Apoptosis-inducing activity and antiproliferative effect of Paeoniflorigenone from moutan cortex.
Ninety samples from the extracts of plants from traditional Chinese medicines were screened for antitumor activity. Paeoniflorigenone (PFG) was isolated as an active ingredient from the root of moutan cortex, which showed the strongest activity. In addition, our data indicated that PFG was cytotoxic and induced apoptosis selectively in the cancer cell lines. These effects were cancelled by the addition of caspase inhibitor Z-VAD-FMK, suggesting that it was mediated by caspase-3 activation.
a Department of Biosciences & Informatics , Keio University , Yokohama , Japan.
Ying
Y
Huang
b Department of Chemistry and Life Science , School of Advanced Engineering, Kogakuin University , Hachioji , Japan.
Osamu
O
Ohno
c Department of Chemistry , Keio University , Yokohama , Japan.
Kiyotake
K
Suenaga
a Department of Biosciences & Informatics , Keio University , Yokohama , Japan.
Kenji
K
Miyamoto
D041721:3T3-L1 Cells; D000590:Amino Acid Chloromethyl Ketones / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D061945:Caspase Inhibitors / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000302:isolation & purification / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D015870:Gene Expression; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008516:Medicine, Chinese Traditional; D051379:Mice; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
D000590:Amino Acid Chloromethyl Ketones; D000972:Antineoplastic Agents, Phytogenic; D061945:Caspase Inhibitors; D003603:Cytotoxins; D004365:Drugs, Chinese Herbal; D039821:Monoterpenes; D010936:Plant Extracts; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; C038881:moutan cortex; C042713:paeoniflorigenone; C505407:CASP3 protein, human; D053148:Caspase 3
Paeonia suffruticosa Andr.; Paeoniflorigenone; antiproliferative activity; apoptosis; caspase-3 activation