Statistical evaluation of the medium components for the production of high biomass, α-amylase and protease enzymes by Piriformospora indica using Plackett-Burman experimental design.
Piriformospora indica, a member of basidiomycota is an axenically cultivable endophytic fungus which exerts plant growth promoting effects on its host plant. P. indica is known to produce α-amylase and protease. Since the organism exhibits beneficial role in plant growth promotion, achieving high biomass is immensely essential. Hence to enable the commercial production, screening of medium components is a necessary step. The present paper investigates the screening of medium components using Plackett-Burman experimental design wherein the parameters such as α-amylase, protease and biomass have been examined. The parameters α-amylase, protease and biomass was found to vary from 0.25 to 0.45 mg-1ml-1min-1, 0.1 to 0.15 mg-1ml-1h-1and 0.8 to 22.6 g l-1, respectively, in 16 runs which demonstrates the strong influence of the medium components.
Department of Microbiology, Centre of PG Studies, Jain University, 9th Main, Jayanagar 3rd Block, Bangalore, India.
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Swetha
Amity Institute of Herbal and Microbial Studies, Sector 125, Noida, 201303, UP, India.
Ajit
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Varma
Department of Microbiology, Centre of PG Studies, Jain University, 9th Main, Jayanagar 3rd Block, Bangalore, India. vam2010tpraviju@gmail.com.
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Padmavathi
Piriformospora indica; Plackett–Burman experimental design; Protease; Submerged fermentation; α-Amylase