Detection of Carbapenemase-Producing Organisms with a Carbapenem-Based Fluorogenic Probe.
Antibiotic resistance has become a major challenge to public health worldwide. This crisis is further aggravated by the increasing emergence of bacterial resistance to carbapenems, typically considered as the antibiotics of last resort, which is mainly due to the production of carbapenem-hydrolyzing carbapenemases in bacteria. Herein, the carbapenem-based fluorogenic probe CB-1 with an unprecedented enamine-BODIPY switch is developed for the detection of carbapenemase activity. This reagent is remarkably specific towards carbapenemases over other prevalent β-lactamases. Furthermore, the efficient imaging of live clinically important carbapenemase-producing organisms (CPOs) with CB-1 demonstrates its potential for the rapid detection of antibiotic resistance and timely diagnosis of CPO infections.
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, P.R. China.
Wuyu
W
Mao
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, P.R. China.
Lingying
L
Xia
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, P.R. China.
Hexin
0000-0003-1033-9211
H
Xie
D000150:Acinetobacter / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D015780:Carbapenems / Q000737:chemistry*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007709:Klebsiella / Q000302:isolation & purification* / Q000378:metabolism; D008968:Molecular Conformation; D011549:Pseudomonas / Q000302:isolation & purification* / Q000378:metabolism; D001618:beta-Lactamases / Q000378:metabolism*
D001426:Bacterial Proteins; D015780:Carbapenems; D005456:Fluorescent Dyes; D001618:beta-Lactamases; C063912:carbapenemase
antibiotic resistance; carbapenemases; fluorescent probes; β-lactamases