Synthesis and Application of Amine Functionalized Iron Oxide Nanoparticles on Menaquinone-7 Fermentation: A Step towards Process Intensification.
Industrial production of menaquione-7 byBacillus subtilis nattois associated with major drawbacks. To address the current challenges in menaquione-7 fermentation, studying the effect of magnetic nanoparticles on the bacterial cells can open up a new domain for intensified menqainone-7 process. This article introduces the new concept of production and application of l-lysine coated iron oxide nanoparticles (l-Lys@IONs) as a novel tool for menaquinone-7 biosynthesis. l-Lys@IONs with the average size of 7 nm were successfully fabricated and were examined in a fermentation process of l-Lys@IONs decoratedBacillus subtilis natto. Based on the results, higher menaquinone-7 specific yield was observed for l-Lys@IONs decorated bacterial cells as compared to untreated bacteria. In addition, more than 92% removal efficacy was achieved by using integrated magnetic separation process. The present study demonstrates that l-Lys@IONs can be successfully applied during a fermentation of menaquinone-7 without any negative consequences on the culture conditions. This study provides a novel biotechnological application for IONs and their future role in bioprocess intensification.
Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa 74615, Iran. a_ebrahimi@sums.ac.ir.|Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz 71348, Iran. a_ebrahimi@sums.ac.ir.|School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton 3240, New Zealand. a_ebrahimi@sums.ac.ir.
Alireza
A
Ebrahiminezhad
School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton 3240, New Zealand. v_p@hotmail.co.nz.
Vikas
V
Varma
School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton 3240, New Zealand. missshuyi@hotmail.com.
Shuyi
S
Yang
Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz 71348, Iran. ghasemiy@sums.ac.ir.
Younes
Y
Ghasemi
School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton 3240, New Zealand. aydin.berenjian@waikato.ac.nz.
Aydin
A
Berenjian
MK-7; bioseparations; downstream processing; fermentation; immobilization; magnetic nanoparticles