Arthropod and oligochaete assemblages from grasslands of the southern Kenai Peninsula, Alaska.
BACKGROUND
By the end of this century, the potential climate-biome of the southern Kenai Peninsula is forecasted to change from transitional boreal forest to prairie and grasslands, a scenario that may already be playing out in the Caribou Hills region. Here, spruce (Picea × lutzii Little [glauca×sitchensis]) forests were heavily thinned by an outbreak of the spruce bark beetle (Dendroctonus rufipennis(Kirby, 1837)) and replaced by the native but invasive grass species,Calamagrostis canadensis(Michx.) P. Beauv. As part of a project designed to delimit and characterize potentially expanding grasslands in this region, we sought to characterize the arthropod and earthworm communities of these grasslands. We also used this sampling effort as a trial of applying high-throughput sequencing metabarcoding methods to a real-world inventory of terrestrial arthropods.


CONCLUSIONS
We documented 131 occurrences of 67 native arthropod species at ten sites, characterizing the arthropod fauna of these grasslands as being dominated by Hemiptera (60% of total reads) and Diptera (38% of total reads). We found a single exotic earthworm species,Dendrobaena octaedra(Savigny, 1826), at 30% of sites and one unidentified enchytraeid at a single site. The utility of high-throughput sequencing metabarcoding as a tool for bioassessment of terrestrial arthropod assemblages was confirmed.
U.S. Fish & Wildlife Service, Kenai National Wildlife Refuge, Soldotna, Alaska, United States of America.
Matthew L
ML
Bowser
U.S. Fish & Wildlife Service, Kenai National Wildlife Refuge, Soldotna, Alaska, United States of America.
John M
JM
Morton
Research and Testing Laboratory, Lubbock, Texas, United States of America.
John Delton
JD
Hanson
U.S. Fish & Wildlife Service, Kenai National Wildlife Refuge, Soldotna, Alaska, United States of America.
Dawn R
DR
Magness
U.S. Fish & Wildlife Service, Kenai National Wildlife Refuge, Soldotna, Alaska, United States of America.
Mallory
M
Okuly
 Arthropoda ; Illumina MiSeq; exotic earthworms; metagenomics