Nocardia rubra cell-wall skeleton promotes CD4+T cell activation and drives Th1 immune response.
Several lines of evidences have shown that Nocardia rubra cell wall skeleton (Nr-CWS) has immunoregulatory and anti-tumor activities. However, there is no information about the effect of Nr-CWS on CD4+T cells. The aim of this study was to explore the effect of Nr-CWS on the phenotype and function of CD4+T cells. Our results of in vitro experiments showed that Nr-CWS could significantly up-regulate the expression of CD69 and CD25 on CD4+T cells, promote the proliferation of CD4+T cells, increase the production of IFN-γ, TNF-α and IL-2 in the supernatants, but has no significant effect on the apoptosis and death of CD4+T cells. Results of in vivo experiments showed that Nr-CWS could promote the proliferation of CD4+T cells, and increase the production of IL-2, IFN-γ and TNF-α (Th1 type cytokines). These data suggest that Nr-CWS can enhance the activation of CD4+T cells, promote the proliferation of CD4+T cells and the differentiation of CD4+T cells to Th1 cells.
Department of Immunology, School of Basic Medical Science, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Linghe District, Jinzhou 121000, China.
Guangchuan
G
Wang
Department of Oncology, First Affiliated Hospital of Jinzhou Medical University, No. 5-2,Renmin Street, Guta District, Jinzhou, 121001, China.
Jie
J
Wu
Department of Immunology, School of Basic Medical Science, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Linghe District, Jinzhou 121000, China.
Miao
M
Miao
Greatest Biopharma Limited Company, Shiqiaozi Spring Street, Xihu District, Benxi 117004, China.
Heng
H
Dou
Greatest Biopharma Limited Company, Shiqiaozi Spring Street, Xihu District, Benxi 117004, China.
Ning
N
Nan
Greatest Biopharma Limited Company, Shiqiaozi Spring Street, Xihu District, Benxi 117004, China.
Mingsheng
M
Shi
Department of Immunology, School of Basic Medical Science, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Linghe District, Jinzhou 121000, China. Electronic address: yu_guang@hotmail.com.
Guang
G
Yu
Department of Immunology, School of Basic Medical Science, China Medical University, No.77, Puhe Road, Shenyang North New Area, Shenyang 110122, China. Electronic address: fpshan@cmu.edu.cn.
Fengping
F
Shan
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D017358:Cell Wall Skeleton / Q000494:pharmacology*; D016207:Cytokines / Q000097:blood; D005260:Female; D056747:Immunomodulation / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology*
D017358:Cell Wall Skeleton; D016207:Cytokines; C044553:cell wall skeleton, Nocardia
CD4(+) T cell; Immune cells; Immunoregulation; Nocardia rubra cell-wall skeleton