Expression analysis of Toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure.
Six male Tharparkar cattle of 2-3 years old were selected for the study. After 15 days acclimation at thermo neutral zone (TNZ) in psychrometric chamber, animals were exposed at 42°C for 6h up to 23 days followed by 12 days of recovery period. Blood samples were collected during control period at TNZ (day 1, 5 and 12), after heat stress exposure (day 1-10, Short Term Heat Stress Acclimation - STHSA; day 15-23, Long Term Heat Stress Acclimation - LTHSA) and recovery period (day 7 and 12) and peripheral blood mononuclear cells (PBMCs) were isolated for RNA and protein extraction. Serum cortisol concentration was assessed by RIA. The mRNA and protein expression in PBMCs were determined by qPCR and western blot respectively. Samples at TNZ were taken as control. Serum cortisol concentration was increased (P<0.05) during STHSA and gradually declined during LTHSA. Toll like receptor 2 (TLR 2) expression was up regulated (P<0.05) during STHSA and declined to basal level during LTHSA and recovery phase. However, toll like receptor 4 (TLR 4) expression was up regulated (P<0.05) during STHSA and LTHSA while declined in recovery phase. Interleukin 2 (IL2) and interleukin 6 (IL 6) were up regulated (P<0.05) during STHSA and reduced to basal level during LTHSA. PBMCs culture study was conducted to study transcriptional abundance of TLR2/4 and IL2/6 at different temperature-time combinations. The present findings indicate that TLR 2/4 and IL 2/6 could possibly play a vital role in thermo tolerance in Tharparkar cattle during short term and long term heat stress exposure.
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
Jaya
J
Bharati
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
S S
SS
Dangi
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
S R
SR
Mishra
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
V S
VS
Chouhan
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
V
V
Verma
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
O
O
Shankar
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
M K
MK
Bharti
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
A
A
Paul
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
Dilip K
DK
Mahato
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
G
G
Rajesh
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
G
G
Singh
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
V P
VP
Maurya
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
S
S
Bag
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
Puneet
P
Kumar
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India. Electronic address: msarkar24@gmail.com.
M
M
Sarkar
D000064:Acclimatization; D000818:Animals; D002417:Cattle / Q000097:blood / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D057232:Global Warming; D006358:Hot Temperature; D006854:Hydrocortisone / Q000097:blood; D007378:Interleukins / Q000235:genetics*; D008297:Male; D013312:Stress, Physiological; D051193:Toll-Like Receptors / Q000235:genetics*
D007378:Interleukins; D051193:Toll-Like Receptors; D006854:Hydrocortisone
Heat stress; Interleukin; PBMCs; Tharparkar cattle; Toll like receptor