Noncoding miRNAs bridge virus infection and host autophagy in shrimpin vivo.
Autophagy is known to be involved in viral infection. However, the relationship between autophagy and viral infection has not been characterized. MicroRNAs (miRNAs), important regulators of gene transcription, play central roles in many biologic processes, including autophagy and viral infection. It was therefore of interest to investigate the miRNAs regulating viral infection and host autophagy. In this study the viral infection (white spot syndrome virus, WSSV) correlated positively with the host autophagy in shrimp. Two host miRNAs (miR-71 and -13b) had significant effects on viral infection and host autophagy. MiR-71 regulated the viral infection and host autophagy by targeting the host calcification-associated peptide-1 (cap-1) gene. MiR-13b targeted the host knickkopf gene to simultaneously regulate viral infection and host autophagy. When the expressions of miR-71 and -13b were silenced in shrimp, the autophagy could not be induced by WSSV infection and the autophagy induction did not influence the virus replication, indicating that miR-71 and -13b were necessary for the viral infection and host autophagy. In this context, the findings of this study revealed a novel mechanism of miRNAs bridging the viral infection and host autophagyin vivo-He, Y., Sun, Y., Zhang, X. Noncoding miRNAs bridge virus infection and host autophagy in shrimpin vivo.
College of Life Sciences and Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, China.
Yaodong
Y
He
College of Life Sciences and Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, China.
Yuechao
Y
Sun
College of Life Sciences and Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, China zxb0812@zju.edu.cn.
Xiaobo
X
Zhang
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D033561:Penaeidae / Q000821:virology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
D035683:MicroRNAs
WWSV; interaction; rapamycin; target gene