HLA expression and HLA type associations in relation to EBV status in Hispanic Hodgkin lymphoma patients.
A proportion of classical Hodgkin lymphomas harbor the Epstein Barr virus (EBV). We previously demonstrated that associations between Human Leukocyte Antigen (HLA) alleles and susceptibility to EBV+ classical Hodgkin lymphoma differ between European and Chinese populations. Data on Hispanic populations is missing. Here we examined the association between HLA type, tumor cell HLA expression and other characteristics in Hispanic Hodgkin lymphoma patients. Hispanic Hodgkin lymphoma patients diagnosed at the Los Angeles County-University of Southern California Medical Center from 2000-2012 were included (n = 65). Formalin-fixed paraffin-embedded tumor tissue was analyzed for EBV by in situ hybridization and for HLA class I and class II expression by immunohistochemistry. HLA typing was performed by HLA-A specific quantitative PCR of genomic DNA from tissue. Thirty patients (46%) had EBV+ tumors. Expression of HLA class I (p = 0.0006) was significantly associated with EBV+ tumor status in Hispanic patients, similar to Europeans and Chinese. A positive association between HLA class II expression and EBV+ tumor status, as present in large studies in Europeans, was not found (p = 0.06). The prevalences of the specific European HLA-A*01 risk and European HLA-A*02 protective types were not significantly associated with EBV+ tumors among these Hispanic patients, however numbers were too low to draw firm conclusions. The HLA-A*02:07 allele, that is associated with EBV+ Hodgkin lymphoma in Chinese, was absent. In conclusion, the association between EBV positivity in tumor cells and HLA class I expression appears to be consistent across different populations. Larger studies in Hispanics are needed to evaluate HLA allele susceptibility associations.
Department of Preventive Medicine and Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, University of Southern California, Los Angeles, California, United States of America.
Luke B
LB
Fletcher
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
Rianne N
RN
Veenstra
Department of Pathology and Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, University of Southern California, Los Angeles, California, United States of America.
Eric Y
EY
Loo
Department of Preventive Medicine and Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, University of Southern California, Los Angeles, California, United States of America.
Amie E
AE
Hwang
Department of Pathology and Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, University of Southern California, Los Angeles, California, United States of America.
Imran N
IN
Siddiqi
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
Lydia
L
Visser
Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
Bouke G
BG
Hepkema
Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
Ilja M
IM
Nolte
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
Anke
A
van den Berg
Department of Preventive Medicine and Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, University of Southern California, Los Angeles, California, United States of America.|Department of Pathology and Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, University of Southern California, Los Angeles, California, United States of America.
Wendy
W
Cozen
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
Arjan
A
Diepstra
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D020031:Epstein-Barr Virus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D005260:Female; D005787:Gene Frequency; D006680:HLA Antigens / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000235:genetics / Q000276:immunology* / Q000821:virology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D006680:HLA Antigens