Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes.
The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C21/C42groups (controls); OL21and OL22-42groups (0.5 mL olive oil intake); A1-21groups (3 mL 20% ethanol intake); DSF1-21groups (178.5 mg DSF/kg/day intake); and A21+DSF22-42groups (the DSF ingestion followed previous 21 days' treatment with alcohol). The measured parameters in testes included metals: zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg) and selenium (Se); as well as oxidative stress (OS) parameters: superoxide anion radical (O2•-), glutathione reduced (GSH) and oxidized (GSSG), malondialdehyde (MDA), hydrogen peroxide (H2O2) decomposition and activities of total superoxide dismutase (tSOD), glutathione-S-transferase (GST) and glutathione reductase (GR). Metal status was changed in all experimental groups (Fe rose, Zn fell, while Cu increased in A21+DSF24-32groups). Development of OS was demonstrated in A1-21groups, but not in DSF1-21groups. In A21+DSF22-42groups, OS was partially reduced compared to A groups (A1-21>MDA>C; A1-21<GSH<C). High metal-binding affinity of DSF/DDTC changes red-ox homeostasis in rat testes.
Department for Toxicology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Ana
A
Djuric
Department for Toxicology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Aida
A
Begic
Department for Toxicology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Borko
B
Gobeljic
Department for Applied Chemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
Ana
A
Pantelic
Department for Food Technology, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade-Zemun, Serbia.
Goran
G
Zebic
Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia.
Ivana
I
Stevanovic
Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia.
Dragan
D
Djurdjevic
Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia.
Milica
M
Ninkovic
Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia.
Vera
V
Prokic
Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia.
Ivan
I
Stanojevic
Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia.
Danilo
D
Vojvodic
Department for Toxicology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. Electronic address: mirjana.djukic@pharmacy.bg.ac.rs.
Mirjana
M
Djukic
D000818:Animals; D004221:Disulfiram / Q000009:adverse effects / Q000378:metabolism*; D000431:Ethanol / Q000009:adverse effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013737:Testis / Q000201:enzymology / Q000378:metabolism*
D013481:Superoxides; D000431:Ethanol; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione; D012643:Selenium; D004221:Disulfiram
Alcohol; Disulfiram; Oxidative stress; Testicular toxicity; Transition metals