The Influences of Bacillus subtilis on the Virulence of Aeromonas hydrophila and Expression of luxS Gene of Both Bacteria Under Co-cultivation.
The aim of this study was to explore the influence of Bacillus subtilis CH9 on Aeromonas hydrophila SC2005. The transcription level of virulence genes of A. hydrophila SC2005 and its hemolysin activity as well as its cytotoxicity were analyzed when B. subtilis CH9 and A. hydrophila SC2005 were co-cultured. The results indicated that the transcription levels of four virulence genes of A. hydrophila, including aer, ahyB, hcp, and emp, decreased when A. hydrophila was cultured with B. subtilis CH9. Furthermore, the extracellular products of A. hydrophila showed attenuated hemolysin activity as well as cytotoxicity when A. hydrophila was cultured with B. subtilis CH9. Finally, the transcriptional levels of luxS genes of B. subtilis CH9 and A. hydrophila SC2005 were determined when these two species were co-cultured. RT-qPCR results suggested that the transcription level of A. hydrophila was down-regulated significantly. On the contrary, the transcription level of B. subtilis CH9 was up-regulated significantly. These results suggested that the probiotic role of B. subtilis CH9 is related to the inhibition of growth and virulence of A. hydrophila SC2005, and quorum sensing may be involved.
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China.
Yuwei
Y
Ren
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China.
Sisi
S
Li
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China.
Zhixin
Z
Wu
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China.
Chengchong
C
Zhou
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China.
Ding
D
Zhang
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. chenxx@mail.hzau.edu.cn.|Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China. chenxx@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China. chenxx@mail.hzau.edu.cn.
Xiaoxuan
X
Chen
D016980:Aeromonas hydrophila / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism* / Q000472:pathogenicity; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D013437:Carbon-Sulfur Lyases / Q000096:biosynthesis*; D002352:Carrier Proteins / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D006460:Hemolysin Proteins / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis; D010196:Pancreatic Elastase / Q000096:biosynthesis; D019936:Probiotics / Q000378:metabolism*; D053038:Quorum Sensing / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D037521:Virulence Factors / Q000096:biosynthesis* / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D006460:Hemolysin Proteins; D007506:Iron-Sulfur Proteins; D037521:Virulence Factors; C409565:ahyB protein, Aeromonas hydrophila; D010196:Pancreatic Elastase; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria
Aeromonas hydrophila; Bacillus subtilis; Co-cultivation; LuxS gene; Virulence