Genetic diversity of symbiotic Paraburkholderia species isolated from nodules of Mimosa pudica (L.) and Phaseolus vulgaris (L.) grown in soils of the Brazilian Atlantic Forest (Mata Atlântica).
Some species of the genus Paraburkholderia that are able to nodulate and fix nitrogen in symbiosis with legumes are called β-rhizobia and represent a group of ecological and biotechnological importance. We used Mimosa pudica and Phaseolus vulgaris to trap 427 rhizobial isolates from rhizospheric soil of Mimoseae trees in the Brazilian Atlantic Forest. Eighty-four representative strains were selected according to the 16S rRNA haplotypes and taxonomically characterized using a concatenated 16S rRNA-recA phylogeny. Most strains were assembled in the genus Paraburkholderia, including Paraburkholderia sabiae and Pa. nodosa. Mesorhizobium (α-rhizobia) and Cupriavidus (β-rhizobia) were also isolated, but in smaller proportions. Multilocus sequence analysis and BOX-PCR analyses indicated that six clusters of Paraburkholderia represent potential new species. In the phylogenetic analysis of the nodC gene, the majority of the strains were positioned in the same groups as in the 16S rRNA-recA tree, indicative of stability and vertical inheritance, but we also identified horizontal transfer of nodC in Pa. sabiae. All α- and β-rhizobial species were trapped by both legumes, although preferences of the host plants for specific rhizobial species have been observed.
Soil Biotechnology Laboratory, Embrapa Soja, C.P. 231, 86001-970, Londrina, PR, Brazil.|Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, C.P. 10.011, 86057-970, Londrina, PR, Brazil.|IRD, Cirad, Univ. Montpellier, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), Campus de Baillarguet 34398 Montpellier, France.
Rebeca Fuzinatto
RF
Dall'Agnol
IRD, Cirad, Univ. Montpellier, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), Campus de Baillarguet 34398 Montpellier, France.|Embrapa Recursos Genéticos e Biotecnologia, LIMPP Laboratory, C.P. 02372, 70770-917, Brasília, DF, Brazil.
Caroline
C
Bournaud
Embrapa Agrobiologia, C.P 74.505, 23891-000, Seropédica, RJ, Brazil.
Sérgio Miana
SM
de Faria
IRD, Cirad, Univ. Montpellier, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), Campus de Baillarguet 34398 Montpellier, France.|IRD, Cirad, Univ. Montpellier, Interactions Plantes Microorganismes Environnement (IPME), 34394 Montpellier, France.
Gilles
G
Béna
IRD, Cirad, Univ. Montpellier, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), Campus de Baillarguet 34398 Montpellier, France.|IRD, Cirad, Univ. Montpellier, Interactions Plantes Microorganismes Environnement (IPME), 34394 Montpellier, France.
Lionel
L
Moulin
Soil Biotechnology Laboratory, Embrapa Soja, C.P. 231, 86001-970, Londrina, PR, Brazil.
Mariangela
M
Hungria
D020562:Betaproteobacteria / Q000235:genetics*; D001938:Brazil; D049921:Cupriavidus / Q000145:classification; D065928:Forests; D014644:Genetic Variation; D031298:Mimosa / Q000382:microbiology*; D027805:Phaseolus / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D012987:Soil; D012988:Soil Microbiology; D013559:Symbiosis
D012336:RNA, Ribosomal, 16S; D012987:Soil
beta-rhizobia; biological nitrogen fixation; diazotrophic bacteria; nodulation; phylogeny