Cells capture and antimicrobial effect of hydrophobically modified chitosan coating on Escherichia coli.
Hydrophobically modified chitosan (HMCS), prepared by reacting alkyl aldehyde with chitosan was demonstrated to be an effective antimicrobial and transparent coating. The grafted alkyl chains exist as protruded hydrophobic tails on the HMCS coating surface. In contact with E. coli cells, HMCS coating captured and immobilized the cells via these hydrophobic tails. The hydrophobic tails could also kill the cells captured on the coating surface as visualized by fluorescence microscope. More than 50% of the initially loaded cells (2.5×104CFU) could be killed after 2h contact with HMCS coating. The cells capture and killing effects of the coating surface could be completely neutralized by treating with α-cyclodextrin to sequester the protruded hydrophobic tails. The facile coating of antimicrobial HMCS on surface also enabled the easy fabrication of patterned E. coli cells arrays.
Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Rd. Sec.4, Taipei 106, Taiwan. Electronic address: vdthangbk@gmail.com.
Duc-Thang
DT
Vo
Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Rd. Sec.4, Taipei 106, Taiwan. Electronic address: cklee@mail.ntust.edu.tw.
Cheng-Kang
CK
Lee
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects*; D057927:Hydrophobic and Hydrophilic Interactions
D000900:Anti-Bacterial Agents; D048271:Chitosan
Antimicrobial surface; Cell immobilization; Cell patterning; Hydrophobically modified chitosan