The contribution of dietary restriction to extended longevity in the malaria vector Anopheles coluzzii.
BACKGROUND
Variation in longevity has long been of interest in vector biology because of its implication in disease transmission through vectorial capacity. Recent studies suggest that Anopheles coluzzii adults persist during the ~7 month dry season via aestivation. Recently there has been a growing body of evidence linking dietary restriction and low ratio of dietary protein to carbohydrate with extended longevity of animals. Here, we evaluated the effects of dietary restriction and the protein : carbohydrate ratio on longevity of An. coluzzii.


RESULTS
In our experiment, we combined dietary regimes with temperature and relative humidity to assess their effects on An. coluzzii longevity, in an attempt to simulate aestivation under laboratory conditions. Our results showed significant effects of both the physical and the dietary variables on longevity, but that diet regimen had a considerably greater effect than those of the physical conditions. Higher temperature and lower humidity reduced longevity. At 22 °C dietary protein (blood) shortened longevity when sugar was not restricted (RH = 85%), but extended longevity when sugar was restricted (RH = 50%).


CONCLUSIONS
Dietary restriction extended longevity in accord with predictions, but protein : carbohydrate ratio had a negligible effect. We identified conditions that significantly extend longevity in malaria vectors, however, the extent of increase in longevity was insufficient to simulate aestivation.
Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD, 20852, USA. roy.faiman@nih.gov.
Roy
http://orcid.org/0000-0002-9997-7801
R
Faiman
Charles Perkins Centre, University of Sydney, Sydney, 2006, Australia.
Samantha
S
Solon-Biet
Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD, 20852, USA.
Margery
M
Sullivan
Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD, 20852, USA.|Office of Global Health Diplomacy, U.S. Department of State, 1800 G Street NW, Suite 10300, Washington, DC, 20006, USA.
Diana L
DL
Huestis
Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD, 20852, USA.
Tovi
T
Lehmann
D000821:Animal Feed; D000818:Animals; D000852:Anopheles / Q000502:physiology*; D002241:Carbohydrates / Q000008:administration & dosage; D004032:Diet / Q000379:methods; D005248:Feeding Methods; D008136:Longevity; D000072138:Mosquito Vectors; D011506:Proteins / Q000008:administration & dosage
D002241:Carbohydrates; D011506:Proteins
Aestivation; Anopheles coluzzii; Dietary restriction; Dry season; Longevity