HSV-1 ICP27 represses NF-κB activity by regulating Daxx sumoylation.
Herpes simplex virus type 1 ICP27 is a multifunctional protein responsible for viral replication, late gene expression, and reactivation from latency. ICP27 interacts with various cellular proteins, including Daxx. However, the role of interaction between ICP27 and Daxx is largely unknown. Since Daxx is known to repress NF-κB activity, there is a possibility that ICP27 may influence the inhibitory effect of Daxx on NF-κB activity. In this study, we tested whether ICP27 affects the NF-κB activity through its interaction with Daxx. Interestingly, ICP27 enhanced the Daxx-mediated repression of NF-κB activity. In addition, we found that sumoylation of Daxx regulates its interaction with p65. ICP27 binds to Daxx, inhibits Daxx sumoylation, and enhances p65 deacetylation induced by Daxx. Consequently, ICP27 represses the NF-B activity, by elevating the inhibitory effect of Daxx on NF-κB activity through desumoylation of Daxx. [BMB Reports 2017; 50(5): 275-280].
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Ji Ae
JA
Kim
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Mi Sun
MS
Choi
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Jung Sun
JS
Min
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Inho
I
Kang
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Jeongho
J
Oh
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Jin Chul
JC
Kim
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Jeong Keun
JK
Ahn
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism* / Q000502:physiology; D056970:Co-Repressor Proteins; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D057809:HEK293 Cells; D018259:Herpesvirus 1, Human; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism* / Q000502:physiology*; D018832:Molecular Chaperones; D016328:NF-kappa B / Q000378:metabolism / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction; D058207:Sumoylation / Q000502:physiology; D051996:Transcription Factor RelA / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects
D048868:Adaptor Proteins, Signal Transducing; D056970:Co-Repressor Proteins; C106985:DAXX protein, human; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D018832:Molecular Chaperones; D016328:NF-kappa B; D009687:Nuclear Proteins; D051996:Transcription Factor RelA; D014157:Transcription Factors; D014764:Viral Proteins