Autohydrolysis pretreatment assessment in ethanol production from agave bagasse.
The aim of the present work was to assess the autohydrolysis pretreatment of Agave tequilana bagasse for ethanol production. The pretreatment was conducted using a one-liter high pressure Parr reactor under different severity factors (SF) at a 1:6w/v ratio (solid:liquid) and 200rpm. The solids obtained under the selected autohydrolysis conditions were subjected to enzymatic hydrolysis with a commercial cellulase cocktail, and the enzymatic hydrolysate was fermented using Saccharomyces cerevisiae. The results obtained from the pretreatment process showed that the glucan content in the pretreated solid was mostly preserved, and an increase in the digestibility was observed for the case with a SF of 4.13 (190°C, 30min). Enzymatic hydrolysis of the pretreated solids showed a yield of 74.3%, with a glucose concentration of 126g/L, resulting in 65.26g/L of ethanol after 10h of fermentation, which represent a 98.4% conversion according to the theoretical ethanol yield value.
Departamento de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico. Electronic address: leopoldo.rios@uadec.edu.mx.
Leopoldo J
LJ
Rios-González
Departamento de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico.
Thelma K
TK
Morales-Martínez
Departamento de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico.
María F
MF
Rodríguez-Flores
Departamento de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico.
José A
JA
Rodríguez-De la Garza
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Saltillo, Saltillo, Coahuila, Mexico.
David
D
Castillo-Quiroz
Facultad de Ingeniería Química, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, Mexico.
Agustín J
AJ
Castro-Montoya
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Mor. 62250, Mexico.
Alfredo
A
Martinez
D027882:Agave; D002480:Cellulase; D002482:Cellulose; D000431:Ethanol; D005285:Fermentation; D006868:Hydrolysis
D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D002480:Cellulase
Agave tequilana bagasse; Autohydrolysis pretreatment; Ethanol