Metabolic and cellular stress responses of catfish, Horabagrus brachysoma (Günther) acclimated to increasing temperatures.
We investigated the metabolic and cellular stress responses in an endemic catfish Horabagrus brachysoma acclimated to ambient (26°C), 31, 33 and 36°C for 30 days. After acclimation, fish were sampled to investigate changes in the levels of blood glucose, tissue glycogen and ascorbic acid, activities of enzymes involved in glycolysis (LDH), citric acid cycle (MDH), gluconeogenesis (FBPase and G6Pase), pentose phosphate pathway (G6PDH), protein metabolism (AST and ALT), phosphate metabolism (ACP and ALP) and energy metabolism (ATPase), and HSP70 levels in various tissues. Acclimation to higher temperatures (33 and 36°C) significantly increased activities of LDH, MDH, ALP, ACP, AST, ALT and ATPase and blood glucose levels, whereas decreased the G6PDH enzyme activity and, tissue glycogen and ascorbic acid. Results indicated an overall increase in the carbohydrate, protein and lipid metabolism implying increased metabolic demands for maintaining homeostasis in fish acclimated to higher temperatures (33 and 36°C). We observed tissue specific response of HSP70 in H. brachysoma, with significant increase in gill and liver at 33 and 36°C, and in brain and muscle at 36°C, enabling cellular protection at higher acclimation temperatures. In conclusion, H. brachysoma adjusted metabolic and cellular responses to withstand increased temperatures, however, these responses suggest that the fish was under stress at 33°C or higher temperature.
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India; Department of Zoology, Maharshi Dayanand College, Parel, Mumbai 400012, India. Electronic address: rishi.dalvi@gmail.com.
Rishikesh S
RS
Dalvi
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India; Department of Zoology, Raja N. L. Khan Womens' College, Paschim Medinipur 721102, West Bengal, India.
Tilak
T
Das
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India; ICAR-Central Inland Fisheries Research Institute, Regional Centre, HOUSEFED Complex, Dispur, Guwahati 781 006, Assam, India.
Dipesh
D
Debnath
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India; ICAR-Central Inland Fisheries Research Institute, Regional Centre, HOUSEFED Complex, Dispur, Guwahati 781 006, Assam, India.
Sona
S
Yengkokpam
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India; Lab of Aquaculture and Artemia Reference Centre, Ghent University, Rozier 44, 9000 Ghent, Belgium.
Kartik
K
Baruah
Department of Zoology, Maharshi Dayanand College, Parel, Mumbai 400012, India.
Lalchand R
LR
Tiwari
Division of Fish Nutrition and Biochemistry, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Asim K
AK
Pal
D000064:Acclimatization; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002397:Catfishes / Q000097:blood / Q000502:physiology*; D004734:Energy Metabolism; D029941:Fish Proteins / Q000032:analysis / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism; D006358:Hot Temperature; D050356:Lipid Metabolism; D053858:Metabolic Networks and Pathways; D013312:Stress, Physiological
D001786:Blood Glucose; D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins
HSP70; Horabagrus brachysoma; Metabolic enzymes; Secondary stress responses; Thermal acclimation