Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophila.
In the present work, we investigated molecular mechanisms governing thermal resistance of a monoxenous trypanosomatid Crithidia luciliae thermophila, which we reclassified as a separate species C. thermophila. We analyzed morphology, growth kinetics, and transcriptomic profiles of flagellates cultivated at low (23°C) and elevated (34°C) temperature. When maintained at high temperature, they grew significantly faster, became shorter, with genes involved in sugar metabolism and mitochondrial stress protection significantly upregulated. Comparison with another thermoresistant monoxenous trypanosomatid, Leptomonas seymouri, revealed dramatic differences in transcription profiles of the two species with only few genes showing the same expression pattern. This disparity illustrates differences in the biology of these two parasites and distinct mechanisms of their thermotolerance, a prerequisite for living in warm-blooded vertebrates.
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Aygul
A
Ishemgulova
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Anzhelika
A
Butenko
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
Lucie
L
Kortišová
Coleção de Protozoários, Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Carolina
C
Boucinha
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
Anastasiia
A
Grybchuk-Ieremenko
Coleção de Protozoários, Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.|Instituto de Biologia Roberto Alcântara Gomes, Departamento de Ecologia, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil.
Karina A
KA
Morelli
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Martina
M
Tesařová
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
Natalya
N
Kraeva
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Danyil
D
Grybchuk
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
Tomáš
T
Pánek
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.|Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia.
Pavel
P
Flegontov
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.|Faculty of Sciences, University of South Bohemia, České Budějovice (Budweis), Czech Republic.|Canadian Institute for Advanced Research, Toronto, Ontario, Canada.
Julius
J
Lukeš
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.|Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
Jan
J
Votýpka
Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Márcio Galvão
MG
Pavan
de Duve Institute, Université Catholique de Louvain, Brussels, Belgium.
Fred R
FR
Opperdoes
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia.
Viktoria
V
Spodareva
Coleção de Protozoários, Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Claudia M
CM
d'Avila-Levy
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia.
Alexei Yu
AY
Kostygov
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.|Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, United States of America.|Institute of Environmental Technologies, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
Vyacheslav
http://orcid.org/0000-0003-4765-3263
V
Yurchenko
D000818:Animals; D001669:Biochemical Phenomena / Q000235:genetics; D003421:Crithidia / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D007313:Insecta / Q000235:genetics*; D013696:Temperature; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics