Boosting the growth of the probiotic strain Lactobacillus paracasei ssp. paracasei F19.
Single so-called booster substances were added to the fermentation medium of the probiotic strain Lactobacillus (L.) paracasei ssp. paracasei F19 to enhance its growth. A wide screening was carried out in microtiter plates and a statistical analysis of the growth parameters was performed. CFU counts were used to correlate the increase in OD590nmwith the increase in viable cell number. Sodium ascorbate, sodium pyruvate, manganese sulfate and cysteine had a remarkable boosting effect on the growth of L. paracasei F19. Three of the boosters increased the growth rate of the strain and led to a higher cell density and biomass yield in laboratory conditions. Cysteine significantly shortened the lag phase, therefore reducing the fermentation times. The boosters were tested on four additional Lactobacillus species and their growth boosting activity was retained. To investigate whether the growth boosters could improve the tolerance of L. paracasei F19 to the adverse condition in the GI tract, additional tests were performed. Sodium ascorbate and sodium pyruvate exerted a certain antioxidant effect, as they improved the tolerance of L. paracasei F19 to H2O2. Sodium ascorbate enhanced the growth of the strain in low pH.
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany.
Desideria
D
Brignone
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany.
Pia
P
Radmann
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany. juergen.behr@wzw.tum.de.|Bavarian Center for Biomolecular Mass Spectrometry, Technische Universität München, Gregor-Mendel Str. 4, 85354, Freising, Germany. juergen.behr@wzw.tum.de.
Jürgen
J
Behr
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany.
Rudi F
RF
Vogel
D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D000069980:Lacticaseibacillus paracasei / Q000254:growth & development* / Q000378:metabolism; D019936:Probiotics / Q000032:analysis / Q000378:metabolism*
D003470:Culture Media
Biomass; Fitness; Growth booster; Lactobacillus paracasei ssp. paracasei F19; Probiotics