Effect of Cd2+and Cd2+/auxin mixtures on lipid monolayers - Model membrane studies on the role of auxins in phytoremediation of metal ions from contaminated environment.
In this work Langmuir monolayer experiments were performed to analyze the effect of Cd2+ions and their mixtures with synthetic auxin (1-naphthaleneacetic acid - NAA) on lipid films. These investigations were motivated by the fact that auxins act effectively as the agents improving the removal of metal ions from contaminated water and soil by plants (phytoextraction), and although their mechanism of action in this area is still unclear, it was suggested that it can be membrane-related. The experiments were done for one component (1,2-dipalmitoyl-sn-glycero-3-phosphocholine - DPPC; 1,2-dioleoyl-sn-glycero-3-phosphocholine - DOPC; 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) - DPPG) monolayers and mixed (DPPG/DOPC and DPPG/DPPC) films treated as model of plant leaves membranes. The monolayer properties were analyzed based on the surface pressure-area isotherms obtained during film compression, stability measurements and Brewster angle microcopy studies. The collected results together with the data presented in literature evidenced that both metal ions and auxins modify lipid system properties and by using them in a combination it is possible to weaken the influence of sole metal ions on membrane organization. This seems to be in agreement with the hypothesis that the role of plant growth regulators in increasing phytoextraction effectiveness may be membrane-related. However, further experiments are required to find possible correlations between the type and concentration of metal ion, composition of membrane or structural elements in auxin molecule and observed alterations in membrane properties.
Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland. Electronic address: hac@chemia.uj.edu.pl.
Katarzyna
K
Hąc-Wydro
Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Marzena
M
Mach
Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Karolina
K
Węder
Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland.
Katarzyna
K
Pająk
Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Paweł
P
Wydro
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000633:toxicity*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008954:Models, Biological; D009280:Naphthaleneacetic Acids / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010944:Plants / Q000737:chemistry; D013500:Surface Tension; D013816:Thermodynamics; D053835:Unilamellar Liposomes / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D004785:Environmental Pollutants; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D053835:Unilamellar Liposomes; D002104:Cadmium; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; C034182:1-naphthaleneacetic acid; C017251:1,2-oleoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol
Auxins; Brewster angle microscopy; Cadmium ions; Model membrane; Phytoremediation