Mathematical Model of Serodiagnostic Immunochromatographic Assay.
This article describes the mathematical model for an immunochromatographic assay for the detection of specific immunoglobulins against a target antigen (antibodies) in blood/serum (serodiagnosis). The model utilizes an analytical (non-numerical) approach and allows the calculation of the kinetics of immune complexes' formation in a continuous-flow system using commonly available software, such as Microsoft Excel. The developed model could identify the nature of the influence of immunochemical interaction constants and reagent concentrations on the kinetics of the formation of the detected target complex. On the basis of the model, recommendations are developed to decrease the detection limit for an immunochromatographic assay of specific immunoglobulins.
A.N. Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences , Leninsky Prospect 33, Moscow 119071, Russia.
Dmitriy V
0000-0002-4993-0685
DV
Sotnikov
A.N. Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences , Leninsky Prospect 33, Moscow 119071, Russia.
Anatoly V
AV
Zherdev
A.N. Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences , Leninsky Prospect 33, Moscow 119071, Russia.
Boris B
BB
Dzantiev
D007118:Immunoassay; D007136:Immunoglobulins / Q000097:blood*; D007700:Kinetics; D008962:Models, Theoretical
D007136:Immunoglobulins