Effect of monomer blend and chlorhexidine-adding on physical, mechanical and biological properties of experimental infiltrants.
The aim of the study was to evaluate antimicrobial inhibition zone, degree of conversion (DC) and Knoop hardness (KH) of experimental infiltrants. Experimental low viscosity monomer blends were prepared and chlorhexidine diacetate salt (CHX) (0.1% or 0.2%) was added comprising the groups: G1) TEGDMA; G2) TEGDMA/0.1CHX; G3) TEGDMA/0.2CHX; G4) TEGDMA/UDMA; G5) TEGDMA/UDMA/0.1CHX; G6) TEGDMA/UDMA/0.2CHX; G7) TEGDMA/BISEMA; G8) TEGDMA/BISEMA/0.1CHX; G9) TEGDMA/BISEMA/0.2CHX. Icon®was used as control group.



Specimens of resin blends were made (n=9) to accomplished DC and KH. Pour plate was accomplished to evaluate antimicrobial groups' activity against Streptococcus mutans (SM) and Lactobacillus acidophilus (LA). Data obtained were submitted to two-way ANOVA and Tukey tests for blends comparisons and Dunnett's test for comparisons between experimental infiltrants and Icon®(p<0.05).



In relation to antimicrobial effect, uncured blends showed higher antibacterial activity than cured ones for the most of blends. After polymerization, G5 showed the highest inhibition zone against SM and, G3 and G6 against LA. Concerning KH, TEGDMA/UDMA-based blends showed the highest values of KH number and it was influenced by monomeric base, regardless CHX concentration. DC was not affected by monomer blend composition, neither for CHX concentration. The antimicrobial activity was affected by monomeric base, CHX concentration and polymerization. Experimental infiltrants presented similar or higher performance than Icon®for the properties evaluated.



White spot lesion infiltration with low viscosity monomer blends (infiltrants) is an alternative to stop initial caries lesions progression. The incorporation of an antimicrobial agent as chlorhexidine diacetate salt in infiltrants composition could enhance the performance of these materials.
Piracicaba Dental School, Department of Pediatric Dentistry, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: luciana.inagaki@gmail.com.
Luciana Tiemi
LT
Inagaki
Anhanguera University of São Paulo, Biomaterials Department, Rua Maria Cândida, 1813, Bloco G - 6° andar, São Paulo 02071-013, SP, Brazil. Electronic address: robalonso@yahoo.com.
Roberta Caroline Bruschi
RC
Alonso
Piracicaba Dental School, Department of Restorative Dentistry, Dental Materials Division, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: giovanaaraujo@hotmail.com.
Giovana Albamonte Spagnolo
GA
Araújo
University Center of Várzea Grande, Prosthodontic and Dental Materials Department, Várzea Grande, Mato Grosso, Brazil Av Dom Orlando Chaves, 2655, Bairro Cristo Rei - Varzea Grande, 78118-900, MT, Brazil. Electronic address: edujcsj@gmail.com.
Eduardo José Carvalho
EJ
de Souza-Junior
Piracicaba Dental School, Department of Oral Diagnostic, Laboratory of Microbiology and Immunology, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: anibal.paula_c@yahoo.com.br.
Paula Cristina
PC
Anibal
Piracicaba Dental School, Department of Oral Diagnostic, Laboratory of Microbiology and Immunology, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: hofling@fop.unicamp.br.
José Francisco
JF
Höfling
Piracicaba Dental School, Department of Pediatric Dentistry, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: fmpascon@fop.unicamp.br.
Fernanda Miori
FM
Pascon
Piracicaba Dental School, Department of Pediatric Dentistry, P.O. BOX 52, Piracicaba 13414-903, SP, Brazil. Electronic address: rmpuppin@fop.unicamp.br.
Regina Maria
RM
Puppin-Rontani
D002710:Chlorhexidine; D003188:Composite Resins; D006244:Hardness; D008422:Materials Testing; D058105:Polymerization
D003188:Composite Resins; D002710:Chlorhexidine
Antimicrobial activity; BISEMA; Chlorhexidine; Degree of conversion; Knoop hardness; TEGDMA; UDMA