Endovenous Laser Ablation of Varicose Veins Preserves Biological Properties of Vascular Endothelium and Modulates Proinflammatory Agent Profile More Favorably Than Classic Vein Stripping.
Here we compared effect of serum from varicose patients undergoing endovenous laser ablation (EVLA) and classic vein stripping (CVS) on biological properties of endothelial cells and on the local and systemic profiles of proinflammatory agents. Results showed that serum from EVLA patients improved proliferation and reduced senescence and oxidative stress in the endothelial cells, as compared with the serum from CVS patients. These effects were related to a suppressed activity of TGF-β1, the level of which in the serum from the EVLA patients was decreased. Medium generated by the cells subjected to EVLA serum contained decreased amounts of ICAM-1, VCAM-1, and E-selectin and increased amount of uPA, whereas the serum itself contained decreased concentrations of ICAM-1, E-selectin, and P-selectin and increased concentrations of uPA, PAI-1, and TFPI. Both EVLA and CVS resulted in diversified patients' reaction with respect to a direction of postprocedure changes in proinflammatory factors' serum level. Analysis of proportions showed that the groups differed remarkably in case of ICAM-1 and ET-1, the level of which declined in a higher fraction of patients treated endovenously. Our findings indicate that EVLA preserves better than CVS the functionality of vascular endothelium and modulates better both local and systemic profile of proinflammatory mediators.
Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str., 61-848 Poznań, Poland.
Paweł
P
Uruski
Department of General and Vascular Surgery, Poznań University of Medical Sciences, Długa 1/2 Str., 61-848 Poznań, Poland.
Krzysztof
K
Aniukiewicz
Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str., 61-848 Poznań, Poland.
Justyna
J
Mikuła-Pietrasik
Department of Pathophysiology, Poznań University of Medical Sciences, Rokietnicka 8 Str., 60-806 Poznań, Poland.
Patrycja
P
Sosińska
Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str., 61-848 Poznań, Poland.
Andrzej
A
Tykarski
Department of Hypertensiology, Angiology and Internal Medicine, Poznań University of Medical Sciences, Długa 1/2 Str., 61-848 Poznań, Poland.
Krzysztof
0000-0002-6066-5747
K
Książek
Department of General and Vascular Surgery, Poznań University of Medical Sciences, Długa 1/2 Str., 61-848 Poznań, Poland.
Zbigniew
Z
Krasiński
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D016922:Cellular Senescence; D019040:E-Selectin / Q000378:metabolism; D004730:Endothelium, Vascular / Q000473:pathology*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D019007:P-Selectin / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014648:Varicose Veins / Q000601:surgery*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D014656:Vascular Surgical Procedures
D019040:E-Selectin; C573420:ICAM1 protein, human; D019007:P-Selectin; C546936:SELE protein, human; C546937:SELP protein, human; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1