Introducing crucial protein panel of gastric adenocarcinoma disease.
OBJECTIVE
Since interactome analysis of diseases can provide candidate biomarker panel related to the diseases, in this research, protein-protein interaction (PPI) network analysis is used to introduce the involved crucial proteins in Gastric adenocarcinoma (GA).


BACKGROUND
Gastric adenocarcinoma (GA) is the most common type of stomach cancer. There is no efficient diagnostic molecular method for GA.


METHODS
Applying Cytoscape software 3.4.0 and String Database, the PPI network was constructed for 200 genes. Based on centrality parameters, the critical nodes were screened. Gene ontology of the key proteins for pathway analysis and molecular function processing were done and the highlighted pathways and activities were discussed.


RESULTS
Among 200 initial genes, 141 genes were included in a main connected network. Seven crucial proteins, including tumor protein p53, epidermal growth factor receptor, albumin, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), v-akt murine thymoma viral oncogene homolog 1, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) and catenin (cadherin-associated protein), beta 1, 88kDa, and Myogenic differentiation 1, were introduced as key nodes of the network. These identified proteins are mostly involved in pathways and activities related to cancer.


CONCLUSIONS
In conclusion, the finding is corresponding to the significant roles of these introduced proteins in GA disease. This protein panel may be a useful probe in the management of GA.
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mostafa
M
Rezaei-Tavirani
Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Majid
M
Rezaei-Tavirani
Physiotherapy Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Vahid
V
Mansouri
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Seyed Mohammad
SM
Mahdavi
Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Reza
R
Valizadeh
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad
M
Rostami-Nejad
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad Reza
MR
Zali
Biomarker panel; Gastric adenocarcinoma; Gene ontology; Hub-bottleneck nodes; Protein-protein interaction network