The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen.
We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals.
Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand. v.sander@auckland.ac.nz.
Veronika
V
Sander
Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand. spat261@aucklanduni.ac.nz.
Shantanu
S
Patke
Department of Chemistry & NUS MedChem Program of Life Sciences Institute, National University of Singapore and Singapore Bioimaging Consortium, A* STAR, Singapore 138667, Singapore. lsilj@nus.edu.sg.
Jung Y
JY
Lee
Department of Chemistry & NUS MedChem Program of Life Sciences Institute, National University of Singapore and Singapore Bioimaging Consortium, A* STAR, Singapore 138667, Singapore. chmcyt@nus.edu.sg.
Young-Tae
YT
Chang
Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand. a.davidson@auckland.ac.nz.
Alan J
AJ
Davidson
D000818:Animals; D001896:Boron Compounds / Q000008:administration & dosage*; D007422:Intestines / Q000648:ultrastructure*; D007814:Larva / Q000033:anatomy & histology; D008856:Microscopy, Fluorescence; D015398:Signal Transduction; D014212:Tretinoin / Q000378:metabolism; D015027:Zebrafish / Q000033:anatomy & histology* / Q000254:growth & development
D001896:Boron Compounds; C000591023:CDr10b compound; D014212:Tretinoin
CDr10b; gut development; laser ablation; mid-intestine; vital dye; zebrafish