Synthesis, crystallization and preliminary crystallographic analysis of a 52-nucleotide DNA/2'-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with a substrate.
A 52-nucleotide DNA/2'-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with its substrate was synthesized, purified and crystallized by the hanging-drop method using 0.8 M sodium potassium tartrate as a precipitant. A data set to 1.21 Å resolution was collected from a monocrystal at 100 K using synchrotron radiation on a beamline BL14.1 at BESSY. The crystal belonged to the P21group with unit-cell a = 49.42, b = 24.69, c = 50.23, β = 118.48.
a Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences , Department of Bioorganic Chemistry , Łódź , Poland.
Rafał
R
Dolot
a Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences , Department of Bioorganic Chemistry , Łódź , Poland.
Milena
M
Sobczak
a Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences , Department of Bioorganic Chemistry , Łódź , Poland.
Barbara
B
Mikołajczyk
a Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences , Department of Bioorganic Chemistry , Łódź , Poland.
Barbara
B
Nawrot
D001483:Base Sequence; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D003460:Crystallization; D021881:DNA, Catalytic / Q000378:metabolism*; D009711:Nucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics
D021881:DNA, Catalytic; D009711:Nucleotides; D012313:RNA
10–23; Chimeric oligonucleotide; DNAzyme; deoxyribozyme; nucleic acid crystallization