Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy.
Allergen-specific Immunoglobulin E (IgE) determination lies at the heart of diagnosis of sensitization to food and other allergens. In the past few years, reporter systems capable of detecting the presence of allergen-specific IgE have been developed by several labs. These rely on humanized rat basophil leukemia cell lines stably transfected with reporter genes such as firefly luciferase. In this chapter, we describe protocols for the use of the RS-ATL8 cell line (IgE cross-linking-induced luciferase expression; EXiLE) in 96-well and 384-well formats. We also describe optional treatment steps for enveloped virus and complement inactivation.
Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Boots Science Bldg., Science Road, Nottingham, NG7 2RD, UK.
Eman Ali
EA
Ali
Division of Medicinal Safety Science, National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan.
Ryosuke
R
Nakamura
Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Boots Science Bldg., Science Road, Nottingham, NG7 2RD, UK. franco.falcone@nottingham.ac.uk.
Franco H
FH
Falcone
D000485:Allergens / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008156:Luciferases / Q000276:immunology; D051381:Rats
D000485:Allergens; D007073:Immunoglobulin E; D008156:Luciferases
Allergy; EXiLE; Humanized RBL; IgE; Luciferase; RS-ATL8; Reporter system