Mate-pair editing: a perspective to double mate-pair sequencing coverage.
In this report, I am proposing a hypothetical approach that can enable sequencing of four short reads from the same insert using Illumina next-generation sequencing. The methodology is similar to that used in mate-pair sequencing except that it involves two circularization steps and the sequencing slide should have four different oligonucleotides.
School of Applied Systems Biology, La Trobe University, Bundoora, VIC, 3083, Australia. 17819359@students.latrobe.edu.au.
Abdulqader
http://orcid.org/0000-0002-2712-3698
A
Jighly
Mate-pair library; Next-generation sequencing