Complete genome sequences of cowpea polerovirus 1 and cowpea polerovirus 2 infecting cowpea plants in Burkina Faso.
The full-length genome sequences of two novel poleroviruses found infecting cowpea plants, cowpea polerovirus 1 (CPPV1) and cowpea polerovirus 2 (CPPV2), were determined using overlapping RT-PCR and RACE-PCR. Whereas the 5845-nt CPPV1 genome was most similar to chickpea chlorotic stunt virus (73% identity), the 5945-nt CPPV2 genome was most similar to phasey bean mild yellow virus (86% identity). The CPPV1 and CPPV2 genomes both have a typical polerovirus genome organization. Phylogenetic analysis of the inferred P1-P2 and P3 amino acid sequences confirmed that CPPV1 and CPPV2 are indeed poleroviruses. Four apparently unique recombination events were detected within a dataset of 12 full polerovirus genome sequences, including two events in the CPPV2 genome. Based on the current species demarcation criteria for the family Luteoviridae, we tentatively propose that CPPV1 and CPPV2 should be considered members of novel polerovirus species.
Laboratoire Biosciences, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR-SVT), Université Ouaga I Pr Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou, Burkina Faso.|Laboratoire de Virologie et de Biotechnologies Végétales, INERA, 01 BP 476, Ouagadougou, Burkina Faso.|CIRAD-INRA-SupAgro, UMR BGPI, 34398, Montpellier, France.|LMI Patho-Bios, 01 BP 476, Ouagadougou, Burkina Faso.
Essowè
E
Palanga
Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa.
Darren P
DP
Martin
CIRAD-INRA-SupAgro, UMR BGPI, 34398, Montpellier, France.
Serge
S
Galzi
Laboratoire de Virologie et de Biotechnologies Végétales, INERA, 01 BP 476, Ouagadougou, Burkina Faso.|LMI Patho-Bios, 01 BP 476, Ouagadougou, Burkina Faso.
Jean
J
Zabré
Laboratoire de Virologie et de Biotechnologies Végétales, INERA, 01 BP 476, Ouagadougou, Burkina Faso.|LMI Patho-Bios, 01 BP 476, Ouagadougou, Burkina Faso.
Zakaria
Z
Bouda
Laboratoire de Virologie et de Biotechnologies Végétales, INERA, 01 BP 476, Ouagadougou, Burkina Faso.|LMI Patho-Bios, 01 BP 476, Ouagadougou, Burkina Faso.
James Bouma
JB
Neya
Laboratoire de Virologie et de Biotechnologies Végétales, INERA, 01 BP 476, Ouagadougou, Burkina Faso.
Mahamadou
M
Sawadogo
Laboratoire de Virologie et de Biotechnologies Végétales, INERA, 01 BP 476, Ouagadougou, Burkina Faso.|LMI Patho-Bios, 01 BP 476, Ouagadougou, Burkina Faso.
Oumar
O
Traore
CIRAD-INRA-SupAgro, UMR BGPI, 34398, Montpellier, France.
Michel
M
Peterschmitt
CIRAD-INRA-SupAgro, UMR BGPI, 34398, Montpellier, France. philippe.roumagnac@cirad.fr.
Philippe
P
Roumagnac
CIRAD-INRA-SupAgro, UMR BGPI, 34398, Montpellier, France.
Denis
D
Filloux
D002050:Burkina Faso; D016679:Genome, Viral; D052679:Luteoviridae / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D000070658:Vigna / Q000821:virology*
D012367:RNA, Viral