A quantitative approach for Cd, Cu, Fe and Mn through laser ablation imaging for evaluating the translocation and accumulation of metals in sunflower seeds.
The uptake and accumulation of Cd in sunflower seeds represents an important pathway for imputing potentially toxic metals into human and animal food. In this way, bioimaging of Cd and micronutrients (Cu, Fe and Mn) in the seeds of sunflower grown in soil contaminated with Cd are performed. For this task, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is used in quantitative approach, considering four groups: precursor, control, Cd-low (50mg) and Cd-high (700mg). For attaining our proposals, ICP-MS (nebulizer and auxiliary flow rates and radiofrequency power) and LA (laser intensity, frequency and spot size) parameters were optimized, and the analytical signal improved to 197%, 217%, 232%, and 283%, for57Fe,112Cd,55Mn and63Cu, respectively. The accuracy of proposed method using LA-ICP-MS is evaluated comparing the CRM results (Tomato leaves, NIST SRM 1573a). No difference is found at 95% confidence level. Regarding Cd accumulation in sunflower seeds, the results indicated that cadmium is translocated to seeds, and the cotyledons showed the highest concentration (Cd-high group), ranging from 10 to 20µgg-1. Considering both total concentration and the distribution in the seeds, Cd uptake is responsible to the homeostasis misbalance of micronutrients, which play an essential role in the sunflower metabolism. Such results highlight the importance of bioimaging evaluation, in the translocation and accumulation of metals, contributing to expand the information available of this culture.
Spectrometry, Sample Preparation and Mechanization Group - GEPAM, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil.
Gustavo de S
GS
Pessôa
Department of Chemistry, Federal University of Piauí-UFPI, P.O. Box 64049-550, Teresina, PI, Brazil.
Cícero A
CA
Lopes Júnior
Spectrometry, Sample Preparation and Mechanization Group - GEPAM, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil.
Katherine C
KC
Madrid
Spectrometry, Sample Preparation and Mechanization Group - GEPAM, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil. Electronic address: zezzi@iqm.unicamp.br.
Marco A Z
MAZ
Arruda
D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism; D002138:Calibration; D003300:Copper / Q000378:metabolism; D006368:Helianthus / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D007834:Lasers; D008345:Manganese / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism*; D057054:Molecular Imaging; D012639:Seeds / Q000378:metabolism*
D019216:Metals, Heavy; D002104:Cadmium; D008345:Manganese; D003300:Copper; D007501:Iron
Bioimaging; Cadmium; LA-ICP-MS; Sunflower