Control of dental-derived induced pluripotent stem cells through modified surfaces for dental application.
OBJECTIVE
The aim of this study is to investigate the behaviour of iPSc derived from dental stem cells in terms of initial adhesion, differentiation potential on differently surface-treated titanium disc.


METHODS
iPSc derived from human gingival fibroblasts (hGFs) were established using 4-reprogramming factors transduction with Sendai virus. The hGF-iPSc established in this study exhibited the morphology and growth properties similar to human embryonic stem (ES) cells and expressed pluripotency makers. Alkaline Phosphatase (AP) staining, Embryoid Body (EB) formation and in vitro differentiation and karyotyping further confirmed pluripotency of hGF-iPSc. Then, hGF-iPSc were cultured on machined- and Sandblasted and acid etched (SLA)-treated titanium discs with osteogenic induction medium and their morphological as well as quantitative changes according to different surface types were investigated using Alizrin Red S staining, Scanning electron microscopy (SEM), Flow cytometry and RT-PCR.


RESULTS
Time-dependent and surface-dependent morphological changes as well as quantitative change in osteogenic differentiation of hGF-iPSc were identified and osteogenic gene expression of hGF-iPSc cultured on SLA-treated titanium disc found to be greater than machined titanium disc, suggesting the fate of hGF-iPSc may be determined by the characteristics of surface to which hGF-iPSc first adhere.


CONCLUSIONS
iPSc derived from dental stem cell can be one of the most promising and practical cell sources for personalized regenerative dentistry and their morphological change as well as quantitative change in osteogenic differentiation according to different surface types may be further utilized for future clinical application incorporated with dental implant.
a Department of Prosthodontics, Oral Science Research Center, BK21 Plus Project , Yonsei University College of Dentistry , Seoul , Korea.
Hyunmin
H
Choi
a Department of Prosthodontics, Oral Science Research Center, BK21 Plus Project , Yonsei University College of Dentistry , Seoul , Korea.
Kyu-Hyung
KH
Park
a Department of Prosthodontics, Oral Science Research Center, BK21 Plus Project , Yonsei University College of Dentistry , Seoul , Korea.
Ah-Reum
AR
Lee
b Division of Rheumatology, Department of Internal Medicine , Institute for Immunology and Immunological Disease, Yonsei University College of Medicine , Seoul , Korea.|c Brain Korea 21 Project for Medical Science , Yonsei University , Seoul , Korea.|d Severance Biomedical Institute, Yonsei University College of Medicine , Seoul, Korea.
Chin Hee
CH
Mun
b Division of Rheumatology, Department of Internal Medicine , Institute for Immunology and Immunological Disease, Yonsei University College of Medicine , Seoul , Korea.|c Brain Korea 21 Project for Medical Science , Yonsei University , Seoul , Korea.
Yong Dae
YD
Shin
b Division of Rheumatology, Department of Internal Medicine , Institute for Immunology and Immunological Disease, Yonsei University College of Medicine , Seoul , Korea.|c Brain Korea 21 Project for Medical Science , Yonsei University , Seoul , Korea.|d Severance Biomedical Institute, Yonsei University College of Medicine , Seoul, Korea.
Yong-Beom
YB
Park
a Department of Prosthodontics, Oral Science Research Center, BK21 Plus Project , Yonsei University College of Dentistry , Seoul , Korea.
Young-Bum
YB
Park
D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D015921:Dental Implants; D005347:Fibroblasts / Q000502:physiology*; D005881:Gingiva / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism*; D010012:Osteogenesis; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology
D015921:Dental Implants; D014025:Titanium
Induced pluripotent stem cells derived from dental stem cells; characterization; osteogenesis; rough surface; titanium disc