Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations.
Ion mobility (IM) separations have a broad range of analytical applications, but insufficient resolution often limits their utility. Here, we report on ion mobility separations in a structures for lossless ion manipulations (SLIM) serpentine ultralong path with extended routing (SUPER) traveling wave (TW) ion mobility (IM) module in conjunction with mass spectrometry (MS). Ions were confined in the SLIM by rf fields in conjunction with a DC guard bias, enabling essentially lossless TW transmission over greatly extended paths. The extended routing utilized multiple passes (e.g., ∼1094 m over 81 passes through the 13.5 m serpentine path) and was facilitated by the introduction of a lossless ion switch that allowed ions to be directed to either the MS detector or for another pass through the serpentine separation region, allowing theoretically unlimited IM path lengths. The multipass SUPER IM-MS provided resolution approximately proportional to the square root of the number of passes (or total path length). More than 30-fold higher IM resolution (∼340 vs ∼10) for Agilent tuning mix m/z 622 and 922 ions was achieved for 40 passes compared to commercially available drift tube IM and other TWIM-based platforms. An initial evaluation of the isomeric sugars lacto-N-hexaose and lacto-N-neohexaose showed the isomeric structures to be baseline resolved, and a new conformational feature for lacto-N-neohexaose was revealed after 9 passes. The new SLIM SUPER high resolution TWIM platform has broad utility in conjunction with MS and is expected to enable a broad range of previously challenging or intractable separations.
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Liulin
L
Deng
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Ian K
IK
Webb
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Sandilya V B
SVB
Garimella
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Ahmed M
AM
Hamid
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Xueyun
0000-0001-9782-4521
X
Zheng
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Randolph V
RV
Norheim
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Spencer A
SA
Prost
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Gordon A
GA
Anderson
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Jeremy A
JA
Sandoval
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Erin S
0000-0001-5246-2213
ES
Baker
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Yehia M
0000-0001-6085-193X
YM
Ibrahim
Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Boulevard, P.O. Box 999, MSIN K8-98, Richland, Washington 99352, United States.
Richard D
0000-0002-2381-2349
RD
Smith
D006801:Humans; D000075663:Ion Mobility Spectrometry / Q000379:methods*; D007477:Ions / Q000737:chemistry; D007536:Isomerism; D008895:Milk, Human / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry
D007477:Ions; D009844:Oligosaccharides; C046434:lacto-N-hexaose