Highlighting the effects of coumarin on adult plants of Arabidopsis thaliana (L.) Heynh. by an integrated -omic approach.
In this study, the effects of the allelochemical coumarin through a metabolomic, proteomic and morpho-physiological approach in Arabidopsis adult plants (25days old) were investigated. Metabolomic analysis evidenced an increment of amino acids and a high accumulation of soluble sugars, after 6days of coumarin treatment. This effect was accompanied by a strong decrease on plant fresh and dry weights, as well as on total protein content. On the contrary, coumarin did not affect leaf number but caused a reduction in leaf area. An alteration of water status was confirmed by a reduction of relative water content and an increase in leaf osmotic potential. Moreover, coumarin impaired plant bio-membranes through an increase of lipid peroxidation and H2O2content suggesting that coumarin treatment might induce oxidative stress. Coumarin reduced the effective quantum yield of the photosystem II, the energy dissipation in the form of heat, the maximum PSII efficiency, the coefficient of the photochemical quenching and the estimated electron transport rate, while it significantly stimulated the fluorescence emission and the coefficient of the non photochemical quenching. Finally, the proteomic characterization of coumarin-treated plants revealed a down-regulation of the ROS detoxifying proteins, responsible of oxidative damage and consequently of physiological cascade effects.
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy. Electronic address: fabrizio.araniti@unirc.it.
Fabrizio
F
Araniti
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Monica
M
Scognamiglio
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Angela
A
Chambery
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Rosita
R
Russo
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Assunta
A
Esposito
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Brigida
B
D'Abrosca
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Antonio
A
Fiorentino
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Antonio
A
Lupini
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy.
Francesco
F
Sunseri
Department of AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, I-89122 Reggio Calabria, RC, Italy; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, via Vivaldi 43, I-81100 Caserta, Italy. Electronic address: mrabenavoli@unirc.it.
Maria Rosa
MR
Abenavoli
D017360:Arabidopsis / Q000187:drug effects* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D003374:Coumarins / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D055432:Metabolomics / Q000379:methods; D040901:Proteomics / Q000379:methods; D014867:Water / Q000378:metabolism
D029681:Arabidopsis Proteins; D003374:Coumarins; D014867:Water; C030123:coumarin; D006861:Hydrogen Peroxide
Chlorophyll a fluorescence; Metabolomic; Oxidative stress; Photoinhibition; Plant water-status; Proteomic