Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells.
Infection with all serotypes of dengue virus (DV) results in augmented antigen presentation by MHC class I molecules. However, the upregulation of immunoproteasome subunits only results from infection with two serotypes. This study aims to elucidate changes in the expression of immunoproteasome subunits resulting from infection with DV, particularly DV serotype 2 (DV2).
HepG2 cells were grown in various culture milieu. Total cellular RNA and proteins were extracted and quantified.
Results demonstrated sequestration of immunoproteasome subunits LMP2 and LMP7 in DV2-infected cells.
This study provides insights into the mechanisms underlying immune evasion by DV.
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|Drug Design and Development Research Group, University of Malaya, Kuala Lumpur, Malaysia.
Chye Sheng
CS
Gan
Institute of Graduate Studies, University of Malaya, Kuala Lumpur, Malaysia.
Pei Jean
PJ
Lim
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Muhammad Fazril Mohamad
MF
Razif
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|Drug Design and Development Research Group, University of Malaya, Kuala Lumpur, Malaysia.
Rohana
R
Yusof
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|Drug Design and Development Research Group, University of Malaya, Kuala Lumpur, Malaysia.
Shatrah
S
Othman
D003716:Dengue Virus / Q000145:classification / Q000378:metabolism*; D005786:Gene Expression Regulation; D056945:Hep G2 Cells; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D021122:Protein Subunits; D065288:Serogroup
D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex