Use of supercharged cover screw as static magnetic field generator for bone healing, 1st part: in vitro enhancement of osteoblast-like cell differentiation.
Since 1979, Pulsed electromagnetic fields (PEMFs) have been approved by the Food and Drug Administration as an effective method in the treatment of non-unions. As well as PEMFs, also static magnetic fields (SMFs) have been widely investigated in orthopaedic studies. Even if the exact mechanism of action is not well understood, a large number of studies showed specific effects both at cellular and tissue levels. As bone fracture healing and osseointegration share the same biological events, the application of magnetic field stimulation in order to facilitate the osseointegration process has been suggested. In this study we investigated the proliferation rate and gene expression profile of MG63 osteoblastic-like cells after a 24, 48 and 72-hour SMF stimulation, generated by a small, customized cover screw-shaped neodymium-iron-bore magnet placed in the inner cavity of a dental implant. As a result, we found that the application of a SMF to osteoblastic-like cells does slightly decrease cell proliferation rate while enhancing the expression of those genes correlated to differentiation and mineralization. Our findings represent, to our knowledge, the first clinical ready technique for dental implants showing the ability of SMF to promote the osteogenesis process in vitro.
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Bambini
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Santarelli
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Putignano
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Procaccini
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Orsini
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Memè
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Sartini
Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
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Emanuelli
Department of Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
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Lo Muzio
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D001861:Bone Regeneration / Q000235:genetics*; D001863:Bone Screws; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D024121:Collagen Type X / Q000235:genetics / Q000378:metabolism; D015921:Dental Implants; D015870:Gene Expression; D006801:Humans; D060526:Magnetic Fields; D059346:Magnets; D016348:Osseointegration / Q000235:genetics*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D053402:PHEX Phosphate Regulating Neutral Endopeptidase / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
C525718:BMP2 protein, human; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; D024121:Collagen Type X; D015921:Dental Implants; D053773:Transforming Growth Factor beta1; D019010:Vascular Cell Adhesion Molecule-1; C548267:ALPL protein, human; D000469:Alkaline Phosphatase; D053402:PHEX Phosphate Regulating Neutral Endopeptidase; C506231:PHEX protein, human