Digoxin reduces the mutagenic effects of Mitomycin C in human and rodent cell lines.
Digoxin is a drug widely used to treat heart failure and studies have demonstrated its potential as anticancer agent. In addition, digoxin presents the potential to interact with a series of other compounds used in medicine. The aim of the present study was to evaluate in vitro the cytotoxicity, genotoxicity and mutagenicity of digoxin and its potential to interact with the mutagen Mitomycin C (MMC). The cytotoxicity of digoxin was assessed by employing the MTT method and the comet assay was performed to assess the genotoxicity of this medicine in CHO-K1 and HeLa cell lines. Besides, the cytokinesis-block micronucleus assay was performed to assess the mutagenicity and the antimutagenicity of this drug. The Ames assay was also performed with TA98 and TA100 strains of S. typhimurium. Results showed that digoxin was cytotoxic, genotoxic and mutagenic for HeLa and CHO-K1 cell lines at concentrations many times higher than those observed in human therapeutic conditions. Nevertheless, an antimutagenic effect against the mutagen MMC was observed on both cell lines in concentrations near those used therapeutically in humans. This chemoprotective effect observed is an interesting finding that should be better explored regarding its impact in anticancer chemotherapy.
Laboratório de Biologia Celular e Mutagênese (LaBCeM), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-506, Brazil.|Núcleo de Pesquisa em Química Biológica (NQBio), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-296, Brazil.
Júlia Teixeira
JT
de Oliveira
Laboratório de Biologia Celular e Mutagênese (LaBCeM), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-506, Brazil.|Núcleo de Pesquisa em Química Biológica (NQBio), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-296, Brazil.
Maria C da Silva
MCDS
Barbosa
Laboratório de Biologia Celular e Mutagênese (LaBCeM), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-506, Brazil.|Núcleo de Pesquisa em Química Biológica (NQBio), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-296, Brazil.
Luiz F
LF
de Camargos
Laboratório de Biologia Celular e Mutagênese (LaBCeM), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-506, Brazil.
Isabella Viana Gomes
IVG
da Silva
Núcleo de Pesquisa em Química Biológica (NQBio), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-296, Brazil.
Fernando de Pilla
FP
Varotti
Serviço de Biologia Celular (SBC), Fundação Ezequiel Dias (FUNED), Belo Horizonte, MG, 30510-010, Brazil.
Luciana M
LM
da Silva
Departamento de Zootecnia (DZOO), Universidade Federal de São João del Rei (UFSJ), São João del Rei, MG, 36301-160, Brazil.
Leonardo Marmo
LM
Moreira
Departamento de Ciências Naturais (DCNAT), Universidade Federal de São João del Rei (UFSJ), São João del Rei, MG, 36301-160, Brazil.
Juliana Pereira
JP
Lyon
Laboratório de Biologia Celular e Mutagênese (LaBCeM), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-506, Brazil.
Vanessa J da Silva Vieira
VJDSV
Dos Santos
Laboratório de Biologia Celular e Mutagênese (LaBCeM), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-506, Brazil. fabiosantos@ufsj.edu.br.|Núcleo de Pesquisa em Química Biológica (NQBio), Universidade Federal de São João del Rei (UFSJ), Divinópolis, MG, 35501-296, Brazil. fabiosantos@ufsj.edu.br.
Fabio Vieira
http://orcid.org/0000-0003-0389-1737
FV
Dos Santos
Antimutagenicity; Desmutagen; Genotoxicity; Mutagenicity