Clinical Relevance of Cytokines Gene Polymorphisms and Protein Levels in Gingival Cervical Fluid from Chronic Periodontitis Patients.
BACKGROUND
Cytokines are suggested to play a role in periodontitis.


OBJECTIVE
To determine and compare the levels of Interleukin-1 beta (IL-1β) and Tumor necrosis factor alpha (TNF-α) in gingival crevicular fluid (GCF) samples amongst healthy individuals and those with chronic periodontitis. Further to compare the GCF cytokine levels in three genotype classes defined by the respective gene polymorphisms.


METHODS
The study was conducted on 41 chronic periodontitis patients and 40 healthy volunteers. IL-1β and TNF-α were quantified in GCF by cytometric bead array. DNA was extracted from peripheral blood samples and genotyping of IL1B +3954C/T (rs1143634) IL1B -511G/A (rs16944), TNFA -1031T/C (rs1799964) and TNFA -863C/A (rs1800630) polymorphisms were performed using Sanger sequencing and Taqman SNP genotyping assays methods.


RESULTS
Both IL-1β and TNF-α levels were significantly higher in chronic periodontitis group compared to the controls. IL-1β and TNF-α levels did not significantly differ in genotype classes of the respective polymorphism (IL1B -511G/A, TNFA -1031T/C and TNFA -863C/A). However, individuals with CT genotype of IL1B +3954C/T showed higher levels of IL-1β in the gingival crevicular fluid (ANOVA p<0.05).


CONCLUSIONS
The results of this study revealed the presence of higher levels of IL-1β and TNF-α in subjects with periodontitis and genetic control of IL-1β levels in our samples of Indians.
Department of Periodontics, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, Tamil Nadu, India.
Vamsi
V
Lavu
Vettriselvi
V
Venkatesan
Priyanka
P
Venugopal
Bhaskar Venkata Kameswara Subrahmanya
BV
Lakkakula
Solomon Franklin Durairaj
SF
Paul
Kumarasamy
K
Peria
Suresh Ranga
SR
Rao
D055113:Chronic Periodontitis / Q000235:genetics* / Q000276:immunology; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005883:Gingival Crevicular Fluid / Q000276:immunology*; D006801:Humans; D007194:India; D053583:Interleukin-1beta / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha