A Brief Review of Bioinformatics Tools for Glycosylation Analysis by Mass Spectrometry.
The purpose of this review is to provide updated information regarding bioinformatic software for the use in the characterization of glycosylated structures since 2013. A comprehensive review by Woodinet al.Analyst138: 2793-2803, 2013 (ref. 1) described two main approaches that are introduced for starting researchers in this area; analysis of released glycans and the identification of glycopeptide in enzymatic digests, respectively. Complementary to that report, this review focuses on mass spectrometry related bioinformatics tools for the characterization of N-linked and O-linked glycopeptides. Specifically, it also provides information regarding automated tools that can be used for glycan profiling using mass spectrometry.
Department of Chemistry, National Taiwan Normal University; Mithra Biotechnology Inc.
Pei-Lun
PL
Tsai
Department of Chemistry, National Taiwan Normal University.
Sung-Fang
SF
Chen
glycan; glycopeptide; glycoproteomics; glycosylation; mass spectrometry