Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil.
The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy. The ESI-MS analysis exhibited the major polyhydroxybutyrate with a molecular mass of m/z 448.5.
Chemistry Research Laboratory, National Textile University, Faisalabad, 37610, Pakistan.
Sharjeel
S
Abid
Chemistry Research Laboratory, National Textile University, Faisalabad, 37610, Pakistan. zarazapk@yahoo.com.
Zulfiqar Ali
ZA
Raza
Chemistry Research Laboratory, National Textile University, Faisalabad, 37610, Pakistan.
Tanveer
T
Hussain
PHAs; Polyhydroxyalkanoates; Pseudomonas aeruginosa; Shake flask; Soybean oil