Antibacterial properties of amino acid functionalized silver nanoparticles decorated on graphene oxide sheets.
Graphene oxide (GO) sheets decorated with amino acid L-cysteine (L-cys) functionalized silver nanoparticles (GO-L-cys-Ag) was synthesized by AgNO3, trisodium citrate, and NaBH4. GO-L-cys-Ag nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, which demonstrated that a diameter of L-cys-AgNPs compactly deposited on GO. Antibacterial activity tests of GO-L-cys-Ag nanocomposite were carried out using Escherichia coli MTCC 1687 and Staphylococcus aureus MTCC 3160 as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage on antibacterial activity of GO-L-cys-Ag nanocomposite was examined by plate count, well diffusion and broth dilution methods. Morphological observation of bacterial cells by scanning electron microscope (SEM) showed that GO-L-cys-Ag nanocomposite was more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. The above technique establish that the bactericidal property of GO-L-cys-Ag nanocomposite with wide range of applications in biomedical science.
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India.
Kumudini
K
Chandraker
Department of Chemistry, Govt. Madhav Science P. G. College, Ujjain 456010, (M.P.), India.
Rekha
R
Nagwanshi
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India.
S K
SK
Jadhav
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India.
Kallol K
KK
Ghosh
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India. Electronic address: manmohanchem@gmail.com.
Manmohan L
ML
Satnami
D000596:Amino Acids / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D006108:Graphite / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013057:Spectrum Analysis
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D010087:Oxides; D012834:Silver; D006108:Graphite
Antibacterial activity; GO-L-cys-Ag nanocomposite; Graphene oxide; L-cys-AgNPs; SEM