Allium stipitatumExtract Exhibits In Vivo Antibacterial Activity against Methicillin-ResistantStaphylococcus aureusand Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model.
The in vivo antibacterial and burn wound healing potency of Persian shallot bulbs (Allium stipitatum) were explored in a mice burn model infected with methicillin-resistantStaphylococcus aureus(MRSA). Hexane (ASHE) and dichloromethane (ASDE) extracts were tested. Female BALB/c mice were inflicted with third-degree thermal injury followed by infection with MRSA. ASHE and ASDE formulated with simple ointment base (SOB) at concentrations of 1%, 2%, and 5% (w/w) were topically applied to burn wounds twice a day for 20 days. Silver sulfadiazine (1%) served as drug positive control. Microbiological analysis was carried out on 1, 2, 3, 4, and 5 days postwounding (dpw) and histopathological analysis at the end of the experiment (20 dpw). Both ointments demonstrated strong antibacterial activity with complete elimination of MRSA at 48-72 h after infection. The rate of wound contraction was higher (95-100%) in mice groups treated with ASHE and ASDE ointments after 15 dpw. Histological analysis revealed significant increase (p< 0.05) in epithelialization and collagenation in treated groups. The ASHE and ASDE were found to be relatively noncytotoxic and safe to Vero cell line (383.4μg mL-1; 390.6μg mL-1), suggesting the extracts as safe topical antibacterial as well as promising alternatives in managing thermal injuries.
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Darul Ehsan, Malaysia.
Arunkumar
A
Karunanidhi
Department of Microbiology & Immunology, Faculty of Medicine, Arak University of Medical Sciences, Basij Square, Arak 38481-7-6941, Iran.
Ehsanollah
0000-0002-8484-7166
E
Ghaznavi-Rad
School of Medicine, Taylor's University, Lakeside Campus, No. 1, Jalan Taylor's, Subang Jaya, 47500 Selangor, Darul Ehsan, Malaysia.
Jayakayatri
J
Jeevajothi Nathan
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Darul Ehsan, Malaysia.
Yusuf
0000-0001-7888-9004
Y
Abba
BioMérieux, Scientific Office, La Balme les Grottes, France.
Alex
A
van Belkum
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Darul Ehsan, Malaysia.
Vasanthakumari
0000-0002-4076-8335
V
Neela