Enzymatic incorporation of caffeoyl into castor oil to prepare the novel castor oil-based caffeoyl structured lipids.
In this work, a novel castor oil-based caffeoyl structured lipids was successfully prepared by the enzymatic transesterification using castor oil (CO) as caffeoyl acceptor. During the structured lipids preparation, two competitive reactions, the hydrolysis of CO to form hydrophilic caffeoyl glycerols (CG)+dicaffeoyl glycerols (DCG) and the transesterification of CO with ethyl caffeate (EC) to form lipophilic caffeoyl mono- and di-acylglycerols (CMAG and CDAG), were found. Reaction progress was monitored using HPLC-ESI-MS and HPLC-UV. The effects of by-product ethanol removal and reaction variables on the transesterification and reaction selectivity were evaluated. Results showed that, the activation energies for the transesterification and for the selective formations of CMAG+CDAG and CG+DCG were 57.60kJ/mol, 58.86kJ/mol, and 60.53kJ/mol, respectively. Under the optimal reaction conditions (enzyme load 23%, 90°C, 1:3 molar ratio of EC to CO, and 46.5h), EC conversion and the yield of CMAG+CDAG were 93.68±2.52% and 78.11±1.35%, respectively.
Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology, Lianhua Road 100, Zhengzhou 450001, Henan Province, PR China. Electronic address: sunshangde@hotmail.com.
Shangde
S
Sun
Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology, Lianhua Road 100, Zhengzhou 450001, Henan Province, PR China. Electronic address: 1451520443@qq.com.
Ping
P
Wang
Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology, Lianhua Road 100, Zhengzhou 450001, Henan Province, PR China. Electronic address: 732006138@qq.com.
Sha
S
Zhu
D001709:Biotechnology; D002109:Caffeic Acids / Q000737:chemistry* / Q000378:metabolism*; D002368:Castor Oil / Q000737:chemistry* / Q000378:metabolism*; D004800:Enzymes, Immobilized; D004951:Esterification; D000431:Ethanol; D005656:Fungal Proteins; D005989:Glycerides / Q000737:chemistry* / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism
D002109:Caffeic Acids; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005989:Glycerides; D000431:Ethanol; C032773:ethyl caffeate; D002368:Castor Oil; C093667:Novozyme 435; D008049:Lipase
Caffeic acid; Castor oil; Castor oil-based caffeoyl structured lipids; Lipase; Reaction selectivity; Transesterification