Characterization of high yield exopolysaccharide produced by Phyllobacterium sp. 921F exhibiting moisture preserving properties.
A new strain bacteria was isolated and named as Phyllobacterium sp. 921F, due to its high production capacity of exopolysaccharide (EPS). Characterization of physico-chemical properties of the EPS and optimization for high production were conducted to aim at industrial applications. The optimum pH and temperature were 7.0 and 30°C, respectively. The following scale-up fermentation was carried out in 30L bioreactor and amounts of EPS (21.9g/L) were produced. The EPS with a molecular mass of 1082kDa was composed of glucose, galactose, and pyruvate. The EPS solution behaved as Newtonian at low concentrations (≤0.3%) and as shear thinning at higher concentration (e.g, 1%). The moisture retention ability of the EPS was found to be superior to hyaluronic acid. Results suggest that Phyllobacterium sp. 921F is a good candidate for large-scale production of the EPS which might be utilized in food and cosmetics industries.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PR China.
Yinping
Y
Li
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
Gaoli
G
Zhang
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
Chunying
C
Du
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
Haijin
H
Mou
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
Jiefen
J
Cui
School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PR China.
Huashi
H
Guan
Biology Department, Jackson State University, Jackson, MS 39217, USA.
Hueymin
H
Hwang
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China; State Key Laboratory of Bioactive Seaweed Substances, Qingdao 266400, PR China. Electronic address: pengwang@ouc.edu.cn.
Peng
P
Wang
D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009584:Nitrogen / Q000494:pharmacology; D059127:Phyllobacteriaceae / Q000187:drug effects / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry*; D012212:Rheology; D013696:Temperature; D014783:Viscosity
D011135:Polysaccharides, Bacterial; D002244:Carbon; D009584:Nitrogen
Exopolysaccharide; Fermentation; Moisture retention and absorption ability; Phyllobacterium sp. 921F; Rheological properties