Novel putative Bluetongue virus in healthy goats from Sardinia, Italy.
In recent years, novel Bluetongue virus (BTV) serotypes have been isolated and/or sequenced by researchers within the field. During Bluetongue surveillance activities, we identified a putative novel BTV serotype in healthy goats from Sardinia, Italy. RNAs purified from blood and serum samples were positive for BTV by a generic real time RT-PCR and c-ELISA, respectively, whereas genotyping and serotyping were unsuccessful. By NGS, the whole genome sequence was obtained from two blood samples (BTV-X ITL2015 strains 34200 and 33531). Overall, Seg 2 of BTV-X ITL2015 shows the highest identity (75.3-75.5% nt/77.4-78.1% aa) with recently isolated BTV-27s from Corsica and with the last discovered BTV XJ1407 from China (75.9% nt /78.2% aa), whereas it is less related with BTV-25 from Switzerland (73.0% nt/75.0% aa) and BTV-26 from Kuwait (62.0% nt/60.5% aa). A specific RT-qPCR targeting Seg 2 of BTV-X ITL2015 was assessed in this study. Considering the Seg 2/VP2 identity of BTV-X ITL2015 with BTV-25, 26, 27s and BTV XJ1407 and that serum of BTV-X ITL2015 infected goats failed to neutralize all tested extant serotypes, we propose the existence of a novel BTV serotype circulating in goats in Sardinia. Isolation was so far unsuccessful thus hampering proper antigenic characterization.
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Giovanni
G
Savini
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
Giantonella
G
Puggioni
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
Giorgio
G
Meloni
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Maurilia
M
Marcacci
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Marco
M
Di Domenico
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
Angela Maria
AM
Rocchigiani
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Massimo
M
Spedicato
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
Annalisa
A
Oggiano
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
Daniela
D
Manunta
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Liana
L
Teodori
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Alessandra
A
Leone
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Ottavio
O
Portanti
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Francesca
F
Cito
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Annamaria
A
Conte
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Massimiliano
M
Orsini
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Cesare
C
Cammà
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Paolo
P
Calistri
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Armando
A
Giovannini
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy. Electronic address: a.lorusso@izs.it.
Alessio
A
Lorusso
D000595:Amino Acid Sequence; D000818:Animals; D058070:Asymptomatic Diseases; D001819:Bluetongue / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D062665:Epidemiological Monitoring; D016679:Genome, Viral; D006041:Goats; D059014:High-Throughput Nucleotide Sequencing; D007106:Immune Sera / Q000737:chemistry; D007558:Italy / Q000453:epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D065288:Serogroup
D007106:Immune Sera; D012367:RNA, Viral
Bluetongue virus; Goats; New serotype; Next generation sequencing; Phylogeny; Sardinia