Structure of the N-glycans from the chlorovirus NE-JV-1.
Results from recent studies are breaking the paradigm that all viruses depend on their host machinery to glycosylate their proteins. Chloroviruses encode several genes involved in glycan biosynthesis and some of their capsid proteins are decorated with N-linked oligosaccharides with unique features. Here we describe the elucidation of the N-glycan structure of an unusual chlorovirus, NE-JV-1, that belongs to the Pbi group. The host for NE-JV-1 is the zoochlorella Micractinium conductrix. Spectroscopic analyses established that this N-glycan consists of a core region that is conserved in all of the chloroviruses. The one difference is that the residue 3OMe-L-rhamnose is acetylated at the O-2 position in a non-stoichiometric fashion.
Department of Chemical Sciences, University of Napoli, 80126, Naples, Italy. immacolata.speciale@unina.it.
Immacolata
I
Speciale
Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska, Lincoln, NE, 68583-0900, USA.
Irina
I
Agarkova
Department of Biology, Nebraska Wesleyan University, Lincoln, NE, USA.
Garry A
GA
Duncan
Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska, Lincoln, NE, 68583-0900, USA.
James L
JL
Van Etten
Department of Agricultural Sciences, University of Napoli, 80055, Portici, Italy.
Cristina
C
De Castro
D036022:Capsid Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002708:Chlorella / Q000821:virology; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D009844:Oligosaccharides / Q000737:chemistry; D017862:Phycodnaviridae / Q000737:chemistry* / Q000648:ultrastructure; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D066244:Proton Magnetic Resonance Spectroscopy; D012210:Rhamnose / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification
D036022:Capsid Proteins; D006020:Glycopeptides; D009844:Oligosaccharides; D011134:Polysaccharides; D014764:Viral Proteins; D012210:Rhamnose
Chlorovirus; Micractinium conductrix; NMR analysis; Pbi virus; Virus NE-JV-1