Hepatoprotective effect against CCl4-induced acute liver damage in mice and High-performance liquid chromatography mass spectrometric method for analysis of the constituents of extract of Rubus crataegifolius.
This study is an attempt to evaluate the hepatoprotective activity of Rubus Crataegifolius against carbon tetrachloride-induced liver injury in mice. 70% ethanolic, ethyl acetate and n-BuOH extract of R. crataegifolius were administered daily for 14 days in experimental animals before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. A high-performance liquid chromatography mass spectrometric (HPLC-EIS-MS/MS) method was developed for the determination of the constituents of the extracts. According to the data of HPLC-EIS-MS/MS, the chemical structures of the largely 14 constituents of R. crataegifolius were identified online without time-consuming isolation. Ethyl acetate extracts of R. crataegifolius showed strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl4. According to the data of HPLC-EIS-MS/MS, Oleanic acid, Phlorizin dehydrate and Quercetin-3-rhamnoside are considered as the main hepatoprotective factor in ethyl acetate extract.
a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang , China.
Yongjiao
Y
Sun
a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang , China.
Lingyun
L
Jia
b School of Biological Medicine and Chemical Engineering , Liaoning Institute of Science and Technology , Benxi , PR China.
Zhanbo
Z
Huang
a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang , China.
Jing
J
Wang
a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang , China.
Jincai
J
Lu
a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang , China.
Jing
J
Li
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D010695:Phlorhizin / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D065856:Rubus / Q000737:chemistry*; D012838:Silymarin / Q000494:pharmacology; D053719:Tandem Mass Spectrometry
D010936:Plant Extracts; D020011:Protective Agents; D012838:Silymarin; C012526:quercitrin; D011794:Quercetin; D002251:Carbon Tetrachloride; D010695:Phlorhizin
HPLC-EIS-MS/MS; Hepatoprotective effect; Rubus crataegifolius