Mutations in the plastidic ACCase gene endowing resistance to ACCase-inhibiting herbicide in Phalaris minor populations from India.
Littleseed canarygrass (Phalaris minor Retz.) is one of the most common and troublesome weeds infesting wheat crop in India. Repeated use during the last two decades of the ACCase-inhibiting herbicide (clodinafop) to control this weed has resulted in the occurrence of resistance. Fifty-three P. minor populations were collected from wheat fields in Haryana and Punjab states of India. The dose-response assays indicated that 29 populations were resistant, 23 populations were susceptible and one population was moderately resistant to clodinafop. Sequence analysis of the CT domain of ACCase gene among resistant and susceptible populations revealed two non-synonymous mutations, Trp2027to Cys and Ile2041to Asn in the resistant populations. Allele-specific PCR markers were developed to differentiate between wild-type and resistant codons at positions 2027 and 2041 of ACCase in P. minor which enables molecular assays for rapid detection and resistance diagnosis for efficient weed management in wheat. This is the first report from India of a target site mutation corresponding to resistance to clodinafop in P. minor.
ICAR-Indian Institute of Wheat and Barley Research, Post Box 158, Karnal, 132001, India.|Maharishi Markandeshwar University, Mullana, India.
Nishu
N
Raghav
ICAR-Indian Institute of Wheat and Barley Research, Post Box 158, Karnal, 132001, India. rajenderkhokhar@yahoo.com.
Rajender
R
Singh
ICAR-Indian Institute of Wheat and Barley Research, Post Box 158, Karnal, 132001, India.
Rajender Singh
RS
Chhokar
ICAR-Indian Institute of Wheat and Barley Research, Post Box 158, Karnal, 132001, India.
Davinder
D
Sharma
Maharishi Markandeshwar University, Mullana, India.
Raman
R
Kumar
Acetyl-coenzyme A carboxylase; Herbicide resistance; Mutation; Phalaris minor; Single nucleotide polymorphism