Yeast aquaporin regulation by 4-hydroxynonenal is implicated in oxidative stress response.
Reactive oxygen species, especially hydrogen peroxide (H2O2), contribute to functional molecular impairment and cellular damage, but also are necessary in normal cellular metabolism, and in low doses play stimulatory role in cell proliferation and stress resistance. In parallel, reactive aldehydes such as 4-hydroxynonenal (HNE), are lipid peroxidation breakdown products which also contribute to regulation of numerous cellular processes. Recently, channeling of H2O2by some mammalian aquaporin isoforms has been reported and suggested to contribute to aquaporin involvement in cancer malignancies, although the mechanism by which these membrane water channels are implicated in oxidative stress is not clear. In this study, two yeast models with increased levels of membrane polyunsaturated fatty acids (PUFAs) and aquaporin AQY1 overexpression, respectively, were used to evaluate their interplay in cell's oxidative status. In particular, the aim of the study was to investigate if HNE accumulation could affect aquaporin function with an outcome in oxidative stress response. The data showed that induction of aquaporin expression by PUFAs results in increased water permeability in yeast membranes and that AQY1 activity is impaired by HNE. Moreover, AQY1 expression increases cellular sensitivity to oxidative stress by facilitating H2O2influx. On the other hand, AQY1 expression has no influence on the cellular antioxidant GSH levels and catalase activity. These results strongly suggest that aquaporins are important players in oxidative stress response and could contribute to regulation of cellular processes by regulation of H2O2influx. © 2017 IUBMB Life, 69(5):355-362, 2017.
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal.|Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal.
Claudia
C
Rodrigues
Rudjer Boskovic Institute, Zagreb, Croatia.
Ivana
I
Tartaro Bujak
Rudjer Boskovic Institute, Zagreb, Croatia.
Branka
B
Mihaljević
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal.|Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal.
Graça
0000-0001-8487-110X
G
Soveral
Rudjer Boskovic Institute, Zagreb, Croatia.
Ana
0000-0003-2000-8950
A
Cipak Gasparovic
D000447:Aldehydes / Q000378:metabolism* / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D002463:Cell Membrane Permeability; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
C115153:AQY1 protein, S cerevisiae; D000447:Aldehydes; D000975:Antioxidants; D020346:Aquaporins; D005231:Fatty Acids, Unsaturated; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; C027576:4-hydroxy-2-nonenal
4-hydroxynonenal (HNE); aquaporins (AQPs); oxidative stress; polyunsaturated fatty acid (PUFA); reactive oxygen species (ROS)