Exogenous application of plant growth regulators (PGRs) induces chilling tolerance in short-duration hybrid maize.
Chilling stress hampers the optimal performance of maize under field conditions precipitously by inducing oxidative stress. To confer the damaging effects of chilling stress, the present study aimed to investigate the effects of some natural and synthetic plant growth regulators, i.e., salicylic acid (SA), thiourea (TU), sorghum water extract (SWE), and moringa leaf extract (MLE) on chilling stress tolerance in autumn maize hybrid. Foliar application of growth regulators at low concentrations was carried out at six leaf (V6) and tasseling stages. An increase in crop growth rate (CGR), leaf area index (LAI), leaf area duration (LAD), plant height (PH), grain yield (GY), and total dry matter accumulation (TDM) was observed in exogenously applied plants as compared to control. In addition, improved physio-biochemical, phenological, and grain nutritional quality attributes were noticed in foliar-treated maize plots as compared to non-treated ones. SA-treated plants reduced 20% electrolyte leakage in cell membrane against control. MLE and SA were proved best in improving total phenolic, relative water (19-23%), and chlorophyll contents among other applications. A similar trend was found for photosynthetic and transpiration rates, whereas MLE and SWE were found better in improving CGR, LAI, LAD, TDM, PH, GY, grains per cob, 1000 grain weight, and biological yield among all treatments including control. TU and MLE have significantly reduced the duration in phenological events of crop at the reproductive stage. MLE, TU, and SA also improved the grain protein, oil, and starch contents as compared to control. Enhanced crop water productivity was also observed in MLE-treated plants. Economic analysis suggested that MLE and SA applications were more economical in inducing chilling stress tolerance under field conditions. Although eliciting behavior of all growth regulators improved morpho-physiological attributes against suboptimal temperature stress conditions, MLE and SA acted as leading agents which proved to be better stress alleviators by improving plant physio-biochemical attributes and maize growth.
Department of Agronomy, University of Agriculture, Faisalabad, 38040, Pakistan.
Muhammad Ahmed
MA
Waqas
Department of Agronomy, University of Agriculture, Faisalabad, 38040, Pakistan.
Imran
I
Khan
Department of Agronomy, University of Agriculture, Faisalabad, 38040, Pakistan.
Muhammad Javaid
MJ
Akhter
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, Beijing, 100081, China. mehmood2017@gmail.com.
Mehmood Ali
http://orcid.org/0000-0003-2459-2357
MA
Noor
Department of Crop science and Technology, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.|Scientific Observing and Experimental Station of Crop Cultivation in South China, Ministry of Agriculture, Guangzhou, 510642, China.
Umair
U
Ashraf
D003080:Cold Temperature; D010788:Photosynthesis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D020156:Salicylic Acid / Q000494:pharmacology; D013890:Thiourea / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects* / Q000502:physiology*
D010936:Plant Extracts; D010937:Plant Growth Regulators; D013890:Thiourea; D020156:Salicylic Acid
Exogenous application; Maize; Moringa leaf extract; Salicylic acid; Sorghum water extract; Thiourea