Novel method for measurement of heparin anticoagulant activity using SPR.
A novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive antithrombin III binding of analyte heparin in the solution phase and USP heparin immobilized on chip surface. Heparins, obtained from different animal sources, and low molecular weight heparins were analyzed. The results were reproducible and correlated well with the results of chromogenic assays (correlation coefficient r = 0.98 for anti-Xa and r = 0.94 for anti-IIa). This protocol provides many advantages, significantly minimizing time, cost and the complications of chromogenic assay methods.
Department of Chemistry and Chemical Biology, Troy, NY 12180, USA; College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Jing
J
Zhao
Department of Chemistry and Chemical Biology, Troy, NY 12180, USA.
Xinyue
X
Liu
Department of Chemical and Biological Engineering, Troy, NY 12180, USA.
Anju
A
Malhotra
College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Quanhong
Q
Li
Department of Chemical and Biological Engineering, Troy, NY 12180, USA. Electronic address: zhangf2@rpi.edu.
Fuming
F
Zhang
Department of Chemistry and Chemical Biology, Troy, NY 12180, USA; Department of Chemical and Biological Engineering, Troy, NY 12180, USA; Departments of Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address: linhar@rpi.edu.
Robert J
RJ
Linhardt
D000818:Animals; D000925:Anticoagulants / Q000032:analysis*; D000990:Antithrombin III / Q000378:metabolism*; D002417:Cattle; D006493:Heparin / Q000032:analysis*; D007413:Intestinal Mucosa / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D013045:Species Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D013552:Swine
D000925:Anticoagulants; D000990:Antithrombin III; D006493:Heparin
Anticoagulant activity; Antithrombin III; Heparin; SPR