Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile.
Sertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50μM, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs). While, the highest concentration of EGCG (50μM) increased glucose and pyruvate consumption, it decreased the conversion of pyruvate to alanine to sustain a regular lactate production. However, despite maintaining Krebs cycle functionality, EGCG (50μM) decreased mitochondrial membrane potential of hSCs, which could compromise the normal rates of ATP production. Interestingly, oxidative damages to proteins and lipids decreased in this experimental group, which may be valuable for the nutritional support of spermatogenesis.
Centro de Investigação em Ciências da Saúde (CICS-UBI), Universidade da Beira Interior, 6201-001 Covilhã, Portugal; Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar, Unit for Multidisciplinary Research in Biomedicine, University of Porto, 4050-313 Porto, Portugal; LAQV/REQUIMTE - Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, 4150-755 Porto, Portugal.
Tânia R
TR
Dias
Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar, Unit for Multidisciplinary Research in Biomedicine, University of Porto, 4050-313 Porto, Portugal.
Marco G
MG
Alves
Centre for Reproductive Genetics Prof. Alberto Barros, 4100-009, Porto, Portugal.
Joaquina
J
Silva
Centre for Reproductive Genetics Prof. Alberto Barros, 4100-009, Porto, Portugal; Department of Genetics, Faculty of Medicine, University of Porto, 4050-313 Porto, Portugal; i3S- Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.
Alberto
A
Barros
Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar, Unit for Multidisciplinary Research in Biomedicine, University of Porto, 4050-313 Porto, Portugal; Centre for Reproductive Genetics Prof. Alberto Barros, 4100-009, Porto, Portugal.
Mário
M
Sousa
LAQV/REQUIMTE - Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, 4150-755 Porto, Portugal.
Susana
S
Casal
Centro de Investigação em Ciências da Saúde (CICS-UBI), Universidade da Beira Interior, 6201-001 Covilhã, Portugal. Electronic address: bmcms@ubi.pt.
Branca M
BM
Silva
Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar, Unit for Multidisciplinary Research in Biomedicine, University of Porto, 4050-313 Porto, Portugal; Department of Genetics, Faculty of Medicine, University of Porto, 4050-313 Porto, Portugal; i3S- Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal. Electronic address: pfobox@gmail.com.
Pedro F
PF
Oliveira
D000409:Alanine / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D051273:Glucose Transporter Type 2 / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D019289:Pyruvic Acid / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism
D051273:Glucose Transporter Type 2; D027501:Monocarboxylic Acid Transporters; D009124:Muscle Proteins; C406962:SLC16A4 protein, human; C495680:SLC2A2 protein, human; D019289:Pyruvic Acid; D002392:Catechin; C045651:epigallocatechin gallate; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D000409:Alanine
Epigallocatechin-3-gallate; Glucose metabolism; Human Sertoli cells; Mitochondrial bioenergetics; Oxidative profile; Spermatogenesis