IL-6, TNF-α, IL-10, and nutritional status in pediatric patients with biliary atresia.
OBJECTIVE
The objective of the present study is to evaluate whether IL-6, TNF-α, IL-10 are associated with nutritional status in patients with cirrhosis secondary to biliary atresia and compare to healthy controls.


METHODS
The parameters used for nutritional assessment were the standard deviation scores of height-for-age and of triceps skinfold thickness-for-age. The severity of cirrhosis was evaluated using the Child-Pugh score and PELD/MELD. Serum cytokines were measured using Cytometric Bead Array flow cytometry.


RESULTS
IL-6, TNF-α, and IL-10 were significantly higher in the cirrhosis group when compared with the control group (2.4 vs. 0.24 (p<0.001), 0.21 vs. 0.14 (p=0.007), and 0.65 vs. 0.36 (p=0.004), respectively. IL-6 and IL-10 were positively correlated with disease severity (0.450 [p=0.001] and 0.410; [p=0.002], respectively). TNF-α did not show a significant correlation with disease severity (0.100; p=0.478). Regarding nutritional evaluation, IL-6 was negatively correlated with the standard deviation score of height-for-age (-0.493; p<0.001) and of triceps skinfold thickness-for-age (-0.503; p<0.001), respectively. IL-10 exhibited a negative correlation with the standard deviation score of height-for-age (-0.476; p<0.001) and the standard deviation score of triceps skinfold thickness-for-age (-0.388; p=0.004). TNF-α did not show any significance in both anthropometric parameters (-0.083 (p=0.555) and -0.161 (p=0.253).


CONCLUSIONS
The authors suggest that, in patients with cirrhosis secondary to biliary atresia, IL-6 could be used as a possible supporting biomarker of deficient nutritional status and elevated IL-10 levels could be used as a possible early-stage supporting biomarker of deteriorating nutritional status.
Hospital de Clínicas de Porto Alegre, Centro de Pesquisa, Laboratório Experimental de Hepatologia e Gastroenterologia, Porto Alegre, RS, Brazil; Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Programa de Pós-graduação em Saúde da Criança e do Adolescente, Porto Alegre, RS, Brazil. Electronic address: ineswilasco@gmail.com.
Maria Ines de Albuquerque
MIA
Wilasco
Hospital de Clínicas de Porto Alegre, Centro de Pesquisa, Laboratório Experimental de Hepatologia e Gastroenterologia, Porto Alegre, RS, Brazil; Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Programa de Pós-graduação em Gastroenterologia, Porto Alegre, RS, Brazil.
Carolina
C
Uribe-Cruz
Universidade do Vale do Rio dos Sinos (Unisinos), Faculdade de Nutrição, São Leopoldo, RS, Brazil.
Daniele
D
Santetti
Hospital de Clínicas de Porto Alegre, Centro de Pesquisa, Laboratório de Psiquiatria Molecular, Porto Alegre, RS, Brazil.
Gabriel Rodrigo
GR
Fries
Hospital de Clínicas de Porto Alegre, Departamento de Pediatria, Porto Alegre, RS, Brazil.
Cristina Toscani Leal
CTL
Dornelles
Hospital de Clínicas de Porto Alegre, Centro de Pesquisa, Laboratório Experimental de Hepatologia e Gastroenterologia, Porto Alegre, RS, Brazil; Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Programa de Pós-graduação em Saúde da Criança e do Adolescente, Porto Alegre, RS, Brazil; Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Programa de Pós-graduação em Gastroenterologia, Porto Alegre, RS, Brazil.
Themis Reverbel da
TRD
Silveira
D001656:Biliary Atresia / Q000097:blood* / Q000150:complications / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000097:blood* / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood* / Q000276:immunology; D008103:Liver Cirrhosis / Q000097:blood* / Q000209:etiology / Q000276:immunology; D008297:Male; D015596:Nutrition Assessment; D009752:Nutritional Status; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000097:blood* / Q000276:immunology
D015415:Biomarkers; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
Atresia Biliar; Biliary atresia; Cirrhosis; Cirrose; Estado nutricional; IL-10; IL-6; Nutritional status; TNF-α