DJ-1, a Parkinson's disease related protein, aggregates under denaturing conditions and co-aggregates with α-synuclein through hydrophobic interaction.
DJ-1, a small ubiquitously expressed protein implicated in several pathways associated with Parkinson's disease pathogenesis, has been found to interact with α-synuclein and modulate its aggregation, yet the exact mechanisms remain unclear.



The stability and aggregation properties of wild-type DJ-1 under denaturing conditions, such as low pH, high temperature, presence of denaturants were investigated. The interaction between DJ-1 and α-synuclein was tested by SDS-PAGE gel and native gel electrophoresis and by size-exclusion HPLC. Fibrillization was monitored by thioflavin T fluorescence assays and amorphous aggregation was followed by light scattering measurements. The morphology of aggregated species was observed by transmission electron microscopy and atomic force microscopy. Protein secondary structures were characterized by far-UV circular dichroism.



DJ-1 fibrillization was first observed at low pH or by adding denaturants. Amorphous aggregates formed at neutral pH, and the aggregation was dramatically accelerated by elevated temperature and the presence of α-synuclein. Aggregation of DJ-1 were enhanced by heating and perturbed by the co-occurrence of α-synuclein but strong interactions between the two proteins were not found.



Varying environmental factors led to different aggregation pathways of DJ-1 although a simulated physiological condition would not lead to fibrillization. DJ-1 co-aggregating with α-synuclein may result from weak hydrophobic interaction and DJ-1 exhibited chaperon-like activity in the initial time of α-synuclein aggregation at high temperature.



This research on DJ-1 presented its aggregation behavior under denaturing conditions and interaction mechanism with α-synuclein that may help to decipher its potential neuroprotective or neurotoxic role in Parkinson's disease.
Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064,USA.
Min
M
Zhu
Department of Chemistry and Physics, Fayetteville State University, Fayetteville, NC 28301, USA.
Shivaliben
S
H Patel
Department of Chemistry and Physics, Fayetteville State University, Fayetteville, NC 28301, USA. Electronic address: shan@uncfsu.edu.
Shubo
S
Han
D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D010300:Parkinson Disease / Q000209:etiology; D066329:Protein Aggregates; D011487:Protein Conformation; D000071617:Protein Deglycase DJ-1 / Q000737:chemistry*; D011489:Protein Denaturation; D013696:Temperature; D051844:alpha-Synuclein / Q000737:chemistry*
D066329:Protein Aggregates; D051844:alpha-Synuclein; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1
Aggregation; DJ-1; Denaturing conditions; Fibrillization; Parkinson's disease; α-Synuclein