Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits.
We aimed to determine the role of estrogens in modulating the size of neuronal somata of paravaginal ganglia. Rabbits were allocated into control (C), ovariectomized (OVX), and OVX treated with estradiol benzoate (OVX + EB) groups to evaluate the neuronal soma area; total serum estradiol (E2) and testosterone (T) levels; the percentage of immunoreactive (ir) neurons anti-aromatase, anti-estrogen receptor (ERα, ERβ) and anti-androgen receptor (AR); the intensity of the immunostaining anti-glial cell line-derived neurotrophic factor (GDNF) and the GDNF family receptor alpha type 1 (GFRα1); and the number of satellite glial cells (SGCs) per neuron. There was a decrease in the neuronal soma size for the OVX group, which was associated with low T, high percentages of aromatase-ir and neuritic AR-ir neurons, and a strong immunostaining anti-GDNF and anti-GFRα1. The decrease in the neuronal soma size was prevented by the EB treatment that increased the E2 without affecting the T levels. Moreover, there was a high percentage of neuritic AR-ir neurons, a strong GDNF immunostaining in the SGC, and an increase in the SGCs per neuron. Present findings show that estrogens modulate the soma size of neurons of the paravaginal ganglia, likely involving the participation of the SGC.
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala (UATx), Tlaxcala, TLAX, Mexico.
Laura G
LG
Hernández-Aragón
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala (UATx), Tlaxcala, TLAX, Mexico.
Verónica
V
García-Villamar
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala (UATx), Tlaxcala, TLAX, Mexico.
María de Los Ángeles
ML
Carrasco-Ruiz
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala (UATx), Tlaxcala, TLAX, Mexico.
Leticia
L
Nicolás-Toledo
Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, CDMX, Mexico.
Arturo
A
Ortega
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala (UATx), Tlaxcala, TLAX, Mexico.
Estela
E
Cuevas-Romero
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala (UATx), Tlaxcala, TLAX, Mexico; Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), Unidad Foránea Tlaxcala, Tlaxcala, TLAX, Mexico.
Margarita
M
Martínez-Gómez
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala (UATx), Tlaxcala, TLAX, Mexico; Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), Unidad Foránea Tlaxcala, Tlaxcala, TLAX, Mexico.
Francisco
0000-0002-9835-3335
F
Castelán
D000818:Animals; D001141:Aromatase / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D004967:Estrogens / Q000502:physiology*; D005260:Female; D005724:Ganglia / Q000166:cytology*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D010052:Ovariectomy; D011817:Rabbits; D011944:Receptors, Androgen / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D014621:Vagina / Q000294:innervation*
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D051100:Glial Cell Line-Derived Neurotrophic Factor; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D011944:Receptors, Androgen; C074283:estradiol 3-benzoate; D013739:Testosterone; D004958:Estradiol; D001141:Aromatase