Comparison of Cobas® HPV and Anyplex™ II HPV28 assays for detecting and genotyping human papillomavirus.
Persistent infection with high-risk human papillomavirus (HPV-HR) is a recognized cause of cervical cancer. The aim of this study was to compare analytical and clinical performances of the Cobas® HPV and Anyplex™ II HPV28 assays for HPV detection and genotyping. A total of 94 cervical samples were tested. For HPV-HR, the results agreed very well (94.68%), with 100% agreement when detecting CIN2+. The Anyplex™ II HPV28 assay detected more genotypes than the Cobas® HPV Test, but their clinical performances were similar.
INSERM U1043, CPTP, CHU Purpan, BP 3028, F-31024 Toulouse, France; Université de Toulouse, UPS, CPTP, F-31024 Toulouse, France; Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, F-31059 Toulouse, France. Electronic address: pasquier.c@chu-toulouse.fr.
Christophe
C
Pasquier
INSERM U1043, CPTP, CHU Purpan, BP 3028, F-31024 Toulouse, France; Université de Toulouse, UPS, CPTP, F-31024 Toulouse, France; Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, F-31059 Toulouse, France.
Karine
K
Sauné
INSERM U1043, CPTP, CHU Purpan, BP 3028, F-31024 Toulouse, France; Université de Toulouse, UPS, CPTP, F-31024 Toulouse, France; Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, F-31059 Toulouse, France.
Stéphanie
S
Raymond
Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, F-31059 Toulouse, France.
Jérôme
J
Boisneau
Université de Toulouse, UPS, CPTP, F-31024 Toulouse, France; Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, F-31059 Toulouse, France; Pathology and Cytology Department, CHU de Toulouse, IUCT, F-31059 Toulouse, France.
Monique
M
Courtade
INSERM U1043, CPTP, CHU Purpan, BP 3028, F-31024 Toulouse, France; Université de Toulouse, UPS, CPTP, F-31024 Toulouse, France; Laboratoire de Virologie, CHU de Toulouse, Hôpital Purpan, F-31059 Toulouse, France.
Jacques
J
Izopet
D000328:Adult; D000368:Aged; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D055815:Young Adult
Anyplex™ II HPV28; HPV genotyping; Human papillomavirus; Real-time PCR