Stress influenced increase in phenolic content and radical scavenging capacity of Rhodotorula glutinis CCY 20-2-26.
Rhodotorula glutinis CCY 20-2-26 when grown under controlled stress of either NaCl (1-5 %) or H2O2(1-5 mM) on basal media exhibited a twofold increase in its total phenolic contents. The radical scavenging capacities (RSCs) as determined by ABTS test were found to be highest in 4 mM H2O2(1.44 mM TEAC mg-1) and 4 % NaCl (1.13 mM TEAC mg-1) as compared to control samples (0.41 mM TEAC mg-1). Similarly, the RSCs as determined by DPPH test were also highest in 4 % NaCl (1.83 mM TEAC mg-1) and 4 mM H2O2(1.78 mM TEAC mg-1) compared to control (0.48 TEAC mg-1). The relative RSCs from EPR spin-trapping assay for H2O2-stressed cultures were highest in 1 mM H2O2(56.1 μM TEAC g-1) whereas in NaCl-stressed cultures it was highest in 5 % NaCl (44.6 μM TEAC g-1) as compared to control (30.9 μM TEAC g-1). Five phenolic compounds (gallic acid, benzoic acid, catechin, caffeic acid and ferulic acid) were detected for the first time in R. glutinis CCY 20-2-26.
Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, 125 055, India. rajsalar@rediffmail.com.
Raj Kumar
RK
Salar
Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 812 37, Bratislava, Slovak Republic.
Milan
M
Certik
Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 812 37, Bratislava, Slovak Republic.
Vlasta
V
Brezova
Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 812 37, Bratislava, Slovak Republic.
Marta
M
Brlejova
Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 812 37, Bratislava, Slovak Republic.
Vladimira
V
Hanusova
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
Emília
E
Breierová
EPR/spin trapping; HPLC; Phenolics; Radical scavenging capacity; Rhodotorula