Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana.
The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract ofPhilenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests thatAllophylus spicatus,Philenoptera cyanescens,Melanthera scandens,Ocimum gratissimum, andJasminum dichotomumhave wound healing activity in vitro.
Department of Drug Design and Pharmacology, Universitetsparken 2, 2100 Copenhagen, Denmark.
Sara H
SH
Freiesleben
Department of Drug Design and Pharmacology, Universitetsparken 2, 2100 Copenhagen, Denmark; Museum of Natural Medicine, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark.
Jens
J
Soelberg
Department of Drug Design and Pharmacology, Universitetsparken 2, 2100 Copenhagen, Denmark.
Nils T
NT
Nyberg
Department of Drug Design and Pharmacology, Universitetsparken 2, 2100 Copenhagen, Denmark.
Anna K
0000-0002-3644-8208
AK
Jäger