Bioaugmentation with hydrolytic microbes to improve the anaerobic biodegradability of lignocellulosic agricultural residues.
Bioaugmentation with hydrolytic microbes was applied to improve the methane yield of bioreactors fed with agricultural wastes. The efficiency of Clostridium thermocellum and Melioribacter roseus to degrade lignocellulosic matter was evaluated in batch and continuously stirred tank reactors (CSTRs). Results from batch assays showed that C. thermocellum enhanced the methane yield by 34%. A similar increase was recorded in CSTR during the bioaugmentation period; however, at steady-state the effect was noticeably lower (7.5%). In contrast, the bioaugmentation with M. roseus did not promote markedly the anaerobic biodegradability, as the methane yield was increased up to 10% in batch and no effect was shown in CSTR. High-throughput 16S rRNA amplicon sequencing was used to assess the effect of bioaugmentation strategies on bacterial and archaeal populations. The microbial analysis revealed that both strains were not markedly resided into biogas microbiome. Additionally, the applied strategies did not alter significantly the microbial communities.
Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark.
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P
Tsapekos
Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark. Electronic address: panak@env.dtu.dk.
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PG
Kougias
Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark; School of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens 15780, Greece.
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SA
Vasileiou
Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark.
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L
Treu
Department of Biology, University of Padua, Via U. Bassi 58/b, 35121 Padova, Italy.
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S
Campanaro
School of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens 15780, Greece.
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G
Lyberatos
Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark.
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I
Angelidaki
D000383:Agriculture; D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis; D064307:Microbiota; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D056804:Biofuels; D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D008031:Lignin; D008697:Methane
Bioaugmentation; Clostridium thermocellum; High throughput sequencing; Melioribacter roseus; Methane yield