Isolation and characterization of bacterial endophytes of Curcuma longa L.
Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp. (ECL3), Bacillus pumilis (ECL4), Pseudomonas putida (ECL5), and Clavibacter michiganensis (ECL6). All the strains produced IAA and solubilized phosphate and only two strains produced siderophore (ECL3 and ECL5) during plant growth promoting trait analysis. All the endophytic strains utilized glucose, sucrose and yeast extract as a carbon source where as glycine, alanine, cystine and glutamine as nitrogen source. The strains were mostly sensitive to antibiotic chloramphenicol followed by erythromycin while resistant to polymixin B. The endophytic strains effectively inhibit the growth of Escherichia coli, Klebsiella pneumoniae and some of the fungal strain like Fusarium solani and Alterneria alternata. The strain ECL2 and ECL4 tolerated maximum 8 % of NaCl concentration where as strains ECL5 and ECL6 6 % in salinity tolerance.
Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India.
Ajay
A
Kumar
Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India.
Ritu
R
Singh
Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India.
Akhilesh
A
Yadav
Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India.
D D
DD
Giri
Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India.
P K
PK
Singh
Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India. kdpandey2005@gmail.com.
Kapil D
KD
Pandey
16S rRNA analysis; Antimicrobial activity; Curcuma longa; Endophyte; Substrate utilization