Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers.
BACKGROUND
Patients with breast cancer-the deadliest cancer among women-are at constant risk of developing metastasis. Oxidative stress and hypoxia are common feature of tumor cells that can proliferate even in a resultant metabolic acidosis. Despite the low extracellular pH, intracellular pH of tumor cells remains relatively normal, or even more alkaline due to the action of a membrane protein family known as monocarboxylate transporters (MCTs). The objective of this study was to verify the diagnostic and prognostic value ofMCT1,MCT4andCD147in tumor and peripheral blood samples of patients with breast cancer undergoing chemotherapic treatment.


METHODS
Differential expression ofMCT1,MCT4andCD147obtained by qPCR was determined by 2-ΔΔCqmethod between biological samples (tumor and serial samples of peripheral) of patients (n= 125) and healthy women (n= 25).


RESULTS
tumor samples with higher histological grades have shown higher expression of these markers; this higher expression was also observed in blood samples obtained at diagnosis of patients when compared to healthy women and in patients with positive progression of the disease (metastasis development).


CONCLUSIONS
markers studied here could be a promising strategy in routine laboratory evaluations as breast cancer diagnosis and prognosis.
Clinical Laboratory, Faculdade de Medicina do ABC (FMABC), Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil. mb.luz@uol.com.br.
Maria Cláudia de B
MC
Luz
Clinical Laboratory, Faculdade de Medicina do ABC (FMABC), Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil. matheusmpontoperez@gmail.com.
Matheus M
MM
Perez
Biological Science Department, UNIFESP, Rua Prof. Artur Riedel, 275, CEP 09972-270 Diadema, SP, Brazil. laazzalis@uol.com.br.
Ligia A
LA
Azzalis
Epidemiology Laboratory and Data Analysis, FMABC, Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil. luiz.sousa@fmabc.br.
Luiz Vinícius de A
LV
Sousa
Epidemiology Laboratory and Data Analysis, FMABC, Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil. fernando.adami@uol.com.br.
Fernando
F
Adami
Clinical Laboratory, Faculdade de Medicina do ABC (FMABC), Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil. profferfonseca@gmail.com.|Biological Science Department, UNIFESP, Rua Prof. Artur Riedel, 275, CEP 09972-270 Diadema, SP, Brazil. profferfonseca@gmail.com.
Fernando L A
FL
Fonseca
Clinical Laboratory, Faculdade de Medicina do ABC (FMABC), Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil. bcaalves@uol.com.br.
Beatriz da C A
BD
Alves
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood*; D051929:CD146 Antigen / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D027501:Monocarboxylic Acid Transporters / Q000097:blood*; D009124:Muscle Proteins / Q000097:blood*; D027981:Symporters / Q000097:blood*; D000099233:Monocarboxylate Transport Protein 1
D014408:Biomarkers, Tumor; D051929:CD146 Antigen; D027501:Monocarboxylic Acid Transporters; D009124:Muscle Proteins; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C406962:SLC16A4 protein, human
biomarkers; breast cancer; hypoxia; metastasis; monocarboxylate transporter (MCT); prognosis