Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum.
The unicellular green alga, Penium margaritaceum, represents a novel and valuable model organism for elucidating cell wall dynamics in plants. This organism's cell wall contains several polymers that are highly similar to those found in the primary cell walls of land plants. Penium is easily grown in laboratory culture and is effectively manipulated in various experimental protocols including microplate assays and correlative microscopy. Most importantly, Penium can be live labeled with cell wall-specific antibodies or other probes and returned to culture where specific cell wall developmental events can be monitored. Additionally, live cells can be rapidly cryo-fixed and cell wall surface microarchitecture can be observed with variable pressure scanning electron microscopy. Here, we describe the methodology for maintaining Penium for experimental cell wall enzyme studies.
Department of Biology, Skidmore College, Saratoga Springs, NY, 12866, USA. ddomoz@skidmore.edu.
David
D
Domozych
Department of Biology, Skidmore College, Saratoga Springs, NY, 12866, USA.
Anna
A
Lietz
Department of Biology, Skidmore College, Saratoga Springs, NY, 12866, USA.
Molly
M
Patten
Department of Biology, Skidmore College, Saratoga Springs, NY, 12866, USA.
Emily
E
Singer
Department of Biology, Skidmore College, Saratoga Springs, NY, 12866, USA.
Berke
B
Tinaz
Department of Biology, Skidmore College, Saratoga Springs, NY, 12866, USA.
Sandra C
SC
Raimundo
D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D000460:Chlorophyta / Q000378:metabolism* / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D008855:Microscopy, Electron, Scanning; D057054:Molecular Imaging / Q000379:methods*; D059828:Plant Cells / Q000378:metabolism; D011108:Polymers / Q000378:metabolism
D011108:Polymers
CLSM; Cell wall; Enzymes; Live-cell immunolabeling; Monoclonal antibodies; Penium margaritaceum; VP-SEM