Effects of fasting, temperature, and photoperiod on preproghrelin mRNA expression in Chinese perch.
Preproghrelin, a gut/brain peptide, plays an important role in the regulation of food intake and energy homeostasis in teleost and mammals. In the present study, we obtained the full-length preproghrelin cDNA in Chinese perch. The preproghrelin messenger RNA (mRNA) tissue expression showed that level was much higher in stomach and pituitary than in other tissues. The fasting study showed, after gastric emptying (3-6 h), short-term fasting (6-12 h) increased preproghrelin expression in the stomach. While in the pituitary, fasting reduced preproghrelin expression at 1, 3, 12, and 48 h, presenting state fluctuation of self-adjustment. The temperature study showed that the mRNA expression of preproghrelin was the highest in the brain at 26 °C and highest in the stomach at 32 °C, respectively, with different optimum temperature in these two tissues, reflecting spatiotemporal differences of regulation by central nervous system and peripheral organs. The photoperiod study showed that normal light (11 h of lightness and 13 h of darkness) led to highest preproghrelin expression, both in the brain and in the stomach, than continuous light or continuous dark, proving food intake is adapted to natural photoperiod or normal light in this study. These results all indicated that tissue-specific preproghrelin expression of Chinese perch could be significantly affected by environmental factors. Short-term fasting of 6 h after gastric emptying, 26 °C, and normal light led to higher preproghrelin expression, which indicated potential appetite increase in Chinese perch.
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Yi
Y
Song
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Cheng
C
Zhao
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China. xufang_liang@hotmail.com.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China. xufang_liang@hotmail.com.
Xu-Fang
XF
Liang
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Shan
S
He
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Changxu
C
Tian
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Xiaoyan
X
Cheng
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Xiaochen
X
Yuan
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Liyuan
L
Lv
College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.
Wenjie
W
Guo
National Aquafeed Safety Assessment Station, Feed Research Institute, the Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Min
M
Xue
Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, 36849, USA.
Ya-Xiong
YX
Tao
D000818:Animals; D003001:Cloning, Molecular; D005508:Food Deprivation; D005786:Gene Expression Regulation / Q000502:physiology; D054439:Ghrelin / Q000235:genetics / Q000378:metabolism*; D010473:Perciformes / Q000502:physiology*; D017440:Photoperiod; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D054439:Ghrelin; D012333:RNA, Messenger
Chinese perch; Fasting; Photoperiod; Temperature; mRNA expression; preproghrelin