Chemical composition, antioxidant activity and in vitro antibacterial activity of Achillea wilhelmsii C. Koch essential oil on methicillin-susceptible and methicillin-resistant Staphylococcus aureus spp.
The present study investigated the chemical composition of the essential oil (EO) from aerial parts (flowering stage) of Achillea wilhelmsii C. Koch by GC-MS. In addition, the antioxidant activity of the EO as well as its antimicrobial activity against methicillin-susceptible and methicillin-resistant Staphylococcus aureus (MRSA) strains was tested. Antioxidant activity was measured by the ability of the EO to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals while the antimicrobial activity was assessed by the disc-diffusion method. In total, 52 compounds were recognized, accounting for 97.33 % of the EO. The main compounds in the EO were carvacrol (22.49 %), dihydrocarvone (13.23 %), linalool (12 %), 1,8-cineol (11.42 %), camphene (8.31 %), thymol (5.28 %), camphor (3.71 %), pulegone (2.82 %) α-terpineol (2.11 %), bornyl acetate (1.14 %), and farganol (1.01 %). The EC50value of the EO was 0.01 and 0.08 mg/mL for the antioxidant and DPPH-scavenging ability, respectively. A. wilhelmsii EO affected methicillin-sensitive Staphylococcus aureus (MSSA) and MRSA, but the impact was more effective on MSSA.
Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, P.O. Box 71455-119, Shiraz, Iran.
Seyedeh Mahsan Hoseini
SM
Alfatemi
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, P.O. Box 61615-585, Zabol, Iran. javad.sharifirad@gmail.com.|Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, P.O. Box 61615-585, Zabol, Iran. javad.sharifirad@gmail.com.|Cereal Health Research Center of Zabol, Zabol University of Medical Sciences, P.O. Box 61615-585, Zabol, Iran. javad.sharifirad@gmail.com.
Javad Sharifi
JS
Rad
Department of Range and Watershed Management, Faculty of Natural Resources, University of Zabol, Zabol, Iran.|Department of Rangeland Science, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739, Gorgan, Iran.
Majid Sharifi
MS
Rad
Department of Biology, College of Sciences, Shiraz University, 71454, Shiraz, Iran.
Sasan
S
Mohsenzadeh
, P.O. Box 7, Miki Cho Post Office, 3011-2, Ikenobe, Kagawa-Ken, 761-0799, Japan.
Jaime A Teixeira
JA
da Silva
Achillea wilhelmsii; Antioxidant activity; Essential oil (EO); GC–MS; MRSA; MSSA; Staphylococcus aureus