Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve.
Significant progress has been made for tissue imaging of proteins using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS). These advancements now facilitate mapping of a wide range of proteins, peptides, and post-translational modifications in a wide variety of tissues; however, the use of MALDI IMS to detect proteins from cardiac tissue is limited. This review discusses the most recent advances in protein imaging and demonstrates application to cardiac tissue, including the heart valve. Protein imaging by MALDI IMS allows multiplexed histological mapping of proteins and protein components that are inaccessible by antibodies and should be considered an important tool for basic and clinical cardiovascular research. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.
Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, USA; Medical University of South Carolina Proteomics Center, Medical University of South Carolina, Charleston, USA. Electronic address: angelp@musc.edu.
Peggi M
PM
Angel
Department of Pediatrics and Cell Development and Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
H Scott
HS
Baldwin
Children's Hospital of New Orleans, LSU Health Science Center, New Orleans, LA, USA.
Danielle
D
Gottlieb Sen
Department of Cardiovascular Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
Yan Ru
YR
Su
Department of Cardiac Surgery, Boston Children's Hospital & Harvard Medical School, Boston, MA, USA.
John E
JE
Mayer
Division of Pediatric Cardiac Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.
David
D
Bichell
Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, USA; Medical University of South Carolina Proteomics Center, Medical University of South Carolina, Charleston, USA.
Richard R
RR
Drake
D006351:Heart Valves / Q000378:metabolism*; D006801:Humans; D011499:Protein Processing, Post-Translational / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D011506:Proteins
Cardiac; Cardiovascular; Extracellular matrix; Imaging mass spectrometry; Protein imaging; Semilunar valve