Large-Scale Preparation of a Specific Xanthone from Swertia mussotii and Evaluation of Its α-Glucosidase Inhibitory Activity.
Large-scale preparation and α-glucosidase inhibitory activity of a specific xanthone swertioside from Swertia mussotii were investigated in this study. Firstly, an efficient method was successfully established by liquid-liquid extraction, preparative high-performance liquid chromatography and sephadex LH-20 for large-scale preparation of swertioside. The recovery of swertioside reached 92.0%. Secondly, in vitro α-glucosidase inhibition experiment showed that swertioside had good inhibition close to acarbose. The study showed that swertioside had potential use as an anti-diabetic agent.
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.|Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.
Tao
T
Chen
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.|Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.|Savaid Medical School, University of the Chinese Academy of Sciences, No.19, Yuquan Road, Beijing 100049, P.R. China.
Hongmei
H
Li
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.|Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.
Chen
C
Chen
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.|Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.
Lixin
L
Wei
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.|Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No.23, Xinning Road, Xining 810008, P.R. China.
Yulin
Y
Li
D002851:Chromatography, High Pressure Liquid; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D059627:Liquid Phase Microextraction; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D029967:Swertia / Q000737:chemistry*; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D000520:alpha-Glucosidases / Q000378:metabolism
D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D044004:Xanthones; D000520:alpha-Glucosidases