Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa.
The role of the alkaline protease (AprA) in pyocyanin production in Pseudomonas aeruginosa was investigated. AprA was overproduced when a plasmid carrying the aprA gene was introduced to an aprA-deletion mutant strain, EG03; thus, aprA-complemented EG03 was used as an overproducing strain. The complemented strain produced higher pyocyanin than the mutant strain in all commercially available media evaluated. Particularly, pyocyanin production was higher in the complemented than in the parental strain in brain-heart infusion and tryptic soy broths. These results suggested that protein degradation products by AprA were utilized for pyocyanin production. Protein-rich media were used in subsequent validation studies. Similar results were obtained when the basal medium was supplemented with casein or skim milk as the sole organic nitrogen source. However, gelatin failed to induce abundant pyocyanin production in the complemented strain, despite the presence of protein degradation products by AprA as assessed by SDS-PAGE. Thus, gelatin degradation products may not be suitable for pyocyanin synthesis. In conclusion, AprA could contribute to pyocyanin production in the presence of several proteins or peptides.
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Kazuhiro
K
Iiyama
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.|Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Eigo
E
Takahashi
Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Jae Man
JM
Lee
Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Hiroaki
H
Mon
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.|Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Mai
M
Morishita
Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Takahiro
T
Kusakabe
Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Chisa
C
Yasunaga-Aoki
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002364:Caseins / Q000378:metabolism / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005780:Gelatin / Q000378:metabolism; D005816:Genetic Complementation Test; D008892:Milk / Q000378:metabolism; D059748:Proteolysis; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D011710:Pyocyanine / Q000096:biosynthesis*; D037521:Virulence Factors / Q000235:genetics
D001426:Bacterial Proteins; D002364:Caseins; D003470:Culture Media; D037521:Virulence Factors; D005780:Gelatin; D011710:Pyocyanine; D010450:Endopeptidases; C087822:alkaline protease
Pseudomonas aeruginosa; alkaline protease; pathogenicity; protein degradation; pyocyanin; virulence factor