Hydroxy methoxy benzaldehyde from Sesbania grandilfora inhibits the advanced glycation end products (AGEs)-mediated fibrillation in hemoglobin.
The present study was aimed to identify the active anti-glycation constituent from the leaves of Sesbania grandiflora. Characterization of the active constituent resulted in the identification of hydroxy methoxy benzaldehyde (HMB). The potential of HMB as anti-glycation lead was analyzed by fluorescence spectroscopy, fluorescence microscopy, scanning electron microscopy (SEM) and molecular interaction studies. Our results suggested that HMB inhibited formation of early (HbA1c) and advanced glycation end products (AGEs). The amyloid-like fibrillation in hemoglobin was also inhibited by HMB. SEM images indicated the protective effect against the formation of acanthocytes. Molecular docking studies showed that HMB was interacting with hemoglobin through hydrogen bonds with Arg141, Tyr140, and Thr137. Our findings suggest that HMB could be a better anti-glycation lead molecule towards novel AGEs inhibitors.
a Molecular Biophysics Laboratory, School of Chemical and Biotechnology , SASTRA University , Thanjavur , Tamilnadu 613401 , India.
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Prasanna
b NMR Laboratory, School of Chemical and Biotechnology , SASTRA University , Thanjavur , Tamilnadu 613401 , India.
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Hari
a Molecular Biophysics Laboratory, School of Chemical and Biotechnology , SASTRA University , Thanjavur , Tamilnadu 613401 , India.
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0000-0002-9061-1571
NT
Saraswathi
D001547:Benzaldehydes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000188:drug therapy* / Q000473:pathology; D006442:Glycated Hemoglobin / Q000037:antagonists & inhibitors / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors / Q000737:chemistry; D006031:Glycosylation / Q000187:drug effects; D006454:Hemoglobins / Q000037:antagonists & inhibitors / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding / Q000187:drug effects; D062105:Molecular Docking Simulation; D018515:Plant Leaves / Q000737:chemistry; D049931:Sesbania / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D001547:Benzaldehydes; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006454:Hemoglobins
HbA1c; advanced glycation end products; amyloid fibrils; fluorescence microscopy; glycation; hemoglobin; red blood cells