Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content.
Botryococcus braunii can produce both long-chain hydrocarbons as well as carbohydrates in large quantities, and is therefore a promising industrial organism for the production of biopolymer building blocks. Many studies describe the use of different strains of Botryococcus braunii but differences in handling and cultivation conditions make the comparison between strains difficult. In this study, 16 B. braunii strains obtained from six culture collections were compared for their biomass productivity and hydrocarbon and carbohydrate content. Biomass productivity was highest for AC768 strain with 1.8gL-1day-1, while hydrocarbon production ranged from none to up to 42% per gram biomass dry weight, with Showa showing the highest hydrocarbon content followed by AC761. The total carbohydrate content varied from 20% to 76% per gram of the biomass dry weight, with CCALA777 as the highest producer. Glucose and galactose are the main monosaccharides in most strains and fucose content reached 463mgL-1in CCALA778.
Wageningen University and Research, Bioprocess Engineering Group, AlgaePARC, Droevendaalsesteeg 1, 6708 PB, The Netherlands. Electronic address: joao.gouveia@wur.nl.
Joao Diogo
JD
Gouveia
Wageningen UR Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
Jesus
J
Ruiz
Wageningen UR Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
Lambertus A M
LAM
van den Broek
Plant Research International, Business Unit of Bioscience, Cluster Applied Bioinformatics, Plant Research International, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
Thamara
T
Hesselink
Plant Research International, Business Unit of Bioscience, Cluster Applied Bioinformatics, Plant Research International, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
Sander
S
Peters
Wageningen UR Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
Dorinde M M
DMM
Kleinegris
Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
Alison G
AG
Smith
Wageningen University and Research, Bioprocess Engineering Group, AlgaePARC, Droevendaalsesteeg 1, 6708 PB, The Netherlands.
Douwe
D
van der Veen
Wageningen UR Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
Maria J
MJ
Barbosa
Wageningen University and Research, Bioprocess Engineering Group, AlgaePARC, Droevendaalsesteeg 1, 6708 PB, The Netherlands; University of Nordland, Faculty of Biosciences and Aquaculture, N-8049 Bodø, Norway.
Rene H
RH
Wijffels
D061249:Batch Cell Culture Techniques; D018533:Biomass; D000460:Chlorophyta / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005643:Fucose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005690:Galactose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006838:Hydrocarbons / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D058433:Photobioreactors; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D004269:DNA, Bacterial; D006838:Hydrocarbons; D012337:RNA, Ribosomal, 18S; D005643:Fucose; D005690:Galactose
Botryococcus braunii; Carbohydrate; Fucose; Galactose; Hydrocarbons; Microalgae