Molecular diagnosis of allergy to Anisakis simplex and Gymnorhynchus gigas fish parasites.
BACKGROUND
There has been an increase in the prevalence of hypersensitivity to Anisakis simplex. There are fish parasites other than Anisakis simplex whose allergenicity has not yet been studied.


OBJECTIVE
To assess IgE hypersensitivity caused by fish parasite allergens in patients with gastro-allergic symptoms after consumption of fish, shellfish or cephalopods, compared with healthy subjects, pollen allergic individuals and children with digestive symptoms after eating marine food.


METHODS
We carried out in vivo tests (skin prick) and in vitro tests (specific IgE determination, Western blot) and component resolved diagnostics (CRD) using microarray analysis in all patients.


RESULTS
CRD better detected sensitisation to allergens from marine parasites than skin prick tests and determination of specific IgE by CAP. Sensitisation to Gymnorhynchus gigas was detected in 26% of patients measured by skin prick tests and 36% measured by IgE.


CONCLUSIONS
The prevalence of hypersensitivity to marine parasite allergens other than Anisakis simplex should be studied, and the most appropriate technique for this is CRD.
Servicio de Alergia, Hospital Universitario Río Hortega, Spain. Electronic address: aliciaarmentia@gmail.com.
A
A
Armentia
Servicio de Gastroenterología, Hospital Universitario Río Hortega, Spain.
J
J
Santos
Servicio de Alergia, Hospital Universitario Río Hortega, Spain.
Z
Z
Serrano
Servicio de Alergia, Hospital Universitario Río Hortega, Spain.
B
B
Martín
Servicio de Pediatría, Hospital Universitario Río Hortega, Spain.
S
S
Martín
Servicio de Gastroenterología, Hospital Universitario Río Hortega, Spain.
J
J
Barrio
Servicio de Pediatría, Hospital Universitario Río Hortega, Spain.
S
S
Fernández
Unidad de apoyo a la Investigación, Hospital Universitario Río Hortega, Spain.
M
M
González-Sagrado
Laboratorios Diater, Madrid, Spain.
F
F
Pineda
Laboratorios Diater, Madrid, Spain.
R
R
Palacios
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D017163:Anisakis / Q000276:immunology; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D002589:Cestoda / Q000276:immunology; D002648:Child; D004435:Eating; D005260:Female; D005393:Fish Diseases / Q000469:parasitology*; D005396:Fish Products / Q000469:parasitology*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000453:epidemiology; D007114:Immunization / Q000706:statistics & numerical data; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D057089:Pathology, Molecular; D015995:Prevalence; D012882:Skin Tests; D055815:Young Adult
D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007073:Immunoglobulin E
Anisakis simplex; Arrays; Component resolved diagnosis; Fish parasite allergens; Gymnorhynchus gigas; Molecular diagnosis