Genome sequence of pacific abalone (Haliotis discus hannai): the first draft genome in family Haliotidae.
Abalones are large marine snails in the family Haliotidae and the genus Haliotis belonging to the class Gastropoda of the phylum Mollusca. The family Haliotidae contains only one genus, Haliotis, and this single genus is known to contain several species of abalone. With 18 additional subspecies, the most comprehensive treatment of Haliotidae considers 56 species valid [ 1 ]. Abalone is an economically important fishery and aquaculture animal that is considered a highly prized seafood delicacy. The total global supply of abalone has increased 5-fold since the 1970s and farm production increased explosively from 50 mt to 103 464 mt in the past 40 years. Additionally, researchers have recently focused on abalone given their reported tumor suppression effect. However, despite the valuable features of this marine animal, no genomic information is available for the Haliotidae family and related research is still limited. To construct the H . discus hannai genome, a total of 580-G base pairs using Illumina and Pacbio platforms were generated with 322-fold coverage based on the 1.8-Gb estimated genome size of H . discus hannai using flow cytometry. The final genome assembly consisted of 1.86 Gb with 35 450 scaffolds (>2 kb). GC content level was 40.51%, and the N50 length of assembled scaffolds was 211 kb. We identified 29 449 genes using Evidence Modeler based on the gene information from ab initio prediction, protein homology with known genes, and transcriptome evidence of RNA-seq. Here we present the first Haliotidae genome, H . discus hannai , with sequencing data, assembly, and gene annotation information. This will be helpful for resolving the lack of genomic information in the Haliotidae family as well as providing more opportunities for understanding gastropod evolution.
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Bo-Hye
BH
Nam
Interdisciplinary Program in Bioinformatics, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, Republic of Korea.|C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Woori
W
Kwak
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Young-Ok
YO
Kim
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Dong-Gyun
DG
Kim
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Hee Jeong
HJ
Kong
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Woo-Jin
WJ
Kim
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Jeong-Ha
JH
Kang
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Jung Youn
JY
Park
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Cheul Min
CM
An
Biotechnology Research Division, National Institute of Fisheries Science, Haean-ro 216, Gijang-eup, Gijang-gun, Busan 619-705, Korea.
Ji-Young
JY
Moon
Genetics and Breeding Research Center, National Institute of Fisheries Science, 81-9, Geojenamseo-ro, Dapo-ri, Nambu-myeon, Geoje-si, Gyeongsangnam-do 53334, Republic of Korea.
Choul Ji
CJ
Park
C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Jae Woong
JW
Yu
Interdisciplinary Program in Bioinformatics, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, Republic of Korea.
Joon
J
Yoon
C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Minseok
M
Seo
Interdisciplinary Program in Bioinformatics, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, Republic of Korea.|C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Kwondo
K
Kim
C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Duk Kyung
DK
Kim
C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
SaetByeol
S
Lee
C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Samsun
S
Sung
Interdisciplinary Program in Bioinformatics, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, Republic of Korea.|C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Chul
C
Lee
Research and Development Center, Insilicogen Inc., 13, Heungdeok 1-ro, Giheung-gu, Yongin-si , Gyeonggi-do 16954, Republic of Korea.
Younhee
Y
Shin
Research and Development Center, Insilicogen Inc., 13, Heungdeok 1-ro, Giheung-gu, Yongin-si , Gyeonggi-do 16954, Republic of Korea.
Myunghee
M
Jung
Research and Development Center, Insilicogen Inc., 13, Heungdeok 1-ro, Giheung-gu, Yongin-si , Gyeonggi-do 16954, Republic of Korea.
Byeong-Chul
BC
Kang
Research and Development Center, Insilicogen Inc., 13, Heungdeok 1-ro, Giheung-gu, Yongin-si , Gyeonggi-do 16954, Republic of Korea.
Ga-Hee
GH
Shin
Animal Science and Biotechnology, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, Republic of Korea.
Sojeong
S
Ka
Animal Science and Biotechnology, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-747, Republic of Korea.
Kelsey
K
Caetano-Anolles
C&K Genomics, Main Bldg. #420, SNU Research Park, Gwanak-ro 1, Gwanak-gu, Seoul 151-919, Republic of Korea.
Seoae
S
Cho
Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-921, Republic of Korea.
Heebal
H
Kim
D000818:Animals; D001483:Base Sequence; D049851:Gastropoda / Q000235:genetics*; D016678:Genome; D020539:Sequence Analysis, Protein
Abalone genome; Haliotis discus hannai; Halotidae