Etiological agents of viral meningitis in children from a dengue-endemic area, Southeast region of Brazil.
Meningitis is a disease with a global distribution that constitutes a worldwide burden, with viruses as the primary etiologic agents. The range of viral meningitis severity depends mainly on age, immune status and etiological agent. The aim of this work was to investigate the suspected cases of viral meningitis using molecular techniques to confirm the viral infection. The diagnosed virus was correlated with clinical findings and cytochemical parameters in cerebrospinal liquid (CSF) of patients. CSF of 70 children with the presumptive diagnosis of viral meningitis was analyzed by real time PCR (qPCR). Viruses were identified by qPCR in 44 CSF samples (62.9%). Among them, 31 were identified as Enterovirus (ENTV) (70.4%), six as Human herpes virus 3 (HHV-3) (13.6%), five as Dengue virus (DENV) (11.7%), one as Human herpes virus 1-2 (2.3%) and one as Human herpes virus 5 (2.3%). Patients in the HHV-positive groups had increased percentage of polymorphonuclear neutrophils (PMN) (mean of 81%) while the groups of patients with DENV and ENTV had a mean of 30.9%. This study contributes to the knowledge of the epidemiological distribution of viral agents in CNS infections in children. In addition, it raises the relevance of DENV as an agent of CNS infection, and reinforces the importance for molecular in the cases of CNV infection.
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil; Faculdade de Medicina de Diamantina, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina 39100-000, Brazil.
Danilo B
DB
de Oliveira
Hospital Infantil João Paulo II, FHEMIG, Belo Horizonte 30130-110, Brazil.
Talitah M
TM
Candiani
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Ana Paula M
APM
Franco-Luiz
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Gabriel M F
GMF
Almeida
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Jônatas S
JS
Abrahão
Center for Biologics Research and Review, Food and Drug Administration, Silver Spring, MD 20993, USA.
Maria
M
Rios
Neurogenômica, Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte 30190-002, Brazil.
Roney S
RS
Coimbra
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil. Electronic address: kroone@icb.ufmg.br.
Erna G
EG
Kroon
D000704:Analysis of Variance; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D003715:Dengue / Q000134:cerebrospinal fluid / Q000453:epidemiology*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D004770:Enterovirus / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005416:Flavivirus / Q000235:genetics; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008587:Meningitis, Viral / Q000134:cerebrospinal fluid / Q000453:epidemiology* / Q000209:etiology*; D018139:Simplexvirus / Q000235:genetics
D004279:DNA, Viral; D007075:Immunoglobulin M
Dengue virus in CNS; Epidemiology; Molecular diagnosis; Viral meningitis