HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana.
A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Seok Keun
SK
Cho
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Moon Young
MY
Ryu
Carlsberg Research Laboratory, København V, Denmark.
Christian
C
Poulsen
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Jong Hum
JH
Kim
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Tae Rin
TR
Oh
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Suk Won
SW
Choi
Group of host pathogen interaction, Temasek Life Science Laboratory, 1 Research link, National University of Singapore, Singapore.
Mijung
M
Kim
Institute of Biochemistry, National Chung-Hsing University, Taichung, Taiwan.
Jun-Yi
JY
Yang
Department of Biotechnology, College of Applied Life Science (SARI), Jeju National University, Korea.
Kyung Hwan
KH
Boo
DanStem, University of Copenhagen, Denmark.
Naomi
N
Geshi
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Woo Taek
WT
Kim
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.|Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark.
Seong Wook
SW
Yang
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D004706:Endodeoxyribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004720:Endonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism; D010802:Phylogeny; D000072417:Protein Domains; D016601:RNA-Binding Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; C420966:HYL1 protein, Arabidopsis; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C000606277:SE protein, Arabidopsis; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D004722:Endoribonucleases; C000622639:HIGLE protein, Arabidopsis
miRNA precursor processing; microRNA; microprocessor; structure-specific endonuclease