Transformation of waste cooking oil into C-18 fatty acids using a novel lipase produced by Penicillium chrysogenum through solid state fermentation.
The prime aim of the current work was to illustrate the components existing in repeatedly used cooking oil and to develop an economical process for the production of fatty acids from low cost feedstock waste. The waste cooking oil was characterized by the occurrence of high molecular weight hydrocarbons and polymerized derivative of esters. Triacontanoic acid methyl ester, 2,3,5,8-Tetramethyldecane, 3,3 dimethyl heptane, and 2,2,3,3-teramethyl pentane were detected as thermal and oxidative contaminants that adversely affect the quality of cooking oil. Fundamentally, waste cooking oil comprises ester bonds of long chain fatty acids. The extracellular lipase produced from P. chrysogenum was explored for the hydrolysis of waste cooking oil. The incorporation of lipase to waste cooking oil in 1:1 proportion released 17 % oleic acid and 5 % stearic acid.
Department of Biotechnology, Motilal Nehru National Institute of Technology, Teliyarganj, Allahabad, 221004, India. sunilbt13@gmail.com.
Sunil
S
Kumar
Department of Biotechnology, Motilal Nehru National Institute of Technology, Teliyarganj, Allahabad, 221004, India.
Sangeeta
S
Negi
Chromatography; Fatty acid; Lipase; Waste cooking oil