Diabetes consequences in the fetus liver of the non-obese diabetic mice.
DM type 1 (T1D) incidence is increasing around 3% every year and represents risks for maternal and fetal health. The objective of this study was to explore the effects of diabetes on fetus liver cells in non-obese diabetic (NOD) mice. Hyperglycemic NOD (HNOD), normoglycemic NOD (NNOD) and BALB/c females were used for mating, and the fetus livers were collected at 19.5 gestation day (gd). HNOD group had reduced fetal weight (989.5±68.32 vs 1290±57.39 mg BALB/c, P<0.05) at 19.5 gd and higher glycemia (516.66±28.86 mg dl-1, P<0.001) at both 0.5 gd and 19.5 gd compared to other groups. The protein expression of albumin (ALB) was significantly reduced in HNOD group (0.9±0.2 vs 3.36±0.36 NNOD P<0.01, vs 14.1±0.49 BALB/c P<0.001). Reduced gene expression of ALB (1.34±0.12 vs 5.53±0.89 NNOD and 5.23±0.71 BALB/c, P<0.05), Hepatic Nuclear Factor-4 alpha (HNF-4α) (0.69±0.1 vs 3.66±0.36 NNOD, P<0.05) and miR-122 (0.27±0,10 vs 0.88±0.15 NNOD, P<0.05) was present in HNOD group. No difference for alpha-Fetoprotein (AFP) and gene expression was observed. In conclusion, our findings show the impacts of T1D on the expression of ALB, AFP, HNF-4α and miR-122 in fetus liver cells by using NNOD and HNOD mice.
Department of Morphology, Federal University of Sergipe, Sao Cristovao, Sergipe, Brazil.
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MB
Aires
Department of Morphology, Federal University of Sergipe, Sao Cristovao, Sergipe, Brazil.
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Dos Santos
Discipline of Liver Transplant and Digestive Organs (LIM-37), Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
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MS
Kubrusly
Laboratory of Biochemistry and Biophysics, Butantan Institute, Sao Paulo, Brazil.
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de Lima Luna
Discipline of Liver Transplant and Digestive Organs (LIM-37), Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
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D'Albuquerque
Laboratory of Biochemistry and Biophysics, Butantan Institute, Sao Paulo, Brazil.
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Maria
D000418:Albumins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005333:Fetus; D015870:Gene Expression; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D016688:Mice, Inbred NOD; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism
D000418:Albumins; D051557:Hepatocyte Nuclear Factor 4; C496593:Hnf4a protein, mouse; D035683:MicroRNAs; C544354:Mirn122 microRNA, mouse