Properties of An Oral Nanoformulation of A Molecularly Dispersed Amphotericin B Comprising A Composite Matrix of Theobroma Oil and Bee'S Wax.
An amphotericin B-containing (AmB) solid lipid nanoparticulate drug delivery system intended for oral administration, comprised of bee's wax and theobroma oil as lipid components was formulated with the aim to ascertain the location of AmB within the lipid matrix: (a) a homogenous matrix; (b) a drug-enriched shell; or (c) a drug enriched core. Both the drug-loaded and drug-free nanoparticles were spherical with AmB contributing to an increase in both thez-average diameter (169 ± 1 to 222 ± 2 nm) and zeta potential (40.8 ± 0.9 to 50.3 ± 1.0 mV) of the nanoparticles. A maximum encapsulation efficiency of 21.4% ± 3.0%, corresponding to 10.7 ± 0.4 mg encapsulated AmB within the lipid matrix was observed. Surface analysis and electron microscopic imaging indicated that AmB was dispersed uniformly within the lipid matrix (option (a) above) and, therefore, this is the most suitable of the three models with regard to modeling the propensity for uptake by epithelia and release of AmB in lymph.
School of Pharmacy, The University of Nottingham, Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia. tanseewei@hotmail.co.uk.
Chloe See Wei
CS
Tan
School of Pharmacy, The University of Nottingham, Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia. nashiru.billa@nottingham.edu.my.
Nashiru
N
Billa
School of Pharmacy, The University of Nottingham, Park Campus, Nottingham NG7 2RD, UK. clive.roberts@nottingham.ac.uk.
Clive J
CJ
Roberts
School of Pharmacy, The University of Nottingham, Park Campus, Nottingham NG7 2RD, UK. david.scurr@nottingham.ac.uk.
David J
DJ
Scurr
amphotericin B (AmB); beeswax; cellular uptake; lipid; nanoparticles; theobroma oil