Investigation of anticarcinogenic and antioxidant effects of -methylimidazole.
4-methylimidazole is widely used in pharmaceuticals, photographic and agricultural chemicals. The substance is extensively found in many human and animals foods. In this research, anticancer effect of the 4-MEI was studded using MTT test using MCF-7 cell line. Effect of the 4-MEI on apoptosis or necrosis was analyzed by DNA fragmentation assay using Swiss Albino rats as a model organism. Antioxidant effect of the substance was investigated by assaying protective effect of the substance on circular plasmid DNA against H2O2 as an oxidative agent. 4-MEI showed inhibitory effect on proliferation of MCF-7 cell line by all concentrations and the decrement was significant and concentration dependent. Result of DNA fragmentation assay showed 4-MEI concentrations dependent of smear formation showing necrotic effects of the 4-MEI on mouse cells. Also, the 4-MEI showed a good antioxidant activity and protective effect against H2O2.



The result of this study showed that 4MEI has significant antioxidant and anti-cancer effect. Also, according to the result, 4-MEI has necrotic effects on mouse cells (Fig. 3, Ref. 21).
M Norizadeh
MN
Tazehkand
O
O
Hajipour
F
F
Moridikia
A
A
Moridikia
E
E
Valipour
M B
MB
Yilmaz
M
M
Topaktas
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D061986:MCF-7 Cells; D051379:Mice; D009336:Necrosis; D016877:Oxidants / Q000494:pharmacology*; D051381:Rats
D000975:Antioxidants; D007093:Imidazoles; D016877:Oxidants; D006861:Hydrogen Peroxide; C023439:4-methylimidazole
4-MEI; MCF-7; antioxidant anticancer.; fragmentation