Oil-based adjuvants delivered intradermally induce high primary IgG2 immune response in swine.
The effects of intradermal application of antigen with or without different adjuvants and activation of immune response are presented in this study. Six groups of six piglets each were immunized with keyhole limpet haemocyanin (KLH) antigen in combination with aluminium hydroxide or oil-based adjuvants (complete and incomplete Freund's adjuvants, Montanide ISA 206 and Emulsigen). IgG1 and IgG2 levels in sera were measured by KLH-specific ELISA. Interestingly, oil-based adjuvants induced high primary IgG2 response, suggesting the Th1 lymphocyte polarization. Also, considering the similarities between human and porcine organism, we suggest that intradermal application could be considered as an effective vaccine delivery route in both veterinary and human medicine.
Department of Immunology, Veterinary Research Institute, Brno, Czech Republic; Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address: oreskovic@vri.cz.
Zrinka
Z
Oreskovic
Department of Immunology, Veterinary Research Institute, Brno, Czech Republic. Electronic address: kudlackova@vri.cz.
Hana
H
Kudlackova
Department of Immunology, Veterinary Research Institute, Brno, Czech Republic. Electronic address: krejci@vri.cz.
Josef
J
Krejci
Department of Immunology, Veterinary Research Institute, Brno, Czech Republic. Electronic address: nechvatalova@vri.cz.
Katerina
K
Nechvatalova
Department of Immunology, Veterinary Research Institute, Brno, Czech Republic. Electronic address: faldyna@vri.cz.
Martin
M
Faldyna
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006433:Hemocyanins / Q000494:pharmacology*; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood*; D008055:Lipids / Q000494:pharmacology*; D013552:Swine; D014611:Vaccination / Q000662:veterinary
D000276:Adjuvants, Immunologic; D000941:Antigens; D007074:Immunoglobulin G; D008055:Lipids; C114843:incomplete Freund's adjuvant; D005620:Freund's Adjuvant; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
Adjuvant; Immunity; Intradermal; Th1/Th2; Vaccine