Development of an up-grading process to produce MLM structured lipids from sardine discards.
The aim of the work was to produce MLM structured lipids with caprylic acid (M) as medium chain fatty acid located at the external bonds of the glycerol backbone and concentrated polyunsaturated fatty acids (L) from sardine discards (Sardine pilchardus) in the central bond of the glycerol. To that end, the following steps were conducted: (i) fish oil extraction, (ii) Omega-3 free fatty acids (FFA) concentration (low temperature winterization), (iii) two-steps enzymatic esterification and (iv) triacylglycerols (TAG) purification (liquid column chromatography). The resultant purified triacylglycerols accomplished with the oxidative state (peroxide and anisidine value, PV and AV) required for refined oils. As enzymatic treatment, Omega-3 concentrate FFA (Omega-3>600mg Omega-3 per g oil) were esterified with dicaprylic glycerol employing Novozyme 435. This process presented high regioselectivity, with ∼80mol% of concentrated fatty acids esterified at the sn-2 position.
Department of Chemical Engineering, University of Granada, Granada, Spain. Electronic address: rocio_morales@ugr.es.
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Morales-Medina
Department of Chemical Engineering, University of Granada, Granada, Spain.
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Munio
Department of Chemical Engineering, University of Granada, Granada, Spain.
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Guadix
Department of Chemical Engineering, University of Granada, Granada, Spain.
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Guadix
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry*; D005395:Fish Oils / Q000737:chemistry*; D005399:Fishes; D008055:Lipids / Q000737:chemistry*; D017747:Seafood / Q000032:analysis*
D005227:Fatty Acids; D005395:Fish Oils; D008055:Lipids
Esterification; Fatty acids regiodistribution; Fish oil extraction; Liquid column chromatography; Low temperature winterization; Structured lipids