Post-treatment with Ma-Huang-Tang ameliorates cold-warm-cycles induced rat lung injury.
Frequent and drastic ambient temperature variation may cause respiratory diseases such as common cold and pneumonia, the mechanism for which is not fully understood, however, due to lack of appropriate animal models. Ma-Huang-Tang (MHT) is widely used in China for treatment of respiratory diseases. The present study aimed to investigate the effect of MHT on temperature alternation induced rat lung injury and explore underlying mechanisms. Male Sprague-Dawley rats were exposed to a cold environment for 1 h and then shifted to a warm environment for 30 min. This cold and warm alteration cycled 4 times. Rats were administrated with MHT (1.87 g/kg) by gavage 6 h after cold-warm-cycles. Cold-warm-cycles induced pulmonary microcirculatory disorders, lung edema and injury, decrease in the expression of tight junction proteins, increase in VE-cadherin activation, increase in the expression and activation of Caveolin-1, Src and NF-κB, and NADPH oxidase subunits p47phox, p40phoxand p67phoxmembrane translocation and inflammatory cytokines production. All alterations were significantly ameliorated by post-treatment with MHT. This study showed that rats subjected to cold-warm-cycles may be used as an animal model to investigate ambient temperature variation-induced lung injury, and suggested MHT as a potential strategy to combat lung injury induced by temperature variation.
Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.|Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.
Meng-Meng
MM
Xiao
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.
Chun-Shui
CS
Pan
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.
Yu-Ying
YY
Liu
Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.|Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.
Li-Qian
LQ
Ma
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.
Li
L
Yan
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.
Jing-Yu
JY
Fan
Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.|Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.
Chuan-She
CS
Wang
Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.|Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China.
Rong
R
Huang
Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, 100191, China. hanjingyan@bjmu.edu.cn.|Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, 100191, China. hanjingyan@bjmu.edu.cn.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China. hanjingyan@bjmu.edu.cn.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, 100191, China. hanjingyan@bjmu.edu.cn.
Jing-Yan
JY
Han
D000818:Animals; D003080:Cold Temperature; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D004781:Environmental Exposure; D006358:Hot Temperature; D008168:Lung / Q000473:pathology / Q000528:radiation effects; D055370:Lung Injury / Q000473:pathology / Q000517:prevention & control*; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
D004365:Drugs, Chinese Herbal; C493483:Mao-to