Development of matrix solid-phase dispersion coupled with high-performance liquid chromatography for determination of jolkinolide A and jolkinolide B in Euphorbia fischeriana Steud.
A novel and simple method was established for the extraction and determination of jolkinolide A and B in Euphorbia fischeriana Steud. using matrix solid-phase dispersion (MSPD) extraction and high-performance liquid chromatography (HPLC). The optimised conditions for the MSPD extraction were determined to be that silica gel was served as dispersant, the mass ratio of sample to silica gel was selected to be 1:4, and 5 mL of acetonitrile was used as elution solvent. The method exhibited a good performance in terms of linearity (r2≥ 0.9997) and the limits of detection in the range of 0.052-0.065 μg mL-1. The recoveries were in the range of 90.2-98.9% with relative standard deviations (RSDs) ranging from 1.3 to 3.5%. The extraction efficiencies obtained by the MSPD were higher than other extraction method with less cost of sample and solvent. At last, the optimised method was applied for analysing real samples.
a Institute of Medicine and Drug Research , Qiqihar Medical University , Qiqihar , China.
Defu
D
Cai
b The First Affiliated Hospital , Qiqihar Medical University , Qiqihar , China.
Weiyu
W
Zhai
a Institute of Medicine and Drug Research , Qiqihar Medical University , Qiqihar , China.
Qi
Q
Zhang
a Institute of Medicine and Drug Research , Qiqihar Medical University , Qiqihar , China.
Jianhua
J
Liu
a Institute of Medicine and Drug Research , Qiqihar Medical University , Qiqihar , China.
Yu
Y
Sun
a Institute of Medicine and Drug Research , Qiqihar Medical University , Qiqihar , China.
Lei
L
Liu
a Institute of Medicine and Drug Research , Qiqihar Medical University , Qiqihar , China.
Jicheng
J
Liu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis*; D028481:Euphorbia / Q000737:chemistry*; D057230:Limit of Detection; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents
D004224:Diterpenes; D012997:Solvents; C057915:jolkinolide A; C057914:jolkinolide B
Euphorbia fischeriana Steud; Matrix solid-phase dispersion; jolkinolide A; jolkinolide B; liquid chromatography