The clinicopathologic relevance of RECK gene polymorphisms in ameloblastoma.
OBJECTIVE
To investigate the relationship between RECK gene polymorphisms and the clinicopathologic features of ameloblastoma.


METHODS
Normal gingival mucosa specimens were obtained from 10 healthy volunteers. Ameloblastomas were surgically removed from 30 patients and part of the tumor specimens were used to detect RECK gene polymorphisms by using PCR-single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing analysis. Expression of RECK and MMP-9 protein was measured using western blot.


RESULTS
The overall SNP rate was 46.7% (14/30). Four polymorphisms were detected in exon 9, 11, 13, 15 of the RECK gene: two synonymous (P520P and R625R) and two missense SNPs (V275I and I395V). RECK protein expression in specimens with minor RECK SNPs was lower than that in specimens without RECK SNPs (P<0.05), and, RECK protein expression in specimens with and without RECK SNPs was lower than that in the normal gingiva specimens (P<0.05). MMP-9 protein expression in specimens with minor RECK SNPs was higher than that in specimens without RECK SNPs (P<0.05), and MMP-9 protein expression in specimens with and without RECK SNPs was higher than that in normal gingiva specimens (P<0.05). RECK gene polymorphisms were closely associated with active proliferation, capsular invasion, and clinical recurrence of ameloblastoma.


CONCLUSIONS
The rs16932912(G/A) SNP in the RECK gene was closely associated with active proliferation, capsular invasion, and clinical recurrence of ameloblastoma. RECK protein expression was closely associated with the presence of the rs16932912(G/A) SNP.
Department of Oral & Maxillofacial Surgery, College and Hospital of Stomatology, Hebei Medical University, The Key Laboratory of Stomatology, Hebei Province, China. Electronic address: zxdcrx@163.com.
Xudong
X
Zhang
Department of Oral Pathology, College and Hospital of Stomatology, Hebei Medical University, The Key Laboratory of Stomatology, Hebei Province, China. Electronic address: wangjiephd@163.com.
Jie
J
Wang
Department of Oral Pathology, College and Hospital of Stomatology, Hebei Medical University, The Key Laboratory of Stomatology, Hebei Province, China. Electronic address: lhj2012001@163.com.
Huijuan
H
Liu
Department of Oral Pathology, College and Hospital of Stomatology, Hebei Medical University, The Key Laboratory of Stomatology, Hebei Province, China. Electronic address: yanningzhang@tom.com.
Yanning
Y
Zhang
Department of Oral & Maxillofacial Surgery, College and Hospital of Stomatology, Hebei Medical University, The Key Laboratory of Stomatology, Hebei Province, China. Electronic address: dongfsh@126.com.
Fusheng
F
Dong
D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics* / Q000378:metabolism; D005881:Gingiva / Q000473:pathology; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D014838:Volunteers; D055815:Young Adult
D058851:GPI-Linked Proteins; C115460:RECK protein, human; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
Ameloblastoma; Clinicopathology; Gene polymorphisms; MMP-9; RECK