Allele frequencies and other forensic parameters of the HID-Ion AmpliSeq™ Identity Panel markers in Basques using the Ion Torrent PGM™ platform.
The HID-Ion AmpliSeq™ Identity Panel amplifies 90 autosomal SNPs and 34 Y- SNPs with massively parallel sequencing (MPS) using the Ion Torrent PGM™ platform. In the present study, 105 Basques were analyzed to assess this panel. All loci were in Hardy-Weinberg equilibrium and no association between them was detected. Forensic parameters were calculated as 5.74×10-36for combined match probability and 99.99998% for combined power of exclusion. In conclusion, the HID Identity panel and the use of this new MPS technology are very promising tools for paternity testing and human identification in routine casework in the forensic field.
Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain. Electronic address: gobies01@euskalnet.net.
O
O
García
Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.
A
A
Soto
Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.
I
I
Yurrebaso
D016172:DNA Fingerprinting; D005787:Gene Frequency; D005828:Genetics, Population; D006239:Haplotypes; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation*; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D013030:Spain
Basques; HID identity panel; Human identification; Ion torrent PGM™; Massively parallel sequencing (MPS); Population genetics