Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation.
Rapid freeze-freeze substitution after glutaraldehyde fixation (CF-FS method) obtained the natural and fine structures of macrophages and engulfed yeast cells. Culturing macrophages on single hole molybdenum grids placed in culture dishes made possible the rapid freezing of cells by the 'open sandwich method'. This method may be convenient when rapid-freezing cannot be performed immediately, or when a rapid-freezing device is not available in the lab.
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku Chiba 260-8673,Japan.
Masashi
M
Yamaguchi
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku Chiba 260-8673,Japan.
Azusa
A
Takahashi-Nakaguchi
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku Chiba 260-8673,Japan.
Yuko
Y
Aida
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku Chiba 260-8673,Japan.
Michiyo
M
Sato-Okamoto
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku Chiba 260-8673,Japan.
Hiroji
H
Chibana
D000818:Animals; D002175:Candida / Q000648:ultrastructure*; D002460:Cell Line; D015925:Cryopreservation / Q000379:methods*; D005404:Fixatives; D017110:Freeze Substitution / Q000379:methods*; D005976:Glutaral; D008264:Macrophages / Q000166:cytology* / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010587:Phagocytosis; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D016707:Tissue Fixation / Q000379:methods*
D005404:Fixatives; D005976:Glutaral
cultured macrophages; freeze-substitution; molybdenum grids; open sandwich method; ultrastructure; yeast cells