Platelet-derived growth factor A mRNA in platelets is associated with the degree of hepatic fibrosis in chronic hepatitis C.
Transforming growth factor beta 1 (TGFB1) and platelet-derived growth factor (PDGF) are the main cytokines related to hepatic fibrogenesis.
RNA isolated from the platelets and hepatic tissue of 43 HCV carriers was used for quantitative polymerase chain reaction to determine TGFB1, PDGFA, and PDGFB RNA expression.
The mRNA expression of PDGFA in platelets was significantly lower in the group with advanced fibrosis than in the group with early-stage fibrosis. TGFB1 was more frequently expressed in platelets than in hepatic tissue, which was different from PDGFB.
A pathway mediated by overexpression of TGFB1 via PDGFA in megakaryocytes could be involved in the development of fibrosis.
Programa de Pós-Graduação Stricto Sensu em Fisiopatologia em Clínica Médica, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.
Aline Aki
AA
Tanikawa
Laboratório de Biologia Molecular do Hemocentro, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.|Departamento de Bioprocessos e Biotecnologia, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.
Rejane Maria Tommasini
RM
Grotto
Departamento de Clínica Médica, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.
Giovanni Faria
GF
Silva
Laboratório de Biologia Molecular do Hemocentro, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.
Adriana Camargo
AC
Ferrasi
Departamento de Bioprocessos e Biotecnologia, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.
Valéria Cristina Rodrigues
VC
Sarnighausen
Laboratório de Biologia Molecular do Hemocentro, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.|Departamento de Clínica Médica, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brasil.
Maria Inês de Moura Campos
MI
Pardini
D000328:Adult; D001792:Blood Platelets / Q000737:chemistry; D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000150:complications; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000821:virology; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D016133:Polymerase Chain Reaction; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood*; D012333:RNA, Messenger / Q000032:analysis; D012720:Severity of Illness Index; D053773:Transforming Growth Factor beta1 / Q000097:blood*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; C075278:platelet-derived growth factor A