[Genomic analysis of Wenzhou virus in rodents from Zhejiang province].
Objective:Arenavirus is a negative single-stranded RNA virus and an important human pathogen, mainly harbored and transmitted by rodents, causing severe diseases, including hemorrhagic fever and encephalitis. Following the discovery of a novel pathogenic arenavirus (Wenzhou virus, WENV), the prevalence of WENV in local small rodents was investigated.Methods:By using RT-PCR, WENV was screened in 48 and 156 rodents sampled from Wenzhou and Longquan, respectively.Results:Consequently, WENV was detected in 5 (10.41%) rodents sampled from Wenzhou. However, no WENV was identified in all the rodents sampled from Longquan. Genetic analysis of complete genome sequences indicated that 4 of 5 virus strains were closely related to the known Wenzhou viruses with high homology. Especially, the L and S segments of Wencheng-Rn-288 strain shared homology of 87.5% and 91.6% with other viruses, respectively. They formed a distinct lineage, suggesting that this strain might be a novel variant of WENV.Conclusions:Our results indicate that WENV has a high prevalence and high genetic diversity among rodents in Wenzhou. As the respiratory disease caused by WENV has been detected in Cambodia, it is necessary to strengthen the surveillance for WENV in China.
Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
K
K
Li
Institute of Disinfection and Vector Control, Wenzhou Prefecture Center for Disease Control and Prevention, Wenzhou 325000, China.
X D
XD
Lin
Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
M H
MH
Li
Department of Business Management, Longquan County Center for Disease Control and Prevention, Longquan 323700, China.
M R
MR
Wang
Institute of Disinfection and Vector Control, Wenzhou Prefecture Center for Disease Control and Prevention, Wenzhou 325000, China.
X Y
XY
Sun
Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Y Z
YZ
Zhang
D000818:Animals; D018050:Arenavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D002681:China; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D009115:Muridae / Q000821:virology*; D010802:Phylogeny; D015995:Prevalence; D051381:Rats; D012377:Rodentia
Novel variant; Respiratory disease; Rodent; Wenzhou virus