Tmeff2 is expressed in differentiating oligodendrocytes but dispensable for their differentiation in vivo.
Myelin elaborated by oligodendrocytes (OLs) in the central nervous system (CNS) is required for saltatory conduction of action potentials along neuronal axons. We found that TMEFF2, a transmembrane protein with EGF-like and two follistatin-like domains, is selectively expressed in differentiating/myelinating OLs. Previous studies showed that TMEFF2 is capable of binding to PDGFA, which plays important roles in the proliferation, migration and differentiation of oligodendrocyte progenitor cells (OPCs). However, molecular and genetic analysis revealed that Tmeff2 is a weak binder of PDGFA, and not required for OL differentiation and myelin gene expression in vivo. Together, our data suggested that Tmeff2 is specifically upregulated in OLs, but dispensable for OL differentiation and maturation.
The College of Life Sciences, Zhejiang University, Hangzhou, 310036, China.|Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.|Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY, 40292, USA.
Hao
H
Huang
The College of Life Sciences, Zhejiang University, Hangzhou, 310036, China.|Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Peng
P
Teng
Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Ruyi
R
Mei
Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Aifen
A
Yang
Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Zunyi
Z
Zhang
Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China. xiaofengzhao@yahoo.com.
Xiaofeng
X
Zhao
The College of Life Sciences, Zhejiang University, Hangzhou, 310036, China. m0qiu001@yahoo.com.|Institute of Life Sciences, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China. m0qiu001@yahoo.com.|Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY, 40292, USA. m0qiu001@yahoo.com.
Mengsheng
M
Qiu
D000818:Animals; D002454:Cell Differentiation; D020869:Gene Expression Profiling; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D018345:Mice, Knockout; D009836:Oligodendroglia / Q000502:physiology*
D008565:Membrane Proteins; C412455:Tmeff2 protein, mouse