Phylogenetic analysis of env gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan.
To understand how the latest dominant bovine leukemia virus (BLV) strains were introduced and spread in the Miyazaki prefecture, we collected blood samples from 3 geographic areas (north, central and south) and carried out sequence analysis of the BLV env gene. Two genotypes, genotype I, and III, were identified and the majority of the strains belonged to genotype I (71/74). To clarify a route of BLV introduction, we divided the strains into 20 subgenotypes based on their nucleotide sequences and performed phylogenetic analysis. Our study indicated that common BLV strains were comparatively evenly distributed even in the area, where the farmers have not introduced cattle from other areas and the cattle have limited exposure to BLV infection in grazing fields.
Laboratory of Animal Infectious Disease and Prevention, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.|Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Al Qalyubia Governorate 13511, Egypt.
Marawan A
MA
Marawan
Organization for Promotion of Tenure Track, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Hirohisa
H
Mekata
Laboratory of Animal Infectious Disease and Prevention, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Takumi
T
Hayashi
Laboratory of Animal Infectious Disease and Prevention, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Satoshi
S
Sekiguchi
Project for Zoonoses Education and Research, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Yumi
Y
Kirino
Division of International Cooperation and Education, Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Yoichiro
Y
Horii
Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Al Qalyubia Governorate 13511, Egypt.
Abdel-Moneim M
AM
Moustafa
Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Al Qalyubia Governorate 13511, Egypt.
Faysal K
FK
Arnaout
Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Al Qalyubia Governorate 13511, Egypt.
El Sayed M
ESM
Galila
Laboratory of Animal Infectious Disease and Prevention, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.|Division of International Cooperation and Education, Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
Junzo
J
Norimine
D000818:Animals; D002417:Cattle; D004279:DNA, Viral; D016583:Enzootic Bovine Leukosis / Q000821:virology*; D015752:Genes, env; D007564:Japan; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004279:DNA, Viral
BLV; genotype; phylogenetic analysis