Degradation and selective ligninolysis of wheat straw and banana stem for an efficient bioethanol production using fungal and chemical pretreatment.
Lignocelluloses from agricultural, industrial, and forest residues constitute a majority of the total biomass present in the world. Environmental concerns of disposal, costly pretreatment options prior to disposal, and increased need to save valuable resources have led to the development of value-added alternate technologies such as bioethanol production from lignocellulosic wastes. In the present study, biologically pretreated (with the fungus, Pleurotus ostreatus HP-1) and chemically pretreated (with mild acid or dilute alkali) wheat straw (WS) and banana stem (BS) were subsequently subjected to enzymatic saccharification (with mixture of 6.0 U/g of filter paper cellulase and 17 U/g of β-glucosidase) and were evaluated for bioethanol production using Saccharomyces cerevisiae NCIM 3570. Biological and chemical pretreatments removed up to 4.0-49.2 % lignin from the WS and BS which was comparatively higher than that for cellulose (0.3-12.4 %) and for hemicellulose (0.7-21.8 %) removal with an average 5.6-49.5 % dry matter loss. Enzymatic hydrolysis yielded 64-306.6 mg/g (1.5-15 g/L) reducing sugars from which 0.15-0.54 g/g ethanol was produced from Saccharomyces cerevisiae NCIM 3570.
Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, 388120, Gujarat, India.
Shilpi
S
Thakur
Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi, South Campus, New Delhi, 110021, India.
Bhuvnesh
B
Shrivastava
Ashok and Rita Patel Institute of Integrated Study and Research in biotechnology and Allied Sciences, New Vallabh Vidyanagar, 388121, Gujarat, India.
Snehal
S
Ingale
Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi, South Campus, New Delhi, 110021, India.
Ramesh C
RC
Kuhad
Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, 388120, Gujarat, India. akshaya_gupte@hotmail.com.
Akshaya
A
Gupte
Bioethanol; Lignocelluloses; Pleurotus ostreatus HP-1; Pretreatment; Saccharification