Characterization of the Soluble NSF Attachment Protein gene family identifies two members involved in additive resistance to a plant pathogen.
Proteins with Tetratricopeptide-repeat (TPR) domains are encoded by large gene families and distributed in all plant lineages. In this study, the Soluble NSF-Attachment Protein (SNAP) subfamily of TPR containing proteins is characterized. In soybean, five members constitute the SNAP gene family: GmSNAP18, GmSNAP11, GmSNAP14, GmSNAP02, and GmSNAP09. Recently, GmSNAP18 has been reported to mediate resistance to soybean cyst nematode (SCN). Using a population of recombinant inbred lines from resistant and susceptible parents, the divergence of the SNAP gene family is analysed over time. Phylogenetic analysis of SNAP genes from 22 diverse plant species showed that SNAPs were distributed in six monophyletic clades corresponding to the major plant lineages. Conservation of the four TPR motifs in all species, including ancestral lineages, supports the hypothesis that SNAPs were duplicated and derived from a common ancestor and unique gene still present in chlorophytic algae. Syntenic analysis of regions harbouring GmSNAP genes in soybean reveals that this family expanded from segmental and tandem duplications following a tetraploidization event. qRT-PCR analysis of GmSNAPs indicates a co-regulation following SCN infection. Finally, genetic analysis demonstrates that GmSNAP11 contributes to an additive resistance to SCN. Thus, GmSNAP11 is identified as a novel minor gene conferring resistance to SCN.
Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Naoufal
N
Lakhssassi
Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Shiming
S
Liu
Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Sadia
S
Bekal
Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Zhou
Z
Zhou
Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Vincent
V
Colantonio
Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA.
Kris
K
Lambert
Department of Biology, University of South Dakota, Vermillion, SD 57069, USA.
Abdelali
A
Barakat
Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Khalid
K
Meksem
D000818:Animals; D009348:Nematoda / Q000472:pathogenicity; D057865:Plant Immunity / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / Q000737:chemistry / Q000235:genetics*; D013025:Glycine max / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D000074436:Tetratricopeptide Repeat
D010940:Plant Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins