Characterization of stromal vascular fraction and adipose stem cells from subcutaneous, preperitoneal and visceral morbidly obese human adipose tissue depots.
OBJECTIVE
The pathological condition of obesity is accompanied by a dysfunctional adipose tissue. We postulate that subcutaneous, preperitoneal and visceral obese abdominal white adipose tissue depots could have stromal vascular fractions (SVF) with distinct composition and adipose stem cells (ASC) that would differentially account for the pathogenesis of obesity.


METHODS
In order to evaluate the distribution of SVF subpopulations, samples of subcutaneous, preperitoneal and visceral adipose tissues from morbidly obese women (n = 12, BMI: 46.2±5.1 kg/m2) were collected during bariatric surgery, enzymatically digested and analyzed by flow cytometry (n = 12). ASC from all depots were evaluated for morphology, surface expression, ability to accumulate lipid after induction and cytokine secretion (n = 3).


RESULTS
A high content of preadipocytes was found in the SVF of subcutaneous depot (p = 0.0178). ASC from the three depots had similar fibroblastoid morphology with a homogeneous expression of CD34, CD146, CD105, CD73 and CD90. ASC from the visceral depot secreted the highest levels of IL-6, MCP-1 and G-CSF (p = 0.0278). Interestingly, preperitoneal ASC under lipid accumulation stimulus showed the lowest levels of all the secreted cytokines, except for adiponectin that was enhanced (p = 0.0278).


CONCLUSIONS
ASC from preperitoneal adipose tissue revealed the less pro-inflammatory properties, although it is an internal adipose depot. Conversely, ASC from visceral adipose tissue are the most pro-inflammatory. Therefore, ASC from subcutaneous, visceral and preperitoneal adipose depots could differentially contribute to the chronic inflammatory scenario of obesity.
Post-graduation Program of Medical Clinics, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil.|Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology - INMETRO, Duque de Caxias, Rio de Janeiro, Brazil.
Karina Ribeiro
KR
Silva
Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology - INMETRO, Duque de Caxias, Rio de Janeiro, Brazil.
Isis
I
Côrtes
Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation - FIOCRUZ, Rio de Janeiro, Brazil.
Sally
S
Liechocki
Nutrology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil.
João Regis Ivar
JR
Carneiro
Surgery Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Antônio Augusto Peixoto
AA
Souza
Post-graduation Program of Medical Clinics, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil.|Center of Regenerative Medicine, Petrópolis Faculty of Medicine - FASE, Petrópolis, Rio de Janeiro, Brazil.
Radovan
R
Borojevic
Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation - FIOCRUZ, Rio de Janeiro, Brazil.
Clarissa Menezes
CM
Maya-Monteiro
Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology - INMETRO, Duque de Caxias, Rio de Janeiro, Brazil.|Nucleus of Multidisciplinary Research in Xerem - Biology (Numpex-Bio), Federal University of Rio de Janeiro - Xerém, Duque de Caxias, Rio de Janeiro, Brazil.
Leandra Santos
http://orcid.org/0000-0001-9998-8044
LS
Baptista
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000473:pathology; D050152:Intra-Abdominal Fat / Q000473:pathology*; D009767:Obesity, Morbid / Q000473:pathology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*
D016207:Cytokines