Antimicrobial Properties of Biofunctionalized Silver Nanoparticles on Clinical Isolates of Streptococcus mutans and Its Serotypes.
(1) Background:Streptococcus mutans(S. mutans) is the principal pathogen involved in the formation of dental caries. Other systemic diseases have also been associated with specificS. mutansserotypes (c,e,f, andk). Silver nanoparticles (SNP) have been demonstrated to have good antibacterial effects againstS. mutans; therefore, limited studies have evaluated the antimicrobial activity of biofunctionalized SNP onS. mutansserotypes. The purpose of this work was to prepare and characterize coated SNP using two different organic components and to evaluate the antimicrobial activity of SNP in clinical isolates ofS. mutansstrains and serotypes; (2) Methods: SNP with bovine serum albumin (BSA) or chitosan (CS) coatings were prepared and the physical, chemical and microbiological properties of SNP were evaluated; (3) Results: Both types of coated SNP showed antimicrobial activity againstS. mutansbacteria and serotypes. Better inhibition was associated with smaller particles and BSA coatings; however, no significant differences were found between the different serotypes, indicating a similar sensitivity to the coated SNP; (4) Conclusion: This study concludes that BSA and CS coated SNP had good antimicrobial activity againstS. mutansstrains and the four serotypes, and this study suggest the widespread use of SNP as an antimicrobial agent for the inhibition ofS. mutansbacteria.
Graduate Program in Orthodontics, Faculty of Dentistry, Mexicali Campus, Autonomous University of Baja California, Alvaro Obregon and Julian Carrillo Avenue, Nueva, 21100 Mexicali, Baja California, México. angelorto@outlook.es.
Ángel Manuel
ÁM
Martínez-Robles
Master Program in Advanced Dentistry, Faculty of Dentistry, Autonomous University of San Luis Potosi, Manuel Nava Avenue, Universitary Campus, 78290 San Luis Potosí, S. L. P., México. juanpablo.loyola8@gmail.com.
Juan Pablo
JP
Loyola-Rodríguez
Doctoral Program in Dental Sciences, Faculty of Dentistry, Autonomous University of San Luis Potosi, Manuel Nava Avenue, Universitary Campus 78290 San Luis Potosí, S. L. P., México. nveroza@hotmail.com.
Norma Verónica
NV
Zavala-Alonso
Master Program in Advanced Dentistry, Faculty of Dentistry, Autonomous University of San Luis Potosi, Manuel Nava Avenue, Universitary Campus, 78290 San Luis Potosí, S. L. P., México. ritae_martinez@hotmail.com.
Rita Elizabeth
RE
Martinez-Martinez
Faculty of Science, Autonomous University of San Luis Potosi, Salvador Nava Avenue, 78290 San Luis Potosí, S. L. P., México. facundo@fciencias.uaslp.mx.
Facundo
F
Ruiz
Faculty of Chemistry, Juarez University of Durango State, Chihuahua Avenue, 34120 Durango, Dgo., México. lcrh75@hotmail.com.
René Homero
RH
Lara-Castro
Department of Dentistry, Biomedical Science Institute, Autonomous University of Juarez City, Envolvente del PRONAF and Estocolmo Avenues, 32310 Juárez, Chihuahua, México. adonohue@uacj.mx.
Alejandro
A
Donohué-Cornejo
Biomedical Science Institute, Autonomous University of Juarez City, Envolvente del PRONAF and Estocolmo Avenues, 32310 Juárez, Chihuahua, México. yobannyr@yahoo.com.mx.
Simón Yobanny
SY
Reyes-López
Department of Dentistry, Biomedical Science Institute, Autonomous University of Juarez City, Envolvente del PRONAF and Estocolmo Avenues, 32310 Juárez, Chihuahua, México. leohamet@hotmail.com.
León Francisco
LF
Espinosa-Cristóbal
Streptococcus mutans; antimicrobial effect; bovine serum albumin; chitosan; silver nanoparticles