Towards optimization of odonto/osteogenic bioengineering: in vitro comparison of simvastatin, sodium fluoride, melanocyte-stimulating hormone.
Tissue engineering has emerged as a potential therapeutic option for dental problems in recent years. One of the policies in tissue engineering is to use both scaffolds and additive factors for enhancing cell responses. This study aims to evaluate and compare the effect of three types of biofactors on poly-caprolactone-poly-ethylene glycol-poly caprolactone (PCL-PEG-PCL) nanofibrous scaffold on human dental pulp stem cell (hDPSCs) engineering. The PCL-PEG-PCL copolymer was synthesized with ring opening polymerization method, and its nanofiber scaffold was prepared by electrospinning method. Nanofibrous scaffold-seeded hDPSCs were treated with sodium fluoride (NaF), melanocyte-stimulating hormone (MSH), or simvastatin (SIM). Non-treated nanofiber seeded cells were utilized as control. The viability, biocompatibility, adhesion, proliferation rate, morphology, osteo/odontogenic potential, and the expression of tissue-specific genes were studied. The results showed that significant higher results demonstrated significant higher adhesive behavior, viability, alizarin red activity, and dentin specific gene expression in MSH- and SIM-treated cells (p < 0.05). This study is unique; in that, it compares the effects of different treatments for optimization of dental tissue engineering.
Dental Department, Behbood Hospital, Tabriz, Iran.
Vahid
V
Zijah
Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Roya
R
Salehi
Oral Medicine Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Marziyeh
M
Aghazadeh
Endodontics Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
Mohammad
M
Samiei
Stem Cell Research Center and Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. alizadehe@tbzmed.ac.ir.|Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. alizadehe@tbzmed.ac.ir.
Effat
E
Alizadeh
Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. davaran@tbzmed.ac.ir.|Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. davaran@tbzmed.ac.ir.
Soodabeh
S
Davaran
D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D003782:Dental Pulp / Q000187:drug effects / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D019821:Simvastatin / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
D011091:Polyesters; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D011092:Polyethylene Glycols; D012969:Sodium Fluoride; D009074:Melanocyte-Stimulating Hormones; D019821:Simvastatin
Human dental pulp stem cells; Melanocyte-stimulating hormone; Simvastatin; Sodium fluoride