Protective manifestation of bacoside A and bromelain in terms of cholinesterases, gamma-amino butyric acid, serotonin level and stress proteins in the brain of dichlorvos-intoxicated mice.
The objective of the study was to evaluate the neuroprotective effects of bacoside A and bromelain against dichlorvos-incited toxicity. Healthy 6-8-week old, male Swiss mice were administered subacute doses of dichlorvos (40 mg/kg bw), bacoside A (5 mg/kg bw) and bromelain (70 mg/kg bw). AChE, BChE, GABA, serotonin and total protein content and their expressions were used for determination of toxic action of dichlorvos. Protective effects of bacoside A and bromelain were evaluated on the same parameters. Exposure to dichlorvos leads to significant decline in activities of AChE (p < 0.01, p < 0.001), BChE (p < 0.05) and GABA (p < 0.01) and total protein levels (p < 0.01). Antioxidant treatment significantly increased the activities of AChE (p < 0.01, p < 0.001), BChE (p < 0.05), GABA (p < 0.01) and total protein level (p < 0.05) compared to those in dichlorvos-treated mice. Overexpression of Hsp 70 protein and underexpression of phosphorylase a and b, catalase SOD and GPx were observed after dichlorvos exposure which suggests the oxidative stress. The results indicate that dichlorvos-induced neuronal damage which results in the generation of molecular expression of proteins is in agreement with the biochemical data ameliorated by bacoside A and bromelain.
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, 304022, India.
Bharti
B
Chaudhary
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, 304022, India. renu_bisht22@yahoo.co.in.
Renu
R
Bist
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001963:Bromelains / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism*; D004006:Dichlorvos / Q000633:toxicity*; D015536:Down-Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010762:Phosphorylase a / Q000378:metabolism; D010763:Phosphorylase b / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D020011:Protective Agents; D012503:Saponins; D014315:Triterpenes; C500175:bacoside A; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D004006:Dichlorvos; D001963:Bromelains; D002374:Catalase; D013482:Superoxide Dismutase; D010762:Phosphorylase a; D010763:Phosphorylase b; D002802:Cholinesterases
Bacoside A; Bromelain; Cholinergic circuit; Dichlorvos; Neurotransmitter