Versatile synthesis of the signaling peptide glorin.
We present a versatile synthesis of the eukaryotic signaling peptide glorin as well as glorinamide, a synthetic analog. The ability of these compounds to activate glorin-induced genes in the social amoebaPolysphondylium pallidumwas evaluated by quantitative reverse transcription PCR, whereby both compounds showed bioactivity comparable to a glorin standard. This synthetic route will be useful in conducting detailed structure-activity relationship studies as well as in the design of chemical probes to dissect glorin-mediated signaling pathways.
Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute - HKI, Junior Research Group Chemistry of Microbial Communication, Beutenbergstr. 11, D-07745 Jena, Germany.
Robert
R
Barnett
Faculty of Biology and Pharmacy, Institute of Pharmacy, Department of Pharmaceutical Biology, University of Jena, Semmelweisstrasse 10, D-07743 Jena, Germany.
Daniel
D
Raszkowski
Faculty of Biology and Pharmacy, Institute of Pharmacy, Department of Pharmaceutical Biology, University of Jena, Semmelweisstrasse 10, D-07743 Jena, Germany.
Thomas
T
Winckler
Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute - HKI, Junior Research Group Chemistry of Microbial Communication, Beutenbergstr. 11, D-07745 Jena, Germany.
Pierre
P
Stallforth
Dictyostelium; Polysphondylium; glorin; multicellularity; signaling molecules; social amoebae