A Novel Soybean ERF Transcription Factor,GmERF113, Increases Resistance toPhytophthora sojaeInfection in Soybean.
Phytophthora root and stem rot of soybean caused by the oomycetePhytophthora sojae, is a destructive disease worldwide. Ethylene response factors (ERFs) play important roles in regulating plant biotic and abiotic stress tolerance. In this study, a new ERF gene,GmERF113, was isolated from the highly resistant soybean 'Suinong 10.' Sequence analysis suggested that the protein encoded byGmERF113contained a conserved AP2/ERF domain of 58 amino acid and belonged to the B-4 subgroup of the ERF subfamily. Expression ofGmERF113was significantly induced byP. sojae, ethylene, and methyl jasmonate. GmERF113 protein localized to the nucleus when transiently expressed inArabidopsisprotoplasts, could bind to the GCC-box, and acted as a transcription activator. In addition, a region of the full-length GmERF113, GmERF113-II, interacted with a basic helix-loop-helix transcription factor (GmbHLH) in yeast cells. Full-length GmERF113 also interacted with GmbHLHin planta. GmERF113-overexpressing transgenic plants in susceptible cultivar 'Dongnong 50' soybean exhibited increased resistance toP. sojaeand positively regulated the expression of the pathogenesis-related genes,PR1andPR10-1. These results indicate thatGmERF113may play a crucial role in the defense of soybean againstP. sojaeinfection.
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural UniversityHarbin, China; Biotechnology Research Institute, Heilongjiang Academy of Agricultural SciencesHarbin, China.
Yuanling
Y
Zhao
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Xin
X
Chang
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Dongyue
D
Qi
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Lidong
L
Dong
Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences Harbin, China.
Guangjin
G
Wang
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Sujie
S
Fan
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Liangyu
L
Jiang
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Qun
Q
Cheng
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Xi
X
Chen
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Dan
D
Han
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Pengfei
P
Xu
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University Harbin, China.
Shuzhen
S
Zhang
AP2/ERF; Glycine max; GmERF113; Phytophthora sojae; activator