DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity.
We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.
Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N-5020, Bergen, Norway.
Håvard
H
Foyn
Department of Biochemistry and Molecular Pharmacology, UMASS Medical School, 365 Plantation Street, Worcester, MA, 01605, USA.
Paul R
PR
Thompson
Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N-5020, Bergen, Norway. Thomas.Arnesen@uib.no.|Department of Surgery, Haukeland University Hospital, N-5021, Bergen, Norway. Thomas.Arnesen@uib.no.
Thomas
T
Arnesen
D000105:Acetyl Coenzyme A / Q000737:chemistry / Q000378:metabolism; D000107:Acetylation; D004228:Dithionitrobenzoic Acid / Q000737:chemistry*; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D066298:In Vitro Techniques; D063206:N-Terminal Acetyltransferases / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
D000105:Acetyl Coenzyme A; D004228:Dithionitrobenzoic Acid; D063206:N-Terminal Acetyltransferases
5,5′-dithiobis-(2-nitrobenzoic acid); Ac-CoA; Acetyltransferase; Enzyme activity; Enzyme assay; N-terminal acetyltransferase (NAT); Thiol detection; Thiol quantification