Monitoring Mitophagy During Aging in Caenorhabditis elegans.
Mitochondria constitute the main energy-producing centers of eukaryotic cells. In addition, they are involved in several crucial cellular processes, such as lipid metabolism, calcium buffering, and apoptosis. As such, their malfunction can be detrimental for proper cellular physiology and homeostasis. Mitophagy is a mechanism that protects and maintains cellular function by sequestering harmful or dysfunctional mitochondria to lysosomes for degradation. In this report, we present experimental procedures for quantitative, in vivo monitoring of mitophagy events in the nematode Caenorhabditis elegans.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Vassilika Vouton, Heraklion, Crete, Greece.|Department of Biology, University of Crete, Heraklion, 70013, Crete, Greece.
Nikolaos
N
Charmpilas
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Vassilika Vouton, Heraklion, Crete, Greece.|Department of Biology, University of Crete, Heraklion, 70013, Crete, Greece.
Konstantinos
K
Kounakis
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Vassilika Vouton, Heraklion, Crete, Greece. tavernarakis@imbb.forth.gr.|Department of Basic Sciences, Faculty of Medicine, University of Crete, Heraklion, 71305, Greece. tavernarakis@imbb.forth.gr.
Nektarios
N
Tavernarakis
D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000071182:Autophagosomes / Q000378:metabolism; D001681:Biological Assay; D015374:Biosensing Techniques; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D063306:Mitophagy
D029742:Caenorhabditis elegans Proteins
Aging; Autophagy; Caenorhabditis elegans; Mitochondria; Mitophagy; Ratiometric imaging; Rosella biosensor