Effect of Melilotus officinalis extract on the apoptosis of brain tissues by altering cerebral thrombosis and inflammatory mediators in acute cerebral ischemia.
Present investigation evaluates the protective effect of Melilotus officinalis (MO) extract on the brain tissues in acute cerebral ischemia. Acute cerebral ischemia was induced by occlusion of carotid artery and rats with cerebral ischemia were treated with MO (100, 250 & 500mg/kg) for the duration of three days. Cerebral ischemia was confirmed by estimating infract volume and neurological deficit score. Moreover biochemical parameters in plasma such as 6-keto-PGF1α and TXB2 and concentration of cytokine, oxidative stress, apoptosis ratio and protein expressions of Bcl2 & Bax were estimated in the brain tissues. It was observed that treatment with MO significantly (p<0.01) decreases the infract volume and neurological deficit score than negative control group. There was significant decrease (p<0.01) in the oxidative stress and cytokine in the brain tissues and increase in the plasma concentration of 6-keto-PGF1α in MO treated group of rats compared to negative control group. Plasma concentration of TXB 2 was significantly enhanced in MO treated group compared to negative control group of rats. It was also found that treatment with MO ameliorates the apoptosis induced by cerebral ischemia. Present study concludes that MO ameliorates apoptosis of brain tissues in cerebral ischemic rats by decreasing cerebral thrombosis, oxidative stress and inflammatory mediators.
Department of Neurology, Shanxian Dongda Hospital, Shanxian 274300, Shandong, China.
Gui-Cun
GC
Zhao
Department of Neurology, Shanxian Dongda Hospital, Shanxian 274300, Shandong, China.
Yan-Ling
YL
Yuan
Department of Neurology, Chengwu Hospital of Traditional Chinese Medicine, Chengwu 274200, Shandong, China.
Feng-Rong
FR
Chai
Department of Neurology, Shanxian Central Hospital, Shanxian 274300, Shandong, China. Electronic address: fajingji1985@hotmail.com.
Fa-Jing
FJ
Ji
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D002544:Cerebral Infarction / Q000188:drug therapy / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000494:pharmacology*; D020767:Intracranial Thrombosis / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D029903:Melilotus / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D016207:Cytokines; D018836:Inflammation Mediators; D018696:Neuroprotective Agents; D010936:Plant Extracts
Apoptosis; Cerebral ischemia; Cytokines; Melilotus officinalis (MO); Oxidative stress