Comparison of different methods for extraction of Cinnamomi ramulus: yield, chemical composition and in vitro antiviral activities.
Hydrodistillation (HD), supercritical fluid extraction (SFE) and reflux extraction (RE) were applied to obtain Cinnamomi ramulus extracts. The yields, chemical compositions and antiviral activities of the extracts were investigated. Extracts were analysed using gas chromatography-mass spectrometry and the antiviral activities were evaluated using cytopathic effect inhibition assay. HD, SFE and RE afforded 0.376, 1.227 and 5.914% yields, respectively. Cinnamaldehyde (CA), SFE and ethanol extracts exhibited antiviral activities against herpes simplex virus type 1. Moreover, CA and other three extracts had inhibition efficacy against respiratory syncytial virus. The most efficient antiviral activities were obtained with SFE.
a Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.|b Technology Transfer Center , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
Jing
J
Zhou
b Technology Transfer Center , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
Xiurong
X
Yuan
a Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
Ling
L
Li
a Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.|c School of Pharmacy , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
Tong
T
Zhang
a Experiment Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.|c School of Pharmacy , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
Bing
B
Wang
D000171:Acrolein / Q000031:analogs & derivatives; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D025924:Chromatography, Supercritical Fluid / Q000379:methods* / Q000592:standards; D057168:Distillation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D018259:Herpesvirus 1, Human / Q000187:drug effects; D008722:Methods; D009822:Oils, Volatile / Q000737:chemistry*; D010936:Plant Extracts; D012136:Respiratory Syncytial Viruses / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D010936:Plant Extracts; D000171:Acrolein; C012843:cinnamaldehyde
Cinnamaldehyde; Cinnamomi ramulus; GC–MS; antiviral activities; ethanol extraction; hydrodistillation; supercritical fluid extraction