MicroRNA Mediating Networks in Granulosa Cells Associated with Ovarian Follicular Development.
Ovaries, which provide a place for follicular development and oocyte maturation, are important organs in female mammals. Follicular development is complicated physiological progress mediated by various regulatory factors including microRNAs (miRNAs). To demonstrate the role of miRNAs in follicular development, this study analyzed the expression patterns of miRNAs in granulosa cells through investigating three previous datasets generated by Illumina miRNA deep sequencing. Furthermore, via bioinformatic analyses, we dissected the associated functional networks of the observed significant miRNAs, in terms of interacting with signal pathways and transcription factors. During the growth and selection of dominant follicles, 15 dysregulated miRNAs and 139 associated pathways were screened out. In comparison of different styles of follicles, 7 commonly abundant miRNAs and 195 pathways, as well as 10 differentially expressed miRNAs and 117 pathways in dominant follicles in comparison with subordinate follicles, were collected. Furthermore, SMAD2 was identified as a hub factor in regulating follicular development. The regulation of miR-26a/b onsmad2messenger RNA has been further testified by real time PCR. In conclusion, we established functional networks which play critical roles in follicular development including pivotal miRNAs, pathways, and transcription factors, which contributed to the further investigation about miRNAs associated with mammalian follicular development.
Bioengineering Institute of Chongqing University, Chongqing, China.
Baoyun
B
Zhang
Bioengineering Institute of Chongqing University, Chongqing, China.
Long
L
Chen
Sichuan TQLS Animal Husbandry Science and Technology Co., Ltd., Mianyang, China.
Guangde
G
Feng
Bioengineering Institute of Chongqing University, Chongqing, China.
Wei
W
Xiang
Bioengineering Institute of Chongqing University, Chongqing, China.
Ke
K
Zhang
Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Mingxing
M
Chu
Bioengineering Institute of Chongqing University, Chongqing, China.
Pingqing
0000-0002-7993-4011
P
Wang
D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D019295:Computational Biology; D030762:Estrous Cycle; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000166:cytology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009866:Oogenesis; D006080:Ovarian Follicle / Q000254:growth & development* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
D035683:MicroRNAs; D014157:Transcription Factors