EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ONESCHERICHIA COLI.
BACKGROUND
Escherichia coliis the agent responsible for a range of clinical diseases. With emerging antimicrobial resistance, other treatment options including solar/photo-therapy are becoming increasingly common. Visible Range Radiation Therapy/Colour Therapy is an emerging technique in the field of energy/vibrational medicine that uses visible spectrum of Electromagnetic Radiations to cure different diseases. In this study, our goal was to understand the effect of Visible Range Electromagnetic Radiations on E. coli (in vitro) and therefore find out the most appropriate visible range radiation for the treatment of diseases caused by E. coli.


METHODS
A total of 6 non-repetitive E. coli isolates were obtained from urine samples obtained from hospitalized patients with UTI. Single colony of E. coli was inoculated in 3 ml of Lysogeny Broth (LB) and 40 μl of this E. coli suspension was poured into each of the plastic tubes which were then irradiated with six different wavelengths in the visible region (Table. 1) after 18 hours with one acting as a control. The Optical Densities of these irradiated samples were then measured. Furthermore, scanning electron microscopy (TEFCAN ZEGA3) was carried out.


RESULTS
The analysis of the microscopic and SEM images of irradiated E. coli samples with six different visible range radiations is representative of The fact that E. coli responded differently to every applied radiation in the visible region and the most profound inhibitory effects were that of 538nm Visible Range Radiation (Green) which proved to be bactericidal and 590nm Visible Range Radiation (yellow) which was bacteriostatic. The enhanced growth of E. coli with varying degrees was clearly observed in 610nm (orange), 644nm (red), 464nm (Purple) and 453nm (blue).


CONCLUSIONS
It can be concluded that 538nm (Green) and 590nm (Yellow) can effectively be used for treating E. coli borne diseases.
Address: COMSATS University, Lahore, Pakistan.
Samina T Yousuf
ST
Azeemi
Address: COMSATS University, Lahore, Pakistan.
Saleem Farooq
SF
Shaukat
Khanwada Silsila e Azeemia, Markazi Muraqba Hall, Sector 4-C, Surjani Town, Karachi, Pakistan.
Khawaja Shamsuddin
KS
Azeemi
Centre for Applied Molecular Biology (CAMB), Lahore, Pakistan.
Idrees
I
Khan
University of Lahore, Lahore, Pakistan.
Khalid
K
Mahmood
Centre for Excellence in Molecular Biology (CEMB), Lahore, Pakistan.
Farah
F
Naz
D004574:Electromagnetic Fields; D004926:Escherichia coli / Q000254:growth & development / Q000528:radiation effects*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D008027:Light
Alternative Treatment; Colour Therapy; E. coli; UTI; Visible Range Radiation Therapy