Novel immunochromatographic assay on cotton thread based on carbon nanotubes reporter probe.
In this article, a novel immunochromatographic assay method on cotton thread based on carbon nanotubes (CNTs) as reporter probe was successfully prepared for visual and rapid detection of a lung cancer related biomarker, human ferritin antigen. A model system comprising ferritin as an analyte was used to demonstrate the protocol of the cotton thread immunoassay device. The device can detect at least 50ng/mL human ferritin antigen, which improved the sensitivity approximately by 500 folds comparing with previous point-of-care test report based on carbon nanotubes as reporter probe. The CNTs reporter probe combined with cotton thread device based biosensor provided an alternative path for clinical diagnosis of other protein or nucleic acid biomarkers.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi Province 710127, PR China.
Li-Li
LL
Meng
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi Province 710127, PR China.
Ting-Ting
TT
Song
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi Province 710127, PR China. Electronic address: xunmao@nwu.edu.cn.
Xun
X
Mao
D002846:Chromatography, Affinity / Q000379:methods*; D032561:Cotton Fiber; D005293:Ferritins / Q000032:analysis / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D037742:Nanotubes, Carbon / Q000737:chemistry*
D037742:Nanotubes, Carbon; D005293:Ferritins
Carbon nanotubes; Cotton thread immunochromatographic assay device; Point-of-care diagnosis; Protein detection