Physiological role of exogenous nitric oxide in improving performance, yield and some biochemical aspects of sunflower plant under zinc stress.
The present study was undertaken to examine the possible roles of sodium nitroprusside in protection against oxidative damage due to zinc toxicity in sunflower plants. Physiochemical parameters in sunflower plants exposed to Zn2+(100, 200 and 300 mg/kg soil) alone or combined with SNP were measured. The results showed that excess of Zn decreased plant growth, seed yield components and photosynthetic pigments content. On the other hand, Zn stress increased the level of non-enzymatic antioxidants (ascorbic acid and reduced glutathione) and enzymatic antioxidants (superoxide dismutase, ascrobate peroxidase and glutathione reductase), coupled with the appearance of novel protein bands. Furthermore, Zn stress increased Zn content in roots and shoots. The amounts of Zn in roots were higher than shoots. A marked increase in total saturated fatty acids accompanied by a decrease in total unsaturated fatty acids was observed. Exogenously application of SNP (20 μM) increased growth parameters, photosynthetic pigments content, ascorbic acid and glutathione contents, antioxidant enzyme activities and the quality of the oil in favour of the increase of unsaturated fatty acids. Moreover, SNP application increased Zn concentration in roots and inhibited Zn accumulation in shoots. Therefore, it is concluded that SNP treatment can help reduce Zn toxicity in sunflower plants.
Biological and Geological Sciences Department, Faculty of Education, Ain Shams University El Makres St. Roxy, Cairo , Egypt Post code: 1575.
Samia Ageeb
SA
Akladious
Biological and Geological Sciences Department, Faculty of Education, Ain Shams University El Makres St. Roxy, Cairo , Egypt Post code: 1575.
Heba Ibrahim
HI
Mohamed
D060387:Ascorbate Peroxidases / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006368:Helianthus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000378:metabolism / Q000494:pharmacology; D009599:Nitroprusside / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000633:toxicity*
D005227:Fatty Acids; D020030:Nitric Oxide Donors; D010940:Plant Proteins; D002734:Chlorophyll; D009599:Nitroprusside; D009569:Nitric Oxide; D002338:Carotenoids; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D015032:Zinc; D001205:Ascorbic Acid
Helianthus annuus; enzymatic antioxidants; fatty acids; non-enzymatic antioxidants; protein electrophoresis