Porcine Epidemic Diarrhea Virus Induces Autophagy to Benefit Its Replication.
The new porcine epidemic diarrhea (PED) has caused devastating economic losses to the swine industry worldwide. Despite extensive research on the relationship between autophagy and virus infection, the concrete role of autophagy in porcine epidemic diarrhea virus (PEDV) infection has not been reported. In this study, autophagy was demonstrated to be triggered by the effective replication of PEDV through transmission electron microscopy, confocal microscopy, and Western blot analysis. Moreover, autophagy was confirmed to benefit PEDV replication by using autophagy regulators and RNA interference. Furthermore, autophagy might be associated with the expression of inflammatory cytokines and have a positive feedback loop with the NF-κB signaling pathway during PEDV infection. This work is the first attempt to explore the complex interplay between autophagy and PEDV infection. Our findings might accelerate our understanding of the pathogenesis of PEDV infection and provide new insights into the development of effective therapeutic strategies.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. guoxiaozhen@webmail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. guoxiaozhen@webmail.hzau.edu.cn.
Xiaozhen
X
Guo
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. zhangmengjia@webmail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. zhangmengjia@webmail.hzau.edu.cn.
Mengjia
M
Zhang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. 632071039@webmail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. 632071039@webmail.hzau.edu.cn.
Xiaoqian
X
Zhang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. wstx1992@163.com.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. wstx1992@163.com.
Xin
X
Tan
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. 15071194245@163.com.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. 15071194245@163.com.
Hengke
H
Guo
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. aiyouwei0620@163.com.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. aiyouwei0620@163.com.
Wei
W
Zeng
The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. gkyangk@163.com.|College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China. gkyangk@163.com.
Guokai
G
Yan
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Memonatta80@webmail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. Memonatta80@webmail.hzau.edu.cn.
Atta Muhammad
AM
Memon
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. lzh1990@webmail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. lzh1990@webmail.hzau.edu.cn.
Zhonghua
Z
Li
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. yingzizhu10@163.com.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. yingzizhu10@163.com.
Yinxing
Y
Zhu
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. abing0313@webmail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. abing0313@webmail.hzau.edu.cn.
Bingzhou
B
Zhang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. kuxugang84@163.com.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. kuxugang84@163.com.
Xugang
X
Ku
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. wumeizhou@mail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. wumeizhou@mail.hzau.edu.cn.
Meizhou
M
Wu
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. fanshengxian@mail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. fanshengxian@mail.hzau.edu.cn.
Shengxian
S
Fan
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. he628@mail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. he628@mail.hzau.edu.cn.
Qigai
Q
He
D000818:Animals; D001343:Autophagy; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D054884:Host-Pathogen Interactions; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053485:Porcine epidemic diarrhea virus / Q000502:physiology*; D014709:Vero Cells; D014779:Virus Replication
PEDV replication; apoptosis; autophagy machinery; inflammatory responses