Exogenous H2S prevents high glucose-induced damage to osteoblasts through regulation of KATP channels.
Association between diabetes and osteoporosis has been extensively investigated. However, whether the effects of H2S on osteoblast function under high glucose (HG) condition relate to ATP-sensitive potassium (KATP) channels is unclear. This study aimed to investigate the effects of H2S on the proliferation, apoptosis, and mineralization of osteoblasts under HG condition, and observe whether H2S exert this effect through the KATP channels. Primary osteoblasts (POBs) were obtained from the calvaria of Sprague-Dawley rats. Cells were pre-treated with 400 μmol/L NaHS (a H2S donor), and/or 0.1 mmol/L non-selective KATP channel opener pinacidil (Pia), and/or KATP channel inhibitor glibenclamide (Gli). They then were cultured in DMEM with 26.5 mmol/L (HG) or 5.5 mmol/L (control) glucose. Cell proliferation, apoptosis, and mineralization were determined. In addition, the expression of KATP SUR1 was also determined. NaHS pretreatment significantly inhibited the down-regulation of KATP SUR1 expression in POBs. NaHS also significantly prevented osteoblast injury induced by HG, through decreasing the rate of cell proliferation and increasing the number of apoptotic cells, which was similar with the effects of Pia. In addition, NaHS significantly deterred the HG-induced POB mineralization inhibition. H2S prevents the HG-induced osteoblast damage through regulating KATP channels.
Department of Orthodontics, School of Stomatology, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, China; Department of Orthodontics, School of Stomatology, Weifang Medical University, Weifang, Shandong, China.
Yuanyuan
Y
Liu
Department of Orthopedics, Weifang People's Hospital, Weifang, Shandong, China.
Jianyong
J
Liu
Functional Laboratory of Weifang Medical University, Weifang, Shandong, China.
Xin
X
Li
Department of Orthodontics, School of Stomatology, Weifang Medical University, Weifang, Shandong, China.
Fantao
F
Wang
Department of Orthodontics, School of Stomatology, Weifang Medical University, Weifang, Shandong, China.
Xin
X
Xu
Department of Orthodontics, School of Stomatology, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, China. Electronic address: wangchl@sdu.edu.cn.
Chunling
C
Wang
D000393:Air Pollutants / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000633:toxicity*; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D054086:KATP Channels / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010006:Osteoblasts / Q000187:drug effects* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013549:Sweetening Agents / Q000633:toxicity
D000393:Air Pollutants; D054086:KATP Channels; D012333:RNA, Messenger; D013549:Sweetening Agents; D005947:Glucose; D006862:Hydrogen Sulfide
Diabetes; Hydrogen sulfide; KATP channels; Osteoblasts; Osteoporosis