Deep-brain imaging via epi-fluorescence Computational Cannula Microscopy.
Here we demonstrate widefield (field diameter = 200 μm) fluorescence microscopy and video imaging inside the rodent brain at a depth of 2 mm using a simple surgical glass needle (cannula) of diameter 0.22 mm as the primary optical element. The cannula guides excitation light into the brain and the fluorescence signal out of the brain. Concomitant image-processing algorithms are utilized to convert the spatially scrambled images into fluorescent images and video. The small size of the cannula enables minimally invasive imaging, while the long length (>2 mm) allow for deep-brain imaging with no additional complexity in the optical system. Since no scanning is involved, widefield fluorescence video at the native frame rate of the camera can be achieved.
Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT 84112, USA.
Ganghun
G
Kim
Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA.
Naveen
N
Nagarajan
Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA.
Elissa
E
Pastuzyn
Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA.
Kyle
K
Jenks
Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA.
Mario
M
Capecchi
Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA.
Jason
J
Shepherd
Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT 84112, USA.
Rajesh
R
Menon
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D000072601:Cannula; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D059906:Neuroimaging; D019047:Phantoms, Imaging; D051381:Rats