Genetic Population Structure of Dastarcus helophoroides (Coleoptera: Bothrideridae) From Different Long-Horned Beetle Hosts Based on Complete Sequences of Mitochondrial COI.
Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important natural enemy of long-horned beetles in China, Japan, and Korea. In this study, the genetic sequence of cytochrome oxidase subunit Ι was used to investigate the genetics and relationships within and among D. helophoroides populations collected from five different geographic locations. We used principal component analysis, heatmap, and Venn diagram results to determine the relationship between haplotypes and populations. In total, 26 haplotypes with 51 nucleotide polymorphic sites were defined, and low genetic diversity was found among the different populations. Significant genetic variations were observed mainly within populations, and no correlation was found between genetic distribution and geographical distance. Low pairwise fixation index values (-0.01424 to 0.04896) and high gene flows show that there was high gene exchange between populations. The codistributed haplotype DH01 was suggested to be the most ancestral haplotype, and other haplotypes were thought to have evolved from it through several mutations. In four of the populations, both common haplotypes (DH01, DH03, and DH22) and unique haplotypes were found. Low genetic diversity among different populations is related to a relatively high flight capacity, host movement, and human-aided dispersal of D. helophoroides. The high gene exchange and typically weak population genetic structure among five populations, especially among populations of Anoplophora glabripennis (Motschulsky), Monochamus alternatus (Hope), and Massicus raddei (Blessig), may suggest that these populations cross naturally in the field.
Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, Republic of China (zhangzhengqing0802@hotmail.com; 1183934069@qq.com; limenglou@hotmail.com).
Zhengqing
Z
Zhang
Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, Republic of China (zhangzhengqing0802@hotmail.com; 1183934069@qq.com; limenglou@hotmail.com).
Yong
Y
Chang
Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, Republic of China ( zhangzhengqing0802@hotmail.com ; 1183934069@qq.com ; limenglou@hotmail.com ).|Corresponding author, e-mail: limenglou@hotmail.com.
Menglou
M
Li
D000818:Animals; D001483:Base Sequence; D002681:China; D001517:Coleoptera / Q000235:genetics* / Q000502:physiology; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D006239:Haplotypes; D019476:Insect Proteins / Q000235:genetics*; D007564:Japan; D024101:Mitochondrial Proteins / Q000235:genetics*; D010802:Phylogeny; D056910:Republic of Korea
D019476:Insect Proteins; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV
Dastarcus helophoroides; cytochrome oxidase subunit Ι; haplotype distribution; population genetic diversity