Benzo(a)pyrene Exposure Causes Genotoxic and Biochemical Changes in the Midge Larvae of Chironomus sancticaroli Strixino & Strixino (Diptera: Chironomidae).
Benzo(a)pyrene (BaP) is a carcinogenic polycyclic aromatic hydrocarbon, also found in nature due to human activities. BaP adheres to sediments showing toxic effects on benthic organisms, including midge larvae of the family Chironomidae. We tested for toxic effects of benzo(a)pyrene on Chironomus sancticaroli Strixino & Strixino 1981 using biochemical and genotoxic biomarkers, to identify changes in metabolic and antioxidant pathways, besides neurotoxic and DNA damage. Enzyme activity was compared by exposing larvae to four nominal concentrations (0.47, 2.13, 3.41, and 4.73 μg l-1) and DNA damage to two concentrations (0.47 and 4.73 μg l-1), after exposure at 24, 48, 72, and 96 h. BaP caused neurotoxic effect, showing acetylcholinesterase alterations at different treatments. Changes in the biotransformation pathway were detected, with an increased activity of alpha and beta esterase in 48 h and reduction of glutathione-S-transferase activity in all periods at the highest concentrations. Damage to the antioxidant system was observed by the increase of the superoxide dismutase and reduction of the catalase, in 48 h. Genotoxicity was detected by an increased DNA damage at 48 and 72 h. The lowest concentration (0.47 μg l-1), even presenting low mortality, also altered the biochemical parameters of the larvae. Thus, these results indicate that BaP causes metabolic, neurotoxic, and genotoxic effects on C. sancticaroli, even at low concentrations and short-term exposure. BaP can cause damage of immature invertebrates, and the ecological dynamics can be affected, since these organisms have trophic importance in the aquatic environment.
Depto de Zoologia, Univ Federal do Paraná, Caixa Postal 19020, 81531-980, Curitiba, Paraná, Brasil.
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Vicentini
Depto de Zoologia, Univ Federal do Paraná, Caixa Postal 19020, 81531-980, Curitiba, Paraná, Brasil.
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Morais
Depto de Zoologia, Univ Federal do Paraná, Caixa Postal 19020, 81531-980, Curitiba, Paraná, Brasil.
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Rebechi-Baggio
Depto de Zoologia, Univ Federal do Paraná, Caixa Postal 19020, 81531-980, Curitiba, Paraná, Brasil.
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Richardi
Depto de Genética, Univ Federal do Paraná, Curitiba, Paraná, Brasil.
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Santos
Depto de Genética, Univ Federal do Paraná, Curitiba, Paraná, Brasil.
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MM
Cestari
Depto de Zoologia, Univ Federal do Paraná, Caixa Postal 19020, 81531-980, Curitiba, Paraná, Brasil. mnavarro@ufpr.br.
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MA
Navarro-Silva
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002683:Chironomidae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
D000975:Antioxidants; D001564:Benzo(a)pyrene; D000110:Acetylcholinesterase
Bioassays; DNA damage; PAH; enzymes; invertebrates