A nano-delivery system for bioactive ingredients using supercritical carbon dioxide and its release behaviors.
For the purpose of ensuring the bioavailability of bioactive ingredients, a nano-delivery system with low toxicity was developed using supercritical carbon dioxide (SC-CO2). Compared to thin-film hydration (TFH), obtaining nano-scale liposomes is easier using SC-CO2. The characteristic of these liposomes was also demonstrated by the analysis of particle size and morphology. An in vitro release study showed that liposomes produced using SC-CO2were resistant to low pH in simulated gastric conditions. In a simulated intestinal environment, enteric solubility of these liposomes was enhanced, which are important properties for controlled releasing bioactive ingredient. Furthermore, SC-CO2-produced liposomes had a higher storage stability than those produced using TFH. Analysis of the organic solvent residue in the liposomes by gas chromatography-mass spectrometry (GC-MS) indicated that SC-CO2-produced liposomes had lower toxicity than those produced by TFH. A chemical free nano-delivery system using SC-CO2has been revealed for storage and controlled release of bioactive ingredients.
College of Food Science, South China Agricultural University, Guangzhou 510640, China. Electronic address: situwenbei@scau.edu.cn.
Wenbei
W
Situ
College of Food Science, South China Agricultural University, Guangzhou 510640, China. Electronic address: songxl2000@163.com.
Xianliang
X
Song
College of Food Science, South China Agricultural University, Guangzhou 510640, China. Electronic address: luosc@scau.edu.cn.
Shucan
S
Luo
College of Food Science, South China Agricultural University, Guangzhou 510640, China. Electronic address: estgf123@qq.com.
Yan
Y
Liang
D001682:Biological Availability; D002245:Carbon Dioxide / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D008081:Liposomes; D053758:Nanoparticles / Q000737:chemistry*
D004337:Drug Carriers; D008081:Liposomes; D002245:Carbon Dioxide
Bioactive ingredients; Cholesterol (PubChem CID: 5997); Controlled release; Liposome; Melatonin (PubChem CID: 896); Nano-delivery system; Phosphatidylcholine (PubChem CID: 45266626); Supercritical carbon dioxide technique; Thin-film hydration