Next-generation Sequencing for microRNA Profiling: MicroRNA-21-3p Promotes Oral Cancer Metastasis.
Dysfunctional microRNAs (miRNAs) play a crucial role in oral squamous cell carcinoma (OSCC) progression. In the present study, we performed next-generation sequencing for miRNA profiling of the OSCC tissues and corresponding adjacent normal tissues in two patients with OSCC. We observed that 45 miRNAs were substantially up-regulated and 17 miRNAs were down-regulated in OSCC tissues. Since information on the biological role of miR-21-3p (passenger strand) in OSCC is limited, the expression levels of miR-21-3p were further evaluated in 95 OSCC tissue samples by a stem-loop real-time polymerase chain reaction. Our results revealed that miR-21-3p is significantly overexpressed in the OSCC tissues compared with the corresponding adjacent normal tissues (p<0.001). High miR-21-3p expression levels were significantly correlated with N classification (p=0.042). After transfection with a miR-21-3p inhibitor (antagomir), the invasive ability of the OSCC cells was significantly abrogated. Altogether, our findings indicated that miR-21-3p plays a crucial oncogenic role in cell metastasis during OSCC progression.
Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Department of anatomic pathology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taipei, Taiwan, R.O.C.
Hui-Hwa
HH
Tseng
Department of Orthopedics, Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C.|Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan, R.O.C.
Yu-Kai
YK
Tseng
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, R.O.C.
Jyun-Jie
JJ
You
Department of Otorhinolaryngology-Head & Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei, Taiwan, R.O.C.
Bor-Hwang
BH
Kang
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, R.O.C.
Tsung-Han
TH
Wang
Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
Cheng-Mei
CM
Yang
Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
Hung-Chih
HC
Chen
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.
Huei-Han
HH
Liou
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan, R.O.C.
Pei-Feng
PF
Liu
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, R.O.C.
Luo-Ping
LP
Ger
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C. kwtsai@vghks.gov.tw.|Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan, R.O.C.
Kuo-Wang
KW
Tsai
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D060888:Real-Time Polymerase Chain Reaction; D014945:Wound Healing
C524940:MIRN21 microRNA, human; D035683:MicroRNAs
miR-21; microRNA; next-generation sequencing; oral cancer