Ammonium assimilation: An important accessory during aerobic denitrification of Pseudomonas stutzeri T13.
The present study investigated effect of ammonium utilization on aerobic denitrification by Pseudomonas stutzeri T13. Per nitrogen balance calculation, all consumed ammonium was utilized as nitrogen source for cell propagation by assimilation rather than heterotrophic nitrification. Total organic carbon (TOC) and ammonium were necessary substrates to sustain heterotrophic propagation of P. stutzeri T13 at optimum proportion equal to seven. Under aerobic condition, nitrate was utilized as substitute nitrogen source when ammonium was completely exhausted. Biomass production effectively increased with increasing initial ammonium from 0mg/L to 100mg/L. Owing to enlarged biomass, average nitrate reduction rate increased from 7.36mgL-1h-1to 11.95mgL-1h-1. Such process also successfully reduced nitrite accumulation from 121.8mg/L to 66.16mg/L during aerobic denitrification. As important accessory during aerobic denitrification, ammonium assimilation efficiently doubled total nitrogen (TN) removal from 54.97mg/L (no ammonium provided) to 113.1mg/L (100mg/L ammonium involved).
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Yilu
Y
Sun
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Liang
L
Feng
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China. Electronic address: ang.li.harbin@gmail.com.
Ang
A
Li
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Xuening
X
Zhang
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Jixian
J
Yang
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Fang
F
Ma
D000332:Aerobiosis; D064751:Ammonium Compounds; D058440:Denitrification; D058465:Nitrification; D009584:Nitrogen; D044223:Pseudomonas stutzeri
D064751:Ammonium Compounds; D009584:Nitrogen
Aerobic denitrification; Ammonium assimilation; Heterotrophic nitrification; Pseudomonas stutzeri T13; TN removal