Ovicidal effect of chitinase and protease enzymes produced by soil fungi on the camel tickHyalomma dromedariieggs (Acari:Ixodidae).
The aim of this study was to evaluate the effect of chitinase and protease enzymes produced by environmentally safe soil Fungi;Aspergillussp. NRC 4/5H;Mucorsp. NRC 5;Trichodermasp. NRC 4/56;Aspergillus sojae;Mucor ranosisinus;Aspergillus oryzaeon embryo development of the camel tickHyalomma dromedariieggs. The experiment was carried out on the 7 day aged eggs. Concentrations of the chitinase and protease crude enzymes [1:1, 1:2, 1:3, 1:4 and 1:5 (v/v)] were prepared from six stock solutions whose concentrations were 5 units/ml each. The prepared chitinase and protease enzymes produced by soil fungi were tested in vitro study for eradication of ticks. Ovicidal effect of the chitin concentrations of extracellular lytic enzymes (chitinase) produced byAspergillussp NRC 4/5H,Mucorsp. NRC 5,Trichodermasp. NRC 4/56 were tested onH. dromedariieggs. The results showed that the ovicidal effect increased with increasing the chitin concentration in case ofMucorsp. NRC 5. The maximum inhibitory effect which ranged from 95.3 to 100 % was at concentrations ranging from 1:3 to 1:5 ml/ml, respectively. The results of protease enzymes produced byA. sojae,M. ranosisinusandA. oryzaerevealed that, it is highly effective in all concentrations onH.dromedariieggs. It is indicated that the chitinase and protease enzymes produced by fungal species could be used for biological control of the camel tick infestation to avoid the use of carcinogenic chemical.
Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, Post Box: 12622, El Bohouth Street, Dokki, Cairo, Egypt.
Salwa M
SM
Habeeb
Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, Post Box: 12622, El Bohouth Street, Dokki, Cairo, Egypt.
Heba M
HM
Ashry
Microbial Chemistry Department, National Research Centre, P.O. Box 12622, El-Bohouth Street, Dokki, Cairo, Egypt.
Moataza M
MM
Saad
Chitinase; Eggs; Fungi; Hyalomma dromedarii; Protease; Tick