Green Synthesis of Hierarchically Structured Silver-Polymer Nanocomposites with Antibacterial Activity.
The in situ formation of silver nanoparticles (AgNPs) aided by chondroitin sulfate and the preparation of a hierarchically structured silver-polymer nanocomposite with antimicrobial activity is shown. Green synthesis of AgNPs is carried out by thermal treatment (80 and 90 °C) or UV irradiation of a chondroitin sulfate solution containing AgNO₃ without using any further reducing agents or stabilizers. Best control of the AgNPs size and polydispersity was achieved by UV irradiation. The ice-segregation-induced self-assembly (ISISA) process, in which the polymer solution containing the AgNPs is frozen unidirectionally, and successively freeze-drying were employed to produce the chondroitin sulfate 3D scaffolds. The scaffolds were further crosslinked with hexamethylene diisocyanate vapors to avoid water solubility of the 3D structures in aqueous environments. The antimicrobial activity of the scaffolds was tested againstEscherichia coli. The minimum inhibitory concentration (MIC) found for AgNPs-CS (chondroitin sulfate) scaffolds was ca. 6 ppm.
Instituto de Ciencia de los Materiales, Consejo Superior de Investigaciones Científicas, Campus of Cantoblanco, Madrid 28049, Spain. mjhortiguela@gmail.com.
María Jesús
MJ
Hortigüela
Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus of Cantoblanco, Madrid 28049, Spain. lyuste@cnb.csic.es.
Luis
L
Yuste
Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus of Cantoblanco, Madrid 28049, Spain. frojo@cnb.csic.es.
Fernando
F
Rojo
Instituto de Ciencia de los Materiales, Consejo Superior de Investigaciones Científicas, Campus of Cantoblanco, Madrid 28049, Spain. iaranaz@hotmail.com.
Inmaculada
I
Aranaz
chondroitin sulfate; nanoparticles; scaffolds; silver