Densoviruses in oyster Crassostrea ariakensis.
Densoviruses have short ssDNA genomes and mainly infect arthropods. To characterize viral nucleic acid in shellfish, oysters (Crassostrea ariakensis) were analyzed using viral metagenomics. Two large de novo assembled contigs, CaaDV1 and CaaDV2, consisting of nearly complete densovirus genomes (5860 nucleotides (nt) and 4034 nt) with two major ambisense protein coding regions were identified. Several potential non-structural proteins and capsid proteins were encoded by these genomes, but these were divergent from the existing densoviral species. The NS1 protein of the two CaaDVs shared 43.3%~61.5% amino acid identities with the sea star-associated densovirus and cherax quadricarinatus densovirus, with the four species clustering by phylogenetic analysis. This is the first report of densovirus detection in shellfish, increasing the potential host range of densoviruses and the genetic diversity of the genus Ambidensovirus.
Wuxi School of Medicine, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People's Republic of China.|Public Health Research Center, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People's Republic of China.
Yan-Jun
YJ
Kang
Wuxi School of Medicine, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People's Republic of China.
Wei
W
Huang
Wuxi School of Medicine, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People's Republic of China.
A-Li
AL
Zhao
Wuxi School of Medicine, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People's Republic of China.
Dan-Dan
DD
Lai
Affiliated Hospital of Jiangnan University, Huihe Road 200, Wuxi, 214062, Jiangsu, People's Republic of China.
Lin
L
Shao
Wuxi School of Medicine, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People's Republic of China.|Public Health Research Center, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People's Republic of China.
Yan-Qin
YQ
Shen
Department of Laboratory Medicine, Blood Systems Research Institute, University of California San Francisco, San Francisco, CA, USA.
Xutao
X
Deng
Department of Laboratory Medicine, Blood Systems Research Institute, University of California San Francisco, San Francisco, CA, USA.
Eric
E
Delwart
School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 202103, Jiangsu, People's Republic of China. z0216wen@yahoo.com.
Wen
W
Zhang
D000818:Animals; D049896:Crassostrea / Q000821:virology*; D017990:Densovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D056186:Metagenomics; D016366:Open Reading Frames; D010802:Phylogeny