Influence of thylakoid membrane lipids on the structure of aggregated light-harvesting complexes of the diatom Thalassiosira pseudonana and the green alga Mantoniella squamata.
The study investigated the effect of the thylakoid membrane lipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG) on the structure of two algal light-harvesting complexes (LHCs). In contrast to higher plants whose thylakoid membranes are characterized by an enrichment of the neutral galactolipids MGDG and DGDG, both the green alga Mantoniella squamata and the centric diatom Thalassiosira pseudonana contain membranes with a high content of the negatively charged lipids SQDG and PG. The algal thylakoids do not show the typical grana-stroma differentiation of higher plants but a regular arrangement. To analyze the effect of the membrane lipids, the fucoxanthin chlorophyll protein (FCP) complex of T. pseudonana and the LHC of M. squamata (MLHC) were prepared by successive cation precipitation using Triton X-100 as detergent. With this method, it is possible to isolate LHCs with a reduced amount of associated lipids in an aggregated state. The results from 77 K fluorescence and photon correlation spectroscopy show that neither the neutral galactolipids nor the negatively charged lipids are able to significantly alter the aggregation state of the FCP or the MLHC. This is in contrast to higher plants where SQDG and PG lead to a strong disaggregation of the LHCII whereas MGDG and DGDG induce the formation of large macroaggregates. The results indicate that LHCs which are integrated into thylakoid membranes with a high amount of negatively charged lipids and a regular arrangement are less sensitive to lipid-induced structural alterations than their counterparts in membranes enriched in neutral lipids with a grana-stroma differentiation.
Institute of Biology, University of Leipzig, Leipzig, D-04103, Germany.
Susann
S
Schaller-Laudel
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, 30-387, Poland.
Dariusz
D
Latowski
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, 30-387, Poland.
Małgorzata
M
Jemioła-Rzemińska
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, 30-387, Poland.|Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, 30-387, Poland.
Kazimierz
K
Strzałka
Institute of Chemistry, Martin-Luther-University Halle-Wittenberg, Halle, D-06120, Germany.
Sebastian
S
Daum
Institute of Chemistry, Martin-Luther-University Halle-Wittenberg, Halle, D-06120, Germany.
Kirsten
K
Bacia
Institute of Biology, University of Leipzig, Leipzig, D-04103, Germany.
Christian
C
Wilhelm
Institute of Biology, University of Leipzig, Leipzig, D-04103, Germany.
Reimund
http://orcid.org/0000-0001-9347-8158
R
Goss
D002734:Chlorophyll / Q000378:metabolism; D061146:Chlorophyll Binding Proteins / Q000378:metabolism; D017377:Diatoms / Q000378:metabolism*; D038983:Galactolipids / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism*
D061146:Chlorophyll Binding Proteins; D038983:Galactolipids; D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; D002734:Chlorophyll