Transcriptome analysis of airborne PM2.5-induced detrimental effects on human keratinocytes.
Ambient air pollution is becoming more severe worldwide, posing a serious threat to human health. Fine airborne particles of particulate matter (PM2.5) show higher cytotoxicity than other coarse fractions. Indeed, PM2.5induces cardiovascular or respiratory damage; however, few studies have evaluated the detrimental effect of PM2.5to normal human skin. We used a next-generation sequencing-based (RNA-Seq) method with transcriptome and Gene Ontology (GO) enrichment analysis to determine the harmful influences of PM2.5on human normal epidermal keratinocytes. DAVID analysis showed that the most significantly enriched GO terms were associated with epidermis-related biological processes such as "epidermis development (GO: 0008544)" and "keratinocyte differentiation (GO: 0030216)", suggesting that PM2.5has some deleterious effects to the human epidermis. In addition, Ingenuity Pathway Analysis predicted inflammation-related signaling as one of the major PM2.5-induced signaling pathways, and pro-inflammatory cytokines as upstream regulators with symptoms similar to psoriasis as downstream effects. PM2.5caused considerable changes in the expression of pro-inflammatory cytokines and psoriatic skin disease-related genes, might lead to epidermal dysfunctions. Our results might help to understand the mechanism of air pollution-induced skin barrier perturbation and contribute to the development of a new strategy for the prevention or recovery of the consequent damage.
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea.
Hyoung-June
HJ
Kim
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea; College of Veterinary Medicine, Seoul National University, Seoul, 151-742 Republic of Korea.
Il-Hong
IH
Bae
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea.
Eui Dong
ED
Son
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea.
Juyearl
J
Park
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea.
Nari
N
Cha
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea.
Hye-Won
HW
Na
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea.
Changjo
C
Jung
Macrogen Inc., Seoul, 08511, Republic of Korea.
You-Seak
YS
Go
College of Veterinary Medicine, Seoul National University, Seoul, 151-742 Republic of Korea.
Dae-Yong
DY
Kim
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea. Electronic address: TRLee@amorepacific.com.
Tae Ryong
TR
Lee
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 446-729, Republic of Korea. Electronic address: biopang@amorepacific.com.
Dong Wook
DW
Shin
D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics; D004817:Epidermis / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010316:Particle Size; D052638:Particulate Matter / Q000737:chemistry / Q000633:toxicity*; D011565:Psoriasis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D059467:Transcriptome / Q000187:drug effects*
D000393:Air Pollutants; D016207:Cytokines; D052638:Particulate Matter
Human normal keratinocytes; Next-generation sequencing; Particulate matter