Cytotoxicity of GuttaFlow Bioseal, GuttaFlow2, MTA Fillapex, and AH Plus on Human Periodontal Ligament Stem Cells.
BACKGROUND
The aim of the present study was to evaluate the in vitro cytotoxicity of endodontic sealers (GuttaFlow Bioseal, GuttaFlow2, and MTA Fillapex) on human periodontal ligament stem cells (hPDLSCs). As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability and cell attachment.


METHODS
Biological testing was carried out in vitro on hPDLSCs. Cell viability assay was performed by using eluates from each endodontic sealer. To assess cell morphology and attachment to the different sealers, the hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. Chemical composition of the sealers was determined by energy-dispersive x-ray, and eluates were analyzed by inductively coupled plasma mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05).


RESULTS
Cell viability was evident after 24 hours in the presence of GuttaFlow Bioseal and GuttaFlow 2 but not in the case of AH Plus or MTA Fillapex. At 168 hours, GuttaFlow Bioseal and GuttaFlow 2 exhibited high and moderate cell viability, respectively, whereas AH Plus and MTA Fillapex revealed low rates of cell cell viability (P < .001). Finally, scanning electron microscopy studies revealed a high degree of proliferation, cell spreading, and attachment, especially when using GuttaFlow Bioseal disks.


CONCLUSIONS
GuttaFlow Bioseal and GuttaFlow2 showed lower cytotoxicity than MTA Fillapex and AH plus. Further in vitro and in vivo investigations are required to confirm the suitability of GuttaFlow Bioseal for clinical application.
Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
Mar
M
Collado-González
Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain; School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain.
Christopher J
CJ
Tomás-Catalá
School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain.
Ricardo E
RE
Oñate-Sánchez
Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
José M
JM
Moraleda
Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain; School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain. Electronic address: fcojavier@um.es.
Francisco J
FJ
Rodríguez-Lozano
D017607:Aluminum Compounds / Q000009:adverse effects*; D017610:Calcium Compounds / Q000009:adverse effects*; D002470:Cell Survival / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000009:adverse effects*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000009:adverse effects*; D006171:Gutta-Percha / Q000009:adverse effects*; D006801:Humans; D010087:Oxides / Q000009:adverse effects*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000009:adverse effects*; D017640:Silicates / Q000009:adverse effects*; D013234:Stem Cells / Q000187:drug effects*
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D004853:Epoxy Resins; C515718:GuttaFlow; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C534916:epoxy resin-based root canal sealer; C086631:mineral trioxide aggregate; D006171:Gutta-Percha
Adhesion; calcium silicate-based endodontic sealers; cytotoxicity; human periodontal ligament stem cells; mineral trioxide aggregate