In vitro preparation and assessment of radical reducing peptide from Octopus aegina using digestive proteases.
Antioxidant peptides protect biological macromolecules against radical damages. The use of these peptides was evaluated using free radicals scavenging assays [2,2-diphenyl-1 picrylhydrazyl (DPPH) and hydroxyl] with the help of UV-visible and electron spin resonance (ESR) spectroscopy methods. The Octopus aegina mantle protein were tested upon hydrolysis using gastrointestinal enzymes up to 12 h, where pepsin hydrolysate exhibited superior properties (DPPH: 44.39±0.67% and hydroxyl: 38.84±1.07%) compared with trypsin and α-chymotrypsin. Consequently, the antioxidant activity of the purified hydrolysate increased on a successive purification, and the peptide sequence was determined to be 368.9 Da with Gly-Glu-Tyr amino acids. Tripeptide exerted free radical scavenging efficiency in DNA damage, lipid peroxidation and cellular destruction (MCF7 cells) under stress condition. The results obtained with octopus antioxidant peptide suggested its role as an adjunct in food and pharmaceutical industries.
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai 603 203, Tamilnadu, India.
Sekar
S
Sudhakar
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai 603 203, Tamilnadu, India. Electronic address: nazeer.ra@ktr.srmuniv.ac.in.
Rasool Abdul
RA
Nazeer
D000595:Amino Acid Sequence; D000818:Animals; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D015227:Lipid Peroxidation / Q000187:drug effects; D061986:MCF-7 Cells; D049831:Octopodiformes; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D059748:Proteolysis
D016166:Free Radical Scavengers; D010455:Peptides; D010447:Peptide Hydrolases
Antioxidant activity; Electrospray ionization mass spectrometry; Lipid oxidation; MCF7 cells; Octopus aegina; Protein hydrolysate