Acute toxicity, bioaccumulation and effects of dietary transfer of silver from brine shrimp exposed to PVP/PEI-coated silver nanoparticles to zebrafish.
The extensive use and release to the aquatic environment of silver nanoparticles (NPs) could lead to their incorporation into the food web. Brine shrimp larvae of 24h showed low sensitivity to the exposure to PVP/PEI-coated Ag NPs (5nm), with EC50values at 24h of 19.63mgAgL-1, but they significantly accumulated silver after 24h of exposure to 100μgL-1of Ag NPs. Thus, to assess bioaccumulation and effects of silver transferred by the diet in zebrafish, brine shrimp larvae were exposed to 100ngL-1of Ag NPs as an environmentally relevant concentration or to 100μgL-1as a potentially effective concentration and used to feed zebrafish for 21days. Autometallography revealed a dose- and time-dependent metal accumulation in the intestine and in the liver of zebrafish. Three-day feeding with brine shrimps exposed to 100ngL-1of Ag NPs was enough to impair fish health as reflected by the significant reduction of lysosomal membrane stability and the presence of vacuolization and necrosis in the liver. However, dietary exposure to 100μgL-1of Ag NPs for 3days did not significantly alter gene transcription levels, neither in the liver nor in the intestine. After 21days, biological processes such as lipid transport and localization, cellular response to chemical stimulus and response to xenobiotic stimulus were significantly altered in the liver. Overall, these results indicate an effective dietary transfer of silver and point out to liver as the main target organ for Ag NP toxicity in zebrafish after dietary exposure.
CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
José María
JM
Lacave
CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
Álvaro
Á
Fanjul
CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
Eider
E
Bilbao
Dept. of Applied Mathematics, Statistics and Operations Research, Science and Technology Faculty, and Basque Center for Applied Mathematics - BCAM, University of the Basque Country (UPV/EHU), Leioa, Spain.
Nerea
N
Gutierrez
Dept. of Applied Mathematics, Statistics and Operations Research, Science and Technology Faculty, and Basque Center for Applied Mathematics - BCAM, University of the Basque Country (UPV/EHU), Leioa, Spain.
Irantzu
I
Barrio
Dept. of Applied Mathematics, Statistics and Operations Research, Science and Technology Faculty, and Basque Center for Applied Mathematics - BCAM, University of the Basque Country (UPV/EHU), Leioa, Spain.
Inmaculada
I
Arostegui
CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
Miren P
MP
Cajaraville
CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain. Electronic address: amaia.orbea@ehu.eus.
Amaia
A
Orbea
D000818:Animals; D001156:Artemia / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005506:Food Contamination; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D007814:Larva / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007928:Lethal Dose 50; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D008247:Lysosomes / Q000187:drug effects / Q000473:pathology; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009336:Necrosis; D011094:Polyethyleneimine / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D011205:Povidone / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D012834:Silver / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D013499:Surface Properties; D014018:Tissue Distribution; D023382:Toxicity Tests, Acute; D066007:Toxicokinetics; D014617:Vacuoles / Q000187:drug effects / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development / Q000378:metabolism*
D014874:Water Pollutants, Chemical; D012834:Silver; D011094:Polyethyleneimine; D011205:Povidone
Bioaccumulation; Brine shrimp; Cellular effects; Dietary transfer; Silver nanoparticles; Zebrafish