A plant-derived dehydrorotenoid: a new inhibitor of hepatitis C virus entry.
Emergence of drug-resistant viruses, high cost and adverse side-effects associated with the standard therapy against hepatitis C virus (HCV) infection demonstrate the need for development of well tolerated and effective antivirals. We identified and chemically characterised the dehydrorotenoid boeravinone H, isolated from the herb Boerhavia diffusa, as a new inhibitor of HCV entry. The compound significantly inhibits the binding and entry of hepatitis C-like particles (HCV-LPs) in hepatoma cells in vitro with no apparent cytotoxicity. Boeravinone H inhibits the initial phase of HCV entry probably by acting directly on the viral particle. Importantly, the compound prevents HCV entry and infection in cell culture (ex vivo). Thus, boeravinone H is a potential antiviral agent for the prevention and control of HCV infection.
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Mihika
M
Bose
Department of Organic Chemistry, Indian Institute of Science, Bangalore, India.
Mohini
M
Kamra
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Ranajoy
R
Mullick
Department of Organic Chemistry, Indian Institute of Science, Bangalore, India.|Director's Research Unit, Indian Association for the Cultivation of Science, Kolkata, India.
Santanu
S
Bhattacharya
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Saumitra
S
Das
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Anjali A
AA
Karande
D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000821:virology; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D039281:Nyctaginaceae / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D053586:Virus Internalization / Q000187:drug effects*
D005419:Flavonoids; D010936:Plant Extracts; C541867:dehydrorotenone
antivirals; dehydrorotenoid; entry inhibitor; hepatitis C virus; phytochemicals; virus-like particles