MicroRNA-206 regulates vascular smooth muscle cell phenotypic switch and vascular neointimal formation.
MiR-206 has been found to play a critical role in skeletal muscle proliferation, differentiation, and regeneration. However, little is known about the function of miR-206 in vascular smooth muscle cells (VSMCs) biology. In this study, we will investigate its roles in phenotypic switching of VSMCs and neointimal lesion formation. First, we identified the expression of miR-206 in VSMCs treated with various concentrations of TGFβ1 and in rat carotid arteries after angioplasty by using qPCR. TGFβ1 inhibited the expression of miR-206 and TGFβ1 inhibitor induced miR-206 expression. In VSMCs of injured vascular walls, miR-206 expression was upregulated. Then, we overexpressed miR-206 using lentivirus Lv-rno-mir-206 and knocked down miR-206 using LV-rno-mir-206-inhibitor in rat carotid arteries after angioplasty. Overexpression of miR-206 resulted in decreasing SM22α expression in VSMCs in vitro and knockdown of miR-206 suppressed neointimal lesion formation in vivo. Finally, ZFP580 (zinc finger protein 580) was identified as the direct target of miR-206 in VSMCs by using luciferase report assay. The results indicate that miR-206 is involved in phenotypic switching of VSMCs and neointimal lesion formation after angioplasty through targeting ZFP580. These findings may provide a novel therapeutic target in post-angioplasty restenosis.
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Huizhihuan Road 1, Dongli District, Tianjin, 300309, China.
Huiyan
H
Sun
Department of Cardiology, Affiliated Hospital, Logistics University of Chinese People's Armed Police Force, Chenglin Road 220, Dongli District, Tianjin, 300162, China.
Songzhi
S
Cai
Department of Cardiology, Affiliated Hospital, Logistics University of Chinese People's Armed Police Force, Chenglin Road 220, Dongli District, Tianjin, 300162, China.
Mei
M
Zhang
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Huizhihuan Road 1, Dongli District, Tianjin, 300309, China.
Juan
J
Zhao
Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Chenglin Road 220, Dongli District, Tianjin, 300162, China.
Shuping
S
Wei
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Huizhihuan Road 1, Dongli District, Tianjin, 300309, China.
Yuyu
Y
Luo
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Huizhihuan Road 1, Dongli District, Tianjin, 300309, China.
Xiangyan
X
Meng
Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Chenglin Road 220, Dongli District, Tianjin, 300162, China.
Xin
X
Zhou
Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Chenglin Road 220, Dongli District, Tianjin, 300162, China.
Yuming
Y
Li
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Huizhihuan Road 1, Dongli District, Tianjin, 300309, China.|Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Chenglin Road 220, Dongli District, Tianjin, 300162, China.
Wencheng
W
Zhang
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D035683:MicroRNAs; D053773:Transforming Growth Factor beta1; C522220:mirn206 microRNA, rat
ZFP580; miR-206; phenotypic switch; restenosis; vascular smooth muscle cells