Peptide-protein interactions within human hair keratins.
We selected 1235 decapeptides from human hair proteins encoded by human genes of keratins and keratin associated proteins. The peptides were linked to glass arrays and screened for their affinity towards a solution of human hair extracted keratin fraction. Based on the physicochemical properties of the peptides, ten variables were studied: content of different types of amino acid side chains (cysteine, hydrophobic, polar, basic, acidic, aromatic rings, amide, alcohol side chains), isoelectric point, and net charge. We found differences statistically significant on the binding affinity of peptides based on their content of cysteine, hydrophobic and polar amino acids, mainly containing alcohols. These results point to the formation of hydrophobic interactions and disulfide bonds between small peptides and human hair keratins as the main driving forces for the interaction of possible cosmetic peptides, namely designed to strength human hair. As so, our results enlighten the nature of the interaction of keratin based materials with human hair, which are claimed to enhance hair fiber strength, and enable a more directed and sustained hair care peptide design.
CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Célia F
CF
Cruz
CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Nuno G
NG
Azoia
CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Teresa
T
Matamá
CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. Electronic address: artur@deb.uminho.pt.
Artur
A
Cavaco-Paulo
D006801:Humans; D053564:Keratins, Hair-Specific / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D040081:Protein Array Analysis; D011485:Protein Binding
D053564:Keratins, Hair-Specific; D010446:Peptide Fragments
Cosmetic peptide; Cysteine; Hair keratin; Hydrophobicity; Peptide microarray