Oleanane-type Saponins from Glochidion hirsutum and Their Cytotoxic Activities.
Five new oleanane-type saponins, hirsutosides A - E, were isolated from the leaves of Glochidion hirsutum (Roxb.) Voigt. Their structures were elucidated as 21β-benzoyloxy-3β,16β,23,28-tetrahydroxyolean-12-ene 3-O-β-d-glucopyranoside (1), 21β-benzoyloxy-3β,16β,23,28-tetrahydroxyolean-12-ene 3-O-β-d-glucopyranosyl-(1 → 3)-β-d-glucopyranoside (2), 21β-benzoyloxy-3β,16β,23,28-tetrahydroxyolean-12-ene 3-O-6-acetyl-[β-d-glucopyranosyl-(1 → 3)]-β-d-glucopyranoside (3), 21β-benzoyloxy-3β,16β,23,28-tetrahydroxyolean-12-ene 3-O-β-d-glucopyranosyl-(1 → 3)-〈-l-arabinopyranoside (4), and 21β-benzoyloxy-3β,16β,23-trihydroxyolean-12-ene-28-al 3-O-β-d-glucopyranosyl-(1 → 3)-α-l-arabinopyranoside (5). All isolated compounds were evaluated for cytotoxic activities on four human cancer cell lines, HepG-2, A-549, MCF-7, and SW-626 using the SRB assay. Compounds 1, 2, 4, and 5 showed significant cytotoxic activities against all human cancer cell lines with IC50values ranging from 3.4 to 10.2 μm. Compound 3 containing acetyl group at glc C(6″) exhibited weak cytotoxic activity with IC50values ranging from 47.0 to 54.4 μm.
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Nguyen Van
NV
Thang
Faculty of Basic Sciences, Hanoi University of Mining and Geology, Tuliem, Hanoi, Vietnam.
Vu Kim
VK
Thu
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Nguyen Xuan
NX
Nhiem
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Duong Thi
DT
Dung
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Tran Hong
TH
Quang
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Bui Huu
BH
Tai
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Hoang Le Tuan
HLT
Anh
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Pham Hai
PH
Yen
Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Nguyen Thi Thanh
NTT
Ngan
Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Nguyen Huy
NH
Hoang
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Phan Van
PV
Kiem
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D018515:Plant Leaves / Q000737:chemistry; D012503:Saponins / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; C413246:oleanane; D009828:Oleanolic Acid

Glochidion hirsutum
; Cytotoxic activities; Euphorbiaceae; Hirsutosides A - E; Oleanane-type saponins