Piper betle leaf extract affects the quorum sensing and hence virulence of Pseudomonas aeruginosa PAO1.
Quorum sensing (QS) plays an important role in virulence of Pseudomonas aeruginosa, blocking of QS ability are viewed as viable antimicrobial chemotherapy and which may prove to be a safe anti-virulent drug. Bioactive components from Piper betle have been reported to possess antimicrobial ability. This study envisages on the anti-QS properties of ethanolic extract of P. betle leaf (PbLE) using P. aeruginosa PAO1 as a model organism. A marked reduction in swarming, swimming, and twitching ability of the bacteria is demonstrated in presence of PbLE. The biofilm and pyocyanin production also shows a marked reduction in presence of PbLE, though it does not affect the bacterial growth. Thus, the studies hint on the possible effect of the bioactive components of PbLE on reducing the virulent ability of the bacteria; identification of bioactive compounds should be investigated further.
Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India. dattasiraj@gmail.com.
Siraj
S
Datta
Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India.
Debanjan
D
Jana
Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India.
Tilak Raj
TR
Maity
Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India.
Aveek
A
Samanta
Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India.
Rajarshi
R
Banerjee
Anti Quorum Sensing properties; Biofilm production; Mobility pattern; Piper betle leaf extract; Pseudomonas aeruginosa; Pyocyanin assay