Hyperbaric oxygen therapy reduces renal lactate production.
Intrarenal hypoxia is an acknowledged factor contributing to the development of diabetic nephropathy. Hyperbaric oxygen (HBO) therapy is a well-known adjuvant treatment for several medical conditions, such as decompression sickness, infections, and wound healing. The underlying metabolic response of HBO is largely unknown. In this study, we investigated the effect of HBO on the intrarenal metabolic alteration in diabetes. Hyperpolarized [1-13C]pyruvate MRI was performed to assess intrarenal energy metabolism in normoglycemic controls and short-term (2 weeks) streptozotocin-induced diabetic rats with and without HBO for five consecutive days. HBO therapy blunted intrarenal lactate production, 3 days after the therapy, in both normoglycemic controls and diabetic rats without affecting either lactate dehydrogenase mRNA expression or activity. HBO therapy reduced lactate formation in both normoglycemic and hyperglycemic rats. These findings support hyperpolarized [1-13C]pyruvate MRI as a novel method for monitoring HBO therapy via the pyruvate to lactate conversion.
Department of Clinical Medicine, MR Research Centre, Aarhus University, Aarhus, Denmark.
Thomas S
TS
Nørlinger
Department of Clinical Medicine, MR Research Centre, Aarhus University, Aarhus, Denmark.
Per Mose
PM
Nielsen
Department of Clinical Medicine, MR Research Centre, Aarhus University, Aarhus, Denmark.
Haiyun
H
Qi
Department of Clinical Medicine, MR Research Centre, Aarhus University, Aarhus, Denmark.
Emmeli
E
Mikkelsen
Department of Clinical Medicine, Comparative Medicine Lab, Aarhus University, Aarhus, Denmark.
Kasper
K
Hansen
Department of Clinical Medicine, Comparative Medicine Lab, Aarhus University, Aarhus, Denmark.
Nikolaj H
NH
Schmidt
Department of Clinical Medicine, MR Research Centre, Aarhus University, Aarhus, Denmark.|Department of Clinical Medicine, Comparative Medicine Lab, Aarhus University, Aarhus, Denmark.
Michael
M
Pedersen
Department of Pediatrics, Aarhus University Hospital, Aarhus, Denmark.|Department of Clinical Medicine, Comparative Medicine Lab, Aarhus University, Aarhus, Denmark.
Peter
P
Agger
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
Fredrik
F
Palm
Department of Clinical Medicine, MR Research Centre, Aarhus University, Aarhus, Denmark cl@clin.au.dk.
Christoffer
C
Laustsen
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism* / Q000628:therapy; D005260:Female; D006931:Hyperbaric Oxygenation; D007668:Kidney / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D019344:Lactic Acid

MRI
; Hyperpolarization; kidney; kidney metabolism; type 1 diabetes