A fluorescent probe based upon anthrancene-dopamine thioether for imaging Hg2+ions in living cells.
A novel anthrancene-dopamine thioether L compound was designed as fluorescent probe for detecting Hg2+in living cells sample. L exhibits a good sensitive and selective recognition towards Hg2+ions in the presence of other important relevant metal ions and amino acids in HEPES solution. The addition of Hg2+causes a marked enhancement in the fluorescence emission intensity with the detection limit as low as 1.1×10-6M, combining with obvious colormetric change from colorless to pale brown. Mechanistic studies show that catechol group and sulfur atom in L all participate in the coordination with Hg2+, though catechol group contributes mainly to chelation-enhanced fluorescence enhancement and sulfur atom to selectivity. Furthermore, L demonstrates good cell permeability and compatibility for sensitive fluorescent detection of Hg2+in HepG2 cells. This present probe will have broad applications in biological imaging.
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China.
Wenjie
W
Feng
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China.
Qing
Q
Xia
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China.
Haiyan
H
Zhou
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, PR China.
Yun
Y
Ni
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, PR China.
Liulin
L
Wang
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China.
Su
S
Jing
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, PR China.
Lin
L
Li
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China.
Wei
W
Ji
D004298:Dopamine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D061848:Optical Imaging / Q000379:methods*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000737:chemistry*
D005456:Fluorescent Dyes; D011084:Polycyclic Aromatic Hydrocarbons; D013440:Sulfides; D008628:Mercury; D004298:Dopamine
Anthrancene; Cellular imaging; Dopamine; Fluorescent probe; Hg(2+); Thioether