miR-103 regulates triple negative breast cancer cells migration and invasion through targeting olfactomedin 4.
Our previous study showed olfactomedin 4 (OLFM4) suppressed triple-negative breast cancer cells migration, invasion and metastasis-associated protein MMP 9 expression. OLFM4 was identified as a potential target of miR-103 according to microRNA target databases and published studies. The aim of this study is to validate the relationship between miR-103 and OLFM4, and explore the function and clinical significance of miR-103 in triple-negative breast cancer patients. In our results, miR-103 negatively regulated OLFM4 expression by directly targeting its 3'-UTR. OLFM4 was a functional target of miR-103 to regulate triple-negative breast cancer cells migration, invasion and MMP 9 expression. Moreover, miR-103 overexpression was observed in triple-negative breast cancer tissues and cell lines, and associated with lymph node metastasis, distant metastasis and clinical stage. Univariate and multivariate analyses suggested that miR-103 overexpression was a poor independent prognostic factor for triple-negative breast cancer patients. In conclusion, miR-103 acts as an oncogene miRNA to promote triple-negative breast cancer cells migration and invasion through targeting OLFM4.
Surgery Teaching and Research Section, Clinical Medical School, Jining Medical University, NO. 16 Hehua Road, Jining, Shandong 272067, China.
Bin
B
Xiong
Surgery Teaching and Research Section, Clinical Medical School, Jining Medical University, NO. 16 Hehua Road, Jining, Shandong 272067, China.
Xuefeng
X
Lei
Surgery Teaching and Research Section, Clinical Medical School, Jining Medical University, NO. 16 Hehua Road, Jining, Shandong 272067, China.
Lei
L
Zhang
Academy of Basic Medicine, Jining Medical University, NO. 16 Hehua Road, Jining, Shandong 272067, China. Electronic address: fujia2000@cntv.cn.
Jia
J
Fu
D020413:3' Untranslated Regions / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000473:pathology*
D020413:3' Untranslated Regions; C544749:MIRN103 microRNA, human; D035683:MicroRNAs; C454127:OLFM4 protein, human; D016179:Granulocyte Colony-Stimulating Factor; D020780:Matrix Metalloproteinase 9
Biomarker; Breast cancer; Metastasis; OLFM4; miR-103; mircroRNA