Hyperbaric Oxygen Preconditioning Provides Preliminary Protection Against Doxorubicin Cardiotoxicity.
BACKGROUND
Doxorubicin (DOX) is generally recognized to have important cardiotoxic side effects. Studies are contradictory about the interaction between hyperbaric oxygen (HBO2) therapy and doxorubicin-induced cardiomyotoxicity. Recent data suggests that HBO2therapy can lead to preconditioning of myocardium while generating oxidative stress. Herein we have investigated the effect of HBO2therapy in a DOX-induced cardiomyocyte injury animal model.


METHODS
Twenty-one rats were divided into three equal groups as follows: 1) Group 1 is a control group (without any intervention), used for evaluating the basal cardiac structures and determining the normal value of cardiacs and serum oxidative markers; 2) Group 2 is the doxorubicin group (single dose i.p. 20 mg/kg doxorubicin) for detecting the cardiotoxic and systemic effects of doxorubicin; 3) Group 3 is the doxorubicin and HBO2group (100% oxygen at 2.5 atmospheric for 90 minutes, daily), for evaluating the effect of HBO2in doxorubicin induced cardiotoxicity. At the end of the protocols, the hearts were harvested and blood samples (2 ml) were obtained.


RESULTS
The doxorubicin treated animals (Group 2) had increased oxidative stress markers (both cardiac and serum) and severe cardiac injury as compared to the basal findings in the control group. Nevertheless, the highest cardiac oxidative stress index was detected in Group 3 (control vs. Group 3, p = 0.01). However, histological examination revealed that cardiac structures were well preserved in Group 3 when compared with Group 2.


CONCLUSIONS
Our results suggest that HBO2preconditioning appears to be protective in the doxorubicin-induced cardiotoxicity model. Future studies are required to better elucidate the basis of this preconditioning effect of HBO2.
Department of Cardiovascular Surgery, Medical School of Dicle University.
Orhan
O
Tezcan
Department of Cardiovascular Surgery, Medical School of Dicle University.
Oguz
O
Karahan
Department of Aerospace Medicine, Medoks Hyperbaric Oxygen Treatment Centre.
Mustafa
M
Alan
Department of Histology and Embryology.
Cenap
C
Ekinci
Department of Cardiovascular Surgery, Medical School of Dicle University.
Celal
C
Yavuz
Department of Cardiovascular Surgery, Medical School of Dicle University.
Sinan
S
Demirtas
Department of Biochemistry, Medical School of Dicle University, Diyarbakir, Turkey.
Aysun
A
Ekinci
Department of Cardiovascular Surgery, Medical School of Dicle University.
Ahmet
A
Caliskan
Cardiovascular toxicity; Doxorubicin; Hyperbaric oxygen; Oxidative stress