Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs.
Lipophilicity is one of the essential properties influencing drug absorption, excretion and metabolism. It is used for screening viable drug candidates. Chromatographic behavior of thiepino[3,2-c:6,7-c']dipyridine and 16 benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs was studied using thin-layer chromatography under typical reversed-phase conditions and two microemulsion chromatographic systems. Seventeen chromatographic and nine in silico lipophilicity measures were estimated. They were compared by classical multivariate approaches: principal component analysis, hierarchical cluster analysis, and ranked and grouped by the non-parametric method-Sum of ranking differences. Two computational and two chromatographic descriptors from the typical reversed-phase conditions using acetone/water mixtures emerged as the best candidates for lipophilicity estimation. The principal component scores related to typical reversed-phase conditions using dioxane/water were ranked as statistically insignificant (the worst). Microemulsion systems were positioned in between, performing worse than in silico estimates. Thiepine derivatives were ranked and grouped by sum of ranking differences, fusing multiple lipophilicity measures. In multicriteria maximization ranking, the compound substituted by phenyl group at position 8 was selected as the most lipophilic one. It is also the most active against Candida albicans. The ranking confirmed that introduction of phenyl core is essential for increasing the lipophilicity of the studied compounds.
Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
Sandra
S
Šegan
Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Nina
N
Božinović
Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Igor
I
Opsenica
Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Filip
F
Andrić
D000935:Antifungal Agents / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D016000:Cluster Analysis; D008055:Lipids / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*
D000935:Antifungal Agents; D008055:Lipids; D011725:Pyridines
benzothiepino[3,2-c]pyridines; lipophilicity; multicriteria ranking; multivariate data analysis; sum of ranking differences