Protein degradation systems in the skeletal muscles of parr and smolt Atlantic salmon Salmo salar L. and brown trout Salmo trutta L.
Although protein degradation limits the rate of muscle growth in fish, the role of proteolytic systems responsible for degrading myofibrillar proteins in skeletal muscle is not well defined. The study herein aims to evaluate the role of calpains (calcium-activated proteases) and proteasomes (ATP-dependent proteases) in mediating muscle protein turnover at different life stages in wild salmonids. Protease activities were estimated in Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.) parr and smolts from the Indera River (Kola Peninsula, Russia). Calpain and proteasome activities in Atlantic salmon skeletal muscles were lower in smolts as compared with parr. Reduced muscle protein degradation accompanying Atlantic salmon parr-smolt transformation appeared to provide intense muscle growth essential for a minimum threshold size achievement that is required for smoltification. Calpain and proteasome activities in brown trout parr and smolts at age 3+ did not significantly differ. However, calpain activity was higher in smolts brown trout 4+ as compared with parr, while proteasome activity was lower. Results suggest that brown trout smoltification does not correspond with intense muscle growth and is more facultative and plastic in comparison with Atlantic salmon smoltification. Obtained data on muscle protein degradation capacity as well as length-weight parameters of fish reflect differences between salmon and trout in growth and smoltification strategies.
Institute of Biology, Karelian Research Centre of Russian Academy of Sciences, Pushkinskaya Str., 11, Petrozavodsk, Russian Federation, 185910. nkantserova@yandex.ru.
Nadezda P
NP
Kantserova
Institute of Biology, Karelian Research Centre of Russian Academy of Sciences, Pushkinskaya Str., 11, Petrozavodsk, Russian Federation, 185910.
Liudmila A
LA
Lysenko
Institute of Biology, Karelian Research Centre of Russian Academy of Sciences, Pushkinskaya Str., 11, Petrozavodsk, Russian Federation, 185910.
Alexey E
AE
Veselov
Institute of Biology, Karelian Research Centre of Russian Academy of Sciences, Pushkinskaya Str., 11, Petrozavodsk, Russian Federation, 185910.
Nina N
NN
Nemova
D000375:Aging; D000818:Animals; D005260:Female; D029941:Fish Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D010447:Peptide Hydrolases / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D059748:Proteolysis; D012487:Salmonidae / Q000254:growth & development* / Q000502:physiology*
D029941:Fish Proteins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex
Atlantic salmon; Brown trout; Calpain; Proteasome; Protein degradation; Smoltification