Liquid Chromatography with mass spectrometry analysis of mycotoxins in food samples using silica hydride based stationary phases.
Liquid chromatography with mass spectrometry analysis of selected food samples using silica hydride stationary phases allowed for the identification and quantification of common mycotoxins including aflatoxin B1, B2, G1, G2, ochratoxin A, and fumosinin B1. Phenyl and C18columns showed relatively similar selectivity based on hydrophobicity but the phenyl phase provides an additional mechanism, π-π interaction. The most hydrophobic of the analyzed compounds was more strongly retained on the C18column and also has fewer unsaturated sites, which limited the interaction with the phenyl phase. Bean, maize, rice, and wheat samples were harvested and stored under conditions conducive to fungal development, and all samples presented toxin contamination exceeding the maximum tolerable limits.
Department of Chemistry, San Jose State University, San Jose, CA, USA.
Joseph J
JJ
Pesek
Department of Chemistry, San Jose State University, San Jose, CA, USA.
Maria T
MT
Matyska
Department of Agroindustrial Science and Technology, Federal University of Pelotas, RS, Brazil.
Jessica F
JF
Hoffmann
Department of Agroindustrial Science and Technology, Federal University of Pelotas, RS, Brazil.
Nathalia A
NA
Madruga
Department of Agroindustrial Science and Technology, Federal University of Pelotas, RS, Brazil.
Rosane L
RL
Crizel
Department of Agroindustrial Science and Technology, Federal University of Pelotas, RS, Brazil.
Moacir C
MC
Elias
Department of Agroindustrial Science and Technology, Federal University of Pelotas, RS, Brazil.
Nathan L
NL
Vanier
Department of Agroindustrial Science and Technology, Federal University of Pelotas, RS, Brazil.
Fabio C
FC
Chaves
D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis*; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis*; D017640:Silicates
D009183:Mycotoxins; D017640:Silicates; C487012:silica hydride
aflatoxin; fumosinin; liquid chromatography-mass spectrometry; ochratoxin; phenyl stationary phases