2D-Electrophoresis and Immunoblotting in Food Allergy.
Two-dimensional polyacrylamide gel electrophoresis (2DE) and Western immunoblotting have proven to be invaluable and fundamental techniques for comprehensive characterization of food allergens. Here, we describe and discuss detailed protocols used in our studies on identification of allergenic proteins in shrimp, sesame, hazelnut, and pistachio.
Elliot and Roslyn Jaffe Food Allergy Institute, Division of Allergy and Immunology, Department of Pediatrics, Icahn School of Medicine at Mount Sinai, 1 Gustave L Levy Place, New York, 10029, NY, USA. galina.grishina@mssm.edu.
Galina
G
Grishina
Elliot and Roslyn Jaffe Food Allergy Institute, Division of Allergy and Immunology, Department of Pediatrics, Icahn School of Medicine at Mount Sinai, 1 Gustave L Levy Place, New York, 10029, NY, USA.
Luda
L
Bardina
Elliot and Roslyn Jaffe Food Allergy Institute, Division of Allergy and Immunology, Department of Pediatrics, Icahn School of Medicine at Mount Sinai, 1 Gustave L Levy Place, New York, 10029, NY, USA.
Alexander
A
Grishin
D000485:Allergens / Q000032:analysis* / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005502:Food; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods
D000485:Allergens
2D electrophoresis; Food allergen; Hazelnut; Immunoblotting; Pistachio; Proteomics