Production of reuterin in a fermented milk product by Lactobacillus reuteri: Inhibition of pathogens, spoilage microorganisms, and lactic acid bacteria.
We assessed the antimicrobial activity of reuterin produced in vitro in glycerol aqueous solutions in situ by Lactobacillus reuteri ATCC 53608 as part of a fermented milk product against starter (Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus), spoilage (Penicillium expansum), pathogenic (Staphylococcus aureus Salmonella enterica ssp. enterica, and Listeria monocytogenes), and pathogen surrogate (Escherichia coli DH5α) microorganisms. We also assayed the influence of cold storage (28 d at 4°C) and reuterin on the color and rheology of the fermented milk product. We obtained maximum reuterin concentrations of 107.5 and 33.97 mM in glycerol aqueous solution and fermented milk product, respectively. Reuterin was stable throughout its refrigerated shelf life. Gram-positive microorganisms were more resistant to reuterin than gram-negative microorganisms. Penicillium expansum and Lactobacillus reuteri ATCC 53608 survived at concentrations up to 10 and 8.5 mM, respectively. Escherichia coli DH5α was the most sensitive to reuterin (0.9 mM). The presence of reuterin did not cause relevant changes in the quality parameters of the fermented milk product, including pH, acidity, soluble solids, color, and rheological aspects (storage and loss moduli and viscosity). This study demonstrated the viability of using Lactobacillus reuteri ATCC 53608 as a biopreservative in a fermented milk product through reuterin synthesis, without drastically modifying its quality parameters.
Centro de Investigación en Alimentación y Desarrollo A.C., Unidad Cuauhtémoc, Av. Río Conchos S/N, Parque Industrial, Ciudad Cuauhtémoc, Chihuahua, C.P. 31570, México.
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Ortiz-Rivera
Centro de Investigación en Alimentación y Desarrollo A.C., Unidad Cuauhtémoc, Av. Río Conchos S/N, Parque Industrial, Ciudad Cuauhtémoc, Chihuahua, C.P. 31570, México.
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Sánchez-Vega
Universidad Autónoma de Chihuahua, Facultad de Ciencias Químicas, Campus Universitario #2, Circuito Universitario Chihuahua, C.P. 31125, México.
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Gutiérrez-Méndez
Centro de Investigación en Alimentación y Desarrollo A.C., Unidad Culiacán, Carretera a El Dorado, Km 5.5, Campo el 10, Culiacán, Sinaloa, C.P. 80129, México.
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León-Félix
Centro de Investigación en Alimentación y Desarrollo A.C., Unidad Cuauhtémoc, Av. Río Conchos S/N, Parque Industrial, Ciudad Cuauhtémoc, Chihuahua, C.P. 31570, México.
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Acosta-Muñiz
Centro de Investigación en Alimentación y Desarrollo A.C., Unidad Cuauhtémoc, Av. Río Conchos S/N, Parque Industrial, Ciudad Cuauhtémoc, Chihuahua, C.P. 31570, México. Electronic address: dsepulveda@ciad.mx.
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Sepulveda
D000818:Animals; D043302:Cultured Milk Products / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects; D061353:Food Storage / Q000379:methods; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D019344:Lactic Acid; D048668:Lactobacillus delbrueckii / Q000187:drug effects; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D010407:Penicillium / Q000187:drug effects; D011407:Propane / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D012034:Refrigeration; D019779:Salmonella enterica / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D048249:Streptococcus thermophilus / Q000187:drug effects
C047158:3-hydroxypropionaldehyde; D019344:Lactic Acid; D005985:Glyceraldehyde; D011407:Propane
Lactobacillus reuteri; biopreservation; fermented milk product; reuterin