14-3-3 α and 14-3-3 ζ contribute to immune responses in planarian Dugesia japonica.
14-3-3 proteins are a family of highly conserved acidic proteins that regulate cellular processes. They act as a kind of important signaling molecules taking part in many crucial decisions throughout the development process. We have isolated and characterized two members of the 14-3-3 family, namely, Dj14-3-3 α and Dj14-3-3 ζ in the planarian Dugesia japonica. The Dj14-3-3 α and ζ genes encode polypeptides of 260 and 255 amino acids respectively. We have proved that the Dj14-3-3 α and ζ genes were especially expressed in the pharynx in adult and regenerating planarians by in situ hybridization and they were not involved in regeneration process. Besides, Dj14-3-3 α and ζ genes can compensate each other in planarians by RNA interference. The Dj14-3-3 α and ζ were significantly up-regulated expression when planarians were stimulated with the pathogen-associated molecular patterns including lipopolysaccharide (LPS), peptidoglycan (PGN), β-Glu and Poly (I:C), indicating that the Dj14-3-3 α and ζ may be involved in the immune responses.
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China; School of Life Sciences, Shandong University of Technology, 255049, China.
Qingqing
Q
Lu
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China.
Suge
S
Wu
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China.
Hui
H
Zhen
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China; School of Life Sciences, Shandong University of Technology, 255049, China.
Hongkuan
H
Deng
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China; School of Life Sciences, Shandong University of Technology, 255049, China.
Qian
Q
Song
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China; School of Life Sciences, Shandong University of Technology, 255049, China.
Kaifu
K
Ma
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China; School of Life Sciences, Shandong University of Technology, 255049, China.
Zhonghong
Z
Cao
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China; School of Life Sciences, Shandong University of Technology, 255049, China.
Qiuxiang
Q
Pang
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, 255049, China; School of Life Sciences, Shandong University of Technology, 255049, China. Electronic address: zhaobosheng@sdut.edu.cn.
Bosheng
B
Zhao
D048948:14-3-3 Proteins / Q000235:genetics* / Q000276:immunology; D000818:Animals; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000494:pharmacology; D010614:Pharynx / Q000502:physiology; D010932:Planarians / Q000235:genetics* / Q000276:immunology* / Q000502:physiology; D034622:RNA Interference; D012038:Regeneration / Q000235:genetics
D048948:14-3-3 Proteins; D015801:Helminth Proteins; D000069452:Pathogen-Associated Molecular Pattern Molecules
Dj14-3-3 α and ζ; Dugesia japonica; Expression patterns; Immunity