The cessation of breathing in the chicken embryo during cold-hypometabolism.
The avian embryo toward end-incubation combines gas exchange through the chorioallantoic membrane (CAM) and pulmonary ventilation (V˙E). The main experiments examined breathing activity during cold-hypometabolism. Chicken embryos close to hatching were prepared for simultaneous measurements of oxygen consumption ( [Formula: see text] ) and carbon dioxide production ( [Formula: see text] ; open-flow methodology) and breathing frequency (f; barometric technique). As ambient (Ta) and egg temperature (Tegg) dropped, breathing eventually ceased at ∼18°C, when [Formula: see text] and [Formula: see text] were 22-28% of the normothermic values. With the eggshell experimentally covered to reduce CAM gas exchange breathing ceased at slightly lower [Formula: see text] and [Formula: see text] (17-18% of normothermia). Once breathing had stopped, egg exposure to hypoxia (10% or 5% O2) or hypercapnia (3% or 8% CO2) did not resume breathing, which recovered with re-warming. In normothermia, 10% O2caused hypometabolism and tachypnea; differently, in 5% O2[Formula: see text] dropped as much as with hypothermia and breathing stopped, to recover upon return in air. Correlation analysis among Ta, Tegg, [Formula: see text] , [Formula: see text] and f during cooling and re-warming indicated that f followed more closely the changes in [Formula: see text] and, especially, in [Formula: see text] than the changes in Ta or Tegg. Some considerations suggest that in this experimental model the cessation of breathing in hypothermia or severe hypoxia may be due to hypometabolism, while the lack of chemo-responses may have a different mechanistic basis.
Department of Physiology, McGill University, Montreal, QC, Canada.
Ryoji
R
Ide
Department of Physiology, McGill University, Montreal, QC, Canada.
Satoko Tomita
ST
Ide
Department of Physiology, McGill University, Montreal, QC, Canada. Electronic address: jacopo.mortola@mcgill.ca.
Jacopo P
JP
Mortola
D000818:Animals; D001831:Body Temperature / Q000502:physiology; D002245:Carbon Dioxide / Q000378:metabolism; D002642:Chick Embryo; D003080:Cold Temperature; D006935:Hypercapnia / Q000378:metabolism; D007035:Hypothermia / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D010507:Periodicity; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D012119:Respiration; D059246:Tachypnea / Q000378:metabolism
D002245:Carbon Dioxide; D010100:Oxygen
Breathing frequency; Breathing pattern generator; Control of breathing; Hypothermia; Respiratory arrest; Respiratory rhythm