Systems biology solutions for biochemical production challenges.
There is an urgent need to significantly accelerate the development of microbial cell factories to produce fuels and chemicals from renewable feedstocks in order to facilitate the transition to a biobased society. Methods commonly used within the field of systems biology including omics characterization, genome-scale metabolic modeling, and adaptive laboratory evolution can be readily deployed in metabolic engineering projects. However, high performance strains usually carry tens of genetic modifications and need to operate in challenging environmental conditions. This additional complexity compared to basic science research requires pushing systems biology strategies to their limits and often spurs innovative developments that benefit fields outside metabolic engineering. Here we survey recent advanced applications of systems biology methods in engineering microbial production strains for biofuels and -chemicals.
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kgs., Lyngby, Denmark.
Anne Sofie Lærke
ASL
Hansen
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kgs., Lyngby, Denmark.
Rebecca M
RM
Lennen
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kgs., Lyngby, Denmark.
Nikolaus
N
Sonnenschein
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kgs., Lyngby, Denmark. Electronic address: herrgard@biosustain.dtu.dk.
Markus J
MJ
Herrgård
D056804:Biofuels; D019020:Directed Molecular Evolution / Q000379:methods*; D000072669:Gene Editing; D060847:Metabolic Engineering / Q000379:methods*; D058615:Synthetic Biology; D049490:Systems Biology / Q000379:methods*
D056804:Biofuels