[Influences of vibration on rapid osteogenic response of osteoblasts].
OBJECTIVE
This study investigated the rapid response of osteoblasts, which were derived from low-magnitude high-frequency vibration (LMHFV). Refractory period-derived memory response was also observed.


METHODS
MC3T3-E1 cells were incubated and received LMHFV stimulation (0.49 g, 40 Hz) for 30 min. After application of LMHFV, mRNA levels of earlier osteogenic differentiation markers Runt-related transcription factor 2 (Runx2), collagen typeⅠ(Col-Ⅰ), and alkaline phosphatase (ALP) were immediately detected by real-time fluorescence quantitative polymerase chain reaction in the absence or presence of antioxidant. Simultaneously, concentrations of mitochondrial reactive oxygen species (ROS) and average mitochondrial length were also measured.


RESULTS
Osteoblasts in the vibration group showed decreased gene expressions of Runx2, Col-Ⅰ, and ALP (P<0.01) and increased levels of mitochondrial ROS (P<0.01) and shortened mitochondria (P<0.01), whereas antioxidant treatment resulted in recovery from changes in the above indicators (P<0.01).


CONCLUSIONS
LMHFV can downregulate mRNA levels of early osteogenic differentiation markers, promote ROS generation, and mitochondrial fission.  .
State Key Laboratory of Oral Diseases, Dept. of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Zhu
Z
Zhuoli
State Key Laboratory of Oral Diseases, Dept. of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Zhang
Z
Ling
State Key Laboratory of Oral Diseases, Dept. of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Ma
M
Ruiyang
State Key Laboratory of Oral Diseases, Dept. of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Gan
G
Xueqi
D000469:Alkaline Phosphatase; D000818:Animals; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit; D015536:Down-Regulation; D051379:Mice; D010006:Osteoblasts; D010012:Osteogenesis; D014732:Vibration
D050659:Core Binding Factor Alpha 1 Subunit; D000469:Alkaline Phosphatase
mitochondrial morphology; osteogenic differentiation; reactive oxygen species; vibration