Isolation and characterization of microsatellite loci forSmilax sieboldii(Smilacaceae).
OBJECTIVE
Polymorphic microsatellite markers were developed forSmilax sieboldii(Smilacaceae), a member of theS. hispidagroup with a biogeographic disjunction between eastern Asia and North America, to study the phylogeography and incipient speciation of this species and its close relatives.


RESULTS
Transcriptome sequencing produced 47,628 unigenes. Seventeen loci were developed from 122 randomly selected primer pairs. Polymorphism and genetic variation were evaluated for 68 accessions representing five populations ofS. sieboldii. The number of alleles per locus ranged from four to 18; the expected heterozygosity ranged from 0.59 to 0.92. Twelve loci were successfully amplified in five related species:S. scobinicaulis,S. californica,S. hispida,S. moranensis, andS. jalapensis.


CONCLUSIONS
These novel expressed sequence tag-derived microsatellite markers will facilitate further population genetic research ofS. sieboldiiand its close allies of theS. hispidagroup.
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of China.
Yalu
Y
Ru
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
Ruijing
R
Cheng
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
Jing
J
Shang
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of China.
Yunpeng
Y
Zhao
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of China.
Pan
P
Li
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of China.
Chengxin
C
Fu
Smilacaceae; Smilax sieboldii; eastern Asian and North American disjunction; microsatellite primers; transcriptome sequencing