Magnitude-dependent response of osteoblasts regulated by compressive stress.
The present study aimed to investigate the role of magnitude in adaptive response of osteoblasts exposed to compressive stress. Murine primary osteoblasts and MC3T3-E1 cells were exposed to compressive stress (0, 1, 2, 3, 4, and 5 g/cm2) in 3D culture. Cell viability was evaluated, and expression levels of Runx2, Alp, Ocn, Rankl, and Opg were examined. ALP activity in osteoblasts and TRAP activity in RAW264.7 cells co-cultured with MC3T3-E1 cells were assayed. Results showed that compressive stress within 5.0 g/cm2did not influence cell viability. Both osteoblastic and osteoblast-regulated osteoclastic differentiation were enhanced at 2 g/cm2. An increase in stress above 2 g/cm2did not enhance osteoblastic differentiation further but significantly inhibited osteoblast-regualted osteoclastic differentiation. This study suggested that compressive stress regulates osteoblastic and osteoclastic differentiation through osteoblasts in a magnitude-dependent manner.
Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.|School of Dentistry, University of Detroit Mercy, Detroit, Michigan, USA.
Xiao-Qing
XQ
Shen
Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Yuan-Ming
YM
Geng
Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, China.
Ping
P
Liu
Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, China.
Xiang-Yu
XY
Huang
Key laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China.
Shu-Yi
SY
Li
Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Chun-Dong
CD
Liu
School of Dentistry, University of Detroit Mercy, Detroit, Michigan, USA.
Zheng
Z
Zhou
Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, China.
Ping-Ping
PP
Xu
D016475:3T3 Cells; D000222:Adaptation, Physiological; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D018874:Spheroids, Cellular; D013314:Stress, Mechanical