Spontaneous and specific binding of enterohemorrhagic Escherichia coli to overoxidized polypyrrole-coated microspheres.
Specific identification of enterohemorrhagic Escherichia coli was achieved using microspheres coated with overoxidized polypyrrole. The microspheres are well dispersed in aqueous media, and they specifically, spontaneously, and efficiently bind E. coli O157:H7 through surface area effects. In addition, we found that light-scattering by a single microsphere depended linearly on the number of bound cells.
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp.
Xueling
X
Shan
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp.
Takuya
T
Yamauchi
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp and GreenChem. Inc., 19-19 Tsuruta, Nishi, Sakai, Osaka 593-8323, Japan.
Yojiro
Y
Yamamoto
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp and Department of Chemistry, Prince of Songkla University, Hat Yai, Songkla 90112, Thailand.
Saroh
S
Niyomdecha
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp.
Kengo
K
Ishiki
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp.
Dung Q
DQ
Le
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp.
Hiroshi
http://orcid.org/0000-0002-1664-7816
H
Shiigi
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan. shii@chem.osakafu-u.ac.jp.
Tsutomu
T
Nagaoka
D001665:Binding Sites; D054324:Enterohemorrhagic Escherichia coli / Q000737:chemistry* / Q000166:cytology; D008863:Microspheres; D010084:Oxidation-Reduction; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole