Effects of different cellulases on the release of phenolic acids from rice straw during saccharification.
Effects of different cellulases on the release of phenolic acids from rice straw during saccharification were investigated in this study. All cellulases tested increased the contents of phenolic acids during saccharification. However, few free phenolic acids were detected, as they were present in conjugated form after saccharification when the cellulases from Trichoderma reesei, Trichoderma viride and Aspergillus niger were used. On the other hand, phenolic acids were present in free form when the Acremonium cellulolyticus cellulase was used. Assays of enzyme activity showed that, besides high cellulase activity, the A. cellulolyticus cellulase exhibited high feruloyl esterase (FAE) activity. A synergistic interaction between FAE and cellulase led to the increase in free phenolic acids, and thus an increase in antioxidative and antiradical activities of the phenolic acids. Moreover, a cost estimation demonstrated the feasibility of phenolic acids as value-added products to reduce the total production cost of ethanol.
Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
Yiyun
Y
Xue
Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
Xiahui
X
Wang
Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
Xingxuan
X
Chen
Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
Jiajun
J
Hu
Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China; Energy Research Institute of Shandong Academy of Science, Jinan 250014, China. Electronic address: mtgao@shu.edu.cn.
Min-Tian
MT
Gao
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 20110, China.
Jixiang
J
Li
D001234:Aspergillus niger; D002480:Cellulase; D044602:Cellulases; D012275:Oryza; D014242:Trichoderma / Q000201:enzymology
D044602:Cellulases; D002480:Cellulase
Antioxidant capacity; Cellulase; Feruloyl esterase; Phenolic acid; Rice straw