High-performance thin layer chromatography based assay and stress study of a rare steroidal alkaloid solanopubamine in six species ofSolanumgrown in Saudi Arabia.
The present study describes a method developed for quantification and stability study of a rare steroidal alkaloid solanopubamine (SPN) in aerial parts of six different species of genusSolanumextracted with two different solvents. TheSolanumspecies selected for investigation includeS. schimperianum(SS),S. villosum(SV),S. coagulans(SC),S. glabratum(SG),S. incanum(SI) andS. nigrum(SN). The estimation of SPN was done by a validated high-performance thin layer chromatography method. The developed chromatographic system was found to give a sharp spot for solanopubamine atRf= 0.39 ± 0.01. The steroidal alkaloid SPN was observed to be present only in extracts of aerial parts ofS. schimperianum. The sensitivity of developed method produced 40 ng and 115 ng band-1, respectively as LOD and LOQ values. The percentage yield of SPN in aerial parts ofS. schimperianumextracted by ethanol (95%) only and a mixture of ethanol and ammonium hydroxide (6:4) was found to be 1.03 w/w and 2.09 w/w, respectively. Stability studies of SPN exhibited the maximum (100%) degradation in an alkaline environment and H2O2treated samples and 61.4% in acidic conditions. The SPN was found to be significantly stable against UV exposure, photo-oxidation and at room temperature while 13.83% and 57.88% destruction has been observed when exposed to dry heat at 40 °C and 60 °C, respectively.
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Nasir A
NA
Siddiqui
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Mohammad K
MK
Parvez
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Adnan J
AJ
Al-Rehaily
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Mohammed S
MS
Al Dosari
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Perwez
P
Alam
Center of Excellence in Biotechnology Research, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia; Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Faiyaz
F
Shakeel
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Hattan A
HA
Al Harbi
HPTLC; Quantification; Solanopubamine; Solanum species; Stability study; Steroidal alkaloids