High-throughput identification of miRNAs of Taenia ovis, a cestode threatening sheep industry.
Taenia ovis is a tapeworm that is mainly transmitted between dogs and sheep or goats and has an adverse effect on sheep industry. miRNAs are short regulatory non-coding RNAs, involved in parasite development and growth as well as parasite infection. The miRNA profile of T. ovis remains to be established. Herein, 33 known miRNAs belonging to 23 different families were identified in T. ovis metacestodes using deep sequencing approach. Of them, expression of some miRNAs such as tov-miR-10 and -let-7 was absolutely predominant. Moreover, comparative analysis revealed the presence of a miR-71/2b/2c cluster in T. ovis, which was also completely conserved in other 6 cestodes. The study provides rich data for further understandings of T. ovis biology.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: zhengyadong@caas.cn.
Yadong
Y
Zheng
D000822:Animal Husbandry; D000818:Animals; D001483:Base Sequence; D003551:Cysticercosis / Q000469:parasitology / Q000662:veterinary*; D004285:Dogs; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000145:classification / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D018510:RNA, Helminth / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D034561:Sheep, Domestic; D013621:Taenia / Q000235:genetics* / Q000302:isolation & purification
D035683:MicroRNAs; D018510:RNA, Helminth
Taenia ovis; cestode; miRNA