Cell and region specificity of Aryl hydrocarbon Receptor (AhR) system in the testis and the epididymis.
Aryl hydrocarbon receptor (AhR) plays multiple important functions in adaptive responses. Exposure to AhR ligands may produce an altered metabolic activity controlled by the AhR pathways, and consequently affect drug/toxin responses, hormonal status and cellular homeostasis. This research revealed species-, cell- and region-specific pattern of the AhR system expression in the rat and human testis and epididymis, complementing the existing knowledge, especially within the epididymal segments. The study showed that AhR level in the rat and human epididymis is higher than in the testis. The downregulation of AhR expression after TCDD treatment was revealed in the spermatogenic cells at different stages and the epididymal epithelial cells, but not in the Sertoli and Leydig cells. Hence, this basic research provides information about the AhR function in the testis and epididymis, which may provide an insight into deleterious effects of drugs, hormones and environmental pollutants on male fertility.
Department of Experimental and Clinical Pharmacology, Pomeranian Medical University, Szczecin, Poland. Electronic address: annawajda2046@gmail.com.
A
A
Wajda
Department of Experimental and Clinical Pharmacology, Pomeranian Medical University, Szczecin, Poland.
J
J
Łapczuk
Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland.
M
M
Grabowska
Department of General Pathology, Pomeranian Medical University, Poland.
E
E
Pius-Sadowska
Department of Urology and Urological Oncology, Pomeranian Medical University, Szczecin, Poland.
M
M
Słojewski
Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland.
M
M
Laszczynska
Department of Pathology, Pomeranian Medical University, Szczecin, Poland.
E
E
Urasinska
Department of General Pathology, Pomeranian Medical University, Poland.
B
B
Machalinski
Department of Experimental and Clinical Pharmacology, Pomeranian Medical University, Szczecin, Poland.
M
M
Drozdzik
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000368:Aged; D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D065633:Cytochrome P-450 CYP1B1 / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity; D004822:Epididymis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D029362:Organic Anion Transporters, Sodium-Independent / Q000235:genetics; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics; D013737:Testis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000097202:ATP-Binding Cassette Sub-Family B Member 4
D018435:ATP Binding Cassette Transporter, Subfamily B; D018528:ATP-Binding Cassette Transporters; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D051792:Basic Helix-Loop-Helix Proteins; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1; D004785:Environmental Pollutants; D029362:Organic Anion Transporters, Sodium-Independent; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D012097:Repressor Proteins; D000097202:ATP-Binding Cassette Sub-Family B Member 4; C497398:ARNT protein, rat; C423013:Abcc2 protein, rat; C492161:Ahrr protein, rat; C421143:Slco1a5 protein, rat; C585499:Cyp1b1 protein, rat
Aryl hydrocarbon receptor; Epididymis; TCDD; Testis