An Extract from Shrimp Processing By-Products Protects SH-SY5Y Cells from Neurotoxicity Induced by Aβ25-35.
Increased evidence suggests that marine unsaturated fatty acids (FAs) can protect neurons from amyloid-β (Aβ)-induced neurodegeneration. Nuclear magnetic resonance (NMR), high performance liquid chromatography (HPLC) and gas chromatography (GC) assays showed that the acetone extract 4-2A obtained from shrimpPandalus borealisindustry processing wastes contained 67.19% monounsaturated FAs and 16.84% polyunsaturated FAs. The present study evaluated the anti-oxidative and anti-inflammatory effects of 4-2A in Aβ25-35-insulted differentiated SH-SY5Y cells. Cell viability and cytotoxicity were measured by using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Quantitative PCR and Western blotting were used to study the expression of neurotrophins, pro-inflammatory cytokines and apoptosis-related genes. Administration of 20 μM Aβ25-35significantly reduced SH-SY5Y cell viability, the expression of nerve growth factor (NGF) and its tyrosine kinase TrkA receptor, as well as the level of glutathione, while increased reactive oxygen species (ROS), nitric oxide, tumor necrosis factor (TNF)-α, brain derived neurotrophic factor (BDNF) and its TrkB receptor. Aβ25-35also increased the Bax/Bcl-2 ratio and Caspase-3 expression. Treatment with 4-2A significantly attenuated the Aβ25-35-induced changes in cell viability, ROS, GSH, NGF, TrkA, TNF-α, the Bax/Bcl-2 ratio and Caspase-3, except for nitric oxide, BDNF and TrKB. In conclusion, 4-2A effectively protected SH-SY5Y cells against Aβ-induced neuronal apoptosis/death by suppressing inflammation and oxidative stress and up-regulating NGF and TrKA expression.
Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. zhangyp2015@163.com.|Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. zhangyp2015@163.com.
Yongping
Y
Zhang
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. sebrina2006@gmail.com.|Aquatic and Crop Resource Development, National Research Council of Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. sebrina2006@gmail.com.
Guangling
G
Jiao
Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. cai.song@dal.ca.|Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. cai.song@dal.ca.|Graduate Institute of Neural and Cognitive Sciences, China Medical University Hospital, Taichung 40402, Taiwan. cai.song@dal.ca.
Cai
C
Song
Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. xiaomei.gu@dal.ca.
Shelly
S
Gu
Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. Richard.Brown@dal.ca.
Richard E
RE
Brown
Aquatic and Crop Resource Development, National Research Council of Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Junzeng.Zhang@nrc-cnrc.gc.ca.
Junzeng
J
Zhang
Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. pczhang2000@yahoo.com.
Pingcheng
P
Zhang
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. jacques.gagnon@umoncton.ca.
Jacques
J
Gagnon
Aquatic and Crop Resource Development, National Research Council of Canada, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada. Steven.Locke@nrc-cnrc.gc.ca.
Steven
S
Locke
Aquatic and Crop Resource Development, National Research Council of Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Roumiana.Stefanova@nrc-cnrc.gc.ca.
Roumiana
R
Stefanova
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada. Claude.pelletier@umoncton.ca.
Claude
C
Pelletier
Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. hubeizhangyi@163.com.
Yi
Y
Zhang
Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. irislhy@126.com.
Hongyu
H
Lu
D016229:Amyloid beta-Peptides / Q000009:adverse effects* / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003445:Crustacea / Q000737:chemistry*; D019610:Cytoprotection / Q000187:drug effects; D006801:Humans; D009447:Neuroblastoma / Q000188:drug therapy; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000737:chemistry* / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000188:drug therapy* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000009:adverse effects* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016229:Amyloid beta-Peptides; D019208:Brain-Derived Neurotrophic Factor; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C074636:amyloid beta-protein (25-35); C579455:BDNF protein, human; D053148:Caspase 3
Acetone extract from shrimp processing by-product (4-2A); Alzheimer’s disease (AD); Aβ25–35; human neuroblastoma cell (SH-SY5Y); neuroprotection; polyunsaturated fatty acids