Upscaled Bioammonium/Ammonia Production by Clostridium Aminophilum Cultured with Soy Protein Isolate.
The goal of this study was to scale up the production of bioammonium/ammonia (BAA) by Clostridium aminophilum from test tube size small batches of 1.0 g of soy protein isolate (SPI) with 10 mL of salt solution to the lab scale bioreactor level of 1.8 kg·18 L-1salt solution in amounts required for precision fertigation in field trials. An 18 L bioreactor was designed and constructed similar to commercially available lab scale bioreactors. Novel methods were implemented to maintain sterility and anaerobic conditions throughout the preparation and operation of the bioreactor. C. aminophilum was cultured at rates of 1.8 kg·18 L-1salt solution, ultimately producing a BAA mean concentration of 82.9 mM, which was 34% greater than the amount achieved in the test tubes of 61.7 mM.
Department of Plant and Environmental Sciences, Horticulture, Clemson Coastal Research and Education Center, Clemson University , Charleston, South Carolina 29414, United States.
Brian K
BK
Ward
Department of Plant and Environmental Sciences, Horticulture, Clemson Coastal Research and Education Center, Clemson University , Charleston, South Carolina 29414, United States.
Robert J
RJ
Dufault
Department of Plant and Environmental Sciences, Horticulture, Clemson Coastal Research and Education Center, Clemson University , Charleston, South Carolina 29414, United States.
Richard
R
Hassell
Department of Plant and Environmental Sciences, Horticulture, Clemson Coastal Research and Education Center, Clemson University , Charleston, South Carolina 29414, United States.
Matthew A
http://orcid.org/0000-0002-3572-2498
MA
Cutulle
bioammonium/ammonia (BAA); bioreactor; hyper-ammonia-producing bacteria (HAP); oxidation reduction potential (ORP); soy protein isolate (SPI)