Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22.
Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.
Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Ni Putu Desy
NPD
Aryantini
Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Daisuke
D
Kondoh
Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
Keita
K
Nishiyama
Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
Yuji
Y
Yamamoto
Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
Takao
T
Mukai
Integrated Laboratory for Bioscience and Biotechnology, Udayana University, Bukit Jimbaran Campus, Badung, Bali 80361, Indonesia.
I Nengah
IN
Sujaya
Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Tadasu
T
Urashima
Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Kenji
K
Fukuda
D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007150:Immunohistochemistry; D052201:Lacticaseibacillus rhamnosus / Q000187:drug effects / Q000502:physiology*; D007797:Laminin / Q000378:metabolism*; D018021:Lithium Chloride / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D007797:Laminin; D008565:Membrane Proteins; D018021:Lithium Chloride
cell surface proteins; host–microbial interactions; lactic acid bacteria; laminin; probiotics; ribosomal proteins