Ultrasensitive immunosensor for prostate specific antigen using biomimetic polydopamine nanospheres as an electrochemiluminescence superquencher and antibody carriers.
In this work, biomimetic polydopamine nanospheres (PDANSs) were easily prepared and were firstly exploited as an electrochemiluminescence (ECL) nanoquencher for the development of a sandwich type immunosensor. The PDANSs with abundant active functional groups can facilely label the detection antibody and show remarkable quenching effect towards the ECL of the tris-(2,2'-bipyridine)ruthenium (Ru(bpy)32+). The amino-modified multiwall carbon nanotubes/Nafion (MWCNTs-NH2@N) composite-film was adopted as a matrix to incorporate the luminophor Ru(bpy)32+and immobilize the capture antibody. The prominent decrease of ECL signal intensity was obtained on account of the unique quenching ability of the labeled PDANSs. The quenching mechanism is believed that the excited states of Ru(bpy)32+can be annihilated by quinone units in PDANSs via energy transfer. The ECL quenching efficiency was logarithmically related to the concentration of the prostate specific antigen (PSA) in the range from 0.1 pg mL-1to 20 ng mL-1with a detection limit of 35 fg mL-1. Furthermore, the proposed ECL immunosensor presented good stability, repeatability and selectivity.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Yuanyuan
Y
Liu
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Yanhua
Y
Zhao
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Zongwei
Z
Zhu
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Zhenyuan
Z
Xing
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: mahongmin2002@126.com.
Hongmin
H
Ma
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Qin
Q
Wei
D055910:Antibodies, Immobilized / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D008163:Luminescent Measurements; D054118:Nanospheres / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000097:blood
D055910:Antibodies, Immobilized; D005465:Fluorocarbon Polymers; D007211:Indoles; D037742:Nanotubes, Carbon; D009942:Organometallic Compounds; D011108:Polymers; C568283:polydopamine; C415768:tris(2,2'-bipyridyl)ruthenium(II); C040402:perfluorosulfonic acid; D017430:Prostate-Specific Antigen
Electrochemiluminescence; Polydopamine nanospheres; Quenching; Ru(bpy)(3)(2+)