Response of Catalase of the Mouse Primary Hepatocytes to Sodium Dodecylbenzenesulfonate and the Underlying Molecular Mechanisms.
This study investigated the adverse effects of sodium dodecylbenzenesulfonates (SDBS) on mouse primary hepatocytes by conducting cell viability, intracellular oxidative stress level, and catalase (CAT) activity assays. It was shown that SDBS altered CAT activities, triggered oxidative stress, and thus exhibited cytotoxicity to the hepatocytes. Both the stimulation of intracellular CAT production and the inhibition of molecular CAT activity contributed to intracellular CAT activity change. Molecular mechanisms underlying CAT activity inhibition and structural changes were explored by isothermal titration calorimetry, multispectroscopy, and molecular docking studies. SDBS binds to CAT with 8.81 ± 0.751 sites via electrostatic forces, resulting in structural changes with α-helix significantly decreasing to 9.7 ± 1.2%. SDBS could interact with HIS 74, ASN 147, and TYR 357 around the active sites as well as TRP 185, ASP 127, and GLN 167 within the substrate channel and therefore might result in the inhibition of molecular CAT activity.
School of Environmental and Material Engineering, Yantai University , 30 Qingquan Road, Yantai 264005, People's Republic of China.
Jing
http://orcid.org/0000-0002-6142-7783
J
Wang
School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health , 27 Shanda South Road, Jinan, Shandong Province 250100, People's Republic of China.
Jiaxi
J
Wang
School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health , 27 Shanda South Road, Jinan, Shandong Province 250100, People's Republic of China.
Lu
L
Zhang
School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health , 27 Shanda South Road, Jinan, Shandong Province 250100, People's Republic of China.
Rutao
R
Liu
College of Population, Resources and Environment, Shandong Normal University , 88 East Wenhua Road, Jinan 250014, People's Republic of China.
Wansong
W
Zong
catalase; molecular mechanism; oxidative stress; sodium dodecylbenzenesulfonate; toxicity evaluation