Biomonitored UHPLC-ESI-QTOF-MS2and HPLC-UV thermostability study of the aerial parts of Sphagneticola trilobata (L.) Pruski, Asteraceae.
As the temperature of extraction and processing could impact the biological effects of herbal extracts, which have wide chemical diversity, the aim of this work were to investigate the thermostability of herbal derivatives of the aerial parts of Sphagneticola trilobata, a reputed medicinal plant; to biomonitor its oral anti-hyperalgesic effect; and to elucidate the degradation pathways of the major components by UHPLC-ESI-QTOF-MS. The lipophilic markers (kaurenoic acid-KA) and hydrophilic markers [chlorogenic acids; measured as total phenolic compounds (PC), expressed in caffeic acid] were also monitored through a validated HPLC-UV methodology, suitable for quality control and stability studies. The soft extract (drug:solvent ratio of 1:10, ethanol 60% v/v) was exposed to high temperatures (50-180°C). PC showed high thermolability (27.4% of degradation at 150°C), compared to KA (16.5%, at 180°C). These results suggest that the loss of oral anti-hyperalgesic activity observed in the spray-dried extracts (dried at 170°C), compared with the soft and the lyophilized extract may be related to degradation of the active components, especially the polar compounds, i.e. chlorogenic acid derivatives and the four oleanane type triterpenoid saponins. These latter degraded at temperatures above 150°C and vanished at 180°C, as demonstrated in the UHPLC-ESI-QTOF-MS analyses. These results provide a relevant guide for the extraction process of S. trilobata, aimed at preserving the antinociceptive effect.
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Keline
K
Lang
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Jessica
J
Corrêa
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Fellippe
F
Wolff
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Gislaine Franciele
GF
da Silva
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Angela
A
Malheiros
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Valdir Cechinel
VC
Filho
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Ruth Meri Lucinda
RML
Silva
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Nara Lins Meira
NLM
Quintão
Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis 88040-900, SC, Brazil.
Louis P
LP
Sandjo
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil.
Tiago J
TJ
Bonomini
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Program of Post-Graduation in Pharmaceutical Sciences, Universidade do Vale do Itajaí (UNIVALI), Caixa postal 360, CEP 88302-202 Itajaí, SC, Brazil. Electronic address: tbresolin@univali.br.
Tania Mari Bellé
TMB
Bresolin
D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D014466:Ultraviolet Rays
D010936:Plant Extracts
HPLC-UV; Lipophilic and hydrophilic markers; Oral antinociception activity; Sphagneticola trilobata; Thermostability; UHPLC-ESI-QTOF-MS