Inhibitory Activities ofStauntonia hexaphyllaLeaf Constituents on Rat Lens Aldose Reductase and Formation of Advanced Glycation End Products and Antioxidant.
Stauntonia hexaphylla(Thunb.) Decne. (Lardizabalaceae) leaves (SHL) have been used traditionally as analgesics, sedatives, diuretics, and so on, in China. To date, no data have been reported on the inhibitory effect of SHL and its constituents on rat lens aldose reductase (RLAR) and advanced glycation end products (AGEs). Therefore, the inhibitory effect of compounds isolated from SHL extract on RLAR and AGEs was investigated to evaluate potential treatments of diabetic complications. The ethyl acetate (EtOAC) fraction of SHL extract showed strong inhibitory activity on RLAR and AGEs; therefore, EtOAc fraction (3.0 g) was subjected to Sephadex LH-20 column chromatography, for further fractionation, with 100% MeOH solvent system to investigate its effect on RLAR and AGEs. Phytochemical investigation of SHL led to the isolation of seven compounds. Among the isolated compounds, chlorogenic acid, calceolarioside B, luteolin-3'-O-β-D-glucopyranoside, quercetin-3-O-β-D-glucopyranoside, and luteolin-7-O-β-D-glucopyranoside exhibited significant inhibitory activity against RLAR with IC50in the range of 7.34-23.99μM. In addition, 3-(3,4-dihydroxyphenyl) propionic acid, neochlorogenic acid, and luteolin-3'-O-β-D-glucopyranoside exhibited the most potent inhibitory activity against formation of AGEs, with an IC50value of 115.07-184.06μM, compared to the positive control aminoguanidine (820.44μM). Based on these findings, SHL dietary supplements could be considered for the prevention and/or treatment of diabetes complication.
Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea.
Seung Hwan
SH
Hwang
Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea.
Shin Hwa
SH
Kwon
Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea.
Set Byeol
SB
Kim
Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea; Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea; Institute of Korean Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea.
Soon Sung
0000-0002-9464-4127
SS
Lim
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D002681:China; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006801:Humans; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D064028:Tracheophyta / Q000737:chemistry*
D000975:Antioxidants; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D000449:Aldehyde Reductase