Effects of extracts and compounds from Tricholoma populinum Lange on degranulation and IL-2/IL-8 secretion of immune cells.
Tricholoma populinum Lange is an edible basidiomycete from the family Tricholomataceae. Extracts, fractions, and different metabolites isolated from the fruiting bodies of this mushroom were tested for degranulation-inhibiting activities on RBL-2H3 cells (rat basophils). Dichloromethane extracts decreased degranulation significantly, as did a fraction after column chromatography. In addition, the extract decreased the IL-2 release from Jurkat T cells and the release of IL-8 from HMC-1 human mast cells. The results show the significant effects of extracts of T. populinum on cells of the innate (basophils and mast cells) and adaptive (T cells) immune system and indicate the influence of the mushroom on different immunological processes. As one fraction showed activity, it seems to be possible that it includes an active principle. The compounds responsible for this effect, however, could not be identified as the contents oleic acid (1), ergosterol peroxide (2), and 9,11-dehydroergosterol peroxide (3) showed no effects. Nevertheless, the mushroom could be used for supporting allergy treatment in future studies.
Institute of Pharmacy, Pharmaceutical Biology, Ernst-Moritz-Arndt University Greifswald, Felix-Hausdorff-Str. 1, 17487 Greifswald, Germany.
Simon
S
Merdivan
Institute of Pharmacy, Freie Universität Berlin, Berlin, Germany.
Kristina
K
Jenett-Siems
AnalytiCon Discovery GmbH, Potsdam, Germany.
Karsten
K
Siems
Interfaculty Institute of Microbiology and Infection Medicine, Eberhard Karls University, Tübingen, Germany.|German Centre for Infection Research (DZIF), Partner Site, Tübingen, Germany.
Timo H J
THJ
Niedermeyer
Institute of Pharmacy, Ernst-Moritz-Arndt University Greifswald, Greifswald, Germany.
Nadin
N
Schultze
Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Carmen
C
Steinborn
Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Carsten
C
Gründemann
Institute of Pharmacy, Ernst-Moritz-Arndt University Greifswald, Greifswald, Germany.
Ulrike
U
Lindequist
D000818:Animals; D001491:Basophils / Q000187:drug effects* / Q000502:physiology; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel / Q000379:methods; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D019169:Jurkat Cells; D009682:Magnetic Resonance Spectroscopy; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008752:Methylene Chloride / Q000737:chemistry; D051381:Rats; D058428:Silica Gel / Q000737:chemistry; D055439:Tricholoma / Q000737:chemistry*
D001688:Biological Products; D007376:Interleukin-2; D016209:Interleukin-8; D008752:Methylene Chloride; D058428:Silica Gel
HMC-1 cells; Interleukin; Jurkat T cells; RBL-2H3; Sterols; Tricholoma populinum