Designing Smart Materials with Recombinant Proteins.
Recombinant protein design allows modular protein domains with different functionalities and responsive behaviors to be easily combined. Inclusion of these protein domains can enable recombinant proteins to have complex responses to their environment (e.g., temperature-triggered aggregation followed by enzyme-mediated cleavage for drug delivery or pH-triggered conformational change and self-assembly leading to structural stabilization by adjacent complementary residues). These "smart" behaviors can be tuned by amino acid identity and sequence, chemical modifications, and addition of other components. A wide variety of domains and peptides have smart behavior. This review focuses on protein designs for self-assembly or conformational changes due to stimuli such as shifts in temperature or pH.
Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907-2100, USA.
Sydney
S
Hollingshead
Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907-2100, USA.
Charng-Yu
CY
Lin
Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907-2100, USA.|Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907-2032, USA.
Julie C
JC
Liu
D000818:Animals; D006801:Humans; D066329:Protein Aggregates; D000072417:Protein Domains; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics*
D066329:Protein Aggregates; D011994:Recombinant Proteins
biomaterials; biopolymers; hydrogels; self-assembly; stimuli-sensitive polymers