Co-localization of proteins with defined sequential order and controlled stoichiometric ratio on magnetic nanoparticles.
Co-immobilization of enzymes used in cascade reactions is important for improving the overall catalytic efficiency. In this work, we employed scaffoldins as a bridge and succeeded in a highly-ordered co-localization of multiple proteins on magnetic nanoparticles with a loading capacity of ∼0.831 μmol g-1supports.
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. fanlh@mail.buct.edu.cn and Beijing Key Laboratory of Bioprocess, Beijing 100029, China.
Yi
Y
Yue
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. fanlh@mail.buct.edu.cn and Beijing Key Laboratory of Bioprocess, Beijing 100029, China.
Yang-Yang
YY
Lu
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. fanlh@mail.buct.edu.cn and Beijing Key Laboratory of Bioprocess, Beijing 100029, China.
Mei
M
Li
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. fanlh@mail.buct.edu.cn and Beijing Key Laboratory of Bioprocess, Beijing 100029, China.
Zi-Jian
ZJ
Zhang
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. fanlh@mail.buct.edu.cn and Beijing Key Laboratory of Bioprocess, Beijing 100029, China.
Tian-Wei
TW
Tan
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. fanlh@mail.buct.edu.cn and Beijing Key Laboratory of Bioprocess, Beijing 100029, China.
Li-Hai
http://orcid.org/0000-0002-6894-9015
LH
Fan