JcDREB2, a Physic Nut AP2/ERF Gene, Alters Plant Growth and Salinity Stress Responses in Transgenic Rice.
Transcription factors of the AP2/ERF family play important roles in plant growth, development, and responses to biotic and abiotic stresses. In this study, a physic nut AP2/ERF gene,JcDREB2, was functionally characterized. Real-time PCR analysis revealed thatJcDREB2was expressed mainly in the leaf and could be induced by abscisic acid but suppressed by gibberellin (GA) and salt. Transient expression of a JcDREB2-YFP fusion protein in Arabidopsis protoplasts cells suggested that JcDREB2 is localized in the nucleus. Rice plants overexpressingJcDREB2exhibited dwarf and GA-deficient phenotypes with shorter shoots and roots than those of wild-type plants. The dwarfism phenotype could be rescued by the application of exogenous GA3. The expression levels of GA biosynthetic genes includingOsGA20ox1,OsGA20ox2,OsGA20ox4,OsGA3ox2, OsCPS1,OsKO2, andOsKAOwere significantly reduced in plants overexpressingJcDREB2. Overexpression ofJcDREB2in rice increased sensitivity to salt stress. Increases in the expression levels of several salt-tolerance-related genes in response to salt stress were impaired inJcDREB2-overexpressing plants. These results demonstrated not only thatJcDREB2influences GA metabolism, but also that it can participate in the regulation of the salt stress response in rice.
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Yuehui
Y
Tang
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Kun
K
Liu
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Ju
J
Zhang
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Xiaoli
X
Li
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Kedong
K
Xu
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Yi
Y
Zhang
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Jing
J
Qi
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Deshui
D
Yu
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Jian
J
Wang
Henan Key Laboratory of Crop Molecular Breeding and BioreactorZhoukou, China; Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal UniversityZhoukou, China.
Chengwei
C
Li
AP2/ERF transcription factor; gibberellin; physic nut (Jatropha curcas L.); rice; salinity