Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA.
Both telomere length and telomere G-tail length are altered in human diseases such as cancer and age-related diseases. While most methods for measurement of G-tail and telomere length require electrophoresis, centrifugation, radioisotope labeling and autoradiography, G-tail telomere HPA provides a convenient and useful tool for the examination of G-tail length with a high-throughput platform using genomic DNA or cell lysate. G-tail telomere HPA may be applicable for clinical diagnostics as well as drug target screening.
Department of Cellular and Molecular Biology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. toshi@hiroshima-u.ac.jp.
Hidetoshi
H
Tahara
D004247:DNA / Q000235:genetics; D006801:Humans; D019098:Telomerase / Q000235:genetics; D016615:Telomere / Q000235:genetics*
D004247:DNA; D019098:Telomerase
Aging; Cancer; G-Tail; HPA; hybridization protection assay; High-throughput; Risk assessment