Optimization of selective pressurized liquid extraction of organic pollutants in placenta to evaluate prenatal exposure.
The early exposure to organic pollutants (OPs) related to dietary habit or environmental exposure is a concern of growing interest in environmental health. When OPs enter the body, they can accumulate in fatty tissue and even travel through the bloodstream being able to cross the placenta and reach the fetus through the substance exchange between the mother and the child. Epidemiologic and clinical data affirm that these chemicals increase the risk of adverse effects during childhood. This article reviews and addresses one of the most relevant analytical methods for determining OPs in placenta. We discuss and critically evaluate procedures, such as solid-liquid and selective pressurized liquid extraction (SPLE). Clean-up of extracts was performed by solid-phase extraction (SPE) using EZ-POP cartridges. Detection of OPs was carried out by gas chromatography (GC) coupled to tandem mass spectrometry (MS/MS). Recoveries ranged from 52% to 94% by SPLE with estimated quantification limits between 0.15 and 2.2ng/g for organochlorine pesticides (OCPs), between 0.083 and 0.50 for organophosphate pesticides (OPPs), between 0.025 and 0.15ng/g for polychlorinated biphenyl (PCBs), between 0.015 and 0.10ng/g for polybromodiphenyl ethers (PBDEs), between 0.33 and 0.66ng/g for pyrethroids and between 0.022 and 0.87ng/g for polycyclic aromatic hydrocarbons (PAHs). Most of the target OPs were detected in twenty real placenta samples studied, with pyrethroids, PAHs and OPPs representing most of the 90% of OPs with means of 13-18ng/g versus PCBs, OCPs, and PBDEs with means <4.0ng/g. The rsults of this work indicate a prenatal exposure to OPs in Galicia.
Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, 32400 Ourense, Spain. Electronic address: fcruz@uvigo.es.
Tania
T
Fernández-Cruz
Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, 32400 Ourense, Spain. Electronic address: elena.martinez@uvigo.es.
Elena
E
Martínez-Carballo
Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, 32400 Ourense, Spain. Electronic address: jsimal@uvigo.es.
Jesús
J
Simal-Gándara
D004785:Environmental Pollutants / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000032:analysis; D059625:Liquid-Liquid Extraction; D018811:Maternal Exposure; D010575:Pesticides / Q000032:analysis / Q000302:isolation & purification; D010920:Placenta / Q000737:chemistry* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000302:isolation & purification; D011247:Pregnancy; D011312:Pressure; D052616:Solid Phase Extraction
D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D011078:Polychlorinated Biphenyls
Adverse effects; Organic pollutants; Placenta; selective pressurized liquid extraction