MiR-15a suppresses hepatocarcinoma cell migration and invasion by directly targetingcMyb.
OBJECTIVE
This study aimed to determine the function of miR-15a in HCC, and identifycMybas a target of miR-15a.


METHODS
RNA expression was evaluated by quantitative real-time PCR (qRT-PCR). The effects of miR-15a orcMybon HCC cells were evaluated by transwell migration assay and western blot analysis.CMyb, the predicted target, has been frequently verified by luciferase assay.


RESULTS
MiR-15a was markedly downregulated in sphere culture HCC cells by qRT-PCR.CMybwas predicted to be a potential target of miR-15a using bioinformatics analysis. This prediction has been frequently verified by luciferase assay and western blot. A positive correlation betweencMyband the migration ability of HCC cells was demonstrated by transwell assays. MiR-15a mimic suppressedcMybexpression to weaken HCC cell migration ability. On the other hand, miR-15a inhibitor upregulatedcMyband induced HCC cell migration.


CONCLUSIONS
MiR-15a could suppress HCC progression through the repression ofcMyb, making miR-15a a potential therapeutic target.
Oncology Center, Zhujiang Hospital of Southern Medical University Guangzhou 510282, Guangdong, China.
Bo
B
Liu
Oncology Center, Zhujiang Hospital of Southern Medical University Guangzhou 510282, Guangdong, China.
Ting
T
Sun
Oncology Center, Zhujiang Hospital of Southern Medical University Guangzhou 510282, Guangdong, China.
Gang
G
Wu
Department of Oncology, Foshan Hospital, Southern Medical University Foshan 528000, Guangdong, China.
Hui
H
Shang-Guan
Oncology Center, Zhujiang Hospital of Southern Medical University Guangzhou 510282, Guangdong, China.
Zhao-Jin
ZJ
Jiang
Oncology Center, Zhujiang Hospital of Southern Medical University Guangzhou 510282, Guangdong, China.
Ji-Ren
JR
Zhang
Oncology Center, Zhujiang Hospital of Southern Medical University Guangzhou 510282, Guangdong, China.
Yan-Fang
YF
Zheng
Hepatocarcinoma; MiR-15a; cMyb