Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway.
Agaricus blazei Murill (ABM) is a common anticancer folk remedy. Its active ingredients, i.e., polysaccharides, have been isolated and exhibit indirect tumor-suppressing activity via immunological activation. The effects of polysaccharides derived from A. blazei Murill (ABMP) on RAW 264.7 cells were examined by western blotting and real-time reverse transcription polymerase chain reaction (RT-PCR). The effects of 500, 1000, and 2000 μg mL-1ABMP on the growth of RAW 264.7 cells were evaluated by measuring the OD490value; the optimum concentration was found to be 1000 μg mL-1. Based on the RT-PCR results, the expression levels of JNK, ERK, and p38 decreased substantially in lipopolysaccharide (LPS)-induced RAW 264.7 cells treated with ABMP. In RAW 264.7 cells treated with LPS, the protein expression levels of JNK, ERK, and p38 were decreased, as were the levels of phosphorylated JNK, ERK, and p38. These results indicate that the MAPK signal transduction pathway is a potential mechanism by which ABMP regulates the cell-mediated immunity of RAW 264.7 cells.
Food Science and Engineering, Shanxi Agricultural University, Taigu, 030801, People's Republic of China.
Feier
F
Cheng
Xiaoyan
X
Yan
Miaoqing
M
Zhang
Mingchang
M
Chang
Shaojun
S
Yun
Junlong
J
Meng
Jingyu
J
Liu
Cui-Ping
CP
Feng
D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D007111:Immunity, Cellular / Q000187:drug effects*; D048670:MAP Kinase Kinase 4 / Q000235:genetics / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D014675:Vegetables / Q000737:chemistry; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000276:immunology
D010936:Plant Extracts; D011134:Polysaccharides; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4