Entrapment of DyP-type peroxidase from Pseudomonas fluorescens Pf-5 into Ca-alginate magnetic beads.
The aim of this study was to investigate the optimal conditions for the immobilization and stabilization of DyP1B dye decolorizing peroxidases type B (DyP1B) from Pseudomonas fluorescens Pf-5 immobilized in Ca-alginate ferromagnetic beads. The immobilized DyP1B was used in the degradation of the Reactive Blue 5 (RB5) synthetic dye. The enzyme was successfully entrapped in a Ca-alginate matrix and showed an encapsulation efficiency of 94%. The concentration of DyP1B (0.8 mg mL-1), 2% of alginate and magnetite (10.0 mg mL-1) was optimal for immobilization. The immobilized DyP1B showed optimum activity at pH 7.0 and 40 °C compared with pH 5.5 and 30 °C for free peroxidase. Reusability studies showed that after five cycles, the immobilized DyP1B system retained more than 58% of its initial activity. The immobilized DyP1B was able to decolorize RB5 at concentrations of 0.1, 0.05, and 0.01% (w v-1) with efficiency rates of about 20, 29, and 45%, respectively. The immobilization of DyP1B in alginate beads with the addition of Fe3O4increased its catalytic and applicative potential.
Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland.
Agata
http://orcid.org/0000-0002-0660-1224
A
Wasak
Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland.
Radosław
R
Drozd
Department of Organic and Physical Chemistry, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin, Poland.
Łukasz
Ł
Struk
Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland.
Bartłomiej
B
Grygorcewicz
D000464:Alginates / Q000737:chemistry*; D000880:Anthraquinones / Q000302:isolation & purification*; D004396:Coloring Agents / Q000302:isolation & purification*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D059346:Magnets / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000201:enzymology*
D000464:Alginates; D000880:Anthraquinones; D004396:Coloring Agents; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; C544286:Reactive Blue 5; D020723:Glucuronic Acid; D009195:Peroxidase; D052203:Ferrosoferric Oxide
Ca-alginate beads; DyP-type peroxidases; decolorization of Reactive Blue 5; immobilization