Acute toxicity profiling of the ethyl acetate fraction ofSwietenia macrophyllaseeds andin-vitroneuroprotection studies.
Swietenia macrophylla(SM) is a medicinally important plant found in tropical and subtropical regions of the world. The ethyl acetate fraction of the seeds ofS. macrophylla(SMEAF) is reported to exhibit potent anticancer, antitumor, anti-inflammatory and antifeedant activities. Till date, there have been no studies reported on the acute oral toxicity profile of the ethyl acetate fraction of the seeds of SM. The objective of the present study was to determine the acute toxicity of SMEAF and evaluate thein-vitroneuroprotective activity of SMEAF using primary neuronal cell cultures. In acute oral toxicity study, the SMEAF did not produce any lethal signs of morbidity and mortality. Histo-pathological findings, support the safety of SMEAF, as there were no significant changes observed in any of the parameters studied. Based on the results obtained in MTT assay, we infer that SMEAF has a significant neuroprotective effect, as it increased the cell viability and exhibited protection to the neuronal cells against TBHP induced oxidative stress. Thus, SMEAF can be suggested for use in the development of herbal drug formulations with neuroprotective potential.
Department of Medical Genetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia; MAKNA Cancer Research Laboratory, Institute of Biosciences, Universiti Putra Malaysia, Malaysia.
Mustak
M
Sayyad
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, Malaysia.
Ning
N
Tiang
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, Malaysia.
Yatinesh
Y
Kumari
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, Malaysia.
Bey Hing
BH
Goh
Center for Excellence in Post Harvest Technologies, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 2808, USA.
Yogini
Y
Jaiswal
Department of Medical Genetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia; MAKNA Cancer Research Laboratory, Institute of Biosciences, Universiti Putra Malaysia, Malaysia.
Rozita
R
Rosli
Center for Excellence in Post Harvest Technologies, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 2808, USA.
Leonard
L
Williams
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, Malaysia.
Mohd Farooq
MF
Shaikh
Acute oral toxicity; Ethyl acetate extract; Neuroprotection; Oxidative stress; Swietenia macrophylla