Highly Modular Bioluminescent Sensors for Small Molecules and Proteins.
Obtaining patient-specific information through the quantification of small molecules and proteins in bodily fluids is essential for personalized therapies. Point-of-care (POC) diagnostic devices hold the promise of delivering such benefit to a wide range of patients. However, there is a lack of enabling technology, as the majority of newly developed POC devices focus on the same underlying core technologies. Here we provide an overview of a new technology based on highly modular bioluminescent sensors that enables the quantification of small molecules and proteins at the POC with low-cost devices.
École Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Lausanne, Switzerland; École Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Lausanne, Switzerland; National Centre of Competence in Research (NCCR) Chemical Biology, Lausanne, Switzerland.
Qiuliyang
Q
Yu
École Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Lausanne, Switzerland; École Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Lausanne, Switzerland; National Centre of Competence in Research (NCCR) Chemical Biology, Lausanne, Switzerland.
Rudolf
R
Griss
Lucentix SA, Lausanne, Switzerland.
Alberto
A
Schena
École Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Lausanne, Switzerland; École Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Lausanne, Switzerland; National Centre of Competence in Research (NCCR) Chemical Biology, Lausanne, Switzerland. Electronic address: kai.johnsson@epfl.ch.
Kai
K
Johnsson
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008958:Models, Molecular; D004364:Pharmaceutical Preparations / Q000032:analysis*; D019095:Point-of-Care Systems; D011506:Proteins / Q000032:analysis*
D004364:Pharmaceutical Preparations; D011506:Proteins
Bioluminescence; Biosensors; Point-of-care device; Protein engineering