High-performance liquid chromatographic determination of multi-mycotoxin in cereals and bean foodstuffs using interference-removal solid-phase extraction combined with optimized dispersive liquid-liquid microextraction.
A novel pre-treatment was proposed for the simultaneous determination of aflatoxins, ochratoxin A and zearalenone in foodstuffs using high-performance liquid chromatography with fluorescence detection. The analytical procedure was based on a first step using a quick, easy, cheap, effective, rugged, and safe based extraction procedure, followed by salting out and purification with a C18solid-phase extraction column as interference removal clean-up. Subsequently, collected supernatant was subjected to dispersive liquid-liquid microextraction. Response surface methodology based on central composite design was employed to optimize conditions in the microextraction procedure. Under the optimum conditions, satisfactory analytical performance with recoveries ranging from 63.22 to 107.6% were achieved in different types of cereals and beans, as well as desirable precisions (0.81-8.13%). Limits of detections and quantifications for these six mycotoxins ranging from 0.03 to 13 μg/kg and 0.22 to 44 μg/kg, respectively, were obtained. Finally, the established method was successfully validated by four certified reference materials (P = 0.897 > 0.05) and applied to 79 samples from local markets.
College of Chemical Engineering, Zhejiang University of Technology, Zhejiang, China.|Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Zhejiang, China.
Jian
J
Zhou
Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Zhejiang, China.
Jiao-Jiao
http://orcid.org/0000-0001-6697-5968
JJ
Xu
Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Zhejiang, China.
Bai-Fen
BF
Huang
Lab of Physicochemical Research, Department of Physicochemical & Toxicology, Zhejiang Provincial Center for Disease Control and Prevention, Zhejiang, China.
Zeng-Xuan
ZX
Cai
College of Chemical Engineering, Zhejiang University of Technology, Zhejiang, China.|National Center for Food Safety Risk Assessment Application Technology Cooperation Center, Yangtze Delta Region Institute of Tsinghua University, Zhejiang, China.
Yi-Ping
YP
Ren
D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D059627:Liquid Phase Microextraction; D009183:Mycotoxins / Q000032:analysis*; D052616:Solid Phase Extraction
D009183:Mycotoxins
central composite design; dispersive liquid-liquid microextraction; multi-mycotoxin; response surface methodology; solid-phase extraction