Fibrin scaffold enhances function of insulin producing cells differentiated from human umbilical cord matrix-derived stem cells.
Tissue engineering is a new strategy which proposed to treat numerous human diseases nowadays. Three dimensional (3D) scaffolds fill the gap between two dimensional cell culture (2D) and animal tissues through mimicking the environmental behaviors surrounding the cells. In this study, hUCMs into insulin producing cells in fibrin scaffold were differentiated compare to conventional culture condition. Differentiation rate was estimated by real time PCR, immunocytochemistry (ICC) and the chemiluminesence (CLIA) and enzyme immunoassay (EIA). Real time PCR's results showed an increasing expression in NKX2.2, PDX1 and INS (producing the hormone insulin) genes in fibrin scaffold. Furthermore ICC analysis exhibited that insulin and pro-insulin proteins were more in fibrin scaffolds. CLIA and EIA on insulin and C peptide secretion indicated that both of groups were sensitive to the glucose challenge test but significant higher response was observed in fibrin scaffold (6.5 fold in 3D, 1.8 fold in 2D culture). It could be concluded that differentiation of hUCM cells into insulin producing cells in fibrin scaffold 3D culture system is much more efficient than 2D conventional culture system.
Department of Anatomy, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran.
Fatemeh
F
Seyedi
Stem Cell Research Lab., Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Alireza
A
Farsinejad
Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; Afzal Research Institute, Kerman, Iran. Electronic address: nnematollahi@kmu.ac.ir.
Seyed Noureddin
SN
Nematollahi-Mahani
D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics*; D005337:Fibrin / Q000737:chemistry / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000096:biosynthesis; D006801:Humans; D007328:Insulin / Q000096:biosynthesis* / Q000235:genetics; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D009687:Nuclear Proteins; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D015534:Trans-Activators / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis; D014470:Umbilical Cord / Q000166:cytology / Q000378:metabolism; D029961:Zebrafish Proteins
D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D007328:Insulin; C000715631:NKX2-2 protein, human; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D029961:Zebrafish Proteins; C000715630:nkx2.2b protein, zebrafish; C084010:pancreatic and duodenal homeobox 1 protein; D005337:Fibrin
3D culture; Fibrin scaffold; Human umbilical cord matrix-derived mesenchymal cells (hUCMs); Insulin producing cell (IPC)