Optimization of cellulase production by Penicillium sp.
The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp. Yields of Fpase, CMCase and β-glucosidase, attained on optimized medium with Penicillium sp. were 8.7, 25 and 9.52 U/ml, respectively with increment of 9.2, 5.9 and 43.8-folds over titers of the respective enzyme on unoptimised medium. Cellulase of the fungal culture with the ratio of β-glucosidase to Fpase greater than one will hold potential for biotechnological applications.
Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India.
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Prasanna
Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India.
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Ramanjaneyulu
Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India. rajasekharb64@gmail.com.
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http://orcid.org/0000-0001-6665-3627
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Rajasekhar Reddy
Endoglucanase; Exoglucanase; Nutrients; Optimization; Penicillium sp.; β-Glucosidase