Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization.
The present investigation focused on screening of a new potent strain for laccase production and optimizing the process parameters to achieve the maximum enzymatic decolourization of textile azo dye Congo red. Seven hydrocarbonoclastic bacterial strains were selected as positive in laccase production in solid medium using 2,6 dimethoxyphenol as an enzyme activity indicator. The best enzyme producer Pseudomonas extremorientalis BU118 showed a maximum laccase activity of about 7000 U/L of wheat bran under solid-state conditions. The influence of different concentrations of dye, enzyme, salt and various incubation times on Congo red decolourization was studied using response surface methodology to find the optimum conditions required for maximum decolourization by P. extremorientalis laccase. The enzyme exhibited a remarkable colour removal capability over a wide range of dye and salt concentrations. The above results show the potential use of this bacterial laccase in the biological treatment of the textile effluent.
BVBGR-LR11ES31, ISBST, University of Manouba, Biotechpole Sidi Thabet, 2020, Ariana, Tunisia. mohamed.naifar@gmail.com.
Mohamed
M
Neifar
BVBGR-LR11ES31, ISBST, University of Manouba, Biotechpole Sidi Thabet, 2020, Ariana, Tunisia.
Habib
H
Chouchane
BVBGR-LR11ES31, ISBST, University of Manouba, Biotechpole Sidi Thabet, 2020, Ariana, Tunisia.
Mouna
M
Mahjoubi
MBA-LR03ES03, FST, University of Tunis El Manar, Campus Universitaire, 2092, Tunis, Tunisia.
Atef
A
Jaouani
BVBGR-LR11ES31, ISBST, University of Manouba, Biotechpole Sidi Thabet, 2020, Ariana, Tunisia.
Ameur
A
Cherif
Azo dye decolourization; Central composite design; Pseudomonas extremorientalis laccase; Response surface methodology; Salt tolerance