CyclosporineA augments endoplasmic reticulum stress markers and expression of matrix protein mRNA.
Cyclosporine-A induces gingival overgrowth with disturbance in the homeostasis of cells and connective tissue proteins. Human gingival fibroblasts were cultured with cyclosporine A, and the expression of two vital endoplasmic stress markers and two prime matrix proteins (connective tissue growth factor (CTGF and periostin) were assessed by RT-PCR. We found that expression of Glucose-Regulated Protein 78 (GRP78/BIP) and CCAAT/enhancer binding protein, C/EBP homologous protein (CHOP) were significantly increased, along with CTGF and periostin, suggesting a role for these factors in gingival overgrowth.
Department of Periodontology and Implantology, Faculty of Dental Sciences & Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra University, Chennai, India.
S R
SR
Rao
Centre for Regenerative Medicine and Stem Cell Research, Central Research Facility, Sri Ramachandra University, Chennai, India.
R
R
Subbarayan
Centre for Indian Systems of Medicine Quality Assurance and Standardization, Central Research Facility, Sri Ramachandra University, Chennai, India.
D M
DM
Girija
D015415:Biomarkers / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D016572:Cyclosporine / Q000009:adverse effects*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects* / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D061251:Primary Cell Culture; D015398:Signal Transduction; D051742:Transcription Factor CHOP / Q000819:agonists / Q000235:genetics* / Q000378:metabolism
D015415:Biomarkers; C526317:CCN2 protein, human; D015815:Cell Adhesion Molecules; C497249:DDIT3 protein, human; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D007166:Immunosuppressive Agents; C512835:POSTN protein, human; D055513:Connective Tissue Growth Factor; D051742:Transcription Factor CHOP; D016572:Cyclosporine