Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil.
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from vulvovaginal mucus, but the semen samples were all negative. The isolates were submitted to Pulsed-field gel electrophoresis methodology and their 16S rRNA genes were sequenced. Fifty percent of ureaplasma recovered from sheep allowed for PFGE typing. Eleven isolates showed eight profiles genetically close to the bovine ureaplasmas. The 16S rRNA gene sequencing showed differences or similarities of isolates from sheep and goats, and the reference strains of bovine and human ureaplasma. Four clinical isolates from sheep were grouped separately. The studied ureaplasma isolates showed to be a diverse group of mollicutes.
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
Rosângela C
RC
Oliveira
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
Maurício
M
Yamaguti
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil; Universidade Federal da Bahia, Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Bahia, BA, Brazil. Electronic address: lucasm@usp.br.
Lucas M
LM
Marques
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
Melissa
M
Buzinhani
Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Pualo, SP, Brazil.
Huber
H
Rizzo
Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Pualo, SP, Brazil.
Enoch Brandão de Souza
EBS
Meira-Junior
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
Ana Márcia S
AMS
Guimarães
Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Pualo, SP, Brazil.
Lilian
L
Gregory
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
Jorge
J
Timenetsky
D000818:Animals; D001938:Brazil; D005260:Female; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D008297:Male; D012661:Semen / Q000382:microbiology*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D014509:Ureaplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016869:Ureaplasma Infections / Q000382:microbiology / Q000662:veterinary*; D014621:Vagina / Q000382:microbiology*
Caprine; Molecular characterization; Ovine; Ureaplasma