Interferon gamma and interleukin 10 polymorphisms in Chinese children with hemophagocytic lymphohistiocytosis.
OBJECTIVE
The aim of the study is to investigate the association of interferon gamma (IFN-γ) and interleukin-10 (IL-10) gene single nucleotide polymorphisms with the susceptibility of hemophagocytic lymphohistiocytosis (HLH) in Chinese children without known family history of HLH.


METHODS
Forty children with HLH and 160 age- and gender-matched healthy controls from Xuzhou Children's Hospital were enrolled in the study. Serum IFN-γ and IL-10 levels were measured by enzyme linked-immunosorbent assay. Polymorphisms of the IFN-γ gene at position +874 and +2109, and IL-10 at position -1082 were analyzed by allele-specific PCR.


RESULTS
Median serum concentrations of IFN -γ and IL-10 were significantly higher in children with HLH compared to healthy controls. The frequencies of IFN-γ +874 T/A and T/T genotypes, as well as T allele, were significantly higher in the HLH group compared with those in the control group. The frequencies of IL-10 -1082 G/A genotype and G allele were significantly increased in HLH patients compared with healthy controls. No significant difference was found in the distribution of IFN-γ +2109G/A genotypes between children with HLH and controls.


CONCLUSIONS
This study presents preliminary evidence for the association between IFN +874 T/A, T/T, IL-10 -1082 A/G genotypes, and HLH susceptibility in Chinese children with HLH.
Division of Hematology, Xuzhou Children's Hospital, Xuzhou, People's Republic of China.|Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, People's Republic of China.
Qi
Q
An
Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, People's Republic of China.
Shao-Yan
SY
Hu
Division of Hematology, Xuzhou Children's Hospital, Xuzhou, People's Republic of China.
Cheng-Min
CM
Xuan
Division of Hematology, Xuzhou Children's Hospital, Xuzhou, People's Republic of China.
Ming-Wei
MW
Jin
Division of Hematology, Xuzhou Children's Hospital, Xuzhou, People's Republic of China.
Qiang
Q
Ji
Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, People's Republic of China.
Yi
Y
Wang
D044466:Asian People / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics*; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D051359:Lymphohistiocytosis, Hemophagocytic / Q000097:blood / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
C508609:IL10 protein, human; D016753:Interleukin-10; D007371:Interferon-gamma
IFN-γ; IL-10; hemophagocytic lymphohistiocytosis (HLH); polymorphisms