Alpha-tocopherol-dependent salt tolerance is more related with auxin synthesis rather than enhancement antioxidant defense in soybean roots.
In this paper, we describe the alleviated effects of alpha-tocopherol (α-T) on oxidative damage and its possible role as a signal transmitter in plants during salt stress. The results show that exogenously applied α-T under salt stress increased root length and weight, but reduced hydrogen peroxide (H2O2), superoxide anion radical (O2.-) and malondialdehyde (MDA) content in soybean roots. The proline content was reduced by α-T treatment. Interestingly, endogenous auxin (IAA) level was significantly increased after α-T application as compared to salt stress alone. Moreover, α-T reduced significantly superoxide dismutase (SOD) enzyme and isoenzyme activity but upregulated peroxidase (POX) 2, 3 and glutathione-s-transferase (GST) 1, 3 isoenzyme expression. However, ascorbate peroxidase (APX) enzyme activity was not affected at all. Consequently, the results show that α-T serves as a signal molecule under salinity from leaves to roots by increasing remarkably endogenous IAA levels and increasing partially antioxidant activity in roots.
Department of Biology, Faculty of Art and Science, Sinop University , Sinop , Turkey.
Seda
S
Sereflioglu
Department of Biology, Faculty of Art and Science, Sinop University , Sinop , Turkey.
Burcu Seckin
BS
Dinler
Department of Biology, Faculty of Art and Science, Sinop University , Sinop , Turkey.
Eda
E
Tasci
D000975:Antioxidants / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D055049:Salt Tolerance / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology*; D013025:Glycine max / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors; D014815:Vitamins / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000494:pharmacology*
D000975:Antioxidants; D007210:Indoleacetic Acids; D007527:Isoenzymes; D010940:Plant Proteins; D014815:Vitamins; D013481:Superoxides; D012965:Sodium Chloride; D008315:Malondialdehyde; D011392:Proline; D006861:Hydrogen Peroxide; D010544:Peroxidases; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D024502:alpha-Tocopherol
Alpha-tocopherol; IAA; antioxidant; salinity; soybean