Sensitive detection of L-5-hydroxytryptophan based on molecularly imprinted polymers with graphene amplification.
A novel electrochemical sensor was presented for the determination of L-5-hydroxytryptophan (L-5-HTP) based on a graphene-chitosan molecularly imprinted film modified on the surface of glassy carbon electrode (GR-MIP/GCE). The morphology and composition of the imprinted film were observed in field emission scanning electron microscopy (FESEM), raman spectroscopy and fourier transform infrared (FTIR). The properties of the sensor were evaluated by electrochemical techniques. Under the optimal conditions, the peak currents of L-5-HIP were found to be linear in the concentration range of 0.05-7.0 μM, while the sensor also exhibited great features such as low detection limit of 6.0 nM (S/N = 3), superb selectivity against the structural analogues, good antidisturbance ability among coexisting components, excellent repeatability and stability. Moreover, the proposed method had been applied to the detection of L-5-HTP in human blood serum with a satisfactory recoveries ranging from 90.6% to 105.6%.
College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, People's Republic of China.
Lu
L
Chen
College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, People's Republic of China.
Hui-Ting
HT
Lian
College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, People's Republic of China.
Xiang-Ying
XY
Sun
College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, People's Republic of China. Electronic address: bliu@hqu.edu.cn.
Bin
B
Liu
D006916:5-Hydroxytryptophan / Q000097:blood* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D011108:Polymers; D002244:Carbon; D006108:Graphite; D048271:Chitosan; D006916:5-Hydroxytryptophan
Chitosan; Graphene; L-5-Hydroxytryptophan; Molecularly imprinting