Tubulointerstitial nephritis antigen-like 1 protein is downregulated in the placenta of pre-eclamptic women.
BACKGROUND
Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction. Research related to TINAGL1 is limited to cell culture and animal models. Demonstration of TINAGL1 as a positive regulator of angiogenesis and its expression in the decidua of postimplantation mouse uterus, prompted us to validate its expression in human placenta during impaired angiogenesis in pre-eclamptic condition.


METHODS
Placental tissue from normotensive (n = 25) and pre-eclamptic (n = 25) pregnancies were used to study the differentially expressed proteins by two-dimensional gel electrophoresis and TINAGL1 protein was validated with Western blotting.


RESULTS
A total of 55 protein spots were differentially expressed (fold change >1.5, p < 0.05), of which 27 were upregulated and 28 were downregulated in the pre-eclamptic placenta. TINAGL1 was found to be downregulated in pre-eclamptic compared to normotensive pregnant women.


CONCLUSIONS
This is the first study reporting TINAGL1 to be present in human placenta and differentially expressed in pre-eclamptic condition. The functional role of TINAGL1 in association to human pregnancy needs to be explored further.
Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008 India.
Sheon
S
Mary
Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008 India.
Mahesh J
MJ
Kulkarni
Department of Gynecology, Bharati Vidyapeeth Medical College, Pune, Maharashtra 411043 India.
Savita S
SS
Mehendale
Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, Maharashtra 411043 India.
Sadhana R
SR
Joshi
Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008 India.
Ashok P
AP
Giri
Human placenta; Matricellular protein; Pre-eclampsia; Proteomics; Tubulointerstitial nephritis antigen-like 1 protein