Effect of Home Cage Bedding in the Induction Chamber on Serum Cortisol and Corticosterone Levels in Response to Isoflurane-induced Anesthesia in C57BL/6J Mice.
Mice are routinely anesthetized with isoflurane in an induction chamber. The AVMA Guidelines for the Euthanasia of Animals states that distress should be minimized during euthanasia but does not address this point in regard to induction of anesthesia. Here we evaluated the potential for familiar surroundings to reduce the adrenocortical response of mice during anesthesia induction with isoflurane. However, adding bedding from the animals' home cage to the induction chamber failed to significantly reduce serum cortisol or corticosterone levels in male and female C57BL/6J mice. These results indicate that familiar surroundings do not appear sufficient to reduce the adrenocortical response of mice during anesthesia induction with isoflurane.
Center for Laboratory Animal Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland;, Email: cara.reiter@usuhs.edu.
Cara P
CP
Reiter
Center for Laboratory Animal Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
Amanda C
AC
Christy
Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
Cara H
CH
Olsen
Center for Laboratory Animal Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
David E
DE
Bentzel
D000758:Anesthesia; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001512:Bedding and Linens; D003345:Corticosterone / Q000097:blood*; D005260:Female; D006799:Housing, Animal; D006854:Hydrocortisone / Q000097:blood*; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013312:Stress, Physiological
D018685:Anesthetics, Inhalation; D007530:Isoflurane; D003345:Corticosterone; D006854:Hydrocortisone