CLDN-1 promoted the epithelial to migration and mesenchymal transition (EMT) in human bronchial epithelial cells via Notch pathway.
Claudin-1 (CLDN-1) is one of main tight junction components that play an important role in epithelial-mesenchymal transition (EMT). However, the effects of CLDN-1 on the migration and EMT induced by TGF-β1 in primary normal human bronchial epithelial (NHBE) and BEAS-2B cells have not been clear. The expression of CLDN-1 was quantified by Western blotting in NHBE and BEAS-2B cells. Cell migration and invasion were detected using transwell assays. The expression level of E-cadherin, N-cadherin, α-SMA, and Vimentin was evaluated by quantitative real-time PCR and Western blotting. Here we showed that the protein expression of CLDN-1 was increased exposed to TGF-β1 in a dose- and time-dependent manner. Knockdown of CLDN-1 using small interfering CLDN-1 RNA (siCLDN-1) prevented the migration and invasion in NHBE and BEAS-2B cells. Moreover, depletion of CLDN-1 promoted the E-cadherin expression and decreased the mRNA and protein levels of N-cadherin, α-SMA, and Vimentin induced by TGF-β1. Furthermore, CLDN-1 silencing resulted in the reduction of the Notch intracellular domain (NICD) and hairy enhancer of split-1 (Hes-1) in mRNA and protein level. Jagged-1, an activator of Notch signaling pathway, abrogated the protective function of siCLDN-1 in migration and EMT. In conclusion, CLDN-1 promoted the migration and EMT through the Notch signaling pathway.
Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, People's Republic of China.
Jing
J
Lv
Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, People's Republic of China. baohuas_cz@163.com.
Baohua
B
Sun
Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, People's Republic of China.
Zhitao
Z
Mai
Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, People's Republic of China.
Mingming
M
Jiang
Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, People's Republic of China.
Junfeng
J
Du
D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement; D062445:Claudin-1 / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction
C568108:CLDN1 protein, human; D062445:Claudin-1; D051880:Receptors, Notch
Claudin-1 (CLDN-1); Epithelial–mesenchymal transition (EMT); Migration and invasion; Notch pathway