Polymerization-Induced Self-Assembly (PISA) - Control over the Morphology of19F-Containing Polymeric Nano-objects for Cell Uptake and Tracking.
Fluorine-containing polymeric materials are receiving increasing attention as imaging probes in fluorine-19 magnetic resonance imaging (19F MRI), for example to enable quantitative in vivo detection of cells. Here we describe the one-pot polymerization synthesis of19F-containing functional poly(oligo(ethylene glycol) methyl ether methacrylate-co-2,2,2-trifluoroethyl acrylate-b-poly(styrene-co-3-vinylbenzaldehyde) (poly(OEGA-co-TFEA)-b-poly(St-co-VBA)) copolymers as a new class of fluorinated MRI agent. A range of nanoparticle morphologies, including spheres, worm-like particles, and vesicles were formed as a consequence of polymerization-induced self-assembly (PISA). It was found that the extent of cell uptake strongly depends on the morphology of the nano-objects, with preferable uptake for worm-like particles compared to spherical nanoparticles and vesicles. All the nano-objects have a single resonance in the19F NMR spectrum with relatively short MRI relaxation times, which were independent of the morphology of the nano-objects. These results confirm that these polymeric nano-objects of varied morphologies are promising as19F MRI imaging agents for use in tracking of cells and selective MRI.
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, St. Lucia, Queensland 4072, Australia.|ARC Centre of Excellence in Convergent Bio-Nano Science and Technology , Brisbane, Queensland 4072, Australia.
Wei
W
Zhao
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, St. Lucia, Queensland 4072, Australia.|ARC Centre of Excellence in Convergent Bio-Nano Science and Technology , Brisbane, Queensland 4072, Australia.
Hang T
0000-0003-1188-0472
HT
Ta
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, St. Lucia, Queensland 4072, Australia.|ARC Centre of Excellence in Convergent Bio-Nano Science and Technology , Brisbane, Queensland 4072, Australia.
Cheng
C
Zhang
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, St. Lucia, Queensland 4072, Australia.|ARC Centre of Excellence in Convergent Bio-Nano Science and Technology , Brisbane, Queensland 4072, Australia.
Andrew K
0000-0002-1948-8355
AK
Whittaker
D000818:Animals; D016466:CHO Cells; D058948:Cell Tracking; D003287:Contrast Media / Q000737:chemistry*; D003412:Cricetulus; D005456:Fluorescent Dyes; D005461:Fluorine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics*
D003287:Contrast Media; D005456:Fluorescent Dyes; D011108:Polymers; D005461:Fluorine