Efficient Discovery of Quality Control Markers for Gastrodia elata Tuber by Fingerprint-Efficacy Relationship Modelling.
BACKGROUND
Gastrodia elata tuber (GET) has been widely used in China as a famous herbal medicine. However, the quality control markers (QCMs) for GET still need further investigation.


OBJECTIVE
To develop a rational strategy based on fingerprint-efficacy relationship modelling to discover the efficacy-related QCMs, using GET as a case study.


METHODS
The high-performance liquid chromatography (HPLC) fingerprints of 13 batches of GET extracts were established and 10 common peaks were structurally characterised by HPLC coupled with quadrupole time-of-flight mass spectrometry (QTOF-MS). The neuroprotective effects of GET samples were evaluated using the in vitro model of β-amyloid peptide (Aβ25-35)-induced PC12 cell death. The fingerprint-efficacy relationship of chemical fingerprints and neuroprotective effects was linked by orthogonal projection to latent structure-discriminant analysis (OPLS-DA) model.


RESULTS
The chemical combination of 5-hydroxymethyl-2-furaldehyde (5-HMF), parishin B (PB) and parishin C (PC) was discovered and confirmed as QCMs of GET by the OPLS-DA method. The selected QCMs allowed to evaluate the relative quality of GET samples.


CONCLUSIONS
This study has demonstrated that the rational strategy based on fingerprint-efficacy relationship modelling might be generally applicable for the quality control of herbal medicines. Copyright © 2017 John Wiley & Sons, Ltd.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Mingfang
http://orcid.org/0000-0002-7754-225X
M
Li
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Yuan
Y
Du
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Long
L
Wang
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Lilong
L
Jiang
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Xiaodong
X
Ma
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Ping
P
Zhou
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Ping
P
Li
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Huijun
H
Li
D016229:Amyloid beta-Peptides; D000818:Animals; D002460:Cell Line; D002681:China; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031668:Gastrodia / Q000737:chemistry*; D010446:Peptide Fragments; D035281:Plant Tubers / Q000737:chemistry*; D011786:Quality Control; D051381:Rats
D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35)
Gastrodia elata tuber; HPLC-QTOF-MS; fingerprint-efficacy relationship modelling; neuroprotectivity; quality control markers