Nonspecific protein adsorption on cationically modified Lyocell fibers monitored by zeta potential measurements.
Nonspecific protein deposition on Lyocell fibers via a cationization step was explored by adsorption of two different N,N,N-trimethyl chitosan chlorides (TMCs). Both, the cationization and the subsequent protein deposition steps were performed and monitored in situ by evaluating the zeta potential using the streaming potential method. Both employed TMCs (degree of substitution with N+Me3Cl groups: 0.27 and 0.64) irreversibly adsorb on the fibers as proven by charge reversal (-12 to +7mV for both derivatives) after the final rinsing step. Onto these cationized fibers, BSA was deposited at different pH values (4, 5, and 7). Charge titrations revealed that close to the isoelectric point of BSA (4.7), BSA deposition was particularly favored, while at lower pH values (pH 4), hardly any adsorption took place due to electrostatic repulsion of the cationic fibers and the positively charged BSA. This work sets the foundation for further investigations to use zeta potential measurements for protein adsorption studies on fibrous materials.
Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria; CD-Laboratory for Fiber Swelling and Paper Performance, Inffeldgasse 23(A), 8010 Graz, Austria.
Claudia
C
Payerl
Laboratory for Characterization and Processing of Polymers (LCPP), University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
Matej
M
Bračič
Graz Centre of Electron Microscopy, and Institute of Electron Microscopy, Graz University of Technology, Steyrergasse 17, 8010 Graz, Austria.
Armin
A
Zankel
CD-Laboratory for Fiber Swelling and Paper Performance, Inffeldgasse 23(A), 8010 Graz, Austria; Institute for Paper, Pulp and Fiber Technology, Inffeldgasse 23, 8010 Graz, Austria.
Wolfgang J
WJ
Fischer
Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria.
Manuel
M
Kaschowitz
Center for Medical Research, Medical University of Graz, Stiftingtalstraße 24, Graz, Austria.
Eleonore
E
Fröhlich
Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria; Laboratory for Characterization and Processing of Polymers (LCPP), University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
Rupert
R
Kargl
Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria.
Franz
F
Stelzer
Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria; CD-Laboratory for Fiber Swelling and Paper Performance, Inffeldgasse 23(A), 8010 Graz, Austria; Laboratory for Characterization and Processing of Polymers (LCPP), University of Maribor, Smetanova 17, 2000 Maribor, Slovenia; Institute for Paper, Pulp and Fiber Technology, Inffeldgasse 23, 8010 Graz, Austria. Electronic address: stefan.spirk@tugraz.at.
Stefan
S
Spirk
D000327:Adsorption; D048271:Chitosan / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
C118071:N-trimethyl chitosan chloride; D011506:Proteins; D012710:Serum Albumin, Bovine; D048271:Chitosan
Chitosan; Lyocell fibers; Protein adsorption; Tenacity; Zeta potential