Characterization ofSalmonellaGallinarum from an outbreak in Raigarh, Chhattisgarh.
OBJECTIVE
The present investigation was conducted to isolate and characterizeSalmonellaGallinarum from an outbreak of fowl typhoid in layer birds.


METHODS
Clinically ill and dead layer birds from an outbreak were investigated. History, clinical signs, and postmortem lesions were suggestive of fowl typhoid. Postmortem samples including heart blood, intestinal contents, pieces of ovary, and liver were collected and processed immediately for bacterial culture, serotyping and antibiotic sensitivity tests. Isolates were further screened for the presence of extended spectrum beta lactamase (ESBL) (blaTEM) gene by polymerase chain reaction.


RESULTS
On the basis of cultural, staining and biochemical characteristics; three bacterial isolates were confirmed as S. Gallinarum. On serotyping, somatic antigen O: 9 and 12 with nonflagellated antigen were detected in all three isolates. Isolates were intermediate sensitive to amoxycillin, amoxyclav, gentamicin and ciprofloxacin and resistant to most of the antibiotics including chloramphenicol, ampicillin, ceftazidime, cefexime, cefepime, azithromycin, nalidixin, tetracycline, oxytetracycline, and streptomycin. Two isolates were found to harbor ESBL (blaTEM) gene.


CONCLUSIONS
Beta lactamase producer S. Gallinarum was confirmed as cause of increased mortality in layer birds during present investigation. Existence of multi drug resistantSalmonellaposes serious threat to poultry industry in Chhattisgarh.
Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
Chandrahas
C
Sannat
Department of Veterinary Public Health, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
Anil
A
Patyal
Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
Nidhi
N
Rawat
Department of Veterinary Pathology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
R C
RC
Ghosh
Department of Veterinary Pathology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
D K
DK
Jolhe
Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
R K
RK
Shende
Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
S D
SD
Hirpurkar
Department of Veterinary Public Health, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
Sanjay
S
Shakya
O: 9; 12; Salmonella Gallinarum; blaTEM; poultry