5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila.
5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body. Furthermore, analysis of the en-expression pattern in the EADP provided strong evidence that the EADP is derived from one of the en-expressing head segments.
Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
Yu Shan
YS
Huang
Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan.|Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Hui Yu
HY
Ku
Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
Yun Chi
YC
Tsai
Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
Chin Hao
CH
Chang
Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
Sih Hua
SH
Pao
Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan.|Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Y Henry
YH
Sun
Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.|Biophotonics and Molecular Imaging Research Center, National Yang-Ming University, Taipei, Taiwan.
Arthur
A
Chiou
D000818:Animals; D015415:Biomarkers; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence / Q000379:methods*
D015415:Biomarkers