[Biomineralization of electrospun polycaprolactone-guided bone regeneration membrane].
OBJECTIVE
To evaluate the biomineralization of the tissue-engineering electrospun polycaprolactone (PCL) scaffold and its potential use for guided bone regeneration (GBR) membranes.


METHODS
PCL ultrafinefiber scaffolds were fabricated by electrospinning and then immersed in supersaturated calcification solution (SCS) for biomineralization investigation. The electrospun PCL scaffolds and the calcium phosphate coating were identified by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Water-contact angles were also measured to evaluate the hydrophilicity of the modified surface. The biocompatibility of the composite was investigated by culturing osteoblasts on the scaffolds. Cell behavior was observed by SEM.


RESULTS
The electrospun PCL scaffold was composed of ultrafine fibers and well-interconnected pores. The deposits on the fibers grew in number and size as the biomineralization time increased. Then, the covering of the whole PCL film was identified as dicalcium phosphate dehydrate and apatite. Good cell attachment and proliferation behavior were observed on the membranes.


CONCLUSIONS
The quick apatite formation on the surface of the PCL electrospun scaffold indicated that SCS biomineralization may be an effective approach for obtaining PCL/calcium phosphate composites. The cellular biocompatibility of the composite scaffold was preliminarily confirmed by the in vitro culture of osteoblasts on the scaffold. As such, the composite scaffold is a promising biomimetic extracellular matrix biomaterial for bone tissue engineering and GBR membranes.
Dept. of Stomatology, Nuclear of Industry 416 Hospital, Chengdu 610051, China.
Deng
D
Xia
Dept. of Implantology, College of Stomatology, Chongqing University of Medical Science, Chongqing 400015, China.
Bai
B
Shi
D001672:Biocompatible Materials; D001861:Bone Regeneration; D002130:Calcium Phosphates; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts; D011091:Polyesters; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; C016240:polycaprolactone; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous
biomineralization; calcium phosphate; electrospun; guided bone regeneration; polycaprolactone