Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation.
Cyclin proteins are the key regulatory and activity partner of cyclin-dependent kinases (CDKs), which play pivotal regulatory roles in cell cycle progression. In the present study, we identified a Cyclin L1 and 2 CDK11 2 CDK11 splice variants, CDK11A and CDK11B, from silkworm, Bombyx mori. We determined that both Cyclin L1 and CDK11A/B are nuclear proteins, and further investigations were conducted to elucidate their spatiofunctional features. Cyclin L1 forms a complex with CDK11A/B and were co-localized to the nucleus. Moreover, the dimerization of CDK11A and CDK11B and the effects of Cyclin L1 and CDK11A/B on cell cycle regulation were also investigated. Using overexpression or RNA interference experiments, we demonstrated that the abnormal expression of Cyclin L1 and CDK11A/B leads to cell cycle arrest and cell proliferation suppression. Together, these findings indicate that CDK11A/B interacts with Cyclin L1 to regulate the cell cycle.
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Tai-Hang
TH
Liu
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Yun-Fei
YF
Wu
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.|b College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.
Xiao-Long
XL
Dong
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Cai-Xia
CX
Pan
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Guo-Yu
GY
Du
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Ji-Gui
JG
Yang
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Wei
W
Wang
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Xi-Yan
XY
Bao
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.
Peng
P
Chen
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.|c Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry , Southwest University , Chongqing , China.
Min-Hui
MH
Pan
a State Key Laboratory of Silkworm Genome Biology , Southwest University , Chongqing , China.|c Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry , Southwest University , Chongqing , China.
Cheng
C
Lu
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation; D003001:Cloning, Molecular; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D019913:Nuclear Localization Signals; D010802:Phylogeny; D055503:Protein Multimerization
D016213:Cyclins; D019476:Insect Proteins; D019913:Nuclear Localization Signals; D018844:Cyclin-Dependent Kinases
Bombyx mori; CDK11; NLS; cell cycle; cyclin L1