Evaluation of a real-time impedance analysis platform on fungal infection.
End-point assays of in vitro cell proliferation and death have been employed to study the mechanisms of fungal pathogenesis and have shown the responses of host cells at individual time points. A new cell analysis technology has been developed that allows for the continuous measurement and quantification of cell activities, thus enabling the dynamic assessment of electrical impedance when various pathogens are cultured in vitro. In this study, this system was evaluated to determine the response of the cell line RAW264.7 to infection by several clinically relevant fungi in vitro, including Aspergillus fumigatus, Candida albicans, and melanized and albino mutant strains of Fonsecaea monophora. The results showed that infection resulted in rounding of the host cells with a loss of contact between individual cells and a decline in the electrical impedance of all test groups. However, changes in the electrical impedance were variable. Aspergillus fumigatus caused initial increases and later significant decreases in the electrical impedance, while for C. albicans and F. monophora, the effect was reduced. The melanized strain of F. monophora caused a faster change in the electrical impedance than the albino strain. Our data proved that this system can be used as an efficient tool for monitoring cellular responses to fungal infection.
Guangdong Provincial Institute of Public Health, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Jiufeng
J
Sun
Guangdong Provincial Institute of Public Health, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
Dan
D
Ning
Department of Dermatology, Sun Yat Sen Memorial Hospital, Sun Yat Sen University, Guangzhou, China.
Wenying
W
Cai
Key Laboratory for Repository and Application of Pathogenic Microbiology, Research Center for Pathogens Detection Technology of Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious Diseases, Guangzhou, China.
Huiqiong
H
Zhou
Key Laboratory for Repository and Application of Pathogenic Microbiology, Research Center for Pathogens Detection Technology of Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious Diseases, Guangzhou, China.
Huan
H
Zhang
Key Laboratory for Repository and Application of Pathogenic Microbiology, Research Center for Pathogens Detection Technology of Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious Diseases, Guangzhou, China.
Dawei
D
Guan
Key Laboratory for Repository and Application of Pathogenic Microbiology, Research Center for Pathogens Detection Technology of Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious Diseases, Guangzhou, China. Electronic address: wu_de68@cdcp.org.cn.
De
Wu
D000818:Animals; D001203:Ascomycota / Q000254:growth & development / Q000472:pathogenicity; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000472:pathogenicity; D002176:Candida albicans / Q000254:growth & development / Q000472:pathogenicity; D049109:Cell Proliferation; D017097:Electric Impedance; D005658:Fungi / Q000472:pathogenicity*; D051379:Mice; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D000067996:RAW 264.7 Cells / Q000382:microbiology
Albino mutant; Aspergillus fumigatus; Candida albicans; Fonsecaea monophora; Fungal infection; Pathogenesis; Real-time cell analysis