Differential expression patterns of two delta-9-acyl-CoA desaturases inThitarodes pui(Lepidoptera: Hepialidae) during different seasons and cold exposure.
Thitarodes puilarvae have a limited distribution in the Tibetan Plateau and are the host of a parasitic fungus,Ophiocordyceps sinensis. Low temperature is a main environmental stress. However, understanding ofT. puicold adaptation mechanisms is insufficient. Delta-9-acyl-CoA desaturase (D9D) is closely correlated with cold adaptation for many organisms. To further understand the cold adaptation processes inT. puilarvae, twoD9Ds,TpdesatAandTpdesatBwere sequenced, and expression patterns were investigated during different seasons and cold exposure (under 0°C) in the laboratory. The full lengths of two cDNAs are 1,290 bp and 1,603 bp, and the ORFs encode a polypeptide of 348 and 359 amino acids, respectively. Four transmembrane domains, three conserved histidine residues and five hydrophobic regions exist in these two sequences. The expression level ofTpdesatAis up-regulated in the long-term cold exposure and negatively correlated with temperature in seasonal patterns.TpdesatBresponds to cold temperature in short-term cold exposure and positively corresponds temporarily in seasonal expression. TwoD9Ds may have different substrate specificities,TpdesatAtends to use C16:0 and C18:0 as substrate whileTpdesatBprefers C18:0. In conclusion,TpdesatAmay play a very important role inT. puicold tolerance andTpdesatBregulates function in short-term cold exposure and content change of fatty acids in the body.
School of life sciences Nanchang University Nanchang China.
Qiang
Q
Min
School of life sciences Nanchang University Nanchang China.
Shiyu
S
Cheng
School of life sciences Nanchang University Nanchang China.
Jianfei
J
Xi
School of life sciences Nanchang University Nanchang China.
Tianrong
T
Xin
School of life sciences Nanchang University Nanchang China.
Bin
B
Xia
School of life sciences Nanchang University Nanchang China.
Zhiwen
0000-0002-4362-9699
Z
Zou
Thitarodes pui; acclimation; delta‐9‐acyl‐CoA desaturases; experimental evolution; thermal adaptation