FunResDB-A web resource for genotypic susceptibility testing of Aspergillus fumigatus.
Therapy of invasive aspergillosis is becoming more difficult due to the emergence of azole resistance in Aspergillus fumigatus. A majority of resistant strains carries mutations in the CYP51A gene. Due to a lack of sensitivity of culture-based methods, molecular detection of A. fumigatus has become an important diagnostic tool. We set up the database FunResDB (www.nrz-myk.de/funresdb) to gather all available information about CYP51A-dependent azole resistance from published literature. In summary, the screening resulted in 79 CYP51A variants, which are linked to 59 nonsynonymous mutations. A tailor-made online sequence analysis tool allows for genotypic susceptibility testing of A. fumigatus.
German National Reference Center for Invasive Fungal Infections - NRZMyk, Septomics Research Centre, Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, 07745 Jena, Germany.
Michael
M
Weber
German National Reference Center for Invasive Fungal Infections - NRZMyk, Septomics Research Centre, Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, 07745 Jena, Germany.
Jonas
J
Schaer
German National Reference Center for Invasive Fungal Infections - NRZMyk, Septomics Research Centre, Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, 07745 Jena, Germany.
Grit
G
Walther
German National Reference Center for Invasive Fungal Infections - NRZMyk, Septomics Research Centre, Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, 07745 Jena, Germany.
Kerstin
K
Kaerger
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Jörg
J
Steinmann
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Peter-Michael
PM
Rath
Department of Hematology and Oncology, Mannheim University Hospital, University of Heidelberg, Mannheim, Germany.
Birgit
B
Spiess
Department of Hematology and Oncology, Mannheim University Hospital, University of Heidelberg, Mannheim, Germany.
Dieter
D
Buchheidt
Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany.
Axel
A
Hamprecht
German National Reference Center for Invasive Fungal Infections - NRZMyk, Septomics Research Centre, Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, 07745 Jena, Germany.|Center for Sepsis Control and Care, University Hospital Jena, Jena, Germany.
Oliver
O
Kurzai
D000483:Alleles; D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000235:genetics*; D001393:Azoles / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D030541:Databases, Genetic; D005656:Fungal Proteins / Q000235:genetics; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D020407:Internet; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011110:Polymorphism, Genetic
D000935:Antifungal Agents; D001393:Azoles; D005656:Fungal Proteins; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus
Aspergillus fumigatus; database; drug susceptibility; fungal infection; triazoles