Binding affinity and adhesion force of organophosphate hydrolase enzyme with soil particles related to the isoelectric point of the enzyme.
The binding affinity of organophosphate hydrolase enzyme (OphB) with soil particles in relation to the isoelectric point (pI) was studied. Immobilization of OphB with soil particles was observed by confocal microscopy, Fourier transform infrared spectroscopy (FT-IR), and Atomic force microscopy (AFM). The calculated pI of OphB enzyme was increased from 8.69 to 8.89, 9.04 and 9.16 by the single, double and triple mutant of OphB enzyme, respectively through the replacement of negatively charged aspartate with positively charged histidine. Practically, the binding affinity was increased to 5.30%, 11.50%, and 16.80% for single, double and triple mutants, respectively. In contrast, enzyme activity of OphB did not change by the mutation of the enzyme. On the other hand, adhesion forces were gradually increased for wild type OphB enzyme (90 pN) to 96, 100 and 104 pN for single, double and triple mutants of OphB enzyme, respectively. There was an increasing trend of binding affinity and adhesion force by the increase of isoelectric point (pI) of OphB enzyme.
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali 8602, Bangladesh. Electronic address: litonpath@yahoo.com.
Shah Md Asraful
SMA
Islam
Department of Forest Products, IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Shabina
S
Yeasmin
Department of Soil Science, Patuakhali Science and Technology University, Dumki, Patuakhali 8602, Bangladesh.
Md Saiful
MS
Islam
Department of Agricultural Chemistry, Patuakhali Science and Technology University, Dumki, Patuakhali 8602, Bangladesh.
Md Shariful
MS
Islam
D052918:Environmental Restoration and Remediation; D049452:Green Fluorescent Proteins / Q000235:genetics; D006639:Histidine / Q000235:genetics; D007526:Isoelectric Point; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010755:Organophosphates / Q000737:chemistry*; D010573:Pesticide Residues / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity
D010755:Organophosphates; D010573:Pesticide Residues; D012987:Soil; D012989:Soil Pollutants; D049452:Green Fluorescent Proteins; D006639:Histidine; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases
Adhesion force; Mutation; Organophosphate hydrolase enzyme; Organophosphate pesticide; Soil binding