Taxane-derived compounds protect SK-N-SH cells against oxidative stress injury induced by H2O2.
OBJECTIVE
Discussed the protection of taxane-derived compounds, 7-deacetyl-taxine B and 5-cinnamoyloxy-taxin B, against oxidative stress injury.


METHODS
SK-N-SH cells were pretreated with 7-deacetyl-taxine B, 5-cinnamoyloxy-taxin B or DMSO (control) and then incubated with H2O2for another 24 h. Cell viability was measured by MTT colorimetric assay. Apoptosis rate, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were detected using flow cytometry assay. Ultrastructure was detected by Transmission electron microscope.


RESULTS
Taxane-derived compounds improved H2O2mediated loss of cell viability in SK-N-SH cell. Ultrastructure of control group cells showed nucleus shrinkage, dense aggregation of chromatin, appearance of apoptotic body and mitochondrial dilation. Treated group showed alleviation of mitochondrial dilation and chromatin margination, and reduction of apoptosis cells induced by H2O2. The apoptosis rate was decreased in treated group compared with control group. DCFH-DA staining showed that ROS were significantly decreased in cells treated with taxane-derived compounds compared with control group. Rhodamine123 staining showed that MMP was significantly decreased in cells treated with taxane-derived compounds compared with control group.


CONCLUSIONS
The anti-oxidative stress and neuroprotective effect of taxane-derived compounds was verified here, wherein it could protect neurocytes from H2O2-induced oxidative stress injury.
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Ruisheng
R
Duan
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Xing
X
Xing
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Yachao
Y
Qi
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Nan
N
Yin
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Hongyu
H
Hao
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Hongshan
H
Chu
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Ya
Y
Gao
b Department of Neurology , The Second Hospital of Hebei Medical University , Shijiazhuang , China.
Weiping
W
Wang
a Department of Neurology , Hebei General Hospital , Shijiazhuang , China.
Peiyuan
P
Lv
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D043823:Taxoids / Q000494:pharmacology*
D000975:Antioxidants; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D043823:Taxoids; D006861:Hydrogen Peroxide; D004121:Dimethyl Sulfoxide
Taxane; apoptosis; cell mitochondrial membrane potential; oxidative stress