Synthesis, Characterization, and Biological Evaluation of Nanostructured Hydroxyapatite with Different Dimensions.
Nanosized hydroxyapatite (HA) is a promising candidate for a substitute for apatite in bone in biomedical applications. Furthermore, due to its excellent bone bioactivity, nanosized strontium-substituted HA (SrHA) has aroused intensive interest. However, the size effects of these nanoparticles on cellular bioactivity should be considered. In this study, nanosized HA and SrHA with different dimensions and crystallization were synthesized by hydrothermal methods. The phase, crystallization and chemical composition were analyzed by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR), respectively. The morphology was observed under field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The degradation behaviors of the samples were monitored by determining the ions release profile with inductively coupled plasma mass spectrometry (ICP-MS). The releasing behavior of Ca2+and Sr2+showed that the degradation rate was proportional to the specific surface area and inversely proportional to crystallization. The in vitro experiment evaluated by MG63 cells showed that SrHA nanorods with a length greater than 100 nm had the best biological performance both in cell proliferation and differentiation (*p< 0.05 compared with HA-1 and SrHA-1; *p< 0.01 compared with HA-2). In addition, HA nanoparticles with a lower aspect ratio had better bioactivity than higher ones (*p< 0.05). This study demonstrated that nanosized HA and SrHA with subtle differences (including dimensions, crystallization, specific surface area, and degradation rate) could affect the cellular growth and thus might have an impact on bone growth in vivo. This work provides a view of the role of nano-HAs as ideal biocompatible materials in future clinical applications.
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. nanboshan1987@163.com.
Zhen
Z
Geng
School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China. m13752208393@163.com.
Qin
Q
Yuan
Department of Spinal Surgery, Liuzhou Worker's Hospital, Liuzhou 545001, China. doctorzhuo@139.com.
Xianglong
X
Zhuo
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. zyli@tju.edu.cn.|School of Materials Science and Engineering, Tianjin University, 92 Weijin Road, Tianjin 300072, China. zyli@tju.edu.cn.
Zhaoyang
Z
Li
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. zdcui@tju.edu.cn.
Zhenduo
Z
Cui
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. slzhu@tju.edu.cn.
Shengli
S
Zhu
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. yqliang@tju.edu.cn.
Yanqin
Y
Liang
School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China. yundeliu@126.com.
Yunde
Y
Liu
School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China. kris_10713@126.com.
Huijing
H
Bao
School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China. nicolelea@163.com.
Xue
X
Li
School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China. huo.qianyu@163.com.
Qianyu
Q
Huo
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. xjyang@tju.edu.cn.|School of Materials Science and Engineering, Tianjin University, 92 Weijin Road, Tianjin 300072, China. xjyang@tju.edu.cn.
Xianjin
X
Yang
absorbable implants; biocompatible materials; crystallization; nanostructured materials; surface properties