Validation of Multiresidue Method for Analysis of 31 Pesticides in Rice Using Gas Chromatography-Tandem Mass Spectrometry.
Five modified QuEChERS were tested for the multiresidue analysis of 31 pesticides in rice. Rice was spiked with mixturesolution of pesticides at 10 ng/g. Method selection was based on the LODs (sensitivity) and recovery tests (accuracy) of the pesticides. Analysis was done in GC-tandem MS in multiple reaction monitoring mode with a total run time of approximately 37 min. The selected method was validated after spiking rice at 20 and 100 ng/g in rice. The performance characteristics of the method impacted for all selected pesticides were acceptable according to the guidelines for method validation (recovery of 70-120% with an RSD of <20% and r2value of ≥0.99). For rice, matrix effect on the signals of the compounds was corrected by using matrix-matched calibration standards. The LOQs met the requirements of the maximum residue limits for pesticides in rice. The developed method allowed for the simultaneous determination and confirmation of a large number of different groups of pesticides and was fast, simple, inexpensive, and useful for the routine analysis of rice.
Bidhan Chandra Krishi Viswavidyalaya, Faculty of Agriculture, Department of Agricultural Chemicals, Mohanpur 741252, Nadia, West Bengal, India.
Rahul
R
Mondal
Bidhan Chandra Krishi Viswavidyalaya, Faculty of Agriculture, Department of Agricultural Chemicals, Mohanpur 741252, Nadia, West Bengal, India.
Ramen Kumar
RK
Kole
Bidhan Chandra Krishi Viswavidyalaya, Faculty of Agriculture, Department of Agricultural Chemicals, Mohanpur 741252, Nadia, West Bengal, India.
Anjan
A
Bhattacharyya