Ex-Vivo Tissues Engineering Modeling for Reconstructive Surgery Using Human Adult Adipose Stem Cells and Polymeric Nanostructured Matrix.
The major challenge for stem cell translation regenerative medicine is the regeneration of damaged tissues by creating biological substitutes capable of recapitulating the missing function in the recipient host. Therefore, the current paradigm of tissue engineering strategies is the combination of a selected stem cell type, based on their capability to differentiate toward committed cell lineages, and a biomaterial, that, due to own characteristics (e.g., chemical, electric, mechanical property, nano-topography, and nanostructured molecular components), could serve as active scaffold to generate a bio-hybrid tissue/organ. Thus, effort has been made on the generation ofin vitrotissue engineering modeling. Here, we present anin vitromodel where human adipose stem cells isolated from lipoaspirate adipose tissue and breast adipose tissue, cultured on polymeric INTEGRA®Meshed Bilayer Wound Matrix (selected based on conventional clinical applications) are evaluated for their potential application for reconstructive surgery toward bone and adipose tissue. We demonstrated that human adipose stem cells isolated from lipoaspirate and breast tissue have similar stemness properties and are suitable for tissue engineering applications. Finally, the overall results highlighted lipoaspirate adipose tissue as a good source for the generation of adult adipose stem cells.
Department of Chemistry, Biology and Biotechnologies, Biochemistry and Molecular Biology Unit, University of Perugia, Via del Giochetto, Perugia 06122, Italy. effemorena@gmail.com.
Francesco
F
Morena
Department of Chemistry, Biology and Biotechnologies, Biochemistry and Molecular Biology Unit, University of Perugia, Via del Giochetto, Perugia 06122, Italy. chiara.argentati89@gmail.com.
Chiara
C
Argentati
Department of Chemistry, Biology and Biotechnologies, Biochemistry and Molecular Biology Unit, University of Perugia, Via del Giochetto, Perugia 06122, Italy. eleonoracalzoni@gmail.com.
Eleonora
E
Calzoni
Unità Operativa Chirurgia Plastica e Ricostruttiva, ASL 1 Umbria, Città di Castello 06012, Italy. marinocordellini@yahoo.it.
Marino
M
Cordellini
Department of Chemistry, Biology and Biotechnologies, Biochemistry and Molecular Biology Unit, University of Perugia, Via del Giochetto, Perugia 06122, Italy. carla.emiliani@unipg.it.
Carla
C
Emiliani
Department of Chemistry, Biology and Biotechnologies, Biochemistry and Molecular Biology Unit, University of Perugia, Via del Giochetto, Perugia 06122, Italy. francesco.dangelo@angelantoni.it.
Francesco
F
D'Angelo
Department of Chemistry, Biology and Biotechnologies, Biochemistry and Molecular Biology Unit, University of Perugia, Via del Giochetto, Perugia 06122, Italy. sabata.martino@unipg.it.
Sabata
S
Martino
INTEGRA®; adult mesenchymal stem cells isolation and culture; regenerative medicine; stem cells and biomaterial interaction