[Exploratory study on influence of biopsy to biological behavior of Tca8113 transplanted tumor].
OBJECTIVE
We established an animal model of nude mice with Tca8113 tumor and cut some tissue for biopsy. We also determined the biological behavior and mechanisms of the tumor.


METHODS
The mice were divided into two groups randomly. Mice in both groups were injected with Tca8113 cells into their tongues. The survival condition, growth of primary focus, and metastasis were observed. Hematoxylin and eosin staining and immunohistochemistry were performed on nuclear factor κB (NF-κB), matrix metallopeptidase 9 (MMP-9), vascular endothelial growth factor (VEGF), stromal cell-derived factor 1 (SDF-1), and Ki67 to determine their distributions within the tumor. Cytokeratin staining was also performed to detect micrometastasis in the submandibular lymph nodes.


RESULTS
The emerging rate of tumor was 97.92%. The weight and survival time of the experimental group were lower than that of the control group, whereas the metastasis ratio was higher. The expression of NF-κB, MMP-9, SDF-1, and MMP-9 in tumors was higher in the experimental group than that in the control group. The expression of NF-κB, MMP-9, VEGF, and SDF-1 was relevant. The microvessel density of the experimental group was higher than that in the control group.


CONCLUSIONS
Biopsy can affect the biological behavior of tongue tumor and can promote growth of primary focus and metastasis.
State Key Laboratory of Oral Diseases, Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Liu
L
Jiyuan
State Key Laboratory of Oral Diseases, Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Pan
P
Jian
State Key Laboratory of Oral Diseases, Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Hua
H
Chengge
State Key Laboratory of Oral Diseases, Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Wu
W
Yunlong
State Key Laboratory of Oral Diseases, Dept. of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Luo
L
Zhengwen
State Key Laboratory of Oral Diseases, Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Tang
T
Xiufa
D000818:Animals; D001706:Biopsy; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008198:Lymph Nodes; D051379:Mice; D008819:Mice, Nude; D016328:NF-kappa B; D061206:Neoplasm Micrometastasis; D009369:Neoplasms; D042461:Vascular Endothelial Growth Factor A
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D016328:NF-kappa B; D042461:Vascular Endothelial Growth Factor A
biopsy; tongue cancer; tumor growth; tumor metastasis