Nutrient improvement for simultaneous production of exopolysaccharide and mycelial biomass by submerged cultivation of Schizophyllum commune AGMJ-1 using statistical optimization.
Exopolysaccharides (EPS) of fungal origin have attracted special attention from researchers due to their multifarious applications in the food and pharmaceutical industries. In the present study, optimization of the process parameters for the production of exopolysaccharide by Schizophyllum commune AGMJ-1 was studied using one factor at a time (OFAT) method, Plackett-Burman design (PBD) and response surface methodology (RSM). OFAT method revealed xylose and yeast extract to be the most effective carbon and nitrogen sources and pH 5.3 as an optimum for maximum EPS production. Xylose, yeast extract and KCl were screened as statistically significant variables for EPS production using PBD. RSM based on the central composite design estimated that maximum EPS (4.26 g L-1), mycelial biomass (14 g L-1) and specific yield (0.45 g g-1) were obtained when concentration of xylose, yeast extract and KCl were set at 2.5 g % (w/v), 0.83 g % (w/v) and 6.53 mg % (w/v), respectively, in the production medium.
Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, 388 120, Gujarat, India.
Mayur
M
Joshi
Department of Microbiology, VP and RPTP Science College, Vallabh Vidyanagar, 388 120, Gujarat, India.
Harshad
H
Patel
Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidyanagar, 388 121, Gujarat, India.
Shilpa
S
Gupte
Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, 388 120, Gujarat, India. akshaya_gupte@hotmail.com.
Akshaya
A
Gupte
Exopolysaccharide; Mycelial biomass; One factor at a time; Plackett–Burman design; Response surface methodology; Schizophyllum commune