Quantitative analysis of adsorption and desorption behavior of individual cellulase components during the hydrolysis of lignocellulosic biomass with the addition of lysozyme.
The effect of non-catalytic protein addition on the adsorption/desorption behavior of individual cellulase components on/from substrates during the hydrolysis of microcrystalline cellulose and steam exploded sugarcane bagasse (SEB) were investigated. The addition of non-catalytic protein enhanced the enzymatic hydrolysis of SEB, but did not enhance the hydrolysis of microcrystalline cellulose. During the hydrolysis of SEB, adsorption of beta-glucosidase (BGL) was prevented in the presence of non-catalytic protein. Cellobiohydrolase I (CBH I) and endoglucanase I (EG I) desorbed from the substrate after temporary adsorption in the presence of non-catalytic protein during SEB hydrolysis. This suggested that reduction of the non-specific adsorption of cellulase components, CBH I, EG I, and BGL, on lignin in SEB led to the improving of enzymatic hydrolysis.
JGC Corporation, 2205, Narita-cho, Oarai-machi, Higashiibaraki-gun, Ibaraki Pref. 311-1313, Japan.
Yoshiko
Y
Toyosawa
JGC Corporation, 2205, Narita-cho, Oarai-machi, Higashiibaraki-gun, Ibaraki Pref. 311-1313, Japan.
Makoto
M
Ikeo
JGC Corporation, 2205, Narita-cho, Oarai-machi, Higashiibaraki-gun, Ibaraki Pref. 311-1313, Japan.
Daisuke
D
Taneda
JGC Corporation, 2205, Narita-cho, Oarai-machi, Higashiibaraki-gun, Ibaraki Pref. 311-1313, Japan. Electronic address: okino.shohei@jgc.com.
Shohei
S
Okino
D000327:Adsorption; D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry; D043366:Cellulose 1,4-beta-Cellobiosidase; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D009113:Muramidase
D002482:Cellulose; D008031:Lignin; D009113:Muramidase; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase
Enzyme adsorption/desorption; Lignocellulosic bioethanol; Lysozyme; Non-specific adsorption; Sugarcane bagasse