Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars.
The shiitake mushroom (Lentinulaedodes) is one of the most popular edible mushrooms in the world and has attracted attention for its value in medicinal and pharmacological uses. With recent advanced research and techniques, the agricultural cultivation of the shiitake mushroom has been greatly increased, especially in East Asia. Additionally, demand for the development of new cultivars with good agricultural traits has been greatly enhanced, but the development processes are complicated and more challenging than for other edible mushrooms. In this study, we developed 44 novel polymorphic simple sequence repeat (SSR) markers for the determination of shiitake mushroom cultivars based on a whole genome sequencing database ofL. edodes. These markers were found to be polymorphic and reliable when screened in 23 shiitake mushroom cultivars. For the 44 SSR markers developed in this study, the major allele frequency ranged from 0.13 to 0.94; the number of genotypes and number of alleles were each 2-11; the observed and expected heterozygosity were 0.00-1.00 and 0.10-0.90, respectively; and the polymorphic information content value ranged from 0.10 to 0.89. These new markers can be used for molecular breeding, the determination of cultivars, and other applications.
Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea. hoasis82@chungbuk.ac.kr.|Department of Biology, Chungbuk National University, Cheongju 28644, Korea. hoasis82@chungbuk.ac.kr.
Hwa-Yong
HY
Lee
Department of Biology, Chungbuk National University, Cheongju 28644, Korea. sooym21@naver.com.
Suyun
S
Moon
Department of Forest Genetic Resources, National Institute of Forest Science, Suwon 16631, Korea. shim104@korea.kr.
Donghwan
D
Shim
Theragen Etex Bio Institute, Suwon 16229, Korea. changpyo.hong@theragenetex.com.
Chang Pyo
CP
Hong
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea. leeyi22@chungbuk.ac.kr.
Yi
Y
Lee
Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea. koocdm@chungbuk.ac.kr.
Chang-Duck
CD
Koo
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea. jwchung73@chungbuk.ac.kr.
Jong-Wook
JW
Chung
Department of Biology, Chungbuk National University, Cheongju 28644, Korea. hjryu96@gmail.com.
Hojin
H
Ryu
shiitake mushroom; simple sequence repeat (SSR) marker; whole genome sequencing