Magnetic nanobubbles with potential for targeted drug delivery and trimodal imaging in breast cancer: an in vitro study.
OBJECTIVE
The aim of this study was to improve tumor-targeted therapy for breast cancer by designing magnetic nanobubbles with the potential for targeted drug delivery and multimodal imaging.


METHODS
Herceptin-decorated and ultrasmall superparamagnetic iron oxide (USPIO)/paclitaxel (PTX)-embedded nanobubbles (PTX-USPIO-HER-NBs) were manufactured by combining a modified double-emulsion evaporation process with carbodiimide technique. PTX-USPIO-HER-NBs were examined for characterization, specific cell-targeting ability and multimodal imaging.


RESULTS
PTX-USPIO-HER-NBs exhibited excellent entrapment efficiency of Herceptin/PTX/USPIO and showed greater cytotoxic effects than other delivery platforms. Low-frequency ultrasound triggered accelerated PTX release. Moreover, the magnetic nanobubbles were able to enhance ultrasound, magnetic resonance and photoacoustics trimodal imaging.


CONCLUSIONS
These results suggest that PTX-USPIO-HER-NBs have potential as a multimodal contrast agent and as a system for ultrasound-triggered drug release in breast cancer.
Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Weixiang
W
Song
Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Yindeng
Y
Luo
Department of Ultrasound, the First Affiliated Hospital of Chongqing Medical University, No. 1, Youyi Rd, Yuzhong District, 400016 Chongqing, China.
Yajing
Y
Zhao
Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Xinjie
X
Liu
Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Jiannong
J
Zhao
Department of Ultrasound, the First Affiliated Hospital of Chongqing Medical University, No. 1, Youyi Rd, Yuzhong District, 400016 Chongqing, China.
Jie
J
Luo
Department of Ultrasound, Institute of Ultrasound Imaging, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Qunxia
Q
Zhang
Department of Ultrasound, Institute of Ultrasound Imaging, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Haitao
H
Ran
Department of Ultrasound, Institute of Ultrasound Imaging, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Zhigang
Z
Wang
Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Rd, Yuzhong District, 400010 Chongqing, China.
Dajing
D
Guo
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001940:Breast / Q000000981:diagnostic imaging / Q000187:drug effects; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D045423:Microbubbles; D064847:Multimodal Imaging / Q000379:methods; D017239:Paclitaxel / Q000008:administration & dosage* / Q000494:pharmacology; D061088:Photoacoustic Techniques / Q000379:methods; D000068878:Trastuzumab / Q000008:administration & dosage* / Q000494:pharmacology; D014463:Ultrasonography / Q000379:methods
D000970:Antineoplastic Agents; D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; D000068878:Trastuzumab; D017239:Paclitaxel
PEGlyated PLGA; magnetic resonance; nanobubble; photoacoustic; target; ultrasound