DNA integrity-protecting and survival-promoting activity of serotonergic system in sturgeon juveniles and sazans.
The study concerns evaluation of engagement of serotonergic system in the mechanisms of antimutagenic protection and survival under adverse conditions. It is shown that under long-term exposure to oil and industrial pollution, simultaneous sharp increase of mutations level in the erythrocytes and downregulation of serotonin-modulating anticonsolidation protein (SMAP; Mekhtiev 2000) in the livers of the sturgeon juveniles (Acipenser gueldenstaedtii persicus) is observed. Mutation level was evaluated by the micronucleus analysis and SMAP level-by the indirect ELISA-test utilizing anti-SMAP immunoglobulins. Intramuscular administration of SMAP leads to significant decrease of micronucleus amount in the erythrocytes of the sturgeon juveniles exposed to sediments polluted with PAH and heavy metals. Pre-conditioning of sazans by low dose of insecticide actara (100 mg/l) leads to upregulation of SMAP and to survival of all the animals in the experimental group, kept under high concentration of actara (400 mg/l), while all animals in the control group succumbed under these conditions. I.m. administration of SMAP prior to putting the sazans into the water containing high levels of actara (400 mg/l), in contrast to controls, leads to their total survival. Participation of serotonergic system in the mechanisms of antimutagenic protection and survival promotion under damaging conditions is concluded.
Department of Molecular Basis of Integrative Activity, Karaev Institute of Physiology, 78 Sharif-zade St, AZ1100, Baku, Azerbaijan. arifmekht@yahoo.com.
Arif A
AA
Mekhtiev
Department of Molecular Basis of Integrative Activity, Karaev Institute of Physiology, 78 Sharif-zade St, AZ1100, Baku, Azerbaijan.|Ministry of Agriculture, Azerbaijan State Agrarian University, 262 Ataturk Ave., AZ2000, Ganja, Azerbaijan.
Turan N
TN
Allahverdiyeva
Department of Molecular Basis of Integrative Activity, Karaev Institute of Physiology, 78 Sharif-zade St, AZ1100, Baku, Azerbaijan.|Department of Ecotoxicology, Karaev Institute of Physiology, 78 Sharif-zade St, AZ1100, Baku, Azerbaijan.
Sevda K
SK
Movsum-Zadeh
D000818:Animals; D004249:DNA Damage / Q000502:physiology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005399:Fishes / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008136:Longevity / Q000502:physiology*; D009154:Mutation; D010578:Petroleum / Q000633:toxicity; D012701:Serotonin / Q000378:metabolism*; D014874:Water Pollutants, Chemical
D029941:Fish Proteins; D010578:Petroleum; D014874:Water Pollutants, Chemical; D012701:Serotonin
Adverse environmental factors; Antimutagenic protection; Mutations; Serotonin-modulating anticonsolidation protein; Survival promotion