Treatment effects and mechanisms of Yujin Powder on rat model of large intestine dampness-heat syndrome.
BACKGROUND
Yujin Powder (YJP), an old prescription, is one of the most classical prescription for treating the large intestine dampness-heat syndrome (LIDHS). However, its potential modern pharmacological mechanisms remain unclear.


OBJECTIVE
The present study was designed to explore the essence of LIDHS and treatment mechanisms of the YJP on the LIDHS.


METHODS
The rat model of LIDHS was established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment (HTHE), drinking and intraperitoneal injection of Escherichia coli., which imitated the inducing conditions of LIDHS. Then the clinical symptoms and signs, blood routine, blood biochemistry, whole blood viscosity (WBV), serum inflammatory cytokines levels and the histopathological changes of main organs were detected and observed, respectively.


RESULTS
The results showed that the clinical symptoms and signs of the model rats were consistent with the diagnostic criteria of LIDHS, moreover, there were obvious systemic inflammatory response and extensive congestion. And after treatment with YJP in different dosages, the clinical symptoms and signs of the rats with LIDHS were improved; the indexes of blood routine and blood biochemistry and inflammatory cytokines levels tended to be normal; the WBV decreased and histopathological changes of major organs were alleviated or returned to normal. There was an obvious dose-effect relationship, and the high dose of YJP (HD-YJP) had the best treatment effects.


CONCLUSIONS
These results suggested that in LIDHS, diarrhea was the major clinical manifestation; the large intestine was the main lesion area; mucosa injury, inflammation and congestion of the large intestine with systemic inflammatory response and congestion were the most typical pathological characteristics. Meanwhile, YJP exhibited the comprehensive effects of anti-diarrhea, anti-inflammation, lowering blood lipid, relieving blood stasis, repairing intestinal mucosa and regulation and protection of multiple organs on LIDHS. These findings provided not only important information for understanding the essence of LIDHS but also the theoretical basis for developing new-drugs for treating dampness-heat type of diarrheal diseases.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: kololo-2008@163.com.
Wanling
W
Yao
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: 1720601837@qq.com.
Chaoxue
C
Yang
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: 13659465092@163.com.
Yanqiao
Y
Wen
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: awang001@126.com.
Wangdong
W
Zhang
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: 770455708@qq.com.
Xiaosong
X
Zhang
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: mq652@qq.com.
Qi
Q
Ma
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: jipengjipeng528@163.com.
Peng
P
Ji
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: huayongli2004@163.com.
Yongli
Y
Hua
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: weiym@gsau.edu.cn.
Yanming
Y
Wei
D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects; D016207:Cytokines / Q000097:blood; D003967:Diarrhea / Q000188:drug therapy / Q000209:etiology; D059305:Diet, High-Fat; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D004927:Escherichia coli Infections / Q000150:complications / Q000382:microbiology; D005260:Female; D006358:Hot Temperature; D006813:Humidity; D007410:Intestinal Diseases / Q000188:drug therapy* / Q000382:microbiology; D007420:Intestine, Large / Q000382:microbiology / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose; D013577:Syndrome
D016207:Cytokines; D004365:Drugs, Chinese Herbal; C000621895:yujin powder; D013395:Sucrose
Diarrhea; Diarrheal diseases; Large intestine dampness-heat syndrome (LIDHS); Treatment mechanism; Yujin Powder (YJP)