Zinc and Copper Effects on Stability of Tubulin and Actin Networks in Dendrites and Spines of Hippocampal Neurons.
Zinc and copper ions can modulate the activity of glutamate receptors. However, labile zinc and copper ions likely represent only the tip of the iceberg and other neuronal functions are suspected for these metals in their bound state. We performed synchrotron X-ray fluorescence imaging with 30 nm resolution to image total biometals in dendrites and spines from hippocampal neurons. We found that zinc is distributed all along the dendrites while copper is mainly pinpointed within the spines. In spines, zinc content is higher within the spine head while copper is higher within the spine neck. Such specific distributions suggested metal interactions with cytoskeleton proteins. Zinc supplementation induced the increase of β-tubulin content in dendrites. Copper supplementation impaired the β-tubulin and F-actin networks. Copper chelation resulted in the decrease of F-actin content in dendrites, drastically reducing the number of F-actin protrusions. These results indicate that zinc is involved in microtubule stability whereas copper is essential for actin-dependent stability of dendritic spines, although copper excess can impair the dendritic cytoskeleton.
University of Bordeaux, CENBG, UMR 5797, F-33170 Gradignan, France.|CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan, France.
Laura
L
Perrin
University of Bordeaux, CENBG, UMR 5797, F-33170 Gradignan, France.|CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan, France.
Stéphane
S
Roudeau
University of Bordeaux, CENBG, UMR 5797, F-33170 Gradignan, France.|CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan, France.
Asuncion
A
Carmona
University of Bordeaux, CENBG, UMR 5797, F-33170 Gradignan, France.|CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan, France.|University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, 33000 Bordeaux, France.|CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, 33000 Bordeaux, France.
Florelle
F
Domart
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, 33000 Bordeaux, France.|CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, 33000 Bordeaux, France.|Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, University of Bordeaux, 33000 Bordeaux, France.
Jennifer D
JD
Petersen
ESRF, The European Synchrotron , 38000 Grenoble, France.|Inserm U1216, 38000 Grenoble, France.|Grenoble Institut des Neurosciences, GIN University of Grenoble Alpes , 38000 Grenoble, France.
Sylvain
S
Bohic
ESRF, The European Synchrotron , 38000 Grenoble, France.
Yang
Y
Yang
ESRF, The European Synchrotron , 38000 Grenoble, France.
Peter
0000-0002-4129-9091
P
Cloetens
University of Bordeaux, CENBG, UMR 5797, F-33170 Gradignan, France.|CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan, France.
Richard
0000-0003-1692-5406
R
Ortega
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes; D002412:Cations / Q000378:metabolism; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D018920:Coculture Techniques; D003300:Copper / Q000008:administration & dosage / Q000378:metabolism*; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism*; D046169:Dermoscopy; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D013052:Spectrometry, X-Ray Emission; D014404:Tubulin / Q000378:metabolism*; D015032:Zinc / Q000008:administration & dosage / Q000378:metabolism*
D000199:Actins; D002412:Cations; D002614:Chelating Agents; D014404:Tubulin; D003300:Copper; D015032:Zinc
X-ray fluorescence; Zinc; actin; copper; element imaging; hippocampus; nanoscopy; neuron; spine; synchrotron; tubulin