A Novel Water-Soluble Fluorescence Probe with Wash-Free Cellular Imaging Capacity Based on AIE Characteristics.
A potential real-time imaging water-soluble fluorescent polymer (P3) is facilely prepared via one-pot method. For P3, tetraphenylethene unit serves as the fluorescent unit, poly(acryloyl ethylene diamine) (a kind of polyelectrolyte) with specific degree of polymerization acts as water-soluble part.1H-NMR, gel permeation chromatography (GPC), UV-vis spectroscopy, photoluminescence (PL), and confocal laser scanning microscopy are undertaken to characterize the structure and property of P3. The results of wash-free cellular imaging show that the signal-to-noise ratio is high as the concentration of P3 is 50 μg mL-1. In addition, the pH-responsive and Cd2+-responsive are also investigated in this paper. The results coming from pH-responsive show that P3 solution displays significant fluorescence under near neutral. And the result from the cellular imaging shows that intracellular fluorescence intensity enhances with the augment of concentration of Cd2+, which reveals that P3 can give a hint to resolve the dilemma of traditional fluorescent dyes used as living cellular fluorescent probe.
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
Yunxia
Y
Qian
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Baoding, 071002, P. R. China.
Hongmei
H
Liu
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
Haijian
H
Tan
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
Qingmin
Q
Yang
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Baoding, 071002, P. R. China.
Shuchen
S
Zhang
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
Lingui
L
Han
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
Xuegang
X
Yi
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Baoding, 071002, P. R. China.
Li
L
Huo
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Baoding, 071002, P. R. China.
Hongchi
H
Zhao
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Baoding, 071002, P. R. China.
Yonggang
Y
Wu
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Baoding, 071002, P. R. China.
Libin
L
Bai
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Baoding, 071002, P. R. China.
Xinwu
X
Ba
D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D011108:Polymers; D014867:Water
aggregation-induced emission; pH-induced fluorescence response; tetraphenylethene; wash-free cellular imaging; water-soluble fluorescent polymer