Imaging of Brain Slices with a Genetically Encoded Voltage Indicator.
Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.
Department of Bioengineering, Imperial College London, South Kensington, London, SW7 2AZ, UK.|Centre for Neurotechnology, Imperial College London, South Kensington, London, SW7 2AZ, UK.|Division of Brain Sciences, Imperial College London, Hammersmith Campus, London, W12 0NN, UK.
Peter
P
Quicke
Division of Brain Sciences, Imperial College London, Hammersmith Campus, London, W12 0NN, UK.
Samuel J
SJ
Barnes
Centre for Neurotechnology, Imperial College London, South Kensington, London, SW7 2AZ, UK. tknopfel@knopfel-lab.net.|Division of Brain Sciences, Imperial College London, Hammersmith Campus, London, W12 0NN, UK. tknopfel@knopfel-lab.net.
Thomas
T
Knöpfel
D001921:Brain / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D056969:Voltage-Sensitive Dye Imaging / Q000379:methods*
D008164:Luminescent Proteins
Brain slices; Fluorescence imaging; Fluorescence microscopy; Voltage imaging; Voltage indicators; Voltage sensitive fluorescent proteins