Female Hormone 17β-Estradiol Downregulated MMP-2 Expression and Upregulated A1PI Expression in Human Corneal Stromal Cells.
Collagens are essential for cornea functions. In non-ocular tissues, it has been demonstrated that sex hormones modulate the collagen remodeling. In this study, we investigated whether the primary female hormone 17β-estradiol plays a role in the expressions of matrix metalloproteinases and proteinase inhibitors in cultured human corneal stromal cells. We found that 17β-estradiol treatment significantly reduced the matrix metalloproteinase-2 mRNA in human corneal stromal cells as well as the matrix metalloproteinase-2 proteins, while the matrix metalloproteinase-9 mRNA level was not significantly altered. 17β-estradiol also upregulated the expression of proteinase inhibitor, alpha1-proteinase inhibitor. The expression of transcription factor specificity protein 1 was reduced by 17β-estradiol. Furthermore, 17β-estradiol did not change the viability and apoptosis of the corneal stromal cells. The downregulation of matrix metalloproteinase-2 and upregulation of alpha1-proteinase inhibitor by 17β-estradiol possibly serve as protective factor for the normal tomography in antagonizing the extracellular matrix degeneration in many cornea diseases.
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
Hongbo
H
Yin
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.|The Emergency Center of Ophthalmology, The People's Hospital of Leshan, Leshan, Sichuan, People's Republic of China.
Qi
Q
Wan
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
Yan
Y
Tian
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.|Sichuan Provincial People's Hospital, Chengdu, Sichuan, People's Republic of China.
Bo
B
Zhao
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China. ypd_wch@163.com.
Yingping
Y
Deng
D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology; D015536:Down-Regulation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*; D000515:alpha 1-Antitrypsin / Q000235:genetics / Q000378:metabolism*
D016329:Sp1 Transcription Factor; C108209:SP1 protein, human; D000515:alpha 1-Antitrypsin; D004958:Estradiol; D020778:Matrix Metalloproteinase 2
17β-estrodiol; A1PI; Corneal stromal cell; MMP-2; MMP-9; SP1