Possible protective effect of royal jelly against cyclophosphamide induced prostatic damage in male albino rats; a biochemical, histological and immuno-histo-chemical study.
Almost all the chemotherapy treat many cancer types effectively, but it leads to severe side effects. Chemotherapy like cyclophosphamide (CP) not works only on the active cells, such as cancer cells, but also acts on the healthy cells. Royal jelly (RJ) was reported to have a lot of therapeutic effects besides being an anti-oxidant and anti-cancer agent. The purpose of this study was to assess the possible protective role of RJ in ameliorating the toxic effects of CP overdose in the rat prostatic tissue. The rats were separated into 4 groups; control group, RJ group, CP group and RJ with CP group. Prostatic specimens were processed for biochemical, histological and immune-histo-chemical studies. The mean area fractions of eNOS and Bax expression were measured in all groups, and statistical analysis was carried out. The results showed that in CP treated group, there were marked biological changes in the form of significant increase in prostatic malondialdehyde (MDA) and C - reactive protein (CRP). Additionally there was a significant decrease in glutathione peroxidase (GPx) in prostatic tissue if compared with the control group. Furthermore, the histological changes showed marked acinar and stromal prostatic degeneration. Most prostatic acini showed less PAS reaction and more (eNOS and Bax) expression if compared with the control group. Concomitant administration of RJ with CP revealed a noticeable amelioration of these biochemical and histological changes. In conclusion, RJ provided biochemical and histo-pathological improvement in CP induced prostatic tissue toxicity. These findings revealed that this improvement was associated with a decrease in the tissue oxidative damage and apoptosis.
Department of Histology, Faculty of Medicine, Minia University, Egypt. Electronic address: sara_histology@yahoo.com.
Sara Mohammed Naguib
SMN
Abdel-Hafez
Department of Histology, Faculty of Medicine, Minia University, Egypt. Electronic address: rehabrifaai@yahoo.com.
Rehab Ahmed
RA
Rifaai
Department of Pharmacology, Faculty of Medicine, Minia University, Egypt. Electronic address: walaayehia22@yahoo.com.
Walaa Yehia
WY
Abdelzaher
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002097:C-Reactive Protein / Q000378:metabolism; D003520:Cyclophosphamide / Q000009:adverse effects*; D005227:Fatty Acids / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D000099219:Royal Jelly
D000975:Antioxidants; D002097:C-Reactive Protein; D003520:Cyclophosphamide; D005227:Fatty Acids; D005978:Glutathione; D005979:Glutathione Peroxidase; D008315:Malondialdehyde; D052250:Nitric Oxide Synthase Type III; D020011:Protective Agents; D051028:bcl-2-Associated X Protein; D000099219:Royal Jelly
Cyclophosphamide; Prostatic tissue; Royal jelly