Short communication: Identification of Corynebacterium bovis by MALDI-mass spectrometry.
Corynebacterium bovis is a mastitis-causing microorganism responsible for economic losses related to decrease in milk production. The aim of the study was identify Corynebacterium spp. strains recovered from milk samples of subclinical mastitis by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Samples were collected during a 10-mo mastitis-monitoring program in a high-production dairy farm. In this study, 80 strains were analyzed; from these 54 (67.5%) were identified at species level as Corynebacterium bovis, 24 (31.2%) isolates were identified at the genus level as Corynebacterium spp., and only 1 (1.35%) isolated had unreliable identification. Results demonstrated that MALDI-MS could be an important technique for the identification of Corynebacterium spp. in milk.
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970, Brazil. Electronic address: hlangoni@fmvz.unesp.br.
Helio
H
Langoni
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970, Brazil.
Carolina Polo
CP
Camargo da Silva
Instituto Federal Catarinense, Rodovia SC-283 Km 08 Fragosos, Concórdia, SC 89703720, Brazil.
Marcella Zampoli
MZ
Troncarelli
Thomson Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, Campinas, SP 13083-859, Brazil.
Alessandra
A
Tata
Thomson Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, Campinas, SP 13083-859, Brazil.
Katia Roberta Anacleto
KRA
Belaz
Thomson Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, Campinas, SP 13083-859, Brazil.
Marcos Nogueira
MN
Eberlin
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970, Brazil.
Samea Fernandes
SF
Joaquim
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970, Brazil.
Felipe Freitas
FF
Guimarães
Faculdade de Tecnologia de Marília, Av. Castro Alves, 62 - Somenzari, Marília SP, 17500-000, Brazil.
Renata Bonini
RB
Pardo
Departamento de Engenharia Agronômica e Engenharia de Pesca, Universidade Estadual Paulista, Campus de Registro, Avenida Nelson Brihi Badur, 430 - Vila Tupy, Registro SP, 11900-000, Brazil.
Eduardo Nardini
EN
Gomes
D000818:Animals; D002417:Cattle; D003352:Corynebacterium / Q000302:isolation & purification*; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary*
Corynebacterium bovis; mastitis; proteomic characterization