A study on the in vitro percutaneous absorption of silver nanoparticles in combination with aluminum chloride, methyl paraben or di-n-butyl phthalate.
Some reports indicate that the silver released from dermally applied products containing silver nanoparticles (AgNP) (e.g. wound dressings or cosmetics) can penetrate the skin, particularly if damaged. AgNP were also shown to have cytotoxic and genotoxic activity. In the present study percutaneous absorption of AgNP of two different nominal sizes (Ag15nm or Ag45nm by STEM) and surface modification, i.e. citrate or PEG stabilized nanoparticles, in combination with cosmetic ingredients, i.e. aluminum chloride (AlCl3), methyl paraben (MPB), or di-n-butyl phthalate (DBPH) was assessed using in vitro model based on dermatomed pig skin. The inductively coupled plasma mass spectrometry (ICP-MS) measurements after 24h in receptor fluid indicated low, but detectable silver absorption and no statistically significant differences in the penetration between the 4 types of AgNP studied at 47, 470 or 750μg/ml. Similarly, no significant differences were observed for silver penetration when the AgNP were used in combinations with AlCl3(500μM), MPB (1250μM) or DBPH (35μM). The measured highest amount of Ag that penetrated was 0.45ng/cm2(0.365-0.974ng/cm2) for PEG stabilized Ag15nm+MPB.
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland.
Katarzyna
K
Domeradzka-Gajda
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland.
Marek
M
Nocuń
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland.
Joanna
J
Roszak
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland.
Beata
B
Janasik
Applied Spectra, Inc., Fremont, CA, USA.
C Derrick
CD
Quarles
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland.
Wojciech
W
Wąsowicz
Department of Materials Technology and Chemistry, University of Łódź, Łódź, Poland.
Jarosław
J
Grobelny
Department of Materials Technology and Chemistry, University of Łódź, Łódź, Poland.
Emilia
E
Tomaszewska
Department of Materials Technology and Chemistry, University of Łódź, Łódź, Poland.
Grzegorz
G
Celichowski
Department of Materials Technology and Chemistry, University of Łódź, Łódź, Poland.
Katarzyna
K
Ranoszek-Soliwoda
Scientific Department of Unconventional Technologies and Textiles, Textile Research Institute, Łódź, Poland.
Małgorzata
M
Cieślak
Scientific Department of Unconventional Technologies and Textiles, Textile Research Institute, Łódź, Poland.
Dorota
D
Puchowicz
Applied Spectra, Inc., Fremont, CA, USA.
Jhanis J
JJ
Gonzalez
Applied Spectra, Inc., Fremont, CA, USA.
Richard E
RE
Russo
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland. Electronic address: mstep@imp.lodz.pl.
Maciej
M
Stępnik
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002712:Chlorides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D003358:Cosmetics / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D003993:Dibutyl Phthalate / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010226:Parabens / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D010316:Particle Size; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects*; D013499:Surface Properties; D013552:Swine
D017607:Aluminum Compounds; D002712:Chlorides; D003358:Cosmetics; D010226:Parabens; D003993:Dibutyl Phthalate; D000077410:Aluminum Chloride; D012834:Silver; C015358:methylparaben
Aluminum; Di-n-butyl phthalate; Laser ablation ICP-MS; Methyl paraben; Percutaneous absorption; Silver nanoparticles