A putative 12-oxophytodienoate reductase gene CsOPR3 from Camellia sinensis, is involved in wound and herbivore infestation responses.
12-Oxophytodienoate reductase (OPR) is a key enzyme in the biosynthesis of jasmonic acid (JA), which plays an important role in plant defense responses. Although multiple isoforms of OPRs have been identified in various annual herbaceous plants, genes encoding these enzymes in perennial woody plants have yet to be fully investigated. In the tea plant, Camellia sinensis (L.), no OPR genes have been isolated, and their possible roles in tea plant development and defense mechanism remain unknown. In this study, a putative OPR gene, designated as CsOPR3, was isolated from tea plants for the first time through the rapid amplification of cDNA ends. The open reading frame of CsOPR3 is 1197bp in length, and encodes a protein of 398 amino acids. Real-time qPCR analysis revealed that CsOPR3 was expressed in different organs. In particular, CsOPR3 was highly expressed in flowers, leaves and stems but was weakly expressed in roots and seeds. CsOPR3 expression could be rapidly induced by mechanical wounding, and increased JA levels were correlated with the wound-induced CsOPR3 expression. The infestation of the tea geometrid (TG) Ectropis obliqua Prout, regurgitant derived from TG and exogenous JA application could enhance the CsOPR3 expression. Our study is the first to report that CsOPR3 plays an important role in JA biosynthesis and tea plant defense against herbivorous insects.
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China.
Zhaojun
Z
Xin
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China.
Jin
J
Zhang
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China.
Lingang
L
Ge
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China.
Shu
S
Lei
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China.
Juanjuan
J
Han
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China.
Xin
X
Zhang
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China.
Xiwang
X
Li
Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou 310008, China. Electronic address: xlsun1974@163.com.
Xiaoling
X
Sun
D000818:Animals; D028241:Camellia sinensis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D060434:Herbivory; D009036:Moths; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics* / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase
12-Oxophytodienoate reductase; Herbivore infestation; Jasmonic acid; Tea; Wounding