Pd-Au@carbon dots nanocomposite: Facile synthesis and application as an ultrasensitive electrochemical biosensor for determination of colitoxin DNA in human serum.
In this study, a green and fast method was developed to synthesize high-yield carbon dots (CDs) via one-pot microwave treatment of banana peels without using any other surface passivation agents. Then the as-prepared CDs was used as the reducing agent and stabilizer to synthesize a Pd-Au@CDs nanocomposite by a simple sequential reduction strategy. Finally, Pd-Au@CDs nanocomposite modified glassy carbon electrode (Pd-Au@CDs/GCE) was obtained as a biosensor for target DNA after being immobilized a single-stranded probe DNA by a carboxyl ammonia condensation reaction. Under the optimal conditions, the sensor could detect target DNA concentrations in the range from 5.0×10-16to 1.0×10-1°molL-1. The detection limit (LD) was estimated to be 1.82×10-17molL-1, which showed higher sensitivity than other electrochemical biosensors reported. In addition, the DNA sensor was also successfully applied to detect colitoxin DNA in human serum.
Department of Chemistry, Shantou University, Shantou 515063, PR China; Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, PR China.
Qitong
Q
Huang
Department of Chemistry, Shantou University, Shantou 515063, PR China.
Xiaofeng
X
Lin
Department of Chemistry, Shantou University, Shantou 515063, PR China.
Jie-Ji
JJ
Zhu
Department of Chemistry, Shantou University, Shantou 515063, PR China. Electronic address: qxtong@stu.edu.cn.
Qing-Xiao
QX
Tong
D000641:Ammonia / Q000737:chemistry; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
D010087:Oxides; D002244:Carbon; D006046:Gold; D000641:Ammonia; D004247:DNA
Banana peels; Carbon dots; DNA biosensor; Pd-Au@Carbon dots nanocomposite