Investigation of antiaromatase activity using hepatic microsomes of Nile tilapia (Oreochromis niloticus).
Microsomal aromatase enzymes of humans and rats have been used in antiaromatase assays, but enzyme activity is species-specific. The current study extracted hepatic microsomes of Nile tilapia (Oreochromis niloticus) to investigate and compare the antiaromatase activity of chrysin, quercetin, and quercitrin. This activity was evaluated using a dibenzylfluorescein (DBF) assay. Results revealed that the age and body weight of Nile tilapia affected the yield of extracted microsomes. Extraction of hepatic microsomes of Nile tilapia was most effective when using a reaction medium with a pH of 8.0. A DBF assay using Nile tilapia microsomes revealed significant differences in levels of antiaromatase activity for chrysin, quercetin, and quercitrin. Chrysin was the most potent aromatase inhibitor, with an IC50 of 0.25 mg/mL. In addition, chrysin is an aromatase inhibitor that also inhibits the proliferation of cancer cells. Hepatic microsomes of Nile tilapia can be used to investigate and compare the antiaromatase activity of different compounds.
Nanoscience and Nanotechnology Program, the Graduate School, Chiang Mai University.
Tanongsak
T
Sassa-Deepaeng
Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University.
Wasana
W
Chaisri
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University.
Surachai
S
Pikulkaew
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University.
Siriporn
S
Okonogi
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D047072:Aromatase Inhibitors / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D023681:Cichlids; D005419:Flavonoids / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D008855:Microscopy, Electron, Scanning; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000975:Antioxidants; D047072:Aromatase Inhibitors; D005419:Flavonoids; C012526:quercitrin; C043561:chrysin; D011794:Quercetin
Aromatase inhibitor; Nile tilapia; chrysin; microsome; pH effect