Bovine serum albumin as an effective sensitivity enhancer for peptide-based amperometric biosensor for ultrasensitive detection of prostate specific antigen.
Bovine serum albumin (BSA) was firstly implemented as an effective sensitivity enhancer for a peptide-based amperometric biosensor for the ultrasensitive detection of prostate specific antigen (PSA). A porous and conductive substrate of chitosan-lead ferrocyanide-(poly(diallyldimethylammonium chloride)-graphene oxide) was in-situ generated on a glassy carbon electrode (GCE), in which Pb2[Fe(CN)6] served as a novel redox species with strong current signal at -0.46 V (vs. Ag/AgCl). Poly(diallyldimethylammonium chloride)-graphene oxide was applied to improve conductivity of the substrate. After adsorbing Pb2+for signal amplification, chitosan provided active sites to simultaneously immobilize peptides and 1-aminopropyl-3-methylimidazolium chloride by glutaraldehyde. To enhance the sensitivity, BSA was chemically linked to the immobilized peptide, behaving as a serious decrease of current signal for BSA hindering the electron transfer. The dramatic increase of current signal of the biosensor was obtained by PSA cleaving the immobilized BSA-peptide. The proposed biosensor exhibited a detection limit of 1fgmL-1for PSA and its sensitivity was seven-fold higher than previous works.
Department of Chemistry, Capital Normal University, Beijing 100048, China.
Zhongxue
Z
Tang
Department of Chemistry, Capital Normal University, Beijing 100048, China.
Yuanyuan
Y
Fu
Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: mazhanfang@cnu.edu.cn.
Zhanfang
Z
Ma
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D048271:Chitosan / Q000737:chemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008297:Male; D010087:Oxides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D010087:Oxides; D010455:Peptides; D012710:Serum Albumin, Bovine; D006108:Graphite; D048271:Chitosan; D017430:Prostate-Specific Antigen
Bovine serum albumin; Cancer marker; Chitosan; PDDA-GO; Pb(2)[Fe(CN)(6)]; Peptides