Expression of a MORN repeat protein from Euplotes octocarinatus requires a +1 programmed ribosomal frameshifting.
Analysis of transcriptome revealed that a membrane occupation and recognition nexus (MORN) repeat protein-encoding gene of Euplotes octocarinatus (Eo-morn-9-31) was a candidate for programmed +1 ribosomal frameshifting (+1 PRF). In this study, a dual-luciferase assay was performed to detect its expression. The result showed that the MORN repeat protein (Eo-MORN-9-31) could be produced by the +1 PRF event during the process of translation in yeast and the frameshifting efficiency was about 4-5%. We further confirmed its reality by western blot and mass spectrometry. This study provided experimental evidence indicating that the expression of the Eo-MORN-9-31 of E. octocarinatus required the +1 PRF.
a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Lili
L
Wei
a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Xuemei
X
Zhao
a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Ruanlin
R
Wang
a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Yuejun
Y
Fu
a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Baofeng
B
Chai
a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Aihua
A
Liang
D001483:Base Sequence; D001681:Biological Assay; D003001:Cloning, Molecular; D016812:Euplotes / Q000235:genetics* / Q000378:metabolism; D018965:Frameshifting, Ribosomal; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome
D009687:Nuclear Proteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D008156:Luciferases
Euplotes octocarinatus; MORN; dual-luciferase experiment; mass spectrometry; programmed ribosomal frameshifting