Biostimulation with diode laser positively regulates cementoblast functions, in vitro.
The aim of this study was to evaluate the effects of diode laser biostimulation on cementoblasts (OCCM.30). A total of 40 root plates were obtained from healthy third molar teeth and assigned to the following two groups: (1) control group and (2) laser-treated group. Root plates were placed into the cell culture inserts, and OCCM.30 cells were seeded onto root plates. Cells were irradiated with a low level of diode laser (power: 0.3 W in continuous wave, 60 s/cm2). Proliferation and mineralized tissue-associated gene's and BMP's messenger RNA (mRNA) expressions of cementoblasts were evaluated. Total RNAs were isolated on day 3 and integrin-binding sialoprotein (Ibsp), bone gamma-carboxyglutamate protein (Bglap), Type I collagen (Col1a1), osteoblastic transcription factor, runt-related transcription factor (Runx2), and Bone Morphogenetic Protein (BMP)-2, 3, 4, 6, and 7 mRNA expressions were determined using quantitative RT-PCR. von Kossa staining was performed to evaluate biomineralization of OCCM.30 cells. In the proliferation experiment, while there was no significant difference until 96 h, laser irradiation retarded the decrease in cell proliferation trend after 96 h compared to the untreated control group. Statistically significant increase in Ibsp, Bglap, and BMP-2,3,6,7 mRNA expressions were noted in the laser groups when compared to the untreated control group (p < 0.05). Laser irradiation induced mineralized nodule formation of cementoblasts. The results of this study reveal that the biostimulation setting of diode laser modulates the behavior of cementoblasts inducing mineralized tissue-associated gene's mRNA expressions and mineralization. Therefore, biostimulation can be used during regenerative periodontal therapies to trigger cells with periodontal attachment apparatus.
Faculty of Dentistry, Research Center, Selcuk University, Konya, Turkey.
Serife Buket
SB
Bozkurt
Faculty of Agriculture, Department of Soil Science and Plant Nutrition, Molecular Genetics & Biotechnology Laboratories, Selcuk University, Konya, Turkey.
Erdogan E
EE
Hakki
Faculty of Medicine, Department of Biostatistics, Karabuk University, Karabuk, Turkey.
Seyit Ali
SA
Kayis
Faculty of Dentistry, Research Center, Selcuk University, Konya, Turkey.
Niyazi
N
Dundar
Faculty of Dentistry, Research Center, Selcuk University, Konya, Turkey. sshakki@yahoo.com.|Faculty of Dentistry, Department of Periodontology, Selcuk University, 42079, Konya, Turkey. sshakki@yahoo.com.
Sema S
SS
Hakki
D000818:Animals; D002113:Calcification, Physiologic / Q000235:genetics / Q000528:radiation effects; D002448:Cell Adhesion / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D003739:Dental Cementum / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy; D051379:Mice; D008963:Molar / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014092:Tooth Root / Q000737:chemistry / Q000528:radiation effects
D012333:RNA, Messenger
Biostimulation; Cementum; Gene expression; Laser; Mineralization; Regeneration