Shape-dependent enzyme-like activity of Co3O4nanoparticles and their conjugation with his-tagged EGFR single-domain antibody.
In this study, Co3O4nanopolyhedrons, nanocubes, nanoplates and nanorods were synthesized and characterized. Furthermore, the peroxidase- and catalase-like activities of these Co3O4nanoparticles (NPs) were studied and influence of the exposed crystal planes was explored. According to their morphology and peroxidase-like activity, dimercaptosuccinic acid (DMSA) modified Co3O4nanopolyhedrons synthesized via coprecipitation method (Co3O4NHs) were selected as a proper candidate for the immunohistochemical (IHC) detection of epidermal growth factor receptor (EGFR) expression in non-small cell lung cancer (NSCLC) tissues. Bivalent cobalt ions were coupled to the carboxyls on the surface of the obtained Co3O4NHs so as to chelate the hexahistidine residues (His-Tags) at the C-terminal of EGFR single-domain antibodies (EGFR sdAbs). Finally, the as-obtained EGFR sdAbs-binding Co3O4NHs (Co3O4nanoprobes) were successfully applied to the detection of EGFR expression in NSCLC tissues.
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing 210096, PR China.
Wei
W
Zhang
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
Jinlai
J
Dong
Research Centre of Clinical Oncology, Jiangsu Cancer Hospital, Nanjing 210009, PR China.
Yang
Y
Wu
Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Laboratory of Cellular and Molecular Biology, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, PR China.
Peng
P
Cao
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing 210096, PR China.
Lina
L
Song
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing 210096, PR China.
Ming
M
Ma
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing 210096, PR China. Electronic address: guning@seu.edu.cn.
Ning
N
Gu
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Nanjing 210096, PR China. Electronic address: zhangyu@seu.edu.cn.
Yu
Y
Zhang
D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism; D003035:Cobalt / Q000737:chemistry*; D066246:ErbB Receptors / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008175:Lung Neoplasms / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D061905:Single-Domain Antibodies / Q000737:chemistry* / Q000276:immunology
D058726:Nanoconjugates; D010087:Oxides; D061905:Single-Domain Antibodies; D003035:Cobalt; C512478:EGFR protein, human; D066246:ErbB Receptors; C060728:cobalt oxide
Co(3)O(4) nanoparticles; Crystal plane; His-Tag; Immunohistochemistry; Peroxidase