Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement.
Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.
Department of Health Sciences, University of Jaén, Jaén, Spain.|Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada, Spain.
Elena
E
López-Ruiz
School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, Edinburgh, UK.
Seshasailam
S
Venkateswaran
Department of Health Sciences, University of Jaén, Jaén, Spain.|Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada, Spain.
Macarena
M
Perán
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada, Spain.|Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Granada, Spain.|Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, Spain.
Gema
G
Jiménez
School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, Edinburgh, UK.
Salvatore
S
Pernagallo
Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Granada, Spain.
Juan J
JJ
Díaz-Mochón
Department of Pulmonary Medicine, Hospital Clínic; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.|Biomedical Research Networking Center on Respiratory Diseases (CIBERES), Madrid, Spain.
Olga
O
Tura-Ceide
Department of Histology, Faculty of Medicine, Institute of Neuroscience, Biomedical Research Centre, University of Granada, Granada, Spain.
Francisco
F
Arrebola
Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, Spain.|Department of Structural Mechanics, University of Granada, Politécnico de Fuentenueva, Granada, Spain.
Juan
J
Melchor
Department of Structural Mechanics, University of Granada, Politécnico de Fuentenueva, Granada, Spain.
Juan
J
Soto
Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, Spain.|Department of Structural Mechanics, University of Granada, Politécnico de Fuentenueva, Granada, Spain.
Guillermo
G
Rus
Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Granada, Spain.
Pedro J
0000-0001-7968-5353
PJ
Real
Department of Hemotherapy and Hemostasis, Hospital Clinic, Centre de Diagnostic Biomedic (CDB), Institute of Biomedical Research August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
María
M
Diaz-Ricart
School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, Edinburgh, UK.
Antonio
A
Conde-González
School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, Edinburgh, UK. mark.bradley@ed.ac.uk.
Mark
M
Bradley
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada, Spain. jmarchal@ugr.es.|Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Granada, Spain. jmarchal@ugr.es.|Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, Spain. jmarchal@ugr.es.
Juan A
JA
Marchal
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001704:Biopolymers; D001792:Blood Platelets / Q000502:physiology; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000502:physiology* / Q000648:ultrastructure; D002341:Carotid Artery Thrombosis / Q000517:prevention & control*; D042783:Endothelial Cells / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology; D006801:Humans; D008768:Methylmethacrylates / Q000737:chemistry*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D001704:Biopolymers; D008768:Methylmethacrylates; C009931:poly(ethylmethacrylate)