Macrophage Kdm6b controls the pro-fibrotic transcriptome signature of foam cells.
In order to identify regulators of foam cells, we studied the H3K27 demethylase Kdm6b (also known as Jmjd3), a known regulator of macrophages, in controlling the transcriptional profile of foam cells.



Foam cells from Kdm6b-deleted or Kdm6b wild-type mice were isolated and used for RNA-sequencing analysis.



Pathway analysis revealed that pro-fibrotic pathways were strongly suppressed in Kdm6b-deleted foam cells. Analysis of published datasets showed that foam cell formation induces these pro-fibrotic characteristics. Overlay of both datasets indicated that fibrotic genes which are induced upon foam cell formation, are reduced in the absence of Kdm6b. These data suggest that foam cell formation induces a pro-fibrotic gene signature in a Kdm6b-dependent manner.



We identified Kdm6b as a novel regulator of the pro-fibrotic signature of peritoneal foam cells.
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam, The Netherlands.
Annette E
AE
Neele
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam, The Netherlands.
Koen Hm
KH
Prange
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam, The Netherlands.
Marten A
MA
Hoeksema
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam, The Netherlands.
Saskia
S
van der Velden
Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University, Frankfurt, Germany.
Tina
T
Lucas
Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University, Frankfurt, Germany.
Stefanie
S
Dimmeler
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam, The Netherlands.|Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University of Munich, Munich, Germany.
Esther
E
Lutgens
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam, The Netherlands.
Jan
J
Van den Bossche
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam, The Netherlands.|Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University of Munich, Munich, Germany.
Menno Pj
MP
de Winther
D000818:Animals; D005355:Fibrosis; D005487:Foam Cells / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D053263:Gene Regulatory Networks; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D010529:Peritoneal Cavity / Q000166:cytology; D017423:Sequence Analysis, RNA / Q000379:methods*; D015398:Signal Transduction
D056484:Jumonji Domain-Containing Histone Demethylases; C523761:Kdm6b protein, mouse
Jmjd3; JmjdC; Kdm6b; epigenetics; fibrosis; histone H3K27me3; histone demethylase; histone modification; macrophage; matrix biology