In ovovaccination usingEimeriaprofilin andClostridium perfringensNetB proteins in Montanide IMS adjuvant increases protective immunity against experimentally-induced necrotic enteritis.
OBJECTIVE
The effects of vaccinating 18-day-old chicken embryos with the combination of recombinantEimeriaprofilin plusClostridium perfringens(C. perfringens) NetB proteins mixed in the Montanide IMS adjuvant on the chicken immune response to necrotic enteritis (NE) were investigated using anEimeria maxima(E. maxima)/C. perfringensco-infection NE disease model that we previously developed.


METHODS
Eighteen-day-old broiler embryos were injected with 100 μL of phosphate-buffered saline, profilin, profilin plus necrotic enteritis B-like (NetB), profilin plus NetB/Montanide adjuvant (IMS 106), and profilin plus Net-B/Montanide adjuvant (IMS 101). After post-hatch birds were challenged with our NE experimental disease model, body weights, intestinal lesions, serum antibody levels to NetB, and proinflammatory cytokine and chemokine mRNA levels in intestinal intraepithelial lymphocytes were measured.


RESULTS
Chickensin ovovaccinated with recombinant profilin plus NetB proteins/IMS106 and recombinant profilin plus NetB proteins/IMS101 showed significantly increased body weight gains and reduced gut damages compared with the profilin-only group, respectively. Greater antibody response to NetB toxin were observed in the profilin plus NetB/IMS 106, and profilin plus NetB/IMS 101 groups compared with the other three vaccine/adjuvant groups. Finally, diminished levels of transcripts encoding for proinflammatory cytokines such as lipopolysaccharide-induced tumor necrosis factor-α factor, tumor necrosis factor superfamily 15, and interleukin-8 were observed in the intestinal lymphocytes of chickensin ovoinjected with profilin plus NetB toxin in combination with IMS 106, and profilin plus NetB toxin in combination with IMS 101 compared with profilin protein alone bird.


CONCLUSIONS
These results suggest that the Montanide IMS adjuvants potentiate host immunity to experimentally-induced avian NE when administeredin ovoin conjunction with the profilin and NetB proteins, and may reduce disease pathology by attenuating the expression of proinflammatory cytokines and chemokines implicated in disease pathogenesis.
Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Hyun Soon
HS
Lillehoj
Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.|Institute of Health and Environment, Daejeon Metropolitan City, Daejeon 305-338, Korea.
Seung Ik
SI
Jang
Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Alfredo
A
Panebra
Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Erik Peter
EP
Lillehoj
Seppic, 22 Terrasse Bellini, Puteaux 92800, France.
Laurent
L
Dupuis
Seppic, 22 Terrasse Bellini, Puteaux 92800, France.
Juliette
J
Ben Arous
Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Seung Kyoo
SK
Lee
Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Sung Taek
ST
Oh
Clostridium perfringens; Eimeria; Immunity; In ovo Vaccination; Necrotic Enteritis; Profilin