Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.
G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. It has been shown that the 3' G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures, and the human telomeric DNA G-quadruplexes are considered attractive targets for anticancer drugs. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR and biophysical methodology used in our research group for the study of G-quadruplex structures in physiologically relevant solution and their interactions with small-molecule compounds.
Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, 575 Stadium Mall Dr., West Lafayette, IN, 47907, USA.
Clement
C
Lin
Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, 575 Stadium Mall Dr., West Lafayette, IN, 47907, USA. yangdz@purdue.edu.|Purdue Center for Cancer Research, Purdue University, 201 S. University St., West Lafayette, IN, 47907, USA. yangdz@purdue.edu.|Purdue Institute for Drug Discovery, Purdue University, 720 Clinic Dr., West Lafayette, IN, 47907, USA. yangdz@purdue.edu.
Danzhou
D
Yang
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D019098:Telomerase / Q000737:chemistry*; D016615:Telomere / Q000737:chemistry*
D006147:Guanine; D004247:DNA; D019098:Telomerase
Anticancer drug targets; G-Quadruplex structures; G-Quadruplex-interactive compounds; Human telomeres; Structure polymorphism; Telomerase inhibitors