The clinical significance of anti-mitotic spindle apparatus antibody (MSA) and anti-centromere antibody (ACA) detected in patients with small cell lung cancer (SCLC).
OBJECTIVE
The project is aimed to detect anti-mitotic spindle apparatus antibody (MSA) and anti-centromere antibody (ACA) and explore the clinical value for the diagnosis of small cell lung cancer (SCLC), providing clinical evidence for molecular studies of SCLC.


METHODS
93 SCLC patients, 208 patients with other cancers and 50 healthy controls were enrolled in this study. MSA antibodies were detected by enzyme linked immunosorbent assay (ELISA). MSA, ACA and anti nuclear antibodies (ANA) were examined by indirect immuno-fluorescence (IIF). And the results were retrospectively analyzed.


RESULTS
① the positivity for MSA and ACA by IIF assay was respectively 36.56% and 30.11% in SCLC group, higher than in other tumor groups (P<0.01), ② in correlative analysis, the RR (Relative Ratio) value between MSA and SCLC was as high as 12.93, 12.74, and the RR value of ACA and ANA with SCLC was respectively 4.31 and 3.48. ③ the area under ROC (Receiver operating characteristic) curve (AUC) of MSA detection for SCLC was 0.778, with medium diagnostic value.


CONCLUSIONS
MSA and ACA might serve as a new marker for SCLC because of its high detection rate. These two markers may participate in the occurrence and development of SCLC, resulting from the highly strong risk. So, the study have some application value for early detection, clinical diagnosis and potential treatments of SCLC.
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Liming
L
Tan
2010 Session, The Second Clinical Medical College of Nanchang University Nanchang 330006, P. R. China.
Yuhong
Y
Zhang
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Yongqing
Y
Jiang
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Hua
H
Li
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Juanjuan
J
Chen
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Feng
F
Ming
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Waimei
W
Wang
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Jianlin
J
Yu
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Tingting
T
Zeng
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Yongjian
Y
Tian
Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
Yang
Y
Wu
Small cell lung cancer; anti-centromere antibodies; antinuclear antibodies; human mitotic spindle apparatus antibodies