Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout.
Rainbow trout (Oncorhynchus mykiss) is considered a "glucose-intolerant" species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L-1O2) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3. At first-feeding, trout previously subjected to the 2.5 mg·L-1O2hypoxia displayed a strong induction of glycolytic and glucose transport genes, whereas these glucose metabolism-related genes were affected much less in trout subjected to the less severe (5.0 mg·L-1O2) hypoxia. Our results demonstrate that an acute hypoxic stimulus during early development can affect glucose metabolism in trout at first-feeding.
INRA, Univ Pau & Pays de l'Adour, UMR1419 Nutrition Metabolism and Aquaculture, F-64310, Saint Pée sur Nivelle, France.
Jingwei
J
Liu
INRA, Univ Pau & Pays de l'Adour, UMR1419 Nutrition Metabolism and Aquaculture, F-64310, Saint Pée sur Nivelle, France.
Elisabeth
E
Plagnes-Juan
INRA, Univ Pau & Pays de l'Adour, UMR1419 Nutrition Metabolism and Aquaculture, F-64310, Saint Pée sur Nivelle, France.
Inge
I
Geurden
INRA, Univ Pau & Pays de l'Adour, UMR1419 Nutrition Metabolism and Aquaculture, F-64310, Saint Pée sur Nivelle, France.
Stéphane
S
Panserat
INRA, Univ Pau & Pays de l'Adour, UMR1419 Nutrition Metabolism and Aquaculture, F-64310, Saint Pée sur Nivelle, France. lucie.marandel@inra.fr.
Lucie
L
Marandel
D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000378:metabolism; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D006019:Glycolysis; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017686:Oncorhynchus mykiss / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D029941:Fish Proteins; D012333:RNA, Messenger; D005947:Glucose