Production, purification and characterization of halophilic organic solvent tolerant protease from marine crustacean shell wastes and its efficacy on deproteinization.
The quantum of marine fish wastes produced by fish processing industries has necessitated to search new methods for its disposal. Hence, this study is focused on production and purification of halophilic organic solvent tolerant protease (HOSP) from marine Alcaligenes faecalis APCMST-MKW6 using marine shell wastes as substrate. The candidate bacterium was isolated from the marine sediment of Manakudi coast and identified as A. faecalis APCMST-MKW6. The purified protease showed 16.39-fold purity, 70.34 U/mg specific activity with 21.67 % yield. The molecular weight of the purified alkaline protease was 49 kDa. This purified protease registered maximum activity at pH 9 and it was stable between pH 8-9 after 1.30 h of incubation. The optimum temperature registered was 60 °C and it was stable between 50 and 60 °C even after 1.30 h of incubation. This enzyme also showed maximum activity at 20 % NaCl concentration. Further, manganese chloride, magnesium chloride, calcium chloride and barium chloride influenced this enzyme activity remarkably and it was also found to be enhanced by many of the tested surfactants and solvents. The candidate bacterium effectively deproteinized the shrimp shell waste compared to the other tested crustaceans shell wastes and also attained maximum antioxidant activity.
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, 629 502, Tamilnadu, India.
Thirumalai
T
Maruthiah
Department of Zoology, Rani Anna Government College for Women, Manonmaniam Sundaranar University, Tirunelveli, 627 012, Tamilnadu, India.
Beena
B
Somanath
Department of Zoology, Muslim Arts College, Thiruvithancode, Kanyakumari, 629 174, Tamilnadu, India.
Jebamonydhas Vijila
JV
Jasmin
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, 629 502, Tamilnadu, India.
Grasian
G
Immanuel
Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli, 627 012, Tamilnadu, India. plavesh06@gmail.com.
Arunachalam
A
Palavesam
Antioxidants; Deproteinization; Halophilic organic solvent tolerant protease; Marine crustacean shell wastes; Stain removal