Comparison of binding interaction between β-lactoglobulin and three common polyphenols using multi-spectroscopy and modeling methods.
Tea, coffee and fruit in dairy products are rich in polyphenols. The interaction mechanism between β-lactoglobulin (β-LG) and chlorogenic acid (CGA), ferulic acid (FA) and epigallocatechin-3-gallate (EGCG) was investigated. Fluorescence experiments proved that polyphenols quenched β-LG fluorescence strongly in static mode and EGCG had stronger binding affinity toward β-LG than CGA and FA. The main interaction force of EGCG binding with β-LG was different from CGA and FA. Furthermore, circular dichroism and fourier transform infrared data indicated that polyphenols changed β-LG secondary structure inducing a-helix to β-structures transition. The surface hydrophobicity of β-LG was also changed slightly by them according to surface hydrophobicity and particle size experiments. These results showed that the interaction mechanism of β-LG with phenolic acid esters was different from it with phenolic acids. Besides, polyphenols had impact on the structure and functionality of β-LG, which would be valuable in dairy processing industry.
School of Life Science, Shandong Normal University, Jinan, PR China.
Jingjing
J
Jia
School of Life Science, Shandong Normal University, Jinan, PR China.
Xin
X
Gao
School of Life Science, Shandong Normal University, Jinan, PR China.
Minghao
M
Hao
School of Life Science, Shandong Normal University, Jinan, PR China. Electronic address: tanglin@sdnu.edu.cn.
Lin
L
Tang
D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D002942:Circular Dichroism; D003373:Coumaric Acids / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*
D003373:Coumaric Acids; D007782:Lactoglobulins; D059808:Polyphenols; D002392:Catechin; C004999:ferulic acid; C045651:epigallocatechin gallate
8-Anilino-1-naphthalenesulfonic acid (PubChem CID: 1369); Chlorogenic acid; Chlorogenic acid (PubChem CID: 1794427); Circular dichroism; Disodium hydrogen phosphate (PubChem CID: 24203); Epigallocatechin-3-gallate; Epigallocatechin-3-gallate (PubChem CID: 65064); Ferulic acid; Ferulic acid (PubChem CID: 445858); Fluorescence; Monosodium phosphate (PubChem CID: 23672064); β-Lactoglobulin