IFN-τDisplays Anti-Inflammatory Effects onStaphylococcus aureusEndometritis via Inhibiting the Activation of the NF-κB and MAPK Pathways in Mice.
The aim of the present study was to determine the anti-inflammatory effect of IFN-τon endometritis using a mouse model ofS. aureus-induced endometritis and to elucidate the mechanism of action underlying these effects. In the present study, the effect of IFN-τonS. aureusgrowth was monitored by turbidimeter at 600 nm. IFN-τdid not affectS. aureusgrowth. The histopathological changes indicated that IFN-τhad a protective effect on uterus tissues withS. aureusinfection. The ELISA and qPCR results showed the production of the proinflammatory cytokines TNF-α, IL-1β, and IL-6 was decreased with IFN-τtreatment. In contrast, the level of the anti-inflammatory cytokine IL-10 was increased. We further studied the signaling pathway associated with these observations, and the qPCR results showed that the expression of TLR2 was repressed by IFN-τ. Furthermore, the western blotting results showed the phosphorylation of IκB, NF-κB p65, and MAPKs (p38, JNK, and ERK) was inhibited by IFN-τtreatment. The results suggested that IFN-τmay be a potential drug for the treatment of uterine infection due toS. aureusor other infectious inflammatory diseases.
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Zhenbiao
Z
Zhang
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Yingfang
Y
Guo
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Yuzhu
Y
Liu
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Chengye
C
Li
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Mengyao
0000-0003-0426-2203
M
Guo
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Ganzhen
0000-0002-5654-4141
G
Deng
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D004195:Disease Models, Animal; D004716:Endometritis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D007370:Interferon Type I / Q000008:administration & dosage* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000096:biosynthesis; D011257:Pregnancy Proteins / Q000008:administration & dosage* / Q000378:metabolism; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000235:genetics / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000472:pathogenicity; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis; D051996:Transcription Factor RelA / Q000096:biosynthesis; D014599:Uterus / Q000187:drug effects / Q000382:microbiology / Q000473:pathology
D000893:Anti-Inflammatory Agents; D007370:Interferon Type I; D016328:NF-kappa B; D011257:Pregnancy Proteins; C498144:Rela protein, mouse; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D051996:Transcription Factor RelA; C020947:interferon tau