Helicteric Acid, Oleanic Acid, and Betulinic Acid, Three Triterpenes fromHelicteres angustifoliaL., Inhibit Proliferation and Induce Apoptosis in HT-29 Colorectal Cancer Cells via Suppressing NF-κB and STAT3 Signaling.
Colorectal cancer (CRC) is one of the most common malignancies and most frequent cause of cancer death worldwide. The activation of both NF-κB and STAT3 signaling and the crosstalk between them play an important role in colorectal tumor.Helicteres angustifoliaL. is a type of commonly used Chinese medicinal herb and possesses a wide variety of biological activities. In the present study, we investigate the effects of three triterpenes fromH. angustifolia(HT) such as helicteric acid (HA), oleanic acid (OA), and betulinic acid (BA), on inhibiting CRC progression. Our results showed that HT extracts could decrease proliferation and induce apoptosis in HT-29 colorectal cancer cells. Moreover, HT extracts could suppress LPS-triggered phosphorylation of IKK, IκB, and NF-κB, attenuate IL-6-induced phosphorylation of JAK2 and STAT3, and suppress the expression of c-Myc, cyclin-D1, and BCL-xL, the downstream gene targets of NF-κB and STAT3. Therefore, HT extracts showed potent therapeutic and antitumor effects on CRC via inhibiting NF-κB and STAT3 signaling.
College of Materials Science and Food Engineering, Zhongshan Institute, University of Electronic Science and Technology of China, Zhongshan, Guangdong 528400, China.
Dan
0000-0003-4267-644X
D
Su
The Zhongshan Affiliated Hospital, Guangzhou University of Chinese Medicine, Zhongshan, Guangdong 528401, China.
Yu-Qiao
0000-0001-6391-1379
YQ
Gao
The Zhongshan Affiliated Hospital, Guangzhou University of Chinese Medicine, Zhongshan, Guangdong 528401, China.
Wei-Bo
0000-0002-0719-4026
WB
Dai
The Zhongshan Affiliated Hospital, Guangzhou University of Chinese Medicine, Zhongshan, Guangdong 528401, China.
Ying
0000-0003-1969-5837
Y
Hu
College of Materials Science and Food Engineering, Zhongshan Institute, University of Electronic Science and Technology of China, Zhongshan, Guangdong 528400, China.
Yan-Fen
0000-0002-7002-5620
YF
Wu
The Zhongshan Affiliated Hospital, Guangzhou University of Chinese Medicine, Zhongshan, Guangdong 528401, China.
Quan-Xi
0000-0001-6483-4117
QX
Mei