Cold preservation with hyperbranched polyglycerol-based solution improves kidney functional recovery with less injury at reperfusion in rats.
Minimizing donor organ injury during cold preservation (including cold perfusion and storage) is the first step to prevent transplant failure. We recently reported the advantages of hyperbranched polyglycerol (HPG) as a novel substitute for hydroxyethyl starch in UW solution for both cold heart preservation and cold kidney perfusion. This study evaluated the functional recovery of the kidney at reperfusion after cold preservation with HPG solution. The impact of HPG solution compared to conventional UW and HTK solutions on tissue weight and cell survival at 4°C was examined using rat kidney tissues and cultured human umbilical vein endothelial cells (HUVECs), respectively. The kidney protection by HPG solution was tested in a rat model of cold kidney ischemia-reperfusion injury, and was evaluated by histology and kidney function. Here, we showed that preservation with HPG solution prevented cell death in cultured HUVECs and edema formation in kidney tissues at 4°C similar to UW solution, whereas HTK solution was less effective. In rat model of cold ischemia-reperfusion injury, the kidneys perfused and subsequently stored 1-hour with cold HPG solution showed less leukocyte infiltration, less tubular damage and better kidney function (lower levels of serum creatinine and blood urea nitrogen) at 48 h of reperfusion than those treated with UW or HTK solution. In conclusion, our data show the superiority of HPG solution to UW or HTK solution in the cold perfusion and storage of rat kidneys, suggesting that the HPG solution may be a promising candidate for improved donor kidney preservation prior to transplantation.
Department of Urology, Chengdu Military General HospitalChengdu, Sichuan, China; Department of Urologic Sciences, University of British ColumbiaVancouver, BC, Canada.
Shadan
S
Li
Department of Urologic Sciences, University of British ColumbiaVancouver, BC, Canada; Department of Critical Care Medicine, General Hospital of Tianjin Medical UniversityTianjin, China.
Bin
B
Liu
Department of Urologic Sciences, University of British Columbia Vancouver, BC, Canada.
Qiunong
Q
Guan
Centre for Blood Research, and The Department of Pathology and Laboratory Medicine, University of British Columbia Vancouver BC, Canada.
Irina
I
Chafeeva
Centre for Blood Research, and The Department of Pathology and Laboratory Medicine, University of British Columbia VancouverBC, Canada; Department of Chemistry, University of British ColumbiaVancouver, BC, Canada.
Donald E
DE
Brooks
Department of Urologic Sciences, University of British Columbia Vancouver, BC, Canada.
Christopher Yc
CY
Nguan
Centre for Blood Research, and The Department of Pathology and Laboratory Medicine, University of British Columbia VancouverBC, Canada; Department of Chemistry, University of British ColumbiaVancouver, BC, Canada.
Jayachandran N
JN
Kizhakkedathu
Department of Urologic Sciences, University of British Columbia Vancouver, BC, Canada.
Caigan
C
Du
HPG polymer; HTK; UW; cold preservation; donor kidney; rodent model; transplantation