Significant Role of Collagen XVII And Integrin β4 in Migration and Invasion of The Less Aggressive Squamous Cell Carcinoma Cells.
Collagen XVII and integrin α6β4 have well-established roles as epithelial adhesion molecules. Their binding partner laminin 332 as well as integrin α6β4 are largely recognized to promote invasion and metastasis in various cancers, and collagen XVII is essential for the survival of colon and lung cancer stem cells. We have studied the expression of laminin γ2, collagen XVII and integrin β4 in tissue microarray samples of squamous cell carcinoma (SCC) and its precursors, actinic keratosis and Bowen's disease. The expression of laminin γ2 was highest in SCC samples, whereas the expression of collagen XVII and integrin β4 varied greatly in SCC and its precursors. Collagen XVII and integrin β4 were also expressed in SCC cell lines. Virus-mediated RNAi knockdown of collagen XVII and integrin β4 reduced the migration of less aggressive SCC-25 cells in horizontal scratch wound healing assay. Additionally, in a 3D organotypic myoma invasion assay the loss of collagen XVII or integrin β4 suppressed equally the migration and invasion of SCC-25 cells whereas there was no effect on the most aggressive HSC-3 cells. Variable expression patterns and results in migration and invasion assays suggest that collagen XVII and integrin β4 contribute to SCC tumorigenesis.
Department of Dermatology, PEDEGO Research Unit, Oulu Center for Cell-Matrix Research, MRC Oulu, University of Oulu and Oulu University Hospital, Finland.
Jyri M
JM
Moilanen
Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University of Duisburg-Essen, Germany.
Stefanie
S
Löffek
Department of Dermatology, PEDEGO Research Unit, Oulu Center for Cell-Matrix Research, MRC Oulu, University of Oulu and Oulu University Hospital, Finland.
Nina
N
Kokkonen
Oulu Center for Cell-Matrix Research, Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland.
Sirpa
S
Salo
Department of Pathology, Research Unit of Cancer and Translational Medicine, MRC Oulu, University of Oulu and Oulu University Hospital, Oulu, Finland.
Juha P
JP
Väyrynen
Department of Dermatology, PEDEGO Research Unit, Oulu Center for Cell-Matrix Research, MRC Oulu, University of Oulu and Oulu University Hospital, Finland.
Tiina
T
Hurskainen
Oulu Center for Cell-Matrix Research, Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland.
Aki
A
Manninen
Department of Dermatology, Turku University Hospital, MediCity Research Laboratory, University of Turki, Turku, Finland.
Pilvi
P
Riihilä
Oulu Center for Cell-Matrix Research, Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland.
Ritva
R
Heljasvaara
Department of Dermatology, Medical Center, University of Freiburg, Freiburg, Germany.
Claus-Werner
CW
Franzke
Department of Dermatology, Turku University Hospital, MediCity Research Laboratory, University of Turki, Turku, Finland.
Veli-Matti
VM
Kähäri
Research Unit of Cancer and Translational Medicine, MRC Oulu, University of Oulu and Oulu University Hospital, Finland.|Department of Oral and Maxillo-facial Diseases, University of Helsinki, Finland.|HUSLAB, Department of Pathology, Helsinki University Central Hospital, Finland.|Department of Oral Diagnosis, Oral Pathology Division, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, SP-13414-903, Brazil.
Tuula
T
Salo
Department of Pathology, Research Unit of Cancer and Translational Medicine, MRC Oulu, University of Oulu and Oulu University Hospital, Oulu, Finland.
Markus J
MJ
Mäkinen
Department of Dermatology, PEDEGO Research Unit, Oulu Center for Cell-Matrix Research, MRC Oulu, University of Oulu and Oulu University Hospital, Finland.
Kaisa
K
Tasanen
D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D001913:Bowen's Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D055786:Gene Knockout Techniques; D006801:Humans; D039663:Integrin beta4 / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D051379:Mice; D024041:Non-Fibrillar Collagens / Q000378:metabolism*; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000097805:Collagen Type XVII
D001324:Autoantigens; D039663:Integrin beta4; D007797:Laminin; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII