Ultra-fast liquid chromatography with tandem mass spectrometry determination of eight bioactive components of Kai-Xin-San in rat plasma and its application to a comparative pharmacokinetic study in normal and Alzheimer's disease rats.
A method of ultra-fast liquid chromatography with tandem mass spectrometry was developed and validated for the simultaneous quantitation of eight bioactive components, including polygalaxanthone III, sibiricaxanthone B, tenuifolin, sibiricose A5, sibiricose A6, tenuifoliside A, ginsenoside Re and ginsenoside Rb1 in rat plasma after oral administration of Kai-Xin-San. The plasma samples were extracted by liquid-liquid extraction using digoxin as an internal standard. Chromatographic separation was performed on a Venusil MP C18column (100 mm × 2.1 mm, 3 μm) with methanol and 0.05% acetic acid in water as mobile phase. The tandem mass spectrometric detection was performed in the multiple reaction monitoring with turbo ion spray source in the negative ionization. Validation parameters were within acceptable ranges. The established method has been successfully applied to compare the pharmacokinetic profiles of the analytes between normal and Alzheimer's disease rats. The results indicated that there were significant differences in pharmacokinetic parameters of some components between two groups, which may be due to the mechanisms of Alzheimer's disease and pharmacological effects of the analytes. The pharmacokinetic research in the pathological state might provide more useful information to guide the clinical usage of herbal medicine.
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Xiaotong
X
Wang
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Yue
Y
Zhang
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Huibin
H
Niu
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Yajing
Y
Geng
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Bing
B
Wang
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Xiaomei
X
Yang
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Pengyu
P
Yan
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Qing
Q
Li
National and Local Joint Engineering Laboratory for Key Technology of Chinese Materia Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
Kaishun
K
Bi
D000818:Animals; D051381:Rats; D000544:Alzheimer Disease / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics; D010949:Plasma / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D012503:Saponins
D004365:Drugs, Chinese Herbal; C000605449:Kai-Xin-San; C407311:tenuifolic saponin; D012503:Saponins
Alzheimer's disease; pharmacokinetics; rat plasma; traditional Chinese medicine; ultra-fast liquid chromatography with tandem mass spectrometry