Enzootic bovine leukosis in a two-month-old calf.
A two-month-old calf was diagnosed with leukosis on the basis of the clinical sign of enlarged, superficial lymph nodes. Serological and genetic tests for bovine leukemia virus (BLV) were performed because the calf was born from a cow infected with BLV. The serum had a weakly positive BLV antibody, and the BLV provirus was detected within neoplastic cells on performing polymerase chain reaction (PCR). Analysis of the BLV provirus integration site using inverse PCR revealed that the BLV integration site location was identical on all chromosomes in all tumor tissues examined. Thus, the tumor cells monoclonally proliferated following BLV infection. The present study shows that enzootic bovine leukosis can occur in a young animal, as in the two-month-old calf in our study.
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
Keisuke
K
Oguma
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
Miho
M
Suzuki
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan. Electronic address: sentsui.hiroshi@nihon-u.ac.jp.
Hiroshi
H
Sentsui
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002417:Cattle; D049109:Cell Proliferation; D002999:Clone Cells; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis / Q000473:pathology / Q000821:virology*; D018445:Infectious Disease Transmission, Vertical; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D007962:Leukocytes / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology*; D008297:Male; D011533:Proviruses / Q000235:genetics / Q000378:metabolism; D016662:Virus Integration
D000914:Antibodies, Viral; D004279:DNA, Viral
BLV; Calf; EBL; Integration site; Inverse PCR