Rapid Characterization of the major chemical constituents from Polygoni Multiflori Caulis by liquid chromatography tandem mass spectrometry and comparative analysis with Polygoni Multiflori Radix.
Polygoni Multiflori Caulis is a traditional Chinese medicine that has been used for a long time to treat sleep disorders. However, the multiple chemical composition analysis has not been reported. In this study, a simple, rapid and effective ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established to characterize the components of Polygoni Multiflori Caulis. In addition, a chemical comparative analysis was performed with Polygoni Multiflori Radix, another traditional Chinese medicine from the same plant, through multivariate statistical analysis and semi-quantitative analysis to screen the difference in chemical ingredients between these two herbal medicines from same medicinal plant. A total of 33 peaks were detected within 25 min, and 28 of them were identified or tentatively characterized by comparing the retention time and mass spectrometry data. Based on the results, 12 characteristic components were screened out by multivariate statistical analysis, and their content change trends were compared by semi-quantitative analysis.
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, P. R. China.|Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang, P. R. China.|State Key Lab of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang, P. R. China.
Gui-Yang
GY
Wang
Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang, P. R. China.|State Key Lab of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang, P. R. China.
Jing
J
Shang
Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang, P. R. China.|State Key Lab of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang, P. R. China.
Yun
Y
Wu
Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang, P. R. China.|State Key Lab of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang, P. R. China.
Gang
G
Ding
Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang, P. R. China.|State Key Lab of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang, P. R. China.
Wei
W
Xiao
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D008516:Medicine, Chinese Traditional; D018517:Plant Roots / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D004365:Drugs, Chinese Herbal
Polygoni Multiflori Caulis; liquid chromatography tandem mass spectrometry; multivariate statistical analysis; rapid chemical identification