Par3 in chick lens placode development.
The lens originates from a simple cuboidal epithelium, which, on its basal side, contacts the optic vesicle, whilst facing the extraembryonic environment on its apical side. As this epithelium changes into the pseudostratified lens placode, its cells elongate and become narrower at their apical ends. This is due to the formation of an apical actin network, whose appearance is restricted to cells of the placodal region, as a result of region-specific signaling mechanisms that remain largely unknown. Here, we investigated the role of the polarity protein PAR3 and the phosphorylation state of its Threonine 833 (T833) aPKC-binding site in the recruitment of aPKC and in the establishment of actin network in the chick lens placode. Overexpression of wild type PAR3 recruited aPKC and punctate actin clusters to the basolateral membranes of the placodal cells. Recruitment of aPKC depended on the charge of the residue that replaced the T833 residue. In contrast, induction of the ectopic actin spots was independent on the charge of this residue.
Department of Cell and Developmental Biology, Institute for Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, São Paulo, SP, 05508-900, Brazil.
Maraysa de Oliveira
MO
Melo
Department of Cell and Developmental Biology, Institute for Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, São Paulo, SP, 05508-900, Brazil.
Ricardo
R
Moraes Borges
Department of Cell and Developmental Biology, Institute for Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, São Paulo, SP, 05508-900, Brazil.
Chao Yun Irene
0000-0003-0527-5719
CYI
Yan
D000199:Actins / Q000378:metabolism; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D016764:Cell Polarity; D002642:Chick Embryo; D004475:Ectoderm / Q000196:embryology / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D007908:Lens, Crystalline / Q000196:embryology / Q000378:metabolism*; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism
D000199:Actins; D018797:Cell Cycle Proteins; D011493:Protein Kinase C
Avian; early development; organism process; placodes; process; signaling; tissue