Dentin bond optimization using the dimethyl sulfoxide-wet bonding strategy: A 2-year in vitro study.
This study evaluated a new approach, named dimethyl sulfoxide (DMSO)-wet bonding, to produce more desirable long-term prospects for the ultrafine interactions between synthetic polymeric biomaterials and the inherently hydrated dentin substrate.



Sound third molars were randomly restored with/without DMSO pretreatment using a total-etch (Scocthbond Multipurpose: SBMP) and a self-etch (Clearfil SE Bond: CF) adhesive systems. Restored teeth (n=10)/group were sectioned into sticks and submitted to different analyses: micro-Raman determined the degree of conversion inside the hybrid layer (DC); resin-dentin microtensile bond strength and fracture pattern analysis at 24h, 1year and 2 years of aging; and nanoleakage evaluation at 24h and 2 years.



DMSO-wet bonding produced significantly higher 24h bond strengths for SBMP that were sustained over the two-year period, with significantly less adhesive failures. Similarly, DMSO-treated CF samples presented significantly higher bond strength than untreated samples at two years. Both adhesives had significant less adhesive failures at 2 years with DMSO. DMSO had no effect on DC of SBMP, but significantly increased the DC of CF. DMSO-treated SBMP samples presented reduced silver uptake compared to untreated samples after aging.



Biomodification of the dentin substrate by the proposed strategy using DMSO is a suitable approach to produce more durable hybrid layers with superior ability to withstand hydrolytic degradation over time. Although the active role of DMSO on dentin bond improvement may vary according to monomer composition, its use seems to be effective on both self-etch and etch-and-rinse bonding mechanisms.
Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, Finland; Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Av. Limeira Piracicaba, SP 13414-903, Brazil. Electronic address: thiagohs@hotmail.com.
Thiago Henrique Scarabello
TH
Stape
Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland and Helsinki University Hospital, Helsinki, Finland; Research Unit of Oral Health Sciences, and Medical Research Center Oulu (MRC Oulu), Oulu University Hospital and University of Oulu, Oulu, Finland. Electronic address: leo.tjaderhane@helsinki.fi.
Leo
L
Tjäderhane
Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, Finland; Department of Restorative Dentistry and Cariology, University of Turku, Turku, Finland. Electronic address: arztez@utu.fi.
Arzu
A
Tezvergil-Mutluay
Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Av. Limeira Piracicaba, SP 13414-903, Brazil. Electronic address: cristiane@fyodontologia.com.br.
Cristiane Rumi Fujiwara
CR
Yanikian
School of Dentistry, Department of Restorative Dentistry, University Estadual de Ponta Grossa, Campus Uvaranas - Bloco M - 52B 2 Piso, Avenida General Carlos Cavalcanti, 4748 Ponta Grossa, PR, Brazil.
Anna Luiza
AL
Szesz
School of Dentistry, Department of Restorative Dentistry, University Estadual de Ponta Grossa, Campus Uvaranas - Bloco M - 52B 2 Piso, Avenida General Carlos Cavalcanti, 4748 Ponta Grossa, PR, Brazil. Electronic address: aloguercio@hotmail.com.
Alessandro Dourado
AD
Loguercio
Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Av. Limeira Piracicaba, SP 13414-903, Brazil. Electronic address: martins@fop.unicamp.br.
Luís Roberto Marcondes
LR
Martins
D000134:Acid Etching, Dental; D001840:Dental Bonding; D003804:Dentin; D017220:Dentin-Bonding Agents; D004121:Dimethyl Sulfoxide; D008422:Materials Testing; D019279:Resin Cements; D013718:Tensile Strength
D017220:Dentin-Bonding Agents; D019279:Resin Cements; D004121:Dimethyl Sulfoxide
Bond durability; Bond strength; DMSO; Degree of conversion; Dentin bonding; Hybrid layer; MicroRaman; Nanoleakage; Wet-bonding