SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand.
Ethylenediamine-modified β-cyclodextrin (Et-β-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO₂@Ag@Et-β-CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-β-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO₂@Ag@Et-β-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin. In particular, luteolin was detected more strongly in the linear range 10-7to 10-3M than various organic molecules, namely ethylene glycol, β-estradiol, isopropyl alcohol, naphthalene, and toluene. In addition, the SERS signal for luteolin captured by the SiO₂@Ag@Et-β-CD NPs remained even after repeated washing. These results indicated that the SiO₂@Ag@Et-β-CD NPs can be used as a rapid, sensitive, and selective sensor for flavonoids.
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea. jm.choi.25@gmail.com.
Jae Min
JM
Choi
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea. dkhei0525@hanmail.net.
Eunil
E
Hahm
Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, Korea. kyeonghee17@naver.com.
Kyeonghui
K
Park
Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, Korea. darkamir@nate.com.
Daham
D
Jeong
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea. Rho72@snu.ac.kr.
Won-Yeop
WY
Rho
Department of Chemistry Education, Seoul National University, Seoul 08826, Korea. susia45@snu.ac.kr.
Jaehi
J
Kim
Department of Chemistry Education, Seoul National University, Seoul 08826, Korea. jeongdh@snu.ac.kr.
Dae Hong
DH
Jeong
School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea. yslee@snu.ac.kr.
Yoon-Sik
YS
Lee
Department of Physics, Konkuk University, Seoul 05029, Korea. shjhang@konkuk.ac.kr.
Sung Ho
SH
Jhang
Department of Physics, Konkuk University, Seoul 05029, Korea. hjchung@konkuk.ac.kr.
Hyun Jong
HJ
Chung
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea. echo@konkuk.ac.kr.
Eunae
E
Cho
Departments of Bacteriology and Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA. jyu1@wisc.edu.
Jae-Hyuk
JH
Yu
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea. bjun@konkuk.ac.kr.
Bong-Hyun
BH
Jun
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea. shjung@konkuk.ac.kr.|Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, Korea. shjung@konkuk.ac.kr.
Seunho
S
Jung
cyclodextrin; ethylenediamine cyclodextrin; flavonoids; surface-enhanced Raman scattering (SERS)