HSP90 and pCREB alterations are linked to mancozeb-dependent behavioral and neurodegenerative effects in a marine teleost.
The pesticide mancozeb (mz) is recognized as a potent inducer of oxidative stress due to its ability to catalyze the production of reactive oxygen species plus inhibiting mitochondrial respiration thus becoming an environmental risk for neurodegenerative diseases. Despite numerous toxicological studies on mz have been directed to mammals, attention on marine fish is still lacking. Thus, it was our intention to evaluate neurobehavioral activities of ornate wrasses (Thalassoma pavo) exposed to 0.2mg/l of mz after a preliminary screening test (0.07-0.3mg/l). Treated fish exhibited an evident (p<0.001) latency to reach T-maze arms (>1000%) while exploratory attitudes (total arm entries) diminished (-50%; p<0.05) versus controls during spontaneous exploration tests. Moreover, they showed evident enhancements (+111%) of immobility in the cylinder test. Contextually, strong (-88%; p<0.01) reductions of permanence in light zone of the Light/Dark apparatus along with diminished crossings (-65%) were also detected. Conversely, wrasses displayed evident enhancements (160%) of risk assessment consisting of fast entries in the dark side of this apparatus. From a molecular point of view, a notable activation (p<0.005) of the brain transcription factor pCREB occurred during mz-exposure. Similarly, in situ hybridization supplied increased HSP90 mRNAs in most brain areas such as the lateral part of the dorsal telencephalon (Dl; +68%) and valvula of the cerebellum (VCe; +35%) that also revealed evident argyrophilic signals. Overall, these first indications suggest a possible protective role of the early biomarkers pCREB and HSP90 against fish toxicity.
Comparative Neuroanatomy Laboratory, Biology, Ecology and Earth Science Department (DiBEST), University of Calabria, Arcavacata of Rende, 87036, CS, Italy.
Merylin
M
Zizza
Department of Biology, Section of Evolutionary and Comparative Biology, University of Naples Federico II, 80134 Naples, Italy.
Mariana
M
Di Lorenzo
Department of Biology, Section of Evolutionary and Comparative Biology, University of Naples Federico II, 80134 Naples, Italy.
Vincenza
V
Laforgia
Department of Chemistry and Chemical Technologies (DCTC), University of Calabria, Arcavacata of Rende, 87036, CS, Italy.
Emilia
E
Furia
Department of Chemistry and Chemical Technologies (DCTC), University of Calabria, Arcavacata of Rende, 87036, CS, Italy.
Giovanni
G
Sindona
Comparative Neuroanatomy Laboratory, Biology, Ecology and Earth Science Department (DiBEST), University of Calabria, Arcavacata of Rende, 87036, CS, Italy.
Marcello
M
Canonaco
Comparative Neuroanatomy Laboratory, Biology, Ecology and Earth Science Department (DiBEST), University of Calabria, Arcavacata of Rende, 87036, CS, Italy. Electronic address: rm.facciolo@unical.it.
Rosa Maria
RM
Facciolo
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005399:Fishes / Q000235:genetics / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000633:toxicity*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008344:Maneb / Q000633:toxicity*; D009043:Motor Activity / Q000187:drug effects; D009410:Nerve Degeneration; D020258:Neurotoxicity Syndromes / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000523:psychology; D011930:Reaction Time / Q000187:drug effects; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015038:Zineb / Q000633:toxicity*
D017362:Cyclic AMP Response Element-Binding Protein; D029941:Fish Proteins; D005659:Fungicides, Industrial; D018841:HSP90 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D008344:Maneb; C013099:mancozeb; D015038:Zineb
Behavioral impairments; HSP90 mRNA; Manganese/zinc-ethylene-bis-dithiocarbamate; Neuronal damages; Phosphorylated CREB factor