Effect ofPunica granatumfruit peel on glucose-6-phosphate dehydrogenase and malate dehydrogenase in amphistomeGastrothylax indicus.
Increasing anthelmintic resistance and the impact of conventional anthelmintics on the environment, it is important to look for alternative strategies against helminth parasite in sheep. Important lipogenic enzymes like glucose-6-phosphate dehydrogenase (G-6-PDH) and malate dehydrogenase (MDH) show subcellular distribution pattern. Activity of G-6-PDH was largely restricted to cytosolic fraction while MDH was found in both cytosolic and mitochondrial fraction inGastrothylax indicus.Following in vitro treatment with ethanolic and aqueous extracts ofPunica granatumfruit peel and commercial anthelmintic, albendazole G-6-PDH activity was decreased by 19-32 %, whereas MDH was suppressed by 24-41 %, compared to the respective control. Albendazole was quite effective when compared with negative control and both the extracts. The results indicate that phytochemicals of plant may act as potential vermifuge or vermicide.
Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014 India.
Rama
R
Aggarwal
Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014 India.
Upma
U
Bagai
Anthelmintic; Gastrothylax indicus; Glucose-6-phosphate dehydrogenase; Malate dehydrogenase; Punica granatum