Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila.
Mitochondrial quality control is a key process in tissues with high energy demands, such as the brain and muscles. Recent studies using Drosophila have revealed that the genes responsible for familial forms of juvenile Parkinson's disease (PD), PINK1 and Parkin regulate mitochondrial function and motility. Cell biological analysis using mammalian cultured cells suggests that the dysregulation of mitophagy by PINK1 and Parkin leads to neurodegeneration in PD. In this chapter, we describe the methods to monitor mitochondrial morphology in the indirect flight muscles of adult Drosophila and Drosophila primary cultured neurons and the methods to analyze the motility of mitochondria in the axonal transport of living larval motor neurons.
Department of Treatment and Research in Multiple Sclerosis and Neuro-Intractable Disease, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan.
Tsuyoshi
T
Inoshita
Department of Treatment and Research in Multiple Sclerosis and Neuro-Intractable Disease, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan.
Kahori
K
Shiba-Fukushima
Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan.
Hongrui
H
Meng
Department of Neurology, Juntendo University School of Medicine, Hongo, Bunkyo, Tokyo, Japan.
Nobutaka
N
Hattori
Department of Research for Parkinson's Disease, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan. yzimai@juntendo.ac.jp.|Department of Neurology, Juntendo University Graduate School of Medicine, Tokyo, 113-8421, Japan. yzimai@juntendo.ac.jp.
Yuzuru
Y
Imai
D000818:Animals; D001370:Axonal Transport; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism; D057054:Molecular Imaging; D009474:Neurons / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D000099202:PTEN-Induced Putative Kinase
D029721:Drosophila Proteins; D017346:Protein Serine-Threonine Kinases; D044767:Ubiquitin-Protein Ligases; C534484:PINK1 protein, Drosophila; D000099202:PTEN-Induced Putative Kinase; C487430:park protein, Drosophila
Axonal transport; Fluorescence imaging; Mitochondrial fusion and fission; Muscle mitochondria; Primary neuron culture