Lactic acid production from submerged fermentation of broken rice using undefined mixed culture.
The present work aimed to characterize and optimize the submerged fermentation of broken rice for lactic acid (LA) production using undefined mixed culture from dewatered activated sludge. A microorganism with amylolytic activity, which also produces LA, Lactobacillus amylovorus, was used as a control to assess the extent of mixed culture on LA yield. Three level full factorial designs were performed to optimize and define the influence of fermentation temperature (20-50 °C), gelatinization time (30-60 min) and broken rice concentration in culture medium (40-80 g L-1) on LA production in pure and undefined mixed culture. LA production in mixed culture (9.76 g L-1) increased in sixfold respect to pure culture in optimal assessed experimental conditions. The optimal conditions for maximizing LA yield in mixed culture bioprocess were 31 °C temperature, 45 min gelatinization time and 79 g L-1broken rice concentration in culture medium. This study demonstrated the positive effect of undefined mixed culture from dewatered activated sludge to produce LA from culture medium formulated with broken rice. In addition, this work establishes the basis for an efficient and low-cost bioprocess to manufacture LA from this booming agro-industrial by-product.
Grupo de Engenharia de Bioprocessos, Departamento de Ciências Biológicas, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Assis, Avenida Dom Antônio, 2100, Assis, SP, 19806-900, Brazil.
Luiza Varela
LV
Nunes
Laboratório de Biotecnologia Industrial, Departamento de Biotecnologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Assis, Avenida Dom Antônio, 2100, Assis, SP, 19806-900, Brazil.
Fabiane Fernanda
FF
de Barros Correa
Laboratório de Biotecnologia Industrial, Departamento de Biotecnologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Assis, Avenida Dom Antônio, 2100, Assis, SP, 19806-900, Brazil.
Pedro
P
de Oliva Neto
Laboratório de Química de Alimentos e Nanobiotecnologia, Departamento de Biotecnologia, Universidade Estadual Paulista "Júlio de Mesquita Filho", Campus-Assis, Avenida Dom Antonio 2100, Bairro Parque Universitário, Assis, SP, 19806-900, Brazil.
Cassia Roberta Malacrida
CR
Mayer
Laboratório de Biotecnologia Industrial, Departamento de Biotecnologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Assis, Avenida Dom Antônio, 2100, Assis, SP, 19806-900, Brazil.
Bruna
B
Escaramboni
Laboratório de Biotecnologia Industrial, Departamento de Biotecnologia, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Assis, Avenida Dom Antônio, 2100, Assis, SP, 19806-900, Brazil.
Tania Sila
TS
Campioni
Instituo de Química - Araraquara, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Araraquara, Rua Professor Francisco Degni, 55, Araraquara, SP, 14800-900, Brazil.
Natan Roberto
NR
de Barros
Instituo de Química - Araraquara, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Araraquara, Rua Professor Francisco Degni, 55, Araraquara, SP, 14800-900, Brazil.
Rondinelli Donizetti
RD
Herculano
Grupo de Engenharia de Bioprocessos, Departamento de Ciências Biológicas, Universidade Estadual Paulista 'Júlio de Mesquita Filho' Campus-Assis, Avenida Dom Antônio, 2100, Assis, SP, 19806-900, Brazil. eutimiocu@yahoo.com.|Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Avenida dos Estados, 5001, Santo André, SP, 09210-580, Brazil. eutimiocu@yahoo.com.
Eutimio Gustavo
EG
Fernández Núñez
D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry*; D012722:Sewage / Q000382:microbiology*; D013696:Temperature
D003470:Culture Media; D012722:Sewage; D019344:Lactic Acid
Activated sludge; Broken rice; Lactic acid; Lactobacillus amylovorus; Mixed culture; Three-level factorial design