RNA-Guided CRISPR-Cas9 System-Mediated Engineering of Acute Myeloid Leukemia Mutations.
Current acute myeloid leukemia (AML) disease models face severe limitations because most of them induce un-physiological gene expressions that do not represent conditions in AML patients and/or depend on external promoters for regulation of gene expression/repression. Furthermore, many AML models are based on reciprocal chromosomal translocations that only reflect the minority of AML patients, whereas more than 50% of patients have a normal karyotype. The majority of AML, however, is driven by somatic mutations. Thus, identification as well as a detailed molecular and functional characterization of the role of these driver mutations via improved AML models is required for better approaches toward novel targeted therapies. Using the IDH2 R140Q mutation as a model, we present a new effective methodology here using the RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system to reproduce or remove AML-associated mutations in or from human leukemic cells, respectively, via introduction of a DNA template at the endogenous gene locus via homologous recombination. Our technology represents a precise way for AML modeling to gain insights into AML development and progression and provides a basis for future therapeutic approaches.
Department of Medicine A, University of Muenster, 48149 Muenster, Germany.
Oliver
O
Brabetz
Department of Medicine A, University of Muenster, 48149 Muenster, Germany.
Vijay
V
Alla
Department of Medicine A, University of Muenster, 48149 Muenster, Germany.
Linus
L
Angenendt
Department of Medicine A, University of Muenster, 48149 Muenster, Germany.
Christoph
C
Schliemann
Department of Medicine A, University of Muenster, 48149 Muenster, Germany.
Wolfgang E
WE
Berdel
Department of Medicine A, University of Muenster, 48149 Muenster, Germany. Electronic address: marifrancis.arteaga@uni-muenster.de.
Maria-Francisca
MF
Arteaga
Department of Medicine A, University of Muenster, 48149 Muenster, Germany. Electronic address: jan-henrik.mikesch@ukmuenster.de.
Jan-Henrik
JH
Mikesch
AML; CRISPR-Cas9; genome editing; leukemia