Treatment ofLeishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-KilledPropionibacterium acnesTriggers Protective Cellular Immune Responses.
Palladacycle complex DPPE 1.2 was previously reported to inhibit thein vitroandin vivoinfection byLeishmania (Leishmania) amazonensis. The aim of the present study was to compare the effect of DPPE 1.2, in association with heat-killedPropionibacterium acnes, onL. (L.) amazonensisinfection in two mouse strains, BALB/c and C57BL/6, and to evaluate the immune responses of the treated animals. Foot lesions ofL. (L.) amazonensis-infected mice were injected with DPPE 1.2 alone, or associated withP.acnesas an adjuvant. Analysis of T-cell populations in the treated mice and in untreated controls was performed by FACS. Detection of IFN-γ-secreting lymphocytes was carried out by an ELISPOT assay and active TGF-β was measured by means of a double-sandwich ELISA test. The treatment with DPPE 1.2 resulted in a significant reduction of foot lesion sizes and parasite burdens in both mouse strains, and the lowest parasite burden was found in mice treated with DPPE 1.2 plusP. acnes. Mice treated with DPPE 1.2 alone displayed a significant increase of TCD4+and TCD8+lymphocytes and IFN-γ secretion which were significantly higher in animals treated with DPPE 1.2 plusP. acnes. A significant reduction of active TGF-β was observed in mice treated with DPPE 1.2 alone or associated withP. acnes. Moreover, DPPE 1.2 associated toP. acneswas non-toxic to treated animals. The destruction ofL. (L.) amazonensisby DPPE 1.2 was followed by host inflammatory responses which were exacerbated when the palladacycle complex was associated withP. acnes.
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Carolina S
CS
Paladi
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Danielle A M
DA
da Silva
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Priscila D
PD
Motta
Departamento de Farmacologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Daniel M
DM
Garcia
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Daniela
D
Teixeira
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Ieda M
IM
Longo-Maugéri
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Simone
S
Katz
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil.
Clara L
CL
Barbiéri
Leishmania (Leishmania) amazonensis; Propionibacterium acnes; adjuvant; cutaneous leishmaniasis; palladacycle complex