Calcium phosphate-quercetin nanocomposite (CPQN): A multi-functional nanoparticle having pH indicating, highly fluorescent and anti-oxidant properties.
Calcium phosphate quercetin nanocomposite (CPQN) i.e., quercetin entrapped in calcium phosphate nanoparticle was synthesized by a precipitation method at 80°C, taking ammonium hydrogen phosphate, calcium nitrate and quercetin as precursors and sodium citrate as stabilizer. The nanocomposite suspension had different color at different pH values, a property that could render the nanoparticle a pH indicator. Besides color, the particles also had different size, shape, stability and quercetin content with change of pH. In addition, the CPQN was highly fluorescent having two sharp emission peaks at 460 and 497nm, when excited at 370nm; by this property it behaved as an effective fluorophore to label biological cell. Moreover, the nanocomposite had potential anti-oxidant property, for which mortality of mouse neuroblastoma cell N2A, by H2O2-induced oxidative stress, was found to be lowered by the pre-treatment of the cells with CPQN.
Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741 235, West Bengal, India.
Mousumi
M
Patra
Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741 235, West Bengal, India.
Riya
R
Mukherjee
Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741 235, West Bengal, India.
Milon
M
Banik
Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741 235, West Bengal, India.
Debanjan
D
Dutta
Department of Chemistry, Viswa Bharati University, Santiniketan, West Bengal, India.
Naznin Ara
NA
Begum
Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741 235, West Bengal, India. Electronic address: tarakdb@yahoo.com.
Tarakdas
T
Basu
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D036103:Nanotechnology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010316:Particle Size; D011794:Quercetin / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology
D000975:Antioxidants; D002130:Calcium Phosphates; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011794:Quercetin
Antioxidant; Calcium phosphate nanoparticle; Fluorophore; Quercetin; pH-sensitive properties