Simultaneous determination of eight alkaloids and oleandrin in herbal cosmetics by dispersive solid-phase extraction coupled with ultra high performance liquid chromatography and tandem mass spectrometry.
We utilized ultra-high performance liquid chromatography with tandem mass spectrometry and dispersive solid-phase extraction to develop a new method for the detection of nine analytes (scopolamine, cephaeline, strychnine, hyoscyamine, brucine, hydrastine, ajmalicine, colchicine, and oleandrin) in herbal cosmetics. Acetonitrile/water and 2-propylaminoethylamine were used to disperse and purify during the dispersive solid-phase extraction step. The analytes were separated by a Waters UPLC HSS T3 column and detected through electrospray ionization source in the positive mode with multi-reaction monitoring conditions. Under the optimal conditions, the calibration curves were linear in the range of 0.2-100.0 μg/L with the correlation coefficients higher than 0.995. The method limit of quantitation (S/N = 10) were 5.0 μg/kg for oleandrin and 1.0 μg/kg for the other eight alkaloids. The mean recoveries at three spiked concentration levels of 1.0-10.0 μg/kg were in the range of 86.9-116.5% with the intra-day relative standard deviations (n = 6) ranging from 2.4 to 8.8%, and inter-day relative standard deviations ranging from 2.7 to 5.7%. This method is accurate, simple and rapid, and has been applied to the quality supervision of herbal cosmetics in Guangzhou.
National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Quality Supervision and Testing Institute, Guangzhou, China.
Zhiqing
Z
Xun
National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Quality Supervision and Testing Institute, Guangzhou, China.
Donghong
D
Liu
National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Quality Supervision and Testing Institute, Guangzhou, China.
Rongrong
R
Huang
National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Quality Supervision and Testing Institute, Guangzhou, China.
Shuang
S
He
National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Quality Supervision and Testing Institute, Guangzhou, China.
Du
D
Hu
National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Quality Supervision and Testing Institute, Guangzhou, China.
Xindong
X
Guo
National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou Quality Supervision and Testing Institute, Guangzhou, China.
Yanping
Y
Xian
D000470:Alkaloids / Q000032:analysis*; D002298:Cardenolides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
D000470:Alkaloids; D002298:Cardenolides; D003358:Cosmetics; C021065:oleandrin
alkaloids; herbal cosmetics; liquid chromatography; mass spectrometry; solid-phase extraction