Development of a robust, fast screening method for the potentiometric determination of iodide in urine and salt samples.
In this work, a potentiometric flow injection method is described for the fast bi-parametric determination of iodide and iodate in urine and salt samples. The developed methodology aimed for iodine speciation with a potentially portable system (running on batteries). The iodate reduction to iodide was effectively attained in line within the same manifold. The iodide determination was accomplished in the dynamic range of 2.50×10-6-1.00×10-3M and the total iodine dynamic range, resulted from iodide plus iodate, was 3.50×10-6-2.00×10-3M. The calculated limits of detection were 1.39×10-6M and 1.77×10-6M for iodide and iodate, respectively. A determination rate of 21h-1for the bi-parametric iodide and iodate determination was obtained for sample injection. The urine samples (RSD <5.8% for iodide and RSD <7.0% for iodate) results were in agreement with those obtained by the classic Sandell-Kolthoff reaction colorimetric reference procedure (RD <7.0%) and standard samples from Centers for Disease Control and Prevention, Atlanta, USA (CDC) international inter-laboratory EQUIP program. The developed flow method was also successfully applied to the iodide and iodate determination in salt samples (RSD <3.1% for iodate and iodide), with comparable results to conventional procedures. No significant interferences were observed interference percentage <9% for both determinations.
ICBAS/UP - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, P 4050-313 Porto, Portugal; CIIMAR/CIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos, 4050-208 Matosinhos, Portugal.
Ana
A
Machado
ICBAS/UP - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, P 4050-313 Porto, Portugal; Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal. Electronic address: rbmesquita@icbas.up.pt.
Raquel B R
RBR
Mesquita
ICBAS/UP - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, P 4050-313 Porto, Portugal.
Sara
S
Oliveira
ICBAS/UP - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, P 4050-313 Porto, Portugal; CIIMAR/CIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos, 4050-208 Matosinhos, Portugal.
Adriano A
AA
Bordalo
D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007454:Iodides / Q000032:analysis*; D011199:Potentiometry / Q000379:methods*; D012965:Sodium Chloride / Q000737:chemistry*; D016482:Urinalysis / Q000379:methods*
D007454:Iodides; D012965:Sodium Chloride
Iodide and iodate bi-parametric determination; Potentiometric detection; Salt samples; Urine samples