miR-448 is a novel prognostic factor of lung squamous cell carcinoma and regulates cells growth and metastasis by targeting DCLK1.
MicroRNA-448 (miR-448) has been showed to be low-expressed and function as tumor suppressor in most human cancers. However, there are limited reports on the clinical significance and biological function of miR-448 in lung squamous cell carcinoma. In this study, we observed that miR-448 expression was decreased in lung squamous cell carcinoma tissues and cell lines. Meanwhile, miR-448 expression associated with differentiated degree, T classification (tumor size), N classification (lymph node metastasis), M classification (distant metastasis), clinical stage and prognosis of lung squamous cell carcinoma patients. In survival analysis, low expression of miR-448 was a poor independent prognostic factor for lung squamous cell carcinoma patients. Moreover, gain-of-function and loss-of-function studies showed miR-448 acted as a tumor suppressor regulating lung squamous cell carcinoma cells growth and metastasis. Furthermore, DCLK1 has been identified as a potential target for miR-448 to regulate lung squamous cell carcinoma cells growth and metastasis. In conclusion, miR-448 low-expression was a poor prognostic factor for lung squamous cell carcinoma patients, and miR-448 served as a tumor suppressor in lung squamous cell carcinoma cells via targeting DCLK1.
Department of Respiratory Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong, 272019, China.
Changting
C
Shan
Department of Respiratory Medicine, Huai'an Second People's Hospital, Huai'an, Jiangsu, 223002, China.
Fan
F
Fei
Department of Paediatric Surgery, Jining No.1 People Hospital, Jining, Shandong, 272011, China.
Fengzhu
F
Li
Department of Paediatrics, Jining No.1 People Hospital, Jining, Shandong, 272011, China.
Bo
B
Zhuang
Department of Respiratory Medicine, Huai'an Second People's Hospital, Huai'an, Jiangsu, 223002, China.
Yulong
Y
Zheng
Department of Respiratory Medicine, Huai'an Second People's Hospital, Huai'an, Jiangsu, 223002, China.
Yufeng
Y
Wan
Department of Respiratory Medicine, Huai'an Second People's Hospital, Huai'an, Jiangsu, 223002, China. Electronic address: chenjianhui@hainan.com.
Jianhui
J
Chen
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D000089742:Doublecortin-Like Kinases; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
D047908:Intracellular Signaling Peptides and Proteins; C557400:MIRN448 microRNA, human; D035683:MicroRNAs; C117347:DCLK1 protein, human; D000089742:Doublecortin-Like Kinases; D017346:Protein Serine-Threonine Kinases
Lung cancer; Lung squamous cell carcinoma; Metastasis; Proliferation; miR-448; microRNA