Collagen-cellulose nanocrystal scaffolds containing curcumin-loaded microspheres on infected full-thickness burns repair.
Burn infection is a serious problem that delays wound healing and leads to death. Curcumin (Cur) has been shown to exhibit antioxidant, anti-inflammatory, antimicrobial and anticarcinogenic activity. However, its instability, extremely low aqueous solubility and bioavailability in physiological fluids may make it difficult to maintain local Cur concentrations above the minimum inhibitory concentration for burn infection treatment. The objective of this study was to construct complexes of Cur/gelatin microspheres (GMs) and porous collagen (Coll)-cellulose nanocrystals (CNCs) composite scaffolds for full-thickness burn infection treatment. The Cur/GMs/Coll-CNCs scaffolds had high porosity, available pore size, and a long and sustained Cur release profile. Furthermore, the composite scaffold exhibited remarkably strong antibacterial activity. Hence, we evaluated the wound-healing effects and antibacterial properties of Cur/GMs/Coll-CNCs scaffolds in a rat full-thickness burn infection model. The Cur/GMs/Coll-CNCs scaffold was able to prevent not only local inflammation but also accelerated dermis regeneration. Thus, we conclude that Cur/GMs/Coll-CNCs scaffolds can act as an effective dermal regeneration template for full-thickness burn wound infection healing in rats models. Copyright © 2017 John Wiley & Sons, Ltd.
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China.|Department of Biomedical Engineering, Jinan University, Guangzhou, China.
Rui
R
Guo
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China.|Department of Biomedical Engineering, Jinan University, Guangzhou, China.
Yong
Y
Lan
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China.|Department of Biomedical Engineering, Jinan University, Guangzhou, China.
Wei
W
Xue
Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China.
Biao
B
Cheng
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China.|Department of Chemistry, Jinan University, Guangzhou, China.
Yuanming
Y
Zhang
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, China.
Changyong
C
Wang
Guangdong-Hongkong-Macau Institute of CNS Regeneration (GHMICR), Jinan University, Guangzhou, China.
Seeram
S
Ramakrishna
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002056:Burns / Q000188:drug therapy*; D002417:Cattle; D002482:Cellulose / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D065546:Drug Liberation; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008826:Microbial Sensitivity Tests; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects*; D012867:Skin / Q000473:pathology; D054457:Tissue Scaffolds / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
D000900:Anti-Bacterial Agents; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D002482:Cellulose; D003094:Collagen; D003474:Curcumin
curcumin; full-thickness burn; infection; microspheres; scaffolds; wound healing