An Anti-Programmed Death-1 Antibody (αPD-1) Fusion Protein That Self-Assembles into a Multivalent and Functional αPD-1 Nanoparticle.
Cancer immune checkpoint therapy has achieved remarkable clinical successes in various cancers. However, current immune checkpoint inhibitors block the checkpoint of not only the immune cells that are important to cancer therapy but also the immune cells that are irrelevant to the therapy. Such an indiscriminate blockade limits the efficacy and causes the autoimmune toxicity of the therapy. It might be beneficial to use a carrier to target immune checkpoint inhibitors to cancer-reactive immune cells. Here, we explore a method to load the inhibitors into carriers. We used the anti-programmed death-1 antibody (αPD-1) as a model immune checkpoint inhibitor. First, we generated a recombinant single-chain variable fragment (scFv) of αPD-1. Then, we designed and generated a fusion protein consisting of the scFv and an amphiphilic immune-tolerant elastin-like polypeptide (iTEP). Because of the amphiphilic iTEP, the fusion was able to self-assemble into a nanoparticle (NP). The NP was proved to block the PD-1 immune checkpoint in vitro and in vivo. Particularly, the NP exacerbated diabetes development in nonobese diabetic mice as effectively as natural, intact αPD-1. In summary, we successfully expressed αPD-1 as a recombinant protein and linked αPD-1 to a NP, which lays a foundation to develop a delivery system to target αPD-1 to a subpopulation of immune cells.
Department of Pharmaceutics and Pharmaceutical Chemistry, The University of Utah , Salt Lake City, Utah 84112, United States.
Peng
P
Zhao
University of Utah School of Medicine , Salt Lake City, Utah 84112, United States.
Djordje
D
Atanackovic
Department of Pharmaceutics and Pharmaceutical Chemistry, The University of Utah , Salt Lake City, Utah 84112, United States.
Shuyun
S
Dong
Department of Immunology, Juntendo University School of Medicine , Tokyo, Japan.
Hideo
H
Yagita
Department of Pathology, The University of Utah , Salt Lake City, Utah 84112, United States.
Xiao
X
He
Department of Pharmaceutics and Pharmaceutical Chemistry, The University of Utah , Salt Lake City, Utah 84112, United States.
Mingnan
0000-0002-7080-9804
M
Chen
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D045744:Cell Line, Tumor; D000067493:Dynamic Light Scattering; D057446:Hydrodynamics; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*
D000906:Antibodies
anti-programmed death-1 antibody; diabetes; multivalency; nanoparticle; recombinant anti-programmed death-1 antibody fusion