In Vitro Studies on the Degradability, Bioactivity, and Cell Differentiation of PRP/AZ31B Mg Alloys Composite Scaffold.
In recent years, more and more methods have been developed to improve the bioactivity of the biodegradable materials in bone tissue regeneration. In present study, we used rat mesenchymal stem cells (rMSCs) to evaluate the outcomes of Mg alloys (AZ31B, Magnesium, and Aluminum) and Platelet-rich plasma (PRP)/Mg alloys on rMSCs biocompatibility and osteogenic differentiation. Water absorption experiments indicated that both bare AZ31B and PRP/AZ31B were capable of absorbing large amounts of water. But the water absorption ratio for PRP/AZ31B was significantly higher than that for bare AZ31B. The degradability experiments implied that both samples degraded at same speed. rMSCs on the surface of AZ31B distributed more and better than those on the AZ31B scaffold. In ALP activity experiment, the activity of rMSCs on the PRP/AZ31B was markedly higher than that on the AZ31B scaffolds on the 7th day and 14th day. qRT-PCR also showed that OPN and OCN were expressed in both samples. OPN and OCN expression in PRP/AZ31B sample were higher than those in bare AZ31B samples. In summary, the in vitro study implied that AZ31B combined with PRP could remarkably improve cell seeding, attachment, proliferation, and differentiation.
Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
Jian
J
Zou
Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
Zhongmin
0000-0002-2467-8735
Z
Shi
Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
Hongwei
H
Xu
Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
Xiaolin
X
Li
D020341:Absorbable Implants / Q000009:adverse effects; D000497:Alloys / Q000737:chemistry / Q000627:therapeutic use; D000535:Aluminum / Q000737:chemistry / Q000627:therapeutic use; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008274:Magnesium / Q000737:chemistry / Q000627:therapeutic use; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000096:biosynthesis; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
D000497:Alloys; C586533:Mg-Al-Zn-Mn-Si-Cu alloy; D015675:Osteocalcin; D053495:Osteopontin; D000535:Aluminum; D008274:Magnesium