The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats.
Objective. To investigate the effects of Tong-Xie-Yao-Fang (TXYF) on intestinal mucosal mast cells in rats with postinfectious irritable bowel syndrome (PI-IBS).Design. PI-IBS rat models were established using a multistimulation paradigm. Then, rats were treated with TXYF intragastrically at doses of 2.5, 5.0, and 10.0 g·kg-1·d-1for 14 days, respectively. Intestinal sensitivity was assessed based on abdominal withdrawal reflex (AWR) scores and fecal water content (FWC). Mast cell counts and the immunofluorescence of tryptase and c-Fos in intestinal mucosa were measured; and serum IL-1β, TNF-α, and histamine levels were determined.Results. AWR reactivity and FWC which were significantly increased could be observed in PI-IBS rats. Remarkably increased mast cell activation ratio in intestinal mucosa, together with increased serum TNF-αand histamine levels, could also be seen in PI-IBS rats; furthermore, PI-IBS-induced changes in mast cell activation and level of serum TNF-αand histamine could be reversed by TXYF treatment. Meanwhile, tryptase and c-Fos expression were also downregulated.Conclusion. TXYF improves PI-IBS symptoms by alleviating behavioral hyperalgesia and antidiarrhea, the underlying mechanism of which involves the inhibitory effects of TXYF on activating mucosal mast cells, downregulating tryptase and c-Fos expression, and reducing serum TNF-αand histamine levels.
Graduate School, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China; Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Xiangxue
0000-0002-9214-2107
X
Ma
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China; The First Affiliated Hospital of Henan University of TCM, Zhengzhou, Henan 450000, China.
Xiaoge
X
Wang
Graduate School, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China; Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Nan
N
Kang
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Ting
T
Chen
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Haijie
H
Ji
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Lin
0000-0003-4474-1938
L
Lv
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Xiaolan
X
Yin
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Yaxin
Y
Tian
Department of Pharmaceutical Preparation, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Beijing 100091, China.
Rui
R
Zheng
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Yuanzhi
Y
Duan
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Fengyun
0000-0002-4058-7905
F
Wang
Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xi Yuan Yard Road, Haidian District, Beijing 100091, China.
Xudong
0000-0003-0391-3895
X
Tang