Focus: The interface between data collection and data processing in cryo-EM.
We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a user-friendly environment to import and manage data recorded by direct electron detectors and perform elemental image processing tasks in a high-throughput manner while new data is being acquired at the microscope. It provides the functionality required to remotely monitor the progress of data collection and data processing, which is essential now that automation in cryo-EM allows a steady flow of images of single particles, two-dimensional crystals, or electron tomography data to be recorded in overnight sessions. The rapid detection of any errors that may occur greatly increases the productivity of recording sessions at the electron microscope.
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
Nikhil
N
Biyani
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
Ricardo D
RD
Righetto
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
Robert
R
McLeod
BioEM Lab, C-CINA, Biozentrum, University of Basel, Basel, Switzerland.
Daniel
D
Caujolle-Bert
BioEM Lab, C-CINA, Biozentrum, University of Basel, Basel, Switzerland.
Daniel
D
Castano-Diez
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
Kenneth N
KN
Goldie
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland. Electronic address: henning.stahlberg@unibas.ch.
Henning
H
Stahlberg
D001331:Automation; D020285:Cryoelectron Microscopy / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D059629:Signal-To-Noise Ratio; D012984:Software; D014584:User-Computer Interface
Automated data collection; Cryo-EM; Drift correction; High throughput image processing