Insulin-like growth factor 1 gene polymorphism in women with breast cancer.
Breast cancer is a disease of unknown etiology; however, the major risk factors are genetic alterations. Studies have demonstrated an association between insulin-like growth factor 1 (IGF-1) gene polymorphism and cell proliferation and reduced apoptosis, in addition to its role in breast cancer growth and aggressiveness. Two polymorphic variants of the IGF-1 gene are highlighted in association with breast cancer, rs6220 and rs7136446, although controversy exists as to this relationship. The current study included 137 women (68 breast cancer cases and 69 controls without breast cancer) who had 3 ml of peripheral blood drawn for the study of genomic DNA extracted from leukocytes using the genotyping technique by real-time polymerase chain reaction. The CC genotype (rs7136446) was present in 4 women (5.9%) from the case group and in 2 (3.0%) women from the control group (p = 0.67), while the GG genotype (rs6220) occurred in 8 (11.5%) women from the case group and in 5 (7.2%) women from the control group (p = 0.75). No statistically significant difference was observed between the CC genotype of variant rs7136446 in premenopausal case and control women (p = 0.31), thus as there was also no significant difference between case and control postmenopausal women (p = 1.00). Concerning the GG genotype of rs6220, it occurred in 6 (14.2%) premenopausal case and 4 (8%) control women (p = 0.71) and no difference was found in postmenopausal women (p = 1.00). In the current study, IGF-1 gene polymorphism of SNP variants rs6220 and rs7136446 had no statistically significant association with breast cancer, both in premenopausal and postmenopausal women.
Postgraduate Program of Health and Sciences, Federal University of Piaui, 2280 Frei Serafim Avenue, Teresina, Piauí, 64049-550, Brazil.|Postgraduate Program of the Northeast Network of Biotechnology (RENORBIO), Federal University of Piauí, Teresina, 64049-550, Brazil.
Danylo Rafhael
DR
Costa-Silva
Postgraduate Program of Health and Sciences, Federal University of Piaui, 2280 Frei Serafim Avenue, Teresina, Piauí, 64049-550, Brazil.|Postgraduate Program of the Northeast Network of Biotechnology (RENORBIO), Federal University of Piauí, Teresina, 64049-550, Brazil.
Maria
M
da Conceição Barros-Oliveira
Postgraduate Program of Health and Sciences, Federal University of Piaui, 2280 Frei Serafim Avenue, Teresina, Piauí, 64049-550, Brazil.
Rafael Soares
RS
Borges
Postgraduate Program of Health and Sciences, Federal University of Piaui, 2280 Frei Serafim Avenue, Teresina, Piauí, 64049-550, Brazil.
Larysse Maira
LM
Campos-Verdes
Postgraduate Program of Health and Sciences, Federal University of Piaui, 2280 Frei Serafim Avenue, Teresina, Piauí, 64049-550, Brazil.
João Paulo
JP
da Silva-Sampaio
Postgraduate Program of the Northeast Network of Biotechnology (RENORBIO), Federal University of Piauí, Teresina, 64049-550, Brazil.
Carla Solange
CS
Escorcio-Dourado
Postgraduate Program of the Northeast Network of Biotechnology (RENORBIO), Federal University of Piauí, Teresina, 64049-550, Brazil.
Luana Mota
LM
Martins
Department of Statistics, University of São Paulo, São Paulo, 03178-200, Brazil.
Airlane Pereira
AP
Alencar
Department of Gynecology, University of São Paulo, São Paulo, 03178-200, Brazil.
Edmund Chada
EC
Baracat
Department of Molecular Biology, Natan Portella Hospital, Federal University of Piaui, Teresina, 64001-450, Brazil.
Vladimir Costa
VC
Silva
Postgraduate Program of Health and Sciences, Federal University of Piaui, 2280 Frei Serafim Avenue, Teresina, Piauí, 64049-550, Brazil. beneditoborges@globo.com.|Postgraduate Program of the Northeast Network of Biotechnology (RENORBIO), Federal University of Piauí, Teresina, 64049-550, Brazil. beneditoborges@globo.com.
Benedito Borges
BB
da Silva
D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
D007334:Insulin-Like Growth Factor I
Breast cancer; Genetic polymorphism; IGF-1; Status menopausal; qPCR