DNA barcoding and species delimitation of Chaitophorinae (Hemiptera, Aphididae).
Chaitophorinae aphids are widespread across Eurasia and North America, and include some important agricultural and horticultural pests. So, accurate rapid species identification is very important. Here, we used three mitochondrial genes and one endosymbiont gene to calculate and analyze the genetic distances within different datasets. For species delimitation, two distance-based methods were employed, threshold with NJ (neighbor-joining) and ABGD (Automatic Barcode Gap Discovery), and two tree-based approaches, GMYC (General Mixed Yule Coalescent) and PTP (Poisson Tree Process). The genetic interspecific divergence was clearly larger than the intraspecific divergence for four molecular markers. COI and COII genes were found to be more suitable for Chaitophorinae DNA barcoding. For species delimitation, at least one distance-based method combined with one tree-based method would be preferable. Based on the data forChaitophorus salinigerandLaingia psammae, DNA barcoding may also reveal geographical variation.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichen West Road, Chaoyang District, Beijing 100101, P.R. China; College of Life Science, University of Chinese Academy of Sciences, Shijingshan District, Beijing 100049, P.R. China.
Xi-Chao
XC
Zhu
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichen West Road, Chaoyang District, Beijing 100101, P.R. China.
Jing
J
Chen
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichen West Road, Chaoyang District, Beijing 100101, P.R. China.
Rui
R
Chen
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichen West Road, Chaoyang District, Beijing 100101, P.R. China.
Li-Yun
LY
Jiang
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichen West Road, Chaoyang District, Beijing 100101, P.R. China.
Ge-Xia
GX
Qiao
Chaitophorinae; distance-based analysis; gnd; mitochondrial genes; tree-based analysis