Identification, Isolation, and Phylogenetic Analysis of Clostridium perfringens Type A and Type C from Wild Boar ( Sus scrofa ) in the People's Republic of China.
Clostridium perfringens is a Gram-positive, anaerobic, spore-forming bacterium that can induces gas gangrene or enteritis in poultry and humans and many other mammalian species. Here, we report an outbreak of C. perfringens type A and type C coinfection in wild boars ( Sus scrofa ). In February 2016, 10 dead wild boars, including two fresh carcasses, were found in Zhaosu County, Xinjiang Province, People's Republic of China. The two fresh carcasses were included in this study. Two strains of C. perfringens were isolated, identified, genotyped, and phylogenetically analyzed. Based on postmortem examination, bacterium isolation and identification, enterotoxin detection, and auxiliary tests, we made a diagnosis that the wild boar were killed by C. perfringens . Our findings provide the evidence that wild boar can be killed by C. perfringens intoxication. Wild boars are important reservoirs for many zoonotic agents. Therefore, more actions should be taken on the surveillance, prevention, and control of wild pig-borne diseases.
1 National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichenxi Road, Chaoyang District, Beijing, People's Republic of China 100101.|2 University of the Chinese Academy of Sciences, Beijing, People's Republic of China 100047.
Meng
M
Li
1 National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichenxi Road, Chaoyang District, Beijing, People's Republic of China 100101.
Xu
X
Zhang
3 Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, 666 Liuying W Road, Changchun, Jilin Province, People's Republic of China 130122.
Lingwei
L
Zhu
1 National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichenxi Road, Chaoyang District, Beijing, People's Republic of China 100101.
Haifeng
H
Wang
1 National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichenxi Road, Chaoyang District, Beijing, People's Republic of China 100101.|2 University of the Chinese Academy of Sciences, Beijing, People's Republic of China 100047.
Na
N
Zhao
1 National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichenxi Road, Chaoyang District, Beijing, People's Republic of China 100101.
Jing
J
Luo
1 National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichenxi Road, Chaoyang District, Beijing, People's Republic of China 100101.
Chengmin
C
Wang
4 Beijing Animal Husbandry Station, Beijing, People's Republic of China 100000.
Yutian
Y
Wang
4 Beijing Animal Husbandry Station, Beijing, People's Republic of China 100000.
Yanhua
Y
Liu
3 Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, 666 Liuying W Road, Changchun, Jilin Province, People's Republic of China 130122.
Wei
W
Zhou
3 Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, 666 Liuying W Road, Changchun, Jilin Province, People's Republic of China 130122.
Bikai
B
Zhang
3 Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, 666 Liuying W Road, Changchun, Jilin Province, People's Republic of China 130122.
Huancheng
H
Guo
1 National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichenxi Road, Chaoyang District, Beijing, People's Republic of China 100101.
Hongxuan
H
He
D000818:Animals; D002681:China; D003016:Clostridium perfringens / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D034421:Sus scrofa / Q000382:microbiology*; D013552:Swine
Clostridium perfringens; enterotoxin; phylogenetic analysis; wild boar