Augmented oxidative stress in infertile women with persistent chlamydial infection.
There is established association between oxidative stress, infections of genital tract and fertility. Genital tract infections may provoke increased production of free radicals and generate oxidative stress that can be involved in pathophysiology of a number of reproductive diseases and complications during pregnancy. The aim of this study was to determine connection between oxidative stress and infertility associated with persistent chlamydial infection. Serum samples of infertile women with tubal factor infertility (TFI), women with multiple spontaneous abortions (MSA) and fertile women was screened for C. trachomatis MOMP specific IgG and IgA antibodies and cHSP60 specific igG antibodies using ELISA. The levels of superoxide anion radical, nitric oxide and reduced glutathione were determined spectrophotometricaly. Serum levels of testosterone, luteinizing hormone and follicle stimulating hormone were determined by enzyme-linked fluorescent immunoassay method. Our results showed that persistent infection was more prevalent in TFI than in MSA group, whereas seropositivity was higher in MSA than in TFI group of patients. We also found that superoxide anion was significantly lower, while LH was markedly higher in TFI and MSA group of patients. However, when our results were analyzed according to the serological status of chlamydial infection, we found that parameters of oxidative stress, superoxide anion and index of oxidative stress, defined as relative ratio between superoxide anion and nitrites sum and glutathione ((O2-+NO2-)/GSH) were significantly elevated in infertile patients with persistent chlamydial infection compared to seropositive and seronegative patients. Our findings point to the possible impact of Chlamydia trachomatis infection on prooxidative-antioxidative balance that can influence fertility potential in women with persistent chlamydial infection.
Doctoral Academic Study, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia.
Jelena
J
Tošić-Pajić
Department for Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
Dragana
D
Šeklić
Department for Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
Jelena
J
Radenković
Department for Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Serbia.
Snežana
S
Marković
Public Health Institute Kragujevac, Nikole Pasica 1, 34000 Kragujevac, Serbia.
Jelena
J
Čukić
Public Health Institute Kragujevac, Nikole Pasica 1, 34000 Kragujevac, Serbia; Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. Electronic address: dejan.baskic@gmail.com.
Dejan
D
Baskić
Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia.
Suzana
S
Popović
Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia.
Milos
M
Todorović
Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia; Department of Gynecology and Obstetrics, Clinical Center Kragujevac, Zmaj Jovina 30, 34000 Kragujevac, Serbia.
Predrag
P
Sazdanović
D000026:Abortion, Habitual / Q000382:microbiology; D000328:Adult; D018834:Chaperonin 60 / Q000378:metabolism; D002690:Chlamydia Infections / Q000473:pathology*; D002692:Chlamydia trachomatis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005184:Fallopian Tube Diseases / Q000382:microbiology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007247:Infertility, Female; D024101:Mitochondrial Proteins / Q000378:metabolism; D018384:Oxidative Stress
D018834:Chaperonin 60; C060293:HSPD1 protein, human; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D024101:Mitochondrial Proteins
Oxidative stress; Persistant chlamidial infection; Tubal factor infertility