The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid-Loaded Dendritic Cells.
Cell-based assays are widely used in all aspects of research ranging from understanding basic biological function to identifying compounds for disease intervention. Immortalized cell lines have been ideal components of these assays due to the low cost of growth, easy maintenance, and the ability to obtain homogenous cell populations. Like other molecular assays, cell-based systems have been automated to reduce experimental error. Complex lipids are now recognized as important components of the allergic response, the study of the interaction between NKTs and lipid-activated DCs opens a new perspective into the intrinsic allergenicity of proteins. Here, we describe a semi-automated method to measure IL-2 release upon activation of mouse NKT cells (DN32.D3) by various lipids in a 384-well plate using the Biomek®3000 laboratory automated workstation (Beckman Coulter).
School of Biosciences, University of Nottingham, College Road, Sutton Bonington Campus, Sutton Bonington, Loughborough, LE12 5RD, UK. ashfaqghumra@hotmail.com.
Ashfaq
A
Ghumra
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, Nottingham, UK.
Marcos
M
Alcocer
D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D008055:Lipids / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D055611:Natural Killer T-Cells / Q000276:immunology*
D007376:Interleukin-2; D008055:Lipids
Biomek® 3000; Cell-based assays; ELISA; Natural Killer T (NKT) cells; Robotics