Jasmonic and salicylic acids enhanced phytochemical production and biological activities in cell suspension cultures of spine gourd (Momordica dioica Roxb).
In vitro cell suspension culture was established for the production of commercially valuable phytochemicals in Momordica dioica. The influence of elicitors in jasmonic acid (JA) and salicylic acid (SA) increased their effect on phytochemical production and biomass accumulation in M. dioica. The results indicate that compared with non-elicited cultures, JA- and SA-elicited cell suspension cultures had significantly enhanced phenolic, flavonoid, and carotenoid production, as well as antioxidant, antimicrobial, and antiproliferative activities. Furthermore, elicited cultures produced 22 phenolic compounds, such as flavonols, hydroxycinnamic acids, and hydroxybenzoic acids. Greater biomass production, phytochemical accumulation, and biological activity occurred in JA- than in SA-elicited cell cultures. This study is the first to successfully establish M. dioica cell suspension cultures for the production of phenolic compounds and carotenoids, as well as for biomass accumulation.
Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University , Seoul - 143 701 , Republic of Korea.
Ill-Min
IM
Chung
Department of Environmental and Herbal Science, Tamil University , Thanjavur 613005, Tamil Nadu , India.
Kaliyaperumal
K
Rekha
Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University , Seoul - 143 701 , Republic of Korea.
Govindasamy
G
Rajakumar
Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University , Seoul - 143 701 , Republic of Korea.
Muthu
M
Thiruvengadam
D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D018533:Biomass; D002338:Carotenoids / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000378:metabolism; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D019073:HT29 Cells; D006801:Humans; D061986:MCF-7 Cells; D029775:Momordica / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D054883:Oxylipins / Q000494:pharmacology*; D010636:Phenols / Q000378:metabolism; D064209:Phytochemicals / Q000378:metabolism*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D020156:Salicylic Acid / Q000494:pharmacology*; D013997:Time Factors
D000890:Anti-Infective Agents; D000975:Antioxidants; D003517:Cyclopentanes; D005419:Flavonoids; D054883:Oxylipins; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts; D010937:Plant Growth Regulators; D002338:Carotenoids; C011006:jasmonic acid; D020156:Salicylic Acid
Carotenoids; cell suspension culture; jasmonic acid; phenolic compounds; salicylic acid