Identification, purification, and expression patterns of chitinase from psychrotolerant Pedobacter sp. PR-M6 and antifungal activity in vitro.
In this study, a novel psychrotolerant chitinolytic bacterium Pedobacter sp. PR-M6 that displayed strong chitinolytic activity on 0.5% colloidal chitin was isolated from the soil of a decayed mushroom. Chitinase activity of PR-M6 at 25 °C (C25) after 6 days of incubation with colloidal chitin increased rapidly to a maximum level (31.3 U/mg proteins). Three chitinase isozymes (chiII, chiIII, and chiIV) from the crude enzyme at 25 °C (C25) incubation were expressed on SDS-PAGE gels at 25 °C. After purification by chitin-affinity chromatography, six chitinase isozymes (chiI, chiII, chiIII, chiIV, chiV, and chiVI) from C25-fractions were expressed on SDS-PAGE gels at 25 °C. Major bands of chitinase isozymes (chiI, chiII, and chiIII) from C4-fractions were strongly expressed on SDS-PAGE gels at 25 °C. Pedobacter sp. PR-M6 showed high inhibition rate of 60.9% and 57.5% against Rhizoctonia solani and Botrytis cinerea, respectively. These results indicated that psychrotolerant Pedobacter sp. PR-M6 could be applied widely as a microorganism agent for the biocontrol of agricultural phytopathogens at low temperatures.
Department of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea.
Yong-Su
YS
Song
Department of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea.
Dong-Jun
DJ
Seo
Department of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 61186, Republic of Korea. Electronic address: woojung@jnu.ac.kr.
Woo-Jin
WJ
Jung
D000383:Agriculture; D000935:Antifungal Agents / Q000302:isolation & purification*; D061046:Biological Control Agents / Q000302:isolation & purification; D020171:Botrytis / Q000187:drug effects; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D057075:Enzyme Assays; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D060485:Pedobacter / Q000145:classification / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D010802:Phylogeny; D012232:Rhizoctonia / Q000187:drug effects; D012988:Soil Microbiology
D000935:Antifungal Agents; D061046:Biological Control Agents; D007527:Isoenzymes; D002686:Chitin; D002688:Chitinases
Chitinase isozyme; Chitinolytic activity; Pedobacter sp.; Psychrotolerance