Characterization of dormant and active human cancer cells by quantitative phase imaging.
The switch of tumor cells from a dormant, non-angiogenic phenotype to an active, angiogenic phenotype is a critical step in early cancer progression. To date, relatively little is known about the cellular behaviors of angiogenic and non-angiogenic tumor cell phenotypes. In this study, holographic imaging cytometry, a quantitative phase imaging (QPI) technique was used to continuously and non-invasively analyze, quantify, and compare a panel of fundamental cellular behaviors of angiogenic and non-angiogenic human osteosarcoma cells (KHOS) in a simple and economical way. Results revealed that angiogenic KHOS cells (KHOS-A) have significantly higher cell motility speeds than their non-angiogenic counterpart (KHOS-N) while no difference in their cell proliferation rates and cell cycle lengths were observed. KHOS-A cells were also found to have significantly smaller cell areas and greater cell optical thicknesses when compared with the non-angiogenic KHOS-N cells. No difference in average cell volumes was observed. These studies demonstrate that the morphology and behavior of angiogenic and non-angiogenic cells can be continuously, efficiently, and non-invasively monitored using a simple, quantitative, and economical system that does not require tedious and time-consuming assays to provide useful information about tumor dormancy. © 2017 International Society for Advancement of Cytometry.
Vascular Biology Program, Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.|Department of Surgery, Harvard Medical School and Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.
Peng
P
Guo
Vascular Biology Program, Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.|Department of Surgery, Harvard Medical School and Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.
Jing
J
Huang
Vascular Biology Program, Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.|Department of Surgery, Harvard Medical School and Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.
Marsha A
MA
Moses
D045744:Cell Line, Tumor; D019070:Cell Lineage / Q000235:genetics; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D006696:Holography / Q000379:methods*; D006801:Humans; D009389:Neovascularization, Pathologic / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology
cell cycle; cell migration; cell morphology; cell phenotype; holographic microscopy; osteosarcoma; quantitative phase imaging; tumor angiogenesis; tumor dormancy