Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells.
Fusariumspecies are causative agents of human respiratory disorders and are distributed widely in our environment. Little is known of their interaction with human respiratory epithelial cells, which may contribute to allergic airway responses. In this study, we report on the release of C-X-C motif chemokine ligand 8 (CXCL-8) from human bronchial epithelial BEAS-2B cells upon stimulation withFusarium proliferatumextracts.F. proliferatum-induced cytokine release from BEAS-2B cells was determined by cytokine array and CXCL-8 enzyme-linked immunosorbent assay (ELISA) kits. Blocking antibodies and signaling pathway inhibitors were employed to delineate cell surface receptors and signaling pathways participating in CXCL-8 release.F. proliferatumextracts induced the release of CXCL-8 in a time-dependent manner. The dectin-1 receptor ligands, curdlan and laminarin, reduced CXCL-8 release. Cells pre-treated with anti-Dectin-1 antibodies (2 µg/mL) decreased CXCL-8 release by 24%. Furthermore,F. proliferatum-stimulated CXCL-8 release was reduced by 32%, 53%-81%, 40% and 26% after BEAS-2B cells were pretreated with activation inhibitors of spleen tyrosine kinase (Syk)-piceatannol-, mitogen-activated protein kinases (MAPKs)-PD98059, U0126, SB202190, SP600125-, phosphatidylinositol-3-kinase (PI3K)-LY294002-and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-BAY117082-, respectively. These results suggest that Dectin-1-mediated activation of the Syk, MAPKs, PI3K and NF-κB signaling pathways contributes toF. proliferatum-stimulated CXCL-8 release from BEAS-2B cells and provides an important basis for developing novel therapeutic strategies in clinical allergy.
Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei 112, Taiwan. ccyeh39@gmail.com.|Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan. ccyeh39@gmail.com.|Department of Obstetrics and Gynecology, National Yang-Ming University, Taipei 112, Taiwan. ccyeh39@gmail.com.
Chang-Ching
CC
Yeh
Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei 112, Taiwan. hchorng@vghtpe.gov.tw.|Department of Obstetrics and Gynecology, National Yang-Ming University, Taipei 112, Taiwan. hchorng@vghtpe.gov.tw.|Institute of BioMedical Informatics, National Yang-Ming University, Taipei 112, Taiwan. hchorng@vghtpe.gov.tw.
Huann-Cheng
HC
Horng
Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan. chou125@yahoo.com.tw.
Hong
H
Chou
Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan. hytai@vghtpe.gov.tw.
Hsiao-Yun
HY
Tai
Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan. hdshen@vghtpe.gov.tw.
Horng-Der
HD
Shen
Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan. slhsieh@gate.sinica.edu.tw.|Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan. slhsieh@gate.sinica.edu.tw.|Genomics Research Center, Academia Sinica, Taipei 11529, Taiwan. slhsieh@gate.sinica.edu.tw.
Shie-Liang
SL
Hsieh
Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei 112, Taiwan. phwang@vghtpe.gov.tw.|Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan. phwang@vghtpe.gov.tw.|Department of Obstetrics and Gynecology, National Yang-Ming University, Taipei 112, Taiwan. phwang@vghtpe.gov.tw.|Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan. phwang@vghtpe.gov.tw.
Peng-Hui
PH
Wang
D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D060585:Fusariosis / Q000378:metabolism* / Q000382:microbiology; D005670:Fusarium / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000382:microbiology*; D015398:Signal Transduction; D000072377:Syk Kinase / Q000378:metabolism; D047071:beta-Glucans; D000099181:Dectin-1
D016207:Cytokines; D016209:Interleukin-8; D037181:Lectins, C-Type; D020928:Mitogen-Activated Protein Kinases; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; D000072377:Syk Kinase; D047071:beta-Glucans; D000099181:Dectin-1
CXCL-8; Dectin-1; Fusarium proliferatum; respiratory epithelial cells