Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.
Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Yuki
Y
Fujii
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Mika K
MK
Kaneko
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Satoshi
S
Ogasawara
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Shinji
S
Yamada
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Miyuki
M
Yanaka
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Takuro
T
Nakamura
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Noriko
N
Saidoh
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Kanae
K
Yoshida
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
Ryusuke
R
Honma
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .|2 New Industry Creation Hatchery Center, Tohoku University , Sendai, Japan .
Yukinari
Y
Kato
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000915:Antibody Affinity; D000918:Antibody Specificity; D016466:CHO Cells; D003412:Cricetulus; D066246:ErbB Receptors / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015870:Gene Expression; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D000098853:Podoplanin
D000911:Antibodies, Monoclonal; D066246:ErbB Receptors; D008562:Membrane Glycoproteins; D010455:Peptides; C092025:Pdpn protein, rat; D000098853:Podoplanin
RAP tag; affinity tag; monoclonal antibody; podoplanin; protein purification