In vitro evaluation of antifilarial effect ofAzadirachta indicaleaves extract in different solvents on the microfilariae ofSetaria cervi.
Polar solvent extracts ofAzadirachta indicaleaves were investigated to evaluate in vitro anthelmintic activity on the microfilariae ofSetaria cervi. Four extracts ofA. indicaleaves were prepared with different solvents in increasing order of polarity (diethyl ether < chloroform < ethanol < methanol). Stock solution of the extract ofA. indicaleaves was prepared in Ringer's solution at the concentrations of 50, 100 and 200 μg/ml and used against microfilariae in three replicates. Ringer's solution was used as experimental control. Mortality rates of microfilariae were recorded for each concentration after 45, 90 and 135 min, respectively. All the solvent extracts show significant anthelmintic activity at the concentration of 200 μg/ml after 135 min of incubation. Highest mortality rate of microfilariae was observed in methanol and ethanol extracts throughout the incubation period compared to chloroform and diethyl ether extracts which showed low mortality rates. Dose dependent effect of ethanol and chloroform showed highest and lowest ranking when analyzed by regression values and correlation of regression. Solvents extracts ofA. indicaleaves showed anthelmintic effect and therefore may be used as phytopharmacological agents in future.
Section of Parasitology, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh India.
Sharba
0000-0002-2306-6921
S
Kausar
Azadirachta indica; Extracts; Microfilariae; Polar solvents; Setaria cervi