Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles.
Hierarchical copper shells anchored on magnetic nanoparticles were designed and fabricated to selectively deplete hemoglobin from human blood by immobilized metal affinity chromatography. Briefly, CoFe2O4nanoparticles coated with polyacrylic acid were first synthesized by a one-pot solvothermal method. Hierarchical copper shells were then deposited by immobilizing Cu2+on nanoparticles and subsequently by reducing between the solid CoFe2O4@COOH and copper solution with NaBH4. The resulting nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. The particles were also tested against purified bovine hemoglobin over a range of pH, contact time, and initial protein concentration. Hemoglobin adsorption followed pseudo-second-order kinetics and reached equilibrium in 90 min. Isothermal data also fit the Langmuir model well, with calculated maximum adsorption capacity 666 mg g-1. Due to the high density of Cu2+on the shell, the nanoparticles efficiently and selectively deplete hemoglobin from human blood. Taken together, the results demonstrate that the particles with hierarchical copper shells effectively remove abundant, histidine-rich proteins, such as hemoglobin from human blood, and thereby minimize interference in diagnostic and other assays.
School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China; Henan Collaborative Innovation Center of Molecular Diagnostics and Laboratory Medicine, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
Youxun
0000-0001-9852-247X
Y
Liu
School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
Yaokun
Y
Wang
School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
Mingyang
M
Yan
School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
Juan
0000-0003-2546-9166
J
Huang
D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis* / Q000302:isolation & purification*; D006639:Histidine / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D060306:Magnetometry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011506:Proteins / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D000099053:Histidine-Rich Glycoprotein
D000180:Acrylic Resins; D006454:Hemoglobins; D058185:Magnetite Nanoparticles; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein; C006903:carbopol 940; D006639:Histidine; D003300:Copper