Antibodies against Apicomplexa protozoa and absence sarcocysts in heart tissues from horses in southern Brazil.
Sarcocystis spp., Neospora spp., and Toxoplasma gondii are Apicomplexa protozoa that can infect horses. This study aimed to investigate the occurrence of antibodies against Sarcocystis spp., Neospora spp., and T. gondii in horses slaughtered in southern Brazil. The presence of histological lesions, tissue cysts, and Sarcocystis spp. DNA in the hearts of these horses was also investigated. A total of 197 paired serum and heart samples were evaluated by serology and direct microscopic examination; 50 of these samples were subjected to histopathological and PCR analyses. Antibodies against at least one of the protozoa were detected in 146 (74.1%) of the serum samples. The frequencies of positive serology were: 36% (71/197) against Sarcocystis spp., 39.1% (77/197) against Neospora spp., and 47.2% (93/197) against T. gondii. No cysts, Sarcocystis spp. DNA, or histopathological lesions were observed in myocardial tissue samples. The frequencies of antibody seropositivity against Sarcocystis spp., Neospora spp., and T. gondii showed that horses are frequently infected by these parasites in southern Brazil. The absence of sarcocysts in horse tissues is compatible with their role as aberrant/accidental hosts in the life cycle of Sarcocystis spp..
Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva - DMVP, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Luiza Pires
LP
Portella
Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva - DMVP, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Gustavo Cauduro
GC
Cadore
Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva - DMVP, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Luis Antonio
LA
Sangioni
Setor de Indústria e Inspeção de Carnes, Departamento de Medicina Veterinária Preventiva - DMVP, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Luiz Fernando Vilani
LF
Pellegrini
Laboratório de Patologia Veterinária, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Rafael
R
Fighera
Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva - DMVP, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Fernanda
F
Ramos
Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva - DMVP, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Fernanda Silveira Flores
FS
Vogel
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D001938:Brazil; D003048:Coccidiosis / Q000662:veterinary; D006321:Heart / Q000469:parasitology*; D006734:Horse Diseases / Q000276:immunology / Q000469:parasitology*; D006736:Horses; D009206:Myocardium / Q000276:immunology; D018688:Neospora / Q000276:immunology*; D012522:Sarcocystis / Q000276:immunology*; D012523:Sarcocystosis / Q000662:veterinary; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology*
D000913:Antibodies, Protozoan