Protective Effect of Lactobacillus casei on DMH-Induced Colon Carcinogenesis in Mice.
The administration of probiotics is a promising approach to reduce the prevalence of colon cancer, a multifactorial disease, with hereditary factors, as well as environmental lifestyle-related risk factors. Biogenic polyamines, putrescine, spermidine, and spermine are small cationic molecules with great roles in cell proliferation and differentiation as well as regulation of gene expression. Ornithine decarboxylase is the first rate-limiting enzyme for polyamine synthesis, and upregulation of ornithine decarboxylase activity and polyamine metabolism has been associated with abnormal cell proliferation. This paper is focused on studying the protective role of Lactobacillus casei ATCC 393 in a chemically induced mouse model of colon carcinogenesis, directing our attention on aberrant crypt foci as preneoplastic markers, and on polyamine metabolism as a possible key player in carcinogenesis. BALB/c mice were administered 1,2-dimethylhydrazine dihydrochloride (DMH) to induce colon cancer (20 mg/kg body weight, subcutaneous, twice a week for 24 weeks). L. casei ATCC 393 was given orally (106CFU, twice a week), 2 weeks before DMH administration. Hematoxylin and eosin staining, high-performance liquid chromatography, and Western blotting were used to evaluate aberrant crypt foci, urinary polyamines, and ornithine decarboxylase expression in the colon. The experimental data showed that the preventive administration of L. casei ATCC 393 may delay the onset of cancer as it significantly reduced the number of DMH-induced aberrant crypt foci, the levels of putrescine, and the expression of ornithine decarboxylase. Hence, this probiotic strain has a prospective role in protection against colon carcinogenesis, and its antimutagenic activity may be associated with the maintenance of polyamine metabolism.
Departamento de Salud, El Colegio de La Frontera Sur, Periférico Sur s/n, María Auxiliadora, 29290, San Cristóbal de Las Casas, Chiapas, Mexico.
Cesar Antonio
CA
Irecta-Nájera
Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Carretera Guadalajara-Nogales Km 15.5, Las Agujas, C.P. 45110, Zapopan, Jalisco, Mexico.
María
M
Del Rosario Huizar-López
Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Carretera Guadalajara-Nogales Km 15.5, Las Agujas, C.P. 45110, Zapopan, Jalisco, Mexico.
Josefina
J
Casas-Solís
Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Carretera Guadalajara-Nogales Km 15.5, Las Agujas, C.P. 45110, Zapopan, Jalisco, Mexico.
Patricia
P
Castro-Félix
Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Carretera Guadalajara-Nogales Km 15.5, Las Agujas, C.P. 45110, Zapopan, Jalisco, Mexico. asanterre@live.com.mx.
Anne
0000-0003-2460-9630
A
Santerre
D000818:Animals; D063646:Carcinogenesis; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D003110:Colonic Neoplasms / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D004127:Dimethylhydrazines / Q000633:toxicity*; D005260:Female; D006801:Humans; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000502:physiology*; D051379:Mice; D011073:Polyamines / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage*
D004127:Dimethylhydrazines; D011073:Polyamines
1,2-Dimethylhydrazine; Aberrant crypt foci; Colon carcinogenesis; Lactobacillus casei; Ornithine decarboxylase; Putrescine