HOTAIR functions as a competing endogenous RNA to regulate PTEN expression by inhibiting miR-19 in cardiac hypertrophy.
Sustained cardiac hypertrophy (CH) is related to a variety of physiological as well as pathological stimuli and eventually increases the risk of heart failure. HOTAIR has been identified as a competing endogenous RNA in multiple human biological processes. Whether lncRNA-HOTAIR is involved in the progress of CH and how it works still remain unknown. Herein, we found that HOTAIR was down-regulated, while miR-19 was up-regulated in both heart tissues from TAC-operated mice in vivo and cultural cardiomyocytes treated with Ang-II in vitro by real-time PCR. Meanwhile, HOTAIR expression was negatively correlated with miR-19 in TAC-operated mice. HOTAIR overexpression reduced cell surface area and the expression of hypertrophic markers ANP, BNP, and β-MHC in response to Ang-II stimulation as well as knockdown of miR-19. The further molecular mechanisms of HOTAIR action in CH demonstrated that HOTAIR may act as a competing endogenous RNA (ceRNA) for miR-19, thereby modulating the dis-inhibition of its endogenous target PTEN and playing an important role in inhibiting CH progress. These findings reveal a novel function of LncRNAs, which conduce to an extensive understanding of CH and provide novel research directions and therapeutic options for treating this disease.
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China.
Yanjun
Y
Lai
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China.
Shuai
S
He
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China. lmma_xa@21cn.com.
Liming
L
Ma
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China.
Hong
H
Lin
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China.
Biyun
B
Ren
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China.
Jing
J
Ma
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China.
Xinyu
X
Zhu
Department of Clinical Laboratory, Ninth Hospital of Xi'an, 151 East of South Second Ring Road, Xi'an, 710003, Shaanxi, China.
Shifang
S
Zhuang
D000818:Animals; D006332:Cardiomegaly / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis* / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*
C000608169:HOTAIR long non-coding RNA, mouse; C561642:MIRN19 microRNA, mouse; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse
Cardiac hypertrophy; Competing endogenous RNA; HOTAIR; MiR-19; PTEN