Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH).
Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high affinity probes for telomeric repeat sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.
Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d'Ulm, 75248, Paris, France.|UPMC Univ. Paris 06, F-75005, Paris, France.
Isabelle
I
Ourliac-Garnier
Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d'Ulm, 75248, Paris, France. arturo.londono@curie.fr.|UPMC Univ. Paris 06, F-75005, Paris, France. arturo.londono@curie.fr.
Arturo
A
Londoño-Vallejo
D002875:Chromosomes / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007399:Interphase / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D016615:Telomere / Q000235:genetics*
FISH; LNA; Length; PNA; Q-FISH; Telomere