Melanin-Associated Synthesis of SERS-Active Nanostructures and the Application for Monitoring of Intracellular Melanogenesis.
Melanin plays an indispensable role in the human body. It serves as a biological reducer for the green synthesis of precious metal nanoparticles. Melanin-Ag nanocomposites were successfully produced which exhibited very strong surface-enhanced Raman scattering (SERS) effect because of the reducibility property of melanin. A melanin-Ag composite structure was synthesized in situ in melanin cells, and SERS technique was performed for the rapid imaging and quantitative assay of intracellular melanin. This imaging technique was also used to successfully trace the formation and secretion of intracellular melanin after stimulation with melanin-stimulating hormones. Based on the self-reducing property of melanin, the proposed SERS imaging method can provide potentially powerful analytical detection tools to study the biological functions of melanin and to prevent and cure melanin-related diseases.
SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou 510631, China. dhx98@sina.com.
Haixin
H
Dong
SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Liuzm021@126.com.
Zhiming
Z
Liu
SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou 510631, China. honghq@scnu.edu.cn.
Huiqing
H
Zhong
Infinitus (China) Company Ltd., Guangzhou 510665, China. Youky.Yang@infinitus-int.com.
Hui
H
Yang
Infinitus (China) Company Ltd., Guangzhou 510665, China. dhx98@tom.com.
Yan
Y
Zhou
SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou 510631, China. 15322072000@163.com.
Yuqing
Y
Hou
Infinitus (China) Company Ltd., Guangzhou 510665, China. Jia.Long@infinitus-int.com.
Jia
J
Long
Infinitus (China) Company Ltd., Guangzhou 510665, China. Jin.Lin@infinitus-int.com.
Jin
J
Lin
SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou 510631, China. ann@scnu.edu.cn.
Zhouyi
Z
Guo
bioimaging; melanin–Ag nanocomposites; melanogenesis; surface-enhanced Raman scattering