Factors affecting the quality of cryoprecipitate.
BACKGROUND
Many variables affect the quality of cryoprecipitate (CRYO). We investigated the effect of freezing techniques and ABO blood groups on the quality of CRYO with respect to factor VIII: C and fibrinogen levels.


METHODS
Ninety-six whole blood units each collected from in-house (Group I) and blood donation camps outside the hospital premises (Group II) were processed for CRYO preparation. Within each group, half the number of plasma units was frozen using blast freezer and another half using the conventional freezer. The CRYOs from blood groups A, B, and O were equally distributed, i.e. 32 within each of the Groups I and II. The fibrinogen and factor VIII: C levels in CRYO were analyzed using single-stage clotting assay.


RESULTS
In Group I, the mean ± standard deviation percentage recovery of factor VIII levels in CRYO prepared using the conventional freezer and blast freezer were 58.5% ±16.2% and 66.7% ±16.4%, respectively, and in Group II, it was 55.3% ±17.6% and 70.4% ±13.4%, respectively. Recovery of factor VIII was higher in CRYO prepared using blast freezer than that of CRYO prepared using conventional freezer (P< 0.000). In Group II, CRYOs prepared using blast freezer had higher percent recovery of fibrinogen than that of Group I. In both the groups, the mean factor VIII levels in blood group A were higher than that of factor VIII levels in the blood group O CRYO.


CONCLUSIONS
The factor VIII recovery in CRYO improves significantly with higher baseline factor VIII: C levels, blood group A donor, and rapid freezing using blast freezer. Rapid freezing also increases the fibrinogen yield.
Department of Transfusion Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Rajeswari
R
Subramaniyan
Department of Transfusion Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Neelam
N
Marwaha
Department of Transfusion Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Ashish
A
Jain
Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Jasmina
J
Ahluwalia
ABO blood groups; blast freezer; cryoprecipitate; factor VIII: C; fibrinogen