Isolation and in silico characterization of novel esterase gene with β-lactamase fold isolated from metagenome of north western Himalayas.
An esterase-producing clone Aph2 was isolated from the Apharwat soil metagenomic library, a mountain peak in NW Himalayas. ORF 2 (Est Ac) of clone Aph2 corresponds to 271 aa protein and showed 26 % sequence similarity to carboxylesterase gene of Synechococcus sp. JA-2-3B. Est Ac contains nucleophilic Ser in S68-X-X-K71motif of β-lactamases with Tyr Y103. The conserved sequences are common with family VIII carboxylesterase and class C β-lactamase sequences. Phylogenetic analysis revealed that Est Ac sequence is closely related to esterase than to β-lactamases. In silico 3D protein structure of Est Ac was generated using MODELLER software (9.10 version). Model was generated on the basis of carboxylesterase template (PDB:1CI8) of Est B (Burkholderia gladioli) and the stereochemical parameters of the model generated were satisfactory. Docking with diisopropyl-fluorophosphate confirmed catalytic activity of Ser68present in S-X-X-K motif.
School of Biotechnology, University of Jammu, Jammu, 180006, India.
Avneet Kour
AK
Sudan
School of Biotechnology, University of Jammu, Jammu, 180006, India. jyotimetagenomic@gmail.com.
Jyoti
J
Vakhlu
DFP; Docking; Esterases; Homology modelling; Multiple displacement amplification; β-Lactamases