Evaluation of orange peel for biosurfactant production by Bacillus licheniformis and their ability to degrade naphthalene and crude oil.
A Gram-positive bacterium was isolated from mangrove soil and was identified as Bacillus licheniformis (KC710973). The potential of a mangrove microorganism to utilize different natural waste carbon substrates for biosurfactant production and biodegradation of hydrocarbons was evaluated. Among several substrates used in the present study, orange peel was found to be best substrate of biosurfactant yield with 1.796 g/L and emulsification activity of 75.17 % against diesel. Fourier transform infrared spectroscopy analysis of biosurfactant compound revealed that the isolated biosurfactant is in lipopeptide nature. The1H-NMR of the extracted biosurfactant from B. licheniformis has a doublet signal at 0.8-0.9 ppm corresponding to six hydrogen atoms suggests the presence of a terminal isopropyl group. The spectra showed two main regions corresponding to resonance of α-carbon protons (3.5-5.5 ppm) and side-chain protons (0.25-3.0 ppm). All the data suggests that the fatty acid residue is from lipopeptide. From the biodegradation studies, it concluded that the biosurfactant produced by B. licheniformis further can add to its value as an ecofriendly and biodegradable product.
Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University, Tirupati, India. praveen.imb9@gmail.com.|Department of Environmental Engineering, College of Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk, 712-749, South Korea. praveen.imb9@gmail.com.
Arthala Praveen
AP
Kumar
Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University, Tirupati, India.
Avilala
A
Janardhan
Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University, Tirupati, India.|Department of BioNanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do, 461-701, Republic of Korea.
Buddolla
B
Viswanath
Department of Plant Sciences, Hyderabad Central University, Hyderabad, 500046, India.
Kallubai
K
Monika
Department of Environmental Engineering, College of Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk, 712-749, South Korea.
Jin-Young
JY
Jung
Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University, Tirupati, India. gnsimha123@rediffmail.com.
Golla
G
Narasimha
Bacillus licheniformis; Emulsification activity; Lipopeptide; NMR analysis; Orange peels