Molecular characterization ofMyxoboluscatmrigalae(Myxosporea: Myxobolidae) infecting the gill lamellae of carpCirrhinusmrigala(Hamilton).
The present study attempted sequencing the 18S rRNA gene ofMyxoboluscatmrigalaeinfecting the gill lamellae of carp,Cirrhinusmrigalaand compared its genetic homology and phylogenetic characteristics with 18S rRNA genes of otherMyxobolusspp. The infected fish had up to 3 small, creamy white plasmodia per gill filament with 30-50 spores each. The spore size was 17.90 ± 0.70 × 7.40 ± 0.40 μm. The sporoplasm contained two large nuclei of size 0.57 ± 0.09 μm and no iodinophilous vacuole. The DNA sequence ofM.catmrigalaewas clustered phylogenetically with otherMyxobolusspp. infecting the gills of cyprinids available in GenBank, which showed 77-87 % homogeneity. On the phylogenetic tree,M.catmrigalae(KC933944) was clustered withM.pavlovskii(HM991164) infecting the gill lamellae of silver carp,Hypophthalmichthysmolitrix. The species most closely related toM.catmrigalaein GenBank wasM.pavlovskii(AF507973) infecting the gill lamellae of big head carp,Aristichthysnobiliswith 87 % homogeneity. This is the first report on molecular characterization of gill lamellae infectingM. catmrigalae.
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5 - Budherhat Road, Chakgaria, Kolkata, West Bengal 700 094 India.
Sayani
S
Banerjee
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5 - Budherhat Road, Chakgaria, Kolkata, West Bengal 700 094 India.
Avijit
A
Patra
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5 - Budherhat Road, Chakgaria, Kolkata, West Bengal 700 094 India.
Anjan
A
Mondal
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5 - Budherhat Road, Chakgaria, Kolkata, West Bengal 700 094 India.
Harresh
H
Adikesavalu
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5 - Budherhat Road, Chakgaria, Kolkata, West Bengal 700 094 India.
Kurva Raghu
KR
Ramudu
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5 - Budherhat Road, Chakgaria, Kolkata, West Bengal 700 094 India.
Gadadhar
G
Dash
Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37 - Kshudiram Bose Sarani, Belgachia, Kolkata, West Bengal 700037 India.
Siddhartha Narayan
SN
Joardar
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5 - Budherhat Road, Chakgaria, Kolkata, West Bengal 700 094 India.
Thangapalam Jawahar
TJ
Abraham
Aquaculture; Indian major carps; Molecular characterization; Myxoboluscatmrigalae; Myxosporea; Phylogenetic analysis