Linear Peptides Are the Major Products of a Biosynthetic Pathway That Encodes for Cyclic Depsipeptides.
Three new dentigerumycin analogues are produced by Streptomyces sp. M41, a bacterium isolated from a South African termite, Macrotermes natalensis. The structures of the complex nonribosomal peptide synthetase-polyketide synthase (NRPS/PKS) hybrid compounds were determined by 1D- and 2D-NMR spectroscopy, high-resolution mass spectrometry, and circular dichroism (CD) spectroscopy. Both cyclic and linear peptides are reported, and the genetic organization of the NRPS modules within the biosynthetic gene cluster accounts for the observed structural diversity.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts 02115, United States.|Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin , Madison, Wisconsin 53705, United States.
Thomas P
0000-0003-4703-741X
TP
Wyche
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Antonio C
AC
Ruzzini
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Christine
0000-0002-9747-3423
C
Beemelmanns
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Ki Hyun
0000-0002-5285-9138
KH
Kim
Department of Bacteriology, University of Wisconsin , Madison, Wisconsin 53706, United States.
Jonathan L
0000-0003-1745-8838
JL
Klassen
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Shugeng
S
Cao
Department of Bacteriology, University of Wisconsin , Madison, Wisconsin 53706, United States.
Michael
M
Poulsen
Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin , Madison, Wisconsin 53705, United States.
Tim S
0000-0002-4502-3084
TS
Bugni
Department of Bacteriology, University of Wisconsin , Madison, Wisconsin 53706, United States.
Cameron R
CR
Currie
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Jon
J
Clardy
D053898:Biosynthetic Pathways; D047630:Depsipeptides / Q000737:chemistry*; D015394:Molecular Structure; D005810:Multigene Family; D010453:Peptide Synthases; D048630:Polyketide Synthases; D013302:Streptomyces
D047630:Depsipeptides; D048630:Polyketide Synthases; D010453:Peptide Synthases