Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience.
A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. ETOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.
Centre for Clinical Brain Sciences, Chancellors Building, 49 Little France Crescent, University of Edinburgh, Edinburgh EH16 4SB, UK; Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK. Electronic address: paul.desousa@ed.ac.uk.
Paul A
PA
De Sousa
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Rachel
R
Steeg
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Elisabeth
E
Wachter
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Kevin
K
Bruce
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Jason
J
King
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Marieke
M
Hoeve
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Shalinee
S
Khadun
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
George
G
McConnachie
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Julie
J
Holder
Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Augustenburger Platz, Berlin 13353, Germany.
Andreas
A
Kurtz
Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Augustenburger Platz, Berlin 13353, Germany.
Stefanie
S
Seltmann
Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Augustenburger Platz, Berlin 13353, Germany.
Johannes
J
Dewender
Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Augustenburger Platz, Berlin 13353, Germany.
Sascha
S
Reimann
UK Stem Cell Bank, Division of Advanced Therapies, National Institute for Biological Standards and Control, Medicines and Healthcare Products Regulatory Authority, Blanche Lane, South Mimms, Hertfordshire, ENG 3GQ, UK.
Glyn
G
Stacey
UK Stem Cell Bank, Division of Advanced Therapies, National Institute for Biological Standards and Control, Medicines and Healthcare Products Regulatory Authority, Blanche Lane, South Mimms, Hertfordshire, ENG 3GQ, UK.
Orla
O
O'Shea
UK Stem Cell Bank, Division of Advanced Therapies, National Institute for Biological Standards and Control, Medicines and Healthcare Products Regulatory Authority, Blanche Lane, South Mimms, Hertfordshire, ENG 3GQ, UK.
Charlotte
C
Chapman
UK Stem Cell Bank, Division of Advanced Therapies, National Institute for Biological Standards and Control, Medicines and Healthcare Products Regulatory Authority, Blanche Lane, South Mimms, Hertfordshire, ENG 3GQ, UK.
Lyn
L
Healy
Fraunhofer Institute for Biomedical Engineering (IBMT), Josef-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany; Molecular & Cellular Biotechnology/Nanotechnology, Saarland University, Campus, 66123 Saarbrücken, Germany.
Heiko
H
Zimmermann
European Collection of Authenticated Cell Cultures, Public Health England, Porton Down, Salisbury SP4 0JG, UK.
Bryan
B
Bolton
European Collection of Authenticated Cell Cultures, Public Health England, Porton Down, Salisbury SP4 0JG, UK.
Trisha
T
Rawat
European Collection of Authenticated Cell Cultures, Public Health England, Porton Down, Salisbury SP4 0JG, UK.
Isobel
I
Atkin
Barcelona Stem Cell Bank, Centre for Regenerative Medicine in Barcelona, C/Dr. Aiguader 88, 08003 Barcelona, Spain.
Anna
A
Veiga
Barcelona Stem Cell Bank, Centre for Regenerative Medicine in Barcelona, C/Dr. Aiguader 88, 08003 Barcelona, Spain.
Bernd
B
Kuebler
Andalusian Public Health Care System, Avda Conocimiento sn, 18100 Armilla, Granada, Spain.
Blanca Miranda
BM
Serano
Centre for Physiology and Pathophysiology, Institute for Neurophysiology, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
Tomo
T
Saric
Centre for Physiology and Pathophysiology, Institute for Neurophysiology, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
Jürgen
J
Hescheler
Institute of Reconstructive Neurobiology, LIFE & BRAIN Centre, University of Bonn, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany.
Oliver
O
Brüstle
Institute of Reconstructive Neurobiology, LIFE & BRAIN Centre, University of Bonn, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany.
Michael
M
Peitz
Institute of Reconstructive Neurobiology, LIFE & BRAIN Centre, University of Bonn, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany.
Cornelia
C
Thiele
Hubrecht Institute for developmental biology and stem cell research, Royal Netherlands Academy of Arts and Sciences (KNAW), Utrecht University, Department of Clinical Sciences of Companion Animals and UMC Utrecht, 3584CT Utrecht, The Netherlands.
Niels
N
Geijsen
Bioneer A/S, Kogle Alle 2, DK-2970 Hørsholm, Denmark.
Bjørn
B
Holst
Bioneer A/S, Kogle Alle 2, DK-2970 Hørsholm, Denmark.
Christian
C
Clausen
Institute for Genetic Medicine, University of Newcastle, Newcastle NE1 3BZ, United Kingdom.
Majlinda
M
Lako
Institute for Genetic Medicine, University of Newcastle, Newcastle NE1 3BZ, United Kingdom.
Lyle
L
Armstrong
AstraZeneca, R&D, Innovative Medicines, Discovery Sciences, Reagents and Assay Development, HC3006, Pepparedsleden 1, SE-431 83 Mölndal, Sweden.
Shailesh K
SK
Gupta
AstraZeneca, R&D, Innovative Medicines, Discovery Sciences, Reagents and Assay Development, HC3006, Pepparedsleden 1, SE-431 83 Mölndal, Sweden.
Alexander J
AJ
Kvist
AstraZeneca, R&D, Innovative Medicines, Discovery Sciences, Reagents and Assay Development, HC3006, Pepparedsleden 1, SE-431 83 Mölndal, Sweden.
Ryan
R
Hicks
AstraZeneca, R&D, Innovative Medicines, Discovery Sciences, Reagents and Assay Development, HC3006, Pepparedsleden 1, SE-431 83 Mölndal, Sweden.
Anna
A
Jonebring
AstraZeneca, R&D, Innovative Medicines, Discovery Sciences, Reagents and Assay Development, HC3006, Pepparedsleden 1, SE-431 83 Mölndal, Sweden.
Gabriella
G
Brolén
Janssen Research & Development (A Division of Janssen Pharmaceutica N.V), Neuroscience Therapeutic Area, Turnhoutseweg 30, 2340 Beerse, Belgium.
Andreas
A
Ebneth
Janssen Research & Development (A Division of Janssen Pharmaceutica N.V), Neuroscience Therapeutic Area, Turnhoutseweg 30, 2340 Beerse, Belgium.
Alfredo
A
Cabrera-Socorro
UCB Biopharma (since May 2014), Discovery Research, Chemin du Foriest, Braine l'Alleud B-1420, Belgium.
Patrik
P
Foerch
UCB Biopharma (since May 2014), Discovery Research, Chemin du Foriest, Braine l'Alleud B-1420, Belgium.
Martine
M
Geraerts
H. Lundbeck A/S, Ottiliavej 9, 2500 Valby, Denmark.
Tina C
TC
Stummann
University of Edinburgh School of Law, Old College, South Bridge, Edinburgh EH8 9YL, UK.
Shawn
S
Harmon
University of Edinburgh School of Law, Old College, South Bridge, Edinburgh EH8 9YL, UK.
Carol
C
George
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Ian
I
Streeter
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Laura
L
Clarke
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Helen
H
Parkinson
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Peter W
PW
Harrison
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Adam
A
Faulconbridge
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Luca
L
Cherubin
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Tony
T
Burdett
Inbiomed, P° Mikeletegi, 81, 20009 San Sebastián, Gipuzkoa, Spain.
Cesar
C
Trigueros
Cellular Generation and Phenotyping (CGaP) facility, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinston CB10 1SA, UK.
Minal J
MJ
Patel
Cellular Generation and Phenotyping (CGaP) facility, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinston CB10 1SA, UK.
Christa
C
Lucas
Douglas Connect, Technology Park Basel, Hochbergerstrasse 60C, 4057 Basel, Switzerland.
Barry
B
Hardy
Douglas Connect, Technology Park Basel, Hochbergerstrasse 60C, 4057 Basel, Switzerland.
Rok
R
Predan
Douglas Connect, Technology Park Basel, Hochbergerstrasse 60C, 4057 Basel, Switzerland.
Joh
J
Dokler
Douglas Connect, Technology Park Basel, Hochbergerstrasse 60C, 4057 Basel, Switzerland.
Maja
M
Brajnik
Fraunhofer IME ScreeningPort, Schnackenburgallee 114, D-22525 Hamburg, Germany.
Oliver
O
Keminer
Fraunhofer IME ScreeningPort, Schnackenburgallee 114, D-22525 Hamburg, Germany.
Ole
O
Pless
Fraunhofer IME ScreeningPort, Schnackenburgallee 114, D-22525 Hamburg, Germany.
Philip
P
Gribbon
Fraunhofer IME ScreeningPort, Schnackenburgallee 114, D-22525 Hamburg, Germany.
Carsten
C
Claussen
ARTTIC, 58A rue du Dessous des Berges, F-75013 Paris, France.
Annette
A
Ringwald
ARTTIC, 58A rue du Dessous des Berges, F-75013 Paris, France.
Beate
B
Kreisel
Roslin Cells Ltd(1), Head office, Nine Edinburgh Bioquarter, 9 Little France Rd, Edinburgh EH16 4UX, UK; EBiSC banking facility, Babraham Research Campus, B260 Meditrina, Cambridge CB22 3AT, UK.
Aidan
A
Courtney
Pfizer Ltd (Neusentis), The Portway Building, Granta Park, Great Abington, Cambridge, CB21 6GS, UK.
Timothy E
TE
Allsopp
D018070:Biological Specimen Banks; D002460:Cell Line; D015925:Cryopreservation; D005060:Europe; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*