Basophil Degranulation Assay.
Basophil degranulation assay has gained importance over the last decade in both diagnosis of food allergy and evaluation of progression of immunotherapy. This assay involves the identification and quantification of the expression of CD63 molecule on basophil membrane. CD63 is a marker of multivesicular bodies that is exposed to cell membrane during the process of degranulation in which the contents of basophil granules are released. This chapter describes the methodology for performing this assay.
Division of Allergy and Immunology, Department of Pediatrics, The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, Icahn Building, Room 11-23B, Box 1089, New York, NY, 10029, USA. madhan.masilamani@mssm.edu.|Immunology Institute and The Mindich Child Health and Development Institute, Mount Sinai School of Medicine, New York, NY, 10029, USA. madhan.masilamani@mssm.edu.
Madhan
M
Masilamani
Division of Allergy and Immunology, Department of Pediatrics, The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, Icahn Building, Room 11-23B, Box 1089, New York, NY, 10029, USA.
Mohanapriya
M
Kamalakannan
Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Hugh A
HA
Sampson
D015549:Basophil Degranulation Test / Q000379:methods; D001491:Basophils / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D005512:Food Hypersensitivity / Q000378:metabolism; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D060149:Tetraspanin 30 / Q000378:metabolism
D015415:Biomarkers; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30
Basophils; CD63; Degranulation; Flow cytometry; In vitro test