Reproductive toxicity of β-diketone antibiotic mixtures to zebrafish (Danio rerio).
So far, few data are available on the reproductive toxicological assessment of β-diketone antibiotics (DKAs), a class of ubiquitous pseudo-persistent pollutant, in zebrafish (Danio rerio). Herein, we reported the reproductive effects of DKAs by means of transcriptome analysis (F1-zebrafish), changes in a series of reproductive indices (F0-zebrafish) and histopathological observations. A total of 1170, 983 and 1399 genes were found to be differentially expressed when compared control vs. 6.25mg/L, control vs. 12.5mg/L and 6.25 vs. 12.5mg/L DKA-exposure treatments, respectively. Among three comparison groups, 670, 569 and 821 genes were respectively assigned for GO analyses based on matches with sequences of known functions. In 149 KEGG-noted metabolic pathways, the preferential one was the MAPK (mitogen-activated protein kinase) signaling pathway, followed by oxidative phosphorylation, neuroactive ligand-receptor interaction and so on. By qPCR verification, 6 genes (c6ast4, igfbp1b, mrpl42, tnnc2, emc4 and ddit4) showed consistent gene expression with those identified by transcriptome sequencing. Due to DKA-exposure, the concentrations of plasma estradiol and testosterone, and the gonado-somatic index were significantly dose-dependently declined. Also, DKA-exposure led to declining in zebrafish reproductive capacity, reflecting in fertilization, hatchability and egg production. Histopathological observations demonstrated that zebrafish ovary and testis suffered serious damage after DKA-exposure. The 4-oxo-TEMP signals increased obviously with increasing DKA-exposed concentrations, implying disruption of balance between generation and clearance of1O2. In summary, DKAs not only produce reproductive toxicological effects on F0-zebrafish, but also result in adverse consequences for growth and development of F1-zebrafish.
Key Lab of Watershed Sciences and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou 325035, China.
Xuedong
X
Wang
College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Yan
Y
Ma
College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Jinfeng
J
Liu
Key Lab of Watershed Sciences and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou 325035, China.
Xiaohan
X
Yin
College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Zhiheng
Z
Zhang
College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Caihong
C
Wang
Key Lab of Watershed Sciences and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou 325035, China.
Yanyan
Y
Li
College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: whuili@163.com.
Huili
H
Wang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D024841:Fluoroquinolones / Q000737:chemistry / Q000633:toxicity*; D020869:Gene Expression Profiling; D008297:Male; D058977:Molecular Sequence Annotation; D012098:Reproduction / Q000187:drug effects* / Q000235:genetics; D013754:Tetracyclines / Q000737:chemistry / Q000633:toxicity*; D059467:Transcriptome / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D013754:Tetracyclines; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins
Biomarker; Diketone antibiotics; Histopathological change; Reproductive toxicity; Transcriptome analysis; Zebrafish