TGF-β1 promotes human gastric carcinoma SGC7901 cells invasion by inducing autophagy.
To investigate the role of TGF-b1 in autophagy and invasion ability in human gastric carcinoma cell line SGC7901.



Cultured SGC7901 cells were treated with different concentrations of TGF-b1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-b1 on invasion ability of SGC7901 cells was detected with transwell method.



The results demonstrated that TGF-b1 was able to induced autophagy of SGC7901 cells in a dose-dependent manner. Autophagy inhibitor 3-MA could inhibit TGF-b1 upregulated autophagy. Furthermore, TGF-b1 significantly enhances the invasion ability of SGC7901 cells. However, autophagy inhibitor 3-MA could effectively reverse this process.



TGF-b1 enhances SGC7901 cells migration by inducing autophagy.
Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical Univerisity, Beijing, China. limingzhe0213@163.com.
J
J
Shen
D-S
DS
Zhao
M-Z
MZ
Li
D001343:Autophagy / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D053773:Transforming Growth Factor beta1