Thermal inactivation of foot and mouth disease virus in extruded pet food.
The risk of importing foot and mouth disease, a highly contagious viral disease of livestock, severely restricts trade and investment opportunities in many developing countries where the virus is present. This study was designed to investigate the inactivation of foot and mouth disease virus (FMDV) by heat treatments used in extruded commercial pet food manufacture. If extrusion could be shown to reliably inactivate the virus, this could potentially facilitate trade for FMDV-endemic countries. The authors found that there was no detectable virus following: i) treatment of FMDVspiked meat slurry at 68°C for 300 s; ii) treatment of FMDV-spiked slurry and meal mix at 79°C for 10 or 30 s, or iii) treatment of homogenised bovine tongue epithelium, taken from an FMDV-infected animal, at 79°C for 10 s. This corresponds to an estimated 8 log10 reduction in titre (95% credible interval: 6 log10 -13 log10). Furthermore, the authors found that the pH of the slurry and meal mix was sufficient to inactivate FMDV in the absence of heat treatment. This demonstrates that heat treatments used in commercial pet food manufacture are able to substantially reduce the titre of FMDV in infected raw materials.
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S
Gubbins
J
J
Forster
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S
Clive
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D
Schley
S
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Zuber
J
J
Schaaff
D
D
Corley
D000821:Animal Feed / Q000592:standards / Q000821:virology*; D000818:Animals; D004848:Epithelium / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D013997:Time Factors; D014059:Tongue / Q000821:virology
Cat; Dog; Extrusion; FMDV; Foot and mouth disease virus; Heat treatment; Pet food