The establishment of appropriate methods for egg-activation by human PLCZ1 RNA injection into human oocyte.
Phospholipase C-zeta (PLCZ1), a strong candidate of egg-activating sperm factor, can induce Ca2+oscillations and cause egg activation. For the application of PLCZ1 to clinical use, we examined the pattern of Ca2+responses and developmental rate by comparing PLCZ1 RNA injection methods with the other current methods, such as cytosolic aspiration, electrical stimulation and ionomycin treatment in human oocytes. We found that the pattern of Ca2+oscillations after PLCZ1 RNA injection exhibited similar characteristics to that after ICSI treatment. We also determined the optimal concentration of human PLCZ1 RNA to activate the human oocytes. Our findings suggest that human PLCZ1 RNA is a better therapeutic agent to rescue human oocytes from failed activation, leading to normal and efficient development.
Saint Mother Obstetrics and Gynecology Clinic, Institute for ART, Fukuoka 807-0825, Japan; Department of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo 113-0033, Japan.
Takashi
T
Yamaguchi
Department of Virology and Parasitology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan. Electronic address: mito557@hama-med.ac.jp.
Masahiko
M
Ito
Department of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo 113-0033, Japan.
Keiji
K
Kuroda
Department of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo 113-0033, Japan.
Satoru
S
Takeda
Saint Mother Obstetrics and Gynecology Clinic, Institute for ART, Fukuoka 807-0825, Japan.
Atsushi
A
Tanaka
D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D006801:Humans; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C; D012313:RNA / Q000494:pharmacology*
D012313:RNA; C499235:PLCZ1 protein, human; D054801:Phosphoinositide Phospholipase C; D002118:Calcium
Ca(2+) oscillations; Egg activation; Human oocyte; PLCZ1 RNA; Preimplantation development.