Hen egg yolk phosvitin stimulates osteoblast differentiation in the absence of ascorbic acid.
BACKGROUND
Egg yolk phosvitin, one of the most highly phosphorylated extracellular matrix proteins known in nature, has a strong calcium binding and reducing capacity. Here, we investigated the effects of phosvitin on osteoblast differentiation and osteogenic gene expression in cultured mouse osteoblastic MC3T3-E1 cells by using alkaline phosphatase activity analysis, alizarin red S staining and real-time PCR assay.


RESULTS
Alkaline phosphatase activity and alizarin red S staining analyses demonstrated no significant difference between differentiating MC3T3-E1 cells cultured in the presence of phosvitin and those cultured in the presence of ascorbic acid after 21 days of differentiation. Our real-time PCR assay also indicated the two groups were similar in the expression of the osteogenic gene markers, collagen type I, osteocalcin, runt-related transcription factor 2, and bone morphogenetic protein-2.


CONCLUSIONS
Our findings indicate that phosvitin plays a similar role to that of ascorbic acid in osteoblast differentiation and mineralisation. © 2017 Society of Chemical Industry.
National R27D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
Qingli
http://orcid.org/0000-0001-7684-7118
Q
Liu
National R27D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
Chunyan
C
Li
College of pharmacy and bioengineering, Chengdu University, Chengdu, China.
Fang
F
Geng
National R27D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
Xi
X
Huang
National R27D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
Meihu
M
Ma
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D024042:Collagen Type I / Q000378:metabolism; D004530:Egg Yolk / Q000737:chemistry*; D005260:Female; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010774:Phosvitin / Q000494:pharmacology*
D024042:Collagen Type I; D015675:Osteocalcin; D010774:Phosvitin; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid
ascorbic acid; cell proliferation; egg yolk phosvitin; osteoblast differentation