Role of Ketotifen on metabolic profiles, inflammation and oxidative stress in diabetic rats.
We aim to explore effects of Ketotifen on metabolic profiles, inflammation and oxidative stress. Sprague Dawley (SD) male rats were randomly divided into normal control group (NC) and experimental groups, and experimental group rats were fed with high-sugar and fat diet for 6 weeks. Then, experimental group rats were divided into diabetes group (DM) and ketotifen treatment group (KT). KT group was given ketotifen via Intragastric for 8 weeks with the dosage of 0.09 mg/kg/d. Fasting plasma glucose (FPG) was measured using glucose oxidase-phenol amino phenazone method. Fasting insulin (FINS), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were tested by enzyme-linked immunosorbent assay. Malondialdehyde (MDA) and superoxide dismutase (SOD) were quantified by spectrophotometer method. Before Ketotifen administration, compared with NC group, DM and KT groups showed significantly high levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α and MDA, and lower levels of HDL and SOD (All p <0.05). After 4 weeks of Ketotifen administration, levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α in KT group decreased significantly, and levels of HDL and SOD elevated significantly (All p <0.05). After 8 weeks of Ketotifen administration, levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α and MDA in KT group decreased more obviously, and levels of HDL and SOD elevated significantly further (All p <0.05). Ketotifen improved metabolic profiles, and ameliorated status of inflammation and oxidative stress.
Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215000, People's Republic of China.|Department of Endocrinology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Zimiao
Z
Chen
Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Hongwei
H
Sun
Department of Hand Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Jian
J
Wang
Department of Endocrinology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Liansong
L
Ni
Department of Endocrinology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Xuejiang
X
Gu
Department of Endocrinology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Shengjie
S
Ge
Department of Neurosurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Qiong
Q
Chen
Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People's Republic of China.
Liangshan
L
Mu
Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215000, People's Republic of China.
Xingbo
X
Cheng
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D007249:Inflammation / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D015850:Interleukin-6 / Q000097:blood; D007665:Ketotifen / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D055442:Metabolome / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D007328:Insulin; D015850:Interleukin-6; D008315:Malondialdehyde; D007665:Ketotifen
Diabetes mellitus; Dyslipidemia; Inflammation; Ketotifen; Mast cell