Global Functional Analysis of Butanol-SensitiveEscherichia coliand Its Evolved Butanol-Tolerant Strain.
Butanol is a promising alternative to ethanol and is desirable for use in transportation fuels and additives to gasoline and diesel fuels. Microbial production of butanol is challenging primarily because of its toxicity and low titer of production. Herein, we compared the transcriptome and phenome of wild-typeEscherichia coliand its butanol-tolerant evolved strain to understand the global cellular physiology and metabolism responsible for butanol tolerance. When the ancestral butanol-sensitiveE. coliwas exposed to butanol, gene activities involved in respiratory mechanisms and oxidative stress were highly perturbed. Intriguingly, the evolved butanol-tolerant strain behaved similarly in both the absence and presence of butanol. Among the mutations occurring in the evolved strain, cis-regulatory mutations may be the cause of butanol tolerance. This study provides a foundation for the rational design of the metabolic and regulatory pathways for enhanced biofuel production.
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Haeyoung
H
Jeong
SeqGenesis, Daejeon 34111, Republic of Korea.
Seung-Won
SW
Lee
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Sun Hong
SH
Kim
School of Basic Sciences, Hanbat National University, Daejeon 34158, Republic of Korea.
Eun-Youn
EY
Kim
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
Sinyeon
S
Kim
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
Sung Ho
SH
Yoon
D020001:1-Butanol / Q000378:metabolism* / Q000494:pharmacology*; D056804:Biofuels; D004361:Drug Tolerance; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D060847:Metabolic Engineering; D055432:Metabolomics; D009154:Mutation; D010641:Phenotype; D059467:Transcriptome
D056804:Biofuels; D000431:Ethanol; D020001:1-Butanol
Butanol; Escherichia coli; evolution; tolerance; transcriptome