ZnO nanorods array based field-effect transistor biosensor for phosphate detection.
A promising field-effect transistor (FET) biosensor has been fabricated based on pyruvate oxidase (PyO) functionalized ZnO nanorods (ZnO NRs) array grown on seeded SiO2/Si substrate. The direct and vertically grown ZnO NRs on the seeded SiO2/Si substrate offers high surface area for enhanced PyO immobilization, which further helps to detect phosphate with higher specificity. Under optimum conditions, the fabricated FET biosensor provided a convenient method for phosphate detection with high sensitivity (80.57μAmM-1cm-2) in a wide-linear range (0.1µM-7.0mM). Additionally, it also showed very low effect of electroactive species, stability and good reproducibility. Encouraging results suggest that this approach presents a promising method to be used for field measurements to detect phosphate.
School of Semiconductor and Chemical Engineering, Nanomaterials Processing Research Center, Chonbuk National University, 567 Baekjedaero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
Rafiq
R
Ahmad
School of Semiconductor and Chemical Engineering, Nanomaterials Processing Research Center, Chonbuk National University, 567 Baekjedaero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
Min-Sang
MS
Ahn
School of Semiconductor and Chemical Engineering, Nanomaterials Processing Research Center, Chonbuk National University, 567 Baekjedaero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea. Electronic address: ybhahn@chonbuk.ac.kr.
Yoon-Bong
YB
Hahn
D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D010710:Phosphates / Q000032:analysis*; D011771:Pyruvate Oxidase / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry*
D010710:Phosphates; D012822:Silicon Dioxide; D011771:Pyruvate Oxidase; D015034:Zinc Oxide; D012825:Silicon
Field-effect transistor; High sensitivity; Phosphate biosensor; Pyruvate oxidase; ZnO nanorods