Monoclonal Antibodies Production Against a 40KDa Band of Hydatid Cyst Fluid.
OBJECTIVE
Hydatid cyst is the larval stage of the tapeworm Echinococcus granulosus. Hydatid cyst fluid, cyst membrane and Protoscolices, contain a complex mixture of antigens that can induce immune responses in the host. Anti-cancer properties of Protoscolices and hydatid cyst fluid has been shown. In order to identify antigens of hydatid cyst fluid that have anti-cancer effect, in this study production of monoclonal antibodies against one of the hydatid cyst fluid band (40KDa) has been investigated. There are many published patents about applications of monoclonal antibodies.


METHODS
In this experimental study, 40KDa band of hydatid cyst fluid that has cross reaction with sera of patients with breast cancer was used as antigen. A group of mice were immunized with this antigen, and then their spleen cells were extracted and fused with SP2 cells. Monoclonal antibodies production was checked in wells with signs of cell growth using ELISA and western blotting. The reaction of the produced monoclonal antibodies with breast cancer cells was tested using flow cytometry method. Finally, effect of the monoclonal antibodies on growth of breast cancer cells was investigated in vitro.


RESULTS
The results of this study showed that in the first plate antibody against 40KDa was detected in several wells. In the second plate monoclonal antibodies with high titer was detected in one well. The produced monoclonal antibodies reacted with the surface of breast cancer cells. However, they had no significant effect on growth of breast cancer cells in culture medium.


CONCLUSIONS
Monoclonal antibodies against hydatid cyst fluid 40KDa band were produced. These antibodies reacted with the surface of breast cancer cells but had no significant effect on growth of these cells.
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan. Iran.
Seyedeh Maryam
SM
Sharafi
Department of Immunology, Shahrekord University of Medical Sciences, Shahrekord. Iran.
Hedayat
H
Shirzad
Department of Genetics, Isfahan University of Medical Sciences, Isfahan. Iran.
Hossein
H
Khanahmad
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan. Iran.
Behrooz
B
Ataei
Cancer Prevention Research Centre, Isfahan University of Medical Sciences, Isfahan. Iran.
Hossein Yousofi
HY
Darani
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D000936:Antigen-Antibody Complex / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000737:chemistry / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004443:Echinococcosis / Q000276:immunology* / Q000378:metabolism; D048209:Echinococcus granulosus / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000737:chemistry / Q000276:immunology; D007114:Immunization; D007814:Larva / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010330:Patents as Topic; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth; D000951:Antigens, Neoplasm
Monoclonal antibodies; anti-tumor; consumption; hydatid cyst antigens; parasites; tapeworm