New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.
In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
The Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Suedufer 10, Greifswald Insel-Riems 17493, Germany; National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
Mahmoud M
MM
Naguib
Poultry Disease Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Magdy F
MF
El-Kady
Institute of Poultry Diseases, Freie Universität Berlin, 14163 Berlin, Germany.
Dörte
D
Lüschow
Poultry Disease Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Kareem E
KE
Hassan
National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
Abdel-Satar
AS
Arafa
National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
Ali
A
El-Zanaty
National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, P.O. Box 246, Giza 12618, Egypt.
Mohamed K
MK
Hassan
Institute of Poultry Diseases, Freie Universität Berlin, 14163 Berlin, Germany.
Hafez M
HM
Hafez
The Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Suedufer 10, Greifswald Insel-Riems 17493, Germany.
Christian
C
Grund
The Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Suedufer 10, Greifswald Insel-Riems 17493, Germany. Electronic address: timm.harder@fli.de.
Timm C
TC
Harder
D000818:Animals; D002645:Chickens / Q000821:virology*; D060085:Coinfection / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D018352:Coronavirus Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004534:Egypt; D005838:Genotype; D001351:Infectious bronchitis virus / Q000235:genetics / Q000302:isolation & purification*; D009980:Influenza A virus / Q000235:genetics; D005585:Influenza in Birds / Q000150:complications / Q000821:virology; D025202:Molecular Diagnostic Techniques; D009521:Newcastle Disease / Q000150:complications / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics; D011201:Poultry Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D012367:RNA, Viral