Effect of Vernonia amygdalina Del. Leaf Ethanolic Extract on Intoxicated Male Wistar Rats Liver.
Vernonia amygdalinahas been shown to have antioxidant activity, and is also expected to have hepatoprotective activity. This study was conducted to study the effect ofV. amygdalinaethanol extracts on intoxicated rat livers. Fresh leaves were extracted in ethanol, and the hepatoprotective activity was tested on male Wistar rats induced with a combination of isoniazid (INH) and rifampicin. Parameters observed were the activity of the enzyme alanine transferase (ALT), serum albumin levels, liver index, and histopathological of the rat liver. The results showed that 50 and 100 mg/kg rat body weight ofV. amygdalinaethanol extracts could prevent liver intoxication, starting on day 14. Based on serum albumin concentrations and ALT activity, the high dose extract (100 mg/kg) was more potent as a hepatoprotective agent compared to the extract at a low dose (50 mg/kg). The group of rats treated with a high dose extract showed normal liver index compared to the positive control. Through histology examination, the liver of rats treated with a high dose extract (100 mg/kg) showed minimal liver cell structure damage, and showed similar patterns to the normal rat. Based on these results, it can be concluded thatV. amygdalinaethanol extracts can be used to protect the liver in a combination of INH and rifampicin as antituberculosis treatment.
Pharmacology Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132, Indonesia. maria@fa.itb.ac.id.|Biotechnology Biomedical Research Center, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132, Indonesia.. maria@fa.itb.ac.id.
Maria Immaculata
MI
Iwo
Pharmacology Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132, Indonesia. sergialouisa@yahoo.co.id.
Sergia Louisa
SL
Sjahlim
Pharmacology Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132, Indonesia. siti.farah@fa.itb.ac.id.
Siti Farah
SF
Rahmawati
INH; Rifampicin; Vernonia amygdalina; intoxicated rat liver