Bromelain capped gold nanoparticles as the novel drug delivery carriers to aggrandize effect of the antibiotic levofloxacin.
To develop bromelain capped gold nanoparticles (BRN capped Au-NPs) as the effective drug delivery carriers of the antibiotic levofloxacin (LvN) and evaluate antibacterial potential of its bioconjugated form compared to pure LvN. BRN capped Au-NPs were synthesized byin vitromethod and bioconjugated to LvN using 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide as activator to form Au-BRN-LvN-NPs. These were characterized for mean particle size by dynamic light scattering analysis, zeta potential by Zetasizer nanosystem analysis and transmission electron microscopy (TEM) on carbon coated TEM copper grids by TEM respectively. Drug loading efficiency of LvN was calculated using UV-visible spectroscopy by standard curve of pure LvN. Antibacterial efficacy of Au-BRN-LvN-NPs and pure LvN was determined by evaluating minimum inhibitory concentration (MIC) againstStaphylococcus aureusandEschereschia coli. Two peaks were observed in Au-BRN-LvN-NPs spectrum one at 307 nm and other at 526 nm while one peak in BRN capped Au-NPs at 522 nm during UV spectroscopy suggesting red shift. The drug loading efficiency of LvN was found to be 84.8 ± 2.41 %. The diameter of Au-BRN-LvN-NPs and BRN capped Au-NPs were found to be (58.65 ± 2 nm, 38.11 ± 2 nm), zeta potential (-9.01 mV, -13.8 mV) and surface morphology (~13.2 nm, 11.4 nm) respectively. The MICs againstS. aureusandE. coliwere found to be (0.128 µg/mL, 1.10 µg/mL) for Au-BRN-LvN-NPs and (0.547 µg/mL, 1.96 µg/mL) for pure LvN. The results suggested that BRN capped Au-NPs can be used as effective drug delivery carriers of the antibiotic LvN. The Au-BRN-LvN-NPs exhibited enhanced antibacterial activity compared to pure LvN alone. (Graphical abstract see Figure 1(Fig. 1)).
Department of Pharmacy, Integral University, Lucknow, Uttar Pradesh 226026 (India).
Paramdeep
P
Bagga
Department of Pharmacy, Integral University, Lucknow, Uttar Pradesh 226026 (India).
Tarique Mahmood
TM
Ansari
Department of Pharmacy, Integral University, Lucknow, Uttar Pradesh 226026 (India).
Hefazat Hussain
HH
Siddiqui
Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.
Asad
A
Syed
Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.
Ali H
AH
Bahkali
Department of Pharmacy, Integral University, Lucknow, Uttar Pradesh 226026 (India).
Md Azizur
MA
Rahman
Nanotechnology Lab, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026 (India).
Mohd Sajid
MS
Khan
antibacterial activity; bromelain; gold nanoparticles; levofloxacin; novel drug delivery carriers