Microsequential injection lab-on-valve system for the spectrophotometric bi-parametric determination of iron and copper in natural waters.
The determination of iron and copper exploiting a microsequential injection lab-on-valve system with online spectrophotometric detection is described. A new, environmental friendly 3-hydroxy-4-pyridinone chelator, functionalized with a polyethylene glycol chain (MRB12) to improve water solubility, was used for iron determination. For copper determination, 1-(2-pyridylazo)-2-naphthol (PAN) was used. Different parameters affecting the formation of the complexes were studied, namely sample volume, reagent concentration, and buffer composition and concentration. The optimized conditions, 150µL of sample volume and 250mgL-1of MRB12 for iron determination, and 200µL of sample and 120mgL-1of PAN for copper determination, enabled an LOD of 15 and 18µgL-1for iron and copper, respectively. The robustness of the developed procedure was assessed by the calculation of the relative standard deviation (RSD), 5% for iron determination and 2% for copper determination. The accuracy of the method was assessed comparing the results with two certified samples (RD<7.5%) and calculating recovery percentages with five river water samples (average<107%).
Group of Analytical Chemistry, Automation and Environment, Department of Chemistry, University of the Balearic Islands, E-07122 Palma de Mallorca, Spain.
Alba
A
González
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, R. Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal. Electronic address: rmesquita@porto.ucp.pt.
Raquel B R
RBR
Mesquita
Sciware Systems, S.L. Spin-off of the University of the Balearic Islands, E-07193 Bunyola, Spain.
Jessica
J
Avivar
REQUIMTE-UCIBIO, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, 4169-007 Porto, Portugal.
Tânia
T
Moniz
REQUIMTE-UCIBIO, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal.
Maria
M
Rangel
Group of Analytical Chemistry, Automation and Environment, Department of Chemistry, University of the Balearic Islands, E-07122 Palma de Mallorca, Spain; Sciware Systems, S.L. Spin-off of the University of the Balearic Islands, E-07193 Bunyola, Spain.
Víctor
V
Cerdà
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, R. Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
António O S S
AOSS
Rangel
D003300:Copper / Q000032:analysis*; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D005618:Fresh Water / Q000032:analysis*; D007501:Iron / Q000032:analysis*; D009284:Naphthols / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011728:Pyridones / Q000737:chemistry; D045483:Rivers / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*
D009284:Naphthols; D011728:Pyridones; C041728:1-(2-pyridylazo)-2-naphthol; C020366:3-hydroxy-4-pyridone; D011092:Polyethylene Glycols; D003300:Copper; D007501:Iron
1-(2-pyridylazo)-2-naphthol; Bi-parametric determination; Iron and copper; PEGylated 3-hydroxy-4-pyridinone; Water analysis