Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production.
Various chemicals, i.e., furfural, vanillin, 4-hydroxybenzaldehyde and acetate produced during the pretreatment of biomass affect microbial fermentation. In this study, effect of vanillin, 4-hydroxybenzaldehyde and acetate on antibiotic production in Streptomyces coelicolor is investigated. IC50value of vanillin, 4-hydroxybenzaldehyde and acetate was recorded as 5, 11.3 and 115 mM, respectively. Vanillin was found as a very effective molecule, and it completely abolished antibiotic (undecylprodigiosin and actinorhodin) production at 1 mM concentration, while 4-hydroxybenzaldehyde and acetate have little effect. Microscopic analysis with field emission scanning electron microscopy (FESEM) showed that addition of vanillin inhibits mycelia formation and increases differentiation of S. coelicolor cells. Vanillin increases expression of genes responsible for sporulation (ssgA) and decreases expression of antibiotic transcriptional regulator (redD and actII-orf4), while it has no effect on genes related to the mycelia formation (bldA and bldN) and quorum sensing (scbA and scbR). Vanillin does not affect the glycolysis process, but may affect acetate and pyruvate accumulation which leads to increase in fatty acid accumulation. The production of antibiotics using biomass hydrolysates can be quite complex due to the presence of exogenous chemicals such as furfural and vanillin, and needs further detailed study.
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.|Institute for Ubiquitous Information Technology and Applications Konkuk University, Seoul, 143-701, South Korea.
Shashi Kant
SK
Bhatia
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.
Bo-Rahm
BR
Lee
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.
Ganesan
G
Sathiyanarayanan
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.
Hun Seok
HS
Song
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.
Junyoung
J
Kim
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.
Jong-Min
JM
Jeon
IT Convergence Materials R&BD Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 35-3 Hongchon-ri, Ipjang-myun, Seobuk-gu, Chonan-si, Chungnam, 330-825, South Korea.
Jeong-Jun
JJ
Yoon
Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-Gu, Daejeon, 305-806, South Korea.
Jungoh
J
Ahn
Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong, South Korea.
Kyungmoon
K
Park
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea. seokor@konkuk.ac.kr.|Institute for Ubiquitous Information Technology and Applications Konkuk University, Seoul, 143-701, South Korea. seokor@konkuk.ac.kr.
Yung-Hun
YH
Yang
Antibiotic; Biomass; Furfural; Streptomyces coelicolor; Vanillin