Overexpression of Hsa-miR-320 Is Associated With Invasion and Metastasis of Ovarian Cancer.
Ovarian cancer is one of the most common malignant tumor of female genital organs which ranks the third morbidity. We aimed to provide a better understanding of the mechanism of invasion and metastasis of ovarian cancer. The ovarian cancer samples were downloaded from GEO. Then clustering was performed to classify the stage of miRNAs based on the difference of prognosis and metastasis. Furthermore, the miRNAs model was build and the survival analysis processes was performed to observe the influence on prognosis, invasion and metastasis. At last, miRNAs co-expression network was built to explore the core miRNAs and the risk classification model was built to perform the risk assessment based on these core miRNAs. A total of 17 significantly differential expressed miRNAs were obtained. Functional enrichment of 1,488 target genes, pathways like cell cycle, focal adhesion, and pathways in cancer, which are closely related to the proliferation and metastasis of cancer cells were highly enriched, this indicate that these miRNAs are related to the proliferation and metastasis of cancer cells. The co-expressed network shows that the high expression of hsa-miR-320 indicated negative prognosis and high risk of metastasis. In conclusion, the expression level of hsa-miR-320 is highly related to the migration and invasion of cancer. The high expression of hsa-miR-320 directly indicated negative prognosis and high risk of metastasis. These findings reveal that hsa-miR-320 may serve as an important therapeutic target in ovarian cancer therapy. J. Cell. Biochem. 118: 3654-3661, 2017. © 2017 Wiley Periodicals, Inc.
Reproductive Center, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, China.
Wei
W
Wang
Department of Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Juan
J
Yang
Cervical Disease Clinic, Jiangsu Huai'an Maternity and Children Hospital, Huai'an, China.
Yuan-Yuan
YY
Xiang
Reproductive Center, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, China.
Jie
0000-0001-9325-6555
J
Pi
Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated Six People's Hospital, 600 Yishan Road, Shanghai, 200233, China.
Jiang
J
Bian
D030541:Databases, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D012307:Risk Factors
C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm
CO-REGULATION NETWORK; Hsa-miR-320; KAPLAN-MEIER; OVARIAN CANCER; miRNAs