Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in recruitment of host fibronectin and plasminogen.
Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. Previous studies suggested glyceraldehyde 3-phosphate dehydrogenase (GAPDH) plays a role in the pathogenesis of E. rhusiopathiae infection. We studied E. rhusiopathiae GAPDH interactions with pig vascular endothelial cells, fibronectin, and plasminogen. Recombinant GAPDH (rGAPDH) was successfully obtained, and it was shown that it plays a role in E. rhusiopathiae adhesion to pig vascular endothelial cells. Moreover, rGAPDH could bind fibronectin and plasminogen in a dose-dependent manner. To our knowledge, this is the first study demonstrating that a moonlighting protein plays a role in pathogenesis of E. rhusiopathiae infections.
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Weifeng
W
Zhu
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|College of Life Sciences & Technology, Huazhong Agricultural University, Wuhan, China.
Qiang
Q
Zhang
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Jingtao
J
Li
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Yanmin
Y
Wei
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Chengzhi
C
Cai
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Liang
L
Liu
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Zhongmin
Z
Xu
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. jml8328@126.com.|College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. jml8328@126.com.|Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China. jml8328@126.com.|Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China. jml8328@126.com.
Meilin
M
Jin
D000818:Animals; D001422:Bacterial Adhesion; D042783:Endothelial Cells / Q000382:microbiology*; D004888:Erysipelothrix; D004889:Erysipelothrix Infections / Q000382:microbiology*; D005353:Fibronectins / Q000378:metabolism*; D005434:Flow Cytometry / Q000662:veterinary; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D005353:Fibronectins; D010958:Plasminogen; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases