Mesenchymal stromal cells enhance the suppressive effects ofmyeloid-derived suppressor cells of multiple myeloma.
Both mesenchymal stromal cells (MSCs) and myeloid-derived suppressor cells (MDSCs) have immunosuppressive properties, and their presence may confer a worse prognosis upon cancer patients. However, whether MSCs can enhance the immunosuppressive effects of MDSCs in MM remains unknown. We evaluated the influence of MSCs on MDSCs growth, apoptosis, and functions. Our results show that MSCs promote proliferation and inhibit apoptosis in MDSCs. Additionally, MSCs enhance the ability of MDSCs by inhibiting T-cell proliferation and IFN-γ production. Furthermore, both the mRNA and protein levels of Arg1 and NOS2 were upregulated in MDSCs. These results suggest that MSCs may exert immunomodulatory effects on MDSCs by upregulating Arg1 and NOS2.
a Thoracic Surgery , The Second Affiliated Hospital of Guilin Medical University , Guilin, Guangxi , China.
Yinhui
Y
Xu
b Department of Hematology, Key Laboratory of Cancer Prevention and Therapy , Cancer Hospital of Tianjin, Tianjin Medical University , Tianjin , China.
Xiaoying
X
Zhang
c Department of Laboratory Medicine , The Second Affiliated Hospital of Guilin Medical University , Guilin, Guangxi , China.
Hongbo
H
Liu
b Department of Hematology, Key Laboratory of Cancer Prevention and Therapy , Cancer Hospital of Tianjin, Tianjin Medical University , Tianjin , China.
Pan
P
Zhao
b Department of Hematology, Key Laboratory of Cancer Prevention and Therapy , Cancer Hospital of Tianjin, Tianjin Medical University , Tianjin , China.
Yafang
Y
Chen
d Department of Cancer Research , University of South China , Hengyang , China.
Yimin
Y
Luo
e Department of Neurology , the Fourth Central Hospital , Tianjin , China.
Zhiqing
Z
Zhang
b Department of Hematology, Key Laboratory of Cancer Prevention and Therapy , Cancer Hospital of Tianjin, Tianjin Medical University , Tianjin , China.
Xiaofang
X
Wang
D017209:Apoptosis / Q000276:immunology*; D001119:Arginase / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D022423:Myeloid Cells / Q000276:immunology* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D007371:Interferon-gamma; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D001119:Arginase
Arg1; MDSCs; MSCs; NOS2; multiple myeloma