Differential dose-dependent effects of zinc oxide nanoparticles on oxidative stress-mediated pancreatic β-cell death.
OBJECTIVE
To study the effects of zinc oxide nanoparticles (ZON) on oxidative stress-mediated pancreatic β-cell death.


METHODS
Cellular uptake of ZON, effects on antioxidant factors and apoptosis were studied.


RESULTS
ZON get internalized by endocytosis and increase intracellular zinc ion levels. ZON treatment (30 and 100 μg/ml) to RIN5f cells resulted in cytotoxicity, oxidative stress and apoptosis. ZON (1, 3, 10 μg/ml, subcytotoxic concentrations) increased super oxide dismutase activity and levels of reduced glutathione in RIN5f cells. Furthermore, ZON (subcytotoxic concentrations) protected RIN5f cells from H2O2-induced oxidative stress as evidenced by reduced reactive oxygen species levels; increased super oxide dismutase activity and glutathione levels; and reduced apoptotic death.


CONCLUSIONS
ZON (subcytotoxic concentrations) protect pancreatic β cells from oxidative-stress-mediated cell death.
Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India.
Swati C
SC
Asani
Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India.
Rinku D
RD
Umrani
Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, Maharashtra, India.
Kishore M
KM
Paknikar
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D004705:Endocytosis / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010316:Particle Size; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology*
D000975:Antioxidants; D007004:Hypoglycemic Agents; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D005978:Glutathione; D015034:Zinc Oxide
cellular uptake; lysosomal co-localization; oxidative stress protection; pancreatic β cells; zinc oxide nanoparticles