A Portable Immunosensor with Differential Pressure Gauges Readout for Alpha Fetoprotein Detection.
A portable, affordable and simple detector is requested in a "Point-of-Care-Testing" (POCT) system. In this study, we exploited the potentialities of Differential Pressure Gauge (DPG) to the orientation of POCT technology. Alpha fetoprotein (AFP) was chosen as a model analyte that could specifically recognized by its antigen, and a tiny outfits equipped with a DPG was employed as the signal readout. Pt/SiO2nanospheres were synthesized and modified with the detection antibody. In the presence of target, a sandwich of immunocomplex specifically formed and the Pt/SiO2had been modified on the capture antibody. Which then can be dissolved to release plenty of Pt and the suspensions were transferred into a closed vial filled with appropriated amount of hydrogen peroxide. Subsequently, hydrogen peroxide was decomposed to produce oxygen, resulting in the enhancement of pressure in the closed vial and which can be detected by DPG easily. Under the optimized conditions, the read out signal from DPG had a direct relationship with AFP concentrations in the range of 10~200 ng/mL, and the detection limit was as low as 3.4 ng/mL. The proposed portable sensor had been successfully applied to detect AFP in serum samples with satisfactory results. This strategy holds a great promising in biological analysis as its convenient operations, reliable results and flexible apparatus.
College of Environmental Science and Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.|College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.
Qingping
Q
Wang
College of Environmental Science and Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.|College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.
Rongjie
R
Li
Department of Thoracis Surgery, Cancer Hospital &Institute, CAMS &PUMC, Beijing, 100021, China.
Kang
K
Shao
MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
Yue
Y
Lin
MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
Weiqiang
W
Yang
MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
Longhua
L
Guo
MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
Bin
B
Qiu
MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
Zhenyu
Z
Lin
MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
Guonan
G
Chen
D055910:Antibodies, Immobilized; D055664:Electrochemical Techniques; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D054118:Nanospheres / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D019095:Point-of-Care Systems; D012822:Silicon Dioxide / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D055910:Antibodies, Immobilized; D000509:alpha-Fetoproteins; D010984:Platinum; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide