Mesophilic continuous fermentative hydrogen production from acid pretreated de-oiled jatropha waste hydrolysate using immobilized microorganisms.
Mesophilic hydrogen production from acid pretreated hydrolysate (biomass concentration of 100g/L and 2% hydrochloric acid) of de-oiled jatropha waste was carried out in continuous system using immobilized microorganisms at various hydraulic retention times (HRTs) ranging from 48 to 12h. The experimental results of the reusability of immobilized microorganisms showed their stability up to 10 cycles with an average cumulative hydrogen production of 770mL/L. The peak hydrogen production rate and hydrogen yield were 0.9L/L*d and 86mL/greducing sugars added, respectively at 16h HRT, with butyrate as the predominant volatile fatty acid. The microbial community analysis revealed that majority of the PCR-DGGE bands were assigned to genus Clostridium and were perhaps the key drivers of the higher hydrogen production.
Sustainable Management of Natural Resources and Environment Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Vietnam. Electronic address: gopalakrishnankumar@tdt.edu.vn.
Gopalakrishnan
G
Kumar
Center for Materials Cycles and Waste Management Research, National Institute for Environmental Studies, Tsukuba, Japan.
Periyasamy
P
Sivagurunathan
Center for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
Biswarup
B
Sen
Department of Environmental Engineering, Daegu University, Gyeongsan, Republic of Korea.
Sang-Hyoun
SH
Kim
Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan.
Chiu-Yue
CY
Lin
D000143:Acids; D019149:Bioreactors; D005232:Fatty Acids, Volatile; D006859:Hydrogen; D031286:Jatropha
D000143:Acids; D005232:Fatty Acids, Volatile; D006859:Hydrogen
Acid pretreated hydrolysate; Deoiled jatropha waste; Immobilization; PCR-DGGE; Soluble metabolic products