Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture.
The blue mussel (Mytilus edulis) is known as a robust bivalve species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated 17 strains from induced mortality events in healthy wild-caught blue mussel adults and demonstrated that they caused between 17% and 98% mortality in blue mussel larvae in a newly developed, highly controlled immersion challenge test model. Eight of the isolates belong to the Splendidus clade of vibrios, while the other isolates belong to the genus Photobacterium. The genomes of the most virulent Vibrio isolate and the most virulent Photobacterium isolate were sequenced and contained several genes encoding factors that have previously been linked to virulence towards bivalves. In vitro tests confirmed that all 17 isolates were positive for these virulence factors. The sequenced genomes also contained a remarkably high number of multidrug resistance genes. We therefore assessed the sensitivity of all isolates to a broad range of antibiotics and found that there were indeed many strong positive correlations between the sensitivities of the isolates to different antibiotics. Our data provide an ecological insight into mass mortality in blue mussels as they indicate that wild mussels contain a reservoir of pathogenic bacteria.
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Mieke
M
Eggermont
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Peter
P
Bossier
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Gde Sasmita Julyantoro
GSJ
Pande
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Vyshal
V
Delahaut
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Ali Md
AM
Rayhan
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Nipa
N
Gupta
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Shikder Saiful
SS
Islam
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Elsie
E
Yumo
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Nancy
N
Nevejan
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Patrick
P
Sorgeloos
CIAD, A.C. Mazatlan Unit for Aquaculture, AP. 711, 82000 Mazatlán, Sinaloa México.
Bruno
B
Gomez-Gil
Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium.|Center for Microbial Ecology and Technology, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Tom
T
Defoirdt
D000818:Animals; D017756:Aquaculture; D049880:Mytilus edulis / Q000382:microbiology*; D014733:Vibrio; D014737:Vibrionaceae / Q000254:growth & development / Q000302:isolation & purification*
Photobacterium; Splendidus; aquaculture; bivalve; immersion challenge; vibriosis