A Rapid Approach for Fabricating Boronic Acid-Functionalized Plates for On-Probe Detection of Glycoprotein and Glycopeptide.
We developed a rapid and simple approach without using complex mechanical or chemical protocols to fabricate boronic acid-functionalized plates for glycoprotein or glycopeptide enrichment and mass spectrometry (MS) analysis. By coating the boronic acid-functionalized silica particles on a polydimethylsiloxane (PDMS)-coated matrix-assisted laser desorption/ionization (MALDI) plate, these particles can form a firmly monolayer of particles on PDMS membrane for sample handling without peeling off. The boronic acid particles-coated PDMS plate (BP plate) was successfully applied to the enrichment of horseradish peroxidase (HRP) protein and their digested glycopeptides.
Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital.
Yu-Ching
YC
Liu
Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital; Graduate Institute of Integrated Medicine, China Medical University.
Chao-Jung
CJ
Chen
MALDI-TOF; boronic acid; glycoprotein; on-probe