The long non-coding RNA lncFOXO1 suppresses growth of human breast cancer cells through association with BAP1.
Breast cancer, one of the common cancers of women, is the leading cause of death among women below the age of 50 years in western countries. Long non-coding RNAs (lncRNAs) have been shown to be involved in diverse biological processes, both physical and pathological. However, to date, only a few lncRNAs have been functionally identified in breast cancer, and the overall pathophysiological contributions of lncRNAs to breast cancer remain largely unknown. In the present study, we identified a novel lncRNA termed lncFOXO1 through microarray screening. lncFOXO1 is significantly decreased in breast cancer tissues and cell lines and downregulation of lncFOXO1 expression associates with poorer overall survival. Functional assays demonstrated its suppressive role in breast cancer in vivo and in vitro. Mechanistically, lncFOXO1 suppressed the growth of breast cancer by increasing FOXO1 transcription. Moreover, we found that lncFOXO1 associated with BRCA-1-associated protein 1 (BAP1) and regulates its binding and the level of mono-ubiquitinated H2A at K119 (ubH2AK119) at FOXO1 promoter.
Department of Gynecology, Cangzhou Central Hospital, Cangzhou, Heibei 061001, P.R. China.
Jie
J
Xi
Department of Gynecology, Cangzhou Central Hospital, Cangzhou, Heibei 061001, P.R. China.
Jing
J
Feng
Department of Gynecology, Cangzhou Central Hospital, Cangzhou, Heibei 061001, P.R. China.
Qian
Q
Li
Department of Gynecology, Cangzhou Central Hospital, Cangzhou, Heibei 061001, P.R. China.
Xia
X
Li
Department of Gynecology, Cangzhou Central Hospital, Cangzhou, Heibei 061001, P.R. China.
Saitian
S
Zeng
D001943:Breast Neoplasms / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D000071161:Forkhead Box Protein O1 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D016019:Survival Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000235:genetics* / Q000378:metabolism
C400134:BAP1 protein, human; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D062085:RNA, Long Noncoding; D025521:Tumor Suppressor Proteins; C000619242:long non-coding RNA RHOXF1, human; D043222:Ubiquitin Thiolesterase