The effects of antiviral treatment on breast cancer cell line.
BACKGROUND
Recent studies have revealed the positive antiproliferative and cytotoxic effects of antiviral agents in cancer treatment. The real effect of adjuvant antiviral therapy is still controversial due to the lack of studies in biochemical mechanisms. Here, we studied the effect of the antiviral agent acyclovir on morphometric and migratory features of the MCF7 breast cancer cell line. Molecular levels of various proteins have also been examined.


METHODS
To evaluate and assess the effect of antiviral treatment on morphometric, migratory and other cellular characteristics of MCF7 breast cancer cells, the following experiments were performed: (i) MTT assay to measure the viability of MCF7 cells; (ii) Colony formation ability by soft agar assay; (iii) Morphometric characterization by immunofluorescent analysis using confocal microscopy; (iv) wound healing and transwell membrane assays to evaluate migration and invasion capacity of the cells; (v) ELISA colorimetric assays to assess expression levels of caspase-3, E-cadherin and enzymatic activity of aldehyde dehydrogenase (ALDH).


RESULTS
We demonstrate the suppressive effect of acyclovir on breast cancer cells. Acyclovir treatment decreases the growth and the proliferation rate of cells and correlates with the upregulated levels of apoptosis associated cytokine Caspase-3. Moreover, acyclovir inhibits colony formation ability and cell invasion capacity of the cancer cells while enhancing the expression of E-cadherin protein in MCF7 cells. Breast cancer cells are characterized by high ALDH activity and associated with upregulated proliferation and invasion. According to this study, acyclovir downregulates ALDH activity in MCF7 cells.


CONCLUSIONS
These results are encouraging and demonstrate the possibility of partial suppression of cancer cell proliferation using an antiviral agent. Acyclovir antiviral agents have a great potential as an adjuvant therapy in the cancer treatment. However, more research is necessary to identify relevant biochemical mechanisms by which acyclovir induces a potent anti-cancer effect.
National Laboratory Astana, Nazarbayev University, Qabanbay Batyr Avenue 53, Astana, 010000 Kazakhstan.
Madina
M
Shaimerdenova
Nazarbayev University Research and Innovation System, Nazarbayev University, Astana, Kazakhstan.
Orynbassar
O
Karapina
National Laboratory Astana, Nazarbayev University, Qabanbay Batyr Avenue 53, Astana, 010000 Kazakhstan.
Damel
D
Mektepbayeva
Locus Solutions LLC, Solon, OH USA.
Kenneth
K
Alibek
National Laboratory Astana, Nazarbayev University, Qabanbay Batyr Avenue 53, Astana, 010000 Kazakhstan.
Dana
D
Akilbekova
ALDH activity; Acyclovir; Antiproliferative effect; MCF7 breast cancer cell line