Trans Fatty Acids Suppress TNF-α-Induced Inflammatory Gene Expression in Endothelial (HUVEC) and Hepatocellular Carcinoma (HepG2) Cells.
Trans fatty acids (TFA) intake has been linked to cardiovascular diseases and liver diseases; yet the effect of TFA on inflammation remains controversial. Accordingly, the objective of this paper was to determine the in vitro effects of TFA on inflammatory gene expression. Human umbilical vein endothelial cells (HUVEC) and human hepatocellular carcinoma (HepG2) cells were treated for 24 h with either trans-vaccenic acid (tVA), trans-palmitoleic acid (tPA) or elaidic acid (EA) at concentrations of 5-150 µM, or with a mixture of tVA and tPA (150/50 µM). All TFA were highly incorporated into cell membranes, as determined by gas chromatography, representing 15-20% of total fatty acids in HUVEC and 3-8% in HepG2 cells. Incorporation of EA, a common industrial TFA, increased the ratio of the stearoyl-CoA desaturase (SCD-1), a key enzyme involved in fatty acid metabolism. Ruminant TFA, including tVA, tPA and the mixture of tVA and tPA, significantly reduced the TNF-α-induced gene expression of TNF, VCAM-1 and SOD2 in HUVEC, as well as TNF and IL-8 in HepG2 cells. EA also decreased inflammatory gene expression in HUVEC, but not in HepG2 cells. The inhibition of peroxisome proliferator-activated receptor (PPAR)-γ did not influence the effects of TFA on gene expression. Overall, physiological and supraphysiological concentrations of TFA, especially tVA and tPA, prevented inflammatory gene expression in vitro. This effect is independent of PPAR-γ activation and may be due to an alteration of fatty acid metabolism in cell membranes caused by the high incorporation of TFA.
Endocrinology and Nephrology Unit, Centre de recherche du CHU de Québec, Université Laval, 2705, boul. Laurier, Québec City, QC, G1V 4G2, Canada.|The Department of Kinesiology, Faculty of Medicine, Université Laval, 2325 rue de l'Université, Québec City, QC, G1V 0A6, Canada.
Marine S
MS
Da Silva
Endocrinology and Nephrology Unit, Centre de recherche du CHU de Québec, Université Laval, 2705, boul. Laurier, Québec City, QC, G1V 4G2, Canada.|The Department of Medicine, Faculty of Medicine, Université Laval, 2325 rue de l'Université, Québec City, QC, G1V 0A6, Canada.
Pierre
P
Julien
Endocrinology and Nephrology Unit, Centre de recherche du CHU de Québec, Université Laval, 2705, boul. Laurier, Québec City, QC, G1V 4G2, Canada.|The Department of Medicine, Faculty of Medicine, Université Laval, 2325 rue de l'Université, Québec City, QC, G1V 0A6, Canada.
Jean-François
JF
Bilodeau
Laboratory of Molecular Pharmacology, Centre de recherche du CHU de Québec, Université Laval, 2705, boul. Laurier, Québec City, QC, G1V 4G2, Canada.|The Faculty of Pharmacy, Université Laval, 2325 rue de l'Université, Québec City, QC, G1V 0A6, Canada.
Olivier
O
Barbier
Endocrinology and Nephrology Unit, Centre de recherche du CHU de Québec, Université Laval, 2705, boul. Laurier, Québec City, QC, G1V 4G2, Canada. iwona.rudkowska@crchudequebec.ulaval.ca.|The Department of Kinesiology, Faculty of Medicine, Université Laval, 2325 rue de l'Université, Québec City, QC, G1V 0A6, Canada. iwona.rudkowska@crchudequebec.ulaval.ca.
Iwona
I
Rudkowska
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D056945:Hep G2 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D050356:Lipid Metabolism / Q000187:drug effects; D008113:Liver Neoplasms / Q000276:immunology*; D013230:Stearoyl-CoA Desaturase / Q000235:genetics; D044242:Trans Fatty Acids; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; C508606:CXCL8 protein, human; D016209:Interleukin-8; C439524:TNF protein, human; D044242:Trans Fatty Acids; D014409:Tumor Necrosis Factor-alpha; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase
Endothelial cells; Gene expression; HepG2; Inflammation; Trans fatty acids