Biogenic synthesis of Marsilea quadrifolia gold nanoparticles: a study of improved glucose utilization efficiency on 3T3-L1 adipocytes.
This study aims mainly to provide an insight and understanding of the effect of glucose utilization efficiency of biogenic gold nanoparticles (GNPs) synthesized through the mediation of Marsilea quadrifolia (M. quadrifolia) methanol extract on 3T3-L1 adipocytes. The biosynthesized GNPs were characterized by UV visible spectrophotometry and FTIR. Simultaneously, the nature, stability, and morphological characteristics were analyzed by XRD, TG-DTA, SEM-EDS, HRTEM, and SAED. The results of characterization studies were used to assess the properties of GNPs. The in vitro cytotoxicity screening indicates that 100 μM of biogenic GNPs were displayed 71.23 ± 1.56% of cellular viability in 3T3-L1 adipocyte cells. Subsequently, increased glucose utilization of biosynthesized GNPs based on a dose-dependent manner on 3T3-L1 has also been demonstrated. The effect of GNPs (30 μg) on glucose uptake was higher than that of insulin and metformin. Moreover, the observed results clearly highlight that the biogenic GNPs have higher efficiency of glucose utilization and cellular viability in 3T3-L1 adipocytes with lower toxicity.
Department of Chemical Engineering, Jadavpur University, Kolkata, 700032, India.
Anindita
A
Chowdhury
Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam University, Krishnankoil, 626126, India.
Selvaraj
S
Kunjiappan
Department of Chemical Engineering, Jadavpur University, Kolkata, 700032, India. cbhattacharyya@chemical.jdvu.ac.in.
Chiranjib
C
Bhattacharjee
Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam University, Krishnankoil, 626126, India.
Balasubramanian
B
Somasundaram
Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam University, Krishnankoil, 626126, India.
Theivendren
T
Panneerselvam
D041721:3T3-L1 Cells / Q000187:drug effects; D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D043964:Marsileaceae / Q000737:chemistry; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D010936:Plant Extracts; D006046:Gold; D005947:Glucose
Adipocytes; Cytotoxicity; Glucose; Gold nanoparticles; Marsilea quadrifolia