Maintaining the Phenotype Stability of Chondrocytes Derived from MSCs by C-Type Natriuretic Peptide.
Mesenchymal stem cells (MSCs) play a critical role in cartilage tissue engineering. However, MSCs-derived chondrocytes or cartilage tissues are not stable and easily lose the cellular and cartilage phenotype during long-term culturein vitroor implantationin vivo. As a result, chondrocytes phenotypic instability can contribute to accelerated ossification. Thus, it is a big challenge to maintain their correct phenotype for engineering hyaline cartilage. As one member of the natriuretic peptide family, C-type natriuretic peptide (CNP) is found to correlate with the development of the cartilage, affect the chondrocytes proliferation and differentiation. Besides, based on its biological effects on protection of extracellular matrix of cartilage and inhibition of mineralization, we hypothesize that CNP may contribute to the stability of chondrocyte phenotype of MSCs-derived chondrocytes.
Department of Stomatology, Chinese People's Liberation Army General HospitalBeijing, China; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical SciencesBeijing, China.
Quan
Q
Shi
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical SciencesBeijing, China; School of Biological Science and Medical Engineering, Beihang UniversityBeijing, China.
Zhiyong
Z
Qian
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences Beijing, China.
Donghua
D
Liu
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical SciencesBeijing, China; Stomatology Center, General Hospital of Armed Police ForcesBeijing, China.
Jie
J
Sun
Department of Stomatology, Chinese People's Liberation Army General Hospital Beijing, China.
Juan
J
Xu
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences Beijing, China.
Ximin
X
Guo
C-type natriuretic peptide; chondrogenic differentiation; hypothesis; mesenchymal stem cells; phenotype stability