Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA.
Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.
Chenguang
C
Lou
Simone V
SV
Samuelsen
Department of Chemistry, Biomolecular Nanoscale Engineering Center, University of Copenhagen , Thorvaldsensvej 40, Frederiksberg 1871, Denmark.
Niels Johan
NJ
Christensen
Birte
B
Vester
Jesper
0000-0001-9835-1009
J
Wengel
D018076:DNA, Complementary / Q000378:metabolism; D003851:Deoxyribonucleases; D004351:Drug Resistance; D004355:Drug Stability; D008958:Models, Molecular; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D018075:RNA, Complementary / Q000378:metabolism; D055672:Static Electricity
D018076:DNA, Complementary; D009841:Oligonucleotides; D018075:RNA, Complementary; C527207:amino-LNA; D003851:Deoxyribonucleases