Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells.
The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocyte maturation. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, and GCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term (168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6μM E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1 mRNA was measured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, and CYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Sylwia
S
Ciesiółka
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Joanna
J
Budna
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Karol
K
Jopek
Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Artur
A
Bryja
Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Wiesława
W
Kranc
Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Sylwia
S
Borys
Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Obilni trh 11, 602 00 Brno, Czech Republic.
Michal
M
Jeseta
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Adrian
A
Chachuła
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland; Department of Anatomy and Histology, Faculty of Medicine and Health Sciences, University of Zielona Gora, Ul. Zyty 28, 65-046 Zielona Gora, Poland.
Agnieszka
A
Ziółkowska
Institute of Veterinary and Animal Science, Poznan University of Life Sciences, 52 Wojska Polskiego St., 60-628 Poznań, Poland.
Paweł
P
Antosik
Institute of Veterinary and Animal Science, Poznan University of Life Sciences, 52 Wojska Polskiego St., 60-628 Poznań, Poland.
Dorota
D
Bukowska
Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Klaus P
KP
Brüssow
Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Małgorzata
M
Bruska
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Michał
M
Nowicki
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Maciej
0000-0003-2004-0010
M
Zabel
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland; Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland.
Bartosz
0000-0002-6804-0119
B
Kempisty
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects*; D000072496:Cytochrome P450 Family 19 / Q000096:biosynthesis; D004958:Estradiol / Q000008:administration & dosage; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development; D009866:Oogenesis / Q000187:drug effects* / Q000235:genetics; D011962:Receptors, FSH / Q000096:biosynthesis / Q000235:genetics; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics; D013552:Swine
D011962:Receptors, FSH; D011974:Receptors, LH; D004958:Estradiol; D000072496:Cytochrome P450 Family 19