Investigation on metal tolerance and phytoremoval activity in the poplar hybrid clone "Monviso" under Cu-spiked water: Potential use for wastewater treatment.
A serious concern for the environmental and human health is represented by the increasing copper (Cu) occurrence in agricultural soils and waters, because of the possible food contamination and bioaugmentation along the trophic chain. The request for the decontamination of different matrices with an environmentally sustainable technology as the phytoremediation should be addressed by selecting plant materials with improved pollutant tolerance and removal capability. With this purpose, plants of the hybrid poplar clone "Monviso" (Populus×generosa A. Henry×P. nigra L.) were grown in growth chamber under hydroponics and exposed to excess Cu concentrations (T1, 75μM Cu; T2, 150μM Cu), selected as about 5 and 10 times higher than those allowed by the Italian regulation on water use. Results evidenced a notable Cu tolerance by poplar plants, particularly at the lowest Cu concentration. At organ level, the root system was the most affected by Cu treatment, especially in T2-exposed plants. Copper determinations revealed that the metal was mostly bioaccumulated in the roots, with a limited amount reaching the shoots. Chlorophyll content and fluorescence analyses confirmed the visible symptoms in leaves, highlighting a good physiological status in T1-exposed plants. Contrarily, an impairment of the main processes associated to photosynthesis was observed in T2-exposed plants also by gas exchange measurements. Remarkably, the Cu content analysis of the spiked water solutions revealed that poplar plants succeeded in removing almost the 50% of the total Cu amount added. These results strengthen the evidence that poplar plants represent a useful eco-friendly bio-tool for the decontamination of metal polluted waters.
Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo (RM), Italy.
Fabrizio
F
Pietrini
Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo (RM), Italy.
Daniela
D
Di Baccio
Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo (RM), Italy.
Valentina
V
Iori
Institute of Genetics, Physiology and Plant Protection, Academy of Science of Moldova, Chisinau, Moldova.
Sofia
S
Veliksar
Institute of Genetics, Physiology and Plant Protection, Academy of Science of Moldova, Chisinau, Moldova.
Natalia
N
Lemanova
Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, Kaunas, Lithuania.
Liudvika
L
Juškaitė
Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, Kaunas, Lithuania.
Audrius
A
Maruška
Institute of Agroenvironmental and Forest Biology, National Research Council, Monterotondo (RM), Italy. Electronic address: massimo.zacchini@ibaf.cnr.it.
Massimo
M
Zacchini
D001673:Biodegradation, Environmental; D003300:Copper / Q000302:isolation & purification*; D008670:Metals; D018517:Plant Roots / Q000378:metabolism; D032107:Populus / Q000378:metabolism*; D062065:Wastewater; D018508:Water Purification
D003300:Copper; D008670:Metals; D062065:Wastewater
Chlorophyll fluorescence; Heavy metal; Photosynthesis; Phytoremediation; Water pollution