Antidiuretic and antinatriuretic response to high salt load in normotensive Wistar-Kyoto rats: Role of alpha-1A-adrenoreceptors.
Altered renal adrenergic responses have been recognized as pathophysiological responses to high salt intake. This study aims to investigate the influence of 6 weeks of high salt diet on α1A-adrenoceptor regulation of renal tubular antinatriuretic and antidiuretic response in normal Wistar Kyoto rats. To achieve the above objective, antinatriuretic and antidiuretic response to phenylephrine was measured in the absence and presence of 5-methylurapidil (5-MeU) using the inulin clearance method. Systemic mean arterial blood pressure and renal haemodynamics were also measured simultaneously. Six weeks of high salt intake in Wistar-Kyoto (WKY) rats did not bring any significant increase in mean arterial blood pressure. WKY rat on high salt diet (WKYHNa) showed an exaggerated increase in absolute and fractional sodium excretion. There was a significant involvement of α1A-adrenoceptor in carrying out renal tubular antinatriuretic and antidiuretic response in Wistar Kyoto rats on normal sodium diet (WKYNNa). However, α1A-adrenoceptor played a minimal role in handling the tubular reabsorptive response in WKY rats on high salt diet.
College of Applied Medical Science, Prince Sattam Bin Abdul-Aziz University, Wadi Ad Dawaser, Saudi Arabia.|School of Pharmaceutical Sciences, University Sains Malaysia, Penang, Malaysia.
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http://orcid.org/0000-0003-4547-6427
RN
Kazi
School of Pharmaceutical Sciences, University Sains Malaysia, Penang, Malaysia.
M A
MA
Sattar
Department of Physiology, Aras Windle, University College Cork, Cork, Ireland.
E J
EJ
Johns
D000818:Animals; D050034:Antidiuretic Agents / Q000494:pharmacology*; D062186:Arterial Pressure / Q000187:drug effects; D007328:Insulin / Q000378:metabolism; D008297:Male; D045283:Natriuretic Agents / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY; D018340:Receptors, Adrenergic, alpha-1 / Q000187:drug effects*; D012079:Renal Circulation; D017673:Sodium Chloride, Dietary / Q000494:pharmacology*
D050034:Antidiuretic Agents; D007328:Insulin; D045283:Natriuretic Agents; D010879:Piperazines; D018340:Receptors, Adrenergic, alpha-1; D017673:Sodium Chloride, Dietary; C057446:5-methylurapidil; D010656:Phenylephrine
WKY rats; antidiuresis; antinatriuresis; high salt; α1A-adrenoceptor