Molecular modeling and expression analysis of a MADS-box cDNA from mango (Mangifera indica L.).
MADS-box genes are a large family of transcription factors initially discovered for their role during development of flowers and fruits. The MADS-box transcription factors from animals have been studied by X-ray protein crystallography but those from plants remain to be studied. In this work, a MADS-box cDNA from mango encoding a protein of 254 residues was obtained and compared. Based on phylogenetic analysis, it is proposed that the MADS-box transcription factor expressed in mango fruit (MiMADS1) belongs to the SEP clade of MADS-box proteins. MiMADS1 mRNA steady-state levels did not changed during mango fruit development and were up-regulated, when mango fruits reached physiological maturity as assessed by qRT-PCR. Thus, MiMADS1 could have a role during development and ripening of this fruit. The theoretical structural model of MiMADS1 showed the DNA-binding domain folding bound to a double-stranded DNA. Therefore, MiMADS1 is an interesting model for understanding DNA-binding for transcriptional regulation.
Plant Molecular Biology Lab, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico.
Magda A
MA
Pacheco-Sánchez
Plant Molecular Biology Lab, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico.
Carmen A
CA
Contreras-Vergara
Plant Molecular Biology Lab, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico.
Eduardo
E
Hernandez-Navarro
Plant Molecular Biology Lab, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico.
Gloria
G
Yepiz-Plascencia
Plant Molecular Biology Lab, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico.
Miguel A
MA
Martínez-Téllez
División de Biología Molecular, IPICYT, Camino a la Presa San José No. 2055, Lomas 4a sección, 78216, San Luis Potosí, Mexico.
Sergio
S
Casas-Flores
Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Luis Encinas y Blvd. Rosales S/N, 83000, Hermosillo, Sonora, Mexico.
Aldo A
AA
Arvizu-Flores
Plant Molecular Biology Lab, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico. islasosu@ciad.mx.
Maria A
MA
Islas-Osuna
Gene expression; MADS-box; Mangifera indica L.; Molecular modeling; Transcription factor