Whole-genome sequencing of Bacillus velezensis LS69, a strain with a broad inhibitory spectrum against pathogenic bacteria.
Bacillus velezensis LS69 was found to exhibit antagonistic activity against a diverse spectrum of pathogenic bacteria. It has one circular chromosome of 3,917,761bp with 3,643 open reading frames. Genome analysis identified ten gene clusters involved in nonribosomal synthesis of polyketides (macrolactin, bacillaene and difficidin), lipopeptides (surfactin, fengycin, bacilysin and iturin A) and bacteriocins (amylolysin and amylocyclicin). In addition, B. velezensis LS69 was found to contain a series of genes involved in enhancing plant growth and triggering plant immunity. Whole genome sequencing of Bacillus velezensis LS69 will provide a basis for elucidation of its biocontrol mechanisms and facilitate its applications in the future.
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
Guoqiang
G
Liu
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
Yingying
Y
Kong
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
Yajing
Y
Fan
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
Ce
C
Geng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
Donghai
D
Peng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China. Electronic address: m98sun@mail.hzau.edu.cn.
Ming
M
Sun
D000900:Anti-Bacterial Agents / Q000378:metabolism; D001407:Bacillus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D010937:Plant Growth Regulators / Q000378:metabolism; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D010937:Plant Growth Regulators
Antimicrobial metabolites; Bacillaene (PubChem CID: 44227768); Bacillibactin (PubChem CID: 125349); Bacillus velezensis LS69; Bacilysin (PubChem CID: 86583338); Difficidin (PubChem CID: 6438594); Fengycin (PubChem CID: 62705048); Iturin (PubChem CID: 102287549); Macrolactin (PubChem CID: 6451096); Plant growth promotion; Surfactin (PubChem CID: 443592)