Interleukin-37 in endometriosis.
Interleukin-37 (IL-37) has been identified as a novel anti-inflammatory cytokine. The present study aimed to evaluate the expression of IL-37 in serum and in peritoneal fluid to determine its clinical significance in endometriosis. Enzyme-linked immunosorbent assay (ELISA) was performed to examine serum IL-37 levels in patients with endometriosis and healthy controls. Peritoneal fluid IL-37 mRNA and NFκB expression were quantified by real-time reverse transcription polymerase chain reaction assays. The association of IL-37 levels with clinical factors and prognosis of endometriosis was analysed. We found that IL-37 levels in PF and in serum were significantly higher in patients with endometriosis compared to women without endometriosis (P=0.0005). IL-37 levels were highly expressed in PF [132.38±34.62pg/mL; P<0.0001] than in serum [74.10±13.49pg/mL] in endometriosis patients. IL-37 mRNA expression contrasted with NFκB mRNA expression in PF from patients with endometriosis. A significant inverse correlation was observed between IL-37 mRNA and NFκB mRNA expression. IL-37 expression correlates with endometriosis severity. The affected NFκB mRNA expression in endometriosis contributed the to exhibited increase of IL-37. The increased levels of IL-37 may dampen NFκB activation in endometriosis patients.
Unit Research 12SP15 "Homeostasis and Cell Dysfunction", A. Mami Hospital, Pavillon B, Ariana, Tunisia; Université de Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia.
Wajih
W
Kaabachi
Unit Research 12SP15 "Homeostasis and Cell Dysfunction", A. Mami Hospital, Pavillon B, Ariana, Tunisia; Université de Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia.
Olfa
O
Kacem
Unit Research 12SP15 "Homeostasis and Cell Dysfunction", A. Mami Hospital, Pavillon B, Ariana, Tunisia; Université de Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia.
Rafik
R
Belhaj
Unit Research 12SP15 "Homeostasis and Cell Dysfunction", A. Mami Hospital, Pavillon B, Ariana, Tunisia; Université de Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia.
Agnes
A
Hamzaoui
Unit Research 12SP15 "Homeostasis and Cell Dysfunction", A. Mami Hospital, Pavillon B, Ariana, Tunisia; Université de Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunis, Tunisia. Electronic address: Kamel.hamzaoui@gmail.com.
Kamel
K
Hamzaoui
D000328:Adult; D001202:Ascitic Fluid / Q000276:immunology*; D004715:Endometriosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000097:blood* / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D055815:Young Adult
C508587:IL37 protein, human; D007375:Interleukin-1; D016328:NF-kappa B; D012333:RNA, Messenger
Endometriosis; IL-17; IL-33; IL-37; Inflammation; NFκB