Chemical composition and inhibitory effects of water extract of Henna leaves on reactive oxygen species, DNA scission and proliferation of cancer cells.
From the centuries,Lawsonia inermisL. (Henna) is utilized in traditional health care system as a medicinal and cosmetic agent. The present study was intended to assess antiradical, DNA protective and antiproliferative activity of water extract ofLawsonia inermisL. leaves (W-LI). Antioxidant activity was estimated using variousin vitroassays such as DPPH, ABTS, superoxide anion radical scavenging, FRAP, deoxyribose degradation and DNA protection assay. Growth inhibitory effects of W-LI were assessed using MTT assay against different cancer cell linesviz.HeLa, MCF-7, A549, C6 and COLO-205. From the results of antioxidant assays, it was found that W-LI quenched DPPH and ABTS cation radicals with IC50value of 352.77 µg/ml and 380.87 µg/ml respectively. It demonstrated hydroxyl radical scavenging potential of 59.75 % at highest test dose of 1000 µg/ml in deoxyribose degradation assay. The results of FRAP assay showed that W-LI also possesses significant reducing activity. Extract inhibited hydroxyl radical induced pBR322 plasmid DNA strand scission, thus conferring DNA protection. Growth inhibition of various cancer cell lines was achieved to the varying extent on treatment with W-LI. Further, it was observed that activity was quite promising against colon cancer COLO-205 cells (GI50121.03 µg/ml). HPLC profiling of W-LI revealed the presence of different polyphenolic compounds such as ellagic acid, catechin, quercetin, kaempferol etc. which might be contributing towards antioxidant and cytotoxic activity. The present study demonstrated that polyphenols rich W-LI extract from leaves ofL. inermispossesses ability to inhibit oxidative radicals and cancer cells proliferation.
Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar-143005, Punjab, India; Akal College of Basic Sciences (Botany), Eternal University, Baru Sahib, Sirmour-173101, Himachal Pradesh, India.
Manish
M
Kumar
Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar-143005, Punjab, India.
Madhu
M
Chandel
Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar-143005, Punjab, India.
Paramjeet
P
Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar-143005, Punjab, India.
Kritika
K
Pandit
Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar-143005, Punjab, India.
Varinder
V
Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar-143005, Punjab, India.
Sandeep
S
Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar-143005, Punjab, India.
Satwinderjeet
S
Kaur
HPLC; Lawsonia inermis L.; antioxidant activity; cytotoxic; polyphenols