Is CuIICoordinated to Patellamides inside Prochloron Cells?
Dinuclear CuII-patellamide complexes (patellamides are naturally occurring cyclic pseudo-octapeptides) are known to be efficient catalysts for hydrolysis reactions of biological importance, for example, those of phosphatase, carbonic anhydrase, and glycosidase. However, the biological role of patellamides is still unknown. Patellamides were originally extracted from the sea squirt Lissoclinum patella, but are now known to be ribosomally expressed by the blue-green algae Prochloron that live in symbiosis with L. patella. In a further step to unravel the metabolic significance of the patellamide complexes, the question as to whether these are also formed inside Prochloron cells is addressed. In this study, a biocompatible patellamide-fluorescent dye conjugate has been introduced into living Prochloron cells and, by means of flow cytometry and confocal microscopy, it is shown that CuIIions are coordinated to patellamides in vivo.
Universität Heidelberg, Anorganisch-Chemisches Institut and Interdisciplinary Center for Scientific Computing (IWR), Im Neuenheimer Feld 270, 69120, Heidelberg, Germany.
Peter
http://orcid.org/0000-0001-7796-3532
P
Comba
Universität Heidelberg, Anorganisch-Chemisches Institut and Interdisciplinary Center for Scientific Computing (IWR), Im Neuenheimer Feld 270, 69120, Heidelberg, Germany.
Annika
A
Eisenschmidt
School of Chemistry and Molecular Bioscience, University of Queensland, Brisbane, Queensland, 4072, Australia.
Lawrence R
LR
Gahan
CellNetworks Cluster und Physikalisch-Chemisches Institut, Universität Heidelberg, 69120, Heidelberg, Germany.
Dirk-Peter
DP
Herten
Independent Marine Biochemistry Research (IMBCR Pty. Ltd.), Point Lookout, Queensland, 4183, Australia.
Geoffrey
G
Nette
School of Chemistry and Molecular Bioscience, University of Queensland, Brisbane, Queensland, 4072, Australia.
Gerhard
G
Schenk
CellNetworks Cluster und Physikalisch-Chemisches Institut, Universität Heidelberg, 69120, Heidelberg, Germany.|Biochemistry Center (BZH), Universität Heidelberg, Im Neuenheimer Feld 345, 69120, Heidelberg, Germany.
Martin
M
Seefeld
copper; cyanobacteria; fluorescence; marine organisms; patellamides