Gastric microbiota features associated with cancer risk factors and clinical outcomes: A pilot study in gastric cardia cancer patients from Shanxi, China.
Little is known about the link between gastric microbiota and the epidemiology of gastric cancer. In order to determine the epidemiologic and clinical relevance of gastric microbiota, we used 16 S ribosomal RNA gene sequencing analysis to characterize the composition and structure of the gastric microbial community of 80 paired samples (non-malignant and matched tumor tissues) from gastric cardia adenocarcinoma (GCA) patients in Shanxi, China. We also used PICRUSt to predict microbial functional profiles. Compared to patients without family history of upper gastrointestinal (UGI) cancer in the non-malignant gastric tissue microbiota, patients with family history of UGI cancer had higher Helicobacter pylori (Hp) relative abundance (median: 0.83 vs. 0.38, p = 0.01) and lower alpha diversity (median observed species: 51 vs. 85, p = 0.01). Patients with higher (vs. lower) tumor grade had higher Hp relative abundance (0.73 vs. 0.18, p = 0.03), lower alpha diversity (observed species, 66 vs. 89, p = 0.01), altered beta diversity (weighted UniFrac, p = 0.002) and significant alterations in relative abundance of five KEGG functional modules in non-malignant gastric tissue microbiota. Patients without metastases had higher relative abundance of Lactobacillales than patients with metastases (0.05 vs. 0.01, p = 0.04) in non-malignant gastric tissue microbiota. These associations were observed in non-malignant tissues but not in tumor tissues. In conclusion, this study showed a link of gastric microbiota to a major gastric cancer risk factor and clinical features in GCA patients from Shanxi, China. Studies with both healthy controls and gastric cardia and noncardia cancer cases across different populations are needed to further examine the association between gastric cancer and the microbiota.
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Guoqin
0000-0002-3015-9886
G
Yu
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Nan
N
Hu
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Lemin
L
Wang
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Chaoyu
C
Wang
Department of Epidemiology, Shanxi Cancer Hospital, Taiyuan, Shanxi, 030013, People's Republic of China.
Xiao-You
XY
Han
Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD.
Mike
M
Humphry
Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD.
Jacques
J
Ravel
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Christian C
CC
Abnet
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Philip R
PR
Taylor
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.
Alisa M
AM
Goldstein
D000230:Adenocarcinoma / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D002299:Cardia / Q000382:microbiology / Q000473:pathology; D002681:China; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics / Q000382:microbiology* / Q000473:pathology
D012336:RNA, Ribosomal, 16S
16S rRNA gene; H. pylori; KEGG modules; gastric cancer; microbiome