The Effect of Butin on the Vitiligo Mouse Model Induced by Hydroquinone.
Vernonia anthelmintica (L.) Willd has been traditionally used in the treatment of vitiligo in Uyghur medicine. This study used butin, the main component of V. anthelmintica, to study the influence on hydroquinone-induced vitiligo in mice. The animals were randomly divided into six groups: control, model, 8-methoxypsoralen (8-MOP, 4.25 mg/kg), and butin (0.425, 4.25, and 42.5 mg/kg) groups. The number of melanin-containing hair follicles, basal layer melanocytes, melanin-containing epidermal cells, the expression of tyrosinase (TYR) and tyrosinase-related protein-1 (TRP-1), the malondialdehyde (MDA), and cholinesterase (CHE) activity in serum were measured. Our results indicated that compared with the model group, the melanin-containing hair follicles, the expression of TYR and TRP-1 increased, the activity of CHE decreased after treatment with 8-MOP and all doses of butin (p < 0.05, p < 0.01), the basal layer melanocytes and melanin-containing epidermal cells increased significantly after treatment with butin 4.25 and 42.5 mg/kg (p < 0.05, p < 0.01), and the MDA activity decreased after using butin 4.25 and 42.5 mg/kg and 8-MOP (p < 0.05, p < 0.01). Our results support the use of butin on vitiligo, and its possible mechanisms may be related to increase the TYR and TRP-1 protein expression and decrease the activity of MDA and CHE in hydroquinone-induced vitiligo model in mice. Copyright © 2017 John Wiley & Sons, Ltd.
Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uyghur Medicine, Urumqi, 830049, China.
Shi-Xia
SX
Huo
Preclinical Medicine Research Center/School of Pharmacy, Southwest Medical University, Luzhou, 646000, China.|Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, 100193, China.
Qiong
http://orcid.org/0000-0002-5323-5829
Q
Wang
Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uyghur Medicine, Urumqi, 830049, China.|Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, 100193, China.
Xin-Min
http://orcid.org/0000-0001-6452-4645
XM
Liu
Institute of Soil and Fertilizer & Agricultural Water Conservation, Xinjiang Academy of Agricultural Science, Urumqi, 830011, China.
Chun-Hui
CH
Ge
Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uyghur Medicine, Urumqi, 830049, China.
Li
L
Gao
Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uyghur Medicine, Urumqi, 830049, China.
Xiao-Ming
XM
Peng
Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uyghur Medicine, Urumqi, 830049, China.
Ming
M
Yan
D000818:Animals; D001578:Benzopyrans / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D004817:Epidermis; D018859:Hair Follicle / Q000378:metabolism; D006801:Humans; D006873:Hydroquinones / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde; D051379:Mice; D008517:Phytotherapy; D011897:Random Allocation; D035741:Vernonia / Q000737:chemistry; D014820:Vitiligo / Q000139:chemically induced* / Q000188:drug therapy*
D001578:Benzopyrans; D006873:Hydroquinones; C051437:butin; D008315:Malondialdehyde; C031927:hydroquinone
butin; hydroquinone; mouse; vitiligo