Development and nitrate reductase activity of sugarcane inoculated with five diazotrophic strains.
Diazotrophs are able to stimulate plant growth. This study aimed at evaluating the effect of inoculation of five diazotrophic strains on growth promotion and nitrate reductase (NR, EC 1.7.1.1) activity in sugarcane. An experiment was carried out from three stages of cultivation: sprouting, tubes, and in hydroponics. On the first two stages, seven treatments were adopted: uninoculated control; mixed inoculation with five strains; and individual inoculation with Gluconacetobacter diazotrophicus (Gd), Herbaspirillum rubrisubalbicans (Hr), Herbaspirillum seropedicae (Hs), Nitrospirillum amazonense (Na), and Paraburkholderia tropica (Pt). The four treatments showing the best performance were transferred to the hydroponic system for analysis of NR activity. Hs, Pt, and the mixture of all strains led to the highest seedling biomass in tubes, followed by Hr. In hydroponics, the mixture and the strain Hr had the highest growth-promoting effect. NR activity was influenced by inoculation only under low N supply conditions, with positive effect of Hr, Pt, and the mixture.
Universidade Federal Rural do Rio de Janeiro, Km 07, BR 465, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Silvana Gomes
SG
Dos Santos
Universidade Federal Rural do Rio de Janeiro, Km 07, BR 465, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Flaviane
F
da Silva Ribeiro
Embrapa Agrobiologia, Km 07, BR 465, Seropédica, Rio de Janeiro, 23891-000, Brazil.
Camila Sousa
CS
da Fonseca
Universidade Federal Rural do Rio de Janeiro, Km 07, BR 465, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Willian
W
Pereira
Universidade Federal Rural do Rio de Janeiro, Km 07, BR 465, Seropédica, Rio de Janeiro, 23890-000, Brazil.
Leandro Azevedo
LA
Santos
Embrapa Agrobiologia, Km 07, BR 465, Seropédica, Rio de Janeiro, 23891-000, Brazil. veronica.massena@embrapa.br.
Veronica Massena
http://orcid.org/0000-0003-2149-7725
VM
Reis
D059827:Agricultural Inoculants / Q000502:physiology*; D042521:Burkholderiaceae / Q000502:physiology*; D041821:Gluconacetobacter / Q000502:physiology*; D042803:Herbaspirillum / Q000502:physiology*; D050901:Nitrate Reductase / Q000378:metabolism*; D009586:Nitrogen Fixation; D010940:Plant Proteins / Q000378:metabolism*; D012244:Rhodospirillaceae / Q000502:physiology*; D031786:Saccharum / Q000201:enzymology / Q000254:growth & development / Q000382:microbiology*
D010940:Plant Proteins; D050901:Nitrate Reductase
Diazotrophs; Growth promotion; Nitrogen metabolism-related enzymes; Saccharum sp