Vapor Phase Exchange of Self-Assembled Monolayers for Engineering of Biofunctional Surfaces.
We show that 4'-nitro-1,1'-biphenyl-4-thiol self-assembled monolayers (NBPT SAMs) on gold can be exchanged with 11-(mercaptoundecyl)triethylene glycol (C11EG3OH) SAMs via vapor deposition (VD). The pristine and the exchanged SAMs obtained by VD as well as solution method (SM) were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). Using surface plasmon resonance (SPR), it is shown that C11EG3OH SAMs on gold obtained by vapor deposition exchange (VDEx) have the same protein resistivity as SAMs obtained by the direct self-assembly process. As expected, the cross-linked NBPT SAM are found to be resistive to both exchange processes, VDExand solution method exchange (SMEx). In this way, VDExopens up an elegant and new approach of patterning SAM surfaces in situ at vacuum conditions without using any solvents. By combining electron irradiation-induced chemical lithography of NBPT SAMs with VDEx, biofunctional patterned substrates were engineered and used for immobilization of protein arrays.
Department of Physics, Physics of Supramolecular Systems and Surfaces, University of Bielefeld , 33615 Bielefeld, Germany.|Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, 14195 Berlin, Germany.
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0000-0002-6624-8012
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Kankate
Department of Physics, Physics of Supramolecular Systems and Surfaces, University of Bielefeld , 33615 Bielefeld, Germany.
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Aguf
Institute of Biochemistry, Biocenter, Goethe-University , 60438 Frankfurt, Germany.
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Großmann
Department of Physics, Physics of Supramolecular Systems and Surfaces, University of Bielefeld , 33615 Bielefeld, Germany.
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Schnietz
Institute of Biochemistry, Biocenter, Goethe-University , 60438 Frankfurt, Germany.
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0000-0002-0403-2160
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Tampé
Institute of Physical Chemistry, Friedrich Schiller University Jena , Lessingstr. 10, 07743 Jena, Germany.
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Turchanin
Department of Physics, Physics of Supramolecular Systems and Surfaces, University of Bielefeld , 33615 Bielefeld, Germany.
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Gölzhäuser
D006046:Gold; D056951:Photoelectron Spectroscopy; D013438:Sulfhydryl Compounds; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D014835:Volatilization
D013438:Sulfhydryl Compounds; D006046:Gold