire
Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Sha Tin, NT, Hong Kong, China.
Terence S M
TSM
Wan
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D003035:Cobalt / Q000032:analysis* / Q000737:chemistry / Q000652:urine*; D019587:Dietary Supplements / Q000032:analysis*; D006736:Horses; D013058:Mass Spectrometry / Q000379:methods*; D010949:Plasma / Q000737:chemistry*; D015203:Reproducibility of Results
D003035:Cobalt; C018021:cobaltous chloride
inductively coupled plasma-mass spectrometry; interlaboratory reproducibility; plasma; total cobalt; urine