A new amplified π-shape electrochemical aptasensor for ultrasensitive detection of aflatoxin B1.
There is a prompt need for determination of aflatoxin B1(AFB1) in food products to avoid distribution and consumption of contaminated food products. In this study, an accurate electrochemical sensing strategy was presented for detection of AFB1based on aptamer (Apt)-complementary strands of aptamer (CSs) complex which forms a π-shape structure on the surface of electrode and exonuclease I (Exo I). The presence of π-shape structure as a double-layer physical barrier allowed detection of AFB1with high sensitivity. In the absence of AFB1, the π-shape structure remained intact, so only a weak peak current was recorded. Upon the addition of AFB1, the π-shape structure was disassembled and a strong current was recorded following the addition of Exo I. Under optimal conditions, the electrochemical signals enhanced as AFB1concentrations increased with a dynamic range of 7-500pg/mL and a limit of detection (LOD) of 2pg/mL. The developed aptasensor was also used to analyze AFB1spiked human serum and grape juice samples and the recoveries were 95.4-108.1%.
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Khalil
K
Abnous
Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Research Institute of Sciences and New Technology, Mashhad, Iran.
Noor Mohammad
NM
Danesh
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Mona
M
Alibolandi
Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad
M
Ramezani
Cardiovascular Research Center, Ghaem hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Ahmad
A
Sarreshtehdar Emrani
Department of Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Reza
R
Zolfaghari
Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: taghdisihm@mums.ac.ir.
Seyed Mohammad
SM
Taghdisi
D016604:Aflatoxin B1 / Q000097:blood* / Q000235:genetics; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry
D052157:Aptamers, Nucleotide; D006046:Gold; D016604:Aflatoxin B1
Aflatoxin B(1); Electrochemical aptasensor; Exonuclease I; Real samples; π-shape