Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase.
We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase (TDG) activity through cyclic enzymatic repairing-mediated dual-signal amplification. This method exhibits excellent sensitivity with a detection limit of 5.6 × 10-7U μL-1, and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells.
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn tangb@sdnu.edu.cn.
Li-Juan
LJ
Wang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn tangb@sdnu.edu.cn.
Zi-Yue
ZY
Wang
Nantou High School Shenzhen, Shenzhen, 518052, China.
Qianyi
Q
Zhang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn tangb@sdnu.edu.cn.
Bo
http://orcid.org/0000-0002-8712-7025
B
Tang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn tangb@sdnu.edu.cn.
Chun-Yang
http://orcid.org/0000-0002-8010-1981
CY
Zhang
D015374:Biosensing Techniques; D006367:HeLa Cells; D006801:Humans; D008163:Luminescent Measurements; D061986:MCF-7 Cells; D044502:Thymine DNA Glycosylase / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
D044502:Thymine DNA Glycosylase