Validated Method for the Screening and Quantification of Baclofen, Gabapentin and Pregabalin in Human Post-Mortem Whole Blood Using Protein Precipitation and Liquid Chromatography-Tandem Mass Spectrometry.
There has been a rapid increase in the number of prescriptions for baclofen (BLF), gabapentin (GBP) and pregabalin (PGL) in the UK since their introduction to therapy. Recent studies across the European Union and USA have shown the illicit abuse potential of these drugs and deaths have been observed. A simple, reliable and fully validated method was developed for the screening and quantification of BLF, GBP and PGL in human post-mortem (PM) blood. The analytes and their deuterated analogs as internal standard were extracted from blood using a single addition acetonitrile protein precipitation reaction followed by analysis using liquid chromatography-tandem mass spectrometry (LC-MS-MS) with triggered dynamic multiple reaction monitoring mode for simultaneous confirmation and quantification. The assay was linear from 0.05 to 1.00 µg/mL for BLF and 0.5 to 50.0 µg/mL for GBP and PGL, respectively with r2 > 0.999 (n = 9) for all analytes. Intra-day and inter-day imprecisions (n = 80) were calculated using one-way ANOVA; no significant difference (P > 0.99) was observed for all analytes over 8 non-consecutive days. The average recovery for all analytes was >98.9%. The limits of detection and quantification were both 0.05 µg/mL for BLF, and 0.5 µg/mL for GBP and PGL. The method was highly selective with no interference from endogenous compounds or from 54 drugs commonly encountered in PM toxicology. To prove method applicability, 17 PM blood samples submitted for analysis were successfully analyzed. The concentration range observed in PM blood for BLF was 0.08-102.00 µg/mL (median = 0.25 µg/mL), for GBP 1.0-134.0 µg/mL (median = 49.0 µg/mL) and 2.0-540.0 µg/mL (median = 42.0 µg/mL) for PGL.
Toxicology Unit, Imperial College London, London, United Kingdom of Great Britain and Northern Ireland.
Limon
L
Nahar
Toxicology Unit, Imperial College London, London, United Kingdom of Great Britain and Northern Ireland.
Amy
A
Smith
Toxicology Unit, Imperial College London, London, United Kingdom of Great Britain and Northern Ireland.
Rajan
R
Patel
Toxicology Unit, Imperial College London, London, United Kingdom of Great Britain and Northern Ireland.
Rebecca
R
Andrews
Toxicology Unit, Imperial College London, London, United Kingdom of Great Britain and Northern Ireland.
Sue
S
Paterson
D000588:Amines / Q000097:blood*; D001344:Autopsy / Q000379:methods*; D001418:Baclofen / Q000097:blood*; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003509:Cyclohexanecarboxylic Acids / Q000097:blood*; D000077206:Gabapentin; D006801:Humans; D000069583:Pregabalin / Q000097:blood*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D005680:gamma-Aminobutyric Acid / Q000097:blood*
D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D001418:Baclofen