A novel vitivirus-like sequence found in Arracacia xanthorrhiza plants by high throughput sequencing.
High throughput sequencing (HTS) is a very powerful tool for detecting and discovering novel viral-like sequences without prior knowledge of the sequence. Here we describe the complete genome of a new vitivirus-like sequence that was found in arracacha (Arracacia xanthorrhiza) plants using HTS technology. The complete genome sequence was validated by Sanger sequencing. The genomic organization of the new putative vitivirus resembles that of grapevine virus B (GVB) and grapevine virus D (GVD). The putative coat protein showed 41 to 49% identity with similar proteins of known vitiviruses, while the RNA-dependent RNA polymerase shared 52 to 55% identity with those encoded by grapevine vitiviruses. Based on the demarcation criteria for the genus Vitivirus, the virus described in this work, provisionally named as "Arracacha virus V", represents a novel species in this taxon.
Department Cell Biology, University of Brasília, 70910-900, Brasília, DF, Brazil.
Layssa M
LM
Oliveira
Department Cell Biology, University of Brasília, 70910-900, Brasília, DF, Brazil.
Anelise F
AF
Orílio
Embrapa Vegetables, Caixa Postal 218, 70275-970, Brasília, DF, Brazil.
Alice K
AK
Inoue-Nagata
Department Cell Biology, University of Brasília, 70910-900, Brasília, DF, Brazil.
Tatsuya
T
Nagata
Department Cell Biology, University of Brasília, 70910-900, Brasília, DF, Brazil. rblawid@gmail.com.
Rosana
http://orcid.org/0000-0003-1001-7191
R
Blawid
D019661:Apiaceae / Q000821:virology*; D052677:Flexiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics
D012324:RNA-Dependent RNA Polymerase