Comparison of Data from a Single-Analyte and a Multianalyte Method for Determination of Urinary Total Deoxynivalenol in Human Samples.
Deoxynivalenol (DON) exposure is estimated by the combined measures of urinary DON and DON-glucuronides. In this study, data from single-mycotoxin (SM) and a multimycotoxin (MM) methods were compared for 256 Swedish adult urine samples. Both methods included β-glucuronidase predigestion, immunoaffinity enrichment, and LC-MS/MS. However, the specific reagents, apparatus, and conditions were not identical in part because the MM method measures additional mycotoxins. DON was detected in 88 and 63% of samples using the SM and MM methods, respectively, with the following mean and median concentrations: SM, mean = 5.0 ng/mL, SD = 7.4, range of positives = 0.5-60.2 ng/mL, median = 2.5 ng/mL, IQR = 1.0-5.5 ng/mL; MM, mean = 4.4 ng/mL, SD = 12.9, range of positives = 0.5-135.2 ng/mL, median = 0.8 ng/mL, IQR = 0.3-3.5. Linear regression showed a significant, albeit modest, correlation between the two measures (p = 0.0001, r = 0.591). The differences observed may reflect subtle handling differences in DON extraction and quantitation between the methods.
Maryland Institute for Applied Environmental Health, School of Public Health, University of Maryland , College Park, Maryland, United States.
Paul C
http://orcid.org/0000-0001-7744-891X
PC
Turner
Institute of Sciences of Food Production (ISPA) of the National Research Council (CNR) , Bari, Italy.
Michele
http://orcid.org/0000-0002-5698-4921
M
Solfrizzo
Maryland Institute for Applied Environmental Health, School of Public Health, University of Maryland , College Park, Maryland, United States.
Allison
A
Gost
Institute of Sciences of Food Production (ISPA) of the National Research Council (CNR) , Bari, Italy.
Lucia
L
Gambacorta
The National Food Agency , Box 622, SE 751 26 Uppsala, Sweden.
Monica
M
Olsen
The National Food Agency , Box 622, SE 751 26 Uppsala, Sweden.
Stina
S
Wallin
The National Food Agency , Box 622, SE 751 26 Uppsala, Sweden.
Natalia
N
Kotova
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009183:Mycotoxins / Q000652:urine; D010865:Pilot Projects; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014255:Trichothecenes / Q000652:urine*; D055815:Young Adult
D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol
deoxynivalenol; exposure; multimycotoxin method; single-mycotoxin method