Lipopolysaccharide-induced suppression of periodontal ligament cell proliferation and apoptosis are strengthened under high glucose conditions.
The present study aimed to investigate the effect of lipopolysaccharide (LPS) on the proliferation and apoptosis of human periodontal ligament (hPDL) cells under normal glucose or high glucose conditions. Primary cultures of hPDL cells were prepared from extracted premolars of patients. The cells were incubated with 0, 1, or 10μg/mL LPS under normal glucose (5.5mmol/L) or high glucose (25mmol/L) conditions for 24h or 48h. Cell proliferation was detected using a CCK-8 assay, and cell apoptosis was measured by Hoechst 33258 staining and Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining. BCL2 and BAX mRNA and protein levels were measured by real-time polymerase chain reaction and western blotting, respectively. LPS (10μg/mL) induced significant inhibition of cell proliferation and cell apoptosis, and a significant decrease in the BCL2/BAX ratio in the cells cultured with 5.5mmol/L glucose. These effects of LPS were increased significantly in cells treated with 25mmol/L glucose. Analysis of variance of the factorial design revealed that high glucose and LPS had a significant interaction for cell apoptosis, but not for cell proliferation. High glucose augmented LPS-induced hPDL cell apoptosis and cell proliferation inhibition. LPS and high glucose might interact to induce cell apoptosis.
Department of Reproductive Medicine, Affiliated Hospital of Qingdao University, Qingdao 266000, China.
Jianxin
J
Liu
Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, China.
Shulan
S
Chen
Pediatric dentistry, Dongfeng Stomatological Hospital Affiliated Hubei University of Medicine, China.
Weiwei
W
Ren
Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, China.
Jianing
J
Liu
Department of Periodontology, and Key Laboratory of Oral Biomedicine of Shandong Province, School of Stomatology, Shandong University,44-1 Wenhua Road West, 250012 Jinan, China. Electronic address: yangpishan_yps@hotmail.com.
Pishan
P
Yang
Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, China.
Zhenggang
Z
Chen
Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, China.
Qiang
Q
Zhang
Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, China.
Fang
F
Yang
D000293:Adolescent; D000704:Analysis of Variance; D017304:Annexin A5; D017209:Apoptosis / Q000187:drug effects*; D001641:Bicuspid; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002648:Child; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects*; D011419:Propidium; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D017304:Annexin A5; C494826:BAX protein, human; C000595707:BCL2 protein, human; C492760:FITC-annexin A5; D008070:Lipopolysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D011419:Propidium; D016650:Fluorescein-5-isothiocyanate; D005947:Glucose
BAX; BCL2; Cell apoptosis; High glucose; Lipopolysaccharide