Effects of the culture media optimization on pectinase production by Enterobacter sp. PSTB-1.
In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO32.0 g/l, KCl 0.50 g/l, KH2PO41.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.
Department of Biotechnology, Sri Venkateswara University, Tirupati, 517 502, India.
M Purna Chandra
MP
Reddy
Department of Biotechnology, Sri Venkateswara University, Tirupati, 517 502, India. kvsarithasvu@gmail.com.|Department of Future Studies, SVU College of Sciences, Sri Venkateswara University, Tirupati, 517 502, A.P., India. kvsarithasvu@gmail.com.
K V
KV
Saritha
Central composite design (CCD) model; Enterobacter sp. PSTB-1; Mango fruit processing industrial waste (MIW) powder; Pectinase; Response Surface Methodology (RSM); Submerged fermentation