Up-regulation of miR-888-5p in hepatocellular carcinoma cell lines and its effect on malignant characteristics of cells.
MicroRNA (miRNA) expression has been linked to the molecular pathogenesis of hepatocellular carcinoma (HCC). The aberrant expression of miRNA is involved in the processes of tumorigenesis and cancer progression. According to the latest research, miR-888-5p is associated with strong cancer-promoting effect. For instance, miR-888-5p is up-regulated in prostate cancer and breast cancer. Nevertheless, the role of miR-888-5p in HCC has not been investigated to date. In this study, we found that miR-888-5p levels in four HCC cell lines (SMMC7721, HepG2, Huh-7 and Bel7402) were significantly up-regulated compared with human hepatocyte cell line (HHL-5). After transiently transfected with miR-888-5p mimic, our results demonstrated that miR-888-5p plays a major role in promoting the proliferation and metastatic potential of HCC cells. Moreover, miR-888-5p also increased the expression of MMP-2 and MMP-9 proteins which account for cell migration and invasion, and decreased the expression of p53 protein which further promoted malignance of HCC. Therefore, miR-888-5p may be considered a potential biomarker for diagnostics and prognosis of HCC.
Department of General Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.
E
E
Hao
Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin, China.
J
J
Yu
Department of General Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.
S
S
Xie
Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin, China.
W
W
Zhang
Department of General Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.
G
G
Wang
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D020320:Oligoribonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
D014408:Biomarkers, Tumor; C000589534:MIRN888 microRNA, human; D035683:MicroRNAs; D020320:Oligoribonucleotides, Antisense; D016159:Tumor Suppressor Protein p53; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9