Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity.
A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.
School of Food Sciences and Engineering, South China University of Technology, Wushan road 381, Tianhe District, 510640 Guangzhou, P.R. China.
Xu-Ping
XP
Wang
School of Bioscience and Bioengineering, South China University of Technology, Daxuecheng, Panyu District, 510006 Guangzhou, P.R. China.
Peng-Fei
PF
Zhou
School of Bioscience and Bioengineering, South China University of Technology, Daxuecheng, Panyu District, 510006 Guangzhou, P.R. China.
Zhi-Gang
ZG
Li
School of Bioscience and Bioengineering, South China University of Technology, Daxuecheng, Panyu District, 510006 Guangzhou, P.R. China.
Bo
B
Yang
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands.
Frank
F
Hollmann
School of Food Sciences and Engineering, South China University of Technology, Wushan road 381, Tianhe District, 510640 Guangzhou, P.R. China.
Yong-Hua
YH
Wang
D055162:Biocatalysis; D002175:Candida / Q000201:enzymology*; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D010084:Oxidation-Reduction; D015202:Protein Engineering / Q000379:methods*; D012997:Solvents; D013379:Substrate Specificity
D012997:Solvents; D006867:Hydrolases; D008049:Lipase