Significance and Function of MicroRNA-7 in Oesophageal Squamous Cell Carcinoma.
We detected microRNA-7 (miR-7) as being specific for oesophageal squamous cell carcinoma (ESCC) by using database analysis. However, the significance of miR-7 in clinical ESCC remains unexplored. This study aimed to clarify the clinicopathological significance of miR-7 in ESCC, and investigate miR-7 function.



Quantitative TaqMan reverse transcription polymerase chain reaction was used to evaluate miR-7 expression in 85 ESCC samples to determine the clinicopathological significance of miR-7 expression. The regulation of proliferation by miR-7 was examined with miR-7 precursor-transfected cells.



The expression of miR-7 in ESCC was higher than that in normal tissues. Low expression levels of miR-7 were associated with poor prognosis. Multivariate analysis indicated that low miR-7 expression was an independent prognostic factor for poor survival. In vitro assays showed miR-7 precursor treatment suppressed the proliferation of ESCC cells.



miR-7 might be a promising prognostic marker and therapeutic target in ESCC.
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Keigo
K
Hara
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan tatsuyamiyazaki@gunma-u.ac.jp.
Tatsuya
T
Miyazaki
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Takehiko
T
Yokobori
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Tomonori
T
Yoshida
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Yuji
Y
Kumakura
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Hiroaki
H
Honjyo
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Makoto
M
Sakai
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Makoto
M
Sohda
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Minoru
M
Fukuchi
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Hiroyuki
H
Kuwano
D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D015999:Multivariate Analysis; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome
D014408:Biomarkers, Tumor; D035683:MicroRNAs; C549242:MIRN7-1 microRNA, human
microRNA; oesophageal cancer; prognostic marker